LOCUS       DASM01000001            5974 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_32862, whole genome shotgun sequence.
ACCESSION   DASM01000001 DASM01000000
VERSION     DASM01000001.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_32862"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1151 a         1952 c         1932 g          937 t
ORIGIN      
        1 agggcgtcgg ggacctcgac gatgtgcttt atgaggtggt cagcccgata gatctcgccg
       61 cactcccggc actcggtgag ggggtcggag aagccggata ggtgtcccga ggccttgaag
      121 atctcctcca ccccgatggt ggggcactcg atctcgcaga acccctcccg gatgacgaag
      181 aaccccctcc agaggtcctc gatccccctc ttgagcgggg ctcccagggg gccgtaatcg
      241 tagaagcccg cagctccgcc gtagagctcg aaggcgggcc agatgaagcc ccgcctcctg
      301 gcaagctcga tcaccttctc gtacgtgtcc ataagccaat cctcgcctca cctagaccgc
      361 aagagcccat ttaacctttg tcgtacggct cctggggtct caatccgaat aataggcggc
      421 agatcctcga gatcgccccg gcgccggggt gggcgggggc ggggcgccgg gggcctcccc
      481 tatccgacag agtttttaag taggtgagcg tatcagcggt cggtgagtga taactcatga
      541 aagagatcat aaagttaggg ttagtgatgg gcgttctgtt ctgcgccgct atcggcgccg
      601 ccagcgctca agaggagtac tctgaggact acaagaacgg attctacgac gccgccgtgg
      661 tcatcggcca ggccaccggg atgggcggga gcctggagca gctctacaac gctctgggcg
      721 gagaagccac cccggagacg gcagacatcg tcgagtacta caacaaccag accaccctct
      781 tcaaccagca ggtgatcccc tacgtgaacg gagtcatcga ccaggtcttc ggccccgatg
      841 acaaccggtc cgcggaccta tatctggtgg agcttcccct catatcctga ttttggagat
      901 gatggccggg gaggcggaat gcaccgcccc ggccacctcc aaccatattc catcctcggc
      961 taaattccgg actttagagc gagcgcccca tttttgttcc gccaccttcc ccggggagcc
     1021 ccctagcttc cgtcctcgat agatcccagg agaggctcag accccggtca ggaccgtctc
     1081 ttggagaaag atcccccgca gttccagctc ccggaggtac cgctcgtaga ccgccccccg
     1141 gataccgtcc tcggggggga cgatcccccg cccccggagt tcgccgcggc cgaggatcct
     1201 cgcggccacg gccgccgagg agcccgtcac cctcgccatc gccgatacgc cagccaccag
     1261 atcggggccg gcgaggagga ggtggtcgac cctggccgcc tcccccccct tcagcccctg
     1321 gacggagttt gccatgatgc agaggtcggt ctcccccggc agaggccgga gcctcggctc
     1381 caccaccgag atgaagagct cccggggcct cactgagcag ccggcgacct ccatcggctc
     1441 aagatccaac aggccgcaac gccggaggag gtcgatcccc tcccagtgcc ccggccaccg
     1501 gaccgtcttc tcggtgaact cggcgaggcc cggcctcgta tagagaaggc tcggcatccc
     1561 gggggtggcg gcggcctcga actcctcccc caggggctcg aagacgaacc tctcccggtc
     1621 cgtcaggggg gcgacctcga cgatcctccc gccccggagg accctcggcc ggaccatgta
     1681 ctcccggagg acgtgcccga aggaccaggt gacggtgtac cggagagggt gattggccgc
     1741 cgcctccttc gaggggacgc ctccgacccg ggcggtggcc ctctccgcag agtcgaggag
     1801 gtcgatcccc cggccgatgg aggcgttcga gagccccggg gcaaacccca tcccgtcgag
     1861 gagggtgacg ccgttctcct ccgccctccg gtgaagggat tcgccgtact cgtccggggt
     1921 catgccggcc gggacgggga gcccctccgt ctcgtagggg tcggggcgcc ggtggtactc
     1981 ctccaggacg tcgacggcgt cgagggaggc gtcgatggcc gcctccaggg cccggtagct
     2041 cgtcctccga tcggggaggg cgatgacccc gacgtcgtag ccctccatca gccgccagac
     2101 ctcccgggcg tcgcccccgc cgacgtcgca aggatggagg cggagcttgt cgcccccgat
     2161 ccaggcggcg gccctctcca gccgccccgc cctggggccg acgagcccca ccacctccac
     2221 ctcgccggag cccgccaggt cccgggccgc cgccgacccg atcctgccgg cccctccaat
     2281 gaccagcacc ctcatattcc agcacctccc caacccccgt cctctcttgg catatcatca
     2341 cctcttttga ggttctcgtc ccctatttta tatcaaggac ccgccgggtg gtgagaggcg
     2401 ggagacggga gatggggcca actatctcgg cctgctgggg gcccgggcgc cgccccaccc
     2461 ggcaacggct ttccggcggg gctttgcgcc cttcgcccct cgatcatgtc gaaacctatt
     2521 tagaccacct ccttgcttgt ggtggcgata ctatgatcgg cgtcttaggg ttggaagacg
     2581 gcaccagggt cgaaggggtg ggcttcggnn gagctggtct tcgtgacggt gatgtccggc
     2641 tacgaggagg cactcactga tccctcctac aagggacagc ttttgacgtt cacctacccc
     2701 ctcatgggta actacggggt gagcggcgac cactttcagt cagaccggat cggtgccgcc
     2761 gccatggtct gcaaggagat ctggaacagg cccctccacc accggtcgac gaggacgata
     2821 tcccagttcc tggaggagga gggggtcccc ggaatccacg gtctggacac gaggatgctc
     2881 accatcaaga ttcgaaatca gggggctatg agggctgccc tcgccgtcgg cgaggggtcg
     2941 gatgtgatcg acgaggacgt cgtcggcctc gcaaaagatc agccggacat atcgagcctc
     3001 gacctgataa gcgccgtctc ctgcaaaagt ccctaccgga tccccggaga cggcccgaag
     3061 gtggtgatga tggacctggg gatcaagagg aacatcctcg actccatggc gtgccagggc
     3121 tttgacatag tggtggtgcc ttcggactcg aggatatcgg atatcgagag gtacgatcca
     3181 gaagccttat tcatatccaa cggccccggc gaccccgaaa aggccgggcg ggcgatcgag
     3241 gcggtgaagc acttcgcggg ggagaagccg gtcttcggaa tatgtctggg gctccaggtg
     3301 atctgcctcg ccctgggggc ggagacctac aagctgaagt tcggccacca cggcggaaac
     3361 cagccggtga aggacctgga gagcgggatc gtctacataa catcccagaa ccacaacttc
     3421 gccttgcgcc cccagtcggc ggaggggacc ggcctcaaga tcacccaggt caacgccaac
     3481 gacggaaccg tggaggcggt caggagcagc tacctgggga tggaggcggt ccagtaccac
     3541 cctgaggcga ggcccggccc cctctgcacc cgggagaggt tcttctcctc cgtggcgaaa
     3601 gagattgaga agaggaggag gaggtgaagg agatgccaaa gaggccagag ataaagaagg
     3661 tcctcctgat aggatcgggg ccgatccaga tcggccaggc ggcggagttc gacttctccg
     3721 gatcgcaggc ctgcaagtcc ctccgggagg agggggtgga ggtggtcctc gtcaactcca
     3781 accccgccac catcatgacc gacccggaga tggcggacag gatctacatc gagcccctcg
     3841 tccccgaggt cgtcgccaag atcatcgagc gggagcggcc cgacgggatc atcgccggga
     3901 tcggggggca gaccggcctg aacgtcacca gcgagctcgc cgagggaggg gtcctggaga
     3961 ggttcggggt cgaggttctc gggacgtccg tctccgccat ccgggagacg gaggaccggg
     4021 acctcttcaa gagcgccatg atcaggatcg gcgagccggt ccccaggagc cgggccgtcg
     4081 actccccgga gggggcgaag gaggctctga aggaattggg cctccccctc atcatcaggc
     4141 ccgcctacac cctcggcggc tccggggggg gctcggcgga gacggaggag gattacttca
     4201 ggatcgtcga gatggggatg aagaggtccc ggatccatca ggtcctggtg gaggagagcc
     4261 tcgtcggctg gacggagctg gagtacgagg tgatgaggga cagaaacgac acctgcatca
     4321 ccatctgcaa catggagaat atggacccca tggggatcca caccggcgaa tcgatcgttg
     4381 taacccccat ccagaccctc accgacgagg aggttcagac cctccgcacc gccgccataa
     4441 acatcatccg cgccctcaag atcgaggggg ggtgcaacat ccagttcgcg gcgaaggacg
     4501 gcgagtaccg ggtgatcgag gtgaaccccc gggtctccag aagctccgcc ctcgcttcca
     4561 aggccaccgg ttaccccatc gcccgggtca ccgccaagat cgccgtgggt atgaccctcg
     4621 acgagatcgg aaacgacgtc accggcgaga cccccgcctc cttcgagccg acggtcgact
     4681 acgtcgtcat gaagatcccc cggtggccct tcgacaagtt cgtcaaggcc gacaggaccc
     4741 tcaccacctc catgaagtca acgggggagg tgatggccat cgggaggagc tacgaggagg
     4801 cgctgatgaa ggcggtccgg tccctggaga tcgacaaaga cctcggctac gccggcaagt
     4861 acgagccctg gaccgacgag gaggtgaggc acctcctcgc gaccccgacc gacgagcggc
     4921 tcttcgccat ctatgaggcc ctccggcggg gggtcaccgt tgaggagatc tcggacctga
     4981 ccgggatcca cccctacttc atcgcgagga tcgagaacgt caccgagatg gagagggaga
     5041 tcgccgccgg cctctcggtc ccggtcctcc ggagagcaaa gaggatgggg ttctccgacg
     5101 agaggatagg aaagatcctg gggaagagga gggaggagat cgccgacctc cggatcgaga
     5161 gcgggatcct accgaccttc aagatggtcg acacttgcgc cgcggagttt gcggccagga
     5221 ccccctacta ctactcctcc tacgaggacg agtgcgagct tctcccctcc gagaggaaga
     5281 aggtcctgat catcggctcc ggccccatca ggatcggtca ggggatcgag ttcgactact
     5341 ccaccgtcca cgccgtccag gccctccggg agctggggat cgaggcccac atagcaaaca
     5401 acaaccctga gacggtatcc accgactacg acacctccga taagctcttc ttcgagcccc
     5461 tgaccctcga ggacgtcatg aacatcatcg agaaggagag gcacgatggc gtcatggtcc
     5521 agttcggagg ccagacggcg atgaacctcg ccatacccct ggagagggag atcgctcgaa
     5581 gaggcctgcc gacgaagatc ctggggacga gccccgatag gatcgacatg gccgaggacc
     5641 gggagaggtt caacctcgcc ctccgggaga tggggatacc ccagccggag gcaggctatg
     5701 ccaccagccc cgaggaggcg aaggcggagg cgcggcggat cggctacccc gtcctggtga
     5761 ggccgagcta cgtcctcggc ggcagagcga tggagatcgt ctacgacgag cgggagctcg
     5821 tggtctacat gagggaggcg gtgagggtct ccccggagca tccgatcctc atcgacgact
     5881 tcctccagaa cgccgtggag atcgacgtcg acgccgtctc cgacggccac gacgtcctca
     5941 tcggggcgat catggagcat atcgaggagg cggg
//
LOCUS       DASM01000002            9048 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_93415, whole genome shotgun sequence.
ACCESSION   DASM01000002 DASM01000000
VERSION     DASM01000002.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_93415"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1643 a         2858 c         2886 g         1661 t
ORIGIN      
        1 ggatcggcgg gaccttcgat ctcgcagcga ggctcgcgaa aaaggcgcta ctacggatgc
       61 ccggcacctc ggagctcgat cggaaactct tgacggagat caaccggatc gggaccggcc
      121 ccatgggtct cgggggcgac accacagccc tgggggtgag gatcgagacc gccctctgcc
      181 acaccgcatc cctgccggtg gcggtgaacc tccagtgctg ggcgaacagg tccgcctctg
      241 cgacggtgag ggatgacgga tggaacatcg actgaagact ccgatatcga gagaggaggt
      301 ggcggccttg agggccggcg acctcgtctg gatcagcggg acggtctaca ccgcccggga
      361 ccgggtccac gccctcctcg cctccgggga aaagcttccg atcgacctcg ccggggcggt
      421 catataccac tgcggccccc tgatccggga cgggaccgtc ctctccgccg gccccaccac
      481 cagcggcagg gtcggcaggt tcacgagaga ggtcgtcggc cggggggtga ggctgatcgt
      541 cgggaagggg gggatatccg aggaggcgga ggccctccgg gggagggcgg cgtacctcgc
      601 ctaccccgga ggctgtgggg ctctggcggc ccgtcagcta tcggtgaagc ggctccacct
      661 ccccgagctc gggatggccg aggggctctg ggagctggag gcggaagggt ggggcccgat
      721 gatcgtcgcc atcgacgcct ccggtggaga cctataccgg gaggcgagat gctaccgggt
      781 gggggcctcg aagccctgaa cctcgccgcc gaggagatca gatacctcct cgaccggggc
      841 tacccctcgg ggccggcggt caggttcgtc tccgaccatc gccgcctccc ccaggaggat
      901 aggttcgtcc tctcgagggt ggcggtcccc tcggccctgg cggcggctcg gaggaagaaa
      961 tggatccccg ctgagaggct tgcgggccgg accgtcgcca tcgacggcta caacgtcctc
     1021 atcaccgtcg agagcctcct ttcaggcgcc gccgtctacc tctgcgacga cggcttcctc
     1081 cgggatgtcc gcgggatctt cagaagctac cgatcctccg aggtgacggc cccggcgatc
     1141 ggggagatcc tctcggtcct ggagggggct ggggtcgctc aggcggaggt gatcctcgac
     1201 cagcagatca gccggagcgg ggagctggcg gcgacgatcc ggggggctct cgcggacttc
     1261 ggcgtccagg gggaggcgac gacggcgagg gacgccgaca ggaggctgaa ggtcgcgccc
     1321 catccggtcg ccaccggcga cggcgccatc atcgatgccg ccctggaggc cgtcgacctc
     1381 ccggcggagg tcgcgaagag gaggggcata atccccctga tcctctgatt tttaaccaga
     1441 tccttcaaat tttagacctc gcaagctccc accttactat agttatttat tatatcctga
     1501 ccgagggtgt ataacataga tcatggcaat gtagggggta tttaagagat gaagaagatg
     1561 aaggccatcc tggcctttct tgccgttatg gttacggcgt ccctgggcgg ggcgatatgg
     1621 tttcccttca ttccgctcag cgatcactcc atggccaagt ccgtagacgc ccactggaac
     1681 ccgatatatc ggacctactc cttctccacc gaggacgacc aggccgtctc ctggatcagg
     1741 ttcgcaaggg acctcaagcc ccacgagatg gagtggcggt ggtacgcgcc caatggccag
     1801 ctctacgccc ggaccttcgc cgtcgtcccg ccgaagaact tcgccagcgg agactgggag
     1861 gggaagactt ggagcgccat ccagatccgg ggctgcgaag cctcccggct gcaggggacc
     1921 tggagggtgg atatcttcag ggactggatg aagatcaaga ccgtatcctt caacatcggc
     1981 ggccagtacg ttccttgtta gcctctgctg gctgacagcg ccgcctggga tccagagcgc
     2041 gaccaagagg caagagcttg ggctctctcg ctatagggaa gcattaaagt atatcctccc
     2101 aaatgggagg atagtttcgg atctccggca tcctttggag gagagttttc ggccggcccg
     2161 tcgagatccg ggagtggtcg accggcaggg tggaggtttt atcgatgatg atatctgtgg
     2221 ccagcggcaa gggtgggact ggaaagaccc tggtggcgac gaacctcgcg gcgtccatcg
     2281 gctcggtgga gcttttggac tgcgacgtcg aggagccgaa cagctatctc ttcttccccg
     2341 acctggagga gagagagagc tccgactgca acgtgccgat cccggtgatc gacgaggata
     2401 ggtgcaccag gtgcggcaga tgctcggagt tctgcgccta ccacgccctc gccgtatttc
     2461 caaaggaagt cctcctcttc ccggagctct gccacggctg cggagggtgc gacctcgtat
     2521 gccccgaagg agcggtctcc atggggacga gggccatcgg gaagatccac agggcgaggt
     2581 ccgggggggt gaggcttcta tggggtgagc tcttcatcgg cgagccgatg gcaacgcccc
     2641 tcataagggc ggtcaaggcc gaggcgaggg gcgacctcct cctcgtcgac tcccccccag
     2701 ggaccgcctg tcccgtcatc gaggcggtcc gggggagcga cttctgcctc ctggtcaccg
     2761 agccgacccc cttcggcctc tacgacctct cgatcgccgt ccaggtggtg acgaggatgg
     2821 agatcccctg cggggtggtg gtgaatagga gcggcatcgg agaccggggg gtctacgact
     2881 actgcgaaag agaagggatc ccgatcctcc tggagatccc catgaagagg gagatcgcag
     2941 agctcacctc ccggggggtc ctattctcca aggtgatggt cgagtggcag gagaagttcg
     3001 ttcatctcct cgaaagaatc gaggaggcgg caaaatgaag cagctggtgg tcatcagcgg
     3061 gaagggcggc acgggaaaga cgacgatgac cgcatccctc gccgccctcg ctaaaggcga
     3121 gatggtggtg gcggactgcg acgtcgacgc ccccgacctc cacctactcc tgaagcccca
     3181 ggtccgggag accttcgact tttatgggct agaggtcgct tccatcgacc ccgagaagtg
     3241 cacaaaatgc ggcaagtgcg aggaggcctg caggttttgc gccatctccg actttgccgt
     3301 cgaccccgcc gcttgcgagg ggtgccgggt ctgcaccgtc gtctgcccct cggaggcggt
     3361 cgccatgacc gatagaatct cgggccacgc cttcgtctct gatacccggt ttgggacgat
     3421 ggtccacgcc gagctatttc ctggggagga ggcgagcgga aagctggtga cgatggtgag
     3481 ggagctcgca gcccaggtgg cggagaggga gggaagagat cttgtcatca tcgacggctc
     3541 tccgggaatc ggctgccccg tcatcgcctc catcaccggg acagacctcg ccctggtgat
     3601 gacagagccc tccatctccg gcgcccacga cctggagagg atcctcgccg tcgttgagca
     3661 cttcggggtc gaggccctcg tcgccgtcaa caagcacgac ctaaaccccg atatgacaga
     3721 gaggatcggg gaactctgcc gcgacctcgg cttcgacctc gcgggaaagc tccccttcga
     3781 ccccggcgtg gtgggggcga tggtccagag gaagaccctc gtcgaggtgg aggggcccgt
     3841 cggggatgcg atccgggacc tatgggataa gatccgggcg aggctctgac gggacgcctc
     3901 cggatcagcc ccgccctccg ccccccgagg gtgggatggg gtcagagtca ggagtagagc
     3961 ctgaaggtga gggtcgagac gtcctcctct acgtcgcgat cgacccgctc cagggcggcg
     4021 aggatcgagg cggtcctgtg gaggcccgcg acgatcagcc cgccgatggg gcagagggga
     4081 tgggagagcc cctcctcgat ctggcgatcg ctggcgggag caaagaagca gcctcggatg
     4141 gtgatgacga gccgaccgtc cctctcctcg aaggaggagc ctccgatcgc ccccgaggcc
     4201 tggagggtct tcatgaagca atcgatcttc tcatccctcg tatcgaagcc cggcatgtcg
     4261 cactccctga actgctccat ctcctcccag agcctggtgc cgatggcgac ggacatgatc
     4321 atcaggcctc cggacccctg ggtcgcctgg accgcctcgg cggtggcggc gacgaaggtc
     4381 atgatgaacc tcctcaggtc ctggcagaag ggggtggcca tggagtcgtc ttccatgatc
     4441 ttccatcctc ggaaaaacgt tataaatttg ccgttccatc ctaccccggg tgttctgact
     4501 tgaacgagct cgaattcggc gggaaggtga aggaaccgga cgtgaggatg ctccgcgata
     4561 tggacgacgt cctctacgac aggaggtggg cgcgaggagc cgaggaccag gagctctact
     4621 acatgtaccg ggacctcttc ctcagcagga gggacggcga cctcttcaaa gatcagggga
     4681 tcaggtacga catcaccatc atcccgcccc ggatgctggg gcgggagtac gtcaagacgg
     4741 cgggccacta ccatccaacg gtcccgggcg aggcggtcac ctaccctgag gtctacgagg
     4801 tcctggaggg ggaggcgacc tacctcctcc agagggagga cctgagcgac gtggtggtgg
     4861 tgagggcgat ggaaggcgac aaggtggtcg tccctccggg gtacggccac atcaccataa
     4921 acgagtcgaa caagaggctg aagatggcga acttcgtcgc ccggggcttc tcgtcgatct
     4981 acgagccgat tcgagagagg ggcgggggcg cctacttcct cactgaggac gggttcgtcg
     5041 agaaccctcg atgcgaggag gcggcgcccc tccgggagat cgaggttccg aaggtgaagg
     5101 ccctcggcct ttcgaaaaat cgggagatct accccatcgg ccgggaggtg gggaagctcg
     5161 cctggctcgt ccgcccccag gattacctgg agatcttcga ggggtttctc gattgagggg
     5221 ggagaggggt tgagcggcgg cgaggagggg agaggggata agaccgcagc gggggagaag
     5281 ctgagggcct acgccgagct cctccgcccc cctcttgcgc cgatggacat cgccctcccc
     5341 ggggcggcgg ccctcctggc cgcctatgcc acgaccgggg ccctgccggc ggccttcccc
     5401 ttcgccatcg ccaccctggg ggcgtatgcc gccatcacca gctcctacgt ctacaacgac
     5461 tgctgcgacg tggacgtcga cgccgtgggg atgccggacc ggccgctgcc gtcgtcccag
     5521 gtgacgaggt cggcggccct cgcctactcg gccctcctat tcggcatagc ggccgccgtc
     5581 gccttctacc tcaaccctga gagcctcgtc gccctcatcg gagcgaccct gatcatggcc
     5641 gtctactcga gggcggcgaa gaggaccacc cccttctcct gggccctcgt cggcctctcc
     5701 ttcgggatcg tccccgtggg ggtctggctc gccatggccc cggctgggat cctcaaggat
     5761 gggcccggcc tccacctggg ggcggtgatc ctaggggcga tgatctgcat caccgactgg
     5821 gggttcacca actgtgacgc ctcccgggac gttaagggcg accggcggga ggggatcccc
     5881 accttccccg tcactttcgg catccccaag gccgcaaagc tcgtcgccgc cgcctgggtc
     5941 gtcggggtcc ttctctccat agccctgggg gtcgccaccg gccttgggcc catctacttc
     6001 gccgcggccc tggcggccgg ggtctggatg gtcggacaga ccttcgactt catccgaaat
     6061 ccgacggcgg cgagggggga gaggaccttc taccaggggg cgaactaccg ggcggtcctc
     6121 ttcgccgccc tgatcgtcga cgtcctcctc cgggcggggg tggcggggta tccgggggtc
     6181 gtcggctagg aaaacccaag aagacgaaag gctgcaagac ggcccgccgt cttcctccat
     6241 tgaattttga tcgcaaaaat cgggctttat cacttcagca cccagctcaa ccgatcgctc
     6301 gcctcttttg gcaggatgcc ccggatcggg ccgaggctgg tgaccacctc ggcgctgagc
     6361 gtgaggctcg ccctagccgc cgacgttccc gctgacattt ccaagatggc gtagactagc
     6421 tggcttccaa cgaggggatg agcccggccg gatacgaaag cccagcgggc gatgctctcg
     6481 ccgatcccgt cggcgaagat caccgggacc ttctgggtga agtcgatggt cgtctcggcc
     6541 tgggcgaggg agagaaactt gaagtcgagg ccgacggtcg ctttgaaggt gccggtcttc
     6601 tcttcagtcg tcaccttagg caataggtcg aagacgatcg ggacccccct accgtcttct
     6661 gtctccaggc tcaccatcaa ctccgcccgg cgcacaacct ttcttccttc aggcctcaag
     6721 gagaaggcga atcgtgcaag gccgtagcgg ttccctcccg atggatgggt ccacttctcg
     6781 cccattatct cctcgccatc ccaccagacc ggccttccta tgctgaccgt cggcaacttc
     6841 tcgccaggcg gcgcatagac ccctttcggc agggcgaacg cccccaccca gacggactcg
     6901 atcgggaacg cggtttcgtc gatatgcatt tttaatcctc cgtctctcct tgtgactgta
     6961 ggtcttgctg gatatcagag atcagctcct tcatctcctg aatttctgcc gccgccctat
     7021 cgccgaaggt ctcgtagtct cggagagcgg cccgggcgta gtccagcgca tcagagaatc
     7081 ggcttgctcc ggccagggta cgggcgacgt ttctgcgata catgccagcg gaatggaggt
     7141 ctcccattat ctcgcagtag cggattgcgt tgcgataata gccaagagcc tggtcgatct
     7201 cgcccgctac atcaaaaaca attccgaccg cgttatgagt cgtcgccaga tcgaatacgg
     7261 catcttgcgg taacagttcc agggccttca ggtaggactg gtgagcatcc ttgaggtggc
     7321 ggtccagttc ttctggtggc ttcttggccg cacgcgcctc atcgaagcgt ttacgggcaa
     7381 tataccctaa tgaataccag caacgtccct gtcccaacga gtttttcagc tcttccgcca
     7441 gcttcaagct aatcctgaac cagcggtctg attgatccag atcgcggatc tcgtcaacgt
     7501 ctctgtatgc attgccgagg ttgaaggcac aggctcgacc tcccgcctta tcgccgatcc
     7561 tcttaaaaac ctccagcgaa tcttcgtagg agttcacgca ctctggttta ccttgctcgc
     7621 gctggatctc gcccagctcg tggagggata cggccagagt ccttatgagg ctccgatcat
     7681 aatcgcccag ctcctccggc gattttatca gggcgtcggc ggctcgctgg cggtcaaagc
     7741 ccacacgagc gctttggagc cgcatcgcct cgtcccactg gcgctcttcc tcggcaagca
     7801 gaacccgata ttctgtcacc aggctccaca gctcctcaaa gatagggata gggccgtcgg
     7861 tctcggcgtc gacaaaataa ggcacgatct cattgacgag cctcctccac tccgccctcc
     7921 ggccagtatg acggtagagc tgacggagcc cctgcatggg gccgattacc tgacgcagcc
     7981 aaccgcgttg aagcgccagc ctcctggcgt ggaggaggtt cgtctcctct gccgtcaggg
     8041 cggcgatcac ggccacgttg ccgccttcgt actggctcaa gtagtaatcg cccaataatc
     8101 ccacggcatc cacgaaggct cgggaagccc cctcgtccga tggataacgt tcctcgaaga
     8161 ggttcttgaa gtaccagggg agggctgggt ggaccgagta atatcctcca ccgtaagccc
     8221 tgagcagccc gacctccgcg gcccggtcga ggatcgagat ccagcgatcc ctggttaggc
     8281 cgcggacctc ggatagagac cattcaagtt ctgggttgcc catccactgc aagacatcgg
     8341 cgtccacaaa cccctggaag aggtggagga gagcgagccg tttcagctca tcttcatcga
     8401 aggcgtgctc aaagccgtac cgtagggagg cgcccaggga cttggtgcgc ccctccctct
     8461 cgtcatcttc gatctcggcg gtgcccgcct gaagcttcga gacgaacttc tcgatatctt
     8521 ctttcgattc gaggccgtcc cggagcgcct ggcccaccag caccgttatc gtcaaggggt
     8581 ttccctgggt gaacttcaga agcgccatcc agtcttccac gtccatgaac cttttaccgc
     8641 gcctatccgc cacagcccga gccagctcca ccctctcctg catcggcatc ggcggaactt
     8701 ctatccgaac cggcaggtct ccgagccatc cggactcgtc ccgtcgggac gtgagcagga
     8761 acttggcccc ggtatcccga gcatcccgga ggaagtcgga gagctccttc tgctcatcag
     8821 cgctccaggt cgactttgat cccgttggaa atccagcgac cgattccacg ttgtcccaga
     8881 tccagaggac gggacatatt ttcaggatct gaatagctac atctcgcctc tcttcgtcgt
     8941 ttaaggtcag ccagtggatg cctccacctt ccagcatacc gccgaagacg tccccgatct
     9001 tgtccaggac gcgggccagg gggttgtgcc gttcaaagga ggtgaaga
//
LOCUS       DASM01000003           18116 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_94154, whole genome shotgun sequence.
ACCESSION   DASM01000003 DASM01000000
VERSION     DASM01000003.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 18116)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18116)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18116
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_94154"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    17549..17730
                     /estimated_length=182
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    17895..17948
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3668 a         5376 c         5482 g         3352 t
ORIGIN      
        1 ccttgaaatc aatgattttg atgtcagcca ccttcacggt cttcccggtt ctcctgatca
       61 ggctcgagta ctggagcagg acgcatcggg agatgacggc gaaagcctca agtttcctca
      121 tcatgccctt caggaggttg aaggattgaa aaagatagcc catttgaacg gcgcattcgt
      181 cctcatcgcc ctggccctcg cctcgatagg cccggcctgg gcctctgaga actacatccc
      241 ttcggtatct ctcgacaact actggacgac cagcggatct ccccagctcg acgcgagcct
      301 ggcggggacg aacgagtttg gccggggcga gaccgtaacc ctcttcgtcg acatcaccaa
      361 ccgcggtagg atcatggggt tcaaggccga caagaaggcc gagaccaaga tggagcgggc
      421 tctggcagac cgggagctcg actatgagcg ggagaggacg acggccttgg gcataaccgg
      481 gaccctcagg tccgagacca accaagtcga ggtcaaatcc ggcgaccagg tggtagaggc
      541 cctccggtcc ggcgagaagt cgagggagcc gttgaggttc accatcaaga tatcgaaccg
      601 ggctcccgcg ggggagtatc ccctccgcct agagctcgtc tacgactatc agttcaacgc
      661 tgaggtggac gccaccgacc tcgaccccga cgtcggcctc cgagggttcc agacatctta
      721 ctggtaccag cgggccaacg agaccgtcac catacccgtg atcgtcaaag aggaggctta
      781 cttccagatc accgaggccg aagccgacct ccgctccggc gagaagggcg gtttgatcga
      841 ggtgacctac aggaacaccg gcgagaaggt ggccgaggac gccatcacca ggctatctgt
      901 cttcaagccc ttctcctcct ccgacgatca ggcctacata ggaaccctcg gccccggcga
      961 ggagagggcc gtcaggttca agatcgacgt cgactccgat gccaccccca aaccctacag
     1021 catcaacagc gagatcaaat acacagacca ccggggaggg accgtcatat ccgagagcat
     1081 gaagatcccc gtcgaggtta ggcccgcccg gaggtcttac acccttccag cggtcgccgt
     1141 cctgatcctc ctggctggcg gaggggcaac cctctatcgg aggaggaagg ggggatgagg
     1201 ggccctcccc tccaccgggg cgtgggaaat tgttaaatag tttttcaaga gaataaggat
     1261 ttataagcga tgaagggggg accgtgccac cgtctgatcg ctcgagggga tcggctctga
     1321 tgaggaacga tgggggatga tagccccgga tcgtccagag ctggcgacgg ttgatggggg
     1381 aggccccatc cgcgcggtcc tcaaggtgag ggagaagcat gaaaaaggtt ctttcgttgg
     1441 gtttgatctg ccttttgagc ttatctttta gctcggcgat cttttcatct gccgtggccc
     1501 aggccgatcc tgggagcacg ggcaggatga cgattcagta ccttctcgaa gatcccgcga
     1561 tccagagggg cgccgacacc ctgacgaccg ccgacgggtc cgtcgaggtc tacgactcgg
     1621 tgactatccc cgggcttcac caccggatcg tccccgggga gccgatcctc cccttcagga
     1681 ccgccaggat actgataccc tacggccagg aggtctcgga ggtgaaggtg ttccccggaa
     1741 acgagaggta tctgggcaga gtctcgataa ggcccggcca gacctccgtc cccattggat
     1801 tcaccaggaa ctgtaccagc tgccatccag aggtcaactg gacaaagacc ccggacctcg
     1861 ccttcaggga cgaggagatc tacgggtccg agagcccata tcccggggag ccctcctcga
     1921 tcctgggggt tcagaagaag aacggctacc agatcctcta cgtaaacctc tacccgatca
     1981 ggtacatccc caagacgggc gaagcttaca gctatggggc cttcgacgtc gaggtgacga
     2041 ccgcccccgc cgagaccctg gacctgggga tgtatagagg cctcttggag gatcgggatc
     2101 aggtcagaaa gatcgtcgac aaccccgaga agacggggac ctacgcccca gacctggcga
     2161 tgacgggaaa gtcccttctt ctggaaggag attacgatta cgtcatcatc accaaccagt
     2221 acctcaaaga cgccccggga ccctacaact tccaggccct ggccgaccat aagaaaaaga
     2281 agggtctaaa cgcggcgatc gtcaccgtgg aggagatcta cgccgcctac aagtacacct
     2341 accataggga cgatcaggag atgatcagaa acttcatcaa ggacgcttat aagcacaacg
     2401 gcataaaatt cgtcctcctc ggcggtgacg gtgatggggc gagggtcggc ggggagacct
     2461 gggacgccat cgttccagca aggctccttt atgcctgggc ttacgagccc gatcccgact
     2521 gctgcgccgt cccaaaagag aaggtgggga tagcatccga cgtctactac gcctctcttg
     2581 agggaacctt cgacgggaac aacaacggga tatacggcga gccgaccgac ggcgtcaact
     2641 tccaggcgga cgtctacgta ggccgggcgc cggtggacaa ctacgctgag ctctccaact
     2701 tcgtccggaa gacgatcgcc tacgagtcga cccggggcga cgatccgtat ctgacggcca
     2761 tctggctcgt tggcgagtac ctggagccga tggcctacct gacgactaac ctgagcgaga
     2821 tcgaggatca tgcccctgag gagcagggct gcggtgacgg tggctgtggc ggcggtggct
     2881 gtggcagcgg cggctgcggc ggagagggtg gctgtctcga tctgtggggt ggcgactaca
     2941 aagaccagat taaggagatc atacccgagg agttcaacat ctggaccctc tacgatcggg
     3001 actatcgcgg cggcgactgt gaacctgacc agcataactg gcctcgatcc ctcctgatag
     3061 agaagatcga tgccaatatg atccacgcca taaaccacgt cggaacggcg aacttctgcg
     3121 acatcgtcgc ctacgtcatg aagatgggtt acaaccacgc cgacgccctc aataacagca
     3181 agtacttctt gggatactcc caggcggggt atgcggcctc cttcgacaac agggacccgg
     3241 aggcggttta cctcccccac gactccgtcc tcgagcactt cgtcacagct ccgggagggg
     3301 ccttcgcctt catagggaac agcaggtacg gcctcatcga cctcaacagc atcaacgaca
     3361 gcccatccca gaagttcgac aaggcgttct gggaggagat cttcaaccca tcgtatacaa
     3421 atgggaacag gagcatcggc cttgtccacc agatctccaa ggagagcttg attggagggg
     3481 ttttgggtac cgacgagatg aggtactgct actacagcat caacctcctc ggcgatcccg
     3541 agaccccctt cctcttccca ggggccgttc cggctgcagt ggccgaacct ccggtgccgg
     3601 aagccgatcc tggcgcagcg gtgaagagga gcgactcgag ccccgagatc ccctccatcc
     3661 tcagcgggga gatgagattc gagttcggag agcctatgga gttcaaattc ggcgagggga
     3721 tgggcatgcc ggggatcaag cccctctgac ctcttttttc gtccctgggc tggaggtctc
     3781 ctccggcccc catccacctc aatcacctca aaaagtaggc ctctttcctg cggtgacctt
     3841 tcctcgaacg ccaaccttcc tcgggaggaa gacgccggga tgggccacca gggcaggggt
     3901 cttgtggcag atcccccccg accttcgcct cggtgatgag catgacgagg gggcccagcc
     3961 cccgggggtc ggctgggggg ccgtgggaga ggacgatgta aacgagggcc aaaaggtcga
     4021 tccccacctt ccggtaaata taccattcaa cgggggcagc gccgtctttc gttccgggga
     4081 tgaaggtaca aaaatccaaa aggtttatta aatcagagga gaaagatatg ggccaggagg
     4141 tgagcagatt ggtcaagtta gctccattgg atcttctggc catagtgctg gtggtagtcg
     4201 gcggcctgaa ctgggggctt tacgccttcg gttttaacct ggtgagcatc cttctgggat
     4261 ggatgcccat cctcgagacg gtcgtctacg tcttggtggc cctggcggcg atatacctcg
     4321 cggtcctgac ccccaggctc tccaagtagg gaagaagaat aagaagtctg ggaggcgcgg
     4381 ttctcgcgcc ccaccccaga cgttcctccg cgggttccgc ctcttctcat gatcgcgatc
     4441 ttcccaatag ccgagagatc gtctcaggcc ttcagagaag gttttgcgcc agggcacccc
     4501 caacttcaat gatccccaaa agctccatcg agactatcag gatcggctgg tggaggaggt
     4561 agatcggaag ggagcatctc cccagggtgc agatccctct tgccggcgat ctgggggaga
     4621 tatccgggag ggggcatctc ctctcgcctc tggggtaaag gaggcctcct aagaagatcc
     4681 ccaccaggac gacgccggac caggggagga tgggaaagta gtcgacggaa taaaagccag
     4741 aaggtacgag gcccaggggc aaaagccatg gggaggctac ggcctggccc cggaggatta
     4801 ggcctacggc gatggccccc acccccaggg ggagggcgag acggggcctg tcggcgaggg
     4861 ggatggagag gattatggtg atcccgatga ggtggaggac gccgaagacg acgaacccct
     4921 cccccaggag gaggagggtg gagagggtca cgcctatccc ccaggagaag atcccgatcc
     4981 cccggcggag gagcctcaaa ccgcccccga ccctggcggt actgatcgcg agggagacgc
     5041 cgactaggag gaggaagatc gaggccgtcg cctcggcgat caggggcatc cccccggatc
     5101 ggaggtcgac gacccggggc cccgaaaaga aggcgaggtc gtagagggcg tgaaagccca
     5161 ccatcatcaa taccgcgaac ccccggagga ggtcgatctc ccagtaccgg atcaaggctg
     5221 gcataaagac cttctccctc atcatcggcg aagattcgcc gtatggtcgt cgagagatcg
     5281 aggagagaga ggtccagcaa atcggttccg gcgatagaag agagggaggg gcggtccctc
     5341 agggcggcat ccggccggga cccgccagga acctgccgtc gcgaccctcc cctttgatgt
     5401 cgccagcttt gtagtcattg acgaaggtgt atagaggcca tcccctgtag gtcgtctggg
     5461 ccaggccatc ctcccgataa atggtgccga agtcgaagta gctcaggttc gggggtatca
     5521 ccacctgacc cgtataaaag ggcggccagt cgtggatgca ggtttcgccg cagctgctag
     5581 ttccgctccc tgggacgtcc agagagaagt agtatagggc gtatccatcc tggtccacca
     5641 ggtactcctc taggccttcc ggtgaggcga ggctgatggt gttggcaggg gctgcggccc
     5701 ccgtggcgac gatggcaaaa gacgccaccg ccagggccag cacggctggg atcttcaatt
     5761 ttatcaccga ccccttatcc tcccgctcaa cgtttaaagg ttccgagtgg aggggatgaa
     5821 aaaatggtcc cgcaaggccc cctcatcccg aggaggcttc agggggcgac gacggaccag
     5881 actcctccga ggccgtgccc caggacgtcg ccggggacct tgtcctcgaa gtaccggtag
     5941 aggggcattc ccttgtaggt ggtctggaag gagccatctt ctcgggggat cccgttgaag
     6001 tcggtcacgt tcagctccgg gggaacctca atctccggga cgaagaaggg aggccaccgc
     6061 tcagcgcagt cgtcgatgca ggcgctgacg ttgccgccag gaagatcgtt ggcgaagctg
     6121 tatagggcca tcccatctcc gtccacgagt aacgccccca gggctccatc ggtcgatatc
     6181 ttcacggtat aggaggtttc tggggcttca tctcccaagg ccgagcccgc gacgaccgtc
     6241 gccagcagag ccaatgccag aagggttttg gatatcaaat tttcacctct ggactttatt
     6301 tgcgcggcct ggatattaag atttttccaa gatttggggg ttgactaggg gctcagccgt
     6361 ccttcggcgt ataacccggg tccctctcgg cctcgacccc cccgggcgag tcgaagtgct
     6421 cctcgtaggg cttgatgtcg aggatggggc tcctatcgag ggcgtcgagg ccggtgacgg
     6481 tgatcttcct cccctcgacg ttcaggacct ccaccaccgt gaggccgatg gggtttggcc
     6541 tcctggggga tctggtggcg aagagccccc tcttggggat cgactcgtcg cccatgggat
     6601 ggacgaggag gtcgtagccg agggcgcggt ggatgtagaa gatgacgaag atgtgcctgt
     6661 acctctcgat gccctggagg gcttccagat acctctcgtc gatctcgagg gtggagaggg
     6721 agtttctcat cgtcttgggg tcctccggct cgccgaagtc gcagtggacg acgcctatcg
     6781 gggtgaaggt gacgggctcc aggttcatat cgtatcaggt tccgtagtct tccattatca
     6841 gattttctct cctttattgc aaaggggtcg atctgaacgg acaggaccga ccccggtcct
     6901 cacgcctggg agaagacgga gggggtggcg gatacgtcgt agaaggtccg ggagagcttc
     6961 tccgggtaga cgacgacgag ctttggaacc tcgctcccga ggtgtcttgc gtcgtagagg
     7021 tagtagccgc tcttgaactc ccccgccccc cgggacacca ggaccgcccg gtggggctcg
     7081 gtcttcctca ccgagaggtt gccgaagcct gagaagaggt ccaagagctc cggctcgatg
     7141 tgcttggttc ccagggcgag gacgtagagc ctggcgaagg ggtcgaggcc gaacctgaca
     7201 tcgaatactg cgtcgccatc ttcgagggac atggtcacct cccggacctg gatgtactcc
     7261 ctcccccggt cggctaaggc cggggaggtg aagaggatga ccaccagaag aaatgccgcc
     7321 ctcgatctca taacctctca accttggggc gccttgagaa tgacggggac gatgagcatc
     7381 aggatcaaaa ggcctatgaa gacgaggaag aagagggtct gccgtcgctg ggcggatctg
     7441 tacttcgact cgcagacctc gtcgcagaag gtctccgcat catcgatggt cttgccgcag
     7501 acgatgcagt gcttgtgaga tggcgctttt gtcaacccga tcaccaacgt cgaccctatt
     7561 ggctggagcg tagataagat ttttggacga cggaggggct cggcgacaga attgatggaa
     7621 agacgtatta tggattggag ggatctcgcc tctggtggac gtatgctgag attgacctta
     7681 gcgatcttga tagccctctc ttccggcctc ctccttgggg ccgaagctag gatgtacatc
     7741 gtcgacgacg acggctttgc ccagtaccgc accattggcg aggccatcgg cgtagccaag
     7801 gatggcgata cgatatatgt gaaggccgga acttaccggg agcacatcac cttgaacaac
     7861 aggatcatat tgatgccccc ccggggggag gaaggggccg tcaacctcgt cggcgatgga
     7921 aacgacatcg ggatcgaggt cctcgccgac ggctgcagga ttgaggggct caccatatcc
     7981 gacttcgcgg ggccggggat ctacgtcaat tcccggggaa acgagatcgt aaagaacgtc
     8041 ctcgtggata acgtccacgg aatctatctg aaaggcacct ccgagaacct cctcgagaac
     8101 aaccgagagg tgggggggta ctgcgggatc gtcctcctct cctccaacaa caacataatc
     8161 aaggggaacc tcgccacggg ttgcacgggg tcgacggagt atcccggcgc ggggatcctc
     8221 ctcaactcct cctcgaagaa ctccatctcg gggtccgagg ccgagggctg ctcccggggg
     8281 ttccacctcg tcctggggag ctcggagaat gagttagcag gagcgaaggt caatagctgc
     8341 aggtatgggg tcctcgtcga gggcggctcc tccgccaacg cgttccggga ggggcacttc
     8401 gataacgtca ccaccgcctt cgccctcaac tccgccgcga agaacctgat cgagaagaac
     8461 accatctcga actctacgaa cggaatcgtc gtcgccacct cttcggagaa cgtggtggca
     8521 gaaaaccgcc tctcagacct cgacctcgca attatgatct cgttggggtc tacggccaac
     8581 gtcctcacca ctaatcacgt cgaaagggcc aactcgggga tcgtgatcac cgaatctacg
     8641 gctaacagct tcgacgaaaa ccgcctcgta ggggtccagc tcggcctctc cgtcgatgga
     8701 tcgggggctg agagcttcga taacgcgatc cctgaatcga acatcatcga cggcgatcct
     8761 atcctctacc tctacggccg atccggcgag gtggtggcgg atcggaagtt aggccacatc
     8821 accctcgcct cctgcgataa ctgtactgta gagggttgca tcgtcaccaa cgacgcccta
     8881 ttcacccact catctaaggg gtgcaggata gtggagaacg tcgtctccaa agcctatggg
     8941 atgctgatcc ggagatccga cggcaacgag ttcaggggca accaggtctc tgagagcagg
     9001 gtcggcggga tcatgctggt ggagtcaggg gggaacgtca tcaaggagaa caacctctcc
     9061 aggaacggct gggacggtct cctcctccgt agatcggaga agaaccgggt cctatccaat
     9121 accgccgagg ctaaccagca ggctgggatc aggctcgacg gatccaacga cgcggagatc
     9181 gccgaaaact ccgtcatcgg aaacggcgtc ggcatctcca tcagcgggtc ggagggctgc
     9241 attgtctaca ggaacaacct gatcgaaaac tccgtccagg cggaggacga cggggagaac
     9301 ctctgggact ggggagccct gaagggcgga aactactggt ctgatcaccc ctgcgacggc
     9361 agcccttgcc ttgcccaggc gaggcccgtt ggggtcaatg cctccgactt ctaccccttc
     9421 ggagagaggg atggatgggc atgagagccg aaatgtactt gaaggggtcg gtggaatagg
     9481 aggacctcat ggctaagaag aagtctgagg gcagcggact gatgtcctct gccggtctca
     9541 tgaggtattt cgacgcggac gatacggcga tcaagctcaa ccccaagacg gtgatcgcca
     9601 tcagcacggg ggtgggggcc gcggttatgg ctctgaactt ctccttcggg ctgtggcctt
     9661 gaaggggccc tggccatccc cctgacgacc atttataggc ccgccgtata aggccggttt
     9721 tgagccctac cgcctcaatc ccggccatgg cccaaaggcc gttctcctcc ctttatgcct
     9781 cttcggtgag atcgccggtt ttgatcagac cgccctcttc tcctcgcgga tctggccgag
     9841 gatcccgtca acgaccctct caaagctctc ctttgcggcg gtatcccggt cgacgaaggc
     9901 caacccctca tcccccaggg gggtgatctc cgggtccagg ggtatcgccc ccaggaaggg
     9961 gacggagagc tcctctgctg ccctctctcc gccgccggtc ttgaagatat cgatcacctc
    10021 tccacagtga ggacagctga ggccgctcat gttctcgacg attccgagga gggggacccc
    10081 catcgccttg accatgttca ccgccttcct ggagtcgagg agggcgacct cctggggggt
    10141 ggtgacgatg acggcgccgg tgagatccgg caggagctgg acgacgctga ggggctcgtc
    10201 gctcgtcccg ggggggaggt cgatgatgag gaagtcgagg tcgccccagg agacatcggc
    10261 gaggaactgc tttatcgccg ccatcttcat cggcccccgc cagacgacgg cggagcttgg
    10321 gtccgagagg gcgaaggcca tcgagatcac cttcaggccc ttcgagtcga cgggctcgat
    10381 ccccccgggc ccggcgagga gcttctccct ctcgacgccc aggagcttcg ccgtgctggg
    10441 gccggtgatg tcggcgtcga ggaccccgac ccggaacccc cgggcctgga ggacccgggc
    10501 gaggttgacg gcgacggtgc tcttcccgac cccgcccttg ccgctcgcga tcatgatctt
    10561 gtgcttcacc ctccccatcc tcgcagagac ggcccggagc tctacatcca tctccttcct
    10621 cgggtctgtg caggttcctc tggtggcgca ggcttcgcat ttgctgtcgc attcttccat
    10681 agtaacacca tcatgaattt atcttgaggt tcatcggcat agtttcggca tcctcttatt
    10741 cttcaaatcg gaggcgctat catcgtatca ggggtcgaag cgaccggatc gggttgagct
    10801 ggaccttcgt atccctcgaa gccgtgatcg gggtcggacc tgatggcgat ggccccggcg
    10861 gcggatcaga tctcccctcg cccctcccta gcccggaagt agaggcggcc ttcgtgccgt
    10921 agccgtctga tctcatcctc cgcctccagc acccccaggt acttcaatat ctcgtttcgg
    10981 tggagccccg agagggacga gagctcctcg agggtgcagg gcctccgggt gaggaggtcg
    11041 cggatcagaa cctcggtcgt cttcgagagg tctctccccc agtcggcgat cacctcggcg
    11101 ccggagaaga gctccagcat ctcctccatc tcctcggggg agaggggctg gaccggctcc
    11161 gccggaggcc tcaccaccgt attgagctgg accctatcgg cgccgatcct ctcggccgcc
    11221 tcggcgatga ggggcgcctc gccgtcgttg atccccttga cgagcatcac ctccagccat
    11281 atctcgccag agaactcctc cctgaggcgg acgagcccct cgaggagatc gtcgatcgat
    11341 agttccgggt gggggcggtt tatcctcttg aaggtcgggg ccgtcgcggc gtccagggag
    11401 gggatgatca ggtccgcctc cagcacctcc cggcggacct cctcgcggaa gaagagggtc
    11461 ccgttcgtca gcagggcgac gggtttatcg accgcctcct tcgctccccg gatgatctcg
    11521 tcgaggcccc ggtggaggag gggctccccg gagccagcga gggtgaggta gtcgaactcc
    11581 accgcctccg ccgcgcccgc ccccccatct ccatgcccaa cccgatccgg cttctcctcg
    11641 gatagggacc ggatctcctc caggacctcg gcggtggaga cgcgctcctc ccgggcgagg
    11701 gtgaggcagg tagtcggtcc cagctggcag tagacgcagt cgagggtgca ggtcttgaac
    11761 ggcagaaggt cgacccccag ggagaggccg agccgccggg aggggacggg gccgaagagg
    11821 tgcttcatgg ttcacttcct gaggaatcgg tcctcgattc aaaggttaaa gagatgatgt
    11881 actaataggt tgcgtcaaag gacctcaagc ctgggttcct ggaaaggcga tggataagtt
    11941 ttttctgttc tgcccagggg cctatttcaa gatggcatcg ccttgggggg gatgggcctc
    12001 ctcgacggca ccttcggtcc agaacggatc tataggatgc cctagatctc gcgccgatca
    12061 ggtctgggcg atcgactgaa aggacgggtg gggcgggcgg caggatgccg gagggacagg
    12121 atggagcgat atccccaggc ccaagcccct cccgctccca aaaggattaa agaggctata
    12181 tcagccgatc tcgacggccc tcttcgatat ctgccatatc cggtcgatgg tgaccatatc
    12241 gggatcggat ccccggaagg cgttcttgaa attgaccgca atgacaacga tatcctcggc
    12301 ccctccgata tcagcggcgt aaagataggc ctcatcaccg accttcggct ctggaggtac
    12361 gacgatcccg gagccgacga tctcgccgtc ctttgaaagc tggaggtaga cctttttgcc
    12421 gtccagatcc acggccccga tcgagaggtt gtatccttcc atgagatcga gggggtggtc
    12481 ggtggttatc acagcatcat catttatctc gaagaccttc tgagaaggct gatcctcggg
    12541 gcgatatctg accagatcga cggtggcagc atcgaattct gctcctcgga aggcgttctt
    12601 gaagcggacg gatatctgcc catccccccc tgacccacca tcagtgtaga cgtaggtctc
    12661 gtctccaacc ctcggtccag gggggatgac gacgatggca gagtcgatga tctcgccgtc
    12721 ctttgcgagc tggacgtaga ccttatcccc ggcgaggtct atcgccgcga cctcaagccc
    12781 gtacccctcc atgagattga ggggatggag ggtgattacc tcggcaaagc cccatatctc
    12841 gcctttcttc gcagaagggc catctccggg aggcggaacg atcatctcga ccgtggccag
    12901 atccaaggaa gctccccgga aggcgttctt taattggacg gcgatctcca ccccaccttc
    12961 agagccatcg ggggagtagg agtagatatc gccatcaatc gggaacctcg gagggatgac
    13021 gatggcagag tcgacgatct ccccgtcttt tgagagccgg agatagacct tatccccgtc
    13081 aaggtctatc gccgcgatct cgaactcgta cccttcctcg agagctaggg tctggccggt
    13141 gatcatcagg gtatcctccg atatctctaa gatcttctcc gagggtcgac cttccgacca
    13201 ctgaacgatc tggtcgacgg tggccaggtc gattgctgat ccccggaagg cgttcttgaa
    13261 gcggacggcg atctctcccc caccctccgg gccatcgggg gcgtagaggt atgtctcgtc
    13321 cccagccttc gcttctggtg gtatcacgat ggcagagtcg acgatctccc cgtccttggt
    13381 gaggagaaga tacgccttat ttccatcgag gtcgatctgc ctcagccata gcgcatatcc
    13441 ctccttcagt ttcaggggtc ggcctatgga tatcaccacc tcgtcggtgg tctcggtgat
    13501 cttctccgaa ggcctacctt ccgagcgctg gacgatcgag tcgacggtgg caaggtcggc
    13561 ttcagctccc cggaatgtgt tcttgaagtg gacggcgatc tgctccgatt cgcctgagcc
    13621 atgaggagcg tagatgtagg tcccgtcgga ggatttcgct tctggcggca tcacgatggc
    13681 gtagtcgacg atctcgtcgt ccctggtgag gaggactaag gccttattcc cgtcaaggtc
    13741 aaccgcttcg atccatagac tgtacccctc cataagtctg aggggctcgc ctgaagatat
    13801 cacaatcctc tcggtcgctt catagatctt ctctggaggc ctcccttctg acctttgaaa
    13861 aatagagtcg acggtgacta ggtcgaagtc ggcccctcta aaggcgttct tgaatcgtac
    13921 ggcgatctgc tgggattccc ctgagccatg gggatcgtag atgtacgtcg cggcgaccat
    13981 ctttgattcc ggaggcatga cgatggcgga gtcgacgacc tcgccgttac tcgatagctg
    14041 gacgtagacc ttatccccgt cgaggtctgt tgagatgaca gccagctcgt acccttcctc
    14101 cagctggagg ggttcaccgg tggcgaggac catctcaccg aggctgttgt agatcaccct
    14161 cgatagctga cggcttttca taagatctgg ctcctttgag gagttgtata gtaggccgtc
    14221 ttcatagccg ccgaagagag cctcgccgag gcagcttaca gacctaaaat gcccccagcc
    14281 ttcgatctga aagggctcga tctggccggc agaggctccg tcgcatccgt cagatgatag
    14341 gtcgcttcca ccaacgacaa ccgcaaagat cgccaaggcc accagcacca tagggtccaa
    14401 gatcgctttc attatttctt acaccctccc tggtaatgga gggctgcatt aatgggcgaa
    14461 gaatctttcg atgtaatctc tcaatcccgc ctctaagaga tcaacccgac catggtgaac
    14521 taaggagttg tcggttcgat gctccttaaa aggctgtaat cgatatctac attagaatta
    14581 agtttataac caaaatggag ttcctccccc ttcttagtcc aagttccatc cctgctccga
    14641 cgagtcctag cgttctctct tcggggtttc tttgaacttc ctggaccggc ctctataaac
    14701 gtcgcatcct gaatggttcc aatggggcca cggcttttaa gccatgcata gatatagcgg
    14761 caccaataat tcttggttct cgaagcatct ccctcggccg cccccaaacc cccggccccc
    14821 tccatgagcc gccggaccgg gcctcggccc ttaccacctc ttcaagcgaa ccgttcttca
    14881 ccgggccgcg gtcagaagca gcagaacccc cgccgccagg gtgatcccca gggtcagccg
    14941 ccggaaggag gcctcgtcca ccctcccgaa ggcgcggccc ccggcgatga accccaggac
    15001 catcgccggg aggaagaaga ggctattatt gagcatctct ttgttgatga ggcctgccca
    15061 gaggaagtag gcgaaggcga agaggtcgac ggccgtcagg aataggacga tgagcctcct
    15121 gaactccccc ttctcaaagc cttggccccg gaggaggagg gccatcggcg gccctggaag
    15181 gccggtggag ccgcagagaa accccgccag gaggcctgag gccgccgagg cgattccgcc
    15241 cctccggagc ttcggcccca gatcccggag gaggaggatc gagaagacga tgagggcgat
    15301 ccccatccct cccttgagga gccggtcgtt cgccgccatc aggacctcgg cgccgatggg
    15361 aacgcctgcg agccccgcca acaggagggg ccacgccccc gcgaggtcgc cccccagcct
    15421 ccgcccctcc cttccgagga tcatgaggtc gatcaggacc tcgtagacca tgatgaaggg
    15481 gacggcgtac ttcgccccca ggaggatcga gaggaggggt acggccagga gggcggagcc
    15541 gaagccggtc agggtcctga cgagcatggc ggcgaagatg atgagaaaga tcgggatccc
    15601 tgggaacggg tcgatcatgg tccagtggat aattgtattg gcccatttaa tcctatcagc
    15661 acaaggagga taaacgctga gatcacagaa gaccttcctc tgaacatctt cggattcatc
    15721 agccataacc tctggccaca ttccaagacc ctaccatccc aggccctatt cattgatgct
    15781 gactcaggtc tcagctatct ttctatcatg gcagggattt ctggagtacc gaacctgtgt
    15841 agttccgaac cgacaaagcc taaataggac gttatcaatg ccctacccgt tgttggagga
    15901 ctaggatgat ctctgaggtg gcaggacaat acgacgcaaa agaggtagag ggaagggtcc
    15961 ggaagttctg ggaagatcgc ggcatctacg ccctggccag aaccacgagg gccggcggga
    16021 gaaagttctt cttcgtcgac gggccgccct acaccaccgg gaggatccac ctcgggaccg
    16081 cctggaacaa gatcatcaag gactccatcc tccgctacaa gtcgatgaac ggcttcgaga
    16141 tcaaggaccg ggcgggctgg gacatgcacg gcctccccat cgaggtgaag gtggaggagc
    16201 acctcggctt caagagcaag aaggagatcg agatctacgg cgtcgaccgg ttcatcgaca
    16261 agtgcaagga gttcgccctc agccagaagg accagatgac agagcagttc aggaccctgg
    16321 gggcctggct cgactgggag gacccctaca tgaccctcaa ggacgagtac ctggaggcag
    16381 cgtggtggac cctcaagaag gcgaacgaac gggggcttct ggagcggggg ttccgggtag
    16441 tcaactggtg cccccgctgc gagaccgcca tcgccgacgc cgaggtggag tactgggagg
    16501 agaccgaccc ctccatctac gtcaagtttc ctgtcgtcgg ggaagagaag accttcatga
    16561 tgatctggac gacgacgccg tggacgatac ccgccaacgt cggcgtcgcc gtccaccccg
    16621 acttcgagta ctccagggtc cgggcctgga aggacgaccc cgacggggcc gaggtcctga
    16681 tcatagcttc gcccctcgtc gagggcgtcc tccgggaggg gcggtataag gggtacgagg
    16741 tcctggaccg gttaggaggg gcggagcttg aggggctcga atacgaccac cccctcgccg
    16801 acctgatccc cgcccagagg gatatcatcc acaagataca cctcgccgac ttcgtcaccg
    16861 ccgagaanna cttcgagctg ggggtcgccg agggtatgcc catcttctgc cctgtggggg
    16921 aggacggccg ctacaccgcc gaggccgggg agaagtacgc cggaaagtac gtccggtccg
    16981 caaacgagga ggtgaacgag gacctcgccg ggcggggcct cctcctcgcc gaaggggaga
    17041 tctcccacag gtacggccac tgctggaggt gcaagacccc catcatcttc atcgccaccc
    17101 ctcagtggtt cctgaagatc tcggacctcc gggaggatat gctccaggag atagcgaaag
    17161 tgaagtggta ccccgactgg gcgggctccg cccggttcag ggactggatc agtggcgccc
    17221 gggactggtg catctcaagg cagaggtact gggggatacc ccttcccatc tggatctgcg
    17281 agacctgcgg cgagatggag gtgatcggct cgatggagga gctccaccag aggagcggct
    17341 ccgtggtcag cgagctccac cgccccgccg tcgatctggt gacgataaaa tgcccctgcg
    17401 gcggcgagat gaggagggtc cccgacgttt tcgacgtctg gtttgacagc gccgtcgcct
    17461 cctgggcgac cctcaactat ccccgtgaca gaaggcagct ggaagagtgg tggcccgccg
    17521 acttcatcac cgaggggcac gaccagacnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgaacatcct ctggaacgcc tatcgtttcc
    17761 ccctgcccta catggtcctc gacggcttcg atcccatgag ggcgaccctg gagagccgcc
    17821 tcggcgacct gaggcccgag gaccggtgga tcctatcgag ggcgaactcc ctggcggcgg
    17881 aggtagaggc ggggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnga cctctcccgg tggtacatcc agctggtccg cccccggacc tggatcgaga
    18001 ccgacgaccc cgacaagctc gccgcctacg ccaccatcat ggaggtgatg gcgaccctgg
    18061 cgaagctcct cgcccccttc gcccccttca ccgccgagac gatctaccag aacctg
//
LOCUS       DASM01000004            3688 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_110176, whole genome shotgun sequence.
ACCESSION   DASM01000004 DASM01000000
VERSION     DASM01000004.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_110176"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    3391..3410
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          847 a          933 c         1111 g          777 t
ORIGIN      
        1 gccatcttcg ccgagaccgc cgacaacgag aaggagcacg caaaaaggct cttcaagttc
       61 ctggtggagg gcggcgaggc cgacgtccgg gcgtccttcc ccgccggggt cataggtaag
      121 accgtcgaca acctccggga cgccgccgcc ggggagaagc acgagtactc ggagatgtac
      181 ccggagtttg cggcaaaagc cgatgctgag ggcttccccg aggtcgccga gacgatgaga
      241 aatatcgccg ccgccgaggt cggccacgag aagaggttcc tcgccctggc gaagaacatc
      301 gacgacggga cggtcttcaa gcgcgagggg gtcgtcagct ggaagtgcag gaactgcggc
      361 tacatccacg agggggccga agcgccggag gagtgccccg cctgcgacca cgagagggcc
      421 tacttcgagc tcctctgcga gaactactga ccttgctgtc gcccatcgac tcatcggccc
      481 atcgacccat ccgcgggacc accggatccc cgggccggca gattcgagac cggtgccggg
      541 cagggtggga ggagggggcg gcggggcccg tccttcaatc cccgcccggc ctctccgcag
      601 gtgttttttt cggttcagag cttcccagac tatcaaaatc ccgcatcatc caagtttgtc
      661 cagttattat tccatcatcc tctgatccat gagccttcca ccaacaaatt agaaatagtt
      721 tgccggcgta aattccctgg gaggtatatc ggttgaaggt actggtatta tactacacca
      781 tgaacggcca cacccaccgg ctggccgagg cggtggccga gggagcccac caggtgaagg
      841 gagcaaaggt cgagatgcgc agggtccccg agaccctctc ggaggcggag ctggcgagga
      901 ggggggcctc cgccgaggcg gcggagatct tctcaggggt tctggtctgc agagtcgagg
      961 agctggcgga ggcggatgcc atagtcttcg ggacgccttc ctacctgggg aacatggcgg
     1021 ggcagatgag gagcttcctc gactccaccg gcgacctctg gaggaataac ctcctggtgg
     1081 ggaaggtggg aggggtcttc gtctcctccg ggtcccagca cggcggccag gaggcggcga
     1141 tcctcgcctt catcccgacc ctcctccacc tggggatgat cgtcgtcggc ctcccctaca
     1201 ccttcgagaa ccagaggagg atcgactccg tcgccggggg ttccccctac ggggcctcca
     1261 ccatcgtcgg tagagctggc gagaggtggc cgagcgagac ggagcttgct gccgcccggt
     1321 tccagggaaa gcacattgct gagatcacgt cgaagatcgt cggctgatgg tctcgggtcc
     1381 tgcccgtcga tcagctcaga ggcggggccc aatatttttt gtatatcttt ttgatgccaa
     1441 aaccttttcg atatcgtccg tttatcttgg ggcgcctccg catcgttacc tccgaggttt
     1501 tctcttgggc atcacctatt actccggttt aaccttgaat tagcctccca gataggggga
     1561 ttgaaccaaa agttatatat ataaaatgat accctataaa tacagcctct aaaaataccc
     1621 aagatccgtt ctttggtata aatgtggagt gaatggagat gactacaaga aaaattctct
     1681 taattctttg cattctgatt gcccctgtgg ccggagagaa ttcacctcaa tctgggggcg
     1741 gccagagcgc cccatatttg gacggtgacg cagatccgac cagtgaaaga tccatattct
     1801 ccagccagaa gattgacgat atttgtttct ttccgaatag ccccaagtcc agtccggata
     1861 gcactaaaat agcttatctc ctcggaaacg atgtcggata cctttattat ataatagcag
     1921 ggccggaggg gaagacgacc gagacggcct acgaatctat tcgtccagat tccttcgtct
     1981 tcagtccgga tggaaaacat cacggatata aagcttataa gggcggcaat tgggtggccg
     2041 taattgacgg ctcggaaggg aaagaatacg acgaaatttg gtggatattc ttcagccagg
     2101 atggtgaacg atacgcctac agagctaaga aggacggcca ggaaattgtg gttgtggacg
     2161 gaatagaaga aggaccgtac aaaggatcat ccgatcccat aataagctcc gccgggcagc
     2221 atgtgctcta cacgatggca gaggaatctc tcggccctta ccgtgataac gtggttgtga
     2281 acggggtggt ccaagagctt aaaggagttg atccggtagt cagccccgaa ggtagccggt
     2341 ggggttatta catcggttcg gtttatagag gacgtaccgg ctacatcgtt cttgacggag
     2401 aggccatcgc tttggaaaaa gagaagacca atcgcattga ccagatggtc tttagcccga
     2461 atggaagccg tttcgcttat aacctggtgc ctgacggtgg agcttacgga gaccacgtgg
     2521 tggtggtaga cggcattcca ggtaggagat atagcgagcc tggcgtagga aatatagtct
     2581 ttagccccga cgaaagcacg gtggcttact gggccaaagc tgacgacggt ggcttcatga
     2641 tggtggtgga cggtgaggag ggcaaggttt atgatgtcct taaaattggc gaccccgtca
     2701 tcagccagga tggaagtcat gttgcctacg ttgttgatag taacgatggg gcgttcgtcg
     2761 tcttggatgg agaagagggg aagaagtacc agagcatatg gggtctgacc ttcagccctg
     2821 atggccgttt ggcttacgta gcatctgacg caagagaaga agggtttgtc catctggtag
     2881 ttgtagatgg caaagaagat cagacatacc agagccagtt gtatggtcaa gggataaagt
     2941 ctggtccttg cttcagcccc gatggcgggc atatcgccta tatcgccaaa gatggagatg
     3001 gcgacgagtt cgtggtcgtg gactccaaag gatacataaa tccatggact tccctggagc
     3061 aaaaccttgt ttgggactcg gaggaggagt ttcactacat aggccaaaat aattgtgaag
     3121 ggcagacgcc aggagtctat ctagtaacag taacgatccc cctcagatga gatgaaagta
     3181 gccagactgg gaaggtccgt agaagtctga ttcgagagtg aatgatgcag ccggaaatta
     3241 tgaggaggag atctaaaata agtctgaaga aatggtatag aggatccgaa acgattagcg
     3301 gaggccggcg aaacgtatct cttttcacaa agatttctgc ttccgcctat agcttattga
     3361 tttagggccg gagccgggat tcgaacccgg nnnnnnnnnn nnnnnnnnnn tcgctcggag
     3421 gagataaaac tttccccgtt gctccgcccc cgccgagcct cccgggggcg ataggcctct
     3481 tcggcggtct cgaggacgag ggagaggagg tcgtcgaggc ggtttatgtg gtagacggct
     3541 cccagctctc cgagggcctc ctccaccagc ctgttcttgt tagcctcgtc gatggagaag
     3601 aggacgaacc ttcctatggc cgccgcctcc tggatggcct cccattcgcc atagaggttc
     3661 tgtatgtggg tgtcggtgat gcagagga
//
LOCUS       DASM01000005           23893 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_110490, whole genome shotgun sequence.
ACCESSION   DASM01000005 DASM01000000
VERSION     DASM01000005.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 23893)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 23893)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..23893
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_110490"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         4556 a         7060 c         7247 g         5028 t
ORIGIN      
        1 cccggcgtgg cggcggtggt cgtaggccat gcctttcctt cggcctccgc cggtgataaa
       61 gctgccccga cgatcgggca gaagggggaa agacgggatg gacaaggacg ggatgggggc
      121 gacggagaaa aatggggctg tgagccggga ggcgcggggg tgggcaaaag cgatataaga
      181 gatagcggcg agaggccgaa ggaggcacgg gggccggagc ctggggatca tatcggatca
      241 ggtccggccc gataattgaa tggagcttcc ggttttgaag ataatactga tgggaaaccc
      301 caacgtcgga aagagcgtcg tattctccag gctcacgggg atcgaggtga tctcctccaa
      361 ctacccagga accaccgtcg agtacaccct cggcaggacc tccatcgctg gggagagcgc
      421 cgaggtgata gacccccccg gcgtctactc cctggagccc tcctgccggg cggaggaggt
      481 gacgagggag atcttcgagg agggggcgga cgtcgtcgtc gacgtcgtag acgccaccaa
      541 cctcgagcgg aacctgaacc tcaccctcca gatcctggag cgggggctcc cgacggtcgt
      601 cgtcctcaac ctctgggacg tggcgacgag gacggggatt gagatcgacc tcgccgccct
      661 ggaggaggag ctgggggtcc ccgtggttcc ggcggtggcg gtcagcggcc aggggatcag
      721 ggagctggcg gaggcgatac cgagggctcg ggttcctacc ccggtgaggt tcgagtcggc
      781 ggaccagagg tgggcgcggg tcggcgagat cgtagagcgg tcccagaggg tcgtccacaa
      841 gcacccctcc ctcctcgaga agctggagga ggcgacgatc aagcccctct tcggcgtccc
      901 cttcggcctc ctcgttctat acatatcctt caccggggtg atcaaggtcg gggaggctct
      961 catcgacacc atcctcgacc ccgccttcgc cttctacggg gcctggatca ccgaggtcgt
     1021 cggccagaac acctccggca tcttgcgcga catcctcatc gggacatccc cggagatcga
     1081 cacctccttc ggcctcctca ccaccggcct ctacgtcccc ctggggatgg tcctcccctt
     1141 cgtcatcccc ttctacatcg tcctgggggt gctggaggac gtcggctacc tcccccggat
     1201 cagcgtcctc gtcgacgccc tgatgcaccg ggtcggcctc cacggcgctg cggtggtccc
     1261 gtgcatcctc ggcctaggct gcaacgtccc gggggtgatg gccacgaggg tcctggagtc
     1321 ggaaaagcag aggtacctcg ccgccaccct gatggcagtc tccgtcccct gcgccgccca
     1381 gaacgccatg atctgggggc tcctcggccc cttcggcctc cggtacatcg ccatcgtcta
     1441 cgggaccctc ttcctcgtct tcatctccgc cggcttcatc ctctcgagga tcgtcggggg
     1501 ggagtcgccg gagatcttcc ttgagatccc tccctaccgg gtccccgacg gcagagccct
     1561 cttcaagaag acctggatga ggtcgaagca cttcctgaag gaggcggtcc cctacgtcct
     1621 cctgggggtc ttcctgatga acgtcctctt catcgccggg gtcgtcgatc tcgtcggagg
     1681 cctggcggag ccgatcatca ccggcctctt cggcctcccg aaggacgccg ctgccaccat
     1741 gatcgtcggg tttctgagaa aggacgtggc cgtagggatg ctggcgccct tggggatgac
     1801 ggcgggccag ctggtcgtcg cctccgtcgt cctcgcgatg tacttcccgt gcgttgccac
     1861 cttcgccgtt ttcatgaagg agctggggct gaagaacacc gcgaggtcgg ctctgatcat
     1921 ggttttggcg gcgacgatcg tcgggacgat ccttaacgtc atcctgacgg tctgatttac
     1981 tttatattta agttaggctg tgtgaagttg aatttcactt catataaaaa aacattcacg
     2041 ttagggaccg ctttaagggc atataaaagg ttttcatcgt ttgagtggga tattatcagc
     2101 ccttccactt gttcgttcga ttcggaaaga ccagtccgga cccatcccat ataccgaaga
     2161 acttggccga gagtcgtatc ggaggactga tcacgtttta gctccaccac aagccaacgt
     2221 ggttcggtcc gatgcttagc caatagatct atccttccga ctgcacacgg gaattcgtaa
     2281 cctctatcag gatcgccttg ctcctcgtaa agcatccact cttttccgag gtccgtagcg
     2341 tcccaattat ctcgcaagaa ttcatgaaga tgcctctcta gataaaatcg ctgttcttta
     2401 gaggcagatg catccgattc ttcagagaaa tcaggcttac cgtcttcgta tcctttacca
     2461 tctgagtcgg atagaaactg ccaaagtaaa gagtccaatt cccaaagatt gatgttcaga
     2521 tcagacgcca gcgtgtttag gacctcgttg atctgctcat acttttcacc gaagcttgtg
     2581 ccacgtgcga aagacggcca gatatcaagc ttcttcaggg cctcttcaga cgttctattc
     2641 cagacgccgt atcgatccgg gtgggtgacg agcaaaattg ccgttactat tgcctttccc
     2701 atacctggga ccatatctac tgactggttg agcctataag aaattggttg cgcttcttcc
     2761 tgaagaattg acaatgcctt gcgcaactgg tccatgtctt tacagatttt tggccccatt
     2821 cgatgcaacc cggtccaatg acgattattc cggaaaatta aaaactcttt gaactccact
     2881 tcacttaagt tatacacatt ttttgatgcg aaaaccggtt gatatcgact gagaacttct
     2941 tcccgcggtt tcacgatttt agaatattcg gagtcatata ttttagcttt gttgagggtg
     3001 cttttcagct gtgaaatcgc ggttttatat gccccatcat ccatgttaat ttggtattct
     3061 actcattccc tttaaactgg tcggtcggtc taccttattt tctccagagg cacgctgccg
     3121 tcgtggaccg ccgtggcgac gagggcgccg gcgagcccca aatcctccag ccgctccaag
     3181 tcccgaacat ccctcgcccc gccgccgagg aggaggggat gatcgctgag atctgcggcc
     3241 gcacggagaa aacgctcatc gagccccttt gaggtcccca cccggtccag ggacaagagg
     3301 atgacctccc tgatggggag ggggttcagc ttccggatca ggtcgagggg cgggatcttg
     3361 agggcggggt cccgggatag gacctcgccc ccgaggagat cgatgctgac gactacgtcc
     3421 cttctcgaag acgcctcctc gaggagggat atggaccccg tctccgttcc caggaccggc
     3481 ttgaccgatg gaggaagaag gtccagctca tctttcgacg agatcccgat gtccgccata
     3541 gtctccgccc ttctggcgat ctcgccgatc aggtccaggt tctcgccggc ccccataatc
     3601 cgatcgaggt cggcgacgta gacctccttc ggccggagaa gatccaggat cgagaggggg
     3661 tcggaggttc cgacgatctt gctcatctcc tggatcggtc ggtacttctc ccgctccccc
     3721 cggacggcgt ggaccgccga accgttaaat aggtcgagga caaagacgca gcgcgtcatg
     3781 attttagaga acgaggatgg gacgatatga ggttgttggt cagtccgata aacgccgtcg
     3841 aggcggaggc ggcgatggca ggaggcgcag acatcctgga cgtcaagaac ccccgggagg
     3901 ggtccctggg ggctaacttc ccctgggtga tcaggtccgt cgcagaggtc gccgccggaa
     3961 gggtccccgt cagcgccacc atcggcgact tcgacttcaa gcccggaacc gccagcctcg
     4021 ccgccctggg agccgccttc tccggagccg actacatcaa ggtggggctc ctcaagatcc
     4081 gggaccggga tgaagcggcg gagatgctcc gacccctggt gaggtcggtc aaggagttcg
     4141 acgccaataa gaggctggtg gccgcggcct actccgacgc ctcccgggtc gggtcgatct
     4201 ctcccatgga gctgccggag gtcgccgccg agtgcggggc cgacgtggtg atggtcgaca
     4261 ccgcgatcaa ggacggcaag tcgaccttcg agttcatgac cgaatcggag atctccgact
     4321 tcatcgccct cggccacgac ctggggatgg aggtggcgat cgccgggacg attaagtttg
     4381 cagacctcgc gcttctaaag agggtcgacc ccgagatcat cggggtcaga ggagcggtct
     4441 gcggcgggga ccggaccggc gagataaagg aggagctggt cagacggatg aaggccgacc
     4501 tctcctgaag ctctttttat agctttatca tcttagcctt gatgaccccc gagacggagg
     4561 tgagcctccc cagggtctct tcggagacgg ggtggtcgac gttcaggacc atgatctgct
     4621 ccccgcccga ggccttcctc ccgacgtgca taccgccgat gttgatcgag tcctcgccca
     4681 aaacgacgca gcagggtccg atgatgttcg gccggtcctc gtggatcgcc atgatcatgt
     4741 aaccctcagt ggggacgtag acccggtact cgttgatctg gacgatcctc ccctcgttct
     4801 tgccgtagac ggtcccgtat accgatacgg agcccttggg cccccagagg gtgagggtga
     4861 cgatgttgct gtacccgttc gtcgaatcgg tcttgctctc agtgagggcg atcccgcatt
     4921 ccctcagctt gacgagggag tttacgaagt tcgcgctgtg gcccagggag tccagaaatc
     4981 ccttcaccgc cgatatggtg acgggctttg tatccttctc ggcgacggtc ccgccgtaga
     5041 cgatctccag ccggtcgtag ccgtcctccc cgagctgggc tgcgagccgc cccatcttct
     5101 ccaccaggtc catgaagggg cgcatggcag ccagggtctc cgaggagatg gcgggcatgt
     5161 tcagggcgtt ggggggtagc tcgcctcgcg ccaccgccag gatctgctcg gcgaccgcca
     5221 ccgccacgtt cacctgggcc tcggcggtgg aggctccgag gtggggggtg acgatgatcc
     5281 cggcctgatc gaggaggggg ttacctgctg gcggctcctt ggtgaagacg tcgacggcgg
     5341 cccccgccac cctcccctcg gcgaccgcct tcgccagggc ctcctcgttg atgatcccgc
     5401 cccgggcgca gttgatcagc cggaccccgg gcttcatgat cttgaactgg tcctcatcga
     5461 tgaggtttcg ggtctccttg gtgagggggg tgtggacggt gatgaagtcg ctctctctga
     5521 agatctcgtc gagggtcgca agcctgattc cgaggtcagc cgccctctcc gccgtcacaa
     5581 aggggtcgaa ggcgaggatc cgcatcccga aggccttcat cctcgaggcg atctcggccc
     5641 cgatcctccc gaggccgacg atccccaggg tcttgttgta gacctcgacc ccggtgtacc
     5701 tcttccggtt ccactctccg gccttgaggg aggtgttcgc cagggggatg ttcctcgcga
     5761 gagataacat catggcgatg gtgtgctccg ccgccgagat ggtgtttccg ccgggggtgt
     5821 tgacgacgat gatccccttc ttggtggcgg catcgatgtc tacgttgtcg acgccgaccc
     5881 cggcccggcc gatgaccttc aatttatcgg cggcggcgat cacctccgcc gtcaccttcg
     5941 tcccgctcct gatggcgagg gcgtcgtaat ctttgattct ctctaccagc tcttcggggg
     6001 agaggttggt gatgacgtcc acctccatct ccgtctccag ctttcgcacg ccctcttccg
     6061 agagagggtc gctgacaagg accttcatga caatctcctc catcggtgtc cagagatgta
     6121 caggcatttt accttttcga cggagtcgag acgggattgg ccgaggccgg cggaggttcg
     6181 attctgccgg ggcgtcttaa ggacctggaa ggaggaagaa ccgaacccgg attttccccc
     6241 gttctcagcg ctccgccttc ggcaggtctg tcacccgatc gaggggcagg ctcagatcgg
     6301 tgagccggag gagcggcatc gactcgtctg tcagatccga tagaagggtc aggccgaact
     6361 tcagggcccc tttctccgcc tcatgaggcg agttctcttc gaaccacctc cagatccctt
     6421 gagagagtat gctgatgagg gaggtggacc gatcttggcc gccggaggat ccccccaggg
     6481 agaccgctgg tccgtcgccg tcgaagggag aggaggggac gaagaggacc ggcatcgtca
     6541 ccctcggtag cccggggttc accaggtact catagatgca ctccagctgg agggtgaaga
     6601 gatccctcga accggccgtc tggttcttga agaggttctc gaagaacgaa gagagatgct
     6661 ctgccagggg acgacgaaga gcctctccgt ccggagatcc gacgagggag atatctatcg
     6721 gatcgtccca tcccagcttc ggacggtgcg ggtttgcgaa ctggatctcc gaggttccat
     6781 atacgaaggg atcggcggtc ttctttttgg ggacgagctc ctcgttacgg ctgaggctgg
     6841 cgacggcccg ggcgctctgc ctctccaaga cgttgagctg tttgatcatg accgtgcggc
     6901 ccggaatatc ccgccccctg taggcgggga gatactcccc cttcgatttg aacaggtacc
     6961 ggacctcgga gccgtcctcc aagccggaat cgaggacggt atcgtaggta tcggtatcgt
     7021 acccctcgat gaagaccttc ggtggggaga tcccagtgga gagccaatcc ccctttactc
     7081 tgatcatcag ggcctccacc gtggttccgt cggggtagtc aatactcttg gactcttcta
     7141 ttatgctgaa cttgcagccg tcggcaactt tgccccctcc atagcctccg gcaaattcgg
     7201 ctcccttcga cgacttccag gcgtcggcga cgccgccgac gagatcggaa aagaactcga
     7261 gggccttata cgccctctcc cgctctccct tctccgcctt tccgtcgatc ttccgcagga
     7321 cgccgtcgag gtccttcgcc acatcagggt agacggtcac aaattctgcc agggcggaga
     7381 agaggtcgtc gatgggtgag gatagagctc tcaacccggg ctggaggttg aactccaccc
     7441 ggaaggagat ctcgtcctgg ggctcgtggc ggctctggct gtagtcgaag gagtaggccc
     7501 agatcaccgc ctcgtcgatc tgggaggggg tgatctcttc gagctgcgaa agccccgcct
     7561 ggccgatcat cgatggagcc gtcgggatgg ctcggaggac gagggggacg tcgaaggagc
     7621 cgaggggctt cgtcagggag aagtctcctg ccggcggcgt cacgaaggag agccaggacg
     7681 acgcctcgta gccatcatct atccccggaa gccttccgat ctcgtgctcg atcatcgatc
     7741 cctggaacga gaggtccagg ggtacgtagc ttttcatcgg ggtcgatgga tcgtcctctt
     7801 ccttcgtcgc cttcgtctgg aggaggaacg tcagcttctt tgacccctca tcaaggggga
     7861 tcttcgccgc cgtcagggat acatccccgt tctgagactc tccgccatct tttggagccg
     7921 ggacgccgaa gagacggggc gggctggagt agcccggatg ggggatatcg gcgtcgacct
     7981 ccacatcgaa ctggatcacg gcgtcggtgg cgtaggcgtt ggagagctgg accagaagct
     8041 gctgtctata cgcctctttc gcctctgcca ggccgtcatc ggagtcgcca aaatagaccg
     8101 gagacgcgag atcgcttatg gagctggcca gcgatttttt atagcccaaa agcttctcga
     8161 tgaagccctc ttcatcatcc ttcatcagca gacgggccgg agagagatac tccggcgtca
     8221 ggaacctgtc gaacgccgcc aggaactccc gggcccagac gtccatgtcg actccgacga
     8281 agtcgagatg gatctcgtca tcggaagcat actttttgat gggcactttc gatcgcgact
     8341 ctggcctgtt ggagatcggc cttggtgcga atatcttcgg agccccggga taatatttca
     8401 cggcataaga tatcccttcg ccaccttttg cgcccaagcg caccgcccag atagacttcg
     8461 aggtcgagga ggttccgatc cgcctcctgt cggtgccggt ggcgaccttc agctggaacg
     8521 atccggggag gctgagggcc tcttcgaacc gggtggcgaa gctcttcaga gaatagggat
     8581 cgccggaaag gccaatatcg atcgccggcg ggatgttggt cgaggaagag cagacccctc
     8641 ccgcctcttt cagctcggcc tggatcgccg agacgggccg ctccatcctc atcgccaccg
     8701 tcagctcgaa gatctgggca gagttcaaag cgtatccctc ctcagccgcc cccagggcga
     8761 gggcgagccg gatctcgtgg tccaaaggcc taatggccga ggccgctctg ccctcggcga
     8821 cgtcggagag gaactggtag atatcttcga tccagccccg gaggccctgg atttgatcct
     8881 tcgccagctc gagggatgcc tcctccagga ggctcgcctc tatggagatc ttaatcgtct
     8941 cctcatcgga tccggtggta ttcaagagct gatagtaaag ctgcctgtag actcgcaggt
     9001 cttgggacgc cttatctttc ttcggatctt ccccgacgat cccactctcg tctgaagaaa
     9061 ggtaatattc cgggtcgaag gcgagggtga tggtcagcct cgtccccgca ccatccttga
     9121 ggacctggta agagctggag acgctcggcc acctgccgag gccgatcagc tcgtcggtgt
     9181 accccaggag aatcggcggg ttgtttgccg gcgcggtctt gtccagggac ggatcggata
     9241 gcggcgttct ggcgacgttc ccgaagatgt cctgccagcc gaagttcacc tggaggaggc
     9301 ccccgatccc ggcgtagggg ctctcgctgg ggtcggggat gccggcgtcc ccttcggagc
     9361 tgccgtgttt cgcgaagcgg gggtagggga gggcctggtg gtactcccag agctcgcctg
     9421 gggccttggg aggcgcagcc gcccggatct tcgactcccg tcggtccgcc gtctccaccg
     9481 gccccatcgg aagccccctc tcgctcccgc agaagtcgag gttctgaagg acccggtatc
     9541 ccagcatcga gaaggtctgc tggaggtaga gctggccgta atcgtcccca tcccccggga
     9601 gatcggggac ctcggaggcg acgacccgtc tcgccccgat ggccacggcc ccggcgggga
     9661 cggtggcggt ccggaccttg gggccgcctc cgatcttcat cggagctgcg ctcaaaaggc
     9721 ccgcgacaga cctgttatcc tcggccaggt cggcggcgga gcagccgtag aaggcggcga
     9781 ggtcggcgaa gctcttcccg caaccgtcgg ttctgattgg tgccgtgatc ttcggaaggc
     9841 ggatggcatc atacgggacc aggagatgaa gtggtgctgc cgggctcaaa agccccttcg
     9901 tcgagcaatt cacttcggcg aggtcagcga tggtggtgcc gtagaaggat gcaaggtcgg
     9961 cgaaggtctc agtccccagg ccgatcaaga cttcgatctc cgggatccgg atcaccaaac
    10021 ccggctcgag gacgtagggc cagggatcga tctcggggtt cgcctttatg atctcctccc
    10081 ctggagtatt gaagtaggac gcgatatcct caagtctgcc tccgggggga agccccgcag
    10141 gcctcacctc gtaggtcctc tcggagattt tgaccggctt accctccgtc agggggtggt
    10201 cggtgtttgc ctcggccagc tgagacggag tgatctggta acgatcggcg atggtggcca
    10261 gggaatcctc ggggccgggt ttgcattcaa aggcccttcg ggggttcgct tctttgatcg
    10321 cctcctccgt ggtattgaaa tatgatgcga tatccgccag cctgcctcct gggctggtcc
    10381 cgatggggct gaccatgtaa gttccagcgg agacgaccac cgacctccca tcagccaggg
    10441 gatgagacgg gtgatcctcc accagctcga tgggggttgt ataatacctc cgggccatct
    10501 cgtccaggga ctcggtcgcc ttcgggatgt ggtcgaggtc gaagggttcc gccctcccat
    10561 agaccacgga cctcgacgga tcgatcgggt ctccggtgac ggcacagttc acgtagcttc
    10621 cgaccccctc caccccgggg acccggggct ttccatagat gaagaggaga gatatggtcg
    10681 catcgccctt ctcgttgaat atgctctccg ggaggcccga gtcgtcgccg gagctgtagt
    10741 agaggtagta tcctccggag cgggtgatgc tgttctccca gagcatcctg acgaagtcgt
    10801 aggcggtgtc caggcaaccc gccgtcctgt gccgcttcgg ggccgcctcg gccagagctc
    10861 cccggcccca tcggaggaga tccgacggcc tcgtctccgt cgtcaggttc acctggctga
    10921 tgaagatggt gaggtcttcg tccccatccg actgaagata gggatcgacg ctgccggggg
    10981 ggctcggacg atagaggagc cggatatctc cgatcgcctc atccgagccc tggatctccc
    11041 ggaggagccg ctccaggagg aggatccccc gctcgttcgc ccccgtcagc tcgtaggtcg
    11101 tccgggtgga ggggctctct cccaccggct cgatcctctt gatccccacc tcgataaggg
    11161 tcccccagcc gtaggacctg acggggacct gatccatatt cgaggtcgcc tcgttgtagg
    11221 tcccgacctg gacggagatc aagggcttca actctccgcc gcccgactcg gccagctcca
    11281 ccaggctcgt cggcaacgac cagatccgga gggactgccc atcgccggag ccgtcgtcgt
    11341 gggggaggga gaccctcccg ggatggttcc agaccgtcgc cgaggggaag gggtaggtca
    11401 tcggctggga tcgggagacc ggctccggac ccagggcctt catcctgggg acgatcccat
    11461 ctcggacggc cttctggact tccaccaccc gatcccgatc gtcccccgta atgacgaccg
    11521 atagctccgt cttgctcgca ccgcccacga agtctaccca ttcgaggccg tcgtcttttg
    11581 ccagggagaa ggagaagaag tggtcctcgt tgagggcggg gatcgggaac tgctggccgg
    11641 tgagggcgta gaggccggcc tcctccggcc aaacagaggc ctcgtcgcag aactcgatcc
    11701 cctccgtcgg gagcctcatc ccgtggagga ggtatcggga ggcgatcccc gagaggtggg
    11761 atagccccat cgtcctcagg atctcagcga tcagctcgga gacccggaac tgccggaggt
    11821 gggggacgtt caggcaccgg gcctcaatcc catcggaggt cgagaagagg tcaggaacct
    11881 tcaggttggc ctcggcctcc gccagccgcc ggaccgggat cccatactcg gcagccaccc
    11941 cctccaggct ctccccttct tcggtggtgt aggatgccgg tggaagggcc agaagggcag
    12001 aggcagccag gaccccgggc ttggttgcca ggtccgatct atcgagaagg tccgccagcg
    12061 aaccggcgaa gagatattcg gagatcgact tcaaggtgtc ctcatcccgg ataacataat
    12121 ccggctttcc ttcgcaggcg agggcgatcc cgaccgtcag gatcttctcg gaaaggttag
    12181 cttcggcaag ctttatgact tcaacctctc ccctctcggc gatggaggat agggtatcgg
    12241 aagcccccac ccggtgggtc cggccctgga tctcgaggtc cttttggggg ttcaggaggt
    12301 gctggccgtt cgcctcgaag agatccgcgg cggtgaaggg cggcgggaga tcgccgatcg
    12361 gatcgtcccc cttgggagcc atatgctcgt ttatccagcc gacgatatcg tcgatcgtct
    12421 catcggatgc ttttccttcc ttagaggttc cgatcaggta ctggaagttc cggaggccgt
    12481 ctctcgcgtt ctggaccatc tggcgggcca ggaggaggaa gtaatctgcg aagacgaagg
    12541 aagcgaccga tcggccggga ggttcgggtt cgtcccccaa aagaaggcgc cggtccaagc
    12601 cccctcccat ctcctcttcc acctggaccg cgagctcgtc gaaccgcgtc ctcaggtcgg
    12661 caagatattt ttcgtccacg gcgttgaact cggagaactt ccattccagg accttagaat
    12721 cgtggtagga ggggaacctc agcctcaggg ccggaggcat cgggaatacc gcagcgtccg
    12781 cccctccggc ccccccatcg tcggcctgtg tcctgtatgc cacctgcaga gtgaaatggt
    12841 cccccatgaa gttggcggca tcggcgtctt cgataggcat cgagaccacc ggatccgaga
    12901 ggcatttcat gatcgctaaa agctcgtcgt cggatacggt gtagccatcg atctcgtctc
    12961 tggtcatcgg ccggtccagg gccgcggcga ccgcccaccg gaggacgtgg ttgcagagcc
    13021 gctcgaagga tgtgatcccc gtcgtcccct cttccagccc aggaccgtcg gatgccgacc
    13081 tcgtctccag gaggagcatc gggacgtagc aggagtgggg acctgcggcg ccatggatct
    13141 cgtcggtcga ggcggagagg accggggcga agtggcagat gaggatctct ttctcttcgg
    13201 ccggcaggta gttatcccag ttcaaaacaa gcccgcctcc cccggaggag gcgatggccg
    13261 ttggggtcct cagcctgagg tcttgcctcg accggagggc cgcatcggag ccgtcggcca
    13321 gggtccaagg cggggtgccg gagtttttga tggtgaaggt ctccttgatc cgggcggaga
    13381 agctgaagtg tatcgtgatc ttgaagaggc cgcagttgat cttgatcgat agggagacgt
    13441 cgacggaggc gatgacggag atgggtatgt ccttatagca ttcgtaagtt atctgggcta
    13501 cgagggagag ggtgacgttc acctcggcct tgacgatccc gaagtcgacg ctcccgaaga
    13561 gcctgcccat gatccccagg gtcccctgga gccagtagta gtagtcgacc tcctttccgg
    13621 catcgtaggg gttccaccgg gcgatgaccc cctcgaggat ccccaccagg gtcaggctga
    13681 tcccggcccg gaggaccccc ttatcgaggt actttccgac ccccagctgc agccccaggc
    13741 cgaagacgat gacggggttg aagttcccgt cggtgacggc ggggacccgg ctggtggtgg
    13801 caccgctgag ctttccgaag tagatcccgc cggagccgat gacggggacg aaggagatct
    13861 gggcctggat ggtgaaggac cgggagaagt tgaggttcca ggggaagccg acgtccacct
    13921 tgaagtcgcc gttggtgtag agctcgatgg cgaagaccgg gagggtgatg ttgaaggccc
    13981 ccatctggaa attgcgcatc ttgtcgggga gctggatctg ggcctggtag acccccaccg
    14041 tctcgctgat ccggcggtac attatctcga agtcgaggcc ggccaggacc ttcgccgccg
    14101 ttcccgcgag agcgacccgg aggccgtaga tgttggggtc gttgaagacc gtctggaggg
    14161 agaggaagta ttcggggggg gtctctttga acttcagaat cccgaagtcg gcggccacca
    14221 tccaggagct ttcggggctg aaggcgacgg cgggaagctg ggtgggagag gggacaggga
    14281 gatttttgat cttcttgatc gcatcttcca ccgacttgca gtccgtgatc ccctcgacct
    14341 tcacgtgctg gccgagggcg aggaggcgga ggtcgaagta cttgttcccg ccgccggggg
    14401 cctcgggcgg cttctggttg aggaggtccc agcttagatc gtcctttttg cccaggaagc
    14461 tgccgacgat cttgatgtat acggtatttt cggcgccctt cttgtcgtag gtgaggccga
    14521 tggaggtgat ctttatgaag ttcaggtctg cgtttatcag acagtcgaag ccgacggtat
    14581 tcttggtgag gttgatcctc aagaccaggt tcttgaggtc gatctggtgg aggacgttcc
    14641 aggggggttc gagctcgaag tcgcggctcg ggtccaccag gtggacgagg aagtccagga
    14701 tctcgccgaa ggtgaccccc cccaggttga cggtgaggac ggtatcgtta tctttgttct
    14761 tgctgacggc gcagacgagg tggaagccct tgtattcgat ctggaaggtg acgtcctttt
    14821 cggcggcgtc gaagccgtag acgatcgaga ccgtcaggga gttgacgctg atcctacccc
    14881 tgacaaagcc cgagtaggtg gggtccgcgg tgggagtcgt tctatgcgac tggagggaga
    14941 gctcggcctc tagatcccat gttttaaaac cttccagggt atacgtccag cccgccacgg
    15001 tgtcgaaggc gaactcccga gtcttggtgt caaaggatgc cgatagcccc cgcagctcga
    15061 tcgctccgac ggatttgggc agatccggcc attggttggg gacgaggttc ttcaggaaat
    15121 ctttgagcgt tacgcctccg ccggtcggga ggagatagcc cgcaaaatgc cacccctggc
    15181 cgggggcggg gtgagatgcc tcgatggcga aggggagctt tgcgatctcg acctgggcca
    15241 cgaccgagcc ggagaggctc ttgggcgaat aggagagttc gaagccggtg ttcttgaaag
    15301 cgaccacggg ctcgaggggg ctccagcccc cttcaagctg cgtataaaag aagagcgtcc
    15361 tcgatcccgt atctgctctg aggctgaggg cgctcacttc caaatcgagg tcgatgtcga
    15421 ttccggtgaa gtatttgaat actcgatcta tcgatatcgg gtaacgtgta tcaagatttg
    15481 cgctgatgac gacggagggg gcgacgggcc cggtcgatcc ggagaggtcg acttcgacgt
    15541 tgaggcgtgg cgcatccggc tcggagccga agaggaccgt tccgccgacg ttgccgagga
    15601 gcaccggact tgagaaggga ctcatgatct gccactccgt acagacgtcg gtgatggaga
    15661 tgccgggaat tggggtcgac caagtctctg gggagcggat gctgacgccg aggaagtcta
    15721 tgaggggccc gtctcccttc ggctccaggc cgacggtgac cgagtcgaga tagaacgacc
    15781 caaatttatc catgcccgat gggagggtga gatcgtgtcc tcccacgaac tccgtcagcg
    15841 ccgagaggcc gtttttcagc gaatatctct tcgggtcctg gaggccgatc cgcagcaccc
    15901 agacgaagtt gccctgcagg agcggcccct ctatgaccag ggttggcaga tccggtatcg
    15961 ccaccgatcc gttgatggtg aggatcgaag cagccgggtc gtcgccgctg gagtcgatgg
    16021 tccgcaaccg cagataaatc gaacgcaatg gatcgatatt aatgtcagtg acagaagcgg
    16081 agaggtccag gagggggaat gtgccctcac ctctcatgac gatcccgcca ataagcttga
    16141 gagaggtggc ggcgggcatg tagcttttga ggtgctttcc aatggttccc gccgtcacgt
    16201 cgagggaccc ttccaggctc agcccttcag gatgcttccc ctcggggtcg ggataggtgt
    16261 agatccaaaa ggtcggcgaa gatatgccga tgtcgttata gaaagacagt tgccacgaga
    16321 ggccaggatt taagtaatcg tagcccaggt aatccggaaa tgaagcgaaa ttatcagcga
    16381 acttccaggc cgaacctgag agcgggcttc cagacaagtt taaaagaacg ttatccgccg
    16441 atgggacttg gccggtgatg gtgacattat atatgatatc tttaaataaa gccgccttcc
    16501 cgacgatccg gaccacgccg ttggttttgt ccggctggac gaggttctgg ttgggaaact
    16561 gatcgatcct caacgatgaa ttgtcgtgct tcaagtaacc gacgagcact cccgcctgct
    16621 taaagaggtc cgggcccaga agtaagtact gcgatccgct cttgccctgg actgcacctt
    16681 gcaggcatga gatcagatca tccagattct tgatcacttc gctcaatatt tctcacatcc
    16741 gatttatcca gagtctcgtc ttcttttagg gattgcagaa agatggtgtt agatttggat
    16801 cgggcacagg ccataatccg tctgtcatat ggtcgatctc agcgggcttg ccttgatcga
    16861 tcttaagtgt ccactggacg ccggtataga gccctttctt tccactgaat tgctttccat
    16921 cataaccgat tctattctca tcagcgccgg ttacaacata ccagaggttg ttgcagattc
    16981 ccaagttggt gctgatgttg cgatagtcat tatctttacc tgggcaggta cagatgaacc
    17041 agtgtgttcc acctatttta tcaagtgtgc ctttattcat cactctcatg atgggtggac
    17101 aatatggatg accccatact ttgtcagctg aaccgaagat ggctttcggt ttgaccacat
    17161 ctatccattc atcgcaagtt gagccatcac ttccatggtg tcctaacttg agaccaatgg
    17221 attgaagaaa attcggatct tgtttttgat attcatcgac gatatatttt tctacagttg
    17281 agtctgcatc gcctggaaga attaccttgc gaccatcgta ctcaaacatc agtacaagac
    17341 ttttgttgtt ggttttgttt tttgttgaag gaaaattcga actcaggagg tagatattga
    17401 ccccattaat ggaccacctt ggattaccta tcatgtcgta ctgtttgtct gcaagattta
    17461 cattagattt gtttttcaaa acactttcga tcttttcaaa tgtcgcttta tcctttgtct
    17521 tatattcagt ttcccatttc ccaccaacag ttaagttatt tatacatagt tttggattaa
    17581 aactatcaat aagatcgccc aacttgttcc aatggtctgc atcactgtga gagataaaga
    17641 ggtgatctat ttcaggataa gatggtggtg accgactctg acaaattttg gtaatccttt
    17701 cattaaggaa ttttaatgct ttcgtatacg gtatgttgga tggtgttaaa ttatgactgc
    17761 cgcaatctac gagcacgatc ggacccttac cataaggcgg catctcgaca agcgtgcagt
    17821 cacccatgcc tatgtcgaga aaattaaaga cgatactatt tgacattgtt tacactccat
    17881 ggtttttgcg aatagagttt cgtatcaacc gttccaaaag actggagggt atcgaacccg
    17941 gttctttgct gatcctacgg ccgcaaatga cgatccattc aggccgaagc atcaactcta
    18001 atccctttcg atctctgaag gccacagacg tcgtatcgaa caagcccaag aaggccgccc
    18061 ccacttcgcc actcatgcct ctcccttcac atcttttttg tacatcgcat accacccgag
    18121 gccgcacccc cgggccccgg ggtcggagtt tccaaagagg tagaagctgg tggctccgcc
    18181 cggcggaagc ttcgctatcc ccaggagctt gagggcgatc tcggtgaggg tgaggaggaa
    18241 ggactccctc cccccctcct ggtcccggac gtagtagagc ctgacgtccc cgatggagag
    18301 cttgaggacc ccctggaggt cgaggagctt tgcgccgccg ccggtcccgg gaagcttgat
    18361 cccgatgaag acgttgtagc cctcggagcc gccgccgctc caggcgagga ggaggctgga
    18421 ggtgaggctc accttccccg ccagctctcc gggggtcccc atgttcagcc ggaaggcgag
    18481 cccctgccag gggccggaga ggccggcctg ccggagggag gtcttcacct ggaggaagcc
    18541 gccgtcggag ggcttggtat cggccccccc gatcacaaag ccctcgaagg cgaggccgaa
    18601 ctccaggaat agcgacttat cccggggggt gctcttctcc aggctgaagg cgatcttggt
    18661 ggggtcgaag cgaaactcct tcttctggct ctcggaggca ggataggtca tatccagccc
    18721 cagccccgag aagctgagcc ctcgccgggg ctcgtccttt ccgtcctcgc taacgctacc
    18781 gaaggagaat acgtcgaagc tgacgggccc ctcgctcgac cactcttcac cagccccatc
    18841 cgtctcctcc ccctttccca cctgcaggac gtggaagtcg aggaagcccg tcagcccaaa
    18901 ccgcgaggag acgatgccat tgttctttcc gagggtgctg aaatcggcct ttgtgatctc
    18961 caccttccgc aggacgctgc tatcgaggta gaaggagttg tcaccgacgg agtcgaggac
    19021 gtagaccgtc tcgccgccgt gctgctgaca ggatccgttg aagaggatgg cgttgtagag
    19081 ctttttcggg tcccccgtgg aggtgacggg atggccgaag atcctgttga tcgtcagctg
    19141 ggcgaggctc tcgaagtcct tgatcgccga ggactcgaag aggaccttca tcgtcaggac
    19201 ccggaagtcg taatccgccc ccggcgccgg cggcacgggc cccgacccgt cgtagcctgg
    19261 gtcggagtag tagatcagcc cgaagaggga gctggtcgtt atgtcgaccg ccctcccgtc
    19321 aaccttgctc ccgtcgatcc cgaggtggtg gacgttgaag ccttccggat cgtcaatggc
    19381 gccgatgagc gcctccagct gaggcggaaa cttgtcgcgg tcgatgtcga ccttcagggc
    19441 gaggaccccg ttccagctcc catcatttac tatttcattg aaatgcttaa agtaatcccc
    19501 cttctcgccc gccgcatcgg cgatgtactc ctggagccac tgggagacgg cctccggccc
    19561 gccagatcgt gggtcgctga actcgtcggc atcggtccat agcttggagt tcttcgccct
    19621 ctccgccagg gtcccctcac agaacttgaa gatcaggacg ttccggtaat cgttgaagct
    19681 gttgcccttc ccgacgttga tctcgaaggg ccagcccccg atggagatcc tgttcttgaa
    19741 ggccgtcttg tccgaccccg atacggcggc gggttcggag accaccagga agagctggtt
    19801 ggtggagaag gcggcctgca gggcggggtc gatcccttcg aactccagag cctcctcccc
    19861 attcctggcg atgaggagag aatcccacct cccatccgcc gagacccggg atatgaagcc
    19921 ctggggggtg gcggcagggg tggtctcgcc caacgatagg gatctagcgc cgcctttgac
    19981 cccggagagg atccggcccc tccggaaggg gctgaggatc tggagctcga agttggggat
    20041 ctcgtcctct tcgaagccgt ccccatcggc ggccactaac ccgtacggca ccatcgggta
    20101 ggccaactcc ctcccgaggc gggccgaggg cctctggagg tggtcgagga tccccgaggg
    20161 atcggcagcc tgacctccgg catagagggg cgccccctcc ggctgggaga agtagaagtt
    20221 ctcctctccc cccgcagggc ggttgacccg gatccaggag gtggtgaaag tcccgtttaa
    20281 gaggggctgg gcccccgcgg ggtccggatc gtcgagggat gcatctttta tgggaaaggc
    20341 aggggcaaag gcgggaccat ccggaacgaa ggtcaagatg tcgtcggcgt gaaagccgat
    20401 cgtctcaagc cccgataggc cgcagaggag gcgggggagg gggcggcctt ccgcgggggc
    20461 gaacttctcc ggatcctcca ggccgacggc aaaatccccg gagggcgcga gatagaagga
    20521 gtcctcctgg ggttttcctg gtatttttga tctggtttta tcggcggaga agacgagccc
    20581 cgatctctcc gcctccggcg tcagggcgac tccgtggccg agggagaggc gaaacccgct
    20641 gggaaaggtg gggggggttc cggcgaaggt gaacttcgac ctctggcggt cgagggggtg
    20701 gatcggatcg aggctgacgg tgaagggtac catcgtccct ttggcgaggg gggcgaagac
    20761 ctcgtacggc tgggaacgga tatctcccga atcgcctttg cagaagtact tgaggcagac
    20821 ccgggcggcc ttgaagtcgt tcttttctgc gtcggccctc agagtggcgt cgaacctgat
    20881 ggcgcccgga gcggctcccg tcagaggtat gcgaatgagc ggggacctct cttttggcgc
    20941 cacctctccg aaataactgg acccgatctc attatatagt ctgatgtttt tgccggcatc
    21001 cttcgtcaac tggaattctg cgttctcctc gagggttatt tctactcctt tatctaacct
    21061 aaattgtata tttcttagtt tgatccagtc ttgtctgtta attccgacta cctgtccctt
    21121 atcgctggat agctttttaa tcggtatata ctgcccaatc agacctgcag agtttggatc
    21181 agaaaaccag aatatatacc gatgatagtc tggataaacc ccattgattt tcttcaagaa
    21241 atccttcaat ttcgcgagga aatccccagt tttctccgga tcccggccgc agaataagta
    21301 agatcctccg ttcatcctct tcctcttatc accccagaaa ttcgtccatg atgccccaag
    21361 gccgacaatc gggccaccgt cctgggatag aagatagcct atccacacgc ccccttccgc
    21421 gccgtggcca tagagatggg atccatcgat actgatcaat tttttgaaga aatcttggtc
    21481 gctcataaga ttacgccctc tccttacggc ccgatggctg gacggccccg atcgcagcga
    21541 ccagcccctt ccagggttct gggaccggcc ccaaaacggc cctgccagat tctacaccaa
    21601 gagctggaga ggaccccggt cccggcccaa aggccctccg atgttggcac gagatcgcga
    21661 gatcctccgg gagaggctga aaaggatcgt ccatctcaga tctcctccgt atcaatctcc
    21721 atccagacat aatcgtcgtc ggtctgcgac tccatccgca gacggtagtt gccgtctgtg
    21781 tcaacggtcg cagtacgatc gtttgcctca gcttctccat gaattaaccc tcgcaggacc
    21841 ccctgaagct tcactccggt ggaggctccc atcgaggcgg tcatcctccc cgaaaactcc
    21901 gcgtcggtgc cgcccgatgg ttggatatcg accgacgctc tgctgtgctc ttcgtcgcgg
    21961 atgaatcctg ggacgccgac atgaaactcc ttcgactcgc tttgaagatt gatcacgccc
    22021 gccgcggcga tcagatcctg ccaggaggca gaaccatcgc catcggttct gacggaggat
    22081 tcggatctca tggatccgtc ggagacgtgg ctggagatct cggtgtagtc gtttccgggg
    22141 agccagtcga ctccaggata gtggcgaaat ccgctcgttt ttatccccac gtcgctgccc
    22201 cgaccctccg acctctgata tgccgtcgca gccccggcat cggcgtatcc catcccggag
    22261 aagaggatat cgccttcgaa gatccagctt tggatggcgg ccgatgatct gctcgcgggg
    22321 ctgtgggagg tgatatccag gcgggtttca tcccccgagg aagccgcatc cagggtcgca
    22381 gaggcgctat ttccagtacc ggtggtcatg atggttttgg tcctgccatc atacccctcc
    22441 accgaggtct cggcccagga cccgtcccgg tcggtcgccc ccaccgccgc gaggccgtag
    22501 ctcccctcga aggccgccgc ctgagatgcc ccgaagccct cggccttcag gctcgcaccg
    22561 ccgaccatcc ggatcagggc gtcctcggcc gagaatcggc tcatcccgga gtatccgccg
    22621 cttccggcgt acctctggag ggcatccgcc gaccccgccc cctccatcca acgggagtcg
    22681 atgatctcga ggcggtcgaa gctcgctctt gtaccgcccc tcacctcgac gccgtcgctg
    22741 acctggtagg agtcgatgat ggcgacggag ctcccgggac cgctgctggc tgaaaaatac
    22801 atatctatcg ccagggcgct aggtgagagc ataatatata ttaataatat aatgatactt
    22861 tgcctcatcg atcattctcc catatagact gaagtttctt ggattttatc attaataaat
    22921 gttcctggtg aggacggagg attgaagaaa gatacgccca tccgtcgatg gcgccggaga
    22981 ggagatggca aagggcttgg ggatatttat ggactgctgc catttaggat cttctccttc
    23041 cggcggatct ctcggaggga gaaaaaatga gaataaatat atatcttata aatcaagcta
    23101 caaatgtata tttaatatta tagataaaga ttagaatcta taacaactga atatatttat
    23161 cattgtctct tttcatatgg tacccgcacg ccctaaccgg agcttgaagg ccccgccccc
    23221 cccccccnnc ctcggccccg gattctatct tcgcctctct ttcccccccg gcccctccag
    23281 aaccacctcc acctcgtccc catccgatag gccgagggca tcccggaggt tgacgggcgc
    23341 gacgagctcc agaagacttg gagaatagct cgtccgctcg ggcctcacga tggcacccct
    23401 tatcccgccg accctgacgg ggcggcagat gcaggcgccg aaggctctcc cctcgtcaat
    23461 gaagccctcg atcctgatcg aatcggggtc ggagtcgtcg aggacgaagg gctcttcgag
    23521 cttcaggttc agggtcccgg ggatcgggtc gaggccgagc ctctcggaga actgcctccg
    23581 gtaaccctcc cgagagatgt agtaccgacc cacccccagg ccggaggcga ccttcccccg
    23641 gagcctcatc ccctggccct cgtcattgac cgaagaaaac ccctcctagt gcttgaaacg
    23701 aggagaggat cgtctaggga tttatataac ggttctcgcc gccgtgggga cgccgggatc
    23761 gtcgatcaga cgcctgcgac aaggcctcag aagaatctgt ccgaaaaaag ggttgggcgg
    23821 gtcccgcccg ttgtccctca ggcgtactgc ccgaaggcca cctctgagat ctccttcgtc
    23881 gtctcggagt gag
//
LOCUS       DASM01000006            4499 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_88914, whole genome shotgun sequence.
ACCESSION   DASM01000006 DASM01000000
VERSION     DASM01000006.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_88914"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          877 a         1373 c         1329 g          920 t
ORIGIN      
        1 actccgtggt ggcggtgacc accgggatca tgcggcagct caccccggcg gagctggagg
       61 cggtcctggc ccacgagctg acccacgtca agaaccggga cgtgatggtg atgacgatcg
      121 caagcttcct ctccacagtg gccttcttca tcgtcaggta ctccctctac ttcggcggcg
      181 gcggctccag caggaaaaag ggggggatct gggtcgcctt cgtcgcctcc gccgtcgtct
      241 ggatcataag cagcctcctg ataaggtccc tctcccggta ccgggagttc gccgccgacc
      301 ggggtgccgc cgtcatcacc ggccagccct cgaacctcgc ctcggcgctg atgaagatca
      361 gcggggtgat gccccggata ccgaaggaag acctccggag ggcggagggt atgaacgcct
      421 tcttcatcat cccggcgata tcgggatcct cgatcatgaa cctcctctcg acccacccac
      481 cggtggagaa gaggatagca gccctccagg ggatcgagcg agagatgggg gcggcatgag
      541 ccggacgggg gccgaggcgg agaggatctc tatcgagggt tctgatcgag ccggcacgag
      601 ggggatggag aaggcatgag gctcaagatc ctcgacgcaa tcctcggaaa gagcacgctg
      661 ccgaagtcga agctcgatcg gctcttcgcc atggccaccg ccaggatcac cctggaggcg
      721 aacctcggcc tatccccgtc ggggtctgcg gggatctgcc tcaagccgat cgactcctca
      781 aggtacgagg cggcccggtc cgagatcgag gctctcctcc attacagcgg ccaggagacg
      841 gggacgacct ccaggatcga gatggacgag taccggtacc tctgggtcgt cctggaggac
      901 cgggacttcg acgacctggt ggcgaccgtc cacctggtga gcacgacgat gaccgaccac
      961 ggcctcggcg agcagctcct atgcgccctc tacagcttta ttgggaaaaa cggtcccgtc
     1021 tactggatat acagcttcaa gggcggggcc tactatccct tcgcccccaa aaaggggcat
     1081 gatagggaca acgccctgga gttccggctc aggtcggtga tggagccgga gctgccgata
     1141 gagaaggacc tggagaagtg gtatcccctc tgggggatac ccatatagag tggtacaact
     1201 ttaagagata aacagatttg tcgctccacc taaggtcaaa ttttggaggc ttcaaggagt
     1261 aatagttaaa aattgtaggg caagtttcct gtgaataaga accggtcatt tgcagaattt
     1321 acatccgatg tagtcgctta ccagagatgc tctaaccgat gccattgtaa tatccatcat
     1381 ctcgaccaga tccgcgtgct ggatcggcca ttctgacgga tcccctggtt gacgtcgtta
     1441 tggtcttcca cgactaccgc agcgttggcg gcgctctggg ttatgtggtc tccctgggga
     1501 gccagctaat tttagaaggg agtggccagg agagagcaat gaagggggat atttgttgtg
     1561 acctctcccg accgcaggac agatcatcgg tgatgaatgg tttactgccc tacggacaat
     1621 agactgtacg acctcccaga aaagccagaa cctcgcagcg cgaaagtaat ccgagaggcg
     1681 cctccagccg aaccctcctt ggatggactt gaagacgaag aaggcttcat ccaaaccatt
     1741 gcgatgagag ccccgcatag ttcgagatcg aaacgagagg cttcgccaat agctgaaatt
     1801 ggagcatgct tagaaaaaat gatggccgcc tgggaggggc gggggggcgc ccctatcacc
     1861 ccgccggctc aatcctcgat caggccagag cccctgaaga ccctcgccgc ctccgccagg
     1921 gtgaggtctc cgggggggac gatcacctcc gactcgacga tctccttggg gtccagaggc
     1981 tccccctttg ccttgatcgt cgcaatcagc ctcgatagct cttccttaag acccgcttcg
     2041 tcctccttcg cgacgcgatc gacgatgttg ttgtcgatga cgttgaggtc gtcgaggatg
     2101 gcggatgata cccggtactg cccctccccc atgatcctga tgatcatctc ccttcctcct
     2161 ctttcagcct tgcgaggtcc gcctcgacgc ttccgcgggc ccggacccgg gcgagctccc
     2221 tctcgacgtc gtctttgccc ccgaactcct ccagggtccc ccgctccacc agctcgtcga
     2281 gggctgcgga cctcgccctc atcgtctcga tcttatcctc ggacctctcg accgcgaggc
     2341 ctacgtccgc catctcctcg ctgatcccgg tgagggactc ggtcaccttc acctgggcct
     2401 cggcggcgga gtactgggcc tttatcgtct ctttcttcac tctgaaggcc tcgaccttgg
     2461 acgagagcct actctcggtc gcctcgagct tctcctgctc gtgctcgagg tcggcgatct
     2521 gctggtcgag gctcgccgtc tcgacggcca gggccgcctt cctctccagg gcctgccggg
     2581 ccaggtcctc ccggtcagcc ttcaccgcct cccgagcctg gtcatccagc tggtccatct
     2641 tcgaggcgag cctagccctc tgcagctcca gcctcttctt ggaggtggcg acctccgcca
     2701 ccccccgctt aacgttctgg aggagctcga gctgcttctg gtaagagtag tcgagggtct
     2761 cgttaggatc ctccacccga tccatcaggg tggtcatctt ggccctgacc acggtgctca
     2821 tccggttcaa caatcccatg ataatctccc cttctcagcc ggcatatcct ttcttcggtc
     2881 ccaggtccgg ggctgaggac gggcctggca ccctcaaggt gatgggacct tgaaggtcag
     2941 ctctttattc acctcgaact tcccgtcgag gaccgtcttg gtatcgatct tccgatggac
     3001 gtcgtttttg aaggcctgca gatcccacat cccggtgaac tcgcttttta cgtcgaaggt
     3061 ggtgcagttg ggggatatga ccatcctgtc gatccccgaa taggaggatg ctacgtttat
     3121 gttggatatg tccaggcccg taccgtggaa gacccgggag gaatcgaaat gctggacgtt
     3181 gacgagaagc ccgtccggaa actcgatctt ccggtcggtt tgagagaacc cgctcgactg
     3241 gttgaggctg ccggtggaga aatattctat cgaccccttc ccgaacaaga cctcggtgtt
     3301 ggtgaatccc ttccatcgat cctgatttga gcttacgatc tcggtctgcc cttctccgct
     3361 gacggtgagg tagccggtga cgaaaccgtc ctggaacgct ggcgaggctg aggctgtcgc
     3421 cgccaggagg aggatcggga ccagcaggat ggatagaagg gcgataccac tcttcatgtt
     3481 actcccacaa tatcataata cgctattaca tttaaacctt tttggtagat cgccccctgg
     3541 ctggggtagt atatcatccg ggcgaagggt ttggaggatt atggatagca tttatcacct
     3601 ttaacctcaa tagggcggcc gtgtatctgg actacttccc ttacgagtcc ctgaggccct
     3661 accaggatgc catgctcgac gacgtttacg ggacggtgag ggccggagat cacggggtct
     3721 tgatgatcga cgcccccacc gggagcggca agaccagctg catcgccgcg gccctggcgg
     3781 cagcccccgg caagatcgcg gtgacggtga ggacggtgag ccagattgac gtatacctcg
     3841 acgagatgga gaagatctgg tctaagacca gccaccgtcc caccataacc tacatggtcg
     3901 gcaagcagaa gatctgcccc atagctgacg agttctccgg ggagtctgtc tatgccggct
     3961 gcgccaggct ccgggaatgg acgaagagga ggatgatcgc gcggctcggg gaggtggggg
     4021 ggaagttcta cgacccctcc caggacagga acgcgcccca gagcgtcccc ggatacagga
     4081 ccttctgccc ctactacatg atgacgaggg aggtcttcga gctatcgggg aagccccact
     4141 tcaggcactc gggaaaggtc ctcgacctcg tggacgagct ccgggagggg atcaggtccc
     4201 cagaccggtt ccagaaggag tgtcgggagg tctgccccta cgaggtgatg agcctctccg
     4261 ccaagggtag cgacgtagtc atcctcaact actcccacct cttcagcccc gagttccagg
     4321 agatcatctt cgactggctg gagctggagc gagagtcgtt gaccctcttc gtcgacgagg
     4381 cccacaacct cggggacgcc gtgagggccc tcaatacgag gctcctcact cccggtactt
     4441 ctccacctcc ttctcggcca ggtcgatcat cctcggagtg aggagcctcg tattgaggg
//
LOCUS       DASM01000007            6406 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_91458, whole genome shotgun sequence.
ACCESSION   DASM01000007 DASM01000000
VERSION     DASM01000007.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_91458"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          923 a         2364 c         1889 g         1228 t
ORIGIN      
        1 cctcgtcctc gactaggatg ttgaactcct cgtctatgct gatcctgccg tactttcccg
       61 tcacctcctg gaggatcctg ccggcgctct cggcgatctc cctcctcatc ctcaccagga
      121 tcgcatccaa aaaccggttg accagaaccc tcgtcgtctc gacgacctgg acccgcctcc
      181 tcaaggcggc cgccctctcc tccagcaccc tcctcctggc agcctcgccc ctgagccttt
      241 cgaggttgcc ctcggcgagg ccgaggcgga ccatcccggc cgacgcctcc ttccgggcct
      301 cctccagcct gcgagaggcc tcctccaggg atgccctcgc ctcccggtac gcctcctcgg
      361 agaaggcgag ggccacgatc ccctcctccg cccgggcgac ctctccccgg agcctcgaag
      421 ccgccgccgc cgcctctccc cggtggcggc tcacctcccc ctccgccgcc agggcgagcc
      481 tgatctcgtt tgccgccgtc tcggcccccg cgagggacct cgcctcccca atgagcccct
      541 cgtactcggc gggatcgaag ccgagggcct ccatctcccc gtcgaccccc ttcagctcgt
      601 ctctgagccg ccgcacctcg gacctcaggc cgtccgccgc cgcctcgacc gccgggatct
      661 cccggattcg ctccgccagg gccgagaacc tccggtggac ctccctcagg gacccgatcc
      721 tctcccgggc ccggagcctc tcctcctcgg agtagccgag ggcctccatc tcctcggcca
      781 gggaggcgac cctggcgatg gacgtggcga gggcctccct cgccacctcc tcctcgctcc
      841 tcttcttcgg gaggtcctcg atccggaccg agagctgctg ctcctcctgg acgaggggga
      901 ggagggagga gaccttcgcc tccaggagcg agagccgatc tggatcgtag tcgacggggc
      961 cgatggagcc gatctccgcc tcgatctcct ccgcctcccg ggagagctcc tggccctggg
     1021 cgaggaggga ccgcctctcg gcgagaagcc ctccccgttc cgccttcaat tctgcgatcc
     1081 gggagagctc ccttctggtc ctctccatcg accgcttccc ctcctcgacc ctggcgagga
     1141 ggtcgccctt cataccttcg agggtggcga tcctctcttt tgccccggag gcggcggccc
     1201 tgtacttctc ctggaggagg ggcctctgct ccgcgagggg cctctcgcag gtggggcaga
     1261 ggctctcctc ccccagggcc tcgatcctgg acctctgagc ctccgcctcc cggagctccc
     1321 gcctcgccac ctccaggccg aacttgaggc cggcgagctc ctcctctgcc ccggccagaa
     1381 ggcggccgat cacctccccc ttcttgacga ggtccgcctc cctctcctcc aggtccccga
     1441 ggcttctgac ctccccctcg atcctttcca cctccgccct cagccgttct atccgagacc
     1501 ggacggcttg gtccctcatc tggaggccgg agaggagccg gtccttctcc ctctccctct
     1561 cctgccgacg gaccatctcc tggagggtcc tgaagatctc cagggacggg gtgatctcgg
     1621 cgatccggag cttcgcagcc gataagcccc agatctcctc ctcgaccctg gcgaggttct
     1681 cctccgccgc ctccttccgg gccctctccc gcccggcgag ggatgccagc tcctggaagc
     1741 ggtccctctt cgcctccatc ccctccagct ccgagaggag gcggcggtac tcctcctcct
     1801 ggggccggat cgcctcatgc tcccggctct catcccggag cctcgcgagc ctcgcctcag
     1861 aatccgagag gagacggccc cgcccctcca ggtctgcccc gacctgagcc ctccttccga
     1921 ggagggcctg gtgacgcggc ctcttcgcct ccatcccctc caggcggagc cggagctccc
     1981 ggaggcgcgt gagcttcggc tccagctcga agagcctcca cctcccctcc tcgatctcgg
     2041 agagccgcct atcgtccgag gcgatcgcct cctcctttga ggccaggtct gcccggagcc
     2101 gcgccacctc cccgacgatg gcgtcgcggg cccgcctccg ctccgcctcc ccctccagct
     2161 tctggcagcg cccctcgacg gcggtggcga tctcctcctc ccgccccttc gcctccagaa
     2221 gctcctccct cgccgaggcg acggccctct ctccctcctc gatgagatgg tcgacgtccc
     2281 cgatctcccc gagggcgccc tcgacccggc cgacctctcc ctccgcctcc ctcaggtcgg
     2341 ctcgtatctc ctcgagggcc ccgtcccgga cctcgtcgag ccccaggagg gtaaggaggt
     2401 attcccgctt ctccgacccc cggtctctta tcaggttgtc caggtcctnn gccgggcgta
     2461 gaaggtcttc atgaagtccg caaagcttat ctggaggatc tcggagaggc gggcgtccac
     2521 ctcccgggtc ccggtggcga cgagctcgcc atcgattctg agcctcgcct ccggcgtcat
     2581 ccccttcccc ctcatcgccc gcaggaccgt catctccttg ccgtcgtgct ggagggagag
     2641 ggtcacctcc aggtcgtccc gctctgaggc ccgggagttc ttgatgaagt cgcgcctcac
     2701 cgtcgaggcc ctgctcccgt agagggccca ggctatcgcc tcgacgatgg tgctcttccc
     2761 cgagccgttg ccgccgagga tgccggtgag gccgtcctga aactcgacct tcaccgcccc
     2821 ccgatacttc ttaaagttct tcatcgacag gctattgagg cgcatcgaac ctctccgtcc
     2881 tcctcctcgc agagatccgg gcgagaaggt cggtgccgta gccgaggacc tcctctctga
     2941 tcgccggggg gatggagcct ccggccgcct ccccctcgac gaacgtttcg aactcctggg
     3001 gcagatcctc cgccctcacc ggctccgtct cccggagggc gggctcgtcg ctgaactccg
     3061 ggatgatctc gaggtgaagg gcctcggccc tcagctcgcc aacgatacgc cggctgaggg
     3121 acctgtacgc ctccctcgag aggtctgtga ggcggagccg gacgatcttc tcctttatcc
     3181 ccccacggtg gcagacggcg gagatcccct cctccacctc ccgggaggag aggccgccgc
     3241 agtcgatcgg gtcgaggtcg agcatgtacc tctcctggac ggggatctgc tccaccctcc
     3301 cggattcgag gtctacgagg aggatcccct tctcgtcagc ggcctcgctg aacctgaagt
     3361 actcgacgga gccgctgtac caagcgttct tcgacacctg ggcctggccg tggtagtggc
     3421 cgagggcgat gtaggtgagg tcccccttca gaaagctctc ggcgatctcg tgctccccga
     3481 cggtccgcag cctcctgtcc cagagcgcct ccaccaggcc gtgcatcacc aggacgtccc
     3541 ttcctcccga catctccgcc ctgagctcct gcatctttct aaactcctcc atgtagtcct
     3601 cggggctgag gcagaagggg atgcagtgga agtggtgatc tccgacctcg aagcattcgt
     3661 gccggtagcg gtgagctatc tggaccccct ccatcccctc aaagaggctg aagggggaga
     3721 gggaggcgta gctcttgggg gcgtcgtggt ttccgctgat gatgacgacc tggattccgg
     3781 cctccaccag ccgctccagg ttctgctgga agacgtaaag cggcctgatc cgggggcgga
     3841 cgtggtggaa gacgtcgccg gcgtggacga cggcgtcggg ggagaggtcg attatcctat
     3901 cgacggtctt ggaaaatccc tggtagacca tctcctcccg gaggtttctc ccccaccggt
     3961 cgacccggcc gtaggcggag aagccgaggt gggagtctgc catgtggacg atcttcatga
     4021 acttccccca aagctaatcg gggggagggt tgaatccccc catcctcccc ctcccctcgg
     4081 aaggtccctc ccggttgagc cgttccaggt acttttcgta cagatggatg ctggcgggga
     4141 cggggaaggg gatcttcaga gtgctgatga tcgcctcccc cttctccagg gtctggatct
     4201 cgacgtccag agtcgacaga tcctgcttcg ccgactcctc caggcgggtc ctgtcgtttc
     4261 ggtcggcgag gccgaggatg acgaaggtgt tgaactggga gaggagctgc ttatcgatca
     4321 gcttcggctg ctgggttatg gcgcagaggc cgaccccgaa cttccgcccc tcccgggcga
     4381 tcgactcgaa cctggcgagg ctcccctcgc cgccgccgag gacccgctgc gcctcctcga
     4441 tcgttatgag gcagctcttc cccctcgccc cctcggcgct ctcttgcctg tacctttcta
     4501 agatcgcccg ggagaggatc gagaggagaa agagctcgct cctctccccc aggttcggaa
     4561 tgtccaccag gacgaccttt cccgcgagga ggctattgat gatcccgggg atgctggagg
     4621 tgaagtccga tatgtagcgg ttcttgtcca ggatcgtctt gatcgtcctc ccgatccttc
     4681 cgacggtctt ctcgtggaac ccctcggcga tcatcttcgc cgccccttcg ggggtgagga
     4741 actcctcgat ccacccctcc tcgaagaaga cccgggatat cgcctccagc gcctccctct
     4801 gggcggaggt ccaggcgtag aggacctcta tatcctgggg gaggatctcg ctcttcttca
     4861 ccgtcagctc cgagacggcc ggatcctttt tgtgccgagg atcggtggag tagcagacga
     4921 tcccatcccg gtaacgggcg aggtggagga gccccttggt cccctcgttg cccaggatat
     4981 actcgccgtg ggggtcgacg atgaggaggc cgaacttcga ctccttcgag gaggccttct
     5041 tcatgcacga tgcggcgaag accttcatga agttcgattt gcccatcccc gtcgtcgcaa
     5101 agacccccat gtggtggtcc atggcgaagc ttgcgagggc gacggggatc ccctcgacga
     5161 tctcgctccc gtttcggagg agccccacct cgacgtccgc ctcctcgtcg gcgaggaact
     5221 tgagctcctc cctcccggcc ttcgccgcca gggagaactt ggcggggatc gtcctcgcct
     5281 tccggaaccg ctcccctccc tcatccctca ccacgcagcc gaggggctct gccaccaccc
     5341 ggcagaagat ctggtcggag tcgtagaagc cgtcgccccg gatgacagtg tcccagtttc
     5401 cgtcgtagtt ggagtcgtgg aggatgtcgg tgatcctggc gaggaaggtg atctccgcct
     5461 ccctcctccc gtcccggatc gagaagatct cccccagctc caccacttcc tcgctcggga
     5521 cgatgaagtg gtactcccgg acgtcttttg atatgatcct gaagtagggg ttagagcttt
     5581 cgtccctggc tatggtctgg ggcttctgac cgattctggc gagaccctcc gggggggcgc
     5641 tctcctgccc cggggtttcc ttccaccttt catcagataa atctgccatt tctgccattc
     5701 tatctcctct tgatcgaatc gaggatctcg tgatagtcca gggcgccccg gacctctcca
     5761 tcagaaaatc ccagccccct gagctcctcg aagagggata gccgggtgaa gtctcgctcc
     5821 tggatcggga tcttcatctc ctgatgggcc ctgaagaggg cgtgggggta gccgaggctc
     5881 tccgaggagc gggagtagaa ggcgatccgc ccgaggatct cgtctattga ctccgccgcc
     5941 cggccggggg cgtcgatcct gaaggcgtgg gtcgatccgg ggtggagacg ggcggcgaag
     6001 gtctcgccac cccagggctc ctccctcatc gccctttggc cgctgagccg gacgagccag
     6061 gggctgccgg ggacggcggc gcacccgacg cgatcgacgc tcctgaggag gggccttgct
     6121 atgccgcgcc cccaggagag ctggctggac ttgctgatgg cgaggaggtc cacccccctc
     6181 tccccggccc tttcgaatat cctgtcgaag acctcccggt ggtacggctc cctccagagg
     6241 gagaagcccc catcgtagag gacgacgtcg ccggcctccg cctccaggac cgcctcgaag
     6301 agggcgacgt gctcctggag gtcccgatag aaggtcgcca tccgttcgag ctcctcggcc
     6361 gtcttcagcc ggaagggctc ctcgaggccg aagatccccc ggcgaa
//
LOCUS       DASM01000008           20584 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_67835, whole genome shotgun sequence.
ACCESSION   DASM01000008 DASM01000000
VERSION     DASM01000008.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 20584)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20584)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20584
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_67835"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         4209 a         6115 c         6482 g         3772 t
ORIGIN      
        1 gaggcggtgg cgagggcggc gatcgagtcg ggggtcgcaa ggatcgaggt cgatcctgcg
       61 gaggttgggc gaagggccgc cgagaggata gggctatcgg ggtagatgac agccacgagg
      121 gggcggtgaa ggcgggtacc cagagggctc acggccgtga taagggcagc aagcccctct
      181 aagtggtaac cccgcgcatg ctgaggcatt tccatgccgt tcgggcccta gtcttgggcg
      241 cccccctcaa cgatatccag ccccagctcc gccatgccga ccttaagaca acctcagtct
      301 atctttatag aaatcaaacg cctgcggatg atctgcgagg agttcgagat ctaaaggatc
      361 atctccaatc atatcataac atttgagcat agaaattgga tcttatgtgt cgctgccttt
      421 tggtcgccac atttgtaccc aagtggatat tcagggaccg ataaaaaatc ttggcgaaat
      481 atccactctt ggattatgta acccaaaaac ttttacgttt gtgtttccat acactctcac
      541 taggtgacta atatatgaga atagccattt tggtgttgat aacgctatca tccatcgcga
      601 tgcccgcgtg tgggcaggag actgctgaag aatggctaga ggaaggcata gctctctttt
      661 tccagggtag atactcatcc gcagttctgg cttacgacga ggctatacgt ctcgatcctg
      721 agtgggcggg gccctggaat aacaaaggca gttctcttaa taatttaggc aagtacgaag
      781 aggccatcca ggcttacgac gaagccatca ggctcgatcc tgggtgggca tggccctgga
      841 ataacaaagg cgtatctctg tataatttat ataagtatga agaggccatc caggcttacg
      901 acgaagccat caggctcgat cccgagtatt tcgaagcctg gtttaacaaa ggcaacgctc
      961 tcgttgatat gggtaagtac gaagaggcca tccaggctta cgacgaagcc atcaggctcg
     1021 attccgagtg ggcgttgccc tggaataaca aaggctattc tctcaataat aatggtaagt
     1081 acagagaggc catccaggct tacgacgagg ctatacgtct cgatcctgag tgggcgttgc
     1141 cctggaataa caaaggcatt tctcttaata atttagataa gtatgatgaa gccatccagg
     1201 cttacgacga agccatcatg ctcgatcctg ggtgggcatg gccctggaac aacaaaggcg
     1261 ttactctcga gaatcagggc aagctaactg aagctctcaa gtgcttcgac acggctatcc
     1321 ggatctatcc tgagtatgcc aatgcttggg agaacaaagg cgttgttctt gaggcgcttg
     1381 gcaggacgaa cgaagcaaac gcagctttcg cgaaggctag ggatcttata tctgatggtg
     1441 aatcaatgct aacgtcagcg gaaataccct cgtctccaat tacccttcca tcatattctc
     1501 aggtcgtaga aacatatcca gaaggtactg aactctgttg tctggaagca aatataactg
     1561 atcaaacgga agaaactggc ttgacactta gcaatataaa atgtcttcct cttttcggcg
     1621 gggacataca gtttcaatgc tatggtgcga agttaacttt ggatgttcca atcactatcg
     1681 acggaaaaag attcgatgta ggtgcaaaat tgaccgtgga caaagatcta cattggattc
     1741 aggtctctag ctgggactag actaagttaa aatggccgcg gatacaacaa cgctcggaaa
     1801 ctgaagatag aggcggcaaa gctccgagag atcccataga tcgaatatca gacaggggat
     1861 ggtaaaaagt atatagaata ggtccgcttg cggggggtga gttcccccgc accaatgatt
     1921 actggtatcc ccaattcaga gaggatgacc tccgaggacc tggaggctcg aagggcgagg
     1981 atcgagaaga ggaatgaggc gatccgagag gcgagggctc catcgtgggc cgtcaccttc
     2041 gccagtatag aaacatattt cgtggtcgga gacagtatct ttttcgccaa agggtgaata
     2101 tgacgagaaa gaaattgatt tcagaggacg ccgatgcctg gaataaaaag ggaggatctt
     2161 taaggatcca aggcaagtac gaagaggctc tccggctcga tcctgagtac gccctctatg
     2221 aactggggaa agcatattga ggcaattcgt gatatcgatg aatccgttag gctcggttac
     2281 gaacatacac cagtttggca ctgatcgatg agagggtgaa ggctcgcggt tctgaggggg
     2341 gcccctcttt tggctgagga gtttgggccg tgagagccgg gtgtacgcgc cagacctcca
     2401 catatactat agcctctgaa atagacgggt tcacaacggc atacctttag gcctccgaga
     2461 cgatagggga cacttatgaa atattcagct acaaaaatat aggatacttt ggtctgattt
     2521 atttaagatt aaaaggaagt atgttttata agatctccat cagtcagata tgggtctgcg
     2581 gaaaggtgaa cacagagggg aaaaaagaga aagagtggtc agatctcctt ctggacctcg
     2641 cccctgatgt cctcgaagtc gacgaagtgc atccccgaaa cctcccggag ctcccgggcg
     2701 atgaaccttt ttgtggacat gaccatgaac ctcttccccc tcgcccgcag gagccggagg
     2761 ggccgctcga agtcgccgtc gccgctgacc aggatcgcca tgtcgtagtt ctcgatggtg
     2821 ttgaacatgt ccaggacgat ctcgatgtcc aggttcgcct tcctgatctc ccggccgtcg
     2881 tcgaggacga tgatcttcag ctccttggtg atgatggagt agccgatgtc cgcgagggcg
     2941 gtcaggaacc cctgctgcct ggtgtctggc gggttctcct cccccacgta gtagtaggcg
     3001 tcgatgatct ccccgccggt cctgttctcg atccaccgga ggagcttctc gaagttgatc
     3061 caccacttaa gctcatcttt ctgcatgtaa aagacgttcg cgccatctac aaataccgat
     3121 attttcaatt atatttcccc tttattttaa atgtagtaac cgagacggcc gccgtcggcc
     3181 gcccatcgcc cttccgcgag gaagaatatc tgagaataca tatagtttcc tgaagtcccc
     3241 tgaaagaccc agacctccat agcccgcctc tggacggtat atcagtcgtt ggctgccgcc
     3301 cgggggggcg ggctcccctc cttctcgccg cagccgtcgc agatcccctt caggtccatg
     3361 ctggatccga tgacggtgaa gttcgcctcg gcagatacct tggagatcag ctctcccatg
     3421 atggggtcgg accaatcgat gaggcatccg caccggatgc acagcaggtg agcgtgagcc
     3481 tcagtattcg ggtcgtatct cgtctcgccc tgggagaggt tcagctccat gaccagcccc
     3541 atctccttca atattctaat tgtgctgtag accgtggcga ggctcaccgt tgggtgggtc
     3601 ttcttgacct caaggtatgc cttctgcgcc gttggatgtt cgtgattgct caggatatac
     3661 ctgctgacgg cgatcctctg gggcgtagcc ctataacccc tgttacggaa cgcctttatt
     3721 atcgatgcct caggatcgcc gtaattgatc atggtaatta ctaagtaata atcaatctta
     3781 aataagccta tgctcaataa agattttggg ggcaatcaac tcaaatcttg ggcgtccacg
     3841 ttgatcgagc cctcgatatt gatggatgat aaagagagga gatgctaaaa tgacccacca
     3901 tgcagaagac catttcggat gctgtgaagg gagcgctttc ccgttgatcg gagacaagtt
     3961 ccccatgatg aaggtgaaga ccacccacgg tatgatcaac ctcccggggg attataaggg
     4021 aaagtggatc gtactcttca gccatcctgc ggacttcacc cccgtctgca ccaccgagtt
     4081 tgtggccttt gagaagaggc gggaggagtt cgataagctg aactgtgagc tgatcggcct
     4141 ctccatcgac caggtcttct cccacatgaa gtggaccgag tggatccggg agaatatcaa
     4201 ggtggagatc aagttcccca tcatcgccga cgatcagggg aggatcgcgg agatgctggg
     4261 gatgatccat cccggcaagg gctccaacac cgtccgggca gtcttcatca tcgaccccga
     4321 ggcgacgatc cgcttgatcc tcttctaccc ccaggaggtg gggaggaaca tggacgagat
     4381 cgtccgggtt ctgaaggccc tccaggtcgt cgacgagaag aaggtggcag ctcccgccaa
     4441 ctggccgaac aacgagctga tcggagacca cgtcatcatc ccgccgccgg gggacgagaa
     4501 gacggcagcc acccgaaaga ccggcggcgg catagtctgc gaggactggt ggttctgcca
     4561 caagaagatc tgagatggcg ggaaaagtag ttaagaggga gaaccatcta taatggttaa
     4621 aggtgatggt atggcgaaga tcggtgttgt gagacatcgg gtggaggact tccccgcctg
     4681 gaggaagaag ttcgacgatc ggatggagat ccggaagagc aacggatggt cgggccacga
     4741 gctgtattat gacgaaggga agaaagaggc ctacgtcgtc cacacagtcc acgacgataa
     4801 gctggcgatg gcgaaggagc atatggagaa gttcaaggcc atgggaaggc ggacggagat
     4861 gaagccctcc ggaaaccccg accattcgat cgtgccgagg ggcgagaaga tcgaggcggt
     4921 aaattactga tcccgcgacg gaatggacga cccctcctcc gggccctggg gtcgccctcg
     4981 ccgcccagaa ggaggcttct ggggggcgcc gggccgcttc ctcatcctcc ggggtccgac
     5041 ccctccgccc tgcggccccg ggcgccaccc gccgatcgga atcagaggcc ggtcagcggc
     5101 cccccggcct ccggggtgcc cccgctccgc aaaaagttta aggaaatccg acaaggtttt
     5161 agaaaaacta tggagatatt cgcatggccc aaaagaaatt atactccctc cccgagctgc
     5221 cctacgccta caacgccctg gagcctcaga tctccgaggc ccagctccgg ctccaccacg
     5281 acaagcacca tgccgcctac gtcaacggcg cgaacgccat cctggagagg ctcgacaagg
     5341 cccgggaggc gggagccgac ctggatatga aggcgaccct caaggagctc tccttccagg
     5401 cgggaggcca cctcctccac agcctcttct ggaggaacct cgccccggcg gcgaaggcgg
     5461 ccgaggagcc gaaaggcgcc ctagccgacg ccctgaagga ggagttcggc tccttcgcca
     5521 ggttcaagaa ggagttctcc gccgccgccg cgaccgccga aggctccggc tgggcagccc
     5581 tcgcctggtg cggcatgacg aaacggcccg tcatcatgca gatcgagaag cataacgtca
     5641 acgtctaccc gatgttccgg atcctgatgg tcctggacgt ctgggagcac gcctactacc
     5701 tcgactacag gaacgaccgg gcgaagttcg tcgaggcctt ctggaagatc gtcgactggg
     5761 acgaggtgag ccggaggcta gaggcggcca ttaaataaga ccggtcccag gccggtcggc
     5821 tgccgaaact attttttggg gtacttgaga gcgtgaagat cctttttctg atcctgacga
     5881 cggtggccct cgtcgtcacc gccggctgcg tcgatcgggc cgaggagagg ggcggatccg
     5941 ccccaccact cccggtggag agctccaccg caccgatcga gagcggagat ctcacctatc
     6001 ccgcctacgt agcttccccc gctccaaagc aagacggagg cgacgatccg acctatcctg
     6061 ggatcgtgat gatccactcc ttcaacggcc tggagcccgg ctaccgggat ctggcagatc
     6121 ggctagcagc ctctgggcac gtggtgatcg cgcccgaatg gcagaccttc aacaggacgc
     6181 cccgggacga ggtcgtcgag gagctcgtca gaacctccgc tgcctacctc gtggataggg
     6241 aggacgtgga cggcgaccgc ctcggcctca ccggcttctg cgccgggggg aggtacacca
     6301 tgctatttct ccccagaatg gaggagtttc gctccggcgt agcctggtac ggatttccct
     6361 actccggcgg cttcaacgac gaggcccgcc ccgccgacct gatcgacggc ctcgccgcgc
     6421 cgatcctgat catccacggg acccgggacg tccccagccc catctcgggg atatacgatt
     6481 acgccgccgc cctcgacgcc gcaggcaagt acttcgagct gaaggtctac cagggggagg
     6541 gccacggctt catgatcaat gggggcgaga gagcggtggg cttcgccgcc cgagacgccg
     6601 aaggagagat ggcgaggttc ttcgagagga ccttgaagtg aggacggttc tgccttgccc
     6661 cccacccttc tttttgaatc ggagatgggc aaagaaaagt ttattgctgc ttaaatttta
     6721 ttgtgattat atgaggcgaa atctgtggat cggtgcggct gcggttctga tgctgttgtc
     6781 tttgcccgcg gcctttgccc agagctgcga ctggacggga acgtgggaga cgaactgggg
     6841 agagatggag ctgcaacagc atggcaacaa cacggtgacc ggaacttata ctcacgacaa
     6901 cggacggatc agcggaacga tattcggaga cgtgctcatc ggcacctggt cggaggacgc
     6961 ccaagaagat ccctatattc cgccctataa cgcgggagag gtcgagttca ccatctcgga
     7021 tgattgtagc tccttcgatg gtagatggcg gtatggctcc tctggagaat ggggaagctg
     7081 gtcaggcacg cgggttatgg agagatgaga ggaggatcag gcctctcgaa gaagttccgg
     7141 ccttgcgccc accacctcct tcggatcaga tctcctcctc tgaccggccc gtgaccatct
     7201 cggccaccca gtcccagtcg tggtcgtaga tgtgggccga gcagctcacc gtcgcgatgg
     7261 agccgggctc cgcccctgcc ctctccgcca cgtattcgag gagcctggtg aggccgtata
     7321 agttcgccgg gtaggcgccc gcgaagtcgt ggctccgaaa tagggccgtg aggtggagcc
     7381 tcccctgcct aattttaaag tcgtcgacca tcatgcaggg gacctcctcc ttctccgtat
     7441 cgacgggcgg gatccacgtc accgccgagg ccctccgcga gttcggattg gaccggaggc
     7501 ggcggacgac gtactcgatc tggtcgacgg cccctcccgc gccttcacca tcgataccca
     7561 tctcgccgcc ccgatcgtcg cggacccctc tctccttccc agggcagggg agagaccagg
     7621 accggagccg ctcgccgtag gtgtactcaa agccggggtt ctcgccactg atgagctgac
     7681 ttgcgtaatc ctccaggcgg ggccggttcc aggagtactc ctcggggatc atctcctttc
     7741 ttgggtcctc caccaccacc tccagggcca ggatctcccg ggtcctggtg cctcgctcgt
     7801 cggtgatctc ctctcccccc cgccatatca gccccagccc ccgccgccac gcctcgggga
     7861 tcgtctccgc cttgatgagc cttccgaccc tcacaagaac accctccgcc gttttggccc
     7921 aagggctgaa atagttggct cttgaagttg aaagggagag ctgaacttgg cagagctctg
     7981 gtgatccaag agatgcccga cttcgagtgg gagctggtgg cggcgataaa ccgcttcttc
     8041 gaggcgtccg ggattagggg attggcctac cggctgaagc agagcaggta caccccccag
     8101 gtcgtcgacc tcctcgtcga ctcccccgac cccgcctact acctggccat cgagtgcaag
     8161 agcatcgacg cccggaaggc taagaccctc tacttcaccc agcacttctc cgccgccggg
     8221 ggcgtccacc agatcgagag gatcgaccag ttcatcgata ggaccggccg gcggggcttt
     8281 ctggcggtgg agctccgccg cgggcccggg agggcgaaga ccgcccacct cgtcccctgg
     8341 aacgccgcga tggaggcctt caggtccgag agccctggaa tcccggtgga gaggatcgag
     8401 gcgatccccc ccctcggaag gagggatggc ggctacctca tcgaggcggg agatcttgat
     8461 caggaaattt agggccaatc aatttaactt cgcgcaagag aatttggatt aagcttatat
     8521 tcccttccgc cgctccaaaa ccccatgaag cttcccggta ccgcgaccca gccggagaac
     8581 gtccagatcg ccataggaaa gctcgacatc aggcccggca tgaccttcct cgatctgggg
     8641 tgcggctcgg gggccctatc cctcgctgcc agcaggttca ccgatcggat attcggcata
     8701 gaccggaggc ctgaggcggt ggagatctcc agggcgaggg tcccggcggg gaccttcctc
     8761 tgcggcgagg cggcggggat catccccggc cttcccaaga tcgaccggtg tttcatcggc
     8821 gggacggcgg gggtcgagga tttcttcccg atgctgatgg agaggctctc tccaggagcc
     8881 gccgtcgtcg ccgacctcgc caggatcggg gtcgcctcga gggtcgcagg gctgatgagg
     8941 ggggcgggga tcttcgagga gctcctccag atcgggatag cccggggcta cgacctcgcc
     9001 ggggacatcg ccctccggcc ggagaacccc atctttatgg tggtggggag gcttccggga
     9061 ggtcggagcc gatgagggga ggaggatcga ggatgagggg ccatgggagg ggacggcaga
     9121 tctcaggaga ggagagggag gtggcagcgt gctgataggg gtgagcctcg gcccggggga
     9181 cccggagctg atcacccgga aggcggagag ggccctggcg gccgcggata aggtcttcgt
     9241 ccccggagag atggccgccg acctggtccg gaggtacgcc gagccggagc tcctcgactt
     9301 tcccatgatc tcggataaaa ggaagctcct tgagatctgg gccgagaatg cggaccgggt
     9361 cgcctcctgg gccgccggcg gggctgccgc cttcgcctgc atcggggacg tcaacacctt
     9421 ctccaccttc tcccacctca agaggctggt aatcgagcgc cacccggagg tggagatcga
     9481 gacgatcccc ggggtgggga cggtccccgc cctggcggcg aggctcgggg tcgacctctc
     9541 ggagtcgttt ctggtctccg acggctcccc ggtgagtacg gtgatcagga tgaaggcggt
     9601 ccgccccgcc gagatggcta aagagctcgc agccgagggt ttcgacgagt tcctcctggg
     9661 ggtccgcctc tacgcccccg atgaggtggt cgtcagaggc gagatgccgg agaaaagcga
     9721 ctacttcagc gtcctctgcg ccaggaggag gagatgaagg tccacttcgt cggcgcgggg
     9781 ccgggggatc ccgacctgat aaccgtcagg ggcgcccggc tcctggcaga ggcggacctc
     9841 gtggtctacg ccggatccct ggtgaacccc gggctgctgg aggggctctc ggcagagctc
     9901 gtcgacagca acaagatcaa cctcgaggag atcaacggcc tcatgctctc cgccctccgg
     9961 aggggcgaga gggtggtgag gctccacagc ggcgaccccg ccctctacgg cgccatcctc
    10021 gagcagatga ggcccctgga ggaggcgggg gtggaggtgg aggtcgtccc cggagtctcg
    10081 tcattgttcg ccgtcgccgc cgccctcaag acccagctga ccctgaaggg ggtctccgag
    10141 tcgctgatcg tgacgaggcc agcggggacg accctcgcga gggactcgat ccgggagctc
    10201 tccaggttcg ggacgacgat ggcgatattc ctcggggcgg acaagatcag ggagacgacc
    10261 gagcggctcg aagtcccgaa acagacgccg gcggccgtcg tctaccacgc ctcctggccc
    10321 gatgagcagg tgatcgtcgg gaccgtcgag gatatcgccg accgggctga agaggcgggg
    10381 atcaccaaaa gcgccctcat cctcgtcggc gacgtcatat cgaggagcgg gtttgggagg
    10441 tctcacctat accgaagtcg gtagccgtcc tcgtcttccc tcggaacctc gccgaggggg
    10501 agaggatcgc tcgggcgatc ggagaccggt acgacccaga ggtcgtcgtc taccgccggg
    10561 agggacccga ggcgaccctg gaggccctcg gaaggttcga cctggtggtg gcggtgatgg
    10621 ccgtcgggat cgtggtgagg atcctctgcc cgtccctcac ggacaagtgg accgacgccc
    10681 ccgtcgtcgc cgtcgactcc tccctccgct ccgccgtccc ggtggtgggg ggccaccacg
    10741 gaggaaacga cctggcgtat catctatacg agaagctggg ggcgtaccct gccgtcacca
    10801 ccgccaccga cgcggccgga cggccatccc tggaggggac ggcggatcgg ctgggggctt
    10861 acgtcgtcaa caggtcctcc tcgaaggagg tcaacctcgc cttcctgcga gaggacgtcc
    10921 ccgtcctccg gatcgtgggg cccaaggtcg tcctggtcga cgagggggtg gcggtcctga
    10981 ggagcagggg cgggatcgtc gtcggcctcg gcgcaaggcg gggggtcgag gccttggagg
    11041 tgctggaggc ggtcaggtcc gccctggagg cggcggggcg gtccttcgga gagatccggg
    11101 cgatcgccac cgccgagatt aagagggacg agcccggcat ctccgaggcg gcggagaggc
    11161 tggggcggcc ggtcgtctac ctcgacgagg aggtcctcaa cgcccaatcc ccgacgacgg
    11221 agtcccgggc gagagacctc ggcctcgtcg gggtggcgga gcctgcggcc ctcgccctct
    11281 ccgagaggct gatcatgccg aagagggcct acgggagggt gaccgttgcc ctcggagagt
    11341 aggctttaca tcgtcgggat cggccccggc ggccgcggcc ttctcaccag gaaggcggag
    11401 aaggcgctct tggaggcgag aagcgtcgtc ggctacggcc cctacctcga ccttgtaaaa
    11461 gacctcctcc cgggaaaggt cgtccgctcc accgggatgg ggcgggaggt ggagagggtg
    11521 agggaggcgg tcgacctcct cgccgagggg gatgtggccc tcatcagcag cggcgaccct
    11581 aacgtctacg ggatggccgg gctcggcctg gaggtcgcct ccgggagggt ggagctcgaa
    11641 cgggtggagg tcgtccccgg gatcacctcc ttctcggcgg cggcctccag ggctgggatc
    11701 accttccggg aggcggtggc cgtcatcagc ctcagcgacc ttctcacccc ctgggagagg
    11761 atcgaggata gggcgcgggc ggcatcgcag ctggggatgc cgatggcgat ctataacccc
    11821 cggtccagga ggaggacctg gcagctggag cggctcctgg atatcgtcac cggtggcggc
    11881 gagggggacc ggcaccttct ggtggcaaag aacgtctccc gccccgggga ggccctctgg
    11941 tggacgacgg cgagagaggt tctggagagg gaggatctcc gggaagagat cgacatgttc
    12001 accctcctca tcatcggagg agaggggatg gcggaaggat ggcggtgggg tggggggaat
    12061 ggaaaggcgg aaggagggag aggggtggag gggatcgccg cgggagcggc cgtcgccgag
    12121 gcgaagatcc ggctggtggg ggtcggcccc ggagaccccc gccacctcac cctggaggcg
    12181 gaggggctga tccgggggtc tgacctcctc ctgggggccg aaaggcacct cgcctccgtc
    12241 ggagccctcc ccgggggcct tggggccaga tccctttcga tctcccccga ggggaggttc
    12301 gaggagagga tcggggcgag gctagcggcg gcggaggagg cggccggccg tggggagacc
    12361 gcatcgatcc tcttcggggg ggaccccggc accttcagct ccgcctggag gggcctctcc
    12421 cgcccgggga tccgggtatc tccgggggtc ggggccttcg cggcggcggc ggcgagggtc
    12481 ggagcccccc tggtgaacga cttcgccctc ctctcggggc tggacggaga ggcttcagag
    12541 aaggcccagc ggcttctgga ggccggcttc gccgtcgtct tctacaacca gagctccgga
    12601 gggctgaagg ccatcgccga ggccgcctcc tccctcgacc cggagagacc cttcgccctg
    12661 gtccaggacg ccacccggcc cgacgagaag atcgccatcg gcagggcctc ggatctggcg
    12721 aggccgaagt tcgatggcag aaggtgcacc ctcatccttc cggggccgac agccgatata
    12781 agagagggga ggatcatcgc ccgcagagga tacgagagga agtacgatta ctgatcgatc
    12841 aaaactggtg gcgttgaaga tggccgacaa aagatacgaa gacctggggg cggtgacccc
    12901 cgaggcgatg gagatctaca acaggagcag gagcttcatc gccgggatcg tgggggatag
    12961 gacccccgag gacaggatta agcagcggtg cgtcatcgcc accggcgacc tctccgtcgc
    13021 cgagatcctc aggttccagg gcgaccctgc cgccgccggc ctcgccgccc tggaggcggc
    13081 cgccgcgata ttcgtcgaca tcaggatggt ggaggcgggg gtgctgaagc gggggcacaa
    13141 tagctccatc atcaccgcca tcggcagggg cgatcggttg gctgagaggg aggggatcac
    13201 caggacctcg gcagggtttg tcgagctggc gggggaacta tccgggagca tcatcgtcat
    13261 aggaaacgcc ccctccgccc tcctgacagt ctgcgacctt atggagaggg gggaggcaaa
    13321 gcccgccctc gtcatcggcg tccccgtggg cttcgtcaac gccgccgaga gcaaggagag
    13381 gctccgcaag atcgccgtcc cctcgatatc gacggtaggc acccggggag gaactcccat
    13441 cgccgtcgcc gccataaacg agatcataaa catgcggcag gagaagaggg ggacctgagc
    13501 cctccggaga gggggggccg gggccccaaa gagggccagc aaagacggca gaggaagggg
    13561 ttcagggatg ggtgaggagg ggcagggaga ggaagggccg aaagaggcgg ggcagaagga
    13621 ggagatgccg gaagagaggg ggagagagga agggatgaga gatcccgtcg ccggcctatc
    13681 catcccccgg gactggatcg agaggtccgc cgacccccgg gtcctggaga aggtcagaag
    13741 cggcctctgg gccctcctct ccgacgggac cctcctccga agggggctca ccacggggac
    13801 gacggctgcc gccgccgcca agggggcggt catctccctt ggggggccgg tcttcacagt
    13861 ctcgatcctc accccggcgg ggatcagggt ctccgtcccc gtggaggggc gcagaggagc
    13921 ctgcaccgcc gtcaagatcg gaggcgacca cgcctccgac gtcaccgccg gggcggcgtt
    13981 gatggcgagg gcggaggcta tcggggccga ggcgggatat gaggcggccg ccggggagga
    14041 tcccggccct ttgggatcca actataccag gcacgtcgag gggaccgggc ccggagggga
    14101 ggggccggga aagaggatcg agctcgtccc gggggcgggg atcgggcgga tcggggctgc
    14161 ggggctaacc gcccccctgg gaaagccagc catcagcccc tcggcgaggg gagagataga
    14221 aacggccatc gaggagggga tggaagaggc cggcctggag gcggtccggg tggagcttga
    14281 ggtcataaac ggcgaggcga tcgcaagaga gaccctgaac cccaaggtcg gggtcctggg
    14341 cgggatctcg atcctcgggt cgacggggtt cgtcgagccc tggaacgagc acctcgccga
    14401 gagccgcgag gcggaggtcc tggaggcgga tcgggtggtc gtcaccaccg ggagggtcgg
    14461 cctcaggtac agccggttcc tatttccgaa atacagggtc gtcctcctgg gaaaccagct
    14521 cgatcggctc cggttcaggg aggggcagga ctccgtcctc tgcggccttc cggccctgat
    14581 cctgaagtgg gggatgcccg agctcctgga ggggacgggg taccggaccg tcgcggagat
    14641 ggtcgaggaa gagccgggcc acccccggat cgacgaggcg ctccagatgg tcggggagag
    14701 gctccccggg acgaggatcg tcctcatcaa cagggacggc tcgatactcc gggagcttcc
    14761 tccccccatc ccccatcagt cccgccgggc tcccgagggc cagctccgca aggccaaaaa
    14821 gtcgggttga aacaggataa actgaggtga ggtcagatga ggaggtctct gagatgaaga
    14881 tcgtcggcgt aggagcaggc cccgggatgc tgactttcga ggcggcagaa gcggttaaag
    14941 aggcccggct gatccggggt tcgaggaggg cgatcgagct ggtccgggag gtgatcgacc
    15001 ccggctgcga cgtcgcggag atcgaggact acagggccct ccgggagctg cccccggagg
    15061 cggtcgtcct ctccaccggt gaccccatgc tcagcggcct cggctacctc gaaggggagg
    15121 tgatccccgg gatatccagc attcaagtcg cctgcgccag gctcaaggtc agcctcctca
    15181 aggtcgtccc catcaccgtc cacgggagga ggatggaccc cgagccgatc gtcgaggagg
    15241 tggcccgggg gagggccgtc ttcctcctcg tcgacgacgc caccgacctt ccggggctat
    15301 gccgcctcct ggaggagagg gggctatcga gggatgtcgc cgtcctcacc gacctcggct
    15361 acccgaagga ggagatccgg cgggggagca ccagaagccc gcccccctcg agggggctcg
    15421 cctcggtgat gataggggat ctcggctttc accgttgagg tccgagaacc gctcctcggg
    15481 ccatggggac ggatggcggc cttctgacgc ggaggaggct ccgaccggcc tgagggttaa
    15541 gggcctccgc cgatatcgag gggtggatca ggggttggag ggctggatgt gggtcgggag
    15601 gggccaggat tatcaggttc aggacaaccc catcatttcc actggagatg ccgagatcag
    15661 gtctgataaa ggttggaaat ataaaaaaag ttcctaacct ggattgcaga ccttgcaggg
    15721 ggaatagccg gcctcccggg cctcccagac atcggagaag gttatcctgt tctctggctt
    15781 tatcttcttc gccgacttgc agtccggccg atgatacttg tcggaggtct tgctcccgac
    15841 gtactcgtat ccgagatcat ctgccggacc tgtcacggtc tcctcctcct cgtcgatggc
    15901 agtaaccgaa tcctgagatc tttcgtcccc ttcgagctcc tcggaggagg ctgcaaagcc
    15961 ccgagatcct tcagtggatc taaacgagaa ggactggatc cccgttgctg atccgtatct
    16021 tgaaaggggc tcgactgagg gcctctcgga gggcgcctcc cggatgggtt cctcggatcc
    16081 gccccagatc accctctggg gatcctcctc caccaccaga caatcccgaa gctccgctat
    16141 cgccaccccc tccatcgata ggaggccctc cggctcctcc acctccagaa gacaagaata
    16201 acccacctcg gggcggagac ggctgctctt tctgatgtcg aaatcccgcc ccgttcccgg
    16261 ctccagatcc atcatcatat acctggaccc ctcgacgagc tgatccccgt cccagagggt
    16321 ggcgttcagg ataaggtacg ggatcgtcga tccggtccgg acggccaccg agccccaggc
    16381 ccggaagagg gtgctgctga cggaggagtc gaagtccaag acctccaggg cctccgtcga
    16441 ggaagaggcg gccgacccag tgacgagact tcctgaaccc ggatcaccct cctccagccc
    16501 tggatcttct gcggccctca ggggggggcc gccatacgcc tcgctcctcc catgggggga
    16561 agatcgaagc tccttgatcc aaccccccca gtcctcctgc accgatgata gggcctccgc
    16621 caccggagag gggacaacga acccacctcc gaagtgaagg tacgccccgg aggcgaggac
    16681 ggcgcccagg agcagggtaa agataacagc ccttttccgg gatggaagcc tttttgtcag
    16741 ttgcataccc ccttatttcc actggagatc gaagggataa acgcctgcag gagagcggca
    16801 gggagaaaac caggtcaacc agtcctatcc gagccggtcg gacccgaagg ccgagccgag
    16861 acccctcagc cgctccagta cctccagcgc ctccttcaga cattccagct gacccttcag
    16921 cctgctcata tcctgctctt ccccgatctt atcggagatc tcccggatct tatcgaggat
    16981 cgccgcctct acccctccaa gggcgggatc catcggggat tctgccctca tacctcggga
    17041 taacccctcg cgacggccag attccccttc atcttcggct ttacgaggcc tctcctcccg
    17101 gatccgcccc ccttcctcag aatctgcccc aaagatcccc tccgtatctc cacgccgcct
    17161 cccggccggc cctgctgcgc ccttcggggg ggatccggcc ggagatccct ctccctgatg
    17221 ggagatctcc gccgccccca cctcttcaaa cgagcagacg gggcatacca cctcgccttt
    17281 ataacggaag agcggcgcgc cgcattcgtg atgggtggcc agcatcgtcc cacccatctc
    17341 caaaagccgc gtcatcctct tgatcgcctc ttcttcatcc atacagaacc acctcgatcc
    17401 ctctcttctc cggccctctt catcgcccac ctcgccagcc gaccctccag atcatcccga
    17461 tctagagctt tcgcctcgaa tgtcgacctc ccccgaccag gccagggcgg agatgcgccg
    17521 ccccttaaag acggctgccc tccagcgccg ccaggtcttt ccagcccgcc gagccccatc
    17581 tcagccctct gaaaccctcc tggaagtcgg gttccctccg gcatgccgcc accgactccc
    17641 cccggatcgc ctggggcttt ggggcgggtc gaagacccca gccacggaag aatcgccttc
    17701 ctatcgccga taaaccaggt cgtgaccacc atacgtttaa atatgactac catgtcatag
    17761 cttcatggga cggatttatc ctataacctt tttgccgtct tgaaagtgtg gtgattccat
    17821 gacagctgaa aacgtgatta agcaatgcgt cgaagtttta gagcgcatca tgagcgacga
    17881 taccgttccc agaaatataa ggcgttctgc ggatagcgtc aaggcgaccc ttctcaacgt
    17941 ccaggaatca gaagccataa gggcagcatc cgccatatct attctggacg agatcagcaa
    18001 cgatccaaat atacccctcc acaccagaac cctgatctgg aacgtcgcga gccagctcga
    18061 gacggtcccc gtcggatgag gccagatagc tcccgtatca accgacgcga tgggcgggga
    18121 tgaggggaac ggtggtgaca aataggccgc ctccaaggta agtcccacca tcagccttgc
    18181 attcttctca tccacctcga agaggaggat ccgggcatcc agagatcgaa cagctcaggc
    18241 ctctccgggg cttatgtgaa tttatgaatg ctatgcattt tgatctcctt tgagcctgtt
    18301 aagctcatta agcccataca gccccacaat caaccccggc cgaaggctgg tgccgaagaa
    18361 gaaggctcaa aggtggcggg ataagggaga tccgaccaat cagccctcct cgccccatcg
    18421 atcccgatga ccccctggct tccactggag ccccccgacc ccttggtttc cgctggaacc
    18481 gaggggaggt gatattgaga ggtgaatcga aagcctggaa aaaaaatatt aatatttttt
    18541 ctctattaga gatttatacc ctccagctac gaccctgata tttctactgg aacccccggc
    18601 cccctttatt tcgactggag atcaacggcc atgatcctat catcccggct ccggataatt
    18661 cgccataaat ggtgatgcaa aatcgtgaag atctggttta aaaaatcgat cggatcggca
    18721 gcgccccaaa gggcgaaacc ctccagagag aaatcatcgg ggcctatcca gatcgaaagg
    18781 cgcagccgaa cccgaatccc ccctctcatc ttttatctcg gatcatctct agagggaagg
    18841 atcagcatct ttaagtagga taagaaagct agttacagca ggatatattg acggcttttc
    18901 tctcttctct tcctggatat attccccatt ccagtggaaa caatggggtg agggggttgg
    18961 agtcgaaacg ggggtttcgc accgcaactc ttaataacag atagaatgta tttgcttttt
    19021 tgttcggggg gattgctctt tcatgtcagg cggattattt gcggacctat tgaagaaggg
    19081 ggagatattc gagtccagag aagtactgcg ttccagctat acgccctcgg ccctccccca
    19141 tcgcgaggat cagatcaaca gcatggcctc gattctggtc ccagcccttc ggggcgagac
    19201 cgcctctaac gtcctgattt atggcaagac ggggacgggg aagaccgccg tcgccaagta
    19261 cgtcggctcg gagctggaag ccgccggagc caagagcgcc tcaaactgct ctttcatata
    19321 catcaactgc gaggtgatcg atactcagta ccgggtcttg gcccacctgg cccgctgctt
    19381 tgggagggag gttcccatga ccggatggcc cacagatcag gtctacgagg aggtgagaaa
    19441 atccctcgac gaggaccggc ggatggtggt gatggtcctg gacgaggtcg acaagctgac
    19501 gaggaagggg gacgatatcc tctacaacct gacccggatc aactcggacc tggaccaggc
    19561 cggcgtcagc ctcatcggca tctccaacga cctgaagttc accgacttcc tcgacccgag
    19621 ggtgaagagc tccctggggg aggacgagat catcttcccc ccctacaacg cggagcagat
    19681 caaggacatc ctcgaacaga gggctacgat ggccttcaag ccgggagttc ttgccgagga
    19741 cgtcatcccc ctctgctcgg ccctggcggc tcaggaacac ggcgacgccc ggcgggccct
    19801 cgaccttctg agggtctctg gggagctggc ggagagggcg gggtcgaaca gggtgacgga
    19861 ggatcaggtg agggccgccc gggccaagat cgagagggat cgggtgaggg aggtggtgat
    19921 gacccttccg acccagtcga agctcgtcct ctacagcgtc ctcctcctgg aggagcaggg
    19981 gataaagaac gtcactactg gagcggtata cgggatgtac aagcagctct gccccctggt
    20041 ggacctcgat tttctcaccc accggaggat cgcagatctg atcgcggagc tcgacatgct
    20101 ggggatcatc cacaccgtgg tggtgagcaa ggggcgctac ggcaggacca aggagatctc
    20161 cctctccata cccctggcaa agctgaaggc gatgatcctc gacgactacc ggctcaagga
    20221 cctcatcggg ggggcggcct ccctccagag cagatggttc tagccgatgg ctctgaggta
    20281 agcgaacgga tgtagggctc cggccctcct ttctgccctc gaagatctga gaaatagatg
    20341 cgaagagtgc gccgaccggg attcgaaccc nnnnnncgtt ccagcgtaaa aacattgcgg
    20401 gcagaccggc ccccctccct tgatatatca gctatctccc tccgttagcc atcggctatc
    20461 ctccccttta tctgcacatc ttttatctat catcaactcg aaccgatatg gagttggtgg
    20521 tgtcctctct tgtacgacgt cataatcatc ggggcgggac cggccgggct caccgccggg
    20581 atgt
//
LOCUS       DASM01000009            5501 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_293494, whole genome shotgun sequence.
ACCESSION   DASM01000009 DASM01000000
VERSION     DASM01000009.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_293494"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          908 a         1834 c         1802 g          957 t
ORIGIN      
        1 ggagccggtc cccacgtcca ggaccctcat cccctctccg aggtcgagga ggtcgcacat
       61 gatcgccacc atgtgggggg cggatatcgt ctggccgtcg ccgatgggga gggggtggtc
      121 catgtaggcc atctgccgca cccgatcggg gacgaagagc tcccggggcg tatcttccat
      181 cgcctccagg acccggtcgc tgaccctccc ccggagcatc ctcagcatct cctccttcct
      241 cagagggcag ccccccagac ccggacgatc tccttggcga gggccctctc cggagatcgg
      301 ccgtcgcgaa ccctgatctt ctcgaccctg taccttcggc cgtcggcagt cctcacctcg
      361 ccgacggcga agggctcgtc ccccctggcg gggacgtcga cggatgtggt ccttccccgc
      421 ctctggaccg agaccttcac caccacctcg tccactgccc tcgtccagag ggtcatcacc
      481 tcccccgcct tcgccctctc cgtccgccgc ccctccgcca gctccaggga ggtcacctcg
      541 accatgacga cctctcccac cccgtcgtcc accaggatct cgtcgccgac ggcgaggagc
      601 tcctcctccg ggagctcgat cgtccggcgc gaggagctct cggtggcgct gacgatcacc
      661 ctgaggcctg tgagcctcgt cctctccagc tggtgggggt ggaccgtccc gcaggcctcg
      721 cacctgacga ggtgctcccg tcccagcttg acggttccgt gttcggtgac ttcgccacat
      781 tcggggcaga agatctcctc ttgcatggtt acaactcctc ctcgaaaaac tactaaaagg
      841 tgaccatccg aggccggccc tcgggccgga agggcggacc atggcccggg atcaccaccg
      901 ttgccctcct caaggcctcg atcctgctcc tcctcagggc ttcggaatcg gtggccagct
      961 catcctccag gtccagcagg ctgggaaggt cggtcctggg gacggccccg tcccgccacc
     1021 agaagaggtc ccccgccacc agccaggtct ccttccccga agggacgagg agggaggcgt
     1081 ggtcgggggc gtgtcccggc gtcgggagga cctctatctc cgtatccggg aggaacctgc
     1141 cgtaggggat ggcctcctcc ccccggtaga ggaagcttcc gtcgcagacc tcggcatccg
     1201 gaaagagcct gaggttgagg agatggtcga ggtggccgtg ggtgacgaat atgaggtcga
     1261 tctccgaagg cgatagcccc cgcccccgga gggcggatag gaggctcttc ccgtcggctc
     1321 cggggtccgc caggaccctc ctccccggct cctccaaaag gaccgtcgag ggcgatgccc
     1381 ggaacccccc gcccgccagg gtcacagcgt acccctccgc cagaaccgat atccaagcca
     1441 tctgctccac cccggaggat gcgatccctc ctcaaatgga tttaataagg tagaggataa
     1501 agatatgacg ggcggcccgt ttagtcgggc ctccgggagt tgggtggttt atgcagagtc
     1561 tcttcctctg gctggccctc ggggcggcgg tcctcctcgc cgtatcctcc accgggacgg
     1621 cgatggaggc gggccaggac ggcaaatcgt cgataacggt atcggagaag agcaccgtcc
     1681 accggccggt cctgaacatc tcagctgcct ccttcggagg cttcaccaag ataacggtta
     1741 tgagaaaccc ctctacgggg acggcgaccc gggagtccta cctttacggc gactccctcc
     1801 gccgggaggc gaggatgggg gtggacgagg acgggacggt gatggccgtc tcatccgagt
     1861 tcgacgggat ggcgagcctg ggggtggtca agctctcgac ccccaacgcc accccccaga
     1921 gcgcccccgc cttcctggcc tccgagtctt actatggaag cttcaagatc gacgagaagg
     1981 tggacgacta tggctcctcc atcgcctccg agcggtccgt ctcggggagg gggtatgtgg
     2041 cggtggataa gaaggtcggc gaccacctga ggacccacga ggcgggtacg ggctcttacg
     2101 cctccgaaga gcggatcgac gcctatacga gctacatctc caaggccgtc tccctctcct
     2161 cggagccgac gcccttcggg gccgccggga tcccggcgaa cctctccatc ccctggaggg
     2221 aggggatctc ctccagaaag gaggggaaaa gctacatcgg cgaggagtac tccggattga
     2281 cgaggctcga taagacgacg atcgtccggg gcctcgggga gatggagacg gaggcgaact
     2341 tctcggggac ggcccggtac cggctgatct cgtcggagga gctggagatg gacgaatcct
     2401 accgcggcga ctactccata tcgaggaacg tcgccctcca acagcctctg gtccgcgatc
     2461 cccacctctc ggtggcgaag gagggtacag tcgacgatga gggggagcgg accctcgccc
     2521 gctacgtcat caccctggag aacgacggcg gcaggaccct ggagccggtc ctcgtcaggg
     2581 acgtcttccc gccgggagca gtcttcgtcc ggtcgagcct gaggccgacc ctcacctccg
     2641 acggggccaa ctggtccctc acccacctct cgccggggga tgagagagag atagagctcg
     2701 tcctcgacgt gtcgaacttc cggggggagg agctggtcaa ccgggtcgtc gccttcggcg
     2761 gcctcgaagg ctcctggatt ccggccgcaa acttctccgg catcgagatc aactggctca
     2821 cctgctcttc gcccggcgcc gtctccatct ccaaggccgg agatgtggac gaggcggtcg
     2881 gaaaccgcgt ctggtacacc ctggagattg agaacctcgg agatcgcccc ctggtggcta
     2941 atgtgaccga ccggctcccc gaagggatga ggctgatcta ctcgtcgccg atcttcgcct
     3001 cctacgagga cggcgtcgcc acctggaacc tggtcgacct cgcccccgga gaggtcgaga
     3061 ccgtcgccta cggcgtcgag gctctctgga gcgggaggtt tgtcaaccta gccggggtcg
     3121 aggcgaggac tgcggacggc acgtctctcc aggcggcgac ggcgagatct gtcgtcgagg
     3181 tcgaggagtt cgagggggag cggccccgcc ccggctggca gccccccgcc tgggggctcg
     3241 gcggggcgga ggagatctgg ggatagagat gttcagagtg gagatccgag acgaggtgga
     3301 gatccggggc tcgccggagg aggtctggcg gattctcacg gacttcgagg cctacaggga
     3361 ctggaacccc ttcatcaggc cggtggtggg ggtggcgtcg gagggggcga ggctccgggt
     3421 ccagatcagg cctccgggcg gaagggcgat ggccttccgg ccgagggtga cgagggtcgc
     3481 gagaggccgg gagctccggt ggctggggag gctgtggctt ccgggcatcc tggatggaga
     3541 gcacatcttc gagatcgagc ccctgggccc cgaccgggtg aggctcgtcc agcgggagat
     3601 cttctccggc ctcctcgtac ccctggcgag aggccggatc cgggaggaga ccatcgccgg
     3661 cttcagggag atggaccggg ccctgaagga gagggccgag gcctctggat cgggctatgg
     3721 gggatcgccc ccttcagccc cgggggcgtg acggaccgga gcgtctctcg catctccgga
     3781 tcgggggtcc agaaccttta tatctaatca ggatgaaagg taaggcccgt ttggtgtatc
     3841 gaatggtcga tcattaccaa cccccttatt gctcccccat accggagggg gaggccctcc
     3901 tccccggaga gaggagttcc tacgtctgcc ggcactgcgg cgccaccccc ttcatggacg
     3961 ggtctcatca ccacgagagc tgccctcgat accggctctg aggtcggata tgccggaggg
     4021 tcccggagaa gcctgagaga agggtgccag gttgagatgt agaaagaccg cagtccttct
     4081 caaagagatc ttttggatgg attccgatta ttatatcccg tcagctgggc aggaaagatc
     4141 ctgaaaaatt gggcgggtga aggatttgga tgatccttct acccctccag ggagatcctc
     4201 cccccgcccc tcccgacgtt gtgcctgatc tccagggagt cggtggacga gatcgaccag
     4261 attatattga cggtctcccc cggccggaag gcctggtcga accggtcccc cgtctccatc
     4321 ctccgttcga actcgatcac cgtccagccg tcggcctccc ttcccccgaa gctcaggatg
     4381 tcgtcggtcc cgccgagctc ctcgtccggg gggtgggggc cgtagacccc cgtcgagtag
     4441 aggtcgagga ccgtagcctc acccccgctg acccagccca tcaccatgtc ggcgtccttc
     4501 atcgcctggg taggctcgaa gcctatcgcc acgaacccct cggcctggcc ggcgagcccc
     4561 atgtagaggg tctcgccatc gttcctccag ctgacgacgt accttccgcc ggacagggtg
     4621 aggttcttcg agtactcccc ctcggagatg acgccgtcag ctgcccatcc ggcggcctct
     4681 aaggggattt cgtggacggt gaggatggtg gagggggctt cttcggcgga gctcccggcg
     4741 acctgggcgg tgggcccagg gacggcttca tcttcatcgg cctcatcggt ctcgtcctct
     4801 cccgcggcgg cctccgcccc tcccccgagg cggaacgcca ccagcttggg gtcggccccg
     4861 accccggcgg gccagacgat cgtctcgccg gcgacggcgg gccagccgtt gatcccggcc
     4921 ggagccctca tcttccatac ctcctctccc gtctccgccc tgaaggcgtg gatggtgccg
     4981 tcgtactccg cggtgaagac gaggtcgttt atcaccgtcg ctccgccgag ggccatcgtc
     5041 tcgaaggacc tgatccagag gatcttgcct gtcgaggcgt cgatcgccac gagttctccg
     5101 gtcccgttgt tgaagtctat cgtcgacgtg tcgagggagg tcggcgtcca gtcggtgaac
     5161 atgttcacca cggggacgta gaggacgccg tcggagtagg ccatgggggt ctcaaccccg
     5221 ccgaggactc cggggaatat ccgggtcgtc ccgttcggca ggacgtccag ctgggcggcc
     5281 tcgtcgtacc tcccgacgac cgcggaccag aggacctccc cggagctgcg gttgaaggcg
     5341 tagaccttcc ccagcttccc tgcaccgatg accaccttct ggctcatccc cgcgaggacc
     5401 agctccgcca ggattggcga gctctggaag tcgtggtcga agaggtcgtg ggggaggacc
     5461 tgatggtacc agatcatctc gccggtggcg tgatcgaggg c
//
LOCUS       DASM01000010           14717 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_45639, whole genome shotgun sequence.
ACCESSION   DASM01000010 DASM01000000
VERSION     DASM01000010.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 14717)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14717)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14717
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_45639"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2712 a         4629 c         4479 g         2897 t
ORIGIN      
        1 tacgactccc tcctgaagga gaagacggtc ataaagaact acaggcccgc caccatgatg
       61 caccaccagg tcgagtacgc taaggcgatc gacaaggaga tcgacatcat cgtcaaggac
      121 aagcagtacg tctacgaaga tccggtctac cagggcgtcg gctacaccac catgaggatc
      181 aacgaggatg tcaccgacgg tatggtggac atcggagtcc tccagggcga gccggcctcg
      241 atggagcatc ccggttactt atatccagct acctccaagt gggtcaagcc gatgatcgag
      301 atcgacgagc actacgtcgg aaccttccac atcgagaaga acatgtccat cgacgtcccc
      361 tataagcatg aaagaacgcc gaaggactgg ctcagctgct gcataggtgg atgccccgat
      421 ccgtgcgagg tgggaacccc ctataacccc tgcgaggagt gcatggatat gacccccgcc
      481 gccaacaaga cgtcggcagg gtcggtcttt gcctctgcgt acacccccac caccagcggt
      541 ggatgctgcg gcaactgaag gggaagatct tcccccccct cttttcttct ttttgatccg
      601 gtcgggtcat tcgtctcaat tgccgattct atcgccccgg ccgaaaaggc ttaaccgtcc
      661 ccggaggatg gcgttaccat gcagttctcg gagtgggagc cgatctacgc ggagatcctg
      721 gcggagttcg gcttctccag ggaggaggac gagggggcgg cccggctcct ccagagcctc
      781 cttctgggcc gcgagggcga cctctccata aaggagctcg cctcgatgat ccgcgggagg
      841 agggtcctgg tctgcgggaa cgggccgagc ctcccgaaag gcctggcgga ggccctggga
      901 gggggagaag ggtcgccgga gccgttgaag cggagcgatc tcgtcatcat agccgccgac
      961 ggggcgacga cccctatcct cgcggccggg gccctcccgg aggtcatcgt caccgacctc
     1021 gacggctttc tccccgacat cctcgcctca aaccgccggg gttcggcgac ggtcgtccat
     1081 gcccacgggg acaacctggc ggccctgaaa gagtatgtcc ccaggctcac cagagtcctc
     1141 gccaccaccc agtcgcgccc cctggagggg gtatacaact tcggcggctt caccgacggg
     1201 gatcgggcgg tcttcctcgc ccgacggtta ggggccgggg agatccatct ggtgggattc
     1261 gacttcgacg accccaccgt tacggcgaag aagaagaaga agctggcttg ggcgaggcgg
     1321 ctggtggcga tggccctgga gggcaggccg gatcccgccg attaggtggc cagagcccca
     1381 tttctcaccg gcaccttggg ccttcccccg tctttggagg ccgttgccgg ctcctggaag
     1441 gcttatcgga gtcctccccg atcttattgg cctttccggc cctcagcctt cggcgaggac
     1501 ctcggcgagg gcctccgccg tctcggcgag gacctcggcg atggggcggg aggcgtcgat
     1561 gacgacgaac ctccggggct ccagccgggc cagggtcttg aagttctctg ccaccacccg
     1621 gaggaactct tcccgctcga acttctcggg ccccttcggc gccgaggggc aggcctctcc
     1681 ccgggagagg cacctatcca ccgccagggc ggggtcgatg gagaagagga tcgtcctatc
     1741 ggggacgacg ctccaggggc ggtggagctc tcgcacccgc tccatagggt cagagaagac
     1801 ctcccggagg gtggcgccct ggtaggcagc ccgggagtcg gagtaccggt cggataccac
     1861 gaccatcccc gcctccaggg cggggatcac cgtctccgcc aggtggcggg cgtggtcggc
     1921 catgaagagg aagagctcct ccatctgccc cgccctcatc gccgccgccc cgacgcctcc
     1981 ggccgccggc cccctcagcc tctctcgcag aagccgcccc tcttctcctt ccgtcggctc
     2041 ggcggtgaag agaaatcgcc tcccttccac ggccagcttc ccttctgatg ccatgcgggc
     2101 gacgccggcg agggccgtcg acttgccgct accgtctatc ccctcgaagg tgatcagaac
     2161 tcccctcata atcagcatct tccatcaagg ggtgctgata tttaaggatg gggaagatcc
     2221 cgggataagt ccctcgtaag aaaaatcgaa gaattacacc aaaaggcgac tctacgccca
     2281 tggatgtgga gatgtcgccc gatgcctgct aactctgagg cctcatcctt tggaagatcc
     2341 gcaagtcggc tcagtgaccc tcgcagcggg cttattgtac cgcatctggg ttgtcttgag
     2401 gtcatcgtca gacgatagtc aatatctccc ccaaggacct atcaacgcgg cttttcgtgt
     2461 cgctccccct ttgcgccggc agtcacggcg acggcggatg cctaggctcc tgccagtcct
     2521 ctgaggggaa ccctcgatat ataagcaaag tactatataa agatgcctgc ctccctcgat
     2581 cccggctccc cctcccccgg ggttgtaaca taatttcggg ataaacgcct cgtggcaaag
     2641 gtttaagtcg aagtcgcgcg ttgattatct tcggaggtgt ttcatctggc tgataacgtc
     2701 tacgtggttg gccacaaaag tccagacact gactctgtag ctgcggctat atcctacgcg
     2761 aacctgaaga accagctcgg aactccggag atatacgtgc cggcggcggc gggaacgatc
     2821 aacagcgaga cgaagttcgt cctcgaccac ttcagcgtcc cggtgccgga gacgatcacc
     2881 gacggcaagg ggaagaagat cgtcctggta gaccacaacg aggtcggaca ggcggtggag
     2941 aacatcaagg aggcgacgat cttcgaggtc atagaccacc acaagatcgg cgacatcacg
     3001 acccctaacc ccatcttctt ccacaacgag cccgtcggca gcaccgcgac gatagtctcg
     3061 gcgatgtacg accagtacaa ggtccccatc tccaaggaga tggcgggagt gatgatgtct
     3121 gccatcctca gcgacaccgt cctcttcaag tcccccacct gcaccgggaa ggacaaggat
     3181 gaggtggcga agctcgccaa gatcaccggc gaggacttcc agcagtatgg gatcgcgatg
     3241 ctcaccgcga agagcgacat ctccaccaag accgccatgg agatcgtgaa gggcgactac
     3301 aagcacttcg acttcgccgg gacgaaggcc ggcgtcggcc agatcgaggt gatggacctc
     3361 tcggtcctcg cccccaggaa gaaggagatc ctggagacga tggagaaggt gaggaaggac
     3421 gaggggctca gcttcgtcct gatcatgctc accgatgtga tgaaggaggc gagcgacctc
     3481 ctcttcgtcg gatcccccgt cgataagttc gagaaggcct tcgaagggaa gatggagaac
     3541 aacgccatct acaagaaggg ggtcttgtcg aggaagaagc aggtcatccc gcccctggaa
     3601 gcggccttca agtgagggca ggcttccgcc atttttatat tttcatctgc tcggatgaac
     3661 ccgcgccccc ctcatcctca ccaggggctt cgataggtgc ctcctggcag aaggaggaac
     3721 ctcgtagaac cccagctcga tctctctatg gatcggagcc tccaccttcc ccccggccct
     3781 ggtcctgcac ctcctgcacc ggtaaccttg accccgccct gccgacttca tccgccgtct
     3841 gcaggagggg cagaggggcg ccgtcggggc cttcacctcg gcgagggcca ccacctccat
     3901 cttctcgagg ttcagggtcc ccctcttgac ggcgccgtag acctcgatca gatctccggg
     3961 gaggagggcc ctcactcgat ccctgaagtt cttcgtcggc tcgaaggcgg cgcagttgat
     4021 ggcgctattg ccgtcctcgg cctccagggg gaagaagatg tgccccccct cgatcggctt
     4081 cgcacccccg gcgaccacgc cccggagccg atagctcccg tcctcggcag cctccgagat
     4141 cttgcaccgt aggatgtggg cgtcggtgcc ctggtttgtc ctgtagatga ccgccctctc
     4201 gtatgcctcc gatctgatcg cctccagggc ctccctgagg ggctccggcc cctccccccg
     4261 gatcccgaag aggacggggt ccccggagtg gggggcgaat accacctttt ggttgtggtg
     4321 gtcgacggtg tcccaggtcc gcgggtaggt cagctcgtcg gccctccaga ccgaccccgc
     4381 gtcgatctcc cggggcgtgc cccaccgctc gggccgccta taggcgagga gctcataggt
     4441 ccagtcggag aactccgccc cgacggcggc gagggccccg atcagccccc ggcggttctt
     4501 gaaggcccgg gaccagaggc cgtgggcctc gatgatctct tccgcctcgc cgacctccag
     4561 aacctcgaag agcgccctcc ggtagaagga ggagagatcc gccggcacct cctccgccac
     4621 caccaggccg gggttggtat tggggcccga gaagtcggag agctccagga cggtctggag
     4681 ggcgacctcc ttcgcctcct ccggctggtc ggtctccagg gagaaggcga cggcggcgtt
     4741 ccccctcgtc ttgaacttga cggcagggtt gagcctcacg agcctgggga gcccccggac
     4801 ctcaccgatc tccccgagcc tcgccatcat ggtggcggcg aggtaggtgg tgcagaggcc
     4861 gtattctgaa tcggtatcgt ctacgcctat ggtgatcatg atggtgaacc ttaaataacg
     4921 gctgagccca aagatatacg acgatgaata aggagtttct gaccgaccaa gtcagcaatc
     4981 ttctgtttag ggcgggattc tccgtctccg accggtgtta cgtcaggccc aggagcttcg
     5041 acctcgctgc cagacggggg ggcgtcctcc ttattctcaa gatcctctcc aacatcgacg
     5101 gcctcaacga gaagaccgcg gtggagattc gccgcctcgc cagatacctt tcggcaaacc
     5161 ccctcctcgt cggggagaag acgagggacc acggcctgga ggggggggtg gtctacttca
     5221 ggtacggggt tcccaccgtc agcttcagca ccctcgccga ctggatcgtc gaggatatcc
     5281 cccccctcgt ctacgccgcc cacggggggc tctacgtcaa gatcgacggc cgcctcctga
     5341 ggaagctcag gctggatagc ggcaaatccc tgggggccct cgcctcggag ctctccgtct
     5401 cccggaggac ggtcagcaag tacgagatcg agagcatgga caccaccatc gatgtcgcca
     5461 tcaggcttga ggagctcttc ggccaggagc tgataaagcc cgtcgagatc ctggagcccg
     5521 ccgcccccgt ctccagagag ggggggcgga tcgacgacaa ggtcctcagc cacctcgccg
     5581 atatcggctt cgacgtcttc cccaccgccc agtccccctt caacgccatc acccagaagg
     5641 aggatctggt cgtcctcacc ggcgtcggca agttctcgtc gacgatgctg aagaaggccc
     5701 ggctgatgag cagcctctcc atcgtcacca agaccttctc ggcggtgatc gtcgacggcg
     5761 agtcgaaggt ggagcgggtg gaggagacgg cggtgatcga gagccgggag ctggagcgga
     5821 tcggcaagac gagcgagttt gcagacctca tgtcggagaa gcaggataaa tgactattcc
     5881 ttatctcccc atcctcccat ggacccccgg tccgggatca ccgttggatg ggatctcgtg
     5941 atgggtgatt tttgtggcat ccagtttggc tgagaggctg aaggggtcgg gccgggtctt
     6001 cgtggtggcg gtggcggggg acagcggctc cggaaagacc accctgtccc gggggatccg
     6061 gaggctcctc ggcgaggagg cggtctccac cttcagcgtg gacgactacc acagcttcga
     6121 ccgggaggcg cggcgggttc gggggatgac ccccctgaac ccggaggcga accgcctcga
     6181 cctcctcgcc gagcacctcc ggtccttgcg gaagggcgag actatcgcaa agcccgtcta
     6241 cgaccactcg gtgggagaat ttcgggaacc cgtccccttc cggtcggggc cagtcctgat
     6301 cgtcgagggg ctccaccctc tttacaccca gaagctccgt cgggaggtcg actttgggat
     6361 cttcgtcgac ccgagccggg cggtcaagag gcggtggaag atcaggaggg actgcggcga
     6421 taggggctac cagcgggatc tggtgatggc cgagatcctc gcccgggagc cggactacaa
     6481 gctctacgtc gacgtccaga aggtctacgc cgaggcggtc gtcaagatcg acgatacgcg
     6541 gcttggggcg ccctccttcc tggaggaccc cggcgagagg tactccgtca ggctgatcca
     6601 ggaggttctg ggggccgggc cgtcggcggt ggacctgaaa atcgacctct ccgccctctt
     6661 cagcctccct gagggggagt tctccatatc cttccaaagg gacgactact acggcaagag
     6721 ggtgagcgtc ctgaccgtcg acggcgagct ccaccgctcg atggtggagg gggtggaggc
     6781 gaagctcaag agcctcgccg gctcgacggc gccgatctgc gaccgggccg gaggcgaccg
     6841 ggtcacctcc accgggatgg cacagctcct cctctcctgg cggctcctgg agaagcttca
     6901 gctccatctg gatacggctt agagaccgcc ggccgcctca gtcgatcctc ctgaacctat
     6961 cccccttgac gccgcagaca ggacacctct cgggaggctc ccgctccgcc gtcatgccgc
     7021 agacgggaca gacgtagtag gggaagctct cgtccttccg gggctcgagg ctctccatca
     7081 tctcgtggta gagccgcgcg tggatctcct cgacgtcgtt ggcgtatcgg aaggtcctct
     7141 cggcggcccg gtccccttcg gcctgggcgg ctccgatcat cccggggtac atctccttga
     7201 actcgtgggt ctctcccgcg atcgcctccc tcaggttctc cttcgtcgtc ttgacggcgt
     7261 tcatcgcccg gaggtgattt attgcatgga ccgcctccgc ctcggcggcg gcgcgaaaga
     7321 gcctggcagc ctgggggtag ccctcctggt ccgccttctc ggcgaaagcg aggtacttgc
     7381 ggttcgcctg cgcctccccg gcgaaggcct ccttcagggc atcttctgag ttggacatgt
     7441 cggcggttta tcgagctgat ttgataaaaa gagattggaa ggtcccctcc ccaggctcct
     7501 tatcaggtta aatctttccc ttattccgtc ccggatgttc ctgcagttcc tggccttcct
     7561 ccagtctcag gtgctttttc cgtcgtctgg gatcctgggg gggcggatgc gccctcttgg
     7621 ccggttctgc cgccgaaata gaaccctatg accgaggaca aggctcccga gaggatcagc
     7681 agaatttcct tgacgatatc tttgtccccc tccgaggcgt tgacgaggag gaagactacg
     7741 gcgactccca cgatcacgat cacgccgatg gccatggctg cccttgccac ccctccaacg
     7801 ccgatcggct ccatggatat gaactctttg atcagacttt ggttttcatc ttgactagtg
     7861 ctgccattct tggccaacaa tttttccaaa atatcctgcc gttttccgta atagtgatac
     7921 gctccgagga cgtttacgaa gactatcgag aaggggacga gagctatgac catcactatg
     7981 aatatcagcg tcccactccc cggcttactc caatccggga tttcttctcc ttcgacacct
     8041 gggtcgccag ccgttgtatt ctcatcgatc ccttcttctc ccgtcaaatc gccccctgga
     8101 ccgctgacgg cgttcccttg gtcagtcgct tcttcttggg gggcagaggt atggtcatcc
     8161 gtggctccgt ggacggatag agccagaagg acgatcaaaa ccagagccgc gaagcttttt
     8221 ctatatattg aggccaccat cgcctttcac ctctcataca cagttgattt ttatgtataa
     8281 tcaagttttc gattaagttt ctaacctcgc ctttgaagac cgtcagctca aaactcttta
     8341 ttacagggtc agccacagtt acaatcatgc tgacgatcga tgaggggagg cgggccgtcc
     8401 atctcgcgag ggaggccctg acggcttacg ttgatcggag ggagaggata gctccggagg
     8461 atctccctcc agccttcagc gagaaggccg gggtcttcgt caccctccac aagggtgggg
     8521 agctccgggg ctgcatcggc tacccggagc cggtgatgga tctgggggtt gccatagtcg
     8581 actcggcgat caacgccggg gtgaacgacc cgaggtttcc ccgggtcagg ggagaagagc
     8641 tttcgcggat cgagatcgag gtgacggtcc tctcgccgcc gaggccctac tccgtaagaa
     8701 atagagatct accgggggcg gtccaggtag ggcgccacgg cctcatcgtc cggcggggga
     8761 tctggtcggg gctcctcctc ccccaggtgg cgacggagtg gggcttcgac gcgacggact
     8821 ttctatgcca gacctgcatc aaggcgggcc tcccccccga ctcctggttc gacgaggaga
     8881 cggaggtcct cttcttcgag gggcaggcct tcgccgaggc ctcccccgga ggcgcggtgg
     8941 tggagtggga tcttgagggc tgctcctgag gccccaagat ctcggaggag ccttctagcc
     9001 ccctctctct ttcgtcttcc tcgtcgcctt ctccgcttcc cgctccatcg cctcccgggc
     9061 gagcctgatg gcgcagagct cgttgcacat ggtgcaggtg tcgacctcga cgcctcttcg
     9121 gtggcggatc tctcgagcga tggcggggtt tatggcgagg tcgaactggg agggccagtc
     9181 caggtcccgt cgggctgcgg ccatcttccg gtcccagagg cgggctctat ctttgacccc
     9241 cgccttcgcc aggtccgcag cgtgggcggc gatcctggtg acgatcgtcc cctcgaagat
     9301 gtcctctttg gtggggaggt ccaggtgctc gctgggggtc gtcgcgcaga ggaagtcggc
     9361 gccgctcatc ccggcgatca cccccccgat ggcggcggtg atgtggtcgt atccgggggc
     9421 tacgtcggtg acgatcggcc cgaggaggta gaggggggcg ccgtcgctga cgtacttcat
     9481 cgcctttacg ctcgtctcga tctcgtcggc ggggacgtgc cccggcccct ccaccatcgc
     9541 ctggaccccc gccctcctcg cccgatcgac gagctccccg aggatgatga actccatgta
     9601 cttcgcccgg tcgctggcgt cctcgatgca gccggggcgg aggccgtcgc ccaaactcaa
     9661 tgtcagctcg tgctcccggg ctatgtccag gaggtggtcg aactcggcat agtaggggtt
     9721 ctcctccccc gtctcgctca tgtacttgag ggtgagggat cccccgcggc tgacgacgcc
     9781 catgatccgg tggctcctct tcagccgctc gaaggagttc aagttgaccc cgacgtggac
     9841 ggtgacgaag tcgacgccgt cctccgcctg ccgctccagg gcgtcgaaga gcccctggct
     9901 cgtcagatct cccgccacct ccgcctggta tatggggacg gtcccgacgg gtacggctac
     9961 ggcatcgaga acccgccgcc ttatctcgtc caggtcgccg ccggtcgaga ggtccatcac
    10021 ggtgtcggcc cccgcctcca tcgccacgac ggccttctcc acctcggcgg cggggtcggc
    10081 gaccccccgg gacgagccga tattgaggtt caccttggtg gtgagggtct cgccgatccc
    10141 ggtcggtctg atatcgcgcc gggcgttcct cgggatcacc accctccccc gggctacaag
    10201 gcgggtgagg gcggcggggt cgaccccctc cttccgggcg acggcctcga cctcgggggg
    10261 ggtctttccc ttcttagcgt cttcgatgag agtcatgata ccaactaacc gatatattta
    10321 aggaaaccca tccatcttat gaggtgtttt gaatggtgga agtgcataag gttaacggcg
    10381 gcggcccggc ccacgagggg aacgtatacc tgatcatcga cgaggtggcg gccctcatcg
    10441 acgccgggat ggacgccgag gcgacgatga ggaacgtcga gaagctcgtc gatcccggag
    10501 agatcgagtt catcatcctc acccactgtc accacgacca caccgccggg gttccggccc
    10561 tcaaggcggc gaccggcgcg aaggtcctga tcggggaggg ggaggtctgc gacctcggcg
    10621 acgacgtcgc caccggctcc taccagtacg gagatgaccc ggcggagttt ggaatcgacg
    10681 ggaccctgga ggagggggac gtgatcaacc tcggagagtg ggcgctctcg gtcatcggaa
    10741 ccccgggcca ctctcccggc tccatctctt tatacgagcc gagggcgcgg gtcctcttct
    10801 ccggggacac cctcctcccc cacggaaaca tcgggaggac ccgccggtcg ggggagatgg
    10861 tcagagagct ggtcaggtct gtggagaggc tggcgtccct ggaggtccag gtcctctacc
    10921 ccggccacat ggagccgacg gacgaggacg tcggcgagca gatcaaaacg agcctggagt
    10981 tcgcccggtc gatccactga tccccccgga ccgccgccta tttttatcat cggcctcgag
    11041 gggaggcgat gctcgacgac atcttcgacc tcgtctacct catcgggttt ggggcggcct
    11101 ccgtcatccg gctcctttgg cttcggagga ccgatcactg gtggcggagg cgggagatgg
    11161 tggctttaga ccgggagagt agacaggacc ggcccctcat gctcctcacg gccgccggga
    11221 tgatcgtcgt ccccttcctc tacctcttca cgacgaggct cgacttcgcc gactacaccc
    11281 tgccgcccac attgagcctc gtggcgggtt ccctcggcgc cgccctcttc ctccaggccc
    11341 tcatccttct gtggcggtcc cacgccgacc tcgcgaagag cttctcgccg gggctccaga
    11401 tccgaaaggg tcaccgcctc gtcaccaccg gcgtctacga gcggatccgc caccccatgt
    11461 acgccgccca cctcctctgg gggacggctc agctcctcct cctccagaac gcgatcgccg
    11521 gccccgcctt cctggtggcg tcccttcctc tatacgccgc ccggatcccc cgggaggagg
    11581 agatgatgct ggatcaattc ggcgaggagt accggcggta tgcggagcgg acggggcggg
    11641 tggtaccgcg gtggcgggga tagccctcgc cggggggagt taggagggtt gattcttctc
    11701 tttcgggttc gagaaataga tacgaaccgc cgatcagctg gacaaaagga ctttccaaat
    11761 taggccaggc cggtgggtgt tgtcatcgag tcgctataat gcgatgttga acgaggtgtt
    11821 gaaggatgcc cgagcttcca gaggttgaga tttaccggag ccgtctcgcc cataccgcac
    11881 tgcataaaac gatcatcggc gtggaggctg attttgaaaa ttatgggagg attttccaga
    11941 ccccgaaaag ggcgctcgcc tcccttgaag gagagtcctt tgagagggct ctccggcacg
    12001 ggaaatactg cctcctggag gccagcgggg acaaggttct ggtattgcac ttcgggatga
    12061 ccggcaacgt gaaatattat aaaaaagaag attccgatcc ggagcattcg gcgatggtcc
    12121 tctccttcca ggatggctgc aagctggcca taattagcat aaggaagttt ggaaaggtca
    12181 ttctggcgga gagcagggag gagttcgttg aaaagcaggg gcttggcccg gatgcgcttt
    12241 ctctggacca gacggggttt gttgagacga tggaggcgaa gagggggcgg atcaagtccg
    12301 ccctgatgga tcagagaacg atctcgggaa tcgggaacat atattcggac gagatcctct
    12361 atcatgccgg gatcaggcca gacagaaagt tggacggtct gggggaagtg gatctggaaa
    12421 ccatctatga aaaaatgatc tccatcctga ataccgctat cgaaaaagaa gttgattatg
    12481 agaagattcc tgaaaattat ttgataaagc ggagggaagg gggcgcagtc tgcggaattt
    12541 gtggggggaa gatcgaaaat atcaggattg gggggagaag cgcttatttt tgccccaaac
    12601 atcagcgatg agtcgctatt gaaagcagcc accaacccgg cctgttttat gcccgcgtgc
    12661 aggttcgccg ccgatgggcg ccgccaactt ccagaaaaac cttcggatat tttttcataa
    12721 tatcgggacc tgctcggcca ggattctatc tctgaggccg gggtggaggg atctgtaggt
    12781 cagaaggtcc acccgtcgat ttacggcatc ttccagctca tttttgagac gagagaggtc
    12841 cagaaggctc tttctcccct cgaactcgat gaggatgtcg atgtcgctct cctcgtccat
    12901 ctcgtcccgg acgatcgacc cgaagaaggc cgcccgctta acctcgtttt ttcgcaagat
    12961 ctccaatatc gtgtcctgca cggcctgcat ctgatctctc atgaaattca cggtgctgtg
    13021 caagtattca taacttttta ttatgccggg ggtcttccaa gtggttggag gctttcgccg
    13081 tcaacgtctt tgtggccgga ggcggggcgc attccctcga accgcccagc gccgctagtc
    13141 ccagtcgcgc cccacctcca ccgtcagact cccctcggcg tgtcctcttc ctccaggccc
    13201 tctggctttt gtggcggtcc cacgccgacc tcgccgggag cttctcgccg gggattccaa
    13261 tcagaagggg tcaaaggctc ttgaccaccg gcacctggat ctccgtcacc cactcctccg
    13321 gcggcatcgc ctcgccccat ttgacataga cctcccgggc ggcgtccgca ggctgaaagc
    13381 cgtttgagaa gacccagccg tagagctggc tgtaggcgtc ttcgaggttg gtgtagggcc
    13441 cacggacggt gagataggcc acctccgctt ctggcagcac ctccaccgcc gccccgccct
    13501 cggcgaactc ggccgtctcg gtcggaaccg gcaccgccac cttgaaccgc acctcctcgg
    13561 gggggacgga ggcggggtcg tcgaagtaga gggcgaggcc catcggcgag cccgatagag
    13621 cggggatcga ctttttcgcc atccaggccg ccagctcctc gtaggccttc gggattgtta
    13681 cggcgaaggg gaggcccttg gcccagaggc tggccacccg cagggcgggg accttcttga
    13741 tctcgatatc gtatggaggc atattggcac ctttctaaag ctggcatgaa gtcgacctaa
    13801 tgtcttaaaa agatgtcgca gtcataatgt gtggtcttgc agctcatcga attgcgcccc
    13861 ggcggcgcta gtcccctttg gcgcccacat cctctgggcg gtggcccagc tgctcctcca
    13921 ccagaacgcg atcgccggcc ccgccttcct ggtggcgtcc accccctttg cgtcgccctg
    13981 atcccacggg aggaggggat gatggaagac gaatttggcg aagagcgtga gttggtgcgg
    14041 aggcaactcc ccccccgcct ctcaaagttt cagactcaac ccctgggcga tagccacgtc
    14101 agcttctgcg tctcgaccga tagctctgag agtgaatccc ttgttcatcc acgcatctga
    14161 caacagaggg tctaattcaa ctgcctgttc ataagcttct aaagactcgc tgtaattgcc
    14221 agataaatac agcatccaac cctcattact caaagcttcg gcatcagtca atcctagctc
    14281 tctggacttg ttaagggcat ctatcgattc tgaatgacgt tcaagtccct ggagagcaaa
    14341 agacttactg tgccacgccc tcgcattcga ttggttcaat tcgatggatt tattgtagga
    14401 cgcaagagca tcatcatatt cgctcatggc gtagaacgcg tccccttttg ccacccaagg
    14461 gcgttcgtct tcttgatcta gcttgatggc cgcatcgaaa gcattgacag attcgctgtt
    14521 atatcctgca agtaggaacg cgaagccttt gtttctcaac ggcgtcgccc agtcgggatt
    14581 gatcctaatc gcctcatcat aagccttgat ggcctcggta tagttaccta ggtttctgag
    14641 agcatcgcct aagttattcc acggagttgc ccagttggga ttagtcctga tcgcctcgtc
    14701 ataagcctgg atggcct
//
LOCUS       DASM01000011           22615 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_57832, whole genome shotgun sequence.
ACCESSION   DASM01000011 DASM01000000
VERSION     DASM01000011.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 22615)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22615)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22615
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_57832"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         4374 a         6913 c         7379 g         3949 t
ORIGIN      
        1 cgccggtgaa cagtccgtag ccgtacgcga cctgtaccac atcgtcggcg gtcgcaccgg
       61 aacacgcgag cgtgcgggcc attagatcgg cccagttgtt gatgtcgcgc tccgtatagc
      121 cgacaaccgt gatttgacca gtcgtgccgg aggaggcgtg cacccgcacg atgtcgtgct
      181 tatcggcggc gaacatccca tacgggtagt tcgcccgcat gtcgtctttg acggtgaagg
      241 gcacgtcggc gaggtcggca agcgtcgcga cgtgcggtct tacgcccgca tcgtcgaact
      301 tctcgcgata gagcgggacg cggtcgtaga cccgggcaag caacgcgttg agtcgatcga
      361 gctgaacctt ctcgagctgc tcacgcggca tcgccagaag tcagccgcgg cacagctatc
      421 ccgcgccgac gtcgatcgtc gtcgacaagt ccgacttccg gctctacttc ttcaagaacg
      481 acgtatacgt caagagctac ccggtggcca tcggaaggac gaatgcggag acacccgtcg
      541 cgatgtggcg gatcgactcg aagtactacc ccagtcccac gagcgtctac ggccctcgca
      601 agatgcggat gtatcgcaag agtggctcat actacgtcta taccaggtac accatccacg
      661 gcacgaacga tccatcgagc atcggcacga aggcctccgc cggctgcatc cgtctgtaca
      721 acaaggacat cctagatctg ttccctcgcg tgccactgag aacactcgtc cagacacggg
      781 agtagcgccg gtcggggtta tagcctcggg atatgacgag gggccggcga atgccggccc
      841 ctcggatggt tcctcaagtc gagctctctt ctagttgctg gtgcccccgg ccactaccac
      901 cggagacagc tcgggctgca gcgagagtgc gagttcgagc gcgtcctggt ccctgcgggc
      961 cagccagaat agcgtagctg cggtcagcat cgggccgatc aggaagacgg tcatgatgcc
     1021 ccagaccccg gcctcgaact gatagatgac gccccaggcc acaacgccgg ccgatgcgag
     1081 cacgagcaac gcagctacaa cgcgctcgta gtaccagccg atgatcagcg tcgccgcgat
     1141 ggcgacgagc ggaatgaggg ccgctatcaa cgcggactgc aggccgctct cctggaacgc
     1201 atagaggccg gcgaagctcg ccaccgacca gaagagacca cctgcgatga cgaaaccgcg
     1261 ggcaacgcca cgctcggtcg cgatgcgcat ccgggtatcg gccaccttgg ccacggagac
     1321 ctcgcgtgaa gacaccaggt cgtaggccat agcgccgaag agcgcaggta ccgcgatgat
     1381 cgcgagcgct gcgccgacga gtccaacgag tatgagtgcc cccgctagga tgatggacat
     1441 gattctctac ctccaagtga ttcgggccaa gatggcttag tgaacctatg gagagtatgt
     1501 ccgcacaccg cgggcggtgg gttcgggcac gattgctgtc ttgttagggt tgtgtgagcg
     1561 taccgaaggg caccatgccg ccgcctcaca ccacggggag ctcgaagccg aacgtcgtgc
     1621 cctcgccggg agccgagtgg acctcgatct gcgagccgtg ggcgtccacg atctccttcg
     1681 cgatcgcgag gccgagcccg gttccaccct tgccgggtcg agcctcgtcc gcgcggtaga
     1741 agcgctcgaa gacgcgcgag agctgatcgg ctgcgatgcc ggaaccctcg tcggcaacct
     1801 ccagaagcgc gccgttgccg acccgctttg cgcgcaggtg cacggtcgtg ccggccggag
     1861 agtgtttgag cgcattgtcg gtgaagctga ggagcacttg gaccacgcgg tcccggtcgg
     1921 cgaggacctg cacgtcttcg acgggcggtt cgaccgcgag ggtcaccccg gcttgctcgg
     1981 caagcgtatg catcacccgc gccacatcgc cgagcagatc ggtcacccac tgcggctccc
     2041 tagcgagctt caggctcccc gcatcgagct gcgcgagtgt cagcagttcc gtcaccagtc
     2101 ggccgagccg gtcggtctcc acctgcatcg tggcgatgaa gtcgtcgcgg acctcgggct
     2161 catccttcgc cccgtcggtc aacagctcga gcatcccctt gagtgcggcg ataggcgtgc
     2221 gcatctcgtg gctggcgtcg gcgacgaacc ggcgctgtgc ggcctcgatg cggcgctgct
     2281 cggtcacgtc cgcgagcagg agcacagctc cgtcgaccgc cccctcggca tcgaacagcg
     2341 tcgtgcagtt cagcagcacg gtgagctggt cgagctgcac gatgcgggcg atgctctgcc
     2401 ccgcgagccc tgcgcgtacc acgtcgagta cgtcctcggt gctgacaacc gcgtcagcgg
     2461 gcagcccgag tgcgtcacgc tcgccgagat ccagcaggtg cgcggcctcg ggattgatga
     2521 ccctgaccgt gccctccgcg tcgaaggcga ccactccctc ggtcatcgag tcgaccacgg
     2581 ccgcgatctg tcggcggctc tcctcgatgc cgccgaacgc ctcgccgagc ttgaccgcca
     2641 tgcggttgta ggagagtgcg aggtcctgga cctcgtcggg gatcagaccc gtaggcagac
     2701 gctgctcgaa gtcaccctcc gcaatcgccg ccgccgcccc gagcatcccc tcgatgcgcc
     2761 gccgtatcag ttcggagaac ccgaagccga gcaagccggc aaccaccagc gagatccaga
     2821 aggtgaccca gaggcggtcg cgaaccgcat cgaggatgcg cagcgagtcg tccaccgagc
     2881 tcgcggtgac gatgactccc tctctcgcgc ctgatgcatc gaggactgtt cggccggcta
     2941 tgacccagcc gttcggccgc aggtccatgt ccgcgtgggg agcgttctcg gtgagtgcgg
     3001 tctcgcgggc cgttgccagc gcgctctcgg gaggcgccac gtcgtaggag gactcgacga
     3061 gcgtgccgcc gcggtcgtag acccagatgc ccgcgccgta gatgctccgg tactcgacga
     3121 gcaccgagcg catgcgctca cggctctcgg gggtcaccgg aaagtccggc tgaagcctgc
     3181 gtgccagtgc cgaggcgttg cgcacctcgt tggccttgcc catctcggtc agcgtgacct
     3241 ggaggcctgc cgagagcgat ccggacaaga tgagcatggc gaccacgatg acgaccacaa
     3301 agagcgcggt ctgccagaac ctgatcgacg ccttgcgccg gggcatcgcc ctacggctcc
     3361 ctcaggcgat agccgtatcc gcgcacggta aggatgtagg cggggttctt cgggtcttct
     3421 tcgagcttct cgcggatgtt atggatgtgc acgtcgatgg cgcgctcatc gccgacgggc
     3481 gactcgtccc agagagccga caggatcgcc tggcgcgagt aggcggtgcc gggttcccgt
     3541 gtgaggtgga ggaggatccc gtactcggag ctggtgaagt ggatcggcgt tccggcgagc
     3601 ttcacctgac gggcccgcgt gtcgatctcg agatcgccga cgtggatgac ggtcttctcg
     3661 tgagatccct cggtgcgtcg caggatcgct ttgacacggc tgaccagttc tcgcggcgag
     3721 aacggcttcg tgacgtagtc gtcgccgccg gcggtgagtc ccgcgacgcg atcgctgacc
     3781 gcgtccttcg cggtcaggaa cagcacgggc acgtcgttgc ccgactggcg cagccgctgc
     3841 agcaccgcca tgccgtcgag gtcgggcatc atgacatcca gcaccatcgc gtcgggctcg
     3901 aactcgcgtg ccgcctgcag ggcgtggaat cccgatcccg cggtgcggac gtcccatccc
     3961 tccatccgca gtgccatcga cagcaggtcg gtcagcatct gctcgtcatc gacgacgagg
     4021 acgcgaagag cggacccgtc caggcgacgg agtgcgggtg cggacgcggt ggtggtcatg
     4081 tgtccattgt gcgcgcgttc ctatgtgctt cctatggagc aagttatgcg gtggctgtga
     4141 aatccgcgca agcgcggcca cgtcgtgaac gcgcttccgg ttccgccgat gtacaggact
     4201 cgcttcgcat tcatggtgcc atcgtgtccg atgcggcgcc cggcggcccg tagagtgtgc
     4261 gcggaggtca ccatgacagt cgtggacaat gccatctatg tgggcgggca ccgcacttcg
     4321 gacccttcgt cactggatca gaccttcgag gagatgagcg ctcggcacgg catggcctgg
     4381 atcggactgt accggcccga tgagagtgaa cttcgcgaga tcgtcgagga gttcggactg
     4441 cattcgcttg cggtcgagga tgcgctgagc gggcaccagc gccccaagct cgaccattac
     4501 ggcgactcgg tgttcatcgt gctgcgcccg gcgcgctacc tggactctga ggaggaggtt
     4561 gagttcggtg aggtgcacgt cttcgtcgga cccgggttca tcgtgacggt gcgccacgcg
     4621 gagtctcccg atctggcgaa ggtgcgcagc cggctggagg gcgatccgga gctgctcgcg
     4681 aggggtccga tggccgtgct gtacgggatc gtcgacgagg tggtcgacga gtacgggcct
     4741 gtcgtcgcgg ggctcgagaa cgacatcgac gagatcgagg accagctctt cgcgaacgac
     4801 ccggccgtcg cgcgccgcat cttctccctc acccgcgagg tcatcgactt ccaacgggcg
     4861 gtcggcccct tgatcccgat cttggagagg ctccagctcg aggcggagtc cttcggcatc
     4921 gatgtcgagg tcggacgcgg attccgcgat gcgcacgacc acgtcatccg catcgttgag
     4981 cgctcggcga gcttccgcac catcctcgag aactcactgc tgctgcacgc caccctcgtc
     5041 actcagcgcc agaacgacgc gatggccgag atgaccgagt tcagcctcac gcagaacgag
     5101 caggtcaaga aggtgtcggg ctgggcggcg atcctcttcg cgccgacgct cgtgggcacg
     5161 gtctacggca tgaacttcga ctacatgccc gagctccgct ggcggtgggg ctatccggcg
     5221 gtcctcctct tcatgctcat atccggggtg atcatgctcc tctacttcag gaggaggaga
     5281 tggatctgat catccggctc agagggccga tcctcccgga gagatccggg gctcgtctcc
     5341 tcaaaggggt gggatgggtg agacggtgat actccgatgg atagagatgg tcatccccga
     5401 gaaggagagg ccggggctgg agagggtcct gacgaggggc ggccttgaga tcatggggat
     5461 atggatcgag ccggtcctgg ggatttggga gaggccgacg atgggcgtct ggatgaggag
     5521 cttcgacgag aggatgatcc tggtcaggat cctcgtcccg gcggagaact ccgaggcggt
     5581 catcaacatg ctggatgcgc ggttcaggag gaacgatggc tacaggatgg tcgtcctccc
     5641 ggtgacggca tccctccccc ggcccccgat cgacgaggag gagaggagag agcccgcccc
     5701 caaagagaag aacaagaagg gaagccagcg gatcggaagg gaggagctct acgctgaggt
     5761 cgcctcggtg ggggtctccc gggtctacgt cttcatggta atcctatcct ccatcgtcgc
     5821 cgccttcggc gttttgaaga acaacgtcgc cgtgatcatc ggggccatgg tcatcgcccc
     5881 cctcctcctc ccgaacgtat ccttctccct ggcgacgatc ctgggggacc gggggctcgc
     5941 cgtcagggct ctcaaggcga acgtggtggg ggtgggaacc gccatcgcct tctcctacct
     6001 cctcggcacc ttcctccccg tcgacccgga gatcccggag atcgcctcca ggaccggggt
     6061 ggggctcggc gaggtcgccc tagccctcgc ctcggggagc gctggggccc tcgccttaac
     6121 cacgggggtc tccgccaccc tcatcggggt gatggtcgcc gtagccctcc tcccacccct
     6181 cgtcgccttc ggccttctga tgggctcggg gcacgagagc ctcgcctggg gggcgatgct
     6241 cctattcata gcgaacttca tctccgtcaa cctctcgggg gtggccacct tcctggccca
     6301 gggaataagg ccctcgaaat ggtccgaggc gcggagggcg aagagggcct cgatcgtggc
     6361 gatggtcctc tggatcctga tgctagccct ccttatctac ataatcctga tgtaccaggc
     6421 ggggatcgcc atatgaaggg gttcggcgat ggagggaggg cggataggat ggtggcaggg
     6481 gagaggagat cgaggaagcc ctctctagag gcggccatct ccatcttgag cgtcctctct
     6541 cccccggcgg aagcctctcc cgccgacccc gaggcggcgg cgagggccgt caggcgggtg
     6601 accatcactg gcctcgccat caacgtcctc ctctcagccc tgaagctcgc ggcggggatc
     6661 gtcggaaaca gccaggcggt ggtggcggat gcgatccaca gcctcaccga caccgtcacc
     6721 gacgtcgccg tcctggtggg ggtgaggttc tggtcgaggc ccccagacga atgccacccc
     6781 cacggccacc agaggatcga gtttctggtg accatattca taggcctcct cctcgccgcc
     6841 gtcggcctgg ggctcgtcta caacgccctc gccaccctcc acgacctcca ccgcacccct
     6901 ccgggtccta tcgccttctt cgccgcgatc ctctccatct tcacaaaaga gctcctctac
     6961 cgatggacta tctcggtggg gatagagatc aggtccaccg ccctcatcgc caacgcctgg
     7021 caccaccgat ccgacgcctt cagctccgtc cccgccgcca tcgccgtcgc cggcgccgcc
     7081 ctcctcccgg ggctcgccat cctcgaccac ctgggggcga tcgtagtctc cgtcttcatc
     7141 ctccaggccg cctggaggat cgtccggccc ccggtggagc atctcatcga ccagggtgcc
     7201 cccgaggaga tccttctggg gataaggcag ctggcctccg ccacccccgg agtcgaggag
     7261 gtccacgcca tcaggacccg gcacatcggc tccgggatcg aggtagacct ccacgtcctg
     7321 gtagacccga ggctcaccgt cgaggagggg cacgagatct ccgaggaggt gaagcggagg
     7381 ctcatcgagt acgtctccga cgtggtcgac gtcgtcgtcc atctggagcc ctacggccat
     7441 gagaaggagg ggcgaggccg ggtatgagac cccgccccct gcccggcggc gcggcggcgg
     7501 ggtggggccg ccggagggac tgagagctag acgggaccag aaggccctgg tggagaaaat
     7561 ggagatgaat accggtttta aggatgaata aaacttttaa gtcgtatttt ggaagaatct
     7621 gaacgttctt atatagtttg aacgatatct gaacggttat agtccggatt ggttcggctc
     7681 taattataac catcgttcag gcctcaggga ggttgtcgag gagggcggcg ccggtgcttc
     7741 ggcagagccg tcctccacta gtgaggtatg aatgatgaga acaactggac ttttgataac
     7801 cctggcggtg gccaccctca tcgcggtccc cgccttctcg atgccctggg agaagggtgc
     7861 cgggcggcac ggcgggttcg acgggatgaa cctgaccgaa gaggagatgg agaatatgac
     7921 cctgggagag ctgagggccc tgaaacagga gacgaatctg gcccagtgtc cccgagaggg
     7981 cgagaccggc gactccccca tgtaccagcg gagcaggagc ccgggcccaa tggaccgggg
     8041 cgccatgacc caggggcccg gcgccagggg accgatggac cggtgcttcg gatgtccgtc
     8101 tattctgatg atggacctga ccgatgagga gatcaagaat atgaccctcg ccgagctctc
     8161 ggatctgagg gcggagaaga tgaccgagct cgggaacatg acctccgagg agatcgaggc
     8221 cctctggcag gagaagaggg gcgagatgga gaatatgacc ctcgcggagg tgagggagag
     8281 ccggcaagac cgtcccttcc tcggctttgg agcctgcctc ctcgccaccg atatcaccct
     8341 ggaagagctg gaggggatga ccctcgctga gatcgaggct ctccgggagg agaagatggc
     8401 tgagcttgag aagatgacga tcggcgagct cgaggagctg aacgaggcga ggagggcgga
     8461 gatggagaat atgaccctcg ccgagctttc ggagcagagg gaggtctgca gcatcctggg
     8521 cctcggcccc gacgggatga gcagaggtca gggctgccag ggcgagcggt tcgggatgag
     8581 atctcccggc atgaggcagg gataccccga gtgccagttc ggaaccagag cctcggggat
     8641 ggaagatcag ggtaggggag tctttggagg ccggatgaag aaggccggat tcccgggtga
     8701 ggagtagccc gggagattct gggggaaagt tccaagatat ccatatcgga atcgggttgg
     8761 ggggccttgg gcgctgaggc ccagggcccg accacccaac cccgaattcc aggcctgagg
     8821 tgacctccga tgatgaaaga gacgacattt actgattcct ttatcgctcc atccaccatc
     8881 ccgatgaaga ccgccttggt caggagcggc gtcgccttgg gcccggtggg ctcgcccttc
     8941 caggcacccc tccccatcag gatggaggtc gaggggctcc ggctcctcag cccctcccag
     9001 atccgagagc tcctatcgag cagaaggagc atcgatgagg tcaagatccc gatcgaggag
     9061 gaggcgaacc cctccttcgt atactgggga gatctgcagc tggggatgga gaagttccag
     9121 ctctcggatc tctccttccg gactctggac tccgagacgg tcctcagagc cgacctcgcc
     9181 gaggtccggg gggtaggcac ctggtccccg gcggcgaaag gggcggtagc gggaaggata
     9241 gagacgagag agaccgaggt gggcggatgg aaaggcgtcg ggagcctcct gatcagagag
     9301 gggcccaaga gaggtaggta ccacctccga ctggaggccc cccccgagga gggatcccct
     9361 tgaagacctc cataatcccg gtcgtcctgg cgatggtctt ctcagtcgtc tccaccgccc
     9421 tctcataccc cgccgaagat gtatcgatcc cggcggaccg gccgaacccc ggacctgtgc
     9481 cgccggaggt gggaggggac cgccaccgcc ccggcgccga cggcgagggc cgccccccgg
     9541 ccggcccctt ccgggcgggg ggggtagccc tggagagggg cgagacctac ttcctccata
     9601 tatggctgat cgataccaaa cggatccctc cggccctggc aagggagatg ctgaaggaga
     9661 ataggagcct cgacgagatc cgagaggaga tggcggggtc tggaggcttc aacgccgccc
     9721 tgggagggat gaccctgggc gatcgccact tcgtcctggt gaggatcaac cagaccctcg
     9781 acgggaactg caccatcctc gacgccgacc tcgtcgattt cagagagggg cagagagcgg
     9841 gcgaggacag gacggcgggg cacatcaccg tcaggacctg cgatgtggac ggggtccagg
     9901 tggggtgcgg aaacctgacc ctccacgccg gaggggcgac gtcctacagc ctgaccttcc
     9961 cctccgacca gatgggtccg gaaagaaacg gcgcccccga gacggccgga ggggaaccat
    10021 gcccctggta gaggtgaagg gcctggagaa gaggtacagg gtcggagaga tggaggttcc
    10081 ggtcctccgg gggatcgacc ttgagatcga ggagggggat tttgtggccc tgatgggccc
    10141 ctcaggctgc ggaaagagca ccttcatgaa catcctcgga tgcctcgacc gcccctcgaa
    10201 tgggacggtc ctcctgcagg gccgggacct gggaaggcta tccgaccggg atctggccag
    10261 gataagaaga gacgagatcg ggttcgtctt ccagagcttc cacctcatcg gcaggatctc
    10321 gaatctgatg aacgtcgagc tccccatgac cctcaaagag ctgcctaagg aggaggggag
    10381 agatcgagca aaagatctcc tggaggcggt ggggctcgga ggccgcctcg gcttcagccc
    10441 caccaacctc tccggcggcg aaaggcagag ggtggcgatc gccagggctc tagccaacga
    10501 tccctccttg atcctcgccg atgaaccgac gggaaacctc catcagaaga gcagaaggga
    10561 gatcatcgag atcatgaagg ggctcaatag agaagggcgg acgattatca tggtaaccca
    10621 cgacgcagaa acgacggagt actacagccg cttcatctgg atgagagacg gccgtgttca
    10681 ggagggcgca tcttgaatcc gaatcatggt cctgcgccca actgggctat ggtcctggcg
    10741 gctgctaccc tgatcctggc tctggcccta gcccccggga ccctcgccaa cacctacggc
    10801 tatatctacc gccccccgga gccgacgata gtgatcgagt cctcggtatc gccggaggtt
    10861 ctaaccgttc cctagatcgt tggggcgatc ggaatatcca acgtcatgat gctgacggtg
    10921 aaggagagga cctccgagat aggggtgatg aaggcgatcg gcgctaccgc ctgggacgtc
    10981 cgggcccact gcctcctgga cgcggggatg ctcggccttg cgagcagcat cgtgggaatc
    11041 atcctcgggg ccctcgtctc gaagctggtg ggtggggtgg cgatgatacc cacggaggtg
    11101 acggtccagt cggtggctct cggtctcctc ttcggggtgc tgatcaccac caccgccggg
    11161 atatatccag cgaacagggc cgcgatcctc gaccccatcg aggccctccg ggggagggat
    11221 ggtcaaaact cgcctcttcg ccggcgggat ggtcggcgag gccctccccg agagccctct
    11281 ggcgaaaaat gctggcgcag tttacctcct ctccctcgcc agggggccgg gaacgagggt
    11341 cctagagctg accgctgacg aatcgaggta gtatgtatga tgatgacgaa atttagaaac
    11401 cttggtctgg actttctgat ctgcacagcc ctgatggcag cagtttcagc cgaggagatc
    11461 tccctgggcc cggtcacggt gaacctcgac cttgaggttc ttgagggcta tacgatagac
    11521 gtgggtgacg ccatcgaggc ctctcactgc aagcgtccag agttcgatta tatgatatat
    11581 ccagtgacaa tcgaaagcga tgacagccaa atagagctgg aagtccacga gctggacgag
    11641 ccgatggaga tgacgccttg ggtcttagag cactccctga taaccgcggg tcttccatca
    11701 ttgacgccaa agctggaagg tgcgggggcg agcttcaccg tggacgggca cgacgccatc
    11761 ttggtgacgg ccaaggatgg agataagacg gtttacggtg ccatctacac cccggatgag
    11821 gtggacggct ggggaaggca gctcttcctc atatgctcag acctcccttg ggatgtcacc
    11881 gagatagtct ttgagtccat agaggtggac gcctaaaaca tgcccaaatc ggccagaatt
    11941 tcgtcgaggt ctgaacagat cgatggagat gaattccatg aagctcaacg gcttgttgca
    12001 cagggttaac aggatctctg cctgggtcct cttcgtcctg atgattgcgt acatcgtcac
    12061 aggatacgcc tggagcgagg ggataatatt gagccgaaac gaggcgatct tctggcatac
    12121 gagcctggat ctccctctcg tcttcttctt cctcgcccat gtcctcatcg gcgccaagtt
    12181 tgccctgaga aggtacgggg tcgaccaccc cctagtcaac ccggcgctcc tggtcatcgg
    12241 ccttgccgga ttcgcatcgg cgttggtgat ctcgtgaact actccgcagc gccaggatct
    12301 cccaacggat ccgacggcgt gcaccgtccc gaacccgccc cgagagccct cggcgatccg
    12361 ccctcccgac tcagcagatc cctccagagt tcgccggggg ccgggcacgc gggtccggta
    12421 aggcggcggt ccggagctgc attcagctta tattcggatc agatctatat ctcaagaccg
    12481 caggaaggca tcagatcgga ccccccctcc gggagcccgt cttcgatagg aggctactac
    12541 aatgatccgc aacaagatgt ccaagtctct gatggccatg gcgatcatct ccatcctcgc
    12601 agccaccgcc ccgatttcgg cggcggagga ggtcggagag gacctgacca acctgaacct
    12661 ggtcgatgaa caaccggtcg ccggcagcga gatggggacg aacgccaccc cgaacgagac
    12721 cacgaacgta accaacacca ccggattctc caacttcacc aatgagtcaa tagccgttga
    12781 cgccaacgag accgcgatcg gctccctcgg caacggatcc gaagggaccg aggtggaggc
    12841 gataggggtc ggtcccgggg cgctggagcc gacggaactg aggtatctgg gtggcggcct
    12901 ggtggcatct gccgccggga acgctacggg gctcagcacc atagattctg aggcgacgcc
    12961 catcagcacg accagtgcaa cggagccgat ggaggcagcg accctgggtg ggggcctgaa
    13021 cgccactggc gaggtggccg ccgacctcag caccgtcggc ttgatctcag ctccggccag
    13081 cacctccacc gcctcggagc cgatggacgt gagggtcctg gggaacgtcc tgggggcgcc
    13141 gacgggggag gacgtgaggc ccctcagcac cctcgggaac gttacggcaa cggcagccaa
    13201 cgcgacggag gcgaccttcg cctccctctc caacgcttcg gctgaagggg ggaaggcgac
    13261 caccgacgtc agggccctta tggaggcgct gggaatcgag ctctgaggtc tcgaaatcgc
    13321 cctgagagct atagtccccg aaagccgggg gggctaaagc cccccctgct ccgggcggca
    13381 tcactttcga aatttaacaa tgatccaact ggatcgtgcc gcctactcag ccggaatact
    13441 gataccgaaa gttataattc tattaacttc ttaagatggt cttatggcga gaggagacgg
    13501 actgcttctt gaagttgtcg actataaaga cgctcaacgt tggcgctggg tgctgaagga
    13561 cgcctcagga aacttcctta tagaccagga ggtgaacctc gaccttcaag atcctaacac
    13621 cacggccatc ttggacctcc agagccatct ggaccgccac tcctctcctg acgatcggat
    13681 ggctgaacag aagcggctta ttgacgagtt gagccgctgg atcggctcca aggtcttcgg
    13741 cgaggtggca gagagtctct ccaagttacg aacccccgtc actgtccggg tcgtcttccc
    13801 gccggaggcg agggcagtag cccacctacc cctggagcta gctgaagttg gaggtaagcc
    13861 cgcggcgctt cgagacctca gcctcgtctt cgaggtgaag ggctcagagc ctgtagagca
    13921 cgagggggtg ggggagaggc tccggatgct ggcgatctac agcctcccca ccgatgtctc
    13981 ggccctagcc ctccggaggg aacgctacca gcagatgcgg ctgatccgaa gcatcgccca
    14041 gacccacaac ctgtgggtgg agctgagggt cctccagtac ggcgtcaccc gcgataacct
    14101 tcaagaagcg ctggaggagg gggagggttg ggacctgatc cacatctccg gccacgggag
    14161 gcagggggct atcgtcctgg agaacccgga cgggacgatg gacgaggtca gctccgagga
    14221 tctggcggac ctgcttagcc tcgcccgggg caggctgaag ttcgtgaccc tctcgtcctg
    14281 cctctccgcc gccgccacgc tggaggagac cctgaactgg ctgaagatac cggttccaga
    14341 ggaggtaaaa aaggctactt cctgctttga ggcggacggc ggccccatgc cagccctcgc
    14401 tcaagaattg gtggaccggc tggactgcgc cgtcctagcc atgcggtatc ccgtcggcga
    14461 cgacttcgcc atcaagctcg cctccgagct ttacgagcgg ctttttggca aggggcagcc
    14521 gcttccaagg gcgctgcagc tctcgctgag ggaggctctg gaggaggagc tgaggtgggg
    14581 cttcaacgcc ggatcgccgc cccttagcct ggcgacccct gctctattcg gcagggaggc
    14641 ggcgaagctt cagataaagc cgcccaagat cccccgatcg gagttcacga tcccctctcc
    14701 cggtctcgcc tacttccccg acgagcctga gcggttcgtc gggcggagcg gccccatgat
    14761 cagggcgagc tcggccctgg cggtcgagag cgataagagg ggggtcctct tccacgggat
    14821 ggccggtgct ggaaagaccg cttgcgccct ggagctggcc tatcaccaca gccgaagccc
    14881 caggttccag gggttcgtct ggtacaaggc ccccgacctg ggcaaggaga tcggcggggc
    14941 gctcctggat ctggcgacgg agatggagcg gcagcttccc ggcttcaaga tggtccacgc
    15001 tgtcggggat gccgactcct tccgagactt cctgccgcag ctgagagggg tccttgagaa
    15061 taacagcatc ctcgtcgtcc tggacaacct ggagtcgctt ctgacgagcg acgggagttg
    15121 gcgggacgag cggtggggga tcttggctaa aaccctcctc gatcaagacg gattttcccg
    15181 gacgattttc acgtcccggc ggctccccaa agacctcgac cgcgacctgg tggccgtcga
    15241 gccgatattc gccctctccc tgaacgagtc gatcgtcctc gcccgggaga tggagaacct
    15301 ggggaggctt ctctcgggta agagcgtcgt cagcctcgag gaggggcgca gcctcgtcag
    15361 caggacgctg gccctcgtcc agggccaccc caagctgatc gagctcgcgg atgcccaggc
    15421 aaaggatccc gccgccctgg agaggcacct ggggagggcg gcggcggcct ggggggatga
    15481 gggccgcctc tccagcttct tcgaggaggg cgagtctatg gaggacgccg agaagttcct
    15541 ggaggttctt aaagggtgga ctcaggggct atcggagacg ctctcccccc aagccaggac
    15601 cttattccac ctcctctctg ccctggagga gcccgaccgg gatagcagaa tcgtggagat
    15661 ggtctggccg aacctttgga ctgccctcgg gcgatccggc tcgcctccgg ggatcgacga
    15721 tctcctgggg gagctgaaag ccctcgtgga ggtccagagg ctgggggagg ccgtcaggta
    15781 ctccctccat cccggcgtcg ccgaggcggg cctcacggag ctggaagtgg ggttccggaa
    15841 ggctgtggac tcggagatgg ccggtctctg gggcgccgtc ttcggcgccg ccagcgaggg
    15901 cgagacggaa ggcgcgggtg ggtgggtcgt ccaggcgggg ctgaggtcgg cgccttacct
    15961 gatgaggcta ggaaggtggg atgaggcctc cagcctcctg gagcaggcga tatatcgtga
    16021 ttcgtcttcg gggacggcgg ccgcggtgct tccgatgctc cgccggatcg ccgaagctac
    16081 tgagggaacc gatcgagagc cgatcgacac cgcagtccta gccaaggcgt tgcttgcggc
    16141 gggccggtgg caggaggcgg aggcgatgtt gaggtctctg ctgatcaaat gctcagacga
    16201 agggaagctc tgggaggttt cagggttctg tacttatctt ttccggatcc ttctaatgac
    16261 cggcaggttt gaggaggctc tccatctcgc cgaggagatg aagggttatt cccgcaaggc
    16321 gggtcttgga ccttggaccg agctgtcgga cgaggcatca aggctccagg cgctgaataa
    16381 gctcggccga tacgacgagg tcttgacggc cgtggaggag ctgaggggga agatggaggt
    16441 cctttccgag aaaagagctg agaatgaggc agttgagccc tggaacgtca aagaggccat
    16501 cctcgatgct ggccgagcgg cggctcgtga ctctcgagag taccggctgg ccctggagct
    16561 taacgcagaa caactgaagt caaaagaagg tcgcggagcg acggatctga atctggcggg
    16621 ggccgccttc accgattact ttcctctcct ccggctgaaa cgttacaaag aggccggaag
    16681 actgctctgg aattgcaagg aggtctttga gagggaaaag gatatcgaaa tgataggaaa
    16741 ggtcttctca gctctcgccg acctgatgga cgagatgggc cagaccgacc aggcggccag
    16801 gtttgaggag gacgctcttc ggtacgaata tctgaccggc gataccgaat ccgtatcagt
    16861 gagccacaac aatctgggca attaccacag aaaaatggga tcatctaaat ctcttgccca
    16921 tcacctggcc tcagccgtca tccgtttgct cacccgctcc gggatgctgc cccaaaccct
    16981 tcaaaacctc tccatctctc tcggcacgtt cggcgccgag gatctgccag ccagcttcga
    17041 tcggctctgc gagagggtgg aggaggtcga gggggtcagg ttcagggggc tcttcctccg
    17101 gatggcgggg ccggacggcg acggcgatga ggtgatgcag gcgatcatcg agaaggcgag
    17161 aggtgaaaat tcgtgaatga ggattggaag aagggctata acgccgaaag tgaaaagcgt
    17221 cagtacgccg acgtctatgg gactccagat atttatgggg ccgcaggtgg caaggccaat
    17281 agctatcgat gctcccactg cgacaaaata atccggacca gggatataga gtggcggggg
    17341 acgacggccc actgccctaa atgcgggaca gagcttgagg ttcagggcag atcggacggg
    17401 accagatctt ctcaggggtg aacgatgctc ggaaacttcc tggataaggc atcaggcctt
    17461 cttgaccgaa acttcctctt agcctactgg tttcctgcct tcatctccgc ctcgatagcc
    17521 gtcctgatag gagcctggcc ccggggctgg ggctccgctc tggagctctg gcagctggag
    17581 gggatggtca aagggcagga gggcgggctc tcggctcaga tcctgatcct cgtcggagcc
    17641 ctgatcctga tcacagtcct ggcctatctc ctcatgcccc tcaccaggcc aacggtccgc
    17701 ttctacgagg gctactggcc ccggtggctg taccgccggt tcggcgacat gccgaggtgg
    17761 ggcgagaggg ccatctatga gaggatgacc aaggatcttg accaggccga agtgaaggag
    17821 gaccggccga ggttcaacga gcttcaggcg gtcctcttct acggctatcc taagagagag
    17881 cggctgatgc ccacccgcct cggcaacgcc ctccgagccg ctgaagacta ctccaagggc
    17941 gcctacggcg acggcatgga taccgtcttc tggtggccgc ggctctggcc cctcctcccg
    18001 gagaaggtcc aagaagaggt cgaggaggct ctcaccccca tggtcgcctt gctcaatttc
    18061 tcgtccctgg tcgccatcgt ctcaattgga ggctccctat atctgggcgg gctggggttt
    18121 ctgaaagaag ccgttctggt ctttgcggta ggggtggtcc tggcctttat atcgtatcgg
    18181 gcggccgtca gccaggccca gagctatggg gagaggatcc ggtcggcggt ggacctttac
    18241 cggttcgatc tcttgaaatc cctccaccag actctccccg agacgctgga cgatgagatc
    18301 gccctctggg tgaagctcat gctctggctc tacaacggcg atcggggttg tgttgcgaag
    18361 atgaagtact atcacgattc ggggagcgga tctggtggcg aggggacgcc ctgatccgac
    18421 ctagatccgt gatgagatac tttgaacttt tttgctgacg tggctgtaac tgcctgttct
    18481 tccgatgagc tagaaaaccc ttttatagaa ggtcaaatag tctgctcccg agaaggaggc
    18541 attatatggc aggattaggt ggacagccga tattgattct cagagaaggc gctcggcgag
    18601 agcggggaag agaggcgatg gccaacaaca tcatggcggc gagggccgtg gccaaggcgg
    18661 tcaggtccac cctcggcccc cgggggatgg acaagctcct cgtagacacc atcggagacg
    18721 tgacgatcac caacgacggg gtcacgatcc tccgggagat ggaggtggag caccccgccg
    18781 ccaagatgct ggtggaggcg gcaaaggccc agaacgacgc cgtcggcgac gggactacga
    18841 cggtggcgat cctcaccggc gagctcctta agagggcgga ggagctggtc gatctcggcg
    18901 tccatccgac gatgatcgtc caggggtaca gcatggccgc cgataaggcg atcgaggtcc
    18961 tggaggggat ggcgaggaag gtcaccgaga aggagaggaa cctcctggag aagatcgccc
    19021 tcaccgccat gaccgggaag ctcgcaggaa ccccggactt ccagatctcc aaggacgccg
    19081 tggacatggt cctggcggtg accgaggtga cggaggaggg gaagaaggtc gtctccctca
    19141 agaacgtcat cgtcgagaag aaggccggag agagcatgga cgactcccag ctcttccacg
    19201 ggatcatcat cgacaaggag aaggttcacc ccaacatgcc caagaaggtc gagaaggcga
    19261 ggatcgcaat ccttggaacc cccatcgagg cccgggacac tgagacgaag gcggagatct
    19321 ccatcacctc ctccgaccag ttcaagatct tcgcaaacca ggagaaggag gcgatcaaga
    19381 aggtggtgga gaaggtgata gccaccggcg ccaacgtggt cttctgccag aaggggatcg
    19441 acgacctcgc ccagagcatc ctcgcaaagg cggggatcat ggccttcagg aggatcagaa
    19501 agaccgacct gaagaagctc gcccgggcct ccggggggaa catcgtcacc aacctcgacg
    19561 agatgacccc cgcagacttg ggccgggcgg agctggtgga ggagaagaag gtgggcggag
    19621 cccccatggc cttcgtcacc ggccttgcta gagaggggac ggtctccctc ctcctccggg
    19681 gcggaaccca gcaggtcgtc gacagtctgg agagggccct ggacgacgcc ctccacgccg
    19741 tcgccgccgc cgtcgaggac gggaccctcg tggcaggagg cggcgccccc gaggtggagc
    19801 tctacctcaa gctccgagag tactcctcga acttcaaggg gagagagcag ctcgccgtcg
    19861 ccaagttcgc cgatgcggtg gccgagatcc ccaaggccct ggccgagaac gccggctttg
    19921 acgccataac caagctgacg gagatgaaga gcagccacga ggcggggatg aagaccggcg
    19981 gcctcaacac ctccgacggg aaggtcgtgg acatgtggga gatgggggtc gtcgagcccc
    20041 tccgggtgaa gacccaggcg atcaagtccg ccaccgacgc cgcaaacttg atcctcagga
    20101 tcgacgacgt catcgcctcc aagaggacgg agcagccccc cgccggtcca ggtgccggga
    20161 tgggaggcat gggcgggatg cccggcggca tggggggcat gggaatgggc atgcccccgg
    20221 ggatgatgtg aaggggacga ccgcctccgt cgtcgccccc cccggaaggg ggtcagagcg
    20281 gcgccgaatc gatcggtgga cctcccctct cgaggcggag gagggatcag aaggctgcgg
    20341 ataaaacagg agggtgaacg cgcctccaac cccctccgcc tcccccacca aaaagagatc
    20401 cgatggacgg gatccgaacc tggcgcctcc tctctcccgt cccgggacct atttttcagc
    20461 ttttaataag atcctagaaa tgccggccat tttgatgagg ccgaggaaga aggatggggg
    20521 tcggatccgg aagggctcac ttgaacccca ggatcctctc ggaggagtag ggcttcctgc
    20581 ctctcatgat cccgtaaccc aggagcatgg caaggccgga gcagaggacc acgatcccca
    20641 agagcaggag ctcgaaggtg aggaaggcct cgatccccgc atgcctcgcc aggtttggag
    20701 cgatgatgat ggcagccccc gtcagggaga ggaagaggcc ggaggagagg cgcctctcga
    20761 gcctctcatc cagagactcc tctccacccc gatccaccac cccccgctcc aggaggtaca
    20821 tcttcttctt gtgatcgaga taataaacga aggccaggaa gggtatccct tccatgatca
    20881 gcaggaggac gggccaatct gtcacggttc atcaccttca ccgggcttta tctaaattcc
    20941 catagttaat cctttcgcac tgcagtttta atccgtaata acgatatatc cagaggatat
    21001 ggagttcgat ttattggcgc gccacctttg aaaggatgag atgtatcctc cgagctgaag
    21061 ccacacctcc ccatcttcgg ggcggcagat atgaagcccg cccgtcggct caaatccggg
    21121 ctgccatccg gatccatttg ggagatagag gtcccgctgc gctccatcct tctcgccacc
    21181 ccaaagacgg ccttttgcga gccgataaat tacgccccat acataatatt taaagatgat
    21241 gtttccataa aagctttgaa gatcggtcgt cgaccggctc gaagagggtc cggagatggg
    21301 atgggacgtc ccatataatg ccccaggaga ttgatttaga aggtggacca aaatcgatgg
    21361 caattgcctc ctctgaccga agggggcggc ccaaggaggg gccgacggcg aggaatcgaa
    21421 gaagcctctc acgggatctg tcaggaaggt ttatttagga acagatcctc taataaggga
    21481 ttaggtctga agcaattccc tttcgaccag ccgccgtcgt aagccgctca tttttcccgg
    21541 atcgacggcg aaagaggaga taaaggtgat gccgttcgtg gaagcttcaa atctcgaggc
    21601 cgggatcgcc gaagggcggt ggaggaagct tcgagatcac atcatattgg tcacagccgc
    21661 cgccatcctt ctccttctgg cgatcctctg catccccgaa tgcccacccc cctgatgatc
    21721 ccgcctccgg ctccgggccg gctcagggga ggtccctgac catgtatggg ccccggagct
    21781 ggtagcctcg cctccggtag taaggcctcg ccccgatacc gctcgttacc cggagggatt
    21841 cataaccccg gtcggaggag atctcctccg cctcggcgag gagggtcgat cctatgcccc
    21901 ggtgctgcca gccacccctc ttccccaggg gggtcatggg gccgtagacg tggagctccc
    21961 tgacgtgtgc cccctccgag agccggaggc ggaggaagcc cgccagggtg ccatcctccg
    22021 cctcaaagga gaggaaccgc tcttcgccgc cgcaagcccc gtaggagagg tctctgaggg
    22081 atacctccgc ctcctcagag accccccgga gccccgcctc ccggcagcgg atgcaccggc
    22141 aggacccccc cctagatagg agccgcgcct tcgccagctg gcggaggttg ctctttttga
    22201 ccccggcgga tatcgcgggg gcggggatgt ccctctggac ccgctggagc ctggtgtagg
    22261 gggggatcag ctcctttatc ctcgatatca gctctgccgc ctcgccgtcc cccaggggct
    22321 cgtactcgcc ccggatgtag aggtcgtaga gctccgtccc ctccaccacc agggcgggat
    22381 agatcttgag gtagtcgggc cggaactcct cgccatcgaa tagctctcga aaggaggcga
    22441 ggtcccgctg ggggtcagat ccggggaggc caggcatcat gtgaaacccc accttcagcc
    22501 ccgcctcccg gagggcccgg ttcgcctcga tcgtctcccg gacggtgtgc ccccgtctca
    22561 tcctcaagag aagttcgtcg tcggtggact ggacccccag ctccaccttc gtccc
//
LOCUS       DASM01000012            2629 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_265855, whole genome shotgun sequence.
ACCESSION   DASM01000012 DASM01000000
VERSION     DASM01000012.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_265855"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          487 a          885 c          850 g          407 t
ORIGIN      
        1 tccagaccaa caacgtcgac aactgcgccc gggtctgcca cgccccttca gtaaccggcc
       61 ttgccgcctg cttcggcagc ggcgccgcca ccaactcctt cgaccagatc gaagacgccg
      121 acgtcctctt cgtcatcggc tccaacacca ccgaggccca tcccatcgtc gggctgaagg
      181 tgatcaaggc tttcaataac ggagcaaaga tcatcgtcgc cgacccccgc aaaatcgagc
      241 tcgtcgatag ggcgaaggtc tggctcaacc tgcggcctgg gaccaacatc gccctcctca
      301 acgggatgat gaacgtcatc cttcgggagg ggctggagga tggggagttc atcgccagcc
      361 ggaccgaggg gttcgaggag ttcaggaagg tggtcgatgg atataccccc gagagggccg
      421 ccgagataac cggtgttcca gcagccgata tcgtcgaggc cgcccgcctc tacgccagcg
      481 ccgagaaggc gatgatcatc tacagcctgg ggatgaccga gcacgtcacc ggaacctcca
      541 acgtcatctc cctgggaaac ctcgccatgc tgacggggaa cgtcggccgc aggtctacgg
      601 gggtgatgcc gctgcggggt cagaacaacg tccagggggc ctgcgacatg ggcgccctcc
      661 ccaacaacca cgtcggctat cagccggtga cggacccgaa ggttcggtcc aagttcgagg
      721 aggcctgggg ggtccggctc ccaccagatc ccggcctcac ctctacagag atgctggagc
      781 ggctggcgga ggggaggggg atccgggggc tctacatcct gggggaggac cccgcccaga
      841 ccgaccccga taccaaccgc gtccgctcct ctctggaggc cctggagttc ctcgtcgtcc
      901 aggagatatt tcccaccgag acgaccaagt acgctgacgt catccttccg gccgcaagct
      961 tcgccgagtg cgacggtacc ttcaccaacg gggagcggag gatccagcgg gtgaaccggg
     1021 cgctcccccc tctcggtggc cttgctaact gggagacgat ctgcctcatc gcccagaaga
     1081 tgggctatcc catgagctac gccgacccct ccgagatcat ggatgagata tcatctctgg
     1141 cgccgatctt cgccggagtg agcttctccc ggctgggtgg cctgggcctc cagtggccct
     1201 gcccatcgat agaccacccg ggtacggaga cgatgcacct cggctgcttc agccgggggc
     1261 tggggaagtt caaccccgtc gagcacaggc ctcccgccga ggagaccaac cccgaatatc
     1321 cgctgatcct caccaccggc cgaagaaggg agcactacaa ctgcggctcc atgacccgcc
     1381 gctcaaaagg gataatgtgg gtctggccgg aggagacgat cgagatcagc cccgccgacg
     1441 ccgaggatat gggaatcgag gacggcgaga cggtcctcgt caggtccagg cggggcgagg
     1501 tgagggtgaa ggcgagggtc accgagaagt cgtcccgcgg cgtggccttc atgtccttcc
     1561 actaccggga cgtgctcacc aaccttctca ccaacgccgc cctggacccg gtggcgaaga
     1621 cgccggagta caaagcctgc gccatacggg tggagaaggt cccttcgtga agagaggaag
     1681 ggaccccctc cttcccgggt ccccgtcagg ggaccaatca tatttaaaaa agcagcatct
     1741 tactctaccc catgagactc ctcctcttcg acccatcctc gggcgcctcc ggcgacatga
     1801 tcatggcctc cctcctggac ctgggggccg accctgatgc ggtaagaaaa gccgtcgagt
     1861 cggtcggctg cacccttgag atctccaggg cggagaagca ccacatctcc gccgcgaggg
     1921 tgaggatcca tgccggcggg aagaggtaca ggaccctggc ggaggcgaga tcggtcctgg
     1981 cggcggcagc cctcgccccc caggcccgcg accggtcgat gaagatcatg gagatcctgg
     2041 ccgaggcgga ggggagggtc cacggcgtct ccggagagga ggtgcggttc cacgagatcg
     2101 gggccctgga tgccctcgcg gacatcgccg gaagcgccgc tgcctgggag agcctgaagg
     2161 ccgatcgggt cgtcgtcctc ccgatatccg tcggctcggg aaccgtcatc gccgcccacg
     2221 gccgcctccc ggtcccagcg ccggcgaccc tggaggtcct ctccgcctcc ggcctcctct
     2281 ggaggggcgg gccggtcgaa ggggagctct tgacccccac gggggcggcg atcctggcgg
     2341 cgttcgccga cgaggccgcc gccgaatatc ctctgattcg ggccggggcc tcgggctacg
     2401 gtgccggatc gaaggatttg gcggtcccaa acctcctgcg gaccgtcatc ggtgagccgg
     2461 cggcgccgaa gcgccctggc gagagccatc cccatccccc ccacgaccgg gtcgtccagc
     2521 tggagacgaa cgtcgacgac gtcaccgggg aggtcctggg cggcctttta gagatcctga
     2581 tggaggcggg ggccctggac gtcaccatcg tcccggctct gatgaagaa
//
LOCUS       DASM01000013            8083 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_133083, whole genome shotgun sequence.
ACCESSION   DASM01000013 DASM01000000
VERSION     DASM01000013.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_133083"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1441 a         2654 c         2673 g         1313 t
ORIGIN      
        1 ccgccgagga cctctacgag gcgatagccg ggaccgcccc ccgatcacgg ttcgggatcg
       61 ccttctccga ggcgagcggc ccctgcctcg tccggtgcga ggggaacgat ccggagctga
      121 tcgatgcggc gaaggaggcg gccctcgccc tcggctgcgg ccacaccttc gtcgtcctga
      181 tccgggaagc cttcccgatc aacgtcctca acgccatcaa ggcggtccag gaggtctgcg
      241 ggatcttctg cgccaccgcc aaccccctgg aggtggtggt ggcggagacc gagcagggga
      301 gggggatcat gggggtcatc gacggctcct ccccaaaggg ggcggagacg gcatccgacg
      361 tctccgatcg gagggggatg ctgagaaagt tcggatataa gctatgacca ggatggatcg
      421 ggtcgtcagg aacgggcggg tctacgtcga cggccgcctc cagaacgtcg acctctggat
      481 ccggggcggg aaggttgccg acctcggggg aaaccatcag gcagaggaga cgatcgacgg
      541 ccgggggann tccacgtcca cttccgggac cccggctaca cctacaagga ggactgggag
      601 acgggatcga cctcggccgt cgccggcggg gtgacgaccg tcgtcgacca gccgaacacc
      661 gaccccccca ccctggacga gaggtcctac aggatgaagc tggagacggc cgagaggcgt
      721 tcggtcgtcg acttctgcct caacggcggc ccgggccgga tgaagaggct cgcggagctc
      781 ggggcctccg ccatcggaga gatcttcacc tacgaacaca gcgacgacga cctccgccgg
      841 atcctggagg agacggcgag gtcccgcctc ctacccacga tccacgccga ggatgggaag
      901 gtgatccggg agaatactgc gcccctgaag gaccggaggg agccggagct ctactccatg
      961 gccaggccga atctcgcgga ggcgaaggcg atcgagatgg tgctgaagat gaccgagcgg
     1021 gcccacatct gccacttaag cacctccgag gggctggagc tccttcgggc cgccaagagg
     1081 ggaggaaaga gggtcagctg cgaggtggcc ccccaccacc tcctctttac gaagagggac
     1141 tggcagaggc aggggaccct cctcaagatg aacccgcccc tccgggactt ccgggacaaa
     1201 gacgccctct gggaggggct tcggaagggc gacgtcgacg ccatcgcctc cgaccacgcc
     1261 ccccacctcc ccgaggagaa gagggacgag atctgggacg ccccgccggg ggtcccgggg
     1321 gtcgagacga tgatgcccct cctcctcatc gcggttcgat ccaacctcct ctccctcggc
     1381 aggatggtcg acgccctctc ccggaggccg gccgagatcc tggggctctc gaccaagggc
     1441 gggatcgccc gggggaagga cgccgacctc gccatcatcg acccccgggc ggcgtccgag
     1501 atccgggccg accggctcca cagcggggcc gagtggaccc cttaccaggg gaggcgggcc
     1561 atcttcccga agatcaccat gatccggggg gaggtggtct actcggacgg cgagctcttc
     1621 gcaaagcccg gattcgggcg gaacctgccg gggcccggtc ggaggcggag ggtcacggtc
     1681 gggcctcagg aagatgacga ggttgagatg gaataacagg atatttcgag gtgttaagga
     1741 tgagagagga tgcggaaagg atggtgggca gagcggtgat ggagtcgatg aagaggtcgg
     1801 tccaggcgag ggaggtcttc cggaagatgc tgggggacgt tgccgagaac ctgccggagg
     1861 ccgggggggg gcctgccgtg gacgtcgtcg actcccccga agaggtcgtc ttgtacatcg
     1921 acctcccagg gacgaagaag gagctgatag acctgggggt gacggaggac gccgtcaccg
     1981 tcagggcgag gtttgagccg cctcctggaa actacctcca gagggagagg ccggaagcgg
     2041 agatgggccg caggatcaag ctcgccgccg aggtgaagcc ggagctggtg agggcgaggt
     2101 acgaggatgg cgttttagtc gtccggctgc cgaagctgat cgtggtcaag ccccactcag
     2161 tccccatcga ttgagccccg aggatagaaa aaatagaggc tatggcgagc tcaacccttc
     2221 ctcgccaaga cctcttttgt tacgatgacg taggtgtact cgatcgatgc ctcctggcgg
     2281 ggcgttaggg aaagctccca ctccagcccc gtcgccgtcg tctccttcgg ctccggctcg
     2341 gcggatacga cctcggcccg gaggggctta gtatccgaga cctccacggt ggcgttccga
     2401 tcgaggttgc tcttgatgtt gagcaggtat gtccaggtct ccgtcgtcgt cttcatcgcc
     2461 gaggtggcgt tatcggctcc ggggacctga gagatctcct cctcggtggt gtagctcgcc
     2521 atcttccggg agaccttcag gtccgccgag gagccgacga cgatctccgc ctccgtcccc
     2581 acgggggtga aggggatctc cacccccgag acgtactcgc cgttcctata gaggagggca
     2641 gagccggcgg gccaggggtc cttcatggtg ttgttcgctc tcacgacctc ggtcaccggc
     2701 ccctcgctat tccggtatgc gtcccagagg tagctcctca ggatcggggc cgtctcctct
     2761 aggaggggaa atcccacctc tttgcccatc ccgagatcct tcctccccgc cagctcgtag
     2821 atgtacaggg tctccagctc ccccgtggcg ggggctggag ctgcggcggg ggcataatcc
     2881 atcgcctcct cttcagcgac ggcgcgcatg gcaaagcccc ctacggcgag gtacttctcc
     2941 aggggcgcga cctgaggcgg tcccgagacg agcttcagct tgaccccctc caggtcctcg
     3001 ccgaagttgt ttctgacgat cgcctgggcc gagacgatca ccgactcctc ctccaggtgg
     3061 acggagtaga tgggcctcca ttggcctgcc atcgtcatca ggtaggccag atcaaacctc
     3121 tggaccccgg tggaagagac gttcagggtg tactccaggt tgtcgccgcg gacgaccttc
     3181 tcgacggtgg cgttcgtcgc cccaaacctc accgaatcca tcttcaccga ggacgggagg
     3241 tccaggacga gccggtggac cccctccgtc acctccatct ccccggtcct cctcaccgtg
     3301 gcgatgccgt cgggatagat cgtcaccgag tcgacgggaa ggctgaggat cgtgatcgcc
     3361 tcgacccccg caggcccgtc gtcggcgagg gcaaccgctg ggagggccag gatcaggacc
     3421 agaaaagcca gaatcttctt catgcactcc gctccagatt tctatctaag tcataggctc
     3481 ccttattata ttatgctttt ccagcgccct ccgccccgac ggagggggat ggaccggtcg
     3541 gcaccaaatc cccgtcccgg gggtgcgtcg caacgcttat atcagtatat cgaacttccc
     3601 cccctgaatc gtacggtgat gctctcatgt ttctggactt ctggagagat gtcgaggcgc
     3661 tcctccgaga cgccttagat cggtgcgggt ttcctctgcc ccaggccgac gggaaggtcg
     3721 aaggtaacga gcttctgctg gagatgagcc cccacgccga cctcgcctcc accatatcct
     3781 tccggctcgc acccatcctc aagaagagcc cggccgagat ctccaggaag atccaagagg
     3841 cgatcgtcct cgaggggaag gggaccgtcg accgggccga ggcgatgggc ccctacctca
     3901 acttcttcgc cagccgcagg ttcctcgact cgacgatccg ggaggcggta gccgacgact
     3961 gctggaccgg gtcgatgaac gagagggtga tcgtcgagca cacctccgcc aaccccaacg
     4021 gccccctcca cgtcggccac ataagaaact ccgtcatcgg cgacaccctg gtcaggatcc
     4081 tcctccgggc cggctgccag gtcgaggccc agtactacgt caacgacatg gggaggcaga
     4141 tcgccatcgt cgtctggggc tgcggcagat accccctgga cggctcgaag cccgaccacg
     4201 ccatcgcaaa agtctacatc caggcgaaca aagacctcgc cgcagcgccg gagctgaagg
     4261 agggggtcga ccggctgatg cagctctacg agggcggaga cccggagacg gcggcgaagt
     4321 tccggaaggc ggtggagtac gccctctcgg ggatcgacga gaccctgagg aggctgaaca
     4381 tcactcacga ctcccgccgg tgggagagcg agttcgtccg ggacgggtcg acggcggggg
     4441 tcttaaaggc cctggaggcg acgggcctga ccgaatggga ggaggggtcc ctccagctcg
     4501 acctctccgg cgatggcttt gagaagaagc tcgtattgaa gaggtcggac gggacctccc
     4561 tctacaccac cagggacctg gcgtaccacc ggtggaaggc cgaaagctcc gaccggatga
     4621 tcgacgtcct gggggctgac cacaagctca tctccggcca gctccgggcc gccctccgcc
     4681 tcatgggggt ccgggagccg gagatcgtca tcttcgagtt cgtctccctc cccacgggat
     4741 cgatgagcac gaggaggggg aagttcatct ccaccgacga gctcctcgac gaggtggagg
     4801 cccaagccta ccgggaggtc acggcccgaa ggcccgagga gggggaggag ttccggcggg
     4861 ccgtcgcgag agaggtggcc ctgggggcgg tgaggtacga cgtcgtccgg gtatcgccgg
     4921 agaaggcgac ggtcttcgac tggaaggcgg ccctggactt cgagaagctc tcggccccct
     4981 tcatccagta ctcccacgcc agggcctgca gcatcctcga taaggccgga gccgtcagcg
     5041 gcttcgaccc ccgcctcctc tccagccccg aggaggtggt gctcgtcaag aagatatccc
     5101 agttcgacct cgccatcgac ctctccgccc gggagctgaa gccccacctc ctcgccacct
     5161 acgcccggga gctggcggag atcttcaacc agttctaccg ggcaagcccc gtcctggagg
     5221 cggatcccgg agtccgggag gcgaggctcg ccctcgtgga ggcggctcga aactgcctcc
     5281 ggtcgaccct ggataccctg gggatcccgg ccctcgaatc gatgtaaatt ggaggaggcc
     5341 ttccccgagg gcaagagggg cctggaggag aggggatccc cgctccgccc cccaccggag
     5401 agggaggaag gggccttcgg agggtgatgg cgcctcccgc cccggccggc cctctcccca
     5461 gaaagcctcc aaaacttttt gtactatcag ggggataaag agggagaggg ggtgttcctc
     5521 ttgagcttga aatcgggatt gattttgtta ttgataatct tctccgtagg ggtggcctct
     5581 gctcaatgga ccgaagagcc gatggccgat ctcgtcggag aagggacgga cgaaagcctc
     5641 atgatggcgg gctacagcgg cggcgaggac gggccgtccc cagagctgat gaggatggtc
     5701 ggaggctccg accccttcgg cgccaccggg ccttcggggg ttggcgaggc ctattacagc
     5761 atggaggctc cgcccggcag cgggcctgaa gttcagcagt actcgatatc ggggcacgag
     5821 ccctcgacgg tctacttcgg aggagaggcg acctcctacg gggcctacag caccacctac
     5881 ggcggcacaa actccctctg gatcctgggg tcctggagct ggtcgcagta cgcctacgct
     5941 cccctggggg cctacctcca gcttctggcc tactcctcca gcggcgggtc agcggacttc
     6001 tacgagatct accccagcgg caacgtagtc tacaagcgct actccttctg gcccggctac
     6061 tccaggatca acttccacgc cgacgcgacc ggccgccaca tcctcctatt catcgcaaac
     6121 aaccagccct ccaacgccat catcatcgac gtggggagcg ggggatggcc gccgacccca
     6181 ggcccgggac ccggccctgt gccctccttc gcccgggtta cggtcagatc cagctggctc
     6241 acgggttaca ccgtcaccgt cgacgacatc cagacctaca gcgacgccag cgacgggagg
     6301 cgcgacggga tcatcagctt caccgtcccc ggaaaccagt accacagcgt caagataacg
     6361 agccccggat atttgaggtc ttactaccgg ttcttcagga gcggctacgc ctacaccctc
     6421 accgtatgag gggatggggc ggcggaccgg gacgcggaaa agatgagcgg tccggggaaa
     6481 cgtcccccgg accagtccct attttgcaga cagctggagc cgatccccac cccgtcttca
     6541 ccggagggga ttgggctgag gggggatgag gggaggcaga agcggcgata tccgagagat
     6601 ccccctcaga tcctcgccgc cgcctcgatc ggccgcgcct ctccgatcgc cgccttcatc
     6661 ccgcggatca gctcctccag ccccgcaagg tcgcccttgg agatcaggtc gacgcaggcg
     6721 gatccgacga tggcggcgtc ggctccggcc cggatcacct ccgccgcctg ctccggggtt
     6781 gagattccga agccgacggc cctgggcagc tcggagttca cccgctcgat gagggccttc
     6841 gtcgactgca gcacctcgga cctggccccc gtcacccccg cccgggcgac gaggtagagg
     6901 aagccgctgg aattttctgc gatgaggggg atcctctccg ccggggtatt cggggcgatg
     6961 agggatatca ggtcgaggtc cgccctcctg caggccgccc gcaagggacc cgcctcctcc
     7021 ggcggaaggt cggggacgat gaggccggtg atgccgctcg ccccgcagtc gccagcgaac
     7081 ttctcaagcc cccgctggta gatcatgttg tagtagccca tgaagaccag ggggatcttc
     7141 gccctgatcc gggaggccat ctcgaagtag acgtcggtgt tcatccccgc ctcaatcgcc
     7201 ctctgggccg ccgcctggat gacggggccg tcggctatcg ggtcagagtg ggggagcccc
     7261 agctcgacga cgtcggcccc ggcccgggcg aggagctccg ccacctccac cgtcgcctcg
     7321 gggctggggt cgccggcgca gatgtaggcg atgaggaggg ggcggcgcct gaaggcctcg
     7381 gcgagcctca tctccccacc ctcccctcat gctccatcac cgtcgccagg tccttatctc
     7441 cccttcctga gaggttgatg atcgagacct ccccaagggc gtcggggttt cttagggcgt
     7501 agccgacggc gtgagaggac tcgagcgctg gaattatccc ctccaggacc gagagccgcc
     7561 tcaggccgag gagggcggtc tcgtcgtcgg ccaccgccgg cgtcaccctg ccgacctcgg
     7621 cgaggtgggc gagctcgggg ccgacgccgg ggtagtcgag gcctgcggcc agggaatagg
     7681 actcaaggat ctggccgtag gggtcctgga ggatcttggt catcgccccg tggaggaccc
     7741 cctcgtcccc gaagttgagg caggcgccgt ggtctgctat ctttcggccg gaggtgatcc
     7801 ccctcccccc cgcctcgacg gcgatcagcc tcgtctcatc cttaagaaag ccggagaaga
     7861 tccccatggc gttggagcct ccgccgacgc aggcggcgac ggcatcgggg agcctcccct
     7921 cggcggcgag gatctgctcc ctcgcctcgg tcccgatgac ggactgaagc tccctcacca
     7981 tcagggggta ggggtggggc ccggcgaccg tccccatcag gtagtgggtc gtctcgtagc
     8041 tcccagccca gtccctcatc gcctcgttga tggcgtcctt gag
//
LOCUS       DASM01000014           14239 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_3020, whole genome shotgun sequence.
ACCESSION   DASM01000014 DASM01000000
VERSION     DASM01000014.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 14239)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14239)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14239
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_3020"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2738 a         4401 c         4384 g         2716 t
ORIGIN      
        1 ttgtgccgtt caaaggaggt gaagaggacg ggaccttcaa cgccgccggt cagggagtac
       61 cagcgtccga actccgctgc cgttgcggtc ttgccgttcc cggcgtaggc gtggaggagg
      121 actatattct gggtgtcgaa ggcccggtcc agggcgagaa gcgtctcgtc tctgccaaag
      181 aatccgacgt cgggcctctt cggaaggccg ggatcgatga cccctctttt cgtatcgggc
      241 atcgagtctg cacttcggat cttgatcgca aacttctctt cagatttcgg tttggggaag
      301 agggagactg gcgcagcggc atagacgata ggaaccggcc agtcctgtag aggccggggc
      361 tcgtagccga tctctcgcag aggctggtcg tgcagaacct tccggccgag ggtggctgcc
      421 tcgtccaggg tctgaccttg ggtgagggcg gcgtagaggt ctgcgacgaa ctgggctgct
      481 gtcaccacgt agacgttata gcgcatggcc acgactccaa cgacgcccgc gtccatcacc
      541 tcctgggcca gggacccgaa ggctctcacc cgcgaatgct ggtcagcctc tattgctccc
      601 gccgcagcat ccggctcgtt gggcgattcg gcgtgggctg aacggcaggc gttcaggacc
      661 aggaccggaa cttcggtctc caccagcagc tttcccagca acgatccgcc caccatctga
      721 acattctctt ttatggcagg attttcgaag aggagatagc cgtggggccc aggccgactc
      781 gatcccagga ccaacctgct caggcccctc acgagaccgc caaatccgcc tgaatcttcc
      841 atctcggcgt aggttccgtg accgtcgaag tggacgacat gatagggttc gcccttatcc
      901 tttgcggctc gcagcgtccg gccgagctgc tcgaaggtgg gcggccggag gacgtcgagc
      961 tggaagtgct ctcgggcttc atcgctcagg cctttgacga tccggcttgc taccgacctg
     1021 aagggaacgt catctctggc tccaggacgg cagatcacaa gcaagatgcg aatcggcccg
     1081 gtggcggctc cgggaagctt cggcctctga gccgcctggc tgtaagaccg gacgaaggat
     1141 ttcgcccgga gcgctaaagg cacgtcggtc tttaggtccc ggatcagctc ccagggtatg
     1201 gctgtggccc cctcgacatc ggtcgcgatc tccacgcgag tgtcgttcag ctgagatctt
     1261 aaaacagccc aaatatcgcg ggcgtcgtcg ttcgcctgaa atacggcctt gaaaaggtca
     1321 gttcccagtt ctgccatgcg attctcaatc cgggcggcta tcttgggagc tggatcttgt
     1381 gggtactgga ggtagtcttc gagataccat cgcaaatctt ctcggtcctg gtcagatagg
     1441 acaaaagcaa attctgattc ggcggtctgg cgggctagac cgtccccctc cagagctatc
     1501 tcgacgcgat atctgtcctc gccttcggag atctgggtaa agcggagagt ggtcatggga
     1561 gattcctcgt agattctttc tcaaagtcat gacgcgccaa tccatcaaaa atcttgcgtt
     1621 ctggtctcct ctccgggatc tggacctaat aactcaatag cgtccccacg ccctctgagg
     1681 attcaagaaa agcttcattc agagagataa cggatttcaa cactggataa tcgtgctatg
     1741 ccgccattgc agtcgagggg agggattctt ggctggcgct tcaaaacaaa gatcagctct
     1801 caacctccaa aagcaaggtc tcgatctcgg ctttgagcgg cctgatctcc tcgttcaaga
     1861 cgttggtgac cacgtcccaa tctatcccga agtactggtg gatgagcttg tccctcatcc
     1921 cggcgatgag cctccagtcg atctccgggt gaccatcctt catatcttgg gatacgttct
     1981 tgacagcctc gccgatgatc tccaggcttc gcacgcaagc cctgctgaga actttatcac
     2041 tcataagatc ctcggcgtcc acgccctcac attccgatag aagaaaatcg atctcgtcga
     2101 ggatgtgcct caataaggcg gcgtccttat tcagaccata ccacctccct ctcaacgtaa
     2161 ggccttatgt aggggctcag ccccttgacg gtcaccaggt ccacctctct atcgaagagc
     2221 tcttcgagga agaagactag gcccatgaag tttctgaagg tcggctcctc gaactccacc
     2281 aggacgtcta cgtcgctcct ctccttctcc tcccctcggg cataggagcc gaagatgccg
     2341 atcttgcgga cgccgaactt ccttttaacc tctttctcgt tagccttcag aattctgaga
     2401 gcgtccatgg caagcccccg ggcgctgctc ctcgtcggca ggccgaactt tgcttaaatt
     2461 ggatcttcta ccgtaaatag gtaccattaa tctatgccca atacctcaat agcgacctca
     2521 acccctccga cgacgcgagg gcccgcgtcc tcccggacga actccacctc cgctcccatc
     2581 tcggttggca tagccggata gggctcccta aaagatctgg gctcacccta tcaccctacc
     2641 atccgcgcca gctcctctcc gatatacggg tccatctccc cgcagcggcc gtgggtggcg
     2701 caatcgacga ggtgcatcgc ttttggctcc agagccttct gataggtccg gctcgccgaa
     2761 tcgacgggaa ttactgtatc gttcttcgag tggatcatga cgaaccttcg gggcgggatt
     2821 ctctcgagat aggtatcggg atcgatggat cgcaggaacc tgaccgcgtc cgggccgctc
     2881 atcctcaaga gatcgatctc ctccccctct acatcgtaac cgcaggtgct gatggcgacc
     2941 acgccccggg acctcctgtc cagggcggag gcgatgatgg cgaaccggcc gccgttgctc
     3001 tcgccgaggt atacgatccg gccggggtcg atctcgggct gcatcctcag gacctcggct
     3061 gccgccaggg cgtcgtggac catcttgtgc tccgtcggct cctggccgtt gatgaacatc
     3121 tccagatcgc cccccacgtc gacgccgccg cggttccgct gatcgagggc gatcgtcgcg
     3181 tatcccaggc cggagaggag ctctgcgagg ccctgctcct gctctttggt gacggtcgcc
     3241 ccggggagga ggacaacgcc tggaaagcct tctggtctct tggccgacgg ctctcggagg
     3301 agggccgcca cctccgcccc ccggctgctg aagcggagct cccggaggct tgaggctcca
     3361 gcctcctcgg atgagatcaa acgatattcc ggggcaggta tccggtaatc cagcaatccc
     3421 tcttcagaaa ccctccactc aacgcccgcc tccgaggaga ggaagacccc tatgaagagg
     3481 gcgacgatcc cgagggcagc gatcgaggtt gctattgaac gtctcttccc gatattttcc
     3541 tccccctcag cccttttctg caggaatggt acttgatcgt tacggctaat cgcagcccgg
     3601 aagggtggag tgagcccctt atttttgacg gtatccgccg gcgatctgac tgatattctg
     3661 cccctaatac ctcagaagcg cccccacccc ctctcccgag gcgaggcagg catcctcctc
     3721 gacgaactcc acctcggccc ccgtcctctc cgccagctcg tagagctcct cgatgacgtc
     3781 gacggccgaa gtcggcccac cgcagctcgg acaggccggg ggcggccgcc tcctcgcctc
     3841 cagggcctgg cacctctcgc agatccagcc cggcgccgag gcgtccttga ggaggacgag
     3901 gacgctcacc cggcccgcct ccagggcggc cttcgtcgcc tcaatccccc ggaccgcgag
     3961 gccgcccctc aggatctcgg cccggagctc ctccgccctc tccatcgaca gcaccctctc
     4021 gtcggcgagg gcgatctcgt ttccgatctc gacgagctcc ctttggggcg cgtccatggg
     4081 gaggtcgacg acggcgagaa ccttccgtcc cagatcatgg gggaggagct ccagaaactc
     4141 ctttttggcg tcgccgggac cggcgacgac gagcccccgc gtcttgtgcc ggaggcagga
     4201 ctcccagacg tcctcggcca cctccgagag gaaggatcga accgccccct tccggaggcg
     4261 gttgaacctc atctggctcc aacccccctt cttgtgcttt cccatcaggt ctgccgatag
     4321 ccgtcccacc tcctccggga cgtcggagcg gacgcagaag agccgggcct tccgggagtc
     4381 gaggaggagg aggccgtagt cgaggtagtc gtcccggagc ttcgcgagag ggaggaggaa
     4441 gggggaggtg tccaggacca tggcacgtcc gggatcgatc ccgatcctgt aggcccggag
     4501 gaggggggcg ggggccgagg cgaagacgat ccgccccctt tcgccgggta tgggcctgga
     4561 aaagaggagg tcttcgacgg tggacatcgt ctctctgaag gacgccttca gatccgccgg
     4621 gagggcctcc tccatcgccc tcgacctcct ggcgacgaag ctgcggttct cggcctcctc
     4681 ctccctcgag gcggtgggga ggtagaccga taggaagacg tccttctcgt ctcggacctc
     4741 tgccagccct cgaatatcta tctccgtcac cggggtgagg cgaccttccc cgaccccttc
     4801 gaactccgat ccgggatcca tgcccccggg atggccgatg ggggggaaaa gggtttctgg
     4861 ggaagaggcc cgcaggcctc cgacggccgg ggagaggcgt cacattcctg gcgtcggagg
     4921 aagctccctc ttgtgccttc cggcggcgat ccgcccttcg acgagggcca gcttctccct
     4981 cccaaactcc gccgcgaggt cgtttcggct ccttctttcc tccagccccc ggaggatggc
     5041 gttggcgtcc tggtagctga tccccagctc cccctcgtcg gtctggccgg gccagaggcc
     5101 ggcggtcggc gccttatcga ggatccgatc cggaaccccg aggcgcctcg cgagatccct
     5161 cacctcgtcc ttgtagaggc tggctatcgg caggatgtcg gcggcggaat cgccgaactt
     5221 cgtcgagtag ccgagcagga tctcggtctt gttcccggtc cccaggacga gcctgcccag
     5281 aaggtttgcg tgccagtaga ggatcgacat cctcaccctc gccttcaggt ttccgttcac
     5341 caccgctgga gccgcctttg cggggaggac cgcaaagtag ctcctcagca cctgggagat
     5401 gtcgatctcc tctgcctcga tcccgaggat cgaagcgacc ttcctagcgt cctctacatc
     5461 ctcctttggc gtcacccccg cctccggaag gaggaggcag aggacccggt ccttccccag
     5521 ggcccggacg gcgaggaagg cgacggtcgc cgagtcgagg ccgccgctga tcccgaggac
     5581 ggcacctttc gccccggccg cctccacctg atctcttatg aacttcgcaa gatgggtctc
     5641 cgcatcttct ttggccattg ggggaccgtt atggtactct caaataaagt cctttttctg
     5701 cacaagcttt atgttcgaga agctggtggt ggagagcagc aatttgacga ggaataagtt
     5761 ttatgcgact ttcgatggac ttggaactgc aagatgtgcc gggacagctg atgcagtccc
     5821 tcgagccatt caagaagtac aaggggaaca tcatcagcgt ggtccatcac cgggatcgga
     5881 agacgccgag gggcaccgtc ccggtccaga tcgtctttga gatcgatcca tcaaatatcg
     5941 ataacatcaa aagacacctc caggataatg ggatcgtggt ggtgagggcg ggggaggacc
     6001 ggttcatcga ggagatctcg gtccttctca tcggccacgt cgtccacacc gacctggggg
     6061 acaccataga ccggatcgac tccaccggct tcgccgaggt ggaggacctc tccctgagga
     6121 tgccggggat cgaggagccc tcctccgcct acctgaggat cagggcgacg gggaaagagg
     6181 agatcagaaa gtccctctcg atcctccgcg aggtgggggc ggagaaggac cttctggtga
     6241 tagaacctat cgaggctgag aagatatgag agaggtgagg atttctctgg tggggtttgg
     6301 gatcgtcggc cacggcgttc tggaggtggt gaggaggagg agggagcttt tgaggagcat
     6361 ggggctggac cttcgggtcg tctcggtcac cgacatcaag gggaccctct tcgacggcga
     6421 cgggatcgac cccaccaggc tccacggcgc caaaaccctg gaggggatcg caaacctcgg
     6481 gatcatcggg aaggaggcga tccaggagat cgactccgac ctggtggtgg aggtgacccc
     6541 caccaacatc gtccacggcc agcccggcct cggccacatc gaggaggccc tgaagctcgg
     6601 aaagcacgtc gtcacctcga acaaggggcc tctggtcgtc ggcttcgacc atctgaggga
     6661 cctggcccgg gagaacggcg ttatgctcaa gtacgaggcg accgtcggcg ggacgatgcc
     6721 cttaatcagc ctcatagaga agaacctggt cggaaatgag atcctcagca tccgggggat
     6781 cttcaacggc acctgcaact acattctgac gaggatgatg gaggagaagt acacctacag
     6841 ctacgccctc accgaggccc aggagctggg gatcgccgag gccgaccccg cctacgacgt
     6901 cgacgggata gataccgccg ggaaggtcgt catcctcgca aactccatct tcgggatggg
     6961 agcgaagtac gaggacgtct ccgtcaccgg gatcaggggg ataacccccg aggccctcaa
     7021 cctcgcctgg gagaggggct acctcgtcaa gctgataggg gaggtcccgg ccctgacggt
     7081 gaagccgatg ctggtccccc ggaggagccc cctcgccgtc ggcgggaccc tcaacgtcgc
     7141 ctccatcgag accgacctct ccgggacgat caccgtcacc ggcctcggcg cgggatcgat
     7201 cgagaccgcc agcgccatcc tctccgacat cgtcagcatc tacgcctcca ggcggataga
     7261 cggctgaaga cccggaatca tgaccagctt cgacagcttc gcagagctct gcgcgaggat
     7321 cgaggagatc ggcggctcca tcgagaaggc cgacctcctc gcctccttcc tccgggatct
     7381 ggaggaggag gagctctcgg tcgtcccctc ttttgtgatg gggtccccct tcccggcgag
     7441 ctccgacctt ctcctggggg tggggccgag caccctctac gacgccctct ccaaggcctt
     7501 cggggcggcc cccgccgagg tccaggagct cctccggagg acgggagacg tgggggcggt
     7561 ggcggcggag ctggcgaaga aggggaggtc ctccaccctc tcgaccttca tcgaggggga
     7621 gaggctcacc atcctcgagg tccattcccg gttcctcctc atcgcccggg catcggggcg
     7681 gaggagccag accgccaaga tgaagcacct ccgatacctc ttcagcgagg ccaggccgac
     7741 ggaggcgagg tacatagcga ggctcgccct ggaggatatg aggatcgggg tcggcgaggg
     7801 gatcgtccgg gacgccgtcg ccaaggcctt tggatcgtcg ccggaggagg tggagcgggg
     7861 gtacaacttc accagcgacc tcggcctggt agcccggcag gcgaaggagg ggcggctccc
     7921 ggagctgacg atcgagatcg gtcgacccat aaaaatgatg ctcgcccagc tcgggtccgg
     7981 gatcaccggg tcggtggcgg agatggggac cgcggccgtc gaggtgaagt acgacgggtc
     8041 ccgggtccag atccataaga ggggagagga ggtggagatc ttctcccgtc ggctggagag
     8101 ggtgacccgc tccctccccg agatcgtatc cttcgtcaaa gagggggtcc gggcggaggc
     8161 cgccatcctc gacggggagg cggtggcccg gggggaggac ggaaggcctc tgcccttcca
     8221 ggagatcctg aagcggttcc ggaggaagta cagggtcgag ggggtggcga agaggatacc
     8281 cctggagctg atgctcttcg atctcatcta tctcaacggc cgggtcctca tcgacgagcc
     8341 cttccgggag aggagaaggc ttctggagtc ggtggcggac gaaaagatcc tcgcagacca
     8401 gaaggtgacc gacgatcccg aggaggtggc cgggatctac gaggaggcga tcgcagaagg
     8461 ccacgagggc gtcatgctga agagccccga gtccccctac gcccccggta agagggggaa
     8521 gaactggctg aagataaagc cggtgatgga gaccctcgac ctcgtcgtca tcggagcccg
     8581 gtggggggag gggaggaggg cgaccttctt cggctcctac cggctagcat ccccggaccc
     8641 ggagaccggc cgcctcgagg acgtggggtg ggtggcgacg gggatcaacg acgagatgct
     8701 cgccgagctg acggagctct tccgcgacct cgtcctcgtc gacggagagg ggatggaggt
     8761 ggaggtgaag cccgaggtgg tcttcgaggt ggccttcgag gagatccaga agagcaagag
     8821 ctactcctcc ggctacgccc tccggttccc caggctcgtc cgggtcaggg acgacaaggc
     8881 cgtggaggag gcggacaccc tcgaccgggt ggccgacctt tacgccaccc agcggggtag
     8941 gaacggcaga tcgtcgggct gatttagagg acagattagg gagttgtcga cgttgttatt
     9001 caagaagatg agcgaactgg tcgatgagga ggcgaaaggc cttctatcga ggggcgtgga
     9061 cgtccagtcg gtgatgcccg cggtggtgga gatagtgaac gaggtccagg agcgggggga
     9121 cagggccctc gccgacctga ccgagaggtt cgacggcgtc cggataggga aggtccgggt
     9181 cggcaaagac gagatagaga aagccgggga ccaggtgccg gataagctga agaaggccct
     9241 ggagatcgcc ctggagaaca tcgccgcctt ccaccaccag gagctcgacc gggacctctg
     9301 gatgaccgag acctcctcgg gggttctggc cggccagatg gtggtccccc tcgactccgt
     9361 cggggcctac gtccccggcg gccgggccgc ctaccccagc tccgccctga tgaccgtcgt
     9421 cccggcgaag gtcgccggcg tcgagagggt catcgtctgc accccgccgg ggaaggaggg
     9481 gaaggtgaac cccatcactc tcgtcgcagc cgagatggcg ggagccgacg agatctaccg
     9541 ggtcggaggg gtccaggcgg tggcggcgat ggccttcggg acggagtcga tccggccggt
     9601 ccagaagatc gtggggccgg ggaacatcta cgtcaccgcc gccaagctcc tcctccggtc
     9661 caccgtcgag atcgacatgc ccgcgggccc cagtgaggtt ctgatcctcg ccgactacac
     9721 cgccgagccc gagatcctgg catcggacat gatcgcccag gcggagcacg acccccagtc
     9781 gatatcggtt ctggtcaccg acgacgagga cctggccatc gccaccaggg ccgaggtgga
     9841 gaagcaggca aaagaggcgg cccggcggga gatcgtcaaa gagagcctgg agaggggcgc
     9901 gatcctcgtg gtggggtcga tggaggaggc ggcggccttt gcaaacctct tcgcccccga
     9961 gcacctggag atcgtcgtcc gcgacccgat ggacgtcctc gggaagatca ggaacgcggg
    10021 aagcgtattc ctcgggccgt acacgccggt cgccgccgga gactacgcca gcggcaccaa
    10081 ccacgtcctc ccgacctcgg ggtacgcgag ggccttctcc ggcctcaacg tcgacgcctt
    10141 cgtaaagaag atcaccgtcc agaggatctc gaaggagggg ctcagtctcc tgaaggacgc
    10201 gatcaccatc ctcgccgagg ctgaggggct cgacgcccac gccgagtcgg tgagaaagag
    10261 gctcaaaaaa gagaagcagg agcaggtctg aggctttcag acacaaaata ttaagagcaa
    10321 aaattgctgc gcaccccgca gccgaacttg cggggtgtgc tttctcgcca gcctaaggct
    10381 ctgagacaac gagattgctc tcccgtttgt cccattggga tacgggctcc gcagaatagc
    10441 catccgtcgc cagaccccgg agatgaagct caaagttgta agccaccgcc gggcaccccg
    10501 atagagccgg cggcaaagga tctggttcga attccaacag ctcgtctttc ttgcccaccc
    10561 acccggaggc ggtgttagga cggctctccc caatgaacgt gccggcagat agaccgtctt
    10621 tgcccctggt cccgctcagc cagtacttct tgacgtgtcc ttcgggatca tacgctgtta
    10681 ccctgaactg tatcttgcgg tcagatccca gctggaccac tccgcagttc gtcaccgctc
    10741 ccgttgtggt gccgatgacc tccagcttga tctcgggcag gctgttgtcc acccggatga
    10801 tcggcatctg gaaggcgccg acctcggtac cggatgcgtc atagcggacg atgcggagag
    10861 catagtatcc gtcggccacg ttggcgctgt tccaagtgac tttcagagcg tgctcgtgcc
    10921 agtcagcctc agcctcggta tcgatgttct tataccgccc tgtcgctggg tcgggaccga
    10981 ggaccataaa gtcccatctg tgtaaagcat cgttccactt gcggttgtgg agcggatctg
    11041 tcagatcctg ccagctccca aggacggtat cctcatccgc ggtagctatc tgcaccttat
    11101 atgtggcgac cggcggagcc tcggcgaaaa gcccgaagat gcggagggtg ccgcagaaag
    11161 gctggtggga gacggcgatg ggatcgcccg cctgggaggt ggcatagccc agctgaatcc
    11221 gggttgcgtc gatcgggaga agccctaggc tgatgaacct gaaaccctca tccggcttca
    11281 cacccccggg tgggacgggt atgacatact ccttaggcac gaagtagtcc cggtggaagt
    11341 cttcgacgat ctcccagtcg acgggcccct catagacctc cgtccaagaa ccgtccacaa
    11401 actgcgagat acgggcccgt atgtcgggga ccctgtcggt gaagagccag gtccagaggc
    11461 cgcttgtggt gtagctgaag ttgaaggaga agtcgtagct cccgtcaggc gcggtatagg
    11521 catagccgag gtatccttct gtcaggggcg gctcggtgcc agcgatccag aaaaggggcg
    11581 tgtcaacttc atagaactcg attttggcag ccggcagcgg ctccaccgag atgggagagc
    11641 cgccccaaaa agtagtggcc caaacgaccc cgtgcatgtg gtatgtcttg gtcactttcc
    11701 cccagagcct cacataccct ggaggcagat ggaggtctgt agaagccctg taaatctgtc
    11761 cgtctctatt atagcgagca aagctgaccg ttttgctgag ggctaggctc ttggatgacc
    11821 gaagaggtat tcgcgcaggc aaaatgcgca actctgcggt cggggcttcg tctccatatt
    11881 cgaaggtgac cttatatctt ccgttcttgt caacctcggc cctgaccacg tctatgccat
    11941 ctattatggc atgaacggcc agcttttcac tggcgaaatc gatcttttcc gccgttttta
    12001 cctgtccttc gatcagatag gtggtggaag tctctttcct cccttccagg gcctttacaa
    12061 tttggctaaa gcgcgcctct ccgacctggg gcgtcgccgc aacctggtcg aggctctgga
    12121 atcctccgag ccgcgcccgc ctgtctagga ggctctcggc gatcttaacc ccgatatcac
    12181 gctctccctt tatctcaatc gcactggcta tctcctctgc ggaccgtgca gtattgagaa
    12241 aagaaaggac tttcttcgaa gtattttctg atattcttct ggggtccgcc tttgctgatc
    12301 gtttttcctg ggccattttt tacctccaaa tgaattgaac gaatctatct atcggcttca
    12361 attcttgtat caactaatct atcataaaaa gttactatta ataaactttt cgtatcgatt
    12421 ccaataatga aaacatattt ttttgagatc aaaataagag cggaccccgc ctctctgctg
    12481 cgagatgcac cgccaaaatg ttatcaagag gaataggagg aagaggcgtc gaggatctcc
    12541 ccctctcaat cgtaccccta tccgatcata atatcgtcta tcctacacct caaagccccc
    12601 ccttcgacct tcagaggatc tacttctcca ccttctctag gtcgctggag aggtcgcctg
    12661 aaaggtcgac gtcgtagaag tctcccgccg tcgggagggc catgaagatg ttgggcgtga
    12721 cgtccagggc cacctcggcc ctatccaccg agaggtcgag gatgtggccg cccgccttcc
    12781 gatcgtcggt gatgaagtgg aggtggtagc cggggacgtt catcccggag gctaactcgg
    12841 ggcaccagaa cccgacgacc gtcccggagg cgtcctcaaa ctcgaagacc gtctggttgg
    12901 cggtgacgtc gacgaggagg gggtagggcc tctcctgggc ggggacggac ctggtcctga
    12961 cgtaggggaa ggtcccatcg atcctgatgg cgtagaagac gttcttcgag gggaagctcg
    13021 ctccgaggat ctccttcagc tcggagaagt tcgccttccc gacctcgaag acctcgtcgg
    13081 ggtcgaagaa ggtgacggtg gcgaaggggg tggtggcctc gtcggggacg acgtatgcta
    13141 tgccatcgac cttcacctga tagcactcgc cgtcgacgac cagcatctct ccctccagct
    13201 tatcgaaggt ccctatgccg aagtcgccct tctccttcag tacgcttatg gggaggatcc
    13261 cgtcgtagac ggccaagagg agggcgtcga tcgtcgagac ctgaaagagg acctcccggt
    13321 cctggtctat cgtcgggtgc gacgagctcg ccccggcggc tgcccccgag gtgacggcta
    13381 gggccgccat gaggagggca gaggctgata tcagagaatt gaatctcata gcgaagatca
    13441 catcctgaga gggtcggctt catcggtccg ccaccctctc tagacctcca ggaccggata
    13501 ttaaataagc tggtctatcc ccggacgggg cgagggcttc cagggaaggc gatcttgccg
    13561 ggccacccga accgatgaag gaccttaggc ctcaattcgg cgcgacccct ccggctgaag
    13621 ggggtgaatc cgcgcgccgg cggaagcccc cttattccag atccggctct atctcgtcgg
    13681 cggggcagac ggtgaccttg gtgtaatcat cctcgcaggc ttcttccacc gtctcgttcg
    13741 agagcccgtc cccggtgcct gcaatattcg cgtcttcacc agcctccggt cccgtcgatc
    13801 tctcggcgca gccggagagg gcgacgaggg ccgccgccat catcagagcg atgatcatgc
    13861 tgacagatct caccaagtag attcctcctc aacctcctgg gagctttcag acgaataatc
    13921 ttttggtccg gctccctcgt cccggaccgt cggcgacaag atcgtcgaaa aggtctcgaa
    13981 acggctttat cgaaggggcg atcacccctc cccgaacgat atcgaacacc atctatccgc
    14041 ctcgcggacg ataaaactag aaggacggtc ttcatcttga gagaatatct gctgggaaac
    14101 gtcgccatcg ccaagggcat cctagaggcg ggggcggggg tcgtctccgg ctaccccggg
    14161 accccctcct ctgagatcgt ggactacctc gcccccctcg ccaaggagct gggtatccac
    14221 gtcgaatggt ctgtgaacg
//
LOCUS       DASM01000015            2834 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_92240, whole genome shotgun sequence.
ACCESSION   DASM01000015 DASM01000000
VERSION     DASM01000015.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_92240"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    217..272
                     /estimated_length=56
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          500 a          907 c          761 g          610 t
ORIGIN      
        1 ttaggtgcgc gagccctcgg gaactatgga tcgctgccac ctcacctaat cttctctaca
       61 aaatactcat cttgtatcac ctcttatatc gcctatagat ttgccggttg ttactatatt
      121 tgttgattta tattagaagc tatattggca tctgctctat ctcatcgaat aggtattctc
      181 cttttcgccc tcccagcagc acctcgaact ccggcgnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcaggcg gtggatacgg cggcgtccac
      301 ccccaggttg atgagggcgt cgggctccat ccggtcgagg tagacgagaa ccatcctttt
      361 tccctgctcc tcccccagcg acatcattct tcttgcgagc tccatcctcc gctgccccgg
      421 cttcttcgag acgatgaccg cgatccgttc ggcatcacca gccctggcta tggctccgta
      481 tcggcgcctc agcatcggcc ccgtgtcgat tacggagacg tttccggtca ccggatcggc
      541 ggccaccacc ctctttcccg tggcgagggc gaccccgagg ggatgaaact gaccagtccc
      601 cacgaataga tactcatcga cgtccgcatt ccgggcggcc tcgtagttgc atcccagaac
      661 ctggccgggg tatctcgtcc tcccgccggg ccggacgatc ccctcaatcc ccctcccccc
      721 caggagctcc aggacctccg ggagcttatg gatgtgctgg actgtggtga cgaccccgac
      781 cctgctggtc tttaggagct cggccgcccc ctcgacggcc tccctgacgt cggccctcat
      841 cctcgcctcg acgtagacga ctttatcgtg gagggggtct ccctccagca tctccgagtg
      901 gccgacgtgt atgaccaaat ccgccgccct gcagaggtcg aggtcgacgt cgcaggcgcc
      961 gtagcacggg tcaccggaga cgatcacctc ggcgcaggtc tcctcctgga tatccctcgc
     1021 taaatcggag gccatcctct tcagcccttc ggggacctga actccaacga ccttcgcgcc
     1081 agatctcttg atcagatcaa aggcagggtc gaggtccagg ttgaaaagct cgaacctaga
     1141 accatttgtc aagggtgctc tgaccaacct tcaccgcctc catgagcctt tttaccgcct
     1201 tctccacccg ttcctgggag aagtcccgct cgtcgcatag aaaggccagg atcttctctg
     1261 ggtctggcct cttcgtctcg atcttgtaat cctccgtcac gggaggatcc aggaagagct
     1321 cccggatctc ggcggcgtcc tcgatcgtct cgcccctcgc ctctaggacc ccctccaggt
     1381 tgccgtgctc ttttatcagc ttcagggcgg tcttggggcc gacccgtttc agcccctcgt
     1441 tatagtcggt cccgcacatt atcccgatct caacgagctg cctcctctcg atcccgaggc
     1501 gcgaaagctc ttcgtcgagg cttatgacct caggcatgac ctcgacgtag acgttctttc
     1561 ccgggagctt cctcttgccg gtgatggcca tgttcctcac tacccggggc gctccgaaga
     1621 ggagggcgtc gtagtcctgg ctccccacga gatcgacgtc gccccggatc gccatgaagg
     1681 cggcctgggc ttcgccctcg gagggggcct gaaccgctgg aatccccatc gcctccaaaa
     1741 gcctcatcgc gtcggcgatc atctcgtcct cgaggcggga tgaagcctgg gcgtacttga
     1801 agccgtcaag ccccacctcc ttcgcccgct gccacatctc ctcggccctg gaccgcgtct
     1861 cggccctctg gtcgagggtc tctcccttga aggagggagg ctccccgtcg aagacgaaga
     1921 cgaccttcgc ccccgcctcc atcaggttgg tcatccgata gaggatcccc gagaggtggg
     1981 atgtggtcct gccggaccca tcccggaggg gggtcccatc ctgctgcctg attatgctga
     2041 ggaactggta gagggtgttg aaggcgtcca ctgcgatcca acgccccgac agctcctcca
     2101 gctctatctt ctccctttcc aggagatctc ccaagtcgac tcccatcaga ctccgacctc
     2161 aatgtcttct tatccatccc ttctcatcag atccgtctca tcggacctat atggtcccaa
     2221 acttaaatat tagttgaacg agtcttagat aaagactgca ggttggtgat gacgatgatc
     2281 gagataaccg atgtggccgc cgaggtattg aaggggaaca tgatccaggg gagggtcgtc
     2341 aggatgatcc tggctgccac cgatgccact ggggcaaatt acgtcctcgc ctggggagat
     2401 cctgccgagg atgatgtcgt cttcgagagc aacgggataa aggtccacat ggctcccgag
     2461 gacgccgaga tcctgggaga cagccccacc atcatagact acgttgatac cgaagatctg
     2521 gggacgggct tcatcatcga gggtccggag gccgaaggat gcggatgcgg ccatgatcac
     2581 gaccacgatg atggaagctg cggatgctga ccttctgaat cgctccggcg ggctgacggc
     2641 cgccgctaat ttttcttacc atcgaaaaat agtcggttga gggggatgct ccccctcaac
     2701 ccgctcctga tgctatcctc ctcagatgag ggggatgtaa tcgacggcct cggggttggc
     2761 gagggcggag gtgtctccgg tggggtttcc gagggccttg gccttgatga cccggcgcat
     2821 gatcttgccg gacc
//
LOCUS       DASM01000016            8993 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_223391, whole genome shotgun sequence.
ACCESSION   DASM01000016 DASM01000000
VERSION     DASM01000016.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8993)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_223391"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1668 a         2737 c         2799 g         1787 t
ORIGIN      
        1 tggtgttgga tatgcaggag gggttcggac acctcaccac ccctactatc aggtctggca
       61 tgtctacggt gtacttctcg acgacccgct ggtcccggat gatgttgatc gtcgccccgg
      121 gggctatgag ggagatcttg ttcacctcct cctccttcag ctcccggtcc tccaccttca
      181 cgatgtcctt tttgccggac ctgcggctgg agacgttcat caggacgctt atcgtcgcct
      241 ccgtcgtccg atctatcccc aggatcctga ggacgttgag ggcctgcccc ggcgggatgt
      301 ggtcgatgac ggtcccgttc ccgatggggg tcaccctcag cttcatatcg gtctcagtca
      361 acgcgattcc cccatcagca tcttgaggag ggccatcctc accgggacgc cgtagaagga
      421 ctgctggaag tagacggcgt ggccggtccc gtccacctcg ggggcgatct cgtcgacccg
      481 ggggaggggg tgcatgatga tcagcccttc cttcgctcct ttcaggctct cgggggcgat
      541 cctgtagctc tttgccacct tggtgtactc gtaggggtcg gggaacctct ccctctggat
      601 ccgggtgacg tagaggacgt ccacctcttt gatcacggat tcgaggtcgt gggtctcccg
      661 gacgatcgtc cccttccccg ccaggtccgc cttgatctgc cccggcatct ccagagtcgg
      721 gggggagacg agggtgatgt cggcgccgta gagggcgagg gcgtaggcga gggagtggac
      781 ggtccggccg tacttcaggt caccgacgag ggcgatcttc aggtcgtcga ggctactctc
      841 cttccggatg gtgtagagat cgaggagggt ctgggtgggg tggtggccag cgccgtcccc
      901 ggcgttgagg acggggaccg gcgagaactg ggccgccagg cgggccgagc cctccttggg
      961 gtggcggatc accagggcgt cggcgtatcc tccgatcacc cggatggtgt ccgccaggga
     1021 ctcccccttg gcgatcgccg agccctcggc agggcccagg ttaagagtct tgccgccgag
     1081 gcgcatcatc gccgtctcga aggagaggcg ggtcctggtg gacggttcga agaagaggag
     1141 ggcgaggatc tttccggaga ggacgtcgca cctccccccc cgggcgtagg gctccagggc
     1201 ctcggcgagg tcgaggacgg agtcgatctc ctcccgggag aactctcgca tcgagaggac
     1261 gtgcctcttt ccgaacatgc tcgggtcacc gctccttagg gatataacgc ttctggatca
     1321 gaagaacttg gaggcgaggg tctgcctcgt ctcgcaggac tccaggacgg ggcacctttc
     1381 gcagggagcc tcccgcggcc ggtcggggag ttttccctcc cggatcgacc tgacccggtc
     1441 ccggatcctg aggaccctgc gccggtccgt cggccggatc tccacctccc ggacctcccc
     1501 ggaccggagg tactcgacga gcccccggtc gaccctcgcc tccagctcct cctccaggag
     1561 catggagtac cccgcgaggc ggagccggtc ggaccgccag acccccgcct ccggtggaag
     1621 gtcgaccctc aatatcgacg ggatcctgag gtgattggag ccatcctcag cccctcggga
     1681 tacgacccgg tcgaccctcc cggagaggcc gagccgatcg gaccggagct ccacctccac
     1741 ggtgctgggg ttgatctcct ggagggagag ctccatcttc ggcgccacct ccgccgccca
     1801 ggcggccacc tcctcggcgg cggcctggag gtcatgaggg tttatccgtt cgcgatagat
     1861 cgccggaacc tcgaccagcg ccttctccag ggcctcccgg agccactccc ccaggtcggc
     1921 cgcctcctcc gggccgatct cggagaggga tagggccacc tccctcatca gaatattttt
     1981 agggatttcc gcctcgggat ggaccctcct gcccatcgcc tggaagtaga ggagcctggg
     2041 gcaccggagg tacagggata tctcggagat cctcaccggc tgagccatgg taagatcaat
     2101 ggtcctccat atttaaaaag atatggaata tatccaattt tcagcaactg ataaatcctg
     2161 gaagcatcat atccaccgac gaggggagtc ggcagcttgg aggtaggacc ccccggggga
     2221 agactatgag ccataagaaa gccgctgtca tatttcctca ccacctctat agagatcatc
     2281 cgtccctccg ggacccggag atctcgacgg tcttcctggt ggaggaggct cggttcttca
     2341 ccgatttcaa cttcaataag aagaagctcg tcctccacag agcctccatg aggggctacc
     2401 aggagctgct tttggagagg gggcttgagg tggaatacct ggagaagagg ccgaaggaga
     2461 cccttccggg cctcttctca gccctccagg agattgggat agggatcgtg cggacggccg
     2521 agatcatcga ccaccccctt gaagcatcct tcttcaaggg gctggaggcg aaggggatca
     2581 ggatcgaagt agacgactcc ccgggatttc tgacctccag ggccctcgcc cccgacctcc
     2641 tccgggggag ggggcggatc tccatggcct ccttctatcg gcggcagagg ctgaggctga
     2701 aggtcctggt ggaggatgga aggcccctgg gcggaaggtg gagcttcgac cccgataacc
     2761 ggaggcgcat cccgaaggag gccgaaatac cgccgccgcc agccctggaa ctgagcagca
     2821 tcgtcaggga ggcgaaggag tacgtagaag agcggttccc caataacccc ggctccgtcg
     2881 atgggttcaa cttccccacc accttcaaag aggcggagct atggctccag gacttcctcg
     2941 cgaggaggct cgcctccttc ggcgactacg aggacgccat ganncccttc ctcttccact
     3001 ccctcctctc ttcctccctc aacataggcc tcctcacccc tggggaggtt ctggacagga
     3061 ccctcgccca tgcatcggag gtcgaagtcc ccatcaactc cctggagggg ttcatccggc
     3121 agctgatcgg ctagcgcgag ttcatgaggg cggcctacct gatcgagggg gatagggaga
     3181 ggagatcgaa cttctggggc caccacgaga agatcccgcc agcgctgtac accgggacga
     3241 cggggatttc ccctgtggac caggtcgtcg gccgggcgat cgatcactct tacgcccatc
     3301 acatcgagag gctgatggtc ctcggaacct tcatgaacct ctgcgagatc gaccccgccg
     3361 aggtttacag gtggtttatg gagatcttca tcgactcctg tgactgggcg atggtcccca
     3421 acgtctacgg gatgtccacc tacgccgacg gagggctcat caccaccaag ccctacatca
     3481 gcggctccag ctacctccgg aggatggggg actggccaca aggcgattgg gccgagatct
     3541 gggacggcct cttctggcgg ttcgtccacc tccaccggga cgtcttcgcc gagaacccca
     3601 ggactagggt cctagcccac cagctcgatc ggatcggcga agagaggctg gagaggcacc
     3661 ttgaggtggc cgaggagttc ctccgctcct tcaggagagg ggatcgttca aagggggagg
     3721 ttagggagga tgaaggcttg agccgtaacg gtgctcatcg ggttttataa gatccgaaaa
     3781 cattcatata atttggattc gaagttggta gggatctggg ggttatcatg ctgaatatcg
     3841 tcatccaagg tcggagcagc cggatctggt ctggactttc gatcgccgcc atatggctca
     3901 ttgggatctc tcttgcggtc cccggagggt tgggggagga gggctcgggc tacagcctcc
     3961 ccctattcat ggacgaggtc gagaacttcg atagcttctc ttccagggtg cccctgagcg
     4021 aggcggctat aatgaggctt gagaggaacg gatttgtggt gatcgtcgac cccttctcac
     4081 cggtccgaga tgaggtcacc cccatctaca ggatcctcaa aaaccacgac cttccgatct
     4141 ttgtaacctc cgactccctc ctccatacat accacctcca gttcggcgag accctgagga
     4201 tggtcgagga ggaggagttc tacgacgacc tatggactct gaccgaagag atgctcgttc
     4261 gggcggaggc cgaccacgag gcctcctccg gcctcgcgaa ggaggcggcc aggaggaacg
     4321 tcgccttctt ctcgacggcc ctcggcctcc tcgcgccgaa ggaggggcag ctctgctccg
     4381 gcggccccca ggaatgtgac gcgggacgct ttgatgggat gtacccatac ttcaccccgg
     4441 aggagctgga ggcgaagagc ttcgaggtgc caccagccgt cagggaggtg gtcgaggccg
     4501 agctcgacct catatacggc caggccgggt cagcaacgtc cccgatattc ggctatgacg
     4561 aggactactc ccagtacagt ccccggggac actacacccg gtccgagagg ctgaagaact
     4621 acttcatggc tatgacctgg tacgggagga tgagcttcct cctcaagggg tgcgaaggcg
     4681 gctgcctcgt ctccgaggag gaggcgaggg tccagaccct ggcggcggcg atgatcgccc
     4741 aggacctcct ggaagacccc gacaccaagg agctctggga ccggatcagc cgagtcacct
     4801 cattttacgt cggttactcc gacgacctgg ggccgtacca gtactgcgat gcgatcgacg
     4861 ccctcttcgg cccggcggcc gaggcccgag acctctctga ggaagagttg agcatcctca
     4921 aggcggagct ggcttcctac agccccccca ggatattcgg cgggaccggc tcggatagtc
     4981 cggcctgcat catcgaacct cccttcaccc cggaggaggc cgacctctgc ctcgccgcca
     5041 ccgccggatt ccggctgatg gggcagaggt tcgtccccga ctcctacgcc ttcacgaggc
     5101 tggtatttcc tgccgtcgga ggctacaccg gcgagggcaa ggccttcacc ctcggcccct
     5161 acggccgctc cttcccgagg ggcctcgacc tgatggcgtt cctcgggtca gagagggctg
     5221 acgagatcct ggaggccctg gaggactcaa gctacgccaa ctactccgcc cggagggcgg
     5281 agctcggagt ggagttctcc tccatctccg aggaggagtg gcagaggaac ctctactggt
     5341 cgtggctctt cgccctgagg ccgctcctcc aggagcccga agccagagcg ccagccttca
     5401 tgcagacgac cgcctggcag gatcggagtc tgacgacggc cctcgcctcc tggacggagc
     5461 tccgccacga tacgatcctc tacgcgaaag agagctacac cttcgtagcc agcggtcttc
     5521 cccccgagga gatggtggcg aaggcgggat acgtcgagcc ggtcccccag gtctaccgaa
     5581 ggctccggga cctctcccgg atgagccgcc aggggctgaa ggaggagggg ctcctcgacg
     5641 agccgtctcg aggccgcctc atgagcctgg aggcgaccct ggagaggctt gaggagatat
     5701 cagaggcgga gctggcgggg cgggagctga ccgatgacga cctccagttc atccgggata
     5761 taggcgagag gctcgacgag gccctcctgg gggtggacga gaggtcgaag aagacgacga
     5821 tcgtcgccga cgtccatact gaccccaaca ccgatatggt cctggaggag gcggtaggat
     5881 acgtgaggat gatggtcgtc gcctgccatc ggcccgatgg gggggtcttc ctcgcggcgg
     5941 ggccggtcta cagctactac gagttcaagc agcccgcctc ggagaggctc accgacgagg
     6001 gctggaggga gatcctggcc ggagaccctc cccaggatcc ggagtggacg gcgagcttcg
     6061 ccgactttct cccctgctct gttctgaaga cgaaggatga ggggagaatg atcgaaggcg
     6121 aagaggcgga tgaaggcgcg gatggcctgg cctccgacga agctggcccg gcgggcgatt
     6181 ctggaggaga aacggccctg ggcgccgtca ttcgcctcga ggagttcgag gtgaggatcg
     6241 gctggaacct ctcggagggg tggtacgccg actgctccat caccctccga aactccgggg
     6301 acgccgaagg gaccgccaat gtactcctgg aggacggcga ggggaagatc ctccgggagc
     6361 tgaagattcc ggtccccccc aactccaccg tcaccgagcg ggcgaaggtg gacatatccg
     6421 ggaggggccg ggaggtgaac tcgatactgg tggggtgaag gtctccttat gaggggccga
     6481 cctccgccaa cggctttttg gcctgggttt cctcgaacac cacctccctg tagacgtaga
     6541 acctgagagg atcctcaggg gtgacatcgt cctggtttgc ggtcttaacc ctgatatccc
     6601 ccatcaggtc ggcgtccgag ttcttgtaga ggccgatctt gttcttttcg ttgttcatcg
     6661 ttatgctcat ggccgtgggg tccaccatct ggaccttcat cctgtcgaag ttcgtcccca
     6721 ccttgatatc gatcggcgaa tcggagatct gccatatccc gttgacgatg accatggcct
     6781 ggtcagggtt gctgaaagca tctttgaagt ggacggctat gacgacgacg tcttcggctc
     6841 cgttcagggt ccttttgtag gtgtaggtct tctccgggat ggtggcgccg tccttggagg
     6901 gctcgaccgt cgcaacgtcg atcgtctcgc cgttcttttg gagttcgagg ctgacgctct
     6961 tggtcctctg atctatcgcc ttgacggaga gctcgtaccc ttcggcgagc ttgagggggc
     7021 tgcttgaggt gagggtgatc tccgagtcgt cgcttatgag cactcggctg atgaggtcct
     7081 cggcgatcag gttggggtca gtggattcgt agtatagggg ggcgtcataa ggagagcctt
     7141 cagcgtagcc tgcaaagtgc tcctccccga ggaagcctat cgtccaatag cttccccaag
     7201 tttcgaagtc gaaatccttc aactgggccg aagtcgtgta gacgacgcca acgggctctt
     7261 caagggagtt tccgtggatt gtcgtggtca gtgcctcggt cccgaggttg ttgtcgacgt
     7321 cgtagtagaa cccggcgaag ttggagctct cccatgtggt cacccctccg tcgatgacct
     7381 cggagacgct tcctctgatc tcataccttc cagggcgggt gatctctttg aagacgtaga
     7441 atctcatggg ctcttcaggg gtgatatcgt actggtcagc ggtcctgatc ccaaactcct
     7501 ccatcagagg gatctccttg ttcttgctga ggactatccg attgtcctcg ttatccatgg
     7561 tgatccgcat ggattctgca tccaccgagc tgattctcat cttgccgaag gatgagccat
     7621 cttcgatgct gatggcttcg tcggagatct gccatatgcc gtttactatg gcagcgtcct
     7681 ggaggccgcc ctctgtcccg tggaagaccg gccccttgaa gtggacggcg atcaccacaa
     7741 cccctcgcga aattccgaga tctctggtgt agatataggt cttatcggcg atggtggcgc
     7801 cgtccttcga cggggctacc agattggagt cgacgacctc gccgtctttg ctcagctcaa
     7861 gccagatgga cccgtaggcg tcgatcccct tgatccccag ctcatagccc tcagctagcc
     7921 ggaggggagc ttcttttgtg atggtcctct cctggtcgtc gtcgatgagg atcttcgata
     7981 gctcctctcc ggccatcagg ttgtcgtccc ttgatctgtc gtagaggtaa ggctggcgcc
     8041 agtcatcgta cctctcccag gcgtatcctg cgaactgctc ctctcccaaa aaggagattt
     8101 tatagtacat accccaatcc tcgaactcga agttggagag ctgggccgtc gtttcgtaga
     8161 cgacccccat cggctcgtcg agggagctgc cggtgatcat cagggtcagc ctctcattgc
     8221 cgatgttatc gtcggcgtcg tagctgaaac ctggaaagtt ctgggggtcc caagtataag
     8281 tcgcgccgtc gtaggccact gccacggagc ctcgaagctc gatcctttcg gcggcggttg
     8341 cggaggtcgt cacgaaaaga aggatcatga cgaaggtgaa gaatgccggg gttctcatag
     8401 tatcgtaatt tatttgccag ctataaagca gattcgtcct tcattaattc tgctatcaag
     8461 catcctcacc gatcttacag caacaaaacc ccttaaatcc cagaagaacg atataacccc
     8521 aaagatcaag cttctctcag ggtgtaaatg gaccatgaaa cgaaaatggc agcacgacag
     8581 ggggctggag gcgaggatgc tcttcaccat gctactcctc ttcctggtct acatggcctt
     8641 cctggccatc ttggcatacc agggggcggg gatggtgacg atgctcgcct tcgtcatcat
     8701 ttttatgctc gtccagtacc tcttctccga caggatcgtc ctcaagagca tgggcgcaaa
     8761 gatagtctcc gagacggagg agccccagct ccaccagatg atcacgaggc tctgcgccct
     8821 ggcggacctg ccgaagcccc ggatcgccgt cgtcaaaacc tcgatgccga acgccttcgc
     8881 caccggcaga agcccgaata actccgtggt ggcggtgacc accgggatca tgcggcagct
     8941 caccccggcg gagctggagg cggtcctggc ccacgagctg acccacgtca aga
//
LOCUS       DASM01000017            7479 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_188288, whole genome shotgun sequence.
ACCESSION   DASM01000017 DASM01000000
VERSION     DASM01000017.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7479)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_188288"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    2382..2403
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1418 a         2356 c         2105 g         1578 t
ORIGIN      
        1 agtcgaggaa ccatattcca taggtaggat cgaatacccc cacagtatcc ttgccgtccc
       61 cattccagtc gccagtaact ggtttgtggg ttggggctcc atagtagagg gccttgtctg
      121 cgacgccgtt gttgtcgtag tcgaggaacc atattccata ggtcggatcg aaaacgccta
      181 ccgtatctct tccatctccg ttccagtcgc cggtgaccgg gatgtgagtg ctggctccga
      241 agtagacata ctgatccgcg attccgtcgt tgttgttgtc gaggaaccat aggcttgtgg
      301 cgggatcgaa tacaccaacg gtatctttgc cgtccccatt ccagtcgccc gttaccggct
      361 tatgggttgg ggcaccgtaa tagaacgcct tatcagcgac gccgttgttg tcgtagtcaa
      421 ggaaccacag gccattggtc gggtcgaata cgcccaccgt gtccttcccg tattctggga
      481 gatcgtactc cagatcgagg gtctcccagt aaaacgcgta gtatccctgt ccacccaagg
      541 tgaagtagtt gtcgtagttc atgttcatgt tgacgcggaa gagcccgttg gggcgtccag
      601 ctgttgtccc ccagctgttc aacatgatcc agtatttgtt gtttgggtcg ttgtcgttgt
      661 agcccacaca gaggacgtca tggcctgccc cctgctcatc attccaggcc ttgccatctg
      721 ggaacgtcgg gttgtataaa gcgctctcgg gctgattgag ccaaaattgt ccgaatatgt
      781 tccattcgtc caaagtcgga aggaagaatg agaaggttac agccttattc tggttcaaga
      841 cgttcttgat gttcgatatg gcagtggcgc ttccaacacc atgggtgagt atggcctgcg
      901 cctcaattgt gtctatggga tagttgggtg tggtcgttat cacccccgcc ggtacgctgg
      961 tggagcccat ctcgcaagtt ctgcccccgt cctgccaatg accattggcg ttggaccatg
     1021 gaatagcttt acctgtgccg gcataaaaat tggccaaatc taccaaccaa ttaccgcagc
     1081 aagcccaatc agatccactc cctcccaagt agttggagtt gaggtattgt atcgaaaggc
     1141 ggtccttgat cccctcttca acggacagtc cgatctccat aaccccggtc ccagcccaga
     1201 cccagcagtt gccacaatcc cactggtctc tctcagcagg aatgtactgg agatgattca
     1261 gcaggctaag ggagccagta ggcgatatat caagttctgc tgagatctca ggatcgatct
     1321 cagcgagggg ttgcatcttg aatacctcta tccatctctg aagcgtttct ggggatgggc
     1381 gcatgagggg atactcgcgg atgcttgtgg cgaggaccgc cccctcgaag ttgccaaagt
     1441 cgtacctttt tccgtcctta tccaccagag ttacattata cgatcctcca cctggagctg
     1501 tctggttcca gcctggcata aggtcttggg cgacgacgta ttctccggca tcgaggccct
     1561 cgaagatgta ctctccgagc tgatcagagg tgacgttgag gacttccatt tcgtcctgca
     1621 taagccttat cgtccatcca ggaagccctg gctctccctc gtccatgaaa ccgttgccgt
     1681 tcaggtcgtt gaaaaccgtc ccgtttaagg ataaaatgtt agaaaagttt acggatgctt
     1741 ctgactcatc tgtaccgagg gactgttctg ctgaaactgc tggagcctga aagatcaata
     1801 tgaataaaac tcctagggat agcagcatta gacgattgat cgataatctg ttcatactta
     1861 cctcaccacc ttaatttatc ttatcacttc ttctctcttg ttctataaat tcgtttctct
     1921 caattcatca aaagtatgta attaactcga tttatgtttt ggtaattaaa ttctgcgggt
     1981 agcattatgg atataataaa taatattatg atcaacaggc ccgtttcagt tgagtaattc
     2041 taataatatc ctcatattga ctcaccgtat tctagtgtgt ttcaacttat atcagaagat
     2101 tcgcttgatt aatatatatt gtaaaatatg taaaaactta tgtagctttt tacgtattct
     2161 tctttggcga atatcaaata taatctctga ttgtatcaca taatgccgat gaataaatat
     2221 taggaatccg cctataaggc ggattctata ctaaattttt aaccaatcgc ctgtaacagg
     2281 catatgggtg ctggctccgt agtaaacgaa cttatccgca acaccatcta tgtcgtagtc
     2341 caagaaccac agaccagaag ccggatcgaa tacgccgacg gnnnnnnnnn nnnnnnnnnn
     2401 nnncggtatc cttgccatct ccgttccagt cacctgtaac tggtatgtgg gtgctggctc
     2461 cgaagtaaac gaactcgtcg gcaataccat cgttgtcgtc gtctaggaac cactgacctg
     2521 aagccggatc gaatactcct atggtatcct taccatctcc attccaatct cctgtaaccg
     2581 gtttgtgggt gttagcccca aagtaaacga acttgtcggc agcaccatcg ttgtcgtcat
     2641 ccaagaacca caatccagaa gctgggtcga atactcctat agtatccttg ccatcaccat
     2701 tccagtctcc agtaaccggt ttgtgggtgg tggccccata gtaaagggct ttgtcagcag
     2761 ctccgtcact atcgaagtcc aagaaccaca atccagagtt ggggtcgaac acccccaccg
     2821 cgtcctcccc cttcctcccg aagtcgatcg ccgcaatcac gatcggcccg gtgaaggaga
     2881 gccccgccat ggggacaggc tcgctggagg catccagctc ttcatcctcg tcaggggtga
     2941 cggccggatc ggccagctcc cctccgggcg ggacctcgcc gtcggcgggc atggggacca
     3001 catcgggcgg caggacctcg tcggccatca ccgagagggg cgtctcttcg gggttgaggg
     3061 cggcctcgac atcggcctcc tggggccgaa cctctggatc agcgccgccg gccgcggtga
     3121 cgtaccccga tggggtcgtc ctcgccaggg cgaaggcgtc cttggagatt ctgaggctgg
     3181 aggcctctat caccaggatg tcctccttcg gccccaggat ggcgttggtc accgccccgt
     3241 cgtagcggag ggggtcagat accgtgtccg cattgaagtg gaggccgaag aggtctccga
     3301 cctggttgat gacgtcgttc tcggccaggg gcgcggggcc atcggccggg cccatcggcg
     3361 cgatcggcgg gagccagtcg acgccgtcgc cgcccaggag gaggaggccg cctccgttgc
     3421 cgctggcgag ccagtcgacg tactggaccg ccgccttgac gttatcctgc tggtagatga
     3481 ccgagttctc gaagaggtcg ccgtccccgg cggctatcac gcggccgcgg ccgacgaact
     3541 ccttgatcgc cgccagctca tcggcggtga aggcggcccc ggggttagct atcaccacga
     3601 cgtcgtagca ctcgaggagc tccgccgtca ggttcgcctc gtcgctgctg tggacgaggt
     3661 agccgttccc ggcgagcctg ttgccccact ccgagtagtt tcccaggagg gcgtatacgg
     3721 gtccgtgccc ctcgtagaag aggaccttcc cgtcgatcct cggctggtag gcgaccgcct
     3781 cgtagccgat gagcgagtcg cccatcttta tcctgaacca gccggagtcg ccccatccgg
     3841 cgccccagga gttcttgcag atccagtagt cggtgctgtt attcgtcccc cagccgagga
     3901 tgtccaccca ctggctcccg accagggctc ccgcggcgtg ctcgtagata tcgtcgtcgt
     3961 agaggtagaa gtcctcgtag accgccatct tagacgagac ggggccgccg aagaggacct
     4021 cctgctggat cctggggacg tcgatcacat ccggctcccc ggagacccaa caccacccct
     4081 cgatcttgta cttccgggcg tcccggtcaa agcagctctc ggtgcagggg acggtgtcaa
     4141 gcccccggta ccggaagcag gcgtcgtcga ccgtcccctg gcagaggagc cagtcggcgg
     4201 ttaggttgct cgatccattg atgcagctcc ccggcccgca ggagaggagg tactgctcgg
     4261 agaggtcggg gcggaggttg gggttattgg ccgcaagctt catccgcgcc tccaccgccc
     4321 ccagggcggc gaaggcccac cccgagccgc agttcccctg atcctttatc ggggtcatcc
     4381 agctctcccc gctgacgttc ctccagtccc actctccagg gacggcgtcc gtcgatggcc
     4441 cgcctccggg gaggatagtc agaacctcca tcgggaagtc ggccgcgccc agaccgggcc
     4501 gcagctccgg cggcaggatc gataccgggt tcagccccgc ctcccagctt gcgcctgcct
     4561 cctcgatcgc ctgcctcacc ctcaccagct cggcgatccg ctccggatac gtcgcgatga
     4621 ccagggctct atcctccggc gagagggtcg ccagcatctc ctcgatggcg gcccagtccc
     4681 cgccggccgc cagtaccgca cctttcatct cttcgagccc cggctcctcg gccgccgcgg
     4741 cggctggaga aaaggccaga atcgagacga agatggcaac agccaataca gctatctctc
     4801 tcatctcacc actactccct actttgagat ctgataggtg atcttacatt tactgattat
     4861 aaattatgcg ctccccccgg aggcacccct gcaccaagtc cccttctcga tattaaatcg
     4921 gatatatttg ggatggtgct tcaaaaatcc gtggatctcc agacccccga tcccctcctc
     4981 gtccccggag agatcgtcac ctccaggggt tccgaccacc ccgaggccgc ctcgagatcc
     5041 tcggccttca ccctgacggc gcgggtcccc ggcgcctccc agcggtgagc gaggctgacg
     5101 gtcgcccccg aatcgaagaa ctccgtctcc gagaaggtgc cgtcccccca gtcgtaggtg
     5161 tacttcaccc ggttgccgtc ggggtccgac gaggaggtcg agtagctgta ggccgtcccc
     5221 gtccggccgc tggcggggcc cgcaggcctt ccgggcgtct ccgggggggc gttcggcgac
     5281 gggggcccga cccgtagggc ggggtctccc aggaggagga acatcttgac gttgtccgcc
     5341 gcataggagg tggcgccgta cttcgccatc accttgacct tggcgtcgtt gaccacccag
     5401 ccgaggtcgg atatcccccc gttccccatg gcgtcgaaga ggtatcggtc gaagtcgtgg
     5461 ttcggctccg tccaggaggg cctcgatgcc cccagaaagg cggcggcaga gccgtcgtcc
     5521 ttgacgaagg cctcggcgag gcaatccgcc gcctggtcga gggcggcgtt gtagcaggcg
     5581 atgctgagga ccgcaggaaa ggcgaccccg gccatggcgt gggcctcgct ggtcccgaac
     5641 tcctccccgg catagttcca ccccggcccc cagctggtgt agctcccgtg cccccggtag
     5701 ttgacgatcc cgaccccgga ggcggcggcg agaacgtcgg cgttggtggc tccgtccccg
     5761 ccttgggagg gcggcgatcc gtaggcggtc gcaaaggaga aggggctcgc ggaaggggcc
     5821 gtcctgatcg actccttgca cccctgatac ttccccggag cctcctgggc gtgggcgatg
     5881 aggaggacct tcgaggccca gtctcctgtc ctggcgccgg tctcataggc gaggatcttc
     5941 gccacctgct ggccgacctc ggcgtcgttc ttcgccgtta tcctgccgac ggccacctcg
     6001 gggtagaggt cgacccccgc gacgcaggca taccagtagt cgctggggat cgtcatcccc
     6061 ggaaagtagg agctgaagga ggaggagggg tcccagttcg gcctccaggg gagccttccg
     6121 atatcgccga ccaggaggag gtactccagc tcgggatggg cggcgtagac gctcgccacc
     6181 acctccttca ccgcctccgc cgaaggcgtg ctggaggacg aaaagtagca ggagaggacg
     6241 ggaacccccg ccgcctcccg ccgctccagg agggggcgta tgctctcata gctgctctgg
     6301 ccctcatgcc tgatcgagag gtacttcacc atctcgggac tctccgcccc gtcgatcccc
     6361 acccccggag ggaggtccgc ctccccccca gcgggctcgg ggcccctcac ctcgaggtcc
     6421 agaaggtggt agttggatat cacgtcccgg tagatcctcg caaacttcgg ctccgccgtc
     6481 tcttttgcca ggaccccccc ctcgtagtcg aggcggacga gaatcctgtc gtagaccctc
     6541 agctctcccg tcgccgggtt gaaggccacg gggtttgcct ggagctccac caccaccagg
     6601 tcccgccaga tcccgggggg ccccacctcc accagctcct cggggtagaa cccgtcccgg
     6661 gagtagaact cctcgtcgat caggagccct cgatatcctg agaggccgtc cacctccggc
     6721 ggctggaatg ggtggaccct gaaccccggg tatgtcgagg aggaggcctc gaggacggtg
     6781 gcgcggaccg ccccccccgc cgggaccgcc accatcgccc ttacgacggg aagttcgggc
     6841 cgtcccactt cggtcgtcgt tcccctctcc gggatcgaga ggaccgagaa ggcctcgctc
     6901 ccggcagccc tctcctctgc cctcaccccc cggaggagga actctacgac cgtcccagag
     6961 gcgtccgagc tggagacgag gacctcggcc cccacctctg ggccgttgag gatccggccg
     7021 cccccacctt cctcctctcc ggccccgcct ccctcttctg aaaggccggg cgccgacgcc
     7081 atgaggtccg ggggaagctc cgcattccct cgaagaagat ggggcccctc cggggaggcc
     7141 aatgctgcgg cccagagcgg catagacgcc acagcgataa taactgggat caactccttc
     7201 acctgctacc ccctcagcca tctgataagg tgaatgctcc cctccctaaa gggaggggct
     7261 tctatcgcgt ttgcgaagag attgcattcc caatctcaga atgttgatag ccgcattctc
     7321 gtcacggtcc atgaccagcc cgcattgagg acgttcatgg acccgatcag atagcgactt
     7381 cgtgacttta agaccgcacc tggaacatag ctgacttgtg ttcctgggat ctactagaac
     7441 cactatcgaa ccgacgcttt cagccttgta cgatgtgat
//
LOCUS       DASM01000018            9188 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_100376, whole genome shotgun sequence.
ACCESSION   DASM01000018 DASM01000000
VERSION     DASM01000018.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_100376"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1917 a         2661 c         2846 g         1764 t
ORIGIN      
        1 atcgcctcgt agaggtcctc ggcggtcttg atgaagtggc tcatccccag gatgacgttg
       61 gagtcctggg ggatcttcat cctcacggtc tcaaaattca tcttcgaatc ctcctcgtgg
      121 gggatagggg gcgagatgac ttataattat ggctcctggc cggctccccg gatggatcgg
      181 gggctttctg ttggtaacga cacttttatt taatcaccgg tggaaaggga acggacggag
      241 agcggcatga gaaagatcga gtactgtccc ataagcatga agcggcttct ggtggagatg
      301 aaggacacca gtgagctgat ggtggatctg ggatattccg cggtcctcta cgacgacgaa
      361 gacctggcga tggaggtgat caggcttgag gagaagatgg acgtctacga ctaccacgtc
      421 cagatggcgg cgatgctggc cgcgaggacc gtcgaggacg ccgaggccct ctccggcgtc
      481 ctccagatcg catcggcggc ggagatcatc tccaacgctg cgggggacat cgccagcatc
      541 gtccttttga agatggggat cccctccgag ctgaagaagg acctgaggca ggccgacgag
      601 acggtgggcc gggcggtggt gggggcggag tcgaaggtct ccgggaggag cctcgaggag
      661 atctccttcg agacggaggc cgggatgagc gtcatcgcca taaggcgggg tgagagctgg
      721 atctacgatc cggagcccgg gacggtgatc gtcgccgggg acatcctctt tgcccggggc
      781 cacgacgagg gggtccccct cttcctggag atgacgacgg ggaagcccgc cgtcccgaag
      841 gtcggccagg agagcgggat cggccagctc gaccgggcgg tagacatcat cgtcgagatg
      901 aagaacaggt cggagctggc ggtgggcctc gcctacaccg ccgttctctt cgacagcgag
      961 gaggtggcga tcgaggtgga ggtgatcgag gaggagatgg acgagatgaa gtacgacctc
     1021 cagcggtggg tcctccgggc ggcgaagcac gtcgacgaca ttgagaacct ccggggtctc
     1081 ctccacctcg cggtctcgac ggagatcatc agcgatgccg ccctcaagat cgccaacgtc
     1141 gtcctccacg atatcgagcc ccaccccatc ctcgccatgg ccatgaggga gtcggacgag
     1201 gtgatcaccc gggtcgagat cgcccccggg gcggagatgg acggcgaaac cctcggcgac
     1261 ctggacctgg agacggagac agggatgtac gtcctggcgg tgaagcgggg cgggagatgg
     1321 acctacaacc ccaagccgaa ggtgaccctg ggggccggag acctgatctt cgcccggggg
     1381 agcaagagcg gcgaggagct cctcctgaag atgagcggct tcgcccccga ccggcccccc
     1441 aggccctgat ctccgatcaa tgagattctg gtcccccttg gaggtgcctt tgcccggcta
     1501 ctttctcctc tggcccttct tcctgaggac tatggtggat gtgacgaagt agctggtgga
     1561 gaagatcgcc gcggcgaata gggattcccc gaggctgggc acccctcggt agtctaggta
     1621 gagggcgtag accagaaaga agaagaggcc tgccaccagc gcctggccgg ccacgctcct
     1681 ccttttgaag ttagcaagga ttctcatctt ggtgaccttc gggctatagg tacttcaccc
     1741 cttcgtcccg ggggctttcg gcctcggcca tcttccccgc cctggtcgta tagagcctcg
     1801 tccaccagac ggtggtgagg ggggaaatga tcaggacgga gacgaccccg ctgaggctgg
     1861 agaaggcggc gctcgccgcc tcggagcccg agaagggcgc ggcaagaaac tggatcgcca
     1921 ccgacaccgc catgacgact agccagatca ggaagacgtc catcttgttc gagcggaaga
     1981 aggcgatgcc ggcctttatc gccccgatag ggccgagccc gtcgacgacc agggcgtagg
     2041 ggaccatggc gaggaggatg gatatgagga ggctaaaggc gatcaaaagg gcgatccaga
     2101 ggaggagggg tccgaattcg ggctcgttcg tctcgaaggc tcccatcagg tcggcgatga
     2161 aggggagggt caggacgagg acgccgacga ggacgacgac catcatgacg acctcggcga
     2221 ggaagaggct gaaaaagtgc cccctccccg aggaccacat atccttcagg gtggtcttgc
     2281 cggagagggt cgccaccttc gccattccta tggcccccgc ggtgaagaag gagctagata
     2341 gcgccatccc caggatggct atgaggatga tcgcggccgc gaggccgagg ttccccgccc
     2401 cctggagaag ctccatcagc tcctgggggt cttcaagatc cccggccgtg gagaagagct
     2461 cgggggatga ggtgaacaat ggatacatca cggcaaagac catggcgacg gccaggagca
     2521 tgatcgcgac tatcatcagc acgaagggga cggccaggtt aaggttaccc ctccacgtct
     2581 caaagcccct cctgaggagg gtgcctatgt cttcgttcat ggatatcacc tttgatctcg
     2641 gagttcgtcg tcggttcagg agctccgcca gccaggagcg ctctcatacc atttaattat
     2701 ttttggcgga gcccatcagt cgcccatctt ccagaggtcg tcgccgacgt ggtggaccga
     2761 cctgaccgct ttgcccgacc cccgcatctc ggggccgggg aggagggcga tcccgacggc
     2821 gatgggcttt cggtgcctct cctcgacgat gaccacagga tcgccggggg cgatcttctc
     2881 gtccgcctcc acgatcccgg gggccatcac gtccgccccg ttgatgatga atctgacggc
     2941 tccggagtcg acgacgacca ggttcctcct gggcctaaac tccagggctc cccggacggt
     3001 gaagaagggc cggtcctcca ccatcatgaa gactggccta cccttcagaa ggatcaggtc
     3061 gaagtcctga tcggtctcgg caacctccag agccagcccc cgcaaggggg aaggatcctc
     3121 caaaaagggc tcgatggcgc tctggatcct cttcgactcg ctccccttga ggtggtgcct
     3181 ggacttgatc ttcataaaaa cccctattcc ccgacaggtt taaatatgca acgtgcgagt
     3241 ctcgccatag ctcttgtatt taccactgga gagaggccga tgtctcagag accccttgac
     3301 attttaaatg aatcgctgaa cgggcccgtg atcgtcaagc tcaaggatgg gagggctttt
     3361 cggggggagc ttcagggtta cgacatccac atgaacctcg tcctcgacaa caccgaggag
     3421 atccgagagg gtgaggactc caagaagctc ggaaccgtcg tcgtccgagg agataacgta
     3481 gtttacattt cgccgtgagg taatatagat gacaaaaggc accccatcta tgggaaagcg
     3541 ccacaagaag acccatgtca agtgtcgtcg atgtggcaag gtatccttca actaccgtac
     3601 caagacctgt gcatcctgcg gcttcggcag gtccaagagg ctccggggct acgtctggca
     3661 gaaatatagc agcaactacg gcggaaactg attcggaggt ccgggttgca ggacaagtgc
     3721 ggcgtggtgg ggatctcctc cgcggatccg tgtcgcaacg tggccaagtc gatctactac
     3781 gccctctatg ccctgcagca ccgtggccag gagtcggccg gcatctccgt ttacgacggc
     3841 aaggtcgtcc ggacccacaa ggccatgggc ctcgtcgccg aggtcttcaa caacccccgg
     3901 atcgaggccc tgggggggcc cacgggggtg ggccacgtcc ggtactccac caccggcgag
     3961 tcggccctca agaacagcca gcctattttg gtgaagtata aggagagctc tgtcgccatc
     4021 gcccacaacg gaaacctcgt caacgccgga aagctgaaag aggagctgga ggcggagggg
     4081 caggtcttcc actccacctc cgataccgaa gtgatggccc gcctcttcgt ccgggcgatg
     4141 atggtgcggg aggatcccgc cgaggcggtc cgggagatca tgaggaaggc ggtggggtcc
     4201 tactccatcg tcctcttgat gggaaacacc gtcatcgccg cgagagaccc cctggggatc
     4261 aagcccctat gcgtcggaaa gaccgatacc gacgtcatcg tcgcctccga gagcgccgcc
     4321 atcgataccc tcaacggcaa gctcgtaaga gacgtccgcc ccggcgagat ggtcgtcgcc
     4381 aaagacgggg acgtccggtt ctaccagctc tcccacaacc aatgccccgc ccactgcgtc
     4441 tttgagtaca tctacttcgc ccgccccgac agcatcatgg acgagaggct cgtctacgac
     4501 gtccgggtga agatcggggc catcctcgcc gaggagcacc ccctggaggc ggatacggtg
     4561 acccccgtcc ccgactccgg gatcaccttc gccatcggct accaccagag gtcgaacatc
     4621 aagtaccggg agtgtctgat gaagaaccgg tacatcggga ggaccttcat catgcccggc
     4681 caggagctga gggagatcgc cgtgaggctg aagatgaaca ccatccgccc gaacatcgag
     4741 gggaaccgga tcatcatggt cgacgacagc atcgtccggg ggaccaccag caggcggatc
     4801 gtggacatgg tgaggtcgac gggggcaaag gaggtccacg tccggatcgg aagccccccc
     4861 atcgtcgccc cctgctacat ggggatcgac atggcgacga gggaggagct gatcgccgcc
     4921 cacaagaccg tcgccggggt cgaggcggtg atcaacgccg actccctggg ctacatcagc
     4981 cacgaggggc tggtccgggc gatcggcgtc ccccgggaga gcctctgcat gggctgcctc
     5041 accggggtct atcccatcga gatccccggg gaggtctgcc gcctcgccca gacgaggatc
     5101 accgagttca tcgaggaata aggccgtcgg cttacactaa aaaaaaccat tttcaggatt
     5161 tgagcacaca gaagcctgat gctggtgttc tacttcagct gctctttaag tattctcaat
     5221 ctcccttcga ttttagatct taattcatct agaccttctt tattgtttag ataaagtata
     5281 actctatgat gcctaagatc aaatggaata tcttccatat gttgagtgat tggaatcaca
     5341 tgttttccta atgtatgagc tatgcccact tcataaaaca catttggatt tttgcctgaa
     5401 aaatccacga taactataga tgaacagcat ataagttcga aaatatcttg aacaattatg
     5461 ctattattcc atatgtcatc agctctttta caatccaaat ctaaacttga acatgctgct
     5521 tttatagttt ctagcacttt atcgaatctc atttcaaatg ggatcataac agaaactaag
     5581 ttagttttga gaggcttact aggaggatca aagaccgaag gtgagaatgt taacacttct
     5641 ttcgcgtcat ccgaatttat tgatgtcgaa atgtaacccc ttacaatttt gcaaagtttt
     5701 acattagatg tctgctcatt tttggtaaca ataagattag ttattttcaa aatttcattt
     5761 acttgttcta tcgctttatc gtggtcatat tgtggatctt tgaaatgaat gggatttaat
     5821 agattttcaa taacctgaac caatttatgc gaaggaagat ctgaatctgc actttcttcg
     5881 ttgtttaatt catccaaaac actcgcaacc cacctcttcc tagtcgaacc gtcatggaca
     5941 taatctaaat ctacacctct gaaaaaactc gttagataaa atgatgatct ataagggaaa
     6001 tactcataag ggggattacc gcaaatcatc tccgccagta catcaatcga gctagtgcct
     6061 attttcatga tgagcacctt aatagccttt accaattgca gtcttttctg tcatgtgaca
     6121 tatataatcc ccttgtattt ttagcctcag caaactttgt agatgggctt ttccgacccc
     6181 ttttaaagaa atcctcacta ccaccatctc cacagccttt ttcacctccc gccgcctaca
     6241 cccacccatg agagcgcttg ccgtgaccgg aaggctcgcc gaggggatgg tgagggaggc
     6301 ggtcggaggc ctcgcggacg tcctggtgat ggatgtggac gtagcctcct tcatcacccc
     6361 gaagatgatc cgggatgctg cgcccacggg ctacgatata atcctgatcc cgggagccat
     6421 caccgccgac ttcggccccg tcgcagcgga gctggaaacg aagatccggc tcgggccgaa
     6481 gcacgccgtc gacctgcggc gggtcttgcc gctcctaggc gaggtggagc tctcgaccac
     6541 cgtcccggcc tgcgtcctcc tggaggagag gatgaggtcg gaggcgcagg ccgccatcct
     6601 ggagctggag gcgggggcgg aggcgccgct tgaaataaaa ggcgtcaagg tcggcggagc
     6661 gagccggatg aaggttctgg ccgagatcgt cgacgcgacc cgcttcggcg acggggaact
     6721 cgtcgagaag gtccgccact acgaggggga gggggcggac atgatcgatc tggggctccc
     6781 tctggacgcc gagcccgtcg acgtgacgcg ggccgtttgc gtggcgaagg gcgcgacccg
     6841 gcttcccgtc tctgtcgaca gcttgcggcc cgatctcctc aaagccggga tcgaggcggg
     6901 gtgcgacctc gtcctcagcc tcgacggctc gaacctggac gatgtgggtc cattggcagc
     6961 ctccgccggg gttccggccg tggtcctccc ggggccggag gcgagcctcg cggagaacct
     7021 ccgccgggcc ctcgccctcg gggtcagggc gatggccgac cccgtcctca gcccgccgct
     7081 tctggggctg gccgagtcga tctgccggta ccgcgaggtc cataaggcgt ttcaaaagct
     7141 gcctctattc ttcggcgcag ggaacgtgac ggagctcctc gacgccgact ctccgggggc
     7201 gaacgccctc ctcgcctcca tgggggcgga ggtgggggcg gcggtcctct tcacccccga
     7261 gtacagcgat aaggcccggg ggagcatccg ggagctgcgg agggcctccg agatgatcct
     7321 cctcgctggt gcgagaagga gcccgcccaa ggacctgggc ctcgaccttc tggtcctaaa
     7381 ggagaagcac cgccgccctg aggaggagat gccggaggag tacgtggcgg tgggggcgat
     7441 ccttcgtgat ctcggggagg ttgaggttga ggtagaggag gagggtggcg gctattcgga
     7501 ccgtggcggc agaggaggcg gaggcagcgg ggtcgaattc ggtggccgcc gctgggagat
     7561 ggaccccgcc gggagcttca ggatcggcgt cgcgaaagga aagatcgtgg tccgccacga
     7621 cgctgtcacc ctggtgggca ggtcggctcg cggcctcctc ggcgagatcg tctcccgggg
     7681 tctcgtcacc cgcctcgacc acgccggcta cctcggccgg gagctggaga aggcagagat
     7741 cgccatcggc cttgggagga gctattctca ggacgagccg ctatttccaa caaagaatta
     7801 aataagatcg gctcgtctcc cgcccccgat gaatcagaaa tcttgggcta ctatcggagt
     7861 ggtcttcctc gccgggataa tgatcttctc cacctttgca ggcttcgtca tgcggggcgg
     7921 ggaccccgtc gtcacgacca cctcgtcggc ctggaacccc tccgacttcg gggtgggtgg
     7981 acggttgatc gattgggact tcgagggcct cggcgacgcc ctggggatgt atccggagga
     8041 cctggtcttc gccttctgga tcaacatgac cgcctccgag aacctcaccg aggcggccgc
     8101 cctcgtcctc ccgcagacca tcgccctcac cttcagagat cagttgaacc tctacccctc
     8161 accgatcgac agcttctcct gggggctctt cggtgacgag ttcgtcgagt tccactggac
     8221 gaggcccgcc ccaatgggtg gccacggcct atcggtagtc tacaacggct accagatgat
     8281 ccccctcgag atgacggacc tcttcagggt aatgggaacc cccgtcctct ttgggagcga
     8341 gccggcagta aagagggtcc tggacgtcct gtcggggcag gcctccacca ccgagggctt
     8401 cgtcctcccc tacgacgagg ccgacgacct ccagatatcg tgcctgggga aggacgccgc
     8461 cgagaaccct agcctaatgc cgcccctggg gggagagtac gtcgagtcgt acctgggcgt
     8521 cagcccctcc gatgacggct tcatcctgac ggcgaagtac ctctccctcc gtggcgaaag
     8581 ccgtgatagg gttcgcgacc tggcggagag ctgggggctt tctgtcagtg aggaggggag
     8641 gatagtcgcc gtctcggggg tagtggagcc cgagaacctg gccgggacgc tgggagcctt
     8701 cgtggttcca tagcgcaacc tttttagctg ggaagcggcc cccatgagct aagccattct
     8761 ggggcgattt ttgcgatgaa tgatgacggg caccgccctg aaggagaata aattatggca
     8821 agaccgatat acgttagatt tgacgttcca gctgacctgg cctccaaatc cctggaagcg
     8881 atcgagctcg cccgggacac tggaaggctg aagaagggaa ccaacgagac gaccaaggcc
     8941 atcgagcggg gcgtagccaa gctcgtggtg gtgggcgaag acgtggagcc tccagagatc
     9001 gtggcccata ttccgccgct ctgcgaggag aagaagaccc cctacatcta cgtaaagaag
     9061 cagagtgaga tcggttccgc ctgtggcatg agggtcaaga gcgccgccgt ggccatcctg
     9121 gagccgggga agggaaagga catcttggac gagatagttc agaaggccca ggccctgaag
     9181 taggggtg
//
LOCUS       DASM01000019           12372 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_2855, whole genome shotgun sequence.
ACCESSION   DASM01000019 DASM01000000
VERSION     DASM01000019.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12372)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12372)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12372
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_2855"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    7331..7357
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2472 a         3787 c         3824 g         2260 t
ORIGIN      
        1 actccagctc cgggccgacg ttcatggtgt accccatctc ctcggccttc ttcatcgccc
       61 tcttcaggac gtaccggggg tctccctcga agggctggcc gttgggcttg tagacgtcac
      121 atatcagccg cgccacgttc ctgttctccc tcgccctcca ggggaggatg gcgaaggagg
      181 atatgtccgg ccggaggagc atgtcggact cctnncctcg atggaggagc cgtcgaagcc
      241 ggtccccgag tcgagggcct ttccgagcct ggtggcgggg atcgagacgc tcttcacgag
      301 cccctggatg tcggtgaact gcagcctgag agactcgacg ttcctgtcgc ggatcgtctc
      361 taacaactga tctattgagt acatgattgg tcatacaccc cttgatgcgg gtctccaacg
      421 ccgccgccgc cgaccatccc cctccgggac cagaccgatg cggccgggcg gcggacgggg
      481 gcggcggcag attcctctaa agactccaaa tggaccgacc tcctttaata tagtcatcgg
      541 cccgatgggg ctcaattacc cttatctatt ggcgtatctc actccctcaa cggtagatca
      601 aatgtctggt tcaaaggttc tggtagttga cgcaggcggc aggggaaacg ccatcgccca
      661 tgccttcgcg aggagccccc tggtggagcg ggtctacgtc gcccccggga acgccggaag
      721 ctccatgatc gagaagtgcg agatcgccct ctcgaagggg cggcccctca aggagatcgc
      781 cgacctcgcc accttcgcca ggagagaggc ggtagacctc accttcgtcg gcccggaggg
      841 gtacctatcc gacgggatcg tcaacctctt caccgaggag gggctcgaag ccgtcggtcc
      901 gagacgggaa gcatcggtcc tggaggggag caagggctac gcgaaggacc tcctccggga
      961 tatcggggtc cccatccccc gccatcggag cttcggcgat ccggaggagg ctttagggta
     1021 cgtctcctcc ttctacgccg ataatccagg gatgaacctg gtggtgaagg ccgacggcct
     1081 cgccgccggg aaggggtcga taatctgcgg ctccgaggag gaggcggcct cggcggtcca
     1141 gaggatcatg gtggagcccc gcctcttcgg cgacgccgga aggcgggtcg tcctcgagga
     1201 gaggctagaa ggaagggagc tgatgttctt cgccgtcacc gacgggagga cggtcctccc
     1261 cctggagtcg gcgatggact acaagcgggc cttcgccccc ggagaggagg tggcgatccg
     1321 gctctacaac agactatcgg gagacccgaa cctcgaccac aaccccaaca ccggagggat
     1381 gggaggctac tccccccacc cctggctcga cggcgacctc gcggagcgga tcatggagga
     1441 gatcgccgtc cccaccatcc ggggccttaa agagaggtcg ggctttgagt accgggggat
     1501 gatctacttc ggcctgatgg tctcggagga ggagggcgaa agggtccccc gggtcctgga
     1561 gatcaacgtg aggctcggcg accccgaggc ggaggtgatc cttccccgcc tggagacgga
     1621 tatgtacctc ctctcccggg cggtcctgga tggggagctc gaatccctga agctcaaatg
     1681 gtcccccgac tattgcgtcg gggtctgcgc catcagcggt cgggtggtga agcccgccgc
     1741 cagcggggtc caggaggagc ggcccggcta ccccggcccc cactatacca acatgccgat
     1801 caggggtcta gataaggtcg atcccgacgt cctcgtatac cacaacggaa ccgccttcgg
     1861 gagggacgcc aggggggagg agaggctctt caccaccggc gggcgggtcc tcaccgtcgt
     1921 cggccggggc gccaccctcc cggcggcgag ggagagggcc tacgagaaca taagaaggat
     1981 caggttcaac gggatcaggt accggcggga catcggcctc accccctgaa ttcggggttt
     2041 ggagatgagc tcggcgggag ggatttcgaa gctgagggga tggtcgatag atgggagaag
     2101 gttcggataa gggatctgat cgaaggacgg tcgtcctggg gctggagggg acggcatgga
     2161 acctcagctg tgccctcgtc gacgaggagg acgtcatcgc cgaggagagt gccacctacg
     2221 tccctgccaa gggggggatc cacccccggg aggcggccca gcaccacgct gagcacatgg
     2281 cgccggtcgt cggcgaggtc ctggacgccg cccgacgcga cggcatcgcc atcgaagccg
     2341 tcgccttctc ccaggggccg gggctagggc cgtgcctccg gacggtggcg acggctgccc
     2401 gggccctcgc gctccgtttc aatgtgcccc tcgtcggcgt aaaccactgc atcgcccaca
     2461 tcgaggtggg caagtggaag acgggtgctg ccgatccggt cgtcctctac gtcagcggcg
     2521 gaaacagcca ggtcctggcc ctgaggcgcg gtcggtaccg gatcttcggc gagaccctgg
     2581 acatctccgt cggaaacgcc ctcgacaagt tcgcccggca ggtgggcctt ccccaccccg
     2641 gcggccccaa attggaggct ctggcgaagt cggcgaagga gtacatcccc ctcccctacg
     2701 tggtcaaggg gatggacttc tccttctccg gcctctcgac ggcggccgcc caggcggcga
     2761 agaagtacga cctcgccgac gtctgctcct ccttccagga gacggccttc gccatgctcg
     2821 tcgaggtgac cgagagggcc cttgcccacg ccgagaagga ggaggtcctc ctggtgggcg
     2881 gcgtcggcgc gaactcccgc ctccgggaga tgctcaatat aatgtgcgaa gagagggggg
     2941 cgcggttctt cgtccccgag atgaggttca tgggggacaa cggctccatg atcgcctaca
     3001 ccggccttgt tatgctgaag gcgggggtga cgacgcccct cgcggagtcg agggtccggc
     3061 cggggtatcg gacggacgag gtggaggtcg tgtggaagta atgtatatac cattccggca
     3121 attgtatcga tttgttaata tgtaacctta ttgtgcaaag ttaaaacctc taagttgatt
     3181 atgagaggat tataaattta tgtgtagaag agggtggacc ggactttgaa gaaggcgacc
     3241 atcgagagga agcttgcgat ggtcatcatc acgtccctgt tcttgacgag ggtcagcttg
     3301 gaggaccgcc tccagctacc cctgattcag ctggcttcag atccccgttg tcacggcgac
     3361 gacggcgttt ccgggatttc tgccggtggc aaaggcgttg ggctatcggc gtgttcacca
     3421 cgaccatctt cgtctttgca atgtcggcca tggggcagag ctttgagatc gtctggtgga
     3481 actccggccc acgctctcga agacgatctt cgcgcccatg gtcatgagga cgagcttgtc
     3541 ggagaagaag tactggatga gcatgaatat gctcacgaag gcgatcatcg ccgtcattcc
     3601 tgcgccctag cttgctagta tcgcgaaaag gccaggtaga cctgggccaa gagggacatt
     3661 gtgaagagca tcctcgtctc caatccccca tcgtgctgcc attttggttt catgatcttt
     3721 ttacaccttg agataagatt gatctgggga gttatatttt tcttctggga tttaaggggt
     3781 ttgttgtcga aggagacgaa atatcgaatg atcctgatcc gaggagccat caatgggcga
     3841 ctcaacagaa attcaagcag ctggagatgc gataagttta ttttggtctc aaagatactc
     3901 aaacgtgatg gcgaagttaa ggcgggggaa ggaaggcggt tggaaggaga acttttgggt
     3961 aggaatagtt gtcatcatca tcacggggct tttagtgttt ttcctgtctc aagcatatgc
     4021 agagcataga gaaaatgata gattacaaag gcaaatagat tcgtctcttc gtgaagccta
     4081 ttctcgactc gattccggcc agtacgagga agcgattaac ttatacgcag aaactctgga
     4141 taaaatatcg tcaaaagatt atccgtatga atttgctaat agccataata atatgggaat
     4201 tgcatattat aatttggctt tagcaaagga taaagaaaaa aatctaaata acgcattcca
     4261 agcttttgaa gaagctctca gaatattcac tatcaagcga taccctgtca attacgccag
     4321 gactcaaaac aacctcggag ctgcttacgt cactcttgct gaggtcagag acgcagagga
     4381 aaacatcaag aatgctatcc aagcttacga ggaagcccta ataattagga ctttagagca
     4441 gtacccggtc gattacgcaa ctactcaaaa caacctcggg aatgcttatt gggctcttgc
     4501 agaaatcagg gacaaagaag agaacttaaa taaagcgatt caatcgttca atgaagcctt
     4561 aaaaattaga actgtcgcgc agtgtccgtt ctattacgca gcaacccaga tcaacctcgg
     4621 gaattcctat caaagtcttg caggggtcag agatgcggag aagaacttaa aaagcgccat
     4681 cgaggcttac aaagaagctc tcaaaatata ttctatcgaa cgatacccga tcttatacgc
     4741 aacgactcag aataatcttg gaaatgttta ttgggctcta tctagggtca gaaatagtga
     4801 ggagaaccta atgagcgcca gcaaggctta caaagaagct cttaaagtta gaacggtaga
     4861 gcggtatccg cacgattatg caatgactca gaacaatctg ggaaatgctt attcggctct
     4921 ttatcgtgtc ggcgacgagg agaagaacag caagagtgcc atcgaagctt acaaagaagc
     4981 tcttagaata ttcacgcccg agcagtaccc gatctattac gcagctacga agagcaacct
     5041 cggaaatgcc tacataaatc ttgcagtggt cagagacgca gatgagaaca ttttgagtgc
     5101 tatccaagcc tacgaagatg ctttgataat taggactgta gaattgtacc cagttgatta
     5161 cgcgacgact cagaacaacc taggaaatgc ttatcttcat caggctcttg cagaaatcgg
     5221 agacaaagaa ggaaacttaa ataaagcgat tcaggcattc gaagaagctt tgaaaattag
     5281 aactcaagag ctatacccaa tcgaatacgc gcagactcag tacaacctcg gaaatactta
     5341 tcatgctctt gcagaaatca gggacaaaga ggagaactgc aagagcgcca tcgaagctta
     5401 cgaggaagcc ctgaaaactt ataccgagga agaatacccg ataccgaatc aaatcgtcaa
     5461 atcaaacctt gaacttgcgc aaagtcagct tcagcagtga ttcttaactt cgactctgta
     5521 cctaagatgg ggagggtcga ggccctcttc cagcccgacc ttactcctcc ccccgctttc
     5581 ctgtaaaaga ccctcttcgc cttcccctcc tggcggggca ggccgccgat ggggcggacg
     5641 tcccccttcg ggctgaagcc gaggccctcc ttcagggcct tctcgacgac gcggccggtc
     5701 acccggcctc ggcctcgacg ttgatccgga cggccttcat cccgtcgtcc tcctcgtgta
     5761 tgatcccgaa gctctccccg cccccggagc ctatgagcct ttcacccctc gattcaaata
     5821 acaaactata tcactccctt aaaacctatc cccgtcgatg atcttccaga tcctggacgc
     5881 caactacgtc tacgaccccg acgggtttcc catagtccag ctcttcggga cgaccccttc
     5941 gggcagaccc gtcaccgtcc aggcctctgg atttcgcccc tacttctacg cccgaatcga
     6001 cgagaagagg atcgactccg ccctcgaggg gatccgggcg atgggcctct ccgccgaggc
     6061 ggtggaccga ttccggcccg tcggctacca gaggcggccc gcaaagatgc tgaaggtcgt
     6121 cgccaaggac cccaagtccg tccgggagtt cagggagtcg gtcctctccg tccccggagt
     6181 cctggaggtc tacgagacgg acgtcctctt caagaaccgg ttcctcatcg acctcgacct
     6241 gggggggatg aggtgggcgg aggtggaggc cggcaatggt ggatcggatt tggagacggc
     6301 ggcggggtct gccaccatag gccccgtccc ctatacctcc tttcggcccg cggggcccgg
     6361 tccagcggtg gctggagagg cggagagggg aggagagggg gaaagagcag ccaatggaga
     6421 ggcggggagg gagaaggtcc ccggggcgga ggcaaggggt ggcgcgaagg cgatccccaa
     6481 cgcccccctc cgcaccctgg ccttcgacat cgaatgcctc cccctccacg gggcgatgcc
     6541 aagccccgag acctcgccgg tgatcatggt cagcctagcc ttcgacccgc cttacaaagg
     6601 gcgagaagac ctcgtcctcg tcggccggga tctggactgc ccccggggag acgtcctctg
     6661 ctgcggtggc gagagggaga tgctcgcccg gttcgtcgag atcctcaagg actacgaccc
     6721 cgacgtcgtc gccggctaca actcggcgga gttcgacttt ccgtacctgg aagagaggac
     6781 gaggaggctg aacctggagg tccgggtggg aagggacggc ggcccctggc gggtgagaaa
     6841 gatcgtcaac cagacccgtg tagagatcac cgggagggtc gtcgtcgatc ttctgcccct
     6901 ggtgaggtcg tccttcagcc tcaagaggta caccctcagg aacgcgagcc tggagcttct
     6961 cggggtcgag aagcacgacg tcgacccaaa ggagatggag gctctctggg ccgcctccgg
     7021 cgacgatttc gcccggctcg tcagctactc ccgccgcgac tcctacctcg ccctccacct
     7081 cctcaccgac ctgcagctcc tcgacaagta catcgccctc gcccagacga gcgggtcgct
     7141 cctccaggac gtcatcgacg gcggccagac cgggatggtg gagaacttcc tcttccgaaa
     7201 gttccaggag agggggaggg tcatcccccc caagcccgac gctgacctct cttctaccag
     7261 ggcggcccag agctccgagc tgaagggtgg agcggtcctg gagccgaaga aggggctggt
     7321 ggaggacgtt nnnnnnnnnn nnnnnnnnnn nnnnnnnatc atgatggccc acaacctctg
     7381 ctactcgacg gtcgtcaccg acgagcgccc cccatccggg ggggtcgtaa agccgccctc
     7441 ggggggggag ttcgtcctcc cggcggaggc gccggggatc gtcccagggg tcctctccga
     7501 cctcctggcc cagaggaccg atatcaagcg tcgtatgaag tcggcttcgg aggaggagcg
     7561 gagcatcctc gacgcccagc agtatgccct caagatcctc ctaaactcct tctacggcta
     7621 ctccggctac gcccgggcca ggctctacag cctccccctc gccaacgccg tgacgagctt
     7681 cggccgaacc aacatcctga acaccaagaa gatggtcgag gagatcgggt cggtctacgt
     7741 ccgggacggc aaagccctcc tccccgagga gatggcctcc gcttccggcg actccttccc
     7801 cgcagtcgga gagttcaagc gtttcgacct ctcggtcgtc tacggcgaca ctgacagcgt
     7861 attcatccgc ctcaagctct tcggcggcca ctcgggaaag ccgaccctgg aggaggcgga
     7921 gctggtggga aggaagatcg ccgagacggt tacgtcccgc cttccgccgc cgatggagct
     7981 ggtctttgag gccttcgccc gccgggggct cttcctcgtc aagaagaggt acgccctctg
     8041 ggtcttcgag aaggtcggcg gggagtggcg ggataagatc aaggtccggg ggatggagac
     8101 ggtaagaagg gactggtgcg agctgaccag caagaccctc aaccggtctc tcgagctcgt
     8161 cctcatggag gggagggtcg aggaggccgt cgacctcgca aagtcggtga tcgaccgcct
     8221 ccagaggctc gacctccgga aggacccgga gatcctcgag gacctggtcc tgacgaggag
     8281 gtacaccaag agcaccgcct cctacaagaa caagcagccc cacgtccagc tggccgagaa
     8341 gatgagggcg aggggcggcc ccgtccccca ggtcggcgac cgggtcccct tcgtcatcgt
     8401 ccgggggcgg gggctcttcg tcgaccgggc cgaggacccc tcctacgccc tccagaacgg
     8461 ccttttgatc gacaccgact actacatcaa taagcagatc ttgccgcctc tgctgcgcct
     8521 cctcgcgccc ttcggggtat caaaggagca gctcgtctgc tgccccgagc agcagcggct
     8581 cttcgacctc gcgccccccg gggcggcggt ggcgaaagga agggctccgt ccgggccaac
     8641 aggcgagggg caggagcaga agcagaagtc cctcttcgac ttctgatctg ctgccttcgt
     8701 tcccctcccc cattccggcc ccccttccca ccctccgcct ccgacctcag ccgttgatct
     8761 tctgcaatag ccaggccata ttcgagccga ggaccctcat cgtctccatc ccctcggcgt
     8821 ccgcctccac atcctccggc gcatagccga agccgaggtt ccagtagctc gaaccgggga
     8881 ctatcatctc actgatgaag aataggtggt tgatcgagtc gaaggcgtgg atcgccccgg
     8941 ccctgcggac ggcgactacc gccgccccca ccttcctccg gaagaggccg ccgttggctc
     9001 ttgcgacgaa gcctgcccgg tcgatgaggg ccttcatctc cgccgagacg tcggcgaagt
     9061 aggtcggcga ggcgaggatg atcccgtccg cctcgaccat ctttcttatg cattcgttga
     9121 cgacgtcgtc gtcgatggtg cagcgtctgt cctgattcgc gacgcagccg cggcaggcgg
     9181 tgcatccccg gatccgctct cccgcgagct ggaccatctc cgtctctata ccctcttttt
     9241 ccagctcttc caggaccctt cgcacgagga tggcggtgtt tccgtccctc ctcgcgcttc
     9301 cgttgaaggc gacgactttc atttttatct aactcctttt ataacttctg cagctcgcca
     9361 cgaacctctc caccgggaag gtggagaagt gggtgtgaag gtagcttccc agcgtctcat
     9421 gctcgacgag gccatcttgg tccccgacga tccctttacc ccgccggaac cggtaggcga
     9481 accgggagtc cctcgcgcag tccatcttcg agtagtggaa ctcgtgaccc aggatcgccg
     9541 atcctctcgc gacgacggga ttctcggcga cgacgtcccc ctcgacgtag ccgagggcct
     9601 ggagccttcc ggtcatggtg gtggatgcgg ggaggacccc ggccattttg ttctcagaat
     9661 cgccatcctt cgataccacc gattcgcaga ggtacatcag gccgccgcac tcgccgtaga
     9721 tcggcatccc gtccccggcg gcctttttta tctcatggcg ggtcggcgac tcctccaggg
     9781 cgctggcgta cagctcgggg tagccgccgc cgaggtagag gccgtcggct tcggggaggg
     9841 ggtcacggat cgggctgaag aagcggaggt cggctcctag cctccgcagg atctcgaagt
     9901 tctcggcgta gtagaagcag aaggcctcgt cgtatccgac ggcgatcctg atcctcccgc
     9961 cctcccggtc cggcaccgtc tccgtctccg cttcctgcgc cgcctgcgcc tcaggctccg
    10021 caacctcgca gccgagctcg aggatcccgt cgaggtatgc gttctcctcg aggaagtccg
    10081 cgagggcatc caggtcgtgg cggctctcaa agcccatcag gagccccagg tggcggctcg
    10141 gaagggctat ctcgccccgc cgggggaggg aggcgaggat cgggatcccc aggggcgcga
    10201 gggcctccgc caggaggatg cggtgccgtt cgcttccgac ccggttgagg acaagccccg
    10261 atatcttcac gccggggtcg tagctggagt agccgagggc catcgccgcg gcggacctgc
    10321 tcgtcccgtg gacgttcatc accaggagga cgggggctcc gagggctttc gcgacggagg
    10381 ccgaagacgc cacctccgtc gcgtcgaggc cgtcgtagag ccccatcacc ccctcgatga
    10441 cggcgacgtc cgcgccctcg acgccttccg cgaaagagcg gcggactccg tctcttccca
    10501 tcatgaaggt gtcgaggttt cgagagggcc taccgcagat ggcagcatga tgagatggat
    10561 cgatgaagtc cggccccacc ttgaaggggg cgacgacgag accccgcctc ctgagggccg
    10621 ccatcatccc caggacgacc gtcgtcttcc cgacgccgct ggaggtcccc gcgacgagga
    10681 cgcaaggtat catgatcctt ccatcggctc cggggctaat ttatcctttc gaaggcggct
    10741 cgcggatatc caggggcccc gaagggctta gagatgggct agcagggtgg agatcaagaa
    10801 aactctataa ccctccctcg tcatggaagg gatatgcgag tcggagtcgt cattcggggt
    10861 aagtacggcg agcgtctcat cgagacgata agagagagga ccgactttga ggtcgtctcc
    10921 gtcgagatcc cccaagccct gccggagttc atcgaggacc ccgaggagtt cctcgacggc
    10981 ctcgacctcg accccgagtt cttctcttca gagctgatca tcacctactc cctccacccc
    11041 gacgtcaccc cggagatagc cgtccgggcc gggaaggggg gcgcccaggc cgtcatcgtc
    11101 cccggcggga tcgggagggc gggatcggtc ccggagctgc agaagatcgc cgaccgttac
    11161 gggatctaca tcgaggtcga cgagatctgc tgcaccctcg acccctgcgg cgtcgaggcg
    11221 gtcgacgcct tcgccgagaa gctcggccgg ccccggctgg aggtggaggt ggaggacggg
    11281 aggatagctc gggccgaggt cctccggggc tccccctgcg gcagcacctg gcacgtggcg
    11341 agggatatcg tcggggtcac ggtcgaagac gccccgcccc gggccggcct cctctgccag
    11401 atgtacccct gccgggcggt ccggggcggc gaggggggga tccacacctc cggcgacctc
    11461 cacaaggccg ccatggagga ggctcttggg atcgaggggg ggctgaagat accggaccag
    11521 tcgaggccga taaagatccg ggggagggac cggccctgag gcgagaggat ctggtctctg
    11581 cgacggtggc ggtcctcgcc agggccgaga ccgtcctccc cctctgggtc gaggaggcga
    11641 taagagaggc ggccgcgaag gagaggagcg ccatcgccag atcccagctc tcggcgatcc
    11701 tcgagaacgt cgagatcgcg gccgcgggcg gcgtccccct ctgccaggat acgggcctcc
    11761 ccgtcttccg cctggagatc ggccggaacc tctcgatacc gcccttcctg gagaaggcga
    11821 tcgccgaggg ggtccgccgg gcgacggaag aggtacccct ccgccccaac gccgtcgacc
    11881 ccctgacgag aaagaacagc ggcgacaaca ccggcctggg gatgccggac ctgatcatag
    11941 acctcgtccc cggggactgc ttgcggatca ccgccttccc caagggagcg gggtcggaga
    12001 acggctcctt cctcgccatg ctcaaccccg ccgacgaccc cctcgatttc gtggcggtgg
    12061 aggtggcaaa gcgggcgggg agggcctgcg ccccagtctt cgtgggggtg gggatcggcg
    12121 ggaccttcga tctcgcagcg aggctcgcga aaaaggcgct actacggatg cccggcacct
    12181 cggagctcga tcggaaactc ttgacggaga tcaaccggat cgggaccggt cccatggggc
    12241 tcgggggcga cgccactgcc ctaggggtga ggatcgagac cgccttatgc cataccgcat
    12301 cgctcccggt ggcggtgaac cttcagtgct gggcgaacag gagcagttct gcgacggtga
    12361 gggatgacgg at
//
LOCUS       DASM01000020            5145 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_238331, whole genome shotgun sequence.
ACCESSION   DASM01000020 DASM01000000
VERSION     DASM01000020.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5145)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_238331"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1017 a         1667 c         1498 g          963 t
ORIGIN      
        1 cccccctccc cccccccctc cccccccccc ggacccccct caggtccagc acctcctcga
       61 ggcttctacg gatggtcttt ctcgggttga gggagccctc aggatcctga aatatcatct
      121 ggaccctccg gcggaaggcc ttccgctccc cgcccctcag atcccggagg tcccggcctc
      181 tatagaggac ccggccggcg gtgggggtct cgagcccggc tacgatccgg gcgagggtgg
      241 tcttcccgga gcccgacggc cccaccagcc ccagggtcct cccctccccg atctccaggg
      301 tcacatcctc caggacgacc ctcgtcctcc tccggacgat ccccctggag aaggcctttc
      361 cgatccccac ccccttcagc agcgttccca gcacctccac catctcctgc cgtcggacct
      421 caggggcggc ggccccgatc tgcagcccgg ctcagcccag gagcaccggg gatggaagag
      481 acagcccgag ggccggcgga ccgccgaggg ggaggagccg gggatgggat gaaacccctg
      541 caccgggagg gacttgagga gggcctgaga gtaagggtgg cggggccgct ccaggacgtc
      601 ggccaccggc cccgtctcca ccatctcccc ggcgtacatc accgccaatc gatcggctgt
      661 cctgcgggcc acctccaggt cgtgagttat caggagcatc gacgagcggt tcagatccct
      721 tatgatatcg atctcccggc agacctccca gaccagctcc tgatccagcc ccttcgtcgg
      781 ctcatcggcg atgatcagcc tgggccccgc cacccctgcg gaggctatca tcgcccgctg
      841 gttcatcccc ccggagagct ggaaggggta catccccccc tgacctgcga gccccagcct
      901 cgataggatc gaatccgcca tagaccgcgc cccggccggt gaggcccggc cgcctatgag
      961 ggccgattct gctacctgat ggcctatggt gtagacgggg ttaagggaga gggacggatt
     1021 ctggaatatc atcgagatct ccctcccccg gactctcgcc atctccctct cccccagccc
     1081 caggaggtcg cgccccccaa agaagatctc cccctttacc ttcgcatagc tggggaggag
     1141 ctgaagtacc gaggctgcaa ccaccgattt tccgcatcct gtctccccca ccagggccag
     1201 ggtctcctcc cttctgatat ccaggctcac atccacagcg gcatggaccg tcccagcctc
     1261 cgttggaaac ttcacagaga gcccgaggat ggagaggagc gtctcccctt ggagcttctc
     1321 accgggacgc gacacccctc tcctctcacc atcaccgttt acagccgaac tgctcgatgc
     1381 catctcttcc accctagatc tgttaatgcc tccagccaag atcagctcat ccccgtgaat
     1441 ccgtccaaga tgccggccac ccctccaaac agcatcctcc aaacagcatc tcaccccttc
     1501 attccggtat gcacctcttc cttttccagg tccatgctca tcaggaccag agagaggacg
     1561 aagagggtta tgcagatccc cggcgggagg taccaccacc agtagccgtt gatgaatcca
     1621 cctctggcga aggcgaagct gatcgtcgtc ccccagctcc gggctgtggg gtccacaagc
     1681 cccagaaagc ttatggacgc ctccgcgatc atcgccgagg cgacggtgag catgaacttg
     1741 gggaggatga cgtggacggt gttggggagg acgtccgtca ggattatgtg gcggtgggga
     1801 aagccgaggc accgggccgc ctccacaaag ctcatctccc tcacctggat cgcccgggac
     1861 ctgaccaccc gggccatgga gggccaccat aaaagcccca tcaagagggc gatcatccag
     1921 aagccggggc ggaggaaggc ggccagggtt attatcaggg ggatctgggg tatcatcagg
     1981 acgacatccg tcagccccat cagcacctcg tcggcccaac cccgatagta cccggcgacg
     2041 agccccacca tcgtccccag gagggtggcc agaagggctg ccccaaaccc gacggctatg
     2101 gagatcctgg acccgtggag gagctccgag aggaggtcgt tgcccatatc gttggttccc
     2161 agagggcggc tccaggaggg gggctggtaa ggttcgaacc gcgccctggg gtcggcggga
     2221 gagatcaccg gagcgaggat cgccgccgcc agaaatcccg taagcaacaa cacgccgatc
     2281 catctcatgg ggctggcgac cgccccggcc ctgccgaagg gtccgggaat taccatcctc
     2341 cccctccccc ggatctgacc ctggggtcta cgaccccgta tatcaggtcc gccaggatgt
     2401 tagcgatgat caccatcacc gttatgatca gcatggctcc ctggaggacg gggtagtccc
     2461 ggcagaggac cgcatcgtag atcaatagcc ccatcccgtt catggagaag actatctcta
     2521 tgaagagggc gccgctgaat atgaagccga agtcgagggc tatgagggtt atgatcggga
     2581 ggagggcgtt cttgaagatg gaggcgaaga ggatctcgtc ccgatagaga cccttggccc
     2641 tggcgtaaga gacgtagagg gacctcttct cctggatcac gctccccctc atgatcatga
     2701 agtttctgga ggcggcaaag agggtcatga tgacgacggg aagaaatagg tgatggagga
     2761 cgttcagggg gtctccggtg aggtagaccc ccttcagggg aaaccaaccc agcttaaagc
     2821 ccaggatata aaccgccacc agggatatga agtaaggggg ggagctgtag atggctatgg
     2881 cggccagggt ctgaagcctc cgggaccccc tcccctcgtc ccatccggcg agggccccga
     2941 ggtacgtccc caaaagggcc cccatgacga tggagggcaa gaccaagaac aaggtccacc
     3001 gcatcctgct ccaaagaagg tctgtcaccg gagagcggaa gtgataggaa tagccgaggt
     3061 ccagccgtcc gagggccttc atgtagccta tgtactgggt ggagaggggc tggtcgaggc
     3121 ccatcttctc ccgcatggct tcggcggcgg cccccgagaa gtatacgtcc tctcccagga
     3181 ggttcgtcat ggtgtcccca ggcatcatcc gcgggatggc gaagttgagg gagaggatca
     3241 ggaagagggc gagaaagtac ctggccagct tgaccctata gatcatgccc ttaatccctc
     3301 cctcagcatt tcaggatcca tttcgcagaa tctatttaac cgttagaggt gccggattgt
     3361 tccggacctg atggcccccc actctcccaa atcgtccatc tgggatgagg tggccttctt
     3421 tctccgagaa gtctgaggtt cgcagaaacg tcagagtcac cccagcctgt agaggagggc
     3481 gtcatcgctc ttgaccctga cccagggctc ttctccttcg gcgatcccgt ccatcgatga
     3541 ccccttctga ggggcggcga tcctcccccc atcctcaaga tagcgcatga tctcttccgg
     3601 gctcctggtg gatgaacaga gcccgtaaag gatgcaatgg aggggcaata cgtaatcccg
     3661 ggagaagatc tccacctgga aatcgtaacc catctcctcc aggatccgaa tcagacagga
     3721 gtaatccacc tgtagcctcg gaccataaac gaactcctgg agggggtcca tccttcctgc
     3781 cctgaagacc aggagcaccc ctctggagga tacatctatc atctttttta gaaaagagta
     3841 aacgtcctcc tcgaagatgc tgtaggcggc cagggaaaaa tccagatcaa ccagccgacg
     3901 gggatcgacg tccccccagc gggagttgat gatctcgatg ttgtttatcc ctttggagcg
     3961 ggccttctct tccagaaccc tcagctgatg ggcggaaggg tccagggcca gaacccttcg
     4021 catccttggt gccaggggta tggcgaaggc cccggtcccg gccccgacgt ccaggagaga
     4081 gcttccagag gggatgaggg aagagaccct ctccaggatc tcccccggat atccggaccg
     4141 ctccaaaacc tcctcataga cgggggcgta ctccctccag aactggtcgt tagtgatccc
     4201 ccgatcctgc agtatccgtt ggtagctggt ggattttaca tattccctcc agaggtccct
     4261 ccaccggctc atctcctcaa gccctctcca cgttcaggaa gttgtccagg ttgtatatgc
     4321 catagagggg gtctgaaacc catccctgcc agcgggagtt gtaaggcgtc tccaccttgt
     4381 tccagtagag ggcgatcccg ggtaggttct cggcgtagta gtcctgaacc tggtgagcaa
     4441 gctcttccag ctctccctcg gtggtggcgg cgaggatctc gtcgcagatc tggtggaaag
     4501 acggatcgtc cagaacgtgg aggaccccct ccccggtcct gcgggagtcg aagtacccgg
     4561 tgccccagtt ggcgtgcatc atcatccccc agggggtggt gcgggtgacg gtcagatcgt
     4621 actggtattc gtccttcatc gttatccaag tgctctggtc caccatcttc atctcggtct
     4681 tgatcccgat atcggcgagg tactcctgca atagctccgt tatccgctgg tagtcggccc
     4741 gggtcaggaa ggtgagatcg atcatctttc catctggggt ctccaggagg ctctccccat
     4801 cgacgtccct gtagccggcc tcctccagca atcttcgagc ctcctcggcg tcatagctga
     4861 gcttttccgt ctctttgaag tatcccatgc tggggggcac aaacccccgg ctggggacgg
     4921 atccgtagcc gagagcgtcc agcttaagaa tctcctggta gtctatagcg ctggcgagag
     4981 cctcccggaa ggcggggtcg gacatggggt cctctctaag gtcgaggccg aggaagatga
     5041 ggccggtgtt gtccgtctcc accacgtcga agcctccggc gctctcaagg gcggcgaggt
     5101 tggcgtaggg ataggtgccc gcatacttgt agtatgcatc cacat
//
LOCUS       DASM01000021            7822 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_152615, whole genome shotgun sequence.
ACCESSION   DASM01000021 DASM01000000
VERSION     DASM01000021.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_152615"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1600 a         2311 c         2349 g         1562 t
ORIGIN      
        1 cccctacaac ttcgaggggt tctactacga catcgatgac gacgtgggga cggagaggct
       61 gacggcccag ttcaccggag ataggatcgc cgacggagat ctgacttacg ggacctatcc
      121 ccagtcggtc cccttcgaat atgccgagtg ggggagctac aacgtcatag gcttcttggc
      181 cgagaagtac ttcgcagggt acaactccga gaccgtcttc accaacgagt tcagcctgat
      241 aaacgaaggg cagctgagaa aggtcctgat cgacgacgac cagtcctaca ccgtcaggag
      301 cggcaccacc ttccccctga aggaggggta tgagctgcgg ataaaggagg tggacctaga
      361 cggtaacaag atcttcctat ccatcaccaa ggacggtgaa gaggtggaca gcaaggtcgt
      421 cgagccgggg aggaccgtca ccggctccac cttcacctat gaagagcgga tcagcggcga
      481 gagggtgccg atcgtcgcag tccacgtcca gagcgtcttc cgggggaggg aggaggacct
      541 ggccaccatc gaggggatct tccagatctc cgacaccccc cgatccgtcg aggagggcga
      601 gacctacgac aagatggaga tcgatagcgt ctctgacagc ggcatagtga tgaagaacga
      661 cggctccatc agcctcggca gggggaggac cgtctccatc atggagaaca tgatgttcag
      721 gatcgccgac agcgaccaga ggctggtagt cccgatcgtc gaggtgaagg cggatctgaa
      781 gccgatgacc ctcgggatcc cgcaagctgt ggtcgggacc cccgccgaga tcagggcgac
      841 ctcggaggga gctccgatca tcggggccat catcaccatc gacggcgagg aggtcggaac
      901 caccaccgct gatgggatct acctctatac cccgccggcg gccggaagct tcgagatttc
      961 ggcgaagagg gacgagtaca ccgaggtgaa gggggtcctc gtcgttagcg aggtcgcgga
     1021 ggagaggatc ctggccgtca ccgcgcctca agaggtgatg aagggcgaga gctttgtgat
     1081 caaggccacc attggagtcg acctgaggcc ggtggagggc gcggatatcg gattcgagaa
     1141 cgtcaccatc gggacgaccg actccctcgg gaccttctcc tacgcctccg attccgtcgg
     1201 atcttacacc atcaccgcct ccaaggaggg ttatgagagc ggctcgagga ggatcgtcgt
     1261 cgcatccccc atcagaataa cggggatcga gatgcctgag acggtccggg ccggaaagac
     1321 ggtcgagatc accgccaccg ctgagaaccc gggggctgtc agcgacacca ccacgttgga
     1381 gcttagggtg aacggggtcc ccgaagggac gatggagttg accgtcgccc ccggcgagac
     1441 gggatctgcg gccttcgagt accagcctct ggagccgggg ctctacaccg tggaggtgag
     1501 ggggatccag aggacgttga cggtggagga gaagagctcc gccgggacca tcgtcgggat
     1561 cctggtgatc ctcttcgccc tgggtggagg agcttacctc tactccaccg gaaggctgaa
     1621 cgagatgctg gggcagatca agaaccgctg atcctgcccc aaaccatttt tttccgctca
     1681 ccatccgaac cggaggtctc tttgggtggg gtggaggtgg agggttccca gggaatgatt
     1741 gatatacccc tggctggctc tctcacggca gagtgtagca tatcggtttc ggccgactaa
     1801 aatttcagga cagtattccc atgaacttct ggcaaccgaa gatcgagacg atgaagaggt
     1861 cagatctgga ggatctgcag ctgagacgtc tgaaggagac ggtgaagagg gcctacgatc
     1921 gcgtcccctt ctaccaccag aggatgaagg atctgggggt gacgccctcg gatgtcgcct
     1981 ccctagcgga tgtgaggatc ctcccggaga cgaggaaggg ggacctccgg gagaactacc
     2041 ccttcggcct ctttgcggtc ccgagggaag aggtggtccg ggtccacgcc tcctccggga
     2101 cgaccggcaa gccgacggtc gtcggctata cctcccggga catcgagacc tggtcggata
     2161 tggtggcgcg ggacctggtg atggtcggct gcaccaagga agatgtattc cagaacgccg
     2221 tcaactacgg cttcttcacc ggggggctcg ggatccacta cggtatcgag cggatggggg
     2281 cgatggcggt ccccagcggc accgggagca ccaggcggca gatcgagatc atgatcgact
     2341 tcggcgtcac cgccctccac tgcaccccct cctacgccct ctacctcgcc gagacggtga
     2401 cggatatggg cctcgccgag gacctctccc tgagggtggg gtgcttcggg gccgagccct
     2461 ggtcggacag ggcccggacg gagctggagg agatgctgaa tataaaggcc tacgactcct
     2521 acggcctgtc ggagatgttc ggccccgggg tcgccttcga gtgccaggag cagaacggcc
     2581 tccacatctg ggaggaccac ttcctggtgg agatcctcga tcccgacggc ctcccctgcg
     2641 cccccggcga gcggggggag ctggtcctca cctccctcac caaggaggcg atgcccctca
     2701 tcaggtacca taccggggac gtcaccctca tgatggagga ggagtgcccc tgcgggagga
     2761 cgagcgtcaa gctccaccgg ttcctggggc gggccgacga catgttggtc gtaagaggga
     2821 tcaacgtctt ccccagccag atcgaggacg tcctcctcca gatcccggag atcggggact
     2881 acttccaggt cgtcgtcgac aggaagaggc acggcctcga cgagatctcg atcaaggtgg
     2941 agatgagcga tcgggccttc accggggagc tcgcggacct tgcgcggctc cagaagaagg
     3001 tggaggacga attgaagtcg gtgatgaaga tcaggtcgaa gatcgagctg gtggagaagg
     3061 ggagcctccc ccggaccgaa ggaaagtcga agaagatcgt ggacctccgg gatctttagc
     3121 gccggcgacg ggggcggagg tcccgtcccc ctctacggcc ggaggtcccc cttttctttc
     3181 gccatctcgg ccaccctttc ggcgagcctc tccaacaccc ccttcatcct ctcggagttc
     3241 tcgcagtggg agccggtctt ggggttgcag accgagaggg cgtccatcgc ccggtcggcg
     3301 agccgatcct cgcccatctc ctcgaggctc atcagaagct ccgccatact ccggttgaaa
     3361 tcccggcact tctcggcgtc gcagatggcg tcgccgtgga ggcggcggag ggtctcgacc
     3421 tctttaagga gggattccca gcggacccga tctctcgggt aaccatctat ggggggcggc
     3481 ggatcgacca taaccccttc ccacccccat cagagccttc agccatcggg taagaggccg
     3541 atatctcacc taaggagggg caatgctgcc gaagatctga tcggagccgg tgggggagtc
     3601 gacggaattt ctggaggctt cctctccgcc tcatcccagg cccctcgccc ggtcgaaggc
     3661 ctccttcgcc gcctcgtcct cccccagcat ctggtgggcc cagctcttgc tgctccaggc
     3721 ggagaggagg ccgggatcga tctccagggc ccggtcgaag cactcgaggg cctcggagta
     3781 gcggccgagc ctgttcaggg tcgatccctt ccggttccag gcctcggcga actccgggtc
     3841 gatcctgagg gcctcattgt agcacgccat agcatcttca aaccggagga gcatctggag
     3901 ggcgttcccc ttccggcacc accctgccgc cgaggcggga tcgatctcca gggccctctc
     3961 gtagctcctg agggccttgt cgtacctgcc ggtcatcatc agcccgtccc ccttcccggc
     4021 ccaggcggcg gcgttggcgg gatcgatctc cagggccctt tcgtagcagg ttatcgcctc
     4081 ggggtacctg ccggagccga ggagggagtc gccccatctc gtccagtccc cggccgtata
     4141 caaagtgacc tccggaggct catcgaccgg ctcttcggcg gggggctccc cggcggactc
     4201 gttcgtctcc tcctcgcccc gatcggcgtc gacgatccca tccccggcgg cggggccgaa
     4261 gggggcgata ggggcctcct cctcctcctc ttccttaacc tcagcctcct ttgcctcccc
     4321 ctcctgggag ccgctccggc tctggctcag ctcgatgatg ctgatctggt gcctcttcct
     4381 ggattcgggg gctgccgggg cttcaacggt gagattcgat ggaggtctct ctacagtaga
     4441 ccggttcatc ggatcgatct cggtctggtt tagtcctcca tcccccgggg cgccgccgtc
     4501 cccggagggg gcaggatctg gcatcaaggc cagatcctgg ctttcagcct cctccccggc
     4561 catcgcctcc gttgccggcc ccgattctgc ggaatcctcc gcttccgccg ggggctcaat
     4621 ggcggcctcc tcagggccgg aaggagaacg gtcgacgctg gacaatccgc ccacctcgta
     4681 gaagggccgg gacccgtcgt aatcgtcgta gaagttcccc tcccagaggt tcgtccccag
     4741 gtcgaaggcg ttgcggttct cgttctcctg gaggacgttc ttcctgacga ggttgccgct
     4801 cgacccggcg ttgatccata tcccgtactt gttattccag atgacgttct cctcgatgac
     4861 gcacccttcc gccccgtcga ggtatatgcc gtaccaccgg ttgtcggtga tcaggtttcc
     4921 ccggatggcg ttctccttcg acctcacctc aatcccggcc cagccgaagc ctccgttttc
     4981 gaggacgaag ccctcgatag tggcgccgtc ggcgaggagg gtgacggcgc tcccccctct
     5041 ggaggcgtcg acgatcgggc ggtcggtgcc gtccccggcg tcgcggaggg cgatgggctt
     5101 gtccaccacc agggtctcgc gataggtgcc gttctggacg gagacgacgt ccccggggcg
     5161 cgcggcgtcg atggcctcct ggatgctgga gtggtcgcag ccctcagggc cgacggtgat
     5221 ggtggccgcc tccgccgggg ggagggcgag gatcgccatc atcaagacta atatggatag
     5281 ggtggtcttc gaggtcatgg agcctcccgc ctggaggtcc tttgtacctc gatctaagaa
     5341 aatcttttgg tgagggccgg ggtttttgtc tcatgaaggc agacaacgta atcgccgcaa
     5401 tttatgaaac gggggtttcg ctccccctcc accatggtcc tggggagggg tcttcgactc
     5461 ggaggtccct ccggaggggc ccctggatcc gggttggtgc gaggaggctc gtatcccgtc
     5521 gcatatataa taaaaaagga aattatattt ataaatgata aaaatactca gctctatgga
     5581 tatcgactcc ttcccgcgga tactatgaat cttttattta taaaaacccc tgaaaatccg
     5641 agattttaat caaatctaaa aatactacat atcgccgtcg ggattcgacg acccggatcg
     5701 ggacttaaat ccagtaataa ccgcaatttc tggatagtat gggcgattct tcctctgaat
     5761 tgaaagtcaa atcaactgaa ttctgtttta ttgattccta tttgataatt atttatctct
     5821 ggaggtccaa aacgttatct gcgagtcaat tttgtcatag aagggaatta atctcctttt
     5881 agagaaaagt acatatatca agaaattatt gtttatgtcc atataatcta gggctctgga
     5941 gggagcgatc acccatctta tgactcgagt gcaacctata atcggggctg gagcatcatc
     6001 gagagtgctt gtcgatggat ataaccccca tagagccttt accgcttcaa aacacaattt
     6061 tatatattcg ggatgggaaa aatatgaata tatataggcc tatattatta ctatctcttt
     6121 tggcagcgac cctattgcca tcggtggcag aggtggatac gataaccttc tgcgactcgc
     6181 tgcccgccaa ggaagggggc tggtcagacc gccacatcct ccctctcttc gatccgacca
     6241 tgggcgagct cctccgggtc gatctctccg tggacctggt ggtaacgcag gattttcaat
     6301 ttgagaacga gggtcctgcc ggcgcgaccg tagacgtcga ctcggttttt gagctatcta
     6361 tcaccatgcc cgactccagc aacatcaccg ccgccgcctc cagctccatc agcgaggacc
     6421 tggctggctt cgacggcgag gtggacttct cgggcccctc ggggaggacg atcaaagggc
     6481 tcgcctccac cggctcagtt gcggaagagt acctggagct ggccgccttc gtcgccacca
     6541 tcccggacga gacgatcaac ctcccagcga gggctttagc caatagcaag acccagctgt
     6601 caggaaatac cgtatccagg atctccagcc ttgtggggtc aaaggtctgc gtcacttata
     6661 cttatgatgc agatgctgga ggtgaaggac gatgagacct atgagaaata tcctcctctt
     6721 cgccacctta gctctgatct tggcccccgc cgccctggcc acggagagca tcacgtattg
     6781 ccagcagttg acgcccttga atacgaattg ggatcggcag agcgtccttc cgaagttcga
     6841 cccgagcctt ggaactctgg tcaaagttga attgaacggt acgctgaact ggacccagat
     6901 tgtgaggttt gagcacgtcg tttctgagag tgatgccaac atcacaatcg atcaatgggt
     6961 atatttcgag ttgaccatgc atgacgatac tctcctcacc aatgagtggg agaactatag
     7021 cgaatggtat gtaactgttt atgatcaggt actagattat ggcggcactt cagggttcaa
     7081 ctttacaaat gcaaatctca cgtctacctc gaggaagaac tacaccgatt gcgatgatat
     7141 cagcgagtac atcgccaggt tcccggggga gaccaagaac atcaggacta gggccagggc
     7201 tactgtaggt gctgacggct ctggaaacta cgtactctcc cccacaacat cggccagctc
     7261 catagtctgc gtcacctact actatgaccc tccgacctgc atcgagggct acaagctcga
     7321 cgcggcgaac aaccagcctc tggagggctg gaacatcacc ctccaagact gtgacgggaa
     7381 ggtcatcggg tgggatataa ccaacgagac cggcttctgg aagatctgcg gccttcagtg
     7441 ctgcaacttc tccgtctgcg agatattgaa gcctggatgg atcgccgtcg atccggcatc
     7501 cggatgctat ttagatctga actcttcgga ggggaacctc acaggctaca acttcacgaa
     7561 caagaaccta tcctgcatct cgggctacaa ggtgatcaac tgcgaggacg tggacctatc
     7621 gggctggaat atcaccctct acaacgagac cgatgtcctc atcgatacca ctacgacgaa
     7681 cgagacaggc tactaccagt tctgtggcct cgatcacggg aactacaaag tctgcgagga
     7741 ggtgaaagat ggttggcaga atctgaccga tatctgcata gatgtggtcc tcggatacga
     7801 taacgccacc gataagaact tc
//
LOCUS       DASM01000022            5274 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_31482, whole genome shotgun sequence.
ACCESSION   DASM01000022 DASM01000000
VERSION     DASM01000022.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_31482"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1096 a         1614 c         1582 g          982 t
ORIGIN      
        1 tctgcggcgg ctgcgtcgac gcctgcccgg agggggctat ccgctacgct ttgtgggaac
       61 ggggcaattc acgaagggaa agatgaaaac cgcattatcg ctgaagatcc ctccctccga
      121 agatcttcga tggatctcat cgatggaacc acctgaggag acgaatcgtc gtcaccggtt
      181 ccccacccgg gatcgacaat tattcgtaat actcggaggg ttatcttcat aaaccacgga
      241 cggatgacag taaggcaggt gcgaggcctt catcaccgag aggacggaga gacaagctcg
      301 ttttggagga ggttgaaaga acgtgagcta cggcctggat tccggagacg tccgagaggc
      361 agaggacccc ttcaccatac tgctcgtaga ggacaacaat gcccatgcga tgcttatcat
      421 gagggccttt gaaaggctcg gcttcaccgg gaggatagag tggctgaggg atgggaaggc
      481 cgccctggat tacctccgcc tccacgaagg gggggggagg aacctcccga ggatggtcct
      541 cctggacctc cggctcccta aggtcgacgg ccacgaggtc ctatctcaga tcaagagttc
      601 ggagaggctg agggcgatcc ccgtcgtcgt cctcaccacc tccaccagcg acgacgacct
      661 ccggcaggcc tactccaacc acgtcaacag ctacctcatc aagcccctca gcttcgagga
      721 cctgaagcgg accgtagagg agatcaaaga gtactggctt ggctggaacc gctcccccag
      781 acccgcgtga aaaaaccctg aggcgagcga aaccgaggcc ttcacccatg acgctgcgcc
      841 gaagcatcct ggccttcacc ggggggatga tcctcatcct cgccctcatc tgcagcacct
      901 tcatgtgcca catgatgatg gggaccctct cggatctgga ggaggcctac gtccatcaga
      961 aggtcgatgg caccctcttc tacctccagg aggacctatc atccctcaag agggcggcag
     1021 aggactgggg gcactggaac gatacccggg acttcgtcat cggcgagaac gatcagtaca
     1081 tccaggataa cctgaacagc tggaccctat ccggcctcga cgtcgacttc atgatctact
     1141 acgaccgctc cggcaaccta ttctactcca gggccttcaa ccacgccacg ggagaggagg
     1201 tcccagcccc cggccggctt ctgagcctcg cgaaggacga ccccctgata gctcattcct
     1261 cgccggagga cggccttacg gggatcgtct ccgatccgga ggggcttctt ctggtcggct
     1321 ccaccacgat cctcgacagc cactggaagg ggccgatatc ggggaccttc atcgtcggca
     1381 ggcgcctgga cggggcgagg gcggaagggc ttgcgaggct gagcggcatc gacctgaaga
     1441 tggaggccac cgccccggtg ggggggtcag aggataccaa ctctaaaacc tctcttctca
     1501 tcgttaagga ggactcagat accctcatcg ccaccaagac gatcgatgac gtatacggta
     1561 accccaccgt cctcctggag gcccaggtcc cgagggagat ccgctcgagg gggctggaga
     1621 cgatccgccg gcaggtggtg gggatcttcc tcgcaagcct cctcttcggg gggctgatcc
     1681 tcctcttcct ggagctctcc atcctgacgc ccctggccac catcacctcc tccgtcgagg
     1741 cgatccgggg gcaggagaaa ggccaagggt cgcggatccc aacggtgggg ccgacggagc
     1801 tggccaccct cgcagagtcg atcaacgata tgctcgaccg actcgagggg tacaaccaga
     1861 agctcgccat gagcgagaag aggttcagga cgatcgtgga taccgcccag gactgcatct
     1921 tcatcaaaga ccccgagagc aggtacgtcc tcgtcaaccc cgtcatggag aggatatttc
     1981 agttgccagc ctccaagatg ctgggagaga gggacgaggt cttcttcagc gccgagacgg
     2041 cggctcggat ccgggagaaa gacgccgggg ttctctccgg agaaccgttc gtcggcgagg
     2101 tctccgccca tacccgagcc ggatcttcga tgaccttcca cgtcgtcaag gtccccctga
     2161 gggacgaccg gggccaggtc acagggatat gcgggatcgc aagagacatc accgatatca
     2221 aggaggctgg ggtcgaactc ctgaaaaagg acaggctcct ctccgcctcc gccgccgcct
     2281 cctactccct cctcgtcaac tacgatatcg accaaatcat catcgacgtc ctccagctcc
     2341 tgggggaggc ggtggaggcg gaccgggcct acatcttcga gaaccagacc gtagatgggg
     2401 aggtcttcat gagccagcgc tacgagtgga cgaaaggaga ggtcgagccc cagataaata
     2461 accccgtcct tcagagcctc ccgtactacc ccgattcatc cactttttat gagattatat
     2521 cgaggggaag accttatgta ggcctcatca aggaccttcc cgaatcggag agggcttacc
     2581 ttgagcccca gggtatcgtc tccatcctga tcgtcccgat cttcgtcgag gatcggctat
     2641 ggggtttcat cggcttcgac gactgccacc gggagaggtt ctggagcaac ggcgagatat
     2701 cggtcctcca ggtggcggcg gggtccatag gcggcgcttt catccggtcc cggacgaggg
     2761 cggacctgat cagggcgaag ggggagctcc tggagaggat cggcgaggtg gaggcgaaga
     2821 acgccgagat ggagcggttc gtctacaccg tctcccacga ccttcggtcc ccccttgtca
     2881 ccatccaggg gttcgtcggc ttcctccggg aggacctctc ggccttggac ggagataaga
     2941 tcaagaccga ccttgcgatg atcgaggagg cggtcctcaa gatggaccac ctcctcaagg
     3001 acaccctgag gctctcccgg atcggccggg tcgtcaaccc tccggaggat gtctccttcg
     3061 gagagatcgt ccatgaggcc ctcagccagg cctccgggga gctgagatcg aggggaatca
     3121 aggtcaccct cgccgaggcc gagggctggc ccagggtgaa ggtggacagg ctgagggtcc
     3181 aggaggctct gacgaacctc ctcgacaaca gcatcaagta catgagcgac aggccccatc
     3241 ccgagataaa ggtcggatgg cggcctgggg aagaggagac cgtcttcttc gtctgggaca
     3301 acggtatcgg gatggacccc gacctgcggg ataaggtctt cggcctcttc tacaagatcg
     3361 accccgactc cgaggggagc ggcgtcggcc tcgccatagt caggagaata atagaggtcc
     3421 acggcgggag gatctggatc gaatcggagg aggggagagg gacctgcgtc ctcttcaccc
     3481 tcccgacccc ctgaggccag atcaaacctt gagggggagg ccctgcccct cctgggggct
     3541 cgggtggcct tccccgccga cggcccgcct cttcagctcc cgccacctga ggagggcctc
     3601 ttctgcgatc tcgggaagcc gctggatgct atcggcctcc ttgacgacgt agtccatcac
     3661 cccggacttg aaggcctgaa ctgctatctt ctcggagccg aatccagtga ggattatcag
     3721 ggggaagccc acctccaggg gacgctccgc cttccccgcg aggtcgaggc cgcagccgtc
     3781 cgggaggaga tagtccgcca ccaccaggaa cggtcggcca tagctatcct cgatccacct
     3841 cagggcatcc cggagctggc tggcgtgatg gaccctccat ctcgggctgc tctcctcgaa
     3901 gatccggcgg atgagggtgg cgtggccggg gtcgtcctcg acgatcatca cgtcgaaggc
     3961 tccgggttct tcgccggcca gagcctgcgc ctccgcctcc gggtggacga ccgatcggaa
     4021 taccctcttg aggtccccct catccagctc ccgagatctc tgagcgtagt attcgaagag
     4081 ctccatcccc caggcgacgg cagactctcc gagatagatc gcccccgtcc ccacgtcgta
     4141 gactccgtcg tggcgataga gccccagata gaggaaggat tcggtgacga agagggcgag
     4201 cctgagatct tcaccgatga gccggagcct gaaactttcg aaattgagga gcctctccac
     4261 ctcgtaggcg gagaggctcc cgtcgtcgag gaggcttctt gcgatcttct ccgtcatcaa
     4321 aagatccacc cgggaccccc tccggagggc gttcgtgaag gccttcgagt ccttgggaaa
     4381 tatcaataga gggaggatcg cggaaatctt cttcgaggtg gagagcagag acaccagcct
     4441 ctcgtggatc tccagggggg aggcggggtt tgtggagacc atctccgatc ggcggagcat
     4501 gctcagcctt tcgagcatct ccggagggat ggagctgata tcgtggttca gccagaaatc
     4561 aggacgggcc tcgatatcat ccaggaccgc catcaggctc tcaaggacgc agactcggat
     4621 cttccccagg ttcgtcagcc tatatccctg ccgacccctg gagatcagat cgtcatcggt
     4681 catgtccctt atggcatgga gaattgtggt atttcttcct cccaggaccg cctggaggtc
     4741 gtttacatcc atctctctat attttaaaca gagcaagatg ttcagcttca gctcagagct
     4801 ggcgaatacc ttcacccgtc tgaccatatc tctttcgtcc atgttgaggt ccccctcatt
     4861 ttaagggcct aaatggcgta gatccacgaa tacccgggag agattctcga tccgacggaa
     4921 caactcccca gttatcggct tgatatcagc ctccaccgtc caatttcgcg agcccatctc
     4981 ctcggcttgt gttttttcct tgatttcgcc atataagtag cttacgacga tccagatgct
     5041 gtgagaagct ctgaaggtct catcgtaccg aaaagaaggc ggatatcggg ggccaggccg
     5101 caaccccgga tgccgccggc ggcagggaag aaagccccga tcgatcccga tatggtggat
     5161 ctaaactgcc tccgccggcc gggggggagc cgtgagaccc tgccaacctc cttttcgata
     5221 tgggaaacgt atatcaggaa gtttgggaat agatccggca ggtgttctga cttg
//
LOCUS       DASM01000023            5312 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_38349, whole genome shotgun sequence.
ACCESSION   DASM01000023 DASM01000000
VERSION     DASM01000023.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_38349"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          982 a         1700 c         1718 g          910 t
ORIGIN      
        1 gatctcctgg gccatctcca ggagcctacc gggatttcgg gcgtcggaga cctcaacctt
       61 tgaggccgac ctcaccatcg aggaccggag ggcgacgatc tcctcgacgc ccttcagagc
      121 cagctcgcct ggccgggaga agtggagggg atcgagggcg aggccagggc ttgagcctga
      181 gccgaggttg agcccaccgc tctcgtccgc cgggaggagg gggccggcgg agaccctcgg
      241 gtagccgaac ctccccacga agacctccgg gggcgaaggc ccctcgaggg tcgtcccgag
      301 cttcgggagc tccgatgcca cccggaagct cctcaggatg gggcagaccg gccttccgca
      361 gagccnnacg cacctcgtag ccaacggatc gaaaacggct tttataggat atagccccta
      421 tcatcgaggc atgatggaga tcaagggcga cctcgtcccg gcgatcgtcc aggacgccga
      481 aagcggcgag gtcctcatgc tcgcctacat gaaccgggag gccctggcga agaccctgga
      541 gacgggaaaa gcccactact ggtcgaggtc gaggggtaag ctctggctga agggggagtc
      601 ctccggccac ttccagaagg tcgttgaggc gaggatagac tgcgatctgg acgcgatcct
      661 cctcaaggtc cgccaggagg ggggggcgtg ccataccggc tatcgatcct gcttctaccg
      721 cgacctggag gggaagatcg tcggagaacg ggtcttcgac cccgaagagg tctaccggtg
      781 agggagaggt cgcgggaagg ggcggagggg ggggcggccg ggcggtacaa cctcgccaga
      841 agccccctcc tccgggtcga ggcgtcggtg gaggagggga gggtccggct gaggggcaag
      901 ggtccgctat cccgcctccc cttcgtgcgg tccgccctgg ggatcgccga cggccagatc
      961 ccggtctcgg ccggggagag gctgatcctc tccacctggt ctccgccgat tccgagccga
     1021 gccttcgacc gcctggtgaa gagctacctc aagtcgtctt ttgggatcag gacccccgac
     1081 caggtgacca tatccatcac cgaagagtgc cccaaccgct gcctccactg cgccctcccc
     1141 gactcaggca gacaccaccg tctggagccg ggcgaagtcg aggacgtcgt cggccaggtc
     1201 ctggatttgg gaacgaccct ggtcatcttc gacggcgggg agccgacgac ctaccgggag
     1261 ctccccgacc tggtccgggc cgtagacgac cgggccgtca ccacgatctt cacctcgggg
     1321 gcgggtttca cccccaagct cgcccgggag ctgaaggatg cggggctctt tgccgtcaac
     1381 gtcagcctcg actccgccgc cgaggcggag cacgaccgga tgaggggtag ggcaggggcg
     1441 tttcgcgacg ccatgagggc cgtcgagaac gccctcgccg ccggcctcct ggtggaccta
     1501 tacgccgtcc tccggcggga taacgtctcc gagatcgggg cgtttcatcg cctcgccacc
     1561 gagatcggcg ctgatgagct gaccttcttc gaggtcgtcc cgacggggag gtgggcagag
     1621 gaggtcggtt cgatcctgtc ggtggaggac tacgctgccc tcgaccagtt cgtctcggag
     1681 gctccgccgc cccggacctt ctccgtcccc gccgccttcc gggaagtggg ctgcttcgcc
     1741 gggaggagct ggatgcacat caccccggag ggggacgtct atccttgcgc ctgcctgccc
     1801 cgggtcgttg gaaacgtccg gagggattcg gtaaagagaa tctggataaa gatggcgaag
     1861 ttgccgtatc gggggaggaa aatctgtcct gcgcgcagtt ttcggcagaa agatcaacac
     1921 gtatttatag ccgataatcg tgagagtcag cccgatcaga atggaactga cacagataca
     1981 gcgggagata ctgactgcgc tgataaactt acaacgccgg gagggaaggg ccgttaaggg
     2041 ggaggagatc gcctcgatca tcgaccggaa ccccggcacc ataagaaacc agatgcaatc
     2101 cctcaaggcc ctccacctgg tggagggggt ccccggcccc aagggaggct accgagccat
     2161 ggccgcggct tacgacgcca taaacctgga cgccgacgac gacgtggtgg acgtccccgt
     2221 cgtcaggaac ggatcgcccg tggagggggc caccgccaac gagatcacct tctacaccgt
     2281 catgaggccc aaccagtgta gcgggatcgt ccagatcatc gggaacatcc gggacttcaa
     2341 catcgacgac gagatcgaga tagggccgac tcctgtcaac aagacccaca tcaagggggt
     2401 ggtcaccggg agggacgaca ccaccaaccg cctcatattc gacataaagg agatggcatc
     2461 gatccccaag atcccggtga ggaacctggc gaggcccgcc atcaccatcg acccccagat
     2521 ctccatcatc gaggcgtgcc agaagctctc cgactcgaag gtccgggccg ccctcgtcgg
     2581 gaagaacggc gactccaggg ggctcatctc cctggaggat ctcgtccgag gagtggcagc
     2641 cgggaagaaa gatcatccgg tggaggaggc gatggagagg gagtacgcct ccgtcgaggg
     2701 cgatgagccg ctccacgtcg ccatcagcaa gctcggaaag accggggcgt gccaggtcgt
     2761 cgtcaccgaa tccgacgtcc ccttcgggat gctggaggca actgacctcg tccgctgcat
     2821 ggtccggttc tgagccccag cccgtgcggc tcaggagatc tccgtccccg agtggccgcc
     2881 ctcggcccct cttctcagat tttcgacctc gccgccgtag acgacggcgg cggggatccg
     2941 gctccgctcc aggatcttcg ccgccagggg gtccaccggg gaccgcgacc cggccttcat
     3001 ctccgtctcc atggcgaggc ggacgaggtc tttggcggtc atcttatcga acttcttcgc
     3061 ccccggatcg atctcggggt cggcggaata gacgccgtcc acggaggtgg caacgatcag
     3121 ccggtcggcg ccgacgtact cggccaagag ggccgccacg gcgtccgtcg tctggccggg
     3181 agccatcccc ccgaggacga cgatctttcc tccggcagag gcagaaaggg cgccagcgta
     3241 gctctcggct ggctcggggg cggcggctct cccgagggcc gcgatcagga gcatggcgtt
     3301 catcttcgtc gccccgatcc ccaaaaggtc gcagaaggcc tcgctcgcct gaagggcccg
     3361 ggcctttccg atgtactctc gggcgatctt gcccccgccc accaccacga agatctggtg
     3421 ctcttcggcg aggctcttta gagccgcagc ataccccttg aggctctcag catcgcggcc
     3481 cgccaggatg gagccgccga gggaatagac gagtatcatg tgaggagggc aagattatga
     3541 gcgaataaaa aggttccttg tgatctagcc ggaccactcg gccagagctt tgcggacggt
     3601 ctcggcgtga gggctctcga tctcctcgaa gatggcgagg gcggatttgg ccagggagat
     3661 cgcctcctca ttttggccca gggcgcgaag agagaggctc atattgaaga ggtgggctcc
     3721 ttcttaaaat cgaaagataa gttggtgaat gaagatgata agtttttgca taacaccaaa
     3781 caatgagaaa aaaatggttg gaaagaggcg ctctaaatct tatccagcgc caccgcgacc
     3841 ttcccgaaga tctccttgat atccccgacg ccgatgaccg tggccacgat cccccttttg
     3901 tcgtagtagt cgatgagggg ctgggtctgg gcgtggtagg cctggagcct ctccttgacc
     3961 gtctcctcgg tgtcgtccgc cctctggtag aggtcggccc cgcaggcgtc gcacttgcct
     4021 gccacctttg gcgggttgaa ctgaacgtga taggtggtcc cgcacttgca catcctccgg
     4081 ccggtgaccc tcttgaccag ctccgcgtcg gggacgtcga tgttcaggac gacgtcgatc
     4141 ttctggccca ggttggggag gatcttatcc atcgcctcgg cctggggcat ggtccggggg
     4201 tagccgtcga gcatccagcc gcccttgacg tcgtctttgg ccagcctgtc cttgatgatg
     4261 tcgatgagga ggctgtcggg gacgagcttt cccgcctcca tgaacttctt cgcctccact
     4321 cccagagggg tgttgttcct cacgttctct ctgaggatgt ctccggtgga gatgtgaacc
     4381 acgccgtact tctctgcgat catcttggcc tgagtgccct ttccggatcc tggtggacct
     4441 agcagtacta tgttcataat atccctcctg tattccttcc gatgaacagc aactcttcga
     4501 gcccacgcct ccccgacgcc ctcgccctcc ggggcgttga tctcgcgtct ccatccaaag
     4561 ctttcgggaa cccgcccctg gggcggatgg catggccgct ggcagttgct ctcgtctccg
     4621 ttaaggagac tctcaacaac gttaaaaaac ctttctccgc cggattcaga ggagagctgc
     4681 cagggacatc gccgacccgg agaagagggg gatcgagagg ttgtcgtcga tgatcttacc
     4741 ccggaaggtg agggggatgg cgtcggcgaa ggtcgccgcc gcggcgccga cggtccggac
     4801 ggggagggtc agaacccccg ggccgacgaa gtagatcccc tcgagaaggc ttgcggcatt
     4861 ccccaccagg aggcagacgg cgaaggtccc cgccaccacc ggcatgggct tcgtcctccg
     4921 ccccccgccc tccatcatcc gggtcctcac cgccgacccc cggacgacgg atccgatgat
     4981 cccggaggcg gcgtccccca gggcgagcat cagcatcgcg gcgacggcga tgggggggga
     5041 gaatagggcg accgtcataa gggctcccaa gatgtagtag aagtagcccg cgaccctctc
     5101 cgcctcatgg tcccggaccg ctggaagccg gagcttcccg gagagcctcg cccgctccag
     5161 gagggccgcc accgccaggg ctgcggcgac gaaaccgatg gcttcagcct ttccgaagag
     5221 gaggagggcc gccgggaccg agagccccgt cagatggatc gccttcctcc tcgcctcagt
     5281 cgagagagcc gcccgccgct ctgacccctt ca
//
LOCUS       DASM01000024            4501 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_7380, whole genome shotgun sequence.
ACCESSION   DASM01000024 DASM01000000
VERSION     DASM01000024.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_7380"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          813 a         1413 c         1566 g          709 t
ORIGIN      
        1 atcttggcgc agaggatgca gggctggtgg gtgcagtaga gggtggcgcc gctggtgctg
       61 accccgtgga tggcggcctg gatgatggcg ttctgctcgg cgtggacggc gcggcagagc
      121 tcgtggcggt atccgctctc gaccttctcg cgggggcagc cgacctcgag gcagtggggt
      181 agccccatcg gggctccgtt gtacccggtg gagaggatcc tcttctccct caccaggacg
      241 gctccgactt ggtggcggag gcatgtagac cgggtcgcga cgacgctcgc gatctccatg
      301 aagtactcgt cgagggaggg gcgctccatg gccccggctt tgatccgatc cgatatctct
      361 ctttccgttc cagcctcaaa acttcttccg ccgagacaaa accggcccac ctccgcactg
      421 ccccccctcc gccatcctgg atcctcgccg tccccaccgc ggggcagcct ggcggggacg
      481 gccgagggcg gaaagttaat atcaccatct cgatcgatgg tgagcccatg acgattcact
      541 gggccgatgt ggcggccgat aagctcgccg accggggccc ccatacggtg gcgacgggga
      601 tcaccccctc aggccagatc cacctcggca atatgcggga ggtcatgacc gccgacgccg
      661 tctttcgggc gatcctcagc cggggcggcg aggcccggct gatatacata gccgacacct
      721 acgaccccct ccggaggctc tatcccttcc tgccgaagag cttcgaggag cacgtgggta
      781 agcccctctc cgagatcccc tgccccgagg ggtgctgtga gagctacgcc gatcacttcc
      841 tcctcccctt cttcgactcc ctggagaggc tagggatcag gcccgaggtc taccgggcag
      901 accggatgta caaggacggc ctctacgtcg acgccatcaa gaccgccctc ctgaagaggg
      961 acgatatagc gaggatcctg aaggaggtct cggggaggga cctccccgcc gactggtccc
     1021 ccctcgacgc catctgcgag agctgcggcc ggctgaacag caccaaggtg acgggattcg
     1081 acctcgacgg cgagacggcc gagtacgtat gccggtgcgg ccaatcgggg acggcgagct
     1141 tccggggcgg gggtaagctc gcctggaggg tcgactggcc cgcccggtgg tcgatcctcg
     1201 aggtcaccgt cgagcccttc ggaaaagacc acgccaccgc cgggggatct tacgacaccg
     1261 ggaagaggat ctccgaggag gtctatgggt accccgcccc ctaccccgtc gtctacgagt
     1321 ggatccacct gaaggggaag ggggcgatgc actcctccac cgggatcgtg gtgacgatcc
     1381 gggatatgct ggacgtcgtc ccgccggagg tcctccgcta cctgatcatc aggacgaagc
     1441 ccgagaagca catcgagttc gaccccggcc tccccctcct caccctcatc gaggagtacg
     1501 accgcagggc cggtgaggag agggccatcg agctatcgaa ggtcggggcg gagcaggcta
     1561 ccgccatacc ctaccggcac atggtgacgg cggtccagat cgccggagac gatcccgaga
     1621 agctgatggt ggtcctggag aggagcggct acgacacctc ggagggggac cggatcctct
     1681 cccgggccgc caacgtcagg gcctggctcg acaggtacgc cccccccttc gtcaagttcc
     1741 acctccagga ggccctgccg acgaagatca agaacgtcga acccgagctc cgggaggccc
     1801 tcggcgccct ggcggggagg atcgagggga agggcgccga ggagatccac aacgaggtct
     1861 acgccgtcgc cgaggagagg gggatcgagg caaaggagct cttcagcgcg atatacgtcg
     1921 ccttcctggg gcagaagcag gggccgaagg ccgggtggtt tttggcgagc ctcgacggcg
     1981 agttcgtgcg ggagaggctc ctcgcagccg ccgccgatta ggtcaggcgg gatggaggag
     2041 gtcgaagaat tgacggcggg tccagagggg accgaggggc aaaggccatg ccccagggtc
     2101 ggcagagaag gaggaggcat cgtgagggag aaaacggagg ataaggtcgc cgtctacctg
     2161 gccggccccc tcttcagccg cgccgaggtg gagtgggcag gatctctcaa gagggagatg
     2221 gaggcggccc tgggggagag gattgaggtg atctggccct tcgaggtcgc ctccggatcg
     2281 aagggggaga tcttccgggc gaacctggcg gccctgtccc ggtctcccct gatggtggcg
     2341 gtcctggacg gccctgccgt cgacgacggg accgcctggg aggtgggctg ccacttcgcc
     2401 ctcttcggcc ggcgggcgat cgggatcagg accgacgtca ggaaggctgg cgaggccccc
     2461 gactccaggg tgaacctgat gatcgaggag gcgtgcaggg cggtggtggg ggaggtcggg
     2521 gtcctcctcc gggagcttgc ggccgccctg gaggaggtct ccccgccaga gttgaacgcc
     2581 atcggtccga aggccccaga gcctgcagaa tgacgacggc aaggccgatc ctcaacgagc
     2641 ccctcctcct ggtggagggg aggagaaagg tgctggtggc cgcagacctc cacataggga
     2701 tcgagcacca gctctggctc ggcggcgcca gcgtccccag ccagaccgga aagatgctgg
     2761 gggggctcaa gctccgggtc ggcgaggtgg agcccgatcg ggtgatcctc ctcggggacg
     2821 tcaagcacaa cctcccctgg acctcctttc aggagaggag cgaggtcccc tccttcctcc
     2881 aggcgctatc cgatatggtc gaggtggaga tcgtcgccgg aaaccatgac ttggggatag
     2941 ctgagctcgc cccgccggag gtggccgtcc atccccccgg gggggtggtc atcgactccg
     3001 tcggctactt ccacggccac acctggcccg acccggaggt cttcagggcg gagatcctgg
     3061 tggcctccca cctccatccg gcggtgaggc ttgccgaccg gatcggctcc ggcccccccg
     3121 agagggcttg ggtgaggacg cccctctccc gggaggggct gagggaggag tacggcaggg
     3181 atctcgaggg acccgagatg acgatcgtcc ccgcctataa ccccctctgc gggggctacc
     3241 ccctcgacgc cgccacccgg gaggaccggg ggccgattcc taggatggcc cgcctcgacc
     3301 tctccagggt ctacctcctg gacgggacgg acctggggag gctcgacagg atacggctcg
     3361 tccagacgaa gaggaccggg aagctccgct gaagttatat ctccgggatg cttcaaccaa
     3421 ggcatggaag agagagagag gcttctggag cttttgagga cgagggcgct ccgcagaggc
     3481 aggatcaccc tctcctcggg gagggtgagc gactactacg tcgacgcccg ggaggtagtc
     3541 ctccaccccg agggggcgta cctgacgggg aggctcctcc tggagaggat cgaccccgag
     3601 gcggtcgccg tcgccgggat gaccctgggg gcggacccga tagtagcggc gatagccgtc
     3661 gtcggccacc tccaggggcg agacctctcg gccctgatca taagaaagga gccgaagggc
     3721 cacgggacgg ggaagtatgt cgaaggtccg accctccccg aggggtcgaa ggtggcggtc
     3781 gtcgacgacg tcgtcaccac ggggtcgtct ttgataaagt cgattgagcg gctgaggggc
     3841 gagggatacc ggccggtcca ggtcctcgcc atcctcgacc gggaggaggg cggaagggag
     3901 aggctggagg cctccggcta cagcctgaac tgccttttca ccaaaggtga cctcctcgcc
     3961 agataggggc gttagctgac gttcagctgc ctattgctcc gatcgccgca gacgatgtgg
     4021 cgagacctcc cctcgggatc ggtgtagatc acctgggatc tgacgtggta gaggcccgga
     4081 gcctcccccc tcaccagata tgcgacctgc tccgagcctc cgggcgagag ggtcaccgac
     4141 gagggcctcg cactctgggc cgagaggccg gaggggaccg ccgggacgac ggagacgttc
     4201 agagcggact cgccggtgtt gccgaggcgg agcatcacag agacttcctc ccctaaacca
     4261 gcatggtagc ggtccaattg ctgctctacg tggatcccca ccaccatgag ggacagcagg
     4321 accggaacca tgagagccgt cttcattgaa ccccgatttt gtgacggcga gatttaaagg
     4381 tagctttcat ataagagcta cgtgagacct ccgcgtcacc tttccgtccc ggaagggaca
     4441 caaaaatact tatcgtggcg gagctaggga taagatcgat ggacgtttcg tactcggtct
     4501 g
//
LOCUS       DASM01000025           11760 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_1916, whole genome shotgun sequence.
ACCESSION   DASM01000025 DASM01000000
VERSION     DASM01000025.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11760)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_1916"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2053 a         3830 c         3629 g         2248 t
ORIGIN      
        1 tgcagcagca tgatcatcgg cgaagacctt caccaggccg tcgtcgacgc cgatgtggac
       61 gtccccgtcc tcgaggtgga ggtccacgcc ggctaccggg acaataccaa aggggtgatc
      121 ataaccctgg aggcggccct ggagaagggg atcatagacc gggtggagtt cgagcggcag
      181 aggttcctcc tggagaaggc gacggaggtc gaacggctcc acggggcggc cagcagcgaa
      241 tacctcgccc ccgagagggg cgacctgaag tacaaggccg cggagaggct tttagatctg
      301 ataaaagagg ggaagagggg gatcaacgtc ctcaacgcca agaaggagac cgcctacatg
      361 ttcgccgaca tcaacgccgc cgtggctgag gtggccaagg cccgcggcgc cgaggtcgca
      421 accctcgcaa acctcgacga ggagaagggg ctggccaagg tgagaaagga cgcaaagaac
      481 atccttcggg ggcttgagga gcgaggcgtc gaggtcgaca ggataatcgg gggcctcgac
      541 gagtacccca tcgccggaga tgaggtggcg aggtggcttg cgggtcaggg gagctacgac
      601 ttcgccgtca tcaccggcgt cccccatgct gtacccatcg acgcgctcaa gggcatggag
      661 atcttctcca tcaccaacgg cccccggcag gtccggcccc tccgggagct gggccaccag
      721 cacgtaatgg tggagatcga cctccatccc aagacgatgg gggtatccac gatcgtagaa
      781 tcggagtttg gagccaccct ccggggcctc aacgaggtgg gatgagggga gaaagggtcg
      841 agggatgggg ctggatgccc cgcctctctt ctcctcagaa cgcctttgga agctccgtca
      901 ggaagacgta gatgatgaag ccgagcatcc cgatgagggc gatccccagg atcgatatct
      961 tggagatcat gaggaactcc tcccgggtgg gcttcctcgc cagcttcagc actctgatgt
     1021 aatccttgta gtcgaacttc ggggcggcga tcttcacgtt catatcgccc agcctctcgg
     1081 ccagctcccc cttgtggccg ttgactttgt tcttgtggtt cttagctttc tcttcggaca
     1141 ttcagcgggc gaagggggct tcctggtgaa aaagatttcg atcaaggcgg cgatgccgcc
     1201 cgccccgagg gccgatccga agccggaaag gtttttaacc gaccgatccc tggttgagag
     1261 gggttgaggg atctatcatg gactggagcg tccttttggt catcgtcccc gtcgtcgtcc
     1321 tcttcctcta cggcatcgag aggttctccg aggaggtccg ccacgctgcc ggtgagcggt
     1381 tctggaccat cctccagaat gctacccgta cacccctccg gggggcggtg gcgggggctg
     1441 gcgtctcggc gatcacccag tccagcaccg ccaccaccgt catcgccgta gggctcgtca
     1501 acgccggggc gatctccttc ctggggtcga taggcctcgt catcggagct aacatcggaa
     1561 cgacgatcac ggcccagctc gtagccttca agctcaccgc cttcgccccc ctcttcgtcg
     1621 tcggcggctt cctcctcagc ctcatccggg ggcgctaccg ggtcttcgga aagccgatct
     1681 tctacttcgg cctcgtcttc ttctgcctga acatgatctc ggcggagatc gcccccctcc
     1741 agggtgaccc ggagatcatc gccctcctct ccatggccga gaacgtcctc atcgggatcg
     1801 cgatcggctt cgtcatcacc aacatattcc agtcgagctc agtgaccgcg ggcctcgtcg
     1861 tcgtcatggc ccagagcggc ctattgaccc cggcggaggc gatccccatc atcctggggg
     1921 cgaacatcgg gacgacgacg acggggctcg tcgtagccat caggatgaac gccgccgcca
     1981 agaggacggc ggcagcccag ttcctcttca acctcctggg ggtcctcctg gtcctgccgc
     2041 tcctggggcc cttctcagcc ctcatcgaag ccctgggcgg cacctccgcc cagcaggtgg
     2101 ccaacgccca tctgatcttc aacctctcgg cagccctggt cttcctcgcc gccatcaggc
     2161 ccttcgggag gatcgtgatg aagatagtcc cagagaggga cccggaggtc gtcttcgtca
     2221 cccgccacct gaccgttcct cttcccgagg acgccaccgc cgccaccgcc cagatggagg
     2281 cggaggtcgt tcacatgctg gaggtctccg aggagatcct ggagaagagc ctcgaggtgg
     2341 gggccgggag gacggaggcc tgcggcagga tcaagcacct ccgggattac gtggagtacc
     2401 tcaaccacca gatcatcgac gccgtcgtct ccatggaaga tagggggctc tccgtcgagg
     2461 acgccgcccg gatctcgatc ctcgcccgga ccgccgacct cgtcgggtct atggcagagc
     2521 agaccgtcgg gatggcgagg aggtttgagc agatgaaaga gaagaatatg cccctatcct
     2581 ccgaggccct cggagacctc caggagatgg ccaccccctg ccgggagaac ctccagcatc
     2641 tgatggcgac cttccccgat atgccggcgg aggtggatga gaagatgcgc tacaacgacg
     2701 agaagctccg ccagctcctc aactggtact ataagattca catccggaag gtggccggaa
     2761 gggggagcag cgccgatggg atcttttcag aggtcctctt ctccctggag aggatcgggg
     2821 cgatgatcag ggagctgcgg aagaccgccg tatccatgag gacgccggcc acccccttga
     2881 cgatccccga catcatcacc ccgcccggga acggggcggc ggaggatccc ctctaaactg
     2941 agatcctctt cgccaccgcc tcggtgacct cctccgtcga ggagcagccc cccaggtcgg
     3001 gggtggcgat gccgctggca acggcccacc ccaccgcctc ctcgatcatc cgcgccttct
     3061 cggcctcccc gaaccactcc agcatcatgg cggcagagcg gatggctccg atgggatttg
     3121 cgatcccccg gccggcgatg tcaggggcgg agccgtggat cggctcgaag agggcgtacc
     3181 tatcgccgag gttcgcgctg gggcagaggc ccaggccccc gacgaccccg gcggcgacgt
     3241 cgctgaggat gtccccgaag aggttcgtcg tgactatgac gtcaaacctc ttggggttca
     3301 tgaccagggt gtagccggcg gtgtcgacga gcatatcgtc gaaggggagg tcccaccgcc
     3361 gggccgtctc ccggcatatc ctcaagaaga gggtgtcgct cttgaggaca ttggacttgt
     3421 ggacgatggt gagcctctcc ctcttcgaag cgatggcgca ggccctctcg gcgatccgga
     3481 gagaaccccg ggaggtgatc accctcaggg tcgtcgcctc ctcctcgtcc agcttctcga
     3541 cccccgagta catcccctcg gtgttctccc ggacgatgac gaagtccagg cccggaaccg
     3601 agaagggcct gatgttggcg tagaggtcga ggcccttcct aagggccagg aggacgctcc
     3661 tgtactcggg gtccggaggg gtcgtgatgg cgccgaataa gacgcagtgg cacgtcttga
     3721 tcaattccag atcttcatcg gccatcgccg cccccgtcct cttccaccgg ctgtacccga
     3781 gctccacggg gacgagttcc agatccgccc ccgccgccgc gaggacggcc aaggcgccgt
     3841 cgatcacctc cggcccgacc ccgtcccctt tgaccaaagc taccctcttt tcagctcctc
     3901 tgctagacat cttatcgcct ccgcgatctt cggggccccg tccccccagt ggacgacgac
     3961 ccccaattgg cctctagacc aactgatccc gctccgctcc gacttgcagc accgggtgat
     4021 gaagggcccc ttcggagcca gctcgtcgtt ggagaggggg caggtgttca gaaacctgaa
     4081 gttcttaccc gggtacatat cccggaacac ctccctggag acctcgcagc cgacgatcag
     4141 ggggtcgtcc acctcgactc tgtctctatc cagggacctg cccagccccg aggcggtgca
     4201 ggggaagtag acgtcatcgc cttcgaaccg gcggaggtcc aggaccctct ccctgaactt
     4261 aagggtcaga tcgcccagga caccggcctc caccagcctt ccgactacgc ttaccagcca
     4321 cgacggccgg ggtgggacga cgtccaggat ctcgatctcg gtgacggcgg cggggtcggg
     4381 gtcgtggacg aaggtgatgt gctcgtcggg gccccgaaag atcaccgtat tcaccctccc
     4441 cttgcagagg gagctggcga gctcgatgag catcgcccgg ttcctggtgt ccaccgtctc
     4501 agggtagacc aggatcgtgt ccccgctggc gatcggctcc atcgacctca ccggccgcat
     4561 aaatcccacg ccagcccgag cctccacccg atatattgtg atctcgcccc caaagtggat
     4621 cagatacctg gtggagaagt agatggggtc gccgggcctc gcctcgctcg ccgcacccac
     4681 gtacttgtaa tcctcaggga atatcaaagc gctccttcct tctcctttct ccggtgggcc
     4741 acaagacccc cgtcctggag gatttcgagg agaaagtcgg ggatcttcgt tcccagaaac
     4801 gtcctccccc cgacggtcac cttccccgcg gcgaggtcca cctccaccac ctctccctgc
     4861 cgggcaacga cggccgcctc caccagcggc aggcccacgt ttatggcgtt ccgatagaat
     4921 atccgggcga aggacttggc caccacgcaa gagacccccg cctcccggag ggccagaggc
     4981 gcctgctccc gggaggagcc gcagccgaag ttcttcccgg cgacgatgac gtcccccggc
     5041 cgcacctgct tcgcaaactc cgggtccagc ccctccatgg cgtggcaggc cagctcccgg
     5101 acgtccctgg tcctgaggta agggccgggg attattacgt cggtgtcgac gtcgtcccca
     5161 tagacccacg cctttcccct gatcatgggc gagagttaga cctgcaacgg tatatggctt
     5221 tcctctgaag aggtgagcat cgccggcccc gcccacggtg ctgaattgtc cctagacctg
     5281 tagcgcatca aaaaagctgg aaggtcagta gacctcttcc aggtcctcga tgtaggcgaa
     5341 ggcgtcgtcg agctgctttt tggccctggt cttccggccc ttctcgtcct ggatcgcctg
     5401 ggcggcctgg gcgtagagca tctccaggtc ggggacctgg ccgagggtga agacggagag
     5461 gaccttcggc cgccctttct ttatgacgat acccttgaag acctggccga tcttcgtcga
     5521 gagcttctcc acggacttgg tgatctcgtc gacgtcgagg gagtccctcg acccctggat
     5581 gatgatcatc gccctcgcgg cgtcctcgta gacgtcggcg ggatagagga gccccgacgg
     5641 cttgagggag ctctcgatcg ccgaggtgag ggaggcggtc ttcttgtccg actcgtagaa
     5701 gccgagggtc cccatcccca ggcctccctt catcaccgtc ttgaagtccc cgaggtcggt
     5761 gaccgagagc atctcgctat ctaaggactc aatgaggaag aggagcctct ccgccaccat
     5821 ctcgttgatc ttgtcgtagg cggtctttat gtcgccgctc agccgcttca ggtactggtt
     5881 gtccactagg atgatcccgt cggactccac ctccatgatg tccctcatgc tgaaggcggc
     5941 gttctggagg tatatcgacc cctcctcccg gaaggggagg accatcaccg ggatgacggt
     6001 gatcttcttg taacgctccc tcagctcgtg gacgacgagg ggggtgaagg aggagccggt
     6061 ccccccggag gcggaggtca tcacgaaggc gatgtcgaag tcccccctcc gctcgatctc
     6121 ctccatgatc atctcgcggt tctcccagaa gccctgcttt ccgatccccc ggttggctcc
     6181 gactccgtgg aggttcggga cgtggagccg gtctttggcg gcggtgaacc ggagctcctt
     6241 gaggtcgttt acggcggtgt tgatggcgag ggtctcaacc cggctgggaa acttctgccg
     6301 cgaatagtac ttggatagcc tggaggagcc tccgaaggcc tcccggttga cggcatccag
     6361 gatcctgttg ccacattggc cgatccccag catcagtacg ttcaacatat atctcagtcc
     6421 ggaattggtg gatggagagg cgccataccg gcgccccgcg gcgtttttta aggtcacgcc
     6481 ctccgcgccc ctccccgggg tcccgagggc tcatcggatg tatcaactga ctccgactta
     6541 atatttatat tctttgtacc gtcggaagag ccaagccgcc aggatgacgg cggggacgat
     6601 gggaaggagg tagaggaacg ggagctccct ctccagcacc gtcacatcga aggaggtccc
     6661 tcgctgcatc atctcccctt cggcctggaa gctggcaacg aggtcgatgg tgtagtttcc
     6721 gctccgggac ccgcggaggg tcgcagagta gctctcagat cctcccgccg ggatctcgaa
     6781 gaagctctga tcgagggccc cctccaccac ctccaggtcg gatccgacgg acttgaggtt
     6841 cgacctcacc tccaccctgg aagcgctggc atccccctgg tttcttatgg tgacggtgaa
     6901 cgtcccctcc ccgccatagc tgataggacc tgcggaggcg acggatagga gcagatccgg
     6961 cttcctcttg ggcttgatgg ttacgctggt ggcctttgag ctggcggagg agaccgcgga
     7021 cttcgagtcg gcgtacctga ccaccgtcgc cgggatcttc gcctccccct ccgtcgccgc
     7081 caccattgag tagacggcga ggtcggactc gccgacctgg aggctgttct tgatcttggg
     7141 gggatatcca gcgacgtacc tgaacgccgg cagcggcggc gagtcctgga ccgccacatc
     7201 ccgggcggta tcgttcccga cgttcgtcac ggtgatcgtc accttcacct cgtctccgac
     7261 gctgaggtcc gttctgtcca gggaggtcga gacctccagc ttcgggaagg tcctgggggc
     7321 gagccccctc tcggtgacgt acttctgggg gtatccgtag ctcgaccggt acttcgccct
     7381 catcctgacg gtatcgtcgt cgaagatgtc gatgaccgtc agggtgaggc cgccccggtc
     7441 ggccccctca tcgaagttgg cgtccctgtg atcctccatc accctccagt cctcaaaggt
     7501 gccgaccctc ttccagacct tcacctcgat caggccgtct accatcgata cgacgtttat
     7561 cagctccact cgatagtctc ctatcctcac cccctccccc accctgaggg agaaatcccg
     7621 ggatgtggta tcctccacct ggtcgccccc atcgtcggag gcggcttgtg ccgccggaat
     7681 ggcggccaga aggaagagag ccatcgccac gagatagatc ggtcgcattc caccccaccg
     7741 aatttggacc tcgttaatag gacggtcgat ataacgttta ccatctatcc ggccgggatc
     7801 agacctcgat ctccaggctg aagtcgatcc atggggcgga cctggtgagg gctcccaggg
     7861 agatgacgtc cactcccgtg gcggcgtatt cggggaccgt ctcgggggtt atcccccccg
     7921 acgcctccag gatcacccga tctcggagcc cctcccgggc gagaagctcg acccccttcc
     7981 ggatctcatc cggcctcatg ttgtcgaaca taattatgtc cgcccccagc cgagccgcct
     8041 tcaccgcgtc ggtgaccccc tccacctcca cctcgatctt ctgggtgaag ctggccatcc
     8101 gcctggcggc ggccagggcc tcttcgaggc tcatcagctt gaggtgattg tccttgatca
     8161 tgacggagct ggagaggtcg aaccggtggg ggtcgccgcc ccccaccgcc accgccctct
     8221 tctcgaagga ccgaaaaccc ggcgtcgtct tccgggtgga ggcgacccgc accctcccag
     8281 ccagggccgc gcagtcccgg gtgagggtgg agatcccgct catcctcccc atgaagttca
     8341 ggaccagcct ttcggccctg aggatcgccg ccgctggacc ccggatctcc atcaccctcc
     8401 tccctgcggc cacctcctcc ccctccggga cgagccccga cccttcgagg ccgaagtaat
     8461 tgaggatggc gaaggcctcc gcgaccccag aaacgacgca cctctctttc gccacgatcg
     8521 cagccttcgc atcaacttcg gggactgggg ggatgtcatc ctccatcgcc cccagatctt
     8581 ccctgacgaa ccgctccagc tcagatggaa gcataccgca aaagttttgt tgtcccattg
     8641 atttataggc tccgatggtt ctgaagatag ggctggtggg atgcggcgcg atcggtctgg
     8701 agatcgcaag agcgatagac agaggcgagg tcgaggcgga actggtggcg gtcttcgatc
     8761 ggaacggaga tagggcgcgg gagctgttga aggggctgag gggaaagccg aggctcctgg
     8821 agctggagga actggtggag ggctccgata tcgtggtgga ggctgcctct cagagggcgg
     8881 tccccgaggt ggccagggca gccctcggga gggggcggga cctgatgatc atgtccgtag
     8941 gggcccttct ggaccccggc ctccgccgga cggtggagga gctcgcgagg gataggggct
     9001 gccgggttta cctcccctcg ggggcgatat cagggcttga cggcctcaag tccgcggggg
     9061 ccggaaggat agacatcgtc accctcacca ccaccaagaa ccccaggggg tttgcggggg
     9121 cgccctacat cgaggagagg gggatcgacc tctcggcgat ctccaagccg atggtgatct
     9181 ttgaggggcc cgccgaggag gcggtatcgg cctttcccgc caacgtcaac gtcgcagcta
     9241 ccttaagcct caccgcccgg ggggcgaggg tgagggtgag gatagtggca gacccagata
     9301 tcgaggtgaa catccacgag atatgcgcgg agggggactt cggtcggatc accacccggg
     9361 tcgagaacgt cccctcgccg aggaacccca agaccagccg cctcgccgcc ctatcggcga
     9421 tcgccaccct ccggtcgatc gtcgagccgg tgaagatcgg aacctaagcc caccacttgg
     9481 ccggctccgt cttccccggc tccttcttcc ggatcactat ggtatcgttc tccatcctgt
     9541 aggggaggtc gtcttcgggg ttgtaggtgg cgtatcgggc caggtcgagg cgggcctcct
     9601 cgatatcgac gatgccatcg gcgggggtca tcagggggcg ccagtcgacg acgcagctgc
     9661 tccccttggg tatccagtag ggatcgtccc ctaccccgtc gccgtcccgg tcccggcccc
     9721 ggtagtcgtc gtagtagttt ccgacggagc cgttccagaa gttgtggctc gcgttgtcgt
     9781 agccggtgat gaggttgcgc ctgaagttgt ttttgtatac gaggttgttc tcgttcttgt
     9841 ccagccggat gccgttgctg ttctcgtaga tctcgtttga gaagatcagg ttgaacttgc
     9901 agcccctggt caggtcgagg ccgttgaggt tctcggagag gacgttgccg gccaccaggt
     9961 tgtaggtgct gttctcctgg atgctgatgc cgatcccctg gaagccgtac cctcctccgc
    10021 cggtcttcct cgcctcatta ttcaggatcg agttgttcga tcccgaggcg accaccatcc
    10081 cgtagtatac gttccggtcg gcctcgttct ccatcaggtc gcagaatgag gagttccaga
    10141 gaaagagccc ctgccagttc tcgataaaag agttcccccg gagggtgcat ccggtggagt
    10201 ctttgagcat aatcccggcg ccggggtttg ctatgacctg gttcctctcc agggagaagc
    10261 cgcttgaggc ttctatgctc gccccgtact tcttgcaatc ctgaaaagtg gatcccgcca
    10321 cccgtccccc gctcccctct tggatcaata ggccgatatc acatctctgg atggacgaat
    10381 ttaggatggt gaagtttctg gcatatttga ccaggatccc cgcctcagat ccgttcacga
    10441 tcatatcttc gaggaggacg tcgtttccat ccaccaggat ccccgagttg ggctggttca
    10501 gggagtactg gggcttgcgg ctcgagaggt ccatgtaaaa tctgaacttg tcctcctggt
    10561 tctttgggac gccgtcgacc tggaagccca gaatctgcac gccgtggccc ctgatggtga
    10621 tcgccggatt ctgggtcttg gcccggaccg acggacgccc ctcccccagg atggtgaggg
    10681 gcctgtccac ggtgaagggc tggtagaccc cggaggggac gatcagagtg tctccggggc
    10741 tcgcccccgc gatcgccgcc tggaggtccc cggcgacgag gatctcagat gccccagccg
    10801 tacccgcgag ggcgaatagg agaacccata tcgatatgaa ggtcgatgcc atagccgggg
    10861 ctggggccga tgggggatgg ctcccccatg cgacggggcc gggccccgga tcaatgtctg
    10921 aagtggcgca tgccggtgaa gaccatcgcc atgccgtgtt cgtttgcagc ttctatcacc
    10981 tcagcgtccc tgatggagcc tccgggatgg acgacggcgg tggcccccgc ctcgaaggtc
    11041 cgatcgaggc cgtccctgaa ggggaagaag gagtcggtgg ccgccaccgt gcccctggta
    11101 tccaggccgt gctcttctgc cttctcggcc ccgaaccggg ccgagtcgac cctggagacc
    11161 tgccccgagc cgatccctat cgtctggttc gcggtggtgt agacgagggc gttggacttg
    11221 acgaacttgg tcaccttcca ggcgaaccgg agggccaggg tctcggcagg ggtcggcccc
    11281 cgctccgtca ccaccttcca atcctttatg tccccggtgt cgtagtcctg gacgagaagg
    11341 ccgccgaaga cgctcctcga ctgcctcccc gcgtagagcc ggctccggcc ggataggagc
    11401 tcggatatct ccaggatcct ccggttgggc ttctcccgga ggatctcgag ggcatcgggg
    11461 tcgtagccgg gggccagcag gacctcgata aagtggtcgc gcatctcctc ggcgaggtcc
    11521 gcatccaccg gctcggagaa gcctatgacc cctccgaagg cgctcttggg gtcggtggcg
    11581 agggccctcc gataggcctc gatctcgttt gagccgtagg cgacgccgca ggggttcgca
    11641 tgcttgacga tcaccgccag agcccgctct tccctcaccc cgtcgtactc caggaggtcc
    11701 atcagcatct ccagggtcgt ctcggcgtcg acgacgttgt tgtaggacat ctccttcccg
//
LOCUS       DASM01000026            6171 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_85968, whole genome shotgun sequence.
ACCESSION   DASM01000026 DASM01000000
VERSION     DASM01000026.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_85968"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1291 a         1759 c         1983 g         1136 t
ORIGIN      
        1 ggtggtgggg gcggagaagg tccccgccga gatctacgag atggctgact ggaacgtggg
       61 ggtcggaaac cagccccact cggaggtggc cgccctcgcg gtcttcatgg accggctctg
      121 ggagggggag gagctggcgc gggagttctc ggggaaaata gaggtcgtcc ccagcccccg
      181 ttacaagacc gtactcgagc ggcccgaggg tgaggggggg tgaggggaga tcggctccgg
      241 cgggtcctcg tcgtcggcca gtccgtccgg ccgatcgccc tctcggcggt ccgggctggc
      301 catgccgtct tcgccgcgga ctgctactcc gacctggacc tggtggagtc cgtatcgagg
      361 tccgtccacc tcgactggga tccggagagg ccggaggccc taccccggaa ggcgaggccc
      421 gtcatagagg cggcgatcga tgccggctcc atcgactgcc tcgtcctcgg ccccggggcc
      481 gaggggatga aagtcgatgg ggtcagagta ctgaacaacc cgccggagaa gttcgcagag
      541 gtctccgaca agctccgcct cgcccgctgg ctagaagagg aggggtttgc ggcggttccg
      601 acctggcggg tcggcgacgc cccgacggcc gacctccccc tcatggtcaa gccccggcgg
      661 ggggcgggcg ggggcgagaa ccggctcgtc tacaccgagg aggagctcgc cctcctcgaa
      721 ggggatctga tagtccagga gttcgtccga gggacccccg ccagcgtatc ggtgatcgcc
      781 gacggggaga ggtcggtggc gatctccgca aacgagcagc tgatcgggac caggtggctc
      841 ggcggcggag gcttcaggta ctgcggcaac atatcgcctc tagaggctcc cggggccggc
      901 tccgcttcgg cggcgatgat gaggaccgcc gaggagatcg cctcccgcct ccagctcgtc
      961 ggatccaacg gcgttgactt catcctctcg gcctctggac ccctggtcct ggaggtgaac
     1021 ccccggttcc aggggagcct cgacgcggtg gagatatcga cgggcctaaa cctcttccag
     1081 gcccacttcc tctccttcga cggcgtcctt cccaaaaggc cccagccccg gaggtctgga
     1141 gggagggggg tcttatatgc tgaagatggg ctgaaaatag atggggacct ccgggccgtc
     1201 aaccgacgga tcgccgacgt cccccgcccc ggatcgtccc ttaagcgggg cgaccccgtc
     1261 acgtcgatcc tctcctcggc ggcggaccgg gatggagccc tggcccttct ccggaggagg
     1321 agctcatcga tccgcagggc cctgagaggc ctctgaggct caccaggaca tgacccggta
     1381 gaaccagtgg gagattatgg tgatgaactt ttcaaagccg cttcctttct ccagcttctt
     1441 ccgatcctca tcccggtact tcttatcctt catccaagac atgatttttg ggttgtcgat
     1501 ctggagatat aacgatatcg gtctttggcc caaaccggca ggcagccagg aggcggcctg
     1561 gccatccatc tggagcttcg gcggaccggg acggcggctt tgggggagga tatcggagtt
     1621 tttggccggc ccctgaagaa gacgtcggcg cgaaaccaaa atctttatga tacataagag
     1681 agggtctcat ccccagtgat agatatggat atagtaacca ccatccaata ccttcaggct
     1741 ctgatcgtgc tggtgctctt ctttgggatt ctggccgtcg ggttcaaggt ctggcgctgg
     1801 aactacgcct gaccccgccc cgaggatctc tttttttcgt tccgccgccc accctcagcc
     1861 gtagcttagc cggccctcgg ctagtagctt cccaggacga ccaccttcgt cccctggagg
     1921 tcgagcctgg agccctggcc ggggcgtatc tggaccgatg cggagctgaa cttgtacccg
     1981 gcggcgtaga ctgagatcga atgccatccg ggggcgaggg atgcgccgta gcctccgtcc
     2041 cgatcggtgg tgacggtgaa gatcccgtcg atccggaccg tcgccccggg gacggcggct
     2101 ccgctcctgt cggtgacgac cccctccacc cagcctgctt catacgctcc gggttcggcc
     2161 tcgggggacc agcctgtctg gtaccccacc accgtccccg ccgctgagac cgccccggtc
     2221 cagacccggg ccgtcgaggg gctgaaggtg aacccctcct tctccgccgt cacggtgtag
     2281 ctccccgagg ggacgtccat accgtagtag ccctgggggt tagtagcggt ggtaaagatc
     2341 ccgcccgccg atcctgtcat cctgacgagc gcccccggga tcccgttccc gcgggtgtcc
     2401 acgacccttc ccaccaggcc gtaaccggtg gggtagccgg cggagctgac ggctaggggg
     2461 acggggttgc tccacctgct ggtgttgaac tgatagcggt gttcctccag ggtctcagca
     2521 tattggtcga tccggtacca gccccgggtc accagcccct ggtacaggat cctcctcccg
     2581 ccgtcgtaga cggagagcgg cccccactcg ccgacgtaga gccagagggt gtagggggag
     2641 ccgaggccga cgaccccctc cgtcaccagg aggcgaccgg actggaggta gagctggttt
     2701 ctgctcgaga catcggcgcc gatggagagg ttgtattccg gggcgaaggg atcggcggag
     2761 acgatctgag gaaggtctcg gctctcgggg gagaaggggt cccagggggg cctcccaggg
     2821 taataggtgg attcgtaacc tgggccgaag gggtcccatt cgggcctatc ggcggcgaag
     2881 gggtttggaa gaaaatccag catcgtcatc tcgtcggcgg cggtgccgcc ggcgataaag
     2941 gctgcagcat agaccagcac caacatccaa aacgcagatc ctacgaatga tctcaaggct
     3001 tgaacctccc caaaataaac gactaccgcc atcgacttaa agattgcggg tttggagagc
     3061 cgaccgtatc cggcgaaccc tcaccgccgg ggacggagga tctttaggat ccgtccccca
     3121 gggctcgaag tcctctgaat aatcttcctt ttacctcgcg ggattgcgaa gggcagagcg
     3181 agaaggcccc acccttcagg gtggggatga aagcggagcc ctttgccctt ggatccatct
     3241 aaagattact atttaagaaa taaagacaat ctacatttcg atgcttaagg cttacaagtt
     3301 caggctctat ccaacaaaaa cacagataag caagatggag agaacgctgg acttgtgcag
     3361 atgggtctac aatcaaaccc tagcataccg gaaagatgcg tgggagaagg aaggcagatc
     3421 accctcaaaa tatgaaacca acaaccttct acccaattgg aaggtcgaga agccagatct
     3481 taatgaggtg ttctcccagg ttcttcagaa cactcaggag agagtagatc ttgccctgca
     3541 agccttcttc cgaagagtta aggccggaga gaaacccggc tatccaaggt ttcgaggaaa
     3601 tgggtggtat gattcattca cctatcctca atttggtttc aagctcctat ccgggaagct
     3661 tcgactttct aagatcggag atatcaatat caagctccac agacccatcg aaggcaagat
     3721 caagaggtta accgttcgca gatcttcgac cggaaaatgg ttgtcctgct tctccgtgga
     3781 gatcgacgat ccgcttaagc caccgtggaa agacggatta tcggtaggca ttgacttagg
     3841 gctggaaagt ttcgccacga tgtccaacgg agagaagatc gacaaccccc gtttctttcg
     3901 atctgaaaag aaggcgttag ccaaagctca gagacggcta tccaaatgcg agaagagaac
     3961 tcctgagagg agtaaagccc tcaaagtcgt ccaacggatt catgagcgga tcgccaatcg
     4021 aagatacgat tttgctcatc agataagcaa tcagttagtc tcgaaatatg gtctgattgc
     4081 tttcgaagat ctaaatatca ctaagatgct caaaaaccac aacctggcta aaagcatatc
     4141 tgacgtagct tggaggatgc tagtaacaac cacatcatac aaggctgaga gcgccggttc
     4201 gaaggtggtt ctagtcgatc ccaggaatac gagtcagcta tgctctaggt gtggggtaaa
     4261 ggttcccaag tcactgtctg atcgtgtcca tgaatgtcct cagtgtgggc tggtcatgga
     4321 ccgtgacgnn atgcaatctc ttcgcaaacg cgatagaagc ccctcccttt agggagggga
     4381 gcattcacga aaacgcttaa ctcagaaaag gtcgatttca gggtgccctg gattggatcc
     4441 gtcaactaaa ggcggacctg tatctccctc cccggcgaac gaggcggtgg aggcggcacc
     4501 agggaacaca tatataagcc cgaaagaagg ccatcatata aagtctatat tgtttggagg
     4561 agagtcatga accaccgtag ggctggagga ctgctttgcc tgctcctggt caccgtggcg
     4621 gcagttctgc caatgggagc gttggcgcaa gtaggggatg ctccagatga atcggtcctt
     4681 gacggggccg gcccctcgag ggaaacgggc gctgtaaacg atactgctga agaggaggcc
     4741 ggtgtggagg tgccgcctcc cggagacgtc gaggaagggg cggaagatct ggcgcctgag
     4801 ggatccgagg tgggtggaga ggatccggca cctgaaggat ccgaggcggg tggagaggat
     4861 ctggcacctg agggatccga ggtgggtgcg gaggatctgg cacctgaggg atctgaggcg
     4921 ggtggagagg atctggcacc tgagggatct gaggcgggtg gagaggatct ggcgcccgag
     4981 ggatctgagg cgggtggaga ggatctggcg cccgagggat ccgaggaggt tgcagaggat
     5041 ctcgtgcctc aagagccggt cgacgaggcg ctgatgatgg atgcgccgat ggaagatgcc
     5101 cccagggacg acgtccctct cccagaggct gccctggcgg cggccgtctc cgatctgaac
     5161 gcttcggagg agaacgagac gatggaggag gagatggtcg aggagaacga gacggaggag
     5221 gaagaggtgg aggaggagct ctcagagacc gataggatct ggcgcgaggg gaataacccg
     5281 gccacctaca cctggacccc ccaaaccttc tcggggttct tctacgacct cgacgacgac
     5341 gtggggactg agaccctcac cgttcacctg agggagtcgg ggggaagtta cgaccggtcc
     5401 atccgagagg atgagctgga gtacgtgacc cgcgccgatt ccataaccta tgagttcagc
     5461 gattggggcc agtatcaggt gataggcttc atggctcaga agtacttcgc cggatatgag
     5521 gggacggact ccgccgtggt gagcaagggg atcagcctcc tggacgagga ggagctccac
     5581 gaggttctga tcgacgacga tcaggattac accgtcgaca ccggctcggt cctccccctg
     5641 ggcgagggct acgagctccg gatcaaggag atcgacctcg acgggaacaa ggtctggctc
     5701 gccctcgcca aggacggctc ggaggtggac agccgagtcg tcgaccccac cagcctcgga
     5761 tcttcgacct acaagtacga ggccgacctc cggggcgata agttccctct ggtgatggcc
     5821 cacgtcggaa gcatcttcag aggggctgag acgagcctcg tcaccatcga cggcctcttc
     5881 cagatctccg acaagttcgt caccgtcgcg agcggcgaca cctacgggag catggaggtc
     5941 gactcgatat ccggaaacac catcatgatg gccaacgaca ggaccatcac cctccgaatg
     6001 gggaggaccg tcccggtgat gggggatgtc agcttcctgg tggccgacgc cagcgagctc
     6061 cggttcgctc cgacggtgaa gaggacgggg agctacgaga tccgggggac tatcgtcgac
     6121 ccggcggagg gtgagttcac ctggaccccc tacaacttcg aggggttcta c
//
LOCUS       DASM01000027           18688 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_31468, whole genome shotgun sequence.
ACCESSION   DASM01000027 DASM01000000
VERSION     DASM01000027.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 18688)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18688)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18688
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_31468"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    18362..18397
                     /estimated_length=36
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3821 a         5558 c         5581 g         3692 t
ORIGIN      
        1 cgcgagggcg tatccgttgc tcatccctcc aagttcatcc tggtcggaac catgaacccg
       61 gaggaaggag agatccggcc tcaactgctg gacagattcg gactgcaggt gagcgttgta
      121 ggcatagagg atgtagatca acggatgcag atcgccaaga tggcagaagc gtttgacgcc
      181 gatcccgatg gatttactga aaagagccgg gaaaaccagg cgcatctgaa ggaaaagata
      241 tccttcgccc gcgagatcct tcgaaacgtg agcttgaatg atgacctcct ccgtttaatc
      301 gcttccacct gcatcgatct tggagtcaag acgcaccgag cggagatagt cattaccaga
      361 accgccaaga ccatcgccgc cttcgatggt cgaaccgaag tcaaccagga ggatgtcaag
      421 aaagccatgg agctggccct ggcccacagg atgaggagca ggccctttga gccgccgacc
      481 ctcaacaaag agcggctgga gaagtcgatg gagaagaatc aggatcctca gaagcagcac
      541 cccaaggatc aggagaagag agaggagaag cgcgaggaga ggcaggagaa tgaggactcc
      601 gacggttcgc aggcccctct tcaggctcag gagaaggtct tcgagatcgg cgatccgatc
      661 gacgtccgcc agatcgacgt gcaccgacga agggacagga cggcgcgccg gaagaccgga
      721 ggcaggagga tgaacaccct ggcctcgtcc ggatccggaa ggtacctccg ctccaggatg
      781 cctgaagagg ggagggatat cgccatagat gccaccatca gggcggctgc tccctatcag
      841 cgattcaggg atggagataa cgccatcaag gtccaggacc aggacatcag ggagaaggag
      901 cgggtgagga agacatcggc agtggtaatg ttcgtggtcg acgccagcgg atccatgggc
      961 gctgtgagga ggatggagag cgccaagggg gccgtcctat cgatgctgat ggactcgtac
     1021 cagaagaggg acaagatcgg catggtggcg ttcaagggaa atgacgcgga gcttctgctg
     1081 ccaccatgct ccagcgtcga ccaggcttta aacaggctca gcgagcttcc gacgggcggc
     1141 aggaccccct tatctgcggg cctggccaag ggactgcagc tcctcaaaag cgagatgacg
     1201 aaggacaggg acgcgaaact gatgatggtc ttgatatccg acggaagggc caacgtatct
     1261 ttggggggga agatccgcga ggagatcaaa gagatctcgg agaggacgaa ggaggccggc
     1321 gtccacacta tcgtcatcga taccgaggtg gtggagtcgt ccttcatgga tatgaggctc
     1381 ggttactgca gagacgtggc ggatgccgct ggcggaaggt actatcccat atcggggctc
     1441 agctcggagg cgctcattag catagtcgaa ggggaggagc agagcctctt cggtatgaac
     1501 gcgtgagcat gctggaggtg catcctggga gagtcggctt ctgcgggccg ggagccgaca
     1561 ttcaaatttt agggctgctc cgacgaatcg gcgcccaatt ttcggtgaga tttgtaaatt
     1621 agatcttatt ttataacata ttatctgaca aagttataaa tagagctctt tagcatacga
     1681 gtttggtgtt atgtaacacc caagcaactg ccatctctcc cagcgtagct ttcgggcatt
     1741 ctgctgggag ggctgccatg gattgggcag gtgataaaat gagacgaact accaaggtcc
     1801 aaatcctctt gctgtcggca ttatgcatct tgctgatatc gatgccggcg atagcttccg
     1861 gttacaagct gggaatcttt ggaaacgcca acatggacga caccatcgac gaggatgata
     1921 tagcgtatgt ggagggcata atcaggggcg aaaatgaact cactgagctt gccgacgcca
     1981 attacgatgg caagatcgat gaggaggaca taactcagat cgagatgatc atccaagggg
     2041 agcctgaaga actaactctg ctccaataca tcggatatcc ggaggagttg accgaagagc
     2101 cggtcaccat ccccatgcct atagagagga tagttgccgt cggcggtacc tacggaccgg
     2161 agaccttatg cgcctttggt gaaggggaca ggatagtagg tgttcttgaa tccgctaaga
     2221 aactgggggt gctagagccc ttcctcaagg ataagccagc ggtcggaacc agcactttca
     2281 cctgggatct tgagaagatc ttagagcaag atcctgatat cgtattagct tacgccttta
     2341 gatacttccc ggagtacgac gatcagctac aggcctctgg tatacccctg gttcagatgg
     2401 actttcacga tcctgaaaaa ttttcacagg agataagaaa tctggggtgg atgttaggcg
     2461 atcaggaaag agctgaagag ctgatcgagt ttgaggagca gcacctggac ctcataaagg
     2521 agagaactgg cgaggttgaa cagagaccaa aagtatatgc tgagtggtat gcagactatc
     2581 aagcagttgg tccggataac gccaacaacg acgccatcac catcggagga ggagtcaata
     2641 tattccatga tgcttctacc aaatatccag aaattgactc tgagacggtg atcgttagga
     2701 accctcaagt catcataaag tttagcgata gccgggatat cttcggatac ggggttacgg
     2761 acactaagcc attggaggaa ctaaggggag ggatagcgaa ccgacccggc tgggagaata
     2821 ttgatgcagt tactaacgat aggatttaca tcataagcac ctacagtcac tcagttcaca
     2881 caagcgtgtt atattcgtac gttgccaagt gcatacatcc agaattgttc gaagatatgg
     2941 atccggatga gatctacggg gagtggtttc agaagttcct tggattggaa ttcgctggcg
     3001 tctacgctta tccgccacct tcgagttggt gattgaagca actgaggatg gtacggcaac
     3061 atcggggggg aggaacgctc cgtctcgggc ggcgtgcgct gccgagcggc cccaatcccc
     3121 attgttccgg gacgtttgat aaccagccta tccccaggag gcccgatcag tttccatcga
     3181 tagaagtttg atgaaaaata agtttgaaga tatgaaataa aaaaaaccat ctcttctttc
     3241 gatctttgaa tgatcactta cttattttcc tggtacatat ttattatctc gttcatggac
     3301 gccacggcga tgggcgtccc accccgggtt cccaccaccg agatggatgg cacatccgtt
     3361 cttctgagcc tctctttgct ctcggcggcg ttcacaaagc ccacggggac gccgatgacg
     3421 aggtcgggct tcgcctcacc accttcgatc agatcacaga gggtgagcag cgccgaggga
     3481 gcatttccta tcagaacgat ggacccgtcg agctcgtcct tcagcttcat aacgccggcg
     3541 gaggccctgg tgatgccttc cctttctgcg atatcgtcgc ctttccccag aaagatcgtc
     3601 actttgcttt tatgcccctt tttaaccacg cccgcctcca ccatttttat atctgcgtag
     3661 atccgggcgc ccctctccag ggcttcgagc ccggcgttca aggggtcatt cttgaacctc
     3721 aaaatatccg ccaagtccac gtcgcccgtc gctatcacac agcgctgtct gatccgatct
     3781 tctggggtat cgtttcccac aacgctggct atgtaacttc tgcttctgtt gtagatctcc
     3841 tgggcctcgc gggtgacaga tcccaggtct ctgtatacgt tctcgatcat atctcaacaa
     3901 cctctcggat ttactaatgc tcttacacag aatatcggcc ccttctatat gctggccctc
     3961 ttcaggacca tatgacatgc ggcggagaag gcggcccaga atcctatcat gaccagaaat
     4021 gatacccagg ggaagggctg gccgaggatg gcagccctga ggcagaggct cgaatgggtg
     4081 agggggaaga cgtacagacc gaacttcagg gcctcgggga cctggctcag ggagaagaag
     4141 gtccccccaa gaaaggtcat cggcagaaga atcaggctgc tgaaggtgcc catatcctcg
     4201 tgagacttgg ataggagggc cgccaacacc cccaaaaagg agaagagaag gcaggtgact
     4261 aggaggctgg ccaggaagag cggcgtcacc tggaaggtcg gcgatatcaa aagcgccagc
     4321 cccacgaagg cgacagagct caccagcccc cggactgcgc ccaccatcgc cttgcccagg
     4381 aggagggaag aggagcttat gggggccatg agacattcgt caaagctctt gaagtagagc
     4441 cgatcgacgt tgagcctcgt acccgccccg gtgaagctgc tgttcatggc ggtgagggcg
     4501 attatgccgg gaaccacgaa ggcgaagtag ctggtgccgt caactgagat gccccgcccg
     4561 agcccccacc caaaggcgac tagatagagg agagggctga ccagggtggt gatcaggtac
     4621 cggaatatcc ggcgcttgag gaccgcaagg tcggcccaga ggaccgagta ggcgtccgac
     4681 aagctcatcc agtcaccttc ctgcccgtaa gctcgatgaa gacgtcttcc agattggact
     4741 ccctcatgac gatgttgccc ccggatggca gcgtcttgac gtactctgta gcggcagccc
     4801 tgtccgggaa atatcgatac tccgtattgt tctcgctctc tctggactcc acggtgacga
     4861 ggccgaggtt ctggcgcagc tccaacgggc ttccgaggga tatcaatcgg cccttatcaa
     4921 ttatccccac ccgatcgcag agagcctcgg cctcctcgat gtagtgggtc gtcaggaaga
     4981 cggtggttcc ctcccatttc atccccctga ccagatccca gatcttcctt cgggcctgag
     5041 catccagccc tacggtcggc tcgtccaaga agagcagctt gggcttgtgg agaagagcgc
     5101 gggcgatcat agctcgcctg gtcatccctc ccgatatccg ctcgataaga tgatcgccga
     5161 actcctcgag gcccacataa tcgagcatct catcgatccg atctctccgc tcctttttct
     5221 cgatatgatg aagccgagca tgaagctcca ggttctccct caccgtcagc tcccggttga
     5281 ggctcatgtg ctgctgaact atcccaaact cctgcttcac ctttgagggc tcttccatca
     5341 cgtcgaaccc gtttatccat gctcggccgg aggagggctt ggtgagggtt gagagtattc
     5401 tgatggtggt ggtctttccg gccccgttgg ggcccagaaa cccgaaggct tcccctcttc
     5461 tcaccgagag gtcaaggccg tcgacggccc gcttctcccc gtactccttg gcgagatctc
     5521 tcatctcgat catcaaatcc atagtttatt tcagctccgt attccggggc tcagctcacc
     5581 gttcccgatc agggtcctcg gccatccaga aacggataga ccgccgcggc gaactcgtca
     5641 gctcatttcg atccgggatt atacgtttcc atcttaataa cttacggtta gttcatacgt
     5701 catgtgatat gagatgaaga acttctactc attatttctc caggtcccag gatcgcgacc
     5761 gacatgggat tgattcaagg gactaaaacc accagcataa ccaatacgat aagcctaacc
     5821 aaaacgctga ttaccgtcga agtgacaacc aaggagaggc ccaacctcat gccgaagacg
     5881 gaggtgtaat tggggatcaa agacctcacc gagtaggcag gaagcatgaa gaggcttccc
     5941 agcatcaaga ccatcaacgc cttcatctct gatatggccc cggtctgaat catcggcccc
     6001 agaagtgtta ttcccgcctt ggggctggcg acgtagatcg tcaggggcac caccgtctct
     6061 gacggtatcc gaaacgtttg agctatggga aggacgttga tcgatccgag gatcccctct
     6121 ctgctcaaat aaaggaccag gaacgtcatc acaaagaata gcgatgagat tcgcagaaat
     6181 atcctcatct gccctctcag agattttttt gcggcttcaa taatccccat cctatcctga
     6241 agctccctcg ccaccggaga gatcgacgat tcctccgata ggtatgccct tcccaagatc
     6301 actaccaata atagttttat caaccccgtt gctatggcca ccgctgcgta caaccctccc
     6361 acgatcatcc ccagaacggg tattacaaag gccagctgat atgtgaagaa ctctcggaaa
     6421 taaacgggaa cgctattcat aactgcgctg agaaaagcct ggcgctcgct caaagttccc
     6481 tcctctctca acctggcgat catggtgttt gccgcaaggg cggatccgaa gctgatcgcg
     6541 aaggtggagg cggataccga tggcaagcga gatatagaaa cgagaggcct ggcaatgtat
     6601 gagacccgtt gaaagaaccc catctccacc attacgcccg tcagaaagag cattacaaac
     6661 actatggaca tggtggagac ggtggagttc agcgtttcaa ggaggagatc gagcggactc
     6721 aattcatcat ctctatttcg ggaactcatg gcgagatcga tacagctcta ttcttagtta
     6781 tgtgacgtcg cacgccatgg attccctgtt tatggtatca gtagcagctc gcccagtaag
     6841 aggagttgca tagcgaccta ccaccggttt gtttataata atttcggttg aagtattact
     6901 atttgaatca taaacgatcg gatcttcgaa ggcgtgtccg ggtcgaacgg cgcctatctg
     6961 agatggaagg ggagactgaa ggtcgagata gactatctgt taatcgaaga ccttatgacc
     7021 gatgaccaga ggtaattccg ggtcagaggt ttagatgcgg cagatgaact tgcagagaag
     7081 cgatctcttg ggagtggtgg tgggcggcgg cattggcggc gcagagctgg tgcgaaacgc
     7141 caccaccaag gaggggctgg agctggtcct cgtcgagccg aatgatagga tcgagtgtca
     7201 ggccctctac cccgactacc tcggcggccg aatatgttca gactctttct caggatttcg
     7261 gaaatatagt tatacgagag acgaacctgg tggacgtctt catcgagctg accggccgaa
     7321 aggtagtggg tgggtaagat ggacttccac accatcctct gggccgacct gatgatcatg
     7381 aggcgaagga tgacgaggta cctcgtcacg acactattca acccaattct ctacctcttc
     7441 gccttcggct ggggcctcgg gagagatata agcattgaag gggctagcta tctagacttc
     7501 gtgattccag gaatcatagc tctcacggac atgaacggca gtttcgtcgg ggccggggcg
     7561 aggctgaacg tcgatcggct atcctttaga agcttcgacg agtttctcat ggtgccgatc
     7621 agcactttgt cgctagtatt cggcaaggcg atgatcgggg tatttcgggg cctggtcagc
     7681 tccattgcct ttctggccgt tgccctcctt ctgtccccca ccttcagcct aggtcccctt
     7741 tacgtcgtga gcctagttct aacgtgcctc tagttctcat ttttgggggg tgctagcggc
     7801 ccttctggcc aagtctcatg aagacatgaa caccttcagc agccttattc ttctgccgat
     7861 gaccttcctt gccggaactt tcttctccct atcccaggtt cccggccccc tgaagctcgg
     7921 actctacgcc ctgcccctga cccattcgag cctctgcctt cgggcgacag cgctggacca
     7981 gcccttcccc tggacgtctt tttttgccat atgcctcttt gccgcccttt tcctggccgc
     8041 atctcttgtg actctgagac ggatcagcga ataattgaga gttgcataga aacatttttt
     8101 tacttttgta agattggctt tttcgcgatc ttcctatttc gcagcctctt gataatgata
     8161 agctggttca acatacaaac atccgatgat gaaatctaaa aaattctttg cgctgccgcc
     8221 agaagatggg cagcgcacaa cattttatgg atgggatggg actcccatct cggtctgccc
     8281 attcttgttc agatcgcggg gtagacgaag aagttctcct ccgggtactc cagacccctc
     8341 atctccaggt actcctggta gacccccacc ggatccagct ccgcttcggg atgaatcatc
     8401 tcggcccagt aggtcaggcc cacaaccgca cccgtcccca gggttatgtc cctgaagatg
     8461 acgtagaccc tgccgtcctc cgcggcgggg atggtgtcgg ctccggggcg gctctcgatc
     8521 tcgttgacga aggcctccat ctcggtggtg ttggtccagc cgagctggtt tatcgccgcc
     8581 gggaccttga ttatcacctc gggcatcagg gcgaggaccg tctcccattc caccttgggg
     8641 ttcgcctcat ccgcgacgat gacgttctct ccgccggcaa gggagatcat ctcggagggg
     8701 gcagatccct ggccgtaggt ccagagagcc cccaggtccg aggcggaccc gccctcgacg
     8761 tagacccggg gcctaccgag ccctgagacc gcctcgacga cggaggcctt cttctcgctg
     8821 acccagaggt tgtaggcctc ggcttcagac tcccggtcca ggacctctcc gaggacgccc
     8881 atctcctcct ccagggtctc gggtatgtag aggtcgaggc ccaggacatc gatctccttg
     8941 aagggggcga ggttctcatc cacctcagca gccttgctgg catagcagat caccagcatc
     9001 ccggccccca tctccccatc ctcctccctt ccgaggtcgg ctatcttctc gtagttgaac
     9061 tccttccagg tccccaccgt ctcccagtcg cgagagaact cagtgaagta gtgggccttg
     9121 taggagatgt ccaccgaccc cacgatcttc tcgacctccc ctaggatgct gacggcatcg
     9181 gcagcgtcgg agttgaggac gacgatcttc tcgacggggc cttctatcgt tacgaatctc
     9241 cccgccgagt cggtgacggt caggggataa tcgacggcta tcgccatccc cgaggtcaaa
     9301 aagacgctca gggttaaaag caagatcaaa ggcattttct tcaaaaccaa gccacctttc
     9361 agctttttaa tgtagatctc gaagatcttt attaactaat aagaatttcg gtcgttaatt
     9421 tgtaattagt ggcggagacc gaaagccgga ttaattggtc ccgcccatat ccagcagccc
     9481 tggagcttat accctcggct ataacctaaa gatggggatc tcgatggact gggaagtcgt
     9541 aggttacgcc gctgcagccc tgacgatgtt cggctttctg ccgcagctct tgaagatggt
     9601 gaggacgaag tccgtggacg acgtaagcct cctcatgatg gtccagatgg gtctgggggt
     9661 ctctctctgg ctcgcctacg gcctccacct cgaaaactcc cccctgatcg tggcgaactt
     9721 cgtatcccta tcgatcctgg ccctcggcct cgtcgtctac ttcaggtaca ccaccaatag
     9781 atacctcacc agaagatact tcagataaga agaagaactg gccttgaggc ggggtgaggc
     9841 ttctcaccat cgccggccat tgagaggagg atttccatga tggagaggct gacgaagttc
     9901 cagaacgccg ttctgatctt gctcacctgg atgatggccc tggtggtctt tctcgcgacg
     9961 gcagagctct tttacatcgt cacaaaggag atcgtctccc ctcctctcat cctcctgggg
    10021 attgaccagc tcctggagat cttcgggtac ttcctcctgg ttctgatcgg gatagagctc
    10081 ctcgagacct tcaggatata cctcgctgag aaggtcatca acgtccaggt cgtccttctg
    10141 gtggcgatca tcgccatcgc gaggaaggtg ataatcctgg acgtcacgaa gcttccgagc
    10201 atcaccctga tcgggatagc ggcgatcgtc gtctccctct ctgcgggcta ctacctggtg
    10261 aagaagagcc agcaggacga gaaggcgtgc aggctgtagt cgggctgcca tgccggatcg
    10321 aggctttact accaccgtcg gatccaaccc ggtgcgaacc ctttaatccg atcggggcgt
    10381 aactctcccc aaggaggaga gaggacatga tccccaaaga taattggacc gatccgcccg
    10441 agagggtcag ggccgccctg gaggaggtgg cggacggcta ctctgacctg gcctccgacc
    10501 tccgggccga cctggcgggt gcggaaggca cagaggcagt tttagagctg gcggagaggt
    10561 cggccctgga gatgctggag aggcttcctc tgagccccgt ctactgcccc ttctgcacca
    10621 tccaccggcc cggggccgtc gactgccagg ggtgcggata cgccgccagg tacggccgat
    10681 gcaccgaggc ggggaccccc tacaaccggg cgatcatggc ccactacgac ctggtggagg
    10741 cggtggaaga cctccggggg gggcggcaga tatcggcccc ctcgataccg aaagaggagc
    10801 tccgggccgc cctgggagat caggcggacc gggtgaagga tctcgcaggg aggtttcaga
    10861 ggagggtcag aagggccagg tcgtcggagg agctgatggc cctaaagacg gagttcatgg
    10921 ccgatctcgt ggggtctctc cccatcgata gggtctccgg cctctgcggc ttcgacgacc
    10981 gccccctctt ggaggcgaag aggggggccc tccaggggct gaggcgccac tggcccgcag
    11041 cctgaagccg ccatgatccg gcaggttcgg cccccgtcga ggccgccctc ccgccccttc
    11101 aggaccgctt gcttccgccc agagctgagg cggcgaggat cgccaggggt atcagcccga
    11161 cgatcaggac cgccccgggg gagaagccca gggagtaggc gaggatcacc aggaggatcc
    11221 ccagaaggac cacggtccag gagcggcggg gccccagaga gcaggcgcag gcggggcttg
    11281 ggggctggga ctggtttttc ttctcctcga agcttctttc ttcaggattc atttctccat
    11341 caccatccat cagatagatc tatgaggaga tatctcttcc cggcgccgtc cccctctggg
    11401 ggaggcgcgc cccgcttggt caccttcgtc cctcttctcg gtggtctatc cagaggctga
    11461 tcagcctgtc cgacctcgcc agggcctcca ccgtcccgga gaagtggtcg gcggcggagt
    11521 tcatcgcctc catgaagccg gagatgtcgc catcgtcggc gagcttcgag agccgttcgc
    11581 attcgtggat gtacgcctcc ctcacccgtc tcgcgtcggg gttctcctgg atactggcgt
    11641 agagctcggg gctctgggcg aggatccgtc caacgaagtc gatcatgatc tcgtagacgg
    11701 ggctcatgaa cctccgagag cgggcgacgt cgaactccag ggacctgagg gtcgagccga
    11761 tggagatgta ggcgaagtgg gtgagggcct ggacgacggc catgatccta tcgtgctcct
    11821 cggcctctag gatctccacc cgggcgcccc cctcccggaa gatctcctcc atcggcgaga
    11881 gccagaagtc gcaccttccc gccgcgggga cgaggatcac cgtctgcccc cggagggtcg
    11941 gtatggtggg gccgaacatg gggtgggtcc ccaaaacctc cacctcctgg ggagcccacc
    12001 ggagcatcgc ctccatcggc cccctcttga gggaggtgac gtccatcagg aggctccccg
    12061 gcttcatccg gggggctatc tcttcgatca cccttgccgt ctcctggatg gggacgctga
    12121 gaaggacgac gtcgctcacc gccacctccg ccgggaggtc ggaggcgaag ctcaccccga
    12181 gcctctcggc gacctcgact ttgccgctcg gcccccatac ggagacgaga aagccttggc
    12241 ccttgaagaa gcgggcaaac cagcgtccag taccgccggt gccgccgacg atcagcattc
    12301 tcttcttcgt ctcctccgct ttatcatcga tctggttcat cggttcctca gctcctccct
    12361 aagggcttcc tccatgacgt ctattggggc ttttctgccg gtccagatct cgatcgagag
    12421 cgccccctga tgaacgagca tcggcacccc gtcgatgatc cgggctccgg cccgggccgc
    12481 gtcctgcaag agctcggtct cccggttgta gacgatgtca aagacgatct gcccctcccg
    12541 gaatagtcgg ccgtcgagga gcctggggtc gccctctctc atgccgatgg aggtggcgtt
    12601 tatgaccacc tctgccccgg ggatgagcct gtccagttcg gagaggccgt agcccctcca
    12661 ccccacggcg gccgccaggt cgagggcacg ctcccggtcc cggttggcga tggtgagctc
    12721 ggcccccgag agggcgagct ggcgggcgat cgcctttgca gccccgccgg cgccgatgat
    12781 gaggaccctt ctgccggcga cccggacccc agccccctcc agggcccgca gggcacccag
    12841 accatcggtg ttgtaccccc agatcccatc gccgaaggcg acggtgttga cggcccccat
    12901 cgccgcggcg ttctcgtccg gctcgacgat ggaaagggcc gcctccttga gggggatcgt
    12961 caggttgagg ccgccaaagc ccatcgcttc cgcccccaat atcgcgtcct tgagccgctc
    13021 ttttttgacg cggaaggcga ggtagcgggc cgagagaccg agggcggaga aggcggcatt
    13081 ctgcatcgcc ggggataggc tgtgctccac ggggtcaccg aagacggcgt agaccaacat
    13141 cgggggtaag ttgatcttta tgggtaaata agggtaggtc tggcaagggg agaagaaacc
    13201 ttacctctgg gccatcgggc ccccgggcaa acctctaaat ccaaatcgcc catacaaata
    13261 taaaactgat ctgttatacc tggtcaatct tctaaggatg aggagggctc tttatgaaac
    13321 taaaaacgat cttcttgggg ctggaggcgg gaggcagatc ggtcgtcatc atgaacggag
    13381 acgacgccga gcggctgggg gtcctggccc tggggagggt gaccgtccgg ctcgacggaa
    13441 tggtgaagac cgccatcgtc aacacctcta cggagatgat acagccgggg aagatcggag
    13501 tctgcaagcg ggtctgggag gggctcggcc tcgccgacgg ctccgaggtc gaggtggagg
    13561 tggcgccctt ccccgactcc atctacttca ttagaaacaa gctggacggg aggaagctcg
    13621 ccttcgacga ggtcctggag atcgtcgagg atacggtggc ggagaggctg agcgacgtcg
    13681 agatcagcgc cttcgtcacc gccctccaca gcttcggcct cgacctcgac gaggcgacga
    13741 acctctccct cgccatgatc aagacgggga ggacgatgaa gctagacgga gggccgatag
    13801 tcgacaagca ctccataggt ggggtccctg gagacaagac gaccctcctc gtcgtacccc
    13861 tcgtggcggc agcggggctc ttgataccga aatcctcttc ccgggccatc acctccccgg
    13921 cggggacggc ggaccgggcg gaggtcctga tgccggtggg cctcgacctc gaggagatgc
    13981 ggtcggtagt ggaggaggcc ggaggctgca tcgtctgggg gggctccgtc cacctggcgc
    14041 cggccgacga catcttcgtc agggtcgagt accccctctc catcgacccc ctcctcttgc
    14101 cgtcgatcat ctccaagaag agggccgtcg gcgccgacct cctcgtcgtc gacatcccca
    14161 ccggcagggg ggccaaggtg aagacgatcg gggaggcgaa cctcctggcg aaggacttca
    14221 tggagatcgg aagccgcctg gggatcaagg tccagggcgc cgtcacctac ggcgagcagc
    14281 ccatcggccg cgccatagga ccagccctgg aggcgaaaga ggcgatggag gttctcatga
    14341 accgacctgg acctcgggac gtggcggaga aggcgacggc catcgccggg atcctgctgg
    14401 agatggcagg gagagaagac ggcaaggccc tggcggagga gctcctccgg tccgggaagg
    14461 cggagcagaa gatgagggag atcatcgagg cccagggggg agatccggag gtcctgccgg
    14521 aagatatagc cgtcggagac caccagttca ccgtccgatc cgataggtcc ggccacctcc
    14581 tctggataaa caactcgtcg atcgccgagg tggcgaggtt tgcagggtgc cccagggacg
    14641 agggagcagg gatactcctc gcgaggaaga tcggggatgc cgtcgaggag ggggatcccc
    14701 tcttcaccat cttcgccgag aggggcaaca acctccagcg agccctggat aggctggagg
    14761 ggctgagggt ggtgggcgtc ggagatggga tggcgatgct gatccacgag gtgaaggaga
    14821 ggccggtggc gaagaggagc ttcaccctgg atagatgagc ccgccaggtt ctccggtggt
    14881 agaggggatg gtccccttca ccgccacctt caataatcac ctcctaggcc agctcctggc
    14941 gacgatcagc cccctcgccg cggctttccg cccgacatcc tggcgacagc cggtgcaaag
    15001 attcatccac gaggcggcag agcccctcgc ttgaaccggc gcggggatcc gatcctggat
    15061 gtactccatc tcctttttgg tggcgaaggg ccttccgcag atgcggcagc gggccatctc
    15121 gaaggtgagg gcgaagctct cttctccggg cgattcgtcg gccagtccga aggagatcgc
    15181 ccccgtcggg catgcctccg ccagcgcctc ggcgatattc gcctcgaact cctcgataga
    15241 cctcggaaat ctgatcgtcc gcttcgcccc tcgatccgag aggccgactg ccgcggatat
    15301 ctccggccag aggtttgagc aggcccgaca tccgatgcac tcatcctcgt cgacgagaag
    15361 gacgcgccag gggccgtgga tcggccccac gcccgttttg gcatcgagat cggtcatgct
    15421 ctatccctcc cgacggaggc ggcataggat tgagctatcc tcgaggccgc cctcctcctc
    15481 agctcctccg tcgcctcaaa ccggtctccg aagatcaggg cctccgtagg acaggtgagg
    15541 acgcacggag ggacctccct ctcgggacat aggtcgcact tctggaccat ggaggcgggg
    15601 tcgagatcga cggccccgaa ggggcaggcg actacgcaga ggccgcatct tgtgcacttt
    15661 ccggcctcaa agccgacctc cgcctcctcc tgggttaagg cgccggatgg acagactagc
    15721 gcgcagggcg ccgcctcgca gtggtggcag gcgacgggga ccgaggcccg gtccttcacc
    15781 gcctccaccg ttatcctcgc ctccccgtgg accctctcgc aggccacctc gcaggcgcga
    15841 cagaagatgc acctttccag atcaacgtaa atctccaatc gatcagaccc ccttcatctt
    15901 gcctaccctg caggctgcga ccttgaactc cgggatcctc gccacggggt cgatgacatc
    15961 gctggtgagg gtgttcgtct cggggaagtg gaagggcatg aagaggaccc ccctctcctg
    16021 ccgggtcgtc acccgggccc tcgcccaggc ctccccccgg cgggtctcca cctccaccag
    16081 gtccccgtcg acgacgcccc actggtcggc gtccccgggg ttcacctcga cgaagagctc
    16141 cggagccctc ctcatcaggg aggggctcct cctcgtcatc gacccggcgt tgtagtggag
    16201 gacgatccgg ccggtggtga ggaggagggg atagtcgggg ctcctctcct cggcgggggg
    16261 ctggtagtcg acggggacga tcctcgccct cccctcgggg gcggagaact cctcctggtg
    16321 gaggatcggg gttcccggat ggtcgaggaa ggggcatggc cagatcaccc cgccccgggc
    16381 cccggcccgc tcccgggtga ttccagcgta gcttttgacg accctaccga tctccgccag
    16441 gacctcctcg gggccagagt aatcgaagtt gagccccaga aggtccgcga gacggatgat
    16501 cgtcgagagg tcggacctcg cctcccccgg tgggtcgatg gcccggggcg accactggac
    16561 ccgcctctcg gagctggtgt aagaccccgc cttctccgcc caggccgctg ccggtaggac
    16621 gagatcggcc atctcggcgg tctcggtcat gaatatatcc tggacggcca ggaagtcgag
    16681 gttcgcaagc tccctcctcc cccgggacga ggcggagtcg gagttgacga tgtcctcgcc
    16741 catcacgtag aggagccgga ggtctccgtc cccggcggcc accggcatgg cctcggcggt
    16801 catccccatc cccttcggca gggaggcccc gccccaggcc acctcgaagc ggcggatccc
    16861 ctcggcgtcg ctgacggata cggagccggg gtagaagtcg gcgaggaccc ccatgtcgca
    16921 ggccccctgg acgttgttct gcccccggag ggggtagagc cccgcccccg accttcccag
    16981 atggccacag atgagggcga ggtcggcgca ggcgctgacg ttatccgtcc cgtgggagtg
    17041 ctgggtgatc cccatggagt agaggatcgc cgaggcgggg gagctggcgt aggccctcgc
    17101 cgcccggagg atggaggagg cggagacccc ggtgatctct gccgtcctct ccggcgtata
    17161 ccaccggacc ttctccgcca gctcctcaaa gccgacggtc ctctcctcga cgaacttgct
    17221 gttgtagagc ccctcctcga tgatgacgtt catcatcccg ttgaggaggg cgacgtcagt
    17281 ccccagattg atctggaggt ggaggtcggc gagccaggcc gtgggggtta gcctggggtc
    17341 ggcgacgatg atgaaccccc cccggtcctt agccctcagg gcccaccggg cgacaagggg
    17401 gtggttctcg gcgaagtttg acccgatggc gaagatgcag ccggagttcg cgaggtcgga
    17461 tatggggttt gtcatgcagg cggcgccgaa ggcccggttg agggcgggga tcgtcggcga
    17521 gtggcacctt cgagcgcagc agtccacgtt cttcgaaccc aggaggcggg ccatcttctg
    17581 aaagaggtag ttctcctcgt tggtgcactt cgctgacccg agaaacccga gggctccggg
    17641 gccgtagtcc ctgatcgtct ttttaatctc cccggcgagc ctctcaagag cctcctccca
    17701 ggagatcctc ctccagccgt ccccggcctt caggaggggg aaccggagcc gttcggggtg
    17761 gccgagcacc tccagggccg cgttcccctt ggggcagagg gccccctcgt tggtggggtg
    17821 gtcgagcatg tactcgatcc cgggcccttc ctcccccttt cgaatgtaaa aaccgcaacc
    17881 cagggcgcag taagggcaga ttgtgggcga aagaccgtcg tcggccatga tgatcgtcac
    17941 ctctcctcaa ttaaaaaggc gtatggggcc ctcaccggcc gctgccggcg gccacccccc
    18001 tcgccagctt cagggcggac tcagccctct tcgactcgga gaactcctcc agctcctcga
    18061 agacgagggc ccccgtgggg caggcggaga cgcaggcggg gacctccagt tcggggcacc
    18121 ggtcgcactt gatcgccacc cgttcctccc ggttcctccc gatcatcccg tagctgcaga
    18181 ccgacgcgca ggtccagcat ccgatgcagg cctcggggtt gtgggtgacg accctggtca
    18241 cctcgtcctg gcggagggct cctgtaggac agacggagac gcaaggagcc tcctcgcagt
    18301 ggcggcaggc catggggacc gagatggcgt actcggggac gaactcgacg tagaccctct
    18361 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct ttcaggcctt atcaggatct
    18421 ctttcatctc ttcagctcct ggtgggggat cggggctagg cggagagccc cagccccttc
    18481 ctcttctcct cgatcttggc gatcatcaat ttagcggcct tgaaggggtc cggctccacg
    18541 gcgaaggagg cgcctacgac tccctccacc cccttcgtca ggacgctcgt cacctcgggg
    18601 ctaccgagga ccggcgggac cgtccccagg acggtataga ccccggaggc tatgacgtaa
    18661 gccccgatgc tcaccgcctt ctcgctca
//
LOCUS       DASM01000028           13283 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_937, whole genome shotgun sequence.
ACCESSION   DASM01000028 DASM01000000
VERSION     DASM01000028.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13283)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13283)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13283
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_937"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    11896..11939
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2565 a         4096 c         4191 g         2385 t
ORIGIN      
        1 acgcaggtcc agtggtagta tccgaggacg cctgtccccg tcacctcgtc gtactcctcc
       61 agcaggcgac cgcagatcgc gcacctctcc ccctcgggga cctcgatctc cgagtcgtcg
      121 cagatcacca ccacggccat gaccggatat agcgtctcca gttttaaagg ttttgccgcc
      181 gtatatccct tcttcccccc gggaggtctt tttttggggc gaaagggagg ggggtcgtcg
      241 gaggtccacg ccgcccacgg gcctcgaaga ttctgatccg aggccataga cgcctccggt
      301 cccgcctggg gggcgatcat cccggatcga tagatacata acggccttcg gggatggtat
      361 cccatagaac gaggtgtcca tcgttgaaaa taatcggtct tgaagaccag gaggtcgagg
      421 ccagcctccg gagcgctggc ctgggcctat cggttccaga ggcgcgctcc gtcgccgagg
      481 cgctggggag ggaccccacc atcccggagc tcttcaacta cgataccatg tggagcgagc
      541 actgctccta caagagcagc cgcgccaccc tgaacgagtt cctctgcacc tccggcccca
      601 acgtcgtcca gggtccggaa gaggacgccg ggatcgtggc catcgacgac gagtggtgcg
      661 tcgtcatcgc ccacgagagc cacaaccacc cgagccagat cctgcccaac gaaggcgccg
      721 ccaccgggat cggcggcata gtgagagacg tcaactgcat gggcgccaaa gtcgtcgcca
      781 ccgcagaccc cctccggttc ggcgacccct ttggcgggga taaaaaccac gtccgctggg
      841 tcgccgaggg ggtcgtcgac gggatctggc agtacggaaa cgccctgggg gtccccatca
      901 tggccggaga cgtcgtcttc gatgacagct tcgacaccaa ctgtctcgtc aacgtcgtcg
      961 ccatcggggt agtgcgacgg ggcgagatca tcaagagccg ggcccctgcg ggcgcgggag
     1021 agaaaggcta cgacgtcatc gtcgtcggaa agcccaccga ctccagcggc tttggcggcg
     1081 tcaccttcgc ctccgaagag ctccgggagg aggacgagga ggcgaaccgg ggggcgatcc
     1141 agatccccga ccccttcctc aagaacgtcc tcttcaaggc gaacgaggcc ttgttcaagc
     1201 tgacccggga ggaggggatc accatcggct tcaaggacct ggggggcggc ggattcacct
     1261 gcgcctcctc ggagatgggg gcgagcgccg ggtacgggat ggccctggac ctggacctgc
     1321 tccacaaggt gagcgacgac ctcccccccg aggtcgtcgc ctgcgccgag acccaggaga
     1381 ggtttatgct catctgcccg ccggagctga cggagcgggc cctcaagatc tacaacgagg
     1441 actgggacct cccgaacgtc tacgaggggg cgagggcctc cgtcgtcggg aaggtgacga
     1501 cgaacggcag gtacatcacc cgttaccggg gcgaggtgac ctgcgatgtc ccgatccagc
     1561 accttacggg ggggatccgc taccagaggg aggagcggcc ccggatgacg agcttcgagg
     1621 agccggaggt cgcccctcct gaggacctaa ccgacgccct cctgaaggtc ctggcatcgc
     1681 cgaacgtctc ctccagggag cacatctatc ggtactacga cacagaggtc caggggaact
     1741 cggtgatcag gcccggggag gcggacgccg gcctcatcgc ccccgtccgg ggcaagaagt
     1801 tcgggatcgc cctatcggtg gacgcaaacc ccttttacgg gaggatcagc ccctactggg
     1861 cgggggcgac ggccgtcgcc gaggcgatga ggaacgtcgc cgccatcggc gccacccccg
     1921 ccaccctcac cgactgcctc aacttcggaa accccgagaa gcccgaggcc ttctgggagt
     1981 tcagggagtg cgtccggggc ctctccgacg ccgcgaaggc cctctggctg aaggggtacg
     2041 aggatacccc cgtccccatc gtctccggga acgtcagctt ctacaacgag tcnnacgtca
     2101 gggacgccgt caccatggac ctgaagaggg caggtgactc gatctacctt ctcgggcccc
     2161 ggttcgacga gctggggggg tccgagtact atcggacggt ctggggagct gcgggcagga
     2221 atgtgccggt ggtcaggttc gacctggaga ggagcatgat ctacgccgtc atcgactcga
     2281 tagccgaaaa aaccgtcgtc gccgcccacg acatatccaa cggcggcctt gcggccaccg
     2341 ctgccgagat ggccctggtg gggaggggag acctgggcat cgatctgaac ctcgacgccg
     2401 ccggatcgga cctccccgcc gaccggctcg tattcagcga gtcctcgggc ttccttttgg
     2461 agggcaaggc cgggtccgag gaggatctgg agcggattct gaaaagatac ggcctcgaga
     2521 tcatccgcct cgggaaggtg accgactcgc cggagctggt catgacccac cgggggaaga
     2581 ccgtggtcag gatggacctg atggtggcga agaaggcctg gaccggaggc ctcggggagg
     2641 cgatgagatg aagatagccg tagtccagtt cccggggacg aactgcgagc acgagaccct
     2701 cgtcgccgcg aggagcgctg gcctttccgg ggagatcttc cggtggaaca ggccggcaga
     2761 ggagctatcc ggcttcgacg ggtacataat acccggcggc ttctcctatc aggacaggct
     2821 acgggccggg gctatcgcct cgaaggagcc ggtgatggac gccctccggg aggaggcgga
     2881 gaggggcaag ccgatcatcg ggatctgcaa cggctttcag atcctggtgg aggcggggct
     2941 tcttccgggc ctcggaggca tagacgccgc cctcgcctcg aacgtgatgg tctcgaaggg
     3001 ggagatcgtg aggcgcggat tctactgcgc ctggacgacg ctgcggcacg aggcgccgcc
     3061 gaagaggtgc agcgggagct tcctcctgga ggtgggggag ctcgtccgga tgcccctcgc
     3121 ccacgccgag gggaacctcg tcaccgtcga cccggacctg atggcgaggc tgaacgagga
     3181 ccaccaggtg gtattccggt actgcaatga ggcgggggag gtggaccctg agtatcccgt
     3241 caacatcaat gggagcctcg agaacgtcgc tgggatctgc aaccccgagg ggaacgtcct
     3301 ggggatgatg ccccaccccg agaggtcttc cttcgcctgg cagctcccgc cggctgatcc
     3361 ggcgaagagc gtcggggata tggaggcccc cggacccggg aggaggatat tcgagtcgat
     3421 gaagcggtac atagaggtga ggagatgaag ataaacctca gggtctggct gaagatcccc
     3481 gacgccgagg cggccaccgt caaaaacacc ctccgccgga ggatgggcta cgagggcgtt
     3541 cttacggacc tccgccggga gaggctctgg tcgatcgagg tcgacgacgg agtcgatgac
     3601 ccgatggacc ttgcgcggcg gctggccagg gatctcgtca acgagaacaa ggagagctat
     3661 cgggcctccg tcggcgccgt aaccttcgag ggagggtacg tccccgtcag ggtggggcta
     3721 cacatcgagg acggcgaggc ggtctccgcc ctgaagaggc tccgggaccg cctcggcttc
     3781 gagatggtga ggggggtgga gaggtccgtc gtctggaagc tggccctcag ctcccccaac
     3841 ctccggagga cggcggagga gatcgccgag gggctcctga taaaccccca caaggatttt
     3901 tatgagatca tgacgtgaag ggaggcgtat gacctcctac atccagcatc ctctgctgaa
     3961 ggagaaggcg gtggagaggc ggctcttcca gctggagctt gcggcctccg ccctccaggc
     4021 ctcgacccta gtggtcctgc cgacgggttt gggaaagacc gtcgtcgccc tgatggtcct
     4081 catcgccagg ctaaaccggg gcaaagtcct cttcctcgcc ccgacgaagc ccctggtgga
     4141 gcagcacgcc tcatttcttg agaacgtcct cgccgacagc accatcgtcg cctccttcac
     4201 cggtgagatt ttgccggagc ggcgaaagga gatgtgggag gaggctaaga tcgtcgtatc
     4261 gaccccccag gtgatcgaaa acgacctatt atcccggagg atatcgctcc gggacgtctc
     4321 ctgcatcatc ttcgacgagg cccaccgggc ggtcggaaac tacgcctatg tctatatagc
     4381 ggagcggtac ggtcgagagg gaaccgatcg gctagtcctc gggatcacgg cgagccccgg
     4441 gagcagcttc gagcggatcg aggaggtctg cggaaacatc ggggccacga ggatcgagac
     4501 caagaccgag gcggaccctg acgtctctcc cttcgtccac cagcgggaga tcgaggtcgt
     4561 caaggtggcg gtccctccgg agatccagag ggtccggtcc ctcctggagg aggttctgga
     4621 ggagcggggc gaggccctcg accgcctcgg cgggtccgat ggcctccgga tgccgaagag
     4681 gatggatcgg acctcgaaga aggagcttct agacctgcag aagaggctcc ggggccttat
     4741 tgcaaagaac ccgtcgccta gcctctacca ggcggtctcg gtcctggcgg aggtcctgaa
     4801 gctgaagcac gccgtcgagc tcgccgagac gcaaggtgtc gactccctcg cccggtacct
     4861 ggagaggctc gcctcggagg cggagtcgag gggggggagc aaggctgcga agaggattat
     4921 ggaagacccg agggtggagg aggcggtcgg ggatctcgcc aggatggacg tcgaccatcc
     4981 gaagctccgg gccgtcctgg agctggtgaa ggagcagctc accgccaagg ccgactcgag
     5041 gatcatggtc ttcaccaact accgggacac cgccagctcc gtcctggact tcctcaagga
     5101 ttcgggagac gagatcatca ggccggtccg gttcgttggc cagtccagcc gggtcgccga
     5161 cgaggggctg agccagaaga ggcaggccga gatcctccag aagttccggg agggcgccta
     5221 caacgtcctc atcgccacct ccgtcggcga ggaggggatc gacattccgg cgacggacct
     5281 ggtcctcttc tacgagccgg tcccaagcga gatcaggtcg atccagagga aggggaggac
     5341 gggccgggcc agagccggcc gggtcgtggt cctggtggcc agggggacga gggacgaggc
     5401 gtaccgctgg atcagcgatc gtaaggagaa gagcatgcgc cagcagatcc gggagatgag
     5461 ctggccggac cggcgggacc tgggatcggc ggccggggcc gggggaggag agggccatgg
     5521 cctctcctcc ggcccccggg taggaaggca gctcgccatc ggcgaggtgg ggccagggcc
     5581 ggggggcgag cccggagggc ccggcgagcc caggcccttg ggccccaccg acctcttcgg
     5641 cctctccgag gaggcctccg ccctcgtcat cgtcgacccg agggagagga gcctcgcccg
     5701 gatgctggag tcgatggggg tgggggtgac cctgcgggtc ctggaggtgg gagactacgt
     5761 cgtcagcgac cgggtcggga tcgagaggaa gaccgccgac gacttcgtcg actctctcgt
     5821 cggcgcggag aggaacatct tcgcccagct gaaggacctc gcccggtcct acgagaggcc
     5881 tatcctgatc ctggaggggc gagacctcta cgcccggcag gtccacccga acgccatccg
     5941 gggcgctctc gcctccatag ccgtcgactt cggggtcgcc ataatcccga cggccgacga
     6001 ggatgagacc gtctcggtga tagccgccat cgcccggcgc gagcagcagg cccggggcgg
     6061 cgccccacag gcccacggca agaagaccca ccggaccctc aaggagcagc aggagtacct
     6121 ggtctcctcc atccccggcg tcggaaatgc ggtggcgaag aacctcctcc gccacttcgg
     6181 gtccgtggag agggtcttcg aagcgaccga agaggagctg aaggaggtgg agctggtggg
     6241 gccgaagacc gcggaaagga tccgggagct ggtgggcggg gagtacaagg ggtgaagttc
     6301 gtccagataa ttgattgaat tattttatac tgtacccatg tacatagctg gtataagtcg
     6361 taagcgaagg cggtgaatac cattttaacg tgaactctcg ggaccgtcgt caccatgata
     6421 tgcgcgcttc ggaacactct tttaataaca gctaactgac gttcgcccgg aaatctcagt
     6481 ttgctaatca agcggtttcg cagctgatcg agatcgctta gaggatgacc cgctgctgct
     6541 cgcttcatgg tgaagtcgtt tcccttagcc ttcacaccga aatgtccttt gtccctgagg
     6601 accacctctc cttcaaccga tagatcaacc cgactatcgt gaaccgaagc aattgtcgat
     6661 tcgatgctcc ttatcaggcc gtaatcgata tcgactttag aatgaagttt atacccaaaa
     6721 tggagttcct cacccttctt agcccaagtt ccatccctgc tccgacgagt cctagcgttc
     6781 tctcttcggg gtttctttga acctccttga tcggcctcta taaacgtagc atcctgaatg
     6841 gttcctcttt ttacctgaag ccctttaaaa tcgagctgcc tctggagatc ttcccaaaca
     6901 gattcatcct ttcccgtctg ggccatccgc tcgcgaaaaa gccatatcgt cctggaatcg
     6961 ggtacggaat caggaactcc taagaatttc ataaaagaaa tccgatccga aatctgcttt
     7021 tcagtctcga ggtcgctcaa cccgtaccac tgctggagca aaagcacctt gagcatcagg
     7081 ataagatcaa agttcggcct tccgcctcat tcagttttgt tatgatacat ctattcaaga
     7141 attggtcgaa atggctccca ctcgatcaag ttcgttatct gtgaaagcct atcgcccagt
     7201 ttctctacgt tcttgtaagc ttctcgaagg ccccaggcgg taagagaact catgcaatat
     7261 ccatgcatct atctgtatat aattttttcc attgactagc tgggtttttg ggagtcctca
     7321 ttcgcatcta tggaaatagt agtgccagtc tcgatatcaa ccggcatcag accggctcca
     7381 gttccgggct ttccaccagt tcatcttcca gtccttgcga gggatcctga ccaggccgcc
     7441 cagccgatca acagcatccc tctttatctc tatatatacg acctcgccct ctctgagatt
     7501 gagcttctcc agaagcttca aaacgtcgtt ctcgtaaata gccttcactc ccattaggtc
     7561 caccctccac agtttcgatg tcttatgcta aaaacctgct gatctgaagg tagaacgcaa
     7621 atccacttcg atcaattctt gttaacccaa gaccctgaaa agcatattaa agcaggcgga
     7681 cctaaaggag atcgaagatg gacagaaaga aatatgcaat catcatcggg aagggcgagc
     7741 gaaatcgctc tgcttactgc ccggaccttc ccggcgcggc cgccgccggc gagacggaag
     7801 aggagacggc agagctgatg agagaagcga tagagctcca tctggaagcc tgaaagaaga
     7861 caacctcccc atacccgagt cgaccgccat cgccaggagc gtcgaaattt ctgtctgaga
     7921 cgtttgcctc cccaatgaaa gagatgaata tggttgaaat agcgtatcac gtccccgacg
     7981 aggaccttct ggccctgaag ctcgatcccg atgagctggg ccggaagatc cggatggcag
     8041 cggcggtcaa gctcttcgag atggggaggc tgtcgtcggg agccgccgcc agcctcgctg
     8101 gcatcccccg cactcttttc ctgtcgaagc tcgccgatta cggcatcgat cctttctttc
     8161 tggccgagga agagctgaag agagaggccc aacgcggctt tagccatcga aaagatttct
     8221 tccgctgata gaataaaatc gctatcaaga aaggtaaatt ggtgcggggg atgggattcg
     8281 aacccacgaa ctcctgcgag actaggccct gaacctagcg ccgttgacct ggcttggcga
     8341 cccccgcgat aaagaggcaa cgaccaagtc gcagtacacc tatttaactg tattcttgcg
     8401 tgcggctcgt tcgtagggtc ctcgatggcc ctctcctcgc ttcaacgagg atcccggaag
     8461 gaatggggga ggatttcggg gagggcctgg gggggaggcg gcttgctggc ccgccgacgg
     8521 aaggccaata cctttaacta tattcgggct ggagttggag gacgtgcgat cgatccagaa
     8581 gaccggaaca aaagaggctg gcggcaagct ggagcggagc aggacgtcgg gggcgaggta
     8641 cgtcctcctg aggccggcag gctatcctct caagagcgtc tttcaggaca accccgaggt
     8701 cgatgacccc cgcctcttcg agcgctacgc ccgggagcag tggtacgggg agctggtgag
     8761 gccgggctgc tacctcttcg accggaggct ctaccccgac ttcgccttca aggtgataaa
     8821 ggcgaacccc agggtctcgg tcgtcggatc ggagacgagg atcgtggtgg agaggaagaa
     8881 ggccgaggcg gcgaagatca agagcgaggt caccttcgac gacgtcgtcg gccaggagga
     8941 ggcgaggcgg aagatgaaga tcgtcgagag gttcctcgcc gaccccgaga tcttcggcaa
     9001 gtgggccccc aggaacatcc ttttctacgg cccctccggc accgggaaga cgatgatggc
     9061 caaggccctc tcccgggaga cgaaggtccc catgatcccg atcaaggcta cgaccctcat
     9121 cggcgagttc gtcggcgagg ggtcccgcca gatccacggc ctctaccaga gggcggagca
     9181 gatggccccc tgcatcatct tcatcgacga gctggacgcc atcgccctcg accggaggta
     9241 ccaggacctc cggggcgacg tctcggaggt ggtgaacgcc ctcctgacgg agatggacgg
     9301 gatccactcg aggaaggggg tctgcaccat cgccgccacc aacaagatcg agttcctgga
     9361 cgagtcgatc cggtccaggt tcgaggagga gatcaggttc ggcctgccga gcgccgagga
     9421 gaggctcacg atcctggcga ggaacgtcga gaccctcccg gtggaggtgg gcgccctgga
     9481 cctcgccgcc atctccaggc tcaccgaggg cttctccggc cgggacctcg tcgagaaggt
     9541 cctgaaggtg gccctccacg gcgccatcat cgacgaggtc accatcgagc agcgccacct
     9601 ggaggctgcg gcggcgaagg cgaagaagga gacgaaagag cctccggcgg agatgttctc
     9661 ctgaggggca tcgccccccc tacctctcgc ccgcctcttc gcctttcgag ggctccttct
     9721 ccctcccatt catctcctct tcagtcccgg tccatctacc ctgggggtgc catcggccgc
     9781 ccctcgcccg ggcggtggag gaaagggctt ttgtacagtt taggccaata aaggtcagaa
     9841 tggcgatgtt atgtggcggg tgattctggc gaagttcaag cgctatccgg cgcaggagaa
     9901 ggtggtgagg ctgatcctgg agaggggatt tcaggtgagc gaccggggga gggtcgtctc
     9961 gggggggatt gagatacccc acgcccagat cgcgaaggag ctggacgtcg accggagggt
    10021 ggtggatacc accgccgcgg cgatcgtcga ggacggcgag ctctggaaga tcttcaagaa
    10081 cgtccggtcg atgacctccc tcgccgaggt ggccccggtg atgggcctag gcgtcatcgt
    10141 gatcaccccc gtcgacgccg cccagaccgg cctcctcggc gaggtggcga gcgccgtcgc
    10201 gagatacggc ctctccataa ggcaggcggt ctccgacgac ccctacttcg tcgagcggcc
    10261 gaagctcacc atcatcaccg aggccaagat ccccggggag ctggtccggc tcctcatgga
    10321 cataaacggc gttcggagag tgacggtcta ctagaactgg cggccttttg gtgatgatga
    10381 atgaagatcg gcgtctgtgg ggtaagctgt gagatatctc ctcgaagggt caaaggcgtc
    10441 tgtcccaacg ggagtcaggc ttgcgttccc agggagaacc agttctgcaa aatcgcgacc
    10501 tgtgccttca ataaaaatgt gaggcattgc tttttttgtc cggagttccc ctgcgatacg
    10561 acgaagacgg ggcccataac tttcggcttc tgccagtaca tctcgggtta aacctcgacg
    10621 acggttccgg ctagctcctt ctggccggcc ccttccggct ggccccttct aatcgaagac
    10681 accccgccct cgacttactc ctctttcccg acagctcaaa gcccatatcc ctcaacagct
    10741 ccggggtcct cccccgaccg tggatcagcg cctccaccag gtccgagggc tcgtcgatgt
    10801 ccattgaaca ccggaacgac tcgaagaccc cgaccgaaag ccccagctct tccgcccttc
    10861 gaaggtgctt caagaaactg aggccgtagt agcagaccct aaaccgaggg tctctgatga
    10921 ggatcatatt tgttccgccc cccctccctg gagcgatcac cacgtcgccg ggggtgcgga
    10981 cgagatcggc gatatccgag gggctcatca gggggaggtc cgccatggcg atcaggaggt
    11041 cgccaggcca gcccttcctc tcctccgcct cgatgacgag gttgagggcc tcgtccagct
    11101 ccttcggaga atcgaggacc tcgatcccgg acctttttcc tgctccgctt atcttatcct
    11161 cgaggacccc acccccagga aggggactct tcagcaggat cgtgacggta cccaaacccg
    11221 agacggcgtc gacgacgtcc tcgagcatcg cgagggcgag cctctccctc tccacggtag
    11281 ataagaccgt tgagagcctg ctcttggcgc agtcgagctt gaagggtatg actactctta
    11341 tgggccgcat ggctgatcag acacttcgat ctagatcccg gaatcccgat cccggagccg
    11401 ggcccggatc acgtccagct cccgcggccg gaagagcctc gccccggcct tcgggagggc
    11461 gagcccccgg aggacggcgg cgggggttcc gtcccccgcc tcccccatca ggaggttcgc
    11521 catcccggcg acctcgtcgg cgatcgcctc cagggttatc tccagctccc tcccctccag
    11581 gtcccgctcc ccccgccagt cccgcagggc cgcgatcccg gcccagccga tggccacgcc
    11641 cgcgacgccg cagcggaagg gccttccgca ggtgtcggtg acgatgacgg cgcagtccct
    11701 accgaggcgg cggcggatct cctcggcgct ggctgtgggg tccaccggga ggagtaggat
    11761 cctcccctcg ccgacgttcg atctatctat gccggcgttg accccgatgt ggccgaacct
    11821 cgtcgccacc aggaggaagg ggtgatctaa aagaacctcc tccgcctcct ccaggaccgc
    11881 ctggacgaac ctcggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    11941 ggtggaggcg atgcagacga cgtcgccgtc ctggacggca aaccgctccc tgatcatcgc
    12001 tgcgaggtcg tccccctcct ttatcgcggg aaggcccctc accagatggc agcagaccgg
    12061 gtccatagag atcggctccg gggcggctag aagctacctg aaccgctcct cgtaaggcat
    12121 tctgctcagg tggtagagcc tcgcgtggac gaggaggtcg aggaggtagg agtagttctc
    12181 ggcgacgatg gcgagccttt cgtcgccccg ctcctcccaa ggtagagagt tgtagctatc
    12241 ggcgaactcc ttccagtacc ctggggggag gaactccagg aggtagaggt ggcggtcggt
    12301 gaaagtgagg tttatctccg gctccatggc agatagcccc cctcaggccg agcccttcct
    12361 ctccacgacc tctgagaagg cgagggttcc gctgatcttc tttatcttcg cccggacgat
    12421 gtcgcccttg gccgtccccg ggacgaagat catgaacttg tcgaccctgg cgattccgtc
    12481 gcccttcctc cccaccgagt cgatccgcag ctcgtaggtc tccccctcct ctatgacgtc
    12541 cttggggacg accgtcttcg tcttcctctt tcttacgggc cgatgagccc cgcaggcctc
    12601 gcacttgagc atcaggacgc gatcgctcct gatcagctgg gtgtcgggcc tgtggcactc
    12661 ggaacagatg acgtactcct ggacgtagga gtcgatgtgc cgctcgaggt tctgctccgt
    12721 gaaccttccc tggaatacgg cgtgctggcc ctcgatcttg ccggcggtcc ccatctcctg
    12781 gaggatgaac ttcatcaggt gatccgggtc ccggttgagg gcgtcagcga tgccggcgaa
    12841 gttctccagg accgtcgtct tcccctcgta aaagatcttc ggggggggta tgacgaacct
    12901 gtcctccgta cccctggagg cgggaagcct gctcatggcc cgctcgagcc ctttcatgta
    12961 gtcttccatc agaacaccga caacctcgat tattttatca gctcggcgcc cttctcgacg
    13021 acgatgtaga tcggagtggg cagcttatgt cccgccctcc tcagggccat cttcgcctgg
    13081 gggacgctcg tgggggcggt gctgacggag aagatcttct gttccgcatc caccctcgca
    13141 gcggtcccga cggccttccc aaaggcgtgg cgcatcccgc tggagactcg gtctgccccg
    13201 gcccccgtcg cctgcttgtt ctcccggagg acctggtggg ggtagaccct cagcttcatg
    13261 tggaagttga gccttccgac gta
//
LOCUS       DASM01000029            4730 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_169061, whole genome shotgun sequence.
ACCESSION   DASM01000029 DASM01000000
VERSION     DASM01000029.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_169061"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          933 a         1451 c         1448 g          898 t
ORIGIN      
        1 gaggtctcgg cgtcaattat gactctgcca ccaacttcgc ctccaggatg gacgctgccg
       61 gatggcacag acagtttctc tggggaaaca acgctgcctt cgagaaggac tggaaagacg
      121 tgacgaaagg aggatgggat gatagatacg ccgatgcagc cgatctcgct ttcttcgagg
      181 gtcacggatc tccaagcggg atatacttcc ccaatgactg ctgcgacgac aacaagtgct
      241 cgatagatga cgcccggtgg ggagacctag acttggaatg gataggggcg cactcatgct
      301 cggttcttgc ggactcaaac ctcgcgacct gggcctacac cgtatgcggg aacaacgggg
      361 ctcatatgat gtgcagccac aagaacaccg tctatgccag aaacgcaggt gaccgctggg
      421 ctcagctcat gttaagcgga tggaccgtca aaggtgcctg gttccagcag catatcgaaa
      481 agcagcccag cggcgtcaca gcaagggtta ttgcaaccca ggcgaccgcc aacgaccaca
      541 tatggggcaa aggctcggtg gcatccgatc caggtccagg gcagacatgg tgggtgtgga
      601 cgctgacgaa atagtggtga tgtaaatgaa gataagcaaa tacacttacc tcatggtcgg
      661 gctctctcta gtgataggtg cctacgccct gatcccgaat catgaatcgg gattctgcac
      721 tgacagctcc atcctgacga tgacgcttcc cgaaaaggag gggttcgata ccacgccgga
      781 attcgtcctt gaggcgaagc ttcccgacgt tcccgacacc ctcgggattt acgccataaa
      841 tggccccgac gtgacgaggg cttacgcctc ggaggtggcc agttccaggg gagccaaagg
      901 agaggtcctg gacgagggtt ccaacttctt aatcttgaat gagaggcaat acgtatctgt
      961 cgagaaggcc tccggcgcgg agacgctatt cctcgactac aagaacgcct ttgccgatct
     1021 ggagaagggg cttcctgatg caaatgtcct gaaacgaacc gccgacgcct atgccagtca
     1081 gaacaggctc ctccctgatg gatatcaata ttcggatatg tcctttttga cccggcaaca
     1141 gctgagcgag agagggccga cgggccgggc tgaaaacatg atggggattg caaagtactc
     1201 tcgaacccta gagggcctcc cggttgaggg tgctggctcg acgatcacag tactcatggg
     1261 cgatggcggc caggtccagg gctacaccaa agtatcgagg gttatcggcg ataggatggc
     1321 cgtagcctcc gccacccccg gaagagctga cagaacctac gagcttcttg tccctgatga
     1381 ggccttcaga cttctccagg agaaggggct gaccaccgag atcgccgacg ttgataaagc
     1441 cgtggtcact gacgtccatc tcgcttacta cgagacctca ggagatgaga ggcaggacgt
     1501 gaccgagccg atctacgtct tttcaggata tgccaccgga cccgatggag agtttgagtt
     1561 tcaggagttc atgtacgcat tgaagacgaa ggccggaacg acgccgttgc agaagcccga
     1621 caggagcagg gccgtcgaca ggagcaccat ccaggagccg ggcaagggga ctaaggacgc
     1681 tgagtcgagg acaatttcta cgatagcacc catcagagaa gtgtcgttga cgcctcgcct
     1741 gagcacgact cccctctcac aggtgaccta gaaactacaa acagaagaaa cggagacaag
     1801 tcgatattct cgggtcaaag gatcctggat aaaagtgtgg aggtcgacga gggactcgct
     1861 cttccggggt ccccaactct ttttggatga taaaggatat ctcgaaatgg agatgaaagc
     1921 gaagatgaaa gcgaagggga aactttcaag gatcgccatt ctcgggacgg tcctggtcct
     1981 cgtctcgacg gcgatctgca gtgggacaga agaggacttc tgcgatgacg gctacgccaa
     2041 actcaacgag acctcgacga ccgcggccat ctcgtacacg atccaccgcg cgaacggaac
     2101 gtctgcggtg gtcccgcctg gatctggccg gtacgaggcc ctcaacagca gcactatgac
     2161 cttcctcagc cagatcgcct acgcctccga gaggatagtg gagatcgagg acctggaggc
     2221 agagcttgaa ggcgaaaatt accttgaact cgcttccgac cagccgatcg tcatcgagac
     2281 ggcggaggag ctgaaccccg actggcctca gggctacacc atcgaaacga atcggatcat
     2341 aatcaagttg ggcgaggctg acgccgagga aaggatcttt accttccacg aggacgagtg
     2401 gccgcccata agaggatgga ggtctcttct gagggtcgag acgatcgatc tttgaaggat
     2461 cgcgaatatg gtcgcagttc acgagggggg tcaagagaag gggcgccttt ttcgagtaaa
     2521 ggttgaggag gatcgaaaaa aggatttcag gccccggcga cggcccgaac cttcgccagc
     2581 cccgcctcca gagcctccga gagccgttcc gccccgggac cgcctccctg ggcgatctcg
     2641 ggctttccgc cgcccccgcc gccgacgacc ttggtcgcgg ccttgacgat atctcccgcc
     2701 ttcaggccct tcgccagacc cgtcttcccc acggcggcga cgatcttcgc cccctccttc
     2761 tcgcttccca ggatggcgac gtagtccttc cccgagatca tggaggccgc cttgatcagc
     2821 tcctcgacgt cggcgttctc cagggtcgcc acgacgacct tgaggtcgca tgcttcgatg
     2881 gccctgctgg tcaggctctc catctgggcc ctcgccagct cctctttgag gcgggccgcc
     2941 tccttcttga ggtccttcca ctcctcgaag aacctggtgg cggtcttggg gagctgctcc
     3001 gcggggacgc tgagggcttc ggcggctccg aagaggaggt cgtctttctc ctgaccccgg
     3061 aggacggcgg cctcgccggc cgcaaactcg atcctctcca ccccgtcctg gacccgctcg
     3121 gttctgagga ccttgatggg gccgaccatc ccggtgttgg tgcagtgggt cccggcgcag
     3181 gcctcgacgt cggtccccat ctggacgacc cggatggact tccccggcgg gacgcccccc
     3241 tggtagagct cgaagccgaa cctcttctcc gcctcggacc ggtccagcca ccccgtcgat
     3301 atggggacgt tctccatcac catcctgttc gccaggagct cgatctcctt cagctcctcc
     3361 cgggttatcc tcttgtagtg ggagatgtcg aggcgggccc ggtcctcggc cttctgggct
     3421 ccggcctgcc agatgtggct tcccagaacc ttcttcgccg agtcgtggat gatgtgggcg
     3481 gcggtgtggt gtctggccag ggcgagcctc cgggagatat ccacctcccc ctcgatggag
     3541 tcgccgggcc ggacctcggc ggcggcggcc tccgactcct cctcggcgat cctgtggagg
     3601 acgacccttc ccgccgcctg gacgtcgacg acccgaaagg ccttcgaccc cttccggatc
     3661 aggccgcggt ctccgggctg gccgcccccc tcggggtaga agagggtctg gtccaggacg
     3721 accgccccgt cgacgaggtc gaggaccctc gcctggaact tcctctcgaa ggggcgctcg
     3781 tagaagagcc ggcgggtctc ggggaggtcg atcttcgact cctccttctc caacttctcg
     3841 gccttgatgt gggtgctcgc gacccgggag tagaagttgt cggggagctc gacctcgacc
     3901 ccctcctcca gggccgcctc cctggcgatc tccggcggta tcccgtggga gtcgtagagg
     3961 gagatcatct ccgagagggg gatcgtctcc cccctcttcc cgcgcccctc ggcgatcctc
     4021 ttcacgaggc gccggccctt ctccagggtc tcggcgtacc tctcctcctc ctggtcgagg
     4081 atctcgacga tggttccgag ccttccatcg aggtcggtgt agtcgagcct cgagatctgc
     4141 atcgatacca ggtcggatag aggcacctcg atggagaggt ccttcatctg ccggagggtc
     4201 ctccggatga cgaggcgggc gaggtagccc gccttgacgt tggaggggat gatcccgtcc
     4261 ccgagcatgt aggcgaggca cctggtgtgg tcagcgacgg cgtagacccg ctccaggggg
     4321 gtgatgatct cgtcgagctt ctccgccgag atcccgatct cctttgcgac ccggccccgg
     4381 aggtcccgga gggtcgcatc ctccaggtct accatcccgg cgaggcgagc gttcgaggcg
     4441 aggatccggg cccgctcctc gtcggataca gaagattgaa gccccgcgag atccgttatg
     4501 gtggccacca cgtcggggaa gatggcgtcg tatatcgtag gggagcccct ggaggcccag
     4561 acgaacctct ccaggccgta gccggtgtcg acgatgtagt tatccatccg gtcgtaccgc
     4621 tctcccttga tctcgtattc gccccggggc gagagcttca tgtccatgaa gacgagggtc
     4681 gcgagctcca ggcctcccat catcacctcg acggaggggc cggcgttccc
//
LOCUS       DASM01000030           12691 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_29167, whole genome shotgun sequence.
ACCESSION   DASM01000030 DASM01000000
VERSION     DASM01000030.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_29167"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2602 a         3878 c         3982 g         2229 t
ORIGIN      
        1 atgctgaaga tcgacaacaa gaccttctcc aagagccggg ggtacgtcgt ctgggtgatc
       61 gacgactacc tggagcaggg gctccacccc gacctcctcc ggtactacct cgcctcctac
      121 acctcccaca ccaaggagct gaacttctcc tggaagatct tcgccgagaa ggtgaacgga
      181 gagctggtgg gggccctagg gaacttcgtc aaccgggccg tcaccttcac caccaagaac
      241 ttcgaccggg ccgtccccgc cggggagctg gacccggagg tgatggcgaa gatcgagggg
      301 acggtatctc tggtgagggc tgccctcgag gagtacgagt tcaagaagct cgccgacggg
      361 gcgatggccc tggcggactt cgggaacgtc tacttccagg ggagcgagcc ctggaagctc
      421 gccaagtccg acagggaccg gtgcggggcg gtcctgagaa actgcctcca gatcgccaag
      481 gccctggcga tcctcctcca gccggtgatg ccggggaaga tggagctcgc ctggaggcag
      541 ctcggcctag agggcaccgc cgctgacgcc cgattcgacg acgccctcct cccgatcgag
      601 gcgggaatga ggctcggcgc ccccgagcac atcttcaaca ggatcgacga ctccctcgtc
      661 tcgaagctcg acgagatctt cagggagagg atagcggcga tagacgccaa aaaagggaag
      721 aagcagggag aagagaagat ggggaccgtg aagttcgaag atttcaagag cctcgacctc
      781 agggttgggg aggtgaaagc ggcagaaggg ataaaaggat cggagaagct cctccgcctc
      841 ctggtcgata taggctccga ggagaggcag atcgtggcgg ggatcgccaa gacccaccgc
      901 cccgaggacc ttttggggag gagggtcgtg gtcctcgcca acctagagcc cgcaaagctc
      961 ttcggcgtcg agtcccgagg gatgctccta gccgccgacg ccgacggcga ggccgtcctc
     1021 ctcgcccccg ataaggcgac ccctccgggg acgaagatcc gatgaccccg ccggatcagc
     1081 gggcggcccg gagcctcctg aaacggaggc atggggccgg tcggatcaga atggaagatc
     1141 gattatacat ttgaaagaat gaaatattga ggaggtcctt atattgctcc aggttgagaa
     1201 cctatccaaa gtctaccagc tgaaagaccg ccaggtggag gccctgaagg gggtgagctt
     1261 caccgccgaa gagggggaga ttctggggat cgtgggaaag agcggaggag gcaagagcac
     1321 cctcttgaag atcctccgcg gcatcgagaa cttcgactcc gggaagatcg agatcgacgg
     1381 cctcgagatc gtccccgact cggggcctga tctggtccac cgccagaggc aggtcaccgc
     1441 catccatctt cagagggact tcggcctctg gaccgagccc gccatcaaga acgtcgcacg
     1501 ccgggtctac tcccgcataa ccggctacga gacgatcccg acggagtacg acctcgaata
     1561 cgaggcggtg atggaggagg cgatggatta cctgaagctc gtcggcctcg ataagaaggc
     1621 gaagcacctc gccacgatcc tcagcggcgg cgagaagcag aggcttctga tcgcaaggca
     1681 gctcgccaaa aagccgaagc tcctcctcct ggacgagcct gccaccatgg cctgcccggc
     1741 gacgaagcag gaggtcctcg acgccatcaa gaacgtgaac cgggagcttg ggatcaccac
     1801 catcgtcgta tcccacctcc ccgaggtcca cagatacctg gccgatagaa tgatctggct
     1861 ggataagggg gagatcctgg acgaggggga accggaggag gtgctgagaa ggttcttatc
     1921 cctcctcggc gacgccgagc ccctagcccc ccgcccccag gccctgcccg ccatcaggat
     1981 tgtgggcctt cacaagagga actacctcgt agggaccggc gaggttctga ggatcgaggg
     2041 cctgaacttg gagatcagag agggggagat cacctccctc atcgggtcga gcggcggagg
     2101 gaagacgacc ctcctcaaga tcatccaggg gatcgcccgc ccggacgagg gctccgtctt
     2161 ctacaggctc ggagatggct gggtggacat ggtggagtac tcccgggaga ggatggcggt
     2221 ccggaggaac atggggataa tgtaccagga gttcgccctc ttcccgaggg agacggtcct
     2281 ggagcagatc gcctacaagc tcggggtgaa ggggcaggac gtcatcgact acgcccggaa
     2341 ggtggcggag gagatgggga tcagcgacaa gatcctggac gtcatctaca ccctcaccga
     2401 catgacggag gatgaggcta agacggtcct ggacaagctg ggtctgacga gggagatctt
     2461 cgaggagctc ttcccgcggt ttccggagac ggaggcgagg aggtacgcgg agccggtctt
     2521 caaggtcctg gacctcgacc cctctatcct ggagaagtat ccccatcagc tcagtggcgg
     2581 cgagaaggtg agggtctcca tcgccctcct gatggcctcg aagcccggga tcctcatcct
     2641 cgacgagccc ttcggggaca tcgatcccat caccctccgg ggggtggcca acgccatcaa
     2701 gaggatcaac gccgagttca acaccaccat cctcctcgtc agccatcacg tcgacttcgt
     2761 ccgggagata tcccaccggg cgatcctctt cgagaacgga gccgtcctcg ccgacggcga
     2821 cccggaggag gtctgcgacc tcttcgtctc ccgttgcgac gcctcctacc tgagggtcca
     2881 tcccgagacg aaggctccgg cggagaaggt ctgaaggggg agcggcatgg accaggagat
     2941 cggggaggtc tacgagcaga tcagggatat gatctccccc gaggagttcg aggagagggt
     3001 tggagaaaag gtcgagctga tgggggagct ctgcgaccgg agaacggcag cgatgctcgt
     3061 agcccgggag ttcggcgagg tggagctgaa gatcgaccgg atcaggcccg agacggggaa
     3121 ggtcaccttc gtcggaaagg tcgtcgccat ctctgaggtc cacgagttcc cccggagcgg
     3181 cgggggcgtg gggagggtcg ccaacgtcac cctcgccgac gagaccggga gcgtcaggat
     3241 cgtcctctgg gacgagctgg cggagcccgt atccaccggg gagctccacc tcgaccaggc
     3301 cctgcgggtc cggggcttca cccgggaggg gtacttcggg accgaggtga cggtggaccg
     3361 gggcggcctc gaggaggtgg aggcggagat caccacccgg acggagcccc tcaagatctc
     3421 cgaggtgagg gcggatatgg gggatatcca catcctcgcc agggtcctcg acccagggac
     3481 ggtccgggag ttctcccggc gggacggctc cgtcggcctc gtcaggtccg tcatcctcgg
     3541 cgacagctcc gggaagataa ggctcaccct ctggaacgag aaggcggaga tcgacctctc
     3601 cgagggcgac gccctggagg tgacgaacgc cctctcccgg gagaggtacg gctcagtgga
     3661 gatccaggcc ggcggctact ccgtcgtcag gaagagcgac gttttggtcg agtaccagga
     3721 gaagatgacg ccgatatcgg acctcgccgc cggatcgatc tgcagcatct ccggcttcgt
     3781 atccggcctc ggggaggtcc gggagttcga gcgggacgac gggaccgtcg ggcgggtggc
     3841 aaacatctac gtctccgacg gaacgggccg ggtgagggtc gccctctgga acgaccaggt
     3901 ccggctgatc gaggacctcg acctcggctc caggatagat atcgtcgact gcatggctcg
     3961 ggaggggtgg aacggcgagg tggagctgag ctgtgggtgg agcaccaggg ttactttcgc
     4021 cccgcccgag gagaggggtt aaggcctgag ggtggatgga ggattggtga aatatggctg
     4081 aagtattact cgaggatctc tccggggtcg gtccggccac ggcagagaag ctgagggagg
     4141 cggggtttgc cagcgtcgag gccctggccg tcgcctcgcc ggcgcagctg gcggcctgtg
     4201 ccgacgtggg ggagtcgacg gcggcgaaga tcatagcctc ggcgagggcg gccgccgaca
     4261 tcgggggctt tgaaaccggc gatttggtga tggagaggag gaagctcgtc ggcaaggtga
     4321 cgacggggag cgagaccttc gacgccctga tgggaggggg ttttgagacc caggcgatcg
     4381 tggaggtgta tggcgagttc ggctccggga agacccaggt ggcccaccag gtggcggtga
     4441 acgtccagct cccggcggag gagggcggcc tcgacggatc cgtcataatc atcgataccg
     4501 agaacacctt caggcccgag aggatcgccc agatggtgga ggggctcccc ccctccgggg
     4561 agccggagaa gccccgggag ccggaggaat ttttgaggaa gatccacgtc gctcgggcct
     4621 acaactccaa ccaccagatc ctcctcgccg agagcgccct agacctcgcc gagaagctca
     4681 aaaagtctgg aaagccggtg aggctcctca tcgtcgactc cctcaccgcc cacttccggg
     4741 ccgagtacgt cggccggggg accctggccg accggcagca gaagctgaac aagcacctcc
     4801 acaccctgat gcgctttgca gacctgaaca acgccgtagt cctggtgaca aaccaggtga
     4861 tggccaagcc cgacgccttc ttcggcgacc cgacgaagcc ggtgggtggc cacatcgtcg
     4921 gccacaccgc caccttccgg atctacctcc ggaagtccaa gggggagaag aggatcgcaa
     4981 ggctcatcga ctctccgaac ctccccgacg gcgaggcggt cttctcggtc acctctgcgg
     5041 ggctgatgga ttgatccggg ccttggccct cgacatcgac ggcaccatca ccgacgatcg
     5101 aaggcttctg caccccaggg cggtggaggc ggtcggaagg ctcaactccc gcaccacggt
     5161 catcctcgtc tcggggaacg tccactgctt caccagggct gcggccgtcc tcctggggac
     5221 gagcaggacc ttcatcgccg agaacggcgg cgtcatatcc tggggcgagg gagagatgga
     5281 gctcctctcc gaccccgcca tctgccagga ggcctaccgt cgcctaaaag aggtctaccc
     5341 gctggccaag aaggactcaa ggtacaggat caccgacctc gtcgtcgaat cgaacttcga
     5401 gatccaggag gccgaatccc gcctccgagg atgggggctg gaagccgatc tcgtcgacag
     5461 cggcttcgcc gtccacataa aggacaggag ggtggacaag gggcgcgccc tcgccaaggt
     5521 gatggaccgg ctcggcctct cccaggagga ggtggcggcc gtcggcgaca gcataagcga
     5581 ccttcccatg ttcagggtgg cggggtttcg ggcggccgta gccaacggag tcccggagct
     5641 gaaggcggag gcagactacg tctcagagga ggagttcggg ccggggttct gcgagatcgt
     5701 ggatcagatg gtcgatagag agatcctctg acccctcgat cgtctcaatt cagagccagc
     5761 ccagcccaca tgccaccccc agccccacca gcagtcctcc agcgacgacc ttcgctgccc
     5821 gctcccagtt gcggcgatac tctcgaccga gctgcatccc cacccaggtg aatatcaccg
     5881 agaaggatgc gatggttcct gccaccgcca gcggatgggc ccggcccagg ccgaggctga
     5941 aacccaccaa cagattgtct atgctcagtc ccgcagccag caacatcagc ccatgccatt
     6001 gagtcacccg ccgcgccaaa ttttcatcct tggaaagctc ctcgtcctcg ctttgatgac
     6061 ggaggccccc gatcgccgtc ggcagaccca accccatcag aagcaacgcg cccaccaggt
     6121 tcgtgtgaag gccgatgatc tccgcgatgg tcgagcccag ccatatgccg aggagaggca
     6181 cgaaaaattc gaagaggccg aagacgagca tcacccggaa gcggcgatgc gcctgcccca
     6241 gtgcccccag ggccagagcc actgcgaggt tgttggaacc gattacggca ccgatgataa
     6301 gaaaatcgat gggtgacatg gggactccct tgctcaatat tagaattttc ttccctcatc
     6361 catatccctc agaatccggg cccagttcgc ctcgcagacg atccaccatc ccagggtctc
     6421 tttgacgaac tccctcgctg cggggcagaa ggcgtcgacg gccaaatggt agggcatctc
     6481 cctgaggggg gggtggaggg ggtagcgagt gcaggtcttc ggccgggctg gatagatggt
     6541 gcaaccttcc tcgggttcgt agaaggggca gggctgccgg agggcccagg ccttcagctg
     6601 cctgtcataa ctgcagaggg cctcaagccg cttcatcggc cagccgaggt ggcggcagat
     6661 ccgctccgtg tcctcccgat cgaggtagag ccccgccccc ggggtcttgc agcagaggcc
     6721 gcacccggtg catcggaacc tctcccagaa gaggtcggca ccccggctgg tccccgccgg
     6781 atccctccgg tctttggcgg ggaggggtat ggcgatgtcg aagggctcgg tctccccccg
     6841 gtcgatggtc ttctcctgct cttcccgttt cagaaccgac cagtggggcc tcatctccag
     6901 gagcttcagg tggagccccg ctcgcttcat ctcgacgcag ccctcggcgc aggatgcgtg
     6961 gcagagcctc ttcgtctctg ccagcatctc ctggggcgag agccttcggg gggtcttttt
     7021 ggggatgatg atcgccgcct ccctcgaatg ggaccgtctc ttcggtccag gtttggaaac
     7081 gaggagtaaa aggttttatc ctcgacggca gaagttcggc aggggcgatg ggcgccagga
     7141 ccgtattcga cgtccctccg gcatacctgt cgcctaaggc ccgcgaggag atcctgcggg
     7201 ctatgctctg acttcggagg ctccaaagat aggagctcgc ccccaagaga cggataatca
     7261 cgacttccag gcgatcttat cccccttaac cttgatcacc ccttccttct tgagggtcct
     7321 ggccatctcc tcggccaccc ccgtcccgtc aaccttcatg acaaaaccct ccatatcctc
     7381 ttcgaaggcg aggagctctc tgaactcgtc gacgtcgatc ttattcgagt ctcccggcgg
     7441 cgcgagaagc ctctcgaccg tcatcgccat ggcgaagatt ttatagtact cctcgccgaa
     7501 ctcgtcggcg aactcgtcgg cgacgttctt cagcatgggc gttgtcatct cgtcgaggcg
     7561 gacctccact gtcttctcga aacggaccgc caagatcgac ctcgtcagct cgtcttcggg
     7621 gtcgcaatcg tcggggtcga tggttatcct tattaggggg tcttccccga gctcgcctcc
     7681 gggaggacca tctacctcgt tcagctccag ggcggcgtcg agaaactgga gagcctccag
     7741 cttctccaaa agctcagcag ccctatccat catctcctca tccgacgccc catcggccgg
     7801 ggcgacggac ccgtccttca agaaagaggc gatctcgtcc atcttaccct tccaagatgg
     7861 atccaggagg gaaaggtaac gatctgtaaa ggcctcagcc tcggccccct cagcgagcgc
     7921 ggccttgagg gccgacaggt accgatcgta ggcctcgaac tcctcgacac gccccctcag
     7981 ctggctagcc ctcccctcca cgtaggccgc gctctccacg aaggcggaga ttgaaggccg
     8041 aagatcgacc ctgagacccg cctctttgag gtgatccacg atctccgccc cttcggactc
     8101 ctcgtactct ccaagcttga acatgaggat acgagatgga ggagttttat ataatatctt
     8161 tggcgaaacc gaaaaaaaga aggaacggac ccaccccccg gcgggccgga gaacgatccg
     8221 atggtggcct agacgaaatg atcgggattg gtgggcgacg gggggggacg ccatctgccg
     8281 caacccccga tgcccagcca tcggccccac tcaatcgtct cagtcccttt gaatgcctcc
     8341 tcacttcgcc tcagggggct tcaacatgac gaaggcgacg actatgccca ggatcgccag
     8401 ggctgccact atcaggaaga agggctggaa ggcccccaga aggtccttcg cctgggcgga
     8461 gacgatccct ccaatgatgg cgcccgcgcc gtaggcggtg aagacgaggc cgtagttctt
     8521 ggcgttgtcc ttcatcccga agaagctcgc cgtggcagta ggagctatgg caagccatcc
     8581 gccgaggcaa ccccacagga tggcgaagct gacgacgtac gcgtagaagg aggagtaact
     8641 cgaatacatc agcaggcatg ccgccaggat gaggacataa gatagaatcg ccgcattcct
     8701 gggggtcagc ttgtccgtca gacttccgaa gatcggcctg ccagccccat tgaagatggc
     8761 gaagggcaag atcaggagga aggtccactt cgtcgcctcc tccgccgtca tcccggcgtt
     8821 ggagaagacc tccaggccca cggggcctgc gatccctatc gccgtgaggc ccgcaagggc
     8881 gccgatggtg tagcagagcc acagcccgta gaaggtcttc gtcttggaca tctcgttcct
     8941 catgaagtcg accttccgag tgacggtggt ggatggcggt gcggtccagc ctccgggctt
     9001 acagccagag ggggggcact tgaggaacat cgataagacc acagttatga tgatgaaagc
     9061 caccccaaag gtcctcatga tgttcattat gtcccagccg ctccccagca tgaacctgct
     9121 ggcgtatgtg atgatggccg acgagaagcc gaagcccaac accgtcaatc ctacggcgag
     9181 gcctcgcttg tcggggaacc actgggcaga ggtgtagatc ggcgcgccat aggcgattcc
     9241 tacaccgacg ccaccgatga cgccgtaaag gggtatcagc atcgtcgggg aggaggcgaa
     9301 ggaggccgcc agccagccga cgccgcagag cacccctccc gccatgcaga ccttggtggg
     9361 gcccatcttc tggatgtaag ggccggccag gggcatagtt aaggcaaaca tcgccaggaa
     9421 gacgatgaaa ggccaggtca tggccatggc cgtcggatcc tgccctaggg ccttgaagtg
     9481 gttcaaaagc ggggttctca ggacgccgta agcgtaaatg gcccccagac agagctggat
     9541 gatcaggccg actattacta tcatccaccg cccaccaggc ggctcgtagg tttcaatatt
     9601 ttctgccata atcagacacc ctccatggat aactgcctat gctcgctcaa agctcggacg
     9661 ctgccgaaag acctttccca aaatggcgtc tccctccaag agaacgtcat gccaaagatt
     9721 tgttgggctg aattattagg gtatcgtatc tatttaaaat tagcgccaat tatctcgaaa
     9781 atacgttatc cacatcagaa attcgccttt tatgattctt caggagcccc aggggaccga
     9841 tcaggagatg cgttctaagt ggaaataatc ataaaaatat tttgattatt gaaaatttat
     9901 attagtaact tccccgccta cctccccatc gggtcggtag ttggagaagg ccaccatcga
     9961 tgatgataat ggcttttatc ttagaaggct gtttagggga tggtgagttg gatgatcaga
    10021 gccaatccaa agaccagctg gaacgatctc gaatcgatgc ccaccatcgc caagaccgct
    10081 tacgtcgacc cggcggccac cgtcatcggt gacgtcagga tcggagagcg ggtctacgtc
    10141 gcgccgggcg cctccgtccg ggccgatgag gcgacgccga tcatcatcgg ggacgactgc
    10201 aacgtccagg acggcgccgt cttccacggc cttgagggga cgagcataag gctggggaag
    10261 agggtctcca tagcccacgg cgccgtgatc cacggccccc tggagatcgg ggacgaaagc
    10321 ttcgtcggct tcaatgccgt cgtccacgcc tccaccctgg gaaagaggtg cttcgtcggc
    10381 catctcgccc tggtggtggg ggtcaggctc cgggatggga gcttcgtacc gccgggcgcc
    10441 gtcgtcgacg cccaagagaa ggccgacggc ctcggcccgg tccccgaggg gctccggggc
    10501 ttcaacgacg aggtggtggg ggtgaacacc gagtttgctc aggtctacaa catgatggat
    10561 aagagctgca agacccctcc gatctgaagg tcccagatat gagggggcgg tgcagctccc
    10621 gcccccggca atcttttttc cgtcctcaat ccgagcccat caccgggttt atcttctggt
    10681 ggaggacctc ggccatctcc gcccggaggt tcaccatccc ttcttcgccg ccgtcgtact
    10741 ccaggaggtt tgcccggatc agcttctccg gaaactgggg gcaggcgccg cgccggcaga
    10801 ggacgataga ctcctttccg acggcgtggg cgatcccaag ctcgtacatc accagggggt
    10861 cggagccggt caggtccacc accaccatcc gggccctgca tatgtccctc cagatcgcct
    10921 tcagcttctc gtcgtcgtcg ccgaactcca gggccctccg ggggatgagc cccgcccgct
    10981 ccagggccgg cttaaccgcc tcgtcgaaga ggtcgtcgcc ctcgtcggtg agggggagga
    11041 ggaccgaggc ggtccgatcg tcgacgatgt aggaggcgtg gccgaagagg ggccggatct
    11101 gcatcaggct catcaggttt atcgccctca ggacctccgc ctcgacgtga gcgagggcga
    11161 tcacccggag gatgtccctc ctctccctct cgccctgatc gaggacgacc gccgggaaga
    11221 ctatcatcaa cggatcctcg tacccgaact cccaccgagc gctgctgatc gccttctgct
    11281 cagagatccg cttgtcgggg tggtacgtgt acatggtgga gagggcgccc gcatggttct
    11341 gggccaggat gtagagggtg gccgcgtcca gaaccgccac gatgctgctg gggttctggc
    11401 tccttacgac ccccctctga atcttcttct cctccgccag gccctccacg atccccatga
    11461 actgatccag gaggcggtca accttctcga tcaggtccgt aggcatatct ccgcctttga
    11521 tcctatctcc agaatgcatc atcacctata caccagggat gataagggac ggcagaatat
    11581 aaggcccttt ggtgccaggg gtgggaccga tcagaggatc ccctcggggc tctttctgaa
    11641 gccccggcgt ccccggggct tgactccaag aatttcggat ccagcgccag agaagggcta
    11701 tgtggaggag gggaacccat tagggccggg atatccccag gaagagctcc cctgccaacg
    11761 ccaccctgtc gtcgcgaaat tcgccccctc tagcgggcag ggcccaccca ggtgcagccc
    11821 cgaagccgtc gacgccaaaa aaagtgatga aaaagatgag gtggacgtgc aggttccatg
    11881 ccgtccgatt catctcgggt tcatttccgc ttaaccggcc ccaggggcag gacggttctg
    11941 ccgtagacct cgttgagcac ctgggcgcag gcggcgtata tggcggtgaa gccgcagaag
    12001 ataccctcgt acccggtgat ggtggctatg gtggcgctgc cggtgtagtc ccttatggcc
    12061 aggagccaga agaggatcgt cagggtgaag aagacgacct ggagggccct gttcagcttc
    12121 agggtgccta tgaacatgta gaaggtgaag agcccccaca tgaagaagta ggctatcagt
    12181 ccaggatccg aagtcttagt tcccgccgcc caggtggtgt tgggcaagag gatcagagct
    12241 acgagggtga gccagaagag cccataagag gtgaacgccg tcgtcccgaa ggtgttccct
    12301 ttcttccact ccatgattcc ggcgatgatc tgggcgaccc cgccgtagaa aatccccatg
    12361 gccaggatca tagacccgag ctcaaaccag cccgcattgt gcatgttcag gagcaccgtc
    12421 gtcatgccga agcccatcag accaagaggc gctgggttcg atgttccgtc tcttatgtcc
    12481 acgggaccga cgtgggttat gtcccggggc cttcctccac ccgtatctga tgctatatcc
    12541 tatccctccc ataaagccga aagattcgac gggaggccct gaaaccgcat cggatatatc
    12601 cttttccata tttaatgggg atctttacaa ttaaacatta taattgcggg acaaaatcag
    12661 attttttcac agctataaaa caatttttcc a
//
LOCUS       DASM01000031            5405 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_81340, whole genome shotgun sequence.
ACCESSION   DASM01000031 DASM01000000
VERSION     DASM01000031.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_81340"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1066 a         1597 c         1719 g         1023 t
ORIGIN      
        1 catgttctcc tacatcatga cctccaggag gaggaaggtc ctctccgacg acgaggatta
       61 cggctcctcg gcgagggagg ggctctggat cgggatcgcc ttcacccttg gcatggcgat
      121 cgtcttcttc gtcctcggcc tcttcatatc gaacctgggg gtcttcgtcc gcgactcgcg
      181 gttcttcgac ctcgccgccg gggtcctcct ggtggttctg gggatcaacg tcttcaagcc
      241 gatcggagag atcctggagc cgatcaaggc ccgcctcccg gcccggcggg aggaggaggt
      301 ggagttcagg gagaagaaag gtctgatgga gagggcggtg aacctctcca ttggcctctt
      361 caagcactcc gtcttcatcg gggccttcac cctcggggcc ttcttcgccc tcggctgggc
      421 gccttgcgcc atatccctgg tatttcccgc cctgatatgg ctgatctccc aggatatctc
      481 cgccctgacg ggagggctga tgctcttcgt cttcggggtg ggccatgggg tcccgataat
      541 ccccatcgcc accttcagca ggtccttcgg cgggaagctc ggggaggatt acctggccgt
      601 cggatctgcg atcgtcaagg ccttcgggat catgataatc gcgaccgggc tcgtctatgc
      661 ggcccgatac ttcggctaca agctctggta gcggccgaat agattataat gccggccgca
      721 aaatatatgg caggttttga tatgccggga aagccagaag agatcaagat ggtgagcaac
      781 gccatggcca acgtcccccg gaggaagatg atggccttcc tcgccggggg cgagaggacg
      841 tcggaagaga tcggggaggc ggtgggcaag tccatgctcg actaccatct caagatcctg
      901 gagggtgcgg cgctgatcga gatcggtgac cacaaggtcc gcctctccga gttcggcaag
      961 aacttcatgg agacaaaggc tgagaagccc aaggaggcgg tcgccgacct ctcgggggcg
     1021 aagcccgttg agatcacgga ggtgaggcag ctcctcccct gcatcgccga ctcgacgaag
     1081 tttcggatca tcgcccagat ggccccgccc ctgggcggcg ccctgaagcc cctggagccc
     1141 ttattcccga ggggtaggta ctccgagagg ataggggccc tgataatcca gcggggggac
     1201 ttcctgatca ccatctacgg gacgggaaac gtcaccatga cgatgataaa gggcgaggag
     1261 gaggcgagag gggtcctcgc cgagctgagg gagaagatca acgaggcgat agccaaaggc
     1321 gtggctccgg ccccccggga gaaggtgagg gtcgagccga tggagatcta ccgatacctc
     1381 ccccagacgg actgcggcga gtgcggcgag cagagctgct actccttcgc catcaggctg
     1441 atggctgggg aggtgtccct ggatctttgc aagcccctca aagatccgga gtacaggcag
     1501 aaccgagagc atctccaggt cctggtcgag tacatctgag ccggagggtg gcatgaagac
     1561 cctgacgatc atcctcgccg acggccccta catctccgag tacgcagaga tcgccaagaa
     1621 tattgccgat gccgccttga aacggtatca tgtgaacata ttcctctacc tcgatgccgt
     1681 ccacatcccc cgccgggggc agcgtcccgc cttcttcgca gacgagggag ggctcttcgc
     1741 cgacctcgcc agcgaggggg cggtcataag agcttgctcc cggtgcgccg ccgccagggg
     1801 ctatacggct ggcgaagacg gctccacacc ggattaccat accgggataa agatcaacag
     1861 cctctacgac ctcccggaga tgatggcagg aagcgagagg gtgatatctc tatgacggta
     1921 gggtcggcgt tctacctcct cgactctgcc ccttacggaa gcgagaaggc cttcggcctc
     1981 ctgaacgccg cggccgcaag cctcgggaag atggaggtca cggtggggct ctacggcgac
     2041 ggagtctacc tcgccctgaa aggccaggac ccgcgactcc tcgccgttcc aaacttagcc
     2101 gagatcctct acgcctacgg cgagatgagg gtcatcgctc acgagccctc ggtcctggag
     2161 agggggctct tttttgaggg gctgatcgag accgtggagc tcgccgacga ggaggcgttc
     2221 ctgaaggcgg tggaagagtc ggactgcctg atcctgcttt gaaggtgaaa gagtatatgg
     2281 ttttgagatc cgcagacgta tttctcctga caaagacgcc agaaagcgat aggtccaagc
     2341 tctgcatgaa gctattggca agatcggaga gggcgcggct atacctcgca ggggatggag
     2401 tctaccatct gctagaagat aaagcggtct ttccgccgtc ctgcgagata ttcgcctgcc
     2461 tagaggatgt tgccgcccgg ggcgtcgttg cgaggaagga ggtgaaggtt ctggaggagt
     2521 tctacgatac cctcgtcgag gacgtcatgg aggcgactgg caggttcttc tcgttttgaa
     2581 ctgcactgct acaagaatgt gattcacatg atcatcttga aaatagaacg ggacggttgt
     2641 atcagctgcg gccagtgctg gggaagctgc cccgagttct tcatcgaaga ccccgatgac
     2701 gggcgaagct cggtatcgga ggagcggagg atcgcgggaa agctggacga aggatcggcc
     2761 cctgatcaat ttgaggggtg cctcagggag gcggaagagg cctgccccgc cgaggtgatc
     2821 caggtgagcc cgatccagcc aagcttcgac tgacggggtg gtggatatgg tttcagatga
     2881 tgtttgtcca gaatatgaca agaaagggga gcaaaagacg gggaagaaga aggtcctctt
     2941 cctctgcact cacaactcgg cgaggtctca gatggcggag gggctcctgc gggacctcta
     3001 cggcgagagg tatgaggcca acagcgctgg cgtcgaggcg acggccgtcg acccccgggc
     3061 cgtctccgtc atggaggaga tcgggatcga catctcaggc cagcggtcca aggccgcctc
     3121 agagctggag gatatcgtat tcgacgtcgc cgtcaccgta tgcgacaggg cgagggaggc
     3181 ctgtcctcta tgcaccaccg acctcgacct tccatcgagg gcgccgaagg cgctggaggt
     3241 catccacaaa agcttcgagg atccggccgc cgtttccggg acggaagagg agcagctttg
     3301 cgccttccgg caggcgaggg acgagatcag agggtggatc gtcgagacct tcggatgagg
     3361 cgaggcggaa tatttgtgga agtattacct tgaaggtgat ggattgaaag atcaggatgt
     3421 caaaataacc gtgaaggaga ggtacggccg cgtggcgaga gaggcgggtt cgtgctgccc
     3481 ctcctcaagc tgctgcggaa gttcagagcc tgaagatatc ggccggagga tcggttactc
     3541 ggaggaggac ctcgcctctg tccccctggg atctaacctg gggctgggat gcggaaaccc
     3601 catcgccctc gcgtcgatcc gggaggggga gacggttctg gacctgggct ccggcgccgg
     3661 ctttgactcc ttcctcgcct caagcaaggt aggtccgagg gggaaggtca tcggcgtcga
     3721 catgaccgaa gagatggtgg agagggcaag ggccaacgcc gaagctggcg gcttcaagaa
     3781 cgtcgagttc aggctggggg agatcgagtg cctcccagtg gaagacgggt ccgtagacct
     3841 cgtcatatcc aactgcgtca taaacctcgt ccccgacaag gaggctgcct ttaaagaggc
     3901 gtatcgggta ctgcggcccg gcggaaggct cgccgtatcc gacatcgtcc tcctggcgga
     3961 gctgccggat atcgttagag attcggtcga ggcctacgtt ggatgcgtcg ctggcgcagc
     4021 caggaaggac gattatctcg cctcgatgag gtcggcgggg tttgaggagg tgagggtgat
     4081 cgacgaggtg cccttctccc tggattgcgt gacaagcgac ccggcgggcc gggcggccct
     4141 ggaaggtctg cccgacaaaa agcttatgga ggtcgaaagg gcgatcgtca gtataaaagt
     4201 cagcgccttc aagccaggct ctcccccagg acggattgaa agctgttaat ggccgatatt
     4261 aggcagagct gctgccagcg ggcttgcagg acccgaatgg gtggaggctg aggactagac
     4321 cctcaacccc tctttgatcc tctccaccac cctctcgcag tcggcggcct ccacctccag
     4381 cctcggactc ttctctatcc cgaggtcggt gaccaccacc gcgagggcgg cgggaaggcc
     4441 cgcttggtcc aggatcttct tcgcgcactg gacggggcag ccgtcgatcg ccaccagctt
     4501 tctctccttg gccggcaaga tcatggcgtc aatgtgggcg ccgatcccgg cgaggcagaa
     4561 gagcttccct ctcccttcct tcgccagctc caccgccgcc tggttggata tctggccgac
     4621 gttggagccg ccggcacatg ccagaaagag aaggtcgccg gaggcgcaga ggcacccctc
     4681 ctcctctcgt tcctcctctt tctcaagcac gagctctcac ctcttcagca tcccctttat
     4741 ctccgccacc gacggcgctc tccccacggc gacggcctcg ccgtcgatga agagggcggg
     4801 ggtcatcatc actccgcggt tcatgatctc ctgaaggctc tccaccttca cgacctccgc
     4861 ctcgaccccc gcctccgcca ccgccttctc gacgttctta gcgagggact tgcacttggc
     4921 acagcccgtt cccaatactt cgatcttcat catagcttcc tccatctcgc ctcggctccc
     4981 aagccttcat gggatatcgt acctgatcct atttatccgt cggtgaccac cagatccaag
     5041 acgctccgga cgtatccggc agagccggac cgagaaccta tgatgtccag gctatagatc
     5101 ccgggatcca catccgccac ccagaccccg gtgtatctgc cctcagctgt ctgctggagg
     5161 cttgtccttc ccgcctccgt cccgtcgcgg ctggtgaaga tggcggtgaa cttcaggacg
     5221 ggctgaaggt cttcggagga aggggaggcc gaagagccga tcctaacaac tcgggcccga
     5281 acctcgctga aagcggccgt tatcccaacg gatccgccgg ccttgacggg gtttggatct
     5341 gccaacacct ccaccaggcg gagctctagg ggaggctggt tctccagcca gtccctccag
     5401 gagta
//
LOCUS       DASM01000032            3958 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_56771, whole genome shotgun sequence.
ACCESSION   DASM01000032 DASM01000000
VERSION     DASM01000032.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_56771"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          677 a         1376 c         1124 g          777 t
ORIGIN      
        1 agcccgccgc ctgctcgaag gcccgagggc ccaggccggg gacctttttg agctcctccc
       61 tcgtccggaa ggggccgttc tcttcccgat agaggactat cgacctggca agcttcggcc
      121 ccagccccgc gacccgggac aggagctcta tgctcgccgt gttcaggtcc acccccacgg
      181 cgttgacgga aatttcgacg acctcgtcga gcttcctatt gagggccttc tcgtcgacgt
      241 cgtgctggta ctggccgnnc gatcttcacc agctccgcca gggggtcctg gagccttctc
      301 ccgatggaga cggcgcttct aatggttgcg tcctcctcgg gaaactcctc cctcgcggcc
      361 ctggaggtgg agtagacgga ggctcccgcc tcatccacca tgacgatggg gacgtccccc
      421 gaaatcccga gcccccgaag gaactcctcg gtctcccgtc cggcggtacc gttcccgacg
      481 gctatgaact ccacaccaaa cctctcgcac agatcgagga tcttcctctc cgcctcctgg
      541 accgcccttg ggggagggtg ggggaagatc acctccttgg cgaggaggga tccggaacgg
      601 tcgaggatga cgaccttgca tccggtccta aacccaggat cgatggccat gaccgtcctc
      661 tcccccaggg ggggctccag gaggaggtgg cggaggttct cggcgaagac cccgatcgcc
      721 ctctccatag ccctctccct cgccgccgag aggctctccc tctccatgga cggcgcaaga
      781 agcctcttgt agccgtcctc caatgtactc ctgacctcat cggtggcggg agaggcgggg
      841 cctctcctcc tccttcgcag aaagagcgac tccagaatcg ccgttgcctt ctcctgaggc
      901 gcctcgacgt ggagggtcag aaaaccctct tttttccccc gcaagatcgc catgacccga
      961 tgagacggcg ccttggatag gatctcagaa cagtcagcgt aatcccggta cttggacccc
     1021 tccccctcca gggggcggga gaccttcgac cgcagaatcc cctccctcag gaagagctcg
     1081 cggatcctgg acctagcccc cgggtcctcg ctgatcctct ccgcgatgat gtcgcgagcc
     1141 cccgccaggg cctcgtccac cgacccgacc tccctctcgg gatctacaaa cttcgccgcc
     1201 tcagccccag ggtcgatctc cccctggtcg aagatctgat ctgccaaggg cagaagaccc
     1261 ctctcccggg cgacggaggc ccgggtcctc cgcttcggcc tgaagggaag gtagaggtcc
     1321 tcgagggcgg cgaccgtcgg tgcctccaga atcctcttct cgagctcctc ggtgaggacc
     1381 tccctctctc ggaaagacct caggatggag gagcgcctcc ggtccagctc gcggatcagt
     1441 tctgcccgat cccgcaccgc cgctagggcg acctcgtcga gcccccccgt cgcctccttc
     1501 ctgtacctgg cgatgaaggg gatcgtcttt ccgtcctcca ggaggacgag ggcggcatgg
     1561 acctgcttct cccggaggcc gagctctctt ccgatgaggg cgatgggcag ctcattgatg
     1621 atagcccccg accggttgcc agagttcaaa gactaaaccc tccgcttgtt gggcctcccc
     1681 ttcttcacct cgaagaggta ggtcttctcc ccatcgacct cgtgccagag gttccggtag
     1741 taatcctcct tccactccgc gaagccgtct tcaaaccccg gctccccctc gtctatgtgg
     1801 atgaggtacc ccctatccga gatggctgag cagagtttca gcttctcaat gctctccttg
     1861 agccgggatc ggtggtaggg ggtagtcttc cccccttcct tcgaatccat cagaagctct
     1921 atcaccacca ggcactcttc ccccttgaag atcgagatat cggggcggca cccccccacg
     1981 gggcggttga tcaacatagt gattaattta tttttatctg tgtgggcccg gaggtggtga
     2041 taaagggagg ccaaaaagtc ttcctccgtc tcgatcttac cgctcctgcg gtcctgcacc
     2101 gatccctcga aggcgtcgac gacgccccgt tcgaacttac ttacgcgagc agacatctct
     2161 catccctcaa gccaccgggt tccagtcgtc tccaaactag ataaatccag agatcggccc
     2221 tggggtccgg ctccaatcgg gatcccaata gaaaacctac atatccccca tctccccact
     2281 aaagatagcg atgacgaact tcaaggtcct tctggagggc gcctggctgg tcagggacgt
     2341 caaatccgcc gacgacgcca taggggtcgc catatcggag gcgggaaaga ggctgaacag
     2401 caagaagctg gactacgtgg aggtggaggt gggccagacg gtctgccccg cctgcggcga
     2461 gtccctcgac ggcgtcttca tggtggcggg gaccgccctc gtcggcctcc tcttcgagat
     2521 ccgggtctac gacgccgagg gggaggagca cgccttccgg atagcaaagt cgacgatagg
     2581 aaaggccctg ggggctgtcc ccctcaggct cgtggaggcc gaggagatgg agggaggtaa
     2641 ggcggctaga tccaagaaga ccgatcgttc gatctaggcg ggtcaggatc gaacccgctc
     2701 ccactccgcc accttcgccc tgatgatcgc cctgctcgtc ctggcgtcgg tgaccaaaag
     2761 ctcatcctgg atccacttcg gaaggaagga gaataccccg ccccgcccct cctcccggaa
     2821 cctcctatcg cagaagagga tcaccccccg ttccgatgcg tccctgatca cccttcccgc
     2881 agcctgcaga ccccggttta tcgcaggaag ggtgtaggcg atcaggatcc cgttcttctg
     2941 gccgtacttc cttacgtagt actggttcac ttccctctgg atctcattgt agaagccgag
     3001 gggtaggccg acgacggcaa ctccgttgag agcctcgccc ttgtagtcga tcccctccgc
     3061 cagcttccct ccggagacgc cgaggaggac cgccccccgc ctcttccccg ccttgaagaa
     3121 gtcatcgagg atggcgggga gatcctcgga gttcctcggc tcggtgaaga ggagctttcc
     3181 caccccccgg gccgccgcct tgcagaaggg ctcgtactgg ctcatcatcg agtacnntcc
     3241 cgctaacctc ctctattacg gccctgacgt gttcctcaat ctcccgccgg ttcccggcct
     3301 cctccctctt ctccagctgg gtcgtcgcgg ccttggccac catcaggaga cggttctcct
     3361 gggggaaggg gttgggtagg ctgatcttct tagccctccc cctctccccc agaatgtaca
     3421 gctcgtacgc ctccagagga gatagggtgc cgctgagcat caccgtggcg tttatgttgt
     3481 cggtgatcct ccggaccaga ggggcggggt cgacgttgtt gacctcgagc caggccctcc
     3541 tcttctcgcc gttgccggcg acggtgatct tcctctggta ggagtcgtcc ttctccgcca
     3601 tctcgacgtc gttcaggaag aggaggatct gggcgaggct cggctcgatc tccccctgga
     3661 ggttctcccg gtccccctcc gccagctcca gctccgagac catcagggcc acctccgtgg
     3721 cggcggagag ggcctcctcg acgtcgtcga acccctgata gaggaagccc cggaagaggt
     3781 ctccgtccaa aagctcctcc ccctcctgca tcctggactg gcggctctgg atgtactgcc
     3841 ggagcctcgg gaggagggtc cggatcacct ccaccccccg gagcctcgat gaggcgtcca
     3901 gggacgactc cgggagcctc gcctgcccca tcgactcccg gtacttctcc acctcctt
//
LOCUS       DASM01000033           12408 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_37320, whole genome shotgun sequence.
ACCESSION   DASM01000033 DASM01000000
VERSION     DASM01000033.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12408)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12408)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12408
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_37320"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2679 a         3762 c         3547 g         2418 t
ORIGIN      
        1 ctggcaatag acccccctcc tggaaccatc gcctacatca aagactttaa gggccatgtc
       61 gagatactga atggaaatta tgacgcggcc tctgagatcc aatctctcgc cttccagaac
      121 gaggcgggtt gctaccggcg agaaaggttc tcggcaggga gctatgagat aagttacctc
      181 ttcaagatcc ggccgcctct ggaatatgac gaagaatacg ctcatcttaa cctgaagctg
      241 accgacgagc acatcccata caaagcggtt acaatagcca ttcacgaccc cgacggcctc
      301 gtccgtcgaa tattccctca tccacccatg gagacgaagc aagaggggga ggtctggcta
      361 ctgactggaa gcagcaaaga agatgaactc ttagaggtgg agatcctctt ttcgccggaa
      421 gtcctgaata tgatggaggg ctttccagga ttcgtcgccg atatcgagga taaaaccgta
      481 tctgcaaacg tgaaaagctc aggcaatttt ttcatgatgc tggaccggct attggcaatc
      541 ttcatcatca tactgcccgc gatcctttac ctggtctacc gccgatgggg cagggaagag
      601 ttcgtcgtca tccccgagac cctcagctat ctcccccaga agaggaagcc ctgggtggtg
      661 aacctggtct tcaacggaga tgtcttcgag gccgatacaa acggctttta tgctacgcta
      721 ctgaatcttt atcttagaaa tgtcatcgaa atagaaagaa ctcagggcac cgaagaggag
      781 gggcttaaga taagaatatc gaaagatcat aaggaagaat gtgacaaata tgagaagcaa
      841 gtgctggatt tcctcaaaag acactccaga gacggtgtct tcaacacgat cttattcgag
      901 atacatatcg atagattggt gagggaggcc aagacggggc gcaccaagga gctgaccaga
      961 gttcatgaag agatgaaacg tctgatgaac ctagtcgatg aggagacctc aaacgaattc
     1021 cttaataaat ctcccctgag ccatagaaga ttgataaact ctccaaaaag gtggattcga
     1081 tctctcagaa tttcagacct tttaaacgcc gcgccattta ttatattttt catatttttc
     1141 ataattttta taagagttcc cttgggcttt ctctggtcgc ccatcctctt caaggggttg
     1201 atcatcttta tgcagatgat cccggttttc atcgccccat cggccctctt tgggaggtgg
     1261 aagggaggat actaccggga gaagctggag tgggattcct ttaaaagtca cctatccgac
     1321 tttgctacca tccagaagta cgcccccgag gatctggaga tctggaaaga atggctcgtt
     1381 tatggcaccg ccctggggat cggggataag gtagccgagg cgatggaaaa gctcaacgta
     1441 atcatccccg aggttcacgt ggcaagcgag atgaactggt actttggtga tctctacccc
     1501 aggacatctc caccggtggt cacggcctct gatagcggat tcagcggtgg aggattcagc
     1561 gatggaggcg gattcggtgg agggggcgga tttggaggtg ggggcggtgg ggccaggtaa
     1621 ctcgacgggc cagccccgcc gtcgatccgc tacatacttg atggaagggc ttttcggtac
     1681 gggaccgaat ttgccgaaga aatctcggtc caactcctcc gtattgacgg ataggcggcg
     1741 tagaagggtt cgtgaagtca aaccgaggag gccctcgggg agaccctgga ggatgaagag
     1801 catcttaaga tggccgatcg tctccggtga aggcaagagg ccatagctcc tactttataa
     1861 ataaattgaa tcctaatcta aaaagagatc ccccgggaac cggggacggc atcgaccacc
     1921 tccaggctcc agggtgggga agccggcaac tccctccaag ctcaggcgtc caaccgcaaa
     1981 ctaaaatatc tatgatgctc atacatcaat tttacgagct tcgcgccctc tctgggcgaa
     2041 ggatggtcag atgaagatca agatcgagat cgaagataag ggcttgaggt ccactcacga
     2101 atttgagggg gccatccttc gagagaagat catcgatttt ttggacgccg ccggcgtctt
     2161 cctcgaggcc ccggatgatc tcccagcgga tgccctcccg agatctgccg atggccgtac
     2221 cctgcgcgac aggctggagg tcttcgtcag ggatgagttc ccaaactctt ggttctcgtc
     2281 taacgatatc aaagaccgat atgagatcat cgtcgacgac ataaagctca gcacggtatc
     2341 cacatacctt tcgaggatgt atcaagatgg ggtcctcgag cggcgcggca acagaaacca
     2401 gagacagtac cggctgatca ccgaagaggc cccctcaggg gtcgagtatc cgggaaccgc
     2461 attcaaggcc tctccttgga aggcggtgag ccggaggtag agccgtccag actccggaat
     2521 cggcggccgt tctcctggtc caacgtcggg ggcatctgaa cctgcagtcc ttccgccgag
     2581 agcctttcgc gtccataccc tcgcctcaat acgccctcta aaagggtcca agggctaccg
     2641 tgactcgatc aggcacccat caaggctcaa tccatccgac ctcaatcccc tcgtccagct
     2701 ctatgactgc cgcatatccc cgatgcatct ctccgacgtt gacgactcgg gtggggccga
     2761 tcgctccgta tccccggtcc tcgtggatgt ggccgcatat cagaatgtcg gggtggcgct
     2821 cttcgacgaa gcggcggagg ccaaaagacc cggtcctctc gccgctgtag atgaggtccc
     2881 cggatcctct cggcggctgg tgggtgagga cgatctttcg gcgacaatcg gatatatccc
     2941 catagagccg gttgagcgat cgatagatgt cgtcgtcggt gtggtagtat cgagccgggc
     3001 tctggagccg catgggatcg cggatcacca tgccaccaaa ccctatgaac ccgcagtctc
     3061 cgtgccgatg agagcggcga tggacgatcc tcagcccggc ggccttccac accgagggag
     3121 cagtctcctt ggggtccatg ttcccaggca cgatcaggac aggggggcca agcccagcca
     3181 gggcatcagc caccctcctc gcctcgtcgc cccgatccag gttgtggagg tctccgcaga
     3241 aggcgatcag atcggcttct atgccccgga gagcagatag ctcttcagaa cgcccgtgaa
     3301 gatcggccat ggcgagaatt ctcatctgac cagcaccaaa tttatatata atgtatttaa
     3361 tgactgagga taccgatctc ttattcagat caaatccttg tccacctcag aaacctcatc
     3421 acctcatcgg taagaacttg ccgaggatct tatcgctccc ccatgaggct catccagaac
     3481 ccaggggtcg aacgaatagt aggggtaaag gaagctcaag ctcgataatg cgacgggatg
     3541 atcgctctgg ttgagggtca actctgaccc caacaaggac cccagccacc ctatgccagc
     3601 ataatcgagt tcatcttctt ctcctcccat acccggctcc agggtgccat tgaccacatt
     3661 catccatcgg ggtgtcccac cccaggtcca gaggccgccc tcatcgcctc tggtgctgaa
     3721 tctctcctgg agagggatgg tcttgagcca tgaggctcca taatctcctc caacactcac
     3781 cgcctcgcct ccgcaggagg aagggctggt cgtcataaga accagaaggg ataaaacggc
     3841 gaccattctc atgaagaaac cccactatgc ctttgataag cactctctcc gtttcgataa
     3901 aagcctttct agatccaccg ccgaaggtct ggatttagat ctgagaagga agatcggggc
     3961 tgccctcctc ggctaccctg gtcggaaacc ggataagggc agagccaccc cccctggtgc
     4021 cgacgagaag gatggtgatg gcggaggggt caggggccag agcagccctc tcccctcgcc
     4081 accgcctcca tgatctcctc caggacctcg gagaggggct tcatatcggc atcctggaac
     4141 gggtaatgac accaccacca ttctccggat cgccacagat agagatggtc aggaccaaag
     4201 gccttgatca tctctctttt gaggctttcc catccctccg ttaacatcgc ctcagcgccc
     4261 ttatcgcccc tgtcccgaca ataggcgatg cactgaaccg gaaggtcaaa ccactccgac
     4321 ccccgggggg agggtctccg cccccgctct tcgaagggga tactatcgcc gatcaccgcc
     4381 cccagctcga agaggtcgcc gagaatcagc aacttccgaa ccatctcctc cgaatcataa
     4441 aaatcaaaga gtattgctcc aactcccgcc ggaaatccgt cactatggca atatatggat
     4501 aaaatactcc cgtcctcgtt ctgaacccct atcctgcatt cggtggacat gatggaccca
     4561 cgccttcaag ctcctccact ctcagacctg aacaatgacc gcatctaccc caactcctgc
     4621 ggcggacgcc atcctcttca agagactccg acaaagagct catcctctcg ccccttgaac
     4681 tggataatat ctccaccacc cgcaaaatat gtttcggtcc tgcccctcgg ctattaacgg
     4741 gggaggggtg agacccctta ccgcttccct gatcgagggg ttcgaagatc ggccgccagc
     4801 gatcgccgga tttggacgtg ggccgaatgg cgccatcgga tccgacgaac cagaagctat
     4861 aaatatctat aacgattaaa taacaccttt gccgcatttc gcgctgtaag aacgatgacg
     4921 agcataccgg caaagctctt caggcgagac ctgagaggtg ctgtctattg aggcagcatc
     4981 cggaaagcga tggagcggct gggaaccgaa aaggtcggat atatgcacgc cgtagcaggc
     5041 gtagtatatg ccaaaagccc agcctgtccc gagagtcctc gccgagggcg agatgcggca
     5101 caaagctctt gatcccctcc cattaactct ggaaaggaag gcaggatcta ttgccttctg
     5161 acccgactct tcctcggcct ccggagggaa ggggccgcca gagatctgcc ccccgtcgtt
     5221 gcctgtgctg gtccgcatcc gacccggctc cgccatcttc tggaaaccgt cctcatcctc
     5281 ggagtcgctc accctctggt ctcgtaaaac cctcatgacg ggatgcctcc ctccggcgtg
     5341 atctctccag gagaagattg gggggctgga aattaaataa taatacgata accgaaatta
     5401 ttattaatct atccatggtt tagagtggac tgatgtcacc aggtaagcca gcagacgcct
     5461 cggagctgag ggatgagtat gcccatttcg tcgggaggaa ggtcctcttc atcctcgctt
     5521 cggtggccgg gatcgtggtc ctggccgggg tcgccgccac cctcggctcc gccaagatca
     5581 ccccggtcga ggtctacacc gccatcctgg cccggttctt ccccgacacg ttccagacga
     5641 cgagcttcat cgagacgatc gtatggcagc tgcggcttca ccgggtgctg atggccatct
     5701 tcgccgggat ggggctcgcc gttgccggga ctgcgatgca ggggatctta aaaaaccccc
     5761 tggctagccc cttcaccctg gggatcgcct ccgccgccag cttcggggcg tccctcgcca
     5821 tcatcctggg ggccgggttt gccagcggcc agtggcttat tgtcggaaac gctttcatat
     5881 tcactatgct ggcatcgatg accgtctacg gcctcgccag gtacaagggg atcaccccgg
     5941 agacgatgat cctcgccggg atcgccatca tgtacctctt cagtgccatg acatcgttcc
     6001 tgcagtacat cggaaagtcc gagcaggtcc acgaggtggt attctggatg atgggaagcc
     6061 tcggccggtc atcctgggat aaggtggcga taattgccat ggtgactgtc gtctgcaccg
     6121 tcgccctcgt atacttcgcc tgggacatca acgccatggg ggctggggac gagacggcca
     6181 agagcctcgg ggtgaacgtc gagttcgtac gggtattatg catgatgctg gcatccctca
     6241 tcacggcgag cgtcatatgt ttcaccggga cgatcgggtt cataggactc gtcgcgcccc
     6301 acatcacgag gatggtcgtt gggggggacc atcggttcct tctaccggcc tcggccctcg
     6361 tcggcgccct catactcctg gccgccgaca ccgtcgccag gaccgtcctc gcgccggtga
     6421 tcctgccggt ggggatcatg acatccttcc tcggcgttcc gttcttcatt tatctgttct
     6481 tgagaaggag gaaggagtat tggtgaagat caccatcaaa gacctcagct tctcctacgg
     6541 cagcaggagg atcctggatg atctgaacct cgtcgtcgga gactccgagg tcctgagcct
     6601 cgtcgggccc aacggatcag ggaagacgac cctcatcaag tgcatagaca ggatcctgaa
     6661 gccaaaaggg acgattattc tggacggcgg cggcggccgc aacctccaga gcttgagccg
     6721 gcaggaggtg gcaaggttca tagggtacgt accccagtcg actagcagct ccctcaccac
     6781 caccgtattc gacaccatcc tcatgggaag gaggccaagg atggggtgga gggtcgccga
     6841 agaggatatc gaccgggtag tcgaggtgat gaggctcctc cacgtcgagg agttcgccct
     6901 caaggacttc tccgagctgt caggaggcca gaggcagcgg gtcctgatcg cccgggccct
     6961 ggcccaggag cccgaggtcc tccttctgga cgagccgaca tccaacctcg acgtcaagca
     7021 ccagctcgag gccatggata cggtcaggtc cattgtgaag aagaccggcg tatccgcggt
     7081 gatggccatc cacgacctaa acctcgcagc ccggtactcc gacagactcg taatgctgaa
     7141 ggaggggagg attaacgccg tcggggaccc catctccctc ctaaccaaag agaacatcag
     7201 aaccatcttc ggagtcgagg cggtggtgat gaaggacctc gatcggcctt acgtcgtacc
     7261 gatcaggtcc ctgaacggag gcggaccgtc ggattgaaag ccgggatccg aaatctgagc
     7321 ctttcgtctc gctgctctat cgcggttata aaatgggtct ttggagctgg cagggttctt
     7381 gttactaccc tgaaacgagt acaatctgta tcagttacat acactaaaaa aggagaatgt
     7441 aatttgagga aagcggatgc tatctaaaat cgtcgagatt tgcagggaaa aatgatcctg
     7501 ataattggat ttagggataa aaagtgctct ggaaatgagt aaatcgaaat cctctactct
     7561 tataaatata agctttttat tgatctggct tatatctcgt atcattttgg ccagtctcaa
     7621 tcgtccgtct gtgtagtccg ctaatgggac ttcagctgcc tttacgaccc ttcgttcctc
     7681 acagtccttc tcttcgattg ttcctaccac cccaagctac cttaagttcg cgcgccagtt
     7741 atatctccat ttctccggcc gctgccctcg ggaggagccc cgcccgccct gcgccacccg
     7801 gaggggctaa aggtgatata tatcttgaat gaacccctag catagtaaaa atccccctga
     7861 aggagggagc ccgttgaagg attatctgac gctagacgat ttccccatca agaaccagcg
     7921 gatcctggtg agggtcgacc tgaacgcccc catggacccg aacggaaaga tcctcgacga
     7981 caagaggttt cgaagccaca tcccgacctt gcgcgacctg gaggaggccc gggtcgtcct
     8041 gatggcccac cagagccggg cgggaaagaa ggacttcacc accctggaga cccacgccaa
     8101 aaggctcagc cagctcctcc gccgcgaggt cggctacgtc gacgacatct tcggctccca
     8161 cgcccgggag gcgatcaagg cgatgagggc aggggacgtc ctcctcctgg agaacacccg
     8221 cttctactcc gaggagaaca tcagccgatc cccggaggag cactcgaaga gccacatggt
     8281 ccggaagctg tcgccgctat tcgacctctt cgtcaacgac gccttcgccg tctcccatcg
     8341 gtctcatcta tccgtcgtcg ggttcacaga gtttctcccc agcgccgccg ggaggctgat
     8401 ggagaaggag atcgactccc tcgacaaggg ggtccgcggg aacgagcacc cctcgatctt
     8461 cgtcctcggc ggcaccaagg ccgacgactc catcaaggtg atccagaacg tcttccagag
     8521 ggggggggtc gatcaggtcc tggtcagcgg ggtcgtcgct accgtcttcc agatggctgc
     8581 gggggtcaac gtcggcaata aaaaccgcga gttcgtcgcg gggctcgact acgccgacca
     8641 ggtcgccatc gccaaagatc tcctggagaa gaaccccggc aagatcgtcg tacccactga
     8701 cgtcgccctg gagagggacg gcgagaggct ggacgtcgcc atggataagg ttccggagga
     8761 ccttctgatc gccgacattg gcctggagac gatcgtcgac ttctccaaga ggatccggga
     8821 agcgaagatc gtagtcctca acggcaccgc cgggatcttc gagaacgaga agttcgccct
     8881 ggggacgacg gagatcctga aggccgccac ggagtcggcc ttctccatag ccgggggcgg
     8941 gcacaccagc gccgccatcg aggagctggg gctcgagtcc aagttctccc acgtcagcac
     9001 cggcggcgga gcctccataa gctacctctc cggcgacctc ctccccggga tcgaggcgct
     9061 gaagaaggca gcccagcggt ggaggaagtg atccgggccc ctgccatata gggacgaaaa
     9121 cggggcggaa gatggccgcc cctgaagaac cggctccaag aagagctcaa ggggatggat
     9181 cgccaccccg gggggggcgc ctcccctcca gggcctccag gaccctcctc cttccctcgg
     9241 cgaggatctc cttcggcttc cccccaggcg cctccagggc ggcggccccc cgatggcctc
     9301 ccccgccacc cccggaggcc tcccccagat ccccgaggat cgccgagagg tcgaggccgg
     9361 cgaggacggc gctcctcctc gcccgcccgc tgatcctgca gaggtcgccg tggaccccgc
     9421 cgacgagggc gacgtccgcc ccgatctcga cgagggtcat cgcactggac ccctcgaagg
     9481 cccccacctc cgaggagacg acgatccagt cctccgtcca ctcgatctcc gcccgggagg
     9541 ctgcccgcaa ggcggcgatc ctcctggagg tgtccgtggg gacccgggag aggacctcca
     9601 gggcctcgga gtaatcgagt tctgccctct ccaggatatc ggccaccgtc cggaaggagg
     9661 aggcttgggc gtgcttgaac ctccccgtat cggtgatgat ccccaccagg agggccaggg
     9721 ccgcctcccg gctcggcccg attccgctcc tcgccagaat ctcccagacg atctcggcgg
     9781 tggagtcggc gggcctctgg aggtggaagg ctgccccctc caggaggtct ccctcccggt
     9841 gatggtctat gacggcgtaa tccttgaggg ggatgccgcc tacctgaagc cctacagcgg
     9901 tgtcgacgac caccaccagg tcgtactcct ccaccggcgg gtctatcagg atctcggcgc
     9961 ctatagcctc caggaggctc gccccggtcc tgctcacccc ctccaccgcc cccaggtccc
    10021 ccccgaaggc ctcctggagg gtgaaggctg cccccaaagc atcggggtcg gcgttcttgt
    10081 ggcagaggta gaggaccctc aacccccccc tgatcctcct cccgaactcc tcctcttgaa
    10141 tctccatcca gggaggctct cggcacgatg gtagaaaaag ctgtttccag catcgtcgag
    10201 aggggggacg gatgggcgcc gtcaaagccc ccgcatcccg ggcccgcgcc atccccactc
    10261 gaaagccttt tccagtcggg aagatcttat cggatctcat gaccgctccc ggcgcgaggc
    10321 gagggaccgg agggattcga tgcagatctt catcaccggg gctggcgatg tcgggtacca
    10381 cctggcccgc gagctatctc aggattacga cgtcacagtg atagatcaga agagcagagc
    10441 ctgcgagcgg ctggaggaga tcgacgtccg agtcctccag gggaacgccg ccaacgccca
    10501 gctcctggtg gaggcgggga tcgaggatgc ggacatcgtc gtcgccgtca ccggcaacga
    10561 cgaggtgaac atgatcacct gcatttcggc aagccacctg ggggtcgaga ggacgatagc
    10621 cagggtcagc aactccgatt acatcgacca gccgatgaag gaccgaaaga atatcggaat
    10681 cggcctcatg atctgtcctg agctggtgat ggcggagcgg atcgtaaaag agctctactt
    10741 tccttccatg gtcgagcgtc ggaggctcgc cgggggtaag gcggagctga tcgagcttgc
    10801 cgtcggcgag gaaatgcccc tggcaggacc gatctcaaag atcgatctcc ccgagaactg
    10861 caggatcgcc gccatcaaga gaggcgagga gatcctcatc gctcggaagg aggagtcgat
    10921 cagggcgggg gaccggctga tcctcacctg cgaggcgaag tcggtgccta agctgaggaa
    10981 gatcatccac gaggataccg cttctcacaa ggtgatgatc gtcggtggcg gcgtcgtcgg
    11041 attctacctc gccaagtggt tggagaagat ggacttcgac ctgaagctgg tggagatcga
    11101 caactatcgg tgccaggagc tggcggagga gctccccgac accctgatcc tcaacggcga
    11161 cgggaccgac atatccctca tcaaggccga gaacgtcggg tccatggacg ccgtcttctc
    11221 cgtcaccggc ttcgacgaga agaacctcct ctgctccctc ttggccaaac agctcggcgc
    11281 agataagatc gtcgcgaggg tggacaggtc cgactacatc gacctcttcg agatggtggg
    11341 ggtggaccac gccatcagcc ccgggagggt taccgtcgac gccgtcctga agctgatccg
    11401 ggggggggat gaggccatct cttcaggcaa ggaggaggca gagatcttgg agctccgggc
    11461 cgagaaggag gcgaagatcc tggggaagaa cgccgcggag aagatgccga aaggggcgat
    11521 cctcggaacg atcatgcggn nccaccctgg atacggaggt gaaggagggg gacaggatct
    11581 tcgtcctggc cctccccaag gcatccccga aggtgaaggc cctcttctcc gccgagcgtc
    11641 gcggatcttg aggagggaga tcccctaagg agctcctccc tctgccatcc ctcttcctct
    11701 ccgaaggtcg gctacgaagg cctcccggag ttttccattt ctgaggacgg cggcgggatg
    11761 gaaggtgacg aggaaccggc ccctgatgac ccggccccgg agctcaccga tcccctccac
    11821 gtccaggacg gccctcgctg ccgcattccc catcaggatg acggtcctgg gctggagcag
    11881 ctcgatctgg gcgtcgaggt agatccggca ggcctccacc tccttcctct tcggcctcct
    11941 gttctcgggg ggccggcact tgacgacgtt ggttatgaag acccgggacc tttcgatccc
    12001 cgcctcctcc aggcagcggt cgaggactga ccccgccctc ccgacgaagg gtctccctgt
    12061 cctgtcctcc tccctccccg gcccctcccc gacgaggagg atctcggccc tcgccggccc
    12121 ctcccccggg acggccgtcg tcctcccttc ggcgaggggg cagcggcggc acgatccgat
    12181 ctcttcggcg agggatcgga ggggatcttc gaccgccggg gatccgcctg acccagttct
    12241 ctcttcgatg gtcatgctga acgaaacgtt cttattcatt taagccagaa acgcccctcc
    12301 aaggtggtta gaatggtaga atggaccgga agcctcagaa agttcgacga cttgtattac
    12361 accatcgaga ggatcgtcta cggcaggggt cagctctccg ggttcacc
//
LOCUS       DASM01000034            6287 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_76358, whole genome shotgun sequence.
ACCESSION   DASM01000034 DASM01000000
VERSION     DASM01000034.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_76358"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1095 a         1995 c         2021 g         1174 t
ORIGIN      
        1 ccggccgcag gggcagggct cggagtcgat cttcgtcagg tccccgatcc ggtaccgtat
       61 gagggggatc gcccacttgg agagggtggt tatcaccagc tcccccgtct cgccgtcggg
      121 gagctcttcc ccggtcttgg ggtcgataac ctcgatgagg aacatgtcag accagatgtg
      181 gatcccgttc tgctcctggc actcggtgaa aagggggccg ctcatctcgc tcgtcccgaa
      241 gatgtcgtag gccttgatcc ccgtcgactc ctcgatcctc cttctggtct cgtcagacca
      301 cggctccgcc ccgaagaccc ccaccttcag gctggtgtcg tcccggatgg agacccccat
      361 cctctccgcc acctcggcga tgtggaggaa gtaggaaggg gtgcaggcga tggcggtgct
      421 ccccagatcc tgcatcagca tgatctgccg ctcggtgttc ccggtgccgg tgggcagaac
      481 cgtcgccccg atcttctccg ccccgtagtg gaggcccagg ccgccggtga agagaccgta
      541 tccgtaaccg acctggatga cgtcgccgcg gccaaggccg atggacgtca gggcccgggc
      601 gagggagtcc gaccacatct cgatgtcgtc tttggtgtag ccgacgaccg tcggctttcc
      661 ggtagtgccg ctggagacgt ggtaccgcac gatcctgttc ctcggaaggc tgaacatccc
      721 cgtcgggtag gtgttccgga gatcgacctt gtaggtgaag gggagcttca ccaggtcgga
      781 gagggccttt atatccgaag gggccacccc agcctcccgg aacctctctc gatagaaggg
      841 ggagtgatcg tagacgtaat ggaccatcga tttgagccgc tcctcctgaa gcctggagag
      901 gtcctcgagg ggcatgcgct ctatgtgcgg attccagtag tcaaacatgg ccatcaacct
      961 gaaaaccatc aatctcttgt acgataaata tcaatcgatc gcctccggcc acccaggcgg
     1021 ctcctacaaa ggcggctggc gatggaggag gtggggggat ggtccatccc gatcgccccc
     1081 aacggcaggc ccgctccggg ccgggccgcc cccggggcct caggaaatca gatatacgat
     1141 catcgccaag acctccccgg aggcaaaaat ggatctgagg gcaatagcat acctttccgc
     1201 ccttctcgcg gccctcctcc tggcgggacc gtcgtggggt cagctggcgg agttcgagaa
     1261 ggtcaaaccc gggaacgacg cgtcttcgcc ggtggtggtc atcggtgagt ccaacgaacc
     1321 ccagggcctc ttgccctcgg tgaaacgggt ccagttccgg accgagacgg gatggtccag
     1381 ctggatctcg gtgaaggggg tgaggctgac caccgccccc gtcgccatct cttgggagcc
     1441 gggcatcgta gatgtattca cgggggggaa cgacaacgac ctatggcata tccgcctcca
     1501 ggagcaagag agctggtccg actggtcgaa gatcttcggc ccacccccct acaacaagaa
     1561 cctcggcggg gtccacttcc gaaagtacga gctggcggcg gcgaccgggt cagaaggtgg
     1621 agactaccac ctcttcacct gggggcccac aaaccactgc ctctacaaga ggtgctgggg
     1681 tagcggaggc ggcgtcggct gcgacaggac cgccgactgg acccccctgg agggagagat
     1741 cggatcgagg ccctcggcgg taatcttcgg cgacgagatc cacctcttcg ccctggatcg
     1801 atcccgaggc ctcctctgga acgttgccac cctccccccc gtcggctcgg gggaggatcc
     1861 ggcctggaat gggtggcagc ccctggagtg gtccttctcc accccaccgg ctgcggccac
     1921 cgacggggag cagatccacc tcttcgccaa ggacccaggg ggcgggatag gccatctcgc
     1981 cctcgacccc gagacggggg aagggtcctt cgagacgata gaccaggcga tcgactcaac
     2041 cccagcggtg gtgatcgagg agggaaggac gcacctcttc gggatcctgg atggagagct
     2101 cgtccatctt gaaggcggcg gcagcggctg ggcggccggt gagaacctca agggctcgat
     2161 ctcgggccag ccgacggccg cctccgatgg gatgggggat atatgggtct tcgcccaggg
     2221 gcagggcgga tggctctggg ggatccgctt cgccccctag gacggtggcg gagaggaaaa
     2281 ggtagaaagt ggagaagagg gaaggtgggg gcgaaggagt tcccccctaa gccctcccag
     2341 ctccttcgag ggcctccctg acgagccggt tcgcctcccc tggatccgcc ctcccccggg
     2401 tcttcttcat caccacgccg acgatgaagt ttatcgcctt ctcgtttcct gccctgtagt
     2461 cggcgaccgc ctgggacgac tcttcgacgg ctgcccggac cgccgccacc acggcgtcgt
     2521 cctcaaccct ggaaagcccc ttcgccgcca cgatggcggc cggatccgcc tcggtggtct
     2581 ctcccggacc gtccaggatc gtccggatca cctcaacggc ccccttctcc gtgatcttcc
     2641 ccgcctcgat caaatcgagg ataacgacca tctgatcggg gctgaacctc tcgatggtga
     2701 cgtcccggta gttcagctcc cccttcagga cgtcggcgat ccaggccgcg gcgagcctgg
     2761 cgggcgccat ttctgccacc acctcataga agtttgcgac ccggatctcg gaggtgaggc
     2821 atttcgcgtg atcgtcggct atgccgtatt ccgcgacgaa cctcgccctc ttggcgtcgg
     2881 ggagctctgg aagctccgcc tccacccggg ggacccagtc ggcgatcctc atggagacga
     2941 ggtcgggctc ggggaagtag cggnntcgtc gtagtggcgg gtctcctgga cctgggcgac
     3001 ccccctcctc cgggcagacc tctgcctggt gatctcgtag gcgagggcct tctcgacccc
     3061 cttgtggctc gatatgttct tgacctcggc ccgttcgcct ccgacgaggg agatgttcga
     3121 gtcgatcctc atcgccccct cgaggttccc gtcgaagacg tccaagtact ccaaaatgtt
     3181 ccggagctta tcgaggaagg acctcgcctc cttgggggac cggaggtccg gctccgtcac
     3241 cacctccagg agggccatgc cgcaccggtt gtagtcgacc atcgagtact tcgcccggtc
     3301 gatcgtcccg gcgtggacga gccggccagg gtcctcctcc atgtggaccc tggtgatcct
     3361 gatcctcctc tcctcgccat cctccccctt gatgaggatc gagccgccta ggccgagggg
     3421 gaagtcgtac tgggtgatct ggaaggcctt ggggaggtcg gggtagtagt agttcttacg
     3481 gtagaatagg gtctccccct ggatcgcgca gttgagggcg agggcgaccc ggatggcgta
     3541 ctcgaccgcc ctcctgttga cgactggaag ggtaccagga aggccgaggc agacggggca
     3601 ggtgtgggtg ttcggcggcg agtcgtgata gtcggtggag cagccgcaga agagctttga
     3661 gttcaatttg ttcagctgga cgtggacctc caggccgatg atgacatcct tttcggccat
     3721 cctataccac ctccttcggc acagcctcgt gataaggagt cgcgccctcg taggccttgg
     3781 cggcccggag gacgacggcc tcgtcgaagt ggcggccgat gatctgaagg ccaatgggca
     3841 ggccgtcggc gaaaccgcaa gggacggaga tggcgggaac tccagccagg ttgatgggga
     3901 cggtgaagac gtcggccatg tatagcgata gcgggtcctc gatccgctcg ccgatcctga
     3961 aggcggggaa gggcatggtg ggagtcacca ggaggtcggc gccgcctgcc gagagggccc
     4021 tctcgaagtc ctgcttgatg agggtcctca ccttgagggc cttgaggtag taccggccgt
     4081 agtagccggc ggagagggcg taggtcccga ggaggatcct cctcttcacc tcggggccga
     4141 agcctgcggc tctgatattg gagaaggtgg tgtgccagtc ctggtctgcc ccgagccgga
     4201 ggccgtaacg gaggccgtcg aaccgggcca ggtttgaaga tgcctcgctc atggcgatga
     4261 tgtaataggc ggccagggcg tacctggtgt gggggagaga gaccttcttc cacgacgccc
     4321 ccagcccctc cagcttggag atggcgtccc agaccgcctt ctcgaccaat gggtcgacgc
     4381 cttcgccgaa gtactcctgg gggaccccca gagtcagccc atcgacccct ccctcgatcc
     4441 tcgcgaagta cccgccctcc gacctcgcag aggtcgagtc ccgggggtcg tggccggcta
     4501 tgacgtccaa aaccagggcc gcatcctcga cgctcagggt gagggggccg atctgctcca
     4561 ggctgttggc gtaagatacg aggccgtacc gagagacgag gccgtaggtg ggcttgaggc
     4621 cgacgatccc gcagaaggag gcgggacacc ggatcgatcc gccggtgtcg gagccgaggg
     4681 cgcacggcgc ctcccccgcg gccacggccg cagcggaacc gccggaggag cccccgggga
     4741 cccggtcggc gtcccagggg ttcttcgtcg ggccgaagca gctcgtctcg gtggaggtgc
     4801 ccatggcgaa ctcgtccatg ttcgtcttcc ctatgatgat cgccccctcc tccttcagcc
     4861 tctcaatgac ggtggcgtcg taggggggga tgtagccgga gaggatccgc gaggagcagt
     4921 tcgtctctat ccccttcgtc gagatggagc ttttgatggc tatggggacc ccggccagaa
     4981 gcccccccca cgggctccta tcatactccc gggccctctc cagggccgcc tcttcggata
     5041 agacgttgta ggcgttgagc ctgctcttat tgatcttcga aaagagggcc gatacctcct
     5101 cttcggccga agctcctgcc tcaagcctct ccttcagcat cctcagcata gcgatcacac
     5161 gatcctgggg ctcttgaagt agccgttctc cgtccgcgga gcgttcgaga gcgcctcctc
     5221 cgaggagagg gactctccca ccacgtcctc ccggaagacg ttggtgaggt cgacgacccg
     5281 gtaggtcggc tccacatcct cggtgtcgag ctcatcgagc tgatggaagt agtcgaggac
     5341 agagttgaag gaggcgacga agccctctct ctcaccctcg gataccttta tgctggcaag
     5401 ccagcttata tgatccacat cttctcctgt aatcatcgca atcccacgcc tctcgacgat
     5461 gatgaaatcc tgggaggcta tgaacctttt gccggtggcc tggaccctgg gcagacgcgc
     5521 cctgaaggga ctggggcccc caatggatgc aaaggcggcc cgaaagagaa gacgccagga
     5581 ccggatatgt caggagagaa aaacaaaaaa ggtcgtagcg gactcgccgg gatttgaacc
     5641 cgggactgag gctccggagg cctccgtgtt atccagacta tactacgagt ccagctagga
     5701 ttccagcact atctcgatgt tgacgttctt gggaacctgg atccgcatca gctggcggag
     5761 cgcccgctcg tcggcgtcca ggtcgatcat cctcttgtgc acccgcatct cccagtggtc
     5821 ccaggtggcg gtcccctctc cgtcggggct cttcctcgtg gggactacca tcctcttggt
     5881 gggcaggggt atgggacccg ccatgtggac gcccgtcctc tgggcgatcc ccctcacctg
     5941 gctgcagatc tcgtccaggg agcgggggtc ggtcccagat agccgaattc ttgccttctg
     6001 cataactcaa acaccagaaa aatttgagtt agcggggggt gatgttcatg cacatccccg
     6061 ctgcgatcgt ctggcccatg tccctgatgg cgaacctgcc cagctgcggg ctctccttga
     6121 ccttctcgat caccatcggc ttcgtcggca tcacggtgac gatggcggca tccccggcct
     6181 tgatgaaggc ggggttctgc tccttcaccg ccccggtcct ggggtcgagc ttggccttga
     6241 tctcggtgag ggtacaggcg atctgggcgg tgtggcagtg gaagacg
//
LOCUS       DASM01000035           19856 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_10393, whole genome shotgun sequence.
ACCESSION   DASM01000035 DASM01000000
VERSION     DASM01000035.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 19856)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19856)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19856
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_10393"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         3593 a         6143 c         6005 g         4111 t
ORIGIN      
        1 aaggtcctct gccccacgga cttctccgga ccctccgatg aggcgatcag cttcatcaag
       61 gggatgaagg gtatccgcga ggcggtcctc ttccacgtgg tgtccagcgg cgagagccag
      121 gcccacgtcg acgccaagac gaaggaggcg atggagaagc tgaacgccgt caaggcggag
      181 ttcgagaatg ccggggtcca ggcctccgta aaggtcgccg tcggcagccc cgccgaagag
      241 atcatcgcgg cggccgagga gggggacgtc tccctggtgg ccatgagttc tcacgggaag
      301 ggatgggtga agcagctcgt cgtcgggagc gtcgcctacg aggtggcgaa gaacggcaca
      361 aggcccgtcc tggtgctgag ggcaggaaag gcgtcctaag acttctagcc aaattttttt
      421 tcatctggcg ggtcgacatc cttcgtcggc ccgccggata caggacaaaa gcatttaata
      481 tggggagaat atccagtctc agattcccgt tccgagggag agatgaaatc acgggacttc
      541 ggcttaatat ccagtttagg aggtaaaaca tggagccacc gacgataata gatctaggtc
      601 tatttgccat cgttctcatg gtcttgttgg gacccttttt ggtcaaaaag atcgaacata
      661 atctggaagc gttcctcttc gttatggggg ttattgcagt aaccctatcc ggattcgaga
      721 gccacgataa cctcgtgaag ctttacgggg attccatccc ccacgtgggc tggaacatgc
      781 acctgatcat ggaggcgatc gaggagccgg tcatcaaggg catagtgccg gcagtgctgg
      841 tcgcgggcct cctattctac tacggaaggg acaaggtcca ggtagggatc aacgccctct
      901 tgaagaaggt tcccctcaag atcctggtcc tggtgatgat agtcatcctg ggcctcgcct
      961 cgagcatcat caccgccatc atcgcctcgc tcctcctggt ggagctgatc aacgccatgc
     1021 cgatggaccg gaagacccgg gtcaacatcg ccatcatcgc ctgcttctcc atcggcctgg
     1081 gagccgtcct gaccccggtg ggcgagcctc tatctacgat agccgtcacc aagctgcagg
     1141 gcgagcccta caacgctgga ttcttcttct tattcaacca gctcgcggtc tacatcatcc
     1201 ccggctgcat cgccatgggc ctcctcggga tggtcttcac cggcaaggcg gcggtggtca
     1261 agcagaaggt ggccgccgcc tcggaggaga ccctcaagga cgtcgtcgtc agggcgggga
     1321 aggtctacct cttcgtcatg gccctcctcc tcctgggaag cgggatgaag ctgatcatcg
     1381 acaagtacat cctgggcata ccccccgaga tcatttactg ggttaacatg atctctgcga
     1441 tcctggacaa cgccacgttg accgcggcgg agatcagccc catgatgggg aaggaacaga
     1501 tccaggcggt gctgatgggc ctgctcatct ctggcgggat gctcatcccc ggaaacatcc
     1561 ccaacatcat ctccgctcag aagctctcga tcacgagcaa ggagtgggcg aagctggggt
     1621 tacccctggg cctggtcctg atggcgatct acttcgtctg gatcttctac atccccttcc
     1681 acatcgagtt ccacttctga gatggcgagg gggctttccc ccccaccatt tttatttatc
     1741 aacggcctct caagaacccc ggttcatccc tggctttgga gttgaagatc cgcctcgaaa
     1801 gatgagcccc tcttctcttc tccatcgatc tcttctcttc ttgctcgatg ggtacccggc
     1861 ctcggggaga tccggcccat cagctctgat ccgaccgatc gattctcgcc ctatggctgg
     1921 ccctctcatc tctttttatc agttccgcat tccagcgcct ctttccggaa tatctattta
     1981 tagaatagag tttctgcggt ttgaatgggt tttaggctat gggccctcta aagaggagca
     2041 gaaaacagag ggtctggcgt atttaatata gagaaaatat ccggattgga gcctccggtt
     2101 caggggaggt gatcatggtg gtcctcatga tgggattgac ggcgatgaat ctgttgcgga
     2161 gggctctgag gatgaatgcc gtcggagctc cggcacgatc ttcacctgga ggtagaaaga
     2221 atggagatgc ctacactagt tgagcttggc ctatttgcca tagttctcgt ggtcctgctg
     2281 ggcccgtttt tggtccgaaa gatagaacac aacctggaag cgttcctctt catcatgggc
     2341 gtttcggcgg tcaccctctc cagcttcgag agtcacgaaa acctggtgag gttttacggg
     2401 gacgccgtcc cccacatagg gtggaacatg catcttgtga tggaggcgat cgaggagccg
     2461 gtattgaagg ggatcgttcc ggcggtcctg gtggcggggc tcctcttcca ctacggtcag
     2521 agccggatcc agagcatcat ctactcggtc cttaaggtgg tacccctcag gataatggta
     2581 ttcgtgatga tagtccttct gggcctctcg gcgagcatca tcaccgccat catcgccgcc
     2641 ctccttctgg tggagctgat caaggtgatg ccgatggaca ggaagaccca ggtgaacatc
     2701 gccatcatag cctgcttctc catagggatc ggggcggtgc tcacccccgt cggcgagcct
     2761 ctatcgacga tagccgtcac caagctccag ggggacccct acaatgcggg cttcttcttc
     2821 ctcttcaaca tcctggcgat atacgtgata cccggctgcc tcgccatggg gctcctcggg
     2881 atgttcttcg ccgggaaggc gacggagttg aaggagaagg cagcctctgc cgctgaaggg
     2941 tctctgaagg acgtcgtcgt cagggcgggg aaggtctacc tcttcgtcat ggccctcctc
     3001 ctcctgggaa gcgggatgaa gctcgtcatc gacaagtaca tcctctccat cccccccgag
     3061 atcatctact gggtgaatat gatctccgcc gtcctcgaca acgccaccct ggcagcggcc
     3121 gaggtcagcc ccctgatgga gcccttgacc ttgcaggcgg tgatcatggg cctcctcatc
     3181 tccggcggga tgctgatccc cgggaacatc cccaacatca tcgcagccca gaagctctcc
     3241 ataaccagca ccgagtgggc taagctgggg gtgcccctgg ggctcatcct gatggtcctg
     3301 tacttcgcct ggatcttcta tctccccttc cactacgagt tccacttctg agctcctggg
     3361 ataggggaga tcccacattt ttttaatacc taattcttca aaccgcctcg tcgaactccc
     3421 ttcggtagct ggctacgccg gaggcgccga cgatcgccgt cttgttgagg atcaggatca
     3481 tgaaggcctc gtcggggacc tccgaccact ggctgtggat cccaaacctg gacgccctct
     3541 caaagccgaa gcgggggtag tactccgggt agccgaggac gatgatgtag gggcagcccc
     3601 tctccctaag catctttatc ccgttgtgga tcagaagcga cccgatcccc tgcccctgcc
     3661 gctccgggag gaccgccata ggcgcaagcc ccatgcccgc caagggaggg gtcgcccctt
     3721 ctcccccgat ggaggcaggg ctgaagagga tgtgccccac cacctctcct ccttcctccg
     3781 ccaccaggga tagaaggtct ctgcagctct cccggagctt gtcgacgacc tcggcctcgg
     3841 ccggctggtt gaaggccgcc aggttgatcc ggcggatcgc ctcgacgtcc tcgggcctct
     3901 cttctcggat gatgatcatt taattcacct ggacggttgg ttattgagaa tcctctatcc
     3961 ttcagccgaa ctcggccacg ccgtcgcctc aaagctcaaa tgccggctcc gcggccacag
     4021 atcccagcag ctcctccgcc tccttcatct cctcctcgga cccgtcgagg gcgagccaga
     4081 cggacccctc ggccccggag acgccgccgg cggctgcaag cttcgccttc gctccggtga
     4141 gaagctcgat cgcctcgatc tcagagaaga cctcgccggg gaccggcaga agccgcggcc
     4201 cctcggcccc cggggcgttc agcttgtttg ccaaatcgtc gaggtcgcca aagatccgct
     4261 tctctaggcc gacgggaatg attagccgga cccggcggcc caccaccgcc tggatcgcaa
     4321 ggccgatggt gccgccccgg gggtggccga tgaggacggc agctctgttg gaccacggat
     4381 cgagggcgtt cgcccccttc aggatgacgt ccccctcctc caggtcgtcg acggcgttga
     4441 ggatcgtctt ccccttctgc cagaccccct ttttgatgac gacgtcgccg ggaaactcgg
     4501 acatgtcatg gagcatcccc gcgtcgtttg tcggcatcca cggagggagg gtgacgccgc
     4561 ggaagaacct ccgcctcgag aagccctcga cctggccgat cttcgccagg atctcctcgg
     4621 cgacgtagcc gttggtcgtc ccggcgatga tgacgacggt tcccgatttc agggccgcct
     4681 cgacggcggg gtgggtcgcg agggcctttg ctataaggcg ctttcctgct gctggggaga
     4741 ggacgaagga tctcatggga tcttcttatc atcctggaaa gaaaattgtt ttgctatggt
     4801 tggcgcaagg tcacctatcc acggcgtttc tgatgttgcc gatggccttc aggtcgatcc
     4861 ccctcggcgc caggtaccac ctgcccttat gctctctcat gtgctcaaga gcctcggggt
     4921 agccgtcgaa ggttaggcac tcaccgtcgt cgaggttgag gaggatgagg gcgcccccgc
     4981 cagcctctcc ccgatccagg gcggccctga gggcggcctc gatctcctcg cgatgcctgc
     5041 tcctcttccg cgaccccagg acctccatct ctcgggcgat ctcttcctcg tcgacctgat
     5101 atagatcctc ttcggacggc tcggcctcgg cgagctcatc tccgaccact ctcccgccct
     5161 cctctctccg ctccatatcg tccctccgac cccgtcagag cttcgcgggc tccccctccc
     5221 cggccgccca gcagaggtac tccagcgagg ggaactcgtg gatggtggtg gcgacgacct
     5281 tcccgcggcc taccttcccg aggacgagga cgggcctccc tttggcgtcc ttcagaatcg
     5341 tctcaaaccc ctcgccgggg atgaggtagc cgtcgcagtc ggcgtacttc gcggcgaata
     5401 ggcatgagga ggggtggtcg gcttccggga cgagctccct attttcatat tcgccgacgt
     5461 accggagggg aatgggaagc cagtcgtagt cgtgctcggg gaccaggggg ccgaagacgg
     5521 tgaggatgcc gcccttctcc agaaaatcgg cgatcctgga tctggcggcc cggagggagg
     5581 agagggccct ggagtactgg gggttagcaa agcccgtggg gatgacgaga accctgaacc
     5641 gggggaggaa ggggcttccc aggacctcgg gcttgacgaa ctggtgctcc ctccccgcct
     5701 ccgccaggag cttctcgaag aggaaggtct tctcgccgat gatcgcaata tctggcatgc
     5761 tagagggacc tatttcccgg gtcgtctaaa gctttacccc tcgcctccgg cctctccagg
     5821 tcccgccagc ccctctctcc tccgatcgcc ccttttttct atttattcag aattgcttcc
     5881 aatagattta ttactatata tgactataat ctcctcccag agggaataca attatggaac
     5941 aggaactcat gaggattggg gtctcgcttc cggaatcgct cctgaccagg ttcgacggga
     6001 tcatcgagca gaggggctac tcctcccggt ccgaggggat cagggacgcc ataagaaact
     6061 acatagtcca ctacgagtgg atgagcgacg tcaagggaga ccgggtcggg gtgatcacca
     6121 tggtctactc ccaccaccag cgggggctcg tcgacgccct gacgaacatc cagcacgagt
     6181 tcaccgacat catccagtcg accctccacg tccannaggc ggcggagaag atgatggccc
     6241 tcaagggcgt aaagcacgtg aagctgacga ccacctccct cggcgctgag ctgtgagccg
     6301 cccggcgatc gcggcccgga gggagatcgg ccgggatcta tccaccccgg tcaacgggag
     6361 aaccggacgc tatggatcct ccccggggga ggggcggata ggaggaaggg gggcttgcag
     6421 atgatggtca gggaggacct ctccggaatc ctcgtcctcg acctcttcgg cgaggcgcgg
     6481 atagcgaccc ggccgaaggt ccccgtcccc gtttacggcg agaggatcgt tgatggcttc
     6541 cggatatggg acccccaccg gagcaagctc gccgccctcc taaacaagag cctgcaggcg
     6601 ggggggctcg ccggcctcgt ccgagatctg aacctcttcc caggatcggc ggtcttatac
     6661 ctgggcgccg ccaccgggac gacggcaagc cacgtcagcg acctcgttcc cgcaggcctc
     6721 gtctacgccg tagagctatc gtcgagggcg atccgggacc tcctggggct ctgccagggc
     6781 cgcgataaca tcgtgccgat tctcgaggac gccttccacc ccgagaggta cgcccctctc
     6841 ctcgagcccg tagacctggt ataccaggac ttagcccaga ggaaccaggc ggagatcgca
     6901 aacctcaacg tcggccgcta cctgaagcct ggaggcgccc tcgtcctgat gatcanncga
     6961 ggtccgtcga cgtgacggcg gatccgaagg aggtggcgaa gggggaagtg gcgcggctgg
     7021 agggggtcga ggtcgtctcc gtcaccgacc tccgccccta ccatcaggat cactgggcgg
     7081 tggtggcgag gaagaggtag gaggtcccat agagggactt tccgcacccc ggggtttttc
     7141 agctgagaag ctctctcatc tcatttccga tgatgttcgg caggagcctc acgtatccga
     7201 cgaccgggat cctgaaccgc gcgaccccga tgacccagtc ctcgtggacc ggcatcaggt
     7261 agtttatccc gaagatgacg taggtatcgc cccccctcct cacgaagacg tagccgaact
     7321 catgatcgag gtaggtgccg ttccctacct cctctatcaa ccccttcgcc atctggtcgt
     7381 tgaagtagct ctccttgacg acgccgagga gctggcctgc gttctggtcg atctcggagt
     7441 tgttgtcccc cttggtgatg tagccggcga agggggcgga ggggcccccc tcccacatcg
     7501 gctccccctc gtcgacccat cgcatcgccc ggtggatgac gggggtggcc ttgtcggggg
     7561 ggtaggggcg ccggaggagg acgtgagccg cctggtcggc cagggtcagc ttatccttgc
     7621 cgtagggccg gtagaggatg acgtttccgg gtttgttgaa ggtggtgtag ttctcggcct
     7681 ctccctcatc ccaggggacg acctcggtcc tggaggcccc ctggatcact atgatgtccc
     7741 cgaccttcat gttggggtac atgctccccg actccaccgc caccatgggg gtccagaccc
     7801 ccagggtcac ctgagataat atcgatatgc cgcccaccac cgcggcgacg aatatcagat
     7861 cccggatgaa gccctggacc tgcccctctc ccttgagcca ctctagaagt ttcatcggag
     7921 tgctgtatcg gcgccctaac gatataaccg ttttcggtcg tacctccttt tggtgcgaag
     7981 ggacccttta tctaagggct ccgaagatat ggacctctga tgcgggagat cgttcagcgg
     8041 ttcgccgagg aggggtacat gatagatccg gaggccctgg agctgatagc ctcctacccc
     8101 ggccccagag acgagctggt ggagggggtg atccgggggc tcgacccctc caccctggtg
     8161 gtgggagccc ccgacgtcca gcggtgcccc cgatggaccg atggcgtcgg gggagcgggg
     8221 tccctcccca agaccccggc cccttcgagg ggcgctggat ctctcccgaa ggcatcgccg
     8281 cccttctcca gagaagttcc cccctccccc ccgcctctga ggggggtagg ggatctgctt
     8341 cctgaagcct tcgcgacggc cggagccgtc ggtgctccgc cgtccccccc ttcggagctc
     8401 atcaccccta aaaaggcatc tcatccggca tcgcccccgg tggtggagct ctccccccct
     8461 cccctcgggg gcggggagcg ttctctggat ctacccgccc cggagacggg tggcggctcc
     8521 tttgggctga aggatgcccc cgatccgggg ccggaaggag ggacgggggt gttgaggaga
     8581 tcgagcccga tcccctctgg aaccgggggc tcggaagtca tcggtccggt cctcggcccc
     8641 agcccgggct ccgcagctcc tgaggagctc cgccttcccg gccctccagc ccccctggag
     8701 gccctcaggg acatcaccgg gaggtccacc tgcgtcgggg agtaccagga cttcgtccgg
     8761 tacttccggg accggtactc tcggatccgg gagatcctcg cggggaggct gaacgcaagg
     8821 ccgatcgaga gcctcaggcg gggggacctg gcggggaggg agatctccat catagggatg
     8881 gtgatggagg tgaggactag cgccaagggg aacagggtgg tggagctgga ggatccgacg
     8941 gggatggtga cggcgatatt ccagaaggcg gtccccggct ccgaggaggt ggagaccctc
     9001 gtctccgacg aggtggtggg ggtcaccggg acgacggacg ggaaggggag gttcttcgcc
     9061 aaggccctcc tccgccccga cctccaggcc cgatccgccg ctgctttcgg ggaggggggc
     9121 gccctcctcc tctcggacgt ccacgtcggg tccacctact tcatggagga ggcgtgggat
     9181 cggttcatcg gctggatccg cggggaggtg gaagaccccc tggggatagc atcgaaggtg
     9241 aagtacgccg tcatcgccgg ggacctcgtc gacgggatcg ggatctaccc cggccaggag
     9301 ggggacctgg ccataaagga catctgggag cagtacgaga gggcggcgga gctggtggcc
     9361 gagatccccg accggatctc gatcgtccta tctccaggaa atcacgacgt ggtgaggcag
     9421 gcggagcccc agcccgccct ccccgagagg ctgcggcccc tctttggggg gaacgtcacc
     9481 ttcgtcggaa acccggcgtc cttcatcctc gggggcgtcg actggctcgt ataccacggc
     9541 cggtcgatag acgacctggt cctgaagatc ccggggctat cctacgccga gccggagaag
     9601 gcgatggtgg agatgctgaa gagaaggcac ctctccccca tatacggcaa ccgggtctcc
     9661 atagcccccg aggaggagga cctcctggtc ataagaaggc cgccggcggt cctccactcc
     9721 ggccacgtcc acaccgtcgg gatggcccgc tacaaggggg tgacggccat caacagcggc
     9781 acctggcagt cccagaccga cttccagaag aagatgaaca tccagcccac ccccgccatc
     9841 gtcccctacc tcgacctatc gacgatgcgg gcgaggaggc tgatattcgc acgaaccccc
     9901 cgggatgatc ggtcatcgga gggacctctg ccggcgtagc cttcttctga gaatgccgtc
     9961 ttggccggcg gcatccgtcg atagagcccg catccgatcc cttcgggcct gggttttccc
    10021 ctctcctttg gggtacgatc ctgaggcgga agctctcgat tagagcagac tggaaatgaa
    10081 atgcagatat ttttatatat catggcggtc caagtatgcc atgatcggcc gcgttggggg
    10141 aatttcggca tgagggacat attcaaggac gctctgaagg aggtggtcca gtcgatcctt
    10201 cccatcagcc tcgtcgtcct cctgatccag ctcttcctcg tcggctcacc cccggagaag
    10261 atagccacct tcctcctgag cgcattcatg gtggggctcg gattcacctt ctttctgatg
    10321 ggggtgaagg tcgggatgct tccgatgggg gaggcgatcg ggggggagct cgcaaaacac
    10381 gggtcggtcc tcttcctcgt cctcgtgacc ttcgtcatat cctttctggc caccttggcc
    10441 gagccggacg tccgggtcct gacgagcatg ataacctccg tatccggcga ggtgatctcc
    10501 agggttgccc tcctcgtctc catcgccttc ggcgtcggga tcttcgtcgt cctctcgatg
    10561 ctccggatcg tctacggcgt cccgataaag tacctctacg ccggaagcta cctattggtc
    10621 atcgccctct cctttctcac ccctcccgat tatctcgcca tatccttcga ctccgggggg
    10681 gtgaccaccg gccccatgac cgtccccctg atcctcgccc tcggcacagg cgtagcctcg
    10741 gtgatggcgg ggaggtcggc cctgtcggac ggcttcggcc tcatcggctt cgcctccata
    10801 ggtcccatcc tgggcgtgat gctcctgggg gtgctcctgg tttgatcgcc gaggcggtgg
    10861 aggctctgac ggagtcgtct ctggacgtcc tcaaggcgat aattcctctc gtcctattct
    10921 ttctggtctt ccaggtcttc catctccgtt atccgaggag gcatgtgatc aggctcctct
    10981 tcgggatcgc tctcaccacc gtcgggatgg tcctcttcct cgcgggggtc ttcctgggct
    11041 tcctccccac cggccaggag atgggccgat tcttcggagg ctatgaagag gggtgggtgc
    11101 tcctggccgt cggagtcctt ctgggatttc tcgccaccta tgcggagccg gcggtccggg
    11161 tcctctgcta ccaggtggag aaggcgtcgt cggggtacat caagtccggt ctgatgctct
    11221 ataccctatc cctgggggtg gcagtcctgg tggggatcgg gatggctagg atccattacg
    11281 ggatacccat tctccacata gtaatcccag gctacatcct cgccatcatc cttttattcg
    11341 cgagcgacag ggacttcgta ggtatatcct tcgatgcggg gggcgtggcg acggggccta
    11401 tggccgtcac gttccttatg tccgtagccg tcggagcggc gtcggtgatg gaggggagcg
    11461 atcccctggt ggacggcttc ggcctgatcg ccctcatcgc cctcgccccc ataatcttcg
    11521 tattgtcttt gggcgtaata attcggatga ggaagggtga tgaaggatga agtgcgagac
    11581 tgatctctcc ctcatcgtca ccatcgtcaa gaagggatgg ggcgatgaga tcctctgcgc
    11641 ctccatggat gctggcgccg agggggggac gatcctcttc gggaggggcg tcggagttcg
    11701 cgagcagaag aagctcttga acatcatgat cgagccggag aaggaggtgg ttctgaccat
    11761 agtctccacc tgtgaggcgg accgggtcct cgaggcggtg gtgaaggcgg gaaagctcga
    11821 ggagccgggg aagggaatag cgtttgagct taggatcgat aagctccttg gcagggccca
    11881 taaagctgga gttgggggca tcgccgaagg cgataaaggg gagtccgggg agaaggatcc
    11941 gcccccggag gatggttaga gaaggcctag gagccgctcc catctcccgc cgagaggttc
    12001 ttcgcctcca tgaacttcac catccggtag agggtggcgt tggtgggggt ggggactccg
    12061 acctcttcgc cgaggcggac gacggccccg ctgagggcct cgatctccgt cgattttttc
    12121 gcccggatgt cctggagggt cgaggattcg tgcctcgccg tcggcggcag cagcctctcg
    12181 tagaagacgg ccctgtactc atcgggggcc gaccagtggg tccggtaccc cgccgcctcc
    12241 atcactgcga agatctctcc gatgagggcc tccatgatct ccctggaggc ggagacctcg
    12301 gccaggtcgc cgtaggggac gccgaagatc gcccccaggg ggttcagggc gcagttgtag
    12361 agcatcttcg cccagaggtc ctttcccacc tccgggacga cctcggtggg gatcccgccc
    12421 tttgtgatca cctccgcgag aggcgccatc ggcgcgaggt ccgagctgaa gaggctcccg
    12481 aggtggatcg agtcggcgtg gacggtcacc tcgacgacgt tcttctcggg ccttgaaaag
    12541 ccggtgatca cccgggcgct gtagatctga tcctttggga aatgctccgc gaagatctcg
    12601 gcgttccccc agccgttctg gaagaggacg atcttgccac cgtcgccgag gatcttccgg
    12661 tggcgggaga ggtctgaggc ggcggccggg gagccgtggg acttggtgca gatgaggacg
    12721 tagtcgtagc caaagtcgcg gtcccggttg agcccgccat cctggagccg ttgccctccg
    12781 atccggagaa gctcgtccag ggaggcgaag gccccgaacc cctccggccc gacctcgacg
    12841 gagccgaaga tcccggtcct ggccaggcct cccgtcctca gggcggagac cgtcccctct
    12901 cgcgcgatta tatccacctt tgcaccggac aaaatcaggc agcttgcgat ccccagcccg
    12961 acggcgccgc ccccgtagat cagagccctc atcctttcgc cttcctgggg gctttgacgc
    13021 ctcaacctac ttcacccctt cccccgccag gccctccagc cagcccgcca ggcaggaggc
    13081 gagaggagga accgggccag gggcgggagg gtttttatgg ggacgaccct cagccttccc
    13141 tcaaatgacc ccgcgagcct ctcctcggcg aaggacgggg cgacctctgc cctcaggggg
    13201 gcgacggcct ctaggggcat cagaaccgcc atcatcaggc cgaaggcctc cgcggtatcg
    13261 gcggggtcga agaggccgac ggagaggtcg attcggaggg actggacctg aactgcccca
    13321 gagaggcccc tcagcagatc cccgaggctt ctgagaaatc cccgggttcg gaggagttcg
    13381 agggcgatcc taggggagct tcgacccctt ccctcccctt catcctctct ttctccttcc
    13441 tctctttctc cttcctcttc tttgccctcc tcctcctcct cttctgccgg tggggcttcc
    13501 tctccccctg ccatcgaagg ctcccgcccg ccgccgcgga agaggtctct ctggaggcgg
    13561 ccgaagagcc agctcaccct caccctcgcc tcggggcggt caccgcctct tgcgaccctc
    13621 ccctccaggt cgacggggac gaggaggaga agggccagca gaacgaaaag aaggagcagg
    13681 agctggagga tcagggccgg ggggcccagg atggcggggt gggtcatggt ggggcgtccc
    13741 cctcctcctc tctactcgac cttgatatcc tttcccttcc ccttctcccc tccgctcttc
    13801 tcctccgcct ccttctcctt ctccttcccc ttcctctcct ccctctcatc gatctccttc
    13861 tttatcagcg aggggatctt atccgccagc tgctcgatga gggagcccgg cttcttctcg
    13921 acgggctcga cccggaccga ctcctcctcg atgatgataa cggcgacggg cttgatgccg
    13981 aagccgccac cggcgccgcc gctggtcccc tcgtcctcct tccccgcccc cgccccggct
    14041 ccgaagccgg cgccgacgct caagaggggt attacagtct ttcctccggc ggtgatcggc
    14101 tcgccgatga cggtcttggt ggcgaggagc ctctccatct ctccgatggt ggccttcaag
    14161 agagcttcaa cttcggtcat aatttatcct ctaataattc tcttctccta ccgttaaaaa
    14221 ctttctctgg ggatggcgcg gcagcgtacc gaacttcttc gcctcttagg gatgaagact
    14281 gccaccatca aaggaaatat ttcggtgaaa aagattattc tcctgtgcct tcttaccgtc
    14341 tcctctctga tatctttgga gtccgcctct gatctccttt ttgagcttaa tagcgcaaag
    14401 gtcatatatt cccgacgccc ctaaaaatat ggcgatgact gaatcgatga agatcagtta
    14461 ccgcaaaagt tgaagggggt aaatatgaga ataaatgggt tagtttccct ggtgacgata
    14521 ttaggcatga ttttaattgc cttttcgtcg gtctgcggag cggatgaaga gggcgggatc
    14581 gccaccgaag gcggctttga gatcctggcc gagtcctccg tcagccttga gatggaccag
    14641 ctcagaggct ccgacgatcg ggttcagatg gcagatcagc atcttggagc agaggggttc
    14701 tcgccggata aagagatgac ctcggataat ttcttcggca tgaggcagag ctataacaga
    14761 accggggagg gggatgaggc ggatcagagc tacgagttct tcattcagga gtactcaaac
    14821 cccggctcgg atatggccgc tgccgtcggt cggatggcgg tgaagagctc cgacggggca
    14881 tatgcggccc agtacacctt catcctcaaa gcccccaagg agaacgtctc cgcgatcgag
    14941 gagtactacg tcgcagcctc tccttcgggg ctctcggtgg tggaggcgaa gagctggtgg
    15001 acgtgtatgc tgggccagct ccctctggtc ggggaggagt gcggtctggg gcccaacgtc
    15061 tgcgccccgg cggcgacctc catcgtaggc tatctggggt gtgtggcgac ccactgtggc
    15121 cccagcttct cgaagtcgtc ggcctgctgc aactgcggct gccgccagtg gtgcagctgg
    15181 gcgtatggct gctgccggat gtgatccccg ggaggggccg gcagccaaat cctttatttt
    15241 tcccatcaat tcgggcacca aaggcgccga tatcaggcac tttattatat gggcgttacc
    15301 atcttattaa tcgatcttta taggaggtaa tctatcttga gtccggatgg ttggagagtt
    15361 ggaccattac ttgcagcgat ggcgctcctg gggatgggcg ccttgggtct cgtggcggcg
    15421 gcagggtcag atcaggcccc gatcggagtc ggtggggcgg aggagggacc attgaagatc
    15481 ggggcgatat acaacctgga ggggtcccag gcgcccctgg accttccctc ctcccggggg
    15541 gcgaggctcg ccgtcgagga gatcaacgcc gcgggaggga tcgacggccg gagggtcgat
    15601 cttctgctat gtgacggaaa gagcgatccc gcagaggttc gccgatgctc cctcgatctg
    15661 gcggaggaga acctctccgg gatgatcggc ctctcggata cggatatggt ggtggccgca
    15721 gcccccgggg cttccggcgc ccggattccc ttcctcacct cgggggccac ctcccctcga
    15781 ctccctgagg tgtacgatgg gcttttcctc gcctgcttcg gcgacgacgt ccaggcggcg
    15841 gccggggcag agcacgccgc tgagatggat ttgttgacct gcgcccttct ggtggagggg
    15901 gagatggagt acgcccgcct cctcgccggc tacttcaggg agcggtacca ggccctcggg
    15961 ggcgagatcg cctttgaggc cttcctcaac ggctcggata aagaggccct cctccaggcg
    16021 atcggggagg caaatccgga tatgatatac ctggcggcgg ggccggatct ggcggcggag
    16081 gtcgtcgggg aggtccggga tgcggggatc gagatccccg tattcggagg cgacagcctc
    16141 gactcccccg agctgaggag gggggggacg ggaaggatcg tcttcagcgc ccacgccctc
    16201 ctggacgaga gctcgtcgag gtgcgggccc ttcgtcagag cctacgatgg cctgtacggc
    16261 cacccccccg agaacgcctt cgcagccctg gggtacgacg ccgtaatgct cctggcggag
    16321 gcgatggatc gagccggctc gggggatccg gaggcgattc ttgaggccct ccagaacaca
    16381 acgggatttg ttggggttac gggggatata agctacgatg gtggtcgccg catccccaat
    16441 aagggggtga cgatgatatc cctccaggac ggggtgatcg atggctcggc ggtggtggtc
    16501 ccgggccctt cggtctgatc ggcataatcc gggacgtccc ggagaggtca ggtcccgagg
    16561 ctccgggccg gactccgcct cacggggaga gttggggcta aagagggtcc agcgccctct
    16621 ccaggtgctc ctggaggacg gcggcgttgt tccgctcctc gtcccgggcg ctgtagacca
    16681 gcgtcagatc cccggatcgg gccgcctcca ggaggggctt ccaggcggct ctatccgtct
    16741 ccagctcctc ccggtatcgc ctccgaaact cttcccatcg ggaggggtcg tggccgtacc
    16801 accgccgcag cccgtcggag ggggcggcct ccttggtcca gccgtcgacc ttgagatcct
    16861 cctttccgat ccccctcggc cagaggcggt cgacgaggaa gcgcctcccg tccccggggg
    16921 cgggaggatc gtaggcgcgc tttatccgga tcatatatgt caaaaataat ttctttttat
    16981 gttattaata ccttcgtcag cctcatctcc gatgtcttcg cggattcccc gcccaggagc
    17041 cgtcccccct accgttctcc ctactcccct cccaacccac cctcatcctc cgagccctga
    17101 ccttcacccc tctcctccgc ttcttcctcc acaccttcct cctccgtccc gcccgaggat
    17161 ctccgagcct ccatgaagag ctggtagact cgcgggtagt cggtcttagg aaatcggagg
    17221 gagacggtcg tccttctagc cgccggcggc ggggagccgg gatccgacgc catgagcccc
    17281 tctccatcga tggggggccg atcggcgtag atgttcgtcg tataccgcca aaatccccgc
    17341 gccggcgaca tttgcgtcat acggtcgcgg agccctccgc ggccttcgtc ccgtagagat
    17401 agcctaaaga tcaccatctt gaggaagcgg ctcaggtcgg aggcggtctc aaactcgatc
    17461 cagatcactc ccggctcctc ctcttcgcag gagttggcgg tctcgatccc ctcactccag
    17521 agggcagcga ggaggggcgc taatccggcg tcgacggcga tctgccgccc cgaggggtgg
    17581 agtaccctgg tctggcggtg gtctggcttc atatcatcag ggcttcgtat catcaggggc
    17641 gtggcgatca ctcaaagggt cggaaggagg cggtcggcgg ggccgccctg gaaaggggag
    17701 gatcatcggg gctctggttt gtcgggctgg ccccggatcc tctccagcgc ctcggcggcg
    17761 gcggcctgga cggagggggc ctcatcgtgg agggcctcgg caaggggccc cgccgccctg
    17821 gagtcaccga tccggcagag ggcgatggcg gcgttccgcc ggacgtgctc gtaggggtct
    17881 ttgagggctt cgatgagggg gccgacggcc tgaagatcac cgatcttgcc cagggaccag
    17941 gccgccctcg accggacgct ccagaccggg tcccccaggg cttcgatgag gggttcggcg
    18001 cccctcgggt ctccgatcct cccgagggcc tccaccgccc tccaccggac ccccctcctc
    18061 tcgtcctcca gggctcggat cagaggatcg gtagccccgg gatctcccag gaggcctagg
    18121 gcgaaggctg ctctcgaact gaccatcatc tctccatcct tcagggcttc gatgaggggc
    18181 tctgtggccc tggggtctcc gatcttacct agagaccagg cggcccgctc ccgaaccttc
    18241 ccgtccccgt ccttcagagc ttcgatgagg ggtccgacgg ccctggggtc gccgatcctc
    18301 cccagggcgt aagccgccat ctgacgggca tgcagatcgc cgcccttcac agcccgcaac
    18361 aggccatcaa tctctgccat ctcaggccac ccggaagatg atcaagataa ttgtcatcga
    18421 tctcaactta tgcatccttc ccttcatacg ttggtcgtcc cccgccaaag atgcttcgat
    18481 agcagagcag aggtccacat tcatagtctc gatggccggc gtcaggatcg acccctaccc
    18541 ccgggacgct cccagaatag atcctagaaa aaaatctgga ggactaaaaa tggtggggtt
    18601 aagaggttta tttataaatt tttataaata ttatgctctg gtcgagacca ttgcctcggc
    18661 ctttgcggct ttttatccgg ctccccctgc atcgagcctt ccattccccg gatagcatct
    18721 aatccgtggt cttgatctgt tggccactat catctcttga cgaccacaac cttcgacgct
    18781 ctcggttctc catctgtttt tcccttcatt ttacccttct cagacccttt tcttgcgtca
    18841 ttttcgacaa ggtctaaatc aggtggggtt tttcttgcca tatccaaata ttttataccg
    18901 catctatata accatatctc atctccatga tagctccgga gattctccgc cgcggggtat
    18961 tgttctaccc agatagtttt gtcccatcaa acgcaaaatc atagaaccga agatggggga
    19021 gggagtactg aggggaatct taagatcttc gtcttctaca ttccaactat ttatataaca
    19081 atgtcattta tattaaaaat ttataactcg tgtttcaatt gaaataaata ttatgtattt
    19141 attgtaattt aataatgata tcgataatgt caaaaatgac acaaataatc tgctttaaaa
    19201 aggattaaat agtaatttat cctatgcatt ctttggagga atttctcatg ccagaagagg
    19261 gattagactt gaaagaagga cgttcagtcg agggctcata taagggaaca attgtgctct
    19321 tcaaagacgt cgattgcaga ggggatgcca tattattgga gaactcatat ggcgtaaaaa
    19381 atctgaaaga tcttaaatgg aacgatagga tgtcatcgtt cttaattgtc agtggattct
    19441 ggaggttcta cgagaatacg gactggaaaa aagtgatggg acaagactgc caccccggaa
    19501 cataccgcga ttgcaccaaa gttgggatga agaaagggaa gttgtcatct cttgaatgca
    19561 taggtccgta ataaagaaaa ctccgtccga tgtaacttcc cgggcggctt acgccctgtc
    19621 gtctcgccca tctgcattag acttaaaacg aggcgttgag gccggatacg tccggggagt
    19681 tttcggcatg ggtttgggga ttcgatcaat ccttggcgca atacgcctct ttgcgcatct
    19741 tctatttctt atcctcaatg gcccgggtgc ctgggctaga agatacaacc ttaaaaaatg
    19801 ctttaagaaa agttagtgcc cagacccgga ttcgaaccgg ggacattcag atcttc
//
LOCUS       DASM01000036            5298 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_62074, whole genome shotgun sequence.
ACCESSION   DASM01000036 DASM01000000
VERSION     DASM01000036.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_62074"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          952 a         1621 c         1482 g         1241 t
ORIGIN      
        1 atcagccaga ggtcgtcccc accgaagttg tcaagggtgt agcctgctat gatgtagcca
       61 ccgtcggctg tctgctggac cgaatttcct ccatcctggc ccgatccacc gaaggttctg
      121 tcccaaaccc ggtttccagc cgagtcggtc ttaatcagcc aaacatcttt accgcctaag
      181 tcgtcttggg tataaccggt tatgatgtag ccaccgtcgg cggtctgctg gactgaattt
      241 ccccaatcgg ccccctccca tccgaaggat gtgtcccatg ctctgtatcc ggccgagttg
      301 gtcttgatca gccataggtc gtctcctccg aaggcgtcga gagttctgcc tgctatgatg
      361 tatcctccgt cggccgtctg ctcaactgaa tatcccccat cctggcctgc tcctccgaag
      421 gtccggctcc atccttcgat gcctttctta tccgattcgg ccagtacccc ccgctccggt
      481 cggtggtctt cggcggctga cgactctttc tcaccgacgg tcaccttttt ggagttagag
      541 cttttagccc cgtcgtcgtc agttacggtc agaacaacgg agtatactcc ccctttgata
      601 tatttgtagt caacacaggc gaacccctgt ttgcgccccc cttctccgaa atcccataaa
      661 tagtcgacta tcctgccgtc caggtcttcg ctctcgtctg catcaaaggt cacgatgccg
      721 tccgcccggg ggctggtggg ggcgtaggag aaggctgcta ccgggggctt atttactctt
      781 agatccctgg tggtgctgtt tactgcgtcg ttgtcatcaa ttatttccag ttttataata
      841 aagttgcccc ctttcagata attgtggatg gcttgtggcc ctctcgcggt cttcccatcg
      901 ccaaagttcc accggtattc ggttatcttg ccgtcggggt caaggctttg ggatgcgtcg
      961 acggcgatcc cttcaccaac gttcggctca ctattattga cggaaaactt cgctataggg
     1021 ggcagattta tctttatggg aattgacttg atccccaggg cccggttgct gtctttgacg
     1081 gtcaggctaa cgttgaaggt gccactcctt gggtaaaaat tggttgccaa accatcggat
     1141 agctccacgg tcgtatggcc gtctccgaag ttccacgtaa atgaggccac ttccccgtcc
     1201 tcctcgtcgt agctctctct ggcgtcgaag gttatgagat atcccgattt ttcatcctcg
     1261 atcgaatagc tgaactcggc cacggggggc tcgttcacct cgatcgagga tatggtccgg
     1321 ttctccttcc cctccacgtc ggttactttg agcatgacgg ggtaagggcc gcctttctgg
     1381 taggtatgat tgacaacagg gccgttttct ctaattccat ccccgaagtt ccactcgtag
     1441 tctgcgatct ctccatagag gtcgtagctt tgggatgcgt cgaagattat cggttctctg
     1501 gcgttgggtt cttcgacctc ccatgagata accggattgg gtggtatgtt gggtgggttc
     1561 agtgagacta tgggaagatg ggggtttacc cggacgaaaa ttttatgatt atcaactgga
     1621 taatagggcc cccccgggtg gtcagcccac cagtccaggt tgagccagac aaagcccgac
     1681 tcgtccacat gtgcatatat tttttcagaa ttatatttct ttttcaacca cgtggatgtt
     1741 ttattaactt tattatcatt gctattatgt ttgccaatac agacctcggt atatgcatgc
     1801 ccttgccatt catcataaac cagattgatc ttggcagttc cgccaatatt ttctattaac
     1861 gccgccatta ggatggcgaa atcgtcacaa tctcccgccc ctgcccggcc gatctcctct
     1921 ccaagtcgga tggtttggtt tgcataacgg tagaattctc cgcaccgtgg atctccggta
     1981 taattccagt tatttttaag gtattcgaag atttcgcaga cctgttccac cgaatgatcg
     2041 ccaggaaatc gttgcgccaa cgaagcagcc ttatcgacga ctttttggtt gcccacttcc
     2101 actcttttct cgatctcctt ctttatggag gctagatctc tctctccggg acagatggtt
     2161 ttttcgcccg tatcgcctga tctgctggtg acggtaaagg tagctatctc tgactcgttc
     2221 agttccgata gggaatactg atccagcttc atcgagccgg attccccgag ggcgtaggct
     2281 ggctcttcac aggatgttat atagcagcat acaatcgata ccgacagcaa cagactcaga
     2341 atcttgcgca taatatctct ataatatcaa actttttaac atatatactt ttctttgagg
     2401 gctcgtggct gtccattttg gggggcttca ttctgaccat cgatcttttg ttttgagccg
     2461 ctttaagtct cccacctaat cgggataatg ggctttgatg atgaaattgg cggatgattc
     2521 cgatgtcgtg ttaatataaa cctcgttggc ttctgatgga cggaatggcg cgtcatctct
     2581 gattacgcag gttttaggga acctcaatct tctgcgccgc ccgccggacc atcggcccgt
     2641 ccttggagcc accgtgaaaa tcgttggcgg cctcttcctc acctgcagac gaatatgtgc
     2701 ctcgtcaggc tccggtgtac cctctcctga tgagcctcca aaatctcgaa ccccgcctcc
     2761 tcgatcgccc cgccgacggg ccggtcttcg acgaatacca tcctactgcc ggggaccagg
     2821 accctccgca gctccgcaag gctcccagcc agaagctcgt ctcgcgatcc ggcctggatc
     2881 agggccgacc tcccgtaggg ggcgtcggag acggcgacct cgatggattc gtctttgagg
     2941 gggagcctca tggcgtcgcc gacgatgagg gtgcagtcga gcccctccag gttggaggcg
     3001 gcgcccctga caatatcttc ctggacgtcg acccccaggc cccggacccc caacagcccc
     3061 gcctccacca gaaacccggc ggtgccggcg aaggggtcca ggagccgctc gccttccttc
     3121 acttgagaga ggttgaccag agctcttgcg agcttgggga ggatcgcccc tgggtggaag
     3181 aagggctttt tgtgggggcg gcggtcccgg aagccggtcc ggtcggagcg ggcgacctcc
     3241 cggcccaaga ggacccgctc ggaggtgacc accgctcgga tctcgatctg ggggtctgcc
     3301 aggtcggcgt ggtagcctcg cctccagagg accgagccga ccatcctctc cacctcgtcg
     3361 cttgccaggg gagccccgcc gagccgcttc gccctgaccc tgtaggatct atcgggaagg
     3421 tcgagctctg cggccgcctc ggccacgccc tccggagtcg gctccgcctc ggccgccacc
     3481 tccacgatcc ggtgggtcat agccatccgc gccccgaggc ggtcgaggga gaggtcgagg
     3541 tcccgagcct cgacgacgaa atggcgttcg ccctcggaga cgacccgata ccccggcgag
     3601 gtgatcgcca gcagcgcgag cgcctcgctc cgggggatcg tggggtgctc ccccgagagc
     3661 tcgaaggcgt atcggggcgc catctcagat ccggggagct tcgaggtagc ggttgacccg
     3721 tcggtagtcg aactcctcgg cgtcgaggac gttctccagg aagttgaaga gggccctccg
     3781 gacgtcgccg gtgaggagcg gataccaggt ggggctggcg acgacgacca tccgaaaggc
     3841 gaagaagggg gcgatcaccg aaagaagctc ctcgtccccc gttctgtcga ggtagttctc
     3901 gaagaagagc tcgaagaggt cagctaggcc gccctcgagg cggaggctct tctgaaccga
     3961 atagaagatg tagttggtgg tcatggcggt gatgtcgtcg gccgcctccc cccactcgcc
     4021 ccgggaccgg tctaggaccg agaagtccgt ccccgaccgg aacatcacgt tccaggggtg
     4081 gaagtcgccg tgagactggc agagccggtg ggtcatgttt cggagccgcc acctccagtc
     4141 gacgcacctc ttctcgatca aagcgagctc gcccggctcc aggaactccg ggtcagcggg
     4201 gtagtcgtcg aggatcccca tgatgcactc cccgtggccg acggtctctc ggatcttccg
     4261 ccggtagagg gcgggggagg ccttctttgc ggcgtggatc tcggcgagat atgtcgatag
     4321 aacctcggcc cgtctcatat ccagatccgt cgccccctcc tccttcaccc gctccaggtc
     4381 gaagaagtac tccttcccct cgatcgtctc catgaggatg aagaactccc gggcatcgcc
     4441 gcaggagctg agatccccct gggagctgaa gtatccgacg tccatcgacc tggcgtgctt
     4501 cgggaggcct ccgaaggcgg agtgctgcca gaggaggacc tgggcccggt cggagaagtg
     4561 gtcgtggccg aacccctcct ggacccgcat cgtcgagagg accgcagacc gggtctcgcc
     4621 gcccgtccga tactccagaa ggacggggct cccgtagccg aagcccttga cgtcctcgtt
     4681 ggtggggacg gtctccccga gccttctcat ccctaagagc tcagcgtctc ccccggtgag
     4741 gctctggagg tatgcctcta ccgcctctct cttcaggtcc atatccgtcc ccctcaggtc
     4801 cccgtctccc agctgtggag gtacttatcc tgctctgggg tcagggactc gatctcgatc
     4861 cccatcgccg cgagcttcag ggcggcgacc ctcttgtcga tctccgaggg gacggagtag
     4921 accttcctat cgagtcgtcg gccgttctcg gcgatgtacc ggacggagag ggcctggttt
     4981 gcgaagctca tgtccatcac ctccggcggg tggccgtagg cggcggcgag gtttatgagc
     5041 ctcccctcgg cgaggaggta gatcctccgg ccgtcggcca ttttgtactc caaaacgttc
     5101 tcctggacct cgctctttcc agaggccatc ccctccaggg ccgggatgtc gatctcgacg
     5161 ttgaagtgnn gtccttcatc agctcgacgt gctcttccct gatgaccgag acgttcccgg
     5221 tgacggtgac gaatagatcg cccaggggcg ccgccgcggt catcgacatc actcggaagc
     5281 cgtccatcgc cgcctcca
//
LOCUS       DASM01000037           32822 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_54522, whole genome shotgun sequence.
ACCESSION   DASM01000037 DASM01000000
VERSION     DASM01000037.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 32822)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 32822)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32822
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_54522"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    132..161
                     /estimated_length=30
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         6403 a         9817 c        10236 g         6336 t
ORIGIN      
        1 gtaaagttta tataacatta tgtaatttag gagttacatg ggtgggggag tgggggcatt
       61 ctctccaccc gtttgtatac tgggacggta gggatgaatg gggcaggttt cggacctgag
      121 gctggggggg gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncacgccccg cctccatttt
      181 cccgatatgt ggaagatgca tccggtccaa ttggggcgat ctatcgcaaa agaggctggg
      241 ttttagtatg gtcttcgatc ttggttcctg ggcgatattc gccgcagttc ttctcacggt
      301 gctatcgcta tccgcctacg ctatggcgga tgatatgacc gcaagcgact ggtttttagt
      361 gggcgaggat ctcctcgagg ctggatctta tgaggatgcg gcggccgcct acgacgaggc
      421 gatctccatg gatcccgaga acgggaccct tcacctctcc aaaggggatg ccctctatgc
      481 cctggccagg tcccagggct ccgactcccg gatcctcgag gaggcgatcg ccgcctatga
      541 cgaggcgatc cgcctcgatc cggcctccgc tatggccagg ctggggaagg ggagggcctt
      601 gagcctggcc tcaaacgccc tcgttgggga tgagcgggtc tccgggaaga acgaggccct
      661 ctccgccgtc gagatggcga cggagctcga cccggtcttc ttcgacgcct gggttttgag
      721 gggtaacctc ctcgacgagc tggcggccct caccggggat ctcagcaggt acaaccagtc
      781 cctttcggct tacgagaggg cgatcgatct tgcgcccttc ggcgacgcca ggagcctcgc
      841 cctggcctgg gatggcaagg ccgccgccct ctcaaccctc gccaacggcc ttgcggcggc
      901 gggggactcg gagggggcag cggaattgta cgaaagggcg gtcgcagcct acgacgaggc
      961 gatcgagctc gaccccgatt tcgccggcct ggaggcccgc ctcaacaggg ccagcgtcct
     1021 cgccggcctc ggcaggtacg agagatcgat cgaggcctac gaccaggcgg cagaagccgc
     1081 tcccggggat ctgaaggcgt acgtctccct cgtcttcgtc gagaaggcgg ccgtcctcct
     1141 ggagatgggg gaggaggagc tggcgctcga gtccctcgat gccgccaccc tcgccgaccc
     1201 cgggaacgcg gcggcctgga cgaggaaggg ggacctcctc tcggccctcg gccgcggctc
     1261 tgaggccgag gcggcccggg ccagggcgcg gggggtggag gagaagggat gatctccgca
     1321 ttttttaaca aattctacga ggtgaagggg ttgaaggtga atgagttgag aaatagctcc
     1381 tcgacggcgg ggttttgggc ggtcttcgtc ctcctgctgc tggccctggc cgcatcctgg
     1441 gccacggcat cggcagagga agggtccact gaaaagtcat cggagagctg gcgggatagg
     1501 gggcacgagc tcttctcgga aggccggatg gaagaggccc tccaggccta cgatgaggcc
     1561 ctgaggatag atccagggaa tgccaccctc tggctggata gatcgacgat cctcgacatt
     1621 ctggcccagc aggcactttt gagggctctt tccctcttcg acgatgacct ggcaaagagc
     1681 cccggagatg ccagggcttg gtgggggagg ggggtcgcag aatacggcct gggacggccc
     1741 gaggaggcga gggggtcctg ggagagggcc ctggagatct acaacgatac cctggaatcg
     1801 gatcccgagg acggcgaggc ctggtttgag aaggccgaga ttctgacggg cctggggagg
     1861 ggcgaggagg cgatcgaggc ctatgagagg gccatcgaga agaactccac ccgggccgac
     1921 gcggccgctt ttactgaagc gagcttgctg atccagaggg gacggtacga ggaggccctc
     1981 caagccatgg aagaagctac agacctcgat cccgagaacg gaatcttctg gcagtacaag
     2041 ggggacgtcc tcagggagct cggtcgcctc ccggaggccg aggctgcgta ttcgagggcc
     2101 cgagaactgg ggattgatcc cctagggggc atgatcgcca tcaccgaaat cgtggccgcc
     2161 ggcgaggatg atttcgtaga gatcgcaaat cacctcgacg aggccgcaaa cctccaaggg
     2221 tggaccctgg tcgtcgacgg ggatgagacg agatccgtcg tccttcccga gtacatcctg
     2281 gagcctgggg cgatggttcg agtccacttc ggcgcgggcg aggactccgg gaccgaccta
     2341 ttcatggaga gcgggatcgc cctcaacgac gatgccacca gcgtcaccct gagggacggg
     2401 gccggcagag aggtctcgtt tcttgggttt gaacgcctcc aggatggcgg agtcgtgatg
     2461 gtgaggggaa gtgggtagtc tgagcatcgg cggtgactcc agcagatatg acgccttgaa
     2521 gcctcgggat tggctttgat ccggggctag ctgccgcctc agaagagggt cgtctgtcct
     2581 gcagagccga agaaggccga cccctccccc ggcccgaaca ggaactcgcc ctcggggtcg
     2641 tggctcttcc gccggcggag ggcgaggtcc cggtggagga cggcgtagca gaaggcgcag
     2701 cctgcggggc aggtgtcgta ctcgccgatg tcgacggact cgtagcagcc gcactccggc
     2761 cgcttcccct tgatccgggc ccggatcggc cgcccaccga cggcggcgag ccttcgggcg
     2821 tcgacgcacc gggcggccgc cgccccggga acgatgtact ttcgctggga gcagaccgag
     2881 aggctcatcc cgtggcgcct cgccacctca ccgagctttg aggcgaggtc gagcttgaca
     2941 tcgtcggagg gatccatcca ggtgaagccg aaattgcggg ccgcaaggtc gaggttccga
     3001 gcggccttcc tgtagatttg ggcgaaggat atcaccacct cgtcggtcgc cccctccagg
     3061 gccccggcga ggctctcgaa gttctcgagg tggaactccg gcggggtcag ggaagagaag
     3121 acgatcgggt cgtaccgcca gaccgcggcc ttcggcccgt acgcctcggc gacggccctg
     3181 atctgccgga ccgccacagc ggcgccgacg gccccgggct ccagggcccg ggggtagcag
     3241 gttacggtct gctggacgag gaaggggcgg ccgaagtccc tcaccttctc aagccttctc
     3301 aggaaggggc cgaggtttct cgtccagaag acgaagccgt cgacgtcttc cctcgccaaa
     3361 ctgacgcggc ggacctggcg gctgttgtac ggattggcga cgaagcagta cccggcatcg
     3421 agccggtttt caaaccaatg gccatagaag gcggggatgt ctgtcttgta gctcgcggag
     3481 atgatcatct ttcgtcaccg atcgaggcag ccgctcttat ccatcgccat catcatcgca
     3541 tcttgcagct cttcttgtag tgggggcacc gctcgttggt gcagacgttc tcccgggtga
     3601 gggcgacgag ctcgacgtgg aggaggtcga gcctcccctg gaggtctggc gagaggtcgg
     3661 gaaagtcggg gccctctacg atctcgccga tccggtccct cagctccact gagcgcatcc
     3721 agtcgccttg gagggaggag aactcccgct ccaggtcgga gacgattttc tgccaggtca
     3781 ggggatcacg cttcatcttg tatctacttg gagctaaagg ccaaaaatct tatcagaaga
     3841 gctaaatcta aagagtaaat gcgcggagtc aaatcttccg cgcaaaactc agatcaaatc
     3901 aatgctttcg attttacgga acgatcgcca gtgtctctat aatggatata gtggtatcat
     3961 ttaaagatga tctcaagaaa atataattcc attcatcaag ataccaagag gcaaagtcgt
     4021 atcttccgcc aggtgtcgtt tgtgtcttct cgctaaaggt atgcactata acctctctgc
     4081 catagctatc gttgaccgag aaatttccga cttccttgtc acctctcagt tcgaaatagc
     4141 caatcatgaa tttctccata aacgttgatt gtagctgtgg atctggcttt tctggaacga
     4201 tgatgttcgc aaccaaaata gcagcatttc cagctgaagg gtcctcaacg ttttcgacga
     4261 ggataacctg cacgtcgtcc tcagcaacca acatattaga gatcgaaaag gccgagtcca
     4321 agtcgatggt caagtttgct tccttgacga tcaaaatttt tgtctcaaga gctgtgctgg
     4381 cgggcagcat cagagctaat cccaacacga cggccatcgt taccatcatt attttgatat
     4441 ttttcaaatc aaaccaccaa ttatacaggg aaactcattg tataaaaagc aagccttcta
     4501 ttcgttaaac caggtagttt gtccttcaaa tcttagctga tatctcataa caccttgaaa
     4561 tttaatactt cagttattcc tccaaaaaat aaacttgaag agcactattc atatcctcac
     4621 atcagtcctc agccccagaa tcttccgaac actgcaatag acgtctctcg cgatagaatc
     4681 tgcatggtgc aaagctccat cgccgcaaag agcgccggga gttggagaag ctccttaggg
     4741 gcggcgccca caccgccgta ttgaaggtga gggccgaggt ctgcctccgc tgtggtgaga
     4801 ggctctaatc tcgggaaacg atccggcggt tcgaggagat caggtcgaag ctgaggcgcc
     4861 aggagacagc ggggttcaag ccgatggggc tgtcgttcga ggtggtgtag ttggcgcgtg
     4921 ggcaccgctc gtttgtgcag acgttctcgc gggtgagggc gacaagctct acgtggagga
     4981 ggtcgagccc accctggagg tctggcgaga ggtcgggaaa gtcggggccc gctacgatct
     5041 cgccgatccg gtccctcagc tccacagacc gcatccagtc gctgcggagg gaggagaact
     5101 cccgctcgag gtcggagacg attttgcgcc aggtcagggg atcaccctcc ggttcatgca
     5161 aaacgagatt tcagatgcga tgatatataa atagataaga attgcggaat aatctccaaa
     5221 gatgggatac gtaggcaggg gacgtcgcgg aggcggggcg acaagctggt ggggaggatc
     5281 acgttcgaag atcctccggg ccaccgacca acgagggcga ttgtctggtt taggctcttt
     5341 gggccagagc cggctttcca caccttctca ccgagaggct cgagaaggtg ggggcgacct
     5401 ctaaggctgg acctggcggc cgccgaggga gaggtagcta cgacatgagg tgagcaacag
     5461 acgacattgt gtcagcttgg gctacaagtc cttgactgag gcagacccca aggcgaaggt
     5521 gatctattct ctattggctt taatatgtga ttttagctgt gaggcacgcc aaaggggaga
     5581 cgtcggttat caggttgtta tttaggttca gtttttgcag gttagtgagg ctcgctagag
     5641 gagagacgtc ggttatctcg ttgctattta gccacagttc ttgcaggtta gtgaggcctg
     5701 ctagaggaga gacgtcggtt atctggttgc cacttaggtc cagatgttgc aggttagtga
     5761 ggccagccag cggagagacg tcggttatct ggttccccac tagggacagg tattgcaggt
     5821 tagtgaggcc agccagcgga gagacgtcgg ttatctggtt gccacttagg tccagatgtt
     5881 gcaggttagt gaggtccgct agcggagaga cgtcggttat ctggttccta actagggaca
     5941 gatattgcag gttagtgagg ccagccagcg gagagacgtc ggttatctca ttccacccta
     6001 gggacaggta ttgcaggttg gtgaggcccg ctagcggaga gatgtcggtt atctcattcc
     6061 cccatatgga cagatttcgc aggttagtga ggcccgctag cggagagacg tcagttatct
     6121 ggttcaccca taggtttagt tcttgcaggt tggtgaggtc aactagaggg gagacgtcgt
     6181 ttatctggtt gtacggtagg tccagttttt gcaggttagt taggcccgcc aacggcgaga
     6241 catcggttat ctggttctcc cttagacaca gttcttgcag gttagtaagg cccgctagcg
     6301 gagagacgtc ggttatctca ttccacccta ggtccagtcc ttgcagttta gtaaggccgg
     6361 ccagcggaga gacatcggtt atctgattcc tccataggga cagattttgc aggttagtga
     6421 ggcccgttag aggagagacg tcggttatct ggttcaccca taggtaaagt tcttgcaggt
     6481 tggtgaggcc agccagcgga gagatgtcgg ttatccggtt caatcttagg tacagatatt
     6541 gcaggtttgt gaggcccgct agcggagaga tgtcggttat tccggtgcta cttatgtcca
     6601 atttttgcag gtttgtgagg cccgctatag gagatacgtc agttatcggg ttccacccta
     6661 ggtacagata ttgcaggttg gtgaggcctg ttagaggaga gacgtcagtt atctcattcc
     6721 cccataggta cagttcttgc agattagtga ggcccgctag cggagagacg tcggttatct
     6781 cattccaccc taggtccaga tattgtatgt gattacaata ctccagcccg gcaagatctc
     6841 tgatatcact tttattcgcc tcaaaacgat cgatctgttc tagatctgaa acatagatgg
     6901 tgccttctgg tttatcaatc gtgtctctta cggcatcctc aaggttcgga tcggggaata
     6961 cgacttccgt ttccacctca tccgccacgg ctggcattga tagcgatgct agcattagca
     7021 gaatgtaaat tagcttcagt ctcatcttat tcaccctatt agttagtgtg aatgacacaa
     7081 agtaaaaatg ttcctatgag ttgtcttatg taaggaacgt atcgaatagt acacttatgc
     7141 aaagtcggcg aaattctcca aaaaagccaa gtttacgcca gctaatttat gtgactcagc
     7201 tacccccgca ccctcccatc caccccgatc cccaaaacct ccctcacccc cttgtacacg
     7261 tcctccgcga tcagcgcgca ccccagcgcc gcatcgcagc ttgcgagcgg cagaacctcc
     7321 cgccccagaa gccccgagac cctgcccgcg atccgggagg cgggatcgcc cgctaggtac
     7381 agcgccggtt tcgctgcgcc ctggtcccgg aggaggacgg ccatggcggc gagctccatg
     7441 gcggcataga gggccagcgc ctcgaaggcc gcctccttct cttcgggggt gcatctcttg
     7501 agggtgaggc cgcgcttccg gaggataccg ccccgggtga aggcctcgtt ggcggtcatc
     7561 ttccctgcat cgacggcccg gatcgcggcg acgtctagcg gcccatggag gagccccggc
     7621 gcgaagatgg gggcgtctat ggcgccggct atgcgcccag cagcgacgcc gatggtgacg
     7681 acgttggagc tggcgtcgga gatgatgaag tccttcttgc cgtctttgaa gaccttgtag
     7741 gcgagcccca ccttctcggg gctggcgccg tgggagaaga ctttcatccg tgggtcgatc
     7801 tcggagcggt cgtgaattcc tgggaggagg atcgcgggcc atttcgccgc caggacggcg
     7861 tcgaagacgt tcgttccgcc gccgacgtgg aggccggcgc cgtcctggct gatcactccc
     7921 cggttctcgg cgtcctcgat tttgacgatc tcggtgaggc catcgcccat ggagtagcag
     7981 agggcgagga gatcgatctt ctcggcgccg gagccggggg cgaggtcgaa ggccccccgg
     8041 atccggtcga tgatctcccc gggggcgagc ctggccgcct cctccctcgg gatgacgagg
     8101 catttgccgt cggagtctgc aaacctcatc gccctcgtcc cgtgatcgac gcctatgaac
     8161 atcctacacc gcctccgcct caatccccgg ctccatctct tcatctgcga tcctgacgat
     8221 ctcctccgcc agcttcaggc tgtccctctc gtccctcatg gtcgtgtcca gccggacgga
     8281 ggggccccga tagtcgcttt ttgggctgac caccatcagg tcgacgcacc cgaggatctc
     8341 ggcgaccccc tggtcggtga cgggaaaccc ccaggcccgc atgaacttcg cggcggggcc
     8401 gctgacgggc cgcccatcga cgagggggct gatggcgatc acccggctcc tgctcctccc
     8461 cttccccttt ctcctgctgc tccgacctcc caggaggtcc cggacccccc ggagggcgag
     8521 gatcggcccg atgctggaga cggggttgct cggcccgatg aggatcggct ctcccacctc
     8581 ggccgccctc tccagggcgg cgaggaattc gaggcttggg agggcgtcct cgatccccct
     8641 gaaccgtatc cccgtcacat cgggtcggcc ggaccggccc acccagaact cctggaggtg
     8701 gatctccccc tccggggtcg ataccatggt ggagacgggg tcgtcggtca tggggacgac
     8761 ctttcctgcg accttcaggg caaggcagag cctcgccgtc gcctcggaga gggtgagccc
     8821 ctcccggagg agctcggacc ggaatatatg gatggagcgg tcgaggtcgc cgagggcgag
     8881 gagctctcgg cggccgaggc tcttcagaaa atcgtgggtc ctaaaagtgt ccccctcgat
     8941 cccccaccac ctcctccggt cgatcatccc cgccagggtg tagacgacgg tgtcgaggtc
     9001 cggcgagacg aggtttcctg agatccagag gtcgtcggcg gtgttggcga cgaccgtcag
     9061 ctcctgggga tcgagaacct ccttcagccc gacgaggagc ttgggggtcc ccgtcccgcc
     9121 gctgaggact atcagtctat ccgcctccct gggcttcgcc ggtcaggcgg cggagtcgag
     9181 gatgatcatc ccggtcttca gatcctcgca ctcctcgggg gcgagcctcg tcgagatcgc
     9241 cttctcagag acgatggcgc tatttcccag ggggtcctcc aggatcaggg tgagggggcc
     9301 gaccccctcc atcgcccggt cgatccgctc gattatccat tcgccccggc agacgttctc
     9361 ctccgccccc gcgtcccggg cggaccgggt ggcgaagagg acgatatccc ggaccctctg
     9421 gaggacgccc tcgacgttcg atatgtaggc ctcggaggcg gggccgggct ccacgtcgat
     9481 ccccagctcc ggaagcctga tcgtcccgct ggtggacctg atcacccggg cgttcaggtc
     9541 ggccacctcc tcgatggtga tggcatacct gacgggatcg ccctggctga ggaggatggt
     9601 gtcgctgagc ttgaagccgc aggcattgca gaccccggcg atgatcagcg cctcgccgaa
     9661 gtgggggatc tccttagtct cccagtcgaa ggcgatctcg gcggagcaga tcgggcagtt
     9721 tgaggtggtc ttcgccttca atcctccacc cccaggacgt cctcccgatc tatcctcatc
     9781 cgccggggga cgactatcac caggttgtcg ccgaggccgg ctatgtcccc ccttagatcc
     9841 tcgacggagg ccttcaactc cccgatcgcc tcgtcgagga ggtccttctc cctcctcatg
     9901 ggagatatgt ccaccaggac gaggttgccc ctctctatct ccgtctttat ctcggggagg
     9961 acctcgaggt cgttcagctc cgccatcttc accaggatct ccgaggggcc cccgccggag
    10021 acctcctcga actgccccag gtctatgttc acgtaatcct catccccaga cctgcccatc
    10081 aacttgtcca agatgcccat atctcgacct ctagcaggag gtatgcagcc tcttcaaatc
    10141 catctccagc cgcctataac tcccccgtct ccatctcagt ccatccttcc agcctcaggc
    10201 ctcaattctc aaaagcccga accccgctag ccccatcagg atcatgaagg caaggaccgc
    10261 gatgacgaac cccgccggcc gaaggggctc gggagcctcc gtctgaacca ggatcaagag
    10321 cagtatcatc aacaggacga gcccggagct ggcgcatatc tcaagggcct tcttattcat
    10381 accgcccccc ctttgctctg ctctcagata tcatttgcga ttcggacctt ccacccgagc
    10441 cctcctccgg aggccgtcgg gccccgccag ctcttcgacg agggcccagg tctttctccc
    10501 atgccaccgg aggcggatgt gcctagggta gaccgggagc ctctccagaa gatcaaagcc
    10561 ggggagccgg gctttcagct ccatcagggt cggccaccgc ctctccgggt tgatccagtc
    10621 cggggtgacg gagctgatcc cgcccaggtc gtccgccccc gcctccgcca ggaggagggg
    10681 atctgccagg tttgggggga cctggacggc gacctccggc ggcaggatcg accgagccat
    10741 cctcaccgtc tccgccagga tctccggccc tggcgctggg gccccagaca tcgccgtccc
    10801 cggcttgggg tcgaagggct ggacgatcac ctcctggatg tggccatgag cctcgtgggc
    10861 cctccttatg gcatcgatcg atcggagccg gtccccctcc gtctcgccga tacccaccag
    10921 gatcccggtg gtgaagggga tcttcagctc ccccgccgcc gccatcgccg cgagcctcaa
    10981 tagaggcctc tttcccgggg aggatcggtg ggcctccacc tcggcggtcg tctcgagcat
    11041 cagccccatg ctggcgttgt agggcgcaag cctcctcatc tcctccttcg agaggaggcc
    11101 ggcgttggtg tggggaagga ggcccctctc cagggccagc tccgatagct ctatcaggta
    11161 gcctatgagg tcgtcatacc ccgtcccttt caggatctcg gcgaagccgg agacctccca
    11221 gggcgcctcc cccagggaga agagggcctc agaggagccc gccgaggctc cccggtcgag
    11281 gaggacgatc gcctcccggc ggccgatgat cctcccccga tcccgccgga agctgcagta
    11341 tccgcaccgg tttctgcaga tgtcggtgac cggtatgaag acgttccggg agtaggtgag
    11401 aacccctctc atgaaggctg tccggaatga aaataggagg atggggggcg gggggatctc
    11461 agcgcctcag ctccaccagc ttcagcttgt ttccgatctc gcactcctct ggggcctcgc
    11521 cgacgaggtc gacgatggtg caccggtccc cctccttcag gcctacgggg tggcagagct
    11581 cgtacatggt gcactcgtac ctctcacacc tgggcggctc caggacggtc ttcgacccgg
    11641 cgaaggcctt cctcgcgtcg atggcggcga tgatggggct ctcggtcacc tccaccgccc
    11701 ggacccccgc ctcgtggatg gggcagtcgt ggaccagctc cccccggatc cctaggatcc
    11761 tgtacctcct ccccgactcg aggttggtgc aggtgttcct cagcttgcag gtctcgcatt
    11821 ccggagaggg ggcgttgaag atgaactcca tccctatagt cgcgagcttg gttcctatca
    11881 gggtgatctg tgttgctgct tcggacatgg tttctcctca aatgaataat tatattatct
    11941 tggtcttctt ggctgctgcg gctgctgcct ctcgggtgag ccccgagccg aggatggtat
    12001 accgctcggg gcggatggtg tgggcggagg tgagggcctc gatcaccgcc tcctccgcga
    12061 ggccgatatc ggcggctgag gtgggggcgc ccaccttctc cagggcatcc cggatcatcc
    12121 tccagtcgcc gccgtggagg tacatcatga ttatggtccc aaggccgcag agctcgccgt
    12181 ggagggcctt ccccggttcg atcctgttta tggcgtgggc gaacttgtgc tctgagccgc
    12241 tggcggggcg ggaggagcct gcgatggaca tggcgacgcc gctcgagatg agggccttga
    12301 cgacgaccct cgccgactct tcgagccccg gcttcagctc cgaggaggtc tcgacgatca
    12361 tcctcgccgt catctccgat agggccgagg cgtactcgct gtactcctcc ccccggaggc
    12421 ggtgggcgag cttccagtcc ctgacggcgg tgatgttgga gattatgtcg ccgcatccgg
    12481 ccgccaggag cctcttgggc gcctgggaga tgatagcggt gtcggcgacg atcccgaagg
    12541 gggcctgggc ggagacggag gtcgtctcgc cgtcgatggt gagggaggcc tgggcggagc
    12601 agataccgtc gtgggaggcg gcggtgggga tggagacgaa ggggacgccg accctgacgg
    12661 aggcgagctt ggctacgtcg atggacctcc cccctccgac ccccacgaga aatcccgccc
    12721 ccatatccgc cgccatccgt tccgccctct ccacctcctc gatctttgaa aattttgtga
    12781 tcatcagggc cgtctcgaag ccgccctctt ccagccgccg ggagatctct ccgccgacga
    12841 gctcgcaggt tctggggccc gtgacgatga gggcaccgcc cgcgagtttg aggtctctgg
    12901 agatctcggc cacctctctt gcgacgccgc tgccgacgac caccagccgc ggaagctcca
    12961 tccacttcga tctcctcaac ggtctcacca gctcaagaca aaattcgtac ctctacgaat
    13021 ataaacgctc gcaaaagggt tccctcagcc ggtccggatc ggtccactcg agatggaagg
    13081 cccctcaagt cggcggggta tggtgagatg gaaggattgg caaacctgaa aaaatcctca
    13141 atcctttcgg aggctcttca gaacggagag gacctgctct cggtacctgt tcacctcggc
    13201 gctggtcctg acccggggcc gggggatctc gacgtcgatg atctcgctga tctggccggg
    13261 gcgggcggac atgatcacca cccggtcggc gaggtagatc gcctcgtcga cgctgtgggt
    13321 gacgaagatg acggtcttct gctgctcctg ccagatccgg aggagctccg cctgcatgac
    13381 gttcctcgtc tgggcgtcga gggcgccgaa gggctcgtcc atgaatagag cccgcgggtt
    13441 gttcaccagg gcccggacta tcgcgaccct ctgcttcatc cccccggaga gctcgtgggg
    13501 gtagctgttc tcaaaccccg caaggcccac cgccgtgata taccggcggg ccttctcgtg
    13561 ccgctccgcc ttggagacgc ctttcagctc cagaccgaag gctacgttgt cgaggatcgt
    13621 cctccaggga aagagggagt actcctgaaa taccatcccc cgctggggcc ccggcccctc
    13681 gatcggctcg ccgtccagga ggaccgcgcc gctggtgggc ctctcaaggc cggccatgat
    13741 ccgcagaagg gttgtcttgc cgcagccgga ggggccgagg aggcagatga actccttgtc
    13801 gtagacggag aggttgacgt cgcggagggc ctcaaagcgc ttccccttca ccgagaagct
    13861 cttcgagagg tccctgatct ccaggagggc ggccatagtg gcgggggttt ggtcggcgga
    13921 ggtataaggc ttttctgtcg gctgcctatc acgtgctacc agcggcctcc gaggcctttg
    13981 atctccgact ctaatcggcg ccattgccga tcatccctca cctcacccct gcacctcccc
    14041 gtcgatcccg aggatgacgc tcctcaccgg cacctccttt cccgccctct tgatagcctg
    14101 gcggacgaag gcctccaggt tcgagcagca ggggacctcc atctccaaaa cggtgatatc
    14161 ctcgacttcg tgggcggcga ggatcgcggc gagcccctcg acgaagctct ccctctcgtc
    14221 gagcttcggg cagccgatga gggtcgtcct tccccggatg aagtcctggt ggacccgagg
    14281 cgaggcgaag gcggtgcagt ctcctgcaat tagaaggcgc gcgccgtcga acgaaggcga
    14341 gtcgacgtga gcaagcctca tctggaccgg ccagttggag agctccggct ggcctcccga
    14401 ggccttcgaa tcgccctcgg acctgatgcc ggtcgcagat cccgaagccg gggcgcggct
    14461 ccgcctcccg gcgagcttcg aggagccgag ccttcccgcc cccgtgcccc tcagggccat
    14521 cgggcagccg ccctccccct cgccggggcc gtcgccatcc tcgcagccca tcgattgccc
    14581 tgttgatcga gcccggtggg ccggttcgac cgccgccttg gcggcgtctt cgtcgaactc
    14641 ctccgcctcc cgctcctcca tcgatagggc gcccgttggg cagtctccga tgcaggcgcc
    14701 gaggccgtcg cagaggctct cccggacgac cttcgccttt ccgccgacga tctcgatggc
    14761 gttctcggga caggcgagga cgcagtttcc gcacccatcg cagagagcct cgtcgatctt
    14821 tatgatcttc cttatgacca ccttcatcca cccctaaccg acgccagtat tcggctcacc
    14881 agatccatct ccacaagtat aaacgtcgac gactcaaata ggcttcgaag aagatttcga
    14941 tagctttcgt ggaggctgaa aagatgaaga tcgtcgaggt ggagaaggcc gatagggcgc
    15001 caaaccccca cggggtcgat gcgaggatga tcagcgatac ggaatcggcc caggtggtcc
    15061 acatcaccct tgcgcccggc caatccctca agaagcacat cacccccgtc gacgtcgtct
    15121 tctacgtcct ggaggggagg gggatcgttg agatcgggga cgagaagagg gaggttactg
    15181 ccgactccct ggtggagagc ccggcgaaga tcccccaccg ctggaccaac ccgggcgatc
    15241 ggcgtctcag ggtcctcgtc gtcaagaccc cgaggccgac ggagcagacc cggatcctgt
    15301 gagggtggtt ccgggggcct cggcgatccg gccgccgaga gccttttttt agctatcagg
    15361 ccgacccgac ggaccgacga cgtcggaaga ttcggatcta cgacttatcg gccttttcgg
    15421 acttttcgaa ctcctgctgg acgtcgcagc cgagctttcc gatggcgtcg gacctgcact
    15481 gtcggcagtg cctcatctgc ttcatcacct tggcgagctc ctcctggacc gccctcttct
    15541 cggccggggt cgggggcgat aggtgggcga accggtactg ggggatcagg ggcatcacgt
    15601 tctggatgta aaccccgagc ctcttgatcc tctctgtgat atccatgatg tggccgtcgt
    15661 tgatcgtcgg tatcaggacg gtgtttacct tgacgatcat cttcctcctg accgcctctt
    15721 ccaggcctct cagctggtgg tcgaggagga gctcggccgc ctccaggccg gtgtacttct
    15781 tgccggcata gatgacgtgc tcgtagatct cctttcctat attggggtcg acggcgttga
    15841 gggtgacggt gacgttgctg acgccgaggc cgtcgagccg ttcgatctca tcggggagga
    15901 ggaggccgtt ggtgctgacg cataagatga gcttcggata cttctccccc accagcctga
    15961 aggtctcaaa ggtcgcctcg ttgtagaggg gctcgccggg gcctgcgacc gccaccacct
    16021 tgatgtagtg gagcttctcc atcacctcgt cgacccgctg gagcgcctct gcgggggtca
    16081 ggacctggct cgtcaccccg ggcctcgtct cgttgacgca gtcgaagtcc cggatgcagt
    16141 agttgcactg gatgttgcat cgaggggcga cggcgaggtg catcctgccg aaccggtggc
    16201 aggccttctc gctgaagcag gggtgctcct ggatcctcct cagctgctcc ggatcccagg
    16261 gaacctcctg gtcgccgacc ttggcggtgg gataggcatt ctcgctcatg atcacgaact
    16321 ccagagatcc tttcggcttt tcttctctaa aaggatatct gaaggttcgg aggtccctgc
    16381 cgccccgccg cctcccccgg atcggccaaa aatcttatcc cgcaggggag cgtcgagtag
    16441 actcatgtcc agcaaagacg gccgatgggt gatctggccc gcctacatcg acgccgggag
    16501 gtcgaggggc caggggaggg cggtgtcgag gcgcgatgcc gtcgaggagc ccaccgtcga
    16561 cgagatcctc gtagccgcct tggagatggg gctccaggcg gaggcggaga gggataagca
    16621 ttaccctaag gagtggtggg agaagtccgg cagggtcagg gtccagaaga aggggccgaa
    16681 ggccgctctg gtcagagaga tcgccactag gatccgaagg cgacgaggct gatccggggc
    16741 gatggacttg aggcccatct gaaagatgcc gctcagaccc tagaggtccg tcctccgggc
    16801 ctgacctcac cggtcctggg cggtctccac cccttctaca atctccttcc ctgccacgac
    16861 ctcgtcaatg ctgtggatta cgccgccgaa gtcctctatc accgactcca cagccaagta
    16921 gtcgatctta tcccccttga tggtgatctt gatcgtctcg gtctgctggt ccacctcgga
    16981 gaggctgagg ttcaccccgg ttatcccgcc gagcttgctc agagcctcgg cgagctcgag
    17041 gatggtgggg acatggggct tcaatacgtc aagtaccaaa cgtctgatgg cggtggtcat
    17101 cgaaaactcc cgtctttatc ttgggcggat ctcggtttga ggcaatactt aataatgttt
    17161 gtgattcagg caagctcatg atcgaccttc acacccatac cgtcttcagc gacggcgagc
    17221 tgatcccttc cgagctggtc cggcgggccg agatgaaggg gtgccgcgcc ctggggatca
    17281 ccgatcacgt cgacttcacc aacgtcgagt tcgtcatcag ccaggtctcg aaggtcaagg
    17341 cgctggagga tgtcctggag atgaggatcc tcgccggggt cgagctgacc cacgtccccc
    17401 cggcgaagat ggagcggctg gcgaccctgg cccggaggat cggagccgac ctggtcgtcg
    17461 tccaccggga gacggccgtc gagccggtgg cccccgggac taaccggaga gcgatagaga
    17521 tcggggtcga tatcctcgcc caccctggct tcatcacccc cgaggaggtg gacctggcga
    17581 gggataacgg cgtctgcctc gagctgaccg cccgccgcgg ccacaacatg accaacggcc
    17641 acgtcgcccg cctcgccctg gaggcggggg cgaggatcgt cgtcgacacc gacgcccacg
    17701 cccccgagga cctgatcgac cgcgagaggg cgatcgagat cgcccggggt tcggggctca
    17761 ccctggagcg ggcgacggct gtcgtcgaca aacacgccat catcgacctc tgacgaagag
    17821 atggtacctt ggacgtggtg gccgtcgaca tatcggggag gcacgccgtg gccggaagat
    17881 acatcatggt ctccgcctcg gtggcggcga ggatatcccc ggagggggtt gagaggacga
    17941 ggaggatgag gctcgtcccc cgggtggcgg agtcggtgga tatggaggtg gtgatcggcc
    18001 tcatggagga gtcggtccgg gggctctccg gcgtcgtcct cgccgagagg ggcgacctct
    18061 acaacctcga gcggtggagg gtcgagagca tcctgggacg ggagttcaag taccccgagt
    18121 cccgggggga gaggagggcg gtggagctcg cccaccacgc ctccttcgcc ggccggaggc
    18181 tggccctgag gctgatggat gaggggtggg agtcggttga gggatgaggg tgggaggtcc
    18241 atgagacggg agaagacggc ggtcctcctc ttccgggagg acccgaggga agacctccgc
    18301 ccggaggagg tgaccgagct ccgggggctc gccgaggcgg cgggatacac cgtcctcgcc
    18361 gaggtattcc agaggagggg gagggaccgg aggtttcagc tcgggagggg gaaggtcgtc
    18421 gaggccgcct ccctcggtcc tgataagctg atcttcaacg agcccctcag ccccggccag
    18481 atcttcaaca taggaaagga gttcgagatt cctcccatgg acaggttcaa cctgatcctg
    18541 gagatcttcg catcccgggc ctccacccgg gaggcgatgc tccaggtgga gctcgcccgc
    18601 ctctcctacg aggccccctt cgtccggacg atcgagtccc tcaagaagct gagcgagcgg
    18661 cccggctacc ggggctcggg gagctacgac gtctcgatgt accgggacat caaggggagg
    18721 atggcgaaga tcagggccga gcttttggcg gtggaggaga cgggggtccg ccggcgggag
    18781 cggaggagga agctcggctt cgatctggtg gccctggccg gctacaccaa cgccggaaag
    18841 agcaccctat tcaaccgcct cgccgaggag acggtggccg ccaaagacca gcccttcacc
    18901 accctatcgc cgacgacccg ggcggtggcg gcgcgggatc ggcgattcct cctcaccgac
    18961 acggtggggt tcatcgacga cctccccctc ttcctgatca aggccttccg gtccaccctg
    19021 gcggagatcg tcgaggcgga cctcgtcctg ctggtggtgg acctctccga tccccccgag
    19081 gttctgaggg agagggtcgc ctcctgccac gaggccctct gggactgtgg ctgctacgca
    19141 cccctcgtct cggtcctgaa caaggtcgat cggatcaccc cggaggaggc cgaggagagg
    19201 tgcaggctcc tggaggacct cgcccccacc cccgtcctcg tctccgcccg ggaggggctc
    19261 ggcctggagg ccctcctgga cgccatcctg gagaagctcc ctcccctgga ggagttcctc
    19321 gtccgcctcc cggcgtcgga ggcggggatg gggcagctct cccgcctcta cgaggtggcg
    19381 gagccgctgg aggtgaggta cggggaggag gtcgaggtcc tcctccgggg gaggagagag
    19441 atcgttgcca aggccctgag gggggcggag aggccggagg cggatcagga tatggacgat
    19501 cggtccgggg cggccacggc gccggagggg cggcggccga aaccgggaga tccgcccccg
    19561 gatggggaat gacggggatc ggcccctcga agggatgatg gagagatgac cgctaagaca
    19621 gataagacgg ataagacggt ccttctgagg gagaggttcc agctgaagga gacgatcgtc
    19681 accatcacct tccaggagcg ttcccacctc gaggcagcga aggaggagat agcgatccag
    19741 cgggccctcc tcgaggattt catccgggcc gaccccttct tcctcgtaac cctggagccc
    19801 tacgaccttt cgaccggcga ggcgcctgag atcgtccggg agatggcgag ggctggccgg
    19861 gccctcggga tcggccccat gagcgccgtc gccgggacga tagcccgctt cgccctgggg
    19921 gcgatgatgg aggcgggggc gacccacgcc atcgtcgaca atggcgggga cgtcgccctc
    19981 atcgccgacc ggcccgtcct gatggggatc tacgccgggc catcccccct gaaagaccta
    20041 gctttcgaga tcgccccccg gacggagccg ctggggatct gcaccagcag cgggaccgtc
    20101 ggcccctcca tcagcttcgg ctccgccgac gcggccgtcg ccatctccga ggacgtctct
    20161 ctcgcggacg ccgcggcgac ggccctcggg aacgccgtag gattggcatc accactggcc
    20221 gactccttcg gtgcggtggc ggttccgggg gtggcggggg ccctggtcat ccggggggag
    20281 gagatggctc tctgggggga ggttccgccg ctccggcgag cgagcctccg gcccgagctg
    20341 atcaccaggg cttaaaagcg aaatcaaacg atgagctttc tcgcgtgaac gtccatcaaa
    20401 cgcggataat tgatctactt cgcgatgcgt tctcggactt cttcgaattc gtcgaggagg
    20461 tagtatactt cgactgcgga cctcctggca ctttcaacct gatgtttatc gtttgagacg
    20521 agaaatgctt cttcaagctt tgcagcagct acgtaaaaag aatcgatcgc ccttgatccg
    20581 gtcagaacag caacactata ggccatatca aaaagttccg atgcttcgat gaagttaatg
    20641 ttggagaaga tctcctcggc cagagctgtc gcctcttcct tcgtgagcct tctgacgagc
    20701 tggccgatca tctcaatctt gaatgcgtca ggctcgaaga ttggaacgcc atcttcctcc
    20761 atcaaggtga agaggccgtc ggcgatggca gtcctatcgg agcgataatc gaagatcaga
    20821 tccaggaaga ccgacgcatc gacgacgatc atcgtctctc ctcgacgaac tctttgagca
    20881 ggtcctcgtc gaccttcctc ctgtgcctcc tggcagcctc caggatccct cccggcttta
    20941 tcaccacgaa ggcctccgtt ccctccttca gcttcacatc tcccagcggt ctgaagacac
    21001 ctccctcata gacgacctca atcttcttat cggccttagc ggtgtagact gcgcccatcg
    21061 ttcagacccc aggttatatg ggaagttgaa ggatatatag ccccctctta tccatgtcgg
    21121 atagaggtcg atctcaatat tttctcttcg tacggccctg gggtggccgt ggatcgacca
    21181 cggcgcagta tccggagcgc ttccctcctc tccaggaggg gcgattcttt ccccgtcgcc
    21241 agaatccttt taacatatta aaccataatc gacgcaatat ggcggcaaca aaatcgaagg
    21301 ggcacgttat gggctcgaag ataccagcca ccgagtttga gcagatccag cgattggtgg
    21361 aggctggggt ctatctaaat ccatccgatt ttgtgagaga tgcaataaga gataagctcg
    21421 catccatcaa ggtgatcaaa tatcgggatg tggattacaa taccgcaaag aaagaggttt
    21481 cagggtactt ccaatccagg ggtgaggctt acccctccga cgccagcgaa gatctggagc
    21541 tggactacga cctcgtcgcg gagatcaccg aagagctccg acgggagggg cggcttgagg
    21601 tcatctgatg gaaggccgcg aggtgggaga ctccatcaga gggtcgagga tatatgcgga
    21661 ggcgaggaag atcagcgatc cggtcgtcat agtaaaaaca gggcggcaca aggcccagcg
    21721 agtcatctta tcagagaacg ccgtggcgag ggtcgagaag aagcttaggt ggattgcgtg
    21781 accgaaggcg cagggcaccg gctcaccgat cgccgccaga aagtagatct cgaagaaacc
    21841 tctgacctgc ctcttcgaga tcgccatcca aggaaaatcc caggctccga accctaattt
    21901 tgccgatcgt cacgtccaag tctaggggct tgggatagat cagcaccgcc tcatcgcagt
    21961 ctttggcggc tgcgtaggtc accacctggc tgatgtcgtc gtttcctgga tggtccgggg
    22021 ctttgtattt ggtgtcgagg acgaagagcg ccttctgcga gtctctatcg tagaccacca
    22081 gatcgatgtc gaacctgacc ctcccccctc ggccgatgac gacctcctca tggggtctga
    22141 ccacaaaatc cggcggcatc ttcgctttca gccattcggc gacgaagagc tcgtagagcc
    22201 gggccatatc caccaggaag gggaccatcg tccggcctcc gatccggtga gacggtccgc
    22261 tctgctccaa aaagaagcgg catagggcgt gcatcggctg atagtcctcg ttcaggcggc
    22321 tgtaaagccg gccagcgcaa tccccggggt cgcacggaat cggggttgtc aagcccctaa
    22381 gagactggta agccctccgg acctggggga gggatcgctc ggtgcagacg ccgctctgga
    22441 ggattcgccg gagggtccag gcgaggatct ggttatcttc gacgtcggag gtgtgctctt
    22501 cgtaggagca ctctggcttc accacccatg gtttctggca gctcttccgg aggtcgaagc
    22561 ggccgcggac gtaggggatt ttgtctcttc ggggctgata agatcgaaag aagcccttcc
    22621 gcccccggtc gaggacccgg agggcgagga agttggccag ggtctcatag aactcttcga
    22681 gagaatcgca gtccgttaag ccatcgagaa acttgaggct cttcctattg agccagtagg
    22741 cgtactccag catcccgaat aggttcttta tatccacctt cggcaatagg gcgatctcca
    22801 gctctggagt cagaggtatc tgacccgccc agccggttgg agttaaacac cactgattgc
    22861 cgttcttgaa ggacggctca tctatcgaga tctggctccc atagctctcc catatcttca
    22921 gaccaagctc ttcggagatc tcgactttgg gaaacctccg ggtctcgtac tctttcagct
    22981 ccatccgccg aatttcgttc gatctcatgg agcgatctct ccgcgcacct tatcccaccg
    23041 gaactcttcg gccttcgccg tcctgtcgaa gaagtactcg tcgaggtagg gctcgacctc
    23101 catcttccag atgtcctcga tctcctcggc gaggtctttt cggaggaaga aggatatgcc
    23161 gacttcatag tgggggtcat tgacctgggc gttcaggcgg cgtaagaccc caataagccc
    23221 ctctacggga aagcccgtat tccgactttg gtgatacttg cgcagcacct cgtgatttgg
    23281 ctggaggcgg aggaaggcga agcgccgccg gagggcgtga tccaccaatg cgatagaacg
    23341 gtcggcggtg ttcatcgtcc cgatgatgag gacgtttttg gggatcctga gccttccgcc
    23401 tccggctagg gggatcgaac ggctcctata ttcgaggagg tacatcaatt ccccgaagac
    23461 ctgggagagg ttcgcccggt tgatctcgtc gatgatcaga acgcaggtcc ctcgcctaga
    23521 atcggcccgc tcgcagaact ccaggaaccg tcccgggacg agggggtacc tcaggccgcc
    23581 gtcgtcgtcc gactggggcc ttatcccctg gatgaagtcc tcgtaggcgt aggcgggatg
    23641 gaactggacg aggtcagaga agccatctgt cccgccgatt aggtgctttg cgagatgctc
    23701 tgcgacgtaa gtcttccccg tccccggcgg gccgtagagg acggcctgtc cctttcgtct
    23761 gatggcgcgg acccagcgct ctaaggtttc gagatcgaag cctgtgtctt cggcaagttt
    23821 gtaaagggga tactcagagg gtggtgtcgg tcccaagaaa tcgggccaat cggtctcagg
    23881 ctgagatcgt cgaattatat caagcccctc caaatttagc cgaaataggt atccctgttt
    23941 tacgccacca ttgttgttca gagggccttg atttatatcg agtttcgaga gcgattgtga
    24001 gaatttgtcc aatgggatgg ggggctttag ctcatgataa tctaccttaa cgaggttccc
    24061 atcctctgac caatttcttc cgtctatggc gtgcggatat ctagtccttc ttgatggttc
    24121 gataactcgg cttgcataac gcaagcatcc gttagcataa tgaaggatta tatcatcttt
    24181 gacaagatct gacaggttat cccaatgata aaatttacgt cctgtttttg actttgttgg
    24241 ggcccacaga taaccgccat ctagttctaa atcaagatta gacccctgat ttacccacca
    24301 aatatttgat gtttttgggg gcctgaccgc cagccagtgg gtaaacatat caaataggtc
    24361 atcaaatctg atacctgaga tcttaaccct gtcagtatga tctgagatgt cattaaccag
    24421 tgaacgtgcc gtctcattga tcgaggggta atctgataga cttgacgagt aagaggtgcc
    24481 cgagaagtaa ttgacagcac gcctcgattt gttgttgatc aagatgaaat cttccggcct
    24541 tagggcgtga agaatcggcg tcagagtacc agattggaac cctttggagt aaggcgaatc
    24601 cgagaactca tcacatgcgg ccttcagctc ctcgggtcga tcgtagcagc ggcggacaaa
    24661 acggaggatc tcttttgcga cttgaggcca tgattcttga tctgtccatc cggcggattc
    24721 gaacttcttc ctcacgtcac cattgaaggc aggggcgatg gatatccaaa agcccctctc
    24781 tcgattgggc tttgagcctg cataggggag aagcttcatg aggacttcat cggttacatc
    24841 ctcgccccgc ctttccgctt cgaggatcgc ctcaaaattc ctgcgacctt cttccctctc
    24901 ctcttcgtaa tgcctgagat gatcctcgcc ctgaggtgtg ggcggatagc tgtctataaa
    24961 tttgcgaaat agctcaatga actcggccct cttctccgcc agcacgtcaa atctcctcca
    25021 tgtttatatc tgaaatcaag accataacct cgaagtacga cttgcaggat atgatgatgg
    25081 catctgcgaa aaatgttttc ctgaatccga cggattcaag tctttcaaag gcacgggtgc
    25141 gatagcggtc tatggcgcat tttgccttga ctggaatcga ttctgggcta atatggggcc
    25201 ctgacgacct ttttctgatt tttcagtcat ccttccggct ggtagttatt taaatcataa
    25261 gaaaccaata aagcccggga tcatatagca gacgatcatc acctctcttc cgggggggga
    25321 tctgcggtcg aggatcagag atgaaaagag atggacgcct cctcaggata ggaggcgcat
    25381 ctcacccggg gtttatagta agatcaggag agagctcata tgggcatcga tgaaaagaac
    25441 cgctggcatt ctctcagcat tgaagaggtc ttcgagcggc tggggtcggg ggaggggggc
    25501 ctcaccaacg ccgaggcgaa gaggaggctc gaggatcacg gcccaaacga gctcgaagag
    25561 gggaaggggc agggggggct ctccctcctc atcgagcagg tcaataaccc cctcatcgcg
    25621 gttttggccc tggcggcgat catatccctc ctggcgaaca agaggatcga cgccatcgtc
    25681 atcttcgccg tcatctgcat aaacaccttc ctcggctttt acaaggagta caaggccgag
    25741 gaggcgatcc tcgccctcca gtcccaggcc tcccccgagg cgagggtcct ccgggactgc
    25801 ccccaggacg ggccttgcca agagacccgg atcaaggcga gggagctcgt ccccggcgac
    25861 gtcatcctcc tggaggcggg ggcgaaggtc cccgccgacg ccaggataat ggaggccgca
    25921 aacctcgagg cggacgagtc gatgctcacc ggggagtcgg tcccggcgag gaagacggct
    25981 gacgccctgg ccggagacct ctccatcggc gaccgggaga acgtcctctt cgccggcacc
    26041 atcataaccc aggggagggc gaaggccctc gtcttctcga cggggatggc caccgagatg
    26101 gggaagatcg ccgggatgat caccaggacc gggaagaccg ccacccccct caagggtcgg
    26161 accctggacc tgagcaagaa gctgatgctt ctagccctgg tcgccagcag catcaccctg
    26221 gtggcggggc ttctcagggg ctttgagttc atcgagctcg ccctcttcac cctggcgatg
    26281 gcggtctcag cgatccccga ggggctgccg gcggtgataa ccgtcaccct cgccgtaggc
    26341 gtcagccgga tggcgaagag gaacgccatc atccggaagc ttgacgccgt cgaggccctc
    26401 ggctctgtca ccgtcatctg caccgacaag accgggaccc tcaccaccaa ccagatgacc
    26461 gtccagaaga tcttcctggg gaaggggatg gtggacgttt cgggggttgg ctttcgcccc
    26521 gaaggcgagt ttcagatcgg aggccaacgg atcgacgccg ccagcgatcc ggacctatcc
    26581 gccttcttgc ggatagccgc cctctgcaac gactccgtcc tcaaggcctc ggaggacggg
    26641 gacagcgtgg ggtggaagat cctgggagac cccacagaag gggccctgat cgtcgcctca
    26701 gcgaaggccg gcctggaccg ggatcggctc gcagaagatc atcccaggat cgacgagatc
    26761 cccttcgact ccagcaaaaa gtacatggcc accgtccaca gaacgccgga agggggggct
    26821 gtcgccttcc tcaagggcgc ccccgaggtg atcctgagct tctcctccga gatcctcgtc
    26881 gacggccgga ccgagggtct ggaccggggc cggaaggagg atctcctcaa ggcgagcggc
    26941 gacatggccg cgggggccct gagggtcctg gccatggcct atcgagagct ggacgaggcc
    27001 gaggtcggat ccttcaagga ggcgggtccc gaagggctcg tctttgccgg cttctccggg
    27061 atgatcgatc cgccgaggcc cgaggcgata acctccatcg ccctctgcaa acgggccggg
    27121 atcaaggtga tgatggccac cggcgaccat aagatcactg ccgaggcgat cgcaaaagag
    27181 atcgggatcg cctcagaggg atcgaagatc ctcaccggct ccgacctcga agagatggac
    27241 gatgccgccc tggaggatgt gattgaggat gtcgccgtat tcgcccgggt ctcgcccgag
    27301 cacaagttca gaatagtcga ggccctgagg aagcggggac acatcgtggc tatgaccggc
    27361 gacggggcta acgacgctcc cgccctcaag tcggcggagg tggggatcgc catggggatc
    27421 gcggggaccg acgttaccaa ggagacggcg gagatgatcc tcaccgacga caacttcagg
    27481 agcatcgtca gcgccgtcga ggagggccgg gtgatctttg agaacatcag gaaggtcgtc
    27541 aagtacctcg tcagcaccaa cgccggcgag atcctgacga tcatcaccgc catcaccatc
    27601 ctgatgatca acgccctaat cttcaccccg gtccagatcc tctgggtcaa cctggtcacc
    27661 gacggcctga tggggataag ccttgccatg gagccgaagg agaagaacat catggatcag
    27721 cccccgagga ggcccggcga aaagataatc aatcgagaga tcctccagaa cgtcatcttc
    27781 gtggctatat tcatggctgc aggaaccctc ttcatattca ccagggaatg gaacggcggc
    27841 gagatcttga gggcccagac gatggggttc accaccatgg cgatgttcca ggtattcaac
    27901 gccctcaact gcaggtctag gaccaattcc atcttcagga tggggttctt ctccaacaga
    27961 tatctcatcg gagccgtcgt cttatctctg accctccaga tcctggtgac cgtcgtacct
    28021 ctcttccagg tcgccctcgg aaccatagcc ctctcccttg aagactgggc gatgatcttc
    28081 ctagtctcca gcaccgtcct cttcggagac gaattgagaa agctggtgtc caggaggagg
    28141 atccgcggtt aggcgcgtgc cagaagtaag gggggacggt tcgactcgcc gccccccgat
    28201 ccacgaattc gaggctgccg caccagaccc gaaaataggg tatgatcaaa atttgtttta
    28261 tctgatcaag gcggttcagg gggccatcta tttatccacc tcccgcataa cctccagcga
    28321 catggaggga gtttgacgga agatgagatc ttcagggacc tggggataac ccccttacgg
    28381 aggcgtgtca gcagcggtag gcccgcgccg cacctatccc tcaggttcga ctcccacctc
    28441 ttctcgatag acaccagccg ctcgcccaaa agctccgtcc agcccgacgc ctacctcata
    28501 acccacgccc acagcgacca ctacggcaag tccgccatgc tctcgcccga cgcgatcgcg
    28561 tcccgggaga cggcccgggc cctggagatc aggcacgctc gaaggtatga ggggaggacc
    28621 tttccggtcg gaggacgcat cctcgtcgac gacctggagg ttgtaaccta ctccaccggc
    28681 cacaccatcg gctccgtcgc cttcgggtgg gagacggatt tgggggtccg ggttctcgtc
    28741 accggggacg tcaagaacta cgagaacctc cccaaatgcg acctcctggt gacggaggcg
    28801 aactacggcg acccctggga ccctgcctgc cggttcgagg acgacatcgt cggcttcggc
    28861 gacgccgtcg atgccggctc caccttcgga gcctacgcct tcggcaaggc ccagagggcg
    28921 gtctccctca tccgggccct cggctgcaag gaccccatcg ggatggacgg aaagagcctc
    28981 gccctcaccc gggagctcct ccccggcttc ggccccttct cgccggcgga ggagaacggg
    29041 acagacctga acgtcgtaac cccctgggag ctctccacca tcaggtcgaa gaggaagtac
    29101 gtcctcaccg ggaggagcga cctccccttc gcccagatcc ggatcagcga tcatctcgac
    29161 ttcaaggggc tgatgaggat ggtggagcgg atctccccga aggccgccct agtttaccac
    29221 cccgagggga ggagggcgaa tatgatggcc caccacctcc gggagctggg gatggcctcg
    29281 atctccgtca gcgagatcga ggactctttt taggcagtcc cgaggcgcag gatcgcggct
    29341 ttcatcagac atctaaagat agtgccggtt catagtcagc tccttcgaga gatcacaggt
    29401 agtaaatcaa aagcggcacc atcagaaccc ccatggcgtg ggtcttcctc acccccgcga
    29461 agggggccac caggccgacg accgtcgaga cgaggtagat gaagaggccg aaggggccgg
    29521 tgaaggcgta ggtgagggcg gtgaggaggg cgaggatggc gatgcagagc ctcccgtagt
    29581 cgagccgtct cacgaccttc gccgctgccc tccccgccgc gagggtggcg aggtatgaga
    29641 ggatcgatac gaagaccgcg accgtcacca tgaagaggag gagcctgcgg tccagctcca
    29701 ccagctcctg gatggcgacg gcggccccgg accggggccg ccccaccacc aggagcgcca
    29761 ccatggtgag gagggcgttc gcggtgttca cccccgatac ggagacgaga aactcccggc
    29821 cggaccagtc cgacccgaac ctggtagcta cggtcgccac cgcgggggtc acccccggaa
    29881 cccaggccac caccgacccc gcgagccccc cgaagaaggc ggccttggtg atcgtcctcg
    29941 ccggcatccc aaaccccgtc tccctctggt cggggacctc cgcccgggat ccgaggctga
    30001 tcaggaggat cgaggcgccg aagagccctc ccagcagggg catcaggacc tccggccgga
    30061 accccagggg cgactgggcg aggttctggt gggcgaaggc gaagctcccg aggaggccgc
    30121 tcgtcaggaa gaggacgagg gcgatgagct tgtacttgag gggggcgagg gacccctgcc
    30181 cctcgatcac ctctccccgc tccgtcagga tcgtgacggc ggctatcgcc aggaggacga
    30241 ggccgacgtg gtccatgaag gtctcgtaga acctgccgaa gaggagggat agggggacga
    30301 tcagaagcag ggccaccagg atcgaggagg cgctcccgag ggccgagagg cggaccgcct
    30361 cgatcccctt cccctcgagc atcatctcgt ggccgggaag gacggccagg gcggtatcgg
    30421 cgtcgggagc accaatgaat atcgatggca ctatgtcgag gaaggtgtgg gcgacggacg
    30481 ccgccagaat cgccgtcgcg aggtcgaggg gttccagccc caggctgagg aaggcgggag
    30541 agagggccag gagaagtgct gcaaagttat ttgaatgaat gcccggggta aggccgctga
    30601 agatcccgaa tagaaggccg aaaaagaccg atgagatgag gataatatct accattttat
    30661 tagagagtag tcgctgcctg aatataaaaa agtttctgtt aagccccgaa agctggcccg
    30721 atccgcggag gggccgggcc gccggggtcg tgaaaaccct taaatactct taatcaggtg
    30781 gttgtagcga tccgcagagg gagcggaagc ccgcccctct ctggcgcagg gacccgaagg
    30841 tccgaacgcg ccccagggcg cgtgagtcgg cccccggcga gggggctagg cggaggattg
    30901 atatgaagaa ggtcaagaag acaaacccgg gattggtcac cctcatcgac gatctcaagg
    30961 ccgccacccg gaacgacggg tcggcgatct ggcgcgacgt ggccaagagg ctggagaagc
    31021 cgaggcggaa ctacgccgca gtaaacgtca gcaagataaa caggcatacc gcccccgacg
    31081 acctggtcct cgtggcgggg aaggtcctgg ggaccggcga tctcgatcac aaggtgacgg
    31141 tggccgccct ccagttcagc gaacaggccg cgtcgaagat aaagtcggcc ggtggcgaat
    31201 gtctcaggat agatgagctg gccgagaagc acccgaaggg ttccggcata attattttaa
    31261 ggtgatctga tgattattga cgcatctggg atgatcctgg gacgtctggc cagcctcgcc
    31321 gcttcagagc tcctcgcagg agaggagata gcgatcgtca acgccgagaa ggccataatc
    31381 tcaggtcggc gagagaccat ctaccgggag tacgaggaga tgaagaacaa gggctccacc
    31441 gagaaggggc ctcactatcc caagcgcccc gagaggatcc tgaagaggac ggtccgcggc
    31501 atgctccccc acaagacgaa gcgcggccgg gacgccatgt ccaggctcag gatttatgta
    31561 ggagtccctc ccgagctcaa ggggatggag atggagcggc cggaggctgc gaagatgacg
    31621 cgcctgggca ccgggaagta tgtggaactt ggcgacgtaa gccggatgtt cggatcaaaa
    31681 ttctgagggt tcagcatgaa gataaccaac tcatcgggta aaaacagaac ggcgatcgcc
    31741 agggccaccc tgacggaggg gaagggcaga gtcaggatca acagcaaacc cctggagatc
    31801 tttgagcccg agctcgtcag gcagaagatc atggagcccg tgatgatcgc ccaggagaag
    31861 gcctcggggc tcgacatcga cgtcaaggtc cgcggaggcg ggttcatggg tcaggctgct
    31921 gccgtccgga ccgccatcgc ccgcgggatc gtggagtgga ccggagacac cgccctcaag
    31981 gaggcctaca tggagtacga caggtctctt ctggtcaacg accaccggca gaaggagcgg
    32041 aagaaggtcg gcggccccgg tgcaagagcc aagtatcaga agtcttacag gtgagctagc
    32101 ttgatccctg tacgatgttt cacctgtgga aacgtcatct ctgatgtatg ggaggagtac
    32161 aaggagcgca ccaagtctga gccaccgggg aaggtgatgg acgatctggg catcgaaagc
    32221 tactgctgtc gtcgcatgct cctgacccat gtggagatcg ttgacgtgct tcggcggtat
    32281 cagtaaaagg cgtggggttg tggggtagcc tggtccatcc ctcggcgttc gggacgccgt
    32341 aacctgagtt cgaatctcag caaccccacc acttgccttt cttttgaggt ggccacttga
    32401 cggattcgat ggatgaagaa tatacgcgat acgaacgcgc gagaattgtg ggggctcgag
    32461 ctttacagat cctgatgggc gccccggtat tgatcaagac cgagtccata gaccccctcg
    32521 aaatcgccct ggaagagatg agattgggat ttgttcccat caccgttaaa cgggatcgca
    32581 gaacctctag gtaggtgaca tttttggaag atgaagtcat aacaccagct gaagattatg
    32641 agaccctcat ccccatagac gagtatctag ccgcggggat ccacataggg actcagcaga
    32701 agaccaagag catgatggac tacatataca gagtcaggac cgacggcctc tacgtcctcg
    32761 acgtccaggc gacggacaag aggatcaggc tcgcggccaa gttcctggcc aagtacgacc
    32821 cc
//
LOCUS       DASM01000038            2893 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_195001, whole genome shotgun sequence.
ACCESSION   DASM01000038 DASM01000000
VERSION     DASM01000038.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_195001"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          520 a          908 c          906 g          559 t
ORIGIN      
        1 gtcgccgacg acgaggtcga tcccgcgtat cttcgccgca tttatatctc ttgaatgcct
       61 tattttccag gtcggtgaag agaatgagcg agacggttga ggtgtccagg gaggtcttcg
      121 agccgatctc catgctggtt cgagacggcc tattcaagga cgagaaagac gccctgagaa
      181 gcctcgttct ggatcaggcc gcatcgaaga tccgccactt cgactccctg atcgaagaga
      241 tgcgcgccaa gtacgatatg agctttgagg agttcgaaga gcggatcaga tcgcggaagg
      301 gcgctgagtc cttcgacgag tgggacgact tcatcatctg ggagagctac gagtcggccc
      361 gcaaatactg ggcgggcgtt caggccaatc tcaaaggcat cggagaatga tagtccagga
      421 tatcctcgac agcctctcat ccagcgagat cgtcgaggag attgaagtaa tggtattggt
      481 ccaggagccc ggaagacagg cgctccgggc cgttgcatcg ctgaagggag gatacttcct
      541 ctccatcaac gaagcattgg gcagggactt caggcgttat tcctaccacg tacaaaaaga
      601 ggagaagatg gtccgaaggt gggacaacgc cccccactgg ccggatatga agaccttccc
      661 gcatcatctt catatcgaga gcaataagaa cgtctcggag tgccaggaag ttctcattgg
      721 ggacgtgctc aacgagatgg aggccatcat caaacttgag agcgctgttc cctcttgact
      781 tgtgaaggga gtcacctgta cagacctggc agttaattaa aatctctttt taccagtcac
      841 ggttggcgga aaaccgctgc aaagttccag ctcccccttc cgaaactcgc agaccccctc
      901 caccgtcacc ccctccggca gagtcgcgaa cctcgccctc aagatcagat ccgccagctc
      961 ctcgcgcatc actttggcga tccccacgat ggaggtggtg ggcctcgggt tcacgtccac
     1021 gacccggggg aggtcgccga cgacgaggtc gatcccggcg tagcccgaaa ggtcgagggc
     1081 gcgggccgcc atcttcgccg ccttcaggat ctcctcctcc cggtccgagc ggtaggggac
     1141 gatcccgccc tggtactcga agcccgcctc gacgctgacg agctgcttgt tgacggtcag
     1201 cgggaggacc cggtcaccgg aggcgatgag gctgacgctg agggcctcgc cctggtggaa
     1261 cctggtcgcg attctgtcgc ccaggtcttc cgggagactg tcgacgagct ccaacctctc
     1321 cgagccgcag ccgaacctcg gcttcaggac gaaggggccg gttccggggt cgtcgggatc
     1381 ggcgatctcg acgacgggaa cgcccgcctc ttccaggcgg cgggtgcagg cgagcttatc
     1441 ggcggagagc ctcgcggcgg cgggggagca gccgaggttt gcggtccctt tttccaggat
     1501 ctccagaaat ccgggcagaa gctcgtcggg agagattaca agccctgcgt ccgtgcctga
     1561 accggcgagg aactcctcga agtcccgatc gcttttgccg ccgaggacga ttccccgccc
     1621 ggcgccgatg gtgggccccg ccgtcgggta gatgacctcg tggccgagcc gctcgaagct
     1681 cctcgcgagg atggagagca tcgccctccc ctcgacggcg taggtccccg gcagatcagc
     1741 agccacggcg tactcggcga tcagaatctt catggaatct cggcgctgcc ttagtactgc
     1801 ctcgcgagaa gggcgcactt ctcggtcctg gcgccgcaga tgatgcagct tccctggtag
     1861 gtttcttcag tccgccaggg ctcgccgagg acggcggcgc cgacctcctc ctcaatcctg
     1921 tgaccgcact cctccgagcc gcaccatccg acccgggcga ccccggccgc ggtctggacc
     1981 ctcgcctcct ctgccgtctc gcagtccttc accttgctct cctggaaggc ctgggccttc
     2041 gccagcatcg cctcgtgaag atcggcggct tcattcagga ttgtctgctc taagtcgact
     2101 aggggaacgg tcagcttctt gccgtcgcgc cttgccagga ccgcgacccc gttttcgatg
     2161 tcccgtggcc cgatctcgat ccggatgggg acccccttca tctcccactt gtagaacttc
     2221 gcccccggcc gctcgtcgct ctcgtcgatg gccgcccgga tcccggcagc gtctagcctc
     2281 gctttgatct cccgagagac ctccagggtc ctctccttat ccttgaagag gatcgggacg
     2341 atgaccacct ggacgggggc gaccgaccag gggaggacca ggcctttgtc gtcgccgtgg
     2401 acggagatga gggcggcgat gcacctctcg gagacgccgt agcaggtctg gtttgcaaac
     2461 tcctgctcgc cgccctcgtt ctcgaaggtg atcccgtagg tggtggcgaa gttcgtcccc
     2521 aagaggtggg cggtcccgat ctggagcgtc ctcccgtcgg gcatcaccgt gtccagggcg
     2581 atggagtagt ccgaccccgg gaacttgtcc caggagggcc tcctcgcgaa gaggaagggg
     2641 atggccaggc gccggtaaaa ctcggcgtag atggtgaggg ccgcctccac ctgctccgcc
     2701 gcctcatccc aggtcgcgtg gacggtgtgg gcctccttga aggaggtgat ctcccggagc
     2761 ctgatgaggg gccgggtgtg cttcgtctcg taccggaagg tgttgacgat ctggtatatc
     2821 ttgaggggga ggtcggcgtg gctcctcacc cagagcttga agatggggta tatcgccgtc
     2881 tcgctcgtcg gcc
//
LOCUS       DASM01000039            7427 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_23093, whole genome shotgun sequence.
ACCESSION   DASM01000039 DASM01000000
VERSION     DASM01000039.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_23093"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1257 a         2380 c         2420 g         1368 t
ORIGIN      
        1 cgccgctcgg cggctcccat aggggctgaa accagggctc ataagctccc ccggtcagct
       61 ctgcgatgac accttccgcc tcgccgtcgg ccccgcccca ctcggctccg gtgttggagg
      121 cggatacgta gaggaagagg gcaaagaaga ggatgacaac aaagcccgct atcatctcac
      181 ccctcatgcc gggatcgccc ccttcagctc tcccagcctc ttctcgtcga tgaccctcag
      241 cctcaccatg acgtcgcctt tcacctggat tatgtacttg aagatcaggg ctatcaccac
      301 cccctcgagg atggcgagag ggatctgagt gacggcgaag atcgccccga aggcgtagaa
      361 ggaccccacc acccctccgg tcgccgaggg gaaggcgagg gcgagctgga gggaggtgac
      421 gatgtaagtg gccaggtccg ccacggtgac cgcaaggaag atcgagatgt agaagttcag
      481 cttcagcttc ccggcggatc tgtagacgag ataggccacg gcgggcccag cgatccccat
      541 ggagaagagg ttagccccca gggtggtgag cccgccgtgg gcgaggaaga gggcctggta
      601 gacgaggacc accaggcata ggactgcggt gatcgccggg ccgaagagga tcgcccccat
      661 cccggccccg gtggggtggg agcaggagcc ggtcactgag gggagcttga gggaggagag
      721 aacgaagatg aaggccccgg cgagggccag gagggggagg gtctccctcc tctccttgac
      781 cagctggttc atcctgtaaa ttccgtagac tacgacgggg agggagaggg cgtaccnncc
      841 tcccaccagg gatgggggag gaaaccttcc atgatgtgca aaatatcact tcctcttcga
      901 tgatcgggtc ttcctcctgg aggtgggcat catggcatat aaagtttgct tgagatggga
      961 tcaattaact ttatttgcgg ccgtaaaggt cgacggcgac cctcccggag agctctaccc
     1021 acccatctcg gtaccgggcg gcgagggcca tcgcctcgac gcccccggcc gccgcctccc
     1081 ggagggcggc cccgaaggcg gggtcggtct cgtcgttggg cctcaaggcc ggagcgccgc
     1141 ccctcatgac gacgaagacg atggcggccc ggcagccctc ccggaccgct cctgccagct
     1201 cctgaaggtg gcggcgccct cgctccgtgg gggcgtcggg gaagagggcg accccatccc
     1261 ggacgagggt gcagctcttc gcctccagga ggcagggcct ctcgcctgcg aggaggaagt
     1321 cgagcctgct cttcccccgg gccggctcgg acctcaccag ctcatacccc gcaaactccg
     1381 ggagctcccc ggaggagagg gcgaccccca ggatctggtt tgcaaccctc gagtcgacga
     1441 ctacggtctc cccatcggcg acggcggcga agacgtcgaa cttcgtcttc cgccccggat
     1501 cttttgccgg ccggaggagg atctccgccc ccgggacgag gagctccccg agcctgccgg
     1561 ggtttgggag gtggcagctc tgctcctctc catccaccgg accctctgcc acctccgccg
     1621 tcaccgagaa ccggctcgtc ctggagagga acctcccccg gaccatctcc ccctcgatct
     1681 tcattcgggc catctctttc gctctgcgcc gcgagccctt aaatatggta gagctcccct
     1741 attcccccat tgaaccgagc attcaccgtc accgagctga accagaggat ccgggaccgg
     1801 ctgaccggcg acctgaggct atgtgatctc tgggcccggg gggagctctc caacgtcgtc
     1861 aaccaccgct ccggccaccg atacttcacc ctcaaggacg gagagtctca gatcccttgc
     1921 gtcctcttcc gggggaacgc ctcccgcctc gacttcgacc tcgccgacgg ccagaacgtc
     1981 ctcgtctttg gggacgtgga gttctaccgc ccccagggaa gggtccagat cctggtgaag
     2041 gcggcgaggc tcgacgtcgg cctcggcgag aagcaccggg agctggagcg tttgaaggaa
     2101 cggctagccg ccgaggggct cttcgacgcc gaaaggaaga ggcccatccc cgcccatccc
     2161 gcttgcatcg gggtcgtcac ctcccccgac ggagccgccc tgagggacgt cctgagggtc
     2221 ctcggcgcct gccccgcccg gatcgtcata tccccggcgg tggtccatgg ggactctgcc
     2281 cccgcgtcga tagcctcggc gatatccgcc ctccagggga tcgccgacgt catcatcgtc
     2341 ggcaggggcg ggggctcggc ggaggacctc tcccccttca actcggaggt ggtggcgagg
     2401 gcgatctatc agtcggcccc tccggtcgtc tccgccgtcg gccacgagac ggacgtgacg
     2461 gtggccgact tcgtcgcgga cctccgggcc cccaccccct cggcggcggc ggagatggtg
     2521 aggccggacc tcgagaggct ccgggaggct ctggaggggg cggaggcgag gatggtccgg
     2581 tccctggcgg cgagcctgga gcggaggagg gcgaggtacc tcgccctagg ccgcctcctc
     2641 gccggtcgca ggatgatggc gatagtctcc gaggagcggc agagcctcga ccggatgacc
     2701 gaccggctcg tctcggcgga ggatcggcgg atggagctct tcgccggaag gctggaggtg
     2761 gccgcgggaa agctcgactc cctctccccc ctatcgactc tccggagggg ctactctatc
     2821 gccctcgccg attcaggggt cataaggcgc gcctcggatc tcgccccggg cgagctcttc
     2881 gagctcgtct tcgccgaggg gaggctcctc tgccggtcgg tgaaggagat ggcggatagg
     2941 agttgatggg tttgaaggat gcggtatcga ccgatttgaa cgccccggag gatctcagcc
     3001 tcgaggcgtc tctggcggag ctggagggga tcgtccggtc cctggaggag ggggagctga
     3061 ccctggagga gagcctgaag gccttcgagc ggggggtctc cctcatcagg aggtgtaact
     3121 ccctcctgga cggggcggag cagaggatag agatcctcgt cggggagctc cccgaggacc
     3181 tggcgaggtg acccgccatg ggtgatgccg aactttgtcg gtccttcact cggcggacga
     3241 gagccctcct ggaggcgggg gaggaggcga ggatcgtcct cttcggcgac tccatcaccg
     3301 ccggatttgc cgtccggagg ggcttcgacc atttctggct atcgatgctg aaggagaagt
     3361 acccgagggc ctggatcgcc atgcagaacc agggggcgtg cggagccacg agcttcgacg
     3421 gcctcgcccg cctcgactgg tcggtcgtcg ctcacaaccc ggacctcgtc accgtcaact
     3481 tcgggatcaa cgacatgtat atggggatcc ggctcggcga gttcaagggc aacctcatcg
     3541 agatcgtcga gaggatccag aagggtccag gctccggatc gggccccgag ctcctcctcc
     3601 tcagctccga gcccctgatg acgccaaagt tcgatcgaat cgtcctcggc tactatcagg
     3661 tcttggagga cgtcgccgag gagatggggg tcggcttcgt cgacgtttac ggggcgtgga
     3721 tgcggcgggt cggcgagggc gtccctctgg acagcctgat cctcccgggg ctcgaccacc
     3781 cgaatgagct ggggtacaga atcatcgccg aggagctgat gcgcttcttc tagctccacc
     3841 cgcttccaat cccggcccta ccctttcggc cctccatcat tttgcctccc tctctaccgt
     3901 tttccttcca tctcgtcgac gaaggcgttc cacctcttga cgtcgttcac cttcttctta
     3961 atcacctctc ttctgacgtc ggtcttgtgc tccttctttg ccaggatctc ccgcaggtcc
     4021 aaaagggacc tgatgacggc agccgtctcc tcgtagatcc ggtgcgcctc ctcccgggtc
     4081 agtcgcgcct ccttcagctt tccctcgtcg gccgcctcct tcgccttcag gacgcggatc
     4141 agctccctca cctccatctc ctccttctcc gatagctccg agaggtggat cagcctccat
     4201 acgaggtggc ggaggtcgac ggtccccgtg gcggggtgga cctcgggcgg gaggtcggcg
     4261 ggggcgatcc ggtctcttgc catctcctcc ttcaggaggt cggggtcgat ctcgagcctc
     4321 tcggggtcct ggacccccag ccagaccagg gtccggtgga ggttggcgag gagccggtct
     4381 ctctcccggt cggtgaggag gtcggcgggg gcgggggaat cgggcggcgg ggaatttggg
     4441 ggcatttgat atagtctttt attttggcgc tagttaaagg atgctatgtg ccccaaccag
     4501 ctcccatgtc cacttagtct gcacctacaa ctcggcccgc ccccaatcag ccgagaggct
     4561 tctagcgcca cctgcacggc gatcgctatg aggtcgaaag cgccggaacc gacccgatcg
     4621 aggttaaccc gctcgccatc agggcgatgg cggagcgggg cgaggacatc tcgggccaga
     4681 ggtccaagag cctgaatgag tttcaaggcc aggagttcga cctgatggtg acggtctgcg
     4741 atcgggccag ggaagcctgc cccttcttcc ccggcggcgg gaagagggtc cacaaaagct
     4801 ttcccgatcc cgcagccgcc gccgggaccg aggaggagca gcttctggcc ttcagggaag
     4861 agagggatat gatcgaggct gttgatgtgc aataccctga cctcccagtt ctatgcggct
     4921 tctggccttc agggaagaga gggatatgat cgaggcctgg atcgaggaga acctgggcta
     4981 aagagcgggg tttgcgatca actttgaata tatgaatatt ctagagcaga tcatccagct
     5041 cttctctgca gatgccagcc tgttttagaa tggggagggc ggtccccttt gggagatcct
     5101 ttttgtggaa aggaaccaca actctgcact tcgcatcggg attataataa atgcggtggc
     5161 tgccgcgtat cctgtccagg acgaaaccct tcttctccag caccttcgct atcttctgag
     5221 gcgtgagggc tggcacctta ggcatgggcc tctaccgata ggatgcgctc cagagtctcc
     5281 tcttcggtcg ggatctcttc accgtgctct ctcaggctct caaggtagag ctcgatcgcc
     5341 tctcttgcca tctccaccgc ctcctcgatg gtgtcgccgt aggtgatgca cccggaaaga
     5401 gatggaacgg tgacggtgta gccgccttcc ggctcctttt taagcaggat cctgtaggtc
     5461 agtgtgccca tgaagattac tggtcgttta tagtattaga agcccaccag gcatcaagag
     5521 cagaaaggca ggatcggggc cggagggggg cggggccgtg gaaaaaatgc ccgctacccc
     5581 ggcctcacgt accggacccc ctccggcgcc gcctcattga cgaggacgac gatcgtctcc
     5641 gagaggcggt tgaagagccg ctgccccgac cccggcatcg cgatccagcc gatgagaacg
     5701 tccacgggga ggaggaaggc cttcccgaag gactggatgg cggcccgggg gaggtcgatc
     5761 gcatcgcccc gccggtcggt gaccttgagg ccgagggcca tctttcccag ggattggccc
     5821 cgggtcccct ccaggaccgt ccagtaggcg aataggatga gggcgtcgcc ggagccgagg
     5881 tggacgaacc aggcggagaa gatcggcccc ggcgggagcc agaagtggaa ctgggtgatg
     5941 ggtccggccg cccccgccag gaggtcccag aggatcccca ccaggattat gtcgatcagc
     6001 caggctccga ccctccgccg ccaggaggcg atccgaacct cccgaacctc gccatctctg
     6061 ccgttcatcc gatcaccagc cagaccgtat caggaagagt tatttttttt gggtgtggtg
     6121 gcctcgcgcc ggccgccgcc ggaaggggcg gcgccgcccg ccacctttcg cccctcagta
     6181 gacccgctcg ttgtgcatcc tcgtgctgta gagccggtat tcatcccggt tcttatcctg
     6241 gaagaagatg tagttgtacc gggagtcggt cttgtgggcc tcgtactcct ccctcgcctc
     6301 ctcgcggcag ctcttgcagg cgaatagcgg cacgaatacc ccaaagccgc tctccgactg
     6361 gccgtatccc ctgacggcat cggtgtcgat ggtcatgtag ccggggcagc tcctgtcggg
     6421 gcacatcttc accgactcgg tctgcctctc ccggatcccg tcggtgtcgt agaagatgtt
     6481 cttatgcctc cggaagaagg gccccgccct ctcgacggcc ttcgccgtca gctcctccct
     6541 tgcatcccag accccctgga ggtactcgac ggtcccggtg atgttcgcct ccatggacct
     6601 cgacatcgag aaccttccgg ggtggtagtc cggctcccgg acgttggagt agtcgaggcc
     6661 ggccatggcg aggatgatcc cgcagttgac gtaagggagg gcggactcga tggcgtaccc
     6721 cccctccagg acggctatgt ccggcgccag catctcgttg agccgcgcat acccctgggc
     6781 cgaaaagcgc atattcgcca gggggtcggt gtagtggttg tcctggcctg cggagttgac
     6841 cacgagctgg ggccggaact cctccaggat cgggaggacc agctcctcca ggacgtaatg
     6901 gatcccctcg tcgggggtct cgggcggcag gggtatgttg atcgtcctac cgatcgcctt
     6961 ggggccgccc tgctcgtcga cgaaccccga ccccggatag agggtccttc cgtcctggtg
     7021 gaaggagatg aagaggacgt cggggtcgtg gtagaagatc tcctgggtcc cgtcgccgtg
     7081 gtggacgtcg gtgtcgatga tcgccaccct ccggatcccg tacttccggc ggatgtactc
     7141 caccatgatc gcctcgttgt tgacgttgca gaagccccgg ttcccgtggg cgaccctcat
     7201 ggagtggtgg cccgggggcc tcaccagggc gaaggcgttg ttgatctcgc ccttcatgta
     7261 ggcgtcggcg aggacgaggg tggacccggc ggcgatgagg tgggcctcgg tgacgagggc
     7321 ctcgacggag gggatgcaga agtggaccct ggcgatgtcc ctgtgctccg cgaggcgcgg
     7381 cttgtactcc tccacctccg ggaggtccat cagcccctcc tcgaaga
//
LOCUS       DASM01000040            6926 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_170842, whole genome shotgun sequence.
ACCESSION   DASM01000040 DASM01000000
VERSION     DASM01000040.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_170842"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1216 a         2102 c         2333 g         1275 t
ORIGIN      
        1 gtacatgctt gctggtttac gtgccaaaac tgaactcctc caaagtgatc ttgagcctgc
       61 ctagtagatc gatatggttt ataagcgttg ggggcggagg gcgggggggg tttagcggcc
      121 gcccccggcc tcagaccgtc gtcggggtcg acgcggagat ctctcccgcg agggagatct
      181 tcatcaccgc ctccgcctcg tcgacggagc cggtgttggc gagggcccac atcagcttca
      241 ccagggccac ctcggggagg atgtccccgc cttcgatacc tccggcggcg aggatgtctc
      301 ggcccgtgtc gtaaacccgg tcacagaccc tcccccggag gcactgggag gtgatcacca
      361 cggggacccc cgcatcggtg gcggccttta tcgaccccac ccagtcggag gcgacatggc
      421 cgagccccgt cccctccagg acgatccccc ggtacccggc ggcgatgcaa tcctcgaaga
      481 ggtcggggct ccctccgggg aagtacttga cgagggcgca cctcggctcg agccgatcgt
      541 tcacctccag ctccacctcg cccctcctcc ggaagtcccc caggatctcg agatcccggg
      601 acggataaag gaccctggcg atggggtgct ggtttatgct ccggaaggcg tccctccggg
      661 aggtgtgcat cttccggacc cgagtacccc ggtggacgag gcagaagtcg tcgctggtgg
      721 acccgtgcat caccaccgtc acctcggcga tatccgtcgt cgccaccctg gccgccgaga
      781 tggcgttcat ggcggcatcg ctgctgggcc ggtcggagga cctctgggag ccgacgagga
      841 cgatcggaac cggggtcttc accatgaagg agagggcggc ggcggtgtag gtcatggtgt
      901 cggtcccgtg ggtgatgatg acgcctgctg cgcccttctc tatctccgat ccgacggccc
      961 tcgccaggac ctgccagtac tcggccttca tgttctcgct gaggatgttg tagatcacct
     1021 tcccctcgta gttcgcgatc tccaagagct ccgggatcga tgatatgatc tcctcggcgg
     1081 agaactggct ggtgacagcc cccgtcctgt agtcgacttt gctggcgatc gtcccgcccg
     1141 tggagaggat cgagaccttc gggagcccct ccgagcgcgg caggggcgga ggcgggggga
     1201 ccttcagggc cttcccccga gccagaagct ccaccacgga ccgttccgga tcgaagccgg
     1261 cgttgtagcc gctctccatc ttgatgacca ctctgccgct gacagagggc atcaggaccc
     1321 cctctcggcg gacgccatcc tttgtgaccg ctattctatc ccccagttcc agcatcaccg
     1381 catcagctcc tgcctccttt tcgacagagg gtgatttcga gggatgaaaa acgttcccct
     1441 ccggggggcg gccatcgacg gggaggcgcc atctcttccg ccaaggtgcg acagatttat
     1501 atcctggtga taccaccctc agtgctgtat ggcaaagttg ctataagaaa acaatgttgc
     1561 ccaaaattct ggaggggggc tgaagatggt ctcatggtgg tggtcgggtt ctgaagggcg
     1621 gaccgaaggc tcatatccgg tccttcttgg caacaaaggc ggcgatcatc cccgttctcc
     1681 ttggcggtcc gggctctcgc tccccgggcg gatccccgtt cgcgcttctc ggcagggatc
     1741 tataattttc cttggcctga tcgtcctcgt ctccgcggtg gggatagtcc ccgggggggc
     1801 cctggccgcc ctggacctct ccctgacatc ctcggcaagg ggcgaagccg tcttcgcggg
     1861 agagagcttc agctacacct tcgaggtgag gaactccggc cccgccgcct ccgtcatcat
     1921 caccaccaac ttctcgatcc cggagggggt caccctggag tccttcaccg tcagccgggg
     1981 agagtacgac cggcggggtg gggtctggac cgtgggggac ctctccgccg gggaggtcga
     2041 gacgctgacc atggatctgg tggtcgcccc ttcggtcccg gtgggaacgg tgatctccgc
     2101 cgccgccagg gtggcggaag atcgggctgg cccccgtccg gaggagatcg aggccttcac
     2161 ccaaaccacg gtaatggccg aggcggaggt gagggtggtg gtgacgtgtc cttccccgga
     2221 ggagggtgga gaagatatgc ccctttacgt cgtcaatgtc gccaaccatg gccctagcta
     2281 cgcccgaaat gtctggttca tggatctcat ccccgtggtg atgtggctca acggcgccag
     2341 gtacaggaat gaggcgacgg gggatgaagg ggattggata ggttttctgg atctgggcga
     2401 cctcgcgccg ggggagacga agcgggtcag agtctacggg gacctctcac ccaccacagc
     2461 aagatcgaat ccaggggtga cgatcaccgt caggtggatg gactccgtcg atccggcatg
     2521 caactcagag gtctcgagct gccgggtcga tcggatcggt tgagtcggcc gccccaggcc
     2581 tcggtctact cgaggaaagc cgattaatat gcgtgcgatg aaggtttctg gggaggcctt
     2641 tcactttgat atatttcgtc accgggaacc ggggaaagta tgaggaggcc cgagagcttc
     2701 tgggggaggt agagcagagg aagatcgggt accccgagat ccaggccgac acccttgagg
     2761 aggtggcggt ctatggcatc cgggatgtgg tggataggct gccggggccc gtgatgatcg
     2821 aggacgcggg gctcttcatc gactccctgc ggggctttcc cggggtctac tccgcctacg
     2881 tcttcgatac catcggaaac gaggggatcc tcaggctgat ggaggagaga ccccatagga
     2941 gagcggtctt ccggtcggtg gtggcgtacg cggagccggg gatggaaccg atcctcttct
     3001 cgggggagct ggaaggggag atcgccctcg aaccccgggg ctccggagga ttcgggtacg
     3061 acccgatctt ccgggtgggg gagaagacga tcgccgagat ggacctcgct gagaagaacc
     3121 ggatctctca ccggggcgga tctatccgcg ccctgaggga gtggcttggg gcccggtgag
     3181 cccccgggtc agcatctctt cttcttctga aggacgcact tggggagcat cctgctgtcg
     3241 atctccttta tcctcggggc ctggacggcg gagatgtaga gggtcctccc ttccacctcg
     3301 agccttctgt ccatcggctt tggggggctg agcctcaccg gtaggaggat cggaccctcg
     3361 caggtggtgg ctatccgaag ctctgagaag ctctttttga tgtactcccg gatctccggg
     3421 gatatgatgg ggtcggggag agggcctttt cgggggagtt ttcctggcat tgccggattc
     3481 ttcgtcctcc ccctagaaat agtatatccg cccgatggtg ggccctcgcc ccagggagag
     3541 gcggatctcg ccctccctga ggtgggatgc cctttataag ccccggggct taacctctcc
     3601 tcatggcgag gaggaagaaa ggatactacc gggggaggga cctggcgagg cagaggatcg
     3661 agcggctctt cgtcctcgcc gaggaggagc acaagcagca tccggagagg agcgacaggt
     3721 acgtcgagat cgccaggagg atggggatga ggatgagggt ccggatcccc cgccggctga
     3781 agaggcggat ctgcaagggg tgtcggtgct acctctcccc cgggaggacc cgggtgaggc
     3841 tgagggatgg gatggtcacc gtcacctgcc ttgagtgcgg agaggagatg aggtatccgt
     3901 accggaggcc ccggtgatcc gggggctcct ccacctccca tccctggagg ttttgggtct
     3961 gggcggggtc gggggccctt ccaccatcgc gcccggcggc cgggaggggc ggtcggattc
     4021 ctccggaaga ttttcctctt caggaaccgc tcctacaccg acgcgtcggg ggcggagatc
     4081 tttagacctc gacgatcgaa gagccaggta ccccggccgc ctcgaccgga ggtttctcca
     4141 aaaattttta atataatgat cgaatacttg atcagtgtcg acgattccgg aggtggatta
     4201 tggcatacct ttggtccatt ccctggacct tcatgtcctc tctcggggtg ctggaccagc
     4261 ccggttactg gccccccgaa tacgtggcgg catcgagggc cttcctcatc ctcctgacag
     4321 cctatctggg cttcaaggcg ctctatctcc ccttcagctt gaacgatggg gagtaggcag
     4381 cccgcccccg tccctgccgc agaggcccag agcctaggtg gcccactttt ccgaggctgg
     4441 gcctgcagga agctatttct aaaatgttct actatgtaca ataccatgca gcttcgctgc
     4501 ggcaggtgcg gcgctcgctg ggagtacagg gggcgggcgg aggtctacgc ctgctgcccc
     4561 cagtgccggt cgaaggtgaa gatcgacctg gaggggatcc ccctccgcct ggagcggtgg
     4621 gcggtccggg tccccctgga ggtccgggtc cccgacgacc tcccgggggc ggcggaggcg
     4681 ttctaccggg ggaggctgga gccgcccttc gggccgatag ccatctccac ggccatcagg
     4741 cacgaccaca gcaactacga cgagctggtc attcggctgg agaagaaggc ggcggaggag
     4801 ggggccgatc ccaaagaggt gatggccgcc tatcagatcc tgagggagag gttcgaccgg
     4861 gagatatgca cccgcctctg gaggtcgatg gcctgcaggt gcgacaggag ccatctggac
     4921 ggcggggctg aaacccccaa tactcccccc cgggagcggc ggaaggccgg cggagctgac
     4981 ccctgtagga gaagatcgat atgaaggaag ataagccgag gccttatcgg ttcgccgagg
     5041 ccctggggcc tgaggctcta ggggcctttg aggcgctatc tgccgaggtt cttaaggacg
     5101 gcgccataaa ggggaaggag aaggctctga tcgccctcgc ctctgccgtc gccgtcaact
     5161 gccggtactg cgtcggggcg cagaagcgac acgcatcgat ggccggagcc agccccgagg
     5221 agatcctgga ggcggcggcc gtcggcgccc tcgtccggct agggtcgggg ttcacctacg
     5281 cctcctacct cctcgacgat ggagaggacg aggctcctct ccccagagga ggcgattgag
     5341 atggagggcg acggccgctg tcagggctgc ggccaggagg tgggggagga ggagctgatc
     5401 ctccacgagg gggagaggct ctgcgaggag tgctacttcg accggtgcca ccggatcaag
     5461 gtctgcgacc cctggggcga gaggtccaag cgggtattcc gggagatggc ggggctggag
     5521 gggacggagg ggctgacgga ggtccaggcg aagatctacg agttcatcag gtctagggac
     5581 ggggcatccc ccgaggagat cggccgcgag ttcggcctca gccccgcgga ggtgcagaac
     5641 gagtttgcga tcctgcgcca ctgccagctc atcaagggga agagggctga aggcaagatg
     5701 ctcttcgtcc cctggtaggc gctaaatcag aacgaggccc tgggggcctc cccctccctc
     5761 atgttgaact ccatcgtcca gaatcgggcc tctccgtggt gcatccggaa tacggccagg
     5821 ttcgggtcct gggggccctg gacgatcgcc ttcaggaagg gcctctcctc gatcagccgg
     5881 gccctcagcc cggcgtcgtc gagaaactcc acctcccccg cgaccctcat aatcttatcg
     5941 tggctttctg aagagaagca gacctcgacg agagggtttt tccggagctg ctggcagacc
     6001 ttaacggtcc ggtccgggta caggaggcag agggacctga tggtgaagtt gatcgaggag
     6061 agcttccccg ccggggtcga cggcaccagg ctcgagacgg ggatgttcct ctgggtgacc
     6121 ctccccgaga atatgtcgtc attggagctc ttcgaccgcg ccatcaaaaa cgacgtcgac
     6181 ttcgttccgg ggcaggcctt ctacgccaac ggcggcggct acaacaccct gaggctgaac
     6241 ttctccaact ccgacgagga gaggatcgtg gaggggatgg gaaggctccc gaggtccatc
     6301 gaggagatga tgtagccgcc gaggtgggga ttggatggcg gagcttctcc ggcggcggcc
     6361 cattctgaga tcctcgcccc atcgaggctc tccccctcga acggagattc agtccctcga
     6421 atccccgaac gccagcctcc gggcgtacgc ccgccgttcc cgaccgcccc caccgccaga
     6481 gattaaatcg ttcagtttat ataaattcac cgggcggagg accacccggg ccctcccgcc
     6541 gccgccgacc ggatccctca cctcgacctc ggcctcctcc gcctcgggag cggtcgtagg
     6601 atagaggatc gccgacctcc ctccagcgcc ccgccctccg atgagggcgt agatggcgag
     6661 ctgataaagc caattccggg ggagggagtt cgcccatagg tcccgatact tggcgtcgag
     6721 gatggagacg acctccggcc ccctcaggat cgcatagtcc gggcgggggt ctggagcccg
     6781 ccgattcctc gggttctttc cggggacgta ggagatcatc ccgcggaggc ggcattcgtc
     6841 cacgatagtg tagcccggca ggttctcccg gagaaaccgg gacagaagcg cctggaagaa
     6901 ccggttcatg tcgaagagga agccgg
//
LOCUS       DASM01000041            7561 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_57356, whole genome shotgun sequence.
ACCESSION   DASM01000041 DASM01000000
VERSION     DASM01000041.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_57356"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1284 a         2527 c         2484 g         1264 t
ORIGIN      
        1 gcggccggat cgtcgccgag gggacccccg aggaggtggc ggccgtcccg gagagcatca
       61 ccggcaggtt cctgaaggag aaggtctccc gccggggcgg cccctccgga gcggaggctg
      121 gaaggtcgga gacggtctga tctttcccgc agaaccgaag ccttagatca gctcttcgcc
      181 atctttggcg gttcaagaac cccgtcagaa ccagacggcg atcgccctga tctccgagac
      241 ccgccactgg ccggggccga aggtcccccc ctggctcctc ctgaagacgt ccttcgtctg
      301 gggatcgatg agggtcatct cgatcccgct gtggagggga tcgccgtcct tccggcaccc
      361 tccggcctcg ttcccggtgc tcgggcccgt ccggctggag aagccctcct cggcgtactc
      421 cgccaggtcg tcgctgatga cgcagtcgtc gaggcctgcg gggtccttcg gccccggatc
      481 ggcgaagagc tcgtcgaagg tgacgacccc caccacgtcc cggggctggg ggctttttag
      541 ccgcaccatc ccgcaggcgg accggctctc catctccttg aaggcgtacc agagccgccg
      601 ggcctggtga tccgtctccc cccgatctcc gttcaccgat ccgggaccga aggactggac
      661 gagctcccag acctccgaga gggagagggc caggtagcag cgatcgctcg ggtagatcga
      721 ctcgaagggg gcgctggtgg cggcgtggac cgattccaga agctctcggg gggataggac
      781 cctcttgggc atggccggag gttaatcaat ccgggtataa aatattttgg tggggatcac
      841 tcctcgaagg tggagaactg gcgggagagg tgggagacca tcgcctccac cgaccggcgg
      901 agtacggccc tgttgtcgta gctggcgacg atcttccgaa gctcggcctc cctctgcccc
      961 cccatctcct ccaccaggga gatcaggttc gggagagccc ggacgacgtt gtcgacgatc
     1021 gtcaccgtcg aggtcctggc ggtccgagag agggggttca gatcgacggt gacgacccgc
     1081 tttcccatcc tcaccagggc ctcgcaccgg tctccgtcct ccagggggac gaagacgacg
     1141 tcagcgtcga agatcccgcc cctagtcgcc ttcccccggg cgtggtcgag gccgggtacc
     1201 gaggcgtcgg gggtctcgcc gaagacctcc tcggcgccga aggaccggag gtggtcggct
     1261 atcctccgga cccgctcctc ggtcctgtag aatatgttca cctccagggg cgctccaacc
     1321 tttctcgcga gatcgaccat ctcgccgggg acgagggcgg cggcgttccc gttgacgctg
     1381 aggaccggcc tctccgccag gaggaggagg gccgccgccg cccgtgccgc ctcctcggcg
     1441 ggaggggtcg tcacctcgcc gaggaggtag tcgagggcct cgcccctacc ttgcgcgatg
     1501 aggccgttca ggccggtgat ccccctctcg accccctcca ccaacttctc ccgggtcagg
     1561 agggaggcgt acctcggatg ggatgggggg acgtcactca agagaggcac ccccaaggct
     1621 caccctcgct ctgatcacat ctccaaactc cgccaaggcg tcccctccgt tgagggcgaa
     1681 gatcgcatcc cccagcatca ccatgctggc cattcctccc cgggactcca ccgcttcgac
     1741 ggcgtcctcc atccatcccc gggcgagccc cgcccttcgg gcgaactccc aggagagggt
     1801 gacgaacctc tccagggtcg gcctcttcag aagctcccgg agggcccgct ccccctcccg
     1861 gttcaccccc ctcatcgtcc cggcgtccga gaggacctcc ttcgtcgaga gggggcctct
     1921 caccagaagc tccacctcca ggggcgggac cgggatcctg tcgacgaccc ccagccccgg
     1981 agcccccggg gagagccgga ccaccaggcc gccggtgttc tgggccagga cgtcccccag
     2041 gccggtcccg gaggcgacct ccgccacgtg ggcggcctcg ccgacggcgt tggcggtgag
     2101 gccgaggcca aaggccccgt tgatggcgta ggcagagctg agggcccccg ccccgctcgc
     2161 cccgaagccg gaacccaggg gcatagagag ctccgtctcc acccggacgg gggagggggc
     2221 cagggtatcg acgaccctcc tcgtcaccgg cgcctccgag accaccccgt tgaggaggac
     2281 ggccgtctcc tccgacctag atacggtggt ggcggccgaa aggtccaggt tcaggccgcc
     2341 gccgacggag cccgcccgcc tcgggtcggg atcggggcgg gcggcgaaga atcccgtgat
     2401 gtgggcgggg gcggaggctc tcatgatagc tcccccgcca gggcgtcgac gacggcttcc
     2461 gcgatcttcc tcttctttcc ggcgacctcg gcgacccccc ccgaccggga taggatcagg
     2521 acccggttat cctccgtccc catcccgcct ttgccgacgt cgtttccgac gacgatgtcg
     2581 aggcggttct tctccatcga ctcccgggcc cgaaagatca gctcctcgtc ggataggaag
     2641 gtctcggcct tgaaacccac catcttgatc tcggggtggc tcgccctgac ggcggcgatg
     2701 atcttcgagg tcggcctcag ccggaggacc agctcccccc cggactttat cttatcggcg
     2761 gaagggtcga gggtgtagtc ggagacggca gcggcggcga cgatggcgtc gtagccgcct
     2821 ctctccagct ccgccaggac cgcccggagc atatcctcgg cgctctcgac gtacacctcc
     2881 ttcaggggga ggccgaggcg gcctcgatgg acgagggtca cctcggctcc gcgcctccgc
     2941 gcctccaggg cgatctccgc gcccgtcttc cccgaggccc ggttcgtcag aatccggatg
     3001 ggatcgaccc tctcggcggt ggcgcccccc gtcaccagga ccctcctccc ggcgaggtcg
     3061 ttgggtccga ggagcctctc gacctccagg accaccgtcc tgctctcggc gatcttcgcc
     3121 ttctcctcct cgatccgggg gtcgaggacg acgaccccca tcgaccggag ggcctccagg
     3181 ttcctcatga cggcggggtg gcggtacatc gcctcgtgca tggcggggac gacgacgacg
     3241 gggacgccgg agccgagggc ggtggtggcg taggtggtga cgggggtgtc gtcgatgccc
     3301 caggcgatct ttcctatggt gttggcggtg gccggagcga tcaggaggag gtcggccctc
     3361 cctccgacgc cgcagaactc gacgtgctcg accctccccg tgatcctcgt caccacgggg
     3421 tttccgctcg catactccag ggcgtgaggg tggaggatct ccgtggcagc tccgctcatg
     3481 acggagtgga cagccgcccc ccgccggatc aggtcgcggg cgaggtcgac ggtcctcacc
     3541 gcggcgatgg accccgtcac cgcgaggacg atcaacttcc ccgataggga atcgctcacc
     3601 gtgcaggata tggtcgaagt catctcggtc agccgcccat atccagcccc ccttagaaat
     3661 aggttctccc tcggcggatg agcacaacgt ttatctaaca aatcacccta ggggatctgc
     3721 tgtgccgggg tggcctagct ggttagggcg nncgaaaatg aggcgctcgt ctcctgaggc
     3781 atctggaagt cacgggttcg gatcccgttc ccggcaccta acttttacca tcctgccacc
     3841 gggcaagggt actcgcctat gggtggagga taggcggaga aaaaaaccgt cattttcgat
     3901 tttcaaacct cacgtggccg gttcgacgcc ggagatggag agaaatcccg atacaatttg
     3961 gcagattcgc ccccttcgac cttaacaggt acctttaagt cgatggattc cgaatgatta
     4021 accggagtcc tgtcgatgcg aatcggagtt tacgtctgtc actgtggcct gaacattgca
     4081 ggggttctgg agccctccgc cctcgccgag ttcgccaaaa ccctccctgg ggtcgaggtc
     4141 gcccgggacc tccagttcac ctgctctgat acgggccagg atatgatcaa ggaggacatc
     4201 gcggagcata agctggagag catcgtcgtc gccgcctgct cccctcgcct ccacgagccg
     4261 accttccggc gggtgatctc cgaggcgggg atcaacccct tcatgctgga gatggcgaac
     4321 atccgggagc agtgctcctg ggtccacatg gacgagcccc cccaggctca ggagaaggca
     4381 aaagacctga tccggatggc ggtggcgaag gcgaccctcc taaaccccct ccagggggag
     4441 acgatgcccg tcagccggga ggtgctggtc atcggcgggg gggtcgccgg gatccaggcc
     4501 tctctcgatc tggccgactg cggcctctcc gtccacctcg tcgagaggag gccgacgatc
     4561 gggggttaca tggccctcct caccgacgtc tttccgacga acgactgctc catctgcgtc
     4621 ctcgcgccga agatgaccga cgtctacaac caccccctca tcgacctcgt cacctacgcc
     4681 gagatcctca acatcgaggg gtccgtgggg aggttcaccg tatccggggt caggaaggcg
     4741 cggttcgtcg acgagaagct ctgcaagggc tgcctcaacg agtgcgccgg ggtctgtccc
     4801 gtggaggtcc ccgacgacta cgagttcggc ctcggcaaaa gaaaggcgat ctacatgccg
     4861 ataccccagg cggtccccct ggtcgcctgc atcgatccct cggcctgcat cggctgcggc
     4921 ctctgcgccg aggcctgccc cgtcgacgcc gtcaagtacg accagaggca ggaggagttc
     4981 aggttcgacg tcggcgccat catcgtcgcc accggctggc agtccttcga ccccgccaga
     5041 aaggaggagt acggctacgg caggtataga gacgtcatat ccgccctcca ggtcgagagg
     5101 ctcctcaacg ccgcagggcc gaccggcggc gaggtggtgc ggccatcgac gggggaggtc
     5161 gcgaagtccg tcgccttcct ccagtgcgtc ggctcccggg acgagacggt cggaaacgca
     5221 tactgttccc gggtctgctg catgtacgcc ctcaagaacg cccagctgat caaggagaag
     5281 tacccggaga cggaggtctc gatccactac atcgacctcc gggcgggggg cgaggggtac
     5341 gaggagttct acatacgggc ccagcggctc gggatcaact tcatccgggg ccgggtctcc
     5401 gaggtggagg aggtggacgg gagccttcgc gtcaactacg aggacaccct cctggggggc
     5461 ttccgttcga agccctacga cctcctcgtc ctctcggcgg ggctcgagcc gaataaggat
     5521 gccgacgtcg tcggcaacct cctcggcctc gcaaagcgcc ccgacggctt cttcgagatc
     5581 gcccacccca agatgaggcc ggtggaggcc cacatcgagg gggtcttcat cgccggttgc
     5641 gcctcgggac caaaggagat ccaggtctcc atcgcccagg gggaggcggc ggcggcgaag
     5701 gcgatgaggc ttcttgttag aggagagctc gccctcgacc ccgtcgtcgc cgtggtggac
     5761 caggagaagt gcataggctg caagctctgc gtcgatacct gtccggggaa ggctatctcc
     5821 gtcaacggga ccgccctcgt cgacgaggcg gcctgcaagg ggtgcgggac ctgcgccgcc
     5881 gcctgccccg tcgacgccat cgatatgacc ctcttctccg acgagcagat catggcccag
     5941 gtccgggcgg cgacggcggt gaaggggcag taccccttca tcgtcgggtt cctctgcaat
     6001 tggtgcagct acgccggggc ggatctggcc ggcacctccc ggatccagta tccgacgaat
     6061 atgagggcga tccgggtgat gtgcgccggg agggtcgacc ccgccttcgt cctcgaggcc
     6121 ctgaaggggg gtgccgacgg cgttctcata tccggctgcc gcctcggcga gtgccactac
     6181 aacaagggga actaccaggc ctaccagcgg gtccaggtcc tcaggggggt cctggagaag
     6241 gtggggatca accccgggag gatcaagatc atctggtgcg ccgcgagcga gggcgagatc
     6301 cttgcaaaag aggtcaggaa gttcgtcagc gagctgaagg agatggggcc ggcgggatca
     6361 gagctcgctg ctctcaggcc cccgaagcct gtaggaggcg gttcctgatg tcgatggaga
     6421 tcgagaagga gatggaggtg gagggctcca ccatcaggtc gatccagagg tcggaggact
     6481 cggtgaaggt cctggactac gactacaaga agtgcaacgg atgcgggatc tgcgtcgccc
     6541 tctgtccggt aaacgcccta cagctcggcc ccgtcatcga gatcgccacc ggcctcgacg
     6601 cccccccggt cctcatcgac ctcgataaat gcgtcttctg cgggatgtgc gccgccttct
     6661 gccccgtcga cgcctacagg atgaccgcca acgaccggga ctacaaagag atggaggagt
     6721 acccccacct cgtctccagg gccgagccga acgagaagtg tcttccatgc gccctatgcg
     6781 agccggtctg cccgacggag gcgatcaccg tcgtctttta cccgacaagg gaggtcttcg
     6841 gccccctccg ggaggaggag aggggggagg ggaaggggaa gatcgaggtc gaccccgaga
     6901 agtgcaacct ctgtggtaag tgctccaagt tctgcgaggc cttcctcctc caggagaagg
     6961 agcccacccc caccgacctc gtccccttcg agcagctcct ggtggacgag gagctctgcg
     7021 accactgcgg cctctgcgtc gggatatgcc ccgaggaggc gatcaaggtg gaggggaagc
     7081 ccctggagct ggaggaagag tttgcattct ccggggagat cgaggtcgac caggataagt
     7141 gcatcggctg cggcaggtgc ctcctcgtct gcccctatga agccatggac gtcaccaagc
     7201 ccttcgaggg ggagataagg ctcgtcgagc ggcagctgcc actatgcgac cccatgggct
     7261 gccacgcctg cttcaacgtc tgccccgccg actgctggta tgtcggggag gacggcaaga
     7321 taaaggtgga gagggatcag tgcatcttct gcggggcctg cgccaactct tgccactgtt
     7381 tcgccgtgga ggtggatcgc tcggcggtga cccacacccc gattatggag acgccctggg
     7441 cggaggagtg gaagaaggcg atctcctcca tcgtctccaa atcgaggagg cggccggacc
     7501 tctcgggggt cgtcctgccg ccccagatcg agcggatccc gatacccgag atcaaggcgc
     7561 c
//
LOCUS       DASM01000042            5833 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_138472, whole genome shotgun sequence.
ACCESSION   DASM01000042 DASM01000000
VERSION     DASM01000042.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_138472"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          886 a         1945 c         1574 g         1428 t
ORIGIN      
        1 ccatcgcctt catcctcttg ccgaagagga ggccggtgag gtatgccgcc gggacgttcc
       61 cggtgttctg atcaaacccg agcttcttca gctcctggga gttgaccgtc aggagggtcc
      121 tgtctccgac cggctccgcg acgatgagct ggagggagat gttcttgttc ccctttctga
      181 tcacaagcct gggctttctc gaaaggatga gcttgtatcg gaagtgatag tccgttctgc
      241 cctctctccg ccttctgaag ggcactttgt atcgtggtcc ggttgccatc agtcactcct
      301 cgttagacct tttgccttga tgtaagcgtt gagatgggct gtgctgcggt actcgccgcc
      361 cttggccttc cgatagagca tccgataggt gtggcgatcg atctcgcctt cttccctcag
      421 ctctttcagg ttgctgcgaa gggcccggat cttggtcatc cagaccactt tcctcggaga
      481 ccgggaacct ttcgcgccct ttctcctccc ctgtcccttc tggtggccgt aggccctctt
      541 ggcggctcga accctgtatc tagcgcggct gttccccttc ttcggcttgg ccttgatatt
      601 tccagcctcg atctgagacc tgatgtcctc cctcgtgatg gcgtcggcga tctcgccggc
      661 gacctccggg tcggggttta tccagatcct gctctccccc acggcgagga ccgacgctgc
      721 aagcctcttc tgcgttgaga agtccggcaa ctcagttccc tcccgcggtg gggttgagaa
      781 ccttcaggtt catctcccgg gccatagctt cgatctcgtc tctcttcttc atgccgacgg
      841 tggcggcgat ccggacggcg cagccctcgg cgttcttcag gtcgtctgga ttcttcacca
      901 ggacctcctt catgccgcag gggtgatacc ccctgacgcc cctggggctg ccgtatccgg
      961 ccttcaccag cttccccttg gcggcgatct gctgtctcag cttgttgtgg aggccccggg
     1021 gccgcctcca gctgggcgag agcttcttct tcttgtggga atcgcaacgg ttgaactcgg
     1081 gcttcctctt cttctgctta gcccgaactt tcatcaacct ctctctctcg gaagtcaact
     1141 ccgtcacctc ttatccacca gatatatccc gtcctggaat actctgatgt cgaatcctct
     1201 tactttggtc gcctgctcta tgttggccat cgtctggccc accagctcct tgtcgattcc
     1261 ggtgacgatc acggtgtcgc ccttgatctc cacctgggat ccgcccatga tcttggcggt
     1321 ccggggcttc ctctcgccca ggaagttgct gatcaagagc tctcccttgg aggccttcag
     1381 ctgaatgggg aagtgagagt agacggcctt catcctgtac tcgaacccct cgttgacccc
     1441 cttgatcatg ttcttcaggt gggcggctat ggtccctgcc atcgacctgt gccgcttcct
     1501 ggtgatctcg gcgtccacca ccagggtgga gtcttccttt cggatctcta tccctggata
     1561 ccagagccgt ctcgagaccg tccccagggg gccggtcacc gtcaccgtag cgtcgtcgcc
     1621 gatctccacg gcgacgccct ctggaatctc aataattcgc gccaactcct tgaccatcgt
     1681 gaccacctaa tagacgtaag ccagaagctg gccgccgatc ccgcgctccc tggcgtcgac
     1741 gtgagatatg accccctggc tcgtgctcat gatgagcacc ccgaagttcc gggaggggag
     1801 gaacctcttc tcccacttct ccaggtcggc gacctgggtg gagaaccgag gcttgatcac
     1861 gccgcatcgg ttgatcttcc cctggaggtt gaccttgaac atcccggact tgccatcgtc
     1921 gacgaactcg aaatcgccga tgtatccgag gtcagccatc accttcaggg tgttgccgat
     1981 gagcttggaa gcgggctgga tgatgcatcc ccctttgccg acgtcctcag cgttcttgat
     2041 gatggacatg gcatcggaaa gcggatctga cagtaccatc aatcatcacc tacgagtact
     2101 tctcaaaacc catctctggg gcgatctctc tgaaacactg cctgcagaga tagattccgt
     2161 attttctgat gagcccaggc cttctgccac accttttaca ggcatttgcg ccccggccga
     2221 agctcttggt atcgctcttc atgccgcctc cacgatgtca acgccgtatt tattcttgac
     2281 gtactcgacg gcgtcttctc tcgtcactct gtgctttgag gccatcttcg ccctggcgac
     2341 ccgcctccgg gccacccggt atcccggccg cttcagggcc accgagacgt ccatcccgaa
     2401 gatcccgatg tcgggctcgt acttcatccc cggaaagtcg gtgtgctcct cgaccccgaa
     2461 ggagaagttg ccggtatcgt cgaaggagct ccgccggagg gcgctccccg ccacctccag
     2521 ggccaccttg aggaactcct cggcgtctct cccccggagg gtgacccggc atccgatcgg
     2581 ctccttcttc tttatcccga agcccggcag ggtctttttg gcgatggacc tgacggggga
     2641 ccggctggtg atctcgccga ggatagtctc ggcgttgacc agccgctggc cgctctcgcc
     2701 ggttccgatg tggaccgtca ccttgtcgat ccgcggctcc aacatgacgt tcatatctcc
     2761 gcccccagct tgatggcggg cttatctctg ccgatcatga agacgtggtc tacggtcgtc
     2821 tcgaactcct ctttcccgga gatgatgacc cggttcggtc gagagctctt gaccacctcg
     2881 atcaccttga tcgtaccgac ctccccggag tgggtgccgc cgacgaccat ggcgaggttt
     2941 ccttccttga actcgatgac gtcggagatc tccttatcgg gtatcgatag gatcaccgag
     3001 cttcgggtgg tgtagtcgcc ctcggccagg atgttcgttc cgtcgttgag gttcagctgg
     3061 gtcttgccgc cctggaggac cgtcttcttc tcgatcctgc agagcttcct cgcctcgccg
     3121 gcctccagct cgtggaggtt gaacctgcct ttcgcgtccg taaggagcct gaaatttttg
     3181 tccagagccg ggaccgatat cacgtcgaag atccccacgg ggaagttgag gtctttcctc
     3241 gcctttccgt cgacgaggac cccgccctca tggaggacgc gcttcgcctc tctggagttg
     3301 tcgacgagcc ccaagaggtc tctgactacg acgacgaggg ggatgctccc cctctcagag
     3361 tgggggcctg gtctcgtctt ggccacccac gtatggacct tccttgcgat cggccagctt
     3421 ctggggctcg caaccctctt ctgatgtctg ctcacctaaa tcacctggaa aagatcttct
     3481 tccgctcctc gtcgtccatc tccatcttgg tgatgatgac gttggagggc tggactggcc
     3541 tgggcacctc ggtcccgtcg gcccggaata cgctgactcc ggcgacgtgg atggtgccgc
     3601 tcttcagatc cgccttctgg acctctccct cggtccccgc ttggtcgccg cgcatcagct
     3661 ggacggtatc gcccttcttg acctgagcgc tgcgggcctt catctcctcc cggagctctt
     3721 tggagagggc ggcgtgcatg tacttctgcc gtttgtgaag gggggcggag aacctctctt
     3781 ttctctgctt gcgtggctgt ttgctcttca tggtgatcac actattatcg aggccgccga
     3841 ggcgatcttt ccgtacctct cagccacctc tcgggccact ggccccttta tctccgatcc
     3901 cttgggaatc cctagagcgt cggtgatgac cgccgcgttg tcctcgaact tgaccctcat
     3961 cccgtcgggc ctccggaact ccttcctctg gcggacgatc accgcggtca tgatctgctt
     4021 tctcatctcc ggggtaccct tcttgacgga taccactatc atgtcgccga ctccagcgca
     4081 gggcatccgg ttctttacgc ccctgtacct cttcaccgat atgatctcga gcaccctcgc
     4141 gccggtgttg tcggcgcatt cgagccgcgc tcctgcgttg atcggccttg agatgctcgc
     4201 cttcaacccc ttcatcggac gacctccacc acgacgaagg acttggtctt gctgaggggc
     4261 ctgcactcgg cgaccttgac gacgtcgccg accttggcct taaggcaggg ggggttgtgg
     4321 gcgtggagct tcgaccgcct cttctcgtac ctgtcgtact tcgtcatctt cctctcgaac
     4381 tctcgcagga ccaccacagt atcggtcatc ttgtcgctga ccacctttcc gtcaaagacc
     4441 tgaccgcgaa cggaaagctc tccatgaaag ggacattttg gatcgtcaca ggccttttca
     4501 ggggacttga cgcccagtcc gacatccctc ataatttcca cctcgatctc cgtatttttt
     4561 tgttaatcct gttttcgggt tgtgagacta aaactgagcc ctctgccttc accttcaggc
     4621 cctgggacag ggtaaaaatg aagcagttgc aggacttggg aacgcatttc actccgctcc
     4681 tggtctcgat cagaaaggtg ttccgggtct catcgacgac ccggccggag atccccgcct
     4741 gggcggggtt agtccccctt gcaacctcca ccggaaggcc gatgagctca tgtctgacga
     4801 gattctcagg agtgatcatc acgccgttgc acgctccttc tgaatggtct tgatcctggc
     4861 tatggacctc tttatctccc ttatcctgcc ggggttctcc ggggcgcccc cagagacgac
     4921 gagcccccgc tcccggacga gctcgccctt tagcttctca agctcctccc ccagctcctc
     4981 ggggctcatg ttcctcagtt catcgaccct gaatatcgcc atgtcaagtc cctctcgtct
     5041 cacccttctc tcttcggctc ttcagaggcc gcctcggcgg gggcctcctc ctcggtggcc
     5101 ggttccgcgg agaccggctc ttcggagatc ggctcgtcgg attccgcagc aggctcctcg
     5161 gaggccttct cctcgaaaac cggctcttcg gcgggggtct cttcgatggc aggctcggcg
     5221 gggatctcct cctcgacgat ctccatctcc tcgaccgtca cctcgacggc gccctcggcc
     5281 ccggactcca ggagctcctc cagctcgtcc ctcttctcct ccactggaag ggcctcttca
     5341 gcctgttcct tggagggggg cacgatctcg aacctgtcgg ggggcacggc gtcggggggg
     5401 acgatcctca cctggcatcc gatggtcccg agcttcttca ccgccacggc gtagccgtgg
     5461 tcgactatct cctgggaggg cttccccgaa tgcttgacgt agccggagag gaacttctcg
     5521 accctcgacc tcggtcctgt gagcttgccg ctgaggatta tctcgcagcc gagggcaccg
     5581 ctctccatta ccctccgcaa ggtggtctgg cccgcctttc tgaagtacca gcccctctcc
     5641 agggagctcg cgagcctgtt ggccaccacc tgggcgttca gctcggcctt tcccacgtcc
     5701 tgaacgtcga tctgggggtt gtccagccgg aagtttcggt ccaggtctcg ggtgagcttt
     5761 cggatcaatt tgcccccctt gccgatgacc attccaggct tttcggcata gacggtgatc
     5821 tggatcccca tgg
//
LOCUS       DASM01000043           18351 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_42949, whole genome shotgun sequence.
ACCESSION   DASM01000043 DASM01000000
VERSION     DASM01000043.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 18351)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18351)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18351
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_42949"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         3330 a         5953 c         5859 g         3209 t
ORIGIN      
        1 aggagacgaa gtactcgacc cggttatagg ggaagaactc ccgaaactcg ttatagagct
       61 gggccgccag ggtcttgttg tgggcgatga ccagggtcgg cttcttgacg gcgtcgatga
      121 ccctggccac ggtgtaggtc ttgccggagc cggtgacccc gaggagggtc tggaggcgct
      181 gcccggattc aaggccgccg acgagcttat cgatcgcctc gggctgggag cccttcggct
      241 cgaagtcgga ggcgatggca aactctggct ttgtaggtgg atcagaagat tgagcctgtc
      301 tcgtcatggc tgccgggtcg gagattcttt caggggagag gtggaaagga ggggtttacc
      361 gcctcccagc ctcacccccc tttcgcctct ttaaggtact ccagggtcct cttcttcatc
      421 tcgcgagcct cggcaccggt ggggtcgagg aggatcgccc ggtcgaagga ctccaccgcc
      481 tcctcatacc tctccaggcc gatgagggct atccccctca ccatccagcc ggggacgaag
      541 tcgggcatcg catcgacgag ttcctcggcg aggtcggcga cgtcctcgta cctcttcagc
      601 ttggagaggg ggccgagctt gttgaacttg tagctcggct cctccggctc cagatccaga
      661 aggaagtcga ggaactcgat cgtcgcctgc cagtctcccc ggtcaaagat cgcccccgcg
      721 aggtcgttcc actcctcggg atcccggagg gtcggggcta cgccgacacc ctgccgatat
      781 gcctccttcg cctcctcgat ccggtcgagg tttcgcaagg tcatgatctt cgagatccag
      841 ccccgcaggt ggtcgggctt cgctgcgatc gccctatcga agtactccag ggctccttcg
      901 tcgtcgttct tgagggagga tatccccccg aggaggtcga ggatctccgg gtcttcgggg
      961 cggaggtcga aggcccgctc ggcgctggct cgcccctcat cgagcctccc cgcccccagg
     1021 tacgccaccg ccagcttgta ccacccctcg acgtcggagg ggtccttctc gacgatctcc
     1081 ccgaagaact ccaccgcctc ctcgaacctg tccgtatgca tcaggaagat gccctcagta
     1141 aacctgtctt ctcccatcct gctcgcccct ccgtggccct gcctcagacc ttctcatcca
     1201 cttctcctcc ggcatccttc aaacagatcc ggtctgtacc catattataa aagcctcttt
     1261 ccccgcccga cggggcgatc ggccggccgc caccatccgg agcctccgat ctaggtgggt
     1321 ggggctccat ctcccgcctc cgccaccagc cacgccgagg cgtcgagata gacccccctg
     1381 ccaaaaaagg cgtcccccga ggaggcctct ccgaccgacc gcctcgccct ccgcttcaac
     1441 accaccatcg gcggctcggg gaagggctcc gatagggacc gatgctcccg gacgatctcc
     1501 tcgatctttt cctcgtcgac gaggccgatc cggtcgatca cctccacctg ccgctggaac
     1561 cggtcgatcg cctccgtcga caggttctcg atgaagggga tcgcccccct cgaccccacg
     1621 atcctcccgt ccgggtcgat cccgtttttg tggagggcga cgatggagtc tccggggagg
     1681 tggcccttcg actcctctcc gcagaccagg aggaaccgga tctcggagtt cgagatgaga
     1741 tttccgacga ccttctcgac ccccaggttc tcagtcttgc aggggcccca gatcgccgcc
     1801 cccggagcgc ggaggctgct cgccagggtc accaccgcga tcctcgagcc ggggtcgccg
     1861 aggctgtact cccccctcaa tggaggccat cttcccggtt catcccccct cccccctccc
     1921 ctctcctccc tcctagcccc ttcggcgcgg tcatcttcat ccgaaacgac gggatgttcg
     1981 caggtcatcg taagagagat ggactccagg gtttaaaagc tttgaaccgg ggtagcacgc
     2041 cggcttggga gcacctcaac aagaggctta tatacaaaaa gggctcacct gatgattaat
     2101 tgggaaagtt cggcggaccg ggatggacgg tcgccgaggc aggggctgag agatatggca
     2161 tcttacgagg agcggatcct ccaggcgata gaggattcgg aggtggggct caccaccgtg
     2221 gacgtggcga aggtggcggg ggtgagcaag acgacggcga taaagtacct ctcagtcctc
     2281 aagtccgaag ggaaatgcga gtttgttgag gtcggcccct ccaagctctg gaggaggaga
     2341 aaactgcccg gagatctcag cgaggccgga gaagggctga agagcctgga ggccgccctg
     2401 aggcggggca gagccgccga ggcaccggtg aacttatcga tcatagcccg cctcgaggag
     2461 atctccgagg atctgaccgt ctccctctcc ttcaaggtca ggcccgagaa ggtcgaggcc
     2521 ctcatcgacc tgatcagagg agaccttccc gagaaggagg ggctatagcc ccggcgggac
     2581 ccggaaatta gccttatata agatatggga taacaaatag ctctaaaatg gtggattcaa
     2641 tttatgcgga taccttctga gatccacgac ttcttccggg tggaggaggg gcagaccctc
     2701 ctcatcaagg ggatgccagg caccgggaag acgaccctgg ccctggagat catgagctcc
     2761 gtctgcgaga gggagaacgg gatgtacatc tccacccggg tcagccccgg ccgggtccac
     2821 gccatgttcc cctggatcgg cgatatcatc ccctccgcca acgtcgtcaa cgccacccag
     2881 aagatgctcc tcgaaagcct caggaatgtg ggccgggagg ggtccagcta cgagacggtc
     2941 ctcgacttct tcaagacctt cctcgaccag gccggggata tggacgaccc catgatagtc
     3001 atcgacagct gggacgccat cataaactac accgccgcca tcctcggcgg cgcccagcac
     3061 agcctggagc agaacatctg cgagttcgcc cgggatatgg ggatccacct gatcttcgtc
     3121 agcgagtcgg cggagctcct ccccctcgac tacatcgtgg acggcgtcgt caccctcaag
     3181 aacgtcagga tggcagcccc cctctccggg gagaaccgcc ccgaaggcat gacgacccgg
     3241 tactcccggg agatcaggct ggacaagctg aggggggtgg agataaagca gaagatatac
     3301 acctgcaccc tccacgacgg aaggttctgc tactttgagc ccgccctcga gaacctcagc
     3361 ccccagatcg ggatcgtctg cgacctggag aggatcgccg acccccggga ggactccatc
     3421 tccaccggga tcggcgactt cgacaggatc acgggggggc tcgacttcgg atcctgcaac
     3481 gtcctggaga tcgatcacgg cgtcggcaag aggtactatc aggtcctgac ggccctggct
     3541 tccaactccg tcaagaacgg cagggcggtc cacatcgttc cgagcatagg ctaccagctc
     3601 gcccccagag acgtcttcgt cccctcgaac gtgatggtcc tggagccggg gggggaccgg
     3661 gaccagtggt acgagaccct cttcaaacac tgggactccc tccgggagag gacgggccgg
     3721 cctatcctca acatcctggg gctcgactcg atggagtttg ccttcggcta cgagaggatg
     3781 ctcaactcca ccagccggat gttccagaag tggaaggaga cgagcgacgt caacgtggtg
     3841 gtggtgaagt cggggcagaa gagcatccgg atgacctccc acatcgccga cacctacttc
     3901 gtcatcaagg agctgaacgg ggggctctgc ctctacggcc tgataccccg gaccgagccc
     3961 cactacatgg tcagagacga tagaggaaac ctccgcctcg ttccgatagt ctgatgctgg
     4021 cgtgggcctc tctgaggctc tggcctccgg aaggcaggag accccgaacc ttcagaccca
     4081 cctctcgccg ccgtctattc tgatctcctt cttccagatc ggagcccgcc tctttatctc
     4141 ctccagtacg aactcgcacc cccggaaggc ggcctgccgg tgaggtgcgg tggcgacgat
     4201 gaggactata tcctccccca ccccgagacg gccgacccgg tggacgacgt ctaaggactt
     4261 gaggtcaaag gtcgccatcg cctcgtcccg gatcctcgcc agctcctcct ccgccacctc
     4321 ccggtaggcc tccacctcga ccccggatat cccgtcgtcc ctgacggtcc cgaggaaggc
     4381 gacgaccgcc ccggtgtcgg gccgcttcgc cccggccacc agatcctcga gggagagctc
     4441 ccggtcggtt atctcaatca ccataatacc tcaccatgga gccggaagaa tctcatcctc
     4501 cggagaaggg ggggaggatc gccacctcgt ccccctcctc caggaccgtc cttcggaggt
     4561 cggaggggtc tactccccgc cggttcagca ggaccgtcac ccctccgccg cctgccctct
     4621 ccagctcgga taggactttc tcaaggccgg ggtgggcgga ggctacctcc tccaggaggt
     4681 cggcgagggt ggacccctcc gggaggtcga aggccgcctc ttcccccacc acctcccgga
     4741 acctcgcgaa gctcctcacc accaccctca tcccttcgac ctcagcctcc cgatctcgtc
     4801 caacgcctcc accctcagga cgaaggtccc gtagcagggc ttggtatagg ggaagatcac
     4861 cctatcccca tcgatcccga tgaggagggg ggggaccccg atcagatgga cgttcaggat
     4921 cctgtacccg ggtgggacct cgacggtatc cttgaagttc ttctttatgt actctctcgc
     4981 ctctttgaag gagcactggg ataggaggac ctcatacttc atctcctcta ggcagcccaa
     5041 tcagatcacc tcatctaaaa gcctcttcag gggcaggggg tcatggacgg ccttggcgac
     5101 tagctttctg cagtcgaact ccaccgtctc cgccagctcc tcggcggacc taccgaagat
     5161 caggacgaag agcagagccc tggagatgta gctgatcgtc ccggcgtagg ttataccggc
     5221 gtcgctgtgg gcgaaggcga agcactggtc gaagtcggcc ttaccctccg atatggaggc
     5281 gatgcaccgg tcgatgttcg tcgtctcccc gaggtagtgg cccagggggt ctgcgagctt
     5341 cgtaagcttg agggcggcgc cggtacccgc caccaccacc tcgtcccccc tctccctcag
     5401 cctataagcg aggtagaggg cgatgctggt ctggacgggg acctcggggc agccgaggag
     5461 gatgagggac cgcctccttt cgggcgaaac tccaccagaa ctatccatgc tagaccccat
     5521 ccgccccggg gctacttaaa gctctcttcg ccggccgcag tccgggcagc cggggttcct
     5581 ctccaggggg atctcgtcgc acctcccccg aaagccgtcc cagaggagga gcctccccgc
     5641 cagggacgcc cccagacccg tcaggtgctt gacgacctcc gtcgcctgga ttgagccgac
     5701 gatcccgcag gtggcgccga gggcggggac cttctcctcc ggcggcggcc gggggaagat
     5761 gcaccggagg catggggtcg cgccggggac gatcgtcgtg gcctggccgt cgaggccgga
     5821 gatcgccccg tggaataggg ggacgccggc ggcaagggcc gccccgttga ggaggtgcct
     5881 ggaggagaag ttgtcgaggg cgtccaccac tgcgtccgcc ttcgccacca gccccggcag
     5941 gttccccccg tcggcgaaga gggggacctc ctccacctcc agctcggggt taagcccccg
     6001 gagggaatcg gccgccgagg cggccttggg acggcccaag tcctcctgcc agtggaggat
     6061 ctgccggttg aggttgctca cctcgacgac gtcgccgtcg acgacgacga tcctccccac
     6121 ccctgcgacg gccagataga ccgagacggc ggagccgagc ccccccgctc cgacgacgag
     6181 ggccgtcgac ctcgcaagcc tcctctgccc ctcctccccg aagaggggga tctgccggtc
     6241 gtacctcgcc gcgtccgtca actatcgcac ctccttgatg gatgatggga ctgcccgctt
     6301 cagcccccca ccaccgccgg gaagagggcg acctcgtccc cctccgccag cggcgtctcc
     6361 tccccggcca gggatagaaa gttcctcccg ttgaccatga cgttcaagtc ctcccggacc
     6421 cttccagagt cgtcgaagag ctggccccgg agctctccgg accgggaggc gaggctctcc
     6481 agaaggtcgg agacggcggc cccctcagca agcttgacct caaactccga cccgaggagg
     6541 gccctatacc gggcaaaggc ctttatcctg atcagcatga ccgctccgcc atctccttga
     6601 tcctctccag ggcccggagg gcgtcctccc tcttgaggtc cctccgctcg tcgcggagga
     6661 acctcaaaaa cccttcccgg ttgcctccat cgaggtccgc cgccgatatg atctgatcgt
     6721 agctcctctt ggggaagtag agggatgagg ccgccgccag gatcttctcc gcggagacgg
     6781 cgtcgatcgc ccccctctcc ctggcggccc ggacgttctc cctgacgttc accaggggct
     6841 cggataaggg agcgaagctg atcggatcga agattagggc cacctcgtcg tcggagatga
     6901 taaacccatc ccggtagagg cggaagatct ccccgacccc ctccatcccg aagctcgcga
     6961 gctccgccgc ccggagcgcc cccatgctgg aggcgccgac gacggcgacc cccatctcca
     7021 gggcccggag gacctccttg tgggcgacgg cgctctcctg gaagaagacc ccgtcgatca
     7081 gccccacgat cctcgccccc tcctccgccg ccctcaagag gtcgcccctc gccgcgggag
     7141 gccggtagtc ggcccggagg accatctcgg ccgaggccct atcgaggctc ggcccgagga
     7201 agacgacgat ttctcttgac ctcatctctc caccgtctcc ccctcctgtc ggggtccatg
     7261 gcgctcatct ccaggcccgg gacgacgacc cggaccaccg ggacgccgat ctccgggcgg
     7321 gtgaggtcga cgactatggc tctatccaaa ccggcatctc cgagccgctt cagcatgagg
     7381 ttgatgtcgt cgaggaagtc gtcggagtcc tgggacctga tctctcggaa tggctcggtc
     7441 ccggtggact caaaccagtg gcggttcagc ctcttcgtcc tctcgtaccc gatctgcctc
     7501 cggaagtccg ccgtcgtcgt gtcctcccta gccccgtgga tctgggtcag cctcgactgg
     7561 gcgacctccg tcagggcccg gaggacggcc acctcggcgt tggtgtgggt ccccatcccg
     7621 atggtgagga gggcagggtc cttcagcttc aggtcgtcgg aggcagcggc gatggtggga
     7681 accccaatgt cgctggtgat gtccttgatc ctcacctcca cctccgcctg ggcgaacctg
     7741 tccaggaggg agcgggcgag gccgtcctcc acctccgtga ccaggggccc cgtcctcttc
     7801 accgcctcgg cgagggacca ggcgtcccgc tctatcacct cggagaggcc gtggaagacc
     7861 gcctcctcca ggacgttccc ggaggcgagg ccgttggtgt tcgtcctgaa gagctgcggg
     7921 tactccctcg ggagcggatg gaagacggcg ctcgccggga gcatcacctc ctcctcgccg
     7981 atcaggtcgt agccgaggac ccagggcatc ggcatcccgg ggtccgcccc gtcggggagg
     8041 gcgaggtccc tggggtcgac ggcctcgacc ccggctgaga gcctcgaatg gctctccacc
     8101 aggagctgcc tatcgtggac ctcggcggag taccgctcta tcccctccat catcgctgag
     8161 accctcgcct cggcgggggt cgcgcccttg ccgttgtaga cggatatcgc ccccctctcc
     8221 gctgcgggcc tgatgctgga gaagaccggg atcccgatcc ggtccaggtt ggttatatct
     8281 gccaccctgg tgatccctgc gagctccatc ttgggggcta tccgctcgag ggtctcctcg
     8341 ggagaaaaag ctcgatgggt gtcggtcttg tacttcttta ggcacgatct gagcatcatc
     8401 cggtatcaac tcttgagatt gggaccgttc tggacctttg aatcccctgg ccctggtggt
     8461 cttggggccg gtcctcctgg cagatccggg acggggggag cgccagcgcc ccggggaact
     8521 catccctctg cccctaaata catgtcgggt ttctccccgg ccctccgata gaatggatgg
     8581 agactcagag gagagataaa aagatccagt agacgaggag gtttgcgacc gtcagggggg
     8641 cccccacctt catgaagtcg gtgaaggtga gggccgcccc cctccgctcc ccgttctgga
     8701 cgatgatgac gttcgacgcc gccccgatga tcgtcaggtt tccggcgatg gtggagccgg
     8761 cggcgagggc catcatcgcc tccaccgagg agtcgagggt ggagaggagg gggaggtaga
     8821 gggcgacgaa ggggacgttc gataccagct ggctggcgag ggcgctcgtc gcgaggatcg
     8881 ccgggaccga ggtcgcctca aaccccgagc cgttcatcgc cgcctggagg cggccggagt
     8941 tccagacgct ctccatcagg acgaacatgg aggcgaagaa gacgagggtc ctccagtcca
     9001 cattcttcac gagctcctgc ctccgggggc tggcgaggag gatggggagg gcggagagga
     9061 gggcgatctc cgtcaggccg aggggggacg ggaggcccga ggagacggag acgatcttga
     9121 aggcgatcag ggccgtcacc acccccaggg agagcttcga gaggcgggcg aggtccgggt
     9181 cgactacagc gtcctggatg tggttcaagg tctcctggcc aaactccctc ctgcagagga
     9241 gcctcagaac cccgtaggcg aggaggaggt ttacggccgt cgggagggcg aggtggcgga
     9301 agaaggtgac gaaggggttt gggacgctgc cgccgagggc gatgaggagg ttctgggggt
     9361 tgccgatggg gctggcgacg ctcccgatcg tcactccgaa ggcgagggat aggaggagga
     9421 gcctggggga gatcccgtgc ttattcgcca ggtggatcag aaggggggtt ccgatgatcg
     9481 ccagggtgtc gttcatcagc agggcggata ggaggccagc cccgaagagg accgcgatga
     9541 cgagctggtc gacgttcttc gtccgccgca ggagccggta tgagaggctc gcgaggtagc
     9601 cgctctcctc cagggccacc ccgacgacga acatcccgaa gaggaagagc atcacgtcca
     9661 tgtcgatcgc ccccagggcg gcggccgggg agatcgagcc cgcgacgagg acggcgacgg
     9721 cccccgccag catgatctgc cagatcatca ccctcgtcct gaggagctgc cggacggcga
     9781 tggcggcgag gaccgccgcc aggacgaaga tcgagaggtc tccgggagag agggggaaat
     9841 gagatgcgat ttctacttcg gacatgtagg ggtggctcgg gtcgcttcgg cccgaggggg
     9901 cggcccaggg gtcgcttcct ggacggatgg ggactatata cgcctttcca tcctcagacc
     9961 agggggttcg accccgagac cgcccgcccc gaagatcccg ccgccatccc tccggggaac
    10021 tccgggctcg cccgttaaac tataagccaa attaaaaaga tacaaagata tatgtataat
    10081 ccatcccctg gattctttcg aggaggagtt ttgaggcttc tccgaaggga gagatcgggt
    10141 ggaaggggtc ggcgacctgg aggctctggt gggatatttg agggagcatt cgccggcatc
    10201 cccgccccaa aaaaggagga tagccgcggt ccaggagagg gagaggctcc tcttcgcccc
    10261 ccgggccgct tccgcctgat cgatcggccg ggagcgagcc ctcccggcgg cgacctccag
    10321 gatctcctcc cgccctttca aaacctctgt cggggcgacc aaaacctcct tctatcccct
    10381 ccctcatcac caactggagc tttgattatg gcgatccatc ccatcgagta tcgttacgga
    10441 aacccggaga tgaaggatct ctggtcggag gagtacaggt tccgctgcct ctttcgcgtc
    10501 gaggcggccc tcgcccgggc cgaggaggag ctgggcttga tccctccggg ggcggccgac
    10561 gacatcggcc gggccgccga gggggccagc cccgaccggg cgaaggagat cgaggacgag
    10621 atcggccacg acatgatggc cgtcgtccac gccctcgccg aggcctgcga ggaggcgggc
    10681 gagtgggtcc acctcggcgc cacctccaac gacatcctgg acacggccac cggccttcag
    10741 atcaaggcct ccctcgccgt cctggaggag aaggtgaagc gggttctggg ggtgcttctc
    10801 aaacgggccg aggagcataa gggactcgtc tgcgccggga ggacccacgg ccagatcggg
    10861 gttcccacca cctacggtct ccggtttgcg gtctgggcct cggagatggc ccgccacctg
    10921 gagaggctag cccagatgaa gccccgggcc gcggtgggga agatgagcgg agccgtgggg
    10981 acccaggccg ccttcggtgc cgacgggatg aagatccagg cgagggtgat ggagctcctc
    11041 gacctttccg aggtggacgt ctcaaaccag gtcgtccaga gggaccgcca cgccgagatg
    11101 atttgctgga tggggctggt cgcctccacc ctggacaaga tctgcgttga gatcaggacc
    11161 ctccagaggt cggagatcgc cgaggtcgcg gaggtcttcg gcaagaggca ggtcggctcg
    11221 agcaccatgc cccacaagag aaaccccatc aagtcggagc aggtctgcgg cctcgcccgg
    11281 gtcgtccggg cccagatcga gcccgccttc gcgaacatcc ccctctggga cgagcgggac
    11341 ctcaccaact ccagctgcga gaggatcctc ttccccgagg cgttcatctt caccgaccac
    11401 atcctcgccc tcaccgcccg gatcctggag gggctatcga taaacgagga gcaggtcgag
    11461 agaaacctcc agcttctccg gggtctgaac atggcggagt cggtgatggt ggagctggca
    11521 aagaggggcg tcggcaggca gaaggcccac gagatcatga ggagctcctc catgatggcc
    11581 gtcgaggagg ggaggaccct cgcctcggtc ctggccgagg aggagacggt ggcggcccac
    11641 ctatcttccg aggagatcga ccggctcctc gacccccacc ggtacatcgg caccgccgtc
    11701 gagcaggtgg accggctcgt ctcgaagctg gcgcccctcc tgaggtgagg gtcctctccg
    11761 aggctcaaac tcggcagatg gtgaatttga tgggtgcgga ggtgacggca cagatcccgg
    11821 ccccgcctcg ggcggtcctc ctgaagccgg gccgcctcgt cagggacgag gatgggacga
    11881 tcctggacgc ccggtcctcg gcgaccctga tcctcgcagg cccgaagaag atcgtcgtcg
    11941 acaccggatg cgaaggcgag ggcgaggtga tcacgaatag gctgaaggag ctggggctcg
    12001 cccctgaata tgttgacatc gtcgtcaaca cccacgacca tccggaccac tccggaaata
    12061 accgcctctt ctccagagcc cggatcctct cggggaaggg cgggcttgag gagggggacc
    12121 tcatcgcccc cggcgtctgg atcatggaga cccccggcca caccgccgac agcatttccg
    12181 tcgtctgtga atctccccgg cggatcgtgg tggcggggga cgccctcccc ctgatgggaa
    12241 actatctgaa gtgggtcccc ccccgcctcc atgtggaccg agggatggcg ctgaggtcca
    12301 tggcgaggat cgtggaggtg gcggaggtgg tggtgcctgg ccacgattcg ccgttcctcg
    12361 ttcgagagag gaggtggacg gccgatctcg actgaggggc ctgagggcct ccccctttac
    12421 tgctaaaaat gtcatcctga tctctcttat atgtagcgac attcaaccca aaaagcgaaa
    12481 aaggcaatct attaatagta aaaaatgtag attggaggca agatgcctct gaaacttaca
    12541 tcaaaagaga agctggtcct ctacggcctt gcccggtacc ccggcctgag cgacatagat
    12601 ctcgccggca agatcggggt ggaccgatcg acgatcttca agagcaaaag gaagttcagg
    12661 gaatgggaga tggtccagct tctgaatgtc ccctccggga tgggggtggg ggcggagatg
    12721 atgaccgcgg tcttcacccg atacaggccc aacgcccccg tggaggtgag ggggaagaag
    12781 ctcgggttcg gtgagtgggt cgaccatccc cactgcgtct tccactccgc caccgactcc
    12841 gactcggtct ccatggtcta ctccaggagc tacaccgact tcaagagggt cttcaacccc
    12901 gtcatggacg actacaagga cgcgggcttc gtcgacgata tccggtgcta ccactatccc
    12961 ttctccctga cccgggtctc ctgggactcg gccctggcgg ttaacaacac cttcgagctg
    13021 gggaggaccg accttttgga cgagcctccc atcgaggagg aggcccccga ggaggaggtg
    13081 aagctcaccg agaaggacag gctggccctc ctcgccttcg tcaagtatcc tgccctctcg
    13141 gacctggagc tctcccgcag gactgggatc tctcggccca ccatatcggg gaagaggaac
    13201 aaattcttcc agagcgggct cctagcccgg caggcgaacg tcaactggaa gaagatcggg
    13261 tgcgagctga tgatcttcta ccacataaac gtccggccca aggcgacggc cgaggagatg
    13321 atgaggatct actcctcctt caggcggatc ggggcagcgg tatacagcta catccagccc
    13381 ggggagatct tcggctcctt cctctcccag tgctacgtgg agatgaagga gaggatggac
    13441 ctggaggtta aggccctcag cgaggcgggg ctggtggagg agaggcccag ctacgtcatc
    13501 atgcctctgc gggatctgaa ggttaggaag accgattacg cccccatggt gaagtctatg
    13561 ctggaagttg agacggacgt atgaatagag agatcgccgt catcgccacc accgccgact
    13621 ccgcaagcct gaacatcgcc gaccatctct tggggctagg atcctgggag gagggggggg
    13681 ccttccgccg gctccgaaac tttcgcctcg tcctcctgga ggagaggctg atcggcctcg
    13741 aggggctgga ggtgaggctg gaggggatgg gcctctcccc ggccctcata gtcttcgcct
    13801 cgaggcatca ggcgaaggat ggggcgcccc gcctctgcgg ccacttctca ggaaacccag
    13861 gagaggcggt cctgggaggg agggcgaggg agctggccgt cgccgccccc ggagccctca
    13921 agtccttcat atcgaacctc ctcgcccacc ccgtagaggg gtttgatgtg accctcgagg
    13981 cgacccacca cggccccacc gatttgaccg tgccatcctt cttcgcggag atcggaagct
    14041 gtgagcggga gtggtcggac cccgccgccg gcgaggcggt ggcgaggtcg atcctggacc
    14101 tagaggagct tcatcctccc gccttcctcg gattcggcgg cggccattac gtgacgaggc
    14161 agaaccggct cctcctggag acgaagatcg ccttcggaca cctcttctcg aagtaccagg
    14221 cgagagacct cgagatcgag atcctcgagg aggcgaggat gaagtcggag gcggtgggag
    14281 cttacctcga ccggaagtct ctccggtctg ccgagaggga gaagatatcc ggagccctgg
    14341 aggagatcgg ccttcaggct ttgagggagg gggagataag ggagaggtac cccctgcccc
    14401 tcctttcctc ttaatccagg gggagcctcc cggcctcctc cagcccaggc atcggaggtc
    14461 cggtggctga atctccccta taatccgatt cggcagccgc cttcctctcc tcgaacctcc
    14521 gacttttaga gatattcatt gaaataacta aagtttcctt gatctttggt caatcttttc
    14581 taataataca aaaatgttat atacccgata gaccccttta agctcgtccc aataagggta
    14641 ttcaccctga tagaggggga agatatggag agatcatcca aaacaaaagg agaatttttt
    14701 ccatgatggc ggcggtgctg gaggggccgg gagagatctc cctccgggag gtggacgacc
    14761 cggcctgccc cccggggggg atggtcctgg aggtcgaggc ctgctcgata tgcagcaccg
    14821 acgtaaaaat ggtgaggcgg gggcagcggg acctggtatg cccccggatc ctcggccacg
    14881 aggtctccgg ggtcgtcgcc gagagggacg gggccggccc cagggtcggc gagcgggtcc
    14941 agatataccc cggcgtatcc tgcgcaaact gcccctcctg caggagaggg gcggaaaacc
    15001 tctgcgggaa ggtccggatc ctcgggttca gcctcgacgg cgggtttgcg gagcggctcg
    15061 ccgtcccggc ggagtcggtg agggacgggg gcgtcttccc catccccgaa cccgccacct
    15121 tcgaagaggc ggccctagcc gagcccctcg cctgctccat caacgcccag gaccgggtgg
    15181 gggtatccga gggggactcg gttattatct tcggcgcagg ccccgccggg gtcctccacg
    15241 ccatcctggc ccaggaaagg ggtgcctcga tggtggcgat cgccgagcct ctggaggagc
    15301 gggcccgcat cgcgaagggc tgggcggatc aggtcctccg gccggggagg gaggatctcc
    15361 aggatggggc gatggaggcg accgagggga ggggctttga cgtcgcgatc ctggcgagcc
    15421 gggatcgccc cgtcgacggc tccctcctcg ccctcctcgc cccccggggc cggatctccc
    15481 tcttctcagg gctccctccg agtttcgagc ggccgaggct ggatctgaac ctgctccatt
    15541 accgggagca tctgatcgcc ggggcctacg gctgcacccg ggcccagaac ggcgaggccc
    15601 tggagctgat cacttccagg ggcgtggacg tcgggggcct attcaccaag agggttcccc
    15661 tcaggaagat atcggcgggc ctcgatcacg ccgaacggcg ccggggtctg aaggcggtgt
    15721 tgacggaatc tggaaggtga aggatattga aagactcgat ggaggagttt ggatctaagc
    15781 tgaaccggct gatagccggg gagagcctca ccagggagga ggcgaagggg atgttctctt
    15841 cggtgctgtc agacgagcag aacgacgccc atcagggggc cttccttgcg gcgatcaccg
    15901 ccaaggggcc ttcccccggg gagatcgctg gggggtggga ggcgatatac gacctcgaca
    15961 ccgtcaaggt cgatccccag gtctccgggg agctcttcga gaactgcggg acggggatgg
    16021 acggcgtcga taccttcaac atcagcaccg cggcttcgat cgtcgccgcc gccgccgggg
    16081 tccccatggc aaagcacggc gcgagggcga tcacctcccg ctgcgggacc atcgacatcc
    16141 tggaggcgct gggggtggag gtggaggcag gccccctcct ggtcaaaagg agcatcgaga
    16201 gggcggggat agggatcttt aacgggatga gccccctggt ccatcccctg ggcctgggcc
    16261 ggatcctctc caagatcagg ttcgggacgg tcctgaacat aaccggctcc ctcgctaacc
    16321 ccgccatgcc ccgcctgggg ctgaggggag tttactcaaa agagctggta gtccccacgg
    16381 cggaggcgat gaggatgatc ggctactcca gggcgatggt cgtctgcggc ctctccgccg
    16441 acggccggag ggggatggac gaggtatccc cctcggggag gaccctcgtc gcggagctcc
    16501 ggggagatgg caggatcgcg acctttgacc tgctgccccg agacttcggg gcggacccgg
    16561 tggacgaaag tgatctcctc cctcgtgaag atataacgga ggaggcgacg gagctccttc
    16621 gggtcctggc cggccgggag ggggggccga gggaggctgc catctgcatc aactccgccc
    16681 cgatcctcct cctatccggg aaggccgaga gcctcccgga ggggtattcg atctcaaagg
    16741 ggatcctccg gtcgggagat gccctgaaga agctcgggga gtgggtctcc gcccagaact
    16801 ccgaccccat cgccggggcc gagatcctca acgccctcct ccatcgggta ggaaattgaa
    16861 ggccgggaag aagagggatg gggggatcaa ccctcccccg cctcgatctc gaggatcccc
    16921 gagaccttcg ctaggtaccc cttcatgtag gcgtcgcaga cctcccactc cacgtcgacc
    16981 tcgacctccg ccatgatctc ctggagctgg cccttcacct gctcccaggt gtacttgttc
    17041 aaagatctgc atagctccgt caggaggcga agagtgggga tgaagatctt cttgaactcg
    17101 gcccggatga agcccatgaa cggctcggag aggtcccggt agacgtagga ccggagctcc
    17161 gccaggagct cgtactgggc ggtgcaggga tcgtcgtaag gatcggggga ctctctctcc
    17221 cagtgctccc ccagcttctg gtaacccttc ttgatcatcg cgtcgaccct cgccctccct
    17281 tcgccgtccc cgatctccgc cgcgagcttg tagcccgtca gccggagccg gaggagctga
    17341 accctcccct cctcaaaggt gaggttgagg tgaaacctgc ccaggtactc ctcgatgtgg
    17401 tcgagcctcg tcgacgggtc gaaggttatg gccaccgcat cacccctccc ccgaggaggc
    17461 gtaggaggcc cccgtccaaa ggtccaggat catatccggc ggcctcggac catcgccagc
    17521 cgcccgaccg aaggttcgtg acccattcat atttggagtt atatatcttc gcagtttaat
    17581 acctttggca tcggatggga cgtccccagt tctttgagcc cgcctcctcg ggggcataaa
    17641 tttatccgaa gacggatcgt aagccctggt cgtcaggagg gtttggatga agatggtgcg
    17701 ggtctttctg gagatcatga ggccgggaaa ctgcatgatg gcggggatcg ccgccctgat
    17761 agggctgatg gtggcggata gcggccctct ccccgttacg gcggctctgg tattcatcgc
    17821 cgtcttcgcg gtgacggggg cgggaaacgc cgtcaacgac tacttcgacc gggagatcga
    17881 cgccgtcaac cgccccggcc ggccgatacc gagcaaaagg atcagccctg aaagggcctt
    17941 tgcctggtcg atcctcctct tcctcctcgg gtcgggggtc gccctcctca taaacgcctt
    18001 cgccttcgcc atagccgtcg cgaactcgat cctcctctac ctttacgccc ggaacctgaa
    18061 ggtgacgccc ttcttcggaa acctcgccgt cgggtacctc acgggatcga ccttcctctt
    18121 cggtggggcc gccggcgggg acgtcgggat caccctcttc ctcttcctcc tcgcgaccct
    18181 ggcgaccctc gcccgggagg tcgagaagga catagaggac gtccccgggg atcgagccag
    18241 cggcgcccgg accctcccga tcgtcatcgg agagcggcgg gcgtctcacc tcgccgcctc
    18301 cttcgtcctc atcgcgatcc tcctcagcta cctcgccccc ctggggaggg c
//
LOCUS       DASM01000044           12302 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_203056, whole genome shotgun sequence.
ACCESSION   DASM01000044 DASM01000000
VERSION     DASM01000044.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12302)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12302)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12302
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_203056"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    999..1008
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2565 a         3587 c         3654 g         2486 t
ORIGIN      
        1 gcttctctcc cccgctcagc tggtgagggt aggatcgacc ctttgcctcg tccagcccca
       61 cggccctgaa gaggcggctc acccggccct cgacctctcc gggatcggcc ctctcccgat
      121 ggacatatat cgcctccccc acctggtcgg agacggtcat gatggggttt aaggcgtcct
      181 cgacgttctg gaagaccatg gccatctcct tgccccggat cctccggcgg gctacctcgt
      241 cgagggggac gagatccttc ccgtccagga ggatcctccc cgagaccttc ccctccaaaa
      301 ggcccatgag ggcgagcccc agggtcgtct ttcccgcccc ggactcgccg atgatggcga
      361 gggagtcgcc ccggaggagg tcgaggtcga cccccgccag gatcagatca ccgtcgtaag
      421 agaccttcaa atcttggatc ttgagcatcg tcatatacct ccatcatgat ggtcgtcccg
      481 atcccggagg cgcgggtcga gggccgactc cagggcgtac cccgtgaagc tcaggccgat
      541 gagggtggcg gagagggcga tccccgtcgg cagaagccac cacttccaga cctccaggaa
      601 ggtgaagacc ctggcatact ggatcatctt cccccagctc atcaccgagg ggtcggagat
      661 ccccaggaag gatagccctg cctccatgaa gaccgccctc ctggcatcct ggatcattat
      721 ggccaccagc accggcgcca ggtccgggac gatgtggcgg aagaggaggt gaggccagtc
      781 ggcgccgaag gtcctggcgg cggagacgtg catcctctcc ttcagggtga gggtctgggc
      841 ccggatcacc ctcgcccctc cgggccagga gagggcggag aggaggagga tcatgaagag
      901 gacgttaggc cggaggtacg ccgccaccag aatgatgacg atcacgtcgg ggatggcgat
      961 ggtggcgtcg acgaccctca tccagaaccg gtcgtagann nnnnnnnngt cctggcgccg
     1021 tagaggagct gggtccagat gtcctggcgg gcgtcgttcg tccccagcca gtgatcggcc
     1081 gatgggcggc tgaaggctgg ggtgctggtg ttctcccggg gggagtaggg ggtgaggagg
     1141 ggggcgaaga tggcgaggag gatcatcaag gcgatcagaa ggagccccat cctcccgacg
     1201 ttgctcccca gaacctcccg ccagacgtca acgtgcatcg ctcaccctcg gatcgagctt
     1261 tgagtagagg aggtccacca ggaggttcgc cgccaggacc gccaccgtca ccatcagaag
     1321 gatcccctgg atcagggggt agtcccgggt caggagggcg ttgtagagga gccaaccgac
     1381 cccggggtag gagaatacga tctcgacgaa gagggcccct gtgatcatgt gggggatcat
     1441 catccccgtg gaggtgacga cggggaggag ggagtttctg cccgcgtgcc ggtacttgat
     1501 ccttccttct gagcagcctt tggccctggc cgtcatgacg aagctctccc ggagggtggt
     1561 gatcatggtg ttcctggtga ggaggtaagg ggaggtcaac ctcaccagga ccagggcggc
     1621 gaggggggcc atcagatggt ggaggatgtc cagggccagg ggcagccccg ccaggccgct
     1681 gtaaggggtg agggcgccgg agagggggac gacccccatc aacaccccga agaggaggag
     1741 gaggacgatc cccatgaaga agtcgggaaa gccgttgagg aacatcaggc ccccgaggag
     1801 ggccttgtcg aggcggcgac cacggcggta gccggactcg atccccagga cgagcccccc
     1861 cagggtggat aggaggagcg ccgtccccgt cagcagaagg gtccagggga ggaagtcgag
     1921 gatcaccgtc ataacctgct ggttgtagta gtaggagtag ccgaggtctc ccctcaacag
     1981 ggcgaggagg taggcgacga actgcgagcc catcgactgg tcgagggaga gccgctcgat
     2041 cagctgggcc ttcagctggg gggtcatggc gaggagggcc tcctggccgt agatcgcctg
     2101 gaaggggtct ccgggcatca acctcggcag gacgaagttg agggtcagga tcaggaggaa
     2161 ggctacggcg tagttggtga agaaggcggc ccgccggagg agggcccaag ctcgcgccgt
     2221 cgccttcggg atactccatc cgaccttcgg cccctccaga gggcgcccat actggtcctc
     2281 catcgaacga tccgcctccg tcgcctcatt ccggccttct taataaactc tccttattat
     2341 agcactcaaa ttctcaaggg tttatctaat cgggtcatta tttatgaaac tttcccggat
     2401 cgtatgtaaa tcaaacaatc gatatgatga tctataacac agaatctgct ccaatttaac
     2461 aattgagcct ggaagaaaga gggaacgatg gggaaggtca ggcggccccg tcgttcaagg
     2521 tggcaaattc gggggcgctc ccctcctccc ccttccgccc ctccgccggt cttccgccgc
     2581 cgggcaccac ccccctgggg ggtcgccccc gagggtcagg ggcgagaggt gggggaaggt
     2641 cctgcagatc atcggccggg ccccatggat cttgcagagc ccttcggcct cgtcgaggaa
     2701 gccgcacggg agggtcttct tcatggccca ggcctcatac ccccccacct ccacccggtg
     2761 ggcgtagatc tcgaccgtct cccttatgcc gatcccgaga tgggccgaga gcctctcgac
     2821 gtcctggggc tccagggtta tgggggtgga gagggtgcag caaagcccgc acatcctgca
     2881 ttcgaggtgg gagtgagcct cgaagagccg ggccccaagg tccgggtcgg ggactccggg
     2941 atagaggccg accgccccca tcagccggac cgcctccatc acccgtcgat ggaccggggg
     3001 agcgttgaag gccgccggga gccaggtggg ctgatcacag ggcctctgga cgtaagaaac
     3061 cggcaggtcc agaaagaggc cgtcgaggtg gtaggcggtc ctggagatcg gggaccagag
     3121 gatcctcccc tcggccccca gggagatgag ggcctcggcc acctcccggg ataggccggg
     3181 ggccaccacc aggttctcgg cgcaggtgac cgccccttcc gggtcctcgc cgaaggcttt
     3241 agctatcctg gccgaggtca catgcctatc tgagctgacg aacctctcga tcctccgctt
     3301 caacaaccta tccgccatct tggagatcgc ccccgggtaa tgccctcccg tgggaggagg
     3361 tcctctaagt aggtatccct gaagatcccg gcttcgaaaa aagggggggg tatgccccgg
     3421 aggggggcat cgttcccgac gcctagagga aggccatctt gttcaagggt atcggaaccc
     3481 cgatggagat cccgccccgg gtgaagtaga gggcgacctc cccgttgtgg gcccagtacc
     3541 agcgggggta gtagagggtg agggcgggga gggcctcggc gtagaggacc tgagcctcgg
     3601 cgatcatctc ggcccggtcg tccccggcgg tctcggtcct ctgatcctcc aggagggccg
     3661 tcaatcggtc gtcctcccgg taccggacgc tgttgagggt ctcgtccagg gtcatcctcc
     3721 ggaggtagtc ggggtcggcg ccgaggccgc cgtggccgag gagggcgagg tcgaagtccc
     3781 aggccatcac caaggtgtcg agggtggctc cgtcgaggga ccggagggtc acctcaagcc
     3841 ccgcctcctc cagctgctct tcgatcagct ccgctgcccg agagccgggc cagccgacga
     3901 tccccctctc cgccaccagg acctccacct cagtgccaga gtggccgaga tcctcgatca
     3961 tacccctcgc cagctccggg tcgtattcat acgcctcgac ggcggggttg taccagtcgc
     4021 tgtctggagg caccatcccg gggctcccag caaggccctc gccgcggagg acgatctcga
     4081 ccagggcctc gcggtctatg gcgtgggcga gggcccgcct gaacttgggg tcggagaagg
     4141 gctccttctg gtggttgatc atcagcttcg cgttccagtc gaaagccccc tcgatgatct
     4201 caaggccagc cccctcgagg gaggagaccg cctccggggg gacggaggcg gcggcggcgg
     4261 acccctgcct cagggcggcg ggggcggtct ggtcggagat cttgacgaag aggatccgcc
     4321 cgaccttcgg ggcgcccata taatagccgt cgtaagcctc gaagaggtag gtcccctggg
     4381 ccttattgta gtccgagagc ctgaaggggc cggtccccac cagggcctca tcgtccctcg
     4441 cgccctcggg gtcggagacc ccctcccaga tgtgctgggg taggatgggg agggtgcagg
     4501 cgacgaggtc catgaagggg gcgtaaggct cggtcaagta gagcttgacg gtgtaatcgc
     4561 cgcccgcctc cgccgactcg atgatgctgg agtcgaccca ctggtagggg tggtcctgga
     4621 tgtactcgac ggtgaagacg acgtcttcag cggtgaacgg ttcgccgtca tgccaggtga
     4681 ccccctcctg aagctcgaag gagtaggcgt tctccgcctc gaggtactcc cagccctcgg
     4741 cgagggccgg aaggtacccc tcatcgtcct tccagaccag ggtgtcgaag atgaagctca
     4801 tcctgacgta tcccggaccc cgggagtagt gggcgtaggg ggagggaaag ccccagtccc
     4861 ccgtcgcatc ggcgatggtg tactcggcca cctcgtatcc ggcgactccg gcggggccga
     4921 tagccaagag cgccaggaga gcgagggcca gcccagagat caagatccac ggttttagca
     4981 ctttgaaaca cctcttcctg aatagtcgaa tctgatgtta tgtagatggg gtctattcgg
     5041 cttatttatg aacttattcg ttttagaaat gtccgttata gtatctaaat gtcaattttt
     5101 acagatataa atatcaaatt gtgttgctca ttagatatat caggatcaaa tcaaggccaa
     5161 tactggaatt atttgcccaa taacagctct gatgggtctc ctatcgtatc ataagacctt
     5221 ttagtcatac taataacagt aaacctctta aacccggagg tgaaaccgga cttaaagccg
     5281 gagatggaac cgtccacaat ccgccaatcg cccccgcccc acccccgccc cgacccggcg
     5341 acggctcgcc catctgctgg ttgattgctt atctgctatt tccgtgacgg gggcctctga
     5401 ggtgtaattg accatgaacc cgatgaccga gatcgactgg aacgaggtat ggattgacca
     5461 gatgcggcga agccgggagg cgagcaccgc ccgggactgc gcccggatct gggagagccg
     5521 ggagagcgcc ctcaagttct ggaatatgtc taaagagaac agccaccgcg tcgaggagac
     5581 gatcgcgggg acggagatca cccccgactc cagggtcctg gacatcgggg ccggccccgg
     5641 aaccctcgcc atccccttcg cccgaaaagt cgccagcgtc accgccgtcg agcctgccga
     5701 ggggatggtc tcggtcctcc aagagaagat ggccgaggtg ggggtcagta acatcgacgt
     5761 cgtccagaag cggtgggagg acgtctccgt cgccgaggac ctggacccgc cctacgacgt
     5821 ggtgatcgcc tccttctccc tggggatggc cgacatccgg ggggcgatcg agaagatgat
     5881 cgcttccgcc gcaaagggcg gcaccatcta cctctaccac ttcgccggag agacccactg
     5941 ggaccgggac tgccgggacc tctggccgag gctccacggt agggagtacc agcccggccc
     6001 caaggccgac gtcctctaca acgtcctcta ttcgatgggg atctacccct ccatgagcac
     6061 cttccggctc cagcacagcc agcgcttcgc caacaaggag gaggcgatgg agcacttccg
     6121 ccagcagtac cggataacga cggcggagca ggaggcgatc gtgcgggatc acctggggcg
     6181 ggttctcgta gaggagaacg ggggcttcag cctcccggcg tcgtcgatca gggtgaagat
     6241 ctggtgggag acggggtcgg aagaggatgg gttggccgaa aggtgacgct acggcctcct
     6301 agttggggga agggctgggg ctcgttcggg ccagtccatc gcctttggcc ctggggcaat
     6361 ccagtgaagc tgtgctttgt cggctggtga taggtgagga ctatgcgcat ctgtttagaa
     6421 attatggcac tgtcataaag gcgtgtgaaa tggcctctat actattaccg agatgatttt
     6481 taatcctcag tcagtcctaa atctatatta atatgcgcaa atttcaggga taagtaattg
     6541 attagcggag ggcttatatg aataatgtcc atatccaatt agttttgact aaggtcgaaa
     6601 aatgttattt aggttttaga tctttcattt ttccattcca cgtgccagta tgatatccaa
     6661 ttctccattg tccagtaata tctaatatac ggaattcccc aatgtcttcc catgttccat
     6721 tcaaccatag ttttcctcga gaacttacta acgtatacca ataaacgata attgttaatg
     6781 caattataaa cagggcaata ataataattg ttatcgcctc aaagttagcc ataaaatgac
     6841 ctaacaatga aattaaagta attatagtaa tgctgatgat gataaccaaa tgagcgaaat
     6901 acctaagaaa ttctatattt gcttcgagtt gcccttcccc tttcatggtt ccctttccat
     6961 tgcctgtcac ctcccatttt ccttctgcgt tccattttcc taatagcaat cctcctgccc
     7021 acgaccctat tccaatccat cttttatctt ctagatacca ttttccaata ccatcccatc
     7081 tccctgacat agctttacca gaccatattc tactctgcca tattttgtct tcttttatta
     7141 tcgcttcgtc aattaatttg gcggcatatt ttaattttcc catatttgaa ggttgattta
     7201 atccctctat aatttgcact ctatcaggtt catccatatt gtttgctaaa tattctataa
     7261 aatctcgcaa ctttttttgg tcatcgacac tcattttatt atagatagat tctaaattaa
     7321 atgatgcgct ctctaatctt acatagaaat tatttaaata ttcctcaatt aaatcggatt
     7381 ttattttaga cgtatctaaa taattctccc attcttttaa tattgactct ttgagtttcc
     7441 tttcggcttt taacttattt tggactctta ttacactagt aatcactaaa aaaaagcttg
     7501 aaattgcgat aattatgccg actaaatcca aatatgtcat caagacgaaa ccaccttctc
     7561 ttcttctata aatgggggga atggcgtcct atcaatcaca tggaggttat atctttctac
     7621 accatgtgca aaaaaaacaa atgctataga aatgcgggca aataaatcaa aagtgcctga
     7681 actcgttgat ccttctgaaa tagccccaaa tgcataagca aaaaatatgc atattattaa
     7741 tgtaagtcca aagaaaagct tacttctaga tgacactcca atagcaatgg aatacattga
     7801 agcagctaag gttattgatt ttatacttac ggcactagta gaacaatatt caaaaatgat
     7861 tggtaaaaga ggcaggccca tatgcaagag gatacataaa ccgaaactct cccaatattt
     7921 cggtattgcg ggaatcctat gtgccaattt acaccttcct ccttattctg cattaatcaa
     7981 gtacgcacat cggatttaat tctttttctt actatatgat attatgtagc taaggtaaac
     8041 ttgttggagg caattacgga tatacgaata cgttaaacat aaagaaaaag tcaaactggt
     8101 agcggcactc tgtgcagcat taagcgttca gtccgtgtcc accagccacg cctcgccctc
     8161 gggttcaaag ccttcaacct cctcgaagaa cccagcgatt ccggccggat gattttgctc
     8221 ttcaaggagc gatcgatagc ctgggcacgg ggcaaatgcc ggattgccgg acgcaaggcc
     8281 cccaaattcc aaacttgccc attttctctt tctctcagta gtggagcagc gtcccttcca
     8341 tcttcgcctt ccggaccgca tacctgaagg cgagaaggct cgccggcagg agcatcgctg
     8401 agaagaggac cagggtcccc acgtccggcg ccagctcccg caagctgtag ccctggagga
     8461 gggcgtgcct catcccgttc agggagtagg tcatcggcag aaggtgcgag accgtctgga
     8521 gccaggtggg gaggacggtt atggggaaga agaccccccc gaagagctcc gaggtgctca
     8581 tgaagagcat gttgatgggg tcgcccctct tgaagatcat gatgaagctc gccgagagga
     8641 tcccgatgct ggaaaagcat acgatcgtga ggccgaggat gatcagcgcc ccgaagacgt
     8701 tggcgccggc gaggctgacc cccaggagga ccccgatgac gaggtatagg aggacccgga
     8761 aggaagcgaa gatgaagttc cagagggtcg atagggtgat gatcgccgag gcgtctgttg
     8821 gggtcaccag catcgcctcc agggtcccca tcatctgctc gtcccggacg ctctcggaga
     8881 aaccccggag cgagaccatc aggtaccccg agaaggcgat cccgatgagg acgaaggaga
     8941 agtagtcgcc gccgtaaggc tcgaggtact tcatcccggc ggtcccgatg agcttggcga
     9001 cgaagtagta ggtgaggatg ttgaagaaca tgaagaagag ccggaggaag aactcgaact
     9061 tgtagctcat ctggaggatg tagctcctct ttacgaagga gagggccttc cgataggtcg
     9121 cgaacatact acccaccttc ataccccgcc cctcctccgg ccgaggccgc cgccccggtg
     9181 gggaaagccc cgccggtccg ggcggaagcc cggtgccgtt gccccggcct cagtcccgcc
     9241 ccgctcctca aacctctttc cagtatagtg gaggaagacg tcggatagct tcgagccctc
     9301 gccgagggca gccttcagct cgttcgggga gccgattacc tttatcttcc cttcgtcgaa
     9361 gatcgcgacc ctgtcgcata gctcctccgc ctcctccagg ttgtgggtcg atacgaagat
     9421 cgtctttccc cgctcccgga cgatctcttc cttgatgaaa tcccggaggc cctgggcggc
     9481 gccggggtcg aggctcctcg tcggctcgtc catgaagagg acggcggggt tgttgaggag
     9541 cccccgggcg atggccatcc tctgcttcat ccccgcggag tagttgagaa accggacgtc
     9601 ggcagcgtcc ttcaggtcca cgaccccgag gaggtcatcg accatatctc tggccttgtc
     9661 ggagtagaag ttgtgaaggg aggcgaagaa ctccaggttc tccctccccg tgagcctcca
     9721 gtagaaggac cgctcgtcgg tggtgaccag gcctatcgac tcccggaccc gtccgctctc
     9781 cctctccagg tcgaagccgt tgactttggc tctgccggcc gtggggagga tcagggtgca
     9841 gaggatcttg atcagggtcg tcttccccgc cccattgggg ccgaggatcc cgaagacctc
     9901 tcccttcctg atcaataggt cgaccccgtc gacgggggtg accccgccgg cggagggccg
     9961 ccgtcggagg aggtcggcaa ggcccctagg cgcagcgaag gtcttggtca gcttgcaggt
    10021 ctcaatcgcc gcctctccga tgatcccgcc tccagaagat ctccgcataa agaggcggga
    10081 tcacgacagc ctgatctcct tcatctcggc gagggtgcgg ggaagtctgg acctcctctt
    10141 cccggacgta aagaacctcc ccccagggtg cggccgcggg ccgttcggct tgatctgctt
    10201 tatggggacg atgaagggct cctccgactg ggaccggacc gccacctcga cgttgtagat
    10261 cgcctcgatg ttctcggggg tgaggacggt ggcggggtct cccgcggcca tgatcctccc
    10321 cctcttcatc atgatgatcc cgtcggagta cctcgaggcg aggttgaggt cgtggagggc
    10381 catcaggacg gtcatccgcc tcttcttcac gaggtcgctc actatgttca tgacgtcgag
    10441 ctggtgccag atgtccaggt tgctcgtcgg ctcgtcgagg agcatcacct tcgtctcctg
    10501 gacgagggcc cttgcgatga ggaccttctg ctgctggccg ccgctcagct cggcgaagcc
    10561 gctcatggcc agctcctcga tcccgaggag cctcaagacc tcccagaccc tctcctcgtc
    10621 gtcggagctg gaaagccagc cgatgtgggg cctcctcccc atcaggacgg tgtcgaagac
    10681 gttggaggag aaggtccggg ccgagctctg ggggacgtag gagaggtttt tggcgatctc
    10741 catccgatcc atcttcgtca cctcctttct atcgatgagg atgctgccct tctccggggc
    10801 gatgatccgg tcgatgcact tgatgagggt cgactttccg gagccgttgg gccccactat
    10861 cgtcacaagc ctcgacggcc cgatctcaaa gctgacgtct ttgaggatct gggagctgtt
    10921 gtaaccaaat actattccct ttgcctgaag ctggatcatc atgtccaaaa ctccctcctt
    10981 ctccctttca acataagata catgaagaac ggaactccta tcaccgatgt catgatcccc
    11041 gtcggtataa ccgtcggggc gatgaggttc atagcgacgc cgtcggcggc gatgaggacc
    11101 gaggccccca ggatccccga ggcggggatc aagaaccgat gatctccgcc taggatcagc
    11161 cgggccatgt ggggtgccac cagccctatg aagccgatcg tcccggtgaa gcagacgacc
    11221 gtcgccacca ccaggctcga tatcagcatt atgtaaaccc tgacccgctc ggcgttgacg
    11281 ccgatgctct tggcggtctc gtcccccgag gtcatgatgt tcagatccca ggccttcagc
    11341 atcagaaggg ggatgcagat cgcaaagacc ccgaggagga ttgagatctt gctccaggag
    11401 aaggcggcga ggtcccccat cccccaggag gtcatcagcc ggagctgctc gtcatttgcg
    11461 aagtacttga agagctggct catggcggag aagaagtagt tgacggcgat ccccgccagg
    11521 atcagggtct ccgaggtggc gcccttgacg gaggcgaggc ctatgatgaa ggcggagcag
    11581 atgagggcga ataggaaggc gtttccaatg agcaggtacg ggccaccgaa gaccgatatc
    11641 cccaggaccg ccgccacggc tatcccgaac tgggcccccg acgagatccc cagggtgaag
    11701 gggctggcga gggggttctt caggaccgcc tgcatgacgc acccgcatac cccgaggccg
    11761 aagccggcga ggatcccgcc tacgatcctc ggaaacctta tgttccagac aaccctctcc
    11821 gtcagggggt ccacctggaa tgtgccggga aaagccctcg acaagagggc gcggtaggct
    11881 tcggcgacgg agatgtccag ggggccgagg gttatgatga accccgccag aaggacgatg
    11941 agggcgacga tcgccgccat gaagaagtac cgtgcgaata cgaaggagtc gtgggcctcc
    12001 tgcatcttgc tcctctttcc cgaccgggtc gaggaccgcc tcaccgtctt cgccatatcc
    12061 acacccgggc cttacttctc aacgacggcc ttcgcaaggg ctgcggtcgc acagatatcc
    12121 tcggcagagc gcctggggaa ttcggagccg gtcgatacca cgatgaggtt cttctctttt
    12181 gcgatctcgg tgagggtctg atactccctc agcttcatgg ccgagggctt ctcctcgtag
    12241 agggtggcag catcgttcat cctctgagag gcctggtact ccccctcggc gaggatgatc
    12301 ct
//
LOCUS       DASM01000045           15594 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_3346, whole genome shotgun sequence.
ACCESSION   DASM01000045 DASM01000000
VERSION     DASM01000045.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 15594)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15594)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15594
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_3346"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2702 a         5114 c         4513 g         3265 t
ORIGIN      
        1 cgccttcaaa agcctgatgt tcccgcttcc atcggtggcg tagaactcgc cctggcctgc
       61 ggagggatcg tagaatagga ggtcggtgaa tccgtctccg ttgaaatctc cggggacgat
      121 gatcgaccat gtcctccgcc agccagagct gctcttcaac agctggatgt taccgctacc
      181 gtccgtcgaa tagaactcac cctggccggc ggccggatcg tagaatagaa ggtctgcata
      241 gtcgccgccc gtaggcccag ggaagccgtc gctttcatca tgctccggcc ccggggagcc
      301 gtcagcgccg ccgctgaaca ctccggggac gatcgatgac catgtcctcc tccatccgct
      361 gtgagactgc aggagcctga tgttcccgct cccatcggtg gcgtaaaact cgccctggcc
      421 ggcggcggga tcgtagaata ggaggtcggt gtatccgtcg ctgttgaagt ctccgggcat
      481 gatcatcgac catgtcctcc tccatccgct atgagattgg aggagcctga tgttcccggt
      541 cccgtcggtg gcgtagaact ccccctggcc tgcggccggg tcgtagaata ggagatcggt
      601 gtactcgtca ccgttgaagt ctccggggac gatcgatgac catgtcctcc tccatccgtc
      661 gctcgccttc aaaagcctga tgttcccgct cccatcggtg gcgtagaact ccccctggcc
      721 tgcggccgga tcgtagaata ggaggtcggt gaatctgtca ccattgaagt ctccggggac
      781 gatcgatgac catgtccttc gccacccgct gtgagattgg aggagcctga tgttcccggt
      841 cccgtcggtg gcgtagaact ctccctggcc ggcggcgggg tcgtagaata ggaggtcggt
      901 gaacccgtca ccgttgaagt ctccggggac gatcatcgac catgtcctcc tccatccgct
      961 gtgagattgg aggagcctga tgttgcccct tccgtcggtg ctgtagaact cgccctggcc
     1021 tgcggccgga tcgtagaata ggaggtcggt gaatccgtca ccattgaagt ctccgggaac
     1081 gatcatcgac cacgtcgctc tccagtttcg ataatttttt aagagatctg cgtttccggc
     1141 cccgtccgtc gaatagaact caccctggcc agtggtggga tcgtagaaaa gaagatccga
     1201 ctcacagaaa gctccgtcca ggcagaacgc cgagatcatc aagaccatcg ccagtccctt
     1261 ccagctcatc tcttatcacc tcagatcctc tcgattattc atatcaatag aaataaaata
     1321 cgatggccag atataaatag cttcgtctcc ggtcgatttt tcgaatgagt gaaggggctc
     1381 caccccctcg tcgacgacgg cccctgtctc cgggctctcc ggttttgctc ctccgaccaa
     1441 taggtcccat ctcgggcttg tatccgcctc ccaagttccg ccacctttta gtagaaataa
     1501 ctaactaatt aatcgatata atctgcaagc tctcgattaa gatatcagga gaacgaaata
     1561 ttatgagaac cgatatcaaa tctgctggca aaggggtccg gggaaggtct ctggccctcc
     1621 tcccgaccct tccccttctt ctgacggtca tcctcatagg aaccgctttt gcccttccct
     1681 tctcggtagg gccgaatgat aaagacgagt accatttcct cgtcggcgcc cccggcgaga
     1741 tccgggttca ggcctcctgg acggggaccg ccaataacct cgccctgatc ctcaacggcc
     1801 ccggccaggt gaacgcctac gcccgacagg acggcccaag ccccctggag atccgctatc
     1861 tcgtcgccag cgccgacgtc gccaaaggcg aaacctggaa gatgggtatc tggaactttg
     1921 gcggagggac ggcggacggg gacctgatcc tcacctcccc gggcgccccc ttctctgtcg
     1981 gctccggatc ggcggaagca cttgagatcc gcttcccggt gaggtctccg gggctgatca
     2041 gcgcccaggc catctgggct agctccgcag atctcgacct cgccctcaac ggccctggaa
     2101 atttcggcac tcatgccctg ggccagagcc ccctggggaa ggggtacatg gtcaccaacg
     2161 acgacctcct gaaggggcag acctggctcc tcaccctcga gagccacgag cttgacccgt
     2221 cccagaccgc caccgggacg atccatctct cttatcccgg cgagtgctcc tggaccggga
     2281 cctgggagac gaacttcggc gatatggagc tggtccagga ggggtccacg gtcaccggga
     2341 gctacaccca cgaccagggg cggattagcg ggacggtctc cggcaacacc ttcacaggga
     2401 cctggtcgga ggccccctcc tacagtccat cgaacgatgc cggaggggtt cagctggtaa
     2461 tggcggaggg ctgcacctcc ttcaggggct cctggaggta cggccacgat aagacgagct
     2521 gggacgggga ctggaccggc accagggcgc gagggggtct cgacctccgc cccaagggct
     2581 tcgggatctt cggatagggg ttttggggcg cctcagcccc cccacctccg ccagcgcctc
     2641 cggagtcctt tcaggctcca aagccttaac tcaccctctc ccatacccca ggccaggaat
     2701 gactgaagac ccttcccaga agatggcggc cctggcagcg gcggccggca gctcctttct
     2761 cacccccttc atgggctcct cggtgaacat cgccctcccg gcgatcggcg aggagttctc
     2821 cttggacgct gtcaccttga gctgggtggc gacatcggcg gtcctggcgg cggcgatctt
     2881 cctcgtcccc ttcggccgcc tcgccgacat ccacggccga aaaagggtct tcacctgggg
     2941 cgtcgtcacc tatacgggct cggccctcct ctgcgccctc gcgacctcgg ccccgatgct
     3001 gatagccttt cggttcaccc aagggatggg gggggcgatg atcttcggga ccggcaccgc
     3061 catcctcacc tccgccttcc ccgcccggga gcgggggaag gccctggggg tgagcgtcgc
     3121 cgccgtctac ctcggcctca ccctggggcc gtccctgggg gggctcctca ccttcacctt
     3181 cggctggagg gcggtcttcc tctctaacct ccccgtcggc ctcctgctgc tcctcttgat
     3241 cgcctggcgg ctccgagggg aggtggccga ggcccggggc gagagcttcg acctcccagg
     3301 ctctgccctc tactgcctcg gccttctcgc gacgatctac ggcttctcca ggctgccgga
     3361 gcccgttggc ggagggctcc tcctcctcgg cctcgccggc cttgctgcct tcggcctctg
     3421 ggaggcgagg tcggagagcc ccctcctgga gacgagcctc tttcgggaga acagggcctt
     3481 cgccctctca aacctcgccg ccctgatcaa ctacaccgcc actttcgggg tgggtttcct
     3541 cctcagcctc cacctccagt atgttctagg gcttaccgcc caggaggcgg ggctggtcct
     3601 gatggcccag cccgtgctga tgatcttcgt cgccctcctc tcgggctggc tcagcgacag
     3661 gatgagcccc gggatcctgg cggggctcgg gatggggctg acgaccgcaa gcctcctcac
     3721 cctcctcgac ctcggccggg agacctcctt atgttcggtg atcggaagcc tgatgatcct
     3781 cggcctcggc gtcggcctct tctcctcccc caacaccaac gccgtcatga gctccgtccc
     3841 ggcgcggctc tacggcgtcg cctcctcgac cctggggacg atgaggctct ctggccacat
     3901 gctcagcatg gggatagtga tcctcgccct ctccctttac atgggtcggg tgccgatcgt
     3961 ccccgagagc atagacctct tcatattgag cggtcaggca gccatcttcg ccttcgccgc
     4021 cctctccttt tttgggatct tcgcgagcat ggcgggctta cggcgaccgg agcctcccgg
     4081 ccggggtaga tgaagggcaa aacgggtgga tgcggatcga aattccctcc ccggcttcga
     4141 ggatcgacct catcatttat gtaaatctat accggctatt tttctacaaa atccgcgcaa
     4201 ccttgaaata gatggtggca tatatccggg gtacaggagt tataacaata ccatctgcga
     4261 tttctgttga gatcgcagca ggtcgccgaa tgtgatgaga atatcagaaa gtatctatga
     4321 ggaagtattg atatgaaaat ttgtgttaca gccaccggcc caggcctcga agcccccgcc
     4381 gacccgaggt ttggaaggtg cgcctacttc gtgatcgtgg acgttgatac gatggtggcg
     4441 gaatcgatct ccaactccag cgccagcgcc gccagcggcg ccggaatcca ggctgcccag
     4501 acgatcgcca acgcaggtgc ttcggctctt ataactggca acatcggccc caacgccgct
     4561 gagacgctga aggcggcagg ggtgaaggtc tacctcgcct ccggcgggac agttcgagag
     4621 gcggtggaga ggttcaagaa cggagagctc accgagacct cctccgcctc ggtccagccc
     4681 cattcgggga tgggccgcgg cggaggccgg ggcggcggtc gtggcggcgg aaggggacag
     4741 ggcggtcaga accggtgatc tggtatgaaa gttgcagttc caaccatggg ggataaaggg
     4801 ctcgaggagc tgatctgcgc tcacttcggg cgggccgaga ccttcaccat agtcgacatc
     4861 gattccgggg acgtcgaggt tttgaagaat acaagcgagc acatgggagg gaccggcctt
     4921 cctccggagc tgatctccgc caggggagcc cacgtcatgc tggcgggggg gctcggcccc
     4981 aaggcggtga agatgttcga gggtttcggg atcgaggtct tcatcggggc taccggtacc
     5041 gtccgggacg ccatagccga ctggaaggag gggtacctgg aggaggccac cgacgagaac
     5101 gcctgccggg agcaccgcca ctgattcccc ggacgaatcc ggaaaggggg gggatgaatg
     5161 gccccctcct tgggcgatcc tctattttgt aatgctgacg atcatctcgg cctccaatgg
     5221 caatcctcaa atcatatctc gttctcaccg acttcttagg caaaggtgat cgagattgga
     5281 ttttgaggaa agcggccagg agatgacgat gatcgacgag ctgacggagg cgctctactc
     5341 gtgcatctcc tttcggaagg gggagcggcc cgatctccat cggctgaaag gcctctttat
     5401 cgatgaaggg atgctgataa acaacaacga cgaccccctg atcttttcca tccatcagtt
     5461 cgtggatgcc atcgattgtc agatctccgc cgggagcctc acctccttca gcgagcggga
     5521 ggtggcggag aggaccgaga tcttcggaaa gatcgcccat cggttcagca cctatgaggc
     5581 gaggttcgac cccgaggatg cggatcccat ctccatcggg atcaacagca tccagctcgt
     5641 gaaggtggaa gggtcctggc gggtgagctg tctcgtctgg aacgacgaga gggaggggcg
     5701 gaggattcca gaaaggtacc tctgaacttt gggatggatt aaaggcctaa gaggcgaatg
     5761 acccttttcg tccctttcat atctccatcg ggtccccgtt cccgctcacc tggcagacgg
     5821 ggcaggcgtc cttctcctca ttaagctccc tcgtggccat ggcgaggacg gtggcgttgg
     5881 cgatgttccc ggcgatgagg agggcggcgc cgatctcgtc gtcggtgatc cccatccgtt
     5941 tcgcgaccct gatgtggagc ttgaggcagt ggctgcaccg gatcgccgcg gcgacggcga
     6001 tggatatcag ctcctgggtc tccaggtcca gggcctgaga gcttcttatg atggcgttgt
     6061 tgtggaggat cttggtgatg aggagctcgg gcttgtcctt catgaacctg aatatgtagg
     6121 ggacctcccc gtacgccgtc tcgatcgatt cgagcatatc ctccgccgtc tgctccttcc
     6181 ccttctccag aacccgttgc agatcctctt caaagctcat aaaccctcac ctgggcccaa
     6241 agagccggct agaccacaaa gccggtctcc gaggtgggca atatccgaac gagtatatag
     6301 tccttcatcc tcgacggcat cacccgcgct atctccccca gggtaacccc ctcctcgaac
     6361 ctgacccccg ccaccgcctc gtggtactcc tcataaggaa gcttaatcct gaggccgttg
     6421 aggttgaggg cgatcgggag cggcgaatgg gagatgagga cgtcggggac ctgctccctc
     6481 accgcgtcca tcaccctcgg gggggtggtg gtgagggggt ccttggagat ctccgccctg
     6541 acgctgtcaa gggctctccc caccgcggcg actatgttgt cgaggcttcc gatctccgtc
     6601 gccttccggg aacggtgggc tatcctgccg acgtttggga cgtactcgaa ggcgtaggga
     6661 aggtcctcct tcctcatctc ggggccgccg gtgacgatca ccgggacggt gagggtccgg
     6721 tagaggtgct ccttcttcag gatgcagtcg gcgaagttcc cgaaggtgaa gaccacggca
     6781 tcgtgctcgt tgatgaggcc ggtctcgtac tcgtccatct gggcgatctt cttcgccacc
     6841 ccccggctca tcccgatcat gttgctcttc gccccgtgcc tcctgaggta ctcggcgacg
     6901 tcgcaggcgg ggtgggggag gtgatggatg gccagggtcg gggtcaccac ggcgatctcc
     6961 gtacccgcca ggggcgcccg ttccagcttc cccttcagct ccttggtcaa tgcctccagg
     7021 accggcatgt cctcgtgggg gacgagcatc agaaggatca cctcgctctg ggtgacgttc
     7081 ttctggagga ggtaccctcc caggtcctcg acgagctccg tcaccaggtc gtgcttgtag
     7141 acgccgccgg tgaacatcac gggatcaagc atctcgcatg cctccgatct tccccttcgc
     7201 ctcggtgacc agctcggggg ttatcgtctc ctccgccgcc aggaagaaga agctctcttt
     7261 tcccatctcg ttcctccggt tcctgaagcc ctcgggggat atcctgacga gggcgtccat
     7321 caggtcccgg cggaactccg tctccaggtc cgccaccacc agctcctcgg gggattggtc
     7381 cgataggacc accagaacgt tcctgtcggg ctgctccacc ttctcccggc cgtaggcctc
     7441 ccagagggcc cggatgaggc cggccatatg ctcctcgtcc tccacggtga tctggtagcc
     7501 gccctcggcg gccaccagat ccccgacgtc cctcaccctc agggggggga gcttcccccg
     7561 gatcatcccg aagacggcga agtaggggat gaggatgtcg acgtagacgt agagcctccc
     7621 gatcgacctg atcagcccca gatcctggag aatgtccatg atgatctctc gatacttctt
     7681 cgccccatac tcctccccgg gcatctggac ctcgaagacc tcgagggggt ccatctccat
     7741 ctcagtcctc cacgagcccc gagacgagga ggcttgctcc gacgcaaccg atgtactggg
     7801 cgtactccgg gacgatcacc ttcagcccca ggagctgctc catcgccctc gggagaccct
     7861 cgatgagggc ggtcccgccc accatgatca ccggctcctt cacctccacc tcctggagct
     7921 gctgctcgaa gacctgctcg gcgacgctgt ggcaggcggc catcgccacg tcctcgggcc
     7981 tggaccccgc cgagaggctg ttcaccaggc tctgggttcc gaatactatg cagtagctgt
     8041 tcatggggac ttttcgatgg tctcccttga gggcgagctt cccgagctcc gtaatctcga
     8101 ccccgagcct tctggccgtc agctccagaa acctccccga ggccccggcg cagatcccgc
     8161 ccatggtgaa ggttcccggg accccatcct ggacggatat cgccttgttg tccatccctc
     8221 cgatatctat caccgtggcg gcgcccttct gggaatctgc caggaagacc gcccccttgg
     8281 agttgacggt gatctcctct tggatcagct ttgcgccgaa ctctttgccg acgaggtggc
     8341 ggccgtatcc ggtggttccc agcccctcga tctcccccct ctcgaccccc gccgattcga
     8401 gggcggcatt gtaggccgcc tcggcgctct tgagaacctc ggtggtgggg acccatccgg
     8461 tccctattac ctcgttatcc ctcatcacca ccgacttggt cgtcgtcgag ccggagtcga
     8521 tcccggcggt gaggccggtc tgcctctccc gacttaacag acccttgaac ttggcggtgg
     8581 tgacgagggc ctccatcctc gtgaggaggg tctcggccgt cgtcctctcg gtgaaggagt
     8641 agctgaggac ggggatcctg gagttctcgt ggatgtactt ccgcagctcg cttctgacga
     8701 tcgccccctc ggcgcaccga aagcaggtgg ctacgaagac ggcgtcggcg aggtcggggt
     8761 tctccacgat cctggtggcc ctcgccatgg ctaggttcag atcgccgctg gccacctcga
     8821 tcccgaacct ctcctcgacg ttcctgacgt cgtctaggga tacctcaggg aagatgagct
     8881 cggcccccac catctcggcg gccttctcca gctccccctg gacgccgctg tactcggggc
     8941 cgcaggagag ctgggctatt cttatcaact caatccctcc agaaactccc ggatcttcga
     9001 gacgaatcgc cgggctgcca gctcatcccc gggaaactcc atctccagga ggggtatcct
     9061 cttggtcctg aggaggaagc gggtcagctc gttggtcctc gcgcatcctg tgcaccccag
     9121 ggtgcaccgg tcgttcatca cgacgatcgc cgcctctgcc tcctctatca tcggaccgat
     9181 gagggccatc cgacccctga cacccgaggg gacgtcgacg gcggcgtact tcagcccctt
     9241 cttcggttcc tccggggtca tgttcagggg aggggagtcg agaccggggg tgttgaccat
     9301 ctttctgacc tcttttgata ccaccagcgc ctcgtggccc gtcctctcga cgaggtccgc
     9361 gaggatcatg ctggtggggg ggtaaaccat gactttgacc atgaaccaac ctcaggaaaa
     9421 ctcctcgttt atcatctctt ccagctcctc cagcccaggc cgcttcttcg cctcggcgga
     9481 gggcggggcc tcctcgcccc gaaggtcggc ctcgagggcc cgggatatca ggggcaggat
     9541 ctcggactcg tgctgcatca tgtagaagcc gggcctggct cctccgcctc gatgcccccg
     9601 acacctcctg ggatccccgg gcggaaagcc ccgatctttg atgaagatgt tgctctcgtc
     9661 catcttcctc agctcctcca ccgtcgccct gatgtcctcc tcctcgccgt cgatgatgac
     9721 gccaaagcag gtctctttga cgttgatatc ccggccgagc tcgtatatct tgagagcgat
     9781 gtcggagggg aggacgtcgg actcctggct cgtcaccacg tacttggtag ccttcaccta
     9841 tcgcacctcc agaacgtaga cggtctcctc ctccctgacg ttccggagct tctccaggtc
     9901 gaggatccga ccgacgatgt tggtcgcgag gaacttctcc cccgagggtc cgtacctggg
     9961 gtcgtcctgc agcttcaccc ccacgaggcc ggcgttcttc gccgcctggt tggtcactcc
    10021 gatctccccg gccctgacca tatcgacggg cttgttctcg gggaggatct ccttgtagct
    10081 tgtggcggag atggcgggct tgaagaggat ggtcttctcg tagacgaagt agacgggcag
    10141 aggacccacg ggcttctctt tcagccctac cacgtgcctg aagtagtcca gggtcttggg
    10201 ggcgaggtcg tcatagagct cgatcgccac catccggtcg gcggggatcg aggtgacgga
    10261 gacccgcccc tccctcagga tctgcatggt ggtgacgggc ctctgctcca ccaccacagc
    10321 gtcgtcgccg gtatatcctt cgacctcaaa ctccagcccc cggtcggtgg cggtccgctc
    10381 cgcctctttg agggagagcc ccatcagcat gatcctctcg ggcttcacct tcgccgcgag
    10441 cctcatcccg gggcctgcca gcttcaccag gtccatcccg gaggtgacgg tccccaccac
    10501 cgattggctc ggggtcgacg tcctgtccgc cttgtagatg aagatccggc cgaggcccct
    10561 tcccgtggtt ctgatggtga cggccccctc ggcgcgaggc tcccggttct caaactcgat
    10621 ccgctctccc aaaaggacgt cggaggacgc gtaagagctg gaggtcccgt cgacggcgaa
    10681 gaccccgtcc ctcgtcgccg ccaggaagaa ctctgccccc tggggggcct cctccaaaag
    10741 ttcgacctct atcctggtgt agatctccat cccgtcggtc agaggtagcg agaggtctcg
    10801 ggtcaccgtc ttatcggaga gggcggacca ttccactatc ggctccgcct ccaggatggc
    10861 gtcgcccttc tccagccttc ccagcaggtc ttttcccccc accaccgcag cgaagacccc
    10921 ccggttttcg ccgggggcgc cgtaggctgc cgagtgcctc ctcctggcga agatcacctg
    10981 ggtcttatct gcctcaaagc cgctggtgcc gaatatcacc tcccatcggt cgtactcgtt
    11041 ctcgccccgg gtgggggcga tcgccgttct caccggcccg aaggcgactg cagcgccgtc
    11101 gctccaccgc gcctggaggc cggatatctg gctgaccgcc tggtgccatg ggcccaccag
    11161 gcccgtatcc agaagctcga tcctcagctt ccccttggtg gtgttcagcc agtaggagtt
    11221 ggtctcccgc gcctcctctt cccggccccg gacgatcccg atgatcgccc cctcccccgg
    11281 tgacgcgccc gccgcatcca gggcgtcttt cagggtcgag ccgtccggaa gggtgacctc
    11341 cgccccgtcc acaaagacct tcaagttaac tccccagcat ctcctcttct tcctctttgg
    11401 tcatgagcga gaggacgccc tcaggggttc ctacgtggac gatcttcccg ttccgcatca
    11461 aggccgcccg gtcgcagacg ttctggacga agtccatgtc gtggctgacg atgaggaagg
    11521 tctcgccgag ctcgtccctg gcgttgagga cggatttgga gacgtcgatc ttggtgatga
    11581 ggtccatcgt ccccgtcggc tcgtccagga ccacgatcct cggctccttg atcaggacct
    11641 gggccatggc gaccctgtgc ctctccccct cgctcagctc gttggggttc ttggtgagga
    11701 ccttctccgc ctcctcctcg gagaagccga cggtcaccag ggtgtggatc gccttcctct
    11761 cggcgagctc gaaggggagg gagagcccga tcgactcggt gaggttgtcg atgacgctcc
    11821 tgtaggggta gagggtgtac tcctgatgga gcatccccat gtacttcgtc gccctcccct
    11881 tatgatcggc ccccaggatc gtcatgtcga cccagtcatc gccgaccctg caccagatcc
    11941 cgccgctggt gggcatgact atgccgttca gcatcttgga gagggtggtc ttcccggccc
    12001 cgctcgtccc tacgaggccg aagatctccc cctcgtagac ctccagggat acctcgtcga
    12061 cggcccggac gacgccgcgg tctatgctga tgtaccgctt ggagaggtct ttgcaccgga
    12121 tgatcggcga cccgacgtcg gtcttcttcc tgtcctcgat cttcccggcc atggctacga
    12181 actttccggc gatcttggag ggctcgccga tggagacgat ctcgccgtgg tccaggagga
    12241 ccgccctgtc cgtcagctcc accatcacgt ccggccagtg gctggtgatg atcatcgtca
    12301 tgttgaagtc gtccaccgcc cgcctgatgc tctcgtggac gaggtcggcg gtcttcgggt
    12361 ccagggtccc cgagggctcg tcggcgagga gcatcatcgg ggacttggcg agctgccggg
    12421 cgaggacgac cctctgcttc tcgcccccgg agagctccct cgccacgtgc atcatcctgt
    12481 gggacatgtt cacctcgtcg aggaggtctg ccgcccggtt gacgctgatc gagccgccgc
    12541 tcgcctcctc tacggcccgc atgacgttga cgatgaccct ctcgtcgccg tagagggcga
    12601 aggtcctctg aagcatgatg gcgatcctgc gagatacgtc cctcctcttg ggatcgttga
    12661 tcccgagaga tacgaagtcg gcttcgaagg gctggaaggt cccgccgcac ctgcagctct
    12721 cgccggccct gctcggcggc tcgacatggc tgcactgggg acatcttgct acgtggtaga
    12781 tgacgctccc ggtgatctcc tcgaagggct cgacacctcg aaggacgtga aggaggacgc
    12841 tcttccccga tccgctcctt cccaggatgc cgagggactc cccctcttca atctccaaat
    12901 tgacgtggcg aagtgcttcg acttcgccaa acttaaccgt caagtcccgg atctcaataa
    12961 atggtgtcaa attatcatgc ctcgaattgg tattgcaaac gaactacgaa ccatcccgaa
    13021 tttaagttta tccgagaccg tcccgccaga gaccgccgtc atcgatcatc aacttcgatt
    13081 acatatatca tccagccatg gcccggcctc tcgatgtagg aaagtctcct ccgacgaccc
    13141 gctgaagatc ttcgggcttg atcgggcgat ccctatccct gccctccctt cttgaggaga
    13201 aactctttgt ccaggtgagc cttgtgagca gcctcgagac attccttaca tacgtgaaaa
    13261 accgtgtcct tcaggccgtg tatggtagct tgtttcgctt ttacaggcag cgtctccttg
    13321 tcaacagcac agatttgaca tttcatccag gtcacctctt tccaatctgg ttttcgtcgt
    13381 tataagcatt tccgctcagg ggcgtcctcc accgctggta aaggtcgtgc tcaacctcca
    13441 ggaggtccag gatcctcccc accaccgcgt ccaccatctc ctcgatcgtc tccggcttgg
    13501 agtagaatcc cggagacggc ggaagtatga tcgcccccgc ctccgtcgca gccaccatgt
    13561 tcttcaggtg gacgaggttc aggggcgact cccgggggac gaggatcagc ctcctcctct
    13621 ccttaagaca gacgtccgcc gccctggtga tgagggtatc ggataccccg ctggcgatcc
    13681 ctgccagggt cctcatgctg cagggggcca ccaccatggc gtcgaatttg caggagccgc
    13741 tggcgataca tgcggtgaag tcccggggcg agtagacccg atccgccatc atctccacgt
    13801 cgatggggct ctcctccgtc tcgatctcga tgatcctccg ggcggcctcc gtcatcacca
    13861 ggtgggtcgt ggagcccaaa tccgccaaga cctccaggag cctgatcccg taaacgaccc
    13921 cggaagcgcc gctgatcccc accaccacgt ccacaacttt tcccgtcctc ttaatggtat
    13981 cacatcctcc tactaattgg ccccccccat cagcccctcc cggctagccg ggtcagggtc
    14041 gaggccacca tcagggggaa gttgatggtg gcgtcgccgt agaccgtcac cttcctcgct
    14101 cccggggaga tcttccccca ggataccgcc tcggagaggg acgcccccga gagccctccc
    14161 gcctcgggcc tgtccgtggt gagctggatc gcgaggtcga agctgttggg ggtgacgagc
    14221 atcgcctgga gggcgaagtt cttggggacc ccccctccta ttattactat cccggatcgc
    14281 tccgctccgt agcagatatc caccagctcc cccacatcac cgaagaggtc gacctccagg
    14341 gggtgggtct ggcggaagag ccaggcctgc agccctatca tcgagtccga gagggcggga
    14401 cagaaaacgg gaactcttgc gtcggcggcc gcccggagga tcgacctttc gtcctcgatc
    14461 ctgcttccca gaactctcag aagctcggtc cccgtcatat ccttcccctg ggggaggatc
    14521 tcctggagga gctcctcgaa ccggacgaag tcgtcgtctc cgacgtagac gtcgtatatc
    14581 ctgctcaccc ccgactccct cagctcggag tcggaggcct cggtatctcc caggacgtgg
    14641 tggctgaaac cctctattat atcgtggacg aggtttgctc ccgtagtgac aaggacgtct
    14701 atcctcccgt cccggatgag ggaggagagt atgttcctca tcccggcggg gaccatcgcg
    14761 ccggccaggg tgaagaacct ggtgtactct cccgactgca tctcctcgta gatatttacg
    14821 gcttcggcaa gcctcctcgc cccaaagccg caaccctcca tcatcctcac cagctcgtct
    14881 gcacccatcc cggcctttat ctccatctgc cttatataat ccacctaaac tcacctatat
    14941 tatgccagtt tgaagccttt taggaccgac cgcatcactg cttagcggta gtgatgctaa
    15001 tcgattttct cataaacctt ttatatcagt accttgtgtt tagctcttac ggctaatgga
    15061 ggtaaaaaat gagcggcgaa ttggtggcag tggactatta tatcccactt attatgtttc
    15121 tgataatagg tgctcttgtg cccataggcg cgctggctgc cgtgaagatc atatctcctc
    15181 agaagtcaac ttttcagaag cgggcgacct acgagggcgg gcttagaccg atacgggagg
    15241 cgataatcca gtacaacgtc cagtattatc tcttcgccat cgtcttcgta atcttcgatg
    15301 tggaggttct cttcctctat ccctggatct acgtctacgc ttccgaggcg atccctgcct
    15361 tcggcggagt gagcattgcc atcaccggga tgctgatttt catcgtagtg ctcctgatcg
    15421 ggttgctcta cgacattaag aaggaggcat tgcgttgggt ataagtgatg tcatacctct
    15481 tcctgttgct gttgataagg agctggagaa gtggacgatc tgggggcggc ccctgacgga
    15541 cgtctggttc accaccacgg agaagatcca cgagatcatc gatatgggcc ccat
//
LOCUS       DASM01000046            4380 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_13323, whole genome shotgun sequence.
ACCESSION   DASM01000046 DASM01000000
VERSION     DASM01000046.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_13323"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          774 a         1357 c         1391 g          856 t
ORIGIN      
        1 cgccgatgat catgctgctg caggtcccca ccaccccgat cagcttagga tcgaagagct
       61 ccgtcgccct ccggaggagg cgggtcagct gctcctgccc cccgaagacg aaccccgcct
      121 cgtcganncc gtcctcctcc aggaggcggg cgtgcttgaa gcagcagccg ctggggccgt
      181 gcattataac gacgtccacc cccaggtctc ggagggtata aagggccgcc acgatggagc
      241 tcggcctcgg atgaagtatc tggacttccc ttgccatcat caaagatcac ctgaaacatt
      301 tgacgcatga aactaatcta tcgcccctct ccaggagggg cttcgccctg gcgagggccg
      361 cttcgaggtc gggggcggtc tcggccgata ggcggggggc ataaggggcg agcctctctc
      421 ccaccaggat caggaggtcg atctcccggc ggcggcggtc gaggaggtcg atcagggagg
      481 ggacgtccat cccctcgcag accgtcgccg cctcctcccc gacgacgagg gcgagccgtc
      541 cgccgacccc ggcgaggtcc agggcctccc ccacccccga ggcccggagg ccggagttgg
      601 agttgtcgac gaggaggacc ccgtccaccc ggccgacctt catcctcccc ccgagcccct
      661 cgaacccctc cagggcggcg gcgacctcct cgaggtcgaa gcccgccgcc agggcggcgg
      721 cagcagcccc cgccatcgcc ggagcgtagc tccggaggtc gagcccctcg ccgaggcgga
      781 ggggggcggc tccgcccccc gccaggatct cccggtccga ggcccatacc cggcccctgg
      841 ggccggggcc gaaggtggcg tccgctgaga ccttcacgtc ctccccggcg atcagcttcg
      901 acccctcttt cgagagggag atcatctgga gcttggcggt gctggcccag agtgcttccc
      961 ctccaatcct gtagtctccg gcgaagctgg tgatgatccc gatctcggcg agccccgtcc
     1021 cccccaggga gacctccgat acgagatcgt ccgccccgac ctctgcggca gcctcccagg
     1081 cgaggattac gttggcgggg gtgatgctca gcttcgacct caagaggcgg ctcctgccct
     1141 ccctccagac ctcgatcccc cgggtggtgt gggagaggac ggtcccccgg gcagagagga
     1201 tctgggcgag gatgagggcg gtggtggtct tgcccaccgt ccccgtcacc tcgaagggcc
     1261 tgaattcggg gtcgccggcg aggagctccc ccacggcccg atggtgggtg atcgtctcct
     1321 tccccagcct cctcgcctcc gtcagggcgg ggttcttagg cgggaggtgg acgggggcga
     1381 ctatcaggtc gaagggcgag acctcggggg cggcatggta gacctccagg gccgccgcct
     1441 cgatcccccg ccctctcatc cgctctgcca aaagggtggc gccgtggatg gtgtctaaga
     1501 ccgccacctt gggggctact tcagagcctc ggaggctctc cgataggcga gggcggcgat
     1561 ggactcgtcc acccccaggg gctctgcgca cctgatctcc acctcggtcc cgttcagctg
     1621 gcacttgccc ctcctgtggg tcgggtcgac ggagagctcc gtggggatgt cctcaagggt
     1681 gtggacgccg tgggcgagga agatcgggag ggcgacgatc ctcttcaccc ccgtccccga
     1741 gaagctctca aggcctgcgg cgatattcgg ctcgtccatg ttcagaaaag ctatcctgac
     1801 ggggccttcg tggaggtcgc cgatcatctt tgcgcacttc tccaccatct ctttgttgta
     1861 cggcttggtg ctgccgtgtc caagaaccag aatccctaca tcgtcggcca tgtatctcac
     1921 caatgttacg tttttatata tttgtattaa ccagatgggg ttaataacta ataaatcctt
     1981 tcccggtgta aaggggtccc gccgaaacct ccccggatat ccgccggagg cagggatttc
     2041 agacggcgac gatggggcag aacaattggg ataagtcgaa atgagccgca tagcgtatct
     2101 cctggcatca gttcttttag ttatccagac agccggcggg gccgaggtcc tgatgtcgac
     2161 gcgagagagc atcaccttcg ccgagaacct gacggtggtg gtgatggact tggagccgag
     2221 gacgggggtg gtatggctgg agctccagga ggatggcgtc cccctcaagt cctcgatcct
     2281 gaggacgggg gagagcttcg cctacgacga gaggggcgac ggcctgaacc tgaccgtcgc
     2341 caggatctac gccggggggg agagggacct ggtggacctg gaggtggtct ccgggaaggt
     2401 cgtcggaggg gggggcgagg aggacctcag cgcctcggcc aaatacgggg agcggacggc
     2461 ggcggcggac tcctccctcg ccaactgggg gcttgtcggc ctcctcctcc tcctcctcgc
     2521 cgcctgggtc tacttcgggg cgaggaggag gggtgtgtga gattggtcgt cgattgccag
     2581 ggacgcttta agataatggt gtaaaaaaat ggtccggacc tcggcagggg tcgggccagg
     2641 gtcaaacccc gccctttcgc cgaagaccgg ctcacccgtc tattcatcga ttggcgtcag
     2701 gatggtggtc tcggcgaagt atccgttgaa ggtatagtcg tcggcctcct ccggatccag
     2761 gccaggtttg atgatgtatt catagcctat gcctttgagg acgatctggc cggactcgag
     2821 gatctttcct tccaggaccc cctctatctc gtaagtgacg ttcgtttcga catccgttcc
     2881 ggtcccgaag tagaccaagg agagctcatc catgatggtg gagccgagga tggggtagga
     2941 ctcgccatca gacgactcga tggtacctgt gaggatcgag ccgtgctgct cgatcgtcca
     3001 gatgtaggtc ccctcctcct ccaggtaggg atggggcgag gtatcatttt catcgagctc
     3061 gatgtaggcg ccttcggtga tgtcggcata ccttcccgat atatccctca cctccaccat
     3121 cgtggagatg gcgctggcgg tcagcttcgc ccagggatct tcgccgtcgg cggccacctc
     3181 agcctcctct tctggggagg agatctcact cgcattcagc tcctccaggg tctcttcaac
     3241 ctcggcagct tccgcagcct cttcccctcc ggcggttaat cccaaaagca gaaggagggc
     3301 caagatccct cccagggtcg cagatcctct catctcatta cctcctgttt atttcctgaa
     3361 gttatctaaa ttattgcttc gtaatgatgt aaatactttt tgatatatct ggaatgtttt
     3421 ttttaatatg aattggcatc gaggaggggc gggggatctc gcgaaggtaa catgccctgc
     3481 cttcggatcg ggctgccggg ggcgaggggc tgagggccca aggccccaag atgtcgatac
     3541 catcgaagtc cctgcctctc cttcgccgat ccgcccccgg atccgagggg ccgggtttga
     3601 tgacgtaaga ggctctgctg cagccccctc gtgggattcg tagaataatt acttataatc
     3661 ttaagtctaa accgatttgt gagatgcggc tcttcgccgc tttttatcga tggctaaagg
     3721 aggatcgaac ggtgaaatct tcatccagaa taattaagac ctctattctc gccaccctgc
     3781 tggtggccgc caccttcgtc ctggtggggg cggcttcaga gctccagaag ggggagctcc
     3841 tcttccaaga cgactttgag acgagcaagg tgggcggggc ctgggggtgc ctccgggggg
     3901 agaaccagag caactactat gaggctggca agtacatcat caacgtcgtc cccgccgact
     3961 cctggtggag ggtccagtcc ggcaggtcct tcggtgattc tatcgtggag gtggaggcga
     4021 ccccgacggg cggccccgac gacaacgtct atgggctgat gaccggctgg cagaactggt
     4081 ccaactacta ccttttcatg atctccgggg acggctactt caggctcagc aagagggagg
     4141 ggggcaactg gacccggaac gacccctgga cgaggtccga ggcgatcaag ctcggaaagg
     4201 cgacgaacct cctccaggtc cagcggcagg gggagacatt atccttctac gccaacgggg
     4261 tcaagctcgc cgactacgtc gacggatcct tccccgaggg gaacgcaggt ctgatcgctg
     4321 gcacgacggc ctgtggcggg gggaacttga ccgtctcctt cgacaacttc tcggtctggg
//
LOCUS       DASM01000047            4184 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_52347, whole genome shotgun sequence.
ACCESSION   DASM01000047 DASM01000000
VERSION     DASM01000047.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_52347"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          731 a         1247 c         1398 g          806 t
ORIGIN      
        1 agcccgccgt cagagcggca gcggtccacg ctctcggcga gatgggagat gatccgaggg
       61 ccatcgacgg cctaatctca tctctcaaag acgacgatcc tgcccttcgg atatatggag
      121 ccacgatcct cgggggcctg ggagatgaga gggctgtcag cccgctgatc gatcttctca
      181 aagacgacga gccggcggtg agggcgatgg cgggggtcgc cctcggggat atcgggagcg
      241 agaaggcggc gggccccctg atagccctcc tcgaagatga ggcgccggag gtgagagccg
      301 gggccgccca agccctggcg accgtcgggg acgagagggc gacaagacct ctcatcgagg
      361 ccctgggtga cgaggaagcg agcgtcagga gctgggcgtc cctcgccctc ggctccatcg
      421 gcggccctga ggcggtcgat cccttgatcg ccggactcgg cgacgaggat gagggggtga
      481 ggaccgtggc ggcctcggcc ctggccgaga taggcggcga tgccgtcgat cccctcataa
      541 aagctctcga ggaagaggac ccatccgtcc agatctgggc tgcctgggct ctgggggaga
      601 tcggcgaccc gaaggccgtc gatccgctta tagaggctct gaagaacgaa gatcaggcag
      661 tgaggctgat ggccgtcgtc ggactcgcgg ggttcgacga cgatagaagg gcggagccgc
      721 tcatcgccgc cctcgccgat gaggacgagg tggtgaggtt tgcggcggcc gcgaccctgg
      781 ccgagatagg agatccgagg gcaaaagagc ccctgaagag ggcgctggag gacgaggacc
      841 ccgatgtccg caggaaggcg gcggaggcgc tcgataggct ggagggggtt taggtttcaa
      901 aaggaaggct ctttgcggga ttatcggtga cctggatgaa gagcaacggg gtgatagatg
      961 agatgaagac gaggatccac cagccggatt tcgacgtagg tcagttcgaa ggcttcgtcc
     1021 tcggcgggga cgtcggaggg acgaatacca acatcggcgt cgccggggtg aggtacggaa
     1081 tggccaccct cctctactcc gcccacttca agagccgaag gctcacctcc ctcgcaccgg
     1141 cggtccggga ggccctcgat tatggccggg tgaaatacgg gatcgaggtc gtccggggcg
     1201 tcgtcggggt tgcgggtccc gtcaccgacc ccaggagggc gaagcctacg aacctcccct
     1261 ggaccgtgga cgccgaggag atgaggaggg agttcggctt tgaggagttt aggatagaaa
     1321 acgactttat gatcatcggc cgggggatcg aatccctgaa agaggccgac ctcttctctg
     1381 cgaagggggg cgatccggcc ttcggcgacg gacgggcggt catcggcgcc gggacgggcc
     1441 tcggcaaggc gatcctggtc tatgacggcg aaagatacag acctgtcccc agcgaagggg
     1501 ggcacgcgga ctttccggtc cgagatcagt tcgacctgga cttgacagac catatccggg
     1561 gcgggagaga tacccccgcc agctacgagg acctcctctc ggggcggggg atcgagggga
     1621 tctacgattt tttaaagggg aggcgcggtg ggacggatct gaccgccgag atcgaggggg
     1681 ccgaggataa agccgcagcc atctcgaagc agaggaagac cgaccccctc tgccgcgaga
     1741 ccttcgccgt ctacgcgagg tgcttcggca ggtgcgctaa aaacttcgtc ttggaggcgg
     1801 tcgccaccgg cggcctctac atcgccgggg ggatcgccgc taaaaataga gaggtctttg
     1861 cgtcgacaga gttctccacc gagtttctga acgccgagaa gcagcgccac cttctggaga
     1921 ggacgccggt tagtgtcatc acgaactatg acgtgagcct atacggggcc tgcaacgctg
     1981 ctgttcaccc atctgattga tttttcggtc gatctcaatt ctggtcccag gctcttcagg
     2041 ccaccccctt gcacagctcc ccccgctcca ggtcctctct gaagtagtcc cggggggtct
     2101 tgaggcccag gaggaggccg accttccttt tggcgtagaa ggggacgagg cgcagcgtct
     2161 cccgcaggaa ccatttgggg tagagccgct tgatcttggt gtagcggaac caggccaggt
     2221 cgtagtactt cgcgttcatc tcccaggtga tgtaggcgac ctcctcggcg gtgaggtgct
     2281 tggtgttcag gttggcgtgg aggccgtcgt agaaccggta gtcgtcgcag gcgatgagcc
     2341 ccatctcctt tagttcttct cgcatctccg tcttggggta aggggtgctg atgtagaaga
     2401 tcggtatgtc cactttgagc tttcgagcca gctcgaagtt cgaccagagg tcctcggcgt
     2461 catcctcggg gtttcctccg atcagccccg cggagacgat gatctcgtta tcgtgcatgt
     2521 actcgatcgc cttgtcggcg ttgtcggaca tcttcccctt cccnngcggc ggccatcttc
     2581 tcgacgagcc ctcggctcga cccgatcccc cgggccgagg cctggacctt gtacttgagg
     2641 tgggagaggc cggcctcgac gatggcgtcg cagagccgct ccatcctttt gacgtccagg
     2701 gtcatgttgt cgtcgggaaa gaagatcgac ttcgccccgt gggcctcggc gtcccggatg
     2761 tcctccagga ttcgatggaa ggggtacctg cggaaggatc ggccgtacat ctgctggatg
     2821 gagcagaact tgcagccgtt ggtgcacccc cggctcgtct cgacggagtc gattggtatg
     2881 ccgaagctct gaaatccctt ggtgatcagc cgacagtcgc ggttgggtag ctcaatctcc
     2941 gagacatcca ggacggggcg ggcggggtta tggaccatct tatcccccct ccagtaagag
     3001 aggccccgga cgccggcgag gtcgcggcca tcttcgatgg cttcgatcag ctcccggaag
     3061 gtcgcctccc cctccccccg gacgatgtag tcggcgagcc gcccctcggg gcttgccgcg
     3121 atctcttcgt agctgagggt cgggtggtag ccgccgaaga ccgtggcagc gccgctctcc
     3181 ttcgccatct tggccagggg gaggatggcg tggtactgaa agctcatgca gcttaagccg
     3241 acgaggtcgt agccgttcct caagatccct tcgagatact ccctcggcct cttcactccg
     3301 tggaggtcgg cgacgtcgac ggtgcagagg tggtcgacgt ttccggcgat ggagacgagg
     3361 ccgtagctgg ggatccgcgc gatccggtcg aagtcgggct ggacgaccgg cgaggacagc
     3421 agtagtacct tcatgttacc gtctcgcccc cggctccaag aaccacggct tctcggggtg
     3481 gaagtcgaac ctgttcgccc gggagacgta ggatgcgacc tccttcatcc cgtcgatccc
     3541 ggcgtaccgg taggcgttcc tccagacccg gtaggaggtc cgcaggggcg tcagccggtt
     3601 gtagacgcgc ttgagggacc agctgaggat ctcctccatc tcctctggcg gtatgttcgg
     3661 atggttccag acgaggtgct tcatgtcgaa cttgtgccag tcctcctcga agatgccgta
     3721 ttcggcgttg agctcgtccc agagcctcgt ctggggaagc ggcgtcatga tgcagatctg
     3781 ggttatgtcc agctttagcc tcgcgagctc cgagatgtcc cttttgaggg actcctttgt
     3841 gtcgtcggga aacccgatca tgtagtagcc gacggtcccc aggccatact tctggaggag
     3901 gcggaccgtc tccaggacgt cctcggtccc ctccttcttg ttgatgtcgt cgagcttctc
     3961 ctggtggagg ttctcgatcc cgatcgccgc ccccgcaaac cccttcacca tccggggctt
     4021 cgccccgttt gccgggtcgg gcctcgtcct catctcgacc cattcgggta ttttcttcct
     4081 caggtagtcg gcccgggcca tgcaccccca gaccatcccg tactcgtcga ggaggtcgac
     4141 gactttgtcg gcatgcctcc ggttcgcccc gaagctctcg tcct
//
LOCUS       DASM01000048            8786 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_52349, whole genome shotgun sequence.
ACCESSION   DASM01000048 DASM01000000
VERSION     DASM01000048.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8786
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_52349"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1430 a         3005 c         2645 g         1706 t
ORIGIN      
        1 accgccgaga acttccccgc caccctctgg ttcgacccct acctgaacta tcccctcggc
       61 catcctatca cctggccccc cctcttcgat caggtcgtag ccggcgcctc cctcctcttc
      121 ggcgtccccc cggaggtggc gggggccctg gcgccgccgg tcctcggctc ggcggcggtc
      181 ctggtcgtct acttcctggc gaggcggacc ttcggggcga gggtggcggt cctctccgcc
      241 ttcgtcctgg cgatcgatcc aaagcacctg gccagaaccc atttcgggtt cgtcgaccac
      301 gacgccctgg agtcccttct gatcctgggg gcgatcctcc tcctcgcata cgccctcacc
      361 gaacgggaca ggaagctgaa gcttggggcc gccgcgggag ccctcatcgc cgccgtcggc
      421 tacacctggc tcggggctcc gatatacatg gtcgcgatcc tcatctacgc cgtcctccag
      481 gcggccctcg acctccggga tggaaacgac ccctcctcct ccatcctccc cctggcggcg
      541 gccttcggcg tcgccctcct cctcttcctc cccttcaggg gggaggcctg gctcgccccc
      601 tccttcttcg gggctctggg ggggatcgca gccctggggg ccctcctcct cctatcccgg
      661 ctccttcgaa gggaggggct tgccgccccc gccctcctcc ccgccgccgc gatcctgggg
      721 gccctcgccc tcgccgtgat cagccccttt tggggcgaga gcggcatcgc cgccctctta
      781 aaggaggggg cccgctactt ctttttgggt gggattgggg agggtaggat cctcgaggcg
      841 gttcctatat accggctcct cgatcccgtc tcccttcccg ccctgggcct cttcctcctt
      901 ctcctgggcc tcggagccat gatccgcatg agccgcctct cacgccaccc cggggatatg
      961 ctcctcctcg tcgtctgggc ggccttcacc ctggccctgg cggccttcca ggcccggttc
     1021 ctcttcgtct cctccttggc gggatcgatc tccgtcgccc tcctcttctt ctggggatcc
     1081 gacctgcttt ttgggaggag gggtccggcc ccaaaggccg ccctcctcct tctactactc
     1141 cttcccaacg ccatcggcgt cctggaaatt atgggaggag agccggagat caaagggggc
     1201 tggatcgagg ccctggactg ggtggcggag aatacgccga tgacggtggg gttcgaggat
     1261 gccgtcggat ccccgagcta ctccatcatg agctggtggg attacggtaa ctggatcctc
     1321 ctccaatcga ggaggccggt ggtggcgaac aacttccagg cgggggcgac ggattccgca
     1381 aggttcttcc tcgcggagga ggaggaggag gccctgacga tcgccgatgg gaggggcgtc
     1441 cgctacgtca tcaccgacgg gaagatggtc tccgggaagc tcccggcgat cgtccggtgg
     1501 atcgacggag atccgagaag ctacatctcc agcggatcgg ggatctcatc gccctatacc
     1561 agcagcttca cctcgacggc cctcttccgc ctccacctcc aggacggctc ggggctggga
     1621 gccttccgcc tcgtctacga ggcgggcccg tccccggggc aaagggatcc cgcccccgaa
     1681 gtgaaggtct tcgagcgggt cattggggcg aggatctcgg ggactacccc ttacgatggg
     1741 ccgatggccg cagccctggt gatcgcctca aaccagggga ggaggttcgt ctactccaac
     1801 gaggcgatgc cggaggacgg caggtacgag atcaccgtcc cctactcgac cgatggagag
     1861 gaagataccc gttccaccgg cccctacctg gtggggccgc tggaggatct cggcggggag
     1921 ggatcgaggg aggtcgaggt ggcggaggag gacgtagccc ttgggagggt cgtggaggtg
     1981 aacttctgac cgccttggcc atccgacctc acaacctccc tggggttccc tcagagcaag
     2041 agctggcagc aggtcttgat gcagatcccc gggacgaccg tcctcccctc ccggtccagg
     2101 gcctcgatca ccccctcgtt ctcggtcccg ggctggagcc agaaggtctc aaaccccttc
     2161 tccctcgcct cctgggcgag gccggcgacc gcctcgggcc ttcgaaagac gtcgacgatg
     2221 tcgatcttct cggggacttc cctgagggag ccgaggacct tctcccccag gatctcctcc
     2281 ccccgatgga ccgggtttat caggtatacc ttgaagccat agctcttcac cacctccgat
     2341 acgaagtggc tcggcctccc ctcctcgggg gatatgccga cgacggcgac ggttctgctc
     2401 ctcttgagga tcgctctcag catctcggga tcatcgacta tcggcgctaa atcacctcct
     2461 ggatctcaga agttatggtt gatctgattt gatctggtga agatcgacct atctccctta
     2521 tctacctctc gatcgatgga ggatgcggcg gaatttcctt tgggctctct atcctagtac
     2581 aatatttaga tatttttaaa gaattggacc ttgccaattc cgcatgatgg tttatatggc
     2641 ggaaaacgga taccctaata tagaatccga tgcgaaatag gcccatgaaa aaattgctga
     2701 cattatccct gttgggggtc ctcctcctcc ccggcggcgc ctcggcagcg tgggagaggc
     2761 tctgggagga gaaggtcgac tcggtcgtct taaacggcag gatctcctcc caggaagatc
     2821 ccgaatatac ggtgagggtc tttgcagtcc agagcgagac gggggacata tacaggtacg
     2881 ccgagacgac gggggagtgg acgaaggtgg gaaaccccgg aaagaagttc gtctcggcgg
     2941 gcggggtcga tagcaggctc tacggcctcg acccggcggg gatgggcgtc tacgagttca
     3001 gcggtacggc gatggagtgg acccaggttg gcggacccgc tgctgatatc tacggcggag
     3061 gcgggtttct ctatgccacg aaccccgagg acggctggga ggtctacagg tatacgggga
     3121 ctccctggga atgggtgcag ataggagagc ggtcaccgtc ccgcccgaat atgggccaga
     3181 ttgtggacta tatggcatca ggcggcggat acctggcggg gatgggcttc ccctacgaga
     3241 tgatcccgtc gaaactctac ctcctcgccg agagggtcaa ctctgaaggc gtcaccatcg
     3301 gagtcgtatt gaagtattca gggacgcccc taaactggga gatattacag ggcggaggtt
     3361 tggatttcga cgccctcttc ggcgacgggt tcaacgccgg cgcggtctat gcaagggatt
     3421 atcacacccg agagttatat cgttggtctg aggatgccga aagttgggag cgggcgatcg
     3481 atccagcaaa gatcgtcgtc gccgaccagt ccatcgacgg ctcttcggtc cataacctcc
     3541 tctaccggct tgaagaagat ggagatctat ggatgcggga cgacaccgta ggatcttgga
     3601 ccaagatcga tacttcggga tggagaaatc ccctgaaggg gatctacgcc gccggcggag
     3661 agctctacgc cctctcagag gcgggctacg tcttcaggta cgtcccctga ccctggagac
     3721 ccccctccag gcgaactttt ttgctatccg ccccttcact ccacgcccct tcctgtgagt
     3781 tggagctctg ctgtgccccc ctccggcatc gaccggtgct tcatcagccg ggcgagcctc
     3841 ttcccctccc ccctcttctc gatgaagatc gcagtcttgg tggcgtggtc catcgccatc
     3901 ccgccgaggg gcctgaggtt tccggtctcg atctccatgt agacctggtt tgtcaccacc
     3961 accgggatcc ggtgcctcct cgccatctcc tgcagctccc cgagctgggc ggcgagggcc
     4021 cgccggacgg gcctgttgtc atccccctcg aaggcggccc tgtagaggga ggtggcggag
     4081 tcgaggatga ggaggccgaa ctcctttcca gcgatcttgg tggcgtcttt aatcgaggag
     4141 tgctgctgct ccaggttcag gggctcgaag acgacgatct ttgcgcctat ctccttcgcc
     4201 ccctccccgg cgatctgtct gaacctctcg acggagaacc cttcggtgtc gatgaagatc
     4261 accttcagcc cccggccgac ggctccgacg gcggcctgga ggactatcgt cgtcttcccc
     4321 gtccccgcct cgccgtagat ctgggttatg atccccggct catagccccc gccgaggagc
     4381 cggtccagcc cctcgcaccc tgaagagagc ctctcctgca tgattctcca atcaccctat
     4441 ctccatccgc cgttccttct tcgatctcgc cggctcaatc tgacccctcg atcagttcct
     4501 ccagccggtc catcgcctcg atctcccggg ggccgccgca gcggttgacg atccggcgga
     4561 tcttgaagat ctcctccagg tagagatcgc tcttgagggc gacgtagcgg gtcgcggcga
     4621 cggcagcctc cacgacgagg ttgatccccc ggttgacggc ccgaaactcc ctctccagga
     4681 cctcgccctt gacgaactcc acctccggga gggcgatgtc caggccgtta ggctcgcacc
     4741 tgaagagggc ccaggcctcg gcgtctcgga gggtgacctc tccgtccctc tcgacgaagt
     4801 tctcgtcgga gatgtccccg agcgccgtct cgacgaagag gatcggatcg tgggttacgt
     4861 tggccacgaa cttcttttcc gacattatat tctcgtaggt gtgagttcca acgaagagcc
     4921 ggaccgatag ggtctcgccc gtcctgatca gccccagggg cgcagcgtta cgggttccag
     4981 cctcgttcac cgtggtggca ataatctcgt ttatcccgtc gaatattccc agctcttcga
     5041 tccttccaag atcgcgaatc atccttcgtc cctcccgaag atggcccccc ccatcagggc
     5101 tatgaagagg gccgctgcca tcaggtccgc cgttgacccg gggtttatgt cctcatccac
     5161 aaacttcctg tccagctcct cggccaggcg gagggtctct tcgacctcaa accccaggat
     5221 cgccctcgcc ctctccgacg cctcctttgc cctctccggg ccgaacttcg aggcgacgag
     5281 gctgtcgggg acggccgcca gggcctccag gtaggtgatc gccaccccgt cgttgaggcc
     5341 gagctctccg acccgctccg ccagaaggcg ggcgaggaga aagctcctct caaagcccat
     5401 cgaccactcc ctggccacca ggtcgtgggc cgaggagagg cgcatcagct ccaggagggt
     5461 cttcccctcc cttcggatct tatcggggga ggcggggtct ttcaggctca gatccgccac
     5521 ctccgccacc ctcgccccgg cgatctcgaa ggcggtgtaa aagtcgacgg cgtcctccgc
     5581 cgtcgtctcc cccaggacct ccgccacgga gcccctcaga tccccccctc tccccgccgc
     5641 cgccgccagg gggatgagga gggccaggga gccgaagtgg gtgttctggg agagccccca
     5701 ctcccgccag gatccgaccg cccggcggat gagggcgccc gtcggaagaa gccccagggc
     5761 cgcgtcccgg aaggcggggt aggcggagac ggcgctgatg aggaagtggt ggaacttcac
     5821 ctcctcgaag tcgtgggacc gatctacgtt ccccggcttc ggggaggtgg agagctcgat
     5881 cagcatcgcg agctgggccg cttgcgcaac acggtcggga ttcgtcgtca tgccttcagc
     5941 agatcctcgg cgagcttctt cttcagcctc atgtactcat cgggggagat gccgtgctcc
     6001 tccaggacgg cgtccctcat gaagcagggc ttggtgatct tgcagcacca ggtgaggctc
     6061 ccaaagcagg tgccgtctcc gggctccagg ggcgtccccc gggtgagggc gatcttcttc
     6121 tctatgaact ctcggggcga gatccccatc ttcttgaggg ccgcgagaag ggggcagctc
     6181 ttcaccggag ggcagcagaa ggcgaccccc cggaggtcgc ccccggagca gatgtgcttg
     6241 ggggcgttgt accagccgac tagctcctgg aacctcttga gggcaccggc gagccatccg
     6301 acgaactcgg ggtccgccct ctccgacagc gatatcatat ccgcccccat ggagaggagg
     6361 tccttcgcgg cctcgaagtc gccgacctcg gacattgcca agaccggcgg agccccgccg
     6421 tcggagacct tcctgacgac cttcagcgcc ccaggaccga ggccgcggag gtcgaggtgg
     6481 aggaggtcgg cccccccttc ccgggcctct cgggcgaggg gggagaggtc ctcgaggccc
     6541 ttcggcctca ccctcaacga gagggtcgcc ccctccgcct tggcagcccc gaccacctcg
     6601 gcgatcccct cggtccgggg gagctccacc tcgatgagcc acccctcctc ggccgccatc
     6661 ctggccgcct ccgcgaggtc atctagatcc cccccgaagg tcgagaaccc cagggcggag
     6721 gagctggaga tcctctccat cctgctcctt atggccgagg ggtcggcctg gtggaggtcg
     6781 aaccgcccga ggatgatcag ccctggggag ccgttgagcc cccccagctc ctgggaggcg
     6841 gaggcgatcg ccagggggtt attcagttca aggtatccga tcttcaacat caatatcggg
     6901 ccccaaggcg agactcctgg cagggatatc agcttttcgg cctgggctca tcaagttcag
     6961 aaaaaatgat aatcctgcgc ggtcacgcct tcttcgcgag gaccatcggc tttccgcagc
     7021 agaccagcat ccccgcgcca gcctcctgga ccagaactac gttgccgcag acctcacacc
     7081 ggtaaacttc gcccacttta gccaactcgt atcacctctg atcctcatct tatctccccc
     7141 ttgctttaag cctcttttgc cggagggggt ggtcactcaa accccctgac gaagcccttg
     7201 acgcagtgcc ggaggtcttg gtgatacccc ccctccagga cggcaaacct ccggccagag
     7261 cacctctcct ccgccccctc cttcaggatc ctgccgatct cctcgtagtc ggagaggtcg
     7321 aggaggccgc cccagtcgag gaggtaggtg tcgaagccgg cggagacggc gaggaggtcg
     7381 aattcagatt ctgcaacggc cctctccacc tggccgatgt agccggcggg atcgagggtg
     7441 aggtagtcga agccggggtc tatggccccg acgtttgcga tccgtaccct ctcatcccac
     7501 cggaatatgc tgaccgtccc gtcgccgaag tggaggtcga tgtccacgat caggaccctg
     7561 cccccggagc cgccccccct ccggcgtccc aaccctgcct ccttcgggcc ttccgcctcc
     7621 cctcccatgc cgccccccat cctatccagg gccatcctga cggcgatggc gacgttgttg
     7681 aggtagcaca tcccccaggc gtggtcgctt gaggcgtggt ggcccggcgg ccggaccagg
     7741 gcgaaggcgg cctcgccccg aagggcgatc tccgcggccc ggcaggcgcc gccggccgcc
     7801 agcagggcgg gagcgagcat cccctttccc ctcaccctct cgatatgctc acggctgtgg
     7861 atccgcgata catcctcgag gctcgcttgg gggggctcta cgaactcata gcccgccagc
     7921 tcccgggccg gaagccgcac ccggtcgggg ctctcgacgg ggttggtggt gtacctctca
     7981 gcgaacctct ctgagtatac gaccttcaag atctgacctc gatcggcacg gaagatcctc
     8041 ctctcttcaa tccgcctcca gcatcctcgg aggcccgccg tagtcgaagc tgttcgccct
     8101 gacgaggcag gatagcagca cccttatccc ggcgaccccc gcctcgccgt aggccttcct
     8161 ccagatcctc gccgaggacc gcaggggcgc cgtcctgggg ttcaccctcc tcatcgacca
     8221 gtcgaggagg acctccatct cctctggcga gatattcggg tggttccaga cgaggtgctt
     8281 tccgtcgaag tggtggtagt cgaggtcgga tatcccgtac cttccggcga cctcctccca
     8341 gagcttcgtt ctgggcagcg gggtgaggac gcagatctgg gtgaggtcga gcttgaggga
     8401 ggcgagcctt tcgacgtcct ctcttatcga cgccgcagta tcttgcggaa agccgatcat
     8461 gtagtagccg acggtcccca ggccgtggct cttcagtttt tcgaccgtct ccatgatctc
     8521 cccggcccct tcccgcttcg cgacgccgtc gaggacctcc tggtggaagt tctcgatccc
     8581 gatggcagcg cccgcaaagc ctctgacggg gttacggcct ttgccagccc cgcgagaggg
     8641 ccgcatcctc ctcatctctc cccactcatc gatcttatct atcaggtagt cggccctggc
     8701 catgcacccc cagaccatcc cgtactcgtc gagtaggtcg acgactttgt cggcgtggcg
     8761 gcggttcgcc ccgaagctct cgtcct
//
LOCUS       DASM01000049            7114 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_41163, whole genome shotgun sequence.
ACCESSION   DASM01000049 DASM01000000
VERSION     DASM01000049.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_41163"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1357 a         2114 c         2226 g         1417 t
ORIGIN      
        1 gggctcgccg gcggtgacgg cgtaccggag ggtcgagaag tcgtacctgg agatatcctc
       61 ctttatcatg aaccggtaga tggtgggggg ggcgcagaag gtggtgactt tgtacttagc
      121 cgccatatcc atcatcctcc cggcgtcgaa ccggtcgtaa tcgtagacga agaccgccga
      181 tcccgcgatc cactggccgt agagcttccc ccagactgcc ttgccccagc cggtatcggc
      241 gacggtgtag tggaggccgc catcctcgac gttctgccag tacctcgccg tcaagatgtg
      301 cccgagggag tagctctggt cgtggttgac catcttgggg tttcctgtcg tcccggaggt
      361 gaagtaggcg aggaggatgt cggagctctt cgtcccttcc ggcctctcga actccgggga
      421 tgccgcctcc agctcctccc tcaggttcag ccagccgtcc ctcgcccctt ctccgacgaa
      481 ggccttcagg accccggcat ctctcagctc ctgacaggct ccgtccacct cctcggggac
      541 cccctcctcg gcgatggaga tgatcatctt caggtccgcc cgccggaccc ggtagacgat
      601 atccttcctc ttcagcatgt gggttgcggg gatggcgacg gccccgatct tgtggaggcc
      661 gatcatacag atccagaact catatcggct cttgagggag agcatcacgg cgtctccctt
      721 ccttatgcag tgccccttga agaagttggc agccctgtcg ctctcgcgct tcatatcccc
      781 gaaggtcagg atcttctcct ccccggagtc gttgcaccag acgagggcca ccttatgggg
      841 gtcctcctcg gcgtagacgt cgacgacgtc aaaggcaaag ttgaactcct cggggactat
      901 gatcttgaag ttctctataa aatcctcata agatttgaaa tccgtcctgg ggacgaacct
      961 atccagtaga gacgacattt taagatcctc tttctgcggt tacatcacca cgacgagcat
     1021 cttcgcgggc ctttcgtctt gggcctccat ggcgtgcagg aaggacgaat cgaacaatat
     1081 cgagtccccc tcgttgagga cgatctcgtg atcgtggatc gagaacttga gggacccctc
     1141 caggacgtag tcgaactcct ggccgggatg gctgtaggct gccggtttct ccctcttcgg
     1201 ctcgatggtg acgatgaaga actcccccct cttggagagg aacttccccg ccaggttctg
     1261 gtacctgtac tgcctccgcc gctccacctc gaccccctcg cccttcctag taaccgtgaa
     1321 tatccgcatc ctcggctctt ccccggtgag gaggaggttc atgtcgacct cgagccgctg
     1381 gccgatctcg atcagcttgc tcgcggggat ctccatcttc ccctcctcgt agcagttgta
     1441 cgtctccagg ggcaccttca ggtgctctgc catctcctcc gccgtcacct tggacagctc
     1501 tcgcatctcc cgaattcggg ttgctatctc ctcaatggtc tccgccaagc cgattcaccc
     1561 actcaccctc gatatgccaa aatggtataa agcctccgcc tctctgccgg gggaaaaatt
     1621 cgataatatg cggtcgataa aaatctatcg ggggatctct tgccctttcc gggcctcgag
     1681 agggggacct caacctatta ataaaccccc caccccttga tcatatcgag atggaacccg
     1741 ttgaatatct tgagcacacc gccgacgtga agttccgggt ccggggccgg agcctggagg
     1801 agctcttcga gagcgcaggc ctcgcgctga tcggagccat gatagacccc tccaccgtcc
     1861 gggtcgagga gtcctggccg gtggagatcg aggcggagtc cctggaggcc ctcctctacg
     1921 agtggctatc ggagatcctt tacctattcg aggtggagct ggcggcgttc tcggagttcg
     1981 aggtcgccct cgagaggcgg ggggacggct ggagcctttc gggccgggtc gggggggaga
     2041 ggatcgaccg agagaggcac ctcttcgaga cggaggtgaa ggcggtcacc ctccaccagt
     2101 tcgagatcgg gagggaagag gtgggggggg aggagatctg gaccgctcag gtggttttag
     2161 acgtttgagg agatcccatg ctgaacagga tagacgatta catatttgag gtgccgatcg
     2221 gccaccagaa ggggatgcgc gtcccgggac ggatcttcat ctccgagagg ctcctcgagc
     2281 aggtggagtc cggaaccctc gaccaggtgg cgaacgtcgc caccctcccc gggatagtca
     2341 actactcgat ggcgatgccc gatgctcatc tcggctacgg ctttcccatc ggcggcgtcg
     2401 ccgccttccg ggaggatggg ggggtgataa gccccggggg tgtcggcttc gacataaact
     2461 gcggcgtccg gatgctcagg acggaccttc tggcggagga tgtggcgccg atgatgacga
     2521 acctcgtcga ggacctcttc cacgacgtcc cctccggcgt cggggcgaag gggaggctcc
     2581 gggtctcgga gcacgagctc gacgaggcct tcacccgggg ggcggcctgg gcggtggagg
     2641 cggggttcgg cgttcctgag gatctcgagc actgcgagga ggggggccgg atggaggggg
     2701 cgaggcctga ccaggtgagc ctgaaggcga ggaagagggg gcgtccccag ctcgggaccc
     2761 tggggagcgg aaaccacttc ctggagatcc agagggtcga cgagatcttc gatgacgacc
     2821 tcgccagaag gttcggcctc ttccggggcc agatcaccgt catgatccac tgcggatcga
     2881 ggggggcagg ccaccagatc tgcaccgacc acctccaggt cctcagccgg gcggtaaaaa
     2941 agtacggtat agagctcccc gacaagcagc tcgcctgcgc gccgttgggg acgccggagg
     3001 cggagaacta cttcggggcg atggcggcgg cggcaaacta cgcctgggcg aaccgtcaga
     3061 tcatctccgc ctggacgagg gaggtcttcg agaggtactt ccccgacgct aggctggacc
     3121 tcgtctacga cgtcgcccac aacgtcgcca aggtggagga gcacgaggtc gacggctcga
     3181 gggaaaggct ctacgtccac cggaaggggg cgacgagggc cttcggcccc gggaggcccg
     3241 agatcccaat ggcgtacagg gaggcgggcc agccggtgat catacccggg tcgatgggga
     3301 ccccctccta cgtcctctgc gggagcgccg gggcgatgcg gctcaccttc ggctccgcct
     3361 gccacggggc cggaaggatc atgagcagga cccaggcgaa gaagacctac gccggggcgg
     3421 aggtgaagga ggtcctgacg aggcgcggga tcaaggtgat ggcgactcac ccgaagatgc
     3481 tcgccgagga ggcccccgag gtctacaagc cgagcttcga ggtcgtcgac gtcgtccacc
     3541 acctggatat agcgaggaag gtggcgaagg tcgtccccct gggagtctcc aagggatgag
     3601 gatccgggtc gtcctggtgg agcctctcta cgaggggaac gtcggctccg tggcgagggc
     3661 gatgaagaac ttcggcttct ccgacctcgt cctcgtccgc ccctgcgaga tcgaggattt
     3721 cggcctcgcc atggcgtccc atgcgaggga ggtgatcgag ggggcgaggg tcgtcgatgg
     3781 tcttggggac gcgatctcgg ccgcaaacct cgtcgtcggg acgacggggg tgaggggtcg
     3841 gaccaccgac cgccacctga gggttccaag cctctccccg aaggaattgg cagagaggct
     3901 ccagggcgga aggggggagg tggccctcct cctggggagg gaggacgacg gcctctcctg
     3961 cgaggagctc gcagcctgcg atgtcgtcgt ctcgataccc acaagccccg agtaccccat
     4021 aatgaacctc tcccacgctg ccgccgtcct cctctacgag ctctcccggg tcgagggggg
     4081 ggaggtggag ctggctcggc ccgagaacct cgcccgcctc ctagaccact tccgggccgc
     4141 cctggtggag tcggagtacc accagcacaa gctcgagaag acgatgctga tgctgaagag
     4201 gatcttcggg agggcgaggc tcaccgaccg ggaggtccag accctccgcg gcgtcgtccg
     4261 gaacctccgg tggggggctg tccaccgcgg aggcggctct gaagaatggt gttttggggc
     4321 gggcaccgag gtccgggaag atggcgagga cgagggggag ggggctgccg ggtccgacag
     4381 gagcggggtt caggagtagg gcgaggggtc ctgaccggag cttcttgaac cggcttgcgg
     4441 catgtggccg cccaaaaaaa aagcattatg gggggcgagg ctctgccccc ccagttaggt
     4501 atcagacggc cttattgaga tcaggcgttg actacatagg ttcccgcgat cttgtcgtgc
     4561 caaccctgct tcttcttgtc gatgaggatc cagaggtatc cgaggaggat caccgcggtc
     4621 gatatgatca tcccgatcca ccggaggaag ccctttccgt atcctatcgg ataggtcccg
     4681 tcggtcccta ccagcttgat cttcatcgcc atcatccccg gagtctggcc gtttccgaag
     4741 aagtaggtgt agtacccgac cgataccaga aagcccagga aggcgattat gccgcccagg
     4801 gtctcgccga cgttcatcga ggcgtagctg aggatcgatc cgatcacccc gacgatgacg
     4861 ctgtcgatca ggtagcctat gaacctcgac ccgacgctcg ccaggtcgag gggcagctcc
     4921 cccctctctc cggcagtttc cgtctccatc ttccagcacc tccatgaaat ttcttcggta
     4981 cctctttaca taacactcat ataaataatt tctcttaatt tggtcttagc tgtcgtttga
     5041 tcgtctcatc tctccattaa actgggcgtg gacgaggata tcgccggaga tggcccgcgg
     5101 atcggcgccc ccgtcgaagg gaggcgccgg ggtttcctgg agaccctccg tcactccttg
     5161 acgacgtagg tcccagcgat cttgtcgtgc cacccctgcc tcctcctgtc gatgaggatc
     5221 cagaggtatc cgaggaagag gacgacgcca gatatctcca tcccgaccca ccggagaaag
     5281 ccccggcgga ggcctatagg ctcgattccg tcggcccgga ccagcttgat ctccaggagc
     5341 ctcatccccg gcgtctgccc cgccccgaag aagtaggtgt agtatcctat gccgatcgcc
     5401 gccatcagga ggtatatcgg gggataatcc gatgggaggc cgctccaggc caaagaccaa
     5461 tcctccgccg ggaggtcgaa gaggaagacg agggcgatgt agatgatgct caggacgacc
     5521 aggtccatca ggtatgacgc catcctctgg ccgatgcctg ccaggtttgg caccacccct
     5581 tccttcttat cgtatggttc attcattctt ctaacccctt ccttcgggat caaactccag
     5641 caatgtattt catgcaggta taaatattcg acttgctgag aaataagaga tcacgccgat
     5701 tcaaatcatg cagatgagaa agaaagggaa gaaggaaggg ggacggaatg gatttgaaag
     5761 gtgagggaaa aggcgatata tacgcaccgg gaggtcatac ctcccaggcg gcgatcacct
     5821 tctcgatgtt cacgtagtct cttcttcctc cgttctccag gaccaggacg ccgtcggcgg
     5881 agcccaccac cctcccgcga agccgctggt cgccgtcccc gccgaagtag acctcaatat
     5941 ctttgttgta caggtgctcg gatatgaatt tgtccatcat cgattcacca ccttaaagga
     6001 tctatttagc aataataagg ctttaaacta gatataactt gtggttaagc caaaaattcg
     6061 accagaaatc ccaaaaaaca cgccaaaatt taaatcaact tgattaaatg attttaactc
     6121 ggatcaactg gggttgatga tccgaagatc tttcctcaag tcaagtcgag tttaaatgga
     6181 aggcccagtt cgaaaagtgc taaaaggaga gatgcacggt tgagggcaaa aatctgatca
     6241 gcgccctcgc attttcccgt gaacttgatg gaggtatcat ccggatacct tgggcctcgc
     6301 cgcccccgag aaggtccccg aatccctcgg ctccgccctc ttttccctct ttatggcgtc
     6361 ctcgacggcc tcgcggatga tgtatcccgc cctgtgtagg atggtgtctt tcagctctgg
     6421 gtcctgctcc atgctggcgg tgcatgggta tgcgtggtgg gcctcggata tcctctcccg
     6481 gaggtcgggc tggtcggtct cgagtcccgt catcctcttt gccacgtacc aggtggatcc
     6541 gcacggggcg ctgcggacga cgttggcggc gaggatagtc tcctttcccg gccggctctc
     6601 ctgagctacg atctcgatct cgggctcgcc gaagtgggcc ttctgcatga aggcgtttat
     6661 ggcaggggtc gccgggttgg gcctgagggc gcagaagggc ttggcgaagg cggcttcgag
     6721 gcccagcctt ctcgcctcct cgatgagctg ggtccggagg cccaggggga ggtccttggg
     6781 catctcagac cctccaatga tccctttgta ccctttttct ttcaggatgg ggaggaggcc
     6841 gaatatgatg tcggggtgga cgtggacgac gatggcgata tccgcctcgg ggatctcggc
     6901 ggggatgtag ggagatacgt cgtcgatgaa gtcttcgagg ccctcggggt cgggcatctc
     6961 gatgacggat acgaggtcct cggcgaggct gtacttgccg gccttgcaga gggtgcagcc
     7021 gtcgccgcag gatatgcaga accccgagaa gttgatcagg ttaccgatga gggttcgccc
     7081 gaactcgccg ttgtagagga ccacgacctt catg
//
LOCUS       DASM01000050           29354 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_40401, whole genome shotgun sequence.
ACCESSION   DASM01000050 DASM01000000
VERSION     DASM01000050.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 29354)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 29354)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29354
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_40401"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         5764 a         8903 c         8819 g         5866 t
ORIGIN      
        1 gtagacgcgt ggtattacaa aggtagtgct atcaaaaaaa tggatctcgg gaagataagt
       61 ttcagaaaaa ttggcaagta ctccagaaga gcaatgttag cttttgatga ggtcatccgc
      121 cttaatccca agaatacata tgcttggtat cacaaaggtg tcattcttta taatagtgga
      181 aagtacgaag gtgccatcca ggctttcgac gaagtaatca agctagatcg caagaattca
      241 gacgcttggt attacaaagg agtcagtctt cataatatag gcaaatgtga agatgccgtc
      301 caggctttcg acgaggtcat tcgacttaat cctgaaaata cggatgcttg gtaccacaaa
      361 gataaggctc tgaggaaact gtatttcaaa aaaatgagca aatccgataa agctattctg
      421 acgttcgata acgtcaacca gcttgatcct gtaacccatt ggtggcttga cgcataaaga
      481 atatgtgaga acgattttga caaactccta ctgtatttca tgtccgtgca cagagtgtga
      541 gtattctgct ggcccctcgt cttgagcctc cgagggatga atccctctta tcctcactgg
      601 acgtcgttcg agatcttcga agggctaccg aacgtccggc cctgtgagag ggaccgagcc
      661 aaatagtcgt ggccccagaa tcagttcaca cgaggcggaa gcctcttgat ccagtccgtc
      721 ctggagggac tcttcaacgc ccgtgccgcc cggcgagatc gggacagtat tttgtatcag
      781 gaccgacctc gttggagcat atggctcttc cggatgtccc aaaagacggc gccccctttc
      841 gcgcctccga ctcctgcggc gatttcagcg gggggttcat tgccgacgtc ctggaggcga
      901 agagcgaggc ggagctgggc ctgggaaggc gcttcgcctg cgtcgacgac ctgatcgcag
      961 atctcctcga agactgagac gattcccgag ggctttatcc ctcagggagg tcggcggcgg
     1021 cccgaagctc caggccgcca atagtcggcg ccgccggtaa aatttaggaa aatttaaaat
     1081 tctactttta tatttagatg ggctcacctg acgttctcga ttttttaatt gcctggagcc
     1141 tcaacccgaa gatctttatc cctccgaatc cttttactcg caccggatca tggagctggg
     1201 cttcgggaga gagaagatgg aaccacgtct gaatatcgtg actctggggg tcgaggatct
     1261 ggagagggcc gtttcctttt ataaggacgg cctcggctgg ccgctctcca gcatcagcgg
     1321 aggcgactat gccatattca agatcagcac cggaacggca ctggccctgt tcctccgccg
     1381 tctcctggcg gaggatgcct ctgttgaaga ccgcgggggg tttggaagga tcactctcgc
     1441 gcagaacgtt tcctccagag aggaggtcga cggggcgctc tcgcaggccc tttctgccgg
     1501 cgggaccctt ctcaagaggg catctgagac cgattgggga ggctattccg gatatttcgc
     1561 cgatcccgac ggccatccgt gggaggtggc atataacccg cacttcgaac ttcggcaggg
     1621 caaactggtg ctgcctgatt gaggggcaga tccgctatga accgcaaaaa atacactcga
     1681 ttggaagaga tccccaacgt tggccccgct atagcggagg atctgcgcct ggtcggggtc
     1741 tctcatcccc aggatctgat cgggcgagat ccctaccaga tgtacgagga gctatgctgc
     1801 aaaacaggag agcggcatga cccctgtgtc atcgacgtct tcatctccgc ggtcaggttc
     1861 atggagggcg agcccaaacg gccctggtgg gcttataccc cggagcggaa gcagaggctt
     1921 gccggcgggg attcatttgg tcaaacaaag cgctccggca gacggaaaga agaaggaagc
     1981 cgctgaccca ggccgctggg agtgatacgg aatgctctgg gggaagggcc tccccccatc
     2041 cctcgcctgg acggcgggaa cgatcttcac cgatccgaag aggttccgct tcaggagaga
     2101 ataggaggaa gacggaagcc ctgagcaggc cggagccgcc ggaaagtcct gctcgaaggg
     2161 cgccccctcc gaaggccgcg gcgggatcct ggttctgcct ggaaggccgc ccatggagtt
     2221 ccgataccgt cttatcgcca ggcgacgagg gaggggcgat gaaccccgat agaatcgaag
     2281 agctcatagc cttccacggg gcgagcaagg aggagatcgc tggccgcctc gccgacttcc
     2341 ggcggatctg ggccgccggg tcggacgagg acctcttcgc ggagctcgcc ttctgcctgc
     2401 tgacgcccca gtcccgggcc agggtctgct ggcccgccgt cgagcggctc cgggggtgcg
     2461 gcctcctcta ttgcggcagc gcttgcgagg tggtggggca gctggaacgc gtccggttcg
     2521 cggagacgaa ggcggggcgg atcgtcgccg cccgccgtca gttcacccga aacggtcggc
     2581 tggccgttag gacggtcctg gaggggttcg cagacccccg agccgcccgg gagtggctcg
     2641 tcggaaacgt tttgggtttg ggctacaagg aggcgggcca ctttctgcgg aacatcggcc
     2701 ttgcggagga tctcgccatc ctcgaccggc acatcctgaa gaatctggtc ttgttgggcg
     2761 tcatcgacga gatcccgaag tccctcacca ggaagaggta cctggagatc gagaggagga
     2821 tgatggagtt ctcggtaagg acggggatac ccatgggcca cctcgacctc ctcctctggt
     2881 cgaaggagac gggggagatc ttcaaatgaa atcctgaagc tttgcgctgc cgctaccctt
     2941 ttcggcatca aggcccgcag acctgagggc ccgccgggat atccgcccgc cgtacctcct
     3001 cccgcccgcc ttccggggct cgtcctggct ctggtagtag acggtccaga ggtcggcggc
     3061 cgccgtcccg gccccctcct cagcccctat atgctgcggc tccaccttcg tttcgtcttt
     3121 atcttttcct cccttctcct ccgagacctg gcggccctcc tcctcctcgc ccccctgggc
     3181 caggagatcc atgatccccg atatctccgc cagggtggcg gcgaggccgc atccctcctg
     3241 gtggaggaga gctccgtcgg aatagaggga ggtccaggac atcctcccgt ccccgaggac
     3301 gataagcttt cccggaaacc gcctcgcgaa gaagtcagag atctcaaacc cgacgtcgtg
     3361 atggggggag atcctccccc agaggacccg gtcccccagg ggggcgagcc tgataaaccc
     3421 cctcatcctg cagacctcgc cccggacggc gagggccatc ctccggatcc tgaggacctc
     3481 cgggtccgtc gagccctcca cctcctccag cgatagcgtc ctcgcccggg ccatcagccg
     3541 ggcgttcgag tcgcggtggg cggcgagccg ccggagaagc tcttccgtcc tcatcagaac
     3601 ccccaccggt ccagggtcgt cgccgatttg ccgccgacct ggaggaaggg gccggcccgc
     3661 cggggggcga cgcccagctt ggccagatcc ctcatccgct cgatccgtcc attttttcga
     3721 cggaggagga tcctccgcgc ggttttgggg ccgatccccg ggaccctgat caattcggcg
     3781 aaggaggcgc cgttcgggtc gatcggctcc tctaggctcg cccgggcgat cgccaccttc
     3841 ggatcgccgt cggggagata gcccaagtcg tcgaaggcag ccgttatctc ctctctttcc
     3901 agcttataga ctcgatagag ccaatcgacc tggtagaggc ggtgctccct ccaggcgggg
     3961 gccctctccc tatcagcgaa ggcggtcccc gcaagagggg tgaaggcgga gaagtagacc
     4021 cgcttcaggt tcatgacgtc gtactcgatg agcatccggt cgaagatctc ccggtcggtc
     4081 tctccggccg cccccaccac catctgggtc gtctggccgg cgggcaaggc gacatctcgc
     4141 gacaggtccc ggatgtacct ctgcctcgcc aggatgtcat tttcgtactc cttggaaggg
     4201 cagagctccg ccatcctcgc cgccgacggc ccctccaggt tcaccgagac ccggtcggag
     4261 agctccatcg cctgtcggac gtgctccttg gaggaaccgg ggaggatctt gaggtggacg
     4321 tatccccgga aatggtgggc cgtccgcagc agccggaccg cctcgatcat ctcctccatc
     4381 gtttcgtttt cgtccctccc gatcccggag ctgaggaaga gcccctcgat cctgttctct
     4441 tgatagaggt cgagggtgat ccgggcgatc tcttccggcg aataggcgaa ggtcttcttt
     4501 tttgcggagg tggaggcgtt ggggcagtag ccgcagtcgt gggggcagnn gtgaaggagt
     4561 ggcagagccc tccccgcaac gccgaggcgg cgtcgtcctc tctccccctc ctcgccgccg
     4621 aggaggtgca gaggtcgtac ttcgtccccc tccccagggc ctccaccttg gcgagatcga
     4681 gggcgcggcc ggaggcaggg ccgacggagt cgaagctgcc ccctgcggcg aggcgggcca
     4741 tcttctccag ggttgtcacc ttcggatgat cgcctttcct tcagatagga tcatgggtag
     4801 cggggggcga aagtatcctc tctcgggttt gaccgccact ttcagtaaca aaaaagttta
     4861 tagaaacctc tcatgagatg gcgtcatttt taatgggggg ggatatccgg agatggagca
     4921 aaaaatagat actatcgttc tctcttatcc tttaaatagt ccttcatggt ttgaagatct
     4981 cagatatgaa tttctaatat aaatataata gtttggtttg atcctgccgg agatatatcc
     5041 aactaatcga aggagatata tctcttgcag gtacaaaata gaaagatgtt atatctaagg
     5101 tgatggccat gaccaaggaa gataagacat acacctaccg ggcgggaaag aaggtccccc
     5161 tcgagaagga ggaggaccag ttcgtagtcc gggcgctccc ggaggatctt gaagagatgg
     5221 ggatctccga tgccgagagg gtctcctctc gatcctcgaa ggtcacgacg agggccgggg
     5281 atctcgagca gctgatgagc atgaccagac acatcgcccc cacccaccac gcctaccggg
     5341 tcgcggagac gggggaggag tttctgatat cagaccggat catcgtcacc ttccgggagg
     5401 ccctcccggc tgaagatgtg gacgccttcg ccgggaagta cggcctgttg aggctcaagt
     5461 cctactccga ccgggaccac ctcttccagc tgaccgacgg gacggggatg aacccggtga
     5521 agctggtggt gaagctgatg gagcaggagc cgaccgtcga gttcgcggac cacgacctca
     5581 accggcggat ggccacctac gatttcgaga tcccgaggga ccctgcctac tccctccagt
     5641 ggcacctcca cacccgtttc tccgggagga gcttcgatcc ccggtcgtcg tcgatgtgcg
     5701 aggacgcctg gcggatcctc gggaatttcg ggagcggaag cgtcgtcgtc ggggtcaccg
     5761 acgatggatg caggctcgat catgccgact tcgactcccc cgagaagttc gccggatggg
     5821 gttacttcag ggggacgacc ctcgtcaaaa agatcaacat cggtgccgac ccctccgaga
     5881 tgtacacccg gggcgacgac cacgggacct cttgctgcgg ggtgatcgcc gccgaggcgg
     5941 actcggccct gacggtggga gccgcccccg cctgccgcct cctcccgata aaatgggagg
     6001 tggacgactc ggggtacctg ctcatcagcg acgacaaggt catgacggcc ctgagctacc
     6061 tcgccgacaa ggtggacgtc ctctccaact cctgggggtc gagccccacc ctcgacttcg
     6121 cccccgctgt aatccggcgg atcggcgacc tctccaggac cggggggcgc cgggggcagg
     6181 gtatcgtctt cgtatgggcc gcgggtaacg ccaactgccc cgtaaatcac actggctcga
     6241 tggaggtccc cttcaccgac ggctgggtga tcaggcccga cggaagcccc cactgggtgg
     6301 gggtcgatac cgcccgcagg ttcgagaacc agctcgtcgg catccccggg gtgatgatcg
     6361 tcgccgcctt atcgagcatg gcccagagga gccactactc caattacggc agcggtatct
     6421 ccatcactgc ccccacgaac aacctccact cctactacag gctgaccctc ccggggctcg
     6481 ggatcaccac cgcctccggc gagcgtatcc cggtgacgga agagttcggc ggtacctcca
     6541 gcgccgcccc cctggtggcg gggatagccg ccctggtcat ctcggcgaac cccgacctgt
     6601 cggccctgga ggtggtatcg atcctgaaga ggaccgcctc gaaggacctg aacctcggcg
     6661 ggtactcccg gacgcccccc gccagctttg accccgatac gagctgggac gtctcgccga
     6721 tccccccctt cgaccgcggc gatttcgtcg acgtcggaga cccggccggg agctggagcc
     6781 cgtggttcgg ccacggccgg gtcgacgcgg cgagggcggt ggccgaggcc ctccgcctcc
     6841 ggggcgaggg gagggacaag gtcttcaagc aggcccgaac cccggacctc cccatccccg
     6901 acaacgaccc cgagggggtt agggattcga tctccttctc cgaggccgcc aacctctcca
     6961 gggtccgggt caccgtcgac atctcccatt ccttcatcgg agacctccgg ctgaccctca
     7021 tcgccccctc ggggaagttc gtcgtgatcc acgaaaggaa cggcggcggc tccgacgaca
     7081 tcagggcgac cttcgacccc atgacgaccc ccgccctcgc ctccctcgcc ggccagttcg
     7141 tccaggggga ctggaccctc ctcgtccagg acctggcagc tcgggatacg gggaggctca
     7201 accggtggga gctggagatc gagggggaga agggcgggtc cgtcaacctg gaggacgccc
     7261 cggcgtccac catccccgac aacgacccca gcgggatcgt ggcggcgatc agcaccaccg
     7321 accccgggag ggtgagggat ttggaggtct acgtcgacat cacccacggc ttcatcggcg
     7381 acctggaggt gaccctcatc tccccggcgg gaacggccgt ccccctccac cagcgggagg
     7441 ggatggccac agacaacatc atcaaaagct acacctccgc cacgaccccc gacctccaga
     7501 agctccgggg tcagcaggtc cggggagcct ggaggctgaa ggtcgccgac ctcgccaggt
     7561 ccgacgtcgg aaagctgaac cggtggggtt tgaggatagc gaaagaggcg gaggcctgat
     7621 ttgaaaccac cggcgtaggg gaccgccgga tcgacgagac tcggcttccc ggcagccgaa
     7681 gaccttcggc gggacagtag gcagatccgg accctaaccc ctccgaccgg acccttcctg
     7741 ggagagggct ccggcggagg caagggtcat cccgccggga aaagggctcg acttggggtc
     7801 tcgctcccct ggcctgatcc gccgctcgtg gggtgttgaa ggatgagatc agaggtaagc
     7861 gtcgacgggg aggggtggag gaagcggttt cccgagaagt tcggcccgat ggaggaggcg
     7921 tttggggacc tccggtccgg ggccaggatc ttcgtcggga cggcctgcgg agagccccag
     7981 gccctggtcc gggggctttt ggaccacctc cgggcctcat cgacggagct cttcgacctc
     8041 gagctcctca cgatcttctc cctcggatcg gccccctatg ccgaggaggg gttgaaagag
     8101 agcctccgga tcaactcttt cgccatcgga gaggcggaga gggattccgt caacgacggc
     8161 tccgcggact atacccccgc tttcctctcc gccgtcccgg acctgatccg ccaggaggtg
     8221 atccccatcg acctcgccct cgtccagacc tctctccccg acaaagccgg gaacgtcagc
     8281 ctcgggatca gcgtcgacgc cgtcctggcc gccgtggagg agtcgaagat cgtggcagcc
     8341 caggcgaacc cccggatgcc ctacgtcctc ggcgagggga tcgtaaacct ccgggacctc
     8401 gactacgtcg tcgttcacga cgagcccctc ctcgagcacc attggcccct cccggagggg
     8461 gtggtgaaga ggataggtga gaacgtcgcc cggatcgttg aggacggggc caccctccag
     8521 gtgggcttcg gggggctgcc cgacgccgcc ctcgctcatc tatcggagaa gaggcatctg
     8581 gggctccaca gcgacctctt caccgatggg gcggcggagc tattgagttc gggagtgatc
     8641 gacaacagca gaaagagcct cgaccccggc gtggccgtcg cctccttcgc cgtcgggagg
     8701 agggagacct atgacctcct caataaaaat cctgccgtcc tcttcaggcc catcgaccgg
     8761 acggcgagcc tcgtcgtcat cgcgagcatg aggaatatga cggcgataaa caccgctctt
     8821 gagatcgacc tcaccggcca ggggacggcc gagtccctgg gggggaggtt ttattctggc
     8881 gtcggcgccg aggcggagtt cgtgcggggg gcggccctcg cccccggcgg aaggtcgatc
     8941 ctcgccctcc cctccctctc ccccgacggc agggcgagcc ggatcgtccc ccgcctcgcc
     9001 ccgggggcga gcgccacctt ccaccgggga gacgtccagt acgtggtgac cgagtacggc
     9061 atcgccaacc tccggggaag gagcgtgaga gagagggcga tgagcctgat cggcatatcc
     9121 catccgatgt tcaggccccg gcttatggaa gcggcccgga agctctccct cgtctatagg
     9181 gaccagatct atcgttcctc cgactacccc gaggatctgg aggcatggaa ggtgacgagg
     9241 tcggggcttt ggatcttcct caggcccgtc aagatcagcg atgagcctct agtcaaggag
     9301 ttcttctact ccctatccga taagaccctt tatcggaggt tcgcctcggc gcggagggag
     9361 atgcaccact cccggatcca ggatttcgtg gcggtggact actcccggga tatggtgatc
     9421 ctcgccctcc tcctccgggg ggatagggag gtcgtcatcg gcataggcca gtacagcagg
     9481 ggcggtaggg cggagctcgc cgagctggcg gtggtggtaa gagacgacta ccagcgccgg
     9541 ggggtgggga cgctcctcca cacctacttg acggatatcg ccaagaagag gggcctagtc
     9601 gggttcaccg ccgaggtgct ggaggataac ctcccggctc tgaagctgat aaagaagatg
     9661 ggctttgaga gggtggcgat ggacggcggg gccgaggaga tgaggatcgt cttcgatggg
     9721 gcgggacgtc gatgattccg gcgggtcaga ggaggccggg gtggatcttc gagtggaccc
     9781 tcgtcagctc ctccagggtc tcggcgagct cggcggagac cctcttctgg gtctctatgc
     9841 cggggctcat ctccaggtcc ggataggaga gggggacgta gccgacgacg ttggaaaaga
     9901 gctccaccca gtagggcggg agatactcgg ggaccggctc gcccctcatg atggccaggg
     9961 cagaggtcca ggcggtaggg accaccgcca ggttgtaccc cccgccgccg agggccagaa
    10021 gtctcccctt cgcgtaccta ttggtcagat cgacgatccc gcggacagcg gtgacgtacc
    10081 cccggagggt gacgttgagg ctcgtcagcg ggtcggagta gtgggtgtcc acccccagct
    10141 gggccaccac cgcctccggc cggaaccagt caaagagctt gggtcccacc tcctcgaagg
    10201 caagaaggaa gtcccggtcg ccggcgttca tcggcatcgg gacgttgaag gagtagccga
    10261 gccccggccc cgcccccgcc tcgtcgacga agcccgtccc aggaaatagg tatttgccgg
    10321 actcgtggat cgagaaggtc agcacctccg ggtcctcgta gaagatctgc tggaccccgt
    10381 cgccgtgatg ggcgtcgatg tcgatgtaga ggacccggga gaacctcctc ttcaggaccg
    10441 agatggcgag ggcggcgtcg ttgaagacgc agaagcccgc agcctgggtg gggaaggcgt
    10501 gatggaggcc gccggcgagg ttgaaggcgg cgcagtcctc ctccagaatc cttttggcgg
    10561 ccgcgatgct ccctccggcc atggagcatg acgcctcgaa gatcccggga aatacggggg
    10621 tatcctcgct cccgaggccg aaggcgagat ccgggtcctc ctttctcacc gcctcgaggt
    10681 actccagggt gtggacgagg gagagctcct cctcggtggc gggttccggc aacaggatct
    10741 ccgtcctctc gtcgatcagc cccgaatcct cgatcagctt atgggcaagg ctcaccctga
    10801 tggggctcag ggggtgctcc gggccgaagt tgtatttttg gaactggtcg gtgtaataaa
    10861 ggtagatcat aaggaccctc tcttgctatg gatcatggcc acataggggt gcgtctcctg
    10921 cggcgggcaa atgatttcga tctttattct atcctccggt ctgatgttgg ccaaatcatc
    10981 tcctcaaggg agagatgaag gcggaaattg tggtggatga aggccgattg ctttaaaaaa
    11041 tgaagcctct catatttcat taagttttcc tatctggatt ctgttccact ttagagtccg
    11101 ggttaggcca cctgtctgtg ctacaccctc gaagtcgtcc ccgtcgcctt aacgatagca
    11161 ctgcagatca tgaaaacgga gcgattccac cagtatctcc ggctgttctt ggccgcgctt
    11221 ttcagaattg agatagtagg ctttcctata tgtcctcgca cgatgctgaa tttgagacag
    11281 atgatgagcc ccagaagagg agcgtcgaag atctcgaaag ggatctcagc gggcggaggg
    11341 cgcttcttgc agaggtttac gataacgtag tcaagagccg caactactca acttcgttct
    11401 cccaccatat catggcgctt ttcatataat gctttgacca taaccctcca ccttcctcga
    11461 tcagattttc tgatagatag gacctgatga tatgctcatc cttctcagaa gatacgtcta
    11521 aattcttgcg ccaaaattcc gtagcatctt gcagatcatc aaatttctgt acattttcga
    11581 tattttctat gtatacattt gcataaattc ccatatcttg gaggaggtta tagatgtaaa
    11641 tgtagtccgg accgatgtcg tattcttcat cgtgcagcgc tggccaaatt tcgctgtagt
    11701 cccaataagg atctccgacg aatgtgaaca agcagatgcc tctacttgcg agctgtacca
    11761 ttttaaaaag tgcggcttta atatccagca tgtagagaga gtaagaagcg atcagaaagt
    11821 cgtgcacgtt agtgtcttca aagggcttta catcttccca ttttttattt atatagttta
    11881 tgttggtgat tccaaatttt tgagcgtttt tctttagatg ataaatcatc tccatcgaag
    11941 gttccaccgc ggttacgcaa cgggcgattt tgaaaggggt atcgcaagag ttccaggacc
    12001 cgcgcctata tccatgatcg tagatcccgg tcttattcca agctttgaga ccagcttacc
    12061 aacctgatta ttccttatct ctcttaacaa agcatctttg ttatactgtt ctgcccgttt
    12121 tcgggctgtc cgagggttca agtccttaca gcccttcatc caggtggctt cattcatcgc
    12181 ctccatccac attccgttcc aatccaagtc caccatctta ccctacctcc cctttaaacc
    12241 aggattgtgc aggggtgttt catccttcag ttcagtcctt ctcccctgca gattcctggc
    12301 ttacagatgt ttttcatcca ttttcggtct gataaataaa cggagggctt caagtggaag
    12361 gatatacaaa agttccaagt tcgctcagat cgtagtccag gcccatgaat ctcgtaaggt
    12421 attcctgatg gaccgactcg atatcttcaa cgctgatgtc gctgaaaagc tctggataga
    12481 accacttagc catgtaactt attcctacga tatgttttcc tccaccacag gtgacgtccc
    12541 atggaattac gtagactcta ccatctttta cagccgttac gttctgaagc tcggccctgt
    12601 tcatcatctc gtcacggatc tctttcatct tttcgggatt gccgccaaga ccactacctg
    12661 tttcagcata atccccgtac ccataattta gtatcacatc aggatttcgg atcatcacag
    12721 cctcggggtc tacgtcgaag aagctttgat tgtgatcgcc gaatatgtta attccgcctg
    12781 ctgtgacgat gcattcgtgc aatccaaagg acccctcact acaggtactg tagccttcag
    12841 gccaggtagc catctcgaaa tagactttcg ttcgatcctc ttctggcgcg gtcgctacac
    12901 gttcttttat ctcgtccaac catccgttat agaactcgat gaactcttca gcttccgctt
    12961 ctttgtctag gatgtatccc agttgcctca attcccctac gtaactttcg attcctacga
    13021 aatctgctcc aattacggta atttcgggat ctgctttatt caacgtctct cgaatgtttt
    13081 cgcatcttgt ttgatggacg gggacatttg caaacaagaa tactacatct ggatctaggc
    13141 taaggatctc ctcgtagtcg ggatcatcaa cgtagccgat atttgaagcc ccttcgatct
    13201 tgtagaaatc gtttttctca actatttgtc tgttgatgcc gactatttta tcagatgcac
    13261 caagggtttg gatcgcttca gtggttccat agtgaaagca aattatccta tcgaccggtt
    13321 ttttaaccgt aacgattctg tcggcgctgt cgactatcgt gatctcgctc tcttctcctt
    13381 ttataatctg ctcgatctgg gtgatatctt tctcggtgac ttcgccatcg taattcgcgt
    13441 cggagaactc agtcctttcg ttggtcctct ttatgacgcc ctctacatat gctatatcat
    13501 cctcgtcgat cttctcgtcc atatttgcgt ttccgaatac ttcaagcaaa tcattcgagg
    13561 caattgcggg tgtcgttaca aggatggtgc ataataccag cgaccgaaat atccacgaaa
    13621 taatatccat ttcatcgtct ccacaagtta tgtaaaatct aggtaaatat ataccataaa
    13681 tatttatttt ttatggttac tagatcttac tcaaatgtaa ataattagtt ttatgttata
    13741 agctttgcgg tatttatatc ataaatatct cttttaattt gatattatat catttattca
    13801 tacttattag caaaagttta tatacgtatc tggttaataa gccgcggcac cgcaactatg
    13861 gaggatgctt aattggtcat aaaacgattg gatacgaggt caaagatctc ttattgcgcg
    13921 gttttgatgg cattgatcag tgtcgtcttc ttcacttcct tcgccggact ggcagaggaa
    13981 acgattacca tcgtcgattc agcggggaga actgtagaat taacctatcc tgtgaagagc
    14041 gtcgtggccc tgaacgggaa cgtgccagaa gagatgatcg ccctcggtgc cggggataaa
    14101 atcgtgggca taggtatcgg aacaaagacc aaagtcgacc agggtctact tcctgggctg
    14161 gaggatgtgg ccgtcatcgg agatgaggcg gaccccaact acgaaaagat tgcagaactc
    14221 gacccagatg tggtgataca gtacgcctcc tggcctccga cgcctgaaga gcttcaggcg
    14281 aaactggacc cctttggaat acctgtcgtt gccctggacc tctacaggat ggagatctac
    14341 tacgatgagg tggagcttct tggcaggatt ctggggcatg aaaaggaagc agcggattat
    14401 atcgccttca ttcaagatca gtacgatctc atcgacgaga ggcttgagtc gatccccgaa
    14461 gaggatcgaa aggtggtata tttcgagggt gctcgccttt accaaaacta cggaggcgct
    14521 ggttttggag ccggcatacc cgggatgatc atggccggcg gcggcaagga tctctacccg
    14581 gacatcaaat ctgatgtctt cgatacaaat ccagaggacg tggcagagag gaatcccgac
    14641 gtaatcttca aggggatctc agagcccaaa ggctacttca tggagaacga aaccgttctt
    14701 aagaacctga gggaagaagt gataagcagg cccatgtggt ccgatacaac tgcagtgaag
    14761 aacggcaaag tctatgctat aagcttcgac gccacggcag gtctccgaaa gaagtttggg
    14821 cctctattca tagccaaagc cctctatcct gagaccttct ccgacttgga cccagagtct
    14881 ttcctcatga cgtatctgga agactatctg ggactagagc cccagggctt ttacatatac
    14941 cctcccctat gaattttccc ataatatttt tttaatcata aggagttgaa tctgatggta
    15001 gaaacggatt acatacttgc ggactgggaa aaggcggagg gcgtagcgac atccgaggga
    15061 tttataaagc cgctcatagc caccaagaac gtatcagtaa cggctttgga gatcgctcca
    15121 ggggacgaag ttctccccca cagtcacagt ggcctccctt attttgaggt ggttctatac
    15181 gtcgtcgagg gggatctgga gatcatcttc cgggacaaac gcgttccagt aaaaacgggc
    15241 atggcaataa tggccgatcc gaacgagcac ggctggttca acggaactga agagacggta
    15301 aaggttctga tgatccactc tccacctcca tcatggcaat ctcccgagga gttcctcgaa
    15361 aaggttcgca ggtaccaaga tcgaaaatga tcggatatct gagctgatga tcatcgggtc
    15421 tagggataaa aataaaattt gaggagggag gatcggtgaa taccacagac aacacaagac
    15481 agctatacat gaaatacatc gccaggaaga tgcttttcat agccgtctct ctcacgatcc
    15541 tcttttttct cgtcttgctg tccgtctcaa taggcacatc catcgagagc ttaaaggacc
    15601 tgcaaagcct gaaagatgtt ctaaccgcaa tagtcggccg tttcttcctc caggtggaga
    15661 cgaatccctt catggagaag gtgatctgga attacagact gcctcgagcc ttgttggggg
    15721 tggtggcagg tgcaggtctt gcggtatccg gcgccgttat gcagggagtg acccgcaacc
    15781 ccctggtcag ccccttcacc ataggcattt cctcggctgc tgccttcggg gcatctatcg
    15841 ccattatttt aggggcagga tttgtcggga tcggcaccta ccttataatg atcaacgcct
    15901 tcatctttgc cctagtctgc gctgtcatag tcttcggcct cgccaaatta aggggcgcat
    15961 ctcctgagac ggtagtcctg gcgggaatcg ccctaacata ctttttcggg tccgccaccg
    16021 ctcttcttca ctatttcgct tcgcaggaac agctcagcgc catggtcagg tggacatttg
    16081 gcaccctctc cggggccact tggggaggga tcggaattat tacggccgtg cttgttataa
    16141 ccatacccat gatgatgaag tactcatggg atctgaacgc caccttctcg ggtggggacg
    16201 agaccgcaaa aagcctggga atcaacgtat ccagggtcag aaccgtctct cttatcctct
    16261 ccgccttcgt taccgccacc ataatcagct tcaccggaat tataggtttt gtgggcctgg
    16321 tagcgcccca catatcccgg tttctgatcg gaggagacca caggtttctg atccctgcat
    16381 cgggcatcat gggatcaata cttgttgtgg gaggggacat agctggcagg attgtaagcc
    16441 cgccgataat gtttcctatc gggatagtga tatctttcgt gggggtgccg atgttcctat
    16501 acctgatgat gacgaaaaga aagagatact gggaatgact tgtttcaacg atatgattat
    16561 tggcacttgt tttatccgcc tctcgcaagc ctcctgatca tatcccaccc cgtcccccgc
    16621 cccaggggct ggtcgaggag atagtcgagg aactcgaaga cgatcggaga gatgaagacg
    16681 taaaactccc gggtgaaggc gagggcgtcc tcgacgcttc tgaagtggcg gacctctatc
    16741 gtctgacccg aggcggtctt ctccggccgg acgacctggt cggcgacgaa ggcggaggcg
    16801 ccgcaggagg cccgggcggg ctggagggcg tcgatgttga tgggaacgcc gcgatagtgg
    16861 cggtatacgt cgaatatgag ccgctcgcgg ctggggctct cctcctcgga gacctccagg
    16921 acgacctcgt cgtcgagggc ctccggccgg gggacgatcg ccgcctccac gacgaaggga
    16981 tactcctctt cggggccgag agaggcggtg aggtccaccg cctcaaagac gatcccaaga
    17041 cccaccctcg atccgtcctc cagctcgcac cgccagccgt catcttcagc gtagagggtc
    17101 cttttgtcga gagtgaggag atggacggag ccctcggcta tcagcgtcat cagaggacct
    17161 ccacgtccgc ggccctctgg accgagatct cccggcgccc ctcataccag gcgatctccc
    17221 ccttcgcccg gatgcggtct cctttggcga tccttccggc caggagctcg gcgccggagt
    17281 cctggctcac gaatatcggc atctgggagg agtagagcct gattatgagg tggccgcctt
    17341 cggtcctctg gaccgaggcc gccgtcccct cggcgacgat ggccaccttc tcgggagcgg
    17401 gatcgaagtc ccccagcccc caggaggcgg cggcgaggga tccgagggcc atcaagagca
    17461 acaccaccac caccttctcc tccttctgca tcaggtcacc ctgccttcgg cccgaggtta
    17521 gcgcccccag gatagacggc ggaaaaaaag gataataccg ccgtggagcc ttcagggcct
    17581 tacttcttcg cctctttctt ctcgccgaag tgctgctgat aggccgcctc gcaggaggcg
    17641 aggcagtcct gacagagccc tttgtacgcc ccttcggggt ttgcgagggt atgcacctga
    17701 actttcaccg gcaccagggt tggcttagca agtccacaaa gttcacaatc tgccatgtta
    17761 caacatctcc cggggtcccc cataaacctt tctcccggtt gaacttttcc atcagcccag
    17821 aggcgtggaa gccgcaccga tggggatcta cctctcgggg aagggagaac gccttaaacg
    17881 ggtaggggca agaggaggac catgagctgc tatccaaaga gaccgatcat caccctcctc
    17941 tcggacttcg ggtctctata tccggcggag atgaaggggg cgatcctctc ccggatcggg
    18001 gacgcgatcc tcgtcgacat cgcccacgac gtgccgcccc aggacgtccg ggccggggcc
    18061 ttcgccctga tggccgccgc ccggcacttt cccaagggaa cggtccacct cgccgtcgtc
    18121 gaccccggcg tcggcaccga ccggctcggg atcgtcgtgg cgagcggagg ccacctattc
    18181 gtcggccccg acaacggcct cctcctcccc gcggcaaaat ccgcggggga gcctgcggcc
    18241 tggatgatcg gatcctcctt cttcgagggg gcggccccca ccttcgccgg ccgagacgtc
    18301 ttcgccccgg cggcggcggt cctcgcctcg ggcggagacc ccgggtcctt cggaccggcg
    18361 gtggatccgg cgggcctcga cttcgggagg gcccggatcc tggatgaggg gatcgaggcg
    18421 gaggtgatat acgtcgacgg attcggaaac ctcgtcctca actcggccgc gatcccctgg
    18481 gaccgggtcg agctaatggg gagacgcctc cgccgggcga ggacgtacgc cgaggcgggg
    18541 gccgacgaac cccttataac ccttgggtcc catgggtttg cagaaattgc ggtcaacggg
    18601 gggagcgcaa aggatctatt cggcctcgcc ccaggcgacc ggatactctt ggagagagga
    18661 gattgttcgg aatagagttc gtcccaaatg agcccgccct gaaggtgggc tacatggcaa
    18721 agctggcgga agatgcgggc ttcgacacca tctggatcac cgatcactac aacaaccgag
    18781 acgtctactc gaccctcgcc atcctcgccc tcgccacgag cagggtcagg ctcggcaccg
    18841 gggtcaccaa cccctacact aggaacgtcg ccgtcaccgc ctccagcatc gcctccatca
    18901 acgagatctc cggcgggagg gcgatcctgg ggatcggccc cggggacaag gccaccttcg
    18961 acgccatggg gatcgcctgg gagaagcccc tctccgccgt cagggattcg gtggcggcgc
    19021 tgcgggagct cctcgcgaag aagagggtgg aggggcacgg gatggcggga gcccagctcg
    19081 ccttctccgc cgggaatatc ccgatataca tcggggccca ggggccgaag atgctggaga
    19141 tggcggggat gatctccgac ggggtcctgg tgaacgcgag ccaccccgac gacttcaagt
    19201 acgccgtccc cctggtgagg cagggggcgg agaaggcggg gcgggacccc aaagaggtgg
    19261 agatctccgc ctacaccagc ttcagcgccg acaagaaccc cgataaggcg gcgaacgagg
    19321 cgaagatcgt cgtcgccttc atcgtcgccg gctcccccga gacggtcctc tcccgccacg
    19381 gcatagaggc cgaggaggcg gcggccgtct cccgggccat cgccaaaggc gacttcggct
    19441 cggccctcgc cgccgtcacc agcaccatga tagacgtctt ctccgtatcg ggaacccccg
    19501 ccgactgcag ggcgcgggtc gacgcgctcc tggcgacggg ggtgacccag atcgtcgtcg
    19561 gatcgccgat agggccgaac aaggagaggt cgataaagct gatcggaaaa gagatcctat
    19621 gaatagaggc ctgtacatag gacggtttca gccctaccac ctcggccacc aggccgtcct
    19681 ccagaagatc gtcgaggagg tggacgaggt cgtagtcggc ctcgggtctg cccaggcaag
    19741 ccacacaatc gagaacccct tcaccgccgg ggagaggatc agcatgatct ggcccgccct
    19801 ccgggggctg gacgcgacct gttacgtcat acccctggtg gacatggaga ggaacgccgt
    19861 ctgggtcgct cacgtcgagg cgatgacccc gaagttcgac gtcgtctact ccaacaaccc
    19921 cctcgtcgtc cagctcttcg tcgaggcggg gatggaggtg agaaggccgc cgatgtacag
    19981 gagggaggtc tactccggga ccgccatccg gaggatgatc ctcgccggag acgaggggtg
    20041 gaggaggctc gtccccccct ccgtcgcctc ggcgatcgag gagatcgggg ggatcgatcg
    20101 gctgaagaac gtcagccggt ccgaccagga ttaggccctg ctccagcccc cagatcacgc
    20161 caacaggatc tacttttaac gattcatacg gccctatggg cctggaccga cctccggccc
    20221 cggcccctcc aggaccgtgc ccgccatggc tgccgattcg ttcgcctcgg gaaggtcctc
    20281 ctggagatcc ggtccggttt cgggtccgtc cgagctgtcg ggattgatat cggaatcggc
    20341 cttggtgagg tttccggccg aagccagccc cacctccagc gccctctcgt agcattcgat
    20401 cgcctcttgg tgcctcccca gggcgctgag ggcgatcccc ttgttgttcc atgcctccga
    20461 aagctctggg tcgagctcca gagcccgatc gtaggaggag atcgcctcct ggtaccggcc
    20521 gagggcgctg aaggcgaccc cctggttgta ccacgcctgg gagtaggcgg ggtcgatctc
    20581 gagggcccgc tggtaggact gtagcgcctc ctcctgcctc cccagggatc ccagggcgat
    20641 cccccggttg ttccacgcct gggcgtaacc cggatcgagc ttcagagcct catcgtagct
    20701 ggccgccgcc tcctcttgcc ttccgaggag gccgagggcg accccccggt tgtaccaggc
    20761 cggggggtag ctcggatcga ccttcaggac ggcgtcgtag cattcgaccg cctcctcctg
    20821 ccgtccgagg agcccgaggg cgatcccctt gttgtagagg gccatgacgt gggagggctc
    20881 gatcttcagg gcctcctcgt aacagtcgag ggcctcctgg tggcgtccca aagtcccgag
    20941 ggcgatcccc ttattattcc acgcggtggc gtagagggga tcgatcttga gggcctcatc
    21001 gtaaccctcg atggaccttt cgtactcgcc cgcggcgaag agggacctcc ccaggttgtt
    21061 ccagacctcc cggctccggg ggtctatctc gagggccctc tcatagcact cgatggagcg
    21121 gttgtaatct ccccggctgt agaagacccc gccctggttg ctccaggcga gggcataggc
    21181 cggatcgacc tccagggccc tatcgtagga ttcgagggcc tcgtctcccc tccccagggc
    21241 ctccaaaacc accccccggt tgttccaggc gggggcgtag tcggggtcta tctccaggga
    21301 gcgattgtag caggccaggg cctcttcgtt ccttcccagg gcgccgaggg cgatcccctt
    21361 gttgttccag gcgagggcgt actccgggtc gagctcgagg gcgttgtcgt aggaagcgac
    21421 ggcttcctcg tatcttccca gctccgagag agcgacgccc ttgttgtacc aggccagagg
    21481 atatagcgga ttctcctccg catacccctc ccccacggca ggcacaatgc agaccaaaaa
    21541 tagcgcgaag agcgcgaaga ggcgaagctt catgcttcgc ccttttattg tgtgagttga
    21601 tatggattac ggtcccgggc gaccgatcag ccctcctgcc gtctccggag agggccttcc
    21661 cagcggatca aactagacga gcatcctgcc gtgggagtcg atctctccgc cgcagatccg
    21721 ggtcgaggag atccggacgt cgtcctcggc cagctggtag tcgatcatca cgatcttgag
    21781 gggtgaaagg ccgttctccc ttctgatctc gttgatgttg caggccatag gctccgtctc
    21841 cggggagacg acgatgaagt cgtaatcctc ctcgatcgcc gagccgtaga aggtgtggag
    21901 catctcgatt tcgaagtcct ccctccccag gttctccttc agggcagccc tcaggttctg
    21961 gaccctcgta tcgaagttac ggaccggcct cgtcctctgg ctcttggcca tcccgtcgga
    22021 ggtgagggcg atcaccacct ccccgtcttc gcccgccacc tcgaaggcct tcctcaaaag
    22081 ggcgatatgg ccgtcgtgga tggggtcgaa ggtgccgcca actgcgactt ttggggccat
    22141 atggtcggca aagagggacg gtcggtataa gtaactggcg ctatggtgtg ggctggggcc
    22201 cctccgaccc cggggtcgat gaaaggcggt gctccaggac cttggcgacg atcttccggg
    22261 agctgcagag ggggcagggg tcgtgctcct ccagcctcac cacccgggcc ccgatccccc
    22321 gggtggccag ctcctcctgg aggaggaggg cgtcgaagtg ctggtcgtag cccagggtga
    22381 tgacgtcggg cctcagctcc tcgatcggct ggaacatatc cctctcgctc cccaggaccg
    22441 ccctctccac catcttcagg gccgatacca tcaggagcct ctgctcctgg gggatgatcg
    22501 gcctcggctt gtgcaggacg ttggcgtccc gggccacgat caccaccagc tcgtcgccga
    22561 gggccttcga ctgctccagg tacatcagat gccccggatg gaggaggtcg aaggtcccgg
    22621 tggcgagaac cctaatcaaa ccagccctcc tcgtcctcat cgatctccac gtccaggacg
    22681 gggaggtccc ggggagcgcc cgtcctgtca taacagatcc agctgtccat gtcgaagggc
    22741 atccccgcga tgatgtgata gtcgccggtc ttggagaaga agatcaggtc cgcctgggag
    22801 gggcggatgt cgggggaggg gtggctatgg acggagcctg ctatcctcat gttgggaagc
    22861 atgtaaagcc ggatgagggc gctcttggtc gacgcctccg tccccgggag catgatcacc
    22921 tcggtgatga cgccgtcctc ggcccggagg agccccgcaa actccaccgg gagggtagat
    22981 cgggacgcct ccaggatgaa gtagagcgtc tccctggcta tcccctccac ctccatcttt
    23041 ctcttcatct ccggacaccc cctcatctca aaaatatccc cgtacaaata acctccgctg
    23101 agaggaggta cctagatccg ttcccctgcg ggcctgaggt ggacgcagtc cccggcgatg
    23161 accctctcca gaaggccccc gtccctcctc accgcgagag acccgtccgc ctccagggcc
    23221 tctgccagcc cctcgaactc gccgtcccgg gcgaggatcc tcaccctccg ccccagggtg
    23281 gcggaccgga tcgaccactc ctcctggagc cggccggagg aggcctccat ctcctgggat
    23341 gccccctcca gctcctccag gaccctccgg acgagctccg ccctcaggac cttccttccg
    23401 agctccgagg atatcgacgt ggctttccac ccctcgggaa ggtcggcggg gtcgaggttg
    23461 gcgttgatcc cgatcccgac gacggcgtaa tccagcctgt ccatatccgc cccgatctcc
    23521 gtcaggatcc cggagacctt cctccccccc accatcaggt cgttgggcca cttgatcttc
    23581 acctcgaggc cgtagagccc ttccagggcc ctggcgacgg cgacggcggc ggcgatgttc
    23641 accctgaagg cctgggccgg agggacctgg ggcttgagga tgacgctcat ccagaccccg
    23701 ccccgggggg agtgccagga tctatccatc ctgcccctcc cagaggtctg gacctcggcg
    23761 atgacgacgg tcccctcctc ggaggcggcg atcctcttcg cctcggcgtt ggtggagggt
    23821 agggcctcgg cggcgatgat cctcctcccc aagagctccg tccttaggcc ttctcggatc
    23881 agatcctcgg tgaggaggtc ggggagggcc gagagcctgt agcccctctt tgaggcctcg
    23941 atctcgtaac cctcggcccg gagggaggcg acggccttcc agacggctgc ccggctgatc
    24001 cccagggaag ctgccagctc ctggccgctg atccaggacc cgaggcctcc gatcaggccc
    24061 ctcttcacct cctccctggt cacggcggga cctactcctc ggagccgggg ttttctcgct
    24121 gggacatgta ggccccgacg gctgcggata tggccgccac cttccgggtg tcggcgccga
    24181 gggcggaggc gagggagagc cccttctccc gctccgcctc cgtcaccttc ttcacctcct
    24241 ccatgatcgc ctcttccttg aggaagttgg tgttgatgtc cccccgcctg aacctctcgt
    24301 gccggaggac cgccaggtgg aagtttatgt tcgtcttgac gccggtgacg atgtactcgt
    24361 agagggcccg ctccatcctc gctatcgcct cgttcctgtc cctccccatg gccaccacct
    24421 tcgatatcat cgagtcgtag tagggcggga tcaggaaccc ggcgtaaact ccgctgtcga
    24481 cccttatccc ggggccgccg gggctccgat acctcttgat cctccccggg gatggggcga
    24541 agtcgttgag ggggtcctcg gcgttgaccc ggcactcgat cgcccagccc cggatctcga
    24601 tctcgtcctg ggagtaggcc aggggctcgc cggcggcgat ccggatctgc tccttcgcca
    24661 ggtcgacgct ggtcaccatc tcggtgatgg ggtgctcgac ctggagcctg gtgttcatct
    24721 ccaggaagta gtagtctccc ttcgagtaca tgaactccac agtccccgcg gaccggtagt
    24781 ctatcgcctc cgccgcccgg acggcgatcg atcccatctc ctcccggagc tccggggtca
    24841 tgataggcga gggcgactcc tcgatcagct tctggtggcg cctctggatc gagcactccc
    24901 ggtcggagac gtagatcgtc tttcccttgg agtcggcgag gatctgaaac tcgatgtgcc
    24961 tcggctcggt gaggtacttc tcgatgtaga cggtctcgtc gctgaaggcg gacctggcga
    25021 tcgaccgggc cgagtcgagg gcgggggcga ggtccttctc ctcccatacg atcttcatgc
    25081 cgatcccgcc gcctccggct gccggcttca gcatcaccgg gtagccgatc gcctccacca
    25141 ggtccatcgc ctcagccggg tcgctgatcc ccccctcgct ccccgggact atcggcacgc
    25201 cggcgctctt catgatgtct ctcgcggcga tcttgctccc cattctgtcg atggagctgc
    25261 tcgtcggccc gatgaagacg atcccattct cctcgcaggc ccgggcgaac ttcggattct
    25321 ctgagaggaa gccgtagcca ggatggatcg cctccgcccc ggtctccgcc gccgcctcga
    25381 ggatcctgtc gatccggagg tagctctggg aggctgggga ggggccgacg aagaccgctt
    25441 cgtcggcgta cttggcgaag agggcgttcc tgtcggcctc ggagtagacc gcgacggtgg
    25501 atatgccgag ctcgcggcaa gctctcatga tccttatggc gatctcgccg cggttggcga
    25561 tcagaatctt ctggaacatc gcccctgctt tggcggaccg aggctaaaaa cctttggcga
    25621 cccggaaagg agaagttaat aacctttcaa cccgagaggg ggggcatgaa gccgagggag
    25681 atgtccagag aggagtgcga ggagctcctc gccaaatcca ggttcgggag gatcggccta
    25741 tcgacggggg atcggcctta cgtcctcccc atgtcttacg tctacgccgg aggcgtcata
    25801 ctcctccatt cggggcttgc aggaaagaag ctcgatatgg cgagggagaa tccttttgtc
    25861 tccttcgagg tcgactccgt cgaggggggg aggtggagga gcgtcatcgc ctccgggagg
    25921 gtccgcctat cctccgacgc cggggcgaag gagaggctct ttgatctctt caccaggggg
    25981 gagatggggg gccacgccgg taaggccttc cgccgggagg atatggtgaa gatgccgatg
    26041 gtgatctggg agatcgagat cgaggagctg accggtcgcg agggcgtctg gtgaggggaa
    26101 ggccggcccc gggctgaaga gaggagtgga aggaccgaag gaaggatcga gggagaagga
    26161 cgggcaacta tcgcatgacc gccatcaagt ggacggagaa gtacaggccg aggaggctga
    26221 aggaggtcct cgggaacggg aaggccgtct cagagctgga ggcatgggcc cgctcctggg
    26281 agagggggat ccccgagaag agggccgtcg tcctctacgg ccccgccggg gtggggaaga
    26341 cctccgccgc cctcgccctc gccgaggaga tgggctggga ccagatcgag atgaacgcga
    26401 gcgacagcag gaccgccgcc gccatctcga aggtggcggg cgccgccgcg aggatgatga
    26461 ccttctccgg aaaaaaaagg ctggtcatcc tggacgaggc ggacaacctc tacggctctg
    26521 cggacagagg cggctccgcc gcgatgctgc ggctcgtaaa agaggccgac cagccggtgg
    26581 tcctgatcgc caacgactac tacggcctct ccaagcccct ccgggagggg accctgggga
    26641 tccagttccg gaggatcacc aagagcaccg tcctctcggc gctccgggag atatgccggg
    26701 cggagggggc ggcctgcacc ccggagctga tcgaggagat cgccgatatg gcgaacggcg
    26761 acctccggag cggcataaac gacctccagg ccgccctaga gggggcgagg gaggcaggcg
    26821 aggtggcgac gagcgtccgg gacgccaagg cctcgatctt cgacggggtg gccaagatcc
    26881 tgaggggctc gagcctcggg gaggccgtcc agatcgcgtg gtccctcgac gagagccccg
    26941 aggacctggt ccactggatc gacgagaacc tgcccctcgt ctacaagccc acagacctcg
    27001 ccgccggcta cgagaggctc tcccgggccg acgtcttcct cggccgggtc cggcggcgcc
    27061 agaactacgg cctctggcgg tacgcctcct tcatgatgac cggcggggtc caggccgccc
    27121 ggacggagaa gaaggggggc tacatcccct tccggccgcc cggctactgg cggaggatgg
    27181 gccagacgaa gggggcaagg caggtccggg agtcggcggc aaaaaagatc ggccgacact
    27241 gccacatcag ctcccggatg gccaaggggg agatgatggg gctcttcgcc ctcctcctca
    27301 aagatccgga tacggggccg aaggtggcgg ccctcctcga cttcaccctc gaggagatcg
    27361 ccctcctcct ggggacgaag ccgacgacca agaaggtctc gaggatccgc gaggcggccg
    27421 agaggatcct cgaggaggag agggtctcgg agattgaagg agcctggggc ggcgccaaaa
    27481 agccggagaa ggccgaaaag agggcgcggc ctcgaagggc ggataagagc aaatctgatg
    27541 ccgtcggcga aggggaatcc ggggcgggct cgaaggggag ggatctggat gaggagccgg
    27601 tggaggaggc cgagccggtg gaggatcgat cccccaagaa tggaggcgaa agctccgagg
    27661 tgacggagcc ggagaaggac gatcgccgcc agaggtccct cttcgacttc tgagcggcaa
    27721 gaatatcagg tggagtcctt ttgccacttt tgccacccca tccgagccga aagacgtcga
    27781 tgggagcaga tatgccgacc ctgagcttct tatcaccctt cctcgggcgc gatctcgtac
    27841 ctccagcacc catttctctc aacgcaaacc accgccctcg tgggagggcc agggctgatg
    27901 gccatgtcag taaatctgag atcctccggg gcgtcgggcc ttccatagct ttcccagctc
    27961 agccctccat cgctgctcga cagcaacccc acccggtcca tcaggatgta gacggttgaa
    28021 tctgccggat cgatctctat cgcctggaat gttggtaggg agtaactggc ccttggcagc
    28081 cggtcggtca ggtcgaccca ggtcgcaccg ccatcgacgc tcctgaagaa ctctcctttg
    28141 tccgaacctg catagatcgt ctgcgaatcc gtcgggtccg cggccagggc ggtaatccgg
    28201 taatccaaaa tgcccctgtt ggccgaagtc caggaatctc caccgtcgac ggtcttatag
    28261 acgccgtgcc cctgaccaac ccatccgccg gtgccggcgt agatcaccct cgagttcagg
    28321 ggatcgatgg ccagagatat gacatacgct cctatcctct catcgtcgct cttcggaagg
    28381 ccagcatcgc aagcttgcca actcatcccg ccgtttgtgc ttctgatcag gcctcctgca
    28441 gagaagacac cataaaggac catatcgtta tcaagagccg aatacaacct gcttctcagg
    28501 ctgccaaata cgccacagtt gccgactttt ctccagctgt tcgccccatc ttcgctggcg
    28561 aagatgtcac cacgaacccc aacgtcggca tgaagtatat cggagttgcc atggtgaaca
    28621 gctaaggcag agacgtcttc ggttggcagc cccgatgatg ccaggttcca ggtgagacct
    28681 gcgtcctcgc tcttatagac gcccgagccc gatccagaaa ggcctgcgcc cgcatatatc
    28741 accttcggat cgttgggatc tctcgcgaag tcgttaacct gacggggaag gtcgacgatc
    28801 ctctgccagc cctcgggaag ttgagacccg ccaagagacg agtcatttat attgttgttt
    28861 gaatattcat taggtgagcc cgtttggagc agaactaaca gaatcgcaga tatcgttatc
    28921 aaaccaaacc tcaattcaat cacctcgaat aatctaagat tcaatcttat taaaatagtt
    28981 tttgtaccct ccagtgggct gaaaagggca cccgactatt tgagctcaag tatgggatcg
    29041 ggcttaggga tgagagcgaa aggatcaagt cgcctcaagg agattcccca ctgcgaaacg
    29101 gagaggagga gagggagacg ccgggaaaca acagcgagat tgagaagagg ttgtgggacg
    29161 gggccgacga gctttgggtc cacggcttgc cgtcaatacg aattgccaac ttcaccaggg
    29221 tgcagcattt catctcggcc ccagcgcctg aacggatact ttccctccga aaacatcaaa
    29281 aaaagggtgc ttgaccatgg ccctcaagaa atccgaactc tactcctcac tgtggcagtc
    29341 ctgcgacgaa ctgc
//
LOCUS       DASM01000051           12197 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_67326, whole genome shotgun sequence.
ACCESSION   DASM01000051 DASM01000000
VERSION     DASM01000051.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12197)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12197)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12197
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_67326"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2464 a         3746 c         3875 g         2112 t
ORIGIN      
        1 ctgagttatg gcggctcgaa ccctgccttt tcgggcaatc catttaagcc ttttgatcag
       61 aaggggcctc atgcgcgttc ttaagaagag cctccggggc gatgaggggg agatctccct
      121 ggtggccgag tcgatcgacg acctctggca cctcaagtat ctgatcgccc ccggcgacct
      181 cgtcttcgcc ctcacccacc ggaaggcgac cccctccgcc gacaagctcc ggccggagaa
      241 gctggagagg aggcccgtcc ggctcgggat cagggtggag tcggtggagt ttcacacctc
      301 ctccagctgg ctccgggtcc acggggtgat cgaggtgggg caggacgtct cctcctacca
      361 caccctcaac ctcgagccgg ggtcggacct ctccatcgtc aagaggtggc gccccgacga
      421 gctggaccgg atccgggagg cggtcgccga ggcgaagcgg ccgaaggtgg tgatcgctct
      481 ggtggaggag ggggagacgg cgatcggggt cctccggcag ttcggggtcg agaccgtctc
      541 ggagatcagg atggggggcg gcaagggtat gagcggccgg ggcggcgacc ggaggaccga
      601 gttcctagac gaggcggcga aggagatcgc cagggtcgcc gcctccgact cgaaggttat
      661 cctggcgggg ccggggttca cgaaggagga cctgaagagg agggtcgact ccgcctatcc
      721 aggcctcgcc ccccggatca tcctctgcga cgcctccgcc atgggggtat cgggcttcca
      781 ggaggtcctc cgacggggcg ccatcgacca gatcctggag gagtcgaggc tcgccctgga
      841 gacgaccctc ctagaggccc tcctccggga gatcgccacc gacgggaggg cagcctacgg
      901 ccccgccgag gtgcgggggg cggcggagca gggggcggtc gagaccctga tggtctccga
      961 cgagcaggtc cgaagccccg agatggaccg gctgatgagg tccgtctccg acgcccgggg
     1021 tcgggtcgtg atcttcagct cggagttcga gccgggaaag agactctcgg ccctcggcgg
     1081 cgtcgcggcg atcacgcggt acaagatcgg ccggtgacgg gacaggttca tatatctccc
     1141 gggagggggt cggagggatg atccgctgcg atcggtgttc ggggagggcg gcgatattcc
     1201 agaggtactc ggggctccac ctctgccccc gccacttcga ggaggacgtc caccggaagg
     1261 tgaaggaggt cctccggacc tatcggatct tcggcagacc ctgcaggata gcggtggccg
     1321 tctccggcgg caaagacagc tcctccctcc tctcgatcct ccacgccctc ttctcctccc
     1381 gggaggatgt ggagctcgtc gccgtctcca tcgacgaggg gatcgccggc tacaggccga
     1441 ggaccctcgc cgcggcggcc tcggtggcca aggggctcgg ggtcgagcac gtggtgagga
     1501 gcctgggggg ggagttcggc gtcaccaccg acctcctcgc ctcctccggc cagccccagg
     1561 ggccctgcag cttctgcggc gtcctccgga aggggctcct caacaggacg gcccgggagc
     1621 tctccgccga cgccgtcgcc accggccaca acctcgacga cgaggcccag acggtgatgc
     1681 tcaactacct gaagggggac gtggaccggc tctacaggct ccggccgagg agggccctcc
     1741 cggggatggt ccccaggata aagccgctcc ggagggtgcc ggagaaggag atgggggtct
     1801 acgccatcgc ccacgccatc ccattcgaga ctcttgcctg cccctaccta tccagggcga
     1861 tgaggcagga ggtgaaggac ctcctgaacg acctggaggc gagacacccc gggacgaagt
     1921 actccatcgt caggggcttt gagaagatcc tcgcccggga tccaccggga tcctacgagg
     1981 tcgtcgcttg caccaggtgt ggcgagccct ccagcgacgg ggtctgcgcc acctgccgcc
     2041 tcctcgaacg gctacggccg gagcaaagtc tttaaatctt cagggcggag gagggtgcgg
     2101 gcctcggtgg ctcagctggc tagagcgagt gacttgtaat cactaggtcg cgtgttcgaa
     2161 tcacgcccgg ggcttaataa aattctatat atttcggagg cagaaggtgc gtcttatacc
     2221 tattctgatc ttctcgacga tggccatggc gtcggccgcc gtcgaccccc tcaacctcag
     2281 cttcaccggg gacctggacg agctggaggc gctcctggcg ggggcggata tcggcctcgt
     2341 caaccgatcg gcggcggggc cgggccagag cggatccgac cccgggatcg agccgccctt
     2401 cctccatctt ctgagaaacg agagcatctt cctcgatttc gccaacctca ccgccccggc
     2461 gttccccgta accttcgggg cggatgaggc ggagaacgag tccttcttcg agatcctcct
     2521 ccgggactgg tggtgaggct cctcgaagga aaactataat cctcttcaga tacgaacggg
     2581 atgatctatg agggacttcg ccaaggagat cttagaggtg ctggaagaga acgccagggc
     2641 cacccctgga gagatcgcga ccctgctggg ccgcccggag ggggaggtgg cagccgagat
     2701 caagagccta gaggactcgg ggatcataag gaagtacatg acgatgatca actgggagaa
     2761 ggtcgacgac ggctacgtct acgccatagt cgagctgaag gtatccctat cgagggacaa
     2821 ggggtacgac gccatcgccg agaggatcgc ccggttctcc gaggtccagt cggtgaggct
     2881 gatatccggc gaccacgaca tatccttcac cgtccggggg aggtcgatga aggacgtcgc
     2941 cttcttcgtc accgagaaga tcgccaccct ggagggggtc gaggcgacct gcacccactt
     3001 cgtcctcaag agctacaagg agcacggggt catcctctac gacagggtcc agcccaagag
     3061 actggtggtc accgcatgac gaggaccgat aaaggcggaa aaaacaagaa gctggcgatg
     3121 gagaagcccg ccgtctggag ccccgagaac cacctctcca ctacggttaa ggagatgccc
     3181 ccctcgggga tcaggaagtt cttcgacatc gtggtgggga tggaggacgt tatcagcctc
     3241 ggcgtggggg agccggactt tcccaccccc tggcacatcc gggaggcgtg catctactcc
     3301 ctggagtgcg gccacacctc ctacacctcg aacaaggggc tcatcgagct gagggagatc
     3361 ctggcggagt cggtgaagga ggagatcgaa ctcgattacg accccgagga ggagattctc
     3421 atcaccaccg gggtctccga ggccgtcgac ctcgcctttc gggcgaccct gaaccccggg
     3481 gacgaggtga tcgtccccca gccctgctac gtcgcctacg tccccgacgt cctcctggcg
     3541 gggggggtcc caaaacccgt ggcgacgcgg ctctccgagg agttcaagct ccgccccgag
     3601 gacttccggg acgccatcgg cgagaggacg aaggccctcg tcctcagcta ccccaacaat
     3661 cccaccgggg cgatcatgac ccgggaggac ctggaggaga tcgcagacct ggtggtcgaa
     3721 aacgacctcc tggtcatctc cgacgaggtc tacgacaagc tcacctacga cggcaagcac
     3781 gcgagcttcg cccagctgga ggggatgaga gagaggacgg tcctcctaaa cggcctatcc
     3841 aagtcctgcg ccatgaccgg ctggaggata ggctacgccc tcggcgactc cgccatcatc
     3901 ggggctatga ccaaggtcca ccagtacacc atgctctgcg cccccaccat ggcccaggtg
     3961 gcggcggtgg aggcgctccg ccggggcgaa tccgaggtga aggcgatgaa gcgggagtac
     4021 gaccggcggc ggaggctctt cgtctcggag ctggagaggc tcggcctccc ctgcttcgag
     4081 ccgaaggggg ccttctacgc cttcccctcc atcgagggga cggggctcac ctccgaggag
     4141 ttcgccgagg agctcttgaa ggagcagagg gtggcagcgg tcccaggaaa cgccttcggt
     4201 gagagcggcg aggggttcct ccgctgctct tacgccacct ccagggagga gatcctggag
     4261 gcggtggaca ggatcgagcg gttcctggcg gacctgggcg cctgagccgg ggggctcaaa
     4321 agggtttaat acccccctcc cgtttcgacc tcgatgctca agtcccgtct ggtggatgga
     4381 gagaaggtgg ataggaggct ggcccgcctc gcccgcctcg gcctctcccc caacgcctgg
     4441 accggcctcg ccctggccgt cgccctcggc ggcttcgtag ccctctggag aggccagctc
     4501 ctccccggcc tccttctctt cctggtatcg gggggcctgg acgtcgtcga tggggcggtg
     4561 gcgagggcaa ccggccggac ctccgtggcc ggtgccttcc tcgacggagt cctcgacagg
     4621 tacgtcgagg ctctcctgat cctggggctt ctattctata tcggccccga ggccgagttc
     4681 atcatcccca tgagcggctg gatcgcggcc ctcatcttcg gtgccgtcat gacctccttc
     4741 gttcgggcct acgccgacca ccggggcctc gtcaaggacc aggccatcct ggtgaaggag
     4801 atgggagggc tcctggagag ggcggagagg ctgatcctca tctacgccgg gatgatcgga
     4861 gccctcctat tcggcgacct ctggcttttt ggggcgattg ccatcgccgc cgtcctctcc
     4921 aacctcaccg cagtccagcg gatatgcatc gcgatgaagt acggaggggg ggtccagccc
     4981 cgggcagata aaaagagaaa aaggacggtc tgaactatcg acggccccgc cccgacccaa
     5041 agcgggagag ggggcccaga cgtcgctcaa cgtccgggcc ccattctgtc caatgggcac
     5101 agtctgggca cggcgtttca gcggtggagg tcaggatctc gccttccgcg cggcaatggg
     5161 ggatgatatc ttatttagta ttaagctttt cggttaagtt gctacatacc cgggtctctg
     5221 ggaccggggc tatgaactta ccccatccct cacggaccga gatcttcgcc aaccggctat
     5281 ggaaagagct ggccgacctc aggaacggtc cgggccttca gagggcggtg cctcgaccct
     5341 ctcgagatag agccgcccgg tggtggtggg cctgtagacg ccatctacgg cctcgacgac
     5401 cagggccctc tccagcatag cgaggtggaa ggcggcaagg cccccatcga tccccaacat
     5461 ctcggcgatc tcctccgggg agccgacgcc atcgtcgacc atcttcaata tggccctcct
     5521 cgccctcagc tggaggacct ccagggcgaa cctgtgccct tcggccgaat cccaccagcg
     5581 cacctccccc ccgcccccct cgggctggtc gccatcgggt gggccaaagt cgatatccac
     5641 gggggagcta tcttctgggg ccatctgaga tcgtctcttt cggcaaggaa aaaaaggtgg
     5701 cggcagaagg aggcaatttt gaaatatgtc cagggatcag aagaggaagc tgatgaagcc
     5761 gaggcggcca gagacggaga aaacttcgat ctcgatccga aagtatctca agccagggga
     5821 catcgggatg gtgatccacc tccacggggt cgtctactcc gaggagcagg ggtgggacga
     5881 gaccttcgac ggctacgtcg cggggccgat cgccaggttt gctacatccc ggtcggagcg
     5941 ggagaggatc tggatcgtcg aggtggagaa ggctggggag ggacgggtcg tcgggtccgc
     6001 tgccgtcgtc gaagcctctg aggaggaggc ccagctccgg tggctcctcc tcgccccgga
     6061 ggtgaggggg ctcggcctcg gccggaggct ggcggaggag gcggtggagt tctcccgatc
     6121 tgtcgggtac ccctccatat tcctctggac cgtccggggc ctggaggcgg cggagagcct
     6181 ctacgaatcc ctcggctttg aagagagcga ggagaagacc tccgagatct ggggcggggt
     6241 cgtcaccgag gtgaggtacg acctcaagct ctgagatgtt tttatccccg gaggcgagag
     6301 gaatggaaga agggatctat tggagatgct gactgatgac cgaagagata ttcgaggcga
     6361 tccgaaggag ggtcgcagaa gagccctacg ctgaaaagat ggggctcaag ctggtggagg
     6421 tggccgaagg gtacgccctt attgagatga ggttcaccga agagatggag aacatcttcg
     6481 ggatggccca cggcggagct atcttctccc tcatcgacga ggcctttgag gtggcctcaa
     6541 actcccacgg gaccgtctcc gtcgccctca gcatgagcgt cacttacgtc agctccccca
     6601 agaaggggga gaccctccag gccgaggctg aagaggtgag cagatcgaac cgggttgcga
     6661 cctacgatat ccgggtcaag accgctggcg gcgacctcat cgccatctgc caggccctag
     6721 tttacagaaa acgggaccct ctgccgttta tctgatggac gcctatgatc cgagcaggca
     6781 ggggggagat ggatcaggat gcgaagagat aaagaaagcg gcgtataaaa aggcaggccg
     6841 tatccagggt ggtctcgggt cttggagatc ggagcgtgga atccggagat cacattctcc
     6901 gaccccgagg gaatttgaag gccttcagcc gcgatagcat ttttttctct ccaaaggcgg
     6961 cgggaactaa ggccacatcc gatctccgac cgaaagtttc agcgtactct ctggtgacga
     7021 aacccagatc gttgaggcct cggccgtcga agctgatcgc gaggtatacg ttcgaggaga
     7081 cgaacttgtc gaactcctta tcgttcttgg ggaccgtggc aagagggaga tcctgccacg
     7141 ccagccagga gtaacacttc tgaagggcga gttcgccctc cagaccttcc acgacgggcc
     7201 atccctgggg ggtccattcg atcttatcag cgatttcggc tacgatcctt ttcccatcga
     7261 tcgtcaaatc tccattgctc tctcccctca tctcggatca cttcttcgat cattctctcg
     7321 ctcacgctga agttctcgcc cttgttctat ctgcaattcc ttcaacctgg accggagagc
     7381 actcatgctc accttgtgga ccaccgatat ggccataaga tccccgtaac cttccgcagc
     7441 atccttgagc ggctcgtcca gattctccac taccgttgat ctttggtttg atgcctcgct
     7501 catattcatc tcagctcaat tttaaattat atttacgcca tattcgacat tctcaaggcc
     7561 taaggactca ccccagaaat cgaccgagat ctctcaacag aagatcccca tccctagaaa
     7621 tcgaagagat cgaaggcgag agagacagac tgtctcatat taaagcagtc gtgggcagca
     7681 ggatcgctcc tctgaccgcc aacgactatg aagatcgatg attgcttcag accttgtggg
     7741 cgatggcgaa cctttgcatg accttatcga ccacgatttt ggtcttgtcg ccaacgccgg
     7801 tctccgggac gaagatgacg aaaccctcga tcctggctat gccgtcgccc tgctttgata
     7861 tgtcctcgat ctcaacatca tattcctttc cagtctccac cggcgcagag ggaccaaaac
     7921 cgctggcgcc aaaagagcct ctagtattac cgtacaaact taacaccatc ctttgcgaaa
     7981 aaccataaaa cagaatagct taacgcaagg caccagaaag gtctctgact atttaagtct
     8041 tcgcacctgg ccagagatcg gaggagttcg aagaaggatc gcccatcacc ggcccccatc
     8101 gccgagacgt caagtgatcc gcccgaacgg gaggatgaaa tctacgaacc cctcccacct
     8161 caaagggtcc tctgcatcca tacgacgtca aagtcttcac cccacttcct cccgatcgcc
     8221 ttgaaccgac cgcattctcg gaaccccagc cgtcggtgga aggcgatgct cccatcgttt
     8281 cgggaggaga tgccggcgag gatagaggtg atgcctctcc ccttcgcctc atcgatgagc
     8341 cggagagcca aagatctgcc tatccccatc ccccccggga ttcaggatgg acgaagatgg
     8401 tgatctcggc ggtccgggag aaggcgggca tgggatggtg ggagcggagg agcgcaaaac
     8461 cgaggacccg tcccctggcg cccctggcga caaaggcggg atagccccta gaggcctcca
     8521 tcaggaggtc gaagaacttc ccaggcaccc gatcttccgg gaaggcagcg aagctgttat
     8581 cgacgtagaa gttgaagatc tcgacgatct cttcacgatc ctccgaggat atcggttcaa
     8641 gctccaagtc catggattac ccccccgggc tcccctcccg gggagaggat tgcttattag
     8701 cttttctgct gaagggcccc gcccgacgga aaccgtgatt acccctcccg accatctccc
     8761 ctccggagtc catatctcat gatcacaaaa gtctacatca cccactctga ggaggacgag
     8821 cccctggccc gcgaactggc cgaggccctc tggcgggtca gcctggagag cttcgtcgcc
     8881 atataccggc gggcccgcgg aatctcgccg ggggagaggg tgaggtttgc catccgccac
     8941 tgtgactgcg tcatcgcgct gcttactttg gacggcgtcg tatccccaag ggtaaaccag
     9001 gagatcggct tcgccgccgg gatcgaccaa ttggtgatcc ccctcctgga gaaggaggcg
     9061 gagctccccg tcatgataag gcacctcaag ccgattacat tcagccggga gggctactcc
     9121 gacgccctcg gcgaggtgat actaaatatg agggacctca cggacctcga gtggctgaag
     9181 ataaagtgcc ccctctgcgg cgaggagatg acccagtacc tcccgcctca ggagctggtg
     9241 gagagggcgg tcctggccgg gacgggcctc gacacgatct gcacctactg cgagagcgcc
     9301 ctcaccctcg acccccggac cttcaagccc atccattgac ccggggcccg ggacccgcgc
     9361 caccaccacc tcaacgcctc gacgagagac ataatatagg ggaggaggcg tggaggtggg
     9421 gacgaggcga gcaattcgat gtcatcagat gagggtggga cgggggatcc ggccccaaaa
     9481 ctggccactg gacggccttt caaatcgacc cgcataccgg ccgccggagt gaccccattt
     9541 tcctctccaa acctttttat atcaggagta ggtcttgggg gtaagatccg atgatcggtg
     9601 gctgcgagac gtccatcttg atcccgtcct cagcgacgat ggagtcgcgg gacgagcggg
     9661 tacgcaccct caaggtgggc atgatcgcaa gagcagctgc ggtatttcgg gtggatcgga
     9721 tagtcatata tcgcgacgag gagttcgacg actcccggtt catatcccgg gtcctgcagt
     9781 acgcagagac gccccagtac ctccgaaaga ggctctttcc tcgggagagg gccctgaggt
     9841 acgtcggcat cctgccgccg ctgcgcaccc ctcatcatcc cacaagctca aaggtttcaa
     9901 cgctcaaggt cggcgagtat cgagtaggtt tagtagtcga cgaagtggga tctgacgacg
     9961 gcgcatgggt ggagataggt gttgagcgcc ctttgcccct cagaaccgcg aagcggtttt
    10021 ctgctgggca gcgtctgaac gtcagagtct tttcgctgaa acccctcgcc gcggagcctg
    10081 tagaccgatc ggagataccc cattactggg gctacgagac cgaggtcatc ccagacctgg
    10141 acgggcacgt ccgctcgagc gagcggctgg tggtggccac gtccagattg gggacgccag
    10201 caaccccaga cctcctcgcg cgggtggtcc gccagggcca agggcgaggt ctggaactgg
    10261 tctacggatc gcccgctcgc ggcgttgatg cgttcttgag cagcgaaacg atggaaggat
    10321 gttgcatgat taacaccata ccgcatcagg gaaccgagac ggtgaggctc gaagaggcga
    10381 tattcgccac cctctcccag gtcaacctga tgcgatcact ggagggctaa atggcaacca
    10441 tacaccgacc aaggcgaggt tcgctggcct tcagcccccg aaagcgggct aagagccagg
    10501 tccccaggac gagatactgg gccgctggcg aggagaaggc cagaatggac ggttttgccg
    10561 gttacaaggc cggcatgacc catatcatca tgatcgacga caagcccaac agcctcaccg
    10621 aggggatgga gatcagcgtc ccggtgacga tcctggagac gccacccctg agcatagccg
    10681 cccttcgcgt ctacgagaag tacaacggcg gcgtgagggc tgcgggagag gcctggtccg
    10741 ataagctgga cccatccctg gcacggtcga tcaccgtccc caagaacaag aggggagccg
    10801 ccgtcgacga gatcggggcg ataattggag atatggaaga gctgcgattg atcgctcaca
    10861 ccaaccccaa gcttctgacg ggggtaccca agaagaatcc agacctgatg gagatccagg
    10921 tgaacggcgg caacatcgcc aaccagttcg agctggcaaa gagcctcctg ggatcctcgg
    10981 ttccgatctc cagcatcttc ggccccggat cgatcatcga cgtctccgcc atcaccaaag
    11041 ggaagggagt ccagggcccc gtcaagcggt ggggtatcaa ccttcagaag aggaagcact
    11101 cccggggcgg caagcggagg cacataggca acctgggccc ctggaacccc caccacgtca
    11161 ggtggaccgt ccccctcctg ggccagatgg gctaccacca gaggaccgag ttcaataaaa
    11221 gggtcctggc gatcggatcc gacggcagcg ccatcacccc cgagggcgga tttcctggat
    11281 acgggatcat ccggggggag tacgccatcc tcagcggcag cgtcccgggc cccagcaaga
    11341 ggctggtcag gatgaggtcc gcggtcagag ccaaggacgc cgacctgaag acgccccagg
    11401 tcctctacgt cagcagagac tccaagcagg gattgtgatg aaggctaaag taatcgatct
    11461 atccggaaat gaggtcggag agatggacct gccatcggtc ttcgacgagg agttccggcc
    11521 cgatctcata aagagggcag tgctggcggc ccaggccaac cggctccagc cctacggtcc
    11581 gagcgatctc gcaggcctca aaacctctgc cctctcctgg ggaacggggc gcggcgcctc
    11641 ccgagtcccg aggatcctga acggaaggcg agctgcgagg gtcccccagg ccaagggcgg
    11701 caggagagcc caccccccga agccccagac ggacaggacc gagaaggtga acgccaagga
    11761 gcggaggaag gcgatccgat cggcgatagc cgccaccgcg aacgccgacc tcgtccgggg
    11821 gaggggccac ctcttctcag gcgagccgat cgtcgtcgcc aaagacgacc tggaaggagt
    11881 cgtgaagacg gcggaggtca gaaagttcct ggcggcctgc ggccggtggg acgatgtcat
    11941 gagggcgaaa gatgggaaga ccgtcagggc cggcagaggc aagatgcgag gaagaaggta
    12001 caagcagccg aagagcctgc tgatagtggc gggccgagag ggcgggctga tcagggcctc
    12061 gcgaaacctt ccgggcgtcg acgtcaccac cgtcgatagg ctgaacgtcg agcttttggc
    12121 ccccggaacc cacgccggaa ggctcaccgt ctggaccgaa tcctctctga ggtggctggg
    12181 tgagacctat ggtcatt
//
LOCUS       DASM01000052            8381 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_127860, whole genome shotgun sequence.
ACCESSION   DASM01000052 DASM01000000
VERSION     DASM01000052.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_127860"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1533 a         2698 c         2722 g         1428 t
ORIGIN      
        1 gtcttcgaga agaccgtcga gggcggcacc tacagctacc ttctgatgga ctgcgaggcg
       61 ggggtggagc acatcgcaag gaagacctcg gggaagatca acgacgccat catcgtcacc
      121 gacgcgtcca aggtgagcct cgacaccctg aagcggatta gggatgtcgc ctcggaggtg
      181 ggggcgacga tcgagcggta ctacgtcgtc gccaacaaga ccaggcccga gatgctggag
      241 aaggtcaaag aggcagcggg gagcctcggc ttcgagtacc tgggcgccat ccccgaggac
      301 cccaacttca cggaggtcgc ctaccagggg aagaccgtct tcgacctccc cgacgactcc
      361 ccggcggtcg aggctgcgcg aaagatcact gaccagatcc tgggagcctg agccctacca
      421 agacctaaga ccatgagagg aagagcgatc tcaattctca tctgccttgc cgcctgcctc
      481 gtctcgggct gcatggatga ggcgggggag ggagccagcc cggaggggtc ggcctccggc
      541 ctcaccgtgg cgataacctc ccccgccacc ggctcgctcc tctccgggga tgagcctatc
      601 aggttcgagg gggaggcgac gggcggaagg aagccctacg cttaccggtg gtcgtcctcc
      661 ttggatggga ccctctccac caggagttcc ttcgaagccc cgccgtcgga gatgagccgg
      721 ggccggtacg tcgtcaccct gacggcgacg gacggggccg gggcgagggg cgaggcgagc
      781 atcaccgtga cggtcctcta gggaggacct cgatatatcc ggcccctggt cggcggctcc
      841 cttccccagc cgatctccgc cggtgccata aaagaccaat tcttatttgc cagggagggc
      901 tggctcgatg ccttataccc aggacgacag caccgatagc tggaaccgca atgcagatgc
      961 ctggcaccgg aacttcggca ggaacgatcc caaccgtcga gatctcctgg accccataat
     1021 cctcgaggcc ctgggagaag tcgggggtaa acggatcctc gatgccggct gtggggatgg
     1081 ctacctcagc cggaagctgg cagaactggg cgccgccgtc accgccgtgg aggcatcgtc
     1141 tcagatgctg gcatttgctc tggcagaaga ggaattagag cccctatcca tcaggtatca
     1201 tgaaggggat atagcccgcc tccccttttt ggccgaccgc tcttttgacg ccgtggtcac
     1261 caacaacgtc atccaggacg ccgcagattt cgagggggcc ttcagggagt tcagccgcct
     1321 cctccgacca ggaggcatct atctccacat cgtaaaccat ccgtgtttct cggcccccgt
     1381 cttcggctgg gtgaaggacg gcgaaggaag aaagctgtac aggaaggtcg accgctactt
     1441 cgaccgggga ccgttcctcg tcccctgggg cccgagctcc tgcatggagc cgacggtggc
     1501 ctggcatcgg accctcgggg atacggtgaa ctccctgatc tcttctggat ttcggctctt
     1561 gaggctcctg gaacccgaac ctcccgaatc ctggagaacc ctccatccgg agcggcttga
     1621 cgccgcccgg gtccccgatt ttctcgtcct cgtctggaga aaggaggagg ccgatcgcta
     1681 aaggagggtc atagcctcgc ctcggcgagg gcccggccgc aggagcagcc cctctcctcg
     1741 gggatcatcg agatggcggc ctctatgacc tcccagagaa gccgggcgga tcttgccatc
     1801 tcggttgtga tctccgccgc agacatcgaa tctttcctca tcccggcggc ggggtttgtt
     1861 acgatgcaga tcgaggcgta acagagctcc agctcccggg ctaggacgac ctcggggtag
     1921 ccggtcatgc cgacgacgtc gccaaacctc cgaagcatcc tgatctgagc cgggctctcc
     1981 aggtggggcc cctcggtgca gacgtatatc ccgtcggaga cgagaacgcc ggccgcctcc
     2041 gcccccgccg ccagggcccg cctcacttcg gggcagtagg gggccgtcag atcgacgtgg
     2101 accgccccct cctcaaagaa ggtcgagggc ctcgatttgg tgaaatcgac gaagtcggtg
     2161 gggatgacat agcttccgac ggggtgccag gccatcgacc ccaccgtgtt ggtggagacg
     2221 acccggaccg cccccgaggc cctcgccgcc cagaggaggg cccggtagtt cacccggtgg
     2281 ggtgggaggt gggcctcgcc gtggcgcggg atgaagagga cctcccggcc gcaaagttcc
     2341 gtcgccgccg ccatcaccgg gccgtagggg gtatcgatga ggagaggctc gcccccgacc
     2401 tcaaaccccg ttcctccgat caccgcgatc ttaccattca tgttcggtag gaagatcctc
     2461 tgaagttata ggacttctcc ccggggggag atatcggcgg catcctcgat ccaatcgggg
     2521 gctcttttaa ggtcgacggc gcctatcggg cgcaggtatg gcgaaaccta cataagcccc
     2581 gtccacatct tgcctctgaa acggccgtcg gacgacggct acagtatccc ccccaaatag
     2641 cgggcccttt tgaggagatg ccagatggag tttaccgccc aagagaagta cgaattcaaa
     2701 cggctgctcg aagacctgag gagcaagagc ggtaggggca cagagctgat ctcgctctac
     2761 atcccccccg acaagcagat atcggacgtg acggcccagc tccgggacga gcacggccag
     2821 gcgtccaaca tcaagtccaa gctcacccgg acgaacgtcc agagcgccct cgagtcggtg
     2881 atggcgaggc tccggctgat cccaaaggcc cctcccaatg gggtcgccat cttcgtcggg
     2941 gccgtcgaca tcggcggaaa caagaccgac atgtacagcc gggtcctgga gcctccggag
     3001 ccgatcgtca cctaccggta ccactgcgac tcatcgttcc ttctaacccc cctggaggat
     3061 atgctcgccg acaagaagac cttcggcctc atcgtcctcg accggaggga ggcgacgatc
     3121 gggaccctcc ggggaaagca ggtggaggcc ataaagcacc tcacctcctc ggtccccgga
     3181 aagcagagga agggcgggca gagcagccac aggttccagc agctgaggct gatcgcgatc
     3241 cacgaattct acaagaggat cggagacagc gccaccgagg cgttcctctc catcgacaga
     3301 aaagacctgg aggggatcct gatcggaggg ccgtccccga cgaaggagga gttcgtcgac
     3361 ggcgccttcc tccaccacga gctccagcag aggatcctgg aggccctgga cgtcacctac
     3421 accgacgagt cggggctcta cgagctggtg gaggcggccc aggagcggct cctcgacctg
     3481 gagctgaccc aggagaagct ggtcatgagg aggttcatga aggagctggt gagcgataag
     3541 ggcctcgcct cctacgggga gaaggaggtc cgggagaacc tgacgatggg ggcggtagac
     3601 accctcctcc tctccgagga cctccggaag acgagggccc ggatcgtctg caccaacgcc
     3661 agctgcgact tctccgaaga tgccacgcga tcgccgaaca gcccgccgcc ggggacctgt
     3721 ctgaggtgcg ggtctcagct cgccctagcc gatgaggtgg acatcatatc cgacctctcc
     3781 cggatggcgg aggagagcgg aagctccgtc aagatcatat ccaccgactt cgaggagggg
     3841 gcccagctcc tccgggcctt cggcgggatc gcggcgatcc tgcggttcag gacgagcagc
     3901 cgctgacccc cgcctcccct aagcctaccc aactctaaaa taccaggacg aaagacccca
     3961 taaagatgat cgaggtgtcg ctgagggcgg cggtcaggcc caccgagagc cccgagaagg
     4021 tggccggggc cctggaggcc ctatttccgg ggatcgaggt cgatgtccaa gggggcttta
     4081 tcagcgggcg ggggggcgcg gccagccttg ccgccttcca ccgcctcctg agggagcaga
     4141 ggatcctgga taccgcgagg tcggtgatgc tccagggagt ggtcggagac gccgtccagt
     4201 tcaggctgaa taagcaggcg gccaccgtcg ggaggataaa ttttccgcca gaggaggagc
     4261 ctctcggatc gatccacgtc gagatcaggg gggcggcctc cctcatagac tggcttgccc
     4321 cccggaccgt cgacgggaag cccatcgagg agatcgagct ggaggatgga gatcatgttt
     4381 agcttctcct ccagcgccct cgtcgatcag cccttcgact gggcctatca gctggaggat
     4441 ctcggctact ccggctggga gatcgtcaac gagggaaggc agaggctgac ggccgagacc
     4501 atacccctcg cccgggagat cgtcgagacg accgacctcg tcatcaccat ccacctcccc
     4561 tactccgacc tcaacctcgc cagcatgaac cagccgatct gggaggagac ggtccggcag
     4621 atgaaggagt gcatcagcct cgcctccgag ttctgcggcc tcgccgtcgt ccatcccggc
     4681 tacctctcac ccctgggaaa gcagatgccg gaccgggcct gggagcagaa cattctgggc
     4741 ttgcaggaga tctgcgacca cgccgaggag ttcgggatga ggatcgccgt cgagaatatg
     4801 ctgaacatcg agtcgatgct gggaaggacc cccgaggaga tcctggggat cctcgagaac
     4861 gtccggcggg agaacctggg gttcgtcttc gacgtcggcc acgccaacac caacgggaac
     4921 gtcgactcgt ttctggccct gaaggagaag atggtccacg tccacgtcca cgacaacaag
     4981 ggggcgaggg acgagcacct gccggtcaag agcgggaacg tcaactggcg gagggtggcg
     5041 aagggccttg aggggtacga gggcaggttc gtcaccgagg cccggaccct ggtccagggg
     5101 gcccagtcca tgaggaactt gaaggccttt ttggagggct ccgcatgacg ccgtcggccg
     5161 acgagctggc cgaggagata tcccgccatc tagaggagaa ggacagggcc cgggaggagg
     5221 cgctggccct gacgagggcg gtggtcagat cgtcgggcgc agccatcagg tcagtccacc
     5281 ggggggagct cgaccgggcc ctcgacctcg ccggccgggc gggggaggac ctagcctcta
     5341 tcctgaacct cctgaaagag catcccgacg tccgctactc cggcctcgtc gacggcgcgg
     5401 agcaggagta cgccgaggcg aggatcgtct actccctcat caccgaggat aggatccccc
     5461 atccccggga ggtgggggtc gagatgacga gctacttggc ggggctcggc gacgccgtcg
     5521 gggagctgag gaggcacatc ctcgacctga tccggttggg gaggccggag gagggggagg
     5581 tccacctcgc ggcgatggag gagatctacc atctgttgat gtccttcgac taccccgacg
     5641 ccgtcaccaa ggggatgcgg cggaaggggg acgtcgcccg ctccctcctg gatcggaccc
     5701 ggggcgacct caccaacgcc atcggccagc ggaggctgga ggcggagctc cgggaggtgg
     5761 aggagaggat gaggagagtt aattcagcca aataatttta ttttctaaga gataagtcat
     5821 ttccgcttcg ccagcgtcag atccccgtgg ggcatgacga tcgtcctcgc atcccgcccc
     5881 tgcctctccc gggccatggc cagagcctct tcgaggctgt tcgcgtgccg gaagaagacc
     5941 atctccgcaa gctcgggcct cagggaggag accaggatga ggtctttttc ctgggactct
     6001 cgcgcgatca gggccgcctt gtgccccccg aactcgtact cccggccgaa cctctcgacg
     6061 cagtcctcgg cgcactttgc ctcccgcgcc caccgctcga agacggggtg gccgaggcct
     6121 tcagcgcact cggcgacgag gatcagagag ccgccgggga ggaccgcctc cctcgcgttc
     6181 tccatcgcct tctgggcctg gaagaggttt atgtccttgg ggcggccgcc ggcgcaggcg
     6241 acgacgatct cggcggggcg gacggccctc tggtacatct cgtcgacgac tttggccccc
     6301 gcgcggtggg cttttacgaa gtcgccgccg acggcccgga cgatctcctt cttgctgttg
     6361 aggacgacgt tgaggatcag gtcgaggcct ccaatttttg ccgcctcctc catgtcgagg
     6421 cggacggggt tgtcgaggcg gccggacttc gcccgggggt cggccatcat ggcgtggttc
     6481 ttgttgatcg acctctcgga gctgacgccg gggaggaggg ctttcgcccc gccgctgtag
     6541 ccggcgtagt agtggtactc gatgtttccc gtcccgatct tgatgtcgga tgccgccacc
     6601 tcctccagga tctcgaccgg ggtactccgg ctcgtctcgc caaggtggat gcatcggtcc
     6661 ttgtcgtgct ggatggaggg gaggctggcg gagctgccca ggagcctgtc catctcctgg
     6721 tcggtcatcc tccggtgggt ccccaggccg aagacgacga agatctctga tatgccgagg
     6781 gcctcgagcc tccgcaccag gggcggcagg atcctggagg tcggcgcggg ccgggttatg
     6841 tcgctcgcca ggatggcggc cctcctggag ccggccagat cctccaggcc gggtccgatc
     6901 ggcacgttga gggccctctc gatctctccc gtctcgtcgg gggctccggg ccgctcctct
     6961 gggaggacga cctcaaagcc ctcggcaccg tccaccggga ggcggatccg gccggcgccg
     7021 tacccgaggt ccattcaggc cctccagacc ttggcccggg tatggacgtc ggagaaggcc
     7081 tcgaatatgg gcctcgcggt ggtgtaggtg aagaagtcgg ccccgacggc gtgggaggcc
     7141 tcccggatcc gggcttcgtg ggccgccatc ctcgccgcgt actcatctct caccttgggc
     7201 gtcaccctgg tcatcaccgc ctccttcgac tccatatcga cgaaccggag gtccccctcg
     7261 aagtcgaggg ccgcctccgc cgggtcgaga acctggatca ggatcaggtc gtgggccgag
     7321 agcttgtaga tcccggcgag gaggctctcg atctcttcga ggaggtcgga gatcaccacc
     7381 accaggctcg tcgacctgat ggtgaggtcg aactgctccg ccatcctccg aaagtccgtc
     7441 cccccagaag gggcggtcct gtccaggtcc tccatggcag aaaagagatt cctccgcccg
     7501 cctttcgtct cgccaggctc cagcatctcc ccgaacttgg agatggagaa cttctcgttc
     7561 tcttttatag ccaggtaagc gaaccccacc gccagcttcg aggcgaaggc gaacttctcg
     7621 ccgaaggcca tgctgctgct cgcgtccagg agtatgtgga cggcgaggct cctctcctcc
     7681 tcatgctccc ggacgtagag cttctcagtc ctggcgtaga ccttccagtc gacgaacttg
     7741 aagtcgtccc ccttccgata ctcccggtag ccgacggggc tgatcccccg gccgaccttc
     7801 accgacctcc gggccccggt gtaggcggtc gacaccctct ttcggacgag gagggtgaac
     7861 cggtccagct cagcgagaaa ctccgtatcc atcggcttgc ctcagatcgc cttcatgatc
     7921 tccgagatca cctggtcggt cgtgaccccc ctcctctcgg agtcgaaggt gaggatgatc
     7981 ctgtgccgga agatggggcg ggccatcacc tccaggtcgg cccgatcgac gtagtccctc
     8041 ccggagagga gggcccgggc cttggaggcg aggatgaggc cgatggaggc ccgagggctt
     8101 gcgccgtatt cgaccccctc ccagctccgg gaggagacga cggcctttat cgccgccctc
     8161 ttcaggtcgt cggagatggg gacgtccctt acaagctgct ggagggataa aagctccctg
     8221 cgggtgatca ccctctccac cttcggctcg gcgtggccgg tgtacctctc catgatctgg
     8281 tactcctcct ccgggctcgg gtagtcgatg aatactttca ggaggaaacg gtcgagttgg
     8341 gcctccggca gcgggaacgt cccctccatc tcgatggggt t
//
LOCUS       DASM01000053            9000 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_94468, whole genome shotgun sequence.
ACCESSION   DASM01000053 DASM01000000
VERSION     DASM01000053.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_94468"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    8711..8727
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1680 a         2799 c         2933 g         1571 t
ORIGIN      
        1 ggtcctcacg atcttgaatc gatggacgct tccaagaggg aggggctcgt tgaccacaat
       61 cttcctgtag ttggctgacc tcgccatcac cgtcccccct cttcccatct ccgtcacctg
      121 cgcgatgatc tcctccccga ggtagcggcc gtttcttcgc cctgctatct ccttccagag
      181 gcgggtcatc ctcctcgagc ggtccttctt gagccgggac ggcatatcct tcagcctgct
      241 cgcctccgtc ccggggcggg gggagtacat ggtgacgttg accttgtcag gctctgcctt
      301 tttgatcagc tcctcggtgg cggcaaagtc ctcctccccc tccccggggt ggccggcgat
      361 gacgtcggtg tagagggtga ggccggggaa ggccgccctg agccgcgccg ccataaagag
      421 gaaatcctgg ggggtatagc ccctcctcat cgatcggagt acctccgccg atccggactg
      481 gaccggaagg tggaggaact tgtagacctt ggggtcgtcg taggagcgga tcagctcctc
      541 caagatcggc ttcacctggt ccgggttcat cattcctacc ctgatccgaa agtcgccttc
      601 gatttcggcg acggcgtcga tcagctccgg gagggaggaa cccaggtcca ggccgtaggc
      661 agcggcgtcc tgggaggcga gctggacctc caccgccccc tctttcagcc ggcgacggac
      721 cacctcggcc acctcctgga cggtcctgct ccggaggggg ccccgcgccc tccggacgat
      781 gcagtagctg caacttccga ggcacccctc cgatatgttg acgacggtgc agaggtccct
      841 cgccatccgt ccggatcccg caagctccgg ctctgggggg aaggcctcgc cgatcctccg
      901 ccccgagggg ggggagagga tccccagcac ctcgcggcac ctcaggtccc ggaccgcctc
      961 ggggatcgcc gatgggaggc agccggcgac gatgagcctc tccccctgga gctctgagag
     1021 cctcctccga atatccctct ccgtcctttc ggtgaccgcg cagctgttga cgatcaccag
     1081 gtccgcctcc tccagagacg atcgtcggtg gcccgcctcc accagggcgg aggcgaaggc
     1141 ctccgagttc ccccggtttg cggcacagcc gtaggtctcg atataaaatc tcatccggga
     1201 tctgtccatg gaacccgggg ccggggcggc atggggcggc ttctcatcca tctcatctcc
     1261 cggagccttg gccaccctct ggtggcaggg cggactccga tccgccagcc tcctcctcga
     1321 cctccggtgg atcggtcccc tccgtcggcg tcccgtctcc agaggatgcc gccatcttct
     1381 cctccttgtg gacctgatat accccgacga ctacccccat cagggcgggg gcgaggaaga
     1441 accctccaag ccccgccacc agaactcccc cgacgaaggc gaggaccacc aggagaggat
     1501 gtatcgtcga cttaatcgag actatgtagg gcctgatgat gaggtcggag gggatgtaga
     1561 tcatcgccga ggagacgacg aagaagagga tcccatcgaa ggggctcaat tcgatataca
     1621 tccaggctga tatcggtatg atgacgagcc aggaggagag gatggggatc acccccgata
     1681 tgaagaccag ggcagccagg gcgaaggggt tcggtatccc gaaggcgtag aagaccaaca
     1741 ccgagaggat accgctgagg atcgaggtgt agatggcgcc gatgaatatc ccggagagga
     1801 tactgtctat cctcgcttca tacttcttgt atatcgccag gtgccctgtc gggaggatgg
     1861 tggatatccc cgtggcgagc ttccctccat ccatcaggag gaagtagcag acgtagatcg
     1921 aggcgatgag gtttaaccct atcagaatta tcgacatccc gtatgagaag accgggaagc
     1981 tcgataggat cctcgccagg agctctatca tcgccctcag gcttccggtc agatcgtcgt
     2041 atatagccgg agggatctcg tatatcgccg ggaggatgtc gtatcccgcc aggggtatct
     2101 cgaggtcgcc gaggaaggat cccaccctcc ggatgatctc cgcctggttc tccacgaccc
     2161 agttgagctg ggagatgacc tcgaccccgc ccaggccgag gatgagggct atgggaagga
     2221 ctatcgcggt ggtggcgacg gccgagccga gccgctcgcg cctgccgaac ctctctttga
     2281 tcggccttcc gacgtaggcg aagacgaccc ccaggatgat cccgtccatc agggggaaga
     2341 ggaagtaggt tatcaggagg aggaagaata ggactggaac taggatccag gcgttctcga
     2401 tcagctccgt cctgatgttt ctctccaaag gactctcccc ttcagaggaa cggccgcacc
     2461 gataaataac tttgggggct tgaaatccaa gaagcccctg gctccttttc ccccttcagc
     2521 tccggccgcc cttcggaagc tccaccgtcg ataggaccct atccgctcct tcgacgatct
     2581 cctgctgggt gacgccccgg aggcttgccg cgaggacggc tccgccggcc cctaccccct
     2641 ccttggcgct cccctgggcg tagcagctga ggggcggcat cttcgaccta tcgagccccg
     2701 ggtccgccat gtaccagctc ctcccgaggg tctccaccgt ctcgacgaag ctcgcgttcc
     2761 gatcctccac gatgtacctg gtggtggcga tggagacgtc gccttccagg cccaaagcat
     2821 cggcggcgca taggacggcg gccatctggg tccctcccgc caggaccacc ttcgtccccc
     2881 ccatccccct caagatcccc aggacggcgg ggatcatggg gtcgccgacc tctttcagcg
     2941 ccctcaaggg gtcggacctc atcgatccga aggaggcgcc gcacctctcc agggcggcgt
     3001 cgacgatctc ctgcttcttg gccagggggt ggtcgagaag gctactggag accctgcccg
     3061 gataccccag ggcctgcatc accgccatgg cggtcgtcgt ccctccggcg atcgactcgc
     3121 cgatgaagat gcagtcggag aggctggcga gcttttttcc gagaagggcg gaccggtcca
     3181 tgatctcggc ggcgtcgggg accgctggcg cgaaccggat atcgcttccg ggagagccgt
     3241 tcatgtcgac gtaggggaca gcgggctttc tccgcaggcc gctggtcaca aagagggagg
     3301 ggatccccga gaggctgagg gcggccttcg tcaccaccgc cggggtcgtc gccccctccg
     3361 gggactcggg gatcgcgggg atggtcacga tcttgtggag ctcgacgagc tccgcgtcgg
     3421 cgctgggggt gtagtcggtg aggtcggggg aggcgccggc ggcgctcact ccggggattt
     3481 tgccggtgtc ggtgttggag aggacgcaga ggaagagggg cctctctggc cggtaatcaa
     3541 agtgagggtg gatccagtct cgcatgatca atatggtgtc gtctgggata taaaaactca
     3601 acgccagagg agttaagaca agtaaatttt acttgatcgg cggaaaagcg gggttgccga
     3661 tcgagggtgc cccagaagag atcgcgccat cgatgaagta gggatgcgga tggttggcgg
     3721 acttcttctt ccgccagtcg ttttctccgc cagcagcaaa gatcggctcc accgtatccg
     3781 cccccatcac tcgatcaaat tcacctccac cacctccccc ttctcccgcc cctccagctc
     3841 ctctggcacc aggacgtacc cctcggcccg agccacagag ctgaggatcc cggctccggt
     3901 ggtcatgatc ggcgtcgcga cgccctcctt gaggctcacc ctcgcgaagg tgaggtagcc
     3961 ggggcgcgat acgatctttc cggcgagctt ggcggggaag gttcgcggaa ggtcggtcct
     4021 gtgggccatc atggtgatga ggggccgggc gaagatgtag agggcggcga gggcggcgac
     4081 ggggtagccg ggaaggcaga taaccggctt tccatcgatg actccgaggg cggtgggctt
     4141 tgcgggggtg atccggacgc cgtggacgag gagctccccc atctcctcga tgatcggggg
     4201 agcgtagtcc tcctccccga tggaggtgcc gccggagatg aggatcaggt cggcgtcgag
     4261 gcttcgggcg atagcctctc ggatgaggcg agggtcgtcc tcgacgatgg acgtcttctg
     4321 gggcgacccg ccccagatct cagcgtacat ctcgcagatc agcccattcg cctcccgggc
     4381 ttggccgggc aagagtttct ggccggagaa aagggggacg agttcccccc ccgtggggat
     4441 gatcgcgacc ttcggcctcg cgaagacctg gaccgtcgag aggccgaggg aggcgaggag
     4501 ggctatgtcg gggggcctga gccgatggcc ttcttttaaa accgtctccc cgagggcgac
     4561 gtcctcgcct acccgggcta ggttgcggta ggggtggacc tccgccgtcg cctccaccac
     4621 ctggcctcgg aggagggcgt cctctatcat caagacggcg tccgcccccg gaggcgccgc
     4681 catccccgtc cggacgggga cgcacctccc ctccccggcc tcgtcgccga cctcgaggta
     4741 tactggggcg aggggggtcg cgcccctggt atccgccgcc cgaacggcgt agccgtccat
     4801 ggcggccctg ttgaaggcgg gaaggttcac cggagaggct aggtccccgg agaggaccct
     4861 gtcccgggcg ctggcgagat ggaccggctc ggacctcgtc accggtccgc acctctccag
     4921 gaggtccttc agggactcgg aggcgctggc aagctctttg aacatgtttc cagctatttc
     4981 cgtccaaccc atctttagcc ttccgtcggg atgaagtccc tccatccggg gatcggcggt
     5041 cctatccttc ttcttcttcc gcccctcccc gtgaaaacag atatatacgg tcccgccgcc
     5101 atctccccgc aacaagatcg aacattatag atcgaaattt cgcttcatgg atagaaggtg
     5161 gagatcccat ggctgctgta gtttggctgg aagaagttgg aaaagaagac gtcgccgtcg
     5221 tcggcgggaa gggcgcaagc cttggcgaga tgataaacgt gggcgctccc gtgcccgggg
     5281 gctttgcggt caccgctcag gcttatcgag aattcatcac ccgatcggga atcgcggagg
     5341 gtatattcga ggctctgaag gtggacgtcc acgatcccga agccctcagc ggcgccgcgg
     5401 agagggcgaa gcggctcgtc ctggaggcga aggtcccaaa agagatcgag aaggcgatca
     5461 gggaccggta tcgagagatg tccaagaggg agggaaagga ggtcctcgtc gccgtcaggt
     5521 ccagcgccac cgccgaggac ctccccgacg cgagcttcgc ggggcagcag gagaccttcc
     5581 tgaacatcct gggggaggac caggtcttcg aggcggtccg gaagtgctgg gcctctctct
     5641 acggggcgag ggcgatcttc taccgggtcg agcagggctt tgagcacgag aaggtgaaca
     5701 tctccgtcac cgtccagaag atgataaact ccgagaagtc cggggtgatg ttcagctccc
     5761 accccagcac tggcgaaccc catgtgatca tcgaggcggc ctgggggctc ggggaggcgg
     5821 tcgtcagcgg gagcgtctcg ccggacaact acgtcgtcga cagggcggac aagaagatcg
     5881 tgagcaggtt tgtcgcgaag aaggagatca tgatcgtccg ggacaagaag accggcaaga
     5941 ccgtcaccaa gaaggttccc gccgataaca gggagaaggt cgtcctctcc gacgaggaga
     6001 tcctccagct cgcagcccag ggcgaagccc tggaggagca ctacggcatc ccccaggaca
     6061 tagagtgggc cttcgagggg gggaagatgt acatcctcca gtcgaggccg atcaccacca
     6121 tatcgaaggg cgatggaggc ggggcggaga aggctatggg aagcggaaag gtgatcctca
     6181 ccggcctcgg cgcctctccg ggggtggcga ccggcgaagt gaagatcgta tcgggttccg
     6241 gggacctggg gaagatcaaa gagggggaca tcctggtcac ggtgatgacg gagccggata
     6301 tggtcccggc gatgaagagg gcgggggcca tagtcaccga cgagggaggg atgacctgcc
     6361 acgccgccat cgtcagccga gagctggggt tcccggccgt cgtcgggacg aaggagggga
     6421 ctaagatcct caccgagggg atgatcatca ccgtcgacgg agggaagggg aaggtctacg
     6481 aggggcggct caagacgccg aaggctgcgg aggcggccgc gtctcccgta tccttcgtct
     6541 acaagccgat aactgcgacc gagattaagg tgaacgtcag catgcccgag gcggcagagc
     6601 aggcggcagc gaccctcgcc gacggcgtcg gcctcctccg gatcgagcat atgatcctgg
     6661 ggctcggcaa gaccccccgg tactacatca acgccggcaa agaggatgag tacgtcgacg
     6721 agctggtggg cggcgtcagg accgtggctg aggccttcta ccccaagccc gtctgggtca
     6781 ggaccctcga cgcccccacc gacgagttcc gggggatgga ggggggggag aaggagcccc
     6841 acgagcacaa ccccatgctc ggctggcgag gcatccgaag ggatctgacg gagaccgacc
     6901 acttccggct ggagatgagg gccttcaaga agctccacga gatgggcctc tccaacgtcg
     6961 gtatcatgct ccccctcgtc cagcacgtca gcgagtttca gagggctaag gccctgatgg
     7021 tggaggaggg gctcgacctg gagaagatcg acgtcgggat catggtcgag acccccgggg
     7081 ccgctctcac catcgacgac ttcatcgagg acgggatcga cttcgtctcc ttcgggacca
     7141 acgacctcac ccagtacacc ctcgccgtcg acaggaacaa ccagaacgtg gcatacctct
     7201 ataccgagct ccacccggcg gtcctaaccc tgatcgagta cgtcgtcgag cggtgcaacc
     7261 gggccggggt caccacctcc atctgcggcc aggccgggtc ctaccccgag atggcgaagc
     7321 ggctcgtcga gatcgggatc accagcatct ccgccaacat cgacgccgtg gccaccgtcc
     7381 gggagacggt ggcccgggcg gagcagatca tcatgctgag ggctgcaaga gaggagtgat
     7441 cctgaggaag agacggcgga agaggtatcc cccttgagag atcgaggcct atccgaagag
     7501 gaggtcatga ggggcctccg gaagatgagg gcgaaggatc tgagctacga tcggatcttc
     7561 tcctccatgt gcaccccccc ccacccgata gccctgaagg cgcaccagct cttccaggag
     7621 acgaacctcg gggacccggg gctctttccc gggtctgccg agctggaggc ggaggcggtg
     7681 aggatgatgg cggagctcct aggccatccc gaggcctgcg gctacctctc gacggggggg
     7741 acggagtcga acatccaggc gatccgggcg gcgcgaaact ccgccgactt ccgggatggg
     7801 aacatcgtcg tccctaggtc tgcccacttc tccttcgaca agatcggaga ccttctatcc
     7861 ctcgagatca ggaaggcgga cctcgacggc gacctgaagg tggaggtcgg gtccgtcgag
     7921 gagctgatag acgagaagac ggtatccctg gtggggatcg ccgggacgac ggagttcggc
     7981 caggtcgacc ccatcgaccg cctcgggagg ctcgccctgg actgggggat acccctccac
     8041 gtcgacgccg ccttcggagg cttcgtcctc cccttcctcg gaggagactg gaggtgggac
     8101 ttctccgtcg agggggtcac ctccatcacc atcgaccccc acaagatggg gatggcgacg
     8161 atccccggag gaggcctcct cttccgccac cctgaggacc tggagcggct tgccgcctac
     8221 gccccctatt tgacggtggc gaggcccaag gccctgacgg ggacgaggag cggcgcggcc
     8281 gcggccgcca tctgggcggt gatgagccac ctcgggatgg aggggttcaa ggaggtcgtc
     8341 gacggttgca tggcgctatc gcggaggatg gcctctggcg cgaaagagat cgggatcgag
     8401 cccgtcatcg agccggtaat gaacgtcgtc accctgagga tggaggatcc cgagggggtg
     8461 agggccgccc tcctcggccg ccggtggagg gtctcgacga ccaggtcccc gaaggccctg
     8521 aggctgatca tgatgcccca ctcgaccgcc gagaacgtcg acctattcct gggagacctg
     8581 gaagacgtcc tgagaaagaa ggcctgactt ccgggagaaa gcggaaaaat aatggtttga
     8641 tcagcccttc tcgggctgag atatcttttt gacgtcctct cttgctcaga ccttgtggcc
     8701 gatggcgaac nnnnnnnnnn nnnnnnnctc tggcgatccc gtcgccctgt ttcgcgatgt
     8761 cctcgatctc gacgtcgtac tcctttccga cctcgacagg agctgtggac tcaaagctgt
     8821 cgtcccggaa agaccctcta tcacttctga acaactactt caccttcctt ctggaaattg
     8881 agaatgatca aaacgctcag gccttgtggc cgatggcgaa tctctgcatg accctgtcga
     8941 tgacgaccgt gacgtggtcc ccgaccttgg tgtccgggac gaatattacg aacccctcga
//
LOCUS       DASM01000054            5852 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_11798, whole genome shotgun sequence.
ACCESSION   DASM01000054 DASM01000000
VERSION     DASM01000054.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_11798"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    5315..5372
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1102 a         1740 c         1814 g         1131 t
ORIGIN      
        1 ccgatcgtcg gcgccgaaaa catcagccag accctcacca acgcccggga cgccctccag
       61 tatgtccacg accagacggt ccgcctgatg aaccaggggc tgacccccgg cgagatcatt
      121 gaggtcctcg agctgcctcc ccacctagcc gaggacccca atctgcagga gtacttcggc
      181 gagatggatc gggacatttt ccagatattc cagcaataca tgggatggtt cacgggcagg
      241 agcagagaca tattcccgac atctccgcgc gaagaggctg agaagatggc atacctcgcc
      301 ggcggcgtcg acgggctggc agaaaaagcg aaagcgtctc tcgatgaagg tgatgttaaa
      361 tgggcgatga tcttctcgga ccacgtcctt ctcctcgatc ctgagaacgt cgaggcccga
      421 gaggtcaaga actcgtcgat gctatacttg gccgaggaga ccaagaacgc ccaggtgaga
      481 aactacatcc tctccgagta cctcgaagag acgggccaga tcaggatccc catcgaacgc
      541 ggcttcagcc tcatggacga gaatatcgtc gtctacatgc cgatggagac gatattcagg
      601 atcatggcgg tgagcatcaa cgccaccaag gccctggacg aggagtacgc cgtcggcctc
      661 agcctctcgg acgattcgga atctgcagaa tatgccctgt acgtccggag ggggatcgta
      721 gaggttcagg aggtggtccc cgaggacccg aagttcacca tcgacaccga ctccctctcg
      781 atgaagaacc tggtcctggg aaagctcaac cctgaggagg cggtcgccgc cggagccgtc
      841 gaggtgggcg gagccgggcc ggaggagttc tacgggttca tggacctatt caagtgatgg
      901 gggcggattt caaagccccc gtcggcctat ttttccctaa aggagccttg ccagcagctc
      961 cggacggctt aagactatcg gttctccagc ggtacgtact cccggtcgaa ctccccgacg
     1021 tagagggacc ggggcctgaa gatccggttg ttctcgaggt actccatcac ccttgcggtc
     1081 caccccgcca cccggctgac ggcgaagagg acggtgtaga ggtcgccggg gacccccatg
     1141 gcgtggtaga cgatcccgga gtagtagtcg acgttcggga agatcttctt ctccatcccc
     1201 aggtccgaga cgacctcctc ctccagccgc tttgcgatcg aatacatctt atcgcagttg
     1261 gggtttgact ccgccatcat ccccgccagg ggtcccagga cccgggcccg agggtcgtag
     1321 gtcttgtaga cccggtggcc gaatcctggg accttccgcc ccgaacttcg ggccgtgttg
     1381 taccactctt tcaccccttc cggggagccg atctcccgga ggaccctcgt tacctcctcg
     1441 ttagctccgc cgtggagggg gccgttcagc gcccctatcc ccgcatcgac ggagaagtag
     1501 atgtctgaca tggtggaggc gacgacgaga caggcgaagg tgctggcgtt catcccgtgg
     1561 tcggcgtgga ggatgaggga gacgtccatc accctctcct ccaccggcgt cggccttcgc
     1621 cccgtcatca tgtagaggag gtttgcggcg tggccgagct ctggatcggg ctcgatgggc
     1681 aggcggcccc tcctcagccg accgatggcg gccgccaggg tcgccatccc ggcgatcagg
     1741 tggatacagc tatccatgct ctcggcgtcg tcgggggcca gtctcgggtc tgaaggccgt
     1801 cggaggtgcc gcctctggaa ctcatagatc gcatgcctct cccccgtcgg ccgggtctcc
     1861 atggggatcg agtcctcgtc ggaggctatg gcgtcctcca gggagggcct tgcgtcctcg
     1921 ctgtcccgcc aggtctgcct ctgatgcatc aagttcgtcc ccagcctcag ggctgccatc
     1981 gggttcatcc tctccaccgg ggcgttcatc agagtccgga gggtgttggg gaggaagcga
     2041 tattcgttca gtcggctctc gaaggaggcg agctgccttt ccgtcggtag gtcaccgtag
     2101 aggaggaggt aggagacctc ctcgaaggtg gagtgggcac agagatcgaa gatgtcgtag
     2161 ccgcggtaga tgagggtgcc ttcggagccg ttgacgaagc ccacccggct cttgcaggct
     2221 atcgccccct caaggccagg ccccacggta catcttatcg gccagtcgat ccccctcatc
     2281 tggtcgaaga tgggattcgt ccctttggga gccgcggacg cctcaccttt ggcgcccagg
     2341 atcagctccg tgacatatct cttcgtcctc tccatcgcat catctcctct ccgatccgat
     2401 tcccggcggc ttctcttctg gcaggtggtc tctggccgcc ccgcttcctt ctagccttcc
     2461 cgataaaagc ggctcatcta tccagacggc cgagtcaggg tcggggtcca ccaagcgtca
     2521 aggctacctg atcttcccgg tcggaataga acttccggcc tctcacgatc tgttcatata
     2581 taaccctttc accggcggtt tgaaccttgg gggttcttcg ccggcgggac ctcgatccgt
     2641 cccagggtgg ctatgggagg ttcattcatc cccccatcta atatttgaac aggatcgaaa
     2701 tcttattaga ttaatagggg caataatgac gaggatctgc catcggaggt ctggcagcat
     2761 gacgccttgg tagatcaaaa aataatttgg gtaatcgtga tataaaatca ggctggatgg
     2821 tgttattatg aagatcccct cgacgatgat atccgtcata tgcctcggag ccgccgtcat
     2881 catctccctg ggttccgcct gggggctcga tactggagcg ctttcaggag atgacaaggg
     2941 cgactggtac agatttgctc ctccttcctc cccggaaggg ctctctcccg agggcccctc
     3001 cgccgacgaa ggcggtggcg aaggcatctg gataaccgac ctggacggga aggagaggag
     3061 gacggccctg gagatcccca tgggggcgtg ggcgaggttg atgatcgccc cttcctcgga
     3121 gggggatctc gagctcctca tacgataccc cacgggaacg gtccagaaga tgctggctgg
     3181 gtcggtggag acgggctgga tttattccag ctggctccag gctgaatggc cggggaagta
     3241 cctgatctgg ttctccctgg acggggagga gagcgaaccc gtccagcttg acgttcagga
     3301 ggagctgata gtctttgagg gggctggacc ggcggcgccg gggtcttatg ccgccggagg
     3361 tccggggctt gggggacctg ccaannnnnn ncccctcccg acggacgtca cctacgaggg
     3421 gctcttctac gactacagct ttgagaccgg gcagaaggag gaatgccaga agctcttctg
     3481 ccccacttac agctctgcca tatcgaggga tcccatctct ggccagcccc agcgatacct
     3541 atctgtcggt ctcaactcgg ggatctccga ctttgccaga aagaagctga acctggtcgt
     3601 cgtcctcgac tactccggct ccatgggaag ccccttcaac gaatactact acgaccgttt
     3661 cgggagccgg gttgatgtcg agggggattc ccgcctcagc aagtcgaaga tggagatcgc
     3721 cgatcgggcg gtggtggagc tcttgggcca tctggagggg gaggacaggt tcggcctcgt
     3781 agtcttcagc gacgaggcct acctcgtcga tcccctcacc aggatcgacg ataaggatct
     3841 ggatgccctc aaggccgcca taatcgagat cgaggagtac ggcgggacga acatggaggc
     3901 ggggatgatg aagggaacgg ccgccttcgg caagtatggg gagttcgacc ctgttgaggg
     3961 ggagaacagg atcatcttca tcaccgatgc catgccgaac ctgggggaga cgggggaggg
     4021 tggcctcctg aggatcctcc gggagaacgc ccgaaagggg atttacacca ccttcatcgg
     4081 ggtgggtgtg gacttcaaca ccgagctcgt ggaggagatc tccaaggtcc ggggcgccaa
     4141 ctactactcg gtccactcgg cgaaggagtt tgaagagagg atggacgagg agttcgattt
     4201 catggtgact cccctggtct tcgacctcct cctccggctc gaggctcccg gatacaggat
     4261 agataaggtc tacggctcgc cggaggcgga tgaggctacg ggggagatca tgagggtgaa
     4321 taccctcttc ccctccaggg cagaggaggg ggaggtgaag ggcgggatca tattgatcaa
     4381 gctctcccgg atctccgacg acccgaggat caccctatcg gtcagctacc gggatcggaa
     4441 cgggaccgaa gacggcgccg aggcggcggt gatcttcccc gaggtggagg gcgacttcta
     4501 cgagaacgac ggaatcagaa aggcgatcct cttgtcgagg tatgcagacc ttctgaagaa
     4561 ctggatcatc gatgagaggt cggccctgga ccaggatgct ccggttgagc cctccgtcac
     4621 cctcgaggac gggatcgtcg tccccggacc tctcctgggg gagtgggagc ggcagtcgct
     4681 gcccctgacg gtatcggagg gttatcgggt cttcttcgag ctcttcagga gccacttcga
     4741 gaccgagagc gtagccctgg gagatcccga cctccagcag gaggtggtcg tcctcattaa
     4801 gctgagcagg ggggaggggt gaggatcgtt agaagggata gaggacttag aggacctcga
     4861 tccccataaa cgaaagttgc cgcccccggt ttttttccgc ccgagcggca ccgcccctcc
     4921 aggggaagga gaaggggtca atcccggaaa ggttcccctc cgccctcgac ctcgccgggt
     4981 tcgccgaagg gggtcctcat ctcgtagacc ccatcctcct cccagacctt ctccaccaca
     5041 aagcccatct tctggaacat tcgaaatatc cggacgttcg tggataggac ctcggcgacg
     5101 aacccctgga ccccctgctt ccgagcgagg ttggtgaggc gggcgagaag ctctcttccc
     5161 accccctggc ggtggtagtc gtccctcacg acgtaggcga cctccgccaa catcgactcg
     5221 cccaagaggc cgtactggcc gatcccgatg accgtctccc tgcccgcagg ccggtcctct
     5281 acggtcgcga ggatcaccat cttcttagaa tagtnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgccctcg ccggggatga aggcctggtc
     5401 tccgtagatc agccccatcc tcttagcctc ctttatcagc tgggggcgga agtcggggtg
     5461 ggcgatggct ataaggtcga gagccctctc ccggacgttc ttggcgtgga ggtaggcgat
     5521 cccgtattct gtgacgacgt agtggacgtc cccccggctc aaggtgacgc cggagccggg
     5581 cctcagggtg gggacgatcc tcgatctcct ccccccgtcg gcggtggatg gcagagcgag
     5641 gatcgacctt ccggctggcg agaggaccga tccccgcatg aagtccgcct ggcctccgat
     5701 cccgctgtag tgggatcccg ccagggactc ggccgtcgcc tgccccgaga ggtcgatctc
     5761 aagagcgctg ttgatggccg tcatcctccg gttcttggct atcaccaggg ggctgttggt
     5821 ataatcgatc gacctgaact cgacgagggg gt
//
LOCUS       DASM01000055           10242 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_49161, whole genome shotgun sequence.
ACCESSION   DASM01000055 DASM01000000
VERSION     DASM01000055.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10242)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_49161"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    6747..6759
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2081 a         3047 c         3083 g         2018 t
ORIGIN      
        1 cccccttcaa catcaccgtc caggccctga gggggctccg gttcccggag gtcgtcctcc
       61 agatattcct cttcacctac cgctacctct tcctcttgag cgaggagatg aagtccatcg
      121 agaagtccct cgcctccaag agcttcgcca agaagaccga cctgaggacg gcgagggtga
      181 tagggaccgc cttggggatg ctctttgtaa gaagctacga gcggtccgag cggatatacc
      241 gggcgatggt atcccggggg tacgacggcg ccctcccctc cacggttaag ttccagatga
      301 agagggggga ctgggcgaag agcctcgccg tggcgggggc cgccctcctc ctccagctga
      361 tcgatcacgc cgccacccag gggatgatcc tctgagcgaa tacgccatcg agatccagga
      421 tctcagctac agctaccccg acgggacaag agccctcgac ggggtcagct tctccgtaga
      481 gcgggggagg tcggtcgccc tcctcggccc caacggcgcc ggaaagtcga ccctcctcct
      541 tcacctgaac ggccttttga ggggggatgg gacggtcagg atcctcggcc ggaaggtcga
      601 ggaggagagc ctcccctggg tccgcagccg ggtggggatg gtcttccagg accccgacga
      661 ccagctcttc atgcccaacc tccgggagga cgtcgccttc ggcccccgga acctcggcag
      721 gaccgaggcg gaggtggaga aagacgtctc ccgggccctg gaggcggtgg gcctccaagg
      781 gctcgaaggg aaggcccccc accacctcag cttcggccag aagaagaggg ccgccctcgc
      841 ctcggtcctc tccatgaggc cggaggtctt ggtcttggac gagccgacct cgaacctcga
      901 ccccaagtcc aagaggacgg tcgtctccct cataagggag ctctgcgact cggggacgac
      961 gatcatcacc gccacccacg acgtcaacct cgtgaccctg atggccgacg agatcctcct
     1021 cctggatagg agggtgatgg ggtatggccc cctgagggag atcctcgaag agagggcggt
     1081 cctggagggc ctcggcctgg aggtcccgat cctggtggac ctcttccact ccctgagggg
     1141 ctgctgcagc catcacttcc atcagacgcc catcacaaaa gatgaggcgg tcgaggggct
     1201 gaagaggatc ctcgggtgag ggacctagac ggtcaaaaag aagaaaaaga ggtgaggcgg
     1261 accttcagtc cgcctcgggc ttagtatagt cctgccggag ggtcaggacg gagaagtagc
     1321 ctgttcctac aaagctctcc tcgacggcgt ccggcccgtg ttgtggcaga agcaccatct
     1381 cgccgtcttt atgctccgtt gtcacgatcc agagccccgg ctcggggacg gcgaggacga
     1441 ccaccccctc ctcgtcggtg acccctttcc ccacggatgg gttttcggtc gtcgcaagcc
     1501 tgacctccag acctgccgcc ggctctccat cgaatagtac caggaacctg aagtcgccgg
     1561 tgctgagggc ggtgatgttc tccaggggaa ctatctccag cctcgtgccg atggggacgg
     1621 aggcggtggc gaagtcggcg ttctccgcca ctacgaagag cttggagaac ttcaccaggt
     1681 cttcgtaggc ctcggcgatg taccagtcgg ggtcccagcc gctggtctcg acctcatccc
     1741 aggcgatctc gtcgtacccc tccatctcct catccggggc gccgaatttc gtccggagga
     1801 aggtgagggg gccctcgtgc tgctcacccg tcacgacgta gaggccgggg ctttctacat
     1861 cgaagtcggc tcggtagtag gggccgttcg tcgccgtcgt ctcgtcgagg tcgaaggacc
     1921 gatcgtaccc accggggccg gtcaccctga tggatatatc tccagggggg ggcgccatgg
     1981 ggacgaagga gtggccttcg gtcatctgga cgagggcgag atccccctcg ctcacccaac
     2041 cgctggtggt caaaagccag gtatcgtggg cctgcccacc agcgacggcg aaggccagga
     2101 ggaggcagac cgccaatatt ctgttcattc aaaacacccg tagaccttta ttaatactaa
     2161 atacataaat gctctcactg gatgatcaga aaaataatat tagcaggcac gatatcccca
     2221 tatcggtgat gaagtgcctg taccttttga gggggggtat ccccccaaag aaggtctttt
     2281 cagtatccag tggatagata gtcggatctg agggttagag ccgcccgaaa gaccggacct
     2341 acgtagctct tctccgcgga gaggggtccc ctcgggaggt ccaccgcctc cacgatcctc
     2401 gggtccatct tggtgtccgc cctgactatc cactccgtca gctgtgaccc ctcgtcggtg
     2461 aggttgaggg tgaacttacc ctccccgtcg gtgacgctct ccaccggggt gtcgtccccc
     2521 accttcatcg cccagacgtc gatcccggag atcggctctc cctggaagag gagctggaag
     2581 gcgaagtcgc cctccccgac ggtggagatg tcgtcgaggg gcacgatctc cagctcctgg
     2641 ccgacggcct cgttcgcctt ggataggtcg gcttcttcac cgacggcgac gaaggccttg
     2701 gcgtagcagt tcttcggata ggaccgctct acataccagt cgtcggccca gccggtcttg
     2761 ttgagggagt cgaagtcgat gtcgtaggcg taggcgtgct cccagaagct cccgttcgga
     2821 gggttggtca cgatgttcgt ccaggagccc tcggagtggt agacgtcgac gacgtagagg
     2881 ccggcctcct cggggacgaa gcggtatata tcccagaagc cggagatctc gcccatgccg
     2941 tcgaggactg cctcctccgc cgggccgatg acggtgacgt tcaccatccc ctcggggagc
     3001 tcggtgttgg cggtggcgtg gccgctcccc aggggcaggt acaccacatc cccggggctc
     3061 gcccacccgg atatcggtcg gatatacgtc tcgtggccgc tcgcccccat ggccagggcg
     3121 aggaccagca tcgtcagata taatgcgatc cgtctcaagc tatcaccgga gcccactcgg
     3181 taatactagt atatgattat tatcatcagt tgctacgaat gatgtagaaa tggagaaagg
     3241 ttgggggtgg gatcgagatc cccggaggga gagggtggtc caagcaccgc ccgaccttca
     3301 ggcggtgatc ttggacttgg cgatgtacct gatcaggtcg ggggcgagcc gcgtcttcct
     3361 ctggatggcg gcggaggcgt cctcgatgga ctcgccctgc ttgatgaggt ttccgatctc
     3421 ctcgatagcc tcctcgtcga cgacgtagta ctcgtcgatg tccttccggt ggccccagac
     3481 gtccccctcc aaaaggtcga tcccctgcat ctccaggaag agcttgatcg ccttggacat
     3541 cgtcttgtgg tacgacgggg gaacctggac ggcccggagg cgcgggcagc tcctcatcag
     3601 gtcgaggaag tctacgttgg agggcctgaa ggccaggtgg acgatcctct cgttcgggtt
     3661 cagttcagcg atctcgtctt ttgagctgac aactctaatt ctcatcacaa ttcacctcaa
     3721 caaatactaa gatgtgaatc ttagtattaa tagctaattg tgttgtatac taacgtccag
     3781 atggaactta tgtagccgat atggggctcc aggcatcccc tccgggtgga cggatcatac
     3841 ctttccgacc cgaagagatc ctggtctggg ttccgatctt gtgggattaa aggatccggt
     3901 cgccccgagg actttcaaag ccttttctcc ttcggccgcc ccccccggcg tcggaggtca
     3961 gagataagga ggagaaggag aaggggcggg tggaaatgcg gcagttggag aagagggatc
     4021 ggtcttggaa gggcagagct gccgcgggaa gggtccaggg gccgcctgag gtttcaggcg
     4081 gtgatcttgg acctggcgat gtacttgatc aggtcggcgg agagcttcgt cttcctctgg
     4141 atggcggcgg ccgcctcctc gaggctctcc ccctcccgga tcaggctccc gatctcctcg
     4201 atcggctcct cgtcgacgat gtagtactcg tcgatgtcct ttcggtggcc ccagacgtcc
     4261 ccctccagga gggtgatccc ctgcatctcc agaaagagcc ttatcgccct ggagagggtc
     4321 ttgtggtagg agggcgggac ctggatcgtc cggaggcggg ggcagaccct gatcagttcg
     4381 aggaagtcta cattggaggg cctgaaggcc aggtggacgt tcctctcgtt cgtgccgagc
     4441 ttcgatatct cgtcttttga gctgactact cttatcttca tggtctttca cctttacaat
     4501 tgcatggatg tgatctaagt acttatagct acttgcgatg tatagtaacg tccagatctg
     4561 tttccgggtc gtcagaaggg tagctgcaag ccccgtgact cgtgataaca tctgtcatac
     4621 tatctagaaa aatgggacac attcgcccct gtcttccccg accaaaatag gaccgtcgcc
     4681 ccggaccccc tcgggcggtc cgggcgggga ggtggcatcc ggctcgccct ttggatgccc
     4741 cttccgggtt tcaggcggag atcttcgacc tggcgatgta ccttatcagg tcgggggcga
     4801 gcctcgtcct cttggtgatc tcctcagccg cctcctcggc ggtagccccc cgcgagatca
     4861 tcgcctcgat ctccccgatc gtctcctcgc tgacgacgta gtactcgtcg atgtcctttc
     4921 ggtgacccca gacgtccccc tccaggaggg tgatcccctg catatcgagg aagacctgga
     4981 tcgcctggga gatcgtcctc cggtaggagg gggggacctg gatcgcccgg aggcgggggc
     5041 acctcttcat cagggtgagg aagtcgacgt tcgaggcccg gaaggcgaga tggacgatcc
     5101 tctcgttcgg gttcagctct ggtatctcct tctttgagct aacaactctc agtttcatga
     5161 cctttcacct caacatttgc cccgatgtaa gccccttatt aatagacgct ggtatagccc
     5221 ggcggcctcc gatggaccct ccggcggaag ggacgccctt ggatccgcgg caagggccgc
     5281 gattcaaata tggggccgag gtgggggcag aggtggatcg agatccccct ccccagggta
     5341 ccgaaaaacc ggaggtgatg ggggaggggc ccgtccagct cagaccgtca ccttggattt
     5401 ggcgatgtac ttgatcaggt cgggggcgag cctcgtcctc ttggtgatct cctcggccgc
     5461 ctcctcgacg gtcgcgcccc ccgagatcat cgcctcgatc tccccgatcg tctcctcctc
     5521 gatcaggaag tactcgtcga tgtcctttcg gtggccccag acgtccccct ccagaaggtc
     5581 gatcccctgc atctccagga agagcttgat cgccttcgac atcgtcttgt ggtaggaggg
     5641 ggggacctgg atcgcccgga ggcgggggca tatcttcatc aggttgagaa agtcgacgtt
     5701 ggaggccctg aaggccagat ggactatcct ctcgttcggg ttcaggtttg gtatctcatt
     5761 ttttgagcta acaactctaa ctcgcatgaa ccatcacgtt tggcgagatg aaaacaaaat
     5821 actaatagac acttgtggag gacactaccg tccacgccct tctccggcga cccccgtccc
     5881 ggaagatggt tcgagatccc ctccttcagg ggcggatctc cgcccccctc aagctaatat
     5941 tttgcggcgc agatccccgc cccggggatt gaacttcgca taacaattct gctaagcttc
     6001 ataaccaatt gagagttggc tcccctcagc cccccgcccc ggggccttca aaataatcgc
     6061 cgaggctcgg gggcccaggt cccaaagccc gctccccccc ccaccaagtt taaataactt
     6121 ataataatct ctccccggag aagtggtcca ggctggtaat ccttatgaac gttgacgaga
     6181 tagtccgatg ggttctcagt gcggatcggc gcctcctctt gatgaggtcg atccggaatt
     6241 ccagggtggt taaggcatcg gaagtggctc gaagtactga tcgatcgatc cagaatataa
     6301 gcagggcgat aaaggagtgc gaagagcaga aactggtgga gtgcataacc cccgagaagc
     6361 acacatggaa gaagtacatg ctcaccgacc aggggcatca cgttttgaga gagctagaaa
     6421 aacacaagat gatctgaatt agatggaagg ttgatctcat gggtaaagta aggataactg
     6481 aaacggtctt cagagatgct catcaatcac ttcttgccac caggatgaga acacgggaca
     6541 tgctcccgat agcagagaag ctcgaccagg tcggcttctt ctccatggag gtctggggtg
     6601 gcgccacctt cgactcatgt atcaggtacc tgaacgaaga cccctgggag aggctccggg
     6661 ccctgaagct cgccatgccc aacacccccc tccagatgct cctccggggc cagaacctgg
     6721 tcggctaccg ccactacgcc gacgacnnnn nnnnnnnnna aagatatgga cgcccacgtc
     6781 cagggctgcg tctgctacac cacaagcccc gtccattcga tagagcagtt caccaagatg
     6841 gcggtggagc tcgccgacct cggctgcgac tccctctgca tcaaggacat ggccggcctc
     6901 atcctccccc acgacgccta cgatctcgtg gcgtcgatga aagaggagac ctccatcccc
     6961 gtcgatctcc actcccactg caccagcggg atggctccca tgagcttcct cttcggctgc
     7021 gaggcggggg tagacatcct cgacaccgcc atatcccccc tcggctgggg aacctctcac
     7081 cctccaacag agtcgatcgt cgcggccctg gaggggaccc cctacgagac cggcctcgac
     7141 ctgaagctcc tcaccgagat caagaggtac ttcgagaagc tgatggaggt ctacgccccc
     7201 ctcttcaacc ccatagccgc ccgggtcgac agcaacgtcc tcgtctacca ggtcccgggc
     7261 gggatgctct caaacctcgt ctcccagctc gtcgagcaga aggccctcga caagtacgac
     7321 gaggtcctcg ccgagttccc cagggtcagg gaggatctcg gctacccgcc cctcgtcacc
     7381 cccaccagcc agatcgtcgg aagccaggcg gtcctcaacg tcctcaccgg cgagaggtac
     7441 aaggccgtcc ccaaggaggt gaaggattac gtcaaggggc tctacggccg gtccccgggg
     7501 gagatcaacc ccaagatcag ggccgagatc ctggaggacg aggagccgat caccgtccgc
     7561 cccgcggacc tgataccgcc cgagtacgag aagatgaagg ccgaggcgga agggaagggg
     7621 ctcgtcagga gggaggagga cgtcctcaca tacgccctgt atccccaggt ggcggagaag
     7681 ttcctcaagg gggaggcgaa agaggaggtc ctcaagaagg ctctccctgc agatctccag
     7741 accggggcca tcggtggaaa gcccgcggcc ttcaacgtcg aggtggacgg cgagtcctac
     7801 ctcgtcaagg tcgctccggc ggggatctcc atcgaggcgg tggagccgaa ggcgcccaag
     7861 gacggggtga cggtcccgat gcagggggtc ataatccgct acctgatcaa gaagggcgac
     7921 aaggtctcca agggggacgc cgtagccgtc ctcgaagcga tgaagatgga gaacaaagtc
     7981 tccgccaaca aggacggcgt cgtcgcggag atctacgccg aggtgggcgc caccgtcgcg
     8041 ccgggcgaca tattgatgtc catcgactga gatcctcggc caggttcgga tgaaaaggaa
     8101 ggatcagcgg atgtcggtca aaagggaaca ggtgctcatc gaggctctcc cctacctgcg
     8161 ggagttctac gactcgatca tggtgatcaa gataggcggg tcgatcatgt ccgataggga
     8221 ggtgctggac accttcgtcc aggacctcgt cctcctccgg tacgtcggga tccatcccgt
     8281 cgtcgtccac ggcggcggcc ccgagatctc cgagaagatg gcccggttcg gcaagaagtc
     8341 cgtcttcgtc gggggcctgc gggtcaccga cgaggagacc ctggagatcg cgaggatggt
     8401 cctcctcggg aacatcaact ccagcctcgt caccctcatc ggaaagcacg gcggcaaggg
     8461 gatcggcctc tccggaaaag acggccagct gatcgtggcg cggaagaagg cgccgatcgt
     8521 catcgccgga gaggaggtgc ccatcgacct cggctgggtc ggggaggtcg tgagggtgaa
     8581 cccggagatc atcatgatca ccgccgggaa ggggtacatc ccggtgattt cccccatagc
     8641 cgtcgacggc gagggaaaca acctcaacgt caacgccgac accgccgccg gggtgatcgc
     8701 catcgccctc ggcgctaaaa agctgatctc ggtgacggac gtcgaggggg tgaagctgcg
     8761 gcccgaggac cccaacaccg tcatctcaag ttttcgcata tccgacttca ggaggatggt
     8821 cgacgagaag gtgatcaccg gagggatgat ccccaaggtg gaggcctgcg tccgggccgt
     8881 cgagggaggg gtccagtccg cccacatcgt cgatggcaga aggccccacg ccctacttct
     8941 ggagctattc accgacaccg gcgtcgggac gatgatcgag aggggttgag ccctctcggt
     9001 cggacctatt ttctttcaac cttcggcggg cggcggcgac gatctcgatc accttgagcc
     9061 ccttgaagca tttctgattc tgtctgctct ttttgctgta ccatctcagc tgatcttcca
     9121 gccggtcaag gatctgtttt tccgaggtca ggatctcacc ttcgggattt tatgaagcgc
     9181 cccatttctt ctatattcat gggctcacca ccttaaaatt atgttgccag atgatggcac
     9241 gagagggcgg ctgtcgaatt ggtgagaaga gcttttggac aacaattctt aagtattaag
     9301 aaagaagaat taaaagagaa gtggttttgc atgacatctg aggagaataa ggccatcgtt
     9361 cgcaggttca ttgaagccta taacaatcga aatttggatt tatttgacga gctggtggcg
     9421 cccgattacg tcgatcacgc ccatcaacag cagggcctgg agagcttcaa gaacctcttc
     9481 accctggcct tcgagggctt ccccgactgg catgagaata ttgaggatat catcgctgaa
     9541 ggggataagg tctgggtccg ggtcgaagcc acggggaccc atacgggcga atgggatctt
     9601 ttcggagtct cactcccccc cagcggcaag aaggtgagga tgatgatggt cttcatctgg
     9661 cggataatcg acggcaagct tgcggagggc tgggaggtag acgacgagct ggatttcctc
     9721 gagcggctgg gtgtaatcga gttcactgaa aaaggaaaga agctcttttc ggaggatgcc
     9781 acgtagaaaa tgtatccacc caaggcaatg acgtggtggt agcgacttca taatcggcga
     9841 gaactgagac aggcctggag gtcttcagag gcgaggccag gatagtttcg caggatctcc
     9901 gcctcggacc acccccgaaa gagaaggccg atgacgaact cgacggccag gcgcgtgcct
     9961 ctgaccaccg gcctgcccgc gagaatctca ggatcgacca cgatccgatc ttgccagatc
    10021 atctcatatc acccttcgtt atttgatcct catcttatct aaataaattg gttctaagct
    10081 tcggtaaagg ttaattcgtt accggcatat accatctcag aagaaacaga gaatagcaat
    10141 agttggtggc agatcaatga agctaatcga gatcattgtg gagggtaagg gctcggccct
    10201 ggccgagatc gacggccgta acccccggac cgctgcggag at
//
LOCUS       DASM01000056            3102 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_115141, whole genome shotgun sequence.
ACCESSION   DASM01000056 DASM01000000
VERSION     DASM01000056.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_115141"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    783..826
                     /estimated_length=44
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          689 a          874 c          957 g          538 t
ORIGIN      
        1 aggaggatgt agaactcgtc ctgggcgggg ctgtccttgt acctgctcag acctgccatc
       61 accacgatga gggcgactgc gacgaagacg agcttgaaga actgggagag ggcgtcgacc
      121 tccagcgtac cgaagatcag ggggcctttg gtcatgtcag cgcccatcac caggaacaaa
      181 gaggcgacca gccccgccag gctcaggtat cccatcaggt tcttgctctt catgaacagg
      241 ccgatgagga ccatcaacag cgagagcccc gtaagcagag cctctgcccc gaagatagag
      301 agatagtcac cgaatgtcag tgtcacttca gatcaccccc gcctgagcga tagcggtaaa
      361 cggatcgacc agcggctggg ctgccacgcc catgatgtcc gtgatcgtcg ccggctggac
      421 cccgaagtag attatgagca ggatgaggat gctcatggag aggatctcgt aggaatgagg
      481 atctttgaga tccaggtact tcttcaggat cggcccgaac atgaccttct gcatcgccca
      541 caggtggtag cctgcggtga cggcgatgcc gccgaagatc actatcagga cgtagacggg
      601 gaggttctga tacgccccgg ccaggaccga gagctcggca acaaagccgc acatccccgg
      661 aaggccgaga gaggccatga acccggccag catcaggacc gcgaacttcg gcatcctatc
      721 ggataggccg ccgagctcgg agatgatcct ggtgccagcg gtgtgctgga tcgtcccggc
      781 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcga ccatcttctt
      841 caggtccttc tgggccagcg ccaggaagcc gccgtaaatt atgctgatga cccctatcac
      901 cgccatcagg gtgatgtagt ggttggagac gttcgggagc atcggcatca cgatcctgaa
      961 gaggccgtag cctcccatct tcaggaggag ggctgccagt agcacggacc ccgccgtcgg
     1021 cgcctcgacg tgggcgtccg gcagccaggt gtggaagggg accgcgggca tcttcaccag
     1081 gaacccgaag aggagcccga agaagatcaa gttcaggagc atcggcgaga agatcgtcga
     1141 atctcccgtc gcctggagga gggtcatcat gtcgaaggtc ctcatgccct caggcgtcct
     1201 gtaactgaag taaagcccga atatggataa gagcattatc aggctcgcca cgtgagtgta
     1261 gatgaagaac ttaatcgcag cgtagtcctt cctcggaccg ccccatatcc ctatcaggaa
     1321 gaacatgggt atcagcacga cctcccagaa gatgtagaat aggaagaagt cgagggctgt
     1381 aaagaccccg aggaccccca cctcgttgag gaggaggagg gcgaagaact ggttggacct
     1441 cgtcttctcg ccccaggagt ataccaccac gaggatgctc acaatggtgg agaggaggat
     1501 caaggggagg ccaatgccgt cgacgcccac ggtgtacttt acgcctaggg cgggtaccca
     1561 gtcgtagctc tccacaaact gcatagcgtt cgaggtcttg tcgaactcct gaaacatctg
     1621 tagggatacc gccagaggca ccagagaccc gagaagggcc acgtacttcg cccccctccc
     1681 ggcgaagaag gccactaccg ctgccaggag aggtatggcg atcatcaacg atatggcgtt
     1741 atccatcata tcgccacccc catcgacgcc agctgaataa gtatcagcag gaccgcaact
     1801 cccaggacta tggcggtgag gtagttctgg atcaccccgg tctgaagctt gcggaccgtc
     1861 ccgccgattc cgacggatat ggcgctaatt ttgttcaccg ctccgtcgat gatctttagg
     1921 tcgatcatgt tggagacgat ggctatgccg tagaccacct tctctgcgaa ccagagggtg
     1981 tagatctcgt tctggtagta cctcttgagg aggatcttcc ggaggggatc cctctcagag
     2041 gtgaccatcc ccggatctaa cctcttccac cgcgagtaga agagggctgc gacgatgatg
     2101 ccgccgactc cgatgatgat cggcaggagc ttgaagatca gcggctcgtg gacgtgagca
     2161 tgggagacgt gatggccccc taccaccgcc agctcctcga agtctacccc gaagtgatcg
     2221 aagttgtttc cgacgtagtg gtagaacccc tcctgggaag gagcccccat gaagagggcg
     2281 aagatcgcca ggatcaccag gggtcccagc atgatccagg gggactcgtg cttgtggtag
     2341 tcgctcctcg cctcgccgtc gaaggtgagg aaccagagcc tgaaggtgta gatcgccgtc
     2401 aagagggcgg cgaggatcac gaagacgtac gggatccatc cgaggccgaa ggctccgttc
     2461 tcgaaggcga ggatgataat ctcgtccttg ctgaagaacc cggtggtcag ggggaaccca
     2521 gccagggtga gggagcccgc tagcatcgtc aggccggtcc acttcatgtg cttcaggacc
     2581 ccacccatct ccctcatgtc gttggtgccg acggcgtgga tgacgctacc ggcgcagagg
     2641 aagaggaggg ccttgaagaa ggagtggccg atcaggtgga agatggaaac gcccacggcc
     2701 gacgccccta cggcagcgcc gaaccccagg gctgccatca tgtagccgag ctggctgacc
     2761 gtcgagtagg cgaggaccct cttgatgtcg aacatcacca ggcccatcgt tgccgcgaat
     2821 agcgccgtaa aggcgccgac gtatgcgacg acggtgaggc cgttgggagc cgcatagaag
     2881 agcgggaaca tcctcgccac caggtagacg ccggcggtta ccatcgtcgc ggcatggatc
     2941 atcgccgaga cggtggtggg accctccata gcgtcgggaa gccagacgtg cagggggaac
     3001 tgaccggact tgccgacggc ccctccgaag aggaagatgg cgatccaggt gagctggtct
     3061 ggtggtatca gggtcaagct gtcgtatatc gtctggaact gg
//
LOCUS       DASM01000057           10871 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_74008, whole genome shotgun sequence.
ACCESSION   DASM01000057 DASM01000000
VERSION     DASM01000057.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10871)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_74008"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1988 a         3457 c         3377 g         2049 t
ORIGIN      
        1 aaccccgccg tcctctcgga ggcggggctc ccttacggcg ccgtcctgac cgccaccgtc
       61 gtagtcagcg ccttctccag catcctgatg ggggtctacg ccaaaaaccc gattgttgtg
      121 gctccgggga tgagcatcaa ctacctcttc gcctacaccg tggtgaaggt ggaggccgtc
      181 ccctgggaga cggccctcgg ctgcgtcttt tgggccgggg cgatcttcct cctcctgacc
      241 ctcctcgaca ggaggaagct gatcctcgag gccgtccctc cgatgctccg gtacggcttc
      301 gccggaggga tcggcctctt catcgcatcc ctgggcttcc agtccgcggg gttcgtcctc
      361 cccctggaga gcggggtaat gggctggggg aagatcaacg ccgctaccct caccttcctc
      421 gccggcttca tcttcaccgc cgtcctggtg gtgaggaagg tccggggaag cctcatcctg
      481 gggatcggag ccaccgccct cctcgcctgg gggatggagg cggccctggg gatgggcccg
      541 cccttcatcg cgagtggatg gtggtcctcc cccgacttca gcctcctcct ccggctcgac
      601 ctctggggtc ccctgaggat cgccctctgg cccatcatct tcgtcttcct ctcgtcctgc
      661 ctcttcgaca gcctcgccac ctgcgtcggg gtctgcgagg cgggaaacct catcgacgag
      721 gagggggacc ccagaaacct cggcagaagc ttgaaggcga acgccctggg ggtcatcgcc
      781 tccgccttcc tcgggaccag cccggccacc tgctacgtcg agtccgcctc aggggtccag
      841 gaagggggga ggacgggcct ttcggcggtg gtggcggggc tcctcttcct gccgttccta
      901 ttcctatccc ccctcctggc cctcgtcccg cccctggcga cggccccggt cctggtcctg
      961 gtgggggtct tcatgctgag gcccctcgtc cgggtccgct gggagaggct cgacgacgcg
     1021 atccccttct tcctggcgat gttcctgatg cccctcacct actcgataac ccaggggata
     1081 atctgggggt gcctcagctg gacagtcctg aaggcggcct gcggcaagag gggacaggtg
     1141 cccccggcga tgtgggctat ggccctcctc tccctcctcc tgctgataga cctggagcgg
     1201 ttcgttcatt gaaagggtgc ggtcggggat gagattgaag gacgggccga tcgaggatca
     1261 cgtcaggctt taccgcgacc tcctgaggag ggggcccctc aggctccgcc tcctggaggg
     1321 ggggcatgcc aggatggggt cgagcctcca tcccctggcc ccctccggcg aggtggacgc
     1381 ctccgccttc ctctactcag cgaggaggct tcccgactgc atggcggagg tcgagagggt
     1441 ggtgatgggg gacggcgacg aagccctccg atcctcgggg ctgatcgagc cctccctctg
     1501 ccggcatgtc cgggccctct cccggcgccg cctgaccctc ttcgacgggc gatcgatcct
     1561 tgccgccgcc atatccagcc cttcggacct cgacgacctc gtccccgccc tcacctgcta
     1621 tcagatggag tggaacaaga tgcacgcacg gctcgccggc tccgacctag gaagggggat
     1681 atcgaagagc cccgagctcg gctcagggga ggaggcgaga cgggtgctga ggatatcgag
     1741 ggaggactgg gagatgatga cgaagacctg gggggaggga tggagggaga agctctcagc
     1801 catcgccagt ggacccaagg atatcaccct cgagctcgcc ccggccctcc ccggcggtgg
     1861 ccgggtcccc atagcctcct ggctggagga gctcctccta cattttcggg atttgggggc
     1921 cagaggtcgc cccgtctact tcgtctccag caatgaccac agcctcccga acctcctctc
     1981 cggatttgtg gccgataacc gggaggagat cctggctttc gccctccggg acccgcggct
     2041 caggaagagg tgggagatcc tccgagacga cgaccccgcc ttcgtcgacg acctcctcta
     2101 ctactccgcc cagggatatc tccggtccgg cgggaggctc tccgccgccc tccgatccgc
     2161 cgaggagggg gtggggatcc ggcggtacga gaggtcgagg ttcttcgacc tccgggctca
     2221 gatcgtcgag atcgggaggc tcgatcccga ccggatcgac gggcggctcc ccgtcagggg
     2281 cgcctccacc ctcgccgaga gccgggccct gatcctgaac gtcgactacc ccctgggcct
     2341 cgccgcccac cgcctcctct cggaggttat cctcgccctc ccggacctcc ggggagtcta
     2401 cgtcatgggg aaggcggcga cgatgtacgg ccgcctgggg gacgtgatga tcccgaccgc
     2461 cgtctgggac gtccactcag gacggctctt ccagttcgag aactgcttca cccttacaga
     2521 ggtgaggggc catctccggg aggcggccgt attcgacgac cagaaggctg tgacggtgag
     2581 gggcaccttc ctccacaacc gggagatgat ggaggagttc aaacgggaag attacaacag
     2641 catcgagatg gaggcgggcc cctacctctc cgccctccgg gaacggatgc cagggaaggt
     2701 cggtggcggc gagatctcgg ggcgccctgg cgggggcaat ctggacctgg gaatcctcca
     2761 ctacgcctcc gacaccccct acagccagag ggtgagcctc ctcagcaaga gcctcggata
     2821 cgaggagatc gaggctacct acgcctgcgc cctcgccatc ctggagagga tcctgggggc
     2881 ggaggccgac cggctccgga ggagataagg gaggcatggg gagagggaca gctgcttatg
     2941 agggcggacc ttgccggacg agtccggacc ggggacacgc ggcagagccc aagcccgccc
     3001 atctggatga acaggttgaa agggtgagag gacctggagg aggagcatga gaatgatgag
     3061 cttgagatta ccatcggcga tcctcctggc ctgcctcttc gtcgccggcg ggatcggcac
     3121 cagcaacccc tccgacctcc cggaggatgg ggggaggatg agcataaact tcagcacctg
     3181 gcacctccac gacagcctcg aggtccagac cgtctggctc ccggtcctcc aggccctgga
     3241 ggagaggagc ggcggcagga tctcctgcac attgatggac ggcggagccc tgggggcggg
     3301 gcccgatcac tacgagatcg tcgcctccgg ccgttccgat atggggtacg ccaccctcac
     3361 ctggaccccc gggcggtttc ccctatccga cgtcctatcc ctcccggcga ccatcgaggg
     3421 gaaggagacg gcgacggaga tcggaagagc cgtctacgac cgggccctta ggggggagtt
     3481 cgccggcgtg aaggttctgg aggtgaaccc ctgcgtcaac tcccacctct ggacgaatga
     3541 gccggtcgag acgatcgaag acgtcgcggg tctgcggatc agaacccccg gcggcctcca
     3601 gaccaggtgc atcgaggccc tcggggcggt cccggtcttc atccccctcg acgccgtccg
     3661 ggtggcgatg gagaatggaa cgatagacgg gatcgtcacc tgcccctcca tgatccagcc
     3721 cttcggcctc gagggggtcg cccgccactg caccctcgtc accttcggct gcgtcggcga
     3781 ggggctcttc gtgaaccagg acgcctggga gaggaccccc gaggatctca aagggatcat
     3841 cgaggaggtc tgcgaaaacc cctaccgtac gacgggcggg atgacgaaag ggacctacga
     3901 cagtatcatc gctgagctga acgagagcgg ggtcgtcttc accaccctcc cgcccgagga
     3961 ggaggcgcgc tggcacgcgg ccttctcgaa cgtgacccgg gagtgggtgg cggagctgga
     4021 ggctcgtggc cttccggcaa gggaggcggt ggagatcttc aacgaggagt gcgagaagcg
     4081 cggggtcgtt tgcgtcgcct tccccccgga gtggaggtag gggctggaaa ggatgagaga
     4141 ttatctctgg gtccctatcc tggatctgca ccgggacccg agatctgtcc ctttttacga
     4201 gattcaggat ttcgtcgcag gccccctgcc ctccaggtag atccctgccc ccagccacca
     4261 gccgtcgtcc accttcgtca cgtagctcgt cttcggctcc tccccgccgg tgagagggtt
     4321 ggtccagagg tactcgaaga agccgctccc gtcttcagcg atggacctca tctcccggtt
     4381 gatgcggacc ccttctgagt tctgaaggtc gaggcggttg gtcccgaccg ctgaggggag
     4441 gaaggggaga gcgaggctcg tcccgttgaa atctgcggcg aagatgtaga cgtctctccg
     4501 cacgaagggc ccttggaggt ccatgaagtc cttcgtcgcg agatccctgc caaagatccg
     4561 ggcgaaggag gccgcagcct cgacgaaggc cgccagctcc tctttcgttg ctggtggctg
     4621 ataggacgaa gatgcgcctt cggctccgaa ttggtcgccc tcagccggat agatccccga
     4681 gccgagccac cagtcgtcgt cgacggccct cacgtacccg agcttgatgg cgggggtcgt
     4741 atccctcgcc ggatcgagat acatgtagta gacgaggccc gttccgtctc tagccacctc
     4801 tcttatgccg cccagaccgg tttcaacgga gggatccatg gcgaacccgt agatatccag
     4861 ccccccccgt cggaccttcc ccaccagatc cggctggaag gggtgggcta ggacccggtt
     4921 ctcgtcgaag tcgtaggcga agaggtactg gttgccgtct acaaagctgc cgttcttgtc
     4981 gttgaacgcc tcaagagccc tctttctctc gttctcccgg gcgtaatcga ccgcgccgtc
     5041 gacgaagctc accagatcga ggcgagcatc ggcgctgaag accgccgaaa cgtcagagag
     5101 ccatactcca gttccgagcc accagtcgtc gtcgaccctg gcgatgtagg agagcttcag
     5161 ctcggggctg tcgtggtgga gggggttggg cctgacgtag taggcgaagc caccgccctg
     5221 agaggccagc cggttgaggt tccggacgaa gagaaacccg ttctcatccc tgaggtcgat
     5281 ctggtttttg cccaccgacc ccggcctgaa gggctgggct agacaggtgc cgttggagtc
     5341 gtaggcgaag acgtagcgga ggtctccgac gacgaactgg cccctggggt cgttgaactc
     5401 ctccagggcc ctctcccggc cgttctcccg gacgtactcg agggcccggt ctgcaaactc
     5461 cacgaggctc cgggactctt cggggaggtc cgtcaggtac atcccgccga cctcccccgc
     5521 ctccgccgac ggtaggggag cggctccggc cgagaggacc gccatcacaa gaaaggtgaa
     5581 ggataaagat ctggcctgca tgaagatgct tacgatttcg gcgtatatca ataatccgga
     5641 tattcttctc ccgcctcgag ggtgcggggc cgaagactca gccttttttc ctcttcagtt
     5701 gaggggcgat ctttaaggca tttctcatac taattttaat tgtattccgg ggcgggtcca
     5761 gccgagccga ggggttaatt ccgcctggat ggagagacgg cgccgatgaa tacgcttata
     5821 tggcaacaga tccaataacc tttgcggaga caatttgatc agcttttcgg aaggtgaacg
     5881 aatatgggaa tgattaaaga gttcaaggaa tttgccatga aggggaacgt cctggacatg
     5941 gctatcggca tcatcctggg attggccttc ggaaagatcg taacctccct cgtcaacgac
     6001 ataatcatgc cgccggtggg gatgctcctg ggcggcgtcg acttcacaaa cctattcata
     6061 tccctctccg gatcctccta cgccaccctc gccgacgctc aggcggccgg ggcgcccacc
     6121 ataaactacg gcctcttcat caacaccatc atcgactttc tgatcgtcgc cttcgtcata
     6181 ttcctgatgg tgaggcaggt gaacaagatg aagaggaaag aggaggctgc acccccgaac
     6241 accaaggaat gcccctactg caaggagacg atccccctgg ctgcgacccg ctgctcccac
     6301 tgcacctcgg agctcagctg aggatcttga ggaccggaga tgtgggggcg gttctgggga
     6361 acggctgatg cgtgggttgg ccccccatcc cccggcgagc aaaataattt tttcacccac
     6421 cccatctccc caagggatgg gggatgggga gcgtttcaaa gatcgtgaat ggggacggca
     6481 ggcaattgga ggctcaatta gagccgggac catcatactc cgccccagga tccattccgt
     6541 ctcgatagag atttatacga aagttcataa tgttcgatcg ttatctttaa taacgtggta
     6601 gtatgaaacg tgctatcact taaacgaggg agatcttcga gctttcggta tttggtgata
     6661 ccatgttggg cataaacgat ccgtggatcc tgggggtcta tctcctatcc gttctgagcg
     6721 ctctcctctg cgtcggctac ggcctcatca actggaaccg gggcggagag aaggaacttg
     6781 aggaaattcg tgatgaagtt tcctgggaga agggggagaa gtcgatggaa gagagggagc
     6841 tggggctgtg atctgaatgg acataatact gctcaacatc atcgttctgg tctacatcct
     6901 ggcggtgggg tacctgggtt accggggctg gaaggagacc tgcaactcct cagactacat
     6961 ggtggcgggg aggaagatcc acccctacgt catggccatg agctacggcg ccaccttcat
     7021 cagcacctct gccatcgttg gcttcggcgg cacggcgggc ctcttcggga tggggctact
     7081 atggcttacc tttctgaaca tattcgtcgg catcttcatc gccttcgtcg tcttcggcaa
     7141 aaggacccgg aagatgggct acaacctctc ggcgatgacc ttccctgagc ttttggcgag
     7201 gaggtatgag agcaggttca tccagtactt cagcggcctc gttatttttc tggggatgcc
     7261 gatgtacgcc tccgtcgtcc tgatcggagc tgctaggttc atcgagacga ccctagcgat
     7321 agacttcgac ctcgccctcc tcttcttctc cgtcatcatc gccgcctacg tcgtcctcgg
     7381 gggcctgaag ggggtgatgt acaccgacgc cctccaggct acaatcatgt tcatagggat
     7441 ggccttcctc ctggtgatga cctacagcca tctcgggggc gtctccgccg cccaccaggc
     7501 cctcacagac ctcgcccccc tcgtccccgc gaacctcgcc gccatgggcc acctgggatg
     7561 gaccgccatg cctgccttcg ggtcggcctg gtggtggacc ctcatatcga gcctcgtcct
     7621 gggggtgggg atcggggttc tggcgatgcc ccagctcgtc gtcaggttca tgacggtgaa
     7681 gtcggggcgg gagctgaaca gggcggtcct catcggcggg atcttcatcc tgatgatgac
     7741 gggggtcgcc ttcgtggtgg gggccctctc caacgtctac ttcgtccagc acgacagcgt
     7801 aatagcaata gcatccgccg ccggaaactc cgacagcata atccccgtct acataaacag
     7861 ggctatgccc gagtggttcg tctacgtctt catgctcacc ctcctcgccg cggcgatgtc
     7921 gaccttgagc tcccagttcc acgcccaggg gacggcggtc ggaagggacg tctacgagac
     7981 gctggtgggg gccaggggag gacgatccat tctgataacc cgcataggga tcatcatagc
     8041 ggtggtggtg gccgtcctcc tcggatacct cctccccccc agcatcatcg ccccggggac
     8101 ggcgatcttc tttggcctct gtgccgccgc cttcctcccg atgtacacct gcgccctctt
     8161 ctggaagagg gcgacccggg ccggagcgat cgcggggctc gtcaccggga ccttcgtcag
     8221 cctcttctgg ttggtcttcg tccatcagaa ggaggcaatt cccctgggga tcgccaagat
     8281 gatcctggga agggacgtcc tcataccatc gatgccctgg cccgtcgtcg acccgatcgt
     8341 ggtggccctg cccctggcct tcgtcgcaac cgtcgtcgta agcctcgcaa cgaagcctcc
     8401 cgaatccgag cacctcgata ggtgctttga ggggatcaag aagtgatccc ctcccaaatc
     8461 aaatgaggaa acaaaatcaa atgaggaata aatagagcta gagctcctcg cccttgacgt
     8521 aagccgtccc cagagccaac gccacgatcc ggccggcttc ccccttgacg cggatctcgc
     8581 agctggagat ccgcccctcc cgcttgatct ccttcgcctc cgcccggagg atctccccgg
     8641 gtgagggcgg ggcgacgaag gtgatctgga cgttgagggc gagggcgacc ctcccccggg
     8701 agtttccggc ggctccgaag gcctcgtcga cgagggagaa gagggctccg ccatgggtca
     8761 tccccaggag gtcctccatc ccctcggtgc agtccatctc cgtcaccgcc cagccggggc
     8821 cgagatccac aagccttatc ccgaggatcc gggagtaggg ctcccccgcc acccgccccc
     8881 tgatcccctc caacgggtcc tccaaagtca tcccctcttc gaccattccg aaccgatctc
     8941 cattcgacca actgctctcg ggacccctcc gccctcaccg accctatctg acccccctta
     9001 tggtgagggg ataacgataa gcttcgccgt tgctcgctct gatcgccgct atgatggcga
     9061 agatgacgga gaaagccagc aggcctaaga tcaggaggag gcccaagatc atggcgaagg
     9121 gcatggccat cgggttccag aagtcgatca tcccgggatg agcggcgggc cagaagaagg
     9181 caatggaccc gaccgagaag aagaccagga tccccgccag gacccagtag agggtgacgg
     9241 agagctggaa gttgagggcc tccttcccct ggtcgtcgac gaaggtgtgc tggtttctct
     9301 tgacgagcca gaccaccaga ggccccagga cgttccccag ggggaccccc aggagcatca
     9361 agagggcgga gagatggcag aaggtagccc aggtccgggc gtccctgtcc tcttcgatca
     9421 tggtaaaatc ctccctagaa attaatatct ctggatatac taagatcttg cggcagcggc
     9481 tcgaatcgag gcacggcggg gtctggacgg gaccatctca tcgccgccct tccttgacgc
     9541 agaggtagct ggcgatgaac cccgcccgat aatcggccct ctgaatggag aagcccgccc
     9601 tctcgatcat cccctccatc atccagccga tggtggagtg ctcgtctctg atgtgcacca
     9661 ggatgtcccg gggggtcccc tcgccgccca tctccgccat ccgcaagagg tagcggtcga
     9721 agaagccctc gtagccggcg gcatcgaagg agtagacgac gtcccggatg aagagctttc
     9781 ccgcaggctt cagcatccct gctatccgga tcaatgccac ctgcttccag aagtcgggga
     9841 ggtgatgaag ggcgaactgg gtcacgacgg cgtccagggg ctcgccctgg tgctcgtagg
     9901 tgagaaagcc acccctgacg tactcgatcc tctcgaagtc ctccgtccgg ccctgccccc
     9961 gaagccggcc tatcgccttc tctctcgcgt atcggagcat cggaagggag atgtcagccg
    10021 cgatcaccct ggagcaccgg ctcacggcgg cgagggaaaa ctcgccggtg ccgcacccaa
    10081 gctccaggac cgtcgcctcc ggcgagaggt cgagaaggtc gaggacctcc ccgacctcct
    10141 ttgccacgtc cctgatcttc gccatcctcc gatcgtaggc ccggatctgg gagaggtcct
    10201 cgtagtcggt cccgatctgt cgagtctcgt cgtaaagcca ggctggatct ccactcatca
    10261 taaccctccg ttgcgccttc caccctttaa ccgatctcga tggtttaagg aggattcggc
    10321 cggtagactc gcttgagaag aagcctgaaa taaaataagg ccaggatctc cataacttca
    10381 ggcggaggag acgaggagga gagatatgtt cgacgacgtc gatagagcat ggaagaaggt
    10441 ccgggccgca gttatcttct cttttgttgc agctcttttg ggccaggcgg caactcctgg
    10501 gatggcggat gaggagccga aagtccccga ttggatcgag gagataaatc cggcagacta
    10561 cgtcctcctg gactctgacc ttcccgccga cctgcacgtc gcgggcgata taagcgccga
    10621 ggcccgggcc gccgaggcgg cggtcatgaa ggacgggaag gccgacgtgg agaggttcgc
    10681 cttcgtcgag gtccacgaga acatttgcca gcccgacccg gagggggtct acgactgcat
    10741 cggcggaaac tacctcctct ccgtctacgt ctacgagacc gcgactcagg ccagggccgc
    10801 ctgggagaag acgatggtgg acgtttatgg cgatcttcct cctccagagg ggttcgaata
    10861 cgtctcgaac g
//
LOCUS       DASM01000058           20686 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_24167, whole genome shotgun sequence.
ACCESSION   DASM01000058 DASM01000000
VERSION     DASM01000058.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20686)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_24167"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         3812 a         6584 c         6447 g         3843 t
ORIGIN      
        1 ttcaacatac ccgtgttcag catcttctat gttcgtattc tgaaatgtct taacgctgtc
       61 atctgcttca gagacctgaa cggaactcaa ggtgccctga ccgactgagg catgggaaat
      121 agccctcgtc ccatcagagt cactagcata tgattcaaca tacccgtgtt cagcatctat
      181 attcgaatct tgaaagacat aaacatggtc attaattcca attatttgtc ttgaatccat
      241 tgacccaaac agggacacta atctttgatt ggcgtttttc agtcccccca attcgccgcc
      301 aaaagcgatt tcgctttttt cagcatcgaa ggatgatacg tcctgatcaa cagccagagc
      361 cgctggagtt aagtaggcat tgctgtttat cagcgtttca gatccatctt ggatatagat
      421 cccattccag gcaatgtaat catcactgcc gctatacctc tgaaatacgt caatatcacc
      481 aggacctgaa atactcctgg tatcagacat gcttacgccg tcatcaaaac tagcggagga
      541 ttttccgctg acctcacaac tgtcgtcgac atcgtaatga tcccagatgc tcacgtaact
      601 tccgccaccg ccgctcgccg aaaaatgtat gtcaattgca ttggcactgc caccacacac
      661 caaaaggcaa aatgttataa ttaatacgtt atatattcta gacattatat cactcctgga
      721 gaccacttgt ctagtgttga tcctctaata actctgctat atatgtttgg tggtgcccct
      781 gcatttggtt gagatattca aagtaaatcg ctttacgggt tttgggggga acgcctatga
      841 ctggctatac ccccatctgt ggtatgtgag ccaaataaga ttcgcgaagc tctttccggt
      901 ctatgtggtc gtagatgtcg atcgcctctt tcctcacatc gcccctcaat tcctggatga
      961 attcgcgagg catacccgcc cgtcggaggt gggtggtgaa ccagtggcgg caacagtgag
     1021 gcgagaaatg atcctccatc cggtcggagg tgggatcgtt taagcccaca aactccgccg
     1081 ccttggtgac ggcggtgtag acgccattgc ggtctagtcg attgccgacg ccattgatga
     1141 agagagcctt agacttgata ttactgcgcg atcctctgga tcgtagccac ctcttcagga
     1201 tgaaggcggt ctcgtcatcg aagaatatta tcctattcgt cctcttgggc gtcggcttga
     1261 gccttatgct ctgctccacc cagtcaatat cgtcgacgtc aagagtgatc agctctctcc
     1321 tcctgatccc ggtcttcgcg agaagggtga ttattgcctt gtctcggctg ttcatggtgg
     1381 agttgatgag gtgggtcatc tcttcgatgg agatgagctt gcgcatctgg ccttcgtcgt
     1441 tatctttgaa gcgccgtatg taccgctttc ttattgattg aaccgaattc ttatcgatat
     1501 acttctcata ctcaagaaac tcgtaaaagc ttgatagagc ggcaaagtaa ttctcgacgg
     1561 tcctctgctt tatcttgcga tcccttcgga ggtaatcgag aaatctcctg agaatatccc
     1621 tatcggacga gaataggtct agcttattat cctcaagata ctggacgaaa attcctattg
     1681 atgagaggta tcttcggatg gtttctcttg taagacctct cctctcgcag tcgtctttgt
     1741 actcttcgat caggtagttg atatctggga ggctctcact tgaaggtcga tgacctgtct
     1801 caaactccat atcggaggtt ctcatcattc tacccacctc cagccccgtg gagtggatgt
     1861 gattagaccg tatgcctcaa ggttatcaag ctcaacagat acggccttta ccagatccgc
     1921 gtcgcgagga tcgatgccaa gctcttcaag aatcttgtag ctatcgataa cgttgcctcg
     1981 cttcaggatg tttatgagtc ccttgctgta ctctctggtg cctttgaagt cgtcgtctaa
     2041 aaaggtagca ctccgatatc ttttgagttc attctcgtat ttatcgagaa caacgttctt
     2101 gagtctcagc tcatcctgga gctttcggat ctcgtccttc aaagatccca gctctttaac
     2161 catctccgcg cgaggcttga tatccgactc ctctgcgagg gcgttctcca ctatctcgat
     2221 cacgaacttt gaaagaggaa cgcccgcctc agatgcgagc ttatcccatc gcgccttatc
     2281 ttcggcggaa ggaagataga cgtagacata acgagacttg tcaggcttga ccaaggcatc
     2341 caccaaataa ataagtggac tcaatactat ttaaacatta ctggtaataa ggtgcgggga
     2401 acgggattcg aacccgcgaa ctcctgcgag acgggatctt aagtcccgcg cctttggcca
     2461 cttggcaacc cccgcccgga catcggtgca aaaccgctga agagatctcc ctttcgaggc
     2521 ggaccccacc gatggggcgg ggtttaagcg cggaagataa atagtttccc gcaagtgaaa
     2581 taccctcgat aaaggccgag gtgaggcgat gccccgacgc gatgcccccg atctcccgtc
     2641 gtccgtccgc cagtccggga agaaggggga gggaaaggac ggaaaagagg accgcaatcg
     2701 aggatatcag gacgagaaag gcgaagctga aaattttgaa gccgaagagt acgacttttt
     2761 gaccttcatc aaggcggccg gcttcgagga ggaggagatc ctcgcggtga tcggagagct
     2821 tgaagcctac aggtccatcc ccggcaccac cctacgaagg tacgtccacc gggtccaggc
     2881 ctccgtcgag accgacagca ggaccgcctt cctcaagggc gtgatcgtcg ggacggcgat
     2941 cagcgagcac ctggtggtcg atcgcggctc cgccgagggg aggatcgagg cggaggtgag
     3001 gaggagggtc tcggagatcc tcgcggagct ggagaggggc tgaggtcggc cgtccccgcg
     3061 agcgtcaccc ccgacggcca aacctgttat aggccaaggt acgatcctcc tccccatgga
     3121 gaaggggatc tacaccctcg tcctccgcct cccggccgaa aggctggtgg aggtcggagc
     3181 cctcgggacc gtccgcttcg aagccggcta ctacgcctac accggctctg cccggggccc
     3241 cggcggcctc gcccgggtcc gaagacacct cttggtcctg gcgggggata accccgcccg
     3301 ccggtggcac atcgaccacc tcctccccca tacggcgatg gtggcggtgg tggcgacgag
     3361 gaccgaggaa gacctggagt gcgccatcgc ccgggccatc ggcaaggacc ttacccccgt
     3421 cgaaaggttc ggatgcaccg actgccgctg ctccagccac ctccactact ccccggatcg
     3481 ggaggagatc ctggcggcgg taacgtcggc ccaccgtgga gctcgatcct ggtcagagga
     3541 ggaatcgcca ccttatcacc gtcgccgcga agaccagaag gccgaagccg gtgaggggca
     3601 gggtgtagcc gaggagggtc tcttttagat ccgaacccgt cgtatccttc aagagccaga
     3661 agtaggggtc gctgaggctg gagaggacga aggcccccgc cgagatcgag aataggatgg
     3721 tcaacggatc gagggggaga tccctcatca tctcggaggc gatcactacc gtcaccaccc
     3781 tggagccctg ggcgagctgg atcagggatg ccgtcaggaa ggggaggagg aggaggggga
     3841 atgcctcccc cagggcggcg tagagctcgg ggccgagccc cccctgggcg atgacggcgg
     3901 cgaaggcccc cgccccgcag aggtcgaaga ttatcacccc cgccctcctg gtggcagcct
     3961 tgatcatctc gccggccccc tccctcgcca gggcggccga gaggagggcg ccggcgagga
     4021 gggcgacctc cggcgacccc accagccagg cccagggggt gaggagcccc gccccgatga
     4081 gggccagggg gagggcgagg ggcgcccacg atctgagccg gggggagagg ggcccccctt
     4141 cggggatggc gccctcatcg ccggcgggaa gcctcctcat gtagaggaga cccgggagga
     4201 agaggaggag ggagaggggg aggccgaacc tcagcgcctc ggcgggagcc acccccaggg
     4261 accccgtcag ggccaccatc accggggatg gatagacgag gttgaagctg agaaccgaga
     4321 acgcccccgc ggtgaagagg agacgcctct ccgacccccc cgcctcccgc cccatccccc
     4381 tcaccaccgg ctccaggacg aggtacgagg tgatggagca catcgccggg agggccagaa
     4441 ggtaccccga gagccccgag gctaaaagcc ccctcccccg gccgaggagg cccaggaggt
     4501 cggcaacgac cctctccacc ccccgggacc ggcggagctg ctcggcgatc accgcccccg
     4561 agaatactat gattgcgagg gcggagaaga tccgggccag ccccccggag acggagccga
     4621 tcagctcggg acccatcccc gtcaggaccc cataggcgag ggccgagacg acgaggctga
     4681 ggaaggggga gaggccgaac ttcgccgtca ggagggccac cagaattatc gccaccagga
     4741 aggccataat cgggtccata gcccccatat aaccccgagg gactataaaa agatccccgc
     4801 ggagcttttc gcctagttaa ataatatgaa aaaagactta tactcaggtg gggattctta
     4861 agtatggccg taccgcctaa ttgttccgaa attcctttat agaaggaaaa caataaaacg
     4921 tttgcatagg gggagcttat gaagggtggc tcttggacgg attcccccca aagggatgga
     4981 gcagcgcatt tgttctgggt agctggtctc ttattcttcc tattatccat gcctgccgcc
     5041 tctgcctggt ccttcccctt ctccctcccc tttatatccg cccccgatgg gggctgtcag
     5101 atctccctgg acctggaaaa gaccgaggtg gagtacctaa gccccctcaa ctacaccatc
     5161 aggataaaaa attctggccg ggagcccctc tccgggatat cgatatccga caacttcggc
     5221 caggtggggt tcatcgcccg cctcccggag gggggggccc tcgacctcgc cagaaccacc
     5281 ccggctttga cctccagcgc cgagatccgg gtcgtcgcct cctctgacgg cgaggagctg
     5341 gggagggccg cggcccccgt tgaggtcgcc ccgcccccgg gggaggccgc cgccggagag
     5401 gcgcggtctc gacggacggc gaaggacgac ggggtgaccc tcaggacgat gtcctcctcg
     5461 ccggcgatgg agctggtcgt cggcgcctac cccccctccg tccggccggg ggagagggcg
     5521 acggtgagga tgaccgtcac caaccgcggc ccacaccccc tgaggggcgt cgagatctcg
     5581 gggccgggct ggaccgtcga ggtgggagac ctcaaccccg gtgagtcgaa ggtcttcacc
     5641 cgggaggtgg ccgtcgccga tgaggcgtcg atggagatcg tcgccgccgg gacgaacgac
     5701 ggaggtgagg ccgcctccgc ccgccagagc ctcaaagtat ccaccgtcag ccccgccctc
     5761 accgtcaccg tcgtctccat cccggactcc tccggcgggg cggggaggac cgagtaccga
     5821 ttaaagaacg ggggcgaaga gctcctgacc cggatcaccc tgaaggacgc caggggcgcc
     5881 accctgggaa tcctccccag gctggccccg ggggagacga agaacctgac ccagccgggg
     5941 agcaggtccg gcgaggggcc aatccccgtc gaggtgtcgg ccgtctcccc ggaggggaag
     6001 acggtcatcg gagaggtgca cgtccagtcc gccgccgtcc cgacgccgac ggcagccgcg
     6061 ggctcctcga ggagcggctc gaagccgacc gggaggatgg agtttggcac cctgccagaa
     6121 ctcgggacgg gcttaaagcc ggcgggcgat ctctccgtct tcggcggccc ggcggggggg
     6181 gcccccagca tcggctcagg gtttggagac ttcaacatca acttcgggat cttcgacggc
     6241 tccgtccctg cccccgccga cggaggagcc cggctcgatc cccgggggaa ggaggaggcg
     6301 gtggcggcgg atgaaaaggg cgccgaagcc tcccccgata tcgcggtgac cctccaggcg
     6361 aacaagaccc tcgtccaccg aggcgagagg atcgccttca ggtgtgccgc cgtcaaccgg
     6421 gggcaggtcc ccctcaccga cgtggagctc cgatgcggcg gcgaggtcgc ctccgccgag
     6481 ctcctcgccc cgggagacgg cctccccctg gagggatcga taatcgctga ggggcccctc
     6541 aacctgacgg ccgccgccgc cgccatcgcc cccgatggat cgagggcctc cggggaggct
     6601 acgctggcga taggggccgt atccccggcc ctccgcgtcg agatgagcca ggaacccggg
     6661 aggatatccc ggggggagag ggtctccatc gccgtccggc ttgagaacgg cggggacgac
     6721 ctccttacgg agatctccgc ctatgacgac ctcggggaga tcggacgggt cccgaccctc
     6781 aggccggggg aggtcgtcac cctcatgagg aactcgacgg tcgaggagga gctcgaggac
     6841 gaggtgaggg tcgtggccat cgactctacg gggagggagg tgcggtggtc gcaggtcctg
     6901 agcctgaagc tctccgagcc cggcctcagc ctcgaggtcg agccgaaaga cgccgccgca
     6961 taccccggcg aggcggtgga ggtggtctgg acgatcagga acaccgggga ggtcgacctg
     7021 gtggacgtga cgatggtgat cgatggcaat agcagcttcc ggcttcctgc catcgccgcc
     7081 ggggggtcca cccaggtatc ctcatcccac cagcccgccg ggagccgggg ggtcctggcc
     7141 cgggctgagg gaaggaccgc cggcggggag acgatctcct ccgaggcgtc cttcctgatg
     7201 agggtggtat cgccggggtt gagcctcaac gtcaagccct ccgaggtgga ggcatctctg
     7261 aagaagccct tcaacctgac gttcctggtg accaacaccg gcgacgaccc cctccaggag
     7321 gtgacgatct cagagaggag cctggggacc ctggagagga tcggacggct ggagcccggg
     7381 gacttcaggg tggcttctca cgacttcttc accgatacca acaccacctt caggctggag
     7441 gcgacgggga tcgactcccg ggggaacggc gtcaacgaga gccgggaggt ggcggtgaag
     7501 ctagtcagcg ccaatatcga gctatccatc cggccggagc cggcggaggc cgcccagggg
     7561 ggctccgtcc acgtcacctt ctccgtcgag aaccggggcg aggtccccat cttcagcacc
     7621 ttcatcatgg ggaggaccct ggggcacctg gggacgatcg actacatctc cccggggtcg
     7681 ttgcggagcc tggagaggga tctcgaggtc accggggaga tggaggaaga gatcaccgcc
     7741 gagggtttca cccgggacgg ctcctcggtg agggacagcg agctcctctc agtggggctg
     7801 aaggtggaga ccccgatcaa agaggccacc cccgaggcct ccggccccgg gcccgggagg
     7861 gggacgaccg ccaggatcca agaggcagcc ccgggggagg aggtcttgcc cgatgatggg
     7921 aactacacct tccctccggc ccccgccgga aaggaggagg gcgccgggat agccggcctc
     7981 ctggagaggc tgaaggggat cctggagaag atcaggctga tgaaggacga ccccgtgggg
     8041 atggagggct acgatcccgg ggccctcccc gccggatcgg gggagggggc ctcctcggcg
     8101 gccccccgct ccgggagcct cccgggagcc cccaccacct ggtctggata cggaacccac
     8161 caggtgggag ctgacccgga gccccagacc gcccgcccag ggggggcggg atacgcctac
     8221 tccggcctgg gaggggcggc gggggacctc tcgcggctgg ggcccaggcc cgagacctca
     8281 ggatatgcct cctcggcgga ggggccggcc tcgaagtacc tgacgggccc ggggaccgcc
     8341 ccggaggcga cggtggggac gtactcggcg agcggccccg caccatcgta cctctcctcg
     8401 ccctggacgt cggccgggga tccaggatac accccctcaa agagcggccc cctctcgccc
     8461 atcaccgccg ccgggggctc gccgacgatc gcgggctccg gggcccccat ccgatatgag
     8521 agctccgacc tcaggtccgc ctccgaggcc tccccggcgg tcggcgactc gaggggctcc
     8581 gcctacgatt cgagccgcct cgtccggtcc gagcccaccc cgggctacgc cggggcatcg
     8641 gctccgaagg aacgcctcag ccaagagacc cccatccagt cgaaggcccc ttccagggag
     8701 atcaagctgg tgatcggcga cacctccagc ctccaggtcg accgcccgcc gaggatcatc
     8761 gacgtggggg cgttcccgcc ggagccagcg gcctggaccc ccgtggtcct ggcggtccac
     8821 gcctccgacg acatcgggat ccagtccgtg aacctcctct gggccacccc caccaccgcc
     8881 atcaccaggc tggatatggt ggacgcgacc aagatcaaca cccagaggat gaccctcgag
     8941 gagggggacc tcaaagaggg gtactggagc tatgagatcc ccggccaggc cgccgggacc
     9001 tacatggccg ccttcgtcag ggtcagcgat ggggagcggt gggcggagga cggcccctac
     9061 atcatcttct ggtccgaggc cccccccaag accgaggcgg cgccccagcc cagcgccgag
     9121 ccggaggttg ccaagggcga ggcggccgtc gggacgaaga aggaggggat gctcttcgtc
     9181 gagtcgacga cggtggtggg gaggggagac gtctcgatca agaacgagtt ccgggaggac
     9241 agtgcccgtt accgcgaaga gctggacggg agaggatcga tcgagatgca gtcggagaag
     9301 atgataaaca aaggaaaccc cgtcgtcaac atcaccgatt cgagacttct cgtcttcgac
     9361 caggggtacc tgaaggggtt caaggtgatg cagtctccgg gcttccacgg cggcatgggg
     9421 gccagcgtca ccgagcggtt caacgccacc accctagaga agagcgaaac ggggacgata
     9481 tcctccctca acaggtctca acacgccctc ctcttcaaca gccagcaggc cttcgagggg
     9541 atttggggaa cccgaacgga gtactcgaac ttcaacaaga agataaaggc ggaccagacc
     9601 ctccacggaa ccttcgagac ccagaagagg atcaccttca aggactgaac ggcccccagg
     9661 aaaagagagg gtccgatgac cccgccatct ccagaggacc cgggatctga aataatgagg
     9721 ttctcgtccc ggaggtcttt tagggctggg gccttccggc cctgaccgcc cccttccaga
     9781 tctcgtcggc cctgtccacc acggcgacga tctggtcgtg aatctcgagc cggtgcttga
     9841 tctcccccgc cttgagggca gagtccaccg agagcctcat catctcccgg ctctcgcccc
     9901 tgagatcctc cgactcctgc cacatctcct cggcagcctc cgggtctgcc gaccggaggt
     9961 ccaccgaccg gcttcggagc tcgtcagacc gcctcagaag ctctgcagcc tccttcttca
    10021 gccgttccga ctccgtctgg tgggacaaca tatcctcctc catctcctcg aagctcttct
    10081 ctacaaagac ctcatcgacg ccgaacatgg atctatacct cctcgatcat cctcagaaag
    10141 agctggtgga acctatcgtc ctccgtcagc tcggggtgga aggcgaggcc caggaggttc
    10201 tcctccctcg ccgccaccgc cacctcctca atcctggcga ggggctcgac cccttcgccc
    10261 cagccgacga tcgccggggc ccggatgaag acggcgcggt agggggagtc gaagcccgcc
    10321 acctcgaggt ccgcctcgaa ggactccctc tgggacccga aggcgttcct tgccaccctc
    10381 acgtccagaa gccccagggg aagaaaccta gtctcctcga cgatctccgt cgatacgact
    10441 atcagccccg cgcaggtggc catgatgggg acgccggacc gggaggctgc ggcgacctcg
    10501 tctccgaccc ccgtccgcgc gaggtgccgg gcgatggtgg tgctctcccc tccagggagg
    10561 acgatcccgt cgcagtcggg gactatgccc cctttgcgga cctccaccac cgaacccctg
    10621 gcccccaggg ccctctccat ggcaccgacg tgctcggaga cgtccccctg gaaggcgacg
    10681 acgccgatcc tcgatctacc aacccctggt ctggaggatc tccccctcag agagggtgcc
    10741 ggcatctatc cccttcatgg ggtcgccgag gcccgtcgag atctcgacga gcttttcggg
    10801 gttgtcgtag ttgttcaccg cctcgacgat cgccgaggcc atcagttcgg ggttctggga
    10861 cttgaatatc ccggagccga cgaagacccc gtccgccccc atctgcatca tcagggaggc
    10921 gtcggcgggg gtggcgatcc ccccggcggc gaagttgacc accggaagcc gctgcatatc
    10981 cctgcactcg aggaccagcc ccagctccgc ctcgatctcc ctcgcgagct tcgctagctc
    11041 ctcgtcggcc atccccttga ggctcctgat cgagcccccg atcagcctca tgtgcctgac
    11101 cgcctcccgg acgtccccgg tcccggcctc ccccttggtc cggatcatgg cggccccctc
    11161 cttgatcctc ctcagggcct cccccaggtt cctcgctccg cagacgaagg ggacggtgaa
    11221 cctggactta tcgacgtgga actcgtcggc tggggtcaaa acctccgact cgtcgatcat
    11281 gtcgaccccg atcgcctcca ggatctgagc ctcggcgaag tggccgatcc tcaccttggc
    11341 catcaccggt atcgtcacct cttcgatgat ctccatgatg atcttggggt ccgccatcct
    11401 ggctaccccc cccatcttcc ttatgtcggc ggggaccgcc tggagggcca tcaccgctac
    11461 agcccccgcc tcctccgcga tcacggcctg ctcgggggtg gtgacgtcca tgatgacccc
    11521 ccccttctgc atctttgcaa atccgcgctt gagcagctcc gtgccgaaac gtagttcttc
    11581 cagttgcatg atagtcagat gccccgatct tttttaagcc gcttacaagt gagggccgaa
    11641 taaataaact ttgctcccag accttctgcc gccttcgaca aaacggtatc ggatggccga
    11701 tggaggggcc gccgccgcgg cggaagggcg gtgcccccat ctccggccta ttcgcggtag
    11761 atctcgtaca tgtagatgtc cttatccccc ttccggtcgt cgacccagac tatcctgtca
    11821 ctcccggtcc ggggatcggt ctggttcccg ggaccggtga agatctccat ctccctcccg
    11881 gtggccaagt ccagggcccg gatatcccag tcgccgttcc gatcgtcctg ccagactaaa
    11941 accccgtccc ggaagtgggg gctcgtctgg ttagacgggt cggaggtgag ctgctcgacc
    12001 ttgccggtgg ccagatcgtg agagtagagg tcccagttcc cgttccggtt gtcctgccag
    12061 accaccaggg acccgtcggt ggtgggatgg gtctggtccc cgggaccggt gtagaccggc
    12121 tttcgggtag tcccggacca gatgtagatg tcccagttcc cgttctggtt gtcctggtag
    12181 accacctggc tcccgccgac ggaggggttc tctcccctgg gcgggatcgc ctccatcgcc
    12241 tcggtggtgt agaggggctt cttctggatc cgccatccga aggccgctat ggggaggtca
    12301 ccccaggcca ggatctcctc gctgatggag ggcctcgaag ggacggttat gcccctctgg
    12361 agctgggtcc tgttgacggt gggtatgtcc agggccctga tcgcccagga gttgaactcc
    12421 tcctcgaggt acatccagac gacgacgtcg ttaattatgt cggggtacat gtcgaactcc
    12481 ctccccgccg ccaggggcca ctcgacgttc tcgacgaggt tgtagaggta gatgtcgggg
    12541 ttctgagttc tgttgtccat ccatacgacc cagtcgccgc tgacggcggg gctcgtctgg
    12601 tcccccgagg cctgacagat gacgctcgtc tggtcggagc cgaggtttct gtacccgaac
    12661 cttccgctag gcgagatgag ccaggcgagg cgctgatcga ggtcgaactc cacctccccg
    12721 gggctccggg ccacctcctt gtccttccag gtgttggggt cgaagctgtg gatcgaccag
    12781 cccccttcga cgggctcctg gtagactatc ctcttttcgt agaccttggg gctcgtctga
    12841 actccgggcc tggagtaggc gcgcccccac ttcctatccc ataggtagta gctgatgtcg
    12901 ccggtcgtcg agtcctccca gacgatgatg ttcccgctga cgtgggggtt gatctggtcc
    12961 ccctctccgg tgagcttcgt ctcctcgcca gtggcgaggt cgcagacgta gatgtcccac
    13021 tcgccgttcc ggtcatcctg ccagacgagg aggtcgccac tgatcgaggg gttgatctga
    13081 tctttgggcc tcgaagcggg gggctttatg gccgccccgg ccaggccctt cgccgagacg
    13141 tcccagctcc cggtggagtt gtccatccag gcgatcctcc tcccgctgac ggaggggctg
    13201 acctgggcgc cccggccgtc ggcgacgacg atctcctccc ccgaggtgag gttcagggcg
    13261 aggatatccc agtcgccgtt ccggtcgtcc tgccagacca ggaggtcgcc ggaggcggcg
    13321 gggctcctct ggtggctggg tgcccggacc aggggccgct cgaaccggga gtagaagtcg
    13381 aagccgaaga ggtcccagtc cccgccccgg ttgtcggccc agacgatgag gctgccgttg
    13441 gtggcgatat ccgccttctc cagaaggagg atcgggtcat cagccaccga tctcgtcatg
    13501 atctggggcg tcccccggct gtagtccatc cataccagcc ggtcgccgct caaaaactga
    13561 gatgctccgg cgaggttcac caagaaggga aagcccaccc cgtcgttagg cctctggtgc
    13621 aacggccccg tgacgacggg gatctccttc atcagatcga ggtcgtagag gtatatgtcg
    13681 tagtccccgt tccggtcgtc cagccaggcg atgagcctat caaagatgac gggctggacc
    13741 tgattgttga tgtcggtggt gatcaccatc tcctcgtcgg tgaggaggtt cttcccccgg
    13801 atgtcccagt tctcattcct gtcgtccatc cagacgatca gatccccatg gagccaggga
    13861 gcttgctgct ttccggggtc ggtcgtcacc tgaacctccg agcgggtgga gaggttgtag
    13921 acgaagatgt cccagttccc cgcccggttg tccatccagg ccacccgatc cccgtggatc
    13981 atcggctgcg ccttcttcga ggcgccggtc gttatctgga tctcggatcc gttggagagg
    14041 tcgtggaccc ggatctggtc gtcgccggtc cggtcgtcga tccagactac ccggccgtcg
    14101 gcgaccatac cataccactg atctgagggg tcgtcggtca ccctcgtctc ctcccccgtc
    14161 cggaggtcgt agacgaagag gtcgaggttc cccgatcggt tgtcggtgta gaggattatg
    14221 tcgccgctga tggaggggct gctcttctcg tcctcggagt cggtgatctg aatctcctgg
    14281 gaggtggata ggtcgatcat gtagacgttg aacttcccag tccgatcgtc ggtccagacc
    14341 acccggtccc cgtcgatcca ggggtttgag ctgttgtgag ggccggaagc tatctcttcg
    14401 tcggccgtca gggtgaagtc gaatatgtaa acgtccgagt cgggggcccg gtggtcggac
    14461 cagaccaccc cctgcacccc cagggcggga ttcatcttga cggacccggg gctgacgagg
    14521 gcctcctcgc tggtatccac attgtagagg taaacctggg gaactccggc cctgtggtcc
    14581 tgccacgcca cgaggttccc cttcaccgag ggggcggtct ggagccccgg cctctcgatc
    14641 accgtctcct ggccggtggg gaggtcgagg atgactatat cgtagtctcc ggtcctgttg
    14701 tcggaccaga ccaccagatc gccgtctatg tccgggaata cctgctcccc cggacccgtg
    14761 gagagggggc tctcccgccc ggcctcgaga tcgtagatgt agatgtcgga gttcccgttc
    14821 cggtcgtcgg tccagacgat caggtcgccg ctgatcttcg gctggatctg gtctttggga
    14881 tcggtggtga tcctccgctc ttcgccggtc gcgagatcgt gcatgtagac gttccagttg
    14941 ccgtcccggt cgtccatcca gaccacccga tccccgtcga tgtccgggag gaactggttc
    15001 ccgggggctg tggtgatcgg ggtctccgcc cgggtgacgt aatcgtacat gtatatgtcc
    15061 gcctccccgg tcctcatgtc ctgccagacg atccgatggc ctgagaccga gggatggtcc
    15121 tggtggccgg gatagaagca gacgcccctc tcggtccccg tctccagatc gtacatgaat
    15181 atatcgaagt tgccgcttct gttgtccgcc caggcgacga tatggccgtc tatggcgggc
    15241 tgctcctgca ggccgcccca cgtgctgatg ggacgctctc ttcccccact caatccggat
    15301 gcgacgcctg ccataagcaa cgccgccaac aataaaatga tgatccgcct cgacatctta
    15361 gcactcatcc tgtcgaagtt cagctgacga tctcaactcc gagaaggtgg ggagggcgga
    15421 tatataagga cttacctctc cgccgggatt gctggcctct ttaggaatat aaacctcaat
    15481 ttagtggaca atgagggtaa atgcggcggg gtggcagggg ggctacttcc cccggacgac
    15541 tatggtgagg acgtcctcgt cggcgaggac gtggtcgagg cccaccgact ggcccgggaa
    15601 cttggcgctc ctcccccaga ccttggcgta ccggaagagg cggacgaagt cccggtggag
    15661 gtgctcgcag acggtcctca ccgtcgaccc gtccaggatc accagcggat ccttcatgtc
    15721 cgcctttccc cgctggggct tcaggtatat cctgatgaac ccgagggtgt cgtatatcag
    15781 ctccttcagc tcctccaccc cgaccccctt ctccacggag aggggtatga actctctgcc
    15841 tatcgtactc ttgatatcct cccggacgtc gtcgtactcg aggtcgatct tgttgatgac
    15901 ggcgagctga gggacgtata tcctgtttcc ggcaaggaag tcgacgaggg tctcagtatc
    15961 cacgtcctct cggatggtga tgatcccgtt gacgatcccg taggcccgga ggatctcggc
    16021 gatctcgacc tcggtcatct gggtgagggg gatggtgttt cggacgatga tccccccctt
    16081 atctgagggg ctgagggtga tgtcaggggg gaccttatcg agcctgatcc ccgcctcgta
    16141 gagctcccgc tccaggacgt cgaggcgata gttgtagacg tcccccatca ggaggattat
    16201 gtccgctgcc ctcgccgcgg tgatcacctc ccggcccctc cccttcccct tggatgcacc
    16261 ctttatgatc ccgggcatat ccaggatctg gatcttggcg cccttgtact ccatcacccc
    16321 ggggatcacc tccagggtcg tgaaatggta tgctcccacc tcggcgtaag accccgtcag
    16381 aaggttgagg aggctcgact tgccgacgga ggggtagccg acgagggcga cggtggcgtc
    16441 ccccgacttc tttacgtagt agcccttacc gccaccggca cctttgatcc tctggagctc
    16501 ctgctgcgcc ttcagcttcg ctatcttggc cttcagcttt ccgatgtggt gctctgtggc
    16561 cttgttcttc tgggtgctga agatctcctc ttctatcgcc tggatctgct cgtcgatcgt
    16621 agccatcggt aacctgccta taaagggccc cggaccgcct tttccgccat tcaccgccgg
    16681 gatgacgcat ctctcagccc cctggagccc ctcagatatt aatggtttgg tcaacccgca
    16741 ggggttcggc ggcagaagga tgggagccct cgcccgggct atgggaccgg ggctgggacg
    16801 ccgggcagca gacgaccaca tatcggcggt ggccgccgag ggccgcccgt cggggtcggg
    16861 cggaccccgc gggaggagga gccaaaggta taaatatcgc ccccccatga ctatccccga
    16921 tccggggtat agtatgaagt ggcaaggaag ttcaaagagg aagttcaccg gcggaaggct
    16981 gatctcaaac cggggaaagc agaagcatga gctggggaga gaggctggag agccgaacgt
    17041 cgacaccacc agaaagaagc agatcaggac ccgaggcggc aatatcaaga tgaggctcct
    17101 ccgatgcgac gtggccagcg tcgcggatcc cgtcaccggg aggtccaaga ttgcgaagat
    17161 cgaaggggtt aaggacaaca gcgcgaacct caactacata agaaggaaca taatctccaa
    17221 gggcgccatc atcaagaccg atctcggaga tgcaagagtc accagcaggc ccggccagga
    17281 tggggtggtc aacgccatcc tagtcgccaa gtgatgccaa taacgaagca attttgatgt
    17341 acctgatctt caggtgttcc aggtgcgggc gccacctcta cgccgaggag gggtccgcca
    17401 ccaggacctg cccctgcggg aagaggatca agctggtcca ggtgagggtc ctctcccggg
    17461 ccagggatga acgagaggca ggggacctgg tgagggccct ccaggtggag gggcgcggga
    17521 tgacgggctt ttctccggcg ggatgatcta gccgaggctt ctcatccagc tcttgcggcc
    17581 gggggggggg agccgaaggt cctctttgcg tgacgggcat cccacgggtc cagagcgggg
    17641 acccttcaat aagtatataa acctcgggag atacgcccgg actggggtga tcaattgtcg
    17701 gaagggcatt taaaagcgtc attggtggtg gctgcagcct tggtcctggc cttatcggga
    17761 ccgctcgcct cggccaccct ctggagttcg gggatttacg gccttggata cggatccttc
    17821 tcttcggctt cgatgagcgt caggtcggag gacggggtgg ttaagctcgg aagctacggc
    17881 atcccgacga ccccccaggt ctcaaaggtg ccggcggtgc agcttggaag ctacggcatc
    17941 caggacgtca aggcgccgga gaccccgggg gccctccagc tcagcggcta cttccagaac
    18001 agaggaagct atacgaacat gtacttcacg ccgatcctcg gcggcacctt cggcggtagc
    18061 ggcggctgca gcagctgcgg ctgatctatc gagaagccca ggggtgggga accacccaat
    18121 tttttaacca tttgggatcc aggaccgggg cctccggccg aagggtccgg agttctccaa
    18181 gtccatgccg cctcagatcc accggtagtg gaggaggacc tcggggacga agtcgtagag
    18241 gaggctattc tcgacgtaac cgaagaagag gcagcctccg cactcccgga cgatctcctc
    18301 tctcctcctc ttcgaggcgg cccagacgga agcgagcccc tgggatgcgt tccccagggg
    18361 ctccttctgg accctgcagg catggaccgt cccgtcggcg gcgacagtga ggacgatccc
    18421 cggagcgtgg caccgaaaat cccgcctctg gtcccggacc atcttgaggt aggtgatgga
    18481 gttgatgatg gggccgcccg tcctcttgat gtcgatcagg ccgtcgatgg accgcctgta
    18541 ctcggaaagg tcggcttcgc cgatggtgta atcgtcccag acctcgccca caactccggg
    18601 ggcccggtgg accggctcaa aggagatgag gacgccgagc tcctccgcca ggcggacgag
    18661 gtccgccagc tccccaaggt tctttctgct gatgacgcag ttgatcagga cccggtggcc
    18721 ggccctccgg gccgccctga tcccctcgag gaccggctcc aggggagccc cccggatctc
    18781 cgggtaggag ctgatcccgt cgacggagac ggatagaagg tcgaggcatg ataggtcgcc
    18841 gacccgctcg gataagaggg ccccgttggt gatgagggag gtgaccatcc ccagggcctt
    18901 agcgtggtgg aggatggcgg ggagctccgg ccggaggagg ggctcggcgg tccaggcgtt
    18961 gtagacccct atcccgaagg atcgagcgtc gtccagcatc gcgaatattt cgtccgaggt
    19021 catctcccgg ccgggggtcc gccagtactc gcagaagggg cagcggaggt tgcaccgggc
    19081 gttgatcccg tgggatagga caaagggcct cctcctcacc ctcatctgga cgagggcctg
    19141 gagggcgacc ttggggttga aggaggccat cttcgccttc tccccctcac ttcaggagcc
    19201 gggccgggac gacctgcccc gagaggagga cgctctggtc ctcgccccgg cggatcagct
    19261 ccgcccggcc gccgcccacc aggacctcgg ggcaccgggg ctggccgttg tagaccgagg
    19321 ccatggagaa gccgtaggct ccggcgtcga gcatcgcgat gaggtctcca cgctcgatcc
    19381 gggggaggtt ccgatccttt gccaggacat ccccgctctc gcagatgggg ccgacgacgg
    19441 tatacctctc ctccgccctc ccatcggcct tgtttgcgac gacaacgtgg tggtaggcgt
    19501 cgtagagcat cggccgagcc aggaggttga agccggcgtc gacgccgacg aagttgacgt
    19561 gggccctctt gacgacgttc accctggtga ggaatatggt ggaatcggcg acgatgcttc
    19621 gccccggctc caggatcatc ctcgggctta tcccgagcct ccggcacccc tcctcgaaga
    19681 ccggcttgat gccggatgcc agctcccacg gcgtcggggc cgacgtctcg ggggtgtatg
    19741 gtatgccgag gccgccgccg aagtccatca tctcgatttt gcccccgacc tcgacgatcc
    19801 ggcccgccag ctccatcatc ctcgccgccg cctcgacgaa cggggcggta tcgaggatct
    19861 gggagccgat gtggcagtgg aggccgacgg ggttgacccc ctccaggccc attgccgtct
    19921 cgtagacctc gacgagatcc tgccagggga tcccgaactt ggaggtttta aggccggtgg
    19981 cgatcttggg gtgggtcttt gcggagacgt cggggttgac ccggaaggcc acctccgcct
    20041 cctcgcccat ctccttcgcg acctcggcca gggccagaag ctcatctagc gagtcggcgg
    20101 agatccggac cccggcctcc agggccatct ttagctcctc ggccctcttg gagttgccgt
    20161 tgaagaggat cttctccatc ggcatccccg ccatcctcac aagagacagc tccccggcgg
    20221 agaagacgtc cgcccccatc ccctcctccg ccagaatccg gaggagggcc aggttcccgt
    20281 tcgccttgac ggcgaagtag atcagagctt cagggaaggc ctcccggtag gcccgggcgt
    20341 tcgcccgcag ccgcgcctcg ctcgtcacgt aaaggggcgt tccgaactcc tcggcgaggt
    20401 cgacgacgtc gaccccgtcg atggtgaggt gccccccttc cgccctcagg tgatccttcg
    20461 tcccgaacat acctctggat gaaggggtcg gaggcgtaaa atctttttcc tgcctttcca
    20521 gtgcgaagat ctcaccccgg ctcttcggag ggcgaaggag aggggatcat cgccacctcc
    20581 acccccgcca gctctaaaaa ccggagcgcc tcgtcgtcgg ggtaggggtt gccgtagacg
    20641 accctctgga tctgagagtt gatgatgatc ttggcgcaga ggatgc
//
LOCUS       DASM01000059            3257 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_184401, whole genome shotgun sequence.
ACCESSION   DASM01000059 DASM01000000
VERSION     DASM01000059.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_184401"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    2086..2095
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          580 a          999 c         1026 g          640 t
ORIGIN      
        1 gcgacggtga gggcgagaga cggccttctc accttgagcg ccctgggcgc aaagctcctc
       61 tacgactttc ttcctccccc ccggcagagg gtcgttctca gaagggatgt ggccccctac
      121 gtggcgaagg gggggaccgc cttcgcaaag cacgtcgtca gcgtcgaccc cgaggtgagg
      181 gccggagagg aggtcctggt cgtagacgag gacggaaggc tcatcgccac cgggagggcg
      241 gtcctcgcgc cggaggagat gatggagatc cgccggggcc ctgccgtcct cgtgaggtcc
      301 ggccaggagg aggactgagg ggcccttacc tcccccccac ggccggagat catccattat
      361 ttttttatcc tcggaagatg ggaatcaagc ttttctgaag ggggacatcg cccggaggct
      421 ctcggccacg gagacgatcg cctctccgac gcggtcgacc tcctcgtcgg tgttctcccg
      481 ccccagggtg atccgcaggg acccgtgggc ctcctggggg cgcaggccga tggcggtgag
      541 gacgtggctt gccaccgtct tcttcgagga gcaggcggag ccggtggaga cggcgatgcc
      601 tttcgcgtct agccgcagca agagcgcctc cccctcgatg taagagaagc cgaggttgag
      661 gttatttggg agcctcttcg tgcgagagcc gttgacccag gtgtcttcga cccgctccag
      721 gacgtagtcg gcgagcctgt ccctcatccc gctgatcctc gccatctcct cctccaggtt
      781 cctcccggcc agctccgcgg ccgcggcgag gccgacgatc ccggggacgt tctcggtccc
      841 ggacctcatc ccccgctcgt ggccgccgcc aaagacgatc ggcttgattg cggttccctt
      901 ccggatgtag agggcaccga cccccttggg gccgtggagc ttgtgggacg agatcgagag
      961 gaggtccacc cccaagtcgt cgaccttcgc cgggatcttg ccgacggtct ggacggcgtc
     1021 ggtgtggaag acgatccccc tcgaccgggc gacctcccca gcctcggcga tgggctggat
     1081 cgtaccgatc tcgttatttg cgtgcatgat ggagatgagg atcgtatcct cccggatcgc
     1141 cgcctccagg gcctccacct ccaccagacc ctcccgggtc accgggagat aagtcacctc
     1201 gaacccctcc tcctcctcca gcttcctgca gggctcgagg actgcggggt gctcgatgga
     1261 ggtggtgatg atgtgtcttc ctcggtcgcg gttcgccagg gcggcgcccc ttatggccag
     1321 gttgtccgac tcggtcccgc cgctggtgaa gtagacctcc tccgccttcg ccccgatgag
     1381 tccggccacc cgctcccggg cctcctccag ggcggcccgg gcagctagcc ccggctggtg
     1441 gaggctggag gcgttcccga agatctcgac gaaggccttc gtcatcgcct cgatcacctc
     1501 cggggccatc ggggtggtgg cggcgtggtc catgtagatc ctcttcatgg gaaaatccga
     1561 ggagcccata gggtaaatag gcggcgaggg ttaggggagg agacggtcga ccggtattgg
     1621 ggctagttgg acatagggat cgtggtctct cgacagagcg gagggctttc atgggaaggg
     1681 gaggccggtc gttcggggat ccgggaccgt gaggtgaggg atatggcggc ttcaaaaggg
     1741 cgtcgacgag aacttataag taagcagccg gccgatcttc ggccatgtta gcctacaacc
     1801 cgctgatcat ggtggggatc gtcctcatca ccgtcgggat cctctacacc ctggtatcgg
     1861 cccgtggggg ggtcgtcctg gcagggctgg ggacggtggc ggcgggggtc ttcctcttca
     1921 ctatcgtccc aaaccccctc aatcccatcg ccttcttctt caacggcctt ctcgtcctat
     1981 gcggcctctg gatggtggcc gtcggcgcta gaggcgacgg cgtggagcga taacctttat
     2041 atgggtggta tgcgtccacc aatccgcagc ggggccatag ggtagnnnnn nnnnnggcgt
     2101 tcaaatcgcc gtggccccac atttcgcgtt ttccgtccag cgttccaggg cttcttcatc
     2161 ttcattggta acgctgccag aatatctttt cgattcgacc gatctttagc tattccaggg
     2221 agtctgacgg atcttccctc tgccgaaggc ccgcccagaa tggccgaggc caagactcac
     2281 ggttctctca ccctttcata ttcttgtcac cccttcccta ataccctatt tcatatcaat
     2341 ttaaccaata gtgataaata gagcctattt attctatctt cgtaggacta aatcataaat
     2401 atggtggtat aaaaatgaga gaaatatcca aaagaatgag aaaaatcgca aaatcgccgt
     2461 ttctcctttt attggggatg gcgatcttct ccgcgatggt gccggccttc ggctacgagg
     2521 tggtggtccc gggggacgag gacggcgatc tggtgatctc agacggggag ctggaggcgg
     2581 cccaggccgc cctcgccgcc ggcgagatca ccgacgaaga gctggccaag atcgagcata
     2641 tccacgagaa ctnnggccgg gaggtcgtct tctacaagcc cgtcgagcgg atcatcaccc
     2701 gggagcccga cacctgccgg atagtcatag ccctgggaga aggcgagaag gtcgtcgcct
     2761 cggagcaggc ggtgaagtcc tgcctctgcc ccaccacctt cgagaccttc gaagaggggt
     2821 gccttgaatg ctacagttcg atcctcggcg gaaggctcgc agacctcccc gagacgagca
     2881 cccgctacga catctactac gagctgatgg cctccctcaa ccccgacgtc atcatcacct
     2941 cgggcgccac caacgttccg gacttcgaga gcaaaatcgg ctgtcccgtg gtggtcgccg
     3001 gaggggacgg ctggaactac acccgggatg acggcctcta cggcatgatc gaggtgatag
     3061 gcgacgtcct ggagagggag gacgaggcgg aggagctgat cggcttcgtc gaggcgaaga
     3121 tcgagatgat aagatctgtc tccgacggca tccccgagga tgagaagccg ttggtatact
     3181 tcgccccccg gggtgctaag ctcggcttct acgaccccaa ggagggccgg gacttcacca
     3241 ggaccttcac atcctat
//
LOCUS       DASM01000060            3323 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_89334, whole genome shotgun sequence.
ACCESSION   DASM01000060 DASM01000000
VERSION     DASM01000060.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_89334"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          514 a         1137 c         1011 g          661 t
ORIGIN      
        1 aaggtgtatc tatctagcta tttcagtcgg tgggcatcaa catcatgatt ttgagcgtct
       61 tcttgatgat ggaacttctg cgtatcggat tcacattcat ctccgggttg ggcgggagga
      121 gctattctcg cattttaata catatcccag agcccgaaga caggtcgatg atcgtccggc
      181 tcgcaaagct ggtttgcgag catcttcact gctcagaaat aggacgaagc tagtttaata
      241 actttgtggt ggaggttaag atcactcccg gtgtcccctc ccttcctggg gcgggaggat
      301 gaggatctcg cccccaaacc tcaaaaactc gacctcctcc cccactccgg ccccgaggtt
      361 ggcgggcctg gatgcggaga cgatcctgaa gctgacgggg tccatgacct ccagaacgtc
      421 ccggtcctcc gccacaacca cacctctctc ggcggaggag cggttcccca ggacgggggc
      481 gtcttcagcc tcttcttctg agagatacac cgtcgcgccg tcccggagag atctgcaggc
      541 cgtcctcttc ccctcaaacc ccctcacctc caaaaagcgg cctcgacccc tgatcaggtc
      601 gccagccttg agccgcggga gcctgacgag gatgctcgtc cggtagacgt tccttccgtc
      661 cctagtcccc gcaagcttga aggactccag ggtgctcccc ccgaaccggt catggatcgc
      721 cctcgcgatc gccctcccct gctgggtcga ccccaccacc aggtcgatcc cgcccttgac
      781 ggccttcgtc tcctggatga aggagaggcg gtctcccctc tcaaagcagc ttttcgtgac
      841 gtcctcggcg atcctccggc cctcatccag ctccgccggc cccgggggtc ccgccgtccc
      901 cctcaactgg accgtcgcct cgtagtagtt tcccgccatc cggctgcacc tctcgcaggt
      961 ggccctctcg gcggtggccc tgacagtcct ctcctccgag gcggcaaggc cgccgaagtt
     1021 cccggagact cggacggtgg cgaggtactg ggtggggttc aacttcgaga tcgagatatc
     1081 tacctgtggg tcttcgaggt cccggtggag ggcgatccct ccggccacgg cctcgaagac
     1141 catctcctcg acggtgcccc cagtatcccg ccagcgcccc tggacccgct ggaagccgca
     1201 gacgggacac gccgtcacct cgacgtggtc agaaacctcg aggagggtcg cctcctccag
     1261 tcggcatcgg ctgcagaggc ccagggcgga tggcccgccg caccgggggc agacgctctc
     1321 tagcctcaca tccgcaccat ctgggcagtg ggaacgccgt cgatcttgag gatgtttctg
     1381 ccgtcgatgc agttcgtctt gatcccgcag gctaccgccc tctcccccac catgttggcg
     1441 atcgacgccc cggccagggc ctccgccacc tcccggaccg tcgccacctc ctccccgaag
     1501 aaggccgcct ccaccgtcag ggagagggcc ccctcatcga aggtctttcc gaggagctcg
     1561 ctgtcgcaga ccgccaccat cacctcgcca cgaaccttgt gaaccttgag gtacatcgta
     1621 tctctcatct cgtccatgtt atcccatcgt ctcttctcga tagcatcttc aggccttcag
     1681 gggagcttca agaagccggc cctcggctcg aagatctcgc cgttcgtccg gagcctcttt
     1741 atgatctcct cggccttatg cctctctatc ccgagcttcg tctccgccct ctccaggacc
     1801 gcttctacgg gggcggggcc ctggtgctcc gaggcgatct cccggacgag gtcgatcatc
     1861 agcctcgtct tgtcccgggt gctcttgctg atccccaccg ccaggacgtc ggcgtcgagg
     1921 aggccggtct cggggtcgat ccccacctgc tggaggcagg agtcgacgat tcggatgacc
     1981 cgttcggcgt cgccaccggt gatctcgtcg ctcagccgga gcctcgcgct cgactcggag
     2041 aggcgaaaga gcgcctccag ctgcctcgcc gtcaccggga cgggcttgtt tgaatcctgc
     2101 ccctgctttc ggaggctgac gtagtacccc tccagcctac ccctcgcctc gggggtgagg
     2161 acggggaaga cgttcttccg ggcgtaggcg atgtacttcc tgaggagctc cggctcgatg
     2221 gcggggcgga tcacctccat caccgcctcc agctcctccc tcgagcggtc cccccgggag
     2281 gcgatctccc cggcgtaggt ggtctggccg atgtggtggg cgatcatgct gtcccgggcg
     2341 tcggagggct cgtcggtgag gacgaagatc aggtcgaacc gcgacatcag ggcgggggag
     2401 aggttgatct gctgggctat cccctcgtac ttgtcgaagc ggccgaactt ggggttcgcg
     2461 gcggccagaa gcgcgcaccg cgacttcaat gtcgccatca cccccgcctt cgcgacgctg
     2521 atcgtctgct gctccatcgc ctcgtggagg gcggaccggt cctcggacct catcttgtcc
     2581 atctcgtcga tgcaggcgat acccttgtcg gcgaggacga gggcgccggc ctcgatcgtc
     2641 caccggccgt cgccgagctc gtccttgacg gcggttgccg tcagccccgc cgaggtggag
     2701 ctcttcccgg aggtgtagat gccccggggg gagagcttcg agatgtaccg gaggagctgg
     2761 ctcttggcga tgccggggtc gccgaccagg aggatgtgga tgtcgcctct tatcctcgtc
     2821 ccgtcgggga ggcccttgga gaccccggag aagagctgga gccccagcgc ctccttcacc
     2881 tcctcgtagc cgtagatgga gggggcgatc gaccggcgga ccttctcgta gatgaggggg
     2941 tcggaggcga gctctcttat cagccgctcg tcctcggcct tgatatcgat ctcctcgaac
     3001 tcctgctcca tcatctcgac ggagtttcct tcgaggaaga gatcgaagta cgtcgacttc
     3061 ccggtctggg tcgtcctctg gtaggaccgg aggacgccgt tgatgacgac ccggtccccg
     3121 gggtagatga tccctgagag gtcgtcctcg agctggacgt ccagggtctg gggctgctcg
     3181 ccgccccgga gctcctccgg cgactcctgg acccggacgt tctgggcgtc gacgaacctc
     3241 gacctgtcga ggaggagctt gaagggcccc cgccggtcgc aggcgtcgtt ggggcagtcg
     3301 tagggctcct ggaacttgga ggt
//
LOCUS       DASM01000061           19899 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_23739, whole genome shotgun sequence.
ACCESSION   DASM01000061 DASM01000000
VERSION     DASM01000061.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 19899)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 19899)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..19899
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_23739"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         3358 a         6501 c         6277 g         3763 t
ORIGIN      
        1 aatatgggtt tcattaaccc tcatacagtc gcagtaagcc ttgtcgccat ttgttttgca
       61 gtcggtggag ttagaacaaa gggcgtgcca tccggtacac accacaaatt cctggggtac
      121 ctcaataaaa ttatgggtga catcatccgt tttttctcca acatctgcca aacatatcgg
      181 cccataagtc attgctatcc aggttatgca agtgatcgct aaatattgaa atctcatatt
      241 ttcgcaccct ttccctcata ttatatcatt attaatggtg attgctaacc gataaacctt
      301 ttgtctctta cctatcgcgg ccctcttcaa agtctctatt ttcggctact gagatgatat
      361 gcggggccaa gcaaatttgg cataatattt tgacagaaaa tgcgagaaca ttaagggact
      421 caaggactga aatgagctga atcggggcat ataatcacct aaacttggac ctaaggacgc
      481 taaatcgcga cagtcgatgc tgtaggcacg accgcgagta gaagtgcgag ggtagatgga
      541 aaagtcgaat tattgaatcg gataaatata aaaagacaaa gaacgaggtg cagaaaaata
      601 gagcggtgag acgatgggcc ggtcctcgaa ccggcccccg cgcccgcctc acgcgaactc
      661 agcgaacttg ccgtatccct ccttcttgct cggcttcacc ttcttgaggg cctcatcgaa
      721 gtgcttcgca tcgatcctgt actttgcgat ctcctcctcc tcctggggcc ttccggcgag
      781 gacgtactcc cggatcgcca gcatcaccgc ctcgttgcag acgctggcga ggtcggcgcc
      841 ggagtagccg tcggtcctct tcgatatcgc cacaaggtcc acgtcctccg cgagcggcct
      901 cttcgcggtg tggatcttga ggatgtcgag cctcgcctcc tggtcgggca tcccgatctc
      961 gatcatccga tcgaaccggc cgggcctgag gagcgccggg tcgatgatgt cgggcctgtt
     1021 ggtggcggcg atgacgacga cgttgtggag cgcctccagg ccgtccatct ccgataggat
     1081 ctggcttatc accctctcgg tgacgtggga gtcgctggtc cctccgcccc gggcgggggc
     1141 gatggcgtcc atctcgtcga agaagaggac cgtcggcgcc gcctgcctag ctttgcggaa
     1201 ggtctccctc accgcccgct ccgactcgcc gacccacttg gagaggaact cggggccctt
     1261 caccgagatg aagttcgcct ggctctcggt ggcgacggcc ttggccatca gggtctttcc
     1321 ggtgcccggg gggccgtaga gcatgatccc cttggggggt gaggccgcca tgtggctgaa
     1381 gagcttgggg taggcgaggg gccactccac cgcctccatc agctcctgct tgacctccgc
     1441 aaggcctccg atatcatccc agcggacgtt cggcgactcc accagcacct cccgcatggc
     1501 ggagggctcc agctcccgga gggcgtcgac gaagtcctcg ttgacgacca cgatcttgtt
     1561 gaggatctcg acggggatgc tctccacgtc caggtccagc tccgggacga tcctccgtaa
     1621 ggccctcatc cccgcctccc gggcgagggc ggcgaggtcc gctccgacga aaccgtgggt
     1681 gatgtcggcg agcttctcca gtttgacgtc ctcggcaagg ggcatccccc gggcgtggat
     1741 ctggaggacc tcgagcctag cctcccggtt ggggacgccg atctcgatct ccctgtcgaa
     1801 cctgccgggc cgcctcaggg cggggtcgag ggagtcgggc ctgttggtgg ctccgatgac
     1861 gacgaccttc ccccgggact cgaggccgtc catcaccgcc aggagctggg cgactatccg
     1921 ccgctccacc tcccctgtca cctcctcccg cttcggggcg atcgagtcga tctcgtcgat
     1981 gaatatgatg gagggcgcct cctcttccgc ctccttgaag atctcccgga gcctctcctc
     2041 cgactcgccg tagtacttgg acatgatctc ggggccgctg agggtctgga agtgggagtt
     2101 cgtctccgag gctagggccc gggcgaggag ggtcttcccc gtccctggag ggccgtggag
     2161 gaggaccccc ttgggggcct ccacccccag ccgctcgaag agctcggggt gcttcatggg
     2221 aagctcgatc atctccctga tcttcttgat ctcaggcccg aggcctccga tatcctcgta
     2281 gctgatcctc gggatcgccc tctcctcctt cgccggcttc tcgctgatct cgatcccggt
     2341 cctgtccccg atgatgaccg cttcggcggc gggggtgatc ttcgccgcca cgagctctat
     2401 cttcctcccc atgacgctga tctcgatgac gtcgccccgg gtgaggaccc tcccgtccag
     2461 gaggcggcgg aggtactcct cccctccggt gatccggagg gggacggtgg gggcgaagac
     2521 caccttgccg gcgggagccg acttcgtcac cttcaccggc acccggtcgt ctatgctgac
     2581 gccggcgttc cgccggatgt tcccgtccag ccggacgatc cccgtccccg tatcctcggg
     2641 gtatccgggg aggaggaggg cggcggtctt cttcttcccg gatatggata gaacgtcccc
     2701 tgcctgccac cccgcgttgt atatgacggc ctggtcgacc ctcgcgatgc ctcttcccac
     2761 gtcggaagga cgagcttcac caactttgag cgtgtactcc tcagccatat caatcctcgt
     2821 acaatatcaa tatcttttta tttcaaccta tcctatcagc accatccggc tctcgtcccc
     2881 gcccggcctg ggcggccgct gctgttaggc atccaggccg gatcggcgcc tccttcgatc
     2941 ggccggggga atcttcaact cgcaacgtcc gtctatatgc agttactaac ttcaggttag
     3001 tacttagcag ttaagaagta tttaagcgtt tcgcttcacc gggatcttgc ggccgatcgc
     3061 gccgaaggag acggtgcagc tttatgtaga aggatcccgg cggcggcagg gacctgggag
     3121 gagctatcgc cccgcgattc ccgatcctga gaggggaggc ggcggctttc gatccggtcg
     3181 atctaccaaa agggaaagtt atcaagcctc gggtgggatt tgaacccacg gcctcgtcct
     3241 taccaaggac gcgctatgcc agctaagcca ccgaggcgcg aaaaggtgcg atcgcccctt
     3301 gcgtgctttc tgtttttgaa cctttcggtc cagccccgac cacccaaccc ctcgcctccg
     3361 agccatcgag ctccggctca ccccctcttc cagcctccgc caccggccga ccagcatgac
     3421 ggaagggtta tttatctcat ggggaaccaa atccggcccg tgtcgccgaa gctccagaag
     3481 ggtgaagaga tgatccgggc cctgaggccc caccctctgg ccttctaccg gctctacggg
     3541 atctggatct acatcgccct ggtgggggtc tacttctcct cctaccccgg gatccagatc
     3601 ttcgatatga gccccgagaa ctcggcccgg atcctctggt ggctctcgat cctagtcccg
     3661 gcggtcctga tcgccctctt tcggatcaac tggcggtggc tcgccgccct gggggcgcct
     3721 gccctcctcg ccttcggcct ccccggcagg ccggagtaca tcgccctcct ggaggggtcc
     3781 ggtctcgcct ggtttatgga cccaaaccac atcctcacca ccttcggcct cctcggagtt
     3841 ctggggacgg aggggcaccg gaggtctcac cgctactaca tcaccaactg gaggctgatc
     3901 atggagtcgg gccacctggg ggtccaccgg aggaccctcc tttacaagaa cataaacgac
     3961 ctggtggtgg agcagtcctt ccccggaaag gtcttcaact tcgggaccat catccccatc
     4021 accgcctcgg gcctcggcct cggggacgac ttcagcctgg cgggggcggc ggtggggggg
     4081 agggcgatgg tgggggctgg cggtggcaag gccgtatcga ccccccgggg tcggtcccac
     4141 cacctcctgg agggggtcgg ccggccggag gaggtccacg acctgataat atccctgatg
     4201 cactctcagg ggtgatgggg gataaaaggg atgatctggg agggcaaggg ggcggaagtt
     4261 cgggtccgct acccgcatct tcgttaatta acctctatcc agaaccctct tgtagacctc
     4321 ctccgtctcc cgggcgatct cgtcccaggt gaactccctc tccagcctct ctcgcccggc
     4381 ccggccgagc ctctccatct cctgggggtc gtcgaggagc cggttgatgc accaggcgat
     4441 cgactcgggc tggatgtaag cgaggaggcc atcctcgaag ttcttgatga tgctcaccgc
     4501 ctcggtggct atcacaggct ttcccgcgtc ccacccctcc aggacgacga tcccgaaggg
     4561 ctcgtttcgg cttgggaggc agaccatgtc gcaggcgttc atccactcct cttttacgtt
     4621 gctggggatg tagccgagga agatgcagga accttcgacc cccagctcgc gggcctgccg
     4681 ctcgcactcc gccctcatct cgccgtcgcc gatgaagacg aacttggcgt cccagtggcg
     4741 ggcgaggatg gagggtatag cctcgactag gatgcagggc cccttctggt agttcatcct
     4801 cccgcagaag aggacgaccg gcgcgagggg gtggatcccg tgcctctcct tcacccttcc
     4861 gggatcgacg gaccgcctga aggagccgat gacgaggccg ttggggatga tctctatctt
     4921 ctcggggggg atctggtaga gcatcatcaa ttcgtccttc atcctctgag tggtggtgat
     4981 caccatcgcc gcctcgtagc cagcgaccca ttcccggtgg gcgatctccc gggggatgta
     5041 atcggtgctg aagttgtttc cgcacctccc ccattcggtg ctgtggaggg tgatgacgta
     5101 ggggatccga taggcggcct ttatcctgtt gagggcggtg accgggtgcc agtcgtggcc
     5161 gtggacgacg tcaaacttac caaagagctt ctggacgtgc cagaaccggt cgaagatggc
     5221 gtcggacatc cggtccatct gggcgaggat atccccggcc gggtcggagt cgaccctctg
     5281 gtagtggacg ccgttgatct catcgtagga gccgaagtcg cccctccggg tgaagacgtg
     5341 gacctcgtgc ccccgccgcg cgagagcttc ggagagctcc gagacgtggg gggcgacgcc
     5401 cccgaccttc gtggagtaga ggctctccca ggagagcatc ccaattagca taagttcacc
     5461 aaatcaagat ccggtagctt ttggtcgctc ggccgtttca ggatatcaag agcccccggc
     5521 cgctaaaagg cggcccggcg agggcgatag gcccctgggc atcgatccgg gctcaaatga
     5581 gcatccagtc ggataatttc gctgcaattt cttattctgt tgtctcatta acctttcggt
     5641 ttttcccttt cttttcggcg ggtagacggc tggctgggat ccgatggctc cggcctgggc
     5701 cctccttccg gaggggcggg gccggaggag gactctagac cccccggagc tcccgggccg
     5761 cagcctcggg ggggacggag ttgatgaaga tgccggtgtt cctctcaaag ccagccaggg
     5821 tcgatatcgc cgggtggatc ctgaggttta agtggtagcg gcggtcaggg agctggcgga
     5881 tggtatagtt gtaggcgggg cccgagagga gcctgtcctg cctccggagc gcctccccca
     5941 gcgcctcggc cagatcctcg agcccccggg ggtcgagcgc ctccagggac ggggcgtgcc
     6001 ccttggggag gatccaggtc tcgtaggggg cctccgagaa gaagggggcg atgaggacga
     6061 accggccgtt ctcgaagatc agcctatccg aggccgcctc ccgctcgacg aggctgcagt
     6121 aggggcatct cggggacctt ccgatcgcct ccagctccct caggagggac ggggggacga
     6181 ttggcagggc gacgagctgg gagtgggtgt ggctgagaga ggcccccgcc acccgccccc
     6241 agttcttgaa gatcgagacg taccccaccc ccggatcggc gatgcggcat gtgtatctat
     6301 ccctgtagac ggtgacgagc cgctcgagct cctggcggct gaactccgat gggtcggcgt
     6361 cgtggtccgg ggactcgatg atcacctcgt ggaacccccg ggcgcggtct gcaacccact
     6421 ccctcgtcgg aggctccggg tccgccgtca ccgccggata caggttggga aaggaccggg
     6481 cccaccagtc cctgaccctc tcgaccccgt cgggaccgac ggcgtcgtcg gtgtaggcgg
     6541 cgaccgcggg aggggtcatc tcctcgtttc cggggcagaa ggggcaggtc ttcgggtctg
     6601 acctctccct ccccctctcg cggcggaagt cggagggcct cttcctcctc tcggcggcga
     6661 tgatgcagta ctcctcgagg aagtagtggc gcctcatctc cgggatgaga tcaccttctc
     6721 gtagatctcc agggtctctt ttgccgcccg gggccagttg aatatatcga ggcacttggc
     6781 cctgccgttc ttgccccatt ccctcatcct ctcggggtct tccaggacga ggtttatccc
     6841 ccaggcgatg tcgccgggat cgtgagggtt gacgtgaacc cccgtgggcc gctccgagga
     6901 ggggttgtcg acgatctccc ggaggccgga gacgccgctg gctccgacca ccgccggcct
     6961 ctccatggcg gcggcctcca cccccacgat cccgaagggc tcgtagaggc tcgggaagac
     7021 gcagaggtcg gcgagggcgt agtggtggat cttcgactcc aggtcgagaa agtcggtgac
     7081 taccttcacc gatccgccta gggcctctgc catcgacctg accctcccct ccagggtacc
     7141 cttcccgacg acgaggagct tagcccgagg gtgctcctga aggaccttcg gcatcgcctc
     7201 caggaggggg atcaccccct tcaccggctc gagcctcccg acgaagagga tcacctcgtc
     7261 ctcctccttt agggagtacc gctcccggag ctcctcgatc ctcgtcctct tcgtcgctga
     7321 ggggctgaag acctcaggct cgacgccgtg gtaagagacc tcgatcttat cctccgggac
     7381 cccgaggccg gagagctgct ccttcatcgc cttggagacg gtgatcaccc cgtcggcgag
     7441 gccggcgccc ttcagctcca ggtcgacgat ctgggggttt ggggccttct ccctccccac
     7501 ctccaggctg tggacgtgga agatgagggg gacccccagg accctcttcg ccgcgaggcc
     7561 gccggggagg ccgagccagt cgtgggcgac ggcgaggtcg aaggggccgt tctccctcag
     7621 ggaggcgacg gcaagctggt tgtagctcaa aaggtcgaag aggaagtcca ccccctcccc
     7681 ccaggcgcgg gtcctgtcgg agatgaaggg ctcccagccg tccctcatcg gcgccggcgt
     7741 ctcacggaag acggatattc ctcgggcctt ctcccgcctc ttgagggagc cgtcccccat
     7801 ggtgaagacg gagatctcct ggccgaggga tgccagctcc ttcgatatgg agtcggcgta
     7861 gacgccgagc cccccgaaga tccggggggg gaactcgagg ctatagtaag ctatcttaat
     7921 agaatcaccc caggtcgaag cctctaatct cgggaggcat gcacttgtcc agctctgagc
     7981 tgaccatcag gttcttgtag tactccagga ccggctcggt ccaattcatc agcttcgcca
     8041 gggcgaagga ctcctcgcag aggagccggt acttcacctg gtcttcgagg taggtccagg
     8101 tgaagtagtc gagggcgagg gcgtagtcga ggatcatctc ctggagggac gcctcgatgt
     8161 tgtcgacgac cacgaccccc cccaggccgg tgaccagccg ctccaccttc ttcagggcgt
     8221 cgctgaagcc gcaggcccgg ctgacgatgc tgggggttcc ctcccgcccg gcctcgaggc
     8281 cggtgaggag gaagggctcg tactgggagg ggaatatccc ggcgacgcac cctgccatga
     8341 tctcgtcgac ccccatcttc atcccgccgt cgtcggggcc cgcccactgg gggtagagga
     8401 tggcggagac ctgcctcctc ccggcggcga ggtttccggc atcgatccct aggtcccgga
     8461 tcatcttgtt cagcctgatc tcctccccca ccaggacctc ggctgggagg tcgatgggga
     8521 actcctcggg gagcttctcc ttggacttcg ggccgtggga ggtgatgagg aggcagacga
     8581 ccctcatctt ctcggcgaac ctgtggtcca tcctccggtg tcttaagagg tggttctgga
     8641 ggaggaggga gtcgaggagc tgggggtagc ccttgttctc gagctctagc ctcgatatgg
     8701 tgaagacggg aagaatctcc tccgggtcga tcacctcccc gccgtagacc cggtggagcc
     8761 cctccgagag gaacctctgg atcttcctcc ggcaggcgtt cttcttatcc cagtcgatcc
     8821 gtctgacgtc cagctcgatc ccgtttctta tgacgatccc gttcagtttg tgaaatagca
     8881 tcgcctccct catcgtcgag tcgccgacga aggagacgac gtcggcgtac ttcgccaggg
     8941 actcgagggt tgccatcccc accgggacgt tcgggtccca ggtgccgtcg ttcttcgcca
     9001 ccttctccag ggacctgtaa ccggaggacc tccccggcac cgtggcgtgg agggtggcca
     9061 tcgtccggac ggggacgccg aggcacttca gccgcgccag ggcgtagaag gtgggaaact
     9121 cgtggcagtg gagggatacc cgcatcttcg gcatcaccga cctcgcgaac tccgagatcg
     9181 cctcgtcccc gtacttcttc gccctctcct cccccaggga tacgaggtag tggacgaact
     9241 gggagacggc gtaggagagg ccgaggtagt gggtgtactc cggtccgtag ggggcccgtt
     9301 caaactgcat cgagtcgagc ctcgagagct gccacgcctc ggccttgatg gcgttcgcga
     9361 gggtcatctt cgtcccgtac agagtcttga tgatcgagtc gtagtaagcg gtgttgaaga
     9421 ggaggtagcc gatggggacg ccagccacct ccgtctgggc agccacgaca tcgatcccct
     9481 cggccttgag ctggtcgagg gcggatgtga gctcgtcgtt catatcgagg gcgtcgagcc
     9541 ccgagacgtc ggtgaccctc gccttcccct tgttccagtc cgccccggcg gtcgggtagt
     9601 aggggccagc gacgaggatc ttgaggtcgc attcgatctc cttcctggcg gcgagcctcg
     9661 ccagggtcct cgcctcggcg tcgatgacgt cccatatccc gcccattttg ttgcagacgg
     9721 gccctccctc ctcccccgcc aggactatcc agcgctttat catgacgaat ccctcctcgc
     9781 tgacgtctaa ggatagggcg atagggtcga gaccgccatc ccctcgtcgg atatgtccag
     9841 gacccggatg ctgttcgata tcctggagcc cctcatcttc atgatgtaga tcgatcggac
     9901 catgttgttt ccgatcctct ccctcttgag gatggtgcag gagtccgccc cgtactccat
     9961 gatctgggtc atcccgaagg cctcgcctat ggtgatcacc gccgtcaccc ccagagaccg
    10021 gaggtcgccg aagaggtcgt cggcgaggga cctcatgacg aagggggact cgacggcggt
    10081 gaagaaggcc atcaggtcgt cgatgaggac cctctggggc ttctcctccc ggaccttctt
    10141 gtcgacaagc tttctaaaat ttatcagaaa ctcgacgggg tcggtctcga tgctcttctg
    10201 gagcctcagg acagggtcgg tgacgtcgac gatgacgagc ttcccctcct cctccagctt
    10261 ccagaagtcc caacggaagg aggagttgag ctcccgcttc aggtactcgg cgctctccga
    10321 gggcataatg atcatcccct tctcgccgtt cttgatcccc tcgtggatga actgggatgc
    10381 gaagacggtt ttgcccgtcc ccgaggaccc agtgacggtg ttgacggtcc tccggtgaaa
    10441 gcccccgttc accaggtcgt cgaagccggg gatccccgaa gggaccttct cgtaagattt
    10501 tgatgattcg tccacttttt tttcccctcc gagagtcctt attcgaccga gagtaattat
    10561 ttttatcggt gaagggcgaa agattttcgt ctccaccggc ccctaagacg gcgatcttct
    10621 cattccgatc cctcagtact gctcatagac ccacgacctc tcggccggga agagggcgtc
    10681 gatgaggcga acggtcttat ccgaggccga atccacgccc ggtccgacgt acatcatcgc
    10741 cgggaagagg tcgggccgga tgtcctggag ctctcgccag gtccgatggc cgaggaccag
    10801 cggagcgaag aggtcttctg gaatgaagaa ggccttctcg cattctcctc ccctccccga
    10861 ggtgacggat tcgagcctcc cttcagacca gcagagatcg atctgttcgc aataaaggtc
    10921 gaggcgcaag gtcccggtga agcccgcaag gctgctcttc ttcagccgcg cctccaggac
    10981 gggggcgatt tttccgagga accttcctcg atccgggatc tttatctgcc aggcgtaggg
    11041 cctccccacc gacgccccga aggagatggc ggtcttagcc gcggtcgact ctatggcgag
    11101 gttcagccgg atgtacggct tatttcgctc tgcgcacatc ttcttgcaga aggcgaaaag
    11161 gttcgtcagg gcgtcgtcgg atatcccctc gctgacctcg ctcacgatga gccccgctcc
    11221 gaagccctgc cgggagatcc gaaagtagca tctctcttcc ttctctcgcc cttccatgat
    11281 ccagaactgc gagccgcttc cgatcctcgg gctgtaggtc agaagatact tccagtgggc
    11341 ctcgtccctg aagacggaga tgaagttcga tcttcgatac ccttcgtcct cccccaggag
    11401 gaagggtatg tcgccctcct cggcaagccg gaagtcgtag gctccgccgt cgccaccgcc
    11461 ggtcgaatag gggaccagat ggagggggat gtcgatgtgg ggctccaggg ggatcgagta
    11521 ataatatccg aagcggcgat aaaatccagg tataccctgg acgacggcga ggtcgaactc
    11581 ctcctcagcg agggtcgcgt cgaactcccg gtacatgagc ctcataaggc ccttgttacg
    11641 gtgtccctct cccgttccca cgatccccat ctcggcgact ttgagcctcg tccccgccat
    11701 ctcccaggtc caggggatga gggcgaaggc tgaggcgatc tcaccggtct cctcctcggc
    11761 ggcgatgaac cagcgctcgt tcttcatccc cggaaggtgg gagaataccg tttcagcgaa
    11821 ctctcccacc tcctcgggaa gaaagatttt gtcgaaatga tggcggagct ttccggcgtc
    11881 gtcgggagac tcggcccttc ttattctgta atttgagtcc atggaatcga ttacctcgca
    11941 atttttgatg tccatcatcc accttgaata ttccgccgat tgaaggcctc cttccgagcc
    12001 gggagtaccc gaggctcgtc gaacaaatat ttattgaact tgatggccaa tatatggtca
    12061 tgtcatcgat cccgcccctc acgtcccgct caggttttct cgatgccgac gaagacgctg
    12121 ccggtcccgg tgaggcctca tgaccgacgt ctgcctctgc tttgaggtcc accagcccct
    12181 gaggctgcga aaggacttct tctggtccgg atcgcccatg aggaggatcg agcgagacct
    12241 gctatccgac ttctacttcg acggtccgga gaaccggcgg atattcgacc gggtcgccga
    12301 gaagtgctac cttccggcaa accaggtgat cctcgaggag atcgagaggc accgggactc
    12361 cgaccgcccc ttcaaggccg cctacagctt ctcgggggtc ttcctcgacc aggccgagag
    12421 gtacaacccc gagatcctcg actccttcgc aaggctcgtc gatacgggga tggtggaggt
    12481 tttggagcag acctaccacc actccctcgc gagcctctat gaggacggca aagagtttgc
    12541 cgagcaggtg aggatgcacc gggagcgggt ctgggacgcc ttcggcacca ggccccggat
    12601 cttcgagaat acggagctgc tatacgacga ccggatcgcg aagatcgttg aggggatggg
    12661 ctacgccggg atcttcgccg aggggatcgt cgcccatccc aactgggtct atcggccgga
    12721 ggggtgcgac cggatctcgc ttctcctccg ggactatcag atcactgacg acatggggtt
    12781 tcggttctcc gcccggtggt ggaaggagta ccccctcacc gccgagaagt acgcctcctg
    12841 gatcgcggcg aaggtcggcg actgcgtcaa catcttctgc gactacgaga ccttcggaga
    12901 gcaccagtgg gcggacaccg ggatcttcga gtttctcagg caccttccaa ggaagatcct
    12961 ccggtgggag cacctccggt ttgcgacccc cagcgacgtc gtccgggacc acccccccct
    13021 ccagaccctc tccgtcccca ggaccacctc ctgggcggac atcgagaggg acaccagctg
    13081 ctggctcgga aacgccctcc agtgggcatg ctactcctac caccggcggc ttgaggggcc
    13141 gataaaggag agcggggaca tgagccttct ggagatctgg aggaccctcg gcatctccga
    13201 ccacctctac tacgccttca cccacggcgg cgccccgggg gaggtccaca actacttcag
    13261 cccctacggc aacccctatg acgccgccgt caccttcttc gccgtcctcg ccgacctcaa
    13321 ccaccgggtg aagtcgtggg cctccgccgc cgaggacccc ttcctcttct cgaccgggat
    13381 cgaccagttc accggcgacg tcgtctgggg gccgaggggg ctcgccgagg ccttcgagcg
    13441 ggtggaaaga gaggccctag agtaccacgc gaggcggggg gacctctccg cctgggcccg
    13501 ggagagcctc accgaccccg ccctcgccga ggggctcgag cgggccgaag gcctccgggg
    13561 cgaccccctc cgggccgccc tcctggaggc ggcggcggag gcggtcagga gggccggccg
    13621 ccccggagct tctgggggcg gcggggcgat ggagccgatc gaccggagcg ggaggaagag
    13681 gagatgagct tcgacctctc tatcagaccg aagagctatc aggagggctc ggagcgggag
    13741 tggctcgtca ccaacggcct cggagggtac gcctcctcga cggtcatcgc ggcgaacacc
    13801 cgatcctacc acggcctcct ggtggcggcc ctgacccccc cgaccggaag gaccctcctc
    13861 ctctcctcca tagacgagga gctgatcgcc gacgggatca gctgccacct cgcctgccac
    13921 cagtaccccg ggacgatata cccccagggc ttccgccacc tccaggagtt ccagaggtat
    13981 ccggtcccga ccttcctcta tcggaccgag gggatggaag taaaaaagag ggtcttcatg
    14041 gtctacggcg agaacaccac cgtcatcaga tacgatatcg aggggatcgg cctcttcagg
    14101 gccatccccc tcgtcagctg ccgctccttc cacgtcgcct ccggcgcccc tcccatggac
    14161 cagtcgcgcc tctcggtcga ggggcccgga gggggcgtcc gcctccggtc ggattgctcc
    14221 ttctacatcg tctccgacgg ggcccgctac ctccgggagg agctctggta ccacagcctg
    14281 gagtacgagg tcgagaggcg gagggggtta cagtggcggg aggacgcctt ctcccccggg
    14341 aggttcgagg cggaggtcga cggccgcctc tccttcagca tcatcgcctc cctccaccgg
    14401 agctccccgg agggggcggg ggcccttctg gtccgggagg aggagagggt caacaggctc
    14461 ctcgcgacgg gcggagatcc gcggtcagcc gtcctcgcct ccagcgccga ccagttcctc
    14521 gtccggaggg gcgacggcaa gagcatcata gccgggtacc cctggttcaa cgactggggg
    14581 agggacgcca tgatcgccct ccccggcctc ctcctcacca tcgggaggtt cgaggacgcc
    14641 gggacggtcc tcgccacctt cgccggggcg atgaaggacg gcctcctccc caacgacttc
    14701 ggggccgagg ggtacaacac catcgacgca gccctctggt tcttctgggc gatctacaag
    14761 tactggtctt actcggggga cctggacctg gttcgccggc tcctccccac cctccgggcg
    14821 atctgccgcc gttacgccgg ggagacccca ggatcttact ccgacgggga cggcctcatc
    14881 gcctccaagc ccggcctaac ctggatggac gcgaaggtgg gggaggagtt cgtcaccccc
    14941 cgggcgggga aggcctgcga gatcaacgcc ctctggtacc acggtctgaa ggtgatggag
    15001 ctcttcaccg agaggctagg cgacgaggcg gtggaggtgg gggtggaggg ggcggacgag
    15061 gtggtctacc ccgatctggc ggagcgggtc ggcgagagct actcgaagtt ctggaacccg
    15121 gaggagggct gtctatacga cctgatcgac gggatcgacc ccgccatcag gccaaaccag
    15181 gtgatagccg cctccctccc cttcaccccc ctcgactccg gccagatccg ggggatcgtc
    15241 ggcgtggcga ccgaggagct cctcaccccc tacggcctgc ggaccctatc gccgagggat
    15301 ccggcctacc tggggcggta cgagggcggc cccgccgaga gggacggggc ctaccaccag
    15361 gggacggtct ggccctggct gatggggcct tacatcaccg cccgcctccg ggcgggcggc
    15421 aatacgaaga aggagaggga ggcggccggg gatctcctcc ggcccctcat cggcctggag
    15481 gggcagatcg gagtcgggac gatctgcgag gtcttcgacg gcgacgggcc ccaccggccc
    15541 ggaggctgca tctcccaggc ctggtccgtc ggcgaggtga tgagggcgtg gcacgagggg
    15601 gttctgggcg gggcgagggg tccgaaggtc tgacccctgc cccctcctct tcccggaccg
    15661 ctgttcaggc cccgctgggg gggggggcgg cccgccctga tacatctact ccgggatcgt
    15721 cccttccgag ggctggggga tcatcatccc catcagaagg tcgagccagg agggggcggc
    15781 gagggcctcc tcgggcacct ctatgacgat cggctcgttg taccgggagt aggtcaccgt
    15841 cgattcggac tcgacctcca tctcgatctc cgggctctcc ccgccgggga ggttcatcat
    15901 atcagaggtg atcgtcatgt ccagggaaac atcgctcttc agggggaggg cggagtcctt
    15961 ggagatccag gtcgtccact ccatctcgct ctcctcgaag atgtcggtga ggttcatggc
    16021 gtatatcggg agggatccga cctgctggct caggatcagg gtgaaggtct ccatatccgg
    16081 cgccaccctc accttgtatg catcgatccc gccgaccttc tctgagccga tgaactcgat
    16141 ctcggaggcg ttgaggagtt ccgcctgggt cctcacctgg ttctgctggt cccacatctc
    16201 cggcggcatc cccggcatcc tcgtccagtt gccgtcgatc ttggtgtaga tggtgtcgtt
    16261 ctccatgtac atctccatct ccgagacgac ctctccgagt tcatcggtgc tggtggtggt
    16321 ggtcatcgtc atcttcagcc gccgggccga tacgttcatc accccctcac cctcggacct
    16381 cgtctcgaag ttgatcgtcc tcagggatct gttcgtctcc tggaggttga cgagctttat
    16441 cttctgatcc gtatccacgg cgaaggcggc ggtctcgacc ttccctaccg actcgatcat
    16501 catcgccttc agctcctccg cgctcggggt ctcctcgacg cagccggaga aggctacggc
    16561 gaccagggcc aataacagta atagtttctt catgcgggag gccctctctc cgggggacta
    16621 tttcatggta tcgttcggct cgggcgcctc ccaaaagcga ggatcctctc tcatcctccc
    16681 caccccatcc ttctgaccct aagcccggcc ccggaggcgg gagcgggcaa tctttatata
    16741 caacgtcgat attaagacac tatagtacaa aatcatacat catggtgatc atatgaagca
    16801 tctccaacag gtcttggagg ggagggatct caccgtctcg gaggcggagg ctctcctcgg
    16861 cgaggtcttc aacggggcga cggacgccca gatcggggcg ctcctcgtcg ccctgaagat
    16921 gaagggtgag gcggtctcgg agatcgcggg gctcgcgagg gctatgaggg ccggaggggt
    16981 gaggatcagg ccccgggtac cgggccccct cgtcgacacc tgcgggaccg ggggcgacgg
    17041 gacgaacacc atcaacgtca gcaccgccgc tgccatcgtc gccgcctcct ctggcgcctt
    17101 tgtagcaaag cacggaaact atgccgtcag ctccgcctgc ggctccgcca acgtcctggc
    17161 ggagctgggg gtggaggtcg cccccggccc cgagcgggtc tgccaggcga tcgagaccct
    17221 ggggatcggg ttcatgctcg cccccgtctt ccacccctcc atgaagcggg tcgcctccat
    17281 ccggagggag ctagccacga ggacggtctt caacatcctc ggccccctca ccaaccccgc
    17341 gggggcgccg ttccaggtga tgggggtcta cgacccccgc ctctgcgata agctcgccct
    17401 cgccctcaag atcctgggaa ccgagagggc gatggtcgtc cacggctccg gcctcgacga
    17461 gataaccaac accggtccga cccggatctc cgagctggat cggggcgccc tcaaaagcta
    17521 caccgtcacc cctcaggagt tcggctaccc cctggtccgg atcgaggaga tccggggggg
    17581 gagccccgag gagaacgccg ggcggctcgt cgagatcctc tcgggggcga ggggccccgg
    17641 aagggacgtc gtggccatga actcgggggc tgccctctac gtcgccggga tcgcggagtc
    17701 tctcttcgac ggcgcgagga tggccgagga gtcgatcgac tcggggcggg ccctcacgac
    17761 cctaaagagg ctcgtcgcct cctgcggcag ccccgagagg ctggagcggt tcctatgacc
    17821 agggcgaaga tctgcgggat cagggacccg ggggccaggg acgccgccgt cgcctcggga
    17881 gccgacgccg tcggcttcgt ggtggagatc ccccggtcga ggaggtcgat cgggcgaaag
    17941 gaggcgaaga gcctcatcgg ggggctaccc cccttcgtct ccagcgtcat cgtggtggag
    18001 ccaaaatccg cggcggaggc ggcgagcctc gccctcgata cgggggccga agtcctccag
    18061 gtgaacggga ccctcagctt cgaggatctc gaagccctcc gggagatggt ccccgcaagg
    18121 ctcgtggcga cggtcccggc gaggtccggg gccttggagg aggcccggag gctcgccggg
    18181 gtcgccgacg ccctcctcgt cgacagccag gaggggggaa ggctcggtgg gaccgggacc
    18241 gtccacgact ggagcctcac cgccgaactg gtgaataagg tcggcgcccc cgtcatcctg
    18301 gcgggcgggc tatcccccga gaacgtcgcc gaggcgataa gggccgtcgg cccctacgcc
    18361 gtcgacgtct cctctggggt cgagaccgga gggaggaagg acccggcgaa gatcgccgcc
    18421 ttcctcaggg gggtgaggag ttgccctccg cggtgaggtc cttccccgcc gatgggggtc
    18481 aggccccgga gatcggccct ctcccaaggc tcttcgaggt gacgtccctc ctcgaccccc
    18541 gggcccccct cagcctctac tccgccctcc ggggggaggg gtaccccttc ctcctggagt
    18601 ccgtcgagaa gtcggggcag agggcccgtt tctccttcgt gggggcgagc cccgccgccg
    18661 tggtgaaggt ccgggggagg aggtttgagg tccaggtctt cgacggcgcc ggaggtctta
    18721 tagagctcct caggaggagg ctcctcgcct ccgccgtcat cgacggcgcc ggaggatacg
    18781 gcatctccgg cgagatcagg cccgaaagag acctcttcga cctcctccgg tccgccatcc
    18841 ccgccgggac gggcccctca aagttcggga ggcaggcctt cctcggcgga gggatcggct
    18901 acctcgccta cgacctagtc gcggagagga tcgatcggcc caagacctcc gataagcccg
    18961 atgccgtctt cgggatcttc gataagagct tcgtcttcga ccacctgacg gggaaggtct
    19021 gcctcgccgt ctcccccctc ctcccggggt tggaccccga ggagatcgcc tctgccgcga
    19081 cggaccatct cggggacctg gacctccggg agccggggcc aggagacctc gatcccctat
    19141 cggtggaggc ggaccccgcc ggccccttcg aggaggcggt ccggagggcc aaagagcaca
    19201 tcctcgccgg cgacatcttc caggtggtcc tctccaggag gacgagggtc cggctcggga
    19261 ggcccgaccc cgtagtccta taccggaggc tccgggagat caacccgagc ccctacacct
    19321 acatcttcga gttcggggac cactccctgg tgggggcgtc ccccgagacc ctcttcagcc
    19381 tcttcgaccg ggtcgtgacg acaaacccca tcgcagggac gtgcccccgg ggcgggagcc
    19441 gggaggagga cgacctcctc gcggcgaaga tgctggagga cgagaaggag agggcggagc
    19501 acgtgatgct cgtcgacctc gggagaaacg acgtccggtc cgtctcgaag gcgggctcga
    19561 taaaggtgga ggagttcatg gccgtcctcc ggtactctca cgtccagcac atcgagacga
    19621 cggtccgggg taccctccgg gagggatgcg actccttcga cgccgcccgg gcgatcttcc
    19681 ccgcggggac cctctcgggg gcgcccaagc tccgggcgat ggagatcatc gacgacctgg
    19741 aggggcggga gagggggatc tacggcggcg gcgtcggcta cttctcctcc gacggctccg
    19801 ccgacttcgc catcgccatc cgctccgtcg tcctcgaagg ggacttggcc gtcgtccagg
    19861 cgggggcggg gatcgtcgcc gactccgacc cccaccggg
//
LOCUS       DASM01000062            3100 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_230377, whole genome shotgun sequence.
ACCESSION   DASM01000062 DASM01000000
VERSION     DASM01000062.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_230377"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          503 a         1056 c         1017 g          524 t
ORIGIN      
        1 ccggagaact ccctcccgcc gtagaaggag gcctcaaacc aggagtcgcc ctccggggtg
       61 gaaatcgaga ttttgccctc ccccggcccg tccctcctct tggcgtcgat ggctacgacg
      121 caggcctggc tcccgtactc cccggcgatc tcggatatga ggccaggccg cttcagggcg
      181 gcggtgttga cggtgacctt gtcggccccg gcctggaaga ccgcccggac gtcctcgacg
      241 gccctgatcc cgccgccgac ggccaggggg atgaagacgt tcttggcggt ggccctgatg
      301 acgttcttca tcgtctcccg cctctccgag gaggcggtga tgtcgaggaa tacgatctcg
      361 tcggccccct cctcatagta ccgggtggcg agctcccagg ggaccccggc gtacctgatc
      421 tccttgaact cgatcccctt caccacccgc ccctggggga cggcgaggtc gcagtcgagg
      481 cagggtatta tcctcttcgc taacatcggg cgagcctccc gaagttcctg aggatggata
      541 gccccttctc ccccgacttc tcggggtgga actggacggc gaagaggctc tccaccgata
      601 tcgccgcagc gatcttttgg ccgtagtcgc aggtggcggc caccaccctc ccatcccggg
      661 gaaggcagtg gtaggagtgg acgaagtaga agaagtcgtc gtcggagatt ccatccagga
      721 tctccacctc cctctttatc gagagggtgt tccaccccat ctgggggacg aggaccgagg
      781 ccgggaggcg gacgacctcg ccatcgatcc atccgagccc ctcctcaggg ctctcctcgc
      841 tcccctcgaa gaggacctgc atcccgacgc agattccgag gatcggcctc ccctcgtcga
      901 aggcctcccg gagggcaggc tcgaagcgag agagctgcct catgcacctc ccgaaggacc
      961 ccaccccggg gacgacgatc ccatcgaaac tcgccagctc ctccggctca gtggctatct
     1021 tcggccccgc cccgatccgc tccagggcat tgtaaatgct gaagaggttt ccgacgccgt
     1081 aatcgactac tccaatcgtc aagtctcgtt cacccgatgg cgcagatcaa gatctaaagc
     1141 ctcgccggaa gccccatagc ggccccggcc atatatacct tttcggacgg ccctataaat
     1201 attaaattca ttccgcccgc atcatccggg tccccttttt agcctccccg ccaccatctc
     1261 caccgtcgcc cgctccgcct ccgcgctccg acggcaggtc ggacaggccc ggacccattc
     1321 ggggttctgg gatgggggga gggaagcctc gaccctccgg agcatcggct cggtggcgta
     1381 gcggcggccg cagatcctgc aggcgacgag gtcgaaggac gcctccggct ccggaaccgc
     1441 ctcatcgaag gggacgaggg agagagccct ggcggggcag tgctccacgc agaggtcgca
     1501 ctcctctcgg cacctcttcc agaggatcct cctctcctcc gccgtctccg tccggaggat
     1561 gttctggctc gggcagacgt tggagcagga gaggcagccg atgcagaggc ggagatcgat
     1621 ccggatcatc tccggccccc cggggcgacg gcccgagccg cccgggaccg ggcccggtgg
     1681 gagaggagat cgaagtcggt ggtgagggcg ttggcgggac aggtggtgac gcaggtcggc
     1741 tcggcccgct cctcgcagag gtcgcacttg tgggcgatct tctcggtcca gaccacccca
     1801 aaggggcagg ccaggctgca caatccgcat cctgtgcact tctccacctc gaaggccgtc
     1861 cggctcccct ccttgtggag ggctccggag aagcaggctg cagcgcagga ggcctcctcg
     1921 caatggtggc agagtatggg gacggaggcg gagtctccga cgaagtggac gtggacgtgt
     1981 ccccggcctc cgtggacccg ctcgcaggcc acctcgcagg agcggcatcc tatacatctc
     2041 cttatgtcta cgtaaactgc cattcaatcc ctccgaacta tcctgcaggc ggagaccttg
     2101 aactccggga tcctcgcctc ggggtcggtg gcctccgagg tgaggcggtt cgtcccgggg
     2161 aagtggaagg gcatgaagac caccccccgc ttcacccggt ccgtcaggcg ggctagggcc
     2221 tcggcccctc cccggggcgt ctccaccagg accgggtccc ccccggcgat ccggagggga
     2281 aaagcgtcgg cggggttgat ctccacgaat aggtccggct cgcgacggag gagggagggg
     2341 ctcctccggg tcatcgaccc ggcgttgtgg tggacggcca cccttcctgt ggtgaggagg
     2401 agggggtact cccgccccgg tccctctgcc gccggccggt atcggacggg gacgatggag
     2461 gcgcggccgt cggcgagctt gaagccggcg gagtggagga tcggggttcc ggggtggtgg
     2521 gggtcggggc agggccagat gagccccccc tcgccgagcc tctcccgggt tatgccggcg
     2581 tactggggta cggtccggtt gatctctgcc aataccgagg cggcgtccgg agagccgagg
     2641 tctatgccca gagacctgcc gacggcggct atgatctcca ggtccgaaag ggcctccccc
     2701 ggaggatcta cggccttcga gctccactgg acccgccgct cggtgttggt gaagctcccc
     2761 tccttctcgg cccagaccgc ccccggaagg acgacgtcgg cgagcctggc cgtcgccgtc
     2821 atgaatatgt cctggaccac caaaagatcg agatcttcga gggcctggtg gacgttctgg
     2881 ctgcagggat cggagttggc ggggtcctcc ccgaggatgt acatcgccct gatatccccg
     2941 gaggcggcgg cgcagatcat ctccgtcgct gtgaggccgg ggcccttcgg cagagccggg
     3001 accccccagg cggaggagaa gcgccgaatc gtctcggggt cgtcggacct cccgtagccg
     3061 ggatagaact ccgcgagggc ccccatgtcg caggctccct
//
LOCUS       DASM01000063            5708 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_16825, whole genome shotgun sequence.
ACCESSION   DASM01000063 DASM01000000
VERSION     DASM01000063.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_16825"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          979 a         1870 c         1756 g         1097 t
ORIGIN      
        1 cgaggaggag gtcgtcgagg aggctccggg cctgggtgaa gttgccggcg agggcggtct
       61 tcatcaggtc ccggatctgc tcggggcggg cggtggcggt gatctggtag atcgtctcct
      121 ccgtcacctc ctcctcctcc atcagggagg cggcctggag ggcgttgatc gccttcctca
      181 tgtcacccct ggcgacgtag actatggccc tcatcccctc cggggtcacc ttcaccccct
      241 ccgcctcggc gatcgtcttc atcctctcgg agaccgcctc ctcggagagg gacctgaacc
      301 ggtagacggc gcaccgcgac tggatcggct cgatgatctt ggnnttggag gagtagttgc
      361 aggagaggac gaacctgcag atggagctgt acctctccat cgtccgccgg agggcggact
      421 gggcgtcgtt cgtcagggcg tcggcctcgt cgaggaagat caccttgaac tcggcgtttc
      481 cgaggggggc gatccgggcg aagtccttga ccttgtggcg gacgacgtcg atcccccgct
      541 cgtcgctggc gttaagctcg atgaagtttc cccgccactc ctctccgaag agctccctca
      601 ccatgctgat ggcggcggcg gtcttgccga cgccgggggg gcccgagaag aggaggtggg
      661 ggacgtttct gctcctgacg taggacttga gcctcttcac gatctcctcc tggcccacca
      721 cgtcgtccaa gctcatcggc ctgtacttct cgatccagat ctcctctttt atcctgtacc
      781 cctccttcct gacgacctgg cccctatctc tatatctaat ttcccacctg ccgccggggt
      841 gcgatcggtg cagagcccga tctcgccgat ggggggaggg cggtccgggg ccgccgtata
      901 cattcaaaaa tttatatata cgaagcccgc cgtagtattg agagaggtgg atctgatggc
      961 ggattttatg atggcatcta tgaccctgat cgtcgccctc ctggcggcgg cggccctggc
     1021 ggccccggcg gcggggatcg ccgatccggc ctgcgtctac tgtgaggccc tcggctacaa
     1081 ttgtagcaac ggaaactgca ccttccccga cggatcctcc gtcccgacct gggagttcta
     1141 ccgcgggaag gcgggccaga agtattcgtt ctgcgagctt caaggatatc ggatcgagag
     1201 ccggaccgag gatatgggcg gctggaccag cgagtacgcc gtctgcgtct tcgatgactg
     1261 ctccgagtgc ggcgaggagg agcacttcga cggcacttgc ggcccttcca actgcagctc
     1321 ctggaccatg gccgaagggt gccggccgcc ctttgaactc aaaggcctga tctcgatggc
     1381 agcccggatc aacaactctg tcggccgggc cgcggaggac gtcctcggct gggacgtggt
     1441 ataccagggg gaggatggga tctgccgcag ctactacctg gcccaggagc ctctgatcgg
     1501 gatgaccgag ccggcggagg tggactgtcc ctcaggcctc aaacccttcg actggtacat
     1561 ggccggctat gaggaggcga tcgccagcat gaggtcgatg cgttgcggcg ccgccttcgt
     1621 gaacctcacc ctcagctggc cggcggcccc ggagttcggg gagcctgtct ggcggatcac
     1681 caccgacatc ggaaacgaga tcgtcgtcgg cgcaaactgc ggcctcagcg gatgcaggcc
     1741 ggccgagcag aggacccctt gaggagagaa tgttcgggag agggggaggg gctggaggag
     1801 atagaggccg aaaatcttcc cgtcctctgc tccttctgat cttctcatat tgaccggctc
     1861 gaataggtgg acctatttaa acacttagaa gaaactaaaa gcaggtgctc tggataatta
     1921 tccatggctg aaaagccgca ttcagccgat ccagcacctc tttgggagat tcggcccgtc
     1981 tcttctcatc ttccgtcctg cagtttcgga tcttgccggg aagagtgagc tatcggccta
     2041 gtcagatctc ctcccccagt tggatccgga tgtgcttctc ctccatgtac tcgtataagg
     2101 cgcaccggga ccgctccctt cccaagccgc tctccttcca gccgccgaag ggggccccgg
     2161 ggactatggg gcttgtggta ttgaccccga cggaccccgc ctccagcctt tcggcgaaaa
     2221 agagccccct cttcatatcc ccggtgaaga cgtaggcggc aaggccgtac cgggaggcgt
     2281 tggcgagcct cagggcctcc tcgtcggtcg aaaagctnng cgccacgggg ccgaaggtct
     2341 cctctctcat catcctcatg gaggggtcca tccgatcgag gacggtggga aggaagaaga
     2401 agccgcggcg gaacttttcc ccctccggcc tcctccctcc ccagaggacc ctcgcccccc
     2461 gctccagggc gtcggctatg tggtcctccg tcttctttcg ctgcgcctcg ctgaagatgg
     2521 gcccgaggtc gacattgggg tcgaggccgt ctccgatccg gagcctctcc gtccgctcga
     2581 cgaccttccc ggcgaactcc tcggcgaccg cctcctggac gtagatccga ttgacgctga
     2641 tgcagatctg ccccatgttc gagaaagccc gctggacgca ggccgccgcc gctccatcca
     2701 ggtcggcgtc gtcgcagact atggcggggg cgtgcccccc cagctcgagg gagagccgct
     2761 tgacggtctt cgccgccttc gccatgatct cttttcccgt atccgtctcc ccggtgaagg
     2821 ataccttccg ggggatgggg ctctccacca gggccccgcc cacctcctgc cccgagccgt
     2881 ggacgaggtt gatgaccccc gccgggagcc ccgctccggc gacggccatg acaaacctgg
     2941 tggcggcgag gggagcctgg ctggagggct tggcgacgac ggtgcagccg gcagccaagg
     3001 cgggggcaac cttccaggcg aggaggtcca ccggatagtt ccagggggag atgacggcgg
     3061 cgacccccaa gggctgcctt atgacgaggc tccggcttct ccccgaggac gggacgacct
     3121 ccccggtgat cctctccccc tcctcggcgt agtactccag ggcctccgcc gatccggcga
     3181 cctcccgcct cgcgtacgcc aggggctttc cctgctccat cgtcagcaga agggctatct
     3241 cctcgatcac ctcccggact gcctccgccc cccggcggag gagcccaccc cggaccctcg
     3301 gctctctttc cgcccaggcg ggaaaggctc tcgccgccgc ctccagggcg agcctggcct
     3361 ccgaacgacc gccgagggcg acctcggcca ccgcctcccc cgtggcgggg ttagttatca
     3421 gagcccccct cccggtggcg gagtccacca cctctccgtc gatcagcatc ttgaaccgtt
     3481 ccatcagatc caactccagt ttgcgatctc agcttcaaga cctcgattca gacctctaat
     3541 atgcccctct cccgagataa agggggagga tctctccccc tcaggggtgc ctcctcgtcc
     3601 ccatcgaccg acaccgtaaa attctcccnn ccgaggcgaa ccgagttgat aagagataga
     3661 tccatcctgg tgacgggggg cgccggcttc atcggaagcc acctcgtcga tcggctgatc
     3721 gattcggaca gggtcaccgt cctcgacaac ttcagctccg gaaaggcgga gcacctcgag
     3781 gaccatttaa aaaaccccaa cttcaccctg gtggaggggg acctcctcga ccggaaggcc
     3841 gtcgaggcgg ccctggaggg ctgcgatctc gtctttcacc tggccgcaaa ccccgacgtc
     3901 aaggtcgggg ccgaagacac caaaacccac ctccaccaga acgtcatcgc cacctacaac
     3961 ctcctcgaat cgatgaggct tcgggacgtc gccgagatcg ccttcacctc cacctccacc
     4021 gtctacggcg aggctgggat cgtcccgacc ccggaggggt acggccccct cctccccata
     4081 tccctatacg gggcgtcgaa gctcgcctgc gaggccctca tctccgcctt ctgcggcacc
     4141 ttcggctttc gttcctggat cttcaggttc gccaacatcg tcggggaccg ggggacccac
     4201 ggcgtcatcg tagacttcat aaataagctg acggagaacc cggaggagct tctgatcctc
     4261 ggctccggaa ggcagaggaa gtcgtacctc ctcgtcgacg actgcgtcga ggcgatggtc
     4321 tttgccatag ccagagccgg cggaagggtg aacgtcttca acgtcggatc ctccgactcg
     4381 gtggacgtaa ccgagatcgc cgacgtcgtc gtcgagaaga tgaacctctc aggggtgagg
     4441 tacaggtaca ccggcgggat cgaggggcgc ggctggcggg gggacgtcag gacgatgctc
     4501 ctctccatcg aggctctgga ggagctcggc tggcggccga aattcagctc cagagagtcg
     4561 attgagcggg cggcggaggc gctcctccgc ccgagatgag cccccccgag ccatcccgcc
     4621 gccctgacca cgggttcgcc tccgggagga tggggcagaa tatgggggat agaaggcgct
     4681 ggagacagga gggatccggg cggaggggga gagccgaaga taggaagcat tgggtagtcg
     4741 gacggcgaag ggtgagattc gccgccggtc cgaatccttc aggggaggac cctgagcctg
     4801 agcttagaga agtcttcctt cacctccacc ctgatcccca tcttggagaa gatgtccaac
     4861 agctccgtct tgatgaactt tttcggtatg tcgtagccga ggaggagggt gaagtccgtc
     4921 tccgaggtcc tgctcagctt gaagaggttg cagagctcaa gcctcctcaa gaccgcctca
     4981 gccttgtggt tcttcttctt gaactcctcg gcgtgggcct cggagacctg ctggtgcatc
     5041 tcccagaacc gctccctatc ggggtgggtc tcgatgaagc tgagaaagag gatccagtgg
     5101 tcgatgtcga ggatgatgtg ctcgccgacg gatagaaggt cggagtaggc gtagatcttc
     5161 tcctcgtcga cggagctgaa gagaggcttg tgcttgctgt agaacttgag ggcctcccgc
     5221 atcagctcgc tctgggatat ccccagatct tccctcatct ctcggaagag cttcgcggtc
     5281 tcgtcgtcta gggctatcgt gaccctctcc ggggcttttg ccatatcgag cctcctttcc
     5341 cccgcatgta tttacctttt tttccaatcc ggctcctccg acggcgccga tattccgaga
     5401 ccttacctcg gggctacagg gagggatctc ttgggaaggg cgcggctggg ggggaggtca
     5461 gcctggacct cgctcccagc tgaagagctc ttcgtccgac cttttggaga tcttcagggc
     5521 ggcctcctcc agggaggcgt acctctccct ccagctcttt atctcaccga cgacctcgtc
     5581 catcgccctg atagtcaccc tcctcccgga agcggagaac tccgcctcgg cgatcccggg
     5641 gggctcgacg gatatgttgc ccctccccag ggtgcagccc gccgagacct ggatcccgtc
     5701 caggaggc
//
LOCUS       DASM01000064            4016 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_282810, whole genome shotgun sequence.
ACCESSION   DASM01000064 DASM01000000
VERSION     DASM01000064.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_282810"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    139..268
                     /estimated_length=130
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          817 a         1276 c         1161 g          632 t
ORIGIN      
        1 tcaaccggat gaacgacctc gtcgacatcg tccggatcga gggagagatc ctctatgacg
       61 gcgtcaacat ctacgacaaa gatacagacg tggtggagct tcgaaagaac atcgggatgg
      121 tattccagaa gccgaaccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg ctctgcatcg cgagggccct ggcgatgaag
      301 ccggacgtca tcctcttcga cgagccctgc agcgccctgg accccctctc gacggcgaag
      361 atcgaggagc tgatggacga gctgaggggg tcttacaccc aggtgatagt gacccacaac
      421 atgcagcagg cagcccgggt ctcggactac accgcctact tcctcctcgg cgagctggtc
      481 gagttcgggg agacgaagga gatcttcgag aaccccagcc agaagagcac agaggattac
      541 ataacggggc ggttcggatg agttcaccga aggaggtggg ggagagatga gcccttacag
      601 agagcgttat cataaggagc tggaggagct gaaggcggac gcggagaagc tggtggacct
      661 cgtcggcttc gcagtggcca agtcggtgga tgccctccgc gactacgacg tggagaaggc
      721 gcgggaggtc ataagggacg atcagacgat caacgacctg aacctgagga tagaaaagag
      781 gtgcatgcag ctcttggccc tccagcagcc gatggcgaaa gacctccggc tgatcgtcac
      841 caccctgaag atagggatcg acctcgagcg gatcggcgac ctggcggtgg acatagccag
      901 gatcgtcgtc cactccaaga acaaggtcca cgtcaagagc ctgaagaaca tccccaggat
      961 ggcggagatc gcggtggaga tgctggccca ggcgaacagg gccttcaagg agaacgactc
     1021 gaacctcgcc cgggagacga ccaagtggga ttacgaggtg gacgccctct accagaaggt
     1081 tagggacacc ctcctcaaga tcatcggcgg aaaccccgag ctgatcgagg acgctacgcc
     1141 cctcctcctg gtcaacaagc acctggagag gatcgcagac cacgtatgca acatatgcga
     1201 gagcatcata tacatggttg aggcgaaacg agaacatctc aactgatcta gcaggttttt
     1261 atggagacca ggaaagtaca gaggacggga aagtcgacct tcatcgtctc cctccccaag
     1321 ctctgggcgg tgaagaacga tatccagcca ggctccttga tctacatcac ccagagcgac
     1381 aacggcgccc tggtcctctc ccccgacagg tctgagaggg agctggccac caggctggag
     1441 atcggcgaca aggtcggcga cccgctgata agggacatca taggctgcta cgtctccggg
     1501 tacaggacga tggaggtcac gggagcccac atgacggcgg accagaagag ggacatccac
     1561 cagatcgtcc agaagctgat cggccccgag atcctggagg agacagtgaa caaggtcctg
     1621 atccaggacc tgatcgatcc ggaggagctc cagtcggagc gggccctccg gaggatcaag
     1681 actgtggtga ggtcgatgat ccaggacgcc ctgagcgcca tggtgaccaa cgaccgggag
     1741 atggccgccg acgtcatcca gagggacgac gacgtcgatc gtctctacct gctcgtctcc
     1801 cgccagttca cggagatcct caggtccggc tctctgaagg aggagtccat cgaccccatc
     1861 accgccttca actacacccg agctgcgact aacctggagc ggatggcgga ccacgcctcc
     1921 aggatcgcgg atatgatcct gcggtgcgac tgcaccctcc ccgccgaggt cgccgagggg
     1981 atgggccgca tatcccccca gctcctgagc ctcatcgagg actccatatc ctccctcctc
     2041 ctcctcaact ccggaaaggc gaacgaggtg atagagctga gccggcagct gaggagggag
     2101 atgatattga tggtctcctc cgccagctcc tccggggagc acgaggacgc cctcctgagg
     2161 atggtggcct cgagcatcga gcggatcctc gactacatca agaacatcgg cgagctggcc
     2221 atcaacctca gccagacagg accccgcccc gaggcgaagt agaggtggat ctcccgcccc
     2281 cctcttccgc ctggagaggc tcctcgcgca cccctggagg accagtcctc tacggatctc
     2341 gtgaaaaaat gaccccgccg gggggggacc aggccttcat ctccggtccg agggagctgg
     2401 tggtcagcag cgccgtcacc ctcgaccccc ttccttccag atcgcctcca gatcctcggc
     2461 ctcctcacca tccgggaagg gccgcctgcc ctggaggcgg tgaggaggct tgatccatcc
     2521 cccaccctcc tcttcgtcga cagatgcggg atggaccacc cccggcgggc ggagatggcg
     2581 agggccatcg gcgtcgccct aaacctcccc cgccggcgcc tccgggacta gaagcttcct
     2641 cggccgtgcc tcgcccccca tagagatgca aacgacgaaa agcgacgcat tcagatgggc
     2701 gacgccccca catcatcccg gtcgactatt tttcacccga aaaaatgacc ctatcccgga
     2761 gaggtccccc tctcgacctc cagaatcctc cgcttgaggt cgaccccggg gttgtagcct
     2821 cctagatccc tcgaccccac cacccggtgg cagggtatga tgagggggat ggggtttctc
     2881 ctcagggccc cccccaccgc cctggcggcg ccgggccggc cgagcctctc ggcgacttcg
     2941 ccgtaggtcg ccgtctcccc ggggcggata gccgttgtgg cgaggaggac ctccctctga
     3001 aagggcgtca gccccgagag gtccagctcg aaaggcaaag gctcgccgcc gagccagcac
     3061 ctcaggaggg cccctttcca ccaggccggc aggagatcat cgggcggctc cgtggagagg
     3121 cgtgccccag agatccggtc gtccctcctc tccaccagga ggtaaaggcc caggggctcc
     3181 agaaagagcc cctccgtcgg accgggatcc tccgccacct tcacccgccc cggggccgcc
     3241 ttcccgtcat atccctgatg gaggggcgag atcccatctc caccacctcc acccccatcg
     3301 actccgtatc caggatcgcg accgtcttcc tcccggtgaa gtggccccag acctcccccg
     3361 ggttgatcat cagcgtcttc cccggcacca cccggaggtg atggtcgtgc ccccggacga
     3421 cgacgtcgaa ggcccccgac tccaccagcc ccgtcaccag gggctcgtag gtcccgtgga
     3481 ggagggcgat ccgtcggccg tcggcctcca cctcgccgaa gtcctcgagg aagacccccc
     3541 cgacccggcc gaagtcgcgc ttgaggccgg ttcgatctcc gtcgttgttc ccgtagacga
     3601 gctttagctt ccccgcccac ccctcgaagg cgtagcacat cccaggagcc accacgtcgc
     3661 cggcatgaag gatcatctcg acgccccgtc tcctgaagat ctccaccgcc tccaggaggc
     3721 ctggaaagtt gtcgtgagag tcggacatta gaccaatcag catgataatc acaaaccttc
     3781 tgccggagcc cgggccttag ggctctcggc cctgctggcc atgggaaggc cccgatcctc
     3841 atttatagct tttgacgcca taatttcaga aacggatatg cgtcacgcca gtatgatccg
     3901 aaagggctat atacgagaat ggctttgcat cgttggaccc gccttaactc agcctggtag
     3961 agtgcgcggc tgtagtaggt tatgcaccgg atggtgcacc gtgcggctac cgcgat
//
LOCUS       DASM01000065           16441 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_39802, whole genome shotgun sequence.
ACCESSION   DASM01000065 DASM01000000
VERSION     DASM01000065.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 16441)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16441)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16441
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_39802"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2716 a         5413 c         5247 g         3065 t
ORIGIN      
        1 gtctccggca tcgacgtatt ccccagggtc gaggaggggg acaggatcgt cctccggcag
       61 ggggagggtt gagccggagc ctcgccctcg ccgctgcggc gcgttctcat gatcagagga
      121 agattagagg aagattaata gtgaagacgg cggatacatt ctgagcagtt caatgcagga
      181 tgatctgacc aggatggtct tagcaggatc ttgaggtgaa cgagatgacg aaggtattgg
      241 tagtctacca ttctcggggc gggaatgtcg ccaggatggc ggcggcggtc gccgagggcg
      301 tgaaggaagg aggggcggat gtggtaatga agaaggtcga agaggcgacc ttggaggacc
      361 tgaccggagc cgacgggatc gtcttcgggg cccccaccta ctttgggacc ctctccgccg
      421 agatgaaggc cttcatcgac gggtcggtcc gggtccgggg ccgcctcgag gataaaatcg
      481 gcgcggcctt cacctcctcg gggagcccca ccggcggaaa cgagacgacc ctcctgtcga
      541 tgatccaggg gatgctgatc cacgggatgg tgatcgtcgg cgaccccctg gagaccggcg
      601 gccactacgg agccgtcgcc gtgggggccc ccgacgcctc ggccctcatc gcctgccgga
      661 agctcggcga gcgggtggcg aagctggcga aatccatcgc ctcggcctga tgggaagtcg
      721 cgagatgacc cgacccatgg atcagctgct ggatacggcc gagaagatca ggtcgatgga
      781 gatccggggg gcggccctca tcgggagggc cgcggccggg gccctgaagg agttcgccct
      841 cgccatcgat ccggccggga tcgaggagtt caacgcccag gtggacaggg ctgccgacat
      901 tctgctcaat accaggccca cggcggtgtc gctatccaac gccatcagga tggtgatgcg
      961 ctaccgcggg gatgatctgg agtcggcccg aacctccctg gcgaagaacg ccgacggctt
     1021 cgtcgagcgg tccctgatgg ccgtcgagag gatcggggag atcgggtccc ggagggtccg
     1081 ggacggcgac gtgatcctca cccactgcaa ctcctcggtg gcgctatcca tcatcaagac
     1141 cgccttcgac gccggcaagg acgtcaaagt catcgccacc gagtcccggc cgaggtacca
     1201 gggccacctg acggtcaaga ccctggacgg ctgggggatc gagacggagc tggtcgtcga
     1261 ctcggcggtg aggtcggtga tcaacgaggt ggacgaggtg atcgtcggcg ccgacgttat
     1321 caccgccagc ggctctctcg tcaacaagat cgggaccgcc gccatcgccc tcgccgctca
     1381 cgaggcgagg acgagcttca tggtggcggc ggagacctac aagttcagcc ccgagacgct
     1441 gatgggggag ctcgtcccca tcgaggagag ggcggccgag gaggtctacc ccggcatatc
     1501 tgagcttccg cacgtccggg tgaggaaccc cgccttcgac gtcacccccc accagtacat
     1561 cgacgtcatc tgcaccgagg tgggggcgat cccgccggag atgagctacc tcgtcatcaa
     1621 ggagatgctc ggctgggaga tggaggggcg ggtcccctga ccccctctcc tccctcctca
     1681 cctggggatc cctaagaaga ccgtctcagc ctctcctccc ccgccctctg aggtggaggg
     1741 ccaccgccat cgcccccagg gcggccggga ccccgagccc cggcatcccc tccgcctcct
     1801 ggggtttttc ggtaggtgcc tccccctcct cccggacgat ggggcaggcc ggatactcct
     1861 cggggagttc gtccctctca ccttccgtcc tggggacccc ggcctcggag acgatcactt
     1921 ggacgggggt ccactccggg ggggcgcccg ccgccacgat ccccacctcg aaggagtagt
     1981 tccccggcgg gacgtccccc gcctctaccc tcagctggca gtcgaactcc cgcctcgtcc
     2041 cggagtcgac ccacttggtg caccggggac cgacgatcga tagcccctcg gggatgttcc
     2101 tgaagatcag ccccgcatag ccgctctggc tccccagatt ctggatgaag acggtgaact
     2161 ccgcatcttc cccggggctt acccggacgg cggggacctc gactatcttg aagaagaccg
     2221 acgcctccgc ctctccggcg atgaggagga ggagaagggc agcaaaagct cctttcatgc
     2281 tcttttatcc gctgaccatg attaagctcc tttcgggcgg atatgagaag gagagggcgg
     2341 gacctgaagg acaaaaaatt gtggagatct tcggcctccc tgccgggagg cggtagaccc
     2401 ccttgaatct tcctaagccg gacctaaacc ggcaaaggcg cgccggctcc gacggcgaac
     2461 ctgtccctga aggtggtcac gtccacgggc tttataccgc cgctggcgtt cgtatcctga
     2521 gcagggggtg ccgccgccgg gatctccggg gccggggcca cggcggctgc tgcgggagca
     2581 acggcggccg ggacgttggc aggctcatac cccgaggtgt cggggctcag cagaaatcct
     2641 gtgacgatcc cctggctgac cctcccctgg ctgtcggact ggacgaagct ccccgagagg
     2701 tcgtcggcgg tggcggaccc gacgatcacc gtcgagacga ggactccgtc ctggagggag
     2761 aggatctgca cctcaaatcc cccttcggag gcctcccccg tgaccacgcc gttccagggc
     2821 ttcggggcct cggagttggc ggcgccgtag aggagcccct ctctctgatg caccatcatc
     2881 gaggtctgac cgtcggccat gttcaggatc caggaccctt cgaggctctg gaccagagcg
     2941 gctgcatcct ccggggccgg gatctcggcc accggcgcca tctcgacggc ttccgccgtc
     3001 gctgcgacgg agtcgtttat gctatcgttc atcgggatcg gctcttcttc tgccattgag
     3061 agtcccgata tgagggtgat ggtgatcagc gctgatagag cgatccatct caggttcaat
     3121 ttaacacctc tgacatttta tcccactccg ttgatgatcg gcagctcttt caacccgctg
     3181 ccgaagggat gatctcgttc caccattaat aagcttttca tagaccgaaa gcccggattt
     3241 tgcccctcag gagctcccag cggcctcctc caggagcttg agcaccgccg ccgggtcggt
     3301 cgtcggctgc ctgcagaccc tccccgagca gacgtaggcc gtcgtcctcc cctcctgagc
     3361 ctccatctct gccgtgaagc cggcgagctc gacgatcctc cggtctttcc ccaccggcct
     3421 cccgaggacc accttcctcg ggaggaacct cgacctgagg gccttcagca tctccgccgt
     3481 ctccggggac ccggcctccc ccaccaccac caccacctcc gaggacggcc ccagggcgaa
     3541 gtccagggcg gatagcaggt ggaggaacgc cgaggggctc cccctcacct gggcggcgaa
     3601 ggcctggatc gcctccgatc cgatctcctc cagctccggg tcgccggtca tcctggcgag
     3661 cctcaaaagg tcgaaggcgg ccacggcgtt cccggcaggg agggcgccgt cgtggccgtc
     3721 cttcctccgg acgatcagct cctcgccgtc ctccggggtg aagaagaagc ctccaccatc
     3781 ccggtcccga aacctctcca ccatatctcg ggcgagggcg agggcggcct ccagccgctc
     3841 cggccggaag cccgcctcgt agagctcgag gagcccccag accatgttgg cgtagtcgtc
     3901 gaggttcccc aggatcccgg cgtcgccgcc ccggtaccgg tggaggagcc tccccctctc
     3961 gtccctcatc cgatggagga cgaggtcggc ggctcgatcg gccgcctcct ccagacggcg
     4021 atcccccagg acctgggcgc cccgggcgag ggcggcgatg gcgaggccgt tccagtcggc
     4081 gaggatcttg tcgtccttct tcggcctcgc cctcccctcc cgggcggcga agagcttctc
     4141 tcgggccgcc tccagcctct cccgaaggtc ccccaccggg atccccagct cccgggcctg
     4201 gtcttccagg gggatcttca tggcgaggac gttctttccc gggagcctcc ccgtcgcctc
     4261 ctcccggaag ttcccctcct cccggaggct gaagatccgg gccgccagct ccgcctcgtc
     4321 ggggtcgagg acggcccgga cctccggcag ggtccagagg tagaacttcc cctcctcccc
     4381 ctcgctgtcg gcgtcctcgg cggagtagaa gcccccctcg ggggcggcga ggtcccggag
     4441 gaggtagtcg aagacctccc gggcgatcgt ggcgtactcg gcctttcccg tcgcctgatg
     4501 ggcctccagg caggcgaggg atatcatcgc ctggtcgtag agcatcttct cgaagtgagg
     4561 taggtgccag cgcagatcgg tggagtagcg gtgaaaaccg ccgcccaggt ggtcgaagat
     4621 cccccccctc ctcatggcgc ggagggtgac ctccgtcatc tggacccccc cggggtcgcc
     4681 ggtcctcctc cagtggcgga ggaggaagag gaaggtcgtc ggcgacggga actttggggc
     4741 tcccccaaag ccgccgttgg cggcgtcgaa gcgggcgacg agcccctgat aagccgcctt
     4801 tatcgtcgcc tcctcgaggc ggagccccgg cacctccggc gggggtcggc ggaggacgga
     4861 ggccacctcc tccgccgagg cgaggagcat agcccgctcg ttcttccaga ggtggccgat
     4921 ccgggggatg aggtcgagga tcccgatcct cccgaacttc gactccttcg ggatgtaggt
     4981 ggcggcgaag aagggctttt tgtcgggggt gaggatgacg gtgaggggcc agccgccgga
     5041 gccggtcatc atctgcgcca cggccatgta gacggcgtcc aggtccggcc tctcctcccg
     5101 gtcgaccttg atggggacga agctggcgtt caggagcctc gccacctcct cgtcctcgaa
     5161 ggattcggcg gccatgacgt ggcaccagtg gcaggtggag tagccgatcg ataggaatac
     5221 gggcttatcc tccctctccg ccctggtgaa ggcctcctcc ccccaggggc gccagtcgac
     5281 ggggttctcg gcgtgctcca ggaggtaggg gctcttctca aaggccagcc ggttcttctt
     5341 cttcacctta tcccctcccc cccctctcgg ggcgtctcct ccgggatgac cgccccttcg
     5401 gggacccctt ttctcctcaa agctccgcca ctgaaagcgt cggcggggtc gcaccctccc
     5461 gcctcctcat ccccctcgtc ggcttcttca accttcccgg cctggacgta cccccagagg
     5521 acctcccccg ggcgtcccca ccgccccgcc tcgatcaccg tcccggggta tacgatcgtc
     5581 cccgccccgg tcttcacccc ctcgccgagg acggcgccga gggccttccg gccggtctcg
     5641 gttccctccc cgccgggata ggatcggacc ggcgagccgt cgtggcgccg gttggtgatg
     5701 accgtcccga ccccgaggcg gcacccgggg ccgatgaggc tgtctccgac gtagcagagg
     5761 tgggatacct ccgccccctc catgacggtg ctcccctcga tgacggtgcc gcttccgatc
     5821 cgcaccccgt ctcctatcga tgtcgcccct cggacgaggc agttgggccc gatctcgcaa
     5881 ccttccccga tcaccaccgg ccccgccacg taggagccgg acctgatcgc cgtccccctg
     5941 ccgacggcca ccttcccgga gagggaggcg ttcgcctcca cctcccccag gaccccccgg
     6001 gtctcgggag gcatgagggt ctcgttcgcc ctcaggatgt cccaggggaa gaggatctcg
     6061 atccacctct ccaccacctc caccccgatc tggaccccct cggcgaggag gaccctgacg
     6121 gcctccaaaa ggtccctctc ctccccaccg ggggggaccc ttcggagggc ttcgaaggcc
     6181 cttttatcga ggaggaagat cccggcagag acgaggttag aggggggatt ttttcctcct
     6241 ttggagatcg attttaggag gccctcgctc acctccaggg tcccctcctc caccggccgg
     6301 gggccttcta ccctcgccgc cgccatggcc ggcgcctcca gggccatcag cctcctgacg
     6361 gagtcggcgt cggggaggac gtcgcctcgg aggacgagga accgatcccc ggctagggcc
     6421 tcggccgcca ggagggcggg gccggccccg acccccccct cctggaaggc gtactcgatc
     6481 tcgacctcga ggcggctccc gtccccgaag gcgccggcga tcttctcccc ctggtggccg
     6541 acgacgaaga cgaacctctc cgcccccgcc tccttcgcca aaaggagaac gtgctccagg
     6601 aggggctttc cgccgatggg aagcatcccc ttcgggaggt tggcggtgag ggggcgcatc
     6661 ctcctctcct cccccgccgc gaggattatc gccttcacga ggatcccgcc tccctcaatc
     6721 tcccctgaat atcctcagcg cctcttcgac ggtggcgtcg tcgagggtga tggcgctgat
     6781 ggcgttgcag atcctcaccg cttcatcgag ccccttctgg tggatgttcc ttccggtggc
     6841 gttcccggcg gcgccgctga cgtggatctg gtcgaatagc tcctggagga acgacttcgg
     6901 gtcgcggctg gctcccccgg cgcagacgac cttagtcctc ccggcggcgg ccaccgcctc
     6961 cttgaagatc aactcggagc tcgccccctc cctcttcgga tagttgacct ttacgaagtc
     7021 cgaccccagg gcggcggcga cgccggtcgc ccccgcgatc aggtgggggt cccgttcgtc
     7081 tctgacggct cttccccggg ggtagatcca gaggacggcg acgaggccgc ggcggtgggc
     7141 ctggaggacg atctgggaag cctcccggag catctttgcc tcatactcgc tcccgagata
     7201 gacggtatag ccgaccccca ggacttcgag gccgctatcc ttcgcgaact cctccacctg
     7261 ggagacctcc accagctggc ggctgagggg gtccctctgg tcggcctgga cgaggtcggt
     7321 cttcgagttg agcttgacga ggtacgggac cctcgggtag tccatcccgt agcgggcgat
     7381 gaggccgagc tgggcggcga agaccccgat cctcccgcgg tcggcgatcc tgaagaggtg
     7441 ctcggggtct ccgtcctcgg ggctgatccc cgggccgctg aagtcccggt tgaggtgctc
     7501 gaccttctgg tctccggcga agagcatcag ccggccgctc cctctcgtga tcttcaggta
     7561 gctctcctgg tagtcccacc gccttccggg cggaacgtcc gcggggaccc gcacctcgtc
     7621 tcgttccatt cttctcgtca tcttcctcaa tcctccgata tcgtctcgag cctcacctgg
     7681 gggacgatcc tctccacgtc ctcccgggtg gcgagggcgg tcccctgccg gagggtcgtc
     7741 gccgtcccgg cggccgtcgc ccagatcagg gactctttca ggtcaaatcc tttcgagagg
     7801 ccgtagatga aacccgccac cgaggagtct ccggcgccga tggtgttcac cacctccacc
     7861 tgggggggga ccgcctggta cgccttcccc tcggagacga ggaggatccc cctcggcccc
     7921 atcgatacca ggacgattcc gacgccccgt cggtttatct cccgggccgc ctccaggatc
     7981 tcccccctct tctccagcct cttgcccacc agggcggaga gctcgtggat gttcggcttt
     8041 atgacgtcgg gcttgcccct tatccccacc ctcagagctt cccggtccac gtccaggacg
     8101 accttcgccc tccggagctt cgccgtctcg atgatcttcc gatagacttc ggggtgggcc
     8161 cggggcggga ggctccctcc gatggagacg aactccggct cctctatctt cctgaccagg
     8221 tcggagatct cgaggagctc gaagggcttc acctcggggc cggcggagtt gaggatgatc
     8281 tgccttccgc tcccaagctc gtggatgacg acgttcgtcc tcgtctcccc ggagacgggg
     8341 atgaagctgc agtcggtccc gtcccgggtc agctgagcct ccagctggcg gcccgcaaac
     8401 ccggcgatga accccagggc ccggttcggg acgccgaggg cggtcaggac cttcgatacg
     8461 tcgatcccct tccctccggg gtagctctcc tcccggatga tccggttcga tacatcctcc
     8521 ttgatctcgc tgacccagag ggtcctgtcc agggcggggt tcagggttat ggtgtagatc
     8581 acagattaca tctccgttta tggattgggg ggccagctct ccagccaccc ttcggggtct
     8641 gttggagcgg ggtcaccagc ctcgcctctt ccggtagatc ctcatcatct cctcgatcgt
     8701 ctccggatcg atcatcccga ccgcggagac cgtctcgtag aaccgtcggg gtatccgggc
     8761 ctctgccagg tcgaacccct cccgcctctt gaacctgtac ttctcgtcga agatcctccg
     8821 gccgagctcc tccagctccc cttccgtctt ctcgatcccc accgagccca gggcctcgag
     8881 gatgttcttg ggggtgtaga cccccctcgc gaagaggcag ccgacgaggg agttgtagac
     8941 cccccgggag tcgtcctccc ggatgatggc gtcgaccatc tcctcggggg tgaggctctt
     9001 cttcagagct cgctggtcga tgctgtagcc ggcgttgtcg aggtgggagt gtctcacccc
     9061 cacggtgagg ccgactactg aggcggggcc ggtgtggtag ccggggacct cgttcttccc
     9121 cagggccatg gcgaactccc cgccgccgta cctctccgag gcgaagacga ccccttgggc
     9181 gagggcggcg tagaactcgt tctccatcct gattatattc tctatcgcct tcaggtagct
     9241 ggggacgtcg ttccaccgga gggatacccc cagggtctcc tggagggtga cgagcccctg
     9301 atccagggcc tccgtcgccc aggcgaggac gaccccggtg ctgatggcgt cgagcccctg
     9361 ccgctcacac gcctcgatca gctccagcac cccctccgcg gaggagaccc cgaggttcga
     9421 ccccagggcg tagaggagct cgaagtcgta ggagatcttc ctgatctcga actcatgctc
     9481 gcgagagaag gaggtcttga gcatcccaat gtggatgcag ccgatggggc agtgggcgca
     9541 ggagaccctc cggaagaggt agttgtcggc gaggacttcg ccgctgatct tctcggcccc
     9601 ctcgaagctg ctatcctgga agttccgggt ggggagcccc ttcagctggt ttaaaacgtt
     9661 gatgttgatg ggggtgccga tgtcgtggta cttctccatg gcgtcggtct ggacgacggt
     9721 cttgaatagg cggttgtaga tccgcctgta cctcctgggg tccgggacca tcacgtcctc
     9781 ggtccccgag acgacgatcg ccttcagctt cttcgacccg aagacggcgc cgaggccgag
     9841 cctcccgaag tggcggtagg tgtcgacgac caccccggcg tacctgacga gcctctcccc
     9901 gccgggtccg atcctgatta tggaccttcg gccaaccccc gtctcgacgt cgcggaggat
     9961 gtatccgacg acggtggcgg ggacccccca tatcgaggtg gcgtccttga tcttcacctc
    10021 gtcgttctcg atggatatgt agacgggccg ctccgccgcg ccccttacta cgaccgcctc
    10081 gtgccccgcg aacctgaggg ccatcccgag cctcccgccg gcgtaggact cgccgagctc
    10141 gccgctcaag ggggacttga aggcggcgac ggtcttggtg atggccggaa agagggcgtt
    10201 caaggggccg atgctgaata ctatgggggc ctccggggag agggggtccg ctcccggggg
    10261 gcactcctcc aggaggagct gggtcgccac ccccgtaccg ccgagccact cttcgaagag
    10321 atcctccctc tcctcgacgg cgctctcgcc cctccccagg tctatgtata gaacccttct
    10381 cagcatccta caaacctccc ccccattcct ccacctcttc catctccagg actccgtggg
    10441 ggcagaaggc gacgcaagcg ccacagtgct tgcagcagac cgtctgcccc tcgtcgtcct
    10501 gggatatggc cctgatgagg caggcttcgg cgcaggcgcc gcacccctga catagctccc
    10561 tattcaggag gaccccgccc ccatccctcc ggacgagggc ccccgtgggg caggccctgg
    10621 cgcagggggg gtccgtgcag gcgcggcaga cgacgacggc gaagtcccct tcgagccccc
    10681 cctgggtctt gactttgatg gcgctccggt ccggggacgc ccggccggcg ttgatcctgc
    10741 tgcaggcgaa catgcaggag aggcatccta tgcacctctc cgggtgggcg accttcagcc
    10801 tccgcttctt cctcttcttc cccccatccc cggccccccc cgccagcgct ggggcccccg
    10861 tctcttcggg ttctgtcgac gatcccaatc ctgcgaaccc cctcgatctt gatcggttaa
    10921 agtcccgccc tcttcctgga ggtgatctga cccccgccga cgagaacgac ttaaaaatta
    10981 gctcgcataa ttattaacca tttctcgtcc aggccccgga ttggggatgg agggtggagg
    11041 gtgcggctct ggggggagtt ccgggccctt cgatgggggg caggtcggag gggagggggc
    11101 ggcccgccct ttcccgcctc aatcggcgtc cgcgaatctc tcccccaggt tcttgaggac
    11161 gtgggggagg gtggtgtact ccatatcctc catcggcagg cggtggggct cgaaggggcc
    11221 gtggcggcgc atgtaatcgg tgatctcgtg gaccctctgc ctcgtcaggt cgaaggcggg
    11281 gtcccggaag aggtcgacgg ggccgatcag catccctttc gcgatctgga atccggcggc
    11341 tatgacccgg ggggggccgt cgaaccggct gcagatggcg tcctcgaagg cgcagggcat
    11401 caggggaccg ttgtggctcc ccctcatcca gccgctgacg aggtggccga gggagaaggg
    11461 ctccaagacc tccccgaggg cggggagccc cgactgcgct ctgaccaggg cgacggggtc
    11521 gtcctttcct acgtaggtcc cggcggtctg gtagagcttc tcggtgctga cgacggcggc
    11581 gggctcgtcc tcggggagct tccccgcctt ggggtagacc ctcttgatga cgtacctgct
    11641 cttggcgccg atgagggcga ggatgtcgta catctcctcg ggggagtcca tcatcacctt
    11701 cttgtgatcc atgatgtccc agacctcgaa gacgaacccc tggtggatgc tggggtcgat
    11761 gacgaggcct gcggtgttga aggggtcggc gaatatcctg aagatcggga ggttgaaggc
    11821 ccccggctcc gtcttgtcca tcatgaaggc gactaccggc tcggccgccc tctcttcaaa
    11881 ctccatctcg gcgacccccg gccccatccc cttgatgttt ccgcagaagg cgtcgcagag
    11941 gaggtcttgg cccgcgccgt agagcttcag ctccttcgcc ttctccgtcg ccttgcacat
    12001 cacctcccag gcgaggccgt ggatctcggc gctgtcctct ccccgctggt gggtcatcat
    12061 gagctggagg tcgtcgccga cggacatcac cttgaagtcg atgatctcgc ctttcgcctt
    12121 ctcctgggat aggaccgcct cggcggtctc cttcagtgcg gggtggacgg tggcgtggcc
    12181 gggccatcct ccgacgtctg ccttgatcaa gctgatggtg attttagtca tttacagtcc
    12241 cctctctccg gtcgacccct cgaatcgaaa tcggcgttca tcagcggcgt tccgcccctg
    12301 gccgatgacc gacgggggag gtttgattcg gatcttgcca accggccgtg gtgattctgc
    12361 tggatgaggc tgtacctttt gccttttacc tgttacggtt ccgtttttta gaatgggggg
    12421 gcgaagagcc cgctatgtcg gtatctcccc tccccgccgg ggaccattcg ggcataaaga
    12481 gagtttatta ccggcgaggt gcatttatcc tcccctgagg agagttggag gatatggaag
    12541 tcatagcgaa atattatgtc gagaccgacc ttccgatcga gaaggccgcc gaggctatag
    12601 ccgccgagca gtccaccggg acctggaccg aggtggagcg ggagaggctg gcaagagacc
    12661 tcgccgcccg ggtcgtccgg gcggagggga acttcgcgta cgtcgccttc cccgaagagc
    12721 tcttcgaggc cgggaacatc ccccagtacc tctcggtcgt cgccgggaac ctcttcggcc
    12781 tcggcgccct gaagaaggtc aggctcctgg acgtaatctt cccccagagg ctcgtcgaag
    12841 cccaccgggg gccgaggttc gggataaagg aggcgaggaa gatcctgggg gtcttcgacc
    12901 ggcccctggt ggggacgatc ataaagccga aggtcggcct cagcccaaaa gatacggcgg
    12961 aggtggcggg gcaggcggtg aggggcggcc tcgacctgat caaggacgac gagaccctga
    13021 cggaccagtc cttctgcccc atcgacgaga gggtggaggc ggtgatggcg gagctctcca
    13081 aggtggagga cgagacgggg aagaaggcct tctacgccgt caacgtcacc tccggcgccg
    13141 aggagatcct ggagcgggcg gaggaggtcg tcggtcgcgg cgccaacatg gtgatggtcg
    13201 acgtcctcac caccggcttc tcggcggtgg aggtcctggc ccggggggtg aaggtcccga
    13261 tccacgtcca caggacgatg cacggcgcct tcacccggga taggcgtcat gggatatcga
    13321 tgctccccat atcgaggctc gtccggatgg ccggagggac gaacctccat accggaagct
    13381 acctcggcaa gatggccggg gaccgggagg agaatgacag ctgcagagac gccctccggg
    13441 aggagtggta cggcctccgc cccgtcttcc ccgtcgcctc cggcggagtt catcctggga
    13501 tggtccgccc gaacctggat ggatacggca ccgactgcat aatccaggcc ggcggggggg
    13561 ttcacggcca tccctccggg accacggctg gcgcgaaggc tatggtccag gcggtggagg
    13621 cctgggtggc cggggtcacc gcggaggagt acgccaggag tcacaaagag ctggatgcgg
    13681 cgttgaagtt ctggtcccgc tgagatattc cagagcggga gccgatctcg cctaaaaccg
    13741 aagagtttat attgttacat gtaatgaggt cagaacgttt atcacagctt gagtgatggt
    13801 agcgctgtct gaaaacggta aggatgtagt gagggtagag ggatcagatg actagacacg
    13861 agatcttatc gaagatcaag caagcggaag ccgatgctca agccagcatc gagcgggccc
    13921 aacaagagcg agacaagcga atcgccgatg ccactgccga agcaacgaac attgtgagga
    13981 ccgccgaggc tgaagcccag cagtactttg agaagcgtct tgccgatgct gagaaggggg
    14041 ttcagtcgaa gaagcaggcg atcatcgacg acggcatgaa aaaggtcaat gcaatgaaga
    14101 gcggcgccag cggcaaggtg aacgctgcag tcgaacatct actgaaggag tttatggggt
    14161 tgttgcatgc ttagacccgt tgagatgtgt cgtgttgtcg tcgccgggtc gaataaggta
    14221 atagaacctg tcatagagaa gctccacgag ctcaacctgc tgcacctcgt caactattct
    14281 ggcgctgatg aggccttcga gatgggcaag cccctgggaa agtcgtctga atacgccgaa
    14341 cagctgatta agctccggtc catagagagg tacctcggga tcactgacaa gatgcctgac
    14401 aagcagttcc ccgagtctca ggtcctctcc cagatcgacg gaatcctcga taacgtcgga
    14461 gacgacgtca tcgccaccgc cgagcggatc gctgagatcg agaacgaagt caagatcaag
    14521 caggatcagg ccaaggcttt gaagcccctg gcggggctga ccctcgatat gcagcttctc
    14581 cacggctaca gctccctggc ggccttcgtg gggaccgcag catcccccgt cgaggcggac
    14641 gtctccaagg tctcctacgt caatgaggtc ttcacctcca cctccggcgg caccaacgcc
    14701 gtggccgtat tcgtcccggt ggagaaggcc ggggaggtct cgggggttct ctctgagcat
    14761 gggttctctg agatatccgt accccgggga atgactggaa ccatcgccgg tgccataagc
    14821 accctcgagt ccgactccaa gaggctggag ggggagaagg cgccgctcca gaagaagcta
    14881 aacgacatca gggcccagca cgaggacctg atcctggcca tcgacgagca cctctccatc
    14941 aaggcccaga aagcggagtc gcccctgagg ttcgcctcca ccgacaacgc cttcgtcatc
    15001 gacggctggg ttccgacgga ccagttcggg gcgctgaagg gagagatcga gagcgttgca
    15061 ggtggacggg tccaggttga gaggctggac gcctccgagg ccgataagct ggtggaggcg
    15121 ggggaggaca tccccacaaa gctgaacaac cccggcgtag tgaagccctt cgagctgatc
    15181 acccgcctct tcgcgatccc cgagtacaag gagtacgatc cgacgcccct gatattcgtc
    15241 ttcttcccgc tgatgttcgg gctgatcttg ggcgacgtgg cgtacggcct gatagtattc
    15301 atcgtgatga tgatgctccg gcgaaagttc aggactcccg gctggcagtc tctgatcaac
    15361 atcgtcacca tatcgagcat atgggcgata atattcggga tcgtcttcgg cgagatattc
    15421 ggtccgctcg gactgtggga ctacatcttc agtggtgccc actatacggg gctcctgggg
    15481 acggtggctg acccgcatca cgtcggcgtc gacttcggct ctggcttcta cggtcccctc
    15541 gggaggatag gtcccgagat cggcagcttc gctggcgggg tcttccccat gtacaggctg
    15601 gcgacggcct ctgttctggt tttgatcggc gtaagcctct tcataggcat cgtccacaca
    15661 gggattggtt ccatcctggg agtcaagaac gagctcgctt acggcgagaa gagacatgcc
    15721 tactatgaga ggctccccgt gctcctcttc caggtattct tcggcctcgg cctcctggga
    15781 ttgatcgccg ggggcttggg ccacccgttg gtgctcctca gcgccctggt catcgtcata
    15841 tccatcatca tgatggtcag gggacctgaa ggggtcatgg gcgcgactca cataccgttc
    15901 tttgtgagca acctgatatc ctacctgcga cttctggcca ttggcctcgc gtcggtgggc
    15961 gtcgccttcg ctgccaacca gctcggtttc tacgtcttga tgccgatgct cagcggcggg
    16021 gcgcacgatg gattcacgac gatcgcgatc gtcgtcggca tagttgtgtt gattatggtt
    16081 cacttcatca acctgctctt ggggatcctc tccccgttca tgcaccctct caggttgcat
    16141 tacgtcgaga tgttcaccaa gttctacagc gcgcatggtg gtggagtcga gtattcgccc
    16201 ttcggacgtg tccgaaggtt cttgagagac tgagcggtta ggattagtta acggagggaa
    16261 aagaaaatgg cagtagagac tataactgat ggtggagtgc tgtacggact tctggctgtt
    16321 ggcgcaggtc ttgcgactgg acttgccggt attggtgcgg gcgtaggtga gcagggtatc
    16381 ggagctgctg tggtgggcgt cgtggctgag gagcctggct tcctgggcaa gggtctcttc
    16441 c
//
LOCUS       DASM01000066           41885 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_11555, whole genome shotgun sequence.
ACCESSION   DASM01000066 DASM01000000
VERSION     DASM01000066.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 41885)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 41885)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..41885
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_11555"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         7714 a        13257 c        12926 g         7984 t
ORIGIN      
        1 agatggccat ggggctgtct acaaaggtct ttaccccctc gagccttccc ctctcggcgt
       61 agtgattcag ctcgtaaatt atctcctggg tacggccgac ggcaaaggcg gggatgacga
      121 cgtttccgcc gcggtctatc gtctccagga tcacctccat cagcctcttg ccccgctccg
      181 ccatatctcc atgatcccgg ttcccgtagg tcgactcgat caccagccag tcggcctcct
      241 ccaccctatc gggatcccgg aggaagggag cgccgcgccg ccccatatcc ccggtgaaga
      301 gcagcctccg tccgctgacg gagagctcca ccatcgccga gccgatgatg tggccggcgt
      361 ccaggaacct cgccgaggcg cctccgatat cctggacttt ataataatcg acgggcctga
      421 accgcctgag ggcgtcgaag acgtcttggt ccgtatagag gggaggtcgg ggctcgccat
      481 ttcgctggtc aggatggcgg ctttcccggt ctgcctcctc tttcatcagg taagcggagt
      541 ccttgagcat gatctcgcag aggtcttcgg tggcgggggt cgagtagacc tctccggaga
      601 acccctcctt gaccaggagg gggatcctgc cgctgtggtc gacgtgggca tgggtgagga
      661 tcagcgcccc gagagagcgg ggatcgtgcc cgaacccccc ccggttccga tccgaatcgc
      721 gcccctgaaa gaggcccgca tccactccga cccggatatc accggcgtcg aggacggtgt
      781 gggagcccgt aacgccggcg gccgccccgt agaagtgaag cttcataagg aagtcaaaag
      841 cgtcatctcc agattaaact tccggagagt gagattttag cgggtcaatt aaataccacc
      901 agccatcttc gctttgatct ctgccgagtc agcccccagg tcatttcccc ctccttccca
      961 aacccgaggc cctaatcccg ccctcccgag aggagggccg ctcctctatc gggcttaact
     1021 atttttatct ccccttccaa acctcggaga aagaggtcct gaccatgaag tttgcaagcc
     1081 acgtcgagga gatcgacatc tccgggataa gaaagagctt cgagggggcg gggccgggag
     1141 cgatcaacct cggcctcggc cagcccgact tcgacacccc cgaccatata aagcgggcgg
     1201 cgatcgaggc gatcgaggag gggtttacgg gctacaccat gaacctcggc atccccgagc
     1261 tccgggccgc cctctccgag aagctaaggc tggagaacgg cctcgattac gcgccggggg
     1321 agatcatggt gacgagcgga gccagcgagg cgctctttct cgccatcgcc tccgtcgtcg
     1381 aggaggggga ccaggtcctg atgggcgacc ccggcttcgt ctcctacgcc gccctcaccc
     1441 gggtctccca gggaaggccc gtcgccgtcc ccatggacga gcggtttcac ctcaccgccg
     1501 aggcggtcgc cgagagggtg acgccgagga cgagggccct catcgtcaac tcccccaaca
     1561 accccaccgg ctccgtccag acgagggagg agctccgggc cctggcggag ctggccgagg
     1621 accacggctt cgcgctcata tccgacgagg tctacgagca gttcatctac gagggggagc
     1681 acgcaagccc cgccctctac tccgagaacg tcatcaccat caacgccgtc tccaagacct
     1741 acgccatgac cggctggagg ctcggctacc tggcggctcg gggcgaagcc ctggagaaca
     1801 tgctcaaggt ccaccagtac gtccaggcct gcgcctcctc catcagccag agggcggctc
     1861 tagccgccgt cagcggcccc caggactgcg tctggatgat gagggaggag ttccggcgga
     1921 ggagggacgt cctggtggag gggctccggc ggatgggggc agagatggag gcgcccgccg
     1981 gcgccttcta cgccttcccg aaggtgggag acggcgacgc cgtatctgcg aggctgatca
     2041 aggcaggggt cgtcgcggtt ccgggatctg ccttcggatc ggcggcaaga gaccacatcc
     2101 ggatctccta tgccgcctcc atggccgata taaggacggc cctggagagg atgagagaaa
     2161 ttctttaaat aggagctgca gtattcgtcc ggaaggtgac gagatgaaag agcaggtgga
     2221 ggcaagagag ctgaaagagg gaaggtacgt tatcatcgac gacgagccgt gcgtcatcaa
     2281 gagcatagcc aagtccaagc ccggaaagca cggcgccgcc aaggctaggg tcgacgccgt
     2341 cgggatcttc gacggccaga agaggtccac cgtccagccg gtgacggcca aggtctacgt
     2401 ccccatcgtc gagaggaagt cggggcaggt cctgacgatc ggggagagct ctgcccagct
     2461 gatggacctc ggggactaca gcaccatcga cgttcccatc accgaggagg accgggagaa
     2521 gctggaggag gggaaggaag tcccctacct gctcgtcatg ggcaggacga agatggacat
     2581 ccgttaacct cgatgttcaa caggtccaat tttgcggacg ccgacgccga cccggaggtg
     2641 gcggatttca tcatcgtagg ggctcccttc gatgccacca cctgctttag gtccgggacc
     2701 cgcgaaggcc ccgaagccgt ccggcgggca tcttacaact tcgagactta cattcaccgc
     2761 cactccatcg attttaacga cctcctggtc gccgacctcg gggacctcta cctgggggcg
     2821 gaccccgccg acgccatgga gacgatccgg gagtccttcg gcttcatccc cgagggggcg
     2881 acgcctatct tcatcggcgg agagcactcc ataacgcctt cgattgtcgc cgaggtgacc
     2941 cggaggcgcg gcgagatcgg cgtcgtcgtc ctcgacgccc acctcgacct ccgggacgag
     3001 tacggtggga cgaggtatag ccacgcctgc gccagccgtc ggatcctgga ggtggagggg
     3061 gtagcaggat acgcctccat cgggattcgg tccggcgacc tgaaggagta cctctacgcc
     3121 gaggagcggg gcgtcagcta ccattccagc gacgaggtcc gggagcgggg gatcgaggcg
     3181 gtcctggagg aggccttgga ggaggtgggc tgcgacgaga tctacctctc catcgacttc
     3241 gacgccatcg atcccgcctt cgctccgggg gtcgggaccc ccgagccctt cggcctctcc
     3301 tcctgggacg ttcggtacat tgtcgagaag gtggcgccaa gggccgtagg cctggacgca
     3361 aacgagatcg ccccccagta cgacgggggc cagacagcgg cgttgggcgc gaagctgatc
     3421 cgggagttca tcgcctccaa ggcggcgagt aggtagcttt tcgaggctgg ggagatcagt
     3481 aaattaagac cgccagctct gagacctggg agatcttctt gaaatctcga tcccgggata
     3541 tcagcctctc ggcaccgttt gttatagcga tgcccgcaat caaaacgtcc agggcgttca
     3601 cgggggtccc ctggctcagg agctggccca taatagcgcc agccttttcg gcagccctca
     3661 tctcgaattc caatacgtcg atgccggaga aaaatcgcct gaagaactgc tcctccctct
     3721 cagcttttct gtggccgatc ccggcgaaga tctcgtagca gcttatcatg gtggtggccg
     3781 cttcctcgcc gacgaattct ctaatctctt cagatcctct gaagaggtcg atgagggcgc
     3841 tggtgtcgag gacgatcata ccctcgacct cgatagctct ctgatcttcc ccgagtcctc
     3901 ttcaagtccc ctcagcagct cctcgtcctc gatggcgccg aagtaatcct cgaggttgaa
     3961 cttctcccgc ctcatcaacc tatcgatgac gtcgctgaag ctctctccct ctctcttttc
     4021 gcgcttcagc atctcgtagg tctcctccct tatcgaaagg gtcctggtac ccatgaatct
     4081 gtgtatgtat gcacagagat atatcagctt cgccttccag ggctcggagg gtcggatccc
     4141 tctataacct cctctcttgc gaggaggctc gagatatatt cccccggcct tcggcctaga
     4201 ggtccggcga gatggctccc gccttgacgg cggccagatg gcggtcctgg gagcgatagc
     4261 tcttccggga gttcatcggc gcaaaatgcg catcgaatag gcggatcact cctcgccgaa
     4321 gtagtccaga tcgaccctct tgaccctgta ggtggcgacg tcggcgacgc cgatgtcgtg
     4381 gtcgatcatg aactgggcga gctgctctcc atcgatcagg actatcttcg gctgctggat
     4441 ccccttgacg taatcgttcg cctctttcgt gaactgagat gtggtgatga agaccccctt
     4501 ccctgcccgg tttcccatca ggcttccggc gaaggcctgg accgcgggcc tgcccaccgt
     4561 cccctcccac ctcttggcct ggatgtagac ggagtcgagg ccgagctcat cctccttgat
     4621 gatcccgtcg atcccgccgt cgccgctcct gccgacggct tccccggcat ctcttcggga
     4681 gccgccgtag cccatcccca ccagcaggtc caccaccagc ctctcgaaga actcaggaga
     4741 gcatcccatt atccgatcga gaagatcctg ggcgagatcc ctcctcaggt tctggtagcc
     4801 ggcctccagg atctcctcgg gggttctcgt cttctcagtg acatcgtcgt tgagatcgct
     4861 ctggccatct ttggaggatc cacgatgaaa ctcctgaaac tctggaaact gctcaagaaa
     4921 cttgacgttg atctccgccg ggtcggatct caggatcgag agacttcgat cgtttatccg
     4981 gaacttgccc ctccccgtgc tctccaaaag tcccgccttc ttcatgtacg tcctggccca
     5041 acccacccgg ttggctagct tcgcctgcct tccgctgggg aggagctcgc gcctctcttc
     5101 gtcggtcagc tcaaactgat ctgccaggct ctctatcacc tcatgaaggc tgtgctcttc
     5161 gccatctccc aaggccttca agagcgggag catcaagctc tggaaatctg gaaccgccat
     5221 ctctgccgac ctcgccgcct ttcctatctc agttcagata attaagttct gtgctgtagg
     5281 tagtctgttt tagaattctc actcctcccg agaaagcctt atacctatcg aagcagatag
     5341 ttaataactg gcggaaaata aatttccatc tctgccgaaa tccaagttct atgaacctcc
     5401 cgcccaaccc cgcctatcct cgaaaaagcc ccaggttatc ctcatgaacc ctgaaaaaag
     5461 cgcccccttc gaccgtttcg cctccctctg cgagaggctg gagatgacct cctcttccac
     5521 cgagatgagg gacgagctgg cggagttctt cgccgggctc tcgccaaagg acgtgaggat
     5581 ctcctcttac tttctcctgg gcaggatcgg gccgaagtac gccgacgtgg acctatcgat
     5641 cggggagaag accgccatcc cggtgatctc ccgggccttc gacctggacg aggaggaggt
     5701 ggagaggagg cttcacaaga cgggcgacct gggggatgtg gccgagtcgg cttgcactag
     5761 agggcgaagc tccctcgccc tggaggaggt ctattctctc ctccaccggc tgaagcgctt
     5821 ctccgggccg ggaagctcgg agaggagggc ggatcttctg gcagggctgc tcgggagagc
     5881 ctcggcccgg gaggcgaagt acattgtccg gatatccatc ggctccctcc gcctgggggt
     5941 cggcgagatg accatcatcg gcgccttcac cgcggccttt attgaaggga tggaggacga
     6001 gggggtggtg gaggacggct acaacaagtg caccgacatc ggagcggtgg gcgagtcgat
     6061 gtcgcgatac ggcctcaatg gcgtccggcg gttttcgatc tctctgggaa ggccggtcaa
     6121 gatgatgctg gcccagaggg tggggggggt ctccgagatc ctggagaagg tggagggcga
     6181 ggagatcgca gccgaggtga agtacgacgg cgagagggtc caggtccaca agcggggcga
     6241 gagggtccag ctcttctccc gccggctcac ggatatcacc agccagtacc ccgacgtcgc
     6301 cctcgccgcg aggtctgcag ttcgggcgaa ggaggcggtc ctggacgggg agatcctggc
     6361 ctttcgggag ggtaagatcc tccccttcca ggagctgatg cagaggagga ggaagtacga
     6421 cgtggaagag taccgcgaaa aggtgcccgc cgtcctcgtc ctcttcgacc tcatctacct
     6481 cgaagggagg tcccttttga agcgccccta cccggagagg agaaggcttt tggaggaggc
     6541 ggtggaggag tcggatgtcg cccgcctctc ggagaggatc gtctcggagg acttcggaaa
     6601 tataaggggc ttctttgagg aggctctaga gcgggggttt gagggggtca tcgtcaagtc
     6661 cacctcgaag ggctcggtct accagccggg caagaggggc tggcactgga taaagtggaa
     6721 gaaagagtac gaagaggggc tccgggagac cttcgacgtg gtggtggtgg gaagctacag
     6781 cggccgggga aagaggagcg cgcattttgg ggccctccta tgcgccgtct acaacaggag
     6841 agcggacgtc ttcgagacct tcacccgggt gcggacgggc ttctccgacc aggactttga
     6901 ggcgatcgaa ggggtgcttg aggatcaccg agtctctgcg atgccgcctc ggctgaggct
     6961 taagaaggag atggtccccg accagttcta cacgcccgcc gtggtcatcg agatcatcgg
     7021 agccgagatc acgcggagcc ccgcccacac cgcgggagag gaagaagagg gggaggggag
     7081 agggttcgcc ctgcggtttc cccgctttct gaagaccagg gaggacaagg ggccggaaga
     7141 ggccaccacc gttgaggaga tcagggcttt gagatgacca caaacggaga gctggccggg
     7201 atcttcaggg agatggcccg gatgctggag ctggaggagg tggactggaa gcctcgggca
     7261 taccggaagg ccgcctacgg gatccaggat ctggccaggg atgtgggtga ggtctatcag
     7321 gaggggggaa gggccgccct caagaagatc cccggcgtcg gcgagagcat ggcagaccac
     7381 atcgtcgagt acctcgaaga ggggaggatc gagaagttcg acgacctgag ggagaagtac
     7441 gcggagggga taactgagat catcagcctg gagggggtgg ggccgaagac cgccaagagg
     7501 ttcgccctgg agctgggcgt ctcaaacctg gaggagttga ggaaggcggg agaagatcac
     7561 cggatcagaa agcttcctgg ctttgggaag aagagcgagg agaacgtcct cgctgcgatc
     7621 gagacttatc gggagggacg ggagaggatg ctgatcagcg tggccctgcc catagccgaa
     7681 tcgatcctaa accatctcca ggagatctct tcggtggaga ggataaacta cgttgggtcc
     7741 ctgaggaggt ggaaggagac gatcggcgac gtggacatcc tcgtcgcctc cggggaggcc
     7801 gtggaggtga tggaaggctt cgtggggatg gagggggtgg ccgaggtcct ctcccggggg
     7861 tcgactcgtt gttcgataat cctggaggag ggggtccacg tcgacctccg ggtgatccca
     7921 aaggagagct acgctgcggc gatgcagtac ttcactggaa gccgggagca caacatagcc
     7981 ctccgaaaag aggctctggg catgaaccgc aagctctccg agtacggcct cttcaaagag
     8041 tcgggagatt cgataccttg cgaaaatgag gaggatatct acggagagct ggggatggat
     8101 tacatccctc cggagctgag ggaggaccgg ggcgagatcg aggcggccaa ggccggaaac
     8161 ctccccgacc tcatctcggc cgaggacatg ttgggcgacc tccaccttca caccaggtac
     8221 agcgacggca ccgccaccat cgaggagatg gcaaaagctg cccaggagaa ggggtacgac
     8281 tacatcgcca tcaccgacca ttcaaagtcc gctcggatcg ccggggggat ggacgagaag
     8341 acgatcagaa agcagtgggc agagatagac gatatctccg aaaagtgcga gatcagaatc
     8401 ctgaagggcg ccgaggtgga gatcctcaag gacggaagcc tcgactactc cgacgagatc
     8461 ctggacgagc tggagatcgt gatcggatcg gtccactcca gcttcaagct ctccaaaagg
     8521 aagatgaccg accggatcga gagggccctg gaggatgagc atctggacat cctgggccac
     8581 ccttcgggaa ggctgatcgg aaagaggcct ggatttgagg ccgattttcc agagatcttc
     8641 gaggctgcag ccgagaggaa gaaggttttg gagatcaacg gccagcccga gaggatggat
     8701 ctgaacgacg ccgcgatcat cgccgccagg gagaagggcg caaagttctg catagatacc
     8761 gacagcatct ctcctgcaaa tttggattgt atgaagtacg gcctggggct ggcgagacgg
     8821 gggtggctga cgagggagga ggtggtcaac acctacgatc tcaaagcgct gaagaaggtc
     8881 ttcaggagga tccgatgaaa gttcaacccc tggaagtcca ccctattttt tcagcctctc
     8941 ctctctccca tcagagcctc aaaccccacc agcacgtcct gcatctccga ggagatcgcc
     9001 tcctgacgcc tttttcgata ctggctggac agctcattga ggtgctcctc gatgttcctc
     9061 tccgctgcct gcatcgacga gagcctgctg gcgttctcgc ttgccaggga ctccacgaag
     9121 gcccggtaga ggctggagaa gatctgctcc cggaccaggg aggagaagag ccggtctggg
     9181 tccatggaga aggtgggcag agaccgggag agccacctcc tctcggcgag ttcgttcagc
     9241 caaccggcat cgagggggag gaggcgggcc atggatgggc ggaaggcggc gagaggtccc
     9301 gacgccggcc ggttgtagaa gaggacgatc cgatcgaggc cgccctcgat ccgccactcc
     9361 tcgatcttta ccagaacctc cgccatcacc tcggttatgc cgataaaatc tcctgagaat
     9421 gagaattgag cctcgatcga gaggccccca tcttctaggt ggccgatgac ccgctcgccg
     9481 accgccacgg ccctcgtctc ctggtggtcc atctcggcca gttcggcgag ggcaaagctg
     9541 gcgatctgct cgttgaactt gccgcagaga ccctgctcgg agccgaagat caccgtcccc
     9601 agaagccctc cttcccttgc aggggagaag gctggaacga tcttcccgct cttcatcatc
     9661 accgcaagtc ccatctcgat ggacctggag tactcggcga gagattcgac tgcccgctca
     9721 tattcgcgga tgttcgtcga gctgatggcc ttcatgatct tgaccaccga ctgcagctcc
     9781 cccgcatttt tgatctttct cttgacccgt tcgagctctt ccatgatccg cctcccaaat
     9841 ccagctctca cgcaccgagg gcatctttga aggtcctgag gagcatctct cgatcctcgg
     9901 ggctgagctt ctcccccgat ccgatcctct gaatgacctc cggatgcttt tctcgaaggg
     9961 cctttcttat cgtctccttc gccgaggcga cgtccccgag ggggactttg tccagaagcc
    10021 ccgccgtcac cgccaaaagg accgcgatct gctcgggggc ggggatcggc tcgaactggg
    10081 gctgcttcag cacctcccgg atcctctgtc cccgttcaag ggtctttcgg gtatcctcgt
    10141 cgagcctggt cccaaaccgg gtgtaggcct ccaggtcctc gaactgagag taggtgagcc
    10201 gcaggtcgcc cgcaaccctg gagtaggcgg gaagctggct ttttcctccc acccgggaga
    10261 cggacatgcc cacgtcaact gccggaagga tcccctttcc gtaaagctgg ggggagaggt
    10321 agatctggcc gtcggtgatg gagatgaggt tggtggggat gtaggcggag atgtcctggg
    10381 cctgggtctc gatgatggga agggcggtga gagagccgcc gtctttgagg ttcgttgccc
    10441 tctccagaag cctggaatgg atgtagaaga tgtctcctgg gaaggcctcc cttccgggcg
    10501 ggcggcggag gagcagagac agctcccggt aagatcgggc gtgccgggtc agatcgtcgt
    10561 acacgatcag aacgtcccgc cccctatcca cgaaatattc ggccatggtg gtggcggagt
    10621 agggagctat gtagtttaag ccgggcggat cgtccccgct ggcgacgacg gccacgcagt
    10681 tatccatggc gtcgttcttt cggagatctg cgatcgtctt tgccacctcc gacttctcct
    10741 gacctatgga tgagtagacg caaacgacgt ctttatccct ctggttgatg atggcatcga
    10801 tggcgacggc ggtcttgccg gtctgccggt ccccgaggat cagctctctt tggccgcggc
    10861 cgatgggaac catggcgtcc accgccatca cccccgtctc cagggggacg ttgacgggag
    10921 atcgatccat aatcgccgga gctaccctct cgatggatct tctctccgag gtcgcgatcg
    10981 gctcgccctc gtccaggggc ctacccacga cgtccacgac ccgccccaga agctcctcgc
    11041 ccaccggcac ctccatgacc tttccggtcc ggtggaccac ctgcccggcc ctgaccttct
    11101 cgctcttgtc gaggaggagg accgccacct cttcagggtc gaggttgaag gcgatcccga
    11161 gccggtcgcc gggaaataag acgacctctt cagacctgac gtggggcagt cctctgatgc
    11221 gagcgatgcc tttgcccacg aaggatacta ccccgatctc ctcgaacttc agctcgagct
    11281 ttcggctttt cagggtgtgg ccgacgacgt tgaaggcgta gtccagggcc tccctgactg
    11341 cctcgtcccc cttcttcgtc tccatcatct gacccgcttc tctttaggag ctctctggtc
    11401 gaggacgcgg gagaagtcgg cctccaggga gtcgagatat ccggcgatgc tccaggagat
    11461 ctccgcgttt cttgcggaga tctccatccc ggagatcaga tcctcgtcga tctcgaaatg
    11521 gaccctcacg tcggcctcgg tccgatcccg caagacctcc ttgatcttct cctggacgcc
    11581 ctctgggatc tcgaaggcgc ttctcaagac gatcagctgc tcaggatact tataaaactc
    11641 cttgaaagcc tccttctccg aatcttccat cttctccagc cgttcgacga aggtccggat
    11701 catctgctcc tccagctcct cgtcggctag gtctcggagg acgcggcgag atatggcgta
    11761 gacctgcctt ccggtcctct ctctcagatc gtcgaggaag gccttcttag agctctccac
    11821 ggccccatac cacccggcct tgctcctctc gacctcggcc tccgccttct tctccaggtc
    11881 cgccttgaag ttcttcgcat catccttcgc ctttatcagc ctctcctcgt gctctgccga
    11941 gatctccagt ctctttttcc tataggtctc cgcctcctcc tcggccgccc tcttcttctc
    12001 ttcgacctcc tccagccggg aggcgatcaa ccgctctcgc tcgtccatca gcttcaggat
    12061 aggggcgtag aggaagtgct tcaagaggag caccaggatc agaaagttca cgatctgggc
    12121 cagagtggtg aaatagtcta tctgcaacgc tatccaccca tgctgatcac gtagtcccag
    12181 aacggattgg agaagatcag gattatcgat ataacgaagc agtagatggc ggtggactcg
    12241 atcatcgcaa ggcctacgaa gagggtccgg gttatggtgt tcgcctcgtc gggctgttgg
    12301 gcgatggcgc tgagggcgcg ggagagggcc cagctctctc ctatcgcggg tccgatcgag
    12361 cctatggcca tcgtcaggcc ggatataatt atggaagctg tgcctatcgt atctatcgca
    12421 gccatgtcaa gttcctcctt caggttctcg cttcgggttc tcttcagatt cctcttctat
    12481 ttcgggtttc tcttctattt cgggttcctc ttcgaccgcc ttctcgtccg agcttgccga
    12541 tattatgtat accgtggcca gaaccgcgaa gatataggcc tggacctggc cgatcaatag
    12601 cccgaagagg tccattatta cgggcaaaaa taggggtgca agggagatca agacggcgac
    12661 gaggagggtc tggctcatca tgttcccaaa gagcctgagg gcaagggcca ccgttcggga
    12721 gatctctcct atgacgttga aggggagcat cagcggcgtc ggctggacgt accgcctgaa
    12781 gtaatcagag gcgccctgct gcaagatccc aaagaccggg accgcaaaga agacgcaggc
    12841 ggccaaagcc gtcgtggtgg agagagagct ggtcggcgac cggtagcccg gcacgatccc
    12901 gaggatgctt gagagggaga gaaacaggaa gagggtgccg gagaagggga gataggggtc
    12961 agaatccctg cgggctatct cccggatctc gcctctcatg tagctgatga tagtctccag
    13021 ggcgacctgc cttcgggaga aggggggcgt cggtgaaagg tggcgggtcg atgcccaggc
    13081 aagaatggcc aagattccga tcacgatcca tgtgaagacg atggtctcat tgatggtgac
    13141 aaactcccac ctccagagga ccgcctcgtc ggggctcagc tcgaccattt ctagatcccc
    13201 ctcctttcgg ggtgccgacc aagacggctg acgatgacga tcttcgtcag gatgaagccg
    13261 gcaagggaga aggccagacc caataggccg gtttcgacga cgagataaaa gccgaagata
    13321 gagactgctg accgaaagag gaagcttccc agggtcagga gctctggact cttagattgg
    13381 gggagccgat ttatcgtcag ccagaggcct ccgaagaaga agatcccaag ccctatccca
    13441 aaggtgaagc tgaacatggg cagaagatgc tcattcatct gagtttctcc cctcgatctc
    13501 cgtctgctcc tttctgaccc agtaccaggc gttggcgcat ccgacgatca ggccggcgga
    13561 gaggaagacg agggtccagg agatgggacc agggtatcgc gtgtcaatcc agactccgat
    13621 ggccacacct acgagcgtgg gaaccgccac cgaccagcca acgacgccca tcatcccaag
    13681 gccgaaccag acccctcgcg ctcgctcctt ccgagcctta atcttgcgct cttcttttgc
    13741 gcctatctct tcggcgaaat cttcccggtc tttttccatc gtcccttccc gatctacatc
    13801 ttctcaaaga cgctccttgc gaagtccgcc tcgagcttag ccaggatggt gcgggtcttc
    13861 ttctccctct cgtccagggc ccgaaactcc tctcggacgg ttctcttcag catacccagg
    13921 tccctgctac gtatcgcctt tctagtcgag accgtgaccg ttgaggcgca ttttaccaga
    13981 atcccctcgt cgatggcgag aaactcctcc ccctcgccgg aggtgaagga gaagattccc
    14041 ggcaccatgg cggctacaaa gtcgatatgc ctcggaagga tgcagaagga gccgtcctcg
    14101 gcctcggcca ccaccttcgt cacctcttcg tcgatgagga ccttcgttgg gagaagaacc
    14161 ttcagcctca tttttccgcc tcttcgatat ttccgatcat gtatagcgac ttctcagggt
    14221 agtctctgaa ctcgtcgttc aatatccgct cgcagccgtc gagggcgtcc tccaggctga
    14281 cacgccggcc ctcatgcccg gtgaactgct cggtcgtgaa gaacggctgc gtcaggaagc
    14341 gttcnntccg ctcgcagccg tcgagggcgt cttcaagccc caccatcttc ccttcctggc
    14401 cggtgaagtg ctccgttgtt acgaagggct gggtcaaaaa ccgctccagc cgacgggccc
    14461 ggttgacggt cttctggtca tcctctgaga gctcctcaag ccccagcatg gcgatgacgt
    14521 ctttcagctc ctcgtactcg gcgagggttt ttcggatctt ttgggctata ttgtagtgct
    14581 ttgatcctac gatggtgggg ctcagcatcc tcgacctcga ttggagggag tctatcgccg
    14641 gatagaggcc ctggctcgcc atcctcctcg ataagacgat ggaggnnctc gaggcccagc
    14701 atggcgatga tgtccttgag gtcctcgtag tgcgcgagcg tcgcgcgcac ctgctgggcg
    14761 agttcaaagt gccggcgacc cgcggtgtgc ggcgtgagta tcgaggaggt ggactgcagc
    14821 gggtccatcg ccgggtagag cgcctcggcg gcgcgccggc gcgagagcac gatggtggcg
    14881 gagaggtggg cgaaggtatg caccgccgcc gggtcggtga agtcgtcggc ggggacgtaa
    14941 actgcctgga ccgaggtgat ggcccccttc ctggtgctgc agatcctctc ttcaaactcc
    15001 gccagctccg tcgccagagt cggctgataa ccgaccctcg aaggcaattg gcccatcagc
    15061 cccgatacct cagagcctgc ctggacgaaa cggaatatgt tatcgatcag gaggaggaca
    15121 tcctgtcttt tgtcgtccct gaagtactcg gccatggtga gggcggcgtg gccgatccga
    15181 aatctcgccc ccggcggctc gttcatctgg ccgaagacga ggatggtctt gtccaggacg
    15241 ttcacgtcct ggatctcccg gtagagctcc tctccctccc gagatcgttc tccgatcccg
    15301 cagaagatgc tgatcccctc gtatcttccg gcgatgttat tgatcatctc catgatcagg
    15361 acggtcttgc cgacgccggc tccgccgaat aggccggcct tgccgcccct ctccaggggg
    15421 gagaggacgt ctatcgcctt gatcccggtc tcgaagatct ccgaggtggt ggtccgctga
    15481 gatatcggtg gaggcctctg gtggatggag cgaagctcct tcgcctcggg actttccttc
    15541 cggtctatag gcgagccaaa gacgttgaag accctgccca ggaggttatc ccccacagga
    15601 actgtcagcg gctggccggt gtcctccacc gtagagcccc gagccagccc cgaggtgggg
    15661 gtgagagcga tccccctcac cgtctcggag tcgagatgga tcagcacctc tatcaccacc
    15721 tcgccgcctt cgccggccaa gaggacgctg ttgatctctg gaagcgactc cgtgaacctc
    15781 gcgtctacaa cgctcccccg gaccgagacc acctctcccc ggttggatga ggtcaaattt
    15841 tatctcaccg tcggccttcc gccctctttg ccggaggcgc cgaagtgctc ttttatctcc
    15901 agctccacct caagcttctt ctttttggca tcttccttgt aggagatctc cagggtgacc
    15961 ggctcgtcgg ggttgagggc cactttctgg ctggcgaggt ccatctccac cgcccctctc
    16021 ctatccaggg tgtcggcgag ttcccggagg aaggccgccg cctcagtgaa gctgacgggg
    16081 tattcctctt caatgtcgat tacatccgat ccctttcccg ccttcgcctt ctcttcctca
    16141 ggcctcttct cggcctctgg gccggttccc ttaggcacta ttatcgccat aatacaccaa
    16201 ctcctcggat caaacggtct acaataaagg accgtatgat tgatatatct aacccttccg
    16261 atagcggcgg atcgttcgga tcctccgatg ggctgaagat gatggtccgc ttagccctcc
    16321 gcctcctccg ttgcggatcc gggggctccg cctttgatag ccgtctgcag ctttcgccgt
    16381 cgacgccggt tcaggatgtc cttcattacg tactctcgcc aggtcctctc gcccttcgcc
    16441 atcgagagat gctcgaagtc gtcatcatcg atggcgatgg taatatctca ttattacctc
    16501 aacgctccaa cgcttctttc gtctcgaaga gctcacctgc tggtgtaccc cccgtcaatg
    16561 atcagctccg agccggtgac gaacttcgcc tcgtcggaga cgaggtataa gacgccgtag
    16621 gcgatgtcct caggctcgcc gatgtggccg atgggatgct ttgcgtccag ctctcccttc
    16681 agatcctcga gcgccactcc cagctctagg gactgggcct tgaggaaatc ctccaccatc
    16741 ggcgtccaga tgaagccggg gtggacagag ttgaccctga tgttctcctt cgcgtagatg
    16801 agggcgccgg ttttggccat gatcctgttg gcggccttag tggcgtggta ggcggggttg
    16861 tcctgggagc cgacgatccc gtagatcgag gatatgtaga cgatatttcc gccgccgccc
    16921 tccttcatat aggggatgac gttcttgctg cagaggaagg ctccggtgac gttgacatcg
    16981 aagaccttct cccactcttc cttggtgtac tcgtgggtcg gcttgctggg gccacttatc
    17041 cctgcgttgt tcaccaggat gtctatcttc ccatacctct gatagacggc cttcgccacc
    17101 tttgccacct cctcctcgtc ggttacgtcc aggtgatggt actctgcggc tccgcctgca
    17161 gatctgatct ccccggcgac cctctctccc tcatcttccg tccttccggt gatgatggag
    17221 atggcgcctt cggctgccag gaccttagct atagcctcgc ctatcccgta agtcgagccg
    17281 gtgactatcg cagtcctgcc ttccagccga ccgttcactt tcgctttcat atcatccacc
    17341 atatacgatc tcttcatcca gtttcatccg tgaagcctcc atcgctaccg gcatctccgc
    17401 cagcttccgc ctggaggcct tctccccttc cgtctgagaa cctcgaccta taccgcacgt
    17461 ttttggtctc ctttatatga atagttatcg gagtatcaga gatcacttct gtatttatcc
    17521 agcgacttga gtgctcccat atatcaacga aaaagggttt ttttccgttc taaaccaata
    17581 aagaccgatt ttaagctcgg aaaacttaaa ttattttatg aaagatttag atcccacagg
    17641 atcgaaaaaa gtctctggac tgtaaaagct gtcagcctat tccatcgatc tctccgtctc
    17701 agtcaataga tcccctcttt ttcagatcgc agggctcagg aaagcttcaa aaaaattgtg
    17761 tccgggatct cggccctcac ttttccttcc atcttccgct ctcttcatcc ttctcgtaaa
    17821 ccttcttcac cgcggcccag gcgaccttgt gggcggtctc ttcgcgggat gcatcacccc
    17881 ggcgttcctc ggggtccgcg tactcatccc aggcgttgtt gaaggccttc atgtagatct
    17941 cctgggcgtg ttggggcagg ttatctctga cgcttttggg aagttcgtcc aactcttcgt
    18001 agggcatggc accatcacca gagttatacc atcatcgcat acgtattggt ctttttccct
    18061 ctctacagac gaggtatttc gagaatctga ccagatccgt catggcctgt tattaaatac
    18121 cttcaagtcc tataatatct tcgagaaggc aaggatggtg tacgatgatg gttttagggc
    18181 tggacctggg caggctcaaa gagagtttgg aggctctgga gatcgccgac ctcttggaga
    18241 tggcggagga gatggactgg actgctctct cgtcctcttc ggggggatcg gacctgaagg
    18301 agctgaagag gagtctcaac gaggacgacc ttgtggagac gctcctcctc ttgaaggaga
    18361 tggacctcga cgccctcatc gagagctcca ggagctccag catcgacgag ctgaagaggg
    18421 cgctgaaaag ggtcgacctg aagaaggggg tggctcttct ggggttgata ttgatggcaa
    18481 agagggggct tgaggagctc ggccggcggg ccgaagaggg cgcgagcgaa gtcgagggcg
    18541 aggacgtggc cggtcacccc ccctccgcca agacagagat cgaggtcgag atcgaaggag
    18601 aggccggcgg cgagggagat gacgagctcg tcgcatccgc ggggtctgat aaatatcacc
    18661 gtttggactg ccgactggcg gcgaggatct cgccgaagaa taaggtggtc ttctccgggg
    18721 tcgccgaggc aaaatcccgg gggtacaagc cctgcgccct ctgcaacccc tgactgtcat
    18781 tttcgcagag ttcagccctc ttcgccctcg gtgcccggcg tctaccgacc cttcgccacc
    18841 tctatcctcc gccggagctc ctcctcaaac ccctccacct cctcatcttc gagccggaca
    18901 tcccggaccc cgccgtcctt gaggtagaag agccgcgccc ccagcatcgg ccggaatatc
    18961 tcccgggcga ggtagaggta gaacttcatc tgaaactcgt aggcgggagc gtgggcggag
    19021 gagtcggcga acttgaagtc gacgatatgc cagccatctc tcgtcttcac cagcttatcg
    19081 accctcccga cgagggtgtg ccccccgaag gggcggagga tctggtactc ggtcctcgtc
    19141 ttttcggcgg cctcgatctc cctcaccagg cggtggccct tcagcccctc gaggatggtc
    19201 cagacctccc ggagctcttc ctcggtgaac cttccggcca ggaggtgggg ggggcggcgg
    19261 ggccgctccg ccgatccgag ggcttcgagg gtccggccgg cgacgccgag gctctccaga
    19321 atctggtggg cgagcctccc caggtccttc ggctccaggg gctcctcctc atcggcgagg
    19381 tccgcctccc cggtctccac ctccacctcc acctccagct ctgccgcctc aggcctggga
    19441 ggagaggccg gccccggccg gatgggccgg tgctcctccg ggtctacgag gcgggtgggg
    19501 gagattcgga ggctcccgtc ctcgacctcc atcggatcga tgagggtgag gtccgggagg
    19561 atggcagacc cctcgccccc gggggcgggg ccttcctccc ccgcctccag atccgccgcc
    19621 ctgaccacct cgaggacccc caagaagtcg ggggcaacct ccggggtttc gtcctcgcct
    19681 gggatgaaga cgccggcgag gaggtcccgc cagggcctcg ccccccgcct cttgggggcg
    19741 gatagggtga gggccaagag gtccctcgcc cgggtggcgg cgacgtagag ggtcctctcc
    19801 tcctcttcgg cctcccgcct cctctcctcc tccgccatct cggagaggaa cgtcccctcc
    19861 ccggccttct cttccgggag gagggagaag atgggccctc ccccgccgtc gcctcccctc
    19921 ctcaggagga agggaggctg ggtcgcccga tcgctccagg aggtctcggc gacgatgacg
    19981 atcggaaact cgagcccctt cgccttgtgg acggtcatca ccttcaccgc gtccgtctcc
    20041 tcgccctcga cggaggcggg ctccacgtcc tcgaaaccgg agccttcgag ccttccgatc
    20101 acctcccgaa gggagagccc cgccccgtcc agggccttcg aggagtccat cagcttccgg
    20161 aggtttgcgg tcctcctcct cagggggtca gcgagggcga ggtgggcctc gtaatcgagg
    20221 agctctgccg cctcctccag gatggcggag ggggtcagga ggtctttacg atctgcgaat
    20281 ttttccagaa cctccctcag ccgcccgacc cgcctcgccc tctcggggga gaggtccccc
    20341 tcctcgatcc cgccgagggc gtcgaagagg ggggccccgg ggccgccggt gggtggggag
    20401 cgggggcggc cttctggatc cgtctccccc tccccggcga tggccttcgc cttagccctc
    20461 gccttcagcc gggccaggtc ctggaggtcg aggccgacgg ccggggagag gagggcggcc
    20521 gtcagggcga ggtcgtcggc cgggtctgcc aggactttga ggatcgagac gagcccccgg
    20581 acctcgggga ggtcgaagaa accagcctcc ccgacgacgt agcaggggat ccccatctcc
    20641 ctcagggcct tggcgtaccg gtcccccgtc ccgccccgaa gcttcctcag gaggacggcg
    20701 aagtctctgt atctgaccat ccgcctctcc ggaacgaggt tcacgccgcc ggatccgtcc
    20761 tccttcctca caagaaagtc aaactccctg cccaccatcc tccttatgaa gagggcggcg
    20821 gccttcccct cgtcctcggg gtggaagagg atcttcgccc ggggtaggtc gccggtcttt
    20881 cccgccaccg gcaggggaat aacagggctg tactccggcc cgccggagat gccggctcca
    20941 tcgaagatgc cggcgaagag gcggttggcg aagtcgagga cccccgggtg ggaccggtag
    21001 ttcgtcgaga gctccaggac ggtcccggac cccgcctcca cctcccgcct cgcctcggcg
    21061 aagacctcca cctccgcccc ccggaaccgg tatatcgact gcttagcgtc gccgacgtag
    21121 acgacgcgcg tctttctccc tcggaggagg tcgacgatct ccttctggag gtggtcggtg
    21181 tcctggaact cgtcgacgat gatgtaccgg aactggctgg agagtcgcgc cctcagggcg
    21241 gggctatcca ggaggagatc ccggagggcg aggaggaccc cgagaaagtc cgtccggccg
    21301 agccgatcca tctcccggcg gcgggctccg tcgaagcggc ggaagagctc caggaaggcc
    21361 cggttcgcct cagcgaggga ggcggcgctg gccgggtctt cgaccagggg gaggccccct
    21421 tcgacggcct ccgccggcct tgcggcgatg accttccccc tcccatcgac gccgagggcg
    21481 agggctccga ggaggtcgtc gaggcccgcg accctcagga tcggctccgt cagggcctcg
    21541 ttctcccaga ccgtccgcag ggccaccctc cggacgaggg ccgaagagct ggcggcggcc
    21601 tctacccgga ggccgggatc gatcccggcg agggcgccgt tctccgccag gatccggccg
    21661 gcgaaggagt cgatcgtccc gatccaggcg aaggggatgg aatagaggag gcccgtccac
    21721 cggggatctg tcttctcccg gatcccctcg acgatcctcc tcttcatctc ccccgccgcc
    21781 ttcctggtga aggttatcgc cgccacctcc ccgacggaga tctcctcccc cgcctggaag
    21841 gcctcctcga agatctcgag gtacctcttg gagatggtgt aggtcttccc cgtcccggcg
    21901 ctcgccgata tgaacaggtc gcccttcatc gggacccctc ccccgccatc caggcggcga
    21961 tgacgcaggg ctccacctgg aaggggcacc cccagcaccc tcccgtcgac ccctccacct
    22021 cggcagcccg ccgaaactcc ctcccctcga agatcccgtc cagggcctct cggagctccc
    22081 cgtccacctc ggcgaaggtg gggtctggct tcgacctctg ccggaccatc ctcccctccc
    22141 cggcatggac ctccaggacc cgggagatcc ccggcttccg gacgatccgg aatagagccc
    22201 tcatcgatcc gggccgggct gggacctcgg ggaggccgag ggcgaggagg gcgagggagt
    22261 agagctggag ctgctcccac tcggcgccgg aggcggagga cttgtagtcg gagaggacga
    22321 cctcgatctc gcccttcttc ccgggggcgt agaggtcgat ccggtcgatc ctcccccgga
    22381 aggtgaggtc cccgtaactc tcggcggagc gggggaggag gcctcccatc ctcgatagct
    22441 ccaggctgaa ggggacctcc gccatccggc ggcggccgac ggcggccctg accggatcct
    22501 ccgcctcgaa tctgagatag ccgaggaggt cggaggcgac ggcgccgacg atcctccggc
    22561 ggaccgcctc catcctcctg accttcccgc cggcgaggaa cctcgctgcc gtcctctcca
    22621 cctccccgcg gaccaggtcc tctcctcccc catcccaaag gtcggcgccg ggtggggccc
    22681 cctccccgac ggccctgagg acggcgtggt aggcggtccc cctctccagg ggggtgagct
    22741 cgaggcttcc gggggccggc tcctcgatcc ccagggcgcg ggtgaggaag aaccggaagg
    22801 gacagctccg atagtcccgg agcttcgagt agctgaagct cctccccacc agggtcgagg
    22861 agaccccggg gtcctcgatc ctccaccgga aggggggctc cccggcccgg aggcggaggg
    22921 gatcgacctc tccaaggaga tccgccggga cctccagccg gccgcccccc gccagggctc
    22981 gggcgtactc caccctcagg tggcgccggc tcaggatcgt cggcgatttc gatggctcct
    23041 cctccgcctc ggcggcggga gaaacccctg ccatcctctc cagccagagg gaggggacga
    23101 ggggtgcccc ctcccgggag gccctcggat aggtgacgac ggtccgggag gaggaggcga
    23161 tcgccttccg gagccgggac cgctcgatcc ggtcgtttct caggtggagg tttctctctc
    23221 ccctcccgga ggagaggtcg ttcagctggt agagggggtt tgctaagcgg ctggggaaat
    23281 ggccgtcgtt gaaccccgcc acgaacttca cccgtcggta cctcctcgag gagagggcgg
    23341 gggagaggat ctcgacggcc ccggatcggg cggatccggc ccaggccggg gccccgaggg
    23401 ctgccaccag gatggagagg agccggtcga ggggaaggtc ggcccggccg agggcctcca
    23461 gggtcagggc ggtcctcgtc accacctcct caaacccatc cagggcaggc ccctctacct
    23521 cggagtcggc gagcctcggc ctttgaagct cgatcagccg ccccagctcg gaggcgaccg
    23581 cagcgaggca ggagtcgtcg atcgccgcct ccatcgacgc taggcggtcg aagagctcca
    23641 cggatcggct catgacgagg gcgcaagact cggcccgccg ggccgccgcc gccacctcgc
    23701 cttccagaac cgtctcgtcg gcggcgagga gccgctccat ctcccggagg gcggactggt
    23761 gctctgccag ggcggcgatc cagctccggc gggccgaggc gaggttgccc gccggcagga
    23821 ggaggaggcc ggcggcggag gcgagggcct ccagctcccg gggtgaggcg gggaggcagc
    23881 cgtcgaggag ggcgaggatc agctcggggg ggtatcccga ggccgccgcc ctgaagggga
    23941 gaaggaggag ctgtaccgtc ttcttctcca ggaggctctc cccggcgagg agcctcgccg
    24001 gaactccggc gtcctgaagg gcccggagga tctcgcgccc ccgggtggcg gggtcggcga
    24061 cgacgatcga tatatcttcg gggcggagcc cctcggagat cagcctcttc gcctcccggc
    24121 atagggcgaa ggtctccgcc tccgggtcgg ggcggctttc tacggagacg aggccccgga
    24181 ggtcggaggc cggggggggc ggtccatcca ggaggaagga gtcgaggccc ttcgataggg
    24241 gctcgtcccg ggggcggcat tcgatctcct cgaacccccc ttcggccaga agggcgagga
    24301 actcctccag ccctccccgg ctccagaggg cggggtcgag ggtgatcgtc gcctcctcga
    24361 agaggggaag aaggcgaagg agaaacccca tctccctccc cgggaggtcg gcgaggccgt
    24421 cggcgaagag gcgggtcccg tacctattct ggagttcctc cctcatgcca tccggatcga
    24481 atccgcggta ggcgtcggcc aggtcgaaga ccccccgccc ccggacccgt tcgacccccc
    24541 tccggaccgc cctcgcgaga ttgagcgcct cctcggaggc gaggaggtcg gcgatctcgg
    24601 cctcgcccct gatggcgagg tcccaggccg cctcggcggc ggagcgggcg aaggcggccg
    24661 aagatcggag cggggcgaag gcacccagct catcggcggg gaggcaggag aaggcgtcgg
    24721 cggcgaggag gttcaccacg cccctgctga ggagcacctc gccggggtgg gcgagccggt
    24781 ggaggtcttc ggcgaccttc gagagggtga cgaagtttcc ggcggggacg ggccccttca
    24841 gcgcccgagc gaacctctcg gcgaagctct ccgcctcctc cggggttggg gcgatgaagg
    24901 tgtaggaggt ggggtcctcc cggtgggcct ccgccatcgc cgccatcagc ttctccgtct
    24961 tccccgagag ggcggggccc cgcaggatct gcctcctcaa ctctctcctt ctcctcttcc
    25021 tctgggagat ctgccaccgt cgtatatccc gtttgctgaa tctcgcccga ggggcggaga
    25081 tgaccagacc ggtggaggaa gggcttatga ccccggggct cgatggggtc ggctatggcg
    25141 agccctgagg ataaggcgag ggcggcggcg aagatcctcc cccggtccct ggaggaggcg
    25201 aggggagcgg tggaggcgcg aggcctcctc ttcttgaggc ggctcgaccg gctcgaggcg
    25261 ggcctcgaga gggtagggac tccccgggag gccgagaggt tcgccagggc gacggcgatg
    25321 ttcctccttg actcgctgcc cctgaggccg gaggcctgcc ctttctgcgt ccagaacgcg
    25381 ggcgggaacc gctgcgaagc ttgcggctac gccaagaccc acggcgggga gtgcgacatt
    25441 gagaccagcg ccttcggcca gctcatcgag gcggtcatcg acctcgcggg agagatccac
    25501 gagatccgtg atgatccctc caccggagcc gtcgatcccg aggaagcgaa aaaggagctc
    25561 ggggcctcca tcgcccgatc aagggaggcg gcgaaggccc tttttgccga tatagccgaa
    25621 gccgacgtct ccgggctgat ggaggcgaag agggcctaca tcggggctat cctggaggcg
    25681 ctccccgccg ggatcatcgg atctcgaaag gtcgaccggc ggatcgaaat ggtcgcattt
    25741 aggctggagg gttactggtg aaggccctcg aaaagctata aatcgggaaa aggcggaata
    25801 tatagaaggt gttttcatgg acataagaga gaccttgcgc cggtatccct gcatcgccat
    25861 cttcagcctc tttctgctga tcatgctgat cgtggtcttc tacaccaggt gaggggaggc
    25921 gcgatgcctc ccgccgcccc tctgatcctc cttattagcc ccgccgtaag ccgtcctttg
    25981 ataccccgaa gggacatgcc ttagatcgaa ttccagcatc tctccggcct ctcctatctc
    26041 cgggcctcaa gcccccctca ggatgatatt taagccttga agtcctattg atcctcatgg
    26101 aatgcagcga gccctgccgg gagttctgtc ggtggataga gaccctcccc caccacagga
    26161 agttcgtcct caagaaggag gtgtacccgg cccttccccg gtgcttcaag gagacgctcc
    26221 taggggaggt ggtgccgggg tcggtccggc agctccgggg gccggcgggg tcccacgtcc
    26281 acgagttccc ggaccggtgg gtcctccacc gggacatcgc cgacgccgag gccgaccccc
    26341 tgggccacct cctcagcgac gcccccgagt acctcgtctc ggcgatcgcg gggctagcta
    26401 ccgccctcct cgccaataaa aagagggacg ggaggaacgc cctcctcgcc ggatggtcga
    26461 tgaccgcctt cctcctcctc ctggggaaga tgggaaagac gatcggcgag gacgactccg
    26521 agaaggaggc ccaggcgccc cgcctcaagt cagggttttg aggcggcgga ggagcgggtc
    26581 cggatcttga gctctccccc ccccctgagg ggggcatttt cgatggttta gaggttgagg
    26641 ggtggattcg acgatggtgg agtttgcagg caatttcgct tacttcttct acgctcttgc
    26701 ctcggtcttc atcatagtaa accccgtaga ggcgacgatg gtcttcatct ccctcacccc
    26761 ggaggcctcc gccggggaga gggcgaggat ctctttgcgg gcggcatcgg tcgcctacgt
    26821 catcgccctc ctcttcgccc tctccgggga ccacgtcctc cggttcttcg gggcgaccgt
    26881 cgactccctc cgggtcgccg gagggatcct cctcttcatc atggccatcg agatgctgaa
    26941 ggcgaagccc cggaggaagg tgacggaggc ggagctggcc gacgccagcg accgggagga
    27001 cgtctcggtc ttccccctgg cgacccccct cctgaccggc cccggggcga tcaccaccgt
    27061 catcgtcctg atggggacgg cttcaggccc cgccgagaag ggggcggtcc tcctcgccat
    27121 caccgccacc ttcgccgcca cctacctcat cctccggtcc tcggagtacg tcgaccggat
    27181 cctggggctg acggggatca tggtgatgac gaggatcatg ggcctcctcc tgggggcgat
    27241 cgccgtcaac ttcgtcgcct tcggagcctg gaacatctac gccgggatgg cggggctctc
    27301 ctgagggctc ccgccccgaa aggtggggat acgtcggcga ggatcctcat ctcttcggcc
    27361 agctcagggc ggtccgatgg ctcttgccac cagatcgacg aggtcgatca cctcgacctc
    27421 ggctccgacc ccggccccgg cgtccgagag gtttctgatg cagagggggc agcaggagac
    27481 gatggcatcg acgccagccg gcacgtcctg gagcctgagc tttgcgatct ccagggacag
    27541 ctccggatag ccggcccgga ccccgccccc gccgccgcag caccgggcgt cctcccggct
    27601 cctcttcatc tccacgaggc ggcagatggc ctttatcacc tctcgcgggg ggtcgaagat
    27661 cccggcgtgc cttcccaggt ggcaggggtc gtggtaggtg acggtgaggt cgagccgcct
    27721 tggtctgagg accgagagcc gctcggcgag gaactcgggg acgctcacca cctcgaactc
    27781 cggagggtag tctctcttga gggtcgagag gcagccggcg catccggtga tgatcgtctt
    27841 cgctttcatc tcccggatct ggcggaggtt ctcgtcgatg accttctgcg ccgaggagcc
    27901 ggtcctgatg aggggagagc cgcagcacct ctcctccggg aggagggcga cgccgaacct
    27961 ccggaggata tggaaggtct tggccgccac ctccggatac cggtaggagt cgaggcagcc
    28021 gacgaagtag acggcctcgg ccttctcttt tatggcgccg gcgtccgaga gccaggctag
    28081 ccggtccccg acctcgccga aggtgttgcc gctccccgca accttcgccc tgaacccggc
    28141 ctgggcctcg gtcatgaccc cctgggagac gagccccctc ctcgccgcct ggacgatcgc
    28201 gggggggttt gcccccgcgg ggcatttggc ggcgcagatc ccgcaggtgg tgcaggtgtt
    28261 gaggctgtcg aggacggagg cgtcgggggc gagcccttcg ctgagggctt tagccaccat
    28321 gacccggccc cgggtgttct tcgactccca gcccaggacc tcgaaggcgg ggcagaccga
    28381 ccggcaggtg ccgcaccgga cgcagagctc cagggcccgc gggtctatca tcacctcctc
    28441 ttggtcctgg gtcaactcgt tccccccgga gtcccgtccg cccccgcgcc acgcccattt
    28501 tcccctaccc cggccgggcc gtcgtcgagg ccgagctttc cggggttcat gatgcctcgc
    28561 gggtccaggg cccgttttat ggccctcatc accgatatcg ccccctcgcc cagctgctcc
    28621 gccatgtact ccgaccgggc ggcgccgatc ccgtgctcgg agctgaccgt ccccccgagg
    28681 cggatcgccg cccggtggat ggcgtcggcc gccccggcga gcctcgcctt ctccccctcg
    28741 tccaggacgt cgatgaagag ggcgacgtgg aggtttccgt cgccgatgtg gccgtacttc
    28801 atcgccggga tcccgaaccg gtcggagatc tccgagacca cccttatcag cgccgggatc
    28861 tccttgatgg ggacgccgac gtcctccccg acgtagatcc ggctcttcgc ggggtcgagg
    28921 cgggagaccg ccgcccccac caggcggcgg gccgcccaga tctcctccat ctccccggcc
    28981 ccggcgacga cggccaccga agcggcggag gaggaacaga gggcggcgat ctccgccgcc
    29041 gcctctgcca cggccccctc ggagccgtcc acctcgaaga ggaggacgtc gccgtcgggg
    29101 agcttgaggc cagaatcgca cctcttcaga accgccaccg tcgtccggtc caggatctcg
    29161 caggcggacg ggacgatccc cgcggcgaag gtggcggcga cggcctgccc tgcctcctcc
    29221 gccgaactga aggaggcgat gacgagcctg cgggccttgg gcagtggcgc gacccgcagg
    29281 accgccccgg tgatgacccc cagggtcccc tctgagccga tgaatagcct ggtcagatcg
    29341 tagcccgccg ccgccttgag ggtccgcgag cctgtcctga ccacggagcc gtcggccaac
    29401 accacctcca ggtcgaggac gtaatttcgg gtcgtcccgt acttgacgga cctcatcccg
    29461 gagccgttgt tggcgatgag gccgccgatg gtgcagaagc tcgcgcttcc ggggtccggc
    29521 gggaaaaaaa acccggaggg cttcagcgcc tcgttcagcc gcgcctggac tacccccggc
    29581 tcgacgacga cggctaaatt ggccagatcc atctccaaaa tccggttcat ccccgagagg
    29641 tcgaggacga tcccgccccg gaccgccacc gccccgcccg ctaagccggt accggcgccc
    29701 cgggcggtga tcgggacgcc gtcggcggcg gcaaggctga cgacggcgga gacctccgcg
    29761 gtggagaggg ggcggactac gtaatccggg gtgcccctga cctgggaggc gtcggaggag
    29821 tagcagagcc tctcggcggg ggaggcggag gctctatcgc cgacgatatc gcggagcctg
    29881 tcgatcagat ccatggaaga cctcatttac gggagaattg cggcgccgaa gggcgggcgg
    29941 agtcggagga ggaattccgt cggggggata taatagggtg tcgatgggcg gagcgatcgg
    30001 gttattggca gtaagtaagc agatcccgat cgaaatccat cggaagcttt ttcgggtatt
    30061 gctcatatcg gagccttgga gatgtagagt gtggccgttc cggagaagat ccttgagttg
    30121 gttgaaagat tcgaattggt cattgataaa tataaatcta gttataaaga agaagaaatt
    30181 aaaattgaat ttataaatcc tttttttgaa gctctaggct gggatgtccg aaataagagc
    30241 aataagcccc cagatcagag agaggtcagg tttgaatata ccttgaggtc agagggggag
    30301 acccagaggc ccgattacag cttctggctt gacagggaga ggaagttctt tctggacgcc
    30361 aaaaaacctt cggtgaatat tgaaggagga ataaccccag cgttccagat aaagcgttat
    30421 gcatggtcgg ccaacctgcc gcttgccatc gtaacagact ttgaagagtt tgccgtctac
    30481 gattgcagga ctgagccgtt caaacttgat aagcccaaaa agtcaagatt attattaata
    30541 aaatattatg aatacgaaaa gcgatgggac gagatcgcct cgttgttctc caaagaggcc
    30601 gtccagagag gctctctagg ctccatccct tctccaaaag gaaaaaaagt cgacgacgct
    30661 ctcctggagg acatctctga ttggcgaaat gctctggcgg cgagcatcac caagatgaat
    30721 ccagagatct ctcagaagag cctgaactat gccgtccaga tgacgatcga caggattcta
    30781 tttctcagga tctgtgagga tcggtctatt gaggacagcg agcggttgaa ggggcttctt
    30841 gatggggatg atgtatatga gaggctgttt gaactcttcg aagaggccga taagaggtac
    30901 aattcggggc tgttccactt ccatccagag gaggagagga acaacttcga cgccatcacc
    30961 ccgtcgataa agatcgagga caatgttctt aagaatataa ttcaaagctt atactatccc
    31021 gacagcccct acgtcttctc tgagatccct gccgatattc ttggtcacgt ttacgagcag
    31081 ttcctgggga aggtcatcca gctggacgag agccacaaag tgagcgtggt ggagaagcct
    31141 gaggtgagga aggcaggagg cgtttattat actccatcat atattgtaga ttatatcgtt
    31201 aaaaacaccg tcggcaagct cttggagggg aagacgccca aggaggcctc tgaccttcgc
    31261 atcctcgatc ccgcctgcgg ttcggggtcg tttctgatag tggcctatca gcacctcttg
    31321 gattggcacc tcgactggta caaggcccat ctcgtccctc tcttggatgc tggagagaag
    31381 ccgacatcca acggagtgca aaatctcctc ccgtctccga tcgagaggcc ggggaagaac
    31441 gccaggtccc gatcgaagaa gatctgggag agggcttcgg tggcgaccct ccccatcgag
    31501 gagagggcgg aagagtggca tctgaagacc gccgagagga agaggatcct cctcaacaac
    31561 atctatggcg tggatatcga cactcaggcc gtggaggtga caaagctctc cctcctcctc
    31621 aaggtcttgg aaggagagaa taaggccacc atcaccgatc tcaatatgta ttcagatgag
    31681 agggcgcttc cggatctagg gaataatata aaatgtggca attccctcat cggttgggat
    31741 atccttgaag ataggcctgg tctctcccag gaagagctgg agaggataaa tccttttatg
    31801 tgggaggatg aatttaacga tgtcttcgcc aaaggcggtt ttgatgcggt aattggaaat
    31861 ccgccgtata ttagaattca aatgatgaag gaatgggctc ctgtagaggt ggaattttac
    31921 aaagagcatt acaaggctgc caagaaaggg aattatgaca tttacgtcgt attcgtcgaa
    31981 aaggggctgg gccttctgaa tgactacggt cttctgggct taatcctgcc ccacaagttc
    32041 ttcaatgcga aatacgggca gccgttgcgc agtttgatag ctaaaggtca gaatctaaag
    32101 aagatcgtcc actttggtca cgagcaggtt ttcgatgggg caactactta cacctgtttg
    32161 tttttcctca taggttctca caataatcaa ctagaatatg tgattgttga tgatttagtc
    32221 gcatggcgta gatacgattc gagcttaagt ggtatgatac cagcagagaa gataacaggc
    32281 agtgaatgga acttcgttgt aggccaaggt gttgagctat ttgagaaact aagcaaaatg
    32341 ccaataagct tgggagatat agccaacata tttgtcggtc ttcagacaag tgcagatact
    32401 gtatttctgt ttaaagactc tcgaaagtcg gatgctttaa caaccacggt ttattcaaaa
    32461 cagctcgcta aaaccgttga gttagaatcg gatttactta agtccgttat aagaagcggt
    32521 gatattggtc gctattgggc aaatccaact gctctggttt tattccctta tgaatcaaat
    32581 gggacaaaat ctcaactaat tcatataaat gaaatgaaat gtggatatcc caacgcctgg
    32641 gattatttgg tttcaaacaa actgctttta tccagccgtg aacatggtaa attcaaagat
    32701 actggctggt atcagttata tcccaaaaac ttagatctat gggagcagcc caaaattatg
    32761 gtaccatata tgatcacacg actgtcagca ttttatgaca aaggacttaa ctattttgtt
    32821 aatgtgacaa cgggtggatt tgggattacc atcgaccaac aatatggctc aatgaaatac
    32881 ataaccgggc ttctaaatag caatctgctt gactggttct taaagaacgt aagtaccaat
    32941 tttcgcggtg gttacatcgc agccaataaa cagtttttag ttcaactccc tatcagaacc
    33001 atcgacttct ccgaccccca ggacgtcgcc cgccacgata agatggtgag cctcgtggag
    33061 acgatgctcg acctgcataa gcagctccat gaagtcggca ctccccatga aaaaacgcgc
    33121 atacaaaatc agattgatta tacggacaag cagatagatg ccttggtcta tgagctgtac
    33181 gacctcaccg aggaggagat cgccatcgta gaagagagcg caaaatagcg ttcctgcact
    33241 cattttgatt tataagctcc ggtgccagcc acgccgcaag acccttcttt cacagcaccc
    33301 tccccaccac cggaatcctc ctcagcacca tctcgctcgc caaaaagcat atcggcaccg
    33361 ccaccgccgc cgccaccgcg aacttggcca gggggtgcag cgcgaacccc cgcagggcgg
    33421 cggtgaccag gatcaggatc accgggtgga agaagtagac ggcgaagctg ttctcggcca
    33481 tgaaccgggg aagcctcccg ccggagttga aacgctcccg gaatatgacg acgatcccga
    33541 gaaccgcccc cacgcagaag aaggcctccc agagggcgta ggccgcgctc tgccagtaga
    33601 ggccgccatt gatcatcgat atgtcgccct cgggcccgat cccgactagg aagatggcga
    33661 gccagaaggc ggtcccgccg acgagggcgg ctttgagcca gacgatcccg aagggatagg
    33721 ggatgcggag gagccagcgg cgccggcggg cggcgatccc cacggcgaag agggcgacgt
    33781 aggaggcgaa gaaggagagc tgcatgttca ggacggcggt cccgatcggc tggaccaggc
    33841 ggatcgcgaa ggcggcggcc gcgatgacga ggatgagagc gacgacgtcg cggtggcggg
    33901 ggagcgtcgc ctcctctccc gtcccttctg ctccttctac cccttcggct ccttccgcct
    33961 tctcctcgtc cttcttctcc ggatctcccc gaaggagcct cgccgcggcg taggctgcgg
    34021 agaagatgag gagggcgatg gcaaaccaca tcggccccga gccgccgagg aactggaggc
    34081 cgaggaggta tccgaggtaa aaatctccca gaggggtcag gggtgcctcc gccgagaggc
    34141 gcatcacgta gtatagaatc gccgggttga ttatgagcat gtagatcagg gccgggaccc
    34201 cgaggcggac cgcccggtcc cggaggaacc tggctccgcc tttgcggcgg aaggaggcgg
    34261 ggacgaagta gccggcgacg aggaagagga ggcccaggga gtaggcctgg atgaaggact
    34321 ggatgatccc gaaggagagg gtggtgaggg ggccaagggc cgctagctct cggtagtacc
    34381 aggaccccag gcccgagtag gtgacggcgg cgtggaggac gacgacctgg actatgacga
    34441 aggagcggat gttgtcgacg aagtagaggc ggcctttctc tgccgcacct tccggcatga
    34501 ccatccttaa gctcacctca ccctcacgct ccggaatctt ctattatttt tcaaatctag
    34561 gtttaaattg agtccagcta ttttaacctg atccgccctc catcgaagcc cgccacccct
    34621 ccctcctcct cctcccctca aagctcccgg ccgtccaccc cgaaatcgcc agggatcatc
    34681 cggcagagag atgatagagc ttgaacggtg gggcccaatg gtgccctatc agacccggcc
    34741 gcccttctcc tcgacggcga actcctttat gtcgaggaac cggagcatcc cccgagagac
    34801 cgcctccgcc tcctcgattc tgagcctctt ttcgaagatg gggccgtcga ggactagggt
    34861 gtgaaactcg ccattctcgc cgcaggggtc gattgatggg tcgagggctt tcagctccgg
    34921 gacgaagctt ccatcgatcc ggcggccgag ccactcgtcg ccgaggaggt cggaccggac
    34981 cgcgacgatc gccgcctcaa agccggaggc gagaagctcc ccgatgacct cttctgagtc
    35041 catcatccag atcggcatca taggctctat gccgaggtcg gcagagatcc gcaggacgag
    35101 ggcgtcgtgg gtctttatgt ggccgaagat ggcggtcgcg atcctccttc cccggcccct
    35161 ctcggcctcg tcgagctggc gaacgacccg cttgaactcc tcctcgtacg agaagaagcc
    35221 ggtccggatc agggatagcc ccatcgattc cgcctgggcg gcgaggaggg cggccttcac
    35281 ctcgtgggcc ccctcccggc ccatattcca gaaggagagg aggtaaccca ccttcgtccc
    35341 cgcctccatc gccctgtagg cggcaaggca cccgtccttc cccccggtcc aggagacgac
    35401 ggcgatggcc cctcccgccg ggtcggcctc accccccgga tccctcctcg tcttcaccct
    35461 cctcccccca gggtggagac tatcctccgg tcgagggccc cggcctcctc ccttatccct
    35521 tcatcctccg aggcgatcag cttcgccaga tcccgccgga ggggcccgat ccggaacgcc
    35581 tcctcctccg ccggcagccc ccgggccgtc gagaccgcct cctccatccg ggcgagcctc
    35641 ctcctttggg ggtcgtcctg gagggcgtcg atgagccccg acaacgccgc cgccgccctc
    35701 tccccccggg gcgtcagggc gagcctccgg atcctcccca ccggctccga ggtgacgagg
    35761 cccatcccct ccatggcggc caggaccttc agggtgtggg ggacggtgga gtcgatcgcc
    35821 ttcgcgacgg cggagacgta ggcggggtcc agctcccgga tggcgaggag ggccatcgct
    35881 ggcttatccc tcaagaagaa gagctcaggc ccccccttca tcccctcccc tctcctccgc
    35941 cgcccttcac cacccctcgg aggccgtatc cggaggaggc ctcagggctc agggaagccc
    36001 ccctccggga ggtcgggggg gaggacgggg gcggacctcg cctcctcggg gaggaggacc
    36061 accaccggct cgccatagcc cccaaaccgc ctcacctcca gggtttcgat cacgatctcc
    36121 atcccgccgg ccgcctccgg gggcaccctc aggatccccg gcccggcgga atagacggca
    36181 gagatccggc tctccaccgg gagccccgtc tcctccgaga cgaggacggt ccattccagc
    36241 tccgcccccc tctcaaagag gcggtccatc tcgatcaaga gggggattct ggggtcgatg
    36301 gagctgatct gccccagcat gatcccgaag gcggtgtcct cgtcgggggc gaccttgagg
    36361 ctccaaaccc tctcgccgtc gacccggtcc gtcccctcaa cggccacctc gccccgatcg
    36421 atcatctcca tcatatgggc gagctgatcg gtcttctcca tcctgaaggc cgggtccggc
    36481 tccaggaggc cgatccattc ccctcccatc ctctggtatc gggtgtcctt caggaagtag
    36541 acctcgacgg catcgtcggc ggtccggtcc ccggcgaggg tcgtcctcct cgactcgatc
    36601 tcctccatcg agctgtagtc ggtgaggttg atctcccccc tccaccggag ggagatcctc
    36661 tccccctcca gggacccctc tccgctgaag ttccggtact cgacggtctg gtccatctcg
    36721 atctcgaacc gataggattc gagatctccg accgcctccg ccatcatccc cctcacctcc
    36781 tcctccgtcg gcgcaggctc cctctcgacg catccggcga aggccaccgc tgccagggcc
    36841 accaagagca agatcctctt caccaaaatc cccctctccc cgtccatcgg ccccccgccc
    36901 ctcggccctc cgggccggag gagggcgacc tccccggtct ggggcaaagc ctcacgagcg
    36961 gcccttcctc atccggagct ctcaaacttc tccccggtga tcatctcgaa gagcctgatg
    37021 tatatctcgc tcgtggtggc gacgacctcc tcggggaggg gcggtatatc gggctccgcc
    37081 tctcccttat cccgggcccc gtagagccgg tccttgtatc cggtcctccg gtagtgctgg
    37141 cggacgtgct ccttggagag gtcgacgaac tccccctcct gatacttcgc cctgtcccag
    37201 aaccggtcct cgtcggcggt cccgaagacg tcgatcacca aaagctccct atcaccatcg
    37261 aaggcgaact ccttcttccc atcgacgtgg atgagccccc ggggctccac ctctcttctt
    37321 atatcctcgt cgatcctgag gaccgtctcg cagatggcct cgtactcctg gggggtgatg
    37381 ccggcgagct tcagcgcctc gccccggtcg aggagcctgt ccaccctctc cagcttcgtc
    37441 gatacctcga tgaacggatc ggggaggggc tcgccgtact cgaccttatg ccccccgggg
    37501 aatcccagct cctcggggcc gaccttcccg gcgacgagcc tgtcgaagat cgatcccgcc
    37561 gcgtaccacc ggcagatcac ctccaggggg atcaggtagt tcctggaatc cggcctgata
    37621 tcctcaggct ggatcacgtc gaccttcttg accagcatcc cgttgggggg gagaaactcc
    37681 tggaagtggg tctttatccc catcctcttc gcccggttga accagtggac cccctcccgg
    37741 cagagggtct cccccttgtg ggggatgagg gtcgggatca ccttgtcgaa gacggatatc
    37801 ctgtccgtga acctgaactc cagctcccgg ccgaggtcgt agacctcctt gaccgagccc
    37861 ttcctcagag gcttcctttc catcgtcccc ccatctcctg gcagatggat aactcttttg
    37921 ctgaacggag gtccggcgga gttcttcgca aggtgaagat cttcagatcg agatgtctaa
    37981 ggccacgccc ccagcccgga ggccggtccc cgggatgatg ggggcggcag gacccctccg
    38041 gggtggaggg gggaggaagc cccgccgggg gggccggccc aggagccccg ggcttcgccg
    38101 gttccaagaa gcgcccccgg cgcatagatg gcgtaccctc ccccagccgg gctcgtcgag
    38161 aggccgatcc agagcctgac gaggagcggc tctcccagga ctacgcaccg gaggtccagc
    38221 tggttatagg tcacctcccc ggcggcggcg acctgctgga ggcctacgcc cgtggtcatc
    38281 gtcccctcgc cgaagatctg gccttcggcc tggtagaggg tgaggtccag cctccggggc
    38341 gcctccccca ccagggtgac cgtccagacc cctgcggctt cggaggccct cggcccgggc
    38401 gccggccagg gggggaccct gtcagcggat aggttcatga tcccgaggag gtagccctcg
    38461 gtatggtgga cgtcggcgat cctcagccct tcgatcccga gccattcgtc cccgtagtat
    38521 ccgtaagccg acgccggaag ggaggcggcg aagatcgcgg ccgtcaaagc cagaagaatt
    38581 ttcatatcac aaaaccctcc taaaagtccg gggaaaaaaa gattatattg gcctccggtc
    38641 aggccatggc gtttccgccc gataccccca tccctactct gaccgtaccg gaggagtccc
    38701 cgggggcggt cagggaggtg gggtagaagc ggctcccatt gcagtttccg gcccaggtga
    38761 cgccgtagga tgagtaggcg gtgtaggtgc cgaagatgct gtttccgctc accatcgtcc
    38821 ggagcctgta catgttgtct ccgccgacgg tgacgaggcg gagggacagg gaggaccctt
    38881 ccaccgaccc cgccgccgtc acatcctgga agctgacccc cgtggtcagg acccccctac
    38941 cgaagacctc gtttccgatc tggtagaggg tgaggtctgc gctcctcgat tcggtgtcga
    39001 cgaggttgag ggtccacctt ccggcgaccg cagcccaggg cgatcctgag gtcgtcgtcg
    39061 gcggggtgta tacattgcct ccgccggcgg caccggggct catcccggtg acgggatcga
    39121 tgtaaaagag ggactcgttc ctgaaggaat ccctgatatc cgcccggagc tggcttccag
    39181 tcaggtcgtc caggttcggg ccgtcggccg cccctggatg ggctagggct gccagggcga
    39241 tcacggtcaa agctatttga agtctcattt cgggtaaacc tcctttatcc taatggtgat
    39301 ggatggggcc gggccccgga cccggcctca ggttggctca agcccttcga gatccggaga
    39361 ccgtccccga ccagttggac ccgtcggtcc cgtaagcgga gtagctcccg gagacgaggt
    39421 ttccggtgag ggcgaggtcg aagcggtata gagagaccga atcgagggag acgaggtcta
    39481 ggacgagcct atctccaacg acggttccgc tggccgccgc ctggctcgcg gatgagagcc
    39541 ccgagggggg ctggttcagc caatcgatca tcgactcgat cccccggtcc cccgtggagg
    39601 agctgacggc ctccgatccg ctcgcaggtc ctaagacccc cttaccgaag acgaggtccc
    39661 ccgtctggtg gagggcgagg tcgagggtcc tggtggtgct ctccgtcacc gccagggacc
    39721 atctccccga gaagttggag ggctcctccg cctccacctc cgccggcgct gtcgtcccgg
    39781 tggaggtgcc gatggcgtac acctcccttc gggcccccgg ggtcggcagg gtggctatcc
    39841 ctccgtcggt gggcctcagg gtcgtattat tcgccgggtt gctcccggcc ttggccaggt
    39901 actccgcggt gttgttcacg tcggctatgc tcatgctcca ccctccgagc cagtcctgga
    39961 gggcctggcc agcggagagg atcgatatga agatgacgag agatagcaga gctctcttgc
    40021 cgtttatggt caaatcttaa cccccgttcc ctttaggtga acttcagggt ataaaatcat
    40081 ttggtcatca aaaaccgctt tcggtgaggg tcatctctcc ggatcgaaga tctcaggatg
    40141 ggcccgcctg aactcctcat ccttcatcat gcaccccgtc ggccttctcc ggagccagcg
    40201 gtcccggccc atatccctcc agatatcccg gaggcttcta tcccggacgt tcccgaaggg
    40261 ggtgggcatg taggcgcagg ggaggacgtc cccggaggcg tcgacgtgga gccacctcct
    40321 ccccgcaaag cagccgaaca tctcggggcc gaggaggtag gggaaggccg agacccgggg
    40381 cccatcgggc aggcggttct tatcgaggtg gaaccggccg agccgctcga catccgccgc
    40441 cgtcaggacc tcgtcggcgt gggaggacca cctccccacc gccacgatct cgtagaggga
    40501 gagctcctgg accgagagct ccgctgccag ggagtaggcc tcctccagct ggtcgatgtt
    40561 cagcggagag gcgaccatga agaggtcgac catcagcccc gcatccaggg cgtttcttat
    40621 cccggtgagg gcgtcggaga aggccccctc ccggccgcgg aaccggtcgt ggtcctcggg
    40681 gcgggggctg tcgatggaga tcctgaccgc gtagagcccc gcctccttca gggccctcgc
    40741 ccgctcctcg gtgaggccga agccgcttga aaaggtggtg gtgatcgccc tctcgtcgac
    40801 tccggccacc agctccggga ggtcccgcct cagcatcggc tcgccgccgt cgaaggtgat
    40861 caggtaggag ccgagctcca gggcctcacc gatcgccctc gatatatccc cggaggcgag
    40921 gacctcccct ccccgggagg gggcgctgca gtggaggcag tcgttggggc aggcgcggac
    40981 gatcccgatg gagaactggt cagggacctt tcgccgggtc agggccgcca cctgggagga
    41041 gatcatcctg tcgaaggcga ggctgggagc gggggggatc caggtggaga agacgattcc
    41101 ctcggggccg gagctgatcg gcttctcgcc gtcgaaggtc tcgttgatct tcctcatgac
    41161 cgggcccgcg acccgggaga gggggcccgt cgcccggaag gcgatcttcc ccccctcggt
    41221 ctcggcctct atcttcaaca gcggcctttt tgcgacttgc attcctctcc ccacttgtct
    41281 acgatgcttc cgagcctcgc cttgacctcc tcgtctcccc ccgcctcggc gagggccccc
    41341 ttcgcggcgc ggcagtgctc ccggatcgcc tcggcgcaga ggcggaccgt cgcctccgcc
    41401 gagtggcccc tccggtatat caccggaagg gtggcggagg tcttagccga agactttccc
    41461 ttctccatcc ccagaaactc gttcaggtcg tcggcgatct ggtaggcgag gcccaggtgt
    41521 ctcccgtacc tctcgaagag ggcgacgtct tcggcgccgg cccccgctat catgcaaccg
    41581 atcttggagg aggcggagat gagcgacgcc gtcttcttgc atatgcagat gtagtagtcc
    41641 tcgggggtcg tcctgctggc gaggtcgagc atctccccct ccgacatctc gacgcaggcc
    41701 tgggagaagg cctcgatcac cagctggcgg tagccggaga tgaggccgat ggacctggat
    41761 atcaggtagt cgccgcagag gagggcggcc tccatcccgt acctcttaaa ggcgctctcg
    41821 gctccccgcc gctcggtccc cccgtcgagg atgtcgtcgt ggacgaggga ggcggcgtgg
    41881 acgag
//
LOCUS       DASM01000067            5140 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_63193, whole genome shotgun sequence.
ACCESSION   DASM01000067 DASM01000000
VERSION     DASM01000067.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_63193"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1118 a         1535 c         1626 g          861 t
ORIGIN      
        1 cccaccacct cagcggcggc cagaaaaaga gggcggcgat agccggggtg atggcgatgg
       61 agccggaggt gatgatcctc gacgagcccc tctccaacct cgacccggcg gggtcggaag
      121 aggtgatgga gcttctggag gagctgaacc agagggggac gacgatcatc atatccaccc
      181 acgacgtaga cctcgcctac agctggtccg atcggatcta cctcctcgcc gacgggaggg
      241 tggtggacga ggggcggccc gaggatatct tcggagatct gaggctcctc accggctcga
      301 ggctccggca gcccctcatc ctggaggtct acgacgagat ctcgaagagg gaccttgctg
      361 cctccggaaa gaggccgagg tcggtcccgg agctcgtcgg ccacctcagc ccgccgaacc
      421 tccgctggat gagggtcccc cccgggacca aggtgggcga gaccgtctct cccgacggga
      481 ccggaagcgg aggatcgggg ggggagagga ccggtgatat gagggtgatc catatcgagg
      541 acgggagagc cgtggtggag cccctagata gaccgataag gatcggccgg gtggccgtat
      601 acgacaccca ggtaggagac cccagagacc tctccagcct cctccatggc ggcacgatag
      661 acctcgtggg ggtgatgggg acgaagagca gggccttcgc cgaggatagg gggatcgttc
      721 cagacatcgg atcgaacgtc atagacagga cggtcctccg ggccctctgc ggagaggcgg
      781 tcctcatcct caccaacggc gggatggtgg agcacgcccg gcggcggatc gaggagtact
      841 gccgccagag cgggatgaag atcgaggtct ccgtcgtcgg cagagaggag gaccgggcgg
      901 tggagaagcc ggtgcccgcc cccgccgacc cagggaaggg cggctgaggg gatctccccc
      961 cctcgagaga tcatcaccgg aggcggagat taattcttga tagcaatatt attcataaga
     1021 tatcggtcgc tgattattct atcaagccca attgataaat aacacaatat ggagaaccaa
     1081 cgaacccatc cagccaccat agaaacatcg agacataaat aggtgtgagc gggtagggga
     1141 gtgggggtta gtgaaaaatt agtttacccg ctcgtgttta tacctctgta tgatcatata
     1201 aatagttttc cggccaataa ttcaaaacag ccattacata tttatcctat tcgccacttg
     1261 gttcgcgatc ttcgccgcaa aagcgccagc cacaaacccg gcgtcgatgt tgaccacgac
     1321 catcaccgag caggactgga gcatcgagag gagggcggcc tcgcccccgc ccccggcacc
     1381 gtacccggtc gagacgggga gacccaccac aggagcctcc atcagccccg ccaccaccgc
     1441 ggggagggtc ccctcccggc ctgcggccac caccagggcg tccatatcac gcatcttctc
     1501 cagggagggg aagagcctgt ggatccccgc gacgcccacg tcgtactcga cctccgtctt
     1561 gcagcccatc atctcggcgg taaccctcgc ctcctcggcg acggggatgt cggcggtccc
     1621 cgcggagagg atccccacct tcccccccga gggctcaggc ggccaaccct tcgagataac
     1681 cgccgccttc gccctcgcct cctcccggag ctccgcaccg gcggggaggg gggcctccct
     1741 cagggccgcg agctggtcgg gggataccct ggtgatgagg accgccctcg aagcctccag
     1801 gaaggagagg gctatcgcca ccaggtctga ggggcccttc ccctccgcca ggatcgcctc
     1861 tggaaccccg attctgtcta cccgccgggg atcgagcttc gccatctctc ccacctcccg
     1921 ataggagagg aggttcacct ccctggcggc ctcatccaac gagatctcgc cccgggcgaa
     1981 cctctccagg accgctctct ggtttctagt caacagatgc accttctctt ttggggataa
     2041 atggtttatc ctgggccgtt cgatctgaag ggtgatgaag atcaacctcg atctccacat
     2101 tcactcccgg tactccgccg ccacctccgg caatatggac cttcccacca tcgggaggga
     2161 ggcggcgaag aaagggatcg gggtcgtggc caccggggac tgcctccatc ctaggtggat
     2221 ggcggaggtg agggagctcc ccgaagaggg agggctcttt cggctcggag ggacggcctt
     2281 cgtcctcacc gcggaggtgg aggactcggc gaaggtccac cacctcatca tcgtccccga
     2341 cctctccaag gcctcggagc tggcggaggc cttctcttgc cggggcggga accttgaatc
     2401 cgacgggagg cccaccctcc ccatggaccc cccggagata gccgacgccg ccctggaggc
     2461 cgactgcctc atcggtccga gccacgcctt caccccctgg accggtatgt acgcccacta
     2521 tccctccctc gcagaatgct accaggacca atccgatcgg ataaagtacg tcgagctggg
     2581 gctgagcgcc gactccgact acgccgaccg gatagccgag ctgagggtga tcaccttcct
     2641 caccaactcc gacgcccact cgccgtggcc ggataagctc ggaagggagt tcaaccagat
     2701 ggagatgaag gacctctcct tcgaggacct gaggagggcg atcgggcggg aggaggggct
     2761 ccgacccacc ctcaacgtcg gctttccccc cgaagaggga aagtacaaca ggaccgcttg
     2821 cactcgatgc tatcggcatt actcccccgg ggagatggcc gagaggaggg ggaggtgcga
     2881 gtgcggtggg ctgataaagc tgggagtgag ggatagggtc gagatgctcg ccgactttcc
     2941 cgagccgatc catcccgacc accgcccccc ctacatccat ctgattcccc tcgccgagat
     3001 catcgccatg gccctggggg tgaagagcgt ccattcggcg agggtccaga gaacctggga
     3061 ggagctgatc cggggaggaa gcgagatcga ggttcttctg gaggccgacc tctcagagct
     3121 tgctggggag gagagggtgg tggaagccat ctccgccttc aggtccggag aggtggtgat
     3181 gaagcccggt ggcggtggaa agtacgggac gatcgccctt cgggccgccc gggaggaggg
     3241 ggtgagaggg gagaggatgg gggagaagag gggcgaggag cagaggtcga tcttcgattt
     3301 ttagaggggg aagggggtcg catctcatcg ttccgccggg gagagaagga gactataccg
     3361 acgagatcgt cggcgggatc cagagccgat gggatcgatc ttcaggaggg ggggggctga
     3421 gcttcggggt gacggagtac gagaccctcc cccccggggg ctcctccaga aaatgttaag
     3481 tactttgtgg gggaatataa aagggtatat accgaagggt agaagagatc tctttaaaag
     3541 gcatgtacag atgaagcttc agggctgaac gttagagaac cgatcgtctc cggctcgaga
     3601 agggcgggcc tggagccaga agggaagccc tggaagcttc agtcgaaggg gggagagata
     3661 tctcatatga aagggagtag cagctcagct ctaactcata ggctcaccgt tgcgttcctc
     3721 atcctgctga tccatgcagc caccggtatc gccgatgagc aggtcgtttg cgagaagccg
     3781 gccggtaagt ctccttcgga gcctttgccg acggacgaag gcgtcctgtc tcaagaggag
     3841 gaggcgccct ctccagggcg gaccgacgat ggcggccgat ggatggtacg ccagacgcct
     3901 cctcgaaccg atgacaaaga agaccaggtg agcacggagc caccaaaatt ttgcctatcg
     3961 ggccgcaagt actgggataa aaaccagaac ggagagcgag atcccgagga gggggatctc
     4021 agcggctgga cgatcaccgt tgatggggag tccaccacct ggtggacggt caccaacatc
     4081 cacgggtact ggaagatctg caacctgtcc gccggcacct actacatatc cgagcgcccg
     4141 cccaagggag agatcggctg gatctcatcc cggaggcccg ccaaggtcac cataaccgat
     4201 aggtcgataa aggacctcaa ctccggaaac taccgcgccc tccacgacga gccccgccag
     4261 tacgagcagt tctgcgagag tcagctggtc accggatcgg ggtatctcga cgtcaagaac
     4321 tcggtcctgg acaagaagct ggcgatggac tatcagaaca agatcttcgg ggagggggat
     4381 ataggcctcg agtcggccca ggtctattca caggagccca acaagctcag aaggccaata
     4441 ccgaactgca gcgaccccaa tggaaccacc atgcagaggc tcaacttctt cgataatacg
     4501 aaggtcgtct acgagggcga gacccctctc gtcggcagca ggtcgatcag atccatagaa
     4561 cgttacgggg ggatcggcgc cgatatccac gagaacttca gcgtccgacg aatggaggcc
     4621 gaccagacgg tctttctggg gacgacgagc aactccacca tcaggcggac ggtgggatcg
     4681 aatacccaaa acagctttga agggatgtgg gggacagatt cgagactcca caagatcttc
     4741 tacaaggata tcaagagacg agaaaggttt tcgggggatt tcgaggtgct gagggagctg
     4801 aagttccacg agaatccggt cgctgagccg gggagatgtc cctgcggcgg agtggactgc
     4861 tgaaggcgag ccggccgtga tagacccata tcctccccca tctttttttc gccatcctcc
     4921 cgcccaatca tcccttcttc aactcttcca gatctagagg tccactcggc ccagcccacc
     4981 agataccata tctgtaatcc cgatatcatc aggcagacgg cccgtgaatg tcccggaaaa
     5041 atgtccgaag ctggcccatt tcacgtcaga ggttagggtc agaataggag agaaaaatgt
     5101 tatgccccgc gccctctcac ggcgcagggc acgaccccct
//
LOCUS       DASM01000068            8083 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_130370, whole genome shotgun sequence.
ACCESSION   DASM01000068 DASM01000000
VERSION     DASM01000068.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_130370"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1539 a         2496 c         2526 g         1520 t
ORIGIN      
        1 ctcctgcatc gcctccagca tggcgctctg ggtcctgggg gaggcccggt tgatctcgtc
       61 ggcgaggacg atgttggcga agatcggccc ctcctcgaac ctgaagtgct tgctcccctc
      121 cacctcctcg atcagggtgg tgccagtgat gtctgcgggc atcaggtccg gggtgcactg
      181 gatcctgctg aagtggaggc cggtggtgct ggctatcgtc ttgacgagga gggtctttcc
      241 taggccaggg ttgctctcca ggaggatgtg ccccccggcc aggatcccca ccagaatctg
      301 ctccaccacc ggcttctggc ccaccaccac tttgccgatc tcttctctga tcgtctcgaa
      361 gatggctggc gcgtccttat agatgttgat cagatcgtct ctcatctctc cgccctcggt
      421 gaggtctaaa gctccttttt tgtatatttt catttattat ttctcgtttc cccctatgca
      481 cctcacgaga tcgtcgccag gttctcgaag tacctcttga tgatctcccg gtgctggggc
      541 gggaggctct cgatgtactg gtccgtcggc accgccaccc cctccccggg ctccgagatg
      601 gtgaagaacc ggtctacggg gtcggtctcc ctagaccgga agccagcggc ggagccggga
      661 tacaagacca gctccaggtt cacctcctcc aggacggcga gggacggctt cccgtagatc
      721 tcccccgaga tccccgggct ggaccccgcc tcctccggca gctcctcatc ggcgaagagg
      781 gccatcgccc tatcccggag gtcggagagg gaggagaagg gctggggcgg cgcaccgtct
      841 cccgggctat gagcgaagac ggccgccgat gcgaggagga ggaggagggc cccggccttc
      901 gcctggaagg cggggacgct cctcgtcccg aggtagaggc ggcggctgtt catcaggtcc
      961 gcggccccga cggtggagag gcacctcttg acatcctcgg ctaggctttg catcaccacc
     1021 gagtcggagc cccggttgtc gtaggccgcc ttcgtcttct ccgagatcgc ctcgtcgagg
     1081 tgggagaaga ggtcgtacct cgacctccgg tcgatgacct ttccccccag aagccccagg
     1141 atgagggaga cgaaggcggg ggcggcggcg aggatggagc cgtgccagta gaacccgaag
     1201 aaggtgggga tccccaggta cagggcgacg atgtagatca gggtggcgat cattccggag
     1261 ttgaggaggt agagcccccc ctcaagccgc ctgtaggccg cagatagccg tctcagatcc
     1321 tccagaaact cgtacgatcc gtccacggag atcccttcag agcccttagt aataagtagt
     1381 ttcctcaggg ggatcgcctt cggagccgga ggtatgcata aagatatgct ccctttcggg
     1441 gaggggagaa ggtgtagatc cagctagtcc tcaggagcgg accctccagg accctccaga
     1501 gccggaagtc ccgggtggcg atctcgtaga ttttgcagtc cctggaggcc ggtgattgac
     1561 gacggtcgaa ttaggtttat agcctttgaa gaacaagacc tacccgtgga ccttcggcct
     1621 gatggccgga ggaagagctc cagaaaggtg ggggataaat tgacgatcga ctcagggcgc
     1681 ggatccagcc ggaagcgact cccgctggat atctgagaga gatgagaggg atttctggga
     1741 tggcgaggct gagctggcag ctcctgacct tgatcctcct agcccagctg atggtgccag
     1801 cggcgatggg cgccgcctcc accatcgccg gggatggggt tcgggtcgga ggccccgacg
     1861 tcaacggtag ctcggcggtg aaggccccgg gggaggcggt ggcagccggg atatggatcg
     1921 ctcccgatgg cgactgggag aaggtcaccg aggatatgaa ggagaccctc cagtccctcg
     1981 ctggcccctc ccgggttctg aggatcatgg tccaggagga ggaggatctg atcgaacaag
     2041 ggtacatcgg aacctccgat ggggtcctgg tcctctggcc cgacgtcacc gagaccctcc
     2101 agctgatcgc ccccttcgat ttccgggaac gtccctggta cctggaggca gcgagggccg
     2161 gggatacgat ctggacgaag ccctaccaga gccagaccac gggggatatc tccatcacct
     2221 gcgccacccc catatcggcg gaggggaacc tgagcggggt ggtggggatg gacgtatccc
     2281 tggcggagat ggacctcgac ctcgcgaact tctcctctgg ctaccccttc cttctggacg
     2341 gccagggggc ggtggtgatg aagccgccaa ccccagtggg cttcctctgg gacgagatcc
     2401 tggagccagg aagcctcctc gactcgaaga acccggagct ggcggatctg gcggaggcga
     2461 tggtccgggg agaggagggg acagccttcg tccggctgga taaggggagc gctaggatcg
     2521 tctacgcccc cctcccctcc gtcggctgga gcctgggggt cgcatccgga gaccaggacc
     2581 tagcggcagc caaggcctcc gcttacgatc agctcttccg ctgcatatcg gggcatacgg
     2641 agatcctggc cctggagacc ggcggggccc tccgagggga gagcttcggg atggggatct
     2701 ccctccggga agggggtccc ggcccatcca ggccgatggc cctaggagcg gccgccatct
     2761 ccctcctgct gggggcgctc ataggatggt gggcggggcg gagggcctcg gatccggtct
     2821 tggaggccgt ggcgaggggg cttgaggagg tcgggagggg ggacctggag gcgaggatcg
     2881 cgggggacgg atcgaggagg gctaggaggc ttgcggaggc cttcgatgag atggccctcg
     2941 gcctcaaaga tcggatagcg aagtcggagg agaggtccct cgcagcggga aggtctgaga
     3001 aggcggggga ggtgatcgcc gaggtccaga ggttcctcat cccggagagg gttccccaga
     3061 tcgaggggtt cgaggtggcg atcctggccc tggccgaagg tagggagtgc tgccacttct
     3121 acgacggctt cgagatggag ggacggaagg cgatggtcgt tctggccgag gtctcgggga
     3181 aggggctctc cgccgccatg gcggcggccg tcaccagaag ccacatcagg gcggctgccc
     3241 ggaggcttgg agatccggcg aaggccctca gagagacgaa ccagaacctg gtcgggaacg
     3301 tcagaaacgg gatggtggta tcctgcttct gcgggatcct cgacctctcc agccacgccc
     3361 tgagctacgc caacgccggc cacgtccccc ccttcatcgt cagctccgac ggcttcgtcg
     3421 acaccctcgt cggcggcgcg atagccatgg gagccctcga ccacatcgac ctggagatgg
     3481 agggttggat gatagacccc ggggacgtcc tcgtcatcta caacgacggc ctcatcgagg
     3541 tggaggacgg ggacggggag aggttcggga ccgagagcct gataaccctg gtgaaggaga
     3601 accgggagaa gccggctcag cagatagcaa aggacctgga gaggaggatc gaggatcaca
     3661 tgggtggcgt cagagggagg cccgatgccg tcgccgtgat agtaaagagg tccggatgag
     3721 ccgccccgga ctcctggatc gatcggttca taaccggcgg agggaccgga gatcctcgcc
     3781 ggccgccctc ttcacgaggt cgaccagatc ctcgttgtcc agcccctcca tcgagatccg
     3841 atctcctccg ccgaagacct cctcccgcac ccgtcggccc accaggtcgt cggagccggg
     3901 acggaccctg aggatatagc tccctttcgg cgccgccgtc ctggggcaga gcatgtcgag
     3961 gtcgccgtcg acgatcccca ccaggggtat tccgaggcgg gcgaggacct ccccggcgat
     4021 ggcggtggtg tcgtcgccga cggtcaccgc cacccccgcc ccccgggccg cctcgaaggt
     4081 gccttcggcg gcgtgatcga tgaggacggc gtcgcggccg ggttcgcgga ccctcctcgc
     4141 agaagcctcc gtccgcctca catccccggt cctgaggatc gcggcctcca ggtcgacgga
     4201 tgggatcttc tcgaggccgt gctccttcac ctcagccccc aggacctgaa cgattctgcc
     4261 gtcggtagcg gatatcgtca ccctctcctc ttcggccctg ccgatgacga tcccgttgac
     4321 ggagacgttc tcgccgggga cggccccgaa gatcgtcctg gtgatggtcc ggcccgagga
     4381 tgcagtcatc cccggaggtt gaggggccgc caccatcggg aaccccaggg ccccagcgat
     4441 cctctcagcg atccccccct ccccgccggc caggacggag gcgaaagggc cgctatactc
     4501 cgccaggatt aggggtttat ggggcttcgc cctctcagcc accatcgccc cgaaggcgag
     4561 gcccgtctcc gcggtcttgg catagttcaa gaggactacg acgtcgacgg aggacataag
     4621 ccgctggacc gaccggctcg gggcctcccg cctcgatatg tcgatcctat cctccagggc
     4681 ggcgtcgatg acggcgaccc tccccatcgt ccccccgagg acggcggtga ccgatcctat
     4741 cctctcgagc cggtccaggg cctccctggc ccggccggag tcgacgacct cggggccgtg
     4801 gatgacgatc ccaatctcat ccgccgctct cgacgctctc atcctccatc ttcaccgacc
     4861 tcatcctctc ctcttccgcc ttcatcctct cctccatctc cgccatcgtc cggccgccga
     4921 tacccctcct ctcgggcatc ttatcgatcg gctgcagcgt gctcaggtgg tagtagagcc
     4981 ccgtagaatc gaggagcgcc cacctttcgc cgtcctcttc ggtcttcagg accttcacga
     5041 tccccaccgt cccggtcccc gcgtacctga cgatgctccc ctcgctgatc tcctctgcca
     5101 tgatcctgca tcacctcaca gaagcttctc cttcaccacc tcgcccgcga agttcttaac
     5161 ctctgccccc ccggggacgg agaggacctc gaccccgtga agctcgtcgg tcccgcagag
     5221 gacgtcctgc tcggggtggg tgcccgcctc gatatcgcac cttatcctcg tcctctcccc
     5281 cactgagacg acggtcttca gcctcgggga ggcgggatgg cctctgggga ggactatcag
     5341 gggagagttc atctcccgga tcagaaatag ggtgcagagg aggcccctct tcgcccggtc
     5401 cccgaccccg gaggtcgagg cgacgaggag gtcgtcatcc tcggtgaaga tgacgatctc
     5461 ctcctcatcg gtctcgagat aaaaaaagcc gccgagggtc ctggccacgg ccagggtccc
     5521 ggtccgcccg gtgagatagg cgatctcgtc gtcttccagg gagatcttca ttccatcact
     5581 cagcaggctt cacgttcctc gactggcacg aggggcatct gaccctcctg cccatcccct
     5641 tgaacttgtt tccgcagtcc tggcatacgt agcccttcga cgctatcgac tcgacgtcgc
     5701 ctatctctgg tccgcctcca acggtacaca tggggctttc acctccatcc atcctatttc
     5761 caatggctct tatttaaaag aggtggcttg gagcatcaga ttcatcttcg tagcagcgcg
     5821 gagaccccgg tctttaggcc ggggaggagc gcgtgcctac acctccgaaa agcatatatt
     5881 atctaacaac ctgatatatg aatgtgctta gaacggttac cttcaagctt aggatgggcg
     5941 accgagaccg agttactctc gctgaaacga tgagagctta taccaaggct ttttccgaat
     6001 ccgctaaatg gggatttggg aacaaagtcg ataaccatca tgccacctat aagcttgtcc
     6061 gtctaagcat tcctgatctt ccttcgtctt tggtacaggg tgcaagagat tgcgcgtgtg
     6121 aagctctgaa agcgtgcaag tgcagatctc ttccagagcg aagaccgctc gctgcgatga
     6181 ggtacaacca acgagttgtt acagtgaacc tcgttcacgg catagctacg attgcgtcca
     6241 tcaaggggcg aatcaaggca acgttcttta taccccagaa ctaccgagat tatctcactt
     6301 ggaagatcaa atcgtccacg atatcctatg atcgacgatc cgatacattc tatctacacg
     6361 tgtctcttga acacgacgac ccccaaccca taactgagtt caaggtgctt gggatcgatc
     6421 gaggtatcgt gaatattgca gtctgttccg ataacacgtt ttacaactcg aagaaggtta
     6481 aaaataccag agctaagtat gctcaccttc ggaaagagtt gcagtctaaa ggcaccaagt
     6541 cggcaaaacg caagctcaag cgtctcgccg gtaaggaaag acggtttgtg actgacgtga
     6601 accactgtat atcgaaacag atcgtgaact cggattatac ggtatttgct cttgaagact
     6661 tgagcaagat ccgagtccag aagcgaagag gtaagcagtt caaccacaag ctcaacaatt
     6721 ggtcgtttta ccaattggaa cagtttatca ggtacaaagc ggaagctatt gagaagcata
     6781 cgcttctcgt agacgccagg tacacctcgc agaagtgctc tgaatgcggt cacatctaca
     6841 aggggaaccg taacggttcc tcgttccggt gtcggaaatg tggttttgaa ctccatgcgg
     6901 accttaacgc gtctcgaaac attgctcagg cgggtatatc ttgcctgagt aggctacctt
     6961 gtcaaccagc ccaatgtagt ccagtttaac cggaactaca agcccctccc ttcagggagg
     7021 ggtagttgac caatattctc tgcgagatac catcgaatca acttcgccag gtggatgagg
     7081 catgatcccg gagagggtgc ggatatggaa ggagggnnta gagaccagag ggcggaggag
     7141 aactgggccc tggccttcgc ccaggagctg gcattggaga ggagggcgcc cctggccgtc
     7201 gtcttcgccc tgaaagagga gttcctcggc gctgccatgc gacagtacgg gttcatgctc
     7261 cgggggctcg aagaggtgga gaggaccctc agagataagg agataccctt cgtcctcctt
     7321 cagggagacc cgggagaggc cgtccccgac ttcgtaagag agcactctgc gagcgccctg
     7381 gtcaccgact tctcccccct ggcgactaag agggcctgga tggagaaggc ggaggtgggg
     7441 cttgacctcc ccctccgcga ggtggacgcc cacaacgtcg tcccctgctg ggtggcgtcg
     7501 ccgaagcagg agtatgcggc ccggaccttc cggccgaagg tgaggcgact tttgccgatc
     7561 ttctgtgagg agcctccggt tgtccgggtc cacccccacc cctgggacct cgataccggg
     7621 gtgagctggg accggctccg gggggacctc caggtcgatc gctccgtccc cgaggtggag
     7681 tggataaagc ccggagccgc tgccgctcga agggcgctac gtcggtttct cggcgagaag
     7741 ctctctcggt acgatggaga tagaaacgat ccgaccctcg acggccaatc gaacctctcg
     7801 ccctaccttc acttcggcca gatctcggct cagagggtcg ccctggaggc ggccaaatgc
     7861 gaggcagatc ccagatccaa ggccgccttc ctggaagagc tggtggtgag gagggagctc
     7921 tccgacaact tctgcttcta caaccgcggg tacgacacct tcgatgcctt ccccgcctgg
     7981 gcgaggaaga ccctggacga acatcggaac gacccgcgag agtacctcta cgatcgggag
     8041 gagctcgaag gggcgaagac ccacgacccc ctctggaacg ccg
//
LOCUS       DASM01000069           11464 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_122431, whole genome shotgun sequence.
ACCESSION   DASM01000069 DASM01000000
VERSION     DASM01000069.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11464)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11464)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11464
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_122431"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2008 a         3718 c         3548 g         2190 t
ORIGIN      
        1 gcggagatca acctcttcga ccgggacatc gccaacatct acgtcgcaag aaagctcggc
       61 gcccccatta tcctggtggg ggacatcgag aggggcgggg tcttcgccag cctctacggg
      121 acggcggcgc ttctgccccc cgagatccgg cccctggtca aagggctggt catcaacaag
      181 ttccggggcg accccgccat cctggagccg gggcttggga tgctggagga gctgacgggc
      241 gtccccgtcc tgggggtgat gccctacctc gacctggacc tcccctccga ggactccgtc
      301 tccctggggg acaagcgggg gggcggcggg ggcgaaaagg tggagatcgc cgtggtcagg
      361 ctccctagga tctcgaactt caccgacttt gagcccctgg agcgggaggc gagggtccgg
      421 tacatcgccc ccggcgagcc cctgggtagc cccgacgccg tcatcatccc cggtacgaag
      481 aacaccgtcg cagacctgga ggagatgagg gcgaagggta ccgaccggga gatcctcgcc
      541 ctccgcaggg ccggggtccc gatcctcggg atctgcggcg gctaccagat cctggggaag
      601 aggatcgtcg acctcgggat cgagaacgcc accgtcgaga tgcccggcct cggcctcctg
      661 gacctggtga cccggttcga ccggtacgag aagaggaccg tccaggtgga gaagccggtc
      721 accggaggcg gccccatcct ggggcggatt gtgggggaga gggtgggggg ctacgagatc
      781 cacatgggag tgaccgcctc cagggacccc ccggccttcg gggacgacgg cgccgtggcc
      841 gacgacggcc tggtcatggg aacctacctc cacgggatct tcaacaacca gaacttcagg
      901 gacgccttct tggactacct ctattcgagg aagaacctgg ccaggggatc cgccctccgg
      961 ggcgacggct acgacgagct cgcctccgcc gcggagaagt tcctggatct ggagcggata
     1021 tggggtatga tcggcctcga gccggaggcg gagtagatgc ggagaagatc agtagagaat
     1081 aacggggatc ctttcagcct cgactctgga gctcccgacc aggctcaagg gttggagatg
     1141 gatagaggcc cctttcggcc ttggccttct gttcaggctt aacgccccct cgttctcatc
     1201 cttcgcttgt aggcctcgtg gctcgcgaag aggaggaccg ggaaggggag gaggatccag
     1261 aggtatccat aggggacgtg agctgtgttg aagacgagct ggacgacccc gacgttcagg
     1321 agggcgtata tccagagaag ctcgaggagg atggcgacca aaagaagcct gtttttgaag
     1381 agcccccggc tgaaggccga aaactcccag gacctgaggg tccagacgtt gaagagctgg
     1441 cagctcacgg cacccaaaag ggtcatcgtc atcgcctgct tgtgaagaga ggtgttcgat
     1501 atggagaggt ctccgtaatg ccagccgttc aaaaagagaa aaccgaggaa ggcggtcatg
     1561 gcggcagccc cctcgatcgc ccccaaaaag aagtatcctc gcttgaatac ctcccggtcg
     1621 aggatgctct cgtcctttcc gacgggaggg cggtccataa cgtcttcctc cggggcctcg
     1681 ctccccaggg cgaggcctgg gagcatgtcg gagcctagat cgatggatag tatctggatc
     1741 accgataacg gcagtggaat tttcaggaag aactgtagta tatacggtac gatctccggg
     1801 atgttcgagg agagtatgta ggtgacgaac tttttgatgt tgaagtagac ggtcctcccc
     1861 tcctcgatgg cggcgactat cgaggagaag ttgtcgtcga ggaggatcat gtccgctgcc
     1921 tccttcgcca cctccgtccc cttcgtcccc atcgatatcc cgatgtcggc cttccggagg
     1981 gccggggcgt cgtttacgcc gtcgccggtc atagccacca cctcaccgtt atcctggagg
     2041 agggcggcga tccggagctt ctgctccgac ctcatcctgg cgaagaggac gttcttctcc
     2101 gccaggagct ttgccaggtc ctcgtcgggg atgtgccgga gctcgtcgcc ggtgagggtg
     2161 aggtcaactt tgagccccac cttctcggcg atcgctcttg cggtcagggg gttatcgccg
     2221 gtgaggatca tcacccttat cgaggcacgc tggcattttt ttatcgcgtc gtatacctcc
     2281 tctcggggaa ggtccatgat ccccaatagg cccagaaata caaggtcgct ttcgtcctcg
     2341 ccctctccgt aggcgactcc gaggagccga taggccttcc gctccaagtc ttcggcctcg
     2401 gcctctatcc gcgccctatc ctcctcggcc atgggccgga cccggccgtc ttcgtcgacg
     2461 agccgatctg agagtctcat taaaacctcc ggcgcgccct tggagaagat ggtctttgaa
     2521 ccgtccctct cgtagatcat ggacatcatc ttccggtcgc tggtgaaggg gatctcttcg
     2581 acctttacgt acccctctgg ccgaacccgg gccttcctgg cggcggcgac gatggccagc
     2641 tccgtcggat cgccaaaggc accgccatcg ctgatcgtcg ctctggagtt cagaactccc
     2701 gcggcgagga ggaacttcag tcgttccagg gagcccgacc tctcttcagg gaagctgaac
     2761 tcgccgtcct cgaagtaccc atcgccgctg accgatatct cctcgccgcc cgcgaggcgg
     2821 atctccttta aggtcatctc gtttcgggtg atcgtcccgg tcttgtcggt gcagatcacc
     2881 gtggcgctcc ccagggtctg ggccgagtcg aggttcttga tgagggcgtt tctcttcgcc
     2941 atgttccggc tggccaggga tagggaaagg gtgatggtgg ggagcatccc ctccgggacg
     3001 ttggccacga tgagggagag ggcgaagatg gcggcgacga gccagccctg ccccgagacg
     3061 acgccgagga cgaagaagac gccgccgact aaaagagccg ccagggtcag gatcctggtg
     3121 atcctgatga tctcaagctg catcggcgtc gccctcttct cgacctcctt agatagtgag
     3181 gcgatcttac caaactccgt ctcccctccc gtggcgaaga cgacggcgag gccgtttccc
     3241 gaggtgacgg tcgtcccggc gaagaggacg tttctcgcgt ccaggatctc ctcgctggcg
     3301 tcggcctctg ctgccctttt ggccggctct gcctcgccgg taagggccgc catgttgacg
     3361 cggaggaggt tagcctccag gaggacggcg tcggctccga tcctgtcccc ctcctcgacg
     3421 accaggatgt cgccggggac gagatacttc gagtccactt cctcgactct ccccgcccgc
     3481 ctcaccttca ccatcgatgg gatgagcttt cgaagggctt cgacggtctt gtcggccttg
     3541 tactcctgcc agaaggcgaa ggtggcgttg atgaagacgg cgccgaggat ggcatatgag
     3601 aggatgtcgt acccctcgcc aggagaatag cggttcgcca cgaaggagag gatggccgcc
     3661 acttccagga ggagggcgaa gaacgcgagg tactgcttca agtactcctt gaaatagttc
     3721 ttttgcctcc tcctctcggt ctcgttcggc ccaaactcct ccagccttct ttcagcatcg
     3781 ccgatctcta gacccgaaag gctggtgttg aggcgggcca ggaccacgtc gcctgggaag
     3841 gagcggaggt tcttgcggcc tatcataaaa aaccttttca aatttgtcgt ctggagctta
     3901 aaaatgtctt cctgttattt ttcagttggg cgggggtgat gagaggggcc tggggccccc
     3961 ggggtgtcca gaatcgaggc tcgaccttcc tcaggccccg gcctcgaggg agtgctcgat
     4021 caaggcccag aggaggtcgg agtccttaag catccccgcc agcttctgat gggccgtcac
     4081 caccggcagc tggtcgatcc cctttctcat catcaccctt gcgcactccg agacctcgct
     4141 cgtcttgatg gcggtgacgg cggggaccat cgcctgcttg acggggatct tccggaggga
     4201 tatcctcgat accccgtagt agaagttaag ggtgtcccgg gtgctctccc aggtccactc
     4261 gtcctcgtcg ttcgccgagg acatatccga catctcgacg gagtcctcga tgacggaggc
     4321 gtttatcaga tcccggtcgg agacgatccc caccagcttc agctccgagt cgatcaccgg
     4381 cgaggcctgg acgttcgcgt actccatcac ggctccggcc acagggaggg gcatctcgtc
     4441 ccagagggcg acggtcatct tctcaaagta ccgctctata ggattcttta tctccaggtc
     4501 tgccaccacc cggatgacgt cggcgacgct gacgatcccc accagggctc catcctccac
     4561 cacgggaagt cgtcttatgt cgttctccgc cagaagcctc gccgcttcga cgacgacgtc
     4621 gtcggggtgg acgaccaggg gatcccgggt catcaggagg gcggtctggt cctcttcggg
     4681 gtttcggaga aggtcggtcc tggtcaccat ccccaccagc tccccgccct tcaccaccgg
     4741 cacccccgag acgtgcctct cctggagggc cttcaatacc atgtccctgg agccggggac
     4801 cgtcacgtag gctacgtctc tgaccatcac ctcttttacc agaacatcaa tcacaaacct
     4861 tctcccccgg aaccgtgatg acgggaactg tggaggtgcg gaccaccttc tcggccacgc
     4921 ttccgaggag gaacttctcc aggccggatc gcccgcagct cccgatgact atggcgttca
     4981 ccctgacctc ctccgccacc cggaccagct cctcgccggg gttcccctcc accaccatct
     5041 tggtggaggc gaccccagac ctctcggcca tctcaccgac gaaggcggtg gcgttctccc
     5101 cctcctttat caggagatcc cttatgctcg caagagccat gtcctcgggg aggtggctca
     5161 gccttcccgt atcggcgacg tagactatgg tgacggagcc cccagagagc ctggctagct
     5221 ctatccccac cgctgccgcc cgttcgctat gcttcgaccc gtcggttgct atcattatcc
     5281 tctcaaacat ggaaaaaccc ctagatgacg gccagaacgt cggaggcttc cgccctcctc
     5341 accaggcttg ccagtgggtc cctcgacccc gccatattct ccgaccgtct gtcgatgacc
     5401 attatcctgc actcctttcc gactcggatg gcgccggcct catcatctgc gcccaggatc
     5461 cgtgcaccgt ttagcgtgca catcataaat acctgtctat cgttatgcag cagggccttg
     5521 gatgccagct ccatctcgga gaagaggttt ggcgagttga gcatgacgtt atcggtcccc
     5581 aggcccacgg tgatccccat ctccagcatc ccggcgacgt ccggaaggcc gacgccggtg
     5641 acgaggttcg accgggggca gacgacgacc ggcacccccg cctccgccac catcctcagg
     5701 tcccggggct cggccttggt gaggtggacg aggaggtcgg gctcgaggct cagcgcatct
     5761 tcgatgtcgt ctcggaccgc ctccccggcg tggagggcga ccttctttcc ctcccgcctc
     5821 gccgcctcag ccacctcctc gacccaggac cggggatgat ctttggtgct gctgatcccg
     5881 accccccagc acttctcgtg aacgctcacc gatccgggat cgggccgccc gaagatcctc
     5941 ccggccagcc ccgtcccctc catcgcctcc agaagcatct cgaccccgtc gggccctccc
     6001 tcccgaaagt cggcgaaggc cgcagttcca gaccgggcca tgtagaggag ggaacgcctc
     6061 atcccctcca ccacctcccg gcggggggcg gaggcgaggg cccgatgctt caggcccccc
     6121 gggcccacca ggtccctgag ggggaggtag ggggggtctt taaagaagga gtcgccgagg
     6181 tgcacgtggc cgttgacgag ggccggggcg atgatcccca gcaggtccga ctccacctcc
     6241 tcgggcccga tctcagcgac gacccctccc cggaggcaga ggtgcccctc gaagggctcg
     6301 agctcctcgc ccaggaggat cgtccccgaa aggactatct cgtcatccga cctcatcatg
     6361 aaagagagat cggggaggga aaatatttag ctttcctctc cgtcgtcaag acccttatgg
     6421 agaggccctt cgtattcatc aacagcgcca tgagcgccga cggaaagatc agctccttcc
     6481 ttcgccagca ggtgaggata tcggggccgg aggatatgga gcgggtcggc ctcctccggg
     6541 ccgagagcga cgcggtgatg gtgggggtgg ggacggtcct cgccgacgat ccggggctga
     6601 gggtgaagtc tccggaccac agaggcatga ggtcgaagct ggggaaaccc gaggatcccc
     6661 tccgggtcgt cgtcgacagc cggggtaaaa ccccgccttc ggcgaaggtc ctgggggagg
     6721 ggtgcatcct agccgtatcg agggccgccc ccgaggagcg gctccgcctt ctgggggata
     6781 ggtgcgaggt cgtagctttc ggggaggaga gggtcgatct tccggagctc ctctctctcc
     6841 tctataaaag aggggtcaga aggctgttgg tggagggggg ggccaccctc aactggtccc
     6901 tcgtcgaggg ggggctcgtc gacgagatct tgacttacat cggccccatc atcatcggag
     6961 gggcgagggc ccccaccctc gtcgacggcc ccggcttctc cggcggctat ccggagctcg
     7021 agctatcggc cgccgaggct ctggacggag gcgtcctcct ccggtggcgc cttctccggt
     7081 gagccttcag gacctcatcc agtcgtccca gaccttcccc cgggcggcca ggatggggac
     7141 gaccttctcg gccatatcga aggggatccc caccaccacg aagtcgtcct ccaatccagc
     7201 gttcttggcg gcgccgtcgc agccgaggga gacgttcacg tcccccttca ggatcggcga
     7261 ggaggtggcg tcgacgcaga aggactggat cccggaggtg tcgaagttca ccctgccccc
     7321 ctcagcgaag agggctgcct ggacgatcct cctcgcctgg aagggctttc cgacgaagat
     7381 gacgatgtcg ggcttcaccg acatcttctc caggggcgcc accagggtcg cgacggtgct
     7441 ccccgccttc tgggccggga aggaggagac gatcttcgag cccgcctcct tcgtttcgac
     7501 cttgttcagc ttcgagaagt agaggtttcc tgaggagatg ttctcgggaa actccaccag
     7561 gccgagcccc gccgccccgc ccttgcagga gtggagatcg gagttggcga gggtcgtctc
     7621 gccccggagc ctcgccgcct ggaccatctc gcagtagcgg aggggccctg ggatcatctt
     7681 catcttctgg gggatctcct cctcgcttct gatcaatttc acgcccacag ggtttgtttc
     7741 caggtggagt gtcgcaacca gcgtctttgc catctcatca agttccatag agaatacctc
     7801 tcagtttatg cctggagacg ggttttgcgc ctggcctaca tcactcttgc gcttcgaggg
     7861 tctgatcgaa ttgggtattt cctcctcgag gcagagtaag gataacagtc ctaaaaagga
     7921 tattaatgcc cacacctcct ctgggggccg gatatccagc cggaccagga gctatctcgc
     7981 cccttccagc gcctccatgt agcaccctct cgccctatcg ctgaaacaga ctatgataac
     8041 cctctccacc gcctcgttct cccccaagaa cctcttcacc tcggagacgg cgatctttgc
     8101 ggcccgctcc agggggaacc catacgctcc ggtgctgatg gatggaaagg cgatcgtctt
     8161 gatcccggtt tcctcggcga gcaagagaga actccggtag gccttcgcga gttgctcgtc
     8221 ctcgcaggag gcgccgcccc gccagatcgg ccccacggtg tggatcacaa acttcgcggg
     8281 aaggtcgtac ccctttgtca tcctcgcctc gccggtccgg cagccgccga gggttcggca
     8341 ctcttcgaga aggccggggc ccgccgcccg gtggatggcg ccgtcgaccc ccccgccccc
     8401 caaaagggag gggtttgcgg cgttgacgat ggcgtcgacc tccaatttcg tgatgtcgcc
     8461 gagggcgacc tcgatccgac cattcatctc cgaccacctc tctcagatcg gatggaaggt
     8521 catcttatat ccggattttg agggaaagga aggagttttc ttccccccgt tcatccccac
     8581 gatccagccc ctcgggagag gtactcctcc ctggtgacgt cgaagaggag gtcgtcgagg
     8641 cagcggtccc ccaggaggga ggaggcccgg cgggtaccga ttctccgaaa acccgccgcc
     8701 tccagggccc tgatcgagga gatgttctcg acggtggcgg aggcgtatat cgaattcaag
     8761 cccagcctgg agaagccgtg ctctacgata gcctcgacga tggcgttgcc gaggccgacg
     8821 cccctatgat cggggtcgcc gatcaggtac gagatctcgc cggtgcaggg ggtcctctcg
     8881 tagagggagg cgaagcctat aggcagatcg ccttcaaaaa tgacgaagtc ctcgtccttt
     8941 gagcacagga tgctcgccac gagctggtct atggtgaggg acatagacgg ctccatgtac
     9001 ttcgtcagct cctggtcctc aaaccagcgg aagaggaggg gggcgtcctc cgccctcacc
     9061 cgccggaagt cgagcccgcc cgccgagaat acgacttctc cctccagtgc cgccattcca
     9121 ccattggacc tagcatcggt ccagaggcgg ccccgcccga accggtccag ggcaatcctc
     9181 gctatcctca atatcagctc cgggtactcc attccggcgg cggccgccgc ccgggcgaag
     9241 ccgctccccg ccgggtttat gcaggggttg gggttcacct cgatgacgaa gggctcgcca
     9301 tcctcctccc tgatccggaa gtcgacccga gcgtacccct cgacccccag ggcggagcag
     9361 gccctctttg cgagatcttc gatccttccc ctcagctccg gctccatggg ggcggggcag
     9421 aggggaattg tattctggta gagggggctc tcctcgatcc atttcgcctc aaagccgagg
     9481 atcttcggag ttccatcggg gaggtcgaag acgatctccg agatggggag gacctcgaag
     9541 cctccggggc cgatgaagag ggcggcgttc acctctcgcc cctcgatgta ctcctccgcc
     9601 agggcggcct ggcagtaagt ctctatgact tgcgagacct tggccctcag ctccacctcg
     9661 tcttttgcga tggagtcgaa ggttatgccg atggacgcat cctccagggc gggcttgatt
     9721 atcaccggaa agtcgaggct gaacggctcc tcgccgttcc gaaatatcga gaaccgcggg
     9781 gtgggtatcc cctccctctc caggaccagc ttggagaggc ccttgtccct cgccacctcc
     9841 agggcttcgg agggggagcc ggtgaagggg atgccgagga gctcgatgaa ccccgccacg
     9901 ttcgcctcgg cccggaggtc gtcgccgaaa ccctcggcca ggttgaatac gagatcgaag
     9961 ttcttcaggg agaagagagc ctccaggctg caccggaggg gtacggcctc ggtgcccgcg
    10021 gcttccaggc tccgtttcgc ctcctccact gtctccagga cctccacctc cgccgccatc
    10081 tcggtctcgc tccggcagga gccgtctcct gggacgttgt agaggatcgc gaccttcata
    10141 tcgagatccc ctggcggctc agggcggagg agaagatctt ctctatcagc ccctcgtaag
    10201 agtagccggc agcataggcg gccttgggga agcgggagtt ctcggcgggg tcggggatga
    10261 ggccggggag ggcgttgacg tcgaagacag aaggctcacc gtcggcgtcg aggcggaggt
    10321 cgagcctgca gaggtccctc acctccaggg cctcgaagct ctccgtcgcc gccctctcga
    10381 tcctggatcg gaggtcctcg tccaagtcgg cggggcagac ggtggtgtcg aacccggcct
    10441 ccagggagtc gtagatccac ttcacctcgt agcagtcgaa cctcggcgct cccgcgggga
    10501 ggccctcaaa gccgacctcg acgacgggaa ggacctcggg cgggtttccg aggagcccca
    10561 ccgtgaactc ccttccggtc aggtactcct cgacgaggac gggctggcgg tacttcgcca
    10621 ccatctcgcc aaccaccgcc tccagctcct ccacctccct caccagggac ctggcggtga
    10681 tcccccggct cgacccctca ccgttcggct tcaagaagag gggaaaatcg aggtctggcc
    10741 tcagctcatc gcccctcctc aaaagctgga accgcggggt tcggatcccg ttggccagga
    10801 ggacctcctt cgtcctcctc ttgtcgaggg tgatggcgag ggtggtgacc cccgagccgg
    10861 agtaggggat ccccagcatc tccagcatcg ctgggatctg ggcctccctc gcctcccccc
    10921 ggatcccctc ggcgatgttg aaggcgaagt cgaaatcgcc gcggcggagc ctctcgtacg
    10981 cctcctcgtc cgcctcgacg acctcgacgg agtcgcagta gttcagcagc gccgacctga
    11041 tcgcctccac cgtcgagggg gcgtcgaact cggcgaaggt gtcgtccccc gactcccggc
    11101 tgtagtcaga ggggagaatg ttgcaggtga gggcgacttt catctcagat cctcgggttg
    11161 gggtactcgt atacctcgcc cttgtagttg gatatgaccg ccttctcctc atcggtggcc
    11221 aggaggtagt ccggcggcag tatcgggatc ttgccgccgc ccggcgagtc gatgatcagg
    11281 tgggggacgc agaggccgga ggtgtggccc tggaggtgac agatgacgtc gaagcccgcc
    11341 tggagggtgg tcctgaagtg ctcgaccccc ttcaccaggt cgcagaggta gatgtagtag
    11401 ggcctcaccc ggttcttgac gagcttctgc atcagctcct tcatcacctc ggggtcgtcg
    11461 ttga
//
LOCUS       DASM01000070            3247 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_11000, whole genome shotgun sequence.
ACCESSION   DASM01000070 DASM01000000
VERSION     DASM01000070.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_11000"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          659 a          913 c         1132 g          543 t
ORIGIN      
        1 gttcgaaagt ggttctagta gatcctggga acacaagtca gctatgttcc aggtgcggtc
       61 ttaaagtgcc gaagtcgcta tcggatcggg tccatgaatg tcctcaatgc gggctggtca
      121 tggaccgtga cgagaatgcg gcgataaaca ttttgagact ggggctacag tctctgccaa
      181 aagctagaag ccccgccctt cagggcgggg agtagtcaca cagaagagct atagatctat
      241 ggtggaatat gaagcttcca tgtgaatccg ccgtatggga tacccttccg aacataaggg
      301 ccgccttcgc caaagagctg gtggaccggg ggctgtcgca gagagaggtc tcgcggctat
      361 tgaagatcaa cccccccgcc gtctcccagg aagtcaccaa gaagaggggg tacagcatag
      421 tcttcagcga agaggtgaac cgagcaatcc gaaggctggc ggatgagatc atcctgaatg
      481 aggaggttga cccctccgat aagatctgcg agatctgcag gatgatcagg gagggggaag
      541 aggctggggc ccgggatgga tgtgaaggct gcaggtgaga tgacccggca tgatctgggt
      601 gaggggatcg ataagctgga taggctgaag gatatcctcc gggcggatga taggattttg
      661 atagccttct ccggcggcct tgacagctcc ttcctcgccg ccgtggccgc cggggtcctc
      721 ggcgaggggg tcctcctagt aaccctcgac tcggcggcga tcccccggag cgagctgcgg
      781 gaggcggtgg agatcgcccg atccctgggc ctccgccatc gggtcgtccc cttcgagatc
      841 ctggaggctg agggggtggc cgataacccc ccccaccgat gcgccctctg caagagggcc
      901 gcctccaggc tcctcaagag gatcgccgcc gaggcgggga taaatagggt cgccgacggg
      961 gtgaacctct ccgactacgg tgagtacagg ccggggatcg gggcctccga cgaggagggg
     1021 ctgatccacc ccctcgccga ggcggggatg acgaagtcgg atatcaggct tttggcgaag
     1081 gagatgggcc tccccttctc ggataagcca gcgacggcgt gcctcgccac ccgggtcccc
     1141 tacggcgagg cgatcacccc cgagaagctc cggatggcgg aggaggccga agatctcatc
     1201 cgggggctgg ggatctccgg ggcgagggtg agggtccacg gccccatggc gaggatcgag
     1261 gtcccttccg ccgacctgga gagggccctc tcccggagga tagagatcgc cggagggctg
     1321 aagaagatcg gcttctccta cgtcgccctg gacctggagg ggtacaggcc ggggaggatg
     1381 gacgaggcgc tgggtcgcgc ccccagcctc agatccctag ggtcaacctg atggtgtttc
     1441 tcaccgccgg ggcgagcccc agggtcagga gggcgaagag ggtcaggttc ttcagggacg
     1501 gatcatcccg gagggctccc tcgacgactg cgaggacggg gaggaggatg aggagcttga
     1561 gggggaacat caccagggcg gtcccggcgg cctcgatcaa gaggctgggg acgacgtgct
     1621 tctcgacgta gccgaaccag tccaccccga cgtaggtgga ggcggcgtcg accatgtggg
     1681 cgaagaggat gaggaggctt tgccctcccg atatggggga ggagatcagc ctcgacccca
     1741 ccgccaccaa ggccgtcgcc gccgacccga gggctaggat cgcgatgggg atcgtgctcc
     1801 tctccacccc caggggggcg aggagggcga ggtttcctgc ggtccagagg aggccgacgg
     1861 cggcgtaccc cgaaagccat cgatccccca gggcctgccg gcagaggagg agggcagtca
     1921 tcgtccccgc ggcggcgagg aagtatatca gaggggttat cagcaggtac tttagggggg
     1981 ctgccaccag ctccgcatcc tcgaccaccc ggaggctcgc ccccgccagg acgtaggggg
     2041 cggtggcgag gaagaaccgc tcgtcgaagg agacctccag ccgccggagg agcctgagga
     2101 tgagaaggat catccccccc aggattaggg cccaagtgac ggtgttgacg gggttgtatc
     2161 cggcgtcgta gaggatgggc tcgacgtagt ggtggtatat ccaggggtgc aagggtccca
     2221 gctcctgccg aaggcctccg accgcaggct gagggagaat ggtggcggca tggtatatta
     2281 gcggatcgtt cccgggtagg acgacaggag ggcgaagccc gtctgggcag agccggtgag
     2341 ggggcaagag gtcagaccga ccacccctgc cacagattcc ctccatcggg ggcgggaagg
     2401 gcgcggaaga tttatatagt gatggtggcg aagagaactc gtccgttttc tgattttaat
     2461 tcgaggtatt tagatggtcg aggtgttcaa aggcacaact acggtgggaa tcgtctgcaa
     2521 agatggggta gtcctagctt cggagagccg ggccaccatg ggccacttca tcgccagcac
     2581 cacagctcag aagatatacc agatcgccga ccgcgtcggc ctcaccaccg caggagtggt
     2641 cggcgacgcc cagtccctcg tcaggatgat ccaggtggag tcgaagctct acaacatgca
     2701 gaggggcgag ccgatgacgg tccgggggac gacatccctt ttatccaacg tcctcgcctc
     2761 gaggcggtac ttccccttca tggtccagct cctcctgggc ggggtggaca agaaggggcc
     2821 ccagctcttc tccctggacg ccctcggagg ctcgatccag gagcagaagg tggtggccac
     2881 gggatccggc tcccccaccg cctacggcgt cctggaggcg ctctacgacc ccgagatgaa
     2941 cgtcgaggag ggtatgaagc tggggatccg cgccatccac aacgccatga agagggattc
     3001 cgcctccggc gacgtcattc aggcggtcaa gatcaccgcc ggggggtacg aggtggcaga
     3061 tgagtccgag gtggagaaga tccggcggtc gctctgaaaa ataaactttt tttggatggg
     3121 tttaattgtc tgttgaggaa gtgctccttg agctgaagcg gcgggtgcag accaaactgc
     3181 ctccgaacat aaacgtcacc ggcatagaat tcgagggccc cgagctggta atatacaccg
     3241 acgaccc
//
LOCUS       DASM01000071           10275 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_69844, whole genome shotgun sequence.
ACCESSION   DASM01000071 DASM01000000
VERSION     DASM01000071.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10275)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10275)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10275
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_69844"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    2206..2227
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1674 a         3457 c         3184 g         1938 t
ORIGIN      
        1 tggaggacct cgacgaagat gtccaccacg gaccggggga agtaggaggt gaggatcacc
       61 ggtgccaggg cctcccggct gaccttcgat acctcgtagc tccgccggtc ggggccgggc
      121 ctcttcctct cctcgacgga gaaggaggcc atgttgtacc tgtacctgac tatcgcccgg
      181 gtgacctcct ggagatggcg ggggtggacc tcccgggggc cgtagacggc cgctatcact
      241 ttgttgtccc ccatctcgat gtagcaagag ccgtcggccc tctccaggac gcccacctct
      301 atcttgatgg ggcggagctc gtcgggccgc ctcccgtcga gcctcaatcc atctttgaag
      361 aatgataact tctcatccat gccgatcaac ttctcgcctc tttctcagat ctcagaaact
      421 ccattatcct gtcggtgagc cccgtagtat gagcctcctt ctcgatgatg aggatcgtct
      481 tcagggcaag gtccatgtcg tcgtcgttgc cgtcgaccca gatcctgccg ttctggccga
      541 cgaagaggtt gcagttcagc tccttcttga gcatatttat catggagcct cccttgccga
      601 tcagcctcgg gaccctcgtc gggcttatct ccgccaccct ccccctcgtc aggatgccga
      661 gcctcctgtc gttcatcgtc agctcgacct tcatggtgga gtcgacgtcc ttgaccctgg
      721 tcatcacgga gcttccgatc cggaggtacc tggacatctc gttcatctcg atcttcctag
      781 gcagctcgga tatgtggagg agcccctcgt agggggagtt gatgttgacg atccagttgg
      841 agaagactat gtcctggacg gtgcctatca ccgtatcgcc cggggcgggg acgtactttc
      901 cggcgagggg tatcacccgc accttatcct tgtagctgct ggccatcccg tagaggaggg
      961 agcggacctt cccgtccttt acgtaggtgc cctcccccga cgctttgggg tcgtccgata
     1021 gaaggtctcc gggcaccacc acgttgcggt cgttattgtg atccattcct aaatcctcga
     1081 tcaaatcgtc atcgtttcaa gagcttcgtc tccgcatccc ccttggagag gcggttgacg
     1141 aggccgtaaa actcgttctg aaggccggcg gggagccgaa ccaccccgat ccacgaaccg
     1201 tcgttctgcc actgctccct ctggagcttt ccgaaggagg cgatctcgcc gtaggccttg
     1261 ggggcgtacg ccggcgggat cttgaccgcc acgtccacct cctcgaacct tatcggtatt
     1321 atcggcctta tcgccttcat ggcgaggttg accatctcct ccaccgactt ggtgggatcg
     1381 acgttgaagt gggcctcttc catcgccctt tcgatccggg cgggcggatg aggggtcccc
     1441 gtctgggggt tgatggcgtt ggtggctatg acgtggacaa cctgccgccg cttgttctcg
     1501 atgatcttct tcctctgatc agccgtcagc tggatctcac ccttcttgag gatcttcgcg
     1561 gctatcgtca gaacgtccgt cgtctcgaag gcccggagga gatcctcctc ggagctcctg
     1621 tcgccgcggc ccgcgttctc gaagacgtcc tcggcggcga ggatcttctc caggtccaga
     1681 tcgtccccct gacgataggc aagagccccc tcggggtcca ccaggacctc gaagaccgtc
     1741 ccgtgggtct tgagcctggc cgtcaccgcc tcctccaggg tgaccatctt cacgcctcgc
     1801 cagcaccctc gccgccgatc ttggccttca tctccgccac ccgggaggcg acgtcatcct
     1861 cttcgagctt acagaagagc ttctcgtgga gcttgatcac cccgatctca gtggtggagg
     1921 cctcgaactt cccctcggcg gccttgtaga gcgcctccag ccccaggagg atcgcctcgt
     1981 cgcagttcat atcctcccgg tacttctcct cgaagatctc catgacggca gaccttccag
     2041 cgccgatgcc ggtggcctta tactcgagga gggcgccgga ggggtcggtc tcgaagagcc
     2101 gcacccgggc gtcgtcggcg ccgacgatga ggagcgaagt cccgaagggg cggacgcccc
     2161 cgtactgggt gtaggtctgc ttgaagtcgc agatcttctt ggccannnnn nnnnnnnnnn
     2221 nnnnnnnagg acccgggcgt cggcgacgag gccggaggtg gcggccccaa tgtgctggtc
     2281 gatctggaat atcttctcta tcgactcggg ctccatcagc ctgctcgtga tcctcttgtc
     2341 caccaggacc gccacgccgt cgaccgcctt tatccccacg gcggtggtgc ctctcctaac
     2401 ggcctcgcgg gcgtactcca cctgaaacag ccgcccatcg gggctgaaca ccgttatagc
     2461 cctatcataa cccatctgag gtgccatctg catcaatatc ctctccgcgt aaatcaaaag
     2521 aggtcagtcc cacgtatcgt gtgcctgatc cttccgtcat cttcgggtcg tctggagaga
     2581 ggtcgatctc ccgaccacga actcgcacca cggatccaga aactggacct aaatcgactc
     2641 ttctcttatt ttcgtcgggc tgtaagatcg attgcggtat atacttttct gtcgccgctt
     2701 ttatcgagcc cgagatcccc ctaaccctga tcgccgccct catcccccct acggagttga
     2761 cggtggcgag gagggcccgg cactcctccg tcctggtatg agtggaacgg aggaggccga
     2821 accggccgtc gaaggagatt acccggagcc ggttcttcgc cgcccccaga tctccgaaga
     2881 gggaggtctg ggccgccctg atctcggcga cgaggtctcc acccctcacc cgcccctccg
     2941 cctccacctc aaaggcgatg tacctcctcc ggtcccggag gacgggaagc cagctcctca
     3001 aaccagctcc acccccaccg acgtccacct cctccggttg agggcgagga tcgattccgg
     3061 aagggagaac gcctcgaacg ccctatcccg atcgaggccg aggagggcgg ccatggccgt
     3121 cagttcccgg ggggaccgga ggtcgagatg ggaccggccc cccgtggtga tcatcagggg
     3181 aatgtcgaac tttccgaaaa ggtcgaggtc ccttctcacc gtctccatcc acctgatccg
     3241 ggccgggccc cggaggagga tcatggggga gaggtccagg gccatagcca cctgattctc
     3301 ctgggctgcc ttcgccgccg ccactccgat cccggcccgg ccctggtgag ggtgggcgag
     3361 gaggtcgacg ttcgggtcct cgcaggccgc ccggttgatc ttctcatccc ccccgtggac
     3421 ggcgaggaag tcgaccctct cccggaggga ggcggccttg gagtggaggg acctctggtc
     3481 agaggcgacg atctcggccc cgatgaccac ctcgatcccg gccaccaccc tcgccgcctc
     3541 taccccgatg ggccgaccgc aggtggtggt gtggtttgtt atgatgatcc cggcgtagcc
     3601 gagcctcttc gccgccaggg cgagcctgct cgccgaggcc gagccttcgg ggagggagtg
     3661 gaggttcggc tcgaagggcc tcataagagc tctccggctg ccagaaccgc cttctcccgc
     3721 cgggcgggat aggactcaat atgggcggat acggtgatcg agtccccgga gtcggtgagc
     3781 cgggccaccc ctcgataggc ggcctgcttg tccagccgaa agtggaggcg gcattcgtcg
     3841 tcgacccgga ggtccacctc ccggcggagc cgcgccatat ccccctccgg caggacctcc
     3901 ctcagccggt cgaagaaccg cagcccctcc ttcttccgga ggtcgatggc gaggatgatg
     3961 atctcgtttc cgtggtggcc gacgacccgg gttctggtca aggactccgg gggaacgaag
     4021 agggataggg cctctctcac cttctcctcg acctcggtgg aggctacgaa ggctcgaaag
     4081 gtcaccctgt gaatcaaatc actttcctct gccttcgttt gccctgatgc tggggcggac
     4141 cttctcggcc ccgatcccct tcttccgcag gcccctgccc cgctttccgg cgctggtctt
     4201 cccccggtta gccctgcccc ggtggcttcc gccagcgatc caggcgaggt cggggtcgga
     4261 ggctatggcc ggatggttcg ggtcgacgag gatcacctcg taccacttct gcatcccgtc
     4321 ttcgcccacc cagtaggagt tcaagacctc catgttgggg tgccttcggc ccgcccgctc
     4381 ctccgcgatc ctctggaggc tcttgccagc ggtgatcttg tttgagccca tgtgcttgga
     4441 ccttcttccg cggatgtacc tggacttcct ccggccgccg cgcctcacct tcaccctgac
     4501 gacggtgatc ccctgcttcg ccttgtagcc gagggcccgg gccctatcga gcctggtggg
     4561 ccggtcgatc ttctggacgg aggcctccct ccgccaccca atcagcctct cctgccgcag
     4621 ctcgccaacg tagctctcgc tgggggtctt ccacgcgtcg cggatgtatg cataaaaaga
     4681 cttcaatagc ttcacctcga tccggttcgg ccacctggac cacattccgg accggacttc
     4741 ctatcggctt ataggagata aaccttttcc ggccgggtcc tcagaaaaaa cccctcccgc
     4801 cgcggaagcc gtcttcggcc cggcggtcgg gatcggccgg gacccctaaa ggaccatccg
     4861 ggcccgcccc cggccggagg tcctttcaat cgtctatccg ctccgataaa agccagcggc
     4921 ggggctcccg atcctcctcc agggcgaagg aggcgaggag cctccccttc accttcaccc
     4981 gggtgtagac gagcttttcg ccgttcttgg tatcgaggac ggccagaagc cggtccatct
     5041 cgccgtcgga gagctcgatc agcccctccc tcccggtgag ccagttgaag accctcacct
     5101 cgtttatctc ccggacccgc ttgagggtgg agaggtcggt cccggcgtcg atccggacgc
     5161 tcctcccctc ctgtggagac cagccgatcc tcttcttctc cgaggcgatg aaggagatgg
     5221 ggacccgctc cccctccagg ggcttggggt agaggccgga aagcctcttc tccgccccct
     5281 cctccaaggc ggcagagtcc cgctcctccg ccccgggagg cctcctgggg aggcgaacct
     5341 ccccccccgc tccgtcttcc atcgtcctct caccagccgt caaagatatc cgatccagcc
     5401 cccggaccga tggggttcgc ctcccctggg ggtcaggtcc tcgcctcgta ctcgatcctc
     5461 tgccggagga tgcagccccc accccggagc ccccccatgg ggtgcctggg ggtccccatg
     5521 gagttcatgc acctcgccat aaccgccgcg ccccgggcaa ggccgtcgtc gacgaagacg
     5581 atgttcttct cgggctggtc gtagaggccg agggtcccga tctcctccag gatcagctcc
     5641 ggcttgttcc cgcttatgcc ggcgcggccg gtgatcccta tcgccgtatc cctggtgacg
     5701 aggccggcat cgacggccac cttcaccagc cgccgggcgg tgatggcggc ggcgacgtcg
     5761 atcgccgcaa agagggtctt cagccccttc tcctggtgga tcttcctccc gagctgggcg
     5821 aggcggttga gctcgcttcc gttctcgccg acgtcgcagc ctatcaggac gaccccgatc
     5881 ttctccgcgg cggcggggtc gacggggact gagccgtacc tggacctccc gacggggacc
     5941 cgctctatcc tgatatgctc atcgatcacc ctcgcgtagt cccgggcggt cctatcgacc
     6001 ttcaccttct ttccgctgaa gaggtcgagg gtcgtccccg tctgggggtc gacgagcccc
     6061 gtcccctgga tgagggcgtc ggggatcgcc cccgccaggc cgcagaagtt gccgatggtg
     6121 ttagagtaag gcatctctgc cgaggtgatc ctcccgtcga gggtcgtccc gaagtcgagg
     6181 gatagacacg ggtttcggaa gtcgacgtcg gtccacttcg acccctcctt tatcccggcg
     6241 gtggccagct ccccctccat ctcgttggcg acgatctcga ccccggtgga ccccttgggt
     6301 gggaagaccg aagccacggg gccgttgaag acgatcttct ccatcaggct gtagggccgg
     6361 aaccgatcgg ggatgttctg gatcgacatc gccggggtca tcagcctcgg ggggaccccg
     6421 gcgatgaggc agccgtcggc gagggcctgg atgaaggtcc ccacctcgtc cggcgacgag
     6481 aagccggcga cgacccccgt cgaccggaca acgaagttca ggtcgtcctt gatggagagc
     6541 ctcgccgtgg cgtggctttc gaggagggtg tcccggacca gctccgagac cgactcccgg
     6601 gtgagggggg tgccacatag ggtgtgggcg aagacctcct cccccggcct cggctttcgc
     6661 acgtcccggg tcatcttcac cgtcttgttg acgatccggg tctggccgtt cttcatgttg
     6721 gtagcggtga ggatgcattt ggtggtgctg ttgcccacct ccacagaggc gacgatgtag
     6781 tagggctcct tcttaagctc caggatgtcc accggggggc tctcggcgat cctgggcttg
     6841 ggcttcgacc tgaggagacg ggagatgctc ataggtctcg ggacgaacta tcagaccata
     6901 aactcttcgg gccccgccgc agaatggttc ccccccgtgg gtccgaaagt ttgatccctt
     6961 taggaaaaga acccaccaac gtgtattcag cagcggtggt gggcgcgggt cccgccggga
     7021 gcaccgcggc ccgctttcta tcggagatgg gccacaaggt cctcctcatc gaccggaagg
     7081 aggtggtggg agtccccaag cagtgcgccg aggggctctt tgccgcttgc ctcccggagt
     7141 ttgagaccgc cgtcggcgac ttcatcaccg gcaaggtccc ctacctggag gtggtattcc
     7201 cgaaccgtgc gagctacagg gtgagaggag aggtgttgat gctcgatcgg ccccgcttcg
     7261 accagcatct tctgaggctg gcggagggtg caggcgctga agtcgtcctg gagaggaagg
     7321 tcgaggagat cgaccccgaa tccggcgtcc tccgcctccg ggggggcgag acgatcaggg
     7381 cggaggcgat cgtgggggcc gacggccccg cctccagagt cggaagggcg atgggagcca
     7441 gaaaccggct ccttcccgca gcccaggtgg agatgacctg gcccgaggac gacgccatct
     7501 acgccatcat cgaccgcgac ctcccttccg agccctactc ctgctggatc ttccccaaga
     7561 acggggttgg caccgccaac gtcggctgct tcgggacggt cccgaccctg gaggctttca
     7621 tatccaagta cagaatcgag ggcgagatcg tggagaagaa cggcggcgcc atacccctgg
     7681 gcgtcgccgg aaggctccag cgggggaggg ccgtcctcat cggggacgcc ggggggctca
     7741 ccaacccctt caccggcggc ggcctctacc cggcggcccg gtccgcccga atcgccgccg
     7801 aggccatcga ccgcttcctc cggggcgaga cgaagaccat cgagtacgag ccgcgaatga
     7861 ggagggactt cgtcgcctcc gacctccatc tgaaggcgag gaacgccctc gcggccctct
     7921 ccaacgacga gctgtccagg ctgggcacgg ccatggacgg cctggtcttc cccggcctca
     7981 agttcagcgc cagccccagg ctcgtctttc gaggccttcg ccatccgggg atcgcaaagc
     8041 cgaagtacgc gcctcttctg aagcttctgg tcaggtactg cctggcgggg aaggtatggt
     8101 gagggcgatg ggctgttgag agggattcga ctccgggctg aagaggagat cttcttgagg
     8161 caccatcttc ccggcgggat gggccagggg gggtaaatta gagttttctt cttaggggta
     8221 gatctgcccg cggatcgaaa ggcgagctgg ggtaaatctc agccctccca cctccgggat
     8281 tgagggtgac cccggaagaa tatcctttta cccaccgaag tctccacccc tgagatagga
     8341 gagatcccca tggccccaaa atacctcctg ctcctggcct tgatcctcgc ttccgagatc
     8401 ggcggagcta acctgaccgt gtcccatcag gtctccccct gccagatctg gcctgcggcc
     8461 tcgggtaggg agccctccat ggggacggtg accctgacgg tggcggggga ggggcagaac
     8521 agtacggtgc cgatagacgt cgtcctcgcc atcgacagct ccgccagcat gacggagacc
     8581 gaccccacca agatgaggct cgacgccgcg aggagcttcg tcttgaggat ggaccccgcc
     8641 cgggacaggg tcggaatcgt cagcttcgac gacgacgtcg acttctccct ccccccgacg
     8701 ggggagtatg cccgggtcct ggaggcgatc gggggggtgg acagcgacgg aagaacggac
     8761 ctggaccggg ggctagcgga ggcgatagac ctgttggccg ccaactcgac cgccggtccc
     8821 gatgagcggg cccggtttct ggtcctcctc tccgacggcg acggggacta caccccctcc
     8881 gggaggagcg gctcccaggc ggaccgggcg aagggtgagg gggtcgtcat ctacaccatc
     8941 ggcctcgccc tcggcgaccc cgcgaagagg agcctcgagg agatggccga ggcgaccgga
     9001 gggaggcact ttgacgcctg caacgcctcc gccctggagg cgatctacgt ggcgatcggg
     9061 gaggaggtga agaacctcgc ggggaggggg gtgaccgtca gctacaccct ccccggggag
     9121 ctctcggcga ccggcttcac cgtccccccc gccgccgact cctcctccga cgcccccggc
     9181 tctgagggga gggacctggt ctgggaggtc ggcgacctct cggccgggga ggcgtggtcg
     9241 acctcctttg ccgtctcctc ggaggccccg ggggtcttcg agctcggcaa agccggctcg
     9301 atagtcgaat accagaggcg gagcgggttc gtcgagatcc tgaagatcga gggggcgctc
     9361 ctggacgtcg ccgagctccg gtccggcgcc tccaccgccc ttgaggtccc cttcaacttc
     9421 agcgccgcaa aggagatcgt gggggccgtc cacgaggtgg aagaggagaa cgagacctcc
     9481 atcctctgga ggttctccgg ctgcaactcg gggtgcggaa gggactgggc cttcctctcc
     9541 gacgacggca ggatcgtcgt cggaagcctc cgccccttca cccttgctac caaggactcc
     9601 ctggtggaag acctcatcca ggtgatggag atcatagccg cggcgggggc gaacgtctcc
     9661 gaatacaaca ggtcgacggc cgagaacgcc gccgagttct acgccccgga gcggggggtc
     9721 taccaccaga taagctactc cttcggcgcc gacttcgacc tggtcctggt cgtccccaac
     9781 tccaccctcc gggaggcccg cctctcggtc accggccagg agctggaccg gttcggaggg
     9841 gtcgccgacc aggagtacta tgtagacggc aactacgtga cgggctgcga gcaccacgcc
     9901 tttccctgga acggatactg caccgccgag gcggccgata ttacggggat cgtccccccc
     9961 ggcgaccacc tcatctccgg aaggaagatc agcgaccccc acaccatcat ccttgaggcg
    10021 atcaccgccg aggagcccga gaaggagttc ttcctctaca gcagagacta tcggtcggtc
    10081 tggatcccgg cgaagaccaa cgccctccgg actcctgagg agatgctctg ggcatcttca
    10141 gagaggatgg gttgacaggg ccccggaccg agcccttcag ccctcccggt tgacgacctc
    10201 gaagccgaag gactccttca ggaccgcggg gttgagcctt cccgccacca gggtccgcct
    10261 cgaacgaagg tcgtt
//
LOCUS       DASM01000072           25973 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_792, whole genome shotgun sequence.
ACCESSION   DASM01000072 DASM01000000
VERSION     DASM01000072.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 25973)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 25973)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..25973
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_792"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         5138 a         7987 c         8264 g         4580 t
ORIGIN      
        1 gtttactaca taaagttaat gatttggtca gagataccga ccccgggcct cgatctcccc
       61 gaccctcctt aaaacctccc cggcccgggc ataggttcgg gcgtagcccc ggccgaaggc
      121 agagatcagc tcctcgggcc ggtcgtggca gctcctgacg gtctgaaaaa atacgtggac
      181 atccaccccc tgggcctcga tcccctggtc gtggaaggcg aggccgaagt cgatgaagta
      241 gatcttctcc ccctggagga tcatgttgga ggtggtgagg tcgccgtgga cgatcccgca
      301 ggcgtggagc cttcccacca gctcccccac cccctcggag agggccggat cgatccgatc
      361 cttcagcttc accccctcca cgtgctccat ggtcaggctg aacgaatcga catccaggac
      421 gatcggcgtc gggacgccgc acctgcgggc ctccgagatg atccgggact ccatcaccgt
      481 cctctcccgc cggatcctct cgtcgagggc ctccatcctg taccccttgg gaagcctcca
      541 cttcgtcacc ctctcccccg ccctggtgat caccgcctcg gcgcccctcc cgatctcctc
      601 agccatctcc cgccaatccc ccatctctat ctctgcatcc tctggatcgt ttaggatgag
      661 atcttggaga tcctcccctt atatcctttg gccatcccgg gggcggaagg cgggaagaga
      721 ggcccgtcgc ccccttgagg ccccgcccga gggagggaga ggccggaccg gctcgaccct
      781 cccggaggag acccctgctg gtggagagaa gagctttatc cttccaggac ggataactga
      841 tcgtgatcat agatgtcgtc gtcgtgagca cctcccgatt tcggaggttt ggggcggcag
      901 cagagccgga gggggcggag gacctctcgg ggagggcgat cctggaccgg cttgcggccg
      961 ccggcaggag cttcacctac aggcttatcc ccgatggggc ggaggaggtg aggagggcga
     1021 tcctcgagtc ggaggcggac gtcgtcgtcc tctgtggcgg gaccggcctc gnngaggtgg
     1081 ggacgagggc gatcctcacc agggcggcgg cgggggtggt cggcggcgtt cccgtcttct
     1141 gcctcccggg gtcgccatcg gcagccagcc tcgggatcga gcttatcctc gccgaagtcg
     1201 atcacatcct ccaccacatt tcagaatgac gaaaattata atacattttg aatttgagag
     1261 aaaaggatgc agtgatcctt ttataggggg aatctcttta ataagagcgg cgatctgccg
     1321 cggaggtgag taccatggaa acagagattc cggtgcggga tataatggcc cgacctgtga
     1381 tcaccatcga ttccaaggtt gatgtggtga aggctgcgaa caagatgttc tccgcaaacg
     1441 tgggcagctt gatcgtgatt cacgacgacc agccgatggg aatcataact gagcgggacc
     1501 tggtcgggaa gatagtcgcc agagccgcaa accccagaga agtgaaggtg agggacgtga
     1561 tgtcctcgcc cctgatcacc gtccctccag acgtgagcct gagcgaagcc gcgataaaga
     1621 tgctcaaatc gggggtgaag aggctcccgg tgatatccag cgacggaaag ctggtgggga
     1681 tcatcaccga caccgacctc gtctccgggt cggcctccat cgggatgggc gagatcctca
     1741 gccatctcat cgagatgcac agggacagcg tccatttcga ggagcagaag gagatgataa
     1801 gagggatctg cgagcggtgc ggccagatat cagactctct ggagtcggtg aacggcgagc
     1861 tcctctgctg gagctgcaga gacgggaata gatgagctta ttggagtcat ccggaattga
     1921 ggaggaggag gagggctaga tatgggagtt tcagttgaag agatcatgtc aaaggacccg
     1981 gtcagcgtag aggaaggaga cttcgtcacc cgggcgcgac agcttatacg cgacaaccag
     2041 cttagggggc tgccgatcct caacggcgag gggcgagtcg tcgggatagt caccgaccgg
     2101 gatatgttga aggtaccgtc taccaggtct aacgtcaccg tctccggctt ctccgtggag
     2161 gcccccctgg tcaccccaaa gacggatatg gcagaggcgg cgaagctcct cctttcggcc
     2221 aattatgcga tactcccagt cgtcccgtcc tcggaggacc gccggctcct gggggtggtc
     2281 agccccatcg acatcttcaa gatgatcgac ccggagaagc tcgccggaaa gagggcgaag
     2341 gatgccatga gcagggagac gaagacctgc tctccccagg acccggtgac gaaggtctgg
     2401 gacgccatgg tcgattccga cttcaccggg atgccggtgg tggacgagag ggaaaggccc
     2461 ctggggatga tcacccggtt cgacataata aagacggggg gcgcccggat ggggagagac
     2521 accaaggacg tgatgaaggt ggagaagctg atgagcaccc ccctcttcac cgtagctccg
     2581 gagtcgagcc tccgcgacgc ctcgaagctg atgctcgacc gcgagatagg gaggatatcc
     2641 gtggtggagg acggaaagct cgtcggaata atcgacagat acgacgtgat caaagccatc
     2701 tttggtgagg gacaatgaag agacattcag aggatcggaa ggattccaag ataagaatgc
     2761 cgggagcgat ggacagggga ccggtccagt tcgattccag agttgccaag cgtgcagggg
     2821 acgttctcac catagcaacc aatgaggtgg tgacggctcc tcccaccacc accatcatcg
     2881 gcgccataaa gatcatgacg tcttacggct tcgggaggct gccgatagcc gatgccggaa
     2941 cccgacggct cctgggcttc gtcaccggcg tggacgtcgt agacttcctc ggaggcggca
     3001 taaggcacaa cctcctaaag aagaagtacg agagaaatat cctcgccgcc atcaacgccg
     3061 acatccggga gatcatgagc cccaggctca tatccatcgg ggagaaggcc tccgtggagg
     3121 aagccctgag gctgatgtac gagaaaaacg tgggaggtct tccggtcgtc gacgatgagg
     3181 ggaggataaa ggccctggtc accgagcacg acttcgccaa gctcgtcgcc ggggtgaaca
     3241 ccgggatcaa ggtggaggag ttcatgagcc cctcggtcat caccgccaac ggccagatct
     3301 cgatcgagaa gacgacgaag atcatggtcc agccaggagg cttcagaaag ctcccggtcg
     3361 tccaggactc catcctgatc gggatggtga ccgcctccga catcatgaga tacctgggga
     3421 gcggcgaggc gttcgagaaa gtggtgaccg gcgacatcgg cgatgtgatg agccagccca
     3481 taaaggtcct ggtgcggagg gacatcgtct ccacggagaa ggatgcggat ctcggctggg
     3541 cggcggagac gatgatggat aagggcgtcg gcgcccttcc ggtcatggac aggggatccc
     3601 tcctggggat catcaccaag agggatttca tcagggcatt tgctgaggag aaacgaggga
     3661 tcattccatg agcaaggtaa aagactacat gcaatctccc gtctacgtca tcgataaaga
     3721 ggagccggtc cagagggcga ggaacctgat gctgaagcac tacatcagca ggctcctcgt
     3781 gatggacgga gacaggctgg cggggattgt gacaaaacac gacatttcac accgactgag
     3841 ccaggcagcc cccgaatggc ggaggaggcc catcagcaaa gtacccgtca ggctggtgat
     3901 gaccgaacgt cccatcacca tctaccccgg ggccaccgta gagcaggccg cggagatcat
     3961 ggccgagaac ggaatcgacg gcctgcccgt cgagagggac gagggcgaga tcgtgggcat
     4021 aataacctcg agggatctct tatcatactt tgtggggaag atccccgatt cgaaggtggg
     4081 cgacctgatg gcccagggcg tcgtcagcgt ccacaggcac cacaccatcg accacgtcct
     4141 cgatgagatg gtggagaacg gcgtcgacag ggtcgtagtc tgcgaggaca acgccacccc
     4201 cgtaggggtg ataacccaca ccaacctcgc gatctcgggg ctgtccgacg ccaaggacga
     4261 actgaagact aagagcatca agatggcgag gaaggagagc accgccggga ggaaggagta
     4321 caggtacgtc aaggaggtcc ccctggtggc ggaggacgtc atgtcctacc cgatcatctt
     4381 caccggcccc gaggtcaaag ccatagatgc cgccaagatc ctcctggaga aggagatatg
     4441 cggcctcccg gtggtggacg acgggaaggt ggtggggttc ttctcgaccc gggacataac
     4501 cgcagaagtg gccaggtggt cgtaatcatg ttggtaaaag atatcatgtc aacgccggtg
     4561 accatagaca agtcggagag gatcgcccac gctctggacg tcatggagaa gaacgacctc
     4621 cgaaggcttc tcgtcaccaa cgacggcaag ctcgccggca ccatcaccat gaggcagatc
     4681 gcgagggtcc tggggacgag gcagcgtctg gggatgcccg cctcctccct ccacgtcgcc
     4741 accgccacct cggatacgat gatcaaggcg gatcccgaga tgaaggtcga tgacgccgta
     4801 tttctcctcc agaagacctc agtcctggtg gtgatggatg gcgaggatat tctgggctgg
     4861 gttaggccga aggacatgct gaaggcgatc aggatgacgg ggcttgccgc cgacgccatg
     4921 aggactcccc tcaccgccac ccccaccgac cgactgatcc acgtccggag ggtgatgctc
     4981 gaccaggacg tcgggaggct tccagtgatg gagaacggga agctggtggg gatcatatcc
     5041 gaacgggact tggccaagtc cctgcgggcc ttcagagatc tggttcaagg cggccagcag
     5101 gagcccagag tcaaaaacct cctcgtatcg gacgtcatga gccggggggt aaagtcgatc
     5161 ttcgtcgata cgcccttgga ggaggcgaga aagacgatgc tggatctgga cctgggcggg
     5221 atgccggtct taaaccagaa gaacgagctg gtggggatgc tgaccaggcg gtgcttcttc
     5281 gactacctgg tgaagcagaa atgaacctcc gagccgcggc ggaagggctg ggcctatcgc
     5341 cagagaggat ggaacggatg atcggacggg tggtccgatc ttccgactgg cctgcgcccc
     5401 ggctcctcag gttcgacaag gccgtcgccg ggatcgaggc ggggacggtc gtcttcgacg
     5461 aggacgagat cgtcttcggc taccccaaga tcaggcgggc gatgatgctc gaccccgccc
     5521 tccggagaca tttctcctcc ggggtcctcg tcgaggagaa gatgaacggc cacaacgtcc
     5581 ggctggcgag ggtgggggaa agtaccgtcg ccctaacccg gggggggttc atctgcccct
     5641 actcgacgga ggtggcccgg gagcagatac cccccgagat cttcagggac cacccggacc
     5701 tggtcctctc cggggagatg gtgggaccga agaaccccta cgtccctaag ggggtctacc
     5761 ccgtggaggc gatggacttc tacctcttcg acacctcccg gaaggggagg agggatctcc
     5821 aggggcccct ccggacccat gccctcgccg aggagtacgg gatccgggcc gtccggctct
     5881 tcggcgagtt cggcctccag gaggcggcgg ccgagataag agagatcgtc cgggatctgg
     5941 gggcccgggg tcgggagggg gtgatcgtca aggacccgga gaacctcgca cgccccatca
     6001 agtacaccag ctccgagagc aactgccggg acctggagta cgccttccgc tactacaacg
     6061 actacggcca ggacttcttc ttctcccggg tcgtccggga ggggttccag gcggcggagt
     6121 gggccgaggg ggaggccgag ttcctggaga ggtgcctccg gctcggaagg tctatgctcg
     6181 cccccatgag ggagacggtg gaggcgaaga tggtggggga gccgatagtc caggaggtgg
     6241 agattttggt gagagacctc cagacggccc gggacttcga agagcacttt cggaggatgg
     6301 gggtcagggc gatcttcgac ccgcctcgga actctcgggg gggccacctg gtgaagataa
     6361 agaggctcgt cttgagcacc aacgacaaga cggagtcgat cgtcgagggt cagctctggt
     6421 gaccccgcga cggccaagat cttcggatcg gatggaatgg ttattagggt attagatcca
     6481 aattaaaggc tggtcaccat gagcgaaacc tgcgatattg agatggaagg acacctcatc
     6541 gactccctga ccctgaccaa ggctctggac aagatcatga gcatgggcgg cgagtttgag
     6601 atcaagaagt tcagggtggg caagaagaag cacgatacca gctacgtgat gatcgcgatc
     6661 tcgggggagg actccgacca cctcgaccgg atcctcctcg ccctccaccc cctgggggcc
     6721 cgggtgatcg aggccgagga cgtccacctc gagatcgcgc cccaggaccg ggtcgtcccc
     6781 cggggcttct actccaccac aaaccacctc acccacgtcc gccaccgcgg cgcctggctg
     6841 gaggtggagg acattcagat ggactgcctc atcgtcgtca aggacggccg ggcggtatgc
     6901 gtccccatag ggcagatcga gaagggggat gccgtcgtcg tcgggaccgc cggggtgagg
     6961 gtcgtcccac cggagcggcc ccgggagaag accttcttcg agttcatgtc caacgaggtc
     7021 tcctcggaga ggccctcggg ggagatcgtc aagcagctgg ccgaggagat cctggcgacg
     7081 aagagggcgg gggggaagat cgccgtcgtc ggcggtcccg gcataatcca caccggagcc
     7141 gcggaggccc tggcggggct gatccgggac gggtacgtcg atgtcctcct ggcgggaaac
     7201 gccctcgccg tccacgacat cgagcggcag ctcttcggga cgagcctggg gatggaccct
     7261 cacggcaacc tcacctccgc aggccatcga aaccacatct acgccatcag cgaggtgata
     7321 gccagcggct ccatccgcca agcggtggag gacggaaagt tgaaggacgg gatcatgtac
     7381 gagtgcatcc ggcgagatat ccccttcgtc ctcgcggggt cgatccggga cgacggcccc
     7441 ctccccgacg tcatcaccga caccgtcctc gcccagaagg cgatgttcga ggccctccgg
     7501 ggggtggaca tggtgatcat gatggcgacg atgctccact ccatcgccac cggaaacctc
     7561 ctcccctccc gggtgaagac gatctgcgtc gacatcaacc cctcgacggt gacgaagctg
     7621 atggaccggg ggacggccca ggcgattggc ctggtcaccg atataggcat attcctcccc
     7681 aggctggccg aagagatttc atgcctcgcc ggggaggcgg atgcctccgg ggcttgagga
     7741 agatcgcaac gcttaaattg agagcgaccg ctaatagggc cgcggggtct gtgccgccgt
     7801 ggcttagccc ggtaaagcgg ctgattcgta aggccggttt tggcccgata tatcagcagg
     7861 tcatgggttc gaatcccatc ggcggcttct ctattttatc cccctcttcg aaggcgggac
     7921 tgccccaacc tacctcctct ggaggaaaag ggggataccg ttgtcgtcct cgacctccac
     7981 cttccggcgt tctgtcaact ccacaatact ttatataccc tcagtttaaa aaggggcgtt
     8041 ccggagagac gcgattatgg agtccaccat caacatagag aacgtcgtcg cctcgacgaa
     8101 gctcgccgag gagttcgatc tgatcaagat cgagtcggag ctggagggcg ccgaatacaa
     8161 caaggagaag tttccggggc tcgtctacag ggtcaaatcg ccccgggcgg cctttctgat
     8221 cttcacctcg gggaaggtgg tatgcaccgg cgcaaagaac gtcgaggacg tgaggacggt
     8281 gatcaccaac atggcccaga ccctcaagtc catcgggttt gaggacgtca acctcgaccc
     8341 cgagatccac gtccagaaca tcgtcgcctc cgccgacctc aagaccgatt tgaacctgaa
     8401 cgccatcgcc ctcggcctcg gcctggagaa catcgagtac gagccggagc agttcccggg
     8461 gctcgtctat cggatcaaaa cgccgaaggt ggtggtcctc atattcagct ccggaaagtt
     8521 ggtggtgacc ggaggaaagt cccccgagga gtgcgaggag ggcgtcaaga tcgtccgggt
     8581 ccagctggag aacctggggc tcctctgata ttctcctccc ttatccgggc cggggccgtc
     8641 tctcatcggt ccggcaggag ggccagcacc cctgaggctg ggcctcctcc cccggacacc
     8701 cccattcagg ggactccgcc ttccttccgc tgggccagcc tctccgccac ctctccgagg
     8761 cggaggctct cctgagatcc cgtggacatc tccctcaggg tgagcctccc ctcctccacc
     8821 tccttcgggc cgacgatgac ggcgaagctc gccccgatgg cggaggcctg cttcagctgg
     8881 gcgccgagcc cccggcccat cacgtccagg acgatgggct gagggacgga ggaccggagg
     8941 gccctagccg ccctgatagc ctcggcccgg acctggggga cgaagacgac ggccacgggc
     9001 gccggcggcc tctcctccag ggtgacgatc tccatgatcc gatcaaagcc gatcccaaag
     9061 cccgtcgacc tcgtctcctg gccgccgagg agggccgcga ggctgtaggt cccgccgccg
     9121 cagatctgat tttgggcgcc gagccccgtc gcgtagatct caaagacggt tcctgtgtag
     9181 tactcgagac cccggacgat ctcgaagtcc accgtagcct cgacgccgta ggtagcgaga
     9241 aggtccacca gctctgcaaa gtgagctaaa tccaggtctg ctggcgcggc cctatcggac
     9301 cttgcgacgc gatccccggc agcggatgca atctccgaat cgcctgcttc cacctcatcc
     9361 tccaggaggt cgtctgacat cttggccagg tcttcggcca tctcggccgc ccgatccagg
     9421 gcccgtcgcc ctttgaggtc gataagctcc agaaggccga ggtggtctct tccgatctcc
     9481 tggagcttct cggccagaag ggtccgttcc ttcttgtcga tgaacctcat caacgtcggc
     9541 cgctcggagg cggggatcgt cctcatcata gcccggatca ggccgaggta gccgaggtgg
     9601 aggtctcccg agactccggc gctctttatc agctcctgcg ccagggcgat cgcctccgct
     9661 tcggcgtcgg ggcggtcgct tccgacgagc tccgccccca gctgccagaa ctcccggaac
     9721 cggccccgct gggggcgctc ataccggaag cagtttccaa agtagtggag gcggaggggc
     9781 tttgccgcct tctggagctc cccgacgtac atcctcatca ccgacgccgt cagctccggc
     9841 cgcagggcga ggtcgcggcc gcccttgtcc tggaaggcgt agatctnngg gcgaggtcgc
     9901 ggccgccctt gtctttgaag gtgtagatct cctcggtgat cgacggcccc gactttatgg
     9961 tgaatagctc cagatgctcg aaggtggggg tcgatacctc ggagtagccc caccgttcca
    10021 ggatttcctg catcctcctc ctgacggcgt tcctcctgaa ggagtccatt gggaggaagt
    10081 ccctcgtccc tctcggtctc tggatcgtca tgccacctcc gcccttgcct caaggcccct
    10141 tatagctttt gggagccgaa acggccccca tccagagggg ccaatacttt cgccccgcga
    10201 gcctcgacct tttgaaggcc ggaggagaag tacaggttat gaggccaaag cccaaagggc
    10261 gcggcctccg ttgccatgat cggatcgagg ccgggagagg ccccaaaaat cccccttcat
    10321 ttccctctcc cggaaaactc ccttattacg ccggacgtag tcagcaggta gcgcccgcgc
    10381 gaagaattgt gatataaagc gactgacctt atgggtcggc gatcatttat tttatcgatg
    10441 agatggaatg gtggcggtgg agattgacgc gggggccatc atgatcgccg cagcttgcgc
    10501 gatggccagc gcccttctta ccctgatctt caacatcatc gacttccagg tgagcatcga
    10561 gtcgaggctg aaggacctgg agcaccaggc cgagatcctc cagtatctgg aggaggcggt
    10621 cctcgcccag gaggttagga tcggggagct ggaggtggag gcgagccgcc ctgagccgcc
    10681 atccggtgac tgaatagacc ggggcggggg ggtaaagccg ccgtactgtg gatggaagaa
    10741 gatctactga cggggattgg ggccttttga gaaccatgcg gaaaaagaga ataaaaaggg
    10801 gaagcttcaa ctcgagattc tgaacgagct tcggtatcag tgcccgctcg cggcctcttc
    10861 taaagctccg gaagcctcct cgacggtcgc ttcgacctct cctgcagcct cctccgcagc
    10921 agagcccgcc tcttcggcgg cctcctcgac cgcctccgac gcctcagcgg cggccccttc
    10981 agcgccatga gcagcatctt ctaccgcacc tgcagcctca gcggcggccc cttcagcgcc
    11041 atgagcagcc tcttccgccc catcctcgcc gacgcaaccg caggcgaagg ctgcggcaaa
    11101 gacgaggaag agcgccaaaa cccagttgta tttggacatg gacagcagat cggcggaaga
    11161 ctttataagg attccgagag gggatggccg gtactcgagg atccggggct tgggggacga
    11221 ggaccggggc cttccccctt cccggcggag aagatcgaaa ccccattcgc cccgaagctc
    11281 ccctccccgc cggagagctc atatcctcga cccttcgccc cccctcccct ccgcgatccg
    11341 catcgcctcc tcggggctct cagctttccg catcccctcg acgttccagt cgccgaggtc
    11401 gatgaccagc tttcccatct tcagggcgag ggcgatctcg gagagggtgc cgtaaagccc
    11461 cgggagggcg atgacgacgt cggcgctccg gacgaggatg gcgttcctcc cgtggcccat
    11521 cccggtccct atcgccaccg cgacgaaggg gttggccgcc tccttctccc ccggcaggac
    11581 cccgacggtg acgcctccca cctcccgcgc cccctggcag gccgcctcca tcaccccgcc
    11641 caggccccca gtgatcagga cgtggccgcc ctcggcgatc tttctcccca gctccctcgc
    11701 gagggagcac ctcctctcct cgtactctcc ggctccgact accgccacct gcaaaaaaac
    11761 tcctccacgc gaggctatta ttagagcagg gtggacttat gggttgtgac cgccttctcg
    11821 aggctccggc cggagatccg ggggatgctc gcccgccagg ggatagctga gccgacggct
    11881 ccccaggaga gggcgatacc cctgatcctg gccggagagc acctcctcct catcgccccc
    11941 accgggaccg gcaagaccga ggctgccgcc ctccccctat tccacagggt cctccgggat
    12001 agggctaggc cccgggccgg gaccgccggg atggaggcgg ggaggggccg gacgaggggc
    12061 gaggagaggt ccccccccgg cgacaagatg cggtgggaga cgaggtccaa ggatcgcatg
    12121 cgggaaaggg accgccccgg aatcgaggag gatgaggccc ccggccggat ctccgccatc
    12181 tacgtcaccc ccctccgggc cctcaaccgg gacatgctct cccgcctccg gtcctggggc
    12241 gaagagctgg gggtatccgt cgccgtccgc cacggcgata cgacccaggc ggagcggagg
    12301 gcccaatcct tgcggcctcc cgacctgctc atcacgacgc cggagaccct ccaggtgatg
    12361 ctgacgggca agcggctccg ggagtccctc cggggggtca ggaccgtcgt catcgatgag
    12421 gtccacgagc tggcggcttc aaagcggggg tctcagctcg ccgtcgtcct ggagaggatg
    12481 gaggaggtgg cgggggagtt ccagagggtc ggcctctccg cgaccgtcgg gtccccggag
    12541 gtcgtcgccg agttcctcgt cggtccccac cggaggtgca gaatcgtcca ggccgacgtg
    12601 gagagggagg cgaggtttcg ggtgatatcc cctcagccgg aggcaggcga ctatgccctc
    12661 gcaaaggagc tggagtgcga cccgagcctc gccgcccaga tccgggtgat ccgggaggct
    12721 gttcggaggg agaggtgcct cgtcttcgtc aacacccgcc aggccgccga gatcctgggg
    12781 tcgagcttca ggatcctcgg cgagaggatc ggggtccacc acgggagcct ctccaaagag
    12841 gcgaggatcg aggcggaaga ggccttcaag gcggggaggc tccgggggct gatctgcacc
    12901 tcgtcgatgg agctggggat agacatagga gacgtggagc acgtcatcca gtacagctcc
    12961 cccagggagg tatcccgcct cgtccagcgg gtgggaaggt ccggccaccg ggtggggagg
    13021 acctcggcgg ggacgatcat agccacatcc cccgacgacg tcgccgaagc cgccgccata
    13081 gcgaggaggg cgaactctcg ggtgctggag gggacgaggc tccacgagcg gccgaaggat
    13141 gtcctggcaa accagctggt ggggctctcc ctcgacttcg gtgaggtcgc cctcaaaagg
    13201 tgctttgaga tcgtcaccag gtcctgccct ttccgggacc tctccttccc ggagctggag
    13261 gaggtggcgg cggagatagc tggccatcgg ctcgtcttct tcgagggcgg catcctctgg
    13321 cggaggagga agtcctggca gtactactac cagaacctct cgatgatacc cgacgagaag
    13381 aggtaccggg tctacgacgt cgtcgggagg aagcccgtgg ggaccctcga cgaggccttc
    13441 gtcgtcaact tcgccgagcc gggggcgacc ttcgtctcca ggggggagat gtgggagatc
    13501 gtggaggtcg tcgacgagga ggcggagatc agggtcgccc ccatccagag ggggggtgag
    13561 atcccctcct ggtcggggga ggagatcccc gtcccctggg aggtggccca ggaggcggga
    13621 gaggtccggg gaagggtcgc ccgccacctg gaaggtgagg ggagcggcga tggcggggga
    13681 ggtggtggcg aggctgcggc cctagccttc ctgatggaga gctaccccgt cgaggaggag
    13741 gcggcctcgg ccttcgttaa gttgataaaa aggcagcttg cggctcatct cccggtcccc
    13801 gatgagagga cgatcgtcgt cgagggggac gggacctaca tcaccataaa cgcctgcctc
    13861 ggccaccagg ccaacgaggc cctgggaagg gtcctgaccg ccctcctctc ggcgaggctc
    13921 gggacctccg tggccctgga cgtcgacccc tatcggatcg gcctggagac gccgaggcct
    13981 attctgccgg aggaggtggc agccctcctc gccggaacgg acccggacca cctcgccccg
    14041 atcctggaga tgaccctcaa gaacacctcc ctcttcacct ggaggatggt ccacgtcgcc
    14101 cggaggttcg gggccctccg ccgggacctc gaccgggagg tggtgagcct gaagagggtc
    14161 ctcggcctct tcgtcgacgg ggtcatcggc gaggaggcga tgcgggaggt cctcttcgac
    14221 aggatcgacg tcgaccgggc gagggaggtt ctggaggatc tgagggcggg gaagatcgcc
    14281 atcaccacct cctcggcgag ccccatcggc gccgagggaa ggcggggcgg gagggacgtg
    14341 acgaccccgg agcgggccga cggtgccgtc atcgacctcc tcaaagaccg gatcatgaac
    14401 gaccgggtga tcctcttctg cgtccaatgc aaaggctgga agtcgctgcg gatggtcaag
    14461 aacgtccccg aggtccccga gtgccccgtc tgcggctccc ggatggtggc tgccctcaag
    14521 ccctgggagg aggaggagat caggatcgtc aagaagccgg agggggcgaa gacccccgag
    14581 gaccggcgga ggacgaagcg ggtctaccgg aacgcaaacc tcgtcctcac ctacggcaag
    14641 gccgccgccg tcgccctggc gagccggggc ctcggccccg agacggcggc gaggttgatg
    14701 cggaacctca ggagagatga ggccgacttc taccgcgaca tcctcaaggc ggaacgggag
    14761 tacgccagga cgaagaggtt ctggtcctag tagccccaaa acttaagccc tccgaaggtc
    14821 taggtcctat ccatggcttt cgatcttttc ccggccgtcg acctccgcgg cggccgatgc
    14881 gtccagctcg tcggcggcgt cccgggaacg gaggtggtat ccctcgacga ccccctggcg
    14941 gaggcggtcc ggtgggccga gatgggctcc tcgaccctgc acatcatcga cctggacggg
    15001 gcgatccagg gggtccggat caacggcccc atcctccagg agatcgtcga gaccctcgac
    15061 ctcttcatcc aggtgggggg cgggatcagg tccccggagg atgcggcttc gctccttgac
    15121 ctgggcgtcg accgggtgat cctggggacg gcggccctcg cccgccctga gatggtgacg
    15181 gaggttgcgg aggagttcgg gaaggagagg gtgatggtcg ccctggacgt gaggcgcggg
    15241 aaggtgacca ccgaggggtg gcagcggaac ctggagaagg gggcgatcga gcttgggctt
    15301 ctattccagg agcggggggc gggctcgatc ctcttcacca acatcgacgt cgaaggtcag
    15361 gtccggggga tcgaccccga gccgacgagg aggctcgtgg aggcggtgga gatcccggtc
    15421 gtcgccgccg gaggggtcac caccgtcggg gacctcctgg tcctgagggg cgcgggcgcc
    15481 gcgggagcgg tcgtcgggac ggcgatatat acgggaaagc tcgacttcag agacgccctc
    15541 tcggccctcc gatcctgata ttgatcggtc atggagggtt tgtggaggtt tcatggagat
    15601 cgatctaggg tttatggagg aggcgtggag gtggagtctt gaggaggggt gagggaagct
    15661 ccgaggtcgg aggggcgcga gcagtcgtcg aattggacct ggatggccaa gggaactcgg
    15721 tccttgaatc cgggtcgggg ttcctcgacc acatgctgag cgccctcgcg aggatgagcg
    15781 ggatcgacct ctcggctaag gccggagggg ggatctccgc ccagaggtcg agggccttgg
    15841 ggctcgccct gggggaggcg atcgcctccg ccctggggga taagcggggg ataagaaggt
    15901 acggctgggc ggcagccccc atggatgagg cccttgcctc ggcggcccta gacctgagcg
    15961 ggagggccta cctggtgatg aggggcgagt ttgccggcga cctcgtcggg gacctgccga
    16021 ctcaggatgt ggcggcgttt ctctcggccc tgacggaggg gggccggctc accctccaca
    16081 tcaggttcga gggcgaaaac gaccaccacc aggccgagat catcttcaag gccctggggc
    16141 tcgctctgaa ggaggctatg aggagggagg gtaccctcgt cctcagcacc aaaggggtcc
    16201 tctgaaactc caggagatca ctcgaactcg atcgtccccg gcggctttgg cgtcagatcg
    16261 tagaccaccc tggagacctc ggggatctcc ccggcgatcc tgtgggatat ccgcctcaaa
    16321 acatcccagg gaagctcgat aacgtcggcg gtcatggcgt cccgggagga gacggccctc
    16381 accgccacga cgtaaccgta ggcccggatg tcgcccttga cgccggtcgc cttccccagg
    16441 accgcagcga aggcctgcca gggcaaaaac cccttcagct cctcctcgac gatggcgttg
    16501 gcgatccgga cgaccccgag cctctcccgg gtcacctcgc cgaggatcct gacggagagg
    16561 ccggggccgg ggtacggcat ccgctcgcag atctccgtcg gcatcgagag cccccgggcg
    16621 acggccctca cctcgtcctt gtagaggtcc cggaggggct cgactatccc cttgaaatcc
    16681 atatggagcg gaaggccccc gacgttgtga tgggacttga tccccccctc cgactcgatc
    16741 aggtccgggt atatcgtccc ctggatcagg tattcagcgc cgacccgcct cgcctcctcc
    16801 tcgaagaccc ggatgaaggt ctcgcccacc accttcctct tctcctcggg gtcggtcacc
    16861 ccggcgaggg cgcccaaaaa ccggtcctcg gcggtgacgg tgatcaggtt gaagtcggag
    16921 aacatctcgg cgatcttctt cgactcgttc agccgcatca ggccgctgtc gacgtagacc
    16981 gggatgagac gatccccaag ggcctcccgg gctagaaagg cgcagacgga gctgtccacg
    17041 ccccctgaga ggccgatgac cgcccttccc tttactgtgc ggcgaatctc tgctatggct
    17101 tcatcgatga actgggggac gttgatgccc ataggggcct tttggccaat tccgaggata
    17161 atagggttgc ggttatcggc ggcccggatt tcagatgaaa ccaacgtgaa ccgcacgtga
    17221 aaaataatca tttaaaggag cgggtgttag ggtatctctg cgggaagggg agtgggaaac
    17281 aacagatcgc cagacctcct atgaggatga gagggcatct ccctccgccc tctcatccat
    17341 cgtccctttt gctcatttgg tgatctattc ttcttcctgc cttttcattc acccaatgaa
    17401 gcgacgatct tcaggccccg catcgatatc cgtctctgcc tatcatttgt agacctcgct
    17461 cccgcatttc aatgagtagc tcccatcgaa gacgctggtg atggcggcat ccctctcatc
    17521 ggtgtggctc tcgatctcgt atatcgccat cccggtgaag ttcgcctcgg agctgagccg
    17581 gagcctcgtg cttctgacct cagcctggcc ggtggagttg agccggtcgg cgcgaaggta
    17641 ccgctcccgg atcatcgaga cccgcttctc gtcgatgcag aggtggcttg cgctccacgc
    17701 ggagtcgaag cggatggcgc ggcccagggg gagggcgaag gccgtcgggg atttgacggc
    17761 gtcgaaggac cgctccatct tcatcctgga ggtcctgtag ctgaggacct cctccgatct
    17821 gtaccttccc gagccatgct cagcgaagct gagggtcgtc ccgacgatcg ccttcaccgg
    17881 cgtcgcgacc cgcccacgcc tctcgaactg gtcgcaggat atcctcacct ggttcactat
    17941 cgtggagggc tctcgattgg tggtgaacct ccgccggacg gagacgaaac cctccccgga
    18001 tacctccccc tccacctccg cgacgaagtt ctgcttcacc atcttcatca ccacgacgat
    18061 ctggccgtat tctccaggaa ggagggtccc tatcgtccag acgttactgg tccccgggtc
    18121 aggcggcggg tctgccgaga tgatggacat cctcgggtcg taatgctcca cgatcaccag
    18181 gtttgttatc ggctccaata ggtcgttgcc gtaggttatg atgtaggtga tcttgccccc
    18241 ctggcttatc ctgctcggct ttccgacctt cacgttcggg ccctcgcccg gctcgaagct
    18301 ggcgatcatg ccgtttatgc tgaagtaatc gaagagccag atggcaccct cagacggaag
    18361 ctttttcgag gaggtctcaa acctgacata caatcgtatt aaatttaaat tttctgtctc
    18421 atcaatccat tcttgaatac tgaatttctg aggatttgct ctggatctcc catattcgag
    18481 gtccaggcta ttgagctctg tccattcatc gatctccaat cccatacctt cgacgccata
    18541 tctcgacgta aatagcttta agtcggattt gctgctactg gtccactttt catctccatc
    18601 accatcaagc cataattcta tataaatata accactgtcg gtgagaggct taatatgcat
    18661 acaaagctct tctaaatcat caagagatat tggctccaaa aaatcgatcc tgacatacct
    18721 tattttttga tcataatcta atcgaaccga atgggctcca tgaaaaccat ccccaccaaa
    18781 tggttccccc ttgcctccga tatcatattt aaggccgtaa gacccagcag ccgccatgac
    18841 catcgaagag aggatcagaa ctaaatcgaa tatagttaga agtctcatgg tcgactattg
    18901 ggataactct agtataaaaa ctaatctgta tttgagagat cgtgtccccc taattcgtta
    18961 aaaatgacct atgttccatc caccgccttc tcgtccctcc atcccccgga cgcctatacg
    19021 agtttcacag gcggttcaca taaccgagac gagcaaaaat cgactaacag ggacccaaca
    19081 tgaaaaatag gcttttaaat ttcccaggcc aggcttggtt ggcgtatggt tcgctgaaga
    19141 tgcgctgaag atgtaggtga ttgagcatgg tagggggatt ggttacgact aaactgggag
    19201 aggagtccct gagaaggttg gggcgggagg cgctcgtcaa ggagaacctt ccttacgtca
    19261 tggggacgat ctcgctccta ttgatggttc tggctacggc ggtatcctcc gccaacgccg
    19321 ctggcatcgc gactattcag agcacggggt ccggcatgac ctattcatcc atctcttggt
    19381 ccggctacag ccttccgacc cccgttcctg atggggcata ttactatagg agtagcttcg
    19441 gaagctactt ctggccggag gtgcccccag aaggccgcga cccctggacc tcaggcctct
    19501 ggacgagccc gcggccgggc agaagctggt ataaccagca accagtggcc accgacctct
    19561 atccgaatgt cgcaggtccc gcgacggcag ggacggccgt cacctggact gccatggcga
    19621 cggatcccga tggtgacgcc atcctctaca agttctggct gaagggcccc tcgacgggct
    19681 gggcctggaa ggatatgaca ggatggacgg cgagcaacag gtggacctgg aggacggccc
    19741 cctcggatgc cgggacgagc tcggtctgcg tcagcgtcaa ggacgggcgg aacgcgggcc
    19801 ccgacggctc caagtgcagc ctgacaagag agttcagcgt cgccctcccc aggacgaacg
    19861 ggccccccac ggcgacgggc ctctccccca gccggggaag ccccgccctc ccgggcgcct
    19921 ccgtcacctg gacggctaca gctaccgacc ccgatgggga ccagatattc taccggttct
    19981 ggctgaaggg gccctctaca ggagacgcct ggagggagat gacgggatgg acggcgagca
    20041 acaggtggac ctggcagacc acctcctccg acctcgggac tagccaagtg aacgtctgga
    20101 tcagggacgg aaaccacgcc ggatacgaca gctgggacag ctaccaggtg agggacttcc
    20161 aggtggcggc ccccagagcg aacaggcccc cgacggcgac gggccttgaa ccgaactgga
    20221 gaagccccgt cgtcgcgggt tcggcggtca cctggacggc gaaagccgta gaccccgacg
    20281 gggaccagat attctaccgg ttctggctga gggggccctc tacaggagac gcctggaggg
    20341 agatgacggg atggacggcg agcaacaggt ggacctggca gacgacagcc tctgacgtgg
    20401 gaacgagcca ggttaacgtc tggatcaggg acggaaacca cgccggatac gacagctggg
    20461 acagctacca ggtgaaggag ttcaccataa cctggtgacc cctaagccgc cggagggatc
    20521 ggcagaatcc gctgatcctc ctaccttaca acgataagag aaagccggcg ctgggaggat
    20581 cttcgtctcc cagaatcgat atgatataga tcgccccaaa gggaggggtg gatcgatgaa
    20641 gggggggcgt tgggaggggg gctccctccc aactttccat caccttgacc ggcatctacc
    20701 ccggccagag gccgcctctt ttgtatcatc caggaccggc ccgcagatcc agagcttggc
    20761 ctgaagccac ttctccgccc ccgctggggg ttttcgcgcg agggacgccc tcagcccgat
    20821 ccgagacagt ttatggtggc gataccctct cctccgccga actgatcaga agatttaaga
    20881 ttaaagagca acaaccgatg aggaggggac tagattgcag atagagatca cagcactttt
    20941 ggatcaggac gtctacaccc agcgggggat cttcgtcgga agggtcgacg acgtggtcct
    21001 cgatccggat gtgggggagg tgagcggcct cgccctcggg aacgtcaaca ggtccttcct
    21061 cgacctgaag gggaggggca tcatcatacc ctaccggggg gtcaccgccg tcggggacat
    21121 cataatcacc aagcacttcg gcaccgtgcc cgtgccttac agcgatgaaa agaagggctg
    21181 agagccggga ggtttacgcc gacctccggg tccgggcccc cctggtggta cgggcagacg
    21241 gcagaggttt tggaaagctc ctccggaagg caaataagcc ctacgacctc gaattcgcga
    21301 gggcgatggc gggggccgca ggggcgttcg tcgaggagtc ggggctcgcc cccgccttcg
    21361 ccttcacctt ctccgacgag gtcagcatcc tctttctgga ggctcccttc aacggccggc
    21421 tggagaagat agactcggtg gtggcggggt ccctctcggg cctcctctcc atcggcctcg
    21481 gaaaggccgt ggccatggac gcaaggtcga tccccctctg tcccgaagag gtcgtcgagt
    21541 acttcgcagg aaggcaggac gaggcctgga gaaaccacgt cttctccagc ggcttctacg
    21601 ccctcatcga ggacggcctc gccccccgcg aggcgatggg ccgcctgcgg gggatgaagg
    21661 gggccgagat ccacgagatg ctctttcaca ggggcgctaa cctcgccaag agcccagcat
    21721 ggcagaggag ggggattctg atccgccggg tttgggtcgg tgacgagatc ggatacgcag
    21781 gcggcgaagg gacgggagaa gaggagaaga gggcgaggac gaagatcgtc gaggagtggg
    21841 agcctcccct cttccggtcg gaggagggaa ggcggatcct cgacgggaca attcggaatc
    21901 tttaggagct gggtatcatt ttaggagcgg aagatcatgt tcttggatga gaagggagag
    21961 gaagagggga agaaggatac ggcgaagccg aaacagttca tcccccgggc tttgggccag
    22021 aaggtgacga tcaggcttct ggacgggagg cccctgatag cgaagcttga ggcgcagaac
    22081 aactacgagc tcctcttgga cctggggaag gggaagaaga tgatagtctt caagcacgcc
    22141 atcggcaccc tggagtacga ggccctggat gagggtaggt gagatcggct accagccacc
    22201 cgcccgccgg agcctccggc cgagagcccc gggaatctca gaccacgcga cctcgtggac
    22261 gccctcgctg tcgtggacga tgcatctgcc gaaaggcgag ggctcgcccc tctcgaagtc
    22321 ttcatacttc aggagaagct cgatcgcctc ccggatctcg acgatctgcc ttcttttgag
    22381 aagctcggcc aatttcagag agtcctcggt cccgatctca cccccgagac cccggcactc
    22441 ggagcagcgg agacggccgt cctctaagta aaaagggtag gtctggcaga ggaggggcct
    22501 tccgccgtag atccggcacc cgctgttcga ataatatcgg cagttccggc cctcttttcg
    22561 gagccgccac tccagggtgt gaaagttccc ctcgcagtcc cactccccct cgaccggcgg
    22621 ctccgccgcc gcaagccacc ctccgcccgt atcggccgtg atcgtcctga cctcgaaggg
    22681 aaagacgacg acgctgttct cctcgccccg gcagcagtcg ccgcaacgcc ggcaacgaaa
    22741 gccgatccgc atgatctcgc gggcgatggt ctccgcggag aggctctcgg ccccctccag
    22801 ctggagcgcc agctcctcca gcatctcctc gatgccgctg ggaccggcgg acctctctat
    22861 ctccgacata cctcaaggaa gttttccagg agcttgatgc ccccttcggt gtggacgacc
    22921 tcggggtgcc actggactcc atagaggggc cgcttcttgt gccccatcgc ctcgatctcg
    22981 cagacctccg acctcgctaa aacccggaag tccggcggga gggagacgac ctggtcggcg
    23041 tgggaggccc aggtcttgat cctcggggcg aggcccttca ggatatcgtc ctcctccagc
    23101 acctcgacct ccacctcagc gtacccgccg acggcaccct tctcgaccct cccgccgaag
    23161 gtctcggcca tcacctggag gccgaggcag atgccgagga tggggatctc gagcctggtc
    23221 aggtactccg agcaccttcc ggcccgctca agggtggggc ctccgccgag gatgagcccg
    23281 tcggcctcga tctcctccgc aggagtctgg tttgagatca tcctcgccgg gacgaggtcc
    23341 cttatgctcc ggtggatcag gtggttgaac tgaccgtagt tgttcactac caggactttc
    23401 attcttgctc gccgtatctc gccatgagat gataaagctt acatcccgtt ttggggagag
    23461 gagacgagcc cttcgatatc ctcgggcccc gctaggacgg tgaccccggg gatcctcccg
    23521 agccgttcta tgttctctct gtccagggga cggctggcga cggagatcgc tccccttctt
    23581 atggcgccct gggggcagag gcccctgcaa atgccacagc cgtcgcacct caaaaggtcg
    23641 atctgctctc cgatcgcccc cctcgggcac gccgcccggg gctcgcaggc ctcgcagcgg
    23701 aggcatagat cccggtcgat ggtgaagggc ggctcggtct ccgccctctc caggacgtcg
    23761 gtggggagga cgtagacggg gacggccgcc ttattcgcca gggccgcggc gttcgtcggg
    23821 agggtgtcgg cgattccgca gacgatcttg gcgacggtgt tggccgtcgc cggcgcgacg
    23881 atcaagaggt cgaacttatt catcatgaac cgtccggcct tggggctgct cgacccctcc
    23941 tcccgctcca ggaagacctc ctccaggtat ccgccccccg atatctcttt gagccggtcg
    24001 aagaggccgt acatcctcaa cacctcctcg ccggcctcgg agacgaaggt gcagacccgg
    24061 ttctcctgcg agatcttccc catggcagcc acgctctcgg ccaggtagtg gccggccccc
    24121 gtgaccgccc aggcgacgtt cttcatagat cagatcaccc cacctatgag agaccaacgg
    24181 ctctggcggc ttatcacccc ggcggtcggc cgcctgtaac gaaaatatat aaaacatcat
    24241 cacaaatagt tgagaggggc tggccgcgat gtcagccctg gagagaacga accatgaccc
    24301 accacttcga aaaagccctc gtcggagagg cgctggtagg agaagggccc gaggtcgccc
    24361 acatagacct cgtcatcggg aggaagggaa gcgccgtcga agatgccttc gttgcggccc
    24421 tcgcctcgcc gaggatgggg cacacccccc tcctggccgt cctggagccg aacctcatcc
    24481 ccaagcccgc caccctcatg gtgaacaagg tgacgatcac caacgccacc caggcgcttc
    24541 tgatcttcgg cccggctcag gcggcggtgg ccaaggcggt gatggactcg gtggccgaag
    24601 ggactatccc ggaggctgat gccgaggatc tgctgatcat cgtctcagtc ttcctccagt
    24661 gggacgccga ggataagaaa aaagtcgccg attacaacta cagggcgacg aagctcgcca
    24721 tcgagcgggc gatgaaagga gagccgacgg tgaaagaggc cctagcgagg aaggactcgg
    24781 ctaagcatcc cttcgcctga gatggcttgg gatttttaaa atcggtcccc aggccgaggg
    24841 aaagctatcc cggtgccggt cgaaggtctg cggctggccc cggggaggct tttggcacca
    24901 tccacctccg ggggttctct ccatccgaca aactttactg tcaggagata ctaacttatc
    24961 cagttgtgcg tccatacgca atttacatcc agttcacgtg aaacggagtc tggagcattt
    25021 agcgccacga gactctgata tgaaaaatag gtacttaaaa tcccaaatcg acggattggt
    25081 cttgatacga atttgtcggt cgaagattgg aaggtgttcg atatggtaga tccctcatca
    25141 aagccggtcg cctgggagag acgggtcgat ctcccagggg atctcttgga acggagccga
    25201 actttagctc gtcgcctggc tctgtcgctg ctgatatttg gaatgatact cacttcggcg
    25261 gccttggcca acgttccacc gacggcctta gatctcaccc cagacaaggg gagcccgcag
    25321 gaggttggaa ccgccataaa atggaccgcg actgctacag atcccgatgg agatccaatc
    25381 agttacaggt tcttcctgaa ggggccgtcc acgggcgatg cgtggcagca ggtccaggac
    25441 tggtcgggga ccaacagttg ggactggacc cccacccagg atggaagcta cgaggtgaac
    25501 gtctggatca gggacgaatt caacgctgga cctgacagct gggactcatt catgatcacg
    25561 cccttctccg tgaacccggc ccccgtcccc aaccagccgc ccgtggctaa ctccctcaac
    25621 ccgaacgtcg caagccccca ggaccccggc acatccatca tatggaccgc cagcgcaacc
    25681 gatcccgatg gagacaccat cctctacaag ttcttcctca aggggcccgc cacatccaac
    25741 aactgggccg agacgagagg gtgggacccg tctccaacgt ggacctggac cacttctccc
    25801 gctgacgcgg gcgtaaatca ggtcaacgtg tgggtccggg acggaaacca tgaggggccc
    25861 ggtggatacg actccttcaa ggtcgcggac ttcgtcataa atgcgccaaa ccagccgcct
    25921 acggtcacta gcctgaaccc gaacccgacg agcccccaga acgccggaac tcc
//
LOCUS       DASM01000073            7178 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_105390, whole genome shotgun sequence.
ACCESSION   DASM01000073 DASM01000000
VERSION     DASM01000073.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_105390"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1396 a         2292 c         2214 g         1276 t
ORIGIN      
        1 ttcgtctcct ggtaccccga ctacggcccg gagcagaggg gagggacctc gaactgctcc
       61 gtcgtcatct ccggcgagag ggtaggctcg ccggtggtcg accggcccga cgtcctcgtc
      121 gccttcaacc ggccgtccct ggagaagttc gccgccgacg tcccgaaggg gggcctcatc
      181 ctctacgact ccctcgccgg agccttcgag cctccggagg gggtcaccgc catccccgtc
      241 ccggcgacgg agatagccct ctcccgaggg gtggagcagg cgaccaacac cgccatgttc
      301 ggggccctga tggagagagg aaacctgggg cttccccggg aggccttcgg cggggctttc
      361 gagaggacct tcgccaagaa gccgaagctg atacccctca acctggagat cctggaggcg
      421 ggggctatga gcgtcaggcc ggatctctga aggagccgca gatccgccgc ccgaccgaga
      481 agcccctttc tggaggggtt ccagggcggg cggatatctc ttcttcaacc tccccgagag
      541 gatcggcgtc ccctgccacc aaggaggaac ggcaggggat ctgaggggcg gtcgctccgc
      601 cggcgagtac caaaaaatac aaatatgacc gacgcctaac tatgattact tcaagcaatg
      661 aaggggtgag accataaaga aatttgctgc gctattagtc ttggtggcgg ctttcttcgc
      721 cgtcccagcc atggcagact gtaccgggcc tgactgcctc tgtggcttcc aggctgtgga
      781 cttctccttg ggcgagtggc ctgctaacgc gaacgtgaag ctcgtcagct caggagagca
      841 gcgtgccttt gccttttgga atggacaggc cttcaacgat ctgaaggttg agaacgacca
      901 gaatgcagga atgctgtatc aggaaggctt cccgaagccg gcgtgtgcct ccactttggg
      961 cacctgtacc gccgcggacc agatgggatg ggataccagg gtcgtaaaca tagagagagt
     1021 ctcctctggt aaccagttcg ctaccgccac cggtggagcg atcgccagaa acagcatatc
     1081 cgtttgcgct acccagggcg ctgctgcggg ctgtgattgc tgcgactgtg atacattcat
     1141 cgcccaatag gatacaatgg agataaaatg gcagctcatc agcctggcgc tgatgagcat
     1201 gcttctcatt tttggttctg ctgccgctgt agattgggac gcttcgatag cggagcggga
     1261 atatccagac tccttaaacc gggttagaaa ttttcacgat gacttcgcca ggggcggcac
     1321 ctccgccaag gtcggaacca cgaccctgag cgatttgacc aagggcagca ccatcggcac
     1381 ctccaccatc caggatctga ccggattctc catcttcgac gatgctgatg atgatgagac
     1441 cttccagggc gtagtggcca tcaacaccga gaggggaagg accgtcaccg gccggggaga
     1501 gttcggatcg gtcccgacgg acgtcaacta cctggacatc gacgtcagaa acatcatcgt
     1561 catatccata aacaccgcca ggggcggaaa cgccatcgcg acctccgaga tcgtcatcaa
     1621 gccgacccag cagtccggct tccggtagac ccgacgccgc cgcaggttct tctagtcttc
     1681 ctcttattgc atcccttctg cgcaggagag cctctgccgt cggcctcgga tggagcggtt
     1741 atgagatgcg atctcattta tttcccggat cgccacaccc gtcctctacc gccctcctga
     1801 gggcccgacc gagggcggtg gcgagggcct ccaccttctg ccagtcgtcg cctcccgagg
     1861 ccctcagatg gagggtgagc ccgccgttgt aggcgagggt tctcatgaag tggccgaggt
     1921 tctcaaggtc catcccctcc agggatcgcc gagatatgtc gacctcctcc cggtaacccg
     1981 ttgccccgaa cctgacggca gcgaaggcga gggcctcgcc ggcggggacg gaggagctcc
     2041 cgatccccgc caccgccacc tgggccaagg cctggccgaa ggagatggcg acgtcctcga
     2101 cggtgtggtg gtctccagtc gggaggtccc ctgaggccct cacccggagg tcgaaccccg
     2161 atcccttcgc caggagcctg aggatctcgt ccaggaggcg gacccccgtc tccacctctg
     2221 acgaccccga cccctcgagg tcgatggaga cctcgacctt cgtctccgcg gtatcccgcc
     2281 tgaatgaagc gcgcatggtg aggggtctat ccgaagatat tttaacttat tctgaggagc
     2341 cgccccggaa cccggaaggg cggtggcgat gtcgggtccc gagatctccc cgggaggatg
     2401 ggacggatcg aagcccttat gtacccctgg atcatgtcgt tccgttccaa aggttatcga
     2461 tatgatagat aagagcaaaa tacaagaaat tttggatgag tatgactcaa aagagctaac
     2521 tatagctaca gtgtgctctc atagcagcct ccagatattt catggggctc gcctggaggg
     2581 gttcaggacc ctgggaatat gcctcggcag gcctcctaag ttctacgacg ccttccccct
     2641 ggccaagccc gacaccttcc tcagcctcga tagctacggg gccctcctgg gggagagcga
     2701 ccggctcgtc gaggagaacg ccatcgtcgt cccccacggc tccctggtgg agtacctggg
     2761 gacggagagg ttcgaggaga tgccggtccc gaccttcggc aaccggaggt ccctagcctg
     2821 ggaaagcgac cgggagatgg agcgggagtg gctactcaag gcggggatag acctccctcg
     2881 gaggtttgcg acccccgagg agatcgatag acccgtcatc gtcaagtacc acggcgccaa
     2941 gggcggcaag ggcttcttca tatccaagag caagggcgaa tacctgagca aggtcgaccc
     3001 cacccaacgg cataccatcc aggagttcat cctgggaacc cggtactaca tccactactt
     3061 ctactcgccg atccggtccc acggctacac cctgagcaag gggaccctgg agatgctggg
     3121 aatcgaccgg cgggtcgaaa gcaacgccga cgagatcttc aggatcggct ccatggacga
     3181 gctggaggcg gcggggatat ttccaagctt cgtcgtcacc gggaacatac ccctcgtcct
     3241 gagggagtcc ctcctcccca aggccttcga cctgggggag agggtcgtcg aggcgtcttt
     3301 agatctcttc ggagggatga taggcccctt ctgcctggag acgatcgtca ccgacgacct
     3361 gaagttcaag gtcttcgaga tctcggcccg gatcgtcgcc gggacgaacg tcttcatcag
     3421 cggctccccc tactccgacc tgatcgagag ggacctctcc aacgggaaga ggatagccca
     3481 ggagatcagc ctcgccaggg accaggggag gctctcggag atcataagct agcgggcgag
     3541 ggcaaagttt tattatcttc cccccgcctg accttttccc ttatatggca ggtctcttcg
     3601 acacgtataa agacgccgcc gccctccagt gctccttcgt cgagaagctg acgaggctgg
     3661 acctggagag cgtaaacggc ttcgccgatt cgctggcgtc gtttttgagg tcaaaggacg
     3721 agaaaaactt cgtctacgcc ctcgcggcgg ggaggagcgc cctctccctc tacagcttcc
     3781 tccagctcct ctacaaccac ttcaacgcct tcccctgcac catggaggac ccggtccaga
     3841 agcacctccg gagcaccagg aagaacctgg ggatcgcggt ctcggggtcg ggggagaccc
     3901 cggcggtgaa caagtacatc gaggacataa taaaccaggg gggggatatc accctgatca
     3961 ccgccaacga ggatggcacc gcccatcggc tggtggagag gtacgagaac ggatcgatcc
     4021 tggtgatcaa ggggaagacg aagtacgacc tggacctgac caggacctcc aagctctctc
     4081 ccatggggac ggagttcgag ctggaggtcc tcgccttcct caacgccgtc ttcaaggagg
     4141 tccaaagcag gatcgaaggc gaggagagcg gagactgcca gaggtatctc tccaccatca
     4201 aggactttgc aggccagtgc catctcatct caaaggtgga cgaggggagg ctcacccggt
     4261 ggtttgagag gctcttcccc cggagcggca ggtacatcat cggcggcgtg gggaggtcgg
     4321 ggctggtggc ccgggccttc gagatgaggt tcacccacct caacaagacc agctactggg
     4381 agagggactt caacaccccc agctttcaga tcggggacgt ctacctcccc atcaccggaa
     4441 gcgggaacac ctacgagtgc ctcgaagggg tgaggagcgc cttcaagagg ggggcggacg
     4501 tccttccgat cacgactaac ccctcctcgg agctggtgga gctgatggcc gaggggggga
     4561 gggaggagga tatcgccttc atacccgtct ccgaggagta cctcgaccag atctgcggaa
     4621 gcaggaggac gacgagctgg ctcgtctcgg agtacagcat gcacaggtac ccgatattcg
     4681 agatcaacgc ctcgatcttc accaacagcg tcgtcgccgt cggcgccgag ttcctgggga
     4741 tcgaagaggc ggggatgaaa aggctccacg catgaagaag atcctggcga tctctgacac
     4801 ccatctccag gagggggcgg ggttgcccga gcccctcatc gacctcgcga gaggggccga
     4861 cctggtcctc cacaccgggg acttcgtcag cctcgaggcg tacgaggcct tcagagacct
     4921 cgcccccttc gaggcggtct cgggcaactc cgacgatccg gcggtggcgg ccctccttcc
     4981 tcagaggagg gtggtggagg tggaggaggt gaggatcggc ctcgtccacc ggccctcctt
     5041 ctctgccgac ctcctgggcg ccgacctcct ggcgagggag atggaggtgg acgccctcgt
     5101 cttcggccat ctccataggc ccctcctgga gggggtagac cgcctcctca tctgccccgg
     5161 aagccccacg gagccccgcc tatcgccgcc tgcggtggcg gtgctggagg tggagggggg
     5221 gggagtccgg gggaggatcg tccccctggg aaggccggtc tgcggctact tcaagttcgc
     5281 ggaggccctc gcggagaggt cggtccggag gggcggataa agaccccagc cctcgcggag
     5341 cgcccccctc tggcgatcgg cccgaaaccc aagaaccatt tcagttcccc ttctacttcc
     5401 gccgccgatg gaaggcctcc aggtcgtcga tggcgatcca gcccgaggcc ccctcatccc
     5461 catcgccgat ggagaccagg aagcggaatt ctatgacgtt cccgatctcc tcccagatcc
     5521 cctccgcccc agcccccccg aactcctcgt atcgagcctc cagccgggcc cattcccccg
     5581 ggactgcgcc ctcatcccat aggcggcgat agatgctgcc gtttccgtcg acgatcatca
     5641 cctcgaaggc ggtggggtct ccgccgcccc ggaggtggaa ggataggcca tcggttccgg
     5701 cgaggttgag gccgccctcc cctgccgtcg acgagaagac cttggcgatt ccagccaccc
     5761 ctccagggga gaggtcgaag gagatcctgg tggcgtcatc caccaccccc gggaccgccc
     5821 tgatccccga gatggcagag cccctgccgt cctcgtaggt gctccacccc ccggccatag
     5881 agtcgatggc gaggggggag aggaccgtga cgtccagaga ggagctgaca gaacctgcaa
     5941 agtttcggcc ggtgagggtg taggtgacgt tctcccgagg cgccaatctc acctcccccc
     6001 ggagggggac ggggcccacc cccggcccca tctcggccac cgtggcgttc tcgacggacc
     6061 atctgagggt cacctcctcg cccagatcca ggatggaggc atccgcctcg aagagctcga
     6121 tctcggggag aggccgggtg gtgaaggtcc atacctctcc ctgggcccgc tttttgattc
     6181 ggttctcggc caccacctgc catcggtagg cggtgccggg ggcgaggggg gccgtcaggt
     6241 tgatggatag gctctccatc tccaggccgc cgcgggccct ccccaccggc gtcatatcct
     6301 cgccggcggc cccgagaagg agctggtaat cgatccggga gttggggtca agacctattg
     6361 acgacaggac cggcgtctcc ggaccgcccc cgacccacca gagagggggg gatagcccta
     6421 tgccggcggt cttctccggg ggacaggggt tccccggctc agagggggtc ggcgccaggg
     6481 cgtaccccag gaggaggaag aaaagaaccg ccgcgatccc ccacctcgac cgcctccccc
     6541 gaagtagccc gtctaccttc tcggcggccg ccattagggc ttcaaaggaa ccgtcaatga
     6601 ggccggtcat acctcgtcct ccatcctgac catctcctcc ggtctcctct gatcagatcg
     6661 gcctcggatg ggtatatact ttcccagcca cggaggctgg atctgacccg cgataccccc
     6721 gacaaacccg cgggaggggt acgatcctga ggaagggcgg ggcgggactc catcggggat
     6781 gtattttata tctccggatc catatctcca tgatctaatg ccggtggctg gagagaattg
     6841 agcttcaatc gatgggattt gaaggtggcc cttataatcc tgtatctggc agttatctcg
     6901 ggcacgtcta gcggtaccga ggtgaacgac ctctggctcc tcatatccag ctacgaggat
     6961 atagggatta cagtccacga cctcgccttc ttcctcgcga cccacggata caactccacc
     7021 cccatgagga gctacgtgat ggtgaccttc tacgacggcg aaaaggccta cctcaccccc
     7081 aacggcgcag ctcccaggct cgccgacctc tggtcggagc cgccgacccc cacgggaccg
     7141 gtgagggtga tcctccccga cgccatccag aggaacgc
//
LOCUS       DASM01000074           14349 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_74838, whole genome shotgun sequence.
ACCESSION   DASM01000074 DASM01000000
VERSION     DASM01000074.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 14349)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14349)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14349
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_74838"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    13818..13885
                     /estimated_length=68
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2751 a         4604 c         4562 g         2364 t
ORIGIN      
        1 tcagggacgg aaaacacgcg gcccctgaca gctgggacgc cttcaaggtc acgcccttct
       61 ccgtgaaccc ggctccggct ccaaacctgc ctcccaaggt cgatagcctc atcccggaca
      121 aggcgagccc ccagagcgcc gggacgacgg tagtctggac cgccagctcg acggataccg
      181 atggcgacac cgtattctac aggttcttcc tcaaggggcc tggcacgtct aacaactggg
      241 tcgagaagag gggctggagc aacaccccca cctggtcctg gacgaccacg gccgccgacg
      301 agggaaataa ccaggtgaac gtctggatca gggaccaaaa ccacgcggcc gctgacagct
      361 gggacgacta caaagtggta gactacacgg tgacggcacc aaaccaaatc ccggtcttca
      421 actcgctgac gcccaacccg ccgagccccc agaacgtcgg ggcgacgatc aggtggaccg
      481 ccgacgccac cgacccgaac ccgggggacg ccgtccagtt caagttccac ctgaggggac
      541 catcgacggg aggcgcatgg gaggtcgtcc aggaatggag caccaagaac tggtgggact
      601 ggaagccttc ccagcagggc gactacgagg taaacgtatg gataagggac ggaaagcacg
      661 cgggacctga cagctgggac gcgtacaaga tcaccccctt caaggtgaac cctgcgccca
      721 cgcccaacag gcccccgacg gtcacggccc tcacccccaa ccggccgagc ccccagatcg
      781 gcggcgcgag gataacctgg acggccaccg ccagtgatcc ggacggtgac cccatcctct
      841 atcggttctg gatgaggggc ccctccactg ggaacgtctg gaaggtagtc agggactggt
      901 ccaccagcag gacctggacc tggtcaacct ccccggggga gatcggacag tacgacttct
      961 gcgtctacgt cagggacggc aagcaccagc cagaaggccg ctacgacgac tgcacggcgt
     1021 acaggggcta ccacctgatc gccatcaggc cgcccaacca gcctccagtg gccaccgccc
     1081 tcctgccgag ctggtcgagc ccccaggtgg cgggatcgac gatcacctgg acggccttcg
     1141 ccgcggaccc ggatatggac ctcctcttct tcaagttctg gctgaggggg cccgccaccg
     1201 ggaacgtctg gaaggtggtg agggactggt ccaccagcaa catctggacc tggtcgacgc
     1261 accccggcga cgcaggcccc tatgacgtct acgtctacgt ccgggacgga aggcacagcc
     1321 caccaaccag ttacgacagc tccaaaggat atggtggata cctgctgacg gcccccgtcc
     1381 ttgtaaaccg tcccccggtg gtgacagccc tcgcccccag caggccgagc ccccagaccg
     1441 ccggcgcgag gataacctgg acggccaccg ccagcgatcc ggatggcgat cccatcctct
     1501 acaagttttg gctgaggggg cccgccaccg ggaacgcctg gaaggtggtc caggactggt
     1561 cgacgagccg gacctggggc tggacctcga cccccgggga cgtcggcaat tatgacgtct
     1621 acgtctacgt ccgggacgga aaacacgagc ctgccacccg gtacgacagc gccgtgggct
     1681 ccagcggcta tcagctgacc ccgcccgtga cggtaaacag gcctccgacg gcgaccgccc
     1741 tttcgcccag caggccgagc ccgcagacgg cgggatcgac gataatttgg acggcgacgg
     1801 cctccgatcc ggaaggtgac cccatcctct atcggttctg gctgaagggc cccgccaccg
     1861 ggaacgcctg gaaggtggtc caggactggt cgacgagccg cgcctggacg tggacgtcgg
     1921 ctcccgccga cgccggcgaa tatagcctct acgtctacgt ccgggacgga aaacacgagc
     1981 ctgccacccg ttatgacagc gccgtgggat acagcggcta ccagctcatc agcccctggg
     2041 ggatctatca gctgacctcc ggcgacgcga tccaggacag gccctccctc gtcagctccg
     2101 ccgatggcca cctcttggcc taccagtcct gggaggcggg cccccgcttc aacggggaca
     2161 tattcctcaa gaggttcgac ctctattgga accagctcca gaaggtccgg gctacgaccg
     2221 atacggcata tcaggacacc ccgtcggcga tatatgccga cgggtactac tacgtagcct
     2281 acgtatctga cgaggcggga aactgggaca tcttcgtcaa gaagtacgac ggcggcctga
     2341 acctcgtcga gacgaggagg ctgaccacct ccccggcgga ccaggatagc ccatccctaa
     2401 tccgggcggg gagggacttc tacctcgcct accagtcctg ggagacgggg aagtcctcca
     2461 gcggggatat cttcatcgag cggttttcct cctcctggac ctcgatccgg aaggtgaggg
     2521 tgaccaccga ggcgagctac caggaccggc cgtctctggc cctgggtggc gacgacagga
     2581 tatatgtcgc ctacgcctcc gaggagtcag gaaacctgga catcttcgtc aggaagtacg
     2641 accgaagcct gaaccccctc gagccgaaga ggaggatcac caccagccca tcgagccagg
     2701 actacccatc cctcatcgcg cagagcggcg agttcaacct gatctacagc tccgatgaag
     2761 ttgggagcta cgacctatac ctggagagat acagccccag ctggaccctc atcgagaggg
     2821 cgagggtcac cgggaccccg ggggacgagg tctatccgtc cctctcctat agccccatcg
     2881 acggcctcct ctgggcggcc tacgtccttc aggacgggac cgggagcaac atctttgtcc
     2941 agccggcagc ctccctcgaa tcgatggcgg gctgctgggc gtccatggac ttcagcgcca
     3001 ccagggccaa cagcccctac accctcactg tgaagttcta cggaccctcg ggggcgctcg
     3061 tcgacccctc cagcctgaag ctgacctgga accccgccga cgcccacctc accggaagca
     3121 ccctccggaa ggtctcgacg ggaagttaca gcctcgactc ccggttcgga tcggcgggtc
     3181 ccaagacctt caccgtcagc tccaccgtcg cagggtgcag cactgtgagc acggtgacgg
     3241 tcctggtggc ctgaacgggc ttggacccca gatctggaga ttggggcgga ggcggcatct
     3301 cttcgcctcc gatccaccct ctttttatcc ccgagcccca ggggcccgcc ctctggaggc
     3361 gtagcctcag cggggagacc ggtcgaagac cgtgacgtac agccgctcgt tataatcctc
     3421 gatcgtcgtg agggccttcc atccggcgta gaggtagagg gccaagatca gagggaccag
     3481 gaccaggctt gctgcctgga gagcctcgcc ggggacgaag ggtccgaatc cgcggaaggc
     3541 gaggagggct ccgaggagga ggatatcgac gaggaccagc atcagtccca ggacagccct
     3601 ccccccgtag atgagacctc cccccgcaaa gaggaggagg accatcaaaa gtcccaccac
     3661 aggcttcttc cgctcaagct cgtagaatag agctttctcc atatccggca tcctctgggc
     3721 gttctcccat atgatctgga acttctcttc ttctctcatg ctaaaggcct cggcattatt
     3781 gaggcaggtc gatttaaagg tttgaccatt tccacaacct gcgaaaagta tttttataac
     3841 attatgcgta actgatatat aactaaaata tatatcatcc aactggtcaa ataggtgagg
     3901 gagctatgtt agccctggca gccatcatcg tcggctctat cctgaccgga tactccgcat
     3961 acaagatcca cgccgcccgg aggggagaga gccgtaagct ccgggatatg gaggtgctga
     4021 ggcggcttaa cgtcccccca aaaaagcagg tccgggtctg gagcgccctg atggtcctgg
     4081 ggctcgtcct cctgacgcct ggcttggggc tcctcctctc cagcgatcgg ggctccggat
     4141 atgttggcaa cctctcccct tcgggcctga ggccttccac cccggggagc cccctcctgg
     4201 tccctccctc ggagaagctg gatatgaccc ccaagaccgt cgatgagggg cggtctgggg
     4261 ccttcggggg aggcgccgcc ttcgcctcgt cgggaggaag cggcaagagg gcatcttcag
     4321 ggggtggcgg aggatcttcc tccgccgagg cgaaggaggg ggtccgcgac gatctcggcg
     4381 gctcctcgaa gggggaaggt cccgaccatc acccccccga ggctaatgag accccgacga
     4441 tctacaaggc cggatccgcc cgcgatcgcc ccaggatcgg cgaggtaagc ccctccaata
     4501 ccaccgccgg accagaggcc ggggccttgc cggaggcccc ggcggctggg ccggagcctg
     4561 caggggagga agccgtcgcc atcgggccat ccgtggagac gacctctgaa ggggtggctg
     4621 aaaagaggcc ccttccggac ctttcgagac cagtggtggc cggatctgtg accccctccg
     4681 ccaggactgg ggagagagcc gaaccggagg ccttggatga agccccagag gagggcggag
     4741 gatccgatga ccccggccat ctggcagcgg gacccgcccc gaaatcggcc acctcctccg
     4801 aggtggccgg atctgccgcc atcgacgtcg ccagggagaa gaggaccgcc cccagcacat
     4861 cgaggcgtct cccctcggag gagaaggggg cggaggaggt cggtgacccg gagaaggaag
     4921 ggaccgctcc cccagccccg gcggaggcct cggggatgga gggcgaggcc gggcacctcg
     4981 tcgccggacc ggagccgacg tcacctcccc catcgccgga actggatatc tccgatgggt
     5041 ccctggagcc ccccggcctg gccgatttgg gtatggagct ggctcccgag gatcctgccc
     5101 cggtgatggg ggcccaggtg gaggaggtca agaagctcct cttccgggag gcgacccgat
     5161 cggatatgac ggtgggtgaa gccccctcag gggccgacct gataggcgtc gatggtgacg
     5221 gcgtgccgcc cgggaatatg acggtggtag ctaccccctc ggaggccgac gagctgaatg
     5281 gaagctccga tatcggctca aacctctcgg tggggaggat cgctatcgat gccgagttca
     5341 agaatttcgg atcggcgaat cggctgggga tggggataga tgagaacggg acccagagac
     5401 cggtgatgat aatggagttt gaaaagatcg atctggaatc gaacttccgc caggggttgg
     5461 ggttgacccc cacgagcggc ttccgctgat cgccgaggag ccgtcccggc ggggaagggt
     5521 caaggagatg aggatatatg gagcccgaaa cggtcgccgt cccagagggg ttcaaagttg
     5581 gatgccggat agcatccatc atcttattcc tcgtcctctt cctggttctg ggggcggtgg
     5641 gcgaggacct ctccgccaag gattggtcgg atatggggaa cgcccaggcc aggagggggg
     5701 agtacgagga ggcggttgcg agctacgacc gggctacggc cctggacgcc tacaaccccg
     5761 acatctggta caacaggggg ctcgccctct ccagcctcgg caggtacgaa gaggcgctgg
     5821 aatgctacca gcggggggcg aagatcgagc ccttcgaccc cgacctctgg ctcggccacg
     5881 gctccgccct ctccagcctc ggcaggtacg aagaggcgct ggagagctat aaccgggcgg
     5941 cggagttcga ctcgaaggac cccgacgcct ggaacgggag ggggacggtc ctcgcccgcc
     6001 tcgggcggct cgaggaggcc ctggcttcga cggataaggc cctggcgatg gacccggagg
     6061 acgccgacgc ctggaacagc aagggggcga tcctcctcca gctcggccgt cccgaggagg
     6121 ccctggcctg ctacgaccgg gcgatcgaga tcgaccccga ggatcccgac ctatggaaca
     6181 accaggggtc ggccctccac cagctcggca gatatcagga ggcccaggat agctacagct
     6241 gggcgatagc tatcgatccg ctccacgagt acgcctggta caaccggggg ctcctcgtcc
     6301 ccaccctaga tgaggagacg gaagaggcct tcgccctttc cagagagagg atgtacgtcg
     6361 agacggagaa gaggttccca cccacctcgg agggcggtgg atacgatgag acgaagaacg
     6421 agaagctttc gggctggagg accgtcgcgg tgatcgctgc gatattggcg gcagggatcc
     6481 tatcgaacct ggggggtaag ggcagggcgc tccatcgaga gccgctctcg tcgcccctca
     6541 acaggctgat aatcttcgct gacagatccc tgaggaggcg cggaccatga gaatcacgac
     6601 gaagtttgcc ataatgctga tcctggcggc ggctatcgcc tcggcagccc ccaacgaggc
     6661 gaacggcaca gtgatcgccg tcatcgccgg ggacgtcctg gaggtggcca tcggcggggg
     6721 agacccccgg acgggttctg ggatggtaga cggtgagagt cgcggacgtg gccctccccc
     6781 cccacggatc ggaggggtgg gaggtggcga ggacgtttgc cgaatccctc ctcaagaaca
     6841 ggacggtctc tctggacatc gacgaccgga ccgaaaccgg ccgggacccc caggggaggc
     6901 tcctagccgt cgtctacctc tctgacccca cgggagggtt gaacctcacc caccccctca
     6961 acaggctcct ggtggaggcg gggctcgccg aggtctgcga ccttgaagcc gacgagttcg
     7021 atccgagaga atggtggcag aggggcgagg cggacgccgc cggaggaggg gtcagggtcg
     7081 tgatcaacga ggtagaagcg aaccccccgg ggagcgacga gggaggagag tgggtggagc
     7141 tctacaacga cgggtttgag gaggccgcca tagggggctg gaacctgacg acggcggggg
     7201 ggtccgtcgt cggcatatct cccgggacga tcctcgttgg cgggggtttt ctgttggtga
     7261 aggccgacgg ttactggctc agaaacgatg acgagctcgt gaccctcaga gacagcgcag
     7321 ggatggaggt ggacagaacc ccggccctcg atgatgggga ggacgacaac aacagctggt
     7381 ccaggtaccc cgacggcggc gaagagtggg ccttcgtcca ggcgagcccg gcggggcccg
     7441 tcccggcggt ggagccgtcg gaggtgacca cctccgacca gaaggaagag gagaagaact
     7501 ggctcctggg gtcggggggc tgcgatcctt acggcctctg ggagatctcg gattttctcc
     7561 agtagacggc ggtggagggc gatcttctaa cgggcctcca ggtcccgggc cctatcgagg
     7621 gcctcttctg cttccccttc cctccccaag gacttcaggg cgagggccct gttccgccat
     7681 gcggtggtgt accccgggaa gagctccacc gccttcccga agcactccac cgcctcttcg
     7741 ggccgtttga gggcgatgag agagaccccc ttgttgttcc aggtgtagaa gtcgaagggg
     7801 tcgatgagga tcgactcgtc gtagcattcc agggcctcct cgtggcggcc gagattgtcg
     7861 agggctgccc ccttgttgtt ccacgcccgg ccagccctcg gccccagggg ttcgatctcg
     7921 agggccctat cgtaggattc ggccgcctcg gagaaacttc caagagcgtg gaaggcgtat
     7981 cccttgacga tccaggatcg ggcatctttg ggatcgagac caagggccct atccgccgac
     8041 tccagggcct cttcgtgccg gtcgagctcg ttcaaggcca ccgcccgccc tctccagctc
     8101 cgggggtggt ccggcttcaa atccagggca gcttcgtaag attcgatcgc cgcctggtag
     8161 cggcccatct tccgaagggc ctccccgagg aggtagcggg cctcgaagaa ggtggggtcg
     8221 gcctctatag ccctcgcaag actatctgcc gcctcttcga agaggcctga accatagtcg
     8281 gcggcccccc tcttgtacca gtagtcagga tccaacaagc cctcgccccc ggagccgacg
     8341 atgaggagac tctgatctcg gccctctcca gacctctcgc ccgccacgtt gttaagcctt
     8401 atctcatccc ctcctcatct catccccgga ggagatcctg gatccggtcg cggaagagga
     8461 cgaggccgtc ccggaggggt ggggccccga ccctccagat ctcctccgcc cgatctccga
     8521 gatcgatgag ggcaatccgg agggggccgg ctccagtccc gaagaagttc acgtagagga
     8581 gggcggcggc gaggacccca aggaggaccg cgaccttcgc ccgggatcga ctcctcctcc
     8641 ggccccgcag atcggagccg tcggcggcat cactccggcg gagcccccgc ttatcggcga
     8701 cgttcctgcg gagccggcgg atctcctcct cgccccagag cctcaccccc cgccggtcgg
     8761 cgaggtccac caccgacctc gcgaactccg aggagggaga taccacgacg gagatgcttc
     8821 cagggtgctt atccctggcc ctggcagccc tctcgaccgc cgcctcgtcg acggccgcgc
     8881 cggaaggctt gaggtggatg acgtaagtca cctggtggcc cacctcgtcg gtcctggagg
     8941 cgaagagctc cgacccgccg tcctcggacc tcgccgcctt ggcgactttg tagccgttca
     9001 gctcgatcag atcggagaac tcctcctcga acccggatcc ctccaccgat ggtctcaacc
     9061 gtcccgaccc cctctcatca tcctcgtccc atccctgagc tgttgcgata ttatttggcc
     9121 cattcatccg gagatctccc cgaaggggga tgttcagagc tccaaattgt tccaggagac
     9181 caatagctta tatattatga tattgtatgg ttatttatag ttggggcaga gttcggtctc
     9241 gccccggacc gtccccggat cggggctcct ccgccgcgat cctccggggg gccgaagggc
     9301 ggctccgtca cggcaagata tatattgcct ggcctcaatg cgctgtccgg atgtactcgg
     9361 acggtggtga tgttatatga gttcgatgca atctgagatc gccagccgtc ttgtccagtt
     9421 cctggacctg gacacggatg ggagaaggcg tttcgttctg ggagtgatcc tggaagcggg
     9481 cgaaatcaca gtaagctctc tatacaaggc ggtcaaagag cagtttgaga cctcacgcaa
     9541 ggtagtggcg tcgctgttgg gttatatctg ctcaaagctg ggaatcctca gcgcacataa
     9601 gaaatcctac cggctgccaa cggtttacgt cctgaaagag gagtacgctg acctggtcag
     9661 atccgtcctc aatgccaccc tcccggctcc cagcgattca tgacccacgt caagaacgct
     9721 ccgagaaccg caaccaccct gaagatcaga tccaagtcgg agtactggat cagccggtcg
     9781 aaagatcctt accttgagct gatgctccgc ctatttcagg acgagaagac ggcccgtcgg
     9841 ggcagggatt ttctgaacat ggtggaggag gggcagagga agggcgaacc cctcaaaacc
     9901 agggactgga agaggatgat ggaagacctc ggagtatccc ggtccgcctt ctacttcatg
     9961 agaaacaagc ttcttggggc tgggctgatc gccgtccgat cgggggagta caggctctcc
    10021 gggatgttca gccgggacct cgccgacatg tcccgctggt ggtggacggc ggtgatggaa
    10081 aacgaccccg aggcgctcta gacggcttcc aaggtcacag gacggcggag agctccccca
    10141 tcctcttctc cagggtcgtc acgatccgat cgttctccat gtactcgagc cccgcgccgc
    10201 agaaggggca gatgaagttg ttctcgctcg cctcatcgaa gacgaaacgg gcgcacccgc
    10261 caacgcaggc gtaaaatatg ttctccttct cataggcgag ccgctcctcg agcttcctca
    10321 aaagctttct cgcctccgac tccagggctc gatcgaggtt atcggagcat aaagtccata
    10381 ggtaggtgag ccagccgctt tcgggatccc tctcccgcct gtacttcgcc aggcggtgct
    10441 cgtagaggag gtagagggtc ctgcggactg tgttcaggct gatacccgtg atctccgcca
    10501 catattcatc ggttacctcc tccgccggca tgcagtcgac gatctggaga ccctcctccc
    10561 ccacgagctt tcgcaagtat gcgcgatata tgggatcctc aatcagtacc aataaaccac
    10621 cctgaaaccc gggggcgagg tcgcgcctcc gcagatcttc gcgccccgat taatttacca
    10681 tccttgttat tagtttatat gctgccattg cttataggga tttccctggc cggccgctct
    10741 cggttcgaat ggaaggccat tccgggggcg gtcgccgagg gggggtcaag ccgcccgacc
    10801 accgcccccg atcctctcga cgaaccgcct cttcttcgct atcgccctct ccgcggcccg
    10861 gtctaccttc tccctcatct cgaggaactc ccgggcaggc ccctcgtcca ccgccctctt
    10921 tatgggggtg taggccgact cccggaaccg gggctcgaag tccatcacct ccccatcggc
    10981 gtggagctcc accccccggc cctcctcgac ccggccgatg gcgtcgatct cgacgcctgc
    11041 gcccctcaca gcccggacga tctctccggc ggaggagggg ggggcgatga tgaggagggc
    11101 gtctatcgag accccgaggt agtcgatctc cagatcctcc agcatctcca ggacccgccg
    11161 gttgacgagc cgggccatcc tctcctcctg gaagatcagc ttaacccctg cggtcttcga
    11221 tatttctttc gcgtcgcccc tgatcccacc attggtgacg tcggtcatgg cgtggatctc
    11281 cacctcctgc tccaggagga cggaagccgc ctccaggaac tttatgttca tcgtctcatc
    11341 gacgacctca tggcggccgt agtagagggc tgcggcgcag accgtccccc cccccgcccc
    11401 ctccgtcatg aggatgacat ccccgggggc ggcgtccttc ctcggcgaga ggcggtcgga
    11461 gacccccacc gccccaacac agcccgtcag ccgatctccg atcaccatgt cccccccgat
    11521 ccgcaaggtg gagccggtga tgagggggac ccccgtcagc tctccgacgg cggcgatccc
    11581 cgccaggtgg tcgaagaggc ggctgaggtc gccgtcgtcg gccaagtgaa tgtctgagaa
    11641 catggcgacg gggcgggccc tcatgacgta tatgtcccgc agggtggccc tggtgacgtg
    11701 gaagccggcg aggaaggggt agtcgctgag gcgggagtgc atcccgtcga cggtgaggac
    11761 gatgtacctg ccgccggcct cgaccgcccc cgagtcgtcc agctgccggg agtcgacgac
    11821 ggcatccgtc tccccgatca cctcggcgat cttctcgtgg gcgtagaagt cgccgagccc
    11881 ccgggagccg acgccgaact cccccatggt gacccccgcc accggtaggt cgaatacgtc
    11941 accccggggg ctcaaagtcg ccgtcgcctc ctccagaacg gctttggcta gcctctccgc
    12001 gtgagccctg gttatcctct cccccttcac ctcgacgatc atctcggcca gcctctcctc
    12061 gaggtcgggg tggcccctcg ataggcccct cttggcaaaa ccctccaggt ccatggaggg
    12121 tggtggacgg cgggggataa aaaagctcca tagcctagag gcctccatcc agccttgaat
    12181 cccggccggg gatacaagaa tatatccaaa gaccgcgaga aagattgggc agatctgacg
    12241 attggtagga tctaagagga agagagcgcc ggaggaccat tccagggagg gttagtttga
    12301 ttccagatct gaagttcgtc cccaccacct gcccttactg cggcgtcggc tgcggccttc
    12361 tcctcgccgt ccgggatgag agggtggtgg gggtggagcc gtggcaggcc cacccgataa
    12421 acgaggggaa gctctgccag aaggggaggt acgcacacca gttcatccac agcgaggatc
    12481 ggctgaagac ccccctcata aagaggggag gctccttcgt ccccgccagc tgggacgagg
    12541 ccctccagct gatcgccagg aggctctcct cctacgggcc ggaggaggta ttcttcctcg
    12601 cctcggcgaa ggcgaccaac gaggagaact accttttcca gaagttcgcc cgggtggtga
    12661 tgaagacgaa caacgtagac cactgcgccc gcctctgcca ctcctccacc gtctccgccc
    12721 tggcgaaggc cttcggctcc ggagccatga ccaactcgat cctcgacctg gaggaggcga
    12781 ggtgcatctt cgtcatcggg agcaacacct ttgagcagca ccccctgatc ggaagaaggc
    12841 tagtcctggc gaggaggagg ggggcgaagg tcatctctgc cgacccgcgg ctgaccccca
    12901 cggcgaagga ggcggacctc cacctcccga tccgtccggg gacggacgtg gcgctgataa
    12961 acggcctcct ccgccggata atagaggggg ggtgggagga ccggggcttc atcgaggaga
    13021 ggacccgggg cttagaaacc ctgagggaga cggtgatgaa ggaggagtcc accctcccgg
    13081 ggacctcgag gatcaccggg gtccccgaag gcgatatcga ggcggcggca cagatgatag
    13141 cgacgctgaa gcccgccgcc ctcctttatt cgatgggggt gacccagcac acctccggcg
    13201 tcgagaacgt cctcgccctg gcgaacctga tgatgctcac cggaaacatc gggcggcccg
    13261 ggagcggggt caaccccctc cgggggcaga acaacgtcca gggggcctgc gacatgggag
    13321 cgctcccgaa cgtcttcagc gggtatcaga gcgtcgaaga ggacggatgc aggcggagga
    13381 tgaagagggt atggggggtc gaggagctgg gggaggcgag ggccgggatc acatcgacgg
    13441 agatgatgga gatcctggcg gatcggcccg ggaggatgaa ggccctctac ctgatggggg
    13501 agaacccgat gatctcggag gcggaccaga accgggtccg aagggccctc gataacgtgg
    13561 agttcatggt atcccaggag atcttcatga cggaaacaac agagctggcg gacgtcgtcc
    13621 tccccgccgc ctccttcgcc gagaaggacg ggaccttcac caacaccgac cggcgggtcc
    13681 agaggttgag gaaggcgata gatccgccgg gggaggcgat gccggactgg gagatcctct
    13741 cccgcctggc gagggcgatg gggtacggga aggagtttga ttaccggagg gcggaggaga
    13801 tcttcgagga gatcgccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnncacgc cctccagtgg ccctgccccg gcccccacca
    13921 tcccgggact ccgatcctcc acgtcgagag gttcgccacc cccgacggcc tcggccactt
    13981 ctcccccgtc gagtggaggc ctccagcgga ggtgccttca gaggattacc ctctgatcct
    14041 gacgacggga aggtccctct ggcactggca cggcggatcg atgacccgtc gcacgaagag
    14101 cctcggcggc gaggtggaga caggctgggt ggagatgaac gaggaggacg cagaggagat
    14161 ggggatcggg gacggcgagg tcgtccgggt ctcgtcccgg cgggggaata tcgagcttcc
    14221 ggcgagggtg acctccgaga tcaagagagg ggtgatattc atccccttcc acttcgcgga
    14281 gtgccccgca aaccggttga cgaacgccgc cctcgacccc gtctcgaaga tccccgagtt
    14341 caaggcctg
//
LOCUS       DASM01000075            7656 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_190052, whole genome shotgun sequence.
ACCESSION   DASM01000075 DASM01000000
VERSION     DASM01000075.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_190052"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1458 a         2307 c         2325 g         1566 t
ORIGIN      
        1 ttgacgagct tctgcatcag ctccttcatc acctcggggt cgtcgttgat cccccggagg
       61 aggacggtct gggaccccag ggggatgccg gcgtccgcca ggagcttgag ggcccgctcg
      121 ctatcggggt tgatctccct ggggtgctcg aagtggaggt tcatgtagag ggggtggtac
      181 ttcttgagga ccttgacgag cctcggagtc acccgctccg gcatcaccga ggggacccgg
      241 gtcccaattc tgatgatctc gacgtggggt atcgacctca gctcccggag gatgatatcc
      301 agctcccggt tggaccgaag gagaggatcg ccgccgctga ggaggacgtc cctaacttcg
      361 gggtggcccc ggacgtactc gatcccctgg aagatctgct gcatgggggt ctgttcgacc
      421 cttccgacgg accgctttcg cgtacagaac ctgcagtaca tggcgcactt ggagctgatc
      481 atcaggagga cccggtcggg gtaccggtgg atgaggccgg ggaccttggt gtccctcccc
      541 tcggagaggg ggtctgccac gttccggaag tcggagagct cctccgcgga cgggaccgcc
      601 tgcttccaga tggggtcgcc cttctcctgg attaaggcga ggaaatggct gctgatcttc
      661 atcgggtaga cctttttcac ccggtcgatc tcctccatgt cgcctccgag gcgggccagg
      721 agctcgtgga cggtctcggc ccgttgtgga ggcttatcgt cggaggattc ggctataaaa
      781 tcggttttgc cgctggaaat ggaggctgaa tcaaacattt gcctcacctc tcctgaagag
      841 aggtgtgatc atggatatga ccggcttcat cggatctaat gctccgaatt cgtctatttt
      901 ctggctcaag ctggataata cctgctttga tggtctgaat ccaggtaata cccttgaatt
      961 ataactatca aggtggtttg cgaagtcatt cgcggtcatg tgtttaccaa cccgctgata
     1021 tctatatcag gtagataaac ctgttgatcc gagggtttga gcctcgaaaa aagccatatt
     1081 atccctggtt atcttatcaa aatccgaatt actcttttta tttggctttt aggcagaatg
     1141 tcccggaaat gctctttccc cgtcctatga cgctgtaatc gggcgtttaa tggactgaag
     1201 ccatgatcca ggggaataaa atctcagatc gtccttaaat agaaagatct gccctcccct
     1261 tccccggtcc gggaggggga ttacattcgc gaaataagag ccatgattcc ctcccccggc
     1321 gggctccgcc ggctcctcca tctcctcccc ttcggcggcc ccctgccctt ggacaaggtc
     1381 aggcctcgcc ttcttcgccg gcctcggaga tctgctggtg gtagaggcga tggccgcacc
     1441 gcctgcagta gaaggcgtcg acctcgtgct cctcaaggcc gcagaggggg cagagggtgc
     1501 tcgtcttcct ggtgatcagg acccactcct tgacgatctt gctcgcctcc caggggatga
     1561 ggatgatccc ggagaggatc atcaggatcg tcaccagtct tcctgcatcg gtggagggag
     1621 tgacgtcccc gaagccgacg gtcgtcaggg ccaccactgt gaagtagaag gcgtccccaa
     1681 agttggagac cccggggttc gtggggcttt cgacgtggaa gaagagcccc gagccgatga
     1741 agaagaggat caaaatcgtc gtcatcagcc ggaccagctt caccatctcc agggagatcc
     1801 tcccgaagag gaggtggggg acggcgagga accggaggaa cctgaagatc cttaagacct
     1861 tgaagacccg gatcgtcttg acgaaggcga tgttgagggt gaggccgaag agggggagga
     1921 ccgcctggaa gagggtgggg aggatcgcca cgaggtcgat gacgctgtag acgtctgaga
     1981 tctgccttcg ccgatcgggg gcggcgtaga ggcgggcgag gtactcgagg acgaagaggc
     2041 cgacgactgc gacctccagc ctccacagga aggacctcgt cccttcggag atctcgtagg
     2101 tgtcgaggac gaagatggcg cagacgagga ggttcaggaa gatgatggag acgtcgatcc
     2161 ccttgcccag gggggtctgg tagtcgatga ggtagaactc caccagctct cggaagttct
     2221 cggggcggtc ggggatgttc ctggccatct cagcatcctc ttatatcatc ctctggataa
     2281 aggaccttcg atcctcgatc cctgtccgga actgaggaaa tttcatataa gggggcgggg
     2341 ctgaaactcc cgggggggtt caagcgccat gagcagatcc gagaggactg agctgaagcg
     2401 ggaactgggg ctcttcgagg tgaccctctc cggggtcggg atcatcctgg gggccgggat
     2461 atacgcccta atcgggaagg cggcggagct tgccggaaac tccgtatgga tcgccttcgc
     2521 cctggcggcg ctgatcgcca tcttcaccgg cctcagctac gccgagctct cctcgatctt
     2581 ccccagggcc agcgccgagt acgagtatac ggaccgcgcc ttcggcaaaa gactcgcctt
     2641 cgtcgtcgga tggctgatca tcttcagcgg ggtcatcgga gcctccaccg tcgccctcgg
     2701 cttcggaggc tacttcgagg ccctgacggg agcgccgatc ctaccatcgg cggtcctgct
     2761 gatcctcgcc ctatcgatga tcctcctctg gggggtgaag ctctcggcct ggtttgccat
     2821 cgtattcacc ctcatcgaga cggcggggct tttgatcgtc atctacgtcg ggatcccgca
     2881 tctcgggagc gtcgactacc tccagatgcc cggcggcctc accggggtct tcgaggcggc
     2941 ggccctcatc ttcttcgcct atatgggatt tgaggagatg gtgaagctct cggaggagac
     3001 gaaaaacccc gagaggaata tgcccctcgc cctgatcgct gccctcgcca tcaccatcat
     3061 cctctacatc ctggtggccc tcagcgtcgt ctccgtcgtc ccctgggagg ttttggccgc
     3121 ctcatctgcc cccttcgccg aggtggcggc cgaggccctg gggccggagg ccttcgtcgt
     3181 catctcggcc atcgccttat tcgccaccgc caacaccgtc cttctgatgc tcctcgccgc
     3241 atccaggata gcttacggga tggccgaggc cgcgtcgttg ccgaccgtcc tcggccgggt
     3301 ccatccgggg aggaggacac cgatggcggc gatcgtcgcc gtcacgctcc tctccatcgc
     3361 cttcgccttc tccggggaga tcgccttcgt cgccagcgcc aacgacttca tcctctttct
     3421 gaccttcatc gtcatcaacg gaagcctgat agccctgagg cggagagatc ccgaccggcc
     3481 ccgccccttc cgggtccctc tggccgtcgg ctgggtcccc gtcctcccgg tcctggggat
     3541 cctgacctgc ctcttcctca tcctccacct ggacggggcg gcgatcctcc tggggtctgg
     3601 gcttgccgcc gccggtgggg tcatagccct ggcccgccgc cgaagggccg cctgacccct
     3661 cgtccaggcg gcccgacctc gggggagccc caggcccccg cccgcggacg atctcgccga
     3721 ggccgagcct ctcggcggtc ggctcgatgg cgcagcggat ccacttgggt ccgacgggct
     3781 ttccgcacct cccctcgaag aggtaggttt tgccggggtt cttcttgcca tattcggacg
     3841 ccgccgccag aagctctggg gataggggga cctcctcgac ctccgaatcg ataacctccc
     3901 ccaacgatcc cgccatccct cctccggccc cctcgccaga cctcccccga agatccctgc
     3961 tcccgcgcct gccgagccgg agggcccctc ggcgggggtc gaggtcggag aacctcgccc
     4021 ccaggagatc ttggaggggg cggcccgtct ccaggaggat ccggagcatg atcctgtccc
     4081 cctcagagca gagggcggag aggagccgtt cccgctcctc gggggtcaga cgagggcttc
     4141 cagtggttcc ggcggaggtc acgatctccc ctgtatcccg gtcgaggtta aagtttttca
     4201 tttggaccct cctaaaccag agaggggtga tctccatcct cttcgaatga tgacggtgag
     4261 gctccctccc caaggtcgga ggcctctggc cgggtttcca tcccctcgcc ctcaggtcct
     4321 ttaatgtgga ggtgggcgga gtttagcctc atacttaatc ggaaaatact taatgatgta
     4381 atggtcttaa tatttagcat gggaattccg tgggcaggat ttggtctcat cccggagatc
     4441 ttgatctccg ttctgattta cttcgcggcc gtctccaacg gggtccagag tctgagcaac
     4501 gccgccgaag ccaccatctc ccagatcaga gtttccatga agacctgggg gatatcaggg
     4561 agcatcatcg ccctggcaga gagctatccg gctctcaaga cccaagagac ggtgatggtc
     4621 accatgataa tgatccgaga ggaggaccag ctgaagcggc ggggtcatct gaccagcgaa
     4681 gatgagcacc caagccggcc ggaggtgaga cggagatgag agaggagagg cacatcatag
     4741 ttctgataac ttcggtcttt ctgatcggag ccgtcgggct cctcctgaca gatggtatga
     4801 tcggattcca ttcgagagac accgaaaata tatcgctaaa acccctctcg atccccgatg
     4861 acgtctacgt ctatcaatac agcgcggact tctacctgaa cggaaccctc gaagagcgtt
     4921 ttatctacca gatatcaact tcgggaaagt acaggatgct ttatagaaat ttaaatgaac
     4981 gtctagcccg cggaaagctc agtatgccat ccttcgagct ggtggaggtc cggcccccgg
     5041 cgggaaccac cgcttatctt aaagactggt ctggcagggt ggagatcata tcaggagacg
     5101 cctccagggc ttcggagatc caatcccttg cgatgacaaa tgaggttgga tgctacctgc
     5161 cggagaggtt ttctcagggt cgatacgaga tgagctacct ctttaaagtt caccctcccc
     5221 tggagtgcga cgaagaatac tgccacctga atctgaagct ggccgacgag catctgccgt
     5281 accggacggc caccataacc atccacgacg agaacggctc tgtccgtcgg atattccccc
     5341 atccgccgat gcaagtcgtc caggaagggg atacctggat cttgagcgga tcaagcccca
     5401 aagatgagct tttggaggtg gagatcctct tcgccccgga ggtcgccggg gctatgggtg
     5461 gctttccgcg aaacgtcacc gaagttggca ataagacctt cgccgcgaac aatgactttc
     5521 acgggggtgt agactgggat actatcttgg accgcggctt gaccgggctg atactcctcc
     5581 tcccagcggg actatttctg atccaccgca gatatgggtg ggagaaggac tacaccgttc
     5641 ccgagaccct gagcctcgtc ccggagaaga ggaagccctg gctggtgaac ctggtcttca
     5701 acggcgactc ctttgacttc aacgagaacg ggttttacgc cacccttctc gacttccagc
     5761 gtcgcggcat aatagatatc gaatccgaag aggtcaagga gagcggatcc ctcttcagat
     5821 ccgagaagtc cgatttgagg atcaagcttc tgaagggccc cgaggagctg gacgacgatt
     5881 acgagaaaga tgttctgagt tttctcaatg cctattcttc aggaagcgtt ttcagcacgg
     5941 cgacatttga ggagatcctc aagaggatga gaacggatgc gaagaacgat ccttcaagcc
     6001 tgaacttcat ccgggatagg atgaagcggc tgatgaacgt tccaaacaag gatgtgtgcg
     6061 gaagcttcgt tgccagaccg aagttgtgcc cgcgatcctt cataaccgaa tccggtcgat
     6121 gggtcagagg gtggagagtt aatgcatatt tcgcaatttt attagctttt ttcatatttt
     6181 ttgatatcat catccacgga atcgccggaa tttttcggtc tccgatgacc accaagtttc
     6241 tgatcctggc acttcagctg gtaccggtgg cgatggcacc ctcatctctt tttggaagat
     6301 ggaaggagga ttactacaaa gagaagctgg agtgggatgc cttccgaaga cacctgacgg
     6361 actttgtctc gatccagaag tacgcccccg atgatctgaa gatgtgggag gagtggctgg
     6421 tctatggaac cgccctgggg gtgggcgata aggtcgtgga ggccatgagg gctttgaacg
     6481 tagaaattcc ggagatccga ccttccagcg atatgcccga agccttcagt cgctcttatc
     6541 gattgacgct acctcccagc actagttcag tcgggtctga tggcggcgga tttggtggag
     6601 gggggttcgg tggcggtggt ggctttggtg gcggcggagg cggcgccagg tgaagccttc
     6661 ctggaggtgg tttagccggg agccggggtt ctgacagcct ccggatcgat ggccagcccc
     6721 tcttccgggt ggaggcagga gtactggagg ctcgtctcgt agccgacccc atcgaaccac
     6781 tccgcccccg agacgatctc ccccagcccc cgatccccgg cagcctccgg gtccaccgcc
     6841 acgtgccccg accccggatg gcggacgatc acccacatcc gggggtggag gggatggccc
     6901 tcgtaacccg tcatcaggag ggcgtcgtgt ccctcccttt cgaggagcgc ccagcagacc
     6961 tgggccttcg cgaaggaacc tgggtctgcc ccatcggagg agtagctgaa ccggtgaagg
     7021 agcccctcca gccccaggat ccaggtgagg ttcacctcgg ccctggggtc gaggcggagg
     7081 agccggggcg ggtcgcagtc ctggacgacg agggcctcgc ttctttcgac ggcctccggc
     7141 agggcgacca ccagccccct gtaccatccg agatcctcgg ggccgatgag gctccccacc
     7201 ccgcgacccc ccgccccgga ggaggagatc gcctgccacc ccccttcggg ccctaggacc
     7261 accacccaga aggagccact tccccggagg acggcggcct ggtacccccg atcgaggagg
     7321 aggctttcga gggcctcctc ccttgccggg agggctgacc gggtcgatga gacgatctcc
     7381 tcggaggcga ggtggtcgac cctgtcggcc acccgatggg cggtcaacgt caccttcacc
     7441 tccacctccg gggcgggggc ggcctccacc ccctcgaagt gatgggatcg gagggtctcc
     7501 gagaggaccg acccccagcc gtcgaaggtg gcgtttctcg gctccagctc tacatatccc
     7561 tcggacccct cgtcccggaa gctctcgagg aacgcctcga acgcccccag gttctcctcc
     7621 cacccctcgg tgcaggacca gaaggaggcg aagaag
//
LOCUS       DASM01000076            2726 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_119550, whole genome shotgun sequence.
ACCESSION   DASM01000076 DASM01000000
VERSION     DASM01000076.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_119550"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          627 a          758 c          856 g          485 t
ORIGIN      
        1 cttcaccagg accttcacat cctatgaccc cctggatatc gccggcggga ggaacgttgc
       61 tgagggggcc gaaggctacg agatcaacgt cgccgtcgag cagatcatcg cctggaaccc
      121 cgactacatc ttcgtcgcct gcagcactcc ggaggacgcc gaggtgatcg actggatcaa
      181 gagctcacca gacctgcagt cgatagccgc ggtccagaac gggaacgtct acaactgctt
      241 ctacccccac tgccggggaa ggcccgccga tagaagcctc ctgaacatga tctacctggc
      301 caagcacctc caccccgaga agttccagga tgtggacctg gaggctgagg ggaacgagat
      361 cttcaaggcc ttcttggggg tcgacggcgt cttcaccgag tacgccgact acctggtctg
      421 gccgagggag gagctgaaga ccgagtagac cgatcctgga tgaagggcgg aggagagatc
      481 gcccctcatc ctttttatat attaaaatta atggagggaa ggcgctgacc ggattcataa
      541 actggaacga gcttcgaaag gcgatcatct ccgaggggcg aaggggaaag gggcgcgaaa
      601 gctccgaaga gatgtgggat aagagggcag aagagttcga caggtctacg aaggagagga
      661 gcgagaggac ggagaggcag gtggcaagcc tccagctcga tccggactgc accgtcctgg
      721 acgtcggcgc tggcaccggc cgcctctcgg tccccatcgc caagatggca aaaaatgtta
      781 cggccgtcga tcaatcgaga gggatgctcg attatctcga gaagaacatg gccgaggagg
      841 ggctctccaa ctaccggtgc ctccagaaga ggtgggagga cgtccggcta ggagaagacc
      901 tcgaggccca cgacgtggtc atagcatccc actccctggg gatgttcgac ctccaggagg
      961 ccctgacgaa gatggatgct gcagcgaaga ggcgggttta catcttcacc gctgctggca
     1021 agtggttctt cgatgacttc gaagaggagc tctgggagcg gatctacgat aggccgccga
     1081 ggcgaggcgg aggctttcgt tcagattaca tgctcctcta caacattctc cacgacatgg
     1141 ggatctacgc caacgtggag ataagagacg cagagcacgt tcagcgctac gggagcctcg
     1201 acgaggcggt cgagcggtgg aaggcgagga gggagatccc cgaggagagc gagccacttc
     1261 tcagggagta tctcgacaaa aatctagaag atgaaaacgg cagtggcctc accttcagac
     1321 ggaggacgaa gggcgccatg atatggtggt ccaagtcgga aagctcgtga tgcgttgaaa
     1381 ttcctttgat ctcaagcgag acgaaaatct agggggacaa aaaaagtgga atttgacatc
     1441 cggctcttgg caggattatt ggccataggc ctgatactat tttcatccat gaagatcaca
     1501 aggcagtacg agcgggccgt ggtatttcgg ttcggaaagc tcctggggga gaaggggccg
     1561 ggcctatttc tgataatacc gatcgtcgac aggattgtca aggtggacct gagggtccgg
     1621 gagctggacg tcccaagaca gacggtgatc tccagcgaca acgtcagcgt cgaggtggac
     1681 gccgtcatct actacaaggt gacgagctcg acgaaggcga tagtggaggt ggaggactac
     1741 gaggcggcta ccgctcttct cgcccagacg accctgaggg acgtcctcgg ccagaacgag
     1801 ctcgacacca tcctctccaa ccgggacgag ctgaacaaag aagtcaagat gattttggac
     1861 gagatgaccg acccctgggg gataaaggtc gtcaccgcca ccctgaggga cgtggccctc
     1921 cccgagaata tgctccgagc gatagcgagg caggcggagg cggagcggga gaagagggcg
     1981 aggatcatcc tcgccgaggg ggagtaccag gcctctcaga ggatgaacga tgctgccacc
     2041 ctctacgagg agaagccctc cgccatgaag ctgagggagt ttcagaccct caccgagatc
     2101 gcgaaggaga agaacctgat cgtggtatcg accggctccg acgcccccag gtcttcggcc
     2161 ggagagatct ctgctaccgt gggcctcgca aaggccgtcg ccgataggtg atgcaggggg
     2221 cggatatggc gaagacggtg aggcggatca caagtcgatc cggtaagaga agcaagatgc
     2281 aggaggccca cgactccttc gtattcgcta gatacttctt catggtggcg atcatcgcct
     2341 ccatcgtcct cctgacgggg gtgatcataa ccctggggcc cctggacatc accgtagccg
     2401 acgcctaccg cgccctcatc tccaagttct atccagacta cttcaacgtg gacccgctga
     2461 cctcgcgggt cgtctggaac ataaggtttc cgaggatcgt cggcgggatc ctcgccgggt
     2521 tcggcctcgg ggtatgcgga tgcgtcatgc aggcggtcct caaaaacccc ctcgccagcc
     2581 ccttcaccct gggaatatcc tcgggggccc agttcggcat atccatcgcc gccgtcctgg
     2641 gggtctctgt cgtcggcgga ccttacctcc tcataggaaa cgccttcctc ttcgccctcc
     2701 tatgctccgg cttcatcata gggctg
//
LOCUS       DASM01000077           22163 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_52488, whole genome shotgun sequence.
ACCESSION   DASM01000077 DASM01000000
VERSION     DASM01000077.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 22163)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22163)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22163
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_52488"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         4209 a         6870 c         6863 g         4221 t
ORIGIN      
        1 atcggctcgt cgatagatcc gttgatggat tcgttcatcg atccgtccat cggctcggtc
       61 gatcagatcc gttgatggat tcgttcatcg atccgttcat aagctccagg agggtttggt
      121 ctaggggttc ttcgggaacc tccagatctg ccagagtgac cctctctaca ttatatttga
      181 tcctaggatc ggatctctcc atcatgatct cgaaggtctt gccgtcgatg acggcgatga
      241 cggtcccctt cgcctcgtac ggcgatcccc aggctgtcgt cgggatcagt aaaagcagca
      301 acgccacaga tgcgatcttc ttcggcctca tcagcttccc ccttcaactt atcttcctcc
      361 cgattataag aaatatcttt cgacccgata tttcgccctc ttctttgtca aatccggacc
      421 ttccaccaga aggtggcttg agaaatggac cctttggggc tgagatcttt tgatagaggc
      481 cggatggccg gcccaccgag gcggccgggg cgttcgagag atccggggga tgagacctcc
      541 tgaggtcttc tccaaaagct ttaagtgata tctcttccat aactatccac gcaggtgaag
      601 gtcgccgacc agggtttcgc gccggatctc ttccggcatc tttatggcgg ctggggattg
      661 cgacatatac ggtgttggat gtggctgaga tggttgaaaa aatttatatg gaattcagga
      721 agatggagaa cgggaagaag gtggggatgg aatgcctcgg ccggcatttc gactacggcg
      781 accgggtgga gccctcggat cttgggttat ccatcaacgc ggccgaggag atggcggcga
      841 agtccaacgg gagcatcaag ataataaaga aaaaatagcc gaccctcctg gagaggcggc
      901 ggtgatgggg tcatcatctt ctagcgccgc ggcccgggac ggcgcgacct ctgcggggac
      961 ggggccgctc ccctcgccct cctcgggggc ctcttcgacc cccccggggc gggctcggac
     1021 gccgcgcccg aggcgttctt cgcctcctcg gaatggtatc gatgttccac ctctcggatc
     1081 ttcatccggg tgagcctctc gatatcccgg aggaagctcc gttcctcggc ggagcagaag
     1141 gagtacgcag ttccagctct ccccgccctg cccgtccggc cgatcctgtg gacgtagctt
     1201 tcgggctcgt tggggaggtc gtagtttatt acgtgggata tatcctcgac gtcgataccg
     1261 cgggcggcga tgtcggtcgc caccagcacc tgcaaccggc cggccttgaa gcccctcagg
     1321 gcggcaagcc gctggttctg ggttctgtcg ccgtggatgg cgtcggcccg gatcccgctt
     1381 ctggtgagga cctttgccac ccggtcggcc cgccgcttcg tcctggtgaa gacgaggacc
     1441 ctcttcagga acttctgctg gaggaggccg atgaggagcg ggttcttatc cttctggtcg
     1501 acgaagaata tccgctgctc tatactctcc accggcgtcg cctggggagc cacctcgacc
     1561 ctgacggggt ccttgaggag ccttccggcg agctcgccga tctcccgcga catggtggcg
     1621 gcgaagaaga gggagtggcg cttcttggga agggctgaga cgatcctatg gacgtctttt
     1681 gcgaagccca tgtccagcat cctgtccgcc tcgtcgagga cgaagaactc gatccccttg
     1741 aggtctatgt gcccctgctc catcaggtcc aggagccggc cgggggtggc gacgagggcc
     1801 tcgacgcctc gggaaagggc cttcacctgg ggctcctgag agactcctcc gaatacggcg
     1861 gcgtggctga actggaggaa ctggccgtag gtcccgaagg actcgtctat ctgggccgcc
     1921 agctcccggg tcggcgccag caccagggcc cggggggcgc ccgggacggt ccttctcctc
     1981 tcttcggaga tcctctggag gacgggcaaa acgaaggcag cggtcttgcc ggtcccggtc
     2041 tgggctatgc cgaggagatc ctcccccctc aggagatggg ggatcgcctc cgcctggatc
     2101 ggcgtcggat tggcatagcc ttctttcgta agagctcgct gtagcggctc tgcaatatcc
     2161 aaatcttcaa acagcattta tacctcttca ggaggagaga gcagggcgca ccatatgaat
     2221 taggggaaga aaagtccaca tccgtcggct cagatctgat ccgggagcca gactctggcg
     2281 gtctcttcaa gggcatatga gctttgacgt tccagctctg cagatcctgc agctgatgat
     2341 cattgtaaag ctgacagatg atccaacctc ccctagatcc cctcccccct atttaaatag
     2401 atggggttga agggggggga gggtccgctg cccttgccat cggcatcgat ttggagaaga
     2461 tcaggtcatc tcgcccaccc cggcggagag gagctcctcg aattcctcgt ccccctcaag
     2521 acacccacag ctgggtggcg ggaatcccaa atcatcatcc gcatcctccg agacctcctc
     2581 cgtcacctcc gaggaatccg gctcctcccc gatgcatcca caaacagctg cgaggatcaa
     2641 aagcccgaag aataaccccg gcctcaatcg gatcaccggt gtatctctat tcctctgacc
     2701 cgaataacct ttcggcgtct tccggctccg gggagacggg gtctggccga agttccgccc
     2761 ctcggccatt ctaccagagg agatgggggg cgaagaggag gagcaacccc aggattgcga
     2821 gaatcgtccc gccgaccacc ttgcaccatc tcgcgtacct ctcccctaag gagcttccag
     2881 cggcaaaggc cgccaggctg aagatcacca tgtcgtcgag catgaagaag aaggtgtaga
     2941 gggcgatgag gagatagttc tcgatggtgg aggtcccgct gagggcgagg atatgggtga
     3001 agacggcggg gatcgccgat gagcagagga actcgacggc gttgacgagg aaggcgaggg
     3061 cgacgatgga gaagaagatg gagacggaga ggggctggct tacgatcttt tctatccttc
     3121 ccatcgtctc tctccgcccc tctcccccgc cgaccctgca ggcggggctc cctcctgtgg
     3181 tgaggtggtc tctgatgctc aggatcccgc cgccgagggc gataaggccg atgaggatcg
     3241 tcaggatctg gacgtatccc aaaagaagga agacgtttat ccaggcggcc atgatgagaa
     3301 agtacagcgc ccccgaggcg aggacgaagc ttcccacgat cagccatgcc cttctccggt
     3361 ctctcacccc ggcgaggagg cctatcaggt agacgaggac ccacatggcg caggggttga
     3421 acccgtctac aagccccagg acgacggcga gggtcggaag cgagaacttc aagaggtcgg
     3481 tctcaccgag aagcgggagc tgaaaatggg cgctgtcaat ctccggttcc ggggctattc
     3541 cggcgccctt tatcccgtcg ccctcctcct cctccacccc ctccacccca tcctccccct
     3601 cagccgccct cttctcctcg cggatcgccg cgaggagctc ctcctccgta tggagcccca
     3661 ccaggggcct tcccccgacg aagatggtgg ggacggtcag gctctcgacg gcgatccccc
     3721 catcctgggc catctcgcgg aatagggcga acccttccgc cttcgagacg tcgacggaat
     3781 agaacgcagc ccccgggacc ccctccctct tcagcgcctc gaagatcggc ttctgctctc
     3841 cgcaggtggg gcaggtgggg aggtagaaga agtggatctc tatcgaggat gatgccgatg
     3901 gtcccgagcc ctccgccggc gccgtcggtc cagtctctcc cgcgagggcg taggagaggg
     3961 cgcccaggat caaaaggagc gccagaagcc cgatgttgaa cctcgacttg aagaagtccc
     4021 ctccgatgcc gcatatcctg accatcgtac cccatcccgg aaagctcgca tatcagcgat
     4081 cagcgagcag gtcgcctgtt ctctccttcc ccaggccgct ctcgcccttt acctttatcc
     4141 tcatcttatg agatacgttt tgctgatggc cgtgatcagc agggccagga agaccgaccc
     4201 caggagccgc tcgaatatca cccgccgggt gtgcctctcc gcctcgtatt cgattgaagg
     4261 gtaaaggaag gaggtcaggc cggaggtgaa ggtcgtcagg ctgaagagga aggggtctct
     4321 caccgctcga tcgcggccgg gatctcgctc gcctggacta cctcgcgaga atctgacgcg
     4381 ccctcagacg cgcccgctgc cagcaggagc gccatcagga tcaaagccgg aatcgatctc
     4441 gtcgggtcag cctcccggcg agtactttct tcattattgt aaaaaaagct tctgacgtta
     4501 agccgcagat tggaaggact tgagcattac tggtaaattg gcgctgaggg tgagatttga
     4561 actcacgagg cgcgtgagcg ccaccggttc ccccgtcgga ccgctcgctt tcgccgatgg
     4621 tccgagttca agaccggcgc cgtgggccgc ttggctacct cagcccgcgc ctctttcttg
     4681 gggccggtgc ttcttaaggc tggcgatctg ggcccctcgc aaaagtttta acgctgatgg
     4741 cggtagatga ggaaaccatg ctgatcagaa acgcctcgat cttcgcgggt ggaaggcttc
     4801 gccgggacgt gaacatcgcc atcgacggcg gccggatcgc cgatataggt cctggggtcg
     4861 cggacgagtc cggcgacggc gaggtgatcg agggcagggg gaagctcgcg atcccgggcc
     4921 tggtcaacgc ccacacccac ctcgccatga ccctctttcg ggggtacgcc gacgacatgg
     4981 agctgatgcc ctggctctcg gagaagatct ggcccctgga ggcgaagctg accgccgagg
     5041 acgtccgctg ggggacgaag ctggggtgcc tggagatgat cagatcgggg atcacctgct
     5101 acaacgacat gtactacttc atggacgaga cggcccgagc gacgaaggag atggggcttc
     5161 gggccgttct gtccggggtc ctcttcgaca tgaggcccga tctgatcgaa gaggccgagc
     5221 ccttcatccg gaggtggaag aacgacgacc tgatcgtccc cgccgtaggc ccccacgccg
     5281 tctacacctg ctcggaggag accctctctc gggccctgga cctcgccgaa aagcacgacg
     5341 tcatgatcca catccacctc tcggagacga ggtcggaggt ggagagcttc gccaagaacc
     5401 ggggcaaaag ccccgtcgag tatttagatt ctttaggctt tctcagcgac cgggtagtgg
     5461 cggcccactg cgtctggctc tcaccccagg acgtatccat aatcgccgac cggggggtga
     5521 acgtcgccca ctgctccatc agcaaccaca agctcgcctc ggggatcgcc cccctcgacc
     5581 ggctggcgcg ggcgggggcg aggatatgcc tggggaccga cggcgcctcc tccaacaaca
     5641 gcctaaacct cttccaggag atgaagacga cggccatcgc ccagaagtgc gcccactccc
     5701 gccccgacct cttcgccgct ggcgaggtct ggaggatggc gacggagaac gcctacaccg
     5761 ccttccgcct cgatatgggc ctccgggctg gggccatcgc cgacctctcc ctcattgacc
     5821 tcaaaaagcc ctggttctgc cccctcgccg acgacaacat cgtctcccac ctcgtctaca
     5881 gcgcccaggg cggcgtcgag accaccatcg tcggcggtcg ggtcctgatg aggggcgggg
     5941 tcataccggg ggaggaggag atcctggagc gggcccagga gcagttccgc gacctcctgg
     6001 cgaggtagat ggcctggtgg ccagcggcgc ggccccggat cggatcacca ccgtctcctc
     6061 ctccggatcc tggggaggcg gagccccctc ctcaccccgc cgaggtagat cagggttatg
     6121 aggatggcga cgatggggac gacgaagaga gcccgaagcc ccgtcggcgc cttcacctcg
     6181 atcagggcgg cgagctcctc cgacctctcc tcccccaact cgccggggag gtatcggacc
     6241 tgccagacca gggcccggtc ccccggatcg gccagcctgt ccacctccag ggggtactcg
     6301 gccgccgccc tcttccccgg ctcgatatct cccagctcca cctcctcgcc ggtggagttg
     6361 aagggggggt ggacgaggag cctagcccgg acttcccggg ccgggacgtc gccgccgttc
     6421 gccaccacca gctccagggc ggaggagcgg ccgggggata tggagccctt gatctccgcc
     6481 acctccagcc tcggccccgt catcaccggt acctcgatgg atatggtctc cgtagcctcg
     6541 gccctctgga agtggatctc ggggtagagg gggttacccc ggacgagaac gtcctccagc
     6601 ctctcgtaag acacctccag gagcatgggg tagaccccgg cgctggcgga gctctccgcc
     6661 cggaccgaga actccatcgg cctctctagg gcctcgcccc cggagaggga gccggccagc
     6721 tgggggcgcg agaggacctc cagcctcccg tccctggacg agagcttccc ctggatcccc
     6781 accgcctccg agccctccat ctccatctcc ccctccagaa gggctagcat cttctcgtcc
     6841 tcggtttgag gcgtcctctt ctccacgagg ccgctgacct cgccccggtt ctcgatcttc
     6901 acgaagaccg atcccgtctt ccccgtctcg aggctggagg tgacggaggt gacgaagatc
     6961 tggggtcctc cctcagccct atagaagtcg tcctccgccc aggcctgggg ggcgaggagg
     7021 aggaagaccg ctccgaccgc cgcgaggatg taggaggcga tggcagccct tcggagatcg
     7081 ccttttccgc cttctctctc tatcagcatc accttctttc tcatgtcgac ggtatcgaca
     7141 ccttcgatct cttatcctcc tccttgatag ctttcgtgat ataagcacgt ccctcggccg
     7201 aggcctggtc tctggaaccc ggggatgtga tcgatcggaa ctcgggggag gaagaatagg
     7261 caggaggatg ggaacgggat caggacgata gggaatggtc ggggctacct acccaaggca
     7321 tatcgccgca agggtccttt tagtagcttt tttagggatg ggtggcccgg ggcaggcggc
     7381 cgtcgtcccc ccaagtcctc cctatcttcc catctccagg gcctcctccg ccgtcatccc
     7441 cctgtggacg atggagcaga tctgcctggt gatgagggtc ggatccctgt gctggaagac
     7501 gttccgcccg atggctaccc cccgggctcc ggcgccgatc gccccggcga ccatctccaa
     7561 aaactccgcc tccgtctccg tccggggccc tccggcgatg accacgggga cggggcagcc
     7621 atccaccacc tcccggaagg agtcggggtc gccggagtag ctcgtcttga cgacgtccgc
     7681 ccccagctcc gcccccaccc tggcggcgtg ggcgatgtaa tcggcccggt gctcgtcgtc
     7741 gatcttcttc ccccgggggt acatcatggc gaggagcggc atcccccact cctggcaccg
     7801 ccgggcggtc tcccccaggg tcgccagcat cctcgcctcg tcctcggccc ccacgttcac
     7861 ctggatgctg accccgtcgg ctccgatctt cagagcctcc tccaccgtag ccaccaggac
     7921 cttgttgttg gggtcggggc cgaggttcgt cgaggccgag aggtggagga tcagacccag
     7981 gtcccggccg tacccccggt ggccgaaggt ggccgccccc ttgtggacga cggcggcgtc
     8041 ggcccccccc gcggcgacga ggtccgccgc ctccctgacg ttcttgatac cccggatcgg
     8101 cccgacggtg acgccgtggt ccatggggat gatcaccgtc ctatccgtct ccctgttgaa
     8161 gatcctctcc atcctgaccg actttcctat caaagccatc tcccctcatc ttgatgtatt
     8221 tatacgtaca ttaaagtact ttatcggcct ctgttggaca taatttttag tatttataag
     8281 ttcccgtcca tcgtctcgcc aggctgaaat accatcgatg tacctccctc cggatcgggg
     8341 agtgatcatc atcgagaata gtagatcgat tccaactcga gagggcggcg gccggatatc
     8401 gggggggctc ctcagatccg ccatctcgtt tatcatgttt ttcgcggtcc tcaccagcat
     8461 cagctctgct ggggggaata taaactgccc ggactgtcct gactggtcaa attttgatgc
     8521 ctggtgggcc aaataccata aaaaccctgc cgcagcggag ccctctaccc cccaggcggc
     8581 gcggactcag gctgtcgagg cgaggaacag ctctgaggag gtcgaggtgg agaggggaga
     8641 gtatcccgtg gcttcgatcc tgatccgtcc cggggacgac ctggagggaa gggtcctcct
     8701 ggacgcccgc tcccccgagg attatgagag ggggcacctt ccgggggcga ggagcctcta
     8761 ctggaggtcc ctcagggcgg gagacgtcct ggacctggag gtggcggcgg gagagctccg
     8821 gcggctgggg gtgaacgaga ccgactcgat cgtcgtctat gggagaggtg acgactcggc
     8881 ctacctcttc tgggccctcg attacctcgg ccaccaggac ctcagccttc tggacgggga
     8941 cctggaggcc ttccccgagc tcgggctggt ccagaactcc ccggagccgg cgccttccaa
     9001 ctacaccccc tccctgaggc cggagctcct ggtcaacgag tcgatcctca aggaggcgca
     9061 agggagcgtc agggttcaga tcgtcgacac caggtccagc tactccgacc gcgccgcatc
     9121 ccgcatatca aactccatgt acttcaagcc ggaggagatc tactccgacc ctgaggcccg
     9181 gaccctcaag agccccgagg agctcgatca gctcttcctc cgccgggggc tcgaagatcg
     9241 gatggttcag atcgtctacg ggaccccgga ggcctgcggc ttatacgtcg ctctgaaggc
     9301 gatggggtac cgggcgacgg tcctcgacgg cacctggtgg aagagtaccg agttcgccgt
     9361 cagctcccta tcctgaggct ccttgcgccc ctcttctcct agggtcgagg ggtaggaggc
     9421 tgcctcctgg ggatggaatg gcggaggaga gttcggggac gggaatccct ctgagggagg
     9481 agaggagaag gccggggctt cgagcccgag gccttctggt ctatgaggtt tttcaccttg
     9541 tccccagggg ccattccgac gagggctccg ggggctctgc ccttctctcg agagcagatg
     9601 taacttcctt cccgcggccc aggataaata aattgtggtt aactgaacgc aatattactt
     9661 gtttagaacc aaacacttaa tacgtattaa ttgcgaccgt atatccatgc ccaggcctga
     9721 acagatctcc ataaggagat gtatgccctc cgaggagctc gacgccagga taagggggct
     9781 ggagagatgc gtcggggtcc tgaggaggct gcacttcata agataccgtt accaggggct
     9841 cagcgttgag gcatcggccc acctcgtcgg cgtcaccagg agcgtaggat atacctggca
     9901 gaggaggtgg aacgacggcg gctatgaagg gctcaagccg aggcacggcg gcggaaggcc
     9961 tccgaagcta tcggcggcag agagagagac gctggcgatg ctccttcgtc agaggggccc
    10021 ctcgaggacg ggtgaggtga gagatctcct ccgaggcgag ttcggggtgg agtacacctc
    10081 gaaacagata cggataatcc tgaaggggct cggcgttgag accacccgga gccgttagaa
    10141 tgggacccgg atcagacggc ggatccttca ggggggggcc agaggcgggg ggcctggcga
    10201 cggcaaagga ggtcgtcctc cggaggccgg aggtctccga cggggcgaag atatgcgccc
    10261 tcgcgaagga gaccccgccc ctggataaaa actccgccta cagctacctc ctcctctgtc
    10321 gccacttcac cgatacctgc agcgtagccg aagaggacgg cgagatcctc ggcttcctca
    10381 ccggatatat tcctcccgat cgggtggggg tcttcttcgt atggcagttg gcagtggacc
    10441 ctcggaggag gggttgcggc ctcgggaaac tcctgatccg ggaggttctg tcgaggccgg
    10501 ccttgaatgg ctgcaggttt ctggagacga cgataacccc ctccaacgaa gcctctcgga
    10561 ggctctttct ctccctggcc agggatctgg aggcgagatg cagagtgacc tccttcttct
    10621 ccgaggagga ttttggtgga gaaaaccacg aagcggaaga tctcttccgc ataggaccgt
    10681 tacagcttca acgggtgata taattatgaa cgatatgagc accatcaatc gtcttgagtc
    10741 aaaggttcgg agctactgcc gccactttcc cgtggtcttc acaaaggcca ggggggcaac
    10801 cctggaggat gaggagggaa atgtgtacat cgactttctc gccggcgcag gagccctcaa
    10861 ctacggccac aacgacccgg ctctgaaggc gaagctcctc gattacatcg aggcggacca
    10921 gatagtccac ggcctggaca tggccacggg ggcgaagcag gccttcatgg aggccttcga
    10981 gtccctgatc ctctcgcccc ggaggatgag gtacaagatg cagtttacgg gcccgacggg
    11041 gacgaacgcc gttgaggcgg cgatgaagat agcccggaac ttcaccggcc gccagacgat
    11101 cgtctccttc accaacggct ttcacggcgt caccctgggg tcggtcgctg ccaccggaaa
    11161 cagccacttc cgggatgggt gtgggatgca gccccagggg acggccttca tgccctacga
    11221 cggctacatg ggcgccggaa tagacaccac cgagtacctg gacaagatgc tcgccgatgg
    11281 gagcagcggc ctcgaccatc cggcggctgt catcgtcgag acggtccagg gcgagggggg
    11341 gatcaacgtg gcgaggaatg aatggctccg ctccctggag gcggtctgcc ggaagcacgg
    11401 catcctcctg atcgtcgacg agatccaggt ggggtgcgga aggaccggga ccttcttcag
    11461 cttcgaggag gctgggatat ccccggatat cataaccctc tccaagtccc tcagcggcta
    11521 cggccttccc ctctccctgg tcctgatgaa gccggagctc gaccagtgga agccggggga
    11581 gcacaacggg accttccggg gaaacaacct cgccttcgtc accgccaggg ctgccctgga
    11641 ggagtactgg agggacggat ccttcgagag ggaggtgagg aggaagggcg ggatcgtccg
    11701 ggagaggctg gagaggctcg tcgacctgga cgagaggggc gtcctctccg cccgggggag
    11761 ggggatgatg caggccctcg actgcaccat cggcgaactt gccacccgga tttcggccct
    11821 cgccttcgag aaggggctga ttatggagac ctccggatcc gatgaccagg tcttaaagtg
    11881 cctcatccct ctcaccatca gcgacgaaga gctggagcgg gggctctcca tcctggagga
    11941 gtcggtgatc gaggcattat cggagatccc cgaggaggct ctggataaag atggtgagaa
    12001 gctctggatg gaggttctgg catgatcgtg aggaagctct cggaggcgga gaggaccgac
    12061 cggagggtgg tggcctcaaa ctgggagagc acccgactcc tcctgaaggg ggatgggatg
    12121 ggcttctcct ttcacataac caccatctat ccggggactg agaccctcat ctgttacaaa
    12181 aatcacctcg aatcggtcta ctgcctcgag ggcgaagggg aggtggaggt cctggggggg
    12241 ggcgaggcca gggaggggtt cgacctcggg aggtctgatg ccagaggcga cggaggcggc
    12301 ggcgcaggcg gaagagtctt caagatcgcc cccggcacca tctacgccct ggaccggcac
    12361 gaccgccacc tcctccgggc caaaactccc ctcaggctcg cctgcgtctt caatccgccc
    12421 ctccgcggaa gggaggtcca caacgaggag ggggcctacc ccctggaggc ggaggagggg
    12481 gcggagagga ggtccgggat tcaggggatg tagtctgtcc ggatggggga gaagatctcg
    12541 acggcaacgg aatcttcaag gataagggcg ccatgctcga cgccaccggg tatgcaccag
    12601 ctatccccag gttctgcctc gaactcctct gagccgatgg agaggagcat tctgcccgag
    12661 accatatacc ccgtctgctc ttgggggtgg ctgtgctggg gaaggtggct tccccgagag
    12721 agcctgaact ccgtgaggag gctttccttc ccgtaaacca gggtcttctg gcttatcccc
    12781 gccaggggct gcctataccc ttcatcctcg cctttgtaaa acatgctctc acctctttgg
    12841 tatatttcgg ctccaatcgt cgaagccttc tttctccccc gctgaaaggg gggtcgtcgg
    12901 ccaactctgc cctatcgcca gatctctggc tttacccagc gggcagatgg cggacctcta
    12961 accggtccgg aggacccaaa tagcctcacc ccttattatc gcctcgcctt ggaggtgggg
    13021 cgatgaagat ggtcgatagt accctggaga actgcggctc ggaaggtgat ttgagctgcg
    13081 gaagctgtga gctccatcga gagctgatct gccgggagga tcgaggagag cggaggctct
    13141 tcgtcctgag ccagcttccc ttcttcttcc tctccttctc gggtatgggg gcggtgggag
    13201 ccgccacggg ggtctggtgg cctctcctgg cttattcgat ggcaggagtg gcatttctcg
    13261 gccccgtggg gatggccgcc ctctgccgcc actgccccca ttactccagg ggcggcaccc
    13321 gcctccgctg catcgggcca aaccccttcc ccaagctctt ctccccccgc ctccagccta
    13381 tgagcggctt cgagaggggc gtcgtcatcc tcttcatagc cttaatcctc ctcttcccga
    13441 tcgtcgtcca gggttatgga gcctggcagc tctcggggag ggggccggcg cctctcggga
    13501 tcgcattggc ggggctggga acggccctgt ccgccctcca tctcctccgg accctcaccc
    13561 tccatttctg tccccggtgt gtcaacttct cctgtcccct aaatcgggct cccaaggccc
    13621 tcattgatcg ttatctggag aagaatccgc tagttggggc cgccatggat acggagggcc
    13681 gatagagggg cagagggcca tcgcccccgg aattccggag gaacgaacgg ctaggggacc
    13741 gggtccgttt gaagcaggaa tggaagaaga ggagccccgc ggcatctgag gtgaaggggg
    13801 ctaaagcccg ccctcggcct tccaggaggc gtctttgaat accgtccccg gatacttgac
    13861 ctcggaggtg agctcctcgg tcaccaggcg gtactgcctc tgggttctgt tgccgcgccg
    13921 ctcgaggacg ccgtcgtaga acatcctcga taggtatgta gatacggtgc tgagctttat
    13981 gtcgtcggat acggtctcat accggtccct cagctcgttc gatgagaacc aggagtcagg
    14041 aaactcctcc cggatgaaga tctccagcct gtctctgagg gtacggctgt tctctgattt
    14101 ggggaacggt gcctctgaca tctgatctga cccttcagag aatacgccag ctgcggtcaa
    14161 gaaatcgatg accctctctc ggactatggc cccctcgaat acgtgcgtgg acctcatgcc
    14221 cttatcttcg atctcgatct tgaccttcat ccaacccacc ccctcacgcg agggctgacg
    14281 ggacttccgc tggcatggag aagcccggca gatggatgaa agatcctctg gagtggcagg
    14341 actaaaccgg gccctgtctc ccttgatctg agaacccttc cttcggcttc atcggccgct
    14401 gagtgatgaa gggcgaaccg ggatgggagc cgtctcggaa agctccatct cgatccgccc
    14461 accccccttc attgctatct cccttaccat ttgcagatgt aaatgtgaac ttggagctcc
    14521 cggattcatc cctgagtcgc tccaaagaca gagcaaaacc ccggatcctg cctccccttc
    14581 acttccccta ccaatgaaga accgccgccc ccttcaaagc ggaggtcccc ccttcattgc
    14641 tctgtgatca gacaggatcc gaatttgctt ctgcaatcat cccttgtgtt atattggtat
    14701 ataaaccttt ctgagtgtag ggagatgtag tagccaagtt aaaaccgata aagatggtct
    14761 aatttcaaat ttaagccgaa taaacaggtt taactatgtt cacaaggcct caaataggct
    14821 tcggatatgg acagtcccaa catgagccga tcgatgatct aaaaacaaat taaatcagga
    14881 ggattcctct gcgaccccag gagagatggt gaatatctga aaaagtcctc tggccgccac
    14941 catcgaagat aaattggggt cgggaggggc cttaccatcg caggggatag gccgcctcag
    15001 tcctgccccc cgaggaggtc gccggcccgt cgaccgccag cgcactggcg gcataccccg
    15061 gagcccgggt caagacatcg cgcgcagacg agcctcccac agagctggca ggtgaagagc
    15121 tttcccgccg cgccgcatga gctgcagatc cccaaaagtt ccatggtatc aagacgcctg
    15181 atgcagttcg aagtactcct ccaccagcca gtcgccgatc tctttcatat acttgcgcct
    15241 ccgctcgtcc tccagagcgg cctggtagat ctcggatagg tcttcgtaca tggatgttca
    15301 gagggctggc aagaacccat ttaaatctat cgcgaccccc ggaggcgccc tttttgggga
    15361 ttatctcctc gctggagggg tagcggagcc ccccgggacg gtggtttcag cccaactccc
    15421 tccaccagag gatcagggcc actgaccccg agtcgaaggc cccctggagc cttccggcat
    15481 ggatggggtg gtccccgcag accatcaaaa tcgccctttc cgagatcgcc gcagccatct
    15541 ctccgatgag gccatcgatg gcggatgctg cctcccggat ctcctcgatc cccccacccc
    15601 gatgcgccgc ccggtcgatc ccgacgaagt gggcgacgag gacccttggg tctccttcca
    15661 gggcctcgag ggacctggcc aggatctgcc ggtcgaagtc gggggcactg agggcccggg
    15721 ggactccgag ggcgatctcg acgaagccct ggaaggtccg ggccccctcc tcctccatca
    15781 ccaccgccga tcggaccccc tcggagcccg cccactccag gaggcttctg atctccgatt
    15841 cggaggcctg gctggtgttg ctgatcccgt ggttttgggg taggaggccc gtgaggatcg
    15901 aggcgatggc cggcgtcgtg gaggttgcga ccgcctcggc ctcgaggagg agcccctccc
    15961 tcgccatctt cctcatgttc ggcaggtagg gctccaggga cctgtagagg ccgtagccga
    16021 ggctgtcgac gatggcgagg gcgacgcggt cgcagtccca cccctccacc ccgccgatcg
    16081 gcctcccgtc gatgggaggg gtcttcaccc ccaggacccg ggccatggtg ggggcgacat
    16141 ccacgagctt gatggatctt aacatcttca gcctttccca agaggggggt tacgccgctg
    16201 gagctgccgg atccctcccg gtccaccccc accgatctcc ttccctcgcc agggcccagc
    16261 ctcgcccttc ccgtccccct caccgccggg cgaagtgctt tttgatctcc agccattttg
    16321 gcgtcttccc tttagctcat ctcccgggcg atgagctcct cggggatgag ccggagctgc
    16381 tgccgaaaga tccgccagct ctcagaggaa ggcagcggtc gagattcaaa tcgcccctct
    16441 cctcggatct gtagccattt gtgatcccca tcgatgaaat aggtttggca gcatcgggat
    16501 gttgagaacc tttagatttc attaccctgg ccggatctgc cggattttat aggccctttt
    16561 gcctcagatg gccgaagggt gggagagccg gcctcggagc ccccccaaga aaatgtcccg
    16621 gtatcccggg gtacagggcc cctcccgacc cgagatccca aagggcttat ctggaaggag
    16681 acgagatcag ggcgggggcg gatatattat gatgtcagat gtatatttta ctcccctgag
    16741 ggctgaaggc cccaaggagg gagagggggg gcgggtccgg ggcctatttg aggcggcggg
    16801 ttttgaagct ctgatccggg agggagacct gacggcgata aagctccact tcggggagcc
    16861 gggaaacgag acctacgtga ggccggcctt cgtccgtccg gtcgtcgata aggtgaagga
    16921 ggtcggaggg aggccctttc tcaccgacac caccaccctc tacggcggga gcaggcagga
    16981 cgccgtcggc cacctcctga cggccctggc ccacggcttt gatctttcgt cggcgggagc
    17041 tcccctgatc atcgccgacg gcctctccgg gaagaacttc gtcgaggtgg agataggagg
    17101 aaggcacctc cagggggtga agatcgccgg ggatctggcg gcggcggcct caatgatcgt
    17161 cctctcccac ttcaagggcc actccatggc gggcttcgga ggcgccatca agaacctggg
    17221 gatggggtgc gctcccccct cgggcaaagc cgatcagcac tgggcctcaa agcccgccgt
    17281 ggcagtggag ggatgtacag ggtgcgggag gtgcgcttcc ctctgcccca gatccgccat
    17341 cgtcctggag ggggggaagg cctgggtcga ccccgaggtc tgcaccggct gcggcgcctg
    17401 cctcgcctgt ccggaggggg cgatggatct ggactgggag aggatgccga tcttcatcca
    17461 gcggctcgtc gagtacgccc tgggagccgt ttccggcaag ttgggaaggg tcgggttcat
    17521 caacttcgtc atggatgtca ccccggagtg cgactgcgcg ccctggagcg acccccccat
    17581 cgtccccgac gtggggatcc tcgcctcgaa ggaccccgtg gccgtcgatg cggcgagcct
    17641 cgatctggtc aacgcccagg aggggatcgc gtcctccgcg ttgcagtccc atctccgacg
    17701 gggcgaggat aagttcaggg gcttatggcc gaaggtggac ggaagcctcc agataagata
    17761 cggcgaggag atgggccttg ggtcctcccg ataccggctg atcgagatct gaatcctcgg
    17821 ccaagagttt gaaggcgaag gaggtctcca gggtgcgggg atcgagggtc ggggcggaag
    17881 gataaaacgc cgaggatcct gagggcggcg gtggcaacac ttttgaatct acagggagac
    17941 ctgatacctg ctattgagct atggagagga agaagatggt agatcttttc acggaggttg
    18001 ctggccgggc ggaggcggag gatagaagat acctgatgga gcacgaggtg aaggccgtcc
    18061 tcgaggggct ggggatctcg acgacggggt cgggggtcgc ccgatccgaa gaggaggcgg
    18121 tcgggatggc ggaggctatc ggctaccccg tcgtcctcaa gatcgcctcc ccagaggtgg
    18181 tccacaagta cgacgccggc ggagtgaagc tggacctgaa ggggcccgag gaggtgggga
    18241 gggcctacgc tgagatagtg gaggcgttcc gaggaaagga gatcgtcggg gtctccgtcc
    18301 agaggatggc cccgccgggg accgaggcga tcgtgggggt cgcccgggac cccaccttcg
    18361 gcccgatcct gatgttcggc ctcggcggga tcttcgtcga gatcctccgg gacgtgtccc
    18421 tgcggtcgat ccccatcacc gaggccgacg ccgaggatat gataaagggg ataaaggggc
    18481 ggcccctcct ggagggatac cgaggcaggt ccgcggatat tccggcccta aaggccctcc
    18541 ttctggagat atccgacctg gtggtgagac acccggagat cggagagatg gacttgaacc
    18601 ccgtcatcct ctaccccgag gggtacgccg tggtggacgc gaggatcatc atcggcgagg
    18661 tgcctgagcc atccgccgag gcccctccga aggtccagga cctccacgac ctcttctatc
    18721 cgaggagcat cgccgtcatc ggagcctcgg ggacgagggg aaagctcggc tggaacgtct
    18781 tctgcaacct gatcaaccac gacttccagg gggagctcta ccccatcaac ccccgggccg
    18841 agtcgatcat ggggataaag gcctacccgc ggatcagcgc cgtcaaaaag ccgatagatg
    18901 tcgccgtcat cctcgtccag gcgtcgatga ccccggaggt ggtgaaggag tgctgcgact
    18961 ccggcgtcaa gtacgtcatc gtcgagtcgg cgggcttcgc cgagatgggg gaggcggggc
    19021 ggcggatcga ggaggagatg aaggcgatca ccgatgccta cggcgtccgg ctcctgggcc
    19081 ccaactgctc ggggataata aacacccact gcagcatggt ccagtcgatc gggatcgtcg
    19141 acgccctctc gaaggggaac atcggcctta tctcccaggc cggggtctgc gccgccggga
    19201 tgctctgggg gctgcggcac atcatggact tcgggatcgt ggcgacgatc ggaaataagc
    19261 tcgacataaa cgagacggac atgctggagg cggtgagcca ggacgagaac gtcgaagtca
    19321 tctgcatgta cctggagtcg gtgaaagggg gaagacggtt catcgacgtg gcgaagagga
    19381 tcacgagggt gaagccgatc atcgccctca agtccggccg gaccgaggcg gggaagaggg
    19441 cggtcgcttc tcacaccgcc tccatcgccg gcgacgacct ggtctacagc gccgtcttct
    19501 cccaggccgg ggtgatcagg gcgagggact acgagcacat gttcaacctg gcgagggcgg
    19561 tctccaagca gcccttcccc gagaaggacg gggtcttcat catcacctac gccgggtccc
    19621 tgggggtcat ctccgccgac gccgtcatcg acgaggggat gaggctctcg gagctggagc
    19681 cctccctcaa ggagaggctg ctggccctcc tgccggagta cgtcggcggg acaaaccccg
    19741 tcgactacac cttcagcatg gacgccgaga cggtgaagag gacgatcgag atcggcgtcg
    19801 agagcgagga cgtgggaagc ttcatcgtca tcctccaggc ggagatcctc ggaagctacg
    19861 tcgcgcccct gggcgggatc gactacgggg gcaagccgat catggtctgc gtcgcaggaa
    19921 aggagttcgc catggaggac gtcatcaaga tggagaacgc cgggatcccc gtctaccaga
    19981 cccccgagca gtgcgccgac gccatatcgg tgatgtacag gcactggcag tcttcgagat
    20041 gatggctgga ccttccgggg gtcggagcgg ggcaggagcc gaccggatcc ggggggcaac
    20101 tgccccccca tcgagaacct caggcctgat tccttcggtc ccgatcgatc ggctccatcg
    20161 accggaccga aagatgggat ccgtggatca ggcctggata tccgcttctg ctggtcactt
    20221 cggctttaca ggtcttctac gtcccaccgt catatctgtc cgatgggccg ggtcaataca
    20281 tcttctggac gtcctggggc accgtccact ctccaccctc gaagaccttc acggaggatc
    20341 ggttagctgc atactccatc gttcgagtcc agttcttgct ggactgcaga gtctccgggt
    20401 tgatcatcct tctgtacctg gggctcaagg cggaggccga cccgttcccc gtcgataggc
    20461 tcgctttcgc catctcctcg gcgagccctc tcgccacctt ccccaggacg gcgggggcct
    20521 tcgaggaccc tcccagagac gccggagctc tgaagatctc cgagccgaag taggggtaat
    20581 actggctgta gtcgaccctc gatggcgcgc ccccggagaa aaatatgggg tcggagaagt
    20641 agctcctcca ctcagagagg ctgggcccgg tcccggggtc gaggtagtcg ccgcccgcct
    20701 ctccagccgc cagaagaaag gcagccgcga ctaaaatcga aatacggctt gaaatcatat
    20761 ccagatcact ctcccaggag gggagttatc ccggctccga tttaaattct tttcccggca
    20821 aattacagga tgatctcccc gcaggtcacc gccgcgagat cctcatgacg cagccctcgt
    20881 ccatctcctc gacctccagg tccatcccca ggtcggcgaa gaccttcctc cagtggctct
    20941 cgatgcaggt cttgcagtgc tgctttgcga cgagccccgc ccccaggcgg gcgaaggcgt
    21001 tgatcccttc cttgatggcg cacctctccc gccggatctc cgcccagccg tccccctcct
    21061 ccaccgagac ggcggagccg aagaggttct tgtcgttggt ggcgacggcc actgcgaggt
    21121 ccttcggctc ggatatatcg agccttttga aggtgtccgc cacctggtgg gctcccatat
    21181 cctggaactc acgaagcccc tttttgccga gccgctttgc tacgtagccg ctgcttccgt
    21241 agaagcttcc cagccacatc gccaccaccc gctgctggat ctgctcgacg gggatctcgg
    21301 gaacgtcggt catgacggcg actcagacgg gaggcgatat aacccttccg aggggccagg
    21361 ggggcgacct tctccgccgc cgtcggcccc gggcgtaacc ttaatcgatg tcctatggcc
    21421 tcgatagtaa acataatagt taacaatagt ctatttgtat ataagttaag cggcgatagt
    21481 tattgatatc tttaaaattt atagacaata ttataatata atcaataacg atcttcggga
    21541 atgcttaaat agtttccgag gaaaaattgc caaggtaaaa tcttctggag gatccggaag
    21601 gaaggcggcc gggcagggag ggagaggatg gcaaaaaaat cgaccgaagg atcgagatta
    21661 tttaatcatg gagaatcgga taaagatcat ctttttcatc tcaataaaat tacgatttcc
    21721 accaacaacc tcagatatat ttttaattca ttgatatgta agggcccggg aagggcctcc
    21781 acccttctca tcctggcctt catctccttc tcctccataa cgggtgcctt cgccgccctc
    21841 agtgggagcg tcatcgcccc cgcctccgtc aacatctgcg aatcgacggg cttcgtcgta
    21901 tacataaaca acaccggtac gacccccgag agcgatatct tcctcaacgt caccatcccc
    21961 tccggcttct cttacgacga cggcaccacc gtcatcgcat tccccgactg ctcatcgaat
    22021 cagacccccc agatcaaggg tggaggggcg tatctggagt ggaacctgac ggatataatg
    22081 agcacagaga cgggcatcgt cttgaacgag atatttccta acccgatagg cattgagacg
    22141 agcaacgaga gcttcgagct cta
//
LOCUS       DASM01000078            3633 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_141437, whole genome shotgun sequence.
ACCESSION   DASM01000078 DASM01000000
VERSION     DASM01000078.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_141437"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          706 a         1106 c         1205 g          616 t
ORIGIN      
        1 aggacgagag ggggaacatc accaccttgg gccggggcgg ttcggacttc tcggcgtcgc
       61 tgatcggagc ggccatcggg gcggacgaga tctggttctg gaaggacacc accggcgttc
      121 tgaccaccga cccgaagatc gtctccgagg cgaagaacat ccccgtaata tcgtatagag
      181 aggcgatgga gatgtcctac ttcggggcta aggtcctcca cccccgggcc attgagccgg
      241 cgatcagaaa cggcatcccc gtccgggtca agtgcacctt cgacccccag gacccgggga
      301 ccctgatcgt caaggagggg atggtcaggg aggacgtcat caaggccgtc accctatcga
      361 agaaggtcgc cctcctcacc atatccgggg ccgggatgat cgggacccct gggatagccg
      421 cccgggcctt ctcggccctc gccaacgccg gggtgaacat cgtcatgatc agccaggggt
      481 cgagcgaggc gaacatctcg gtcgtcgtcg acgagcccca ggttgaggcc gcggagatag
      541 ccctcagggc ggagtttccg acgacgatag tccgggaggt ctcccacaac aaggacgtgg
      601 cggtgatcgc cgtcgtcggg gccgggatgg tggggacccc gggggtcgcc ggcagggtct
      661 tcggcgccat gggccgatcg aagatcaacg tcctgatgat cagccagggg tcgagcgagc
      721 acaacatctc cttcgtcgtc agcatcggcg aggcggagag ggcggtccag gagctccacc
      781 gggagttcgg gcttgagagc ggagcgaaga ggtaaggagc gaataggtcg atgaggaaaa
      841 aggcgatgag agaagagacg aagaaggggc gaagagaggt gaagagatga agaagctgac
      901 ctacgccgat tcgggcgtcg acatccgggc cgaggagagg gcgatctccg ccatgaagag
      961 ggtgatgacc ggccggagga aggggatcgg cgcccccatc accgatatcg gccactacgc
     1021 cggcctcatc gacatgggcg agtacgccct ggccatgacc accgacgggg tcggaagcaa
     1081 ggtcctgatc gccagcgaga tgaagaagtg ggacaccgtc gggatcgact gcatggctat
     1141 gaacgtcaac gacctcctgg ccatcggggc tgagcccctc gccttcgtcg actacctggc
     1201 cctggaaaga gtcgacccgg agatgacggc ccaaatagcc gtcggtttgg agcggggggc
     1261 aaagatcgcg aacgtcagca tcgtgggggg cgagaccgcc tccctccccg agatcatccg
     1321 gggcttcgac ctcgcgggaa ccgccatcgg gatgatcaaa aaagaggacg tcatcaccgg
     1381 cgagaggata gaaaagggtg acgtcgtagt ggggcttccg agcagtggcc ttcacagcaa
     1441 cggcttctcc ctggcgcgga agatcgtcga ggagtcgagc tacacctact tcgaccggat
     1501 gcccggctcg gagaagacga tcggcgagct cctcctggag ccgacgagga tctacatgga
     1561 ggctctggcg gtcatccaga aggccaatgt ccacggcatg gcccacatca ccggaagcgg
     1621 cctattgaag ctccaccgaa tctctggctt cggatttgag atagacgatc ccatggagcc
     1681 ccagccgata ttccgcttcc tccaggagga ggggggcgta gccgaggacg agatgtaccg
     1741 gaccttcaac atggggatgg gctgggtctt cgtcctctcc gaggacgacg tggacgcggt
     1801 cctcaaaatg accgacggca aggtcgtcgg cgagatcgtc gacgaggggc tccggctcgg
     1861 agatatggag ctgaagtaga gcggagaacg agagaaagcg ttattggggt acgaggggcc
     1921 gatattccag ataggaagag ggatcagatt tctagatccc tcccactcaa ccctttgccg
     1981 atcttcacca ccccaccact taagtcaatc ttctagcggg atctacgccc ccatctcccg
     2041 cagagcttca accatctagc ctcgacgatc gcctcgacgt ccagctcctc gagggagtcc
     2101 atccgccgat tgaggatctc atcgtaggac tctccctcgc ggccgaagag ccggaccccg
     2161 tttttaaccc cctcatcgac ctggatggcg gtgctcgtag aagtacaaag ttgggacggc
     2221 gatcatcaaa gatttcatcg aggggatatc tggtcatgcc gaggcttttg ccgggggcga
     2281 tgcttctccc agctttgccg acttaaaagg aggtggagga aagatgagaa gctccgaggc
     2341 ggcctacgcg gccctcccgc ccttctccgc cgcctccttg gccatcgagc cgaagagctc
     2401 ctccagcttc cccttgatct ccttaatcgc ctctatgccg ggtccgccga cgtccagggg
     2461 cgacattccg gcgaggtccg cgtccatcag gcttctgtcg aaggggatca tccccaggac
     2521 ggggacgttc atctccgcca gcttctcttt tatcttcgcc tgctcctcgg gggccttgtt
     2581 gatgacggcc atcaccctct cgatccccag gtccttggcg agcctcttga tcctctccag
     2641 ggtctcgacg gagcggagcc ccggctcgac gacggcgatc atgacgtcga cccccctggt
     2701 cgtcccgcgg ccgaggtgct cgatccccgc ctccatgtcc aggatcacca ggtccttctc
     2761 ccgcaggatg acgtgccgga ggagggcccg gaggaaggcg gtctcggggc acatgcagcc
     2821 gcttccgccg gtgtcgatcg tccccatcac cgccagcttc accccgtcgg ggccgacgca
     2881 gccgcacttc tcgatgacgt cgtcgacctt ggggttgagc ttgaacatgc cgaactcggc
     2941 gctcgccctc tcctggacca tctccttgta gtcggtgatg ggccgcgggt tcttggcgac
     3001 ccccagggcg gaggcgaggt tcatgtcggg gtctgcgtct atcgcgatga cgttgtaccc
     3061 gtcccgggcg aagagccggg cgagggttcc ggcgagggtg gtcttcccca cacccccctt
     3121 tccagtgatg gctatcttga tggtaaaacc tccaggactt catctccgat cttcaggtat
     3181 atatggtaag atcggagttt atatcgcttc tggagaagcc tggggacgga ggcggcaagg
     3241 agatggcgga gaagagggga aagacgggga ggacggaaaa gggcgcgagg agaggggggg
     3301 tcgaggtggg gagagagacg gccggatgcc ggggagggga ggagggacgc cggagctgta
     3361 agggccgggg cctcccgccc catccccggc ccgggaagga gcccactccc agacccgccc
     3421 tcggcccctt cgccccggag cttctccgga agaggtacct ccgacgggac ctctccgggg
     3481 acgtcgccga gaccgccgag gagatgttca gccgggtcgc ccggaccgtc gcccgggcag
     3541 aggggaggtt cggcggagat gcctccgcca tggcggcgag gtttcgggac ctcatggctg
     3601 gcgggctctt cctccccaac tccccgaccc tga
//
LOCUS       DASM01000079            6843 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_119886, whole genome shotgun sequence.
ACCESSION   DASM01000079 DASM01000000
VERSION     DASM01000079.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6843)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_119886"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1243 a         2145 c         2006 g         1449 t
ORIGIN      
        1 ggagtggccg tcgatcttcg tcgccaacgg ggacgagccg tatccggcga cggaggagga
       61 ccttctggag atatacgacc tcttcctcac ctacgatctg gatgtgatgc aggagatcat
      121 ggtatacttc ggagccccca gcatcctgga tcggtcgcct gcgggctacc ccgccgagat
      181 caggaagatc agcccccagg agatgtggtt tggctccggc gagagcaagt atcggtccgg
      241 aaaggacctg gcgatcataa agatcgaaac ccccgacgag ctgccgacgg cgaccctggg
      301 cgactcgtgg ggggtggagg tgggagacaa ggtggtggtg atcggctatc ccggggtgac
      361 cacctcctgg cagaacatag agctctcctg ggagaccgac tacgttccta ccgtcacctc
      421 ggggatcatc agcgctgaaa agaggctgcc ggaccggtcg gaggtcttcc agaccgacgc
      481 cgccatctac cacggaaaca gcggcggccc tgccttcaac gctgaagggg aggtgatcgg
      541 gatcgccacc tttggctcgg ggacgaccct cgtatccggc gagtggatcg acgtccaggg
      601 ttacaacttc ctgatcccga taaatgtcgc caagagcttc atatcggagc tgaacatcga
      661 cacggccccg agcgagacga ccaaagcctt cgagaggggc tttgagctct actggaatgg
      721 gagcctttcg gaggcgaggg gggagttcga tgccatcctc aagacccttc atccgaccaa
      781 ccagtacgct actgagtact cgagcatggc ccagagggga tgatcgacgg cctgcgattt
      841 gcccctacta cggaccgccg tcccggcggt gttcttagtc ttcgccctcc tcggcggcct
      901 cttcggagct ctagcgtcgg atctgccggt ggtgggggcg gaggagatcc tggccgagat
      961 ccgggacggg gaggcggtgg agaggttcca ggtcaggatc gtgggggacc tcgacctccg
     1021 gtctctggat gggcgggtcc ggtcccccat caccatcgtc gactgcgaga tcgaaaggcc
     1081 ggtccggatg gagggggcga acttcgatgc ccccgtccgg ttcgaggaga cgaccttcaa
     1141 ggagatggcc ctcttcagcg gcgcccggtt caacgcctcc gccgacttct cctattccac
     1201 cttcgaggat accgtcttct tcgactcggc ccagttcggc ggagaggccc tcttctgcgc
     1261 caccagtttc ggccgcgacg cctacttctc taactccgta ttctccgggg tcgccgactt
     1321 caacttcacc cggttcgccg aggagtacgt cctcttcgac ggcgttttgt tcctcgggga
     1381 cgcccacttc gaggacgccg agttcgggag gtggaccaac ttctacggct ctcgcttctc
     1441 ggaggaggcg gacttcctcc tggctacctt ccccggcaga agcttcatcg ccaacgtcac
     1501 cttcgaaggg gatgctaagt tcgccggcgt cgaattcgac agctttgcgg agtttgaaga
     1561 ctcccgcttc gagggggatt tcttcctata cggctctacg atctcctaca tgaagctgaa
     1621 cgggaccgtc ttcggcgaag gatcccaggt ccacctcaac aacaccgaga tcgaggggtt
     1681 cgtcatcccc tggaggacga tggaggacca cctcgtcttc agggacgagg tctatatggg
     1741 gctcgtcgag aactaccatc ggctcggctg gttccaggac gccgacgact gctactatca
     1801 ttatcgtaag ctttctcagg agagaaaaga gatcggctgg tcgaaggcca ccgactacct
     1861 cgcctggctc tcctgcggct acggcgtccg gcccgactac accttcgcct ggtccctctc
     1921 gattatcctc ctcttcggct ccgtctacta cctcggaggc ggcctcctcc agccggaggg
     1981 gggtgtgtca aagccctcca gcgcgggcgg ggcaaaagag gcctcgccaa tggaggcggc
     2041 cgcagccgca ggctcctccg gcccggagcc tccgggcctc ggggaggcga tctacttcag
     2101 ctccctagtc ttcatcgccc aggcgccggc cgagtaccgg gcccgcggga ggtacaggta
     2161 cctggtgatc ttcgagggga tcctgggatg gctgatgctc gccctcttcc tggtggggct
     2221 aggaaacctg atgataaggt gacgtggagc ttttggtccc ggggcgcatc cttcgcaatt
     2281 aaaatgttgg actgaaaaaa taacttgggt tggcgggccc gatggccgct cacctcaggc
     2341 tgaaggcgtt gatcatccgt ttcgcgtcat cctcgaactg ggagtacctc tccgccagag
     2401 catagtaggt gatgatgtag accctatcct cctttatggt gtaggcgagg aggacctggt
     2461 acgccgtccc ttcaacggtc gccccgtagg cgatgacgta ggccggctcc tcggagatct
     2521 tcatctcccc ctcggccaga acctcgaagt cgtcgtaaga gcttctcagg ttggccagga
     2581 ccgcctcggt gtactcggga aggttagtcc ccccggggag atcctcgatc tggacggtga
     2641 cgtagttgtc ggggtcgatc aggtcctccc ccggagggag gaaccccgcc acgatcccga
     2701 ggtcgttggg gtccgcctct tcggcggtcc aggcggaggg gtaggggacg gcgaagccgt
     2761 actctctgct ctcaaagagg aggaactcat cgaggtcgga gaggccgatc gcccccgagc
     2821 cgtcgacggg atcgtcgaga ccttcggaat cctgggaggg ctcgtagcac gtttggccgc
     2881 tggccagggc ggcggtgaag aatagcaggc cgaggagaag gagactcttc tgcaaaccat
     2941 ttcacctcgc caccctattt cgccggactg ctgataaata ctttttctgg ccgccatcat
     3001 caggacagga atcttccaac ctattcaaag gggaagcgcc ctgtcgacga ggatgagggc
     3061 gtctcgaaat cccaccagcc ccttcagggc ggtggctcca gccatcagat ctggatccag
     3121 ggagatctca gaccagatcc cggtcaacat ggtggtgtgg tcctcttcgg cggagaacct
     3181 ctttatgatc cccttctgct ccgctatagc taccagctgc cgggccagcc tctccccatc
     3241 ggcgatccgc gccgaatctc ctcggatggc gtcggcggcg atcccctcag ccctcttcaa
     3301 agaatcggcg gctaccgcct ctctgacttt gcggggtgtc ttcgggcccg aaacttccat
     3361 ccctgggcag atcacccccc tctcggcgga gatcaccatc gcccgcaggg ccgccacctc
     3421 tgcagcttgg aagagccggt tctcagcgaa cgccctctcc agatcctgta gccagctctt
     3481 caccagatca gagaatctcg ggtccttcga atccaggcgg tcaacgatct ctgggagtcg
     3541 gaggagtatc ctctcgatct cttcaaggct tgcgatctgc atctcatccc gccttcactc
     3601 tggcaccaac agctcccgga cctctccttc agagtcgacg acggaccatt caaaggcctc
     3661 ttcggatatc gagagatgga tcttgaccat gatgctcccc gggtcggact cgcgaccaat
     3721 ctctttcagc tccgaggagg tcatggcgac atcgagccgg gtgttctcat gcaaaaactt
     3781 ggaaaactcc atgaacgccg ccttcttgag atccgttttc agcttctgaa gcctctctgc
     3841 ccccccagcc tccttcttca gcctcggctc caggatcttg gctgcggcgg agattaaatc
     3901 gtcggttatg gtcgttatgc tcaaccgctt ggccgcctcc cccctctccg cggccgagaa
     3961 tagatcggcc ttcaatatca ccggctcctt cttcagagcc aagatcctcc tgtccagctc
     4021 tttctcgatc cgaatctttt catcgtcctt catctcgatc tgggccttct tcgcctggat
     4081 ggccaagacc cgggggaagc tttcgcggat gttggtgagg acccgaccgg tcgcctcctg
     4141 atcctccgca gacttcatat cgcttatcgc caccggaacg tcgagatcga ccttcggcag
     4201 cctgaagtga gggacgggca tattccggag gagcgggtgg ctagcataga tctcggctat
     4261 tctcaccgat tccaggtccg cctggaccct ggcgttggta acctctgata acaaatggcc
     4321 gatgtagtcg cctaaatagg gcataatatc ccccttttac aatatcttca aatccttccg
     4381 caggtctgac cgctcttcga cccttagact ctcgacccta tataaaaatt gagaggtatg
     4441 ggcagctgac gctgccccct cctcttcagc tcactaccgg agccggggcg ctcaccggct
     4501 gccctctcat cgactcttcc aggatgctca ggaccttctc catgcccccg ggcatctcgt
     4561 cttggacggc cttcacgtgg atcgccaggg agtaggtccg gttgacgttc gatccctcgg
     4621 tggtggacct ctggtaagat gtggacacct tcagctttgc cgatccgaag aaccacccgg
     4681 ctttggcctc gagagaggcg tccaccttca ggttggtgtc gacctttctg tactccatgc
     4741 tggtgatctt ggcgttgaaa tcgatggtcg tctcctcgat ccggatgaag gggatcggga
     4801 gcatggtgag gatggggacc tccagcttca tctcctgaat ctgggctggt acggcagggg
     4861 tggcaggggt gtcacccgat gccggaactg ccggctgata tggggagatc tccttggggt
     4921 atttgaaggt cacgtatatc gggtctcccg tgtcgctcgc ctcgggatcg tcgctctcac
     4981 cggccttgaa ccccaccgat tttatgaagt tcactgtgct cagagctgcc tgggcctggg
     5041 cgttcaccac cgcgatcaag ggcccaccta tcatcgactc gaaatcgatg ctcgatagct
     5101 cctgtcctgg gtttaccatg gattatcacc tttttatttt accgctcgtt ccatagctgc
     5161 cggtttccct ggcagcctcg ctgcaaccaa tagttcaagg tggtctcgca atatatttaa
     5221 acacttcgct attatgaaaa atattatttt taaataaaga ttatttttat atctatttaa
     5281 aatttattat ctgaaagatc tagccctccc gccatccccg acctttcggt cctgccccgg
     5341 aggcggccag caaagccttt atcttgcttc agccggtctt ctcccccatg aacctgaagt
     5401 gcgcggtttg catgctgctg atcgcaggtt tagtcctcgc ctccggctgc ctgagcgccg
     5461 aggaggcggg aggcgaagcg gcggcggtgc ccaccgagaa cctccccgag ggcttctccc
     5521 tcatcgccgt cgtcgacgaa tcgactgtgg gcataaacat ggaggatgag atcgccgatt
     5581 tcaggggaga tgaggatatc ggcagagtca acgccaccct gggcaagtac cagtgggggg
     5641 agatgggcaa ggactacgac gctagggtgt tggtgatcga atgcgagacc gaggctgggg
     5701 ccgaggccgc cgtcgcaaac tacctggccc agcccaagta cgagaacccg cccttcgtgg
     5761 gcgtcgacag gttcagcacc gccgtcgtca acggccgcca ggtgacggag atccgggaga
     5821 aggtcggaaa ggagctgaag tacgtctaca tctggaacga cggcagccgg gcggtcctgg
     5881 tggaggggaa cggcaaccgg gccgagagcc tggagcttgc cagcgccacg gggatctgac
     5941 ggcgctgacc ccctcttctt tttccatcgc catattgcct catcatctcc cttcgacgcc
     6001 atcttcctct ctgatcttcg tctcctccct ccatctcctc cccagccctt catccatcgt
     6061 aggcgagggt gaggttgccc tcccgccagg cgaaacgggg tggggatctc cgccggctgc
     6121 cgccttcctc cagggcgtgg actgcaatgg ggagcgcaat ggtggcgtcg acgaagacct
     6181 ggatcatcct cgcctccctc gcgatcttcc cccaggatac cccctcgctg aaggtgcagc
     6241 ccgataggcc gccgaattgg ggggggtcga tggtgtactg gatggcgtag ttgtggccgc
     6301 cccgatacct cccgcagagg tccgccacca ccttcgtctg ctggatgaag ttcttcggga
     6361 cccctccccc gacgaagacg acgccggtct gggtcgactt ctcagtgatc tggctgatct
     6421 cgtcgacgtc ctggatctga tcgacgacta ctcggtgccc ctccctccag gcgaggacca
     6481 tcccgatccc gatggagctg tcggataggg ctggaacgaa gatcgggacc cctgcccggc
     6541 tggcgctccc gaggatcgta tcctccggca tcctccggcc aagcccctcc atcagctccc
     6601 gggaggtgta cgtccggcga gggtccaggg tcttgacgaa ctcggcgacg tacaggtccc
     6661 ccctctggag ccccgcctcg gagacgtaga cgtcgtatat ccggtcgatc ccctcctccc
     6721 tgagggatgc gtcgtcggct ccggggtctc cccggtagtg gacgacgccg agcccctcgc
     6781 agaggtcgtg gaagaggttg gcgccggtgc tgacgacgca gtcgaccatc ctcctggata
     6841 tca
//
LOCUS       DASM01000080            2840 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_273888, whole genome shotgun sequence.
ACCESSION   DASM01000080 DASM01000000
VERSION     DASM01000080.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_273888"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          670 a          763 c          834 g          573 t
ORIGIN      
        1 aacgacaaca ccactctcga gatcgagcag caggcactgc ggagtctcac caagaagctg
       61 atgaaagaga tccagctcgg gatcctgatg gacaattcga tagagatgga aatagaggcg
      121 gcaaaagcca ggaacgaaga cccggagaaa gtcagattta tcgctgaaga gatcctcttt
      181 cctctgcggc agaagattat ggatatggag cagatggtgg tggtaaatca gcagggcgta
      241 atggcgatgg aggttgtgat cagaaataac cgggagctga tccgcggggt ggatagggcc
      301 aaaacggtga ccatatcggc tttgagaacc tcggtgacgg tggccggcgc cctctatcat
      361 cagaaggtcg ccctgaagaa gatcgaaatg ctcaatgata cgacgaacgg catcatcagg
      421 ggcacatctc agatgctgaa ggatcagggg gcggagatcc agaggcgatc aatggcgacc
      481 agcatctccg tcgaggggat gaaggccgcc ttcgccgatg tcatggaggc cctcgaatcc
      541 atcagcacct ataagcagga ggctctgccc ataatgcggg acaccatcaa tcaattcaag
      601 gagctggcgg ccaaaggcga agaagagatc ctgaagctgg agaaggggca ttaaatgtcg
      661 ccgttgggtt gggaagatgt tcctcattca tttaaataaa ggatggaaaa ggagttcgga
      721 gatgatgaat aagatggcca tcatgatcgc cctcatgata tctttgatgg ggtgggcatc
      781 ggtgggggct ggagagatgc gatcgggagg agcgaccacg gaggttcggt ttgagggggg
      841 ctttggagga ccgtggatta gagccgattc gttggacctc ggttcatccc tgccggcaaa
      901 tcaaaaactc tatgggattg gtgaggggtc ggccgcaaag ggagatttgg accccctccc
      961 ctcctggaag gaggggtcag caaagagggc catcctcagt ttcgtctcag atgtcacctc
     1021 cgagggcggc ccgaactacg tggcccccga agataggatc gccaccttcg acaacgacgg
     1081 gactctctgg tgcgagtatc cgaattacgt cccgtatctc ttcatcttcg accgggtctg
     1141 cgatatggca tcatatcacc ccgagtggaa ctccaccgag ccattcagct ccatcctctc
     1201 tggcgagcgg accggggccg gcagcttcag cccgggggag gtgctggaga tctacgtcgc
     1261 caccagctcg aatatgaccg ctgaggagta catggctctc gcaagagact ggctcgatag
     1321 atcctcccat ccggactatg gcctccccta tgcagaatgc gtctataagc cgatgctgga
     1381 gctcctcagc tatctgagat cccaggggtt caaggtctac atagtgacgg gaggagatca
     1441 ggacttcgtc cgggccttct cccaggaggt gtacggcatc cccccggagc aggtgatcgg
     1501 ttcggcggtc aagcaccagt tcatcgaagt cgacggcaac acctcggtgg tgaagcttcc
     1561 tgaggtccag gtcgtcaccg acggccgggt gaaggcggag cagatccagc agatcgtagg
     1621 ccttcgcccc atcctcgcct acggcaactc ggatggggat atccccatgc tcaaattcgc
     1681 cactgggggc gatggtccat ccctgggcct cttgaaccac cacgacgacc ccatccggga
     1741 gtacgcctac gatataggct cctccatcgg acggctggag gaggggctgg agctggcggg
     1801 agagtgggat tgggaggtgg tgagcatgaa ggaagattgg aaatatattt tttaggggca
     1861 ggctgcctcc tcctttttcc gaggtctcca gcacggcaaa aagtttttat cggatgctat
     1921 cgccatacat ttaccatcgg cttgtaaatt agctctcggc ttccgcagga tgtaacaatt
     1981 cccgttggca gaatcgctat gctgaaccca gcatcctcct tatcttcgcc ggagagcaat
     2041 ttgatgggac gaaatataac gaatgggaga aaaaatatga atactggaaa attttccaag
     2101 gcgataattt tattgttgtt cttctggtta tcgtcggcat cggccttcga gcctgaggcg
     2161 gtcggcgcaa accccgacct cgtcgccctc aaagcgggat ggtttccacc caccgtatac
     2221 gacgtgaccg atggggttta cgtcgccgtc ggctacggtc gcgcaaaccc ggtcctgata
     2281 gaggggaccg acgggctgat cgtcatcgat ccgggcgaga gcaccacctc cgcagagatg
     2341 gtgaaggcgg cttacaacga gcatctcgac gacatattct cgagaaagcc cgtcaaggcg
     2401 atcatctaca cccaccacca cgactgccac ataaacggag cttcggtctt cgcgggaaac
     2461 gattctccgg aggtcatcgc ccacgagacc ttcgaggagc acctcttcgg gcctagggag
     2521 gtggtgagcc agatcttccc catcaaagta taccgggccg taaagtatgc tgggatacgc
     2581 ttccagaacg attcgggcta ctttgtaaat ggtggcatct tccccttctc agtccccggc
     2641 ccctcgggat acctcccccc caccctgacc gtcggggaca aacttgagac taacatagcc
     2701 ggggtgaacc tgacgatagt ccacgccccc gccgagacga cggatataaa ctacgtctgg
     2761 ctgccggata agaaggtgat cgttcagatc ggcatcttct acaagtcctt ccccgccatc
     2821 aacaccctcc ggggagcttc
//
LOCUS       DASM01000081            4312 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_215860, whole genome shotgun sequence.
ACCESSION   DASM01000081 DASM01000000
VERSION     DASM01000081.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_215860"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    1924..1935
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          818 a         1293 c         1236 g          953 t
ORIGIN      
        1 aggtggaggg gatctccatc tcgtactccg ggccgccgca gtagatgaaa tcgacggagt
       61 tgcccatgag gtagaagttg atggcgtggc ccatctcgtg ggtcaccgtc ttcttgtcgt
      121 atatcagccc ctcgtagttc aaaaagatca tgcctgggag cttcagggga aagaggctca
      181 tgcagtattc gctggtctgc tttccgttat cggggttcgg gtatacgtcg atcaagttgc
      241 tggtgaccat atctgcgaag atctcgctga agatggggtc catcccggag taagaggtgc
      301 cgatctcggc cagggtctcg gtgtagttgt agatctggtc ggggtcgtcg gtgagctgga
      361 ggaaaagatc gtagggcatc agccggtcca cccccatctt ctccctcttg aactcgtggt
      421 acccataaaa ggcgtctttc ctctccttga atacggagtt catctcctcg atctgttcct
      481 cggagaggta gctatcgaaa atcgtggagg cgtaagcatc ggagtagttc agctcccggc
      541 tgaggaggtc gtccttcttc gccttcagga ggtagagggc agccatcttg tcggactcgt
      601 ccttgaggtg gtagaacctc ttatcgtaac agagcttcct gttctctcgg tccggatcgg
      661 tggccaggag cctgtagtag gattggtagt tgatcgggta ttcagtaccg tcggtgaggg
      721 taacattccc ggcgacggtg acgttgtttg tgatctcctt tatggcctgg gtctcaagct
      781 tcatcagctg atctgcgatc tcggctatga aggcggcgtg ggtctcgttt cgcggcctgt
      841 ggtcagcgta cctgatgtag ccgctctcaa ggtacgtcct atattcttcg agccccggct
      901 cctggccgaa gaggacctcc cactcctctc tcgacagcga cttgagcctg atttccgcga
      961 aggacgtggc tgcataatag tcggcataga aggtctggat ctcggagatg aaggtctcga
     1021 agaagtcgtc gttgacgttt aggctgtttt gggcgtagac gtaggttgag aggaggtcga
     1081 gggagatgga gaggttcctc tcgtcctcca ggtatcccag aaggaccgga ccgttaagct
     1141 cctcgaaggc gagacggtag gtctcgttga tcgtctcggc ctccagcttc agacggtcga
     1201 actccgccct cgcctcttct ctattctcga agagaaatgt gaggttccag gacgtggtga
     1261 tattctccaa attgagatga ggggccgggg ccccctcgac ggcggattgg tcgcctgcgc
     1321 ccgagggcaa caggacgaag accgccgcca aaacgaatgc tcctgccagg atgctaccct
     1381 tcatagttca acaccgtctc cagaattccg ataaacccta attaaaatcc agcaaatcaa
     1441 ctcgccatga tctctaggct acttaaattt tcgtctacaa ttatcccttc tctatccctt
     1501 ctcaataggc cacccgccca gggtcggcgg atcagagcgc ctccagatac cccttgatcc
     1561 gatcgagccc ctcggcgatc ctctcgatgg agctcgcgta ggagaagcgg aggtacccct
     1621 cgccttcggc cccgaagtcg atcccgggcg cgacgccgac ccgggcccgc tccaggatat
     1681 cgaaggcgag gctgtaggag tcggccgata tgtgcctggc gcccgcgaag acgtagaagg
     1741 cgcccgtcgg ctcgactgct accccgaagc cgagcttgcg gagccgtctt atcataaaac
     1801 ggcgcctttc gtcgtaggtc cgcctcatcc ggtcgacgtc gtcgccggcc tcccggaggg
     1861 cggcgatccc cgccctctgg acggcggagt tggcgcagat gaagaggttc tgctgcatct
     1921 tctnnnnnnn nnnnngcgta gagcttggag aagccgttga ggacgacggc gtcgtcggag
     1981 aactccagga tcgagcgctc ccttccagcg tagacgaggc cgtggtagat ctcgtcggat
     2041 atgacccgga cccccagttc ggcgatctcc tccatcctct cgccggataa gaccgtcccc
     2101 gtggggttcg acggcgagtt gatgacgatg gccttcgtct tatcggtgat ccgcgaggct
     2161 accgccccgg ggtcgagctg aaagccgtcc gcctcggcgg ccggcaccgg cacggggacg
     2221 ccgccggaaa acctgatgaa gttcgggtag caggcgtagc cggggtcgga gatgatcgcc
     2281 tcgtccccgc cctccagaag cgccgagaag acgagcatca tcgccgggga ggtccccgag
     2341 cagacgacga tcctctcggg gtcgacggag actccgtacc tttccgcgta gtgctcggct
     2401 atcgcctccc ggagctcagg gtcgccgcag ctgtgggtgt agtgggtgtg gccttccgcg
     2461 agggcggccg cgaccgcctc ccggaccggc cccgggaggt cgaagtccgg ctcgcccacc
     2521 tccaggtgga cgacgtcgat cccctggcgc tccatcgccc gggcgcgctc cagaacgtcc
     2581 atcaccagaa agggggtcat ctcttcggct cgtcgtgaga tcatgttggc gggctcctaa
     2641 ctatgtttca aggataagga caatagacct ttcgcttgcg acttgtagtt caaatattct
     2701 gatcacattc agcaggttca aattcctcga cgacttttaa aggtttgtac gtaagggtgg
     2761 aaggtcccgg cgcacagtct caaaaacacg atgcaggttc accccgaagt aatcgtgtct
     2821 aagcttatct cgcatacctg ccatctcttt ccacgggaca tctgggtact tctggcggat
     2881 ctcgggtgga attttcttgg aagcctcacc tacgatctcc agcgccctga ccacggcaaa
     2941 ggaggtcttt tagtccgctt cgaaggcggc ataatccata tctgcaacaa agctctctgc
     3001 cctctcgatg gcatagagga tgtcctggag gtagtcgagc aactcgcgtt tcccggtcct
     3061 caaaccgtct cgacctcctc caggattcgc cgacctatgg cggtcttgag agcgctcttc
     3121 tccaccaggt ccaccttcgt ccccaagaga tcgctcagat ggttttcgag ggcgatgaac
     3181 tccagaaggg tgaggggctc cccggcatcc tccagatcca ccagaaggtc gatgtcgctc
     3241 tcctcgtccg cctcgccccg aagataggag ccgaagatcc cgagggacgc caccttgtag
     3301 cgcctcgata gctccggcat ctgcgacctg agaatttcgg ctatcacctc ggcgggcttc
     3361 accccccgtt gagatcccga tcgcgtcttc ttctttgacg atatggacaa ttcaacctcc
     3421 gtcctcttca ccccaccttc agctgcgcct cgaccagctc gtcgaggaag gacctcctca
     3481 cctccgcggc gtagtcccag aggtcggggc cgcatgcgtc gcactcccgc ccccgcgcct
     3541 ccatcacccg gaccaggtcg aggagggagg cgtagaggag gggcgccgcc tcccggaggc
     3601 gggcgtcgaa ggcgctcgct gcccccccct ccgtcaggat cagcctgcat ccgcctccgg
     3661 cgccgtgctc gtcccaggcc atgcaaccgg cgcccaggca ggcccgggcc acgaaggggc
     3721 aggcccgcga ctccccgatc tcatctcttc gatccgtcat catctctgcc accagccgag
     3781 ggaatcggct tcgcatctcg atgatctctt cggtctgata ttatatcagc gttctccagg
     3841 atctcaggag gtctccgatc tgatattcaa cttttcttcc atgtttcggg gcactcccca
     3901 gagatatctg gcacgaaatt cgggtttgag gagacataaa taggatgaaa taagcatatt
     3961 tttgggtatt tattaattct tagcaaaatt aattgcatct ctgcgtatcg gcggagttcg
     4021 tcagcagaac aagattttaa ataacatgca gaccgagaaa gttgtttagt tctgtatcgc
     4081 ggatggactt aagatccatc gaccggcccc atccgctaga ccatctcgcg acagatggtt
     4141 cgggccggtc ctggcgaaag atcccgatcc gggattctta ccctcgggtc cttcctgggt
     4201 ccgtccggat gaggaggtct gcttatgatg aaaaaaccct caattgcggc gatccttgcc
     4261 ctccttctgc tggcatcgct gaccttggcg gccgacgccg cctcagccca gg
//
LOCUS       DASM01000082            2645 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_336178, whole genome shotgun sequence.
ACCESSION   DASM01000082 DASM01000000
VERSION     DASM01000082.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_336178"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          514 a          773 c          886 g          472 t
ORIGIN      
        1 aggtgaccgc cccctacaag taccctcgga tcatagagtt cgtggaggcc ctccccaaga
       61 cgatcagcgg caagataagg aggatcgaga tcagggagag ggacgagacg aggggcggat
      121 gaatccccga tcgttgaagg gggcccatta gaggatggca gcgcaagccc gggaggcgca
      181 ggatatggca taccaggtga agctgatatc gaaggaggag aagagggacc tggcggagcg
      241 gtacggtctc caggtgaggt acgaggtgaa gtcggagatc tatggctgct gcatcaagct
      301 gctcactgga tcggaggagg tgagggaccg gtgggaagag agcttctaca acatatccca
      361 gaacataagg tcccacgcga ggctatacgt cgtcagcgac cccgagatgg gggaggaccg
      421 ggtcttctac gacccccagt ccaagaccgc cttcctcgtc aacgtcacct attacggctg
      481 gatcaagtcc ctggccctca gcgccgccgg ggacgtcctg gaggaccagc acgggatcta
      541 ctctgtccac ggagcctgcc tcgacctccg gggccgcggg atctgcctcc tgggcgggtc
      601 gggggcgggg aagacgaccc acacctacgg cctcctgagg aggcccgaga cccgggtcgt
      661 ctccgacgac tggttcttcg cccggatcta cggcgacgac gtcctggcct ttggctcgga
      721 gaagaacttc tacatcaggg cggagctctc ggagatctgg agggagtttg gcgggctcat
      781 cgagaaggca gagttcgatg aggaggggag gggggtcgtc gacctccgtc tcgccatcgg
      841 caagggcagg atcctccccc tcaccaccct ctcggtggtg ataatcctga agcgcgaccc
      901 tgaggatgag gtgataaaga ggaggctctc ccccggtgag gccctctcga tcctggagga
      961 gaacggctac tataaccccc acctcctggt gaaggacgag ttcaaggggg ccctccggag
     1021 cggattcttc cgggagctct tcacgagggt ccccgtcttc atggtcaaca ccgtcaggac
     1081 ccccgaggag agccagaggg cgattggaga tctgatatcc gaagccctcc ggcaggaggg
     1141 cggcctcatg ggaggggggc ggtgatcaga tccggaggat cgggcggagg tctatctatc
     1201 ccccactacg gcctttggcc ggctagggcc tgacgtaggc ccacccttcc gactgcctct
     1261 tcaccagctc ccccgtcccc gtggggacga cctcggcgac ggcgatcacc tcctcctctc
     1321 cgatccccat ggctcggagg gtcctggagc agacggcgaa acggactccc cgggcggcca
     1381 gctcttcgac cctctcgccg aggggggagg ccttgaggag ggcggccacc ccctcggagt
     1441 tggccaggat ctccgcctcc accagatcgt cccccagatc tgccaggagg ttatttacgt
     1501 tgttcagggc aaggctcgcc cgatccttct ctgcaagatg taagatggct cgatacctct
     1561 tcaaggctga ctcctcaccg actcttttct cctgattatg ggagtcatcc ttattctagc
     1621 ctccccctaa accacctcaa gacgagagcc ccgggacgac gaccctttga ggtgatgtat
     1681 gggggcctcg aagatggtct tggagctggt aaggatgaaa aaggaaaaaa agggagggag
     1741 gtataggagg gaggactggc agccgacaac tgccgtgtca tactacggca tttgtcttat
     1801 ttaagggctt cggtccccgg gacgagggct ccagtctctg gcaatgaggc cgatcaagac
     1861 ggatgaaggt cggggcctgc cccatctcct ccgggaccga cagtttgatc tctccatcaa
     1921 ttcactgccc ctccgatgtc cggagatctg gcggtgatat tcgacgttgc tggcaccatg
     1981 ctggagatgt accgggtggc gaaggagatc tcccaggagg ggctcttgga ggggatcgtc
     2041 acaatggagc tggtcatgga ggagggtagg agggccctgg tgatccccca gctccatccc
     2101 gacgaggtcc tgggggcgcc gccccagatg cccctcgcgg tcttcttccg ggggagggag
     2161 aggaatgtaa aggtgagctg ctccaggctt cccgtttccg atgaggaggc tctctcggtc
     2221 ctcctccgct ccggggcgga ggtggcgaac cttcaggaga ccctggccgc ggtctctgcc
     2281 agatgccccg atttctactg cactgcgggg ctgattgtgg acgtcgaggc gggggtggtg
     2341 acccactccg tcagcaccgg agggaggccc tttcctgccc tggaggaggc attggcggag
     2401 ctggagggga tgggggccga tatctacgtc gcctccggcg acagcatgcg gagcctctcg
     2461 gtcctcacca ggtgccgggg gataaagagg gagcggatat accccgcctc caagccccgc
     2521 cgcaaagagg agatcgtcga ggggctgaag gacgattata agctggtcgt catggtggga
     2581 gacggcatca acgatcggta cgccctgaag gcggcagacc tgggggtcct gacggtccag
     2641 cagga
//
LOCUS       DASM01000083            2516 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_68555, whole genome shotgun sequence.
ACCESSION   DASM01000083 DASM01000000
VERSION     DASM01000083.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_68555"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          444 a          786 c          747 g          539 t
ORIGIN      
        1 ggcgctgatg tccatgttgg ccaccaccag ggggatctct ctgccgtcta tgatcacggc
       61 tccgaagggg agggcccccg ccaccagcat agtcgtcgcc accgccacga tgggagccca
      121 gacaaagacg ggcttgtccg ctccccgggg gatgacatcc tcttttgtga agagcttgat
      181 ggcgtcagcg ccgagctgaa ggacgcccca ccggccgccg accctgttcg gcccgtaccg
      241 agcctgaaag tcgcccatga ccttcctctc gagccagacc aagagcatcg ccaccacgtt
      301 gatgacggag gcgaggatgg cagcggcgac tagccctatg atcagagcgt atatcttagg
      361 ctcctgggct gcaaattctg tgatcgcaga taataccaag ttcgttccct cctacctgtc
      421 cacctcgctg gtgcaagcgt ccatgctgcc cgcgatggcg accacgtcgg ctatatatac
      481 cccctccagg agcgggggaa gggcctggaa gtaggcgtag acgggacctc gagccttcac
      541 cttcaccggt ttgtcggacc cgtcgctcac cacgaagaat cccatctctc cccgatggtc
      601 ttcgacgcgg tagtaggcct cgccaggctc gggcttgatc ttcttgggga cgccctcggc
      661 catgatggga ccgttcggga tctggtcgag gcactgctct attatgtagc agctctccag
      721 gatctcgtcg agcctcacct cgacccgagc cttggagtca ccctccttcc tggtgatcac
      781 ctcgaagtcc atatcccgat agaggaggta aggatcttcc cgcctcatgt cgtagggggt
      841 gtccgacgcc ctcaggggcg ggcctgaaac tccgagcctc ctcgcatcct ggggcgagag
      901 ggtcccgatc cccgccatcc tcttctggta gatgctgtcg gaagagaaga gatccttgta
      961 ctccgcggtc ttctcccgga tcatcttgag ggtgtccagg gtcatgtcct tgaagccctc
     1021 aggaaggtcg tttcggaccc cgccgaagcg gacgaagctg tggttgatcc tcgctccggt
     1081 gacgctctct atgcaggtta agacgtcctc tcggtcgatg atcatgtaga ggaagggcga
     1141 gtagacggcg gacgcgatga gggtgagaaa ctcgccggtg cccaggaggt ggctctggat
     1201 cctcgagagc tcctggagga tcatccggat gtactgggcc ctctcgggag cctcgattcc
     1261 gaggagcctc tccacggctc cacagtaggc ctcgttgttg gtgatggcgg ccatgtagca
     1321 gagcctgtcg gtgatcggga tcccttgctg gtaggtccgg ttctccatga tcttctcgat
     1381 ccccttgtgg atgtagccca cgtccaggaa ggccttcttc accctctccc cgtccaccag
     1441 gagatccagg aggaagggtc cggggacgac ggcgtgctgg gggcccatgt ggaggatcat
     1501 ctcagagctg cctttgatct ccatctcctc ccgctcccgg aaggtggggt acttcggcct
     1561 ttccaccggg acggtccccg ccttgatctc agcctcggtg ggtctgaagg aataatcggt
     1621 gtacccctcg gcattgacct tctctatcgc cctgtcagcg gtggtgtagt cggggtagcc
     1681 gaggaagtcc ttcctccagg gccagacccc caccagctcg tcggcgagga ggaggggata
     1741 gagctcaggg tgtccttcga agttgatccc cagcatctcc cagatctccc gctcatacca
     1801 gttggcgttc cagtagagcc ccgtcaggga cttgaccgac gggctctccc tcttggtctt
     1861 aaccttcagg agcgccacca cctgatggga gccgtggctc gccagcatct ccgtcacctc
     1921 gaagaggccc tcgttgatcc tgtccacgcc gacggcgccg gagtagtggt cgaagccgtg
     1981 gttgtccctc aggtacttac atacgtccac gatcctgtcc ggcttgatgg tggcccagag
     2041 ctgccgttcg ggaccgacct tggactcctc gatcactccc ggaaaggccg actgcagggc
     2101 tgccaagaca tcatttgcag ttgtcaacgg tgaactcctc ctctctaata gcccctctta
     2161 tcctcgcgct gcttgatctt ctcctgcagc tccctgaatc cctcgatgaa cttctcgggg
     2221 cgtggcggac agcccgggat gaagacgtcg atggggaaga acttgctggt gtcatttatt
     2281 atgttgtagg agtcgtagaa gggcccgccg gagatggcgc actcgcctat cgccatcacc
     2341 cacttgggct cagggatctc ctcccagagg ttcttgatcg ccggcaggta ctttctggtt
     2401 atgtagccgc tgatgatcat gacgtcgacc tgtctggggg ttgcccgggg gaagatccca
     2461 aaacggtcgc agtcatagtg agggcatccg aagacgagca tctcgacgcc gcagca
//
LOCUS       DASM01000084            8013 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_42367, whole genome shotgun sequence.
ACCESSION   DASM01000084 DASM01000000
VERSION     DASM01000084.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_42367"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1390 a         2460 c         2767 g         1396 t
ORIGIN      
        1 ccaagatcga ggcgaacgtc aagggcggga acgaggcgat cttcctcgac gacgccggga
       61 acgtctccga ggggagcggc gacaacatct tcgtcgtcaa ggacggcgag atctcgaccc
      121 cctacaccgc gaacaacctg aaggggatca ccaggctcgc cgtcatcgag gtcgccctgg
      181 ccagggggta cggggtgaag gaggcaaacc tcggcctctt cgacctctac accgccgacg
      241 agatcttcgt caccgggacg gcggcggagg tcgcccctgt caccaagatc gacgggaggg
      301 tgataagcgg cggaaggccc ggccccgtca caaaggatct gatggagggg ttcaaggaga
      361 tgacgatgac gacgggaacc cccatctgaa cccatccccg acgtcgaaag atatggggcc
      421 ggactacccc gggaggaccc tgacgatgac tttcctccgt cgggggccgt ccagctcgac
      481 gaagaggacc cgttgccaag tccccagggc aagacaccct tcctcgacgg gtatggtccg
      541 gctgtttcct aaaaggacgg cctggaggtg ggcgtgggcg ttctcatcga tcctgtcgtg
      601 gaggtatccg gcccccggag gggcgaggga cataagcctc cggaggaggt cggcgacgag
      661 gccggtctcg gcctcgttga tgacgatccc ggtggtggtg tggagggtgt agacgaggca
      721 gattccctcg gcgacgccgc tctctcccac ggcctcgttt acctggcggg ttatgtctat
      781 cacctgaacc ggctcatccg tctttatctc gagcaaagag gtcaccccat ctgcagagga
      841 gttataatcc ccggcggaat ttaagccgaa cgggcgggga tcgccgcgta tcccttatcc
      901 aaaacctctt agtgagaggg gggccctttc tcagctgacc cacttcctcg aaaaaaatta
      961 tggcctggcc cctcctaaaa ggccggcccc tcggagaacc tcaaggtctt ctccacctcg
     1021 aagaccccgg agaaggcctc gtgccccttc atgtccttat agaagatctt gtgccaccgg
     1081 tagtcgttct gccaggtgcc gttgaagctt gtggcgatgt ccatccccac caggtgggcg
     1141 gggctcctcc ccctcaggtc cgcggcctcc gcggggtcgg aggcccgaga gccggggtcg
     1201 gtggaggcga agtaggtctt ctggacccgc tccatctccg tcacctcgaa ggcctccttt
     1261 acctcggcgc cgatgccgcc gtagaaggcc ttggagtgga tgaacttcgc ccccaccagg
     1321 ggggtcttgc cggagtagct catctggacc tcctccagga ggttgagggg cacggaggtg
     1381 ccgttcagag tcccccagac gtctccggcc cggttggaga cggcgctctt ctggtccatc
     1441 tcgagccggc cgtctccgta gacgtaggtg tagtactcca gccccatcct cctgtcgacg
     1501 acggaggtgc ccacctcgaa gtagccggtt cccgatgccc tgaccctgtt gtagaagtcc
     1561 tcgctctggg caggctcggg gatcgccgga tttccggcag cccctcccat cgccaggacg
     1621 gcgatgagga ggatcgctcc aaaagtcgcc cccttcgtcc ctctcgcccc acaatagctc
     1681 cttgctccac tttatcgctt attaattgaa atatgtgtcg tttgaagggc cggtccggat
     1741 ggcttaaatc tctaaaaccc agatggtgag gggagaaccc agaaatcctg gcggtggaga
     1801 acgaagggaa tagaacggca gagggaggat ctgaggtggt ggcttgaaga tcgaggtctg
     1861 gacctggggg acgggagggc ccttcggccg ggagctgggg gtcggggcga atcgacggcg
     1921 aagagggcat acggcgacct cgatcgtcat cccggctggc gaggctggag gcgaccccgg
     1981 ggcccacatc ctcatcgacg ctggggcccc cgtcgtcgag cggatgatcg aggcgggggt
     2041 caccgcccca gacctcctct tcctcaccca cccccacttc gaccacgtat cagacctcga
     2101 caggcttgcc aacagcagga tgaggggcct gatggtccgg tcgggggtga gggattttga
     2161 gagggcggcg aggctcttcg gccccctgcc ggtgatcggg accgagggct gtctccgcca
     2221 cccggaggac ggggtcatgg ggaggttcgg ctacctgggc ggcctcgtct cctggaggcc
     2281 cctatcggat tacgacgtct ggcagagggt ccggaggccc gacggcgccc tccttccccc
     2341 ggagtcccgg ggagaggcgt tgatgctggc gggggccagc cggaggagcg gggaggcggt
     2401 cgaaggggca ccgtcggaga ggttcggtca tccctatccc gggtcgctct ttcccttcga
     2461 gatcaaggcc ctcccggtcc gccactcaaa acatgccccg ggctcctgcc tcttcgtatt
     2521 caggttgggc ttcggtccag tcggcagtcc tggcggagac ggcccgaggg taaggagcgt
     2581 cgtcatctcc ggggacttca aaacgatgga ggagggggtc ctagagagcc ccgacctccg
     2641 ggaccccgcc tgcctcgtcc tcgacgcgaa caccatcaag gcccccggag attatcatgc
     2701 gagcctggag gaagaaaggg ccctcatcga ccgatggacc tcgggggagg aggaggtcct
     2761 ggtcctcttt aaccacctct ccggcttcgg ggactatctg gaggggttct acgaccacgt
     2821 ccctgacgat gaggagtgga gggcggcggc gaggtttccc accaagccga aggtgacggt
     2881 caggatcgcc gaggacggga agtgctatcg tatatgatct ccggggatag aagcgctacg
     2941 gagcctttaa gaagatggcc gactgtggcc aataccacga gatccgtcag ggggatgtca
     3001 ccatcttgag aactgagccg tttctggtat tactgacggt cctcctcctc gcccaggcgg
     3061 cgagctcgaa agaggaggcc gtcgacctcg gaccccaccg ggtcaccttc agcctcccct
     3121 cctcggtcga atgcgccctc acggtggcgg agcccgcaga ggggaggacg cccaccggga
     3181 tagattacat cgagaggtcg atagacctgg actgcggcgg gggtcgggct tcgatcagtt
     3241 tgagggcgca tcagagcccc gtcccggcgg gggacggggt cgtcagggcc ctcagcaaaa
     3301 agaccccccg ggggatcggg ttcgtggggg tggagaccga ggcgaggctc gtcgaccggc
     3361 acaccggata tctgacgacc ttctctttgg cgaggggggg tgaggtctat caggccgcct
     3421 actggctcga tcggcacctg gccccagggg acttcatggg atcgacctat tgcgtcatct
     3481 cctccagcct tccctggacg gcgaccaagg agctcctcga ctcgatccac ctcgaggtga
     3541 aggaggaggc ggtcgggacc tcgacggtgg agacggtggt ggtcggtccc tatcgggtct
     3601 acttcgacct cgggggtctg aattacaccg tctttacgga gggtcccgat gcaggcgatc
     3661 ctgagcctgg ggcggagagg tacagggcca tcctcgcaag tgggcagcgg agggcgacga
     3721 tcgagatcga cggctacgaa gactacagga tggtgaacct ggagacggag cggctcctcg
     3781 ccgaggccca cctccaggcg aaggggtaca ccaggaacaa cgggtcggag tggacggtcg
     3841 gtggagaggc cggcatcctc agcgtggggg agaacgagga ccaccagatc ctcttcgtcg
     3901 ccatcttctg gccaggccag gtcgaggccg gggagggggt gctcctggcg aggaccaggt
     3961 gcgaggtcga gtcgagcttc ttgtggaacg cgacggagat cttcctcgac tcgatcaggg
     4021 tggagaggat cgaggaggtt tgatctttct cggacctcgg gatcggggag gggccggctt
     4081 cctggcggtt tttctcctcc ttttcctctc ttctgccgcg gccttccagg ctgaaccctc
     4141 gcccccggcc gtcgccatcg tcagggtcga gactaacccc cccggctcgg acgggggccg
     4201 ggagtgggcg gagctcttca acgacggcgc gttagacgcc gaggttggtg ggtggaggat
     4261 tttgacccgg acgggggagg gggtgacgat ccccccgggg acgatcgtcc cttcccgggc
     4321 ccgcctgagg gtagccttcg atgggggcga gggcccctcc ctatccgatg agggggagtc
     4381 gatctccctc ctcaatgagt ccgccagggc tgtcgacgaa accccacccc tgaacgattc
     4441 ggagaactcc cacggcgtat ggacgcggct ctcggaggag ggggatgctc tagttggagg
     4501 ctggcggttt gagcccctct tttcgcggat cgacgaccac gccctccggg cccccccgga
     4561 ggcggaggtc tccctcgaaa atctcgccct atacctgggg gccccggcta aaaacgacga
     4621 agagcggacc cgggcgatct accggtggat caccgacagg atcgaatacg acgtcgaggc
     4681 cgccatgaag ggaggcggca ggggccggac accccaggag gttttagagg atcgtagagg
     4741 ggtctgcagc gagtacagcg ccctcttcga gaggctctgc cagctatccg gcctggaggc
     4801 ggaggtggtc cgggggtatg ggaagggtta cggctacgcc gtcggctcgg ggataccccg
     4861 catcagcaac cacgcctgga acgccgtcag gatcgacggg acctggcggc tcctcgactc
     4921 cacctggggg gcgggccacc tcgatcccgg agaggggttc gtcgagagct tcgaggagtt
     4981 ctacttcctc accccccctg aggatctggt ctggacccac ctccccgatg atcccgcctg
     5041 gcagcttctg gagacgccga tatcgaggga ggagttcgag gccctcccct acgcgaagcc
     5101 cgccctcttc agggacgata tccagataga tagtcccccc ggcggaacga tcgaggtcga
     5161 ggagatcccg accgtcactt tgtcggcccc ccctggggtc tctatcatcg ccgggcttcg
     5221 ggacggttcg gggacggagc tacccggccg tttcgtccag gtccagaggt ccggggaaaa
     5281 gatcctggtg agggctgccg cccccggccc cggagactac accctccgga tttactcgag
     5341 gaggggcggc ggcgagggtg gggatcagaa agtctacgac tgggcccttg actatcgtat
     5401 cgaggccggg ccggatgctc ccgttgagac ggggtttccc gtcgtctggg atctcttctg
     5461 ggatctgggc ctcgacttca agagccatcc ccaggggctg atcgaggcgg gctcggagtt
     5521 ctcggtaatc atctcggccc ccggggacgt ccgcctcctg gcgaggctcc tggacgggga
     5581 gggccgggag ctccccgagg agaggacgat cgcccagagg gtggtggaag aggagggcgg
     5641 atacggggtg acggcatcct tcccgaaggc ggggaactac acccttcgaa tctacgcgaa
     5701 gcggggcggc gaagccggcg gcgagtacgc ctccgtcgcc gagtactcgg tggtggcctc
     5761 ctccgggagg gtggaggccg tctatcccag gaccaccgga tccttcgccg aagtcggagc
     5821 tcttctcatc cgccccctgg aggggaggct cgccgccggg gaggcggtcg atttcgaggt
     5881 catcgtcccc ggcgccgagg atgtggcggt gatctgcggg ggggaggcga cccgcctcgc
     5941 ccgggacggg gagatcttcc ggggatcggc ctctccggcg ggggaggtcc aggtggcggc
     6001 ccggttcgga ggcgggggag cctatcaggt cctcctcgtc tacgccgtcg tctgaggcgg
     6061 ggttcggtac aggtcttcat ccggaggatg gatcaaagga ggatgcgaga ggtcggagga
     6121 gaaggggtgc tgagggatga gaagagaggt tggggatgat gctggaggtt cggaggtcgg
     6181 gagggagcgg gatcggggcg gcggtcccgg agccggcagg ttgaaggagc cggaggctga
     6241 aggtagggtg gtggcaacgg actccatcgc ctctgacccc gtggcgcggg tcgccctcct
     6301 ctccctcctc gtgaacctct ccctggtcgg gatcaagctc ctcctctcgg agatatccgg
     6361 aagcctcgcc ctccgggccg acgccgtcca ctccgtcgtc gacgtcttcg gatcgacggc
     6421 cctgatcctc ggggtcgtca tctccgggag gaggagcaag agcttcccct acggcctcta
     6481 caaggtggag aacctcgtcg ccgtcgtcat ctccctcctc ctcttcctca ccgcctacga
     6541 gatcggccgg gaggcgatga tggggaccgc cgcggaggca gcccccggcg gctgggtcct
     6601 cctggcggtg gcggccctcg tccccgtacc cctcctcttc gggagctacg aggtgaaggt
     6661 cggaaaaaag cacagctctc cgagcctcgt cgccgacggc cgccagttcc gggccgacgt
     6721 tctcaccgag ttcgtcgtct tcgccgccct ggcggggcag ctagtaggcc tgcctctcga
     6781 tagagtggcc gcagcgatca tcgccgtcct catcatcagg gccggctggg agatcctcgt
     6841 ctcggggatg agggtccttc tggacgcctc catcgactcg gcgaccttga ggaggatcga
     6901 ggcgaccatc gaggcccacc ccgaggtctc aatcgtggag gaggtcctgg ggagaaactc
     6961 ggggcgctat cttttcgtcg aggcgaaggt ggccttccgg gtcaccgacc tcgcccgggc
     7021 ccatcaggcc agccagcgga tcgagcagga tattagggag gcggtcccca acgtcgaccg
     7081 ggtcctgatc cactacgagc cgaagccaaa ggaccacatc cgttacgccg tcgccctgga
     7141 ggacctgggt ggaaaggtga gccgccactt tggggagtcc ccctacttcg ccctcgtcga
     7201 cttcgacctc aaagggcggc gggtggaagg gcagacgatc atatcaaacc cctaccgaga
     7261 gctggagaag ggaaaggggc tgaaggtggc agccctcctc ctcaccaaga agcccgacgt
     7321 cgtcgtcgcc ggcgagagcc tggcggggaa gggtcccggc tatgccctcg ccgaggcggg
     7381 ggttgagacg gtgatgacgg aggcggctga cctgcaagag gtggtggcgg agatgggcgg
     7441 agcgtgagag tcggtaactg atgggtcaaa atggagggtc aaaaccggtg gcgtctccgg
     7501 caaaatggcc ttggcggcgt gcgataaatg gagaagaggc ggggattgaa gcgcccccgc
     7561 ccccatcatc cttctaaaag ttccccgcta cgatgaccga ccacgtcctc cgccagccgc
     7621 tatttgcctt caagagcgcg aggtttccgc cgccgtccgt gctgtaaaac tcgccctgtc
     7681 ctgcggccgg atcgtagaag aggaggtcgg tgaatccgtc tccgttgaac tctccgggga
     7741 cgatcatcga ccaggtcctc cgccatccgc tgctggcctt caacaggcgg atgttcccgc
     7801 tcccgtcggt ggcgtagaac tccccctggc cggcggcggg gtcgtagaat aggaggtcgg
     7861 tgaatccgtc tccgttgaac tctccgggga cgatcgatga ccaggtcctc cgccatccgc
     7921 tgtgagattg gaggagtctg atgttcccgc tcccatcgga ggtgtagaac tcgccctggc
     7981 cggcggcagg gtcgtagaat aggaggtcgg tga
//
LOCUS       DASM01000085            3554 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_10034, whole genome shotgun sequence.
ACCESSION   DASM01000085 DASM01000000
VERSION     DASM01000085.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_10034"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    3243..3344
                     /estimated_length=102
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          629 a         1161 c         1093 g          569 t
ORIGIN      
        1 gaggtggcgg acgtcgccgg ggtgacggag gtcaccatcc ggaaccgcta caaggagctg
       61 gccgaggagc tggggatcga gatcatcctc tgagctcctc catggacctg gcgaggaccc
      121 ccgtagtctc cgccagggcc agatctattt tccaggcctt gtctagcctc tcctttgcca
      181 tcctcaggga gtagggctcg atattccagt ttactccggg gtatccgacg tccatcatga
      241 ccagcccctc cgccggggcg ggggcgaccc cccgattagt ccggggatcg aggagggctc
      301 cgacccaggc caggtcccgg tctccggacc ccaccagctc cagcaccttc gccatcttcc
      361 tcaccatgtt ccagaggaag ccgtcggctc tgacgtcgat gacgacgatc cccctctcct
      421 ccgtcagatc cagcttcatc aggttccgga tcgatggccc cctcttctcc gaagagaggt
      481 ttctgaagtc gtggacgccg aggagctctc ctgccgcctc ccgcatcgcc tcgaggtcga
      541 ggccgccgcc ggggaggtgg taccggtact cccgccagag ggcggcgtgg cgggcgctga
      601 agttctcggg gacgggggcc caggcccagg cccagacgtc ggtggggagc cggctgttta
      661 cgacccgggg gacggcgaga tcggccttgg caggtcggac gtagaagtcg atcacctggc
      721 ctagggcaga taccccccgg tcggtcctcc cggcgtagca gaagctcccg tcgtttatcc
      781 ccagggaccg gagggcgcag cctaccacag cctggacggt gggggcggca ggctggagct
      841 ggaagccgtg gtagccccgg ccgaggtagc cgagcttgaa ggcgactttc atgttaacgg
      901 atcatcccag agagcttcgg tggtcgaggc tgtagggatc ggacctgcta ccggcttcgg
      961 gtcagatctc cctcgggtcg gcgatctccc cggtgatcgc cccggccgcc gccgtcgccg
     1021 gggagcagag gtagacccgg gccttggggc tcccctggcg gccctggaag ttccggttcg
     1081 aggtggagag ggacgcctcc ccgtccccga ggaggccgaa gcttccgccc atgcaggggc
     1141 cgcagcaggg ggattcgact atggcacccg cctccatgaa cttctcgacg agccccgccc
     1201 ggagggcctt caagtactca gtcctgctgg cggggatcac caccatccgc accccccggg
     1261 ccacgggctc gcctctcatc acctcggcgg ccagggctag gtcctcgaac ctgccgttgg
     1321 tgcaggagcc gaggaagacc tggtcgatct tcgtcccggc gaggtccccg gcggggacga
     1381 cgtggtcgac gttgtggggg acggcgacct ggggcggaag gtcggagacc tcccagcccc
     1441 gctcggtgaa ggaggcgtcc tcatcgccgt ggaggacctt ctcgccgccg aagtccggca
     1501 cccgttctcg gatgtaaacc agagtcgtct cgtcggggtc gacgaggcca gccttccccc
     1561 ccatctcgat cgccatgttc gtcatcgtca tcctctcggg gacggacatc ctcaataccg
     1621 cagagccggc gaactcgcag gcctgatagg tggcgccgtc ggcgccgatg tcgccgatca
     1681 tcctcaggat gacgtccttg gaggtgacga ggcggggaag cgtcccctcg gccctgagcc
     1741 gcaaggtctc ggggaccatg aaccagagct ttcccgtggc gaagaccgag gccatgtcgg
     1801 tgctgcctat cccggtggag aaggccccca gggcgccgta ggcgcaggtg tgagagtcgg
     1861 agccgacgat gaggtccccg gggaggacgt gccccttctc cggcatcacc tggtggcaga
     1921 ccccctcgaa gacgtcgtag ttgagaatcc cctgcgaggc cgcgaagctc cggagcatga
     1981 tgtggttctc ggcggcggcg aggctatctg ccgggacctg atggtcgaag aggataacta
     2041 tcttctcggg gtcccagacg tgcgcgtcct cgcctgcaat ctcgcgaaac cccttcaccg
     2101 ccagggggcc ggtgatgtcg tggaccatgg ccctgtctat atcggccatc acgaactccc
     2161 cggccctcac caccttcttc gaggctcgag agaagatctt ctcagagagg gttagacctg
     2221 ccatggccag ggagatcgcc gccaaaatag ataaagcttg acccgacccc cccggggttc
     2281 gatgatgaga tggcgggatg ggaggacgga tcgataacgg actttattgt cgaaatttag
     2341 agagagaaag agtccccaaa acccttaaat aacgccgcca tcattacccc aatgcacatt
     2401 agagtacatc aaaactcatc gtttctggag gatacacctt tgcaaactaa aattctgctg
     2461 gatgaagaag agatgcccaa gcggtggtac aacgtcgccg cagacctcat cacgcccctg
     2521 gacccgcccc tccaccccca gaccggagag cccctcaagc ccgaagacct ggaggcgatc
     2581 ttcccgaagg ggctgatccg gcaggagatg tccaccgagc ggtggatcga gatccccgag
     2641 gaggtccggg agatcctcaa gctctggagg cctacccccc tcttccgggc ccaccggctc
     2701 gaagagcacc tcaagacccc ggcgaggatc tactacaagt acgagggggt cagccccgcc
     2761 gggagccaca agccgaatac cgccgtcgcc caggcctact acaacatgaa ggagggggtc
     2821 gagaggatcg ccactgagac cggcgccggc cagtgggggt cggccctctc catggcgacg
     2881 tgcctcttcg atatccagtg ccgggtctac atggtccggg cctccttcga ccagaagccg
     2941 taccggaagt ccatcatgca ggtctggggc gccgagtgcg tcgccagccc cagcgagctg
     3001 accaacgccg ggagggcgat cttgaaggag caccccgaca ccggcggaag cctggggatc
     3061 gccatctccg aggcggtcga ggacgccgcg acccacgaca acaccaacta cgccctggga
     3121 tccgtcttaa accacgtcct cctccaccag acggtggagg gccaggagct gaaggagcag
     3181 cttgcgatcg tcgacgacta ccccgacgta atatacggct gcgtcggcgg cggctcgaac
     3241 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnacgact tcatcccgcc
     3361 ctcgatccac gccggcgggc tccgttacca cggcgacgcc cccctcctct gcaacctggt
     3421 ccacgaccgg gtgatcgagg cgatgtcctt ccaccagaac gaggtcttcc aggcggccgt
     3481 caccttcgcc aggaccgagg ggatcctccc cgcccccgag tcctcccatg ccataaagcc
     3541 cgccatcgac gacg
//
LOCUS       DASM01000086            9732 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_83192, whole genome shotgun sequence.
ACCESSION   DASM01000086 DASM01000000
VERSION     DASM01000086.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_83192"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    8192..8205
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9046..9078
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2127 a         2752 c         2751 g         2055 t
ORIGIN      
        1 cccggcgatg acgtggcttg cctcaagaga ccgcgagaag caggcggagc cggtgctcac
       61 cgcaaacccc ctcatgtcca ggtggagggt gatcgactcc ccctccacaa gatggaaggt
      121 gacgttggcg ttctggggcg tcctcttctc cctgtgaccg ttcagggtcg tttgggggat
      181 ctcggcgagg tcccggtcga ggattcggtc tcgcatggac ctcaaacgct cgttctcttc
      241 gggggtgacg agttctgccg ccgcggcaaa tccgacggct ccgggaatgt tctcgatccc
      301 ggctcgtcgg tcgaactcct ggtagccgcc gtccatccac ttggcgatcg gcgtcttctc
      361 ccggatgtag agcccgccga cccccctcgg tccgtggatc gtgtgggcgg agatggtgat
      421 caggtctgca gggacccgat cggcatcgag gggaagccgc ctgaaagtgt gggtcgcgtc
      481 ggtgtggaag aggacgtgtt tgtccatgca gatctgaccg atcgcctcgg cgtcctggac
      541 cgtcccgatc tcctgatttg cgtgctggat cgagacgagg atcgtatcct ttcgaatcga
      601 ctctgcgagc cggtcggcgt cgacgaggcc gtcgccgtcg acctccaggt acgtcacctc
      661 gaagccgtcc cgctccaggg cccgggcgct gtggaggacg gggaagtcct cgatcttcgt
      721 cgttatgatg tgacgcccct tctttttgcc gagggcgagg gcgacgccct tgatggccat
      781 attgctcgac tcggtggacc cggaggtgaa taccacctcc tcgggaccgg cccggagcat
      841 cccggcgagc ttctccctcg ccgcggtcag gccctctttc gcctcgatcc ccatggagta
      901 gccgaactcc gaggtcgcca cggcgtagac ctcgaagaag tagggcttca tcgcctccag
      961 gaccctctcg tcgagcctgg tggtggaggc gttgtcaaga taaacggaag tctcgaacat
     1021 cttcttctga tccctcctga tctctcctca ctccaaaatc aaaaagcaaa aaaccgacct
     1081 ctcagatgaa aaggttgatc gccgaccctc tcgcctcctg gacgtaggtc cccacggccc
     1141 cgtactcgtc gatcaggtct gccctgaggt cctcctttct gatccccatc acctccatcg
     1201 tcatctcgca ggcgataatc ttgccgtcca gatccttgaa gtcggcgagg agcttttcaa
     1261 gcgatgccac ctttgccgcc ttcatccgac gcaacaccat ccaccggccc aggcccagca
     1321 tgttcatctt cgagaggggg cccttctcca gaccgccctt cttcagccgc aaaaggcccc
     1381 agaaggtgaa gaagagggac gcctccatcc ccatggcgag ggccccgttg gcgatgatga
     1441 gggcgctgta gatcttgtcg aggtcgccgc tgtgaacgat tatcgtgacc ttatcccttt
     1501 cttccatccc tatcccttcg gattcttgga gccaaacgta atcgtccgtc atctccttat
     1561 cctgatccgc cattctcccc cctcctcgcc ctcgacctcc agaagctcaa ggcccaaggc
     1621 cttcaccgcc attggtatct cctttttgga ggcgggatgg ttccccgtca cctccaccac
     1681 atcgcccgga gccgccttct tgagggcctt tctcgtctcc accaggggca cagggcagtt
     1741 ctggcccctg cagtccacct tgatctccgc caatcgcacc accgcctcgt ctcgcaatcg
     1801 ccatcacccc cggcggcgag agaccgtcgg ggggcataag tcctattatt tatggtgctt
     1861 ttctttctga aagagttttc cccgccgagc ctaataattc gggcgggatg gtgcgatggg
     1921 gcggtatctt tcgatgaagg attggagtct cttcataacc cgtcaaatta ctaaacagac
     1981 tatagcggat caaaaataag ttaatctaaa gcgttaattt gtccccgttg catcagaaac
     2041 atctatctag aatcgaccta gagaagatcg agatagtgtt cggagggagg ggcaaaattg
     2101 gtcaaagctg aggacatcct ggccgatata ctggcgggta ggtcggtaaa atactacgaa
     2161 gagataatcg aaggcgactt agacctgagc aagctagccc tacccagaga tgacaacggt
     2221 cgcgtaatcg tcaaagtccc gatcgagata atgacgaatt ccgaaattcg aggtgaagtg
     2281 aaaatccgga aagcagtctt tctgaagtcg gttcgcttca atggagctgc gttcataggc
     2341 gatgttaact ttcatgattc gatattcagc agtcaagcca actttacgaa tgctcagttc
     2401 agatgcaatg cagatttcag caatgtgaag ttcggtctcg atgcttactt cagaaaaagc
     2461 aaagttcatc ggagatgctg actttcacgg ggctcagttc ggtggcgatg cacacttcgg
     2521 ggaagctaaa ttcgaaggca atgcgagatt tagctgggct gaattcagag gcgacgccta
     2581 tttcttgatt gcccaattta ataaaactct gggcttaaac aaattaaatt tcaaaaaatt
     2641 gtatataacg tgggattcga ttaaagatag attgtactat tatgataaaa atcacatgga
     2701 cggcccttat aaaaaccata cagacggtcc tacataccta gaattaataa aaaattttaa
     2761 cattattgag cagttcgatg acgctgatga ctgttactat agataccgta aggagagcca
     2821 ggatcacaag agattgtata gtggtgaacg tggctgggat ttgtcaaagc tttcggatat
     2881 tgcttcctgg gtatcttgtg ggtatggtgt tcgtcctatt cacacagtta tctgcatgct
     2941 ttgtatcgtc gttatcggat gggtcttatt tataggcagc tcagaatcgc tttttgagag
     3001 cttctatttc agcgtgatga cgtttaccgg cggggatcca gataacctaa cgcctggaag
     3061 atggcttaga actgtcgtca tgatagaggc gattctcggc tatctattca tggcattgtt
     3121 cgtggtcgtt ctggggcgca agttcatccg atgatacgac accccgcatc cccagctaca
     3181 gctgcctcac caccgtcccg ctgatggcga gaaagaagag gatcgagaag agcgccccga
     3241 gaaccctctc gaagatgaag gcccccctta tcagagatcg ggaccgtccc cttatcttcg
     3301 ggaggggcgg cggatcgatg aagagtctcg tcccggagag gaagaccgtg gcgctgaagg
     3361 cgagtacttc aaggatgctg tcgtgctcga aatctgacag agtgtactcg tctagcgcct
     3421 cgtttggtct gcccagaccg attgaccgcc agaatatccc gaatactaat atgatcatca
     3481 gcgagaaacc tagcgttctg aggggctttg tgccgtatcc atagctcacc tgaaggaaat
     3541 aatcaatagg gtttttaaat aaaatattct ccaaagttag acgagccatg cttcggcgat
     3601 cttttctata ctcaaagtag caattgtcat aatcttcgag gtagtttaaa tctctgaaat
     3661 tttttagtaa tgataaataa gctatatcat catagactat ccctttctca agactatgcc
     3721 atcggatgaa tagcttatcg tacctagtcg ctattaatga aagctcgccc ctaaaaatag
     3781 cattctcgaa agtagcatct tttataaacc agacacctgc aaagtcagcc gttccgttga
     3841 actgagcgta attaaagcca gcgattgcac cgaactcagc atctttgaag tgggcattcc
     3901 cgccagtaaa ttctacaata aaccctgttt ccaagaagcc gtaaatccca ccgaactcgg
     3961 ttttataaaa gtaggcatta ccatcgaact tagcttcctc aaaataggca tccctcccaa
     4021 acgttgcttc agagaaggtg gtatccccat agaacttcgc tctgctgaag aaggcataac
     4081 tggcgaactc agctccccag aagcttgtat attcgccgct gaaattggca tcaatgaaac
     4141 tggcatcccc gcagaactct actgtaccaa agtgggtact cccaccgaac tcgacttctc
     4201 gaaagcttgc ttctccgctg aacttggctt ccgagaagct ggcaatactg gcgaacttgg
     4261 cctcctggaa gcttgtatcc acgccactga aattggcttc agagaagtcg gcaccccatc
     4321 cgaacttagc cctctgaaag tttgtataat tgccgctaaa atttactcgc gagaagtacg
     4381 tccagccacc gaacttggct cccgagaagc tggcatcccc gctaaaattc gatctttcaa
     4441 acgttgaata tccagtgaaa atagtatttt ggaagtcgac cgatcccaaa aatacagtat
     4501 tgtcgaagtc gaccgatcca aaaatccttc cgctcagatc cagatccccc tccacgatca
     4561 cgccgtcgta atcgaccggc tcgccccgct cgatggcggc caggatctcg ctggctaaga
     4621 ccacctcgcg cgagtcttcg gacgcgccgc tggacgcgcc cgcggccgtc ataagcagga
     4681 gcgccgccag cgccagggcc gggatcgatc tcgtcgatga ttcctcctat gatgaactct
     4741 aggtgtgcct ataaaaagat tcactgttta tccagtcgat tccggctttt atttgaagag
     4801 cagatcggga caatggatga taggatgacg gatggaggcc gaaataggcc cgatgaggcc
     4861 ggcgaactcg ccggagcgga ctaattaaat ataggatatt atacccataa ctacaaaaag
     4921 aggtgcttac atgaaggcca aattgatggc aattctggcg atccttctcc tctctggagg
     4981 gatctctatc gcctcggcgg aagaccaggc tcccgacgtc gccggcgctc atcccgcgac
     5041 agacctcggg gcgatcgggg cgggaggcga ggcgatctcc tccagggccg acctcgcata
     5101 ccccggcgag atcgactggt tcaagttcgc cgtcgagact cctaccgccg agctggtcat
     5161 ctcctctgat atgaccaagg ccctccgctt cgtcctctac gacgagagct tggagtacat
     5221 cgaccacggc gaggacctcc tgaggatgac ggctgaggca gggacctacc tcctccgggt
     5281 agattcgatc ctctcccaga ccggaaactt caccgtctcc atcagcagcg tcatctccga
     5341 ggccgagccg aacgactgca tcaccgaggg gaacatcctc ggtcccctgg ccaatactac
     5401 gatcctctcc ggctcgatcg atcccctcgc cgataccgac ttcttcctct tcgagatagg
     5461 tccggattcg gaggggtatc tcaagatgac gagcgaggcc tacgaggtgg acgaggagga
     5521 tgaggaggag tacgacctct ggtcctattc tgactacctc tcccttgtcc tctacggatt
     5581 caacgagagc gaaggccgct acctgccgat agagagcggc gatggcgaga tggccgccga
     5641 cctctccgcc ggaaggtacg ccctcagggc ccagtctggg agcagcgacc ccatctcggc
     5701 gtacgtcata accctctccc tccaggacct cgaatgtgac gacgagccca acgactctcc
     5761 ccaggatgcc ctggagctgg gggtcctcac tgggggaggg gagctgacgg cgtccggctg
     5821 tataattccc ggcgacgacg tcgactacta cctcctggtg gtggaggagg cgatggaggt
     5881 ggtcgttgag acctcagggg acgaccgcgg cgattcttac ctatacctct acgacgacca
     5941 ggaggaggag atcgattacg acgacgacag cggtgaagac tcctggtcca ggatcgagga
     6001 ggaactctcg gccggaacct actacatcaa ggtagcgtcc ttctggagcg gcgattcgtt
     6061 ccagtacacc ctgaaggtcg cggggaccgg ctctgaagag aagggggagt gatcccgggc
     6121 ggaaggtctc ccttcggcat atccttccag aagacggcgg ctcgcgggcg gggatgcccg
     6181 gccgccgcct ttctgcatcc atctcccttc tacctcaatc tttgcgcccc ggccttctcc
     6241 ccattcatat ccggtccttc gggttcttct tatcgcggaa gagcttctcc ggcccccctg
     6301 cctcaccaga atgccatcac cccgagggct accaggatca tcatcacgtt ccgcaggccc
     6361 gtcgagcaga tcacgagctc ggtcccgatc cgggggccga agatcccggc gtgggatggg
     6421 atgagccagc gaacggccca gcctacgctg gtgatgacgt ttcctatcat gagggtgacg
     6481 accacatcct tcccggagag ctctcccgcc gccaaaaggc ccccagccac ggtgtaggcg
     6541 gcgacgtatc ctgcgaactg ggcggcaatt atgctgatcc ccgccgccgg cagggggagg
     6601 aggccgccga tccccctcag ctgttgggat aataggtcga aggcccccac ctggatcagg
     6661 accgaaacca ggaacatgat ccccgtcgtc atcttcacca ccctcaccac cttggggcgg
     6721 gcagctcgag agcttctctt cagcgcctcc cccaggggcg gctgattctt ccttgggctt
     6781 gcgatcctgc ccctgagatc ttcgagggct atctccgcgg cctcggctcc gggtctcccc
     6841 ggcaggagga ggcggcccgc agccatcacc agggcggtct tcaagagccc caccagcatc
     6901 agaaggccga agtagacgag gcccgtaaac cccaggagag gtatcagcac cgggaggagg
     6961 gccctccagt gcatggtggt ggcggggaag gagttgacga gggaggcgac gaagagctct
     7021 ctcttctcga tgatcccgtc atggtagtag ccggcgagca tcgagtttgc gctcgccgcc
     7081 gagaagaagg ccatcatgaa gctcgctccg gcggcgtcgc tgaggtggga gaaccgggtg
     7141 atcggcttcg cgaggaaggc gatcctctcc gccgcgtcgt agaccacgat cagctccgcc
     7201 agaatgaggc ccaggaccat cggcggcagg gatttggaga ggaggccgaa ggagatggac
     7261 gccgattcga tcaggatctg gaccaactcc gtcatcgccc tacctccccc cctggggcca
     7321 tctccatcat gaggaaaccc tctggtctga accccttatt aagtagcacg gaagagcgca
     7381 cctttcacat actccggcgg cggtctggat tccgaaggtg tccaaaagaa attagtaacc
     7441 ttagcccata ctccggcacc catgaaagag aagtggatga tggcgagcga cggcccctgg
     7501 gaggagatga agccccggat caccaccgcc ctagagtccg gattcgactg cgtggtggcg
     7561 agacaggagg acgtcgatag ggtaagaaag ctcggcgata tcaggatcgc ctccttcggc
     7621 actgagaggg ggggcgagga tatcctcatc gtaggccggg gcggcgaggg ggacgggtcg
     7681 gtacccctcc ccgaggatct cggcgcctct ctggacataa gcctagcccg ggccgctgac
     7741 ggggtggtgg cgggctacgt cgagatccgg gggaagaggt acgaggagtt cgcggtggag
     7801 ctgggcaaga gcgtcgacta cctggtcgtc gtcggcaccg actggaaggt gattcccctc
     7861 gagaacatga tcgctggcct ccagggcgcg aaggtgaaga tcatcagcgg cgtcaagacc
     7921 gccgaggagg cggaggtcga ctttgccacc ctggagaagg gggccgacgg gatcctcctc
     7981 gacacctcgg acctatcgga gatcaagagg gttcagaagc tggcggagaa ggcggggatg
     8041 gcgaggctgg acctatccat cgccaggatc accaccgtca ggcccgtcgg gatgggggac
     8101 cgggtctgcg tcgacacctg caccatgatg acgcctggag aggggatgct cgtcggctct
     8161 cagtccaagg ccgccttcct cgtccagagc gnnnnnnnnn nnnnncggtc cacgcctacg
     8221 tcaaggtcgg tgagaagacc cgttatctat cggagctgaa ggccggggac gaggtcacca
     8281 tcgtcgacgc tgaggggggg acgaagaccg ccgtcgtcgg cagggtcaag atcgagaaga
     8341 ggcctctcat gctcatcgag gcggaggtgg acggggagaa gatcagcacc atcctccaga
     8401 acgctgagac gatcaagctc gtcggcaaag gcggcggacc caaggcggtg acggacctcc
     8461 agccggggga cgaggtcctg gtccacttcg aggggaaggc ccgccacttc gggatgagca
     8521 tcgaggagag catcctcgag aggtgaggtc ggcagagcat cgatgatgcg gttgaccgga
     8581 ggcgggaagg tgatctcggg gaaggatggg atgaggatcg gcgggctcag tccggaggta
     8641 ttggatcgga gcgagcgtag gcaccatcgg cggcggctcg aaatcgggga cctggagatc
     8701 gaggtccccg ccgtcgtcgc cgtcctcagc ggtgaatgcg ctgttgggga ggcttatatc
     8761 gcggagggga tcggggcgga cctggtggag gtgaggctcg acctcatccc cggggacggg
     8821 atgaagatcc tccgggaggt tcgggaggcg acgaccctcc ccatcatcgc caccaaccgt
     8881 tgggctgcgg aggggggcgc cttccggggc ggtgaggacg agaggataga gatcctggcg
     8941 gaggcttcgt cctgggcgga cctcgtcgac gtggaggtta tggccagggg gcgcgaccgg
     9001 ctcctggaga tggtggagag gccggcgatc ctatcctacc acgacnnnnn nnnnnnnnnn
     9061 nnnnnnnnnn nnnnnnnntc ccccgcctcc ctggaggagt gcctggccct tctgagcctc
     9121 ctcctctccg ccccgggccc cacctccctc ctggggatgg gggaggtagg aaggcacctg
     9181 cgggccctgg ctccaatata cggctctgtc ctcacttacg gatacatcac aactccggca
     9241 gccccgggcc agatccctgt gaaagacctg ggggcggtcc tcgacgtgct gatggggcgg
     9301 aagggttgaa gttgatcata attgccaact cggattgagc tttctccctc ctacattgta
     9361 gggaggggga gggcagatcc tgaaccctcc ggactgctag tccattaatg taaaatattt
     9421 gccctaattc tcatcttatt tctcaccatt ttataagaaa attggttcat atttttgtaa
     9481 aatgcgtatt ttatatcatt tcttctctca tcaacatagg ataaaaatag catcattttt
     9541 ttgtaaaatg attccgttaa atttagtctt tcaacgtttt gtaaactcga ttaattggat
     9601 ttaaatgtca ccatatctct gtcttagatg acatagatcc cacaaccttt atatcaagca
     9661 ataaacccac tatagcgcgc ggatatttgt cagatagagg tgagaagatg gataagatgg
     9721 aaggcagttt tg
//
LOCUS       DASM01000087           15980 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_61047, whole genome shotgun sequence.
ACCESSION   DASM01000087 DASM01000000
VERSION     DASM01000087.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 15980)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 15980)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..15980
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_61047"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2734 a         5228 c         5021 g         2989 t
ORIGIN      
        1 gagatccttc tcaccagcat ggaccgggac gggacctacg acggcttcga tatacctcta
       61 accgacgccg tatcgaggag ggtcaggata ccggtcatcg cctcgggggg gtgcggaaac
      121 cccgaccaca tattagaggt cttctcgaag acgggggcgt cggccgccct cgccgccagc
      181 atattccact acggggagtg gggggtcggc gaggtgaaga gccacctcgc cgagaggggg
      241 ataaacgtcc ggatttgaaa aaccccggac gccgggtcgc cctccaggac gggcggcacc
      301 gtcaggttta tagacgccgg accggtctaa tcctggggaa gtctgaggtg tcgaattgat
      361 ctcgctgacg aaagcgccct gcaacctggt cctggagata aaatccttct ccgacagctt
      421 cggctgggcg aagaggctgg agtcgatggg gataaggaag ggacagaggg tccggaagat
      481 cgcctgccag cccttcgggg ggccggtggt gatcgagatc gggggatgct gcgtatccat
      541 ggggaggggg atcgcctcca agatcgacgt ggaggtggtc tcgtaggcgg ccgaccagag
      601 agaggccccc accggggctc tcagatagat cctaaaggat ggatgggggg ccgggggatt
      661 ttagcccgat ataagctctt ttagcttctg gatgctctcc atcgtctggg ccgactcctg
      721 ctgcttctct tcttcgaaat gcctgatcac ctcgtcgatg ggggtgagaa gggagtagac
      781 cttcatcggc ctgcctttgc ccccccttct cacctccttc tcgctcaccc agccgttgtc
      841 gcgcagcgat cgcatcgcga tgctcacctc gggctgcctc aggtttgagc ccatctcgat
      901 gtcccgggag gaggcctcct ccacgttggc caggtaggtt atcaggatgg ccacattcct
      961 ctgcacgccg aggctccgca gggtctcagc aaactcgagg tccttatcgt ccagcacctt
     1021 cactgtgctc tccttcatcg tcgtcaccgg gggattattt taattttcaa tctaacaaaa
     1081 ccttaatcat ataaataatt tatttcgacg ggcattacat taataaaaaa aatgggccct
     1141 tctctggatg gatatgggag atggctcctc tttttctcct gaggtcgcgt caaatttcga
     1201 ggtgctgaga aggagagatg gcacgggacg atgagatggt ttgataatag ggcgggatgc
     1261 gggagagctc ggcagggccc cctacgaggg ccctgcaacc cccctcacgc cgtctatgcg
     1321 aaagaacgtc atgacggccg aagcggacta tcgaaggatc ggatccgtcc gatcggctca
     1381 gaaccgcctc ggtgatcttg caggcctgtg cttctgataa cattcgctgc agtataccgg
     1441 cctcgatccg tcgggcttga agggaacttt cgtctccttt ccgcaatccg cgcagaccgc
     1501 gtcgtgcatc tctctggggc ctctgtcgaa gccgcctcta cgtccaccac gtctatcatc
     1561 cattatattc acgcgccttt ttggtcttcg attccgaaga accgacggtg ctagcgagat
     1621 ccccaaagag gtcatctgcg caagctgata ccgctcctcc cgccatgaag atatacctgt
     1681 tggtggcacc ggagaggtcc cgatgccgcc cccctccagg aggggctttg ggcctctccg
     1741 aacgttctaa atagcccctc tccctccgat agagggccat gactgcgaag cctcccgcct
     1801 tccgatcgta cccccccatc gccaattccc tcctcgtcct cgccctcctc tcagccctgg
     1861 tggcgggctg cctcggtggg ccctccgttg gcatggcccc tccggtttac ggctataggg
     1921 tggtggccgc ctatcctcac gacccggagg ccttcaccca ggggctcgcc ttccgcgacg
     1981 gcctcctcta cgaggggacc ggcctttacg gcaggtcgga gatccgcgag acggtcctgg
     2041 agacggggga ggtgatccga tctaggggcc ttccgccccc cctcttcgga gaggggatcg
     2101 ccctcatcga cgatgagctt ctccagctca cctggaggtc gggggtcggc ctggtctggg
     2161 atctggagag cttcaccgtc gtaggggcct tctcctatcc gacagagggg tgggggatca
     2221 cggcagacgg ccaaaggctc atcatgagcg atggctcctc gaccctcagg tttctggacc
     2281 cagagacctt cgaggtgacg gggtcgctgg aggtggaggc ggatgggaag ccgcttttgg
     2341 ggctgaacga gctggagtgg gtcgacggtc tcatctacgc caacgtctgg aaggccgatg
     2401 agatcgccat catatctccg ggttcgggga gggtggtggg ctggatcgac ctcgccggcc
     2461 tcctctccga ggaggagagg gagagggccg acgtcctaaa cgggatcgcc tacgataccg
     2521 cctcgggacg gctcttcttc acagggaagc tctggccctg ggtcttcgag atcgaggtcg
     2581 tagcttcggg ctgacggaag gttccggcct gggtaatctt aataccgatc aggagcatca
     2641 tgcgctcaga ggtttaagta tgctcagaga tttctcaaat aaattcgcca tcggcgcctc
     2701 cctcgccctg gccatattat tatcggcggg gctcgtccag gcggtagtca ccgatgacgt
     2761 caacttcagc gtcgtagctc cggagggcgt ctccctgggg tcgaacgcca cctctgaggt
     2821 agccggcggg gtggaggatg gggatcaggc gaacctgacc ggacccctct ccgaggtcgg
     2881 cggcgaggcc gcggccaacg agacggctcc gccgacggcg gccggggctg cggaggtccc
     2941 agccaccgct gatacggcca acgaaacgct gcctgaggcc gccaacgtct ccggggtgga
     3001 gattaaaccc gaggcggcgg gaggggtgct ccgggccgtc tgcctcaacg gctgcagcta
     3061 caccaccatc caggccgcca tcgacgccgc cggagagggc gacgtggtgg aggtggggag
     3121 cggaacctac cgtgagaacg tcatcgtcga taagagggtc acaatccggg gggttaacac
     3181 cggcgaaggg gtgccggtgg tggacgccca gggtaacggc agcgccgtcc tcctgaaggc
     3241 ctccggggtc ttcctggagg ggctcaccat caccaacgcc ggcccctacc ccagcgccgg
     3301 gatcgaggtc gtctcaagcg acagcttagt ggccggctgc gccgtcatga acagcaactg
     3361 ggcggggata tacctgaagg gatctgccaa caccaccatc tcacggtgca tctcctccaa
     3421 caacggcaac gacggcatcc tcgtcttcag ggccactgga aacctgatct ccgagagcgt
     3481 cgtcagcaac aacctcgacg acgggatagc cctcctcagc tccgatgaca accggatcga
     3541 agggaacctg gtcgcgaaca acaccgacgt ggggatcttc ctcgacacct ccaaaaacgc
     3601 cgtcgtgacc ggaaacgtcc tcagctacaa cgcgaagggg atcagccttc tcaactccgg
     3661 gatcgaccgg gtcggtccaa accggttctt caacaacacc gtaaacctcg agggggtata
     3721 gaccggcctt tagaggccgg ggccctccca tttctaactc tatttttgcg atccaggacc
     3781 ctcaaccccg gagggactat ccgggcatct ccccgtcccc tcccgacggg cggagatggg
     3841 ctggggcgga cggccgttgc cttcgaaatg gatttaaccc ctggatgcca gataacatcc
     3901 tgccatgcaa gattattccg tcaatcagtt cttagaactc gccaagactc cgaagataga
     3961 gatcgaggtc tgcgacgtca ccctgagaga cggtgagcag atgcccgggg tcgtattcag
     4021 gcccgacgag aagatcgaca tcgccatcaa gctggacgag gtgggggtgg agatcatcga
     4081 ggcgggcttt ccggtcgtct ccgaggcgga gatgagggcg gtccgggacg tctccaacct
     4141 cggcctcgac tccaagatct cggtcctctc ccggtctgta cccaaagacg tcgacgccgc
     4201 cctcgcctgc gacgtcgaca tggtcagcgt cttcatagcc acctccgacc tccacctgaa
     4261 gtacaagctc cacatgacct gcgaggaagc gatcaagagc gctcttgaga cggtggagta
     4321 cgccaaggac cacggcctca tcgtccggtt ctcagcggag gacgccacca ggacggagtt
     4381 caatctcctc aagcggctct acaaaaaggc cgaggagtgc cgggccgact acgtcagcat
     4441 cgccgacacc gtggggatca tgaaccccag gactgtctac ttcctggtaa gcgagatcaa
     4501 gaaggaggtg aagatcccga tatgcatgca ctgccacgac gacctggggc tcgccctcgc
     4561 caacaccctg gcggcggcgg aggcgggggc gaagcagctc cacaccaccg tcaacgggat
     4621 cggcgagagg agcggaaaca cccccctcga ggagctgatg gtcgccctcc gggtccacta
     4681 ctccgtcgat aggtacgaca ccacaaagct cacagacctc tcgaagctcg tccagagcta
     4741 ctcgggggtg atgatgccga agaacaaggc cgtcgtcggc gacaacgcct tcgcccacga
     4801 gtcggggatc cacgtcgccg ccgtcttgga ggagccccgg acctacgagc tctactcccc
     4861 cgagatggtc gggtcggcga ggaggataat catcggaaag cacaccgggg cccgggccct
     4921 caagtacatc accaagaaga tgggctacga cctgaagagg gacgagatct gcctcctcgc
     4981 cgagcgggtt aagagatgca gtgagttcaa gcggcccata tcctgcgacg agctccggaa
     5041 gctgatccac gacctggaca tcgagatcgt ctaccccgac ctctgagggc gttccctccg
     5101 gtccatcgtc ccgaaagacc cgcgggcctg gccgctcctc ccatgcgggg gcctgaggcg
     5161 agagcacccc cgatcggtgc cgcccccttt acagtccgag ggggatggag ggctgcacct
     5221 ttctggcttt tctgggatct tggggtatac tacgctggaa tttgacgtct aattctgaga
     5281 ggtcagaaat caccaccatc ttaaaataga cggcggagta tgcgggccaa aattgcaggc
     5341 ctcggatgag gcggagtagg cataggtgag agtcagggtc tttctcaggg tcttcggcac
     5401 cctcttgaag ctgctggggg ggctgatgct cgtccccgcc ggggtgtcgc tcctctacgg
     5461 cgagatcgag ggggtgatag ccttcctcct acccgccctc ttcaccttcg ctataggcct
     5521 tttgatggcg atcctggggg aggaggaggt cctcaccaac aaggaggggt tcgccctggt
     5581 ggccttcgga tggctggggg cggcagccac cggcgccctc cccttcgtcc ttttggggct
     5641 cagccccata gacgccctct tcgagtcgat gtccggcttc accacgacgg gggcctccat
     5701 cctctccgag agcggggccg atggcctctt cctaataaac gccaccgccg cgagctcaag
     5761 cctggcggtc accctggtcc agatcgcctc aaaccagagc ttcatgccat ccgccatcag
     5821 tccatccacc tttttggcct cgaacgcatc ggccctcaac gatacgatcc tcaacgccgc
     5881 ctttgccgga ggcctctcgg gaaatgcgac gaacctgaca tcaaatctga tcgagcccac
     5941 ttataggggc ctcctcttct ggaggtgctt caccaactgg atcggaggca tgggaatcat
     6001 cgtcctcttc atagcgatcc tccccaacct gggggtagcg ggccgccagc tcttcaacgc
     6061 cgaggttccg gggccgacgg aggatctgat aaggccccgg atcaagcaga cagcgaagat
     6121 cctctggggg gtctacatgg tcctcacggc ggccgagttc ctcctcttga tgctcgccaa
     6181 gatgccggcg tatgatgcca tctgcaccac cttctccacc gtcgccaccg gcggcttctc
     6241 cccccgggcg gagagcatcg cctactactc cggccccggc tacaacggcc agctcataga
     6301 ggtcgtcgtg atcctcttca tgttcgtctg cggggcgaac ttcgccctcc actatcggac
     6361 gatgtacgtc agcagaaaga gcctcataaa agatccggag ttcaagttct actcgctcat
     6421 cgtcctcgcc tcgatcctcg tcatcaccct catcggcggc atcgacgaga gcctcccccc
     6481 ggaggggctg gatctcgaag ggttcgatct ggcgagcctc cgcctccgga ccgccgcctt
     6541 ccagaccgtc tcaatcatca ccaccaccgg cttcgccacc gccgactacg acctctggtc
     6601 cagccctgcc aggctgatcc tctttctgct gatgctcagc ggcgcctgtg cggggtcgac
     6661 ggcgggggcg atgaaggtcg tccggatcct cctcgccctc cagtgctgcc gccgggagct
     6721 gttccggacc ctccacccca aggcggtgat ggcggtgaag ctgaagggag cccccgttcg
     6781 ggaggacgtc ctccgctcga tcctgatatt catggcgata tacatcctca tcttcgccat
     6841 cggctcggcc ctcttcgccg cggtctgcac cctcgctgag gaggagacgg ggacggagga
     6901 tcggatctat ttcgtctccg gggcaggagt cggtctagag gtggtgggcg ggatctcccg
     6961 cggctgggcc ggggcggagg gaccggagga tgggatctgc tttggccgtg gcggcggggg
     7021 gatcgacatg atcagcgcct gctccgccgt cgccacctgc cttggaaacg tcggccccgg
     7081 cctccaccga ttcgggccta cggacaactt ctcggaggtc ccggggattg gaaagctcat
     7141 cctcatcctc tgcatgtgga tggggaggct tgaggtcctg acggtcctcg tcctcctgat
     7201 ccccgacttc tggaagggct gagacgatcg atggcgaggc ggacgggcgg atgggatctt
     7261 catcgggggt cggggagggc gggagatccg cgaaattagc ggacggataa aatttcgaaa
     7321 caaccaccat ggtaaaatag gggacgtagc ttctttaact ggcgaatttg aggtcggctt
     7381 cggatgatct ggacgtgaga tgggtgagga taagggtctt tctcagggtc ttcggcaccc
     7441 tcttgaagct gctggggggg ctgatgctca tacccgctgg ggtgtcgctc ctctacggcg
     7501 aggtcgaggg ggtgatagcc ttcctcctac ccgccctctt caccttcgct ataggccttt
     7561 tgatggcgat cctgggggag gaggaggtcc tcaccaacaa ggagggtttc gccctggtgg
     7621 ccttcggatg gctgggggcg gcagnntaga cgccctcttc gagtcgatgt ctggcttcac
     7681 cactactgga gcatccatcc tctcggaggt cggcgccgac ggcctctggg tgatgaacgc
     7741 cacggctgcg aagagcagca tcgcggcctc catcgccgct atggccttcg gccatgggac
     7801 ggggacaatg gccttcgatc cagcctggct tgggggatca aacctgtcgc tcctgaacga
     7861 gacggccttc aactcctccc tgggggggag tggggggggg ggcgaggtcc tnntggctgg
     7921 gggggatggg gatcatcgtc ctcttcattg cgatcctgcc gaacctgggg gtggcgggcc
     7981 gccagctctt caaggccgag gttcctgggc cgtcggagga tctgattcgg ccgcggatca
     8041 gggagacggc gaagatcctc tggggggtct acatggtgat aaccgccgcc gagtttctcc
     8101 tcctgatgct cgccaggatg ccggcgtacg atgcagtatg taccaccttc tccaccgtct
     8161 cgaccggcgg cttctccccc cgggcggata gcatcgccta ctacggcggc cccgactata
     8221 acggcccctt catcgaggtg atcgtcatcg tcttcatgtt catctccggg gcgagcttcg
     8281 ccctccatta tcggacgatg tacgtcaaca ggacgagcct gatcgaagat ccggagttca
     8341 ggttctactc ccttctggtg ggcgccgtca tcctcatcgt cgttctcatc ggcggggtcg
     8401 acggcagctt cctgggggat agccccgagg ctgagggggt ggggctagcg ggccagcggc
     8461 tgagggccgc cgccttccag accgtctcca tcgtcaccag caccggcttt gccaccgccg
     8521 actacgacct ctggtccagc cccgcgaagc tgatcctcct gttcttgatg ttcaccggag
     8581 cctgcgccgg gtctacctcg ggggggatga agctcgtcag gatcctcctc tcggtgaggt
     8641 actgccgccg ggagctcttc cggacccttc accccaaggc ggtgatggcg gtgaagctga
     8701 aggcctcccc cgtccgggag gacgtcctcc actcgatcat ggtattcatg gccctttaca
     8761 tcctgatctt cgccgtaggg tcggtcctct tcgcctcggt aacctctctt ccgggggtgg
     8821 cggcgacgtc tgaggagctc gtctgcttcg tctccggcga tggcgagaag gtcgtatgct
     8881 tcggtccgga tggctggaac ggctccaggg agctcttcgg ctcggaccct aagacctccg
     8941 gaggtgtcgc ctgcttctta cccaccgaga acggcaccag gaggatagag gtggaccgaa
     9001 ggcccatcaa cctctcggag gtctactcga agggtccggt ctcgggtgat ctcgggtcag
     9061 gcttcagcag cgatatggac gtgatcagcg ccgcctccgc cgtcgcctcg gccctcggaa
     9121 acgtcggacc cgccttccac cggttcgggc cgacggacaa ctattcggag gttccaggga
     9181 tcggcaagct gatcctgatc gtctgcatgt ggatagggag gcttgagatc ctgacggtcc
     9241 tcgtcctcct cgtccccgac ttctggaagg attgagatga tgctggaagt atctttgagg
     9301 aatggggggt cgtgaagctg aagggcgaga tgaggctcgt caccctcgcc gagaactcgg
     9361 cggggctccg gcggggcgtc ctcgccgagc acggcttctc cgcttacctg gagttggggg
     9421 attccagggt gatcttcgat gcggggcaga ccaccaccgc cgtctccaac gcccgggctc
     9481 tggggataga tctggaggga gttcccatcg ccctcagcca cggccattac gaccatacag
     9541 gagggctggc cagcctccta gagaaaaccg gccctgtccc ggtttacgcc catcccgacg
     9601 tcttcgtctc ccggtacacc cggcggggcg gggtcctcag gcagatcggg atccccttca
     9661 ggagaggtga tctggaggcg gccggcgccc ggttccagct ttcccggagg gcagaagaga
     9721 tattcccggg gctctggctc acaggtgagg tcccaaggat cgccgggttt gaggggcgaa
     9781 gcgaggatct ggtcgtcgtc tcggaggggg gcgagatcgt cctcgatccg atccgcgacg
     9841 accaggccct catcgccgtc ctggagaggg ggatcttcgt cctcctgggg tgcgcccatt
     9901 cggggatgat cagcactctc ctccacgcga agagggtcac cggctgtgac cgggtcctgg
     9961 gggtcgccgg ggggacccac ctcggcttcg gcggcgggga taagcttcca aggacgatcg
    10021 aggcgctccg gtccctcgac ctcgaggtct tggccccctc ccactgcacc ggctttgccg
    10081 ccgcctcggc cctggcggcg gccttccccg accggttcgt cgagaacagc gtggggacgg
    10141 agatcatcct ctgacggctc ccgtccaaaa aagggcgatg gaagggggaa cgggggtttg
    10201 gcgtagtcta aggctcatgg tcctgatggg tgttggttcc ggccttcggc tcagccgggg
    10261 gtgccgaacc gggggcacct cccccgcgcc accaccaggg agaccccggg cttcgccagc
    10321 tcttcggcca ggacctcctc gatatcaccc cgatcctccg gaaggtcgat gctcctggca
    10381 tcagggacta tcgtcctcag caccggggag aggtcgggga cgccctggcc tcccgtcatc
    10441 gccgcgacgc cgttatcgag gaggaggacc agcacctctc ttctgttatt aacggcgtcg
    10501 atcagcccca aaaggccgga gtgggcgatg gcgtagtcgc cgatgagggc gacccccttc
    10561 tccccgaagc cggaggcgac ggccaccgag gatccgaggc cgtagacgac gtccaccgcc
    10621 ggggggtccc ggaccgcccg gatgctgcac ccggcgtccc ccgccaccgg gacccgaagg
    10681 ccaaaaaccg cctcgtagag gggtcggtag gggcagtcgt cgcagacctt ctttgaggcg
    10741 gcccgatcct cggacctctc gggcttttgg gcggggcccg gctcctcccc cgagccgatc
    10801 atctcgatcg ccctcgccag gtcccccgcc tccatcctcc cccaggggag gtggcaggtc
    10861 agcttcccga agataccgcc gcagaccatg agcctcgcct cgataaaggg ggtcggctcc
    10921 tctgccacca ggatcttatc gtgctccgat acgaactccc ggatcgcctc tagcgggagg
    10981 gggtgagaga agccgacgac caggagggga aggtcggtct tggatgcata tcccgcgggc
    11041 ctcccggagg ctatgatccc gatatccccc ccgcctcccc tcaggaggtt gagggaggac
    11101 ccctccgagg cggacctcat cgccgggagg gcttcggtcc tgtacctctg gtggcgcccc
    11161 ttcgccgtca gatcccagat cgaccggtcg aaggtcggag acctcctcac cttcctctga
    11221 gggggaggga cccggaaccc ggcggggtcg cggctcgata caccaccccc tcctccgaac
    11281 tcgaaggtcg tcctcaccat cgccggcgcc ctgacctctt cggatagggc gtaggcctcc
    11341 cggagggcgg acccgaggag gatggggctc gccgggtcca ggacggggac ctcgcagagg
    11401 gggccgtagt tcctcacgtc catctccgcc tgagaccccc gggggcccac gtcctcgccg
    11461 gcgaggacca ccagccccgc ccctatggtg tgggtcgggg cgatggcgag ggggtcggcg
    11521 aggaggttta tgcccaggtg cttcaccagg acgatcgccc tcctcccgga ggcggagctc
    11581 cccagggcca cctccagggc caccttctcg ttcgtcgcca tctcggcgcc ctcgcccagg
    11641 gcggggacgg cttcggtgat ggggtagcca ggaacgtaga tccggacctg gacgttcagc
    11701 tcctccaggc atcccttcag gacctccacg gctgcgggcc ccccaatccc ggagccctcc
    11761 cctccggcga tctctctttc tccattcatg ccgacgactc ccgtctcctc agaggagggc
    11821 ccctctcggg gcttcagccc ctccatcctt tccaggatct caccctttcc ctcattctcc
    11881 gggcctcacc gacgcgaggg gccttccgca gaaggggatc gccgcaagag cccctatcag
    11941 accccggccg gcgatgacga cctccgtccc ggtccgcgcc gccgtctcga tcgcgaggtc
    12001 gtaggcgatc cgctcggtcc tgcagagttg ggagtactcc atcaggggcg aggggtcaaa
    12061 cccccggtag accgccatcc ccgtctcttc ggagacggtg taccgctcca gaagctcccg
    12121 gaannggacg agggcgtggg agacgtacct cgcctcgggc cggtcgacct tcgcagcgat
    12181 gttgtggacc agggaccacg tcgccccctc cgtcttagag tcggtgtcgt cgacgccgac
    12241 ggctatcctc tccctccggg gaaggacgat cgtcccccgc cccaccttcc ccccaccgga
    12301 ttcggagacg ctagatcgga ggacgtcccc ggccctcgat ctggagaccg tcgcgccgac
    12361 gccgccgccc ccgaggccgg cgtaggttat ctccacctca tcgcccctca ccgccacagc
    12421 ctcgatcccc gccgaccgcc gggatgaggc gagggcgagg tcggacctcc ccgcccggac
    12481 gcggtagcgg aaccactccc ccacggacct ggcctccacc accaggggcc ctttagagta
    12541 gtggtggagg gaccaggccc cgcccccgaa gcaggaggag cgctccagga tctccacctc
    12601 ctcgccgtcg gagctggcca cggcgtggat ctgtcggtag acgacagcat agggatcttc
    12661 aagagcgagc acggatcaac acctcgaacc agcagatcgg aatcatcggc ggagaggtta
    12721 ttggttctgg tctataagat ctcggatggt ggggcctggg gcatcggccc acttcctccc
    12781 ccctcctacg ccttcatata ctctctcagg acgacggtgg cgtaggagcc cgggggaagg
    12841 tcgaatttga ggagggcaga gctcccttcc tcgacgacca cgacggggtc aaccctgagg
    12901 agggcggccc tccggctccc cggagagccg aggccggggt ttgcatcgac ccgaaagtcg
    12961 gatagctcga ccccctcctc ggcgaggacc gccctctcga tctcccccgg gaggcctccg
    13021 gcaaactccg atccgtatcc gaagaggggg agggtcacaa aggccctccc cctatcggcg
    13081 aggcggttga cggccgagag gttcgacccg tcgacggcct ggagcttatc ggggttgggg
    13141 aggccgtcct tcgagaagca gaccacgtcc ccctccaccg cccgccccag ggggaggccg
    13201 gacttcatcc tccgggatag gatcctgtta tagaggaggg actggtaggc gtggacgaag
    13261 agcctccgga ggttctcggc gaggacgtcg aaggatcggg cgaagtcgcc ctcctcctgg
    13321 aggcgggcca tcatcgccct ctcgtaccgg agccggaggg ggtactcctt cagggccagg
    13381 ccgaagtccc gggcggcgag gagcctctcc ctcgccgccc gggtcgactc cggctccccg
    13441 gggaagggga gggcgaggta gatcatcgcc gcccccaccg ggtctcccct caccagggcc
    13501 tctccgacga ggtgggtgac cggcctcacc tccccgaacc tctggacccc gaagtagttc
    13561 ggcaggccgc ctaaggcctc gatctccccg ctgaccgcct ccagccgccg ggccgcctcg
    13621 ccatagtcga ggtcgatctc ccggatccgg acggcgaacc ggtttccccg gaggtcgcct
    13681 agggataccg gccggtccga cctccccagg gccctgatcc tgacttcggg gaggttcgcc
    13741 cggaccagct ccccctcctc gaggccgtat atgctcatcc tctggcgggt gacggcgttc
    13801 ttgtccttcg tccccgccca ggagaacctc ttctggctga tctggagctg gcgggagagg
    13861 tccctcacca ggtggtgggt ctcccagccg gccttctcca cctccacgat gaggtagcgt
    13921 cccctcccat acctctggtc gagggccagc tcctctacgg cgaagtcccg gacctcaacc
    13981 cggagccgcc cgccgcatcc gggggagctg gtggcgtaga gctccatccc cagggaccgg
    14041 tcgaactctg aggcctccat catctcctct cctcctctcc tcagacgagc ttcaggccgc
    14101 cggtgatccg atcgatctgg gcctccgggg cggggcccag gcctagggcg gtgactgttc
    14161 cgggaggtat ctccgtcagg ccggcgtcta tcacgatggc ggccgccacc cccgccctct
    14221 ccgcctcgtc cctaagccga aagacgtcct ggagggtccg cgccttcagg acgatcttcc
    14281 tctgcccctc ctccttccag tccctgacga tccgcttatc cgcccgctcc atggcgagga
    14341 ccgcggcgtg ggcgacctgg accgccagct tgccggagga gagcttcaga tcttcccgga
    14401 cgatgatgca ctgcttgaac tccatgatgt cgacggttag aaaccccata ggataaaaga
    14461 gtagacgccc ctccgccccg atccgcgggg cgccagcccg ggggggcgga gggggccagg
    14521 agaggggagg aaggctcgat cccgtcacgc ctctcccgtc atcaccttga cgcagagggg
    14581 gcttttgacg atgtttccga gcttcgcccc gatcaggtac tcgagattct tgtcggcggc
    14641 gatgtccagg atcctctggg tgacgacgcc gtcgaagatc gctccggcca cgtcgccgtt
    14701 ggactccttc agttcttttg ccaggtccct cacggccacc tcccggatca tgttctggtt
    14761 ccggtcgaag aggcgggcgg agagggtgcc gttgagggcc tccaggtggg ggacgaacca
    14821 ctcccccgcc tcctcggaag cctcctcctc tatggcgacg gcctccggct cggcctcgaa
    14881 ctccacctcc ggctcaggct cgtggacctg gatctgaggc gccgcctcca ccgacctctc
    14941 cgacccccgg ctgatccgaa cgggccgctc ccggatgagg ctcttcctcc gggtggtgac
    15001 gtcccgccgc tgcttcttag agaggggctt gatcttgtag tagtccagga cctgctcgac
    15061 ggggatcttc tggcggaggg ccctcacgat ctccttctgg gacatatcct cgacgcactt
    15121 accctcgggg gcccgggcga cgtagtctat gtcggcgacc tggaggagct cctttacgat
    15181 cagctctccg ccccggtccc cgtcggtgaa ggccgtcacc accttcttgc tgcagagctc
    15241 ggcgatggtg ggggggacgt tcgtcccgcc gacggcgacg gcgttcttga tcccgtaccg
    15301 gaggaggttg aggacgtcgg ccctcccctc caccaccagg atggcgtcgg agtcgaggac
    15361 gttggggcct gcagggagcc gatccggccc gatgtgggtg atctcctcca cccggacgga
    15421 ctgctttatc tgctcggtga tcccctgggt gtcgaggatg ttctcgtcga acatctccat
    15481 gatgatctgc tttgccctct ccacgacgta cctcctcttg gaggcccgga cgtcctcgat
    15541 gttggagatc ttgatcctgg cgatgcaggg gcctaccctg tcgatcgtct ccagggcgga
    15601 ggcgaggatc gccgtctcca ccttgtcgaa gctggagggg attgagatac tcccggagga
    15661 tttgccgccg ctggaggcga tctggacgtc gatcctgcct atccggcccg tcttctggag
    15721 gtctcggagg tcgaggtcgc ttccgaggag cccctccgtc tggccgaata tggcgccgac
    15781 gacgtcgggc ctctccacga tcccgtctgc nntttagtgg tatcaacatt ctgcatggcg
    15841 gacacctcaa attttaaaat agacagataa atacactcgc ggctaagagc agagctaacg
    15901 gggcgcaatg cacgacttcc gctcggagcc taagaacctc agcatactgt ttttcagagg
    15961 atgatggtga aggtgtatct
//
LOCUS       DASM01000088           11835 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_109914, whole genome shotgun sequence.
ACCESSION   DASM01000088 DASM01000000
VERSION     DASM01000088.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11835)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11835)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11835
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_109914"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2365 a         3522 c         3459 g         2489 t
ORIGIN      
        1 tgttgcccag ggtcaccggg atcagattgt tcaggatgaa gttgccccag gtgagcttgg
       61 cgtagttggc gacggtaata gccgccgggg tcgcattggc cagcgcgctg gaagcccaaa
      121 acgcatccgg agcgaagtcc ttgatcagca ggcccatggg gacgaagtac atgttggcga
      181 tgctgtgctc gaagcccgcc gccacgaatg cgctgatggg gaagatgatc gccaggatct
      241 tgtcgatggt gctgcgggcg ctgtagcaga gccagacagc catgcagacc agggcgttac
      301 acatcacccc caggaagaac gcctggatgg gatcgagcga tgccttggca ttggcaatgt
      361 tcagggccgt gaggccgatc gcgccgttgg agaaggtgta ctgcctggcc agcagcatga
      421 tgatggcggt caggatcgag ccaacgaagt tacccaggta gacgatgccc cagttgcgca
      481 gcaactggaa cgtgctcacc ctgcgcgaca cccacgccat gacaatgagg ttgttgccgg
      541 tgaagagctc ggcgccagct atgaccacca ggatcaggcc gaggcagaac accaggccgc
      601 ccaacatgcg gttcacgcca taaggcagcg ccccgccgcc cgtccacacc gtcgtcgcgt
      661 agatcgcgcc gacggcgata aacgccccgg ccagaatcgc caacgcgaac atcgtcttga
      721 agtccagcga tgctttcttg atgccaacgg cctcggcccg ggccgccatc tcgggcggca
      781 tgagcgagtc gatagtgatc tctgaagttg gcatattacg gtttcctccc tcttctggat
      841 gaatgatatc tcaaacgacc tcttgccagc cggatcttcc ggccgccgga cgtcgcctgc
      901 accctaacag gcccccaccc caaccggggg gactttcgcc acccgcactg cgcatgcttt
      961 gtactccggg atcttcgcca ccggatcgta agccgaattg gtcagcaggt tggcggcgct
     1021 ctcggcgaag tggaaggtca tgaagacgac ccccggcgag gacttctccg tcagcctcgc
     1081 cttcgccctc accttccccc tccgggagga gacctccacc aggtctccgt ccccgatccc
     1141 caggacctcc ccatccccgg ggtttatctc caccagctcc tcgccccgga ggtagttcag
     1201 ccccgccacc ttcctcgtca tcgtcccggt gtggaagtgg cagaggaccc tcccggtggt
     1261 gaggatgaag gggtaatccc cgtcgggaag ctccgccgag gggcggtaag ataggggcgt
     1321 gaagagcccc ttcccccggg tgaacccgtc tttgtggagg tacctcgtcc cggggtggtc
     1381 gggtccgggg cacggccact ggagccccgc cccctcgagc cttcggtagc tgatcccgcc
     1441 gtagctgggg gtgagggagg cgatctcctc catgatctcc gaggggtggg agtaggagaa
     1501 ctgatccggg taccccatcc tcctcccgat ctccgagatg atcctccagt cgggcctcgc
     1561 ctcccccggc ggcgggaccg cacgcctcac cctctggacc cgccgttcgg tgttggtgaa
     1621 ggtcccgtcc ttctcggcga agctcgccgc cggaagaacc acgtcggcat gcatcgccgt
     1681 ctccgatagg aatatctcct ggacgacgag gaagtcgacc ttctccaggg cctcgacgac
     1741 gtgggtggag tcggggtcgc tgatctgggg gttctcgccg atgatgtaca agacatctac
     1801 ggccccgacg tggatggcgt tgaagatctc catcagggtg agccccggcc gcttggggag
     1861 ctctctatcc cagcgggcct caaacttcct cctcaggtcg tcgttcgtca ccgcctggta
     1921 gccggggagg acgttgggga gggcgcccat gtcgcacgcc ccctggacgt tgttctggcc
     1981 ccggaggggg tttatgccgc tggagggctt tccgaggttt ccggtcatca tcgccaggtc
     2041 ggcgagggcg aggatgttct cggtcccgtg ggagtgctgg gttatcccca tggagtagac
     2101 gatagacgaa gggccgtttg aggcgtagat ccgggccgcc tcctcgatca gaccgacctc
     2161 gacgccggtg atggcggccg cctcctccag ggtgaccccg gccagggatt cccggaactc
     2221 ctcgaacccc tcgcaccgct ccccgatgaa ctcctcgtcg aggagcccct cctccaggat
     2281 caccttgcag agagcgagga cgagggggac gtccgtccct ggggtctgcc tcagccaaac
     2341 gtccgcaaat ttacagagct cgatccaccg ggggttggcg acgatgatct tcgcccctct
     2401 cctcttcgcc tccttgatcc tgagggcgat gacggggtgc tgctcggtgg tgttcgaccc
     2461 tatgatcagg atgcaccccg cgtccgccag ctcctcgatg gagttggtca tcgccccact
     2521 cccgaaggcc gtcgccaggg cggcgacggt ggaggcgtga cagaggcggg cgcagtggtc
     2581 gacgttgttc gtctccatca ccagccgcgc gagcttctgg gcgaggtagt tctcctcgtt
     2641 ggtgcatttg gcggaggaga ggagggcgaa gcggtcacct ttggcgcggg agaacctctc
     2701 tacgacgacc gagagcgcct cctcccaggt ggcggggacg agctcccctc ccttccggat
     2761 gaggggggtc ttcagccgct cccggctctc gacgaagtcg aggccgaacc tccccttgac
     2821 gcagagctgg ccgtggttcg gggtctcgcc cctcttcccc cggatcgtgg cgatgtgtcc
     2881 gtccagaacg ccgagatcca tgacgcagcc gacgccgcag aaggggcaga cggtggtcac
     2941 ggtctcctcg ggcctccccc acctttcggc cctggcgaag agcgcccccg taggacagac
     3001 gtcgacgcag gcgccgcaga acttgcactc cgactcgaag agggagaccg gtccgatggt
     3061 gtggtggtga tggtagttga cgtcgaaggt gaggaccccc tcccccctca cctcctggca
     3121 ggtccggacg caccggctgc agaggatgca gaggttgtag tctcggtcga agaagggctc
     3181 ccggaggacc ggctggtttc ggtagctcgt ttcgtacctg atcctctcca ggccgacgta
     3241 ctccaccaca tcctgcagct cgcatcggcc gtcgttggga cagtacttgc accccaccgc
     3301 cgcgggaaac ttcctcatgc actcccggag gtctgagcac tcctccttcc ggtcgcagaa
     3361 gaggcagctc gtcgggtggt gggtgagggc gaggatcagc tccagggcgg atcggcgcat
     3421 ctcctggagc tcctccgtcc cggtcaggac caccataccc tcctcggcca gggtggtgca
     3481 ggaggtgggg cggaggctct ctccctcgat ctcgacgatg cagagtttac acgatccgat
     3541 gggcgtcagg tccggatggt cgcataaggc cggtatgtag atcccgaccc ctcgggcagc
     3601 ttccaggacc gtagcccctc tcctggtcct gaccttccgt ccatcaatgg ttagggcgat
     3661 ctcgtccact ttcaccaccg ctcagtttta ggagttccgt cgtcatattt ctaagttgct
     3721 ctcgattttc cgcttgaaat ggaacttcta gatggtgtat ttgaatcaaa cggggcgacc
     3781 gccgccttga tggataacat atatgtactg atgggccgga aagatctctc cggctcgttc
     3841 gggtctcctc caaaagagtt tagaccggtg gcgatcgagg gacgccggaa ggcgaaaacc
     3901 gaaaagattt aaatatcctg gattaggttg aatgccacag cccatcgaag ggaggatttc
     3961 gatggcagca agccaggggg ccccatctta tcgccgcttt cgtctaggca aagagggctc
     4021 ccaccacgaa ggtgacggcg ctgggaaggc gtcatccgaa gagccgggag agcatcagga
     4081 tatcgaagcg ggataagatg tctgataata agaagaagct gaacctgaac cgggtatcga
     4141 tgcccaagca gtctccagag gagcggcggc ataacttctc cgaggtggct ctcggctacg
     4201 ccgaggatca ggccgcagaa gaggccgata ggtgcatcca gtgcaagaac ccgagatgta
     4261 tggaaggctg tccagtgggg atagatatcc cggcttttat aagggcggtc cgggatggcg
     4321 agatggagga ttcggtccgg atcattaaag ccaaaaacag cctccccggg atatgcggac
     4381 gggtctgccc ccaggaggtc cagtgcgaga gccggtgcgt cctggagaag aagggcgccc
     4441 ccatcgccat cggccgtctg gagaggttcg tcgccgactg ggagtcgaag aacgccctcc
     4501 cggatcgccc ccgccggtcg tcggaggccc caacggggaa gagggtggcg gtcgtcggag
     4561 ctggccccgc ggggatcacc gctgccgccg acctcgccag gatgggccac caggtcaccg
     4621 tctttgaggc gctccacgac gccggggggg ttctgatcta cgggatcccg gagtttcggc
     4681 tccccaaggc gatcgtccgg tcggaggtcg agtacgtcaa gagcctcggg gtcaccttcg
     4741 aactcgactc ggtggtgggg aggctcgtcc aggtcgatga gctcctgaag aacggctttg
     4801 acgccgtatt cctcgggatc ggcgccggag cgccccgctt cctcggcgtc cccggcgaga
     4861 acctcaacgg cgtctactcc gccaacgagt acctcacccg ggtcaacctg atgaaggcat
     4921 accggttccc cgagtacgac acccccatca ggcaggggaa gagggtggcg gtcgtcggcg
     4981 gcgggaacgt ggccatggat gcggcgaggt gcgccgtaag gctgggggca gaggaggtcc
     5041 acgtggtata ccgccggtcg gaggcggaga tgccggcgag gctcgaggag atcgagaacg
     5101 ccaaggagga gggggtgatc ttcgacttcc tgacgaaccc cacccggttt gtagcagacg
     5161 accgggggag ggtggcgggg atggaggtgg tgaggatggt cctcggcgag ccggatgaga
     5221 gcgggaggcg caggcccgtc gtcgagaagg gctccgagcg gatcgtcgac gtcgacaccg
     5281 tcatcatcgc cgtcgggacc accccaaacc ccctcctccc ggggaacacc ccggggctcg
     5341 agacgacccg gtggggcacc atagtcgtcg acgacgccgg gaggacctcc cgggaggggg
     5401 tctgggccgg aggggacata accaccggcg gcgccaccgt catcagcgcc atgggcgccg
     5461 ggaagagggc ggccgaggac atcgaccgct ggctccgaga ggatggccct tggtcccgct
     5521 gaccgcccat ccggccgggg ccgttccccc agctgaggcg ggacctctca gcccctcgag
     5581 ctcggccgga ggttggaccg aggctccggg ggcctacagc cttcattttt aagatccgtc
     5641 tttgagatga gatagatttc agggagaatg atcgatggga ttcaaatcgc caaaggagac
     5701 gacctgctcg gtcgacaaca tcggatgcaa caagatcggt atggccatgg gacctcttct
     5761 cgctctcggt tttctcgccg gagccttcat cgccttcgga gggctcctgg cggtgatcgt
     5821 cggcgggggg gtccccgata tcaagctggc aaatcccggc ttgcagaagt tcatcttcgg
     5881 ggccgtcttc ccggtggggc tcatcctggt ggtggtggcg ggggcagagc tcttcaccgg
     5941 caacaccgcc tgctgcgtcc cggcggtcct atccgggaag gcctcctggt cggggctcgc
     6001 gaggaactgg acgttctctt acgtcggaaa cctcatcggc tcgctcttcg tcgtcctctt
     6061 cctcacctat atgaccggcc tcttcgcctc cgatccgtgg ctttcgagca tcaccggcat
     6121 cgctgaggcg aaggtctccc aggggttctg gcccctcttc ttcaagggga taggatgcaa
     6181 ctggctcgtc tgtctcgcgg tctggctcgc catcgcctcc gacgacgtcg cgagcaaggt
     6241 ctttggggtc tggtttccga tcatggcctt cgtcgccata gggtttgagc acagcgtcgc
     6301 caacatgttc ttcatccccg cggggatcat gtacggggcg agcgtcacct gggccgactt
     6361 cttcatcgcc aacctcatcc ccgtcaccct ggggaacatc gtcggcggcg ccctcttcgt
     6421 ggggacgatc tactggtata tttacgaccg ttagggtcga aagaagcttt gacggggcgg
     6481 ttttatgccg gaggctcccg gagcccccgg ccgcaaagcc cctccgaggg cctcagccac
     6541 agggcgggga gagcccccgg ggagcgatcc tttcctccaa cctcgggagg ttgagggaga
     6601 gaagagctga gtaaaatgtc tttacatcac gtccggacgc cgatgacgtt ctcgcagcag
     6661 tcgtcgccgg agcacatgca cttggtgtac ctaaaggtgc atcgggggtt gaggttctct
     6721 acggcggacc tgacgtactc ctggcaggtc gcggacagct ccccacaatc tgctcctatc
     6781 tccatcgcct ccttcaggag ggggcagccg gtgatcttca tcaccacctc cccatcggag
     6841 gagccggaga cgacccagga gaagttgggg ccgtagatca cgttcgatac gacgcagaag
     6901 acctcgcccg cctcagaatc tccatcccaa tctatcaaat ctcttgcctg cataatcacc
     6961 gcctcttaaa gttgttttgg agttggctta atatgtattt gtttgagatg ctttcacaat
     7021 ttttatgaaa ttttaagaat atatcataat ggccggggag gatcgaccgc caggacggcg
     7081 aagggcccaa agagacgata gccgaaagag attcatatca taacatcgac atattagtgg
     7141 agatgtagga tggaagtgat cgtcaagagg tcgccccggc ggaggaagac cctccaggcc
     7201 cggatggtgg agggcaccct ggtggtgatg gcgccggcgg ccatatcgga ccgggagctg
     7261 gccgagcacg tatcgcggtt cagggcgagg atggccctga agatggggga gagggacgac
     7321 gcccacctgg cgaggagggc cgatcacctg aaccggaagt acttcgacga cctcctccgc
     7381 tggaggggga tctcctactc cgaccggcag atgaagaggt ttgggagctg caccattgaa
     7441 gatgggacga tcaggatcag ctcccggctg aaagacgccc cccagtgggt cgaggactac
     7501 gtcgtcgtcc acgagctagc ccaccttctc gagccgagcc acaacaaaag gttccgagag
     7561 ctggtgggcc gctatcccct gacggagagg gcgatcggct ttctcttcgc cctcgactgg
     7621 atggagcggc gaaaggggag ggagggcctc actccagagc tatcatgacg ctcgtcgcct
     7681 tcaccaccac cgagacctcc tttccctccg agagccccag ctcctcggcg gacctcttcg
     7741 tgatcacgga gacgatctcg tccccgccgg agagctccac caccacctcg gcgctgacga
     7801 cgccgtaagt caccgacttc actctgccag agaggacgtt ccttgcgctt atctgcatct
     7861 cctttcacct ccttcaatta tctgatatcg aactttttat agatgccgat ctcactggcc
     7921 caggcggatc gatcccccct ccggtcaggg gtgggcggat ggtatcgaag aagaagaagc
     7981 atccaaaaaa gaggggcctc caagatggga gatgcgatcg tccccatcac ctctcgcctt
     8041 cccgggaggc tgtaccggcg agaccgaccc gagaggcgga aagggtttcg atgctcgcag
     8101 aaccggcccc gcatatgcgg cgactgccgg agcgggagag gaggagaggg gagaatcacc
     8161 gccctgccct cggctagccc acgagaatca ataccaggac ggctgtcccg tataccctag
     8221 tctccgggct tcatctattg ccaggatcgc atcgatatgg ttgaccgatt catagagggc
     8281 gaggcctttg ttataccagg ctcttgcata ctcaggatct aatctcgtgg cctcgtcgag
     8341 agcaatgacc gcctcgtcgt acttgccatg cttgtataga gcaacgcctt tgttattcca
     8401 agcggctgca tactcaggat cgatctcgat ggcttcatcg taagattgga tagcctcgtc
     8461 gtacttgccc tggttcctga gagcattccc tttgttatac caggtcatag caaactcagg
     8521 atcgagcccg atagctacat tgcaggcctg tatagcctcc tcataattgc ctagcttatc
     8581 aagggccact cctttgatta tccaagcatt ggtataatca ggatcaagct tgatagcctc
     8641 atcgaaagcc tgaattgcct cggtgtagtt gcccattaga taaagagacc tgcctttgtt
     8701 atatagggta taggcaaact caggatccag cctaatggcc tcatcgtatg cgcggatagc
     8761 ctcctcgtaa ttaccctgat aagagagagc tgttcctttg ttataccagg ccatgctgta
     8821 ctcgggattt agccttatga tctcatcgta tgcccgaatg gcttcgtcat acttggcctc
     8881 gtaaaagaac gcattgcctt catctagcca ttctttaaca gtctcttgtc cgcatacggt
     8941 catcgccagc aacgccagcg ccagaacgta caatatcagt tttagtctca tcttaatcgc
     9001 cctcctggaa aataggctgc atccaacctc gaaatatgta tcggtgtcat cgccccaaaa
     9061 tgaactccac cagccccgac aatatcctcg cctcttccgc cacattcgcc acgatctccc
     9121 cgaagtaaaa tccctcagct tggagatccc ccgccctcag gggaaatctg ttccaaagtc
     9181 tcaacgaggg gatgatactc gtccactact gattctcgcc aaaaatcaaa gtcggttctg
     9241 ttcgcttcat tagtagcatt ccccaactcg caaagaacac cccatgaatt cgaatatttc
     9301 tttaatttta atgttcgttc tatctctata gcccagactt tctgtcgtgt cctatcattc
     9361 tcggtaaact cgtttttagg tgcgcgtttt tcagactcga taaattctgg cacgtaatct
     9421 tcggatggta atactaagtc atttaatgca tctcgaatat ctgatcgccg ataatttgca
     9481 catttcccga cgtacctagc tcctccccgg aatggtggcc gtccaatcca ttttttcgct
     9541 agtagatcta agctgatgta ggacatggcc atgcagcatg attcaataaa aaaattactc
     9601 cgatataagg gtgtcgcgta tgatagacca ccaatataaa catgccccaa ctcatgagcg
     9661 aattgataga cgatctggca gaattcgccc gtattcaagc aagtgagatc tatatcatat
     9721 tgattctgca aaaccggggt tctaacacgc ggcaagccat cagggactaa atggaggatc
     9781 aaaggcttcg gacctagtgg cggattttct gcaaccttca acctatagat ttttataaca
     9841 tcattacaga ctgccatttc atggcgcatt agtgaattgt ttttgcagcc ttccatcgat
     9901 tgaacttcta gtttgatttc caccgccctc ctttgttgtg tgagactact gcgatcattg
     9961 aaatatattt cggctttagt catatgaacc taaaatatgc taatgttttt agtgtcacat
    10021 aactcaagga gatcaaagaa gccttaacct ttctgctttc ggagctttat tgttgccatc
    10081 gtacgattta tcttctcgac cgatcccagg tacacgtttt agaccttgct gccttctctc
    10141 caactcgcat acgagtaaga tcgaattcga ggggcacggc cctcttccgc cccctcccca
    10201 attctcactt cagatcgtac ttccgcccgg attgagggac gacgacctcg gcattgatga
    10261 cggagctgca aaatagagag cccgaaataa aaatagacag attctaatga gaatcaccag
    10321 gcatgatcgc ccatcgtcgt cttcggcacg atgagatgcc gtcgacatca agcggtgagg
    10381 ctgtgggatg cggacccggc ggcggccgta ctcgtcgtac ttcccctccc agaccagctc
    10441 cgtcctcatc gtggatatgg ggtggaggtt gtcggggccg aaagggaggg tggatgaaat
    10501 ggggtcaggc atggcggggg gcctccttac gatcgaggac gaagctggcg aggtccagcg
    10561 gcatcgagta gtgcagatgg gacaagttgg ccggaacaag tggtacggca agcggcagcg
    10621 ggattagagc tggattagga tcggacatgg tatctaccac ccaccttcta aagatccatt
    10681 acgccataag tatttccctc ggggtctgtt atggttatga agattatcgc cagatctgcc
    10741 gctctaaaaa ctctaaccct ccagaacgcc gcttcccgtc agtatgctct tgatgtaatc
    10801 gggagtgaac ctcttcttgg ccttgtggac ggcctccacc agtcgatcct cttctacatc
    10861 gaagttgtcc ttgaggtaaa gggcggtggc gatcagcgag agatcaacgg ctttgaatct
    10921 gccgaaccct gttactagat catcaactgc cctcttctct tcgccggtga gaagatgctc
    10981 gaactgcccc gctctctcgg tcggatttat ataatatcct tcggcatcct tccaggttat
    11041 atcgactatg ccatttctct cagcaaagtt cagctccccg gatacctctg aggaatacgg
    11101 gccgtaatga tacatggaga agctgaaatt tgcgacgtcg ctcctggtga gaaggtacat
    11161 catcttctga attatggtct tgcctatctg cttgccgggg tattcttcgt caagcctctt
    11221 gaccaggtat ccgatcaatc ccgagtttct gaagatgctc tcttcaggcc catcatttat
    11281 tctcatctca acgccacctc tgctttggct cggtcttgcg gccatacata gatccttatc
    11341 cgctttacat ctccaatatt cttaactatt gatgaataat atgacagggg gtgggacttt
    11401 cgagcttaaa tattaaatgc gcgcgcggga cgatagtatt attgagatcc catgccaggg
    11461 ggatcgagga tggcggggtt cgagtagacc tctcccaaag cccctcttcc gccccctccc
    11521 ccatcctcac ttcagatcgt actttccccc ggcttgcggg acgacgacct cggcgtcgaa
    11581 ctcgcttctt atcgcctcgg cgaaggagcg gctcgcctcc ggctccccgt ggttgacgaa
    11641 gacctgccgg ggaaactctt tgaaggagcg gagccaattg agcatacccg tccgccccgc
    11701 atgggccgag aaggcgtcga gatattcgat ccgggcccgg acgttccagt ccctgttcat
    11761 catcctgatc gacttggcgc cgtcgaggag catccggccg gtggtccctt ccgcctgata
    11821 gccgatgaag aggac
//
LOCUS       DASM01000089           10051 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_75966, whole genome shotgun sequence.
ACCESSION   DASM01000089 DASM01000000
VERSION     DASM01000089.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10051)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_75966"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1780 a         3316 c         3167 g         1786 t
ORIGIN      
        1 gggggtggcg gcccggcgcc ccgatccatc accagaattt ataaatcaac ggaaaaaccc
       61 tctcctgaag gggatgatag ccgcaaagcc aggattaatg gagaaagggt ccaacgccgt
      121 cggttatact attgggggac cttgatgaag gtccagcaag atcaaacgca taacccctac
      181 gatgccatcg tcgcgctctt tgactatcct ctgctgacag cgaaatcaga aattatctgc
      241 acaaaagatt tatttttttt cagtcgcgcc ccagtgcaaa ctnncacatt ccgcaccttg
      301 agttctgaaa ttggtatttt taccgctatc gatttataat ctcaaattct attattttaa
      361 ttcaatatga tattttaatt tgatttggat gacattattt tgattatcta attcaacacg
      421 actcgatgag atactcgctc accaggggtc agcagtaagc agacttaata ttaatataaa
      481 tgaatatcta tatttataat tatattggtg gaatcgcagg ctagcaaaat actcgattcg
      541 gggagcaaaa gtgcgaaatg cgggatttaa cttaaccata tttttaaact gtggggggac
      601 tggtgatggt ggcccgggaa actttctagt aaaataagat gagatccgcc cctacccttt
      661 ccggcagggg cgttggggtg gcggccggaa aggggcctca gccctccgcc ccgccttcac
      721 tcccggagcc ctccaggtag gccatcgcct cggcgagctc cgagagccgc ccctcatcct
      781 cggggagggg cgcccccgag aggcccctcc cctggatctc caggacctcc tccctcagat
      841 ccaggatcag gaggagggcc tttatctcct ccaggcggtc gtcgtccccc tcctcggccg
      901 cccccaggag ggccctcctc tcgatcccct tctcctccag ctggagggcg agcctctccg
      961 cctccaggga cagccgccgc ccctcgagct ccagccgcgc cctctccacc tccaggaggg
     1021 ccatctccgc ccccagaagc tccagcctct tcgccttcag ggctgccgcc tcctccgggg
     1081 tcagggtccc ggccgcctcc ttcgccagga ggggctccat ctccccagct atcctctcca
     1141 ggtcggaggc agcctccacc cccggggtcg ggaccccgtc cccttcgagg ccagctccgt
     1201 cgcctccatc tcctccgtcc ccatctccgg cgccatcgtc cccgtccccc tccccagagg
     1261 ggggtgcggg ccccacgggc tcctccccgg ggcccgagac gggcgcgggg gtgaaggtac
     1321 aagtcccccg gaagaaggcc cacgcctcac actcgctcgc gtcggggaag acgcagtagc
     1381 cgatctggtt tcccgtcacc gggtcggtcc tgatctcgtg ccggtatccc ttgtttacac
     1441 aaaaggtggc cgcagggttg gtcacgggcg tgacgggggg ggccgaagga accacgttcc
     1501 cggtgatcgt caccgatgcc gaggaggagg agacgaagag ccgtggctcc tcccagtagg
     1561 tcgcagaccc gatcgaggcc atggccagga tcatgagagc ccacgccgcc aagagagatg
     1621 atctgttcat catttcaccc gcctgatgga tagcttcgga gctgaagggg ccaaaaatta
     1681 atccccgata ttttcagcta cctcttcacg atcttttttc ctcatattcc attagatgtt
     1741 acagaattgc atgactttta aaaattgcgc tccccagcct ccggagagga ggggctccag
     1801 ccccctgaga tcagaggagg gggcgaggaa gagggggagg aggacggccc ggaaatggca
     1861 gaagaagatg atgaagatgc ccatcaaggg atgaagggaa cgaagagaga tggggataaa
     1921 agatgatcgg gttagaggcc cggccggagg gtggggctcg gggcggtgcg gctccggggg
     1981 acggctaggc cccctcccca tccccggaga agcggatgga tatcgccggg gcgtcgcctc
     2041 cggaagcggc gacgaggtcg ccgccgcaga gggagatcga ccgctccaga tacctcaact
     2101 catcccggcg gaagggatct cggatccccg gaccccggcc ggcgatcctc accaaggccc
     2161 cctccccctc ctggaaggcg ccgatggcga cctcctccgc cctccccgcc gcgagcctct
     2221 ccaggatgtc tatgacgagg tcggtcagcc gctcgccatc gacgaccgcc ggggggaggc
     2281 cctccgagcc ggcgaaggcg acggagaccc tctcggggag ggggacgtaa gagatccgcg
     2341 ccttcagggc ccggagccac tcctcctccg tctccgcctc caggatctga tccccggggc
     2401 ggggcctccg gatgaacccc tctatcgaac cccggaggat ggaggtcaga tcctccgccc
     2461 tcaggcactg agggggccga aagcccctgt aggtcaccat atagtcgttc agaagccgcc
     2521 cagccctcct caggaggtgg tcttcgaaga ggtccgcgac cccgccccca tcgaagatcc
     2581 cctccagctt tcctacgatc cggcccgcct cgtggactac ctcgatctcc aagagcccgc
     2641 ccccccggcg gctcgagagc cgatcgtgcc agcgggagaa gaggtcgtcg acggccccgg
     2701 agtccaccag cgcctcgatc tccctcgccg cctccccctc ccccaggtcc tccaggacgg
     2761 aggagacgag gatcagcctt ccggcgatga tggtgaagag ccgctccagc tcccggaggg
     2821 cgacggcgga gtactcggcc gcctccctcg cccacccccc gtcgatcaag aggggatccc
     2881 ccagggagag ggtgtactgg aggaggcggc agagggtctt tttcgccgcc ccggcttcca
     2941 tgggcctccc gtggcctgga cagcattgga ggacctcccc cccctccaga agccagaggg
     3001 ccctccggat cgacccgatc tggtcggccc gggaccaccc cgggatcccc gccaccccgg
     3061 ggttggaggc gaagaggagg tctccgagga agaggagctc cccggcccgg aggcagacgc
     3121 tgtcggggct gtggcccggg gtgtggtaca cctcgagggc gtcgccgccc cccagctcca
     3181 ccctctggcg gtggaggacg accccgtccc cgatcccgaa ggatgcggtc tcgtactcga
     3241 aggccgcgcc ccgcccaagc tcgataagcc tccggcccgg ggcggcgaga tccgccgaag
     3301 agaggagcct cacctcggga gacctggggg ccacctccct ccccaggagg caggagaggg
     3361 tcatcgccgg gtcccccctc tccagggaga cggctcccgc ctcctggacc gccaccgcca
     3421 ccccgccgaa ctccgccagt aggtcccctc gggagagctc gaaggagtgg tcgacgtgga
     3481 cgtgggtcag gtagacgagg accggcctcg ccttctcttc ccagagctcc ccgatcacct
     3541 ccaccagccg gtccatctga ccgtcgtcgc cccccggatc gatgaggcag atctggtccc
     3601 gggcggatag gacgtaagag ttggaggaga ccatatcgac cgtcctgatg tacgggaaga
     3661 cctccacccc ccgggtgccg cccgcctttt gaagaacttc tgactccgct gaagctccct
     3721 cctcgccccc cgcccccgga cctcggggaa cggaccccgg ggcggggtcg ctccggggtc
     3781 ccctatcgtc agggaggcgg agaggtttag gccgtcctta tttataaata gatttgagcc
     3841 cagaagagct ctgtaccgga tcgctccggg accggagggg gagagaaccc cgtccgaagg
     3901 tggtgggatg aagatgatgc tgtccaatag aagttcgggg gacgtggaga tcctctcccc
     3961 gggggggagg ctcgacgctc gatcctcgga ggagattcgg gtgaggatgg aggggctgat
     4021 cgacggcggc tgccggaaga tggtggcgaa cttatccggg gtggattaca tcagcagctc
     4081 ggggctccgg gtgatgctgg ggtccctgaa gaggctgagg aaggagggcg gcgacctcct
     4141 cctcgccggc ctccagcctt acgtccggga gaccttcgag atcgccgggt tcacccagct
     4201 cttcgagata tacgaccggg aagaggaggc ggtggaggag ttcgggcggc gggggaggcc
     4261 cccccgctag cccggccccc gccagccccg aggcgcggat ggatccgatc gtcagccccc
     4321 ccctcctcct cctcatcgag aagatctgcg tcatcgtcgt cgcagcctac ctcatcacca
     4381 ggacggagag cttcaccgag ctgatcgagg ggaggcttac gaggaagaac caggcggcta
     4441 tggccctgat cttcggcgcc ttctccatct tcgggacggt ctcgggggtc gaggtcgccg
     4501 gggtggtggc gaacgtccgg gacctggggc cggtgatggg agggctcgtc ggcggccccg
     4561 tcgtcggcct cggggcgggg ctgatcggcg gggggtaccg gctcctccag ggggggttca
     4621 caggccccgc ctgctccctc gccaccgtcc tggtggggtc cctggcgggg ctcgtctacc
     4681 tcgcaaaccg gcggaggttc gtgggggtgg ggggggcggt cgtcctcacc gccctcttcg
     4741 aggccctcca catgctcacc gtcctcctcc tcgccgagcc ccggccggag gcggtgaccg
     4801 ttgtccgggc ggcggccgcc cccatgatcg tctccaacgg cgtcggagcc ctgatcttcg
     4861 ccctgatgac gaccaacctg atccgcgagc gggagacggc aaaagagcgg gacgagtacc
     4921 tcctcgagct ggagaggagg aggctggagc tggagatcgc ccggaagatc caggagagct
     4981 tcctccccga gacggtcccg gccctgaggg gctttgaggt ggcggcgatc agccgccccg
     5041 ccgaggaggt ggggggcgac ttctacgact tcgtccccac cccggggggg aggatgggga
     5101 tcgccatcgc cgacgtctcg gggaaggggg tccccgccgc cctcttcatg gtcctctccc
     5161 gggcgatggt gagggctagc gcctccgggg agccgacggc gggggaggtg gcgaggaggg
     5221 cgaacgagct gatctccgcc gacgccaact ccgggatgtt cgtcaccctc ttcttcgccc
     5281 tcctcgacga ggaggcccgg accctcaggt acgtcaacgc cggccacaac ccccccctcc
     5341 tcctgagggg cgggaccggc gaggtcgccc tcctgaggac ccgggggatc gccatggggg
     5401 tcctggagga ggcggagatg gaggaggggg aggtcgagct ttcggagggg gacgtggtcg
     5461 tcctctacac cgacggggtg acggaggcga cggacgccgc cggggagcag ttcggggtcg
     5521 agaggctcct tctggcggcg aaggagcaca ggagcctccc ggcggcgtcc ctcgtcgagg
     5581 agatcgccgg ggctgtcctc gccttcagcg agggagagcc ccagttcgac gacatcaccc
     5641 tggtggtgct gaaggggggg aggagggggt gagggggagc ttcgagatgg tggcggagag
     5701 caggaccgaa agcctcgccg ggataagcga gttcatcctc tcctcgacgg aggggtcggc
     5761 cctcgaccgg cgggataggt tccgcctgat gatggccgtc gacgaggcgg cgacgaacat
     5821 catcatgtac agcggctcgg tggggatcaa gatcgaatgc cgggtctatc gcgaccgggc
     5881 cgtcgtcctc ctccgggacc ggggggtcgc cttcaacccc ctggaagccc cggcgccgga
     5941 ggtggacctc cccctggagg agcgggaggt gggggggctg gggatcttct tcgtccggaa
     6001 cctcaccgac ggggccgagt accggcggga gggggatgag aacctcctca ccctgacggt
     6061 ccgccaccgc gggggagggg cttaggctat ccctcccccg gagaggggcc cgatcccacc
     6121 gcgaccccag aaagcccctc atcgagaggg aggaccgaca acggtttaag tcaattatgc
     6181 agaagagtct ccaagaccat aaatggctaa gagaccgccg aacctcacct acgccgtcga
     6241 cgacgatccg cccctcccgg cgaccctcct cctcggactc cagcacgtct tcctcatcgc
     6301 cataagcctc atcttcccgg tggtgatagt gaggtccatc ggcgggagcc ccgaggaggc
     6361 ggagttcatg gtcagcatgt cgatgctggc ggcgggggtg gggacggtcc tccacgccct
     6421 caaccggcgg ggcgtcggct cggggtacct ctgccccgcc ctctgcggac catccttctt
     6481 atccgcctcc ctcctggcgg cgaacgtcgg aggcctccac ctcctcttcg ggatgacggc
     6541 ggtggccggc tccttcgagg tcctcttatc gaggttcctc caccggctcc gggtcctatt
     6601 tcctccggag gtgaccggcc tcgtggtggc gatggtgggg atcagcgtca tccccacggc
     6661 cctcgcgaac ttcatgggca ggggcggcgc cgacgccgcg accgagatcc ccgagctggc
     6721 ggtggccctc ctcaccctgg ggacgatggt ggggatcagc gtctggtccc gggggaggct
     6781 gaggctctac tcagtcctgg tggggatggc catgggctac ctcgcctcca tccaccttgg
     6841 gatcctcacc gccgaccacc tcgacctctt cgccgaggcg cccctcgccg ccgtacccca
     6901 cctcgactac ttcggctggt ccttcgacgt cgccctcctc ctccccttca tggtggcgat
     6961 aacctgctcc tccctcaaga cgatcgggga catcaccacc tgccagaaga tcaacgacgc
     7021 cgactggaag aggaccgaca tgaagaacgt cagccagggg atcctcgccg acggcctctc
     7081 cgccatcttc ggcggcatcg tggggacgat ggggcagtcc acctcctcca gcaacatcgg
     7141 cctctcgatc gggacggggg cgacgagccg gaggatcgcc ttcgccatcg gcgggatcct
     7201 cgtactcctg gccttcctcc ccaagctcgc catggtcttc gtggtgatgc cggagccggt
     7261 gatgggggcg accctgatat actccgtcag cttcatgatc gtcgccgggt tccagataat
     7321 aatgtcgagg atgctcgacg cccggaggac gtttttggtg gggatacccc tgatcatggg
     7381 cctctccgtc gacgccctcc ccggcctcta cgacggagtc ccccccatcc tcgcccccgt
     7441 cttcagctcc tccctctccc ttgccgcggt cctggtagtc ctcttaaacc tcctcttcag
     7501 gatcggggtc tcaaagcggg cccggctcgt cctccgcccc ggggtcgacg acgccgactc
     7561 gatcttcaac ttcctggaga ggcaggggtc ggcctggggg gcgaggaggg aggtgatata
     7621 ccgggcgata tccgccacca acgagctctt cgaggccgtc ggctccgcag ggctcgccga
     7681 gggggatatc gatctggagg tctccttcga cgagttcaac ctggacgtcg tcgccaggta
     7741 ccgcggcgag ccccccgtcc tgccgtcgag gaggcccgac gtggtggacc tgctggggga
     7801 ggggccctcg gccagccttg cggccttcat ggtgaccagg tacgcaaact ccgtccgggt
     7861 cgacaccaga gatggggcat gccgggtccg gctcaccttc gaccactgag gccgcatccc
     7921 aactcggcca ggcggtctcc ggcaccctaa aaaccgtccc cgcccattat ccagaaatta
     7981 cgaataaaag aggtcgatgg agggagaggt ggccatccaa ccccaccttc cccagatcca
     8041 acttaaatat ccagataggt cgcctccctc agggcctcca ggccatggga atcggcggtt
     8101 tccgtgatcc cggccccgga gtgaggggca tatagcagga acggttcttg tggctcggac
     8161 gaggtgatta tttcaatatt catcgtcatc tcctcgtcat cggccacgtc tttggaggtt
     8221 atgttaaatg tcccttccat cagcctctgg gtgatctcca cctggggaac tctgcagtat
     8281 ccggaatggc tgctgagggt tggcctggcg cagaaggtga tcttagtgcc gttgatataa
     8341 tactcgtgcc ctctgtcgca ggtgctgcat tcccctctat aataactcga tccatcaacg
     8401 ctaaaccggg ccgcttcaat ggtgcaattc ggaacgacca ggtggatctc gcagttcccg
     8461 atgaatggat atttcaagct gtgataattg ccataatata caattccatc catggcgtcc
     8521 tttcgctctt cagccgagac gttcgtcccc gtggctgcga taaaatcgat ggcatcgtac
     8581 agctcagtcc tcagatcttc catcgcatca aagtcaaact ccgcggtcct cgccaccaag
     8641 atgctgccgt ccccggatac gtaaacccaa tcgccggtcg attcgttgag cctgtaaaca
     8701 accagcgagt ccttctctgt aaccatctca tacttgatct ctgctccttg cgagaggaga
     8761 tccaagctca cgggaagacc catattgaca gcggctcccg cgttaaactc aggcagctcg
     8821 gcggtcacgg cggcgatgcc aaggagcatc atccccagga ctattattgc aggcctaatg
     8881 gctttcatca ctaacccacc ccgggcatgt acgggagaac cagagatcaa ctttgcgctt
     8941 aagtaatata gaatcaagta atagtgtcac ccgctggggg aggcgggatg aagatctgaa
     9001 ccccgcccgt ggcgatgtct cacctggagg catggccacc cgggcccatt ttggcctccc
     9061 tgagagacgg cccaggatcg tggcaccgaa gagggcggcc agaggaaaaa agatccggga
     9121 tcatgcgaag taaattggtg cgggggatgg gattcgaacc cacgaacctc tgcaggacag
     9181 ggtcctaagc cctgcgcctt tgaccgcttg gcaacccccg ctcttcagaa tatcatcttg
     9241 acgccgagat atcaccggtc catcctactt tgaggccctc ggagacctca gaccagaagc
     9301 ctctccagca ccgccttcac cgaatggagc cggttctcgc tctggtccag gatcgcggac
     9361 ttgggcccgt ccatcacctc gtcggtgatc tcctgccccc ggtgagccgg aaggcagtgg
     9421 aggacgatgg cgtcgtcctt cgcgtgccgg aggaggtcgg agttgacctg atacctccgg
     9481 aaggcctcga cccgcttcgc cgcctcggcc tcgtcgccca tggagaccca ggtgtcggta
     9541 ctgacgacgt cggcacccct gaccgcctcg agggggtcgg cgacgacgac ggttcttcca
     9601 ccaagactct cggcctgatc taatattttg cggtctggct cgtaccccgg cggggcggct
     9661 accgccacct ccatccccat catcgccgct gcgaggatcg tcgagttgca gacgttgttt
     9721 ccgtccccga cccaggcgac cttcaagccg gcgaggcggc cgaaccgctc cctcaccgtc
     9781 atcaggtccg ccaggatctg gcaagggtgc tcgaggtcgg agagggcgtt tatgaccggc
     9841 acctccgcgt agcggcccag ctcctcgatg gttctgtggt cgtagacccg ggccgcaatg
     9901 gcgtggacga agcgagagag gacccgggag gtgtcggcta tcgtctcacc ccggccgagc
     9961 tggaggtcct tggtggagag gtagagggcg tgccccccca ggtcggccat cgccacctcc
    10021 agggagaccc tcgtcctggt ggagggcttc t
//
LOCUS       DASM01000090           12801 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_61966, whole genome shotgun sequence.
ACCESSION   DASM01000090 DASM01000000
VERSION     DASM01000090.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12801)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12801)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12801
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_61966"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2549 a         3914 c         4163 g         2173 t
ORIGIN      
        1 catcggtcct ggtgacgatc ccgaccaact tcttcggatc gtccctctcg accacgggaa
       61 gccgtccgac gttgtttctg tagaggcggt ccatcacatt cttcatattc tcgtcgggaa
      121 aggcgactat aacatccttc accgccacct ccccgaccct gacctcgtcc tgctttccct
      181 cgggaacccg ccggaggtcg tcaaaggtga cgatcccggc tagcctccct ccttcaacga
      241 cgggaaaacc ccggacgttc tcctcgaaga tcctcgccat agcctcccgg accgtcgtct
      301 ccggccccaa ggcgacgacc ttccaggcca tggcgtccct cacgaggacc cgctccagga
      361 gggcctcccc gccgtccagg gtgacgcccc tcctcaagag ctggaaggtg aagatcgtcg
      421 accccgatag gccgatggag gcgacggcat agctggcggc ggaggtgatc atgagggccg
      481 ggaggaggga gtaatcggcg gccatctctg agatcagtac cacggagctg aggggagccc
      541 ccgccgaccc cgccaggagg gcggcggccc ccaggagggc gtaggcggga gcgtgccccc
      601 cggcggcggg gacgaggtgg ccgaagagaa agccgaagga ggctccggcc acggcaccta
      661 tgaccagggt cggtccgatg gttccgccgc agccccccga gccgagggtc gaggcggtgg
      721 cgagggcctt cgccgccgcc agggcgagga ggaataggac cagacgggag ccgtccgaac
      781 cggcagggtc cgatgcgatt gcgagaattc tatcaacgcc gtcgtatccg acccccatca
      841 ccccgtaacc gatgaagtag acccctgccg ccccggcgac gagacctccc agcccaggct
      901 tcagcccctc tttcagcggg agccataaaa agagcctctc cgccctggag aggagccccg
      961 cccagaggat ggccagaccg ccgaagatca gccccataag aaagcagagc agaagctccg
     1021 gaagggtgat ctcggtccag acgggggtga aggcggggct ctggccgagg aaggcggagg
     1081 cggtcgcagc ccccaccacc gccgagagga ggatggggac ggcgtccacc atccgaaaac
     1141 gcctcataac cacctccatc ccgaagatgg cgctccccag gggggcgttg aaggtcccgg
     1201 cgagccccgc cacaaagccc gacacggcca ggatctgaac gtcggcgacg tccagcctga
     1261 gcctctggcc caggagggac ccgaaggagg ccccgatctg ggctatcggc gactccctgc
     1321 cggcgctccc accgctaccg atggtgatgg cggaggcgat gaggatggcg gggccggtcc
     1381 tgcggcggac cctccctccc cgcttatgta gggcctccat cagggccggg atcccctctc
     1441 ctctggcctc gggagcgagc ctcaggatca gaaggccgac ggcgagccct cccaggaccg
     1501 gaaggagtgc catccccccg ccgggaagga gggtcgcgac cggcccgaag aatagcccgt
     1561 ggacctctcc cgccagccac cggaagacta cggctccaag ccccccgagg agccccgtcc
     1621 ctacggcgag ggcgtccagg accagccacc ggttctctcc ccaggaccga atcatccagg
     1681 atctcaaagt atcctttctc aaaacgtgag caggatataa agacgtactg tctaagtcca
     1741 aatgtatgtg ccgaaatata tgaggagggt ccgccccgaa ggggttcggt tcgcacctcg
     1801 gagatccagt cgtttgtgag gctctggcca gccccccatc agctttccga accctgatat
     1861 aaaatgccct cctcctctac cacccagctg atgaggtccc gatgttcgac tacgccgata
     1921 ggtacggcaa aaggttcgtc acgctcatag acagccgctc caccggaagg gagtttgagt
     1981 tcgaccagat cctcctgatg ccgacggccc ggggctatcg ggccctggtg gagctggcaa
     2041 aagaggaggt cgacccgcgg gtgatgagga ttttgtcgaa ggtctggccc ccttcaacta
     2101 tagagaacct ctccttcttc gccatggccg ccgccgtcta cgcccggtcg gtggcggagc
     2161 cgccggccag gatagaggtc ggaagggtcc tcctccggac cgccgccttc tgccttcttc
     2221 ggtgcgcccg ccggaaggag acgggcggcg gctgcatggc ggcgaagggg ctcggccgcc
     2281 atcaggtacc caagtgcgtc aagctcccca gggacgtcga ggccgcttgg cggagcctga
     2341 agaggtacat cgccgccggc ggatcggtgg tggaggggga gaggacctcg ggggcgatga
     2401 aggtcgactt cacgctgcct ggcgactact atcccgccac ctcctgctga tccccccgca
     2461 cctatgcctt cctccaactc cgcctcccgg ccgggaccgt cccatccccg gccgcaccgc
     2521 ggggggggtc gcgaaggagg gggggcgagg ggggatatcg ccccccatct ccggagattt
     2581 cgatcctaaa tccggaacgg gaccggactg ccgccccatt tcagagaagg catttatatc
     2641 aagtccgcgc caaagagccg ctgactttca aggtgatctt ccatggccaa gattagagca
     2701 ggcgttctgg gtgcgacggg agcggtagga cagcgtttca tcgagcagct ctgggaccat
     2761 ccctggtttg agctcacaag cctcgccgcc tccgagagga gcgcgggaaa gaagtacagc
     2821 gaggcggccc gctggcggct ggagcacgag cttcccgcgg atgtggccga tatgaccgtc
     2881 gtcaacgtcg acccccggga ggtcaaggcc gacgtcgtct tctcggcgct ccccgccgac
     2941 gaggcgaaga aggcggaggc cgagtttgcg aaagcggggt tcgccgtggc gagcaacgcc
     3001 agcgcctacc gggcagagcc ggacgtcccc ctgatgatcc ccgagtgcaa cgccgaccac
     3061 ctcggcctcg tcgaggtcca gcagaggaag cggggctggg atggctacat cgtcacaaac
     3121 cccaactgca ccaccatcat gttcgccctg accctcaagc ccctgatgcc gatggagatc
     3181 cagcgggtga tcgtcgcctc gatgcaggcg gtcagcggcg ccgggtacga gggggtcccc
     3241 tcgatggcta ttctggggaa catcatcccc ttcatctccg gcgaagagga gaaggtggag
     3301 cgggaggccc agaagatcct ggggaccttc gacggcgacc ggatcgtcga cgcccccttc
     3361 gacgtcagcg cgagctgcca tcgggtcccc gtcatggacg gccacaccga ggcgatctgg
     3421 gcggagatgg ccgccgaccc cacccctaaa gaggtgaagg aggcgttcct aaacttcaat
     3481 cccggcctcg gcgaccttcc cacggagccg aagaggccga tcatcgtccg ggacgagcct
     3541 gacaggcccc agccgaggct cgacaggaac gccgggaagg ggatggccgt ctccgtcggc
     3601 cggatcaggg agggaatccg ctacatcgtc atgggccaca acaccgtccg gggcgccgcc
     3661 ggcgcctccg tcctgaacgc agagatcctc tccaagaagg gaatgcttta gatgctgatc
     3721 atagcctggg aggtgacggc cgcctgtaac ctggcttgcg agtactgccg cgcctcagcc
     3781 tcggcctcgc ccgacggggc cgagctctcg acggaggagg cggaggggtt catcgaccag
     3841 gtggcacccc tccggccgat gctgatcctc agcggcgggg agcccctcct ccgccccgac
     3901 atcttccacc tcgccgaata cgcccggagc cgggggatcc gggtatccct agctacgaac
     3961 ggaactctcc tcacccccga ggccgtcgag aggatcaaac gggccggtat catgcgggtc
     4021 agcatcagcc tcgatgggcc cggccccgag gtccacgacg cctcccgcgg tccgggctcc
     4081 ttcgaccttg cggtccgggg gataggaaac ctccggggcg aggtggactt ccagatcaac
     4141 atgacagtga cgagggggaa ccaaaacgag gtctccgcca ccatggacct cgccgaggag
     4201 ctcggagccg tcgccttcca cctcttcttt ttagttccca cgggccgggg gagggaggag
     4261 gatctggtga cgccagagga gcaggatgcg atcctccgag aggtggccgg ggaggggagg
     4321 aggaggaaga tcgaggtgaa ggtcacctgc gccccccagt acggccgggt ggtgagggag
     4381 gtcctcaccg aggcggaaag ggagggaatc atggggagcg cctgcctcgc cgggacgaac
     4441 ttcgtcttcg tctcccggac cggagacgtc tctccctgcg gctacctccc gatagttgcc
     4501 ggaaacgtcc gggaggagag cttcccggag atctgggaga gctcgccggt cttcctggac
     4561 ctccggagga gggagctgtt gggcaggtgc ggcggatgct cataccggag ggtctgcggc
     4621 ggctgcaggg cccgggccta cgccgccacc ggggactatc tcgagtcaga ccccctctgc
     4681 agctacgatt cggccgaaga ggatctgaag gaggacggcc ggaccgaggg ggcgcctccg
     4741 gggagggagg ggcccgacct cgacgagctg gaccggaggc tcctggagat agtccaggag
     4801 gagttccccc tgacggcccg gcccttcctc gccctcggcg aggccctggg gacgagcgag
     4861 gaggaggcga tggagagggc gaagcggctc caggactggg ggataatccg gaggattggc
     4921 ccgatcctgg acatgaggag gctcggctgc tccggggtcc tcgtcgccct ccccgtctcc
     4981 gacgatagga cggaggaggt ggcggaggtg gtcaacagct accaggagat ctcccacaac
     5041 tacctccggc cgaacgagac ggagttcaac atgtggttca ccatatccgc ctccgaggag
     5101 aggatcgagg agatcctctc ggagatgaag gagaagaccg gcctccacca gctcgtcctc
     5161 ccgacgaagc ggatattcaa gataggggtg cgctttgaga tcccaagatg agagagcggc
     5221 cttccccgag gtcgtcctca cggagtttta caccgaagcc tgcggcttct gccgcgagga
     5281 ggccatgatc ctggaggagc tggcggaggt cttcgctcgg aaggtcgagt tcaggaggat
     5341 cgacgcccag gtggagggga ggctcgccga ggagcaggtc gtcttcaacg tccccaccct
     5401 gatcctcgag agggacgggg agttggtgga gaggttcaat ggctttgtcg ctggcgagag
     5461 gctggaaagg gccataagag atgctctgag gtgaggaccg gaaggcttcg gcctcaccgc
     5521 ctcataaaaa attttccggg gatgggggcc gttctgatct accgggtctt tcgcccctca
     5581 gccccccttc tttcccctac tccaacgacc ctgtccttgc tatcgggatc tatccatctg
     5641 gtgctcaggg ggacgggtca gcttcctcct cgctggactc gaaataatca cacaggtcga
     5701 tctcttcaaa ctcgaagacg atggggctgc aggtccctgt agctatcaac ttcagggtgc
     5761 agtcgaagac cgcgccctcg tttatctgat acttctcaag gaaacgaggg ccggggtacc
     5821 aggagttgca gagctgtaag agatgctcgc tgcccaggat ctcctcttct cgatccgcac
     5881 ccaggttcat cggctcgtcc gggctgaaga cgaaccagac ctcatacccc tcataaccgg
     5941 ggccgccctc gacctctgcc tgtcggatcg aatcctcggt cttgaggatg gagacgatct
     6001 cgcaagcgcc cgggtagtca tcgtaagagc accctccgcc gatcagaccc tgcaccaatg
     6061 ccgtggaccc gcagaggaag agcagagaca acagagtcac cgatgccttc ttcttcataa
     6121 cctttcctcc agctgtgctt tagtcctttt atctcaaaca tgtcagataa aacttctccc
     6181 tgtctccgta gaatcaaggc tcttctgcat cgggatcgac cctccccggg gatcctctgt
     6241 aagagatccg atagacagct cccgccctgt cgtcggagac gaggagggcg ccgtcgggca
     6301 tcaccagaac gtccacgggc cgaccccagg cgacccctcc tccgagccac ccctcggcga
     6361 agatctcgta gtcggtggcc cgtccgtcct cgaacctcac aaaggtcacc cggtatccga
     6421 tggggtcgac ccggttccag gagccgtgct cggcgatgaa gatcccgccc cgatacgccg
     6481 gcgggaaggc ggcgcccgag tagaacctca tcccgagagc agcgacgtgg ggccccaggt
     6541 tcatctccgg aggcgtgaac tcgccgcatc ccctcgaccc gccgaagacg ggatctggga
     6601 ttccggcatg gcagaaggga aagccgaagt gctggccggc gacggcggcc cggttcagct
     6661 cgtcgggggg ctcgtcgtct ccgagccagt cgcggccgtt gtcggnnggt gccagtcgaa
     6721 gccgacggtg tttctgactc cccgggcgaa gatctcgaag tttgtgccgt cggggtcgat
     6781 cctggtgatg gaggcgtagg ggtcgcccgg atcacagacg ttgcaggggg ctcccatggg
     6841 gacgtagagc ttcccgtcgg ggccgaaggc gatgaacttc cagccgtggc ttctctggct
     6901 gggaagatcg tccttgacca cgacgggatc gggcggttca tcgaggtgcg actcgatcgc
     6961 gtcgtacctg aggatccggc tcacctcggc gacgtaaagg ctcccatctc gaaaggcgac
     7021 gccgttcgga aggtcgagcc ccgaaccgat gacgaagatc tcgccctctt cgatccggcc
     7081 gttcccgtcg ctgtccctga tggcgtagac cctcccctca gacctcgtcc ctacgaagac
     7141 cgtcccctcg cttcccaggg cgagggaccg agcgccggga aggtcgcgag cgtagacggt
     7201 gatctcaaag ccggggggga gctcgatcag atcgatcggg aggtcctggc cgctcccctg
     7261 gggcgagttc aaaaagacga cggccaggag gagggcgggg aggaggcctt tgggcttctt
     7321 cttcatcatg agggtcgcca ccatcaattc ttcttagggg taatatcccg accttctaag
     7381 ctacaaaatt ttcgggccgg gatcatcccc cgccgaagag acggattttg gggagtcatc
     7441 cgagagggat gagggagcga gggcgagatg gaggctcctg gaggagaggc gtggagcatt
     7501 ataagggctc tgggatgaag catcagcgag gtggatagcc ccgacccctc ctaatccaag
     7561 atccagggcc gaggagaata cttaactaga aactcgtcca taaaaagcca tctagagggt
     7621 gtaaaagaga tgaccgactc cgaatccatc cggctgaaga tcattgaggc tgaccagagg
     7681 gacgtgggaa aggggatcgt gaggataagc gggcggcaga tggccgacct gggggtcgcc
     7741 gactacgacc tggtggagat cagtgggacg aaggctacaa gcgccctcgc ggtgaaggcc
     7801 taccccaccg acgaggatat ggacgtcgcc cgggtggacg gcctcataag gtcgaacgtg
     7861 ggggcgggga tcggccagta cgtcgagatc tccaaggccg aatggaggcc ggccgagagg
     7921 gtctctttag cgccggtggg gcgcggaatc cagatctcga tccctagcga ggccctccgg
     7981 aaggtattct tgggacggcc cgtctcgaag ggggacgtca tctccaccac caccctccgg
     8041 cggccgcccg gcgacctcgc caccggaaag gacaccatgt tcgacgagat cttcaaaagg
     8101 tctgataagg gctccgcctt cggcctcggg gaggtgaaga tgagggtcgt ctcgacggtg
     8161 ccatcgggga cggtcaggat cggcgaggag acggagctgg agctcctctc ccgggcggtg
     8221 gacgccaagg ccgcggcgga ggtggtctac gaggacctgg gggggatgaa gcacgccatc
     8281 cagcgggtcc gggagatgat cgagctcccc ctcaagcatc ccgagctctt cgagcggctg
     8341 ggtatcgacc ccccgagggg ggtcctcctc cacggtcccc cggggacggg aaagacgatg
     8401 ctcgccaagg ccgtcgccaa cgagtccagc gcccacttcg cctcgatcaa cggccccgag
     8461 atcgtatcca agtactacgg cgagagcgag aagaggatcc gggaggtctt cgaggagtcg
     8521 gagaggaacg ctccggcgat catcttcctc gacgagctcg actccatcgc ccccaagcgg
     8581 gaggaggtgg cgggcgagat ggagcggagg atggtcgccc agctcctctc cctgatggat
     8641 ggccagaagg agagggcgaa cgtcatcgtc atcggggcta cgaacaggcc cgacgccgtc
     8701 gaccccgccc tccggaggcc cggcaggttc gaccgggaga tagagctagg ggtccccgac
     8761 ttcgaagggc ggcgcgagat cctccagatc cacacccggg ggatgcccct cgcccaggac
     8821 gtcgacctgg aggagttcgc caccctcacc tacggcttcg tcggggctga catcgccgcc
     8881 ttctcccggg aggcggcgat gaacgccctc cggcgggtcc tccctaaaat agatctcgac
     8941 gagcccacca taccccggga ggtcctggag gagctggtgg tgaccagggg ggacctggag
     9001 gcggcgatgc acgaggtctc tccgagcgcg ctgcgggaga tcctgatcga ggtgccgaac
     9061 gtcacctggg ccgacgtcgg cggccttgaa ggggtgaagc agctcctcgt cgaggcggtg
     9121 gagtggcccc tggtctacgg cgagaacttc aggaggctcg ggatcgaggc gccgaaaggg
     9181 gtcctcctct acggcccgcc ggggacggga aagacccttc tagcgaaggc cgtggccaac
     9241 gagtcgaacg ccaacttcct gacgacgaag gggagcgaga tcctctcgaa gtggtacgga
     9301 gagagcgagc ggcatatcgc cgagatcttc agaaaggcgc ggcaggtggc ccctgccatc
     9361 gtattcctcg acgagctcga ctccctcgcc cccgtccggg gcggagggac cggcgagccc
     9421 cacgtcaccg agaggatagt aaaccagctc ctctccgaga tcgacgggat ggaggagctg
     9481 cggggcgtcg tcgtgatagc cgccaccaac cgccccgaca tcatcgaccc cgccctaatc
     9541 aggcccggca ggttcgacga gctgatcatg gtgccggtgc cggacgccgc ctccaggagg
     9601 aagatcttcg ccgtccacac cgggaagatg cccctggccg aggacgtcga cctcgatcgg
     9661 ctcgtcgaga ggaccgacca gtacacggga gccgacatcg cctccatctg ccggaaggcg
     9721 gggaggctcg ccctccggga ggatatgaac gccgtcgagg tgagaaagag ccactttatc
     9781 gccgccctgg aggaggtggg accctccgtc acccccgaca ccatgaagta ctacgccaag
     9841 ctctcggggg agctgcggaa gaagggttcc cgggccgtcg agaaagcagt ggaggagatg
     9901 gagaaaaagt gaggagaaga gcgggacggg ttgcatctcc ggatgggggt ggcggtcccc
     9961 gctccggaga tcggacctct tcagcccttc agtcctttgc gatcctcctt cctaagaggt
    10021 acaacacacc tccaccgagg acgacgccta tgacggcaga tagtatataa gctatgaccg
    10081 ctccccggct cgactcgtcc ccctcaaagg cgtagtctgg gagaggggcc ctctcccaga
    10141 aggtggagag ctcctttagc ccctggggca cataccccag cttctcttct atctcttcgt
    10201 tcccccactc cccgaaggtc tctccggtgg cgaggagccc cagggggacg aggaccacga
    10261 ggagcaacag gcctatgacc aggtttctta tcgacttttc catggtcgtt cacctcaggc
    10321 cgcagcaccc ttcgccagcc tcttggaggt cgggcggtcg tacatgagga gggacgggtc
    10381 ggtcttctgg atgtagacga agatcagggc ggtgaccgcc gcctccacga agctgaagaa
    10441 gaatacgtgt tcagcgagca tcgccggaag ggccacgttg aggccgtagg gcatatagag
    10501 ggggatgccc gccgcgtcgg tgtggagaag cggctggatc ccgaacatga agcctgtgag
    10561 gcctgctgcc agggtgaggg agacgtatcc agcgatcgcc gaggcagcta cccttttatt
    10621 ggaggtgatc tcggagcttc cgcttatgat cttatagacg tagaaggcgg caaagggcat
    10681 caccaccgcc atgttgaagc agtttgcggc tattgcggtg acgccgccat ctccgaagat
    10741 cagagcctgg aggatcagag cgacggatat ggcgataacc ccggcccagg ggcccaagac
    10801 tatcccgatt atggcgccgc cgacggcgtg gccggtgctc ccgcctggga tcggcatgtt
    10861 gaacatcatt atgacaaagg agaaggccgc agcaagagcc agaagcggca cctggcgggt
    10921 cctaagctct ctgctgagcc tctgcccagc gtatagccat atggggatca tgatgatcca
    10981 catggcgata taggtgtatg gccctagata gccatctggt atgtgcataa catcaccgca
    11041 gattatttga ctatcatggg tataaatatc tatctaaatc aacacatagt cagatgatat
    11101 gattttataa tattaattta tctatctgga taagatttct tatacaaaaa gtcaaaaaag
    11161 gaagatcgta gagatttaga ttgatggatc agtttccact cactttttat aactggtatg
    11221 ctgtcgatct gatattcctc cgacaaaata tcagatcgac accgcaggat catcggtcac
    11281 gccttccgcc cctttggatg gaggtgatga tgcgggtggt aatgctcttg gtatacggtc
    11341 ccatggcggt gcatgtgttt gtggatgagg tttgccgcca ggaggaggtc caggttccct
    11401 attacgtcgc aggaccggcc gtcggctttg atggtgtggt cctcgccgat gaccaccgcc
    11461 ctcttgctga tgtgctcgac ggtgtccagg tcatgggtgg ccacgacaat agtctttccc
    11521 gccctgctca gctcctgaaa gagctctatc agccagagct gagtcctggg atcgaggccc
    11581 gccgtcggct cgtccaggag gaggacgtcg gggttcgatg ccagagccga tgctatgcag
    11641 accttcttct tctctccacc gctgagggtg tggggcgccc gactggcgag cttcgagatc
    11701 tcaaccatat tcatgacctc ttcgaccctc tccttcacct cttcctgggc catccccagc
    11761 tggagggggc cgaaggcgat ctcgtcgtag accgtcgagc tgaagagctg aacgtcggag
    11821 ttctggaaga cgaaggccac ccgctctcta tagaaggttc gaaactcgct atcggcgagg
    11881 gagtcgaaga cctcctcctc gacgagcctt ccgaaggcga agaactcccc ggaggtgggg
    11941 aataggagag cgtccatgat agataggagg gtcgacttcc ccgagccgtt ggtccctatc
    12001 aaggagacct gctctccctg ggatatccgg aggtttatgc ccaacagggc ctcaaacttt
    12061 ccgaggtagg agtatgaaac gtcctggaga tcgaatataa tcgacattat ataaacctca
    12121 tatttggacg acgttgaggg agaaagtcaa gagggcaaag gtgagggcga gggcgaaccc
    12181 cgcagcgaag taatcgcgcc attgaaggtg gtagtcgtgg aggatccgga cgtcgccgga
    12241 atagcccctg gagaccatcg ccatgtggac cttctcactc atgtccaggg actttattag
    12301 catgtagcct atcctccccc cgacccacct ctgctcctct cggaggctca tgctccttat
    12361 agtcctgctc ttcttcgcca agtggaggtc ccgtatcatc tccatgaaga ggaagatgta
    12421 tcggtaggcc atggccatgg tgaggacgta gaccttgggt acccggaggg atcggaggga
    12481 cttgaagagg agctgctggg gggtcgaaag aaataggagg actacggcgg atacggccgt
    12541 cgccaccctg agggtgaagg tggcggcggc gtcgacgccc tgctcagtta tgtagatcga
    12601 gcccggaagg gagaacggcc ccagatgggc atccgggggg aggtctatta tatggaataa
    12661 agggtcgccg gggaggaata cgttgagggt gacggggatg acgatcaccc ctgcgaagaa
    12721 gggtacgaag agccagaccc tctttatgaa gaagaatatc cctatcttgc tgaggcgggc
    12781 gaagaggagg gtgaggaggt a
//
LOCUS       DASM01000091            7365 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_61965, whole genome shotgun sequence.
ACCESSION   DASM01000091 DASM01000000
VERSION     DASM01000091.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_61965"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1438 a         2215 c         2262 g         1450 t
ORIGIN      
        1 agatcctccg gccgtcccag gtctttacgg tggtgtgcct cagggtcaga tcctctatct
       61 ggccgtactc gcccttgaag tcgacgaagt ccccgacgcg gaagggatgg aatatcgcga
      121 ggaagatccc ggatatcagg ttggagaggg aatcctgggc cgcaaagcct atggcgaggc
      181 ccgccacccc cgccccggcg aggagcgtca cggagatact gctaagggtc ggtatctggt
      241 atatcaccat cagaaacccg gcgacgtaga tcgccgccac catcagcctg ctgatcaggg
      301 tcctgaaggt tgtctcggtc tcggcgtcga ggccgatctt cccacccccc gtgatcttgg
      361 ggaggtggat gttgatcgcc cgtcggacta tattgacaag taaatatgta ataattatta
      421 taattaatac ttttaagatt attcccgcta tggccaccag gtcgatcccc agggcagagt
      481 cgaggtagtc gatgatcgat tcgatcttag aataggcgcc gtcccccgtc agctgggaag
      541 ggacctccga agatagctcc ctcggcagat ccgtggtgac gtttgtgagg ttcgaatcct
      601 ggtcgagacc gtccgggccc atcctggagg ggacggtata atcgtagaag atctcctcct
      661 ccccggatac gatctcgtca tcttccatga gacagaggaa aaatcggaaa tacttaacga
      721 tttcgctgat ggctcaagga tcgatcgccc aggaagaaga ggccgcggtc gtggcacccc
      781 gaccgcctcc cgggggctga aaaggcttta aaggaataaa acccgacgat aggcggggat
      841 ggtcccatgc caagaaaggg attttacggc ggcctgattc tgttctcggc cctgctcctc
      901 atcctatcgg ggggcccggc atcggcggcg acttacgtcg tcagcccgga ttacgacgac
      961 aagttcatcc aggccacaat aaaccgcgcc agcgaagggg acaggatcat cgtcaagagt
     1021 ggcaattacc gggagaactt aaacgtcacc aagaagctct ccctcatcgg cctggattcg
     1081 gggggaggaa agccggtgat cgatggaggg gggtttggga gcgccgtcgt catctccgcc
     1141 gacgaggttc tatttgaggg cttcgccgtt acgaactcgg ggaagctctg gaaggacgcc
     1201 ggaataaagg cgatctcaaa ggagaacacc atccggaata acatggtcac cgggaacgag
     1261 tacggcatag tcctcgacgg cgcagcccga aatagcgtcc tgaagaactt tgtctgcaaa
     1321 aacgacgtcg gcatctccct ctattcctcg gagcggtgca ccatcgtgga gaacgaggcc
     1381 tgcaacaaca ccttcggcgg catccttctc agcagggcca acaacaacac cataaggaac
     1441 aacaacgcca gccaaaacag gtgggctggg ataatcctcg gcgagtgcag cgacaacacc
     1501 gtctccaaca acgtcgtccg gcagaacgac aatcagggga tatggctcct ccgctcctcc
     1561 gccaacacca tccgaggaaa cagggtgagg ttcaactaca tcttcggggt ccgtctccta
     1621 tactccagca gaaacgccat caccagcaac acgataagaa ataacctcga cggcgtcagc
     1681 ctggagagct ccaacgggaa cgtcctcgcc ggaaacaacc tgagcatcaa cgagtacggg
     1741 gtctacgtcg acaactcctt caacaataga atttatatga acaacctcat cggcaacaat
     1801 aacgacgccc attcctggaa ctccaccaac tactggaact ccacagagct tttgaagtat
     1861 atttacaacg gcgttcagta caggaggtac gtcggcaact actggagcgg ctacttcgga
     1921 cccgacccct tcggcgacgg cctcgtggac aggcctcacg ccgtatcggg aagggagagg
     1981 gactacaacc cccttaagaa accctcggag aattacaccc ttcggggctg aagagatggt
     2041 gaaggaaaaa tgagggagga tgagggaggg ccccaagccc ggtcctcctc cccttaagag
     2101 aacatcctca tcgactcgtc gacccggggg agcatctcct cgccgaggac cttgtcgacg
     2161 gccctcttga agtcctccgg ggtgacggcg gttccccgcc gccttatggc catcatcccc
     2221 gcctcgacgg cgatggactt gaggtcggca ccgctcgccc cctccgtctc cttggctatc
     2281 actcccaggt ccacctcagg gctcatcttc atcttcgcag tgtggatcga aaggatggcc
     2341 aggcggccgt cgaagttggg catggggatc tcgatgatcc gatcgaacct cccgggccgg
     2401 aggagggcgg ggtccaggat gtctatcctg ttggtggcgg cgatgacctt gacgtccccc
     2461 ctcgccctga agccgtccag ctccgccaac agctgcatca tcgtccggtt cacctcggcg
     2521 gacccggtgg tcccatcgtg ggtcctgatg gatccgacgg agtctatctc gtcgatgaag
     2581 acgatcgctg gggccttgtc cctcgccatc tggaatatgt ccctgacgag ctgggctccc
     2641 tcgcctatga acttgtggac gagctcgctc ccggacatgt ggatgaaggt ggctcgggag
     2701 tggtgggcca ccgccttggc gatgagggtc ttgccggtcc cagggggtcc gaagaggagg
     2761 acccccttgg gcggctccac cccgatctcg gcaaagagct cgggctgggt gagggggagc
     2821 tcgacggtct cgctcacctc ccggatctgg tcctccaggc cccccacctg atcgtaagag
     2881 acggtgggtg cctctatcag ctccatcgtc ttggccctga cgtccacgga cttgtccatc
     2941 accctcacta tcgtcaggga gttgtttatc gcgaccctgg tgttcggctc cagagcctcc
     3001 atcatctccg gggaggtggt ggtgatgaac tcctggttgt taccgtgctg tcggaggagg
     3061 acgtagtcct cccccatctc caccaccgtc gccaggaaga ggggcatcct ccggaggagg
     3121 gcgttctctt tcttcagccg ctcctcttcc cgcatgtact tcttgttgga tatgagggtc
     3181 tctacgagct tggctctggt gtcctctttc tccatcctga gggtctcgat ctcctcactc
     3241 aggcgcttgt tctcgttctc cagagctgtg atcctcttta gatgtaattc ctgaatcgac
     3301 tcctgatctg gcgtaattgt tgactcagtc atgtaaatag tcgaatgctc attcaggaat
     3361 ataaattgca ccctcaggaa tagggccgat attataaatg accaaccccg cccggcggcg
     3421 gtcttcgccg gataatttac gccgcgggag agggcgccgc cgtaattttc ggaagaggat
     3481 caccgacgat ttgccaggta aaccacgtca ataagcgtac ttcatcatcg aagctccggg
     3541 gcaaccgatc ggaatatcaa gctggaggtt ctgtaatata ttcgggtgtt ttgatgatcg
     3601 agtttgagaa gtacagacct ttgaccttcg aggtggtgga gcggaagggg ctgggccatc
     3661 ccgacaccct cgcggatggg atatccgagg ccatctcgat ggagctatcg cgggcctacc
     3721 tggaggagtt cggggagatc ctccaccaca acgtggacaa ggtcctgatc accgccggaa
     3781 gggtcgaggt ggagttcggc ggcggccggg tcctggagcc gccgacggtg atcgtcgggg
     3841 gaagggccac cccgccggcg ggggaggatc tggaaggcct catcgcgagg gtcgcctccg
     3901 accacctaaa gcggtccctc gcgaacctgc tcgagcaccg gatcgacccc agggtgaggg
     3961 gagggtcgcc ggagctcgta agcctgatga gacggggggc gaacgacacc tcgatcgggg
     4021 tgggttttgc tcccctcaac cccaccgagg agctcgtcct ggagctggag gagagggtcc
     4081 ggatggtcag gggggtcggc gaggacacca aggttatggc ggtgaggatc cgcgatcgtc
     4141 tgaacctggt ggtggcggcg gcgatcgtat cgaagttcgt ctcctcgatg gaggagtacc
     4201 gggaggtgaa ggagagggtt cgggagacga tcgccacggc ggcagacgcg gaggtcgccg
     4261 tcaacgccgc cgacggagga aacaacatct tcctcaccct gacgggctcc tcgatagaga
     4321 tgggggacga cggcgccacc ggccgcggaa accgggggag cggcctcatc acccccatgc
     4381 ggccgatgtc catcgaggcg atcgcaggaa agaatcccgt aaaccacgtg gggaagatct
     4441 acaacgtcgt ggcccaaagg gcggcagagg agatcgccga gctcgagggg gtagaagagg
     4501 cttacgtcac cctggtgagc aggatcggcg ctcccctcac cgaacccctc ctcagggctg
     4561 tggagataag aggagacgag cggccgggcg tggtcgcgga gaagaggatc gaggcgatag
     4621 tcgaccggca cctccagggg atcggagacc tggtcgagga tttcgtgggg ggaaggctgc
     4681 gggtctgcta gatataggtc tgtaaaaggc ggaagaccga ctgcttcgag taaaagccgg
     4741 gcggctgagg ctgtaaagat gagcggactc gggagagggg ccctagccct tgctcacttc
     4801 tttcttcgcc tgctggagcc gctgctttgc ggtgtagccg gtggcccgct ccaggaagtc
     4861 tcgaaatctc ttgttggtgt cgaggatgac ctcgtcggtg acctcgtcgg tgaccccgtc
     4921 ggtggactcg gtggtgacga ggagcttctt ccccacgatc gagacctcga tggacttgag
     4981 ggcgccgtag ctgagaagaa gcctcccgcc ctcctttcgg atctcggtgg gaaactcctc
     5041 ctccagggcc gacctgatcc tctcaagctc ggggctgaag cccctcttga acgagtattc
     5101 catggaccgg cccttcgttt cattcgatta tagacctttc gagccgcacc ttcgagccgc
     5161 cgcgctgccg tctcagatcc gggcgtagct cgcggcggag ccgagcgcct cctcgatccg
     5221 gaggagctgg ttgtacttgg cgttcctctc ggaccgggcg ggggcgccgg tcttgagcat
     5281 ctcggctccc agggccacgg tcacgtcggc gagggcggag tcctccgtct cggcggacct
     5341 gtggcttgcc ataaccttaa agccgtttct gaaggccatc cgggcggcgt cgaaggcctc
     5401 ggagacggtc ccgatctggt tgagcttgag caaaagcgcg ttggtcgcct tcatctcgat
     5461 ccccctggcc agcctctgga cgttggtgac gtagatgtcg tcgccgacga tgatcgtccc
     5521 cgggaggcga gcggtcaggg cggcgaagtc cccgaaggcc tcctcctcga aggggtcctc
     5581 gatgaggatt atgggataag tcgaaaccag gtcggcgtag aagtcgacca tctcgccgcc
     5641 ggagagcttc ttgccgtcga tggcgtaggt cccgttttca aagaagctgg aggcggcggc
     5701 gtccatcccg atcccgatct ctttatcgta gccggtgacg tcgagggcgg acatcaggga
     5761 gtcgagggcg tcgactgttt tgttcagggg cggggcgtag cctccctcgt agccgacgtt
     5821 ggtggccccg acgccgtacc tcccccggag gatcttgccc agggcgtggt aggtctcggc
     5881 cgccatccgg aggccctcgg agaagcagct tgcgcccttg gggaggatca tgaactcctg
     5941 gatggcgagg tcgttaccgg catgctggcc gccgttgagg acgttcagag acggcacggg
     6001 gagcgtagtc gtgttgaccc cgccgaggta ctggtacagc gggatggcca gctcgtcggc
     6061 ggcggccttg gccacgccca aagagacccc cagaatcgcg ttcgcgccga ggttcgactt
     6121 gaggggggtg ccgtctaggg ccagcatgaa gttgtcgacg gcccgctgct cgagagcggt
     6181 gatcccggac tcgatcagct tcggcccgat gatctcgttg acgttcttga ccgcctggag
     6241 gacgcccttg ccgccgtaac ggtcgtcgcc gtcccgcagc tccagggcct cgtgggttcc
     6301 cgtcgaggcg cccgagggga cggcggcccg gccgaagccg cagcaggtcc agacgtccac
     6361 ctctaccgtg gggtttcctc gcgagtcgag gatctcgcga gcgtgaatgt tctcgatctc
     6421 aaatgacatc ttatatcgcc attcgagcct tcgtggccga tttatttaac ctctttggga
     6481 ggtcggaaaa gatatgtccg ccagctgtgc tgctggccgc cggccagctt tgagactttt
     6541 cagccgttct ccagccggag gaagtgcacc ctacccgaag attcgccagc gacgatggtc
     6601 ttcccatagg gagctatggc gcagcaaagg atcgaaccgt cggcggtgaa ggtggcgact
     6661 attttcccgg tttccatgtc ccacaccttg agtgtctgat catcggaagc agataccgct
     6721 tttcgaccgt cgggggtcac cgccaccgcg tagaccgaac ctgaatgacc tttcagggtc
     6781 cgcacctctt cgccccgctc caggtcccag accttgaggg tctgatcatt ggaagctgat
     6841 accgctttcc gcccgtcggg ggtcaccgcc accgcgtaga cccaacctga atgacctttc
     6901 agggtccgca cctcttcgcc ccgctccagg tcccagacct tgagtgtccg atcaccggaa
     6961 gcagataccg cttttcgacc gtcgggggtc accgccaccg ccctgaccga acctgaatga
     7021 cctttcaggg tccgcacctc ttcgccccgc tccaggtccc agaccttgag tgtctgatca
     7081 tcggaagccg ataccgcttt tcgaccgtcg ggggtcaccg ccaccgcgta gaccagacct
     7141 gaatgacctt tcagggtccg cacctcttcg ccccgctcca ggtcccagac cttgagtgtc
     7201 tgatcaccgg aagacgatac cgcttttcgc ccgtcggggg tcaccgccac cgcgtagacc
     7261 caacctgaat gacctttcag ggtccgcacc tcttctcccc tctccaggtc ccagaccttg
     7321 agtgtccgat caccggaagc cgataccgct tttcgaccgt cgggg
//
LOCUS       DASM01000092            2719 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_136700, whole genome shotgun sequence.
ACCESSION   DASM01000092 DASM01000000
VERSION     DASM01000092.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_136700"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          521 a          833 c          814 g          551 t
ORIGIN      
        1 tcggggtcct cggtgagggc gatgtggctc tcgggccgcg aggaggacat cttgccgccg
       61 gtgaggccgg tcatgaaccg atggtataga gacgctggag ggacgaagcc gtagccgccc
      121 gcctccacct ccaggctcct catcgcctcc tcgaccctcc ccaagtcctt cgccaaggcg
      181 tcgaagacgt cgatgtgctc ctcgtaccgc ttgaccttcc tgtagcccat ctccttcagc
      241 cgggcggcgg cggcatcgat cagctcggtc cccgccgcct tcccccggag gctgatgcgc
      301 ccctccctct cctctaccag gaactttctc atcctcccgg ccaggtcccg ggtgagccgg
      361 atgtgggcgt cctgatccga gccgaccgga atcaccacgg gcttcggccc cccgttctcg
      421 gggagctggg ggtggaggat gtcggcactc tggaccatga ccgagaccat gtgggcgacg
      481 ttcgtctccc cggagaagcc gtatatcgcc gccagctccg agaaccgagc ggcggccgcg
      541 agctcgaagg cgaggtcccg gacgtccccg cattccgact ggaagtatat ggtcgccgac
      601 ggctcgagcc ccagggcgat gatgctgagg atgtactcct cgattcccat ctcccgacac
      661 cgctgccagc tgatcccccg gaccgagtgg gcctccatgt cggcgatggc cacgaaggcg
      721 tcccccccgt gcttctggtg ccagatgatc tcctccatca ccatcttgcc gccgagatgg
      781 accctcccgc tgggcatgaa cccgctcatc acggcgaagg ggctcttctt cttcatcgcc
      841 tcgacgaccc tatgataatc gcggtgacca aatattatat gcctcttcat atatggatgg
      901 gggtcatgga tctggggcag gagatcctcg aagctcgata tcccgaactc ctcgaatagc
      961 ttcgagtagt cgtctatcga gaccgcgccc caggggtcca gcttcgttgc catacgtcca
     1021 ggcgatgcag aatatggtat atttaccttc tcgtcgtcct cagaaggccg tcggtcggga
     1081 ggagggtccc agctaaaatt atttaaataa ggaagtcgat gatactttcg atggacctag
     1141 actccttccc ctgcgatctc gtgacctgga atgagttcta cgaccttgcc cggactttat
     1201 ccaggatcgt caaggcttca gggtggcgcc ccgacctggt ggtggcgata ggccggggag
     1261 gctacgtccc ggcccgggtc gtctgcgact ttctggtcca cgacctcctc accagcatca
     1321 aggtcgagca ctgggggatt gcggcccaga ggagggaaag ggcatcggtc cgtttccccc
     1381 tggcgaccgc agcctccggc cagagggtcc tcgtcgtcga cgacgtcacc gacaccgggg
     1441 agaccctggc ggcggcgacg gggtacttga agagcctggg ggctggggag gtgaggaccg
     1501 cggtcctcca gcacaaggcc agctcaacga tcgagccgga ctactacgcc gagaggatcg
     1561 atctatggag gtggatcata tacccctggg cggcccacga ggacctggtg ggtttcaccg
     1621 gaagggtcct ctctggggca ggtcgccccc tctcgaaggg ggagatcatc gcagaattga
     1681 agaggaggtt tgagatcgat ctggatcagg aggagatggc cgaggtccta gaggacctcg
     1741 tctccctggg gatggccatc aggtctggat cggtttatac cgccacaaga tgctgattag
     1801 attggggatt tggataaaca aaaataacct atttatatga gaatgatttt gtaaccagaa
     1861 aaatagctgt ggtatttatg agacaatttt cggtcaaagt cttggacgac gacgaccggg
     1921 agttcgtcga ggtcctccgg gacctgggca tcccccggaa cgtcgccagc atgatcacct
     1981 acctcgccaa cgtccaggag gctacctcca gggagatcga gatcggatcc aacctccgcc
     2041 agcccgaggt aagcatcgcc atgagggccc ttcggaacaa cggctgggtc gaggagcggg
     2101 aggtcaagaa ggacgggaag gggaggccga tgaaggtcta ccggctgata aaatctcttg
     2161 aggagatcat aacccacttc gaagaggaga agaggctgga gtctacgcgg gttatggaag
     2221 acatcggccg gctgaaggat ctgagcggaa ggctcagcag catctgaaaa tctcctcatc
     2281 cagaggagac gacccggacg atctccaggt ctttggcctc caccagatcg tcggcgggaa
     2341 cggggtggcc ctccaacagc gccaccaccg tctcgggttt tatattcaga agcatgaaga
     2401 gctggtcgta ggtcaggtcc ctatcgagat cgacggtcct ctcggtgacg aggccgtcgg
     2461 cgcggatccg gatcttgagg gctctcactc ctccttctca ccgcctgggc ccacgagatc
     2521 gtcgaggttc tcctctatca gcttcttctc caggttctcc gtcttcttgt cgcccttcct
     2581 ctctacgtcc cttttcttga acttgggcac cagcatcacc cctccgtcct ttcgatctcg
     2641 ggcggtatta acctttcgtc accctagcct ccgccctagg agagcccact ggtcgtagat
     2701 ccccaggtcg tcggggttc
//
LOCUS       DASM01000093            7630 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_43672, whole genome shotgun sequence.
ACCESSION   DASM01000093 DASM01000000
VERSION     DASM01000093.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_43672"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1562 a         2180 c         2601 g         1287 t
ORIGIN      
        1 tggggaacga gaaccgcaga aggatcctcc agctcctctc ctgccgcccc ttctacttca
       61 acgagatggc caagcggctc gacctcgggc cgaaggcggt gatagaccac ctggagatgc
      121 tggagcgggc ggggctcatc gagtgctacc gggacgaccg gcgccggaag tacttcagga
      181 tcgccgagaa cgtcgtcctg gaggtggcct tctcccccca ctcctacggg atcaggagct
      241 accgccccca gccgcccgga aggccgctga ggagggaggc ctggggcgat aggataccgg
      301 accggacgcc ttcggccccc gaggatccgg ccggaccggg cccgggcagt ctccggccta
      361 atgagctgga ggggatctgc tcgcgcctcc gggagctgga ggaggagcgg ctccgattgc
      421 gggggctttt ggaggagata gcctccgagg agatggcggc gaggcggctt gctacggggc
      481 tcatcgtcga gtccgcctcc gaccagatgg aggcggagat cctcaccgcc ctcctctccg
      541 gcgactccag ctcggaggcg ctggccgcaa ggctggggat ccccgctgcc gtagtcgagg
      601 agaggctgag ggggcttgcg gcccagggga aggttcaaaa gcggggcgag cggtggacga
      661 tatgaccgat aacggatctc gatcagcatg accatgacca caactctcag ggtggcggag
      721 gcctatcaca gggatgtcgg ccgggggatc gccaggctcg cccaggatct gatcagcgat
      781 attggcgtcg aagggggcgc ggtggtggag atccagggga ggaggaccgc ctacgccgtc
      841 gcctggcagg cctcgccgag ggaggcgaag gacgtcatca ggatagacgg gaacctccgg
      901 agcaacctcg gggtaggtat agacgaccgg gtgaccgtcc ggaagagcga ggcgaggccg
      961 gcgaagagga tcgtcctcgc ccccaccagc aagacgagga tcgtcggagg cccccagtac
     1021 ctcctccgga tcctcctggg caggccgatc gtgaaggggg agcagctgaa gatcgagatg
     1081 atctcgtcag ccctcggctt cgtcgtggtg agcaccgccc cgaagggtcc ggtggtggtg
     1141 gctgccgaga cggagatcaa gatcctgaag gataccctgg aggagatggc ggtgagggac
     1201 gtctcctacg aggacatcgg aggcctcgga aaggagatca ggatggtccg ggagatgatc
     1261 gagctccccc tccgccaccc cgagatcttc gagcggctgg ggatcaggcc tccccagggc
     1321 cttctcctct tcggcccgcc ggggacgggg aagaccctca tcgcccgggc ggtagcgagc
     1381 gagacggagg cgaacttcat cagcatcagc ggccctgaga tcacctccaa gttctacggc
     1441 gagtcggaga agaggctccg ggagatcttc gaggaggcgg agaggagcgc tccctccatc
     1501 gtcttcatcg acgagatcga ctcgatagcc ccgaagcggg aggaggtggc gggggacctg
     1561 gagcggaggg tcgtcgccca gctcctctcc ctcatggacg gcctcgcctc ccggggcgag
     1621 gtgatcgtca tcgccgccac caaccgcccc aactccctcg acccggcgat ccggcggggg
     1681 gggaggttcg atcgggagat ggagatcggg atccccgaca agaacgggag gctcgagatc
     1741 ctctacgtcc acacccgggg gatgcccctc tcggaggacg tggacctgga ggggatcgcc
     1801 gagaggaccc acggcttcgt cggagccgac ctcgcctccc tctgcaagga ggcggcgatg
     1861 cacaccttga aggggctcat ccccgacctc gacgcggagg aggcgatacc cctccgggta
     1921 ctggagggtc tggtggtggc ggaggaggac ttcaggttcg ccctaaagat gatcgagccc
     1981 tctgccatga gggaggtctt tgtggaggtg gcggaggtcc actgggacga ggtcggcggc
     2041 ctggaccggg cgaaggagga tctggtggag gcggtggagt ggcccctcaa gtaccccgag
     2101 gccttcgact cggtcgggat caggccgccc cggggcgtcc tcctcttcgg cccgccgggg
     2161 acggggaaga ccctcctcgc caaggccgtg gccaccgaga gcggggtgaa cttcatctcg
     2221 gtgaagggcc ccgagctcct ctccaagtgg gtcggggagt cggagcgggc tgttagggag
     2281 gtcttccgga aggcgaagca agcggctccc tccctcatat tcttcgacga ggtcgatgcc
     2341 gtcgtccccg ccaggggctc ggggctcgac tcccacgcca ccgagagggt ggtgagccag
     2401 tttctgacgg agctggacgg cgtcgtcgag ctgaaggacg tcgtcgtcct cgccgccacc
     2461 aaccggccgg acctcttgga cccctccctc ctccgcccgg gaaggttcga ccgattgatc
     2521 aggatcccga tccccgatca ggaggcccgg gagcggatat tcgagataca cctctcaggg
     2581 atgcccctgg ccggggacgt ctccgcctcc cacctcgcgg aggtgacgga ggggtggacg
     2641 ggggcggaca tcgagaccct ctgccgggag gcggggatga ccgccctgag ggagaagatc
     2701 ctccccggga tgaggcgaga ggagctgatc ctcgaggggg tccaggtgga agggcggcac
     2761 ttcgggcagg ccttcgagag ggcgaagccc cacctgaccc cggagatggc ggcggattac
     2821 cagaggctga tgaaggattt cgaggtgtag aagcgaattg acgtctcagc atcagttatt
     2881 taggctagaa ggtttaccta tggtcgagag ggccgatatt ttgttggacg atgagaagaa
     2941 ggatctgttg ggcagcaccg agttcgagga caccagcacc gtcaaggtcc ccgagagcct
     3001 gatcgaccag gtgatcggcc aggacgaggc ggtggaggtg atcaggaagg cggctcacca
     3061 gcgccgccat gtgatgctga tcggctcccc gggcacgggc aaatcgatgc tcggaagggc
     3121 gatgagcgag cttctccccg tagaagacct ccaggacgtt ctggtctaca acaaccccga
     3181 ggagaacaac aaccccaaga tccgggtggt gccggcgggg agggggagac agatagtcga
     3241 cgcccacaag atggaagcgc ggaagaagac ccagaccagg aacatgttct tcatgctgat
     3301 catgatgggc ctcctcgttt acgcctacta catgggccag ctcctcttcg ggatcatcgc
     3361 cgccgccctg atattcctca ccatgaggta cctcatcccc aaggacgact cgatggtgcc
     3421 gaagcttctg gtggacaaca gcaaacagaa gaaggcacct tacgtcgacg ccacgggagc
     3481 ccacgccgga tccctcctcg gcgacgtccg gcacgacccc ttccagtccg ggggcctcga
     3541 gaccccgagc cacgagaggg tcgagtctgg cgccatccac cgggcccaca agggggtcct
     3601 cttcatcgac gaggtgaaca ccctccggct tgagtcccag cagagcctcc tcacctctct
     3661 ccaggagggg gagtacccca tcaccggtca gagcgagcgt tcttccgggg ccctcgtcag
     3721 gaccgatccc gtcccttgtg atttcgtcat gatcgctgca gggaacctcg acgccatggc
     3781 ggggatgcac cctgccttga ggtcccggat caagggttac ggctacgagg tctacatgag
     3841 ggataccatg gacgacaccc tcgagaaccg ggagaagctc gtccggttcg tcgcccagga
     3901 ggtgaacagg gacgggaaga tcccccactt caccagggac gccgtcgccg agatcgtccg
     3961 ggaggcgaag aggagggccg ggaggaaggg ccacctcacc ctcctcctcc gggacctcgg
     4021 cggcctcatc cgggtcgccg gggacgtcgc ccgttccgag ggggcggccc tcactgagat
     4081 gaggcacgtc ctggaggcga agaagatggc ccggtccgtc gagcagcagc tcgccgacat
     4141 gtacctggag aggaggaagg actactcgat gttcaagaac tccggagccg agatcgggag
     4201 ggtgaacggc ctcgccgtcg tcggcgactc cgggatcgtg ctcccaatca tggcggaggt
     4261 gaccccggcc cagtcgaagg aggaggggaa ggtgatcgcc acggggaggc tccaggagat
     4321 cgcaaaagag gcggtccaga acgtctcggc cctgataaag aagttcctcg gggaggacat
     4381 caccaacaaa gacgtccaca tccagttcat agggacctac gagggggtcg agggggacag
     4441 cgcctctata tcgatagcga ccgcggtgat atcggctctt gagagcgtcc ccatcaagca
     4501 gaacatagcg atgacggggt ctttgtcggt gaggggagac gttttacccg tcggcggcgt
     4561 cacccagaag atcgaggccg ccgcccaggc ggggatcacc accgtcctca tccccaagtc
     4621 caacgagggg gacgttctca tcgacgagac cttcagggac aggatcaaga tcatccccgt
     4681 cacctccatc agtgaggtct tcgagtacag cttctcgggc caaaagccga agcttttgga
     4741 gaagctgaag aagttcgccg cggagaagaa gctggggatc gtcctccccg agacgatccc
     4801 gaccccgagc aaaggcctct gatcatcggc ggcgaagagt tctccggggc tggatggaga
     4861 tgctcccttc ggcatccctc ggagcctcct ctttttattg gtcagatccc gcaaggcagt
     4921 ttcttccccg gagcatagac agggttaagt cgtaccaacg ccctcctgcc gtcgagaagg
     4981 gctggagatg gagatcagat gggcgagttt cacgatcagc gggtgataag gggcaaagcc
     5041 accaggatag tcctcgacaa cggggagctg gacgagatct cggagagctt ctttgagggg
     5101 gcggccatca gggccctcgc gggcgggtcc tggggattcg tccagaccga gtccctcgac
     5161 gaccttttgg gggatctgga gagggcgagg cgagcggccc ggaggatcgc aggagacgag
     5221 aggctggacc tcgtgccctc catccccgga aagagcggca aggttccagt aaagaaggat
     5281 ccagcgagcc tatctcttga ggagaaggtc gatctcctcc gggagatcga ggcggcagcg
     5341 aaggtctcgg gggtatctag cacccaggcg gtctacaacg aggtgctggt gaagacgaat
     5401 tacgccagct ccgatggccg ggacctggag gcggaggcga cgagggtcgg cttctacgtc
     5461 accgccgtgg cgagccgcgc ggggctgtac caggcgggga tggagagcag ggccggatct
     5521 tgcggcctgg agctcttcga ggatgtcgat gcggtgggcc tcgcccggcg ggcgggggag
     5581 acggctgttg ccctcctcga cgcgaaggcc ccccggggcg gggtctatcc cgtcgtcgtc
     5641 gaccaggagc tcggcggcgt cttcgtccac gaggcggtgg gccacgccgc cgaggcggac
     5701 atagtcctcg agggcggatc gatcctggag gggaagatcg gcgagcggat cggctcggaa
     5761 ctggtgaccg tcaaggacga ccctagcctt cccctctacg ggttctaccc cttcgatgac
     5821 gaggggagcg gatccggcga gacggttctg gtggaagacg gcgtcctcac ctcctacctc
     5881 cacacccggg agacggcagc ccgcctgggg ggggtccctc ggaacgccag agctcaagga
     5941 aactcgaggc ccgtggtgag gatgtccaac acctacatcc caccggggag ggacggctgg
     6001 agccttaagg agatcctgga ggagatgaag gatggggtct acctcttggg gagccggggc
     6061 ggccaggttt ccaccgccga ggggatcttc cagttcaacg ccaagcgggg ctacctcgtc
     6121 tccggaggcg aggtgacgac cctccttcgg gacgtatccc tttcaggcca catcctcgag
     6181 atccttgaga gggtgaaggc ggtcggggac gacctctcct tcaacagcgg aaggtgcggc
     6241 aaatcgggtc agctcgtccc cgtcagcgac ggctcgcccc acctcctcat cgaggaggcg
     6301 gcggtggggg gttcgggata ggtcaaagtc gtgtggcagc gatcaattcc agcgatcatg
     6361 atatatgtcg ggagttttat caggcaatgg tggacttatg ggggcgggcc gaacgaagtg
     6421 cgatcataag atctgcgggg acaaggagaa agtatggcta cctcactcat ttagggggcg
     6481 agggcgtgga cttcagcctc accgctactg cgtcaattgc ggccttgtgg aggagcattc
     6541 tgacgaaaga cctctctcca tcggccatta tatgaacgcc ctcgccagga ttgggatgga
     6601 gttcaaggtg gcgaaggtcc agacgaggat gatatcgatg gagatggaga ggtgcagcct
     6661 cgacgacccc tatggcatgg acagacgaca gcaagaagat ctcttcgtcg gcattgtagg
     6721 aacatatttg aatttatctg aagaacggat tcgcaagttt ctttgatcca gatcctcgcc
     6781 tgggctgaat tgatgccatg ataagaggcc aggcgatcat ggggcagtca agccgcataa
     6841 aaaacaaaaa aaatatagat tatatgtttt taaatagtcg aggagaggat cggatatctg
     6901 acggagttgg tcgatgtgag gatttcggta ataggtggtg gatatgtggg cctggtatcg
     6961 tcggcatctt ttgctgagct aggccataat gtaagtatta tagaaataga caagtataaa
     7021 attgaaacga taaactcagg aagggcgccg atatacgaga aaggtcttga tgatctcctt
     7081 tccaaacatg cgggcaggaa cctgaaagca actgatagct acgatatggt cgccgattct
     7141 gatctctcct tcatatgcgt gggaactcca cccgggcccg atgggggcgc cgacctctcc
     7201 atggtcgcct ctgccagccg atccataggt agaaatctga gggataaaga aggatatcat
     7261 gttgtagtcg taaagagcac cgttcctccc tgtacaacgg agaagctggt ggtgccgacg
     7321 gttctggagg aggtcggctg cagatccgat gatatcggct tcgccatgaa tcccgagttc
     7381 ctgagggaag gcatggcgtt ctacgatttt atgcatcccg acaggatcgt gatcggaagc
     7441 tcggaggaag aggcgggaga tctcgtagaa tcagcgtact ccgggataga ttcgcccgtc
     7501 caccgggtgg ggatcaaagc tgcagagatg ataaaatacg cttccaacgc gatgctcgca
     7561 acgaagatct ctttctccaa cgagatcggg aatatatgta aagatctcgg catagacgtc
     7621 tacgaggtga
//
LOCUS       DASM01000094            7271 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_74590, whole genome shotgun sequence.
ACCESSION   DASM01000094 DASM01000000
VERSION     DASM01000094.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7271)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7271)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7271
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_74590"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1224 a         2508 c         2240 g         1295 t
ORIGIN      
        1 cggcgagggt cttgccgcag gccggtgccc tctcctccac cttgacggtg acgacctcga
       61 cgttggggag gtaccgcctc aggtccgaga tcttcgacgg ctcgatctcg gcgttgagga
      121 acatttcgta gccgagggac cggacctcca cgatcatctt ctcgatctcg gtcctgggga
      181 cgaggtactt ggcgaggacc cgggagaaga tccccaccga ggtctcgaac tcctcgggga
      241 cgacctcgtc cgcccccagg tcgtagaggg gnntcctcct cgccagctcg acgatcctcc
      301 gggtcgccat ctcgtcggag acggcgacga cgacgaccct cgccgtatcg acgtcggcgt
      361 gccgcaggac gatctcctgg gtggcatcgc cgtagtggat cggcacccct ttcgccctct
      421 ctcccctcac cttctccggg ttcatctcca ggaccatgta ggtgagctct cctaccctcg
      481 cggctttggc cagggtcctt ccgacgaagc cgtagccgac gatcaccagg tggttctccc
      541 tcttctcagg gatcgcggcg aaggggaggc ccgacctcag cttctccggc atggggaggc
      601 gataaatgat ctccgagacc ttcggcgagg cggcgatgac gaagggggtt gccgccatcg
      661 taagaatcga gaccgccagg aagagctggt actcgccccg gcccatgatc ccggcgtcga
      721 ggccggtctt agataggatg aaggagaact ccccgatctg ggcgagggcg agccccgcca
      781 ggacccccgt cctgatgggg tagccgagga ggacggatac cccgccggcg acggcggcct
      841 tcaacagaac gacccccagc gccgccgcca ccaccagctc aaagctctcg aagaagaagc
      901 cgacgtccag gagcatcccg atggagacga agaagaagct ggtgaagacg tccttgaagg
      961 gtattacgtt ccccagggca tgatagctgt aatccgactc ggagatgatc agccccgcga
     1021 ggaacgctcc cagggcgagg gagaggccgg cggagtagga gacccaggcg acggcgaggc
     1081 agatgaggat caccgtcagg aggaagagct cgctcgaccg gaggcggaca acctggtaga
     1141 ggagggcggg gacgatcagc ttcgcgctga ctatcaccag gacgactatt ccggcgccct
     1201 tcgccagcat catcaggagg gccgccgata ggcccgtcga ctcctccgcc gccaggaggg
     1261 ggacgaagag catcatgggt acgacggcga tgtcctggaa gatcagtatc cccagggtgt
     1321 ttccgccgtg gggggtgtcc acctcgtccc gatcctggag gatcttgagg accaccgcag
     1381 tgctgctgag ggcgatgaga aatccggcga agatcgattc gttgacgggg agccccagtt
     1441 cgatgccgat gagggtggtc gcaagaaggg ttatggccac ctggaggggc ccccccacca
     1501 gggccgccct tctgatccgc aggagcctct cgaaggagaa ttcgatcccg atggcgaaga
     1561 ggaggaggac gacccccacc tcggagaaga actctatctg ctccacctcg tggacgagcc
     1621 ggaggccgtg ggggccggcg acgatccccg tcaccagaaa ccccaccacc accggaaccc
     1681 ggagccttgc ccctacgaag atggcgatga tcgaaatgag gaagatgatt atgatgtcgt
     1741 tgaggatggg tatgggcatc cttcagccca tcatcatcat ccccgttaaa gcttagggtc
     1801 ggaggatgga tggggacgca tataagcaga agagagaaga agtcaggacc gaggacgccc
     1861 ccctcccggg agggcgccac cggggcgggg gacggcgccg ccgataagat cgatggtcag
     1921 cggccgccgt aacccgatgt cgtcatcgag attatacgca gccgtcgagg agcctctttc
     1981 ttccaccgcg cttctcactc ggctttatcg ggtccacctt tgctacctct tcctctttct
     2041 tctccttctt cttctcttcc gccatggaaa tcacctctgc cattaattcg gctgtactcg
     2101 tttattaaac tttccgatac cgtggccctt ccttctcgat ctggtggcct aaaccttcca
     2161 agctccatct caaaggtcga ttctgggggt taaatcacgc ctctctcctg gcgattggga
     2221 gacgatgtcg gatacgtcct cgagaggaca gaaaaaaatt ggcttatgta tctgggatga
     2281 tccaggagga gctcacttcc ccaaaaggtc cttcaccacg caggtcgggt cctccccgca
     2341 aacgtctccg cagtagtacc gagccttctg ccggcagccg ccgcagacgt ccaggtactc
     2401 gcagtcgccg caggccccgg tgagcttaga ccttatatcc ctcagggcca ggagctccgg
     2461 agagggatcg tccatccaga tctcccggaa gctcctctcc cggacgttcc cgacgaccac
     2521 ctcgggcatg aagtggcagg ggaggacgtc cccccgatgg tttatgttgg cgaccttcac
     2581 cccggcggtg cagcccccgg cgttcttgag gagctggcgg acccgctccc gcctctccgg
     2641 ctcgtccttc agcatctcca ggaggtaagc cccgtccatg ggggagtcgg tggagaggat
     2701 ctcgatctcc cggtccttca gctccaccgc cgcctccgcc aggaacctga tgacgctccg
     2761 cctctcctca ggggagacgt cccggtcgga gatctccgcc cccctcccca cggggacgag
     2821 gtggtagagg cagaacctcg gaacctccag ctccagggcg aggtcgagga gggcggggac
     2881 ctcgtgccag ttgtccttgg tgagggtgac cctcaggccg gtcttgaggc cggcttcccg
     2941 ggcgtttgcg agcccctcga tcgccctatc gtaggcgccc ttcaggcccc ggaatctgtc
     3001 gtgggcctcg ggcctgcagg agtcgaggga gaccccgacg tacctcacct ccgcctctct
     3061 gaggaggcgt gccacctccg gggtgatgag ggtcccgttc gtcgagatga ccgtccgcag
     3121 gccgacctct ttcgcatgaa atgcgtaggc gtagaagttt ctgctcagga ggggctcccc
     3181 tccggtgagg atcagcatcg ggatcttgag ggcggcgagc tcgtcgatga gccttatacc
     3241 ctcttcgagc ttcagctcct ccgccgcggc ctccgtagcg tcgatgtagc agtggtcaca
     3301 attgaggttg cagtggcggg tgatgttcca catcaccacc ggcttcttct ctcccgagaa
     3361 cctgatcatc tcgtcgggga cgggcccttc cgccccttct gctctcctga tggagtccca
     3421 gaccgtcgcc tggtccgcca gggccttgga gaaaataatc atcctctcct cctcagctct
     3481 tccagcatgg caccgaaggt gtacctatcc ggcatcacgt ccaccctcac cccgagttcc
     3541 tccagaacgg ccctggtggg ggggccgatc gccaccacct tcgcctcgga gagcctctcc
     3601 cggaggcggt cctcgccgcc gagctccttc gacctcgcca ggaggtttct ggcggtggag
     3661 ccgctggtga aggcgaagac gtccggggtc ccccggagga tcagccagtc gagcctggag
     3721 tccccggagg gggcgatgtc gtagaggtgg acctcgtcca cctccacccc cgcctcttcg
     3781 aggatctcca ccagctcggg gcttcccttg gcgctccgga ggaggaggac cttcttgaac
     3841 cggggcgcga gagcgagggc gagcccccgg gagctgaagc tctcggggac gatggacgcc
     3901 gagagacccc gttccgccag ggcctcctcc gtccggggtc cgatggtggc gaggaccgac
     3961 ctcctcagaa gggacgcgag cccatcgtcc cggagggcga tctccgcccc ggtggcgctg
     4021 gtgaagacga cgcattcggc cgcctcgatc ctctcgggga ggtccttcgg cagcggaagc
     4081 tccaagaagg ctatggaggg ggcgaggatc ggcctgaagc cgagggatat ggcgatcctc
     4141 tcggactcct ccccccggtt gtccggcctc aggatggcga tcgtcggctg cctcagcctc
     4201 tctgataggg agaccacctc ccccaccacc aggatcgccg gggcctggac gtcctcctcc
     4261 ttcgccctct cgactacgtc tccaagggtc ccgacgacga ccctctcctc ggagctccac
     4321 cccctctcga tgagggcggc cggggtcttt gggccgcggc caccccggag gagggcggag
     4381 acgttctctg cgagccttga tacccccatc aggacgacga tggtcccgcc gacccgggcc
     4441 agggcctccc agtccagctc ctcctccttc cccggctcct cgtggccggt gacgacggtg
     4501 aaggccgtcg ctacgccccg atgggtgacg gggatccccg cccctgccgg gacggatatc
     4561 gccgaggtga cccccgggac gacctcgaag ggcaccctcg ctgcggcgag ggcgagcccc
     4621 tcctcccctc ccctcccgaa gacgaaggga tcgcccccct tcagtcttac gacgatctta
     4681 ccttccctcc ccttctccac caggagggcg ttgatctcct cctgagagag cttgtggcgg
     4741 ccggggagct ttccgacgtc gatcatctcc gccccctccc cggcccatcc gaggatctct
     4801 gaggatagga gccggtcgaa caagatcaca tcggcctctt ctaggagccg cctcgccttc
     4861 agcgtcaaaa gctccgggtc cccggggcct gcgccgacga gccatacctt cccgcattca
     4921 gatcgatcca acttccctca ccgcctcttc tgccagatcc cgtccgccca tcgccaccag
     4981 ccttctagag agctccctcg ccccttccag gtggtcggcg gcggagatcc gtccctccgc
     5041 cctcaccgac ctcgccccat ccagggacaa gacctccgcc aggaccgaga tctcgtcccc
     5101 ggagagccgg gcgaaggcgg ccatggggac gacgcaggag ccgccgatct cttccaggat
     5161 cacccgctcg cactccgcct ccctcctcgt cgggaggtgg tcgatcccac cgacgagctc
     5221 ctccgccctc ccttccgtcg ccaccacggc gatcgccccc tggtttgcgg aaggtacgaa
     5281 cctctcgggg tccaggacgg agtatcgagg acggtagccc atcctctcga tcccagccag
     5341 ggcgaggacg atcgcccgat acctcccctc ctccagcttc cggagcctcg tctccaggtt
     5401 tccccggagg gactcgatga ccaggtcggg cctcgcccgc cgcagctgcg ccgccctcct
     5461 catgctgcag gtcccgacga ccgccccctc ctccagcccg tcgatggaga cgccgtcgtc
     5521 gctcaccagg acgtccaggg gcgagtcccg ggggaggacg gctccgatga cgagcccctc
     5581 gggcctcttg gaggggaggt ccttcatgct gtggacggcg acgtcgatct cccccgccag
     5641 catcaggtcg tcgatctcct tgacgaagag ccccctcccc gagacccggt ggagggggag
     5701 gtctaggacg tggtctccct tcgtcttgac gaccttcatc tccgcctcga cccccctcgc
     5761 ctccaggagg gagatggcgg tcctcgtctg ccgcagagcg agagggcttc cgcggcttcc
     5821 tacgatcagc ctatcaggtt tgacttgtaa gcctccagag tctcgtttat gacgtcgtcg
     5881 gtgtgggcgg cggataggaa gttcgtctca tactggctcg gcggcagaaa taccccggcc
     5941 ttcagcatcc tccggaagaa ggcgaggtat cccgccttgt cgcacctcag ggcctcagcg
     6001 tagttctttg gagcggcgcc gaagaagatc ttgaagatgg aggccgtccc caccaccgag
     6061 tagctgaggc cgaggtcctc gacgatctgg gcgagggcct tccggagatc ctcccccacg
     6121 gagtccagcc tctccagggt cctctccccc tccaggacgt cgagggtcgc catcccggcg
     6181 gcgagagaga cggggctgcc gttgaaggtc cccgcctggt agatcccgcc gctgggggcg
     6241 accatctcca tcagctcacg cctcccgccc accaccccga tggggaagcc tccgccgatg
     6301 atcttcccca gggtggtcat gtccgggacg acgccgtacc gggcctgggc gcctccgagg
     6361 ctgagccgga aaccggttat cacctcgtcg aagatgagaa ggacgccgtg ctcctctgtg
     6421 agccttctcg ccccctccag gtaccccggc tcggggagga cggggccgat gtttcccagg
     6481 accggctcca tgatcaccgc cgccacctcg ccttcgaagc ccgtcagggc cgcctccatc
     6541 gccccaaggt cgttgtaggg gacctggatg gtgttcttgg ccgtatcctt cggcaccccc
     6601 agggagtcgg ggacccccag ggtcgtcgcc ccggaccccg ccttcaccag gacggcgtcg
     6661 tgggcgccgt ggaacccccc ctccaccttg acgatccgat ctttgccggt gtagccccgg
     6721 gcgatcctga gggcggccat cgtcgcctcc gtcccggtgc tgacgaacct gagcatatcg
     6781 atgctggggt agtggccgac gatccttttt gctagggaga cctccagctc cgagggggtg
     6841 ccgtagagcc agccttgctc cagctggcgg actacccgct ccatcaccag ggggtgcctg
     6901 tggccgagga tcatgggtcc gtaggcgagg cagtagtcga tgagcctttg gccttccttc
     6961 gtccagaggt gagggccctc ggccctggag acgtagaagg ggtaggggga tatagcccgg
     7021 acggggctgc tgacgccccc cggcatcagc cnnagataga agaaccttgc gattagatcg
     7081 gatcctcgta ataagatctg attatccctc atctgaaaga gtataacaat ccaggagatg
     7141 tcagggcctc gacggatggg ccgggaccgg gggccggccg agctcttccc aggccgcctc
     7201 ctccgccctc ctcgccacct ccctcagagg aaggcctgcc tctcgcgcga tccgccggca
     7261 gtcgtcgtgc t
//
LOCUS       DASM01000095           14092 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_14969, whole genome shotgun sequence.
ACCESSION   DASM01000095 DASM01000000
VERSION     DASM01000095.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 14092)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14092)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14092
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_14969"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2547 a         4598 c         4198 g         2749 t
ORIGIN      
        1 tatcctttgc ttcaaacaat ccaggaaaaa atatatacga tcagattaat attagcggaa
       61 ttgctcagac cctctggccc gatcattgca tacaggggac gaagggggct gaatttcatg
      121 acgacctaga tttaagtaag ttctgtctga tattgagaaa agggatgaat cccctggtcg
      181 attcatattc tgcattcatt gaaaatgaca gaaatactga aaccggtctt gctgggtatc
      241 tgaaggccct gaaggtacga gagatattca tatgcggtct cgccacagat tactgcgtct
      301 attactcagc catggattct cttcgatatg gattcagatg caatgtgata atcgacgcca
      361 cgcggggggt ggatctgccg gagggaagca tcggtgccgt cgtttcagag atgaaagata
      421 aaggaatatc ggtgataagc tccgatgatt tattatgata aattgaggag ttgaccccgc
      481 cctgccatga ttcaagctgc cctcgacgaa tggatactaa atgaggcgat cccgttctcc
      541 ctcgatccgc ccgagaccct caacggagcc atcgacaggc tcatcgccag cctcgatcca
      601 tcggtggaac tcctcggctt cggcgaggcg ctccacggcg gcgaggagat cctcctcctg
      661 cgaaaccggc tcttccagcg ccttgccgaa aagcacggct acagcgccat cgccatggag
      721 agcagctttc cccggtcgag ggcggtaaac gagcgcgtca gcggcagcgg cagcgccgaa
      781 tcctacgacg ccgtccggga ggtggggttc agccacggct tcggcagcct tgaggcgaat
      841 cgggagctgg tggagtggat gagggcgtac aacgccgatc ccgcacataa agcaaagctc
      901 catttttacg gcttcgacag cccgacggag atgactttca gcgacagtcc gagcggcgtc
      961 ctccatttca tcctcgacta cctcgaatcg atcgatggcc cacgcgtccg ggcgcgtcgc
     1021 gagcggatcg actcgtacct gggcgaagac ctcggctggg agaatcctgc cgccatgatg
     1081 gacccctcac agtcggtggg gctctcacat gaggcgacgg cgctccggat cgagacggag
     1141 gagctgatcg cagagctcgg cctccgccgt ccggagctcg cggccagggg cggcgtgagc
     1201 cgctaccagg aagcagtgca ttatgctaat gtagcaaggc agcttctgaa ctatcatgca
     1261 gtcctcgacc aaaattcccc cgaccggatc gtcctggccc tcggcctccg cgattctatg
     1321 atggcggaga accttgagta catcgtctcc cgtgaacgcg gccgaggaaa aggtgctgat
     1381 ctatgcccac aatagccacc tccggcgcag caaggtcgag tggcagctgg gctccgacca
     1441 cctccgatgg tggccggcgg gcgctcacct cgcggagatc ttcggccccc gctacgcgga
     1501 catcggaacg gccgtcggca tctccgagga gaacgggatc ggcgacctcg acgactttga
     1561 cgcgctggcc tttctgggcg atgtggtata cagccgggac gggccggtct tgccttagga
     1621 tggagcagta agaggggata ggcatttcga ggtgaaccct tgccgggaag gtcggtggga
     1681 ggcgaagttt taacataatt atacttcaga actcttcgcc atgaacctca aacagatcgg
     1741 cgtgatccac tcccccttca agaccagggc cgaggccccc ttccagggga ggcactcggt
     1801 ggaggtatgc gaggtggagg tcttcgagga gtttctgccg gggctcaccg acgtggaggg
     1861 ctgcacccac ctcttcctcc tctactggct cgaccgggcc gatagaaaga tgctcaaggc
     1921 cttcccgccc cacgacggca aagagcacgg cgtcttcgcg acgaggtcgc cgaaccggcc
     1981 aaaccccatc ggcctcgggg tcgtcgagct tctggagatc tccaggggga ggcttcgggt
     2041 ccgggggctc gacgccctgg aggggacgcc gctcctggac atcaagccct acttctccga
     2101 ggtcgacagc atccccgacg ccaccgtcgg ctggaggaag tgatgggatg gggcgagcgg
     2161 aagggggcga gcgaggggcg gagggcactc tggcgatggc tcaagggcac ctcccccccc
     2221 accccttcga ttccggccca gccccccgat ggggctacct cgtggaggtg gggcgggtgt
     2281 agcaggcgcc gtcggccccc aggatcatcg acatcggcct cgccctctca aagtccatcc
     2341 cgccttcggc gccgaagaat tcgtcgttcc cctccaggaa gacgaccctc ccgatgatca
     2401 gggagaacct ctcccggagg atctcctcga ccagctcgca ctccatccag gcgatgcaac
     2461 ctgcgatccc cgggggcgcg accttcgtcg agggatgggg ggacagaccc gcctcctcga
     2521 actcgtcgac ctccggcggg aagctcctgg cgcagatctc cacctcttct gccatatcga
     2581 gggtggggac gttgacgacg aactcccccg tctcccggat gttgtcgagg gtatccctct
     2641 tcagccagga ggcgatggcg atctcgtcga gaggcctcag gatcggcatg atgcaacccc
     2701 agggcgcagc gtttcggacc cctttcttcg agaccgtcga tatcaggacc accggcatcg
     2761 gcagaacctg ccctctcatg ctaggcttca gaatcatatg gaatcaccat gaatatctct
     2821 taggacaata aaaaagaata tccgcggccc ggggccgcgg agaccctcaa ttcggaccag
     2881 cctcgatcct acaccccgac gaaggagagc ttgttcaggg cgatgggtat ccccagagcc
     2941 atcccgttgt gggtgtagta gaggtcgacc tggccgtcgt gggcgacgaa ggattcggtg
     3001 tagaataggg tcagggccgg cacatcctcg gcgtagagga cctgagcctc gtcgatcatc
     3061 tccctccggg aggcctcatc ggtctccttc acctgctcct cgaggagctc cgtcagatcg
     3121 gggttgtcgc ggtaccgggc gctgttgaag tcgtcgtcga gggtgttacc ggcgaggaag
     3181 ttggggtcgc cgatgactcc gccgtggccg ctgacggcga ggtcgaagtc ccactcgccg
     3241 accttggagt cgacggcctt cgattccatc gtcctgaggt cgacgttgat cccgacggcc
     3301 gtaaggtccg cctcgatcag ctcgccgatc ctctcggcgg tggtgtcgcc gcccttgatc
     3361 agaacctcga tcttccggcc gtcgtagccc atatctgcca ggagctcctc ggcctcagcg
     3421 gggtcgtagg ggtagtagtc gtccatctcg gggttgtacc attcgttatc cgagggtatg
     3481 aacccggggc ttccggcgag gccgtagcct cggcctgcta tctccaccag cttctcccgg
     3541 tcgatggcat aggcgagggc ctgcctgaac ctcttgtccg agaggggctc cttctggtgg
     3601 tttatcatca gcttatagtt ccaccagtgg gcagggaccg agaggagctc aaagctcccc
     3661 tccagctggc cgatcatctc cggctcgatg gaggccatgt tgaccctccc ctggaggagc
     3721 tcggcggcgg cgttgggggc cgagatcttc acgaatacca gctcttcgac cttgggcgcg
     3781 ccgaggtagt agccgtcgta ggcgacgtac cggtacgtcc cctgggccat gttgtagtcc
     3841 tcaaccttca gggtgtaggg gccggtcccc accagagctt cctggtcccg gtagttgacg
     3901 gggtcgtcca cctccgacca gatgtgctcg gggaggatcg ggagggtccc tcccacctgg
     3961 tcgaggaagg gggcgtaggg tgccgagagg gtgagcttga cggtgaggtc gtcgagggcc
     4021 tccgccctct cgatgatcgc cgagtcgacc cactggtagg agtggtcttt ggtgtactcg
     4081 acggtgaaga cgacgtcgtc ggcgcggaac ggttcgccgt cgtgccaggt gacgccgtcc
     4141 ctcaggtgga agatgtagac gtcatctccc tccatctccc acctctcggc gagggccgga
     4201 acgtagccgg cgtcatcctt ccagaccagg gtgtcgaaga ggaagctcat ccgcgtatac
     4261 cccggccccc gggagtagtg ggcgtaaggg gagggaaatc cccagtctcc ggtggtgtcc
     4321 gccaccgtat atacgggaac tgacccgcca tcctcggagg cggcggctcc ccaggcaaaa
     4381 agcaggacca tcagagctgc agcaactccc agtctaatga aatctctcaa tctaggttcc
     4441 tccattccat ttctaggatt aatcttacga tatactgaat tattcttctt atttatgaat
     4501 ctttttgttt agactatatg taaaataggg tctagtaatc agaagttatt tattaattgg
     4561 acaaatggaa agaataaaaa tctaattggc atatctttct tttttgaatc atacatcaag
     4621 tgcttaatca gattattaaa tctgatgaat atatcaatgc cgcaggcagg aagatacctg
     4681 ggaggcgatc cattcaacct gatatgatgg gctttgtttg aggcgatcgt aagatatgga
     4741 tcgagcccaa cgatccatcc ggcagctctc gggacggaga atatcagctc agatctccaa
     4801 agggcgcccc gccctcagaa gtgattctta aataagatca gagggaaaca gaaaattaag
     4861 atgatgagca gatggcagag atgctgggat gtcgccccgg agatacacct cctcctcaaa
     4921 gggagcgata gcctggaggc ggcgagggac gtcgtcgtcg gctacctcca gtccctggac
     4981 tggacccacc ggcgggaggc ggccgagatg gacccctggg attacgtcct cttcaacgag
     5041 gggctcgagt gcctcaagaa cctcttctcc ccccggaacg agcgggcggc gaagacctcc
     5101 tccctggagc agctctggag ggccgccgcc accggcgacg ccgaggtctc cgacgacttc
     5161 gtcgacgagt tcttccacct cttcaaggcg gttaaggtcc agacggacgt ctacccctcg
     5221 atgctgatgg aggggatggc cgtccccgac ttcacgaagt acgaggggag ggaggcgggg
     5281 aagaggaggt ccgactacct cgacgagatg gcccgccgga tggacgagca cctctcgagg
     5341 tacagaagcg gcctcgaccc cgaggtcgcg agaacgaggg cggagaatag ggagaggatc
     5401 ctcgccctcc tcgggggaga cgaaaacgac tggctcgact ggcgatggca gttccgacac
     5461 gtcttcaagg acgggcttgg tctgagccaa ataaaagaga ttataaatct gagccccgat
     5521 cacgatagat cgatcggcct cgccctggag aagggggtcc ccttcggcgt caccccccac
     5581 tacctccacc tgatggacga agatccgacc gaccaggact tcgccctccg gcagcaggtc
     5641 ttccctcccc tgaagtacgt cgagaagatg atcgcccacc ggggggatcg aaaggtggcc
     5701 ctggacttca tgagggagcg ggacacctcc ccggaggagc tggtcacccg ccgctacccg
     5761 agggtggcga tcatcaagcc ctacgactcc tgcccccaga tctgcgtcta ctgccagcgg
     5821 aactgggaga tcacctcccc cttcatgccc tcggctatgg cctccgaggc ggagatcgac
     5881 ggagccctgg actggtatgc ggagcacgag gcgatgatgg acgtcctcct caccgggggc
     5941 gaccccctgg tgatggccga cggcctcatc gataaaatcc tcggccgcct cgcggagatg
     6001 gaccacatcc tgagcatcag gatcgccacc agaacccccg tcaccgtccc ccagaggatc
     6061 accgaggagc tctgcgagat cttcgcctcc taccacgacc tcggtagaag aagcctctcc
     6121 gtcgtcaccc acttcatgca cccccgcgag gtgacggcgg agacggtgga ggcgatcagg
     6181 aggatccggg tcctggggat ggacgtctac aaccagcagg tcttcacctt cgccaatagc
     6241 cggaggtttg agacggccgc cctccggata gtcctgaaga agatcggggt cgatccctac
     6301 tacaccttca acatgaaggg gaagagcgag atggaggagt acagcgtccc catcgcccgg
     6361 atcctccagg agcggaaaga ggaggcccgg ctcctcccgg ggatcttcag gaccgacgag
     6421 ccggtcttca acgtcccctt cctcggaaaa gaccacctcc gggcctggca gaaccacgag
     6481 atcataggga tccagcccga cggccgccgc acctaccgct tcctcccctg ggagaagaac
     6541 atcgtccagg tgagccccta catctacgac gacgtctcca tcggcggcta cctcaggagg
     6601 ctggaggaaa ggggggagga cccggagaag tacaggtcca tctggtacta ttactgaagg
     6661 gccatgccgg gatctgacga cacttctgct gaaggatggc ccactgccta tttgcccttc
     6721 gtccttcagg gtgaaaggcc cgactgctcc gccccagcca cctcgaccgt caccctcgcc
     6781 tcgtcctccc cgacggcatt ggctgcggat atagtgtaga ccgtcgtctc ctccggctgg
     6841 acggcgagcc tcccctcccg ggcgacgggg ccgatttcgg gctcgatcct gacctcgtcg
     6901 gcgaggctga cgccggagac cctccagcgg aggacggtgc cctcccccgg ccggaccgaa
     6961 tcggggaccg cctcgaagag ctcgatcctc gggggggctt cggccccatc ccccgccggg
     7021 gcgatgagcc ccaggtggtc gatggcgagg aggaggccgg tgaagatgac ggccatgacg
     7081 gcgatcccga agagaaacct cttccagtag cctccgacct gcccctcgtc cccttctcct
     7141 ccgctcggat cgccgtccgc cctccgccta gagaccatct cttcccctcc ctcctcctca
     7201 gcccctctcg aggtacttga tcagatccac gagccccagg gcgttgagga cgtcttttct
     7261 ctccagccag ccacgcctgg cggtcttgat cccgtactcc atcgccgaga ggccgcaggt
     7321 gctgtgggcg tcggtgttga tggcgagctt cacccccagc tccctggcgc ggcgggacca
     7381 ccggtcgttt aggtcgagcc tgctggggtg ggcgttgatc tccatcgccg tccccgtcct
     7441 cgccgccgcc tccaggaccc gttccaggtc cacctcgtag gcctccctct ccccgatctt
     7501 cctcccggtg gggtggccga tgacgtcgac gtgctcgctc tccatcgccg agatgatccg
     7561 gttcgtcatc tccctctccc gctgatggta gctggagtgg actgaggcga cgacgacatc
     7621 gcactccgag aggatctcgt cggggtagtc gagcttcccg tcggccctga tctccacctc
     7681 cgtccccacc aggatcgcaa acccctccgc cacctcgttg aggcttctga tcagctccat
     7741 cttctctcgg agcctgtctt cggcgaggcc gtttgctatc cccaggccgt gggagtggtc
     7801 gcacatcgcc atgtactcgt agccgaggcc ttgggctgcc ctcaccatct cctcgatgga
     7861 gtggcggccg tcggaccagt cgctgtggac gtgaagatct ccctttatat ccgacggctc
     7921 caccagccgg gggagcctcc cctccagggc cgcctccacc tccccctggt cctcccggat
     7981 ctcggggggg atccaatcca tcccgaggtg gtggtacaga tcctcctccc ggtcgaagaa
     8041 gagctcctcc ccgtcggcga gcctggtcag ggagtactcg gagagggagt accccttcga
     8101 tagggcgacg tgccggagct tgacgttgtg ctccttggag ccggtgaagt actggatcac
     8161 cgtcccgaag gacctggggt cgacgatccg caggtccacc tggatcgtct cctcgacgat
     8221 gacggaggcc ttcgtcggcc ccttcatcag gacctcctcc accacgggga tcgagacgaa
     8281 ggcccggatc gccgcctccg gagccgggga ggtcgccagg atgtcgatgt cccccaccgt
     8341 ctccctcccc ctccggaggg accccgccac gttgaggtct ccgatcccct ccaccttccg
     8401 gaggtgaccg aggatctcct gggcgagggg gagggccacc cccaggggta tcctgctgct
     8461 ccgcttcttc caccgctcga tccctcggag gatgttcttc tcggaggtcg ggcccatccg
     8521 ggggagccgc cggacccggt gctctctcgc cgccacctcc agctcggcga cggtcgagac
     8581 ccccagcttc tggtggagca tcgagacggt cttggggccg agccccgaga tctggaggag
     8641 ctcggcgagc cccggcggcg tctcttggag gaggcggtcc cgggcctcga tcctccccgt
     8701 ctccaggaac tccgagatct ttccggcgat ggctttgccg acgccgggga tcgcctcgag
     8761 ctcgcccctc ccggcgattg cctccaggtc ctccttcatc gactccacag tcctggccgc
     8821 cctccggtaa gccacgacct tgaagctgct ctcgccccgg agctccagaa gatcccccat
     8881 ctcccggagg aggcgggcaa cctcttcatt cctcacggga ggggggttgg tggaccatct
     8941 tataaagagg ttggcaggcc cccaaaaatg tccggagcgg gcctccgatc cggcggatag
     9001 cggcgtgggc accgacgggg ggatcgtcgt cagacgagat cctcgatgtg ggtcatctcc
     9061 tggatcttgg tgaggctgct tatcgactcg gtctcgatcc tgatcccgtg gctcatggcg
     9121 gcggcgacga tgttcgtccc ccccaccagg gagagcccca cgtggtcccg ctccaccgag
     9181 acgccgagga cgttcatgtt cggatctccc aggtccagga cccccgaaaa ccccgcctcc
     9241 gccagcagat cgagcctctc ctcgatcctc tccgccgcga tcatcggcgc ctcctggagg
     9301 tttccgagga tccggccgct gccggtctcc atcatctcca ccaccgatgt cagctcctgg
     9361 ctgatgagga cgtctatcgg atcgatcgtc gtcgcccagt acatcagcat gtccgtgaac
     9421 cggaccggct cgcgatcgat cacctcgatc acccctcccc cccgggggcg gatcggaacc
     9481 cccgccttca ggaggacgcc gctgagggtg atgctgcagg cggacatgat cttggccttc
     9541 gtccccaggg tctccacctt caggtagggg ctgacggcga ggccgctctc tatcacgtcc
     9601 cggaatatcc gcaaggtcgg gtccagcatc ttcaggtcga cgagggaggt gttggtgatg
     9661 atctccccct ccatcctcca ggggtcgaag gagacctgat gcatcaggtt ctcgattctg
     9721 ctcagggaaa agaccagggg cttcaaacct accaccgaac ccttcctgcc gcggtttctg
     9781 ccccgtcctt ctgggccttg cggcggtccg ataccccaag gtagctcatc ctttaaatac
     9841 ggtggagatg gacgggaaag gcgtcttata tcacctctta catacttggc caagatgttg
     9901 cgccagaggt ttgtcctcga taccaccgcc ctgaccgact ccctcgcctg ggagggggag
     9961 ggttgcacca acatttgcga aggcatgaac gccatcctcg actgcgtcgc cgaggcgagg
    10021 ctccacctcg ggataagctg ctacatcccc tacccctccg tctacaacga gatcagggag
    10081 ttcgtccacc acaacggctg cgacgaggcg gttttgatca aggtcgacac ctggctcgtc
    10141 aagatgaccc cggaccgcta cgaggtgaag atcccctcca aggtcttcta cgagtacgtc
    10201 tactacatga ggagcaggat caacaagggg atgaacgtcg ccgaggaggc gatctgggag
    10261 gcggtgggaa ggtgcatcgc catgagcgct gcggaggggg acatctcggc attcaaggac
    10321 gagatcgaga gggaggcggt gggggggatc gtgaggaagt ttcgggagaa gtaccggagc
    10381 gcgctccgct acgggatcct cgacagcgcc cccgacatcg acatcctcct cctggcgaag
    10441 gagctgaacg ccgcggtgat atcccaggac ctggggatcc agaggtgggc ggagcagctg
    10501 ggcctccggt acctggaggc gaggaccttc cccgtcatga tcaaggagta cctcagtcgg
    10561 gcgagggcga tccataagag cgacggcctc cccctctacg tcccgccctc gggggaggac
    10621 gactccctct agccgatttt attcaataaa attttggcat cgggccggtg aggaggaggt
    10681 gggacctgga tcctccccct cctcttcacg accccaggag cctctccagg agccactccg
    10741 gctcgaactt taccaggtcg gggtagtcct ggccgatccc gaggaatagg acgggctttc
    10801 cggtgatgga ggatatggat atcgccgccc cgcccttggc gtcggcgtcg gtcttggtga
    10861 ggatcgttcc gtcgatgggg accgcctcgt tgaagagcct cgccctctcg acggcgtcgt
    10921 ttccggcgat cgcctcgtcg acgaatatca aaaggtcggg gttcaccacc cggacgatct
    10981 tcttcatctg gtccatcagg ttgacgttgg tgtggagcct tccggcggtg tcagcgagga
    11041 cgatgtccct gtggtgggcc ttcgcgtact cgacggcgtc gaagatgacg gcggcgggat
    11101 ctttgccgtc cctgtgcttg accagcttca cctccagctt attcgcgtgg acctcgatct
    11161 gctcgaccgc cccggcccgg aaggtgtccc cggcggcgag gacgatggag tagccccggt
    11221 ctttgagata cctcgcgacc ttggcgatgg aggtggtctt gccggtaccg ttcaccccga
    11281 cgaagacgat cttgacgggc ttctctgcct tcgcgatgaa ctcgtcgacg tcgaagcggt
    11341 tctctgagag gacctggagt agggcagacc tgagggcctc ctcgacgatc tcgccggtgt
    11401 cggcccggat cttcttcttc tttccgacga gctccccctt gacggaggcg acgatcgtct
    11461 cggcgacggg gagggcgacg tcgctctcca ggagggccat ctccagctcc catagaggat
    11521 cctggagatc cttatcgtcg aggacgacct cctgctcgaa gatgagggat ttggccctgg
    11581 aggcgaagga ccacttcggc ttggaggcgt tattctcggg ggcaggtccg tcatcgactc
    11641 tatcttcgac tgcgacctct tccctctctg ggggagccct cctctcttcg gcctgggcct
    11701 tcgcctcggc cttctcggcc tgggcctccc ccttctcctg gatcttgctt gagaccgcct
    11761 ctttgaaacc cttcagcctc tccttgaacc tgttgaacaa atccttcgcc cctactgcga
    11821 gaactgcctc tcatactggg cggcgatcga ctggatcttc cccatctcag cctcgatcct
    11881 ggagagggcc tggttcagct tggcggtggc ctccgatagc tgcgccttcc tctgggtgag
    11941 ggcctcctta gcctcccggg tgctcttctc gatgctcacc ccggcgccga ccccgaccac
    12001 gaccttatcc ttggaggcga gcctggcgtg gacgaaggac ccctggccta tcggcaccag
    12061 aatctcctgg tcttccccgg cggcatccat agcctcgacg gcggcgaggg ccttctcgca
    12121 cccctccatc gagagctgga agatccccag ctgctggacg atcgcctcgg cctcggcctg
    12181 gtgctgctga tataccatca agagtctcct gatctcctcc tcgctgggag cagccggaga
    12241 ctcagccaag agccttcacc tcttctatcc tcaccaggtt tctcttgacc ccctgcttgc
    12301 ttcccaagag ggagtaggtc ttctccacgg cgttcttctc gctgttgctc tcgaccacct
    12361 gggagaagga ctgccagctg ttcctcacct tgaactttcc cttgatctcg aaatcgacca
    12421 taagactacc ccataaaacc caacgcatct tcgatacggc ccagctctgg ccccgtcgtc
    12481 tcgtccccgg cggcgtaccc ccggctgttg gccaggatcc ccgatcccac caggggcgag
    12541 ccgaagttca ccgtccccac gtccaccggt aggcggaaga ggtcctccac ctcggcgagc
    12601 tccgcctccg agaccctcgg atggacgagg atcccccggt tggtggcgac ggaggtcatc
    12661 ccgacggtct tgaggcctcc gatcgtcccc cggcggacct ccacccccag ggtcctcgag
    12721 atgctctcgc aggcccggtc cgagagcccc ggatgtacga gggcggcggt gtcgttggct
    12781 aggatgacgt tcccggcggc gctgatcttc cccgagagcc tggccacctt cccgaaggcg
    12841 gagaccctcg ccacctcctc atcgctggca tggggggtca ggatgaagcc ccggctgttc
    12901 ccacaggcca gggaacccac caccgagctt cccgccaacg tcccccgaaa gggggcgagc
    12961 cccagatgcc tctccagggc tgccatcgcc ccagggcccg tctcaggggc caccaaaagc
    13021 gccgtctcgg tgcagcgggc gaagaccccc agcagggcgc ttcccgccac cttcagccgt
    13081 ctatcggacc cgttcgcctg caaactcagc ctcaactcca ccgtcgtcga acttggccgc
    13141 cttcaccctc accctcgccg gcggtttctc tgagccccga gcccagatcg cctcgttgag
    13201 gtcgcgagag atcttgacct cctcgatgga ggtcttcatg tgctttgcga ggtactttcg
    13261 gacctcgtag accgccctct tgctcctctt ccacctcgga gccttcttca cttccctcaa
    13321 aggaatggta taaaccctct ctgtctccgc catatattcg tccctcactc tatgttgctc
    13381 cgtctccagt gacgtctctt ggggtggctc accacttttc tcatcgtctt tatgatcacc
    13441 cagccgggca ctcgcctgtt ctggcagaag gccttggcat gtctgatctt cttccccttg
    13501 gttctcttgc tcaaatccga atcctcctaa tcactctggt atgcatgtgg ttggcaggaa
    13561 atatcctcgc aacggcatcc tgcccgtgcc gctcctccag ctcccgccgc agacctacct
    13621 cgaagtcgga ggcggggtga aggccgcttt ccagggtcac aaactccatg tacctcttcg
    13681 ccagggcgtt gatggtcttg gatccgtatc ccgcgagggg ccttatgtag tggaccttcc
    13741 ggcggtcctc caggctccgg accccgtccg gggtcatcat cggaacccga tcttccctct
    13801 tcgtcccgtc ggcgatatgc tcccagcccc cggagaggac cgccagagcc tcccggtgga
    13861 ggcggttcag gccgtccctc ggatatccag tcgccaagag aaggccgatc gcctcctcca
    13921 ggaccccgtc ttcaaaggtg atcgttctgt gggggaggcc gagggcctcc gccgcctccc
    13981 tcgccgcctc ggcggtcccg tggcgacgga aggcgaaggt tgccagctcc acatcaaaga
    14041 acggttccag aagaatcgag gccagggcgc tgtccttgcc gccgctgaag ag
//
LOCUS       DASM01000096           10299 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_23287, whole genome shotgun sequence.
ACCESSION   DASM01000096 DASM01000000
VERSION     DASM01000096.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10299)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_23287"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1831 a         3365 c         3260 g         1843 t
ORIGIN      
        1 tcggcctcga taccctgagc atctatgacc tcgtcctaat atcggtattg agcagcatct
       61 tctccggcct gatcctcctg ggattcaccc tcctggtgat agagaagagc ttcaggaggg
      121 gttgggaccc cgacaacatc tccgtcccct tcatcaccgt cgctggggac gtgataacta
      181 tacccatcct cttcgggacg gccatcttcg tcacctccct cggccagccc ttcaccgggg
      241 ccgggaccct cctcttcctc ttcatctcta ccgcagccgc ggtttacagc atcggctcaa
      301 accatcagct gatggcgagg atagtgagac agtcgatgcc ggtcctcatg atcgcgaccc
      361 tcttcagcac cgtggcgggg ctcttcatgg gggtctacga ggagatgctc ctcgccttct
      421 cgacgatact cttgctgata ccggtcttca acgccgtcgg cgggaacctg ggggggatcc
      481 tctcctcccg gctcacctcg gcatatcatc tgggtctgat caagatggat gggaggctcg
      541 agggggagac gaagataaac ttcggctgca ccctcgtcct ctccgccttc ctctttccca
      601 ccctcggcct cctcgccttc ttcgtttcca gggcctccgg gatggacgcc ctcggcttcg
      661 gggacctcct gacgatctgc cttttggccg ggctcttcac caccctcctc gccatcttcg
      721 tcacctacta ctttacatac ttcttcgtca agatcggcgt cgatcccgac aacgtgacga
      781 tacccaccat cacgagcatg atggacgtcc tcgggacggc gtctctaatc ctcgtgacca
      841 tggccgtcgt ctcggcctga tgggtcccga ggtggaggag aaatggacaa gaaaggacaa
      901 aaaagggcaa ggagaggacg aggaacgggc aaggaaaggg gggtggaaag ggcgccggag
      961 ttggggagtc tcatcggctc gccatcaggt ctccgaccct ctccagggct ttcggtatct
     1021 gtcccctcat gatcgccttc atctggtcgg ggggaaggat gccgaagagg gcgtccatca
     1081 gccggtcgct cttcatcatc ccatcgacga cctcccggat cctgacggag ttggccagct
     1141 cccgccccat ctcctccgcg atccattccg ggtactcctg gagggcggcc ttgcccccga
     1201 tgtagcgagc cgccacctct cccgccgccc gcccccccac catcgccagg gggatgccgc
     1261 cgccggaggt ggccatcacc atccccgcgg cgtcccccgc caggaggacg ttccccctct
     1321 ggatcgcggg gggcatcccc ccggcgggga cgagcccccg catcaccgcc gtgacggacc
     1381 cctcccggag cttctctttc gctaccgggt gctctgagat gaaggagtcg aggacttcgt
     1441 gaagcccccg cccccgaagg taggggggcc tcgtcccgac ccccacgttc gcgacgtcct
     1501 ctcccagggg tatgatccag gcgtaccctc cgggggcgga cgccgtcccg aagtacatct
     1561 cggcggcggc agggtcgata tccacccccg ccatctcgta ctcgagacag acccccacct
     1621 cccccctcga ggccaacccc gcgccccggg cggtggcgga gccggggccg tcggctccga
     1681 tcaccacctt cgccttcacc gttccggagg ccctacccga gaggtggagc ctcccccccc
     1741 taagctccgc cctgacgccg acgaggaccc gggctccggc cctcgccgcc cgccacgcca
     1801 ggtggcgatc gaaggacctc ctgtcgatca ccctcccgag gacggggaac tccttcgacc
     1861 tccccgaggg ggagcagatc ctctggaccc gggtccggtg gaggacgagc ccctcgggga
     1921 ggtcggccag ggcctcgggg atctttgctt tcggtaggag gccctccagc tcctctatct
     1981 cggggaggaa gccgccgcac tggactgggg atcctatctc cctcttcctc tcgatcagga
     2041 cgacctcggc ccccagccgc gccgccgtct ccgcggcgac ggagcccgcc ggccccgccc
     2101 ccaccaccgc cacctcgacc tcatccaggc cgccagccat ctccatcacc cgacgggggt
     2161 cgccgagagg ccgagctcca ggagggtctt ctggatctcc tggtaagaga ggtagatctc
     2221 gtccttctgc ctctcgatca tctccctctt cggctccgcc gagagccaga tcgagagccg
     2281 gtctccccgg accatcttca ccgaggcgag catgtccggc tgggtgagcc tgtagatggt
     2341 gtccttgccg gtgagctcat gccatgcctg gtgctctgcc tccagccgcc ccagaaactc
     2401 gctccaatcg agcctctcct cgatcctgcc gccgtcggcg aggatcggcg cgaggtctag
     2461 gcggaggtgg aggtgggccc tctcccccga gaccttctcc tcgatcttct tagcgatctt
     2521 tccgtactcc ggatcggtcc ggtcggcggc gagggctccg tcgccgtaga cctcccaccc
     2581 gacgtcctcg aagaacggcg caagcctcgc cgcctcgggc ctcgccgtcg cgagagagac
     2641 cccgacgaag acgagggcgc tgagggagag gcctatgagg acgccgtgct ctgccaggat
     2701 gccgcttgcg gcgatatcgg ggaacgccgc ctccaggggc gccttcagga cgagcttctc
     2761 gtaggatatg aggcctatct gggttcctcc accgacgatc accgccgcca gggcccccca
     2821 tcgagtcgcc cgcttccaga agaggccgcc gatgatcgga aagaagtagg aggtggtccc
     2881 gatcaccgtc gcgatgatca ccgcctcgac gatgctctcg acgtggaggg ccacggctgc
     2941 ggcgatggcg atcatgacga tgacgagggc cctgttgatc accagcatct ccttcatgct
     3001 cgcctggggc ctgatgtgcc tctggacgat gtcccgggag atgcaggagg caccggaggt
     3061 ggcgaaggtg tcggcgcatg acatggaggc ggcggcaaac cctatagcga agagggcgac
     3121 gaggccgccg gggaggctct cctggatgaa ggtgaggtag agcatctcgg cctcgatctc
     3181 gccggcgggg aggggataga tcccccggag ggcgacggcg gcgacgaggc aggcgaggta
     3241 gacgaccgcg agaaggatgg cccccaggat gaggccgcgc ctggctgccc tatcgtcccg
     3301 ggaggcccag atcttctgcc aggggtcctg ctcggcgacc catccgggga ggagggctag
     3361 ctggaggacg agggctccgg tgaccccccc caggatgaag aggttccacc agtcggcccc
     3421 cagggaggcg gaggcggcgg tgagggtgat cccccccgcc accgacgccc tcgtcgcgag
     3481 gatggcaacg cctacggcga cccccgccag gaataggaac tggacggcgt cggtccagac
     3541 gacggcccgg aagccgccga ggctgacgta tatcgatacc gcgacggcca tcagcaggac
     3601 cgcgtataga ggcggaaccc cgaagaaggt cgcgaggacg agcttgaacc ctatgaagtc
     3661 ggtcaccgcg aagaggacca tggtgatggc tatcgccacc gccaccgggg cccggacgat
     3721 ggggtggtac ctgatctcca gaagctccgg ctgggtcatg gcggggatcc tcctgaccct
     3781 cttcgcaatc cctgcgatga tcagaagccc cgcgacgttg gggaagaccc agacccagat
     3841 cgacccaacc cctatgtaaa gaaagaggcc ggtcgccagg aggagggcgg acgccgtcag
     3901 ccacgaggct gcggccgata ggccgatgtt ggcagccccg atctcgcgcc ccgccagcca
     3961 gaagtcctcg acggacctct gccctcggga cgaccggaag ccgatcgcga tgagggctgc
     4021 tatgtacagc cccagaagga ggaggaagag gtggtaggaa tccattctgt cacccctcct
     4081 ccagagggat cgcgaatata taagggatga gatctccgat ttttctagcc ggattacccc
     4141 ccccgaatct catctctctg ggccacggcc gaaggcacct cctcttggag gaggatggtc
     4201 tcgccccctt accgcccctc ggcccgcgga agacttaagg atgcggcgct caagtctctc
     4261 ccgaggttct tctcatgaga agtgatatca ccaaggtcgg gccggagagg gcgccccacc
     4321 gggccctcct caagggcacc ggcctcatag acgaggagat ggaccgcccc ttcgtcgcgg
     4381 tcgtcaactc ctggaacgag ttcataccgg gccacatcca cctcgacaag atagcccagg
     4441 cggtcaaggc cgggatcagg atggcggggg cggtcccctt cgagtttcac accataggcc
     4501 tctgcgacgg catcgccatg ggccaccggg ggatgaagta ctccctcccc agccgggaga
     4561 tcatcgagga caccatcgag gtgatggtgg aggcccacca gctcgacggg atggtcctca
     4621 tctcctcctg cgacaagatc gtccctggcc atctgatggc ggctgggagg ctcgaccttc
     4681 cggcgatagc cgtcaccggc ggccccatgt accccggatt cgcctgcgat caagagctgg
     4741 acctgatcga cgtcttcgag gggtggcaga aggggggcga ggtgctggag gtgctggaga
     4801 acctggcctg tccgggggcc ggctcctgtg ccggcctctt caccgcgaac accatggcct
     4861 gcctgacgga gaccctcggc ctgagcctcc ccggctgcgc gaccgcccac gccgcagatg
     4921 ccaagaagat gaggatagcg aaaaaatcgg ggctcaagat cgtcgagctg atcgagaggg
     4981 ggatcaccgc aaggaagatc gtgacccgaa ggtcgatcga gaacgccatc cggatggaca
     5041 tggccatcgg cgggtccacc aacaccgccc tccacatacc ggcggtcgcc tccgagtttg
     5101 aaatcgatat cgatcttgag gacttcgatc ggttgagccg ggagacccct cacctcgtaa
     5161 acctccgccc cgggggaccc caccacatcc tcgacctgga gcgggccggg gggatccccg
     5221 ccgtcatgaa gcggcttcag tcgaagctcc accgggacgt cctgacggtg acggggagga
     5281 ccctcggcga ggagattgcg gccttcaccc ccgcaaaccc cgagaccttc gcgaaggtcg
     5341 tcaggaccct ggagagcccg gtccaccccg agggcggcat cgccatcctg aaggggtccc
     5401 tcgcccctga ggggtcggtc gtcaagcaga cagcggtctc ccaggagatg agaaagcaca
     5461 ccggcaaagc caaggtctac gactctgagg aggaggccct ggcggggatc gtcggcggcg
     5521 aggtagtcgc aggaaacgtc gtcgtcatca ggtacgaggg gcccaagggc ggccccggga
     5581 tgagggagac gctatcgccg acctcggcga tccaggggat cggcctcgga aactccgtcg
     5641 ccctgatcac cgacggcagg ttctccggcg gtactagagg tccctgcatc ggccacgtat
     5701 gccccgaagc ggccgtcgga ggtccgatcg ccctcgttga agacggcgac gagatcagaa
     5761 tcgacatcga cgcccggagg atcgaccttc tggtcgacgc cgggaccatc gaggggagga
     5821 gggctgcctg gaggccaccg gagccgaagg tggcgaaggg gatgctggcg aggtacagca
     5881 ggtccgtcac ctccgcgagc cggggcggag ttctcaggta ggtgagagat ggctcctaag
     5941 ggcgcgaggc ctcaggaggc ggggaactcc ccgaggggga tccggtcttt cgtcgccgtc
     6001 gacctccccg gaccgatccg cgacggaatg gcccgggtcc aggcggagat cgccctcccc
     6061 ggggtgaggc tcgtcaaccc cggcctcatc cacatcaccc tcaagttcct gggggacgtc
     6121 ccaccgtcga aggtggggaa ggtggccgag gccctccgaa gggtgaggcg cccccccttc
     6181 accgcccggg tgcggggcgt gggggccttc cccggccggt ccatccgggt cgtctggatc
     6241 ggcgccgagg gcgagttcga ggggctcttc tccgccgtag aggaggctct ggtccccctc
     6301 ggctttcccc gggaggggcg gaggttcaca tcccacgcca ccatcgcccg ggtcgcgagg
     6361 cccggccccg agacgaccgc cctcctcgcc gagaggctcg cccccttcag ggagatcgac
     6421 ctcggcgagt tccgagtcga ggagttttcc ctcaagaaga gcaccctcac ccccggaggg
     6481 cccatctacg aggatctcga atccttccct ctctcctctg actgaggggg tcctcgccag
     6541 ggtgaggccc tcctccgagg agcgtgagag gctggcggcc gtcgcgggga ggatcatatc
     6601 ccggatcgag gagctgatga gggcctccgg gatcgggggc gaggcggtcc tggtggggtc
     6661 cgcggcccgg gggacctggc tctcagggga ccacgacctc gacgtcttca tcggcgtccc
     6721 cgaggggtcc tccctcctcc cggccctgga ggtggcgagg ctcgtcgccc cgaggttcga
     6781 ggagcggtac gccgagcacg cctacgtcca cgccctcttc gaggggtttg aggtggatat
     6841 cgtgccgtgc tatctggtgg aggacgcctc agagatcata tccgctgtcg acaggacccc
     6901 cttccacagc cggtacgtct cggcgaggat acgggggctg gaggacgagg tcctcctctt
     6961 gaagcagttc atgaaggggg tcggggtcta cgggtcggag ctgagggtcg gcggcttctc
     7021 cggatacctg gcggagcttc tggtgatccg gtacggctcg ttcgtcggcg tcctggaggg
     7081 gtcctccctc tggcggccgg gggaggtggt cgatctggag gaccattcca gccggtccca
     7141 tggggatcct ctggtggtcg tcgaccccgt cgaccccgga aggaacgtcg ccgccgccct
     7201 caccctagac aggatgttcg ccttcgccgc cgcgtcccgt ctatttctga gggagccctc
     7261 cctggagttc ttcttcccgc cggaccttcc gcccctcccc gacgaagagc tccgcgaggc
     7321 cctcgcggag cggaggagca ccctcatcct cctggagttc gccgccccgg acctggtgga
     7381 ggacgtggtc ttcccgcagc tgagaaaggc ggagcagtcg gtccgcgctc tcctggagag
     7441 ggggggcttc tacctcctca ggtccgacgc cgactggcac cgggccgtcc cggttcgcga
     7501 tgacggggcg gaggggcccg gtccgtccga tgacgtcgga gtacttcgga tccttatcga
     7561 gctggaggtc gccctcctcc cccgggtcga gaggagggtc ggccctccgg cctgggaggc
     7621 ggagcacgcg aagaggttcg tcgcaagcca ccccgcccct ctatcggggc cttacatcga
     7681 gggcgggagg gtggtggtgg aggtggcccg gaagttcctc agagccgagg acctcctccg
     7741 gtcccaggtg gcgggcctct ccttggggcg ccacctcggc ccctccctga ggcgtggcta
     7801 caagatatac gtcggccagg agatcctgga ggtgagggac cccgagtttc ggatcttcat
     7861 ggccaggtac ttctcggcgc gagagaaggt cggctgatga ccatccgccc tcatcggaag
     7921 cagttccagt aggggctcat cgccctcttc acctcttcta tcctctccgc cgccctcggg
     7981 tcctcctcgc cgtcgatctg gcccgggtcc tcccccgaga agcccctcag gacggcgacg
     8041 atcgactcgg cgagggcggg gaggttgtag cccccttcga gggcggcgac gagcctgccg
     8101 ccgcagcatc ggtcggcgac ctccttcacc gccgtcgcca tgtatccgaa ggccccggag
     8161 gtgaggttca tgccgctgag ggggtctgcc tggtgggggt cctgccccgc tgagacgagg
     8221 acgatgtcgg ggtcgaactc cagcgccacc ggctttaaga tctcctcgaa ggcggcgaag
     8281 tagcccgagt cccccgtccc ccaagggagg gggacgttca cggtgaaccc ctcggcgccg
     8341 tccatcccca gctcttttac ccggcctgtg ccgggatagt gggggaactg gtgggtggag
     8401 aagtacaaga ccgacctgtc gtcgtagaag atggcgctcg tcccgttccc gtggtggacg
     8461 tcccagtcta cgatcaggac cctctccatc cccatccact gggcgtgcct cgcccctatc
     8521 gcgacgttgt tgaatatgca gaaccccatc ccgcggttgg ggagggcgtg atgccctggc
     8581 ggcctcacca gggcgaaggc gttctccagg ccgtcgttca ccctttctat gccggtgatc
     8641 accccccccg ccgccatgag ggccgcctca taggatcggc gggagacgac ggtgtccagg
     8701 tccaggtatc ctcctcctct ctcggagatc gtcctgacct gatctatgta tccgggggag
     8761 tggaccgctc tcacctcctc cagggtggcg ggcctcgggg tgacgagctc cagctccagc
     8821 cccgaggatc ttatcatctc gagggcggag atgagccgct ccttcctctc gggatggccg
     8881 tcggtctcgt gctccaggta gatggggtgg tatataaggc cagttcgcgc catagagcca
     8941 cctttgtcgt ctcggacgta aaaacaattc tccccgatct taaaggggcg tggggtttct
     9001 gaagctgaaa taatgggctg atttccattg ttgttcgatt attacgggat attatcacga
     9061 ataagactat aatggtgtat tatgtgctta aaaagtacaa aatataactt ttttacggca
     9121 aataagcttg ataataatgt tataatgaaa aatcaggcct tatgacagct ttttgtttag
     9181 ttttttccat ctcgcccgtt aatacggcca ataatccctc ggaactgcgg cataagtgta
     9241 ttttatattt ttctctggca ttaatcttgg agagtcatcg ggtcccctac cggccatctc
     9301 tatataaacc gccgacctac gaatcagaat ttcaggtgca gggagatggg gtctcgctgg
     9361 agagaccaca aaagatatat ggtgatatcc tctatcatat ccccatagtc ccaaagggat
     9421 cgggagggtg atgcagctat acagtgtcat attgatgcca aacagcaact gatggcgctc
     9481 tttggtcgcg ccacagaggc ggggagatca gtgtgggcaa tagttttaag aatcatcata
     9541 ctgtatgctg tatgaacctt gccccatagg ggatatgtag gaggttatcg aaataatgac
     9601 gaaagtgacc gcgattacgc ttaccgccct tgtattgctg ctagcgtcag ctatggcagt
     9661 aagcgcagtc gataaggtgg agatccgcgg tcctgtggcc gatgtggtcg acactgtgga
     9721 gccctacgtc tggggtccgc aggagtttgc gggtttctac tacgacatcg acgatgatct
     9781 gggcagagag tccatcacca tgaccatcac cgacggtaac gttctcgatg aggacaccgg
     9841 agtgatctac agcactgaag cccagcagaa tgacttcgac tatgaagagt ggggcaagta
     9901 ctggaccatc ggctttttgg ctgaggagta cttcgccgcc tacgtcgagg gaaccgctga
     9961 gacggctttc ctcttcgacg attcgaccga caccaacctg atggctgacg agcagctcag
    10021 caaggttttg acggacgacg acgaggagag aaccttcacc agcggcacgc ctctgaagct
    10081 cgccgaggac tacgagcttg ccatccaggc gatcgacctc gacggcaaca aggtctacgt
    10141 ccagctgatg aagaacggcg cagtcgtcga ctcctctgtg attgagccct ccaagaccgg
    10201 tgcaaccgtc aaggacaaga cttataccta caagaaggac ttgggcgaca ccgagaagat
    10261 cgtcgtcatc gctgtccact tcaagaacgc cttccgcgg
//
LOCUS       DASM01000097            4392 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_170449, whole genome shotgun sequence.
ACCESSION   DASM01000097 DASM01000000
VERSION     DASM01000097.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_170449"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          861 a         1370 c         1403 g          758 t
ORIGIN      
        1 actacgaggt ggcgatcagg ttcacaaagg agttctacga ggagaaccgg gagttcgtcg
       61 aggggctcgt cgggatcgta aacaagcccg tcctcatcga gatgtgggag gagcggttct
      121 tctacttcgt cctcaagttc gagttcaact tcgtcgacgc cctcgacaag gcctccgccc
      181 tctcgacggt ccagatcgac gtcgagaacg ccgaccggta cgagatcgac tacaccgatc
      241 ccgagggcgc cctccggcgg ccgatcatcc tccactgctc cccctccgga gccatcgagc
      301 ggctgatgta cgccctcctg gagaaggccc atcgggtctc caccgagggg aaggtcccga
      361 tgctggcgac ctggctatcg ccgacccagg tgagggtgat ccccgtggcg gagcgccacc
      421 tggcccgggc cgaggaggtc gctggggcga taggcttcag gacggacgtc gacgaccggg
      481 acgagaccgt cggaaagaag atccgggacg cgggccggga gtgggtgccc tacgtcgtcg
      541 tcatcgggga ctcggagctg gagtcaggga agctttcggt caccatccgg gccgattcgg
      601 agccgaagcg gcccgccaag gtcgagatgg ccgtcgatga actcagggcg aggattgcgg
      661 ccgagacggc gggaaagccc accaagaggc tacccctccc ggtgaagctc tcggagaggc
      721 cccggttcat ctgaagggtg gtggggccct gcccccctcc tctgaaggaa gagggggcct
      781 cggccggggg gctctttaga aacccaccgc aaagcctatt atcatccagc atatttcggg
      841 cgatctatgg caaatctgaa cgtggcgatc ctcggtcccc agggctacgc caaagaggtc
      901 gggaagaaag gaacctccac cgacatcacc ttctacaacc tgaagcgggg cgagagcacc
      961 gtcaccatcg tcgagccgac gcgctacccc gaaaggctcg cccccctctt ctttgcgaca
     1021 tccctcgcga ggaaggcggt cctagtcgtc gatgcgatcg acgccgcctt cggcgagggc
     1081 gtaatcatgc tccaggccct cgggattagg gaggggtaca tcatcctcag aaactacctc
     1141 gacccgggga gggtcaaccc cgtcatcaag gggacggtcc tcgcggggta cgagcagatc
     1201 gatgacgacc ccatcgccct ccgggagagg ctcctcgacg aggcggacaa gctccaggag
     1261 gctaccccgt ccccggagga gcgggacggc gcggtccccg tcgaccactt cttcaacgtc
     1321 cgagggattg gaacagtcat cctcgggacg atggccgagg gggtcataag aaagcatgat
     1381 gccctcaggg tcctccctgg cgagaagatc gtacaggtcc ggtcgatcca gaagcacgac
     1441 gacgacttcg actgggcccg ggccggggac agggcgggga tcgccctcaa gaacatagag
     1501 gccgatgagc tggaccgggg agacgtcctc gccagcgacg aggccctcag atcggaatca
     1561 agcctatccg ccagggcgga gctgatccgc tactggcccg cccccctcaa ggaggggatg
     1621 gtccttcaca tcggccactg gatgcagttc ctcccggcga gggtcgcctc tgtcgaggcg
     1681 ggggaggact ggaggaggcc ccttctgaag ctggagctgg agaagcccct ggtataccgg
     1741 cccggcgacc gggccgtaat ctgcccgctg gaggggagca agctccggat cgccgggacc
     1801 ctggagcttc cttaaaggcg gctcactcca ccaggacagc ggtcccgctg gcggagacca
     1861 tcagcatccc gccgttctgg ccgagggtct catagtccag gtctacggag aggacggcat
     1921 tggcgccgag cctcgccgcc tcctcctcca tctccgagag ggcgatctcc ttcgctcgcc
     1981 gtagctcctt ttcgtaagcc ccggaccttc cgccgacgat gtccctgatc ccggcgaaga
     2041 ggtccttgaa gatgttggcg ccgaggatcg cctcgccgct gacgatgccg aagtacttga
     2101 caactcgctt gccatctacg ctgggggtgg ttgatatcaa catgacatcc cttgacgact
     2161 tcgttgttaa tcatcctgac gatggaggcc gggatcatcc ccaatcgagg gctctatcga
     2221 ggtatggtct aaaaagggct gaaggggggg agggatcggc ccctcccctc agttctgccg
     2281 gagcccctct tgccagcgga ggagcctctc ctggacgacg tacctgaagg tccgatcgat
     2341 gaggaggccc aaaaggccga ggacgatcat gtagaggaat accagatcca tcatgtggag
     2401 ggagtagtag tcccagagcc ggtagccgag gccgctcctg gtggaagccc cgaacatctc
     2461 ggcggcgacg atgcacatcc atgcgatccc catggcgatc ctgatgccgg ctgctatcga
     2521 cggcagggcc gaagggatgg cgacgtacct gatgaggtcc cggttccggg tagcccccag
     2581 gaccatcgcc gactcgacgt agatcctggg gacggacctg aagccgacgt aggagttgat
     2641 caggatcggg aagaccgccc ccacgaagac tatgaacccc gccgcctggg gcgtcaggcc
     2701 gaaccagatg atggcaaagg ggatccaggc gaggggtggg atcggccgga tcagctcgac
     2761 gatggggtcg aagatccggt cggctacccg ggaccatccc atggccatgc ctatggggag
     2821 agccaccaga agcccggccg ccatccccat ggagaagtgg atgaggctca cccggaggtc
     2881 gacccagagg atcgtctccc actggcggag gagggcgtcg actacctgga ggaagctcgg
     2941 gagcttgagg gggtttgcta tggcggcggc caaaagctgc cagacgacga tgagccccat
     3001 caccgatagg gcttcaaccc cgtgatctgc gatcccatcc ctcaccctct caacgagggg
     3061 cgagtccgat ctatccacat cccctctcct accaggtgcc aatcgtccca tcactcctcc
     3121 tcagctcgcc acccggtcgt agaaggtggt gtcgaagagg tcgtccacac cgaggagctt
     3181 gcccccggtg tagcccagct cgtagttgac ccgggcaaac tccgtcaccg acgggatgat
     3241 ctggtgagga tcgctgatcc aggctccgtc ccagctcctg atggagtgct ctatcaccgt
     3301 caggttctga ttggtcttgg cggtgtatat cgcagcagcc ctctcggggt tctcgttgat
     3361 gaactccgta gccctgatgt gggttttgat gatctgctcc accagctccg gctcctccct
     3421 catcaatttg ccgctgacga cgaggcaaca gcaggcgtgg tcgggccaca tctctcccga
     3481 ggcaacgacg gctttgccat tgccggccag ctcgatgatg gagggcgagg ggtgggggag
     3541 gaagacggcg tcgacccttt tagccgagat ggcggtgacg gcagctccgg ggtccatcga
     3601 cttgatgtcg agatcggcag ggtcgacccc gttatccagg agccattttc taaagacggt
     3661 atcctggatc gacccagggg ggaaggtggc gaccgtccga ccgaccaggg acttcggacc
     3721 atcgtacgcc acctcaggcc tcaccaccag gtcagacccg ttcttctgga cggcggcgac
     3781 gatcttggcg tcgagcccct gggctatggc gctgatgggc ggggcggcgc cgacgtaggc
     3841 tatgtccagg cttcctccga gcatcgcctg catctcagga gggcctgagg ggaactcgaa
     3901 gtccttcacc tcctcgatgc cgaagggctt caggtccgcc agccaccacc cctcctccat
     3961 cgccaccatc tcggcgacct gatgggtgct gggctggtag ccgatccgca aagtcgtaat
     4021 ctctgcctta tccgaatcgg tgcctaggca gccgagggcg atgagggcga cgaggcccac
     4081 cgccagggct gcgagaataa gatctctcct caaaggtcat caccagagga gggaggaggg
     4141 ggtcaatata tagcattttc cggtatggcc gatatttcat cccaaaagta ctaatattta
     4201 gaaattattc tacaattaga tgacatacgc cgagaagttg atgagggcgg ccttcgcctc
     4261 cgacgagaag ctccgggagg ctatgaagga ggtcctggcg gagctggggc tctccgcccg
     4321 ggagttcagc gccgtctcct cgatacccca gtccaccctc tacaagatca tgtccggcca
     4381 ccgggagccg aa
//
LOCUS       DASM01000098            3653 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_231983, whole genome shotgun sequence.
ACCESSION   DASM01000098 DASM01000000
VERSION     DASM01000098.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_231983"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          692 a         1079 c         1190 g          690 t
ORIGIN      
        1 ctggagaccg gcggctggga gcggtactgg taagggtcac ttctctccga agtatatatt
       61 ttcgtcgtcg gtgcccagct gagagaactt ccccgtagcc aggatcttgt aggtgtactt
      121 gaggggggcc accctcgtcc ccggccccac caccgactcc tggggtagga tcacgttctc
      181 tcccaccacc gggatcttcc cggtcctgtt gaagggctgg tcggcgtcga ggacgcattt
      241 ggtcccgatg gtggagtgcc tcccgacgat cgcccggttc aggtacgacc ctctctttat
      301 cgaggagaag ctcatcacca tgctcccttt tatcaccgac cccctctcga tgaggctcgt
      361 atgaccgatg tgggagtcct cgatgaccac ccccttctcg atgctgcagt tcctaccgat
      421 gaagaccttc ccccggagct cgatatcccc ggagtcgagg tatcttccta tcctcttcag
      481 ggtcttgggg ttgatccact ggccggggcg gtactcgttc ctgaaggcgt agtgctccac
      541 ctttccgccg agaaagtcca tcgctgccga aagaagccgt tctggggtgc cgatgtcgat
      601 ccagtatccc ttcaccggat agccgtagac cggatatccg tgctccgtca ggtagggtat
      661 gaggtcacca ccgatgtccc gggcggagtc ccccatatcg gcgaggacct tccggatctc
      721 tggggagaag atgtaaaagg cggtgttgat catcctgctg ggctccttgc cgggggcggg
      781 cttctcgacg aagcctctga tcctcaggtc gtcgtcgatc ctggcgacgc cgtactggga
      841 tacctcctcc cctccatcca gctccttcag ggccacggtg aggatcgccc ccttatcccg
      901 gtgaaaatcg atgaagtcct tgaggttgat gtcgatgacg ttgtcgccgg agaccaccag
      961 catgtcctcg tcgatatcga agtagttcgc gcagtatctc agggagtcgg cgctgccctt
     1021 atctgcgtac tggggctggt aggcgaactc ctcgatatcg ctgatcccca gcctcttgaa
     1081 gaacccctcc cccgccttga agtagttgtt cagggggagg gtgttctcgg cgcccttgct
     1141 ccccaaaacg aacctcctgc atccctggat cgccaggacc ctgaagaggg tggcgatgat
     1201 ggcggtgtcg cagaggtcca ccaggggctt cggctggtcg agggtgaggg gatagagcct
     1261 ggtgccccct ccgccgacgg tcgcgattac cggtgggaac atcgcttcct catctccggt
     1321 cagtaaccgc gcctgatttt gattattatc cgggttatcg cgaagactat caggaatatt
     1381 atgacgtaga atattatctc ctgggtttcc atgtccatgc ctgggatctt gaagtttata
     1441 tttttgatgg gaggagatcc gccggaagag gagtgggtta gttatagaaa aagggggtat
     1501 atgaggactc tagcttcaga tgagggatgg atttgatgac cgaaaggaga gaggctattg
     1561 agagggcgaa ggcccacttc gaggagctcc tcgtcgagca gatggaccgg gtggagcgga
     1621 tgaaggccgc cgaggactgg atcgactact ctatgatcag acccatcgtc atcggcgttg
     1681 tggggggaga cggcatcgga ccctacatct gcgccgaggc gaagagggtt ctggagcaca
     1741 tgctccaggc cgaggtctcg gccgggaagg tggagatcag ggacatctcc ggcctcacca
     1801 tcgaggagag ggcgagggcg atgaaggcga tccccgccgg ggtcctcgac gagctgaaga
     1861 ggtgccatgc catcctcaag gggcccctga cgacccccaa gaagggggac ccctggccga
     1921 nngagctgga cctcttcgcc aacgtccggc ccgtcaaggt cccgagccag gggatcgact
     1981 ggatcttctt cagggagaac accgaggggg cctacattct cggaagcaag ggcttaaacg
     2041 tcaccgacga gatcgccttc gacttcacgg tgacgaccac ccagggggcg gagaggatag
     2101 tccagctcgc cttcgaccac gcgaagaaga acggggtcga tcgggtcacc gccgtcacca
     2161 aggctaacgt catcaagacc agcgacggca agttcctgga ggtggcgagg aaggtggcaa
     2221 aggactaccc cgaaatcgac ctcgacgagt ggttcatcga catcatggcg gcaaagctcg
     2281 tcgacccgaa gcggaggacc cagttccggg tcgtcgtcct tccgaacctc tacggcgaca
     2341 tcatcaccga cgaggcggcg gagttccagg gcggcgtcgg aaccgccggc tccgccaaca
     2401 tcgggaagag gtactccatg ttcgaggcga tccacggctc tgctccgagg atggtggagg
     2461 aggggagggc ccagtacgcc gatccctcca gcatgatgag ggctgcggcg atgctcctcg
     2521 gccacatcgg cttcgtcgag aaggcgaggc ggctggagat ggccctcgac ctctgcggcc
     2581 agtacgagag gaaggtcgtc ctgacgggcc gggccgacgg cgccactggg gccaagttcg
     2641 ccgactacgt catggagacc ctccaagatc ctgagctgga ggcgaggtgg aggagccatc
     2701 agtaggaaaa gctccacgca aaagatcggg agagccgctg tcccgggccc ggggagtcgg
     2761 cctcccctcc cttttcatcc gatgccgggc agatcttctg cctcctcctc tgatctatcg
     2821 agatggagga aaccaatttc tattataaac gacatatagc cctccgtgga gaacctggca
     2881 gatgctttcc tgagactgga ccagaaagag atcgccctcc tgaaggcggt tgagcggggg
     2941 atgcgcacct cggagtgggc ggcgatggag gatgtggtgg cgagagcccg cctctccccc
     3001 aaggccgccg cctcggggct cgcgaggctg gtgggaaaga agctcgtatc gaggaccacc
     3061 gagccctatg aggggtacca gatcggcttt gtcgcctacg acctcgtcgc cctctccgac
     3121 ctcgtcgacc gggacgtcgt cctctccttg ggcgacctcc tcggggtcgg gaaggagtcg
     3181 gtcgtcttcg aggccctcgg ccggatcccc cccggggcgg atgaggaggg agccccgccg
     3241 gagggggagg aggcggagct cgtccccctg gcggtcaagt tccaccgcca ggggaggacg
     3301 agcttcaagc acgtccgcag ggcgagggaa cacctcgccg accatccccg gtgcgcctgg
     3361 atccacgctg cgaggctcgg ggcggcgagg gagtggagga ccctcaaggc cctccatccg
     3421 gaggtgaggg tcccaaggcc cgtcgcccta agccaccacg ccctggtgat ggagcacgcg
     3481 ggggggacgg agctttacag ggtggtgctg gaggagccgg agaccttttt agagctgatc
     3541 ctggaggagg tggcctctgc ctggaggagg ggtttcgtcc acgccgatct cagcgcctac
     3601 aacgtgctcg tcggggagga gggagaggtc gtcatcatcg actggcccca ggc
//
LOCUS       DASM01000099           12817 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_81096, whole genome shotgun sequence.
ACCESSION   DASM01000099 DASM01000000
VERSION     DASM01000099.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12817)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12817)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12817
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_81096"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2834 a         3618 c         3918 g         2447 t
ORIGIN      
        1 gtcccgtccg aggaagacct ccaccctctc cgggtcctcg acctggaatg ggacctccac
       61 ctgggcggag gagaggacct cccggggcgg cccgatccct gcccgcctcg cgatccgggc
      121 ccccaccccc tcggcggata cgacgacctt cgcctcgatc tccctctggt cagagccgcg
      181 gccgactttg aggacgcttc gtccgccgcc cctaagaagg cccgtcacag gagaggccat
      241 catcacctcg gccccggatc tcgccgcctc cgccaggagc gcccggtcga agattctgcg
      301 gtcgacgacc gaggccttcg tcccaggagc ccggaaggtg agctgaacgc ccccggggga
      361 tacgaccttc gctccacgga cgggcctgat ggcgtggtgg ccgacggcca gctccgcctc
      421 ctccatcgcc cgcaccccga ggagtcccgc gcactggacc ggccagcccg ctcgggggtg
      481 ctcctcaaag atgacgactc tggcgccagc ctcggccgcc ttctttgccg ccatcgatcc
      541 ggcggggccc gctccgatga cggcgacgtc gtaccgctcc atctccgaga ggttcgatcg
      601 atcaggggga tagccttttc catctgcgga cgattaggac attgaagata gaggccggac
      661 ggtctggtct ggagggtaac aatttggata ttttgtggct atctgaggaa gaggttagtt
      721 cgctcttgac tatggacgag gcgatcctgg cggtggagga ggccttccga gagcacggcc
      781 tgggaacggc ccagatgccc cccaagtcct acctccacta caccaagtac cagggcgacc
      841 tcaggacaat gcccgcgtac ctggagggga tggacgccac cggggtcaag gtggtgaacg
      901 tccatccgga taaccccgcc agaggcatac ccacggtgat ggcggttgtg atcctccact
      961 ccccgaagac tggagctccc accgccgtca tgggggggac cttcctcacc aacatgagga
     1021 ccggcgcggc gggggccgtc gccgcgagat atctcgctag gaaggggtcc cggagggtgg
     1081 ggctcctcgg cgccggagcc caggctagga cccagctctt gggtattgcc aagaccttcg
     1141 atatagagaa ggtctcgatc gccgacgtat cccccgaaag gtgtagatcc ctggtagact
     1201 gggcgaagag cttccttgcg gccgagtact tcatctcctc ggatatcaga gacgtctgcg
     1261 acgccgatat cctggtcacc accaccccat cgcggagccc cctggtgaag gacgagtgga
     1321 tctcccctgg gacccacatc aacgccatcg gcgccgacgc cgccggaaaa gaggagcttg
     1381 acccggcgat cctcaagagg tcgaaggtgg tcgtcgacga catcgtccag gcctcccact
     1441 ccggggaggt gaacgtcccc ctgggggagg ggctcctcgc ccccggggag atatacgcca
     1501 ccatcggcga ggtggtggcg gggaaggtcc cgggccggat ggacgacgaa gaggtgacgg
     1561 tcttcgactc tacggggctt gccatccagg acgtggcgac ggccatgggg gtctatagca
     1621 gggccctggc gaggggcctt gggacgaggc ttccgttcct gtagccctcc cttcggataa
     1681 gcgatatata acatcccgac gaccttaagg ttgtccatgt acgcaatcat cgcctgcggc
     1741 gtcttcaagg atgagatcga ggagatagcc gacgacctcg gcttccccgc gagggtccgg
     1801 tacctggagg cggggctcca tgtcgacttc gacggcctcg ccgaggccct gaagagggag
     1861 ctggaggatt gccgaggctt cgatgggatc atcgtcgcct tcggcgagtg ccaccctagg
     1921 atcgccgaga tcctggcccc ctacaacgcc gtcctcctgc ggtgccagaa ctgcatcgac
     1981 gccctgatca ccagaaagaa ggtcgaggag atcgcaaata gggggcttta cttttaccta
     2041 tcgccggggt gggtcaaggg ctggagggat atgttcgagc ggatgagctg gagccaggag
     2101 gaggcccgct tccagctcgc ccccttcaag ggggcgatat tcctcgatac cctcggcaac
     2161 gctcaggatt acgaggaagg cctgatagaa tttctcgatt tcaccctctg cccctacgag
     2221 aagatgcccg tggacctcgc gcacttcagg gctttgatcc tggaggcaaa agagaggtgt
     2281 gaccttggac gaggagcttg aaaggatacg gagaaagaag ctcgaggaga tgaagaagat
     2341 ggcaaacgaa ccaaaggtgg actatcccaa cagccccctg aaggtgacgg acgggaccgt
     2401 cgacgaagtg atcggcagat atcccctcgt catcgtggac tgctgggcgg agtggtgcgg
     2461 accctgcagg atggtcggcc cggtgatcga cgacctggcg aaggagatgg ccggaagggt
     2521 cgtcttcggg aagcttaacg tcgatgagaa catgaagacc tccaccaagt acgggatcat
     2581 ggcgataccg acgttgctcg tattcaagga cggcaagctc atcgaccaga tggtgggggc
     2641 ctaccccaag aacaccctct caaacaaaat tcagaaatat ctctgaggct ggcagatcgc
     2701 cgcctcagaa tacggttttt ggtcttctac ctcaggccct gaccttcgag gggatgagaa
     2761 cacccgccct ctcccgagag ccgatctctc gaaggtcctt gagccggaag taggcgtcca
     2821 cctggtagag tggctttgga tctctgccga gctctgtcgg ggcctggccg acctccgacg
     2881 ggacggcgaa ggcccccttc ctccgggcgg cgatctcctg gacctccttt atcctgaagt
     2941 aggatgcgac gtcgtatagg ggcttcgcct ctctggtgag gacgttaggc tctcggcccg
     3001 tctccgacgg tacgacgaag gtccccttat cgcgggcggc gatctcccga aggtccttaa
     3061 tccggaagta ggcgtcgacc tggtagaggg gcttcgcctc tctgctgaga tcgatcggat
     3121 cgagggcgcg gtaggccacg gcgtcgaagc tcgaatccgt ggcggatctt cctgcctctg
     3181 cagcagcggg caaggccagg gcgaagatca gcagcatggc tgccatcagg gcgatatggg
     3241 aaattctcag actcatgata atcctcctgg ttgtaatagg tcggatgtgc atataaattt
     3301 ttagggtgca ccaattttta aagatcgggg ccaggcccgg cctttgcctg gcctgaatct
     3361 ttcagttcac cctgatgatc tggaggaggg atgagacggg aacccccacg atctcgtcga
     3421 cccccttctt gtcgaggagg actccgatgg cgttggtgga ggcgccgtgg tcgttcagct
     3481 cctccaccgc ctcctccagg gtcctcccgg aggtgatgac gtcatcgacg atgacgcact
     3541 cggcgtcgac gacgtcggcg aagttggagc tgaaggtccc cgagagctcc gtctcccccg
     3601 tctcctgaga ccacctctgc ttggtggggg tgtagacggc gagctccacc cccagctcct
     3661 cggcgaccat gctggcgagg gggacgccgc tgagggcgat cccgacgacg acgtcgacgt
     3721 cggagtcgga gcattccagg ctgtcgagga tcatgtcggt cagggcctgg gagacgtgcc
     3781 ggagccttac ggaactcctg ccgatggcgc tccagtcgac ggagacgtcc tttggtccct
     3841 ggacccccga cctcttctcg ccgtgggtga ggagccaggt gaccgtctcc cgggatacgt
     3901 tcagctcctc ggctatctgt ccctccacca atccctccgc cttcagctcg gcggtcttct
     3961 ctatcagact ttctatattt ttcataattt accccctcgt ctcttcttcg ccggggtgcc
     4021 acagacgggg cattcttcgc cctctaaggg cctgccgcac cccgggcacc tacaggtccg
     4081 cttcgcgatc ctccggatcc ggggcatggc gatcggctcg acctggaggc ccaggtggag
     4141 ggcggcgttc tgaagggcgt agtcgtcggt cgccagccgg gcggagagct ccagggcttt
     4201 cgccagaatc tccaggtcga cctcggagag gacggcggag tccccggtca tatcggcggc
     4261 cgccctcgcc ttctccagcg ccttagcggt ggggggctcg atcctagccc ccgatatctt
     4321 cagccgcatc cgggaccgga ggtccttcag ctccccctcg acccctggga cggtgatcac
     4381 ctctccatcg aggtccttcc cgtatatgaa gaccgacgag tctgcgacga tgaaaggtga
     4441 catcccggaa ctttttgacc ccatactact tggactttgc ctcggtgggg ggccggaggc
     4501 ggtatcgaca ggaagggacc gtccttcaga ccaaaacgct attatgatat gatgctgccc
     4561 aggggaggga ggatgccatg gcgaagaaga ggaagaagtc caagagcggt cagagaggag
     4621 cttcagacga ggcagaaggt gctgataaga cgaccaacct tcccgagaag gggaagccgg
     4681 atcggaggaa gtcccggtcc gacgggatca tcatgaccct atacccggcg gtcctggggg
     4741 tcatcatggg gttcgcgagc ctcgagctct ttgggaccgg cgtccccgag gagggggggg
     4801 tggggataac ctacccctgg tacttcgtca tgaccctggt catcgccata agctactaca
     4861 tccagaggtt cacatacccc ttcatgaaga taaacgtcga tgagttcaag gccaaggact
     4921 ggttctacgt cgagttcata gccgtagacc tatggctcgt ctcctggacc ctccttctga
     4981 actaggccgg tgggacggat gaggatcgct gtaatcaatc gggaccggtg tcagccgagg
     5041 aagtgcatcc gggagtgcga gtacttctgc ccgccggtga ggaccggcga cgagacggtc
     5101 accttccccg acggaaagcc ggtgatcacc gagaacctct gtgtcggctg cgggatctgc
     5161 gtccggaagt gccccttcgg ggcgatctcg atcataaacc tccccgacga gctggagaac
     5221 cccacccacc gttatggcga gaacggcttc gccctatacg gcctccccgt ccccgccgtc
     5281 gggaaggtga cgggcctcct cgggccgaac gggatcggca agagcaccgc cgtcgccatc
     5341 ctctcggggc tcctcgcccc gaacctgggg aggggggcct cctgggagga ggtcctccac
     5401 ctcacctccg gctcggccct cggcgactac ctccgaaggg tcgtggagaa gggggtgaag
     5461 accgcctaca agccccagta catcgacagg atacctaaga gcttcaccgg aacggtccgg
     5521 gccctcctgg agaggaccga cgagcggggc gccctctcag atctggcgga gaggatgaac
     5581 ctgttgccgc tgatggaccg ggagatctcg gggctatcgg ggggagagct ccagcgggtc
     5641 gccatcgtcg cctgcgccgc ccgggacgct gacgtctact tcttcgacga gatcagcccg
     5701 tacctggaca tataccagag gatcaacgcc gcccgggcca ttggtgatct ggcggagacg
     5761 aaggcggtga tggtggtgga gcacgacctg ggcctattgg accttctggc cgagaacgtc
     5821 cacatcgtct acgggacgcc gggggcctac ggcgtcataa cccggccgaa gggggtgagg
     5881 gtcgggatca accagtacat caaggggtac ctccccgagg agaacatccg gatccggtcc
     5941 gagccgatcg tcttcgaggt ccacgccccc cgggccgaga aggacgtccc cctcctcgca
     6001 ggttacggct ccttcaccaa gaggtactct tcgttcgtcc tggaggcgga gcggggcgag
     6061 atcaggaggg gggaggtcgt cgggatcctg gggccgaacg gtatcggaaa gaccacctat
     6121 atcaagatcc tggcgggggc ggaggtcccg acggccggcg ccctcgacct caagctcaag
     6181 gtctcctaca agccccagta cctgaaggcg gaggcggacc tggccgtcga ggggttcctt
     6241 cgaagtatat cgaaggagtt cgactcgagc cggtaccagt cggagttcgt caagcctttg
     6301 gggatcgaga agctcctgga gcagccgatg gacgagctct ccggcgggga gcttcagcgg
     6361 gtggccatcg tcggatgcct cagccgggag gcagatctct acctgctgga cgagccgtcg
     6421 gcccatctgg acgtcgagca gaggatgatg gcggcgaagg tgatgcgcag gttcgccgag
     6481 agctccgagc ggtcggtcct ggtcgtcgac cacgacatat acctcatcga cctcctctcc
     6541 gagaagctga tggtctttga gggggaggca gggaagaagg gcgtcgccca cccccccgtg
     6601 gagatgaggg cggggatgaa ccggtttttg aagggcatag ggatcacctt ccggagggac
     6661 gaggacaccc gccgcccccg ggtgaacaag cccggctcga ggctcgaccg gtcccagagg
     6721 gagtcggggg agtactacta ctacgctgcc gacgccgcct gaggcggccc gccctaatcc
     6781 attccgacct caaaatcatt ccacccattc catcccattt tatcccatat ttcgcctctt
     6841 ctatcccggc ctcaaatcgt tccgttccgt tccatcccat tccacattcc atcccattcc
     6901 accctattcc gcattccatc ccgttccatc ggaggcgacc cgctcccctc ccccccggcg
     6961 ccgccatcgc ccgggggcta tccccgattt ctaggataaa ccgccatcta catatctata
     7021 ggatctgaaa ccatcctcca ggagacttcg agatggagag ggcaaaggcc ggggacgccg
     7081 tccgcgtcca ttacgccggg aggctcgagg acgggacggt cttcgactcc tccgccggaa
     7141 aggagcccct ggagttcacc atcggcgacg ggatggtgat cccggggttt gaggcggcag
     7201 tggtggggat gagccccggc gagaggaaga gggtgaagat cccccccgag gaggcgtacg
     7261 gcccccgccg ccaggagatg gtgatggagg tggagaggag gtgcatcccg caggagtttg
     7321 agccggaggt ggggcaggtc ctccagatcg gtgggtcccg ggagaggatg ctccaggtgg
     7381 cggtctccga ggtgacggag tcctccgtct tcctggacgc aaaccacccc ctggcgggga
     7441 aggctctcct cttcgaggtc gagatggtgg agatcctggg gagcagaaga gacggcaagg
     7501 atgaggggac ggaggagacg caggagaagc aggagaagca cgagatggag gagatggagg
     7561 agatgagagg atgaggcagg caaaggaggg ggactcggtg agcctccact acaaggggag
     7621 cttcgaagac gggacggtct tcgacagctc cgagacccat ggcgcgctga agttcaccgt
     7681 cgggcggggt acggtgatcc cgggcttcga ggaggcggtg gtggggatga agctcgggga
     7741 ggcgaagaca gtcaccatcg ccccggagaa ggggtacggc ccccgaaaag aagagcttct
     7801 ggtcagaatc gcccggcagg agcttccgcc ggacctcgac ccggcagtcg gccagaggat
     7861 cgagttctcg aagggggacc agaggtttga gctcaccgtc tccgaggtca ccgaagaggc
     7921 ggtcaccttc gacgccaacc accccctggc gggaaagacc ctcgtcttcg acctcctcct
     7981 cctggagatc gagtgagccc gaggggacgg aacgggacgg gtccgaagcg gagcagcacg
     8041 gcggcgctgg aaggcggcgc gggcgaagcc ggaccgaatt cggaccgaag ccgaggcgat
     8101 aaggagcaga aggagcatgg acggcaagaa ccgaaaggac ataaagccgg ggatgaaggt
     8161 cgagatcgtc ctcaagcgcg accagaggac ggggaagctg accgaagggg tggtgaagga
     8221 gatcctgacg agctcctcct tccaccccca cgggatcaag gtccggctcg ccgacggcca
     8281 ggtggggcgg gtggcgagga tcgtggggta ggggcacata gttctttaat gaaaatcgaa
     8341 cgcatcagag ggagaagtaa tgactgatga agcttcaaag actagagatg ctatagaatc
     8401 cattttacct gatccgaagg ccagaaacca atatttgacg attttcttgg aatcgatact
     8461 cgaagcaaac aaatatggag caaataaatg gggtgcatat tataccaatg ataaaatacg
     8521 attgttggta ggtagcctga ttgtattgac cgtcgaaaaa catggtttat gggtgactct
     8581 tgataagcag ctccttgata aatctaataa agaatttgat ttgtcggaac attctgatga
     8641 ctggcgatgg gatactggta gttggtcaga atataaggcg gtaccatcta aaaatgggtt
     8701 ctatgcaccc tcagaaaaac atccgcagat ttggccgata atacggcgac tacactttga
     8761 attcattagc aatgtagcaa gaagatacgg tcaattacgc gaaagcagta aaaatacaaa
     8821 tatgccagat cttttggcgt atttgcgcta tacgttgaat caatttgttc ctgagcctgt
     8881 ttatgaggat tttaaaccat tcaacaagat aatagaacga atccaattga tccttgaacg
     8941 gaaaggccag gtgatctttt acggtccgcc tggcaccggg aagacctact gggcggaaaa
     9001 agcagcttgc gagcttgccg ccgaatcaaa tttcgatatg ggattcgacc agctctccgc
     9061 tcatcagaag tcgctcatca ccggcgatgg cgaaagcttc ggcgctgtga gaacttcctg
     9121 ttttcatccc gcctacggct acgaggactt catcgagggt tatagaccgg agcaggccga
     9181 cggccagatg atattctccc ttcgagacgg ggtcttcaag aggctctgca aagacgcttt
     9241 ggagagccct gagacgagct tctacctgat aatcgatgag ataaaccggg gagacatacc
     9301 gcgaatcttc ggcgagctga tgacgataat cgaaaaggac aagcgaggaa agtccatcac
     9361 cctccctacg agcggcgagc agttcaaagt cccaaacaac gtcctcatca tcggtaccat
     9421 gaacaccgcc gataggtcca tttctcttct ggatgcggct ttaaggcggc ggttcggctt
     9481 catcgagctg atgcccgatc cgtcagtttt gggcggggca accattgagg gcatccacct
     9541 tggaagatgg cttgaagctc taaaccagaa gatctgcgaa aacgtggcga gggacgcccg
     9601 aaacctccag atcggccact cttacctcct cagggatgat ggcacgccca tcgatgactt
     9661 ctcagagttc gccagggcat tgcacgagga tatcataccg cttctggaag aatactgcta
     9721 tgaagattac aacacccttg agaagattct agggtctgat attgttaacg tggatggtca
     9781 gaagatccga cacgagctgt tcaaacccta tccccgagac aatcttatcc agatgatagg
     9841 aagtgtatta ttttattcct ctgaaaagta aggatggatg aaaatgaacg ataagaagaa
     9901 tccggaagac gaagctcgaa agaaagaagc aaaatccttt ttaagaaagg gatattcgtt
     9961 ctataggaag aaagagaatg agaaagcgtt agaatgtttt aataaatctc tggaatttaa
    10021 cccgataaat ggatatgcat ggaatgcgaa gggttggtct ctgcatgaaa tgggtcgatc
    10081 tgaagaggct ttaaaatgcc ttgataaggc cgttgatctt aagccgaacg caccagtgat
    10141 ctggaacagc aaaggagaat tattagatag attagaaaga actgttgaag caatgagctg
    10201 ttacgaaaag gccattgaag tcagatccaa tttttcactt gcctggtcca acaaaggcca
    10261 tttattaggc cgtctgggca aacttaatga agcgatcata tgttgtgacc gagccataaa
    10321 aataaaacct aatgatgatg acgcattgaa tataaaaggg tggtgcttaa ttagccttag
    10381 caaaaatatt caatctctag attgctttga caaggctata gaaattaatc ccaaattcgc
    10441 ttctccatgg gttggtaaag gtcaagcact gcataattta aagaggtata atgaagcgat
    10501 catgtgttat gatcgagcta taaaattaga accgaaagtt gctgaaccat ggtttggtaa
    10561 aggcctagtg ctacattatc tagaaaaata taatgaagcc attgattgct acgataattc
    10621 tatcaagata tctcctgaat ctccaagtgc atgggctgga aaaggtttgt cgctttataa
    10681 acttgggagg tacgacgaag ctgtaaaatt ttatgataag gcgattgacc tagcttcgga
    10741 atacgcatgg ccttatacag ctaaaggcga ggttctttta gagactggaa gtctcaacga
    10801 atctctagaa tattgtgatc gatctattga actcaatccc aaagattact atgtttggga
    10861 gatcaaaggc aaagccttgc aggccatggg tcggcacgcc gaagccgagg aggccttcgc
    10921 caaagcccga gagctgaagg agaaagaacc cctcgacaac gaaccaaagg ccagtctttt
    10981 gcctgaaata atccaagaca ttcggcaagt gttagagcgg aaaggccagc ttatcctata
    11041 cggcccgccg gggacgggga agacctactg ggccgagagg gctgcccgag agatagcgtc
    11101 cctcgatcgg tatgggaagt ccttcgacca gctttcagag gagcagagga gcgccctgat
    11161 cgacggcggc gacgcctccg gcgccgttcg gatctgcagc ttccatcccg cctacggata
    11221 cgaggacttc ctcgagggct accggcctga agaggcggac ggcaagatga gcttcgccct
    11281 ccgggacggg atcttcaaga aactttgcaa agacgccctc tcgaatcctg gggccaagtt
    11341 ctacctgatc atcgacgaga tcaaccgggg cgacatcccc cggatattcg gcgagctgat
    11401 gaccgtcctc gaaaagaaca agcggggaaa acctataatc ctccccctct ccggcgagca
    11461 gatgcgggtc ccggagaacg tccgagtcat cgggactatg aacaccgccg atcggtcgat
    11521 cgccctcctc gacaccgctc tccggcgccg cttcggcttc atcgagatga tgccggacgt
    11581 atcggtattg ggagattccg tcgtcggcgg ggttcacctc ggcgaatggc tctccggcct
    11641 caaccggcgc atctgcgaaa gcgtcggccg ggacgctcga aaccgccaga taggccactc
    11701 gtacctgatg gaggccggaa aaccgatatc gaacctctct agcttctctc gggtggtcca
    11761 ggatgagatc atccccctcc tggaggagta ctgctatgag gaatactcga ccctggagaa
    11821 gatcctgggc ggggaggtcg tcgacgccca gagacagatg ataagatgcg agatcttcga
    11881 aggttcgatt gatggcgagt tcgtccgagc catgtcgaag atctcgaagg atggatcttc
    11941 attttgccct tcgatggcgg cgggatctga ggctacagag gacagcgtga gcgattcgga
    12001 tgacggcccg gaagaggagc gaaccgatgg ctaaagatcc gatctcagtt aatctttcag
    12061 agtgggagat ccgaggtccg gagtcgcgag gtccgcagtc ggacagatgc ctggaggggg
    12121 tttttctcgg ggatgatccc tctgtccaga aaatggccca atacctatcg gaaaaaggga
    12181 tgcttgagat atctgagctt agaaagggcc ttttgatcaa atcaaactcc tacgttggaa
    12241 gggtcgagct gggcgacctt cgcatcacaa tacgccccaa gatcgaggac gctccattcc
    12301 tccagctcct caggtacgcc tacgggcttc gagacctacg gctcttcggg cggacagatt
    12361 atctccccga gtcccggacc ttccaggacc ttctcattcg ccagctggcg gccgaggccg
    12421 aagagctgat cttgcggggg cttcaccgaa actacgtccg cctcgacgag acgcttcaaa
    12481 gccctcgggg caggatcgac ataaagagga tagcacgaaa tggcgggatc gctcaagctg
    12541 cgctgccctg cacccactac ctgaggctcg aagacagcct cataaaccag gtcctcctcg
    12601 aagggctccg cctcggcgca aggctcacgg acgatgtaat tatcggctcc aacctccgcc
    12661 gtctcgcggc gctgatgcag gatggaatct ctccggtgag gctggatcaa gagacgattg
    12721 gaaggctccg ccgccagatg acgaggcaga cggtggccta ccggccgtcg atatcgatca
    12781 tcgagatcct cgtcgccgcc cagggcctct ccctgga
//
LOCUS       DASM01000100            8557 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_75916, whole genome shotgun sequence.
ACCESSION   DASM01000100 DASM01000000
VERSION     DASM01000100.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8557)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8557)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8557
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_75916"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1661 a         2658 c         2892 g         1346 t
ORIGIN      
        1 gccaccatcc tccgggagat ggatatcgag cacccggtgg cgaagatgat cgtcgaggtg
       61 gcccgggccc aggaggatga ggtgggggat gggaccacca ccgccgtcgt cctcgccgga
      121 gggctcctgg agaacgccga ggagctgatc gacatcggcg tccatccgac ggtgatcgtc
      181 caggggtaca aggcggccca gaagaaggcc tacgagttcc tggaggggat ggcggtggag
      241 gtctccaagg gggaccggga gatcctgaag aggatcgccg agaccgccat gaccggaaag
      301 gggatcgagg tcttcaagaa ggacctcgcc gagatctgcg tcgtcgccgc cgcggcgatc
      361 gaggatgagg ggaagttcga cgtcgaggag agggtcagct tcgtcaagat cggcggcggc
      421 tcggtgaagg atacgatcct cgaggagggg gtcgtcctcg ataaggagag gctcaacccc
      481 gagatgccca agagggtgga aggggcgaag atcgccctcc tcgacagcac cctggagctg
      541 aaaaagctct ccaccgacgc caaggtgacc atctccagcc ccgagagcct cagcagcttc
      601 cgggaggggg aggacgaggt cctgagggag aaggtcgagg ccatggcgaa ggtcggggcg
      661 aacgtcgtct tctgcaagca ggggatagga gcttacgcct cccgatacct cgcaaaccac
      721 agaatcatcg ccgccaggag ggtcagcgac gaagagatga agtttctggc cctggccacc
      781 ggcggaagga tcgtcaccga ccccctggag atgagggcag aggacctggg gtccgcagac
      841 ctcgtcgccg agaggaaggt cggaagggat aagaagatga tcttcgtcga ggggtgccac
      901 ggggcgaagg cggtgacggt ggtcatccac ggatcgagcg agcagctccg ggagaacatc
      961 gagagggccc tggaggacgc cctctggtcg gtggcgacgg tcctggagtc gaagaagatc
     1021 gtccccggcg gtggggctcc cgagatcgag gtggcggaga ggctccggca gtacgcctcc
     1081 accatcaccg gcagagacca gctcgccgtg aaggccttcg ccgacgccgt cgaggagatc
     1141 cccttcaacc tggcggagaa cgctggcttc gacccgatcg acgtcctcgt ccgcctccgg
     1201 tcgagccacg gggcggggga gaggagctac ggcctggata tcaacaccgg aaagcccgcc
     1261 gacatgatgg aggcgggggt cgttgagccc ctgaaggtga agacccaggc gatcaagtcc
     1321 gggaccgccg ccgccacttt agtcctcagg gtcgacgacg tcatcgccgc caaggtcgag
     1381 gggctgaagc cgaagccagg ccagagcccc cacgactaca ccagaaaccc gatgatgccc
     1441 cccatgaggt agggagggga acggggcgcg gggtccggag gggacctctt gcccctccgg
     1501 ccttctgata ggtggggaac ttttcgtcga cgacatcttt catctcgact ggcaagggtg
     1561 gtttatgaag gtgaagaaga gaggaaaaaa agaggaggag cccaccgagg aggtggggac
     1621 ggccccttcc cggaaggtcg gcgatgaggc ggagatcgag ggggatgatc tggagctggc
     1681 gaggcagctt gctgaggcgg agaggctggt cgaggagctg actgatcagc tcctccggtc
     1741 cagggcggag ctggataaca ccgtcaagag ggctgcccgg gagagggagg cgtcgaccct
     1801 attcgcctca gagaggctga tgacgaagct cttgcccgtc ctcgacagcc tcgatcaggc
     1861 cgcaaagcac gtcgaaggga tggataaggt cagaaagcag cttctggacg tcctccggac
     1921 ggaaggcctc tcgccgatcg aggcgaaggg ggagaagttc gacccctaca agcacgaggc
     1981 tctgatgatg gtcgagtccg acgaggatga agaaggcacg gtcaccgagg aggtccagcg
     2041 gggctacgcc ctcaattcga aggtgatccg cttctcgaag gtcctcgtct ccaaaaaacg
     2101 gtaaatattc ggcagttcta ctccaacaag gtgataacgt gtccaagatc ataggcatag
     2161 acctgggaac gagcaactca gccgccgccg tcctcatcgg cggaaagccc accatgatcc
     2221 ccagcgccga gggtacgagc atcgtcgcgg gggggaaggc cttcccctct tacgtcgcct
     2281 tcaccaaaga cggccagagg ctggtgggcg agccggcccg gaggcaggcg gcgacgaacc
     2341 ccgaagggac gataaccggc atcaagagga agatgggcca ggactatcgg gtggaggtct
     2401 acggcaagtc ctacgctccc caagacatct cctccttcat cctccggaag ataaaggatg
     2461 actccgaggc cttcctggga gagaaggtgg agaaggcggt gatcaccgtc cccgcctact
     2521 tcaacgacaa ccagaggcag gcgaccaaag acgccggcac catcgccggc ctcgacgtcg
     2581 tcaggatcat caacgagccg acggccgcag ccctggccta cggcatggac aaggagggag
     2641 aggcgaccct catggtcttc gacctcggcg gagggaccct cgacgtcacc atcatggagg
     2701 cgggggaggg ggtcttcgag gtcaagtcga cgagcggcga cacccagctc ggcggccggg
     2761 acatggacga gatcctcacc aattacgtcg tcgagaggta caggcaagag tcggggatcg
     2821 acctcaaggg cgaccggatg gcgatgcaga ggctgaggga ggcggtggag atcgcgaaga
     2881 tcgagctatc atcggtcctc cagacgacga tcaacctccc ctacatcacc gcggaccaga
     2941 ccggtcccaa gcacctggag atgacgataa ccagggcgaa gctggaggag ctgataaagg
     3001 aggtcctgga gcggtgccgc aaccccatga tccaggcgat ccgggacgcc aagctcgata
     3061 agtccgggat cgacaagatc gtcctcgtcg ggggccccac caggatgcct gccgtcagga
     3121 agttcgtcac cgagttcatg ggcaaggagc cggagggggg cgtcgacccg atggagtgcg
     3181 tcgccatagg ggcggcggtc cagggcgggg tcctgggagg cgaggtgaag gacatcctcc
     3241 tcctggacgt cacccccctc tccctgggga tcgagaccct cgggggcgtc gcgacgaagc
     3301 tgatcgagag gaacaccacc atcccgacga ggaagagcca gatcttcacg acggccgccg
     3361 acaaccagac gaccgtcgag atccacatcc tccaagggga gaggcccctc gccaaggaca
     3421 acacctccct ggggcggttc accctctcgg ggatacctcc ggcgccgagg gggatacccc
     3481 agatcgaggt ggccttcgac atcgacgcga acgggatctt gaacgtctcg gccaaagacc
     3541 tcgccaccgg aaaagagcag gcgatcacca tctccgcccc ccacaagctc tcccagaacg
     3601 agatcgagcg gaagatccga gatgcggagc agcacgccta cgaggatacc aggaggaagg
     3661 aggagatcga ggcgaagaac caggccgaga ccctcgtcta caccgcggag aagaccctca
     3721 aggatgcggg ggaggtggtc accgccgacc agaaggagaa gatcgagagg gcgatcgggg
     3781 aggcgaagga ggctctggcc ggcggggata tcgccaagat aaaggagaag acggaggccc
     3841 tccagaacgc catctacgag ctctcgacgg cgatgtacca gcgggcggca gagcagcagc
     3901 agcgagagca ggagaaacag cagcagagcc agggaggccc caccgcctca ggggagacga
     3961 tagacgccga cttcgaggtc gtcaacgaca agagatgagg tccgcgtccc gggtagaagg
     4021 aggacggccc cttgggagcc gccctccctg gccggaggat gagaggcaaa tgacgggtgg
     4081 gattttagga tctggagagg ataggcggtg gcggggagca agagagacta ctacgaggtc
     4141 ctcggggtag gaaaagagac ggaccagaag gagatcaaga gcgcttaccg gaagctagcc
     4201 ctcaagtacc atcccgatcg gtcccaggag cccgacgccg aggagaggtt caaggagatc
     4261 tccgaggcct acgccgtcct ctctgacccc gacaagagga ggcagtacga tcagttcggc
     4321 cacgccggta tcgacggccg ctactcccag gaggacctct tccggggggt ggacttcgag
     4381 gacctcctcc ggggcttcgg cttcggcggg gggtttggcg gcggcgggtc catcttcgag
     4441 atgttcttcg gaggcggaag ggggggccgc cgggggccgt cccgggggcg cgacctccgc
     4501 tacgacctgg agatgaccct ggaggaggtg gcgacgggcc ttgagacctc catcgaggtt
     4561 ccccggatgg aggcctgctc tacttgcggc ggcaccgggg cgaagccggg aaccagtccc
     4621 cagacctgtg accagtgcca gggaacgggc caggtgacga gggtccagaa gacccccttc
     4681 gggcagatga tgacctcttc cacctgcccg aagtgcgggg gccgggggca ggtcgtctcc
     4741 gacccttgct ttgagtgccg ggggtcgggg cgggtccgga agacgaggag gatcaacgtc
     4801 aaggtcccgg cgggggtgga ctccggccag cacctgaggc ttggcggcca gggcgaggcg
     4861 agccccgacc ccggcggcga atccggcgat ttatacgtct tcgtcaacgt ccgcccccac
     4921 cccctcttca agagggccga cgacgacctc ctctacgagg cggacctctc catcacccag
     4981 gcggctctgg gagccgacct ggaggtgccg accctcgacg ggatgcgggc gaggataaag
     5041 gtaccccccg ggacccagag cggctcggtc ttccggctga aggggaaggg gatcccccgc
     5101 ctccacggct tcggcgggac cggcgacatg cacgtccggg tgaacgtcaa gacccccgcc
     5161 accctcaccg ggaggcagag ggagctgctg gaggagctgg cggtcgagtt cggcgaggga
     5221 gagagggagt ccaaagggac cgagggcgcc agggggaagg agaagcctgg agagaagaag
     5281 gagaaggggc tcctcggcaa gatcgtagac gaggtgaagg gggcggtcca gtgacccgtc
     5341 cccgccgata aattggatca tcatccctga tctccaaccc ttaatcggcc tccttcggcc
     5401 cctcatccct cgccttctcc aggatctatc tctcccgcct caccttctga tcgcctccat
     5461 cttcgtctcg atggagatct ccttgtccag ccggtggtcg atcttgatgg tggtgacgat
     5521 cctccccgcc cccatccccg ccaggacggc gtcggccttc tccaccacct caaagatctc
     5581 tttcatcgaa cccgcctcga tagaggttga catggggccg gggacgaact tgagccccga
     5641 ctccttcagg acctggtgga ctgcccggac gtaccggctg gcgctcgtcc ccgtccccat
     5701 cgttatcatg gaaagctccg cgactatcat ggactggatc tcctaccgcg gacgataaaa
     5761 aagtttttac gaatcaggcg tcagcggatc tccctcagga tggcgtccgc cgaggccgcc
     5821 gggttcggcg actcggtgat actccttccc acgatgaggc tgtcggcccc ggctttcagg
     5881 gccgccgaag gcgatccgcc ctggaccccc acccccggcg agatgatcgc ccggtccccc
     5941 acgatcgacc tgacgagcct tatcctctcg ggcctcgtgg cgggggcgac gaccccgtcg
     6001 acgccgcagt ccgctgccat ccgggccagc ctctcggcgg cgggtcccat cagctcctcc
     6061 gccccggggt ggctcatctc ggtcacggcg aagacgaggc ccccgcattc gcgggcggcg
     6121 tcgacgcagg cggagaggct gtcccgcccc acaaaggcgt gggcgatgat cccggcggcc
     6181 cccgaatcca ggacctgctc gcagatgagg gtgttggtgt tggggatgtc ggcgatcttg
     6241 aggtcggcga tgaccggcgc gatcctggcg atctcgccga cgatgtcgag ccccgccgcc
     6301 aggatgaggg ggtagccgac cttgacgtag tcgacgcgat ccttcagggc agtggcgagg
     6361 tggagggccc tatctctcga caggacgtcg agggcgagga tgaggcggga gtttcggatc
     6421 atgggggtga ggtgggatgg acgggctaat tatcttttcg acctttaggc tcatgtgacg
     6481 gaagcgatcg gcccggcacg tccggctcat tcggctcatc ccttctttcc ggtcgccttg
     6541 aagaaggtgt agcagaagac cccgtccttc tccgccgtcc ggtacaggtc ggatactccc
     6601 gaatcgaact cctcctcccc catcaggccc gccgccaggg ccgagtcccg gaccccctcg
     6661 atcatggcgg tgaaggtcct ccgagtgaag ccgtcggcca tggcgggcct ggacccgtcg
     6721 gcgtagacca tccgggggga gacggagacc tcggcgaacc cggcccggac gaggagcgga
     6781 taaagctccc ggccgatcat ggcgttcccc cccgcccgct cctggagggt cacctggcag
     6841 ccgatggccc tccgggcggc ggggctgtcg gggtaaaagt aggcggagcc gtggtccccc
     6901 tcgatcgccg tgatcgtccc gccgggtttg atgtaatcct tgaggatgga gagggcttcg
     6961 acgggccggg ggaggtgctc caggacgaag cagacgaata aatggtcgaa ggaggctttg
     7021 cggaatggga gatggaagac gtccccctgg aggacccgga cgtttccgat ccctgcggcc
     7081 gccaccctct ccctcgcctc ggaggcggag gggcgggaga tctctagggc tatgatcctc
     7141 gccccggggc tgtttctcgc gagggggagg gtctgggccc cgacgccgca gcccgcctcc
     7201 aggacgaggg agccgggcgg gtaagtggtg tcggagtgga ggaggtcgac cagggtcgcc
     7261 gcctggtcct ggaggcgggt gctctcgcgg gggtggtacc cgtggacgta ggggtcggcc
     7321 atggacggta atctcttggc tgcttatatc aggatttccg cggagggtga gcccaatggc
     7381 agagtggtcg caggacatat tccttcaatg aaaactccat ctaaattccc atttcaatac
     7441 accctcctcc ctggcctcct ctcccactcc tccagaacct cccggcaccc ctcccggtcc
     7501 acatttacca gcaccagctg ctcagcctcc gcctcgcccc ttatgacctg gtagatgagg
     7561 ctgtcgtcga agccgatccc ggccacgtcc tcccgggtcg tcccgtagac gagccttttg
     7621 atcctcgccc agaatatggc ggcaaagcac atggggcagg gctcggaggt ggagtagatc
     7681 tcgcaagtcg agaggtcgta gcgccccaaa atcgaggagg cctcccggat cgccaggatc
     7741 tcggcgtggg cggtggggtc gttccgcctc aggacctcgt tgtgggcctg cgagatgacg
     7801 acgccgtttc ggacgattac ggcgccgaag gggccgccgt cgtccttttc gatcccggcc
     7861 cgggcctcct ccagggcgat cttcaggaac tcgttcatcg acggcgcctc ttcattcctt
     7921 cctcccttcc tcccttcttc atcatttact ctattcatcc cttcattcat ccgcccggac
     7981 gtttgatcct actttttcat ccggctatat catccggcta tcaggtccat gacgtccctt
     8041 cgggggctgt ggccgaccgc cttctccgcc tcccccatcc tgacgagcat ctgtatcgtc
     8101 tggccctccc cggagaacct ctcgtggacc tcctggtaga gcgcctccat ctcggggtac
     8161 tcctccagga cctggctcac cggctgcatg ctgatcccca tgatggtccc ggcgagctgt
     8221 agccgctggt agacgatccc cgccctcacc tggtcggtcc tgctgttgcc gtcggtgacg
     8281 acgatggcgt aggcgggggt ctgatgcagc cgctccacct cccccgtcct ccaggagtct
     8341 gccatcctct cgtcccccag ggggattatg gtgccaagcc cctggacgag ggcctggagg
     8401 aggcggggca tcccctggga gtcgagggtg aggccgtccc ggtggaggtt cttctccccc
     8461 tcgttgatcc tgaagatctc cccgctctcc ctcatgggac cgggaagccc cgactccacc
     8521 tcgacccccc ggaccgccag gtcccggatc gcctcaa
//
LOCUS       DASM01000101            8577 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_52657, whole genome shotgun sequence.
ACCESSION   DASM01000101 DASM01000000
VERSION     DASM01000101.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_52657"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    5438..5479
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1773 a         2681 c         2474 g         1607 t
ORIGIN      
        1 ggggcccggt ggaggccgaa gccgtcgggc ttcgagtaca gagaggcggt ctggtgccag
       61 ttctctccgt acctcaggtc ctggaccttc tcgtacgtct ccactaggta gcgggggccc
      121 tcaccctgga gggccgaaag cttctccgca tccctggtta ttaaaaagta aagggcttcg
      181 gtggcgagga gccgtctcgt ctcctcgacg aggcgcccct cggccatcag atcctccatg
      241 acgatccccc accagcccga acggatgagg accgcagagc cgcaatttgc ggcctcccgg
      301 acgatcactg atgaggacgg cgacgaatcg aagttcagat cgaagaggat gaggctctcc
      361 ggcccccata cccccccacc ctccggggag gcgagatccc tcaggacctc ggaggcggcg
      421 gtcctcttga tctcaatccc tctatcctcc aggtcccgga ggagatcctt cacctcgggg
      481 ggctcatctg ccaggacgac aacttgccta atctgcatga aaaccttggt cttaatggtc
      541 tccaggttat tttaacattg cccctcattt gcgattttga ggctagaggc ggcgaatttc
      601 atccaacccg tcgggcccga ggcggtctat ttggcaaccg gacttgacga attggcggcg
      661 gccggatcga cggggcaaat atttaagtcg tcccggcggc gctcttctga ccatgatctc
      721 ccccgaagac gcccgggagg cgatagaacc ccaccctcaa ggggtcctca tccggtttga
      781 ggtggctccg gggtcgtcga ggttcctcgt cccctcgggc tataacccct ggcggaaggc
      841 cttggaggcg aagctcaccg agagggccga gagggggagg gcgaaccatc agctcgaatc
      901 cgcagtagcc gagatcttcg ggatccccag ccaccgggtc caggtcgcct ccggcaccaa
      961 gagctcccgg aaggccgtca tcgtcctggg ggtcggcccg gaggaggcgg cgaggcggct
     1021 ggcggccggg gggctcccgg ccccatgacc cgaagaaggc gagccctccg gaggagcggc
     1081 gacgagggga ggggcaggcg ggccgggatc gaccccgacg ccctggagga gcttctggcg
     1141 gggatcctcg aggcctcggg ggagggcgca gcgatcgtcg tcgaggggga gcgggacgag
     1201 cgttccctcc gggagctggg ggtggagggg ccgatcgtca gggcctcccg gcggccggcg
     1261 ctggaggtgg cggaggacgg ggtcatgggc tacgaagagg tggtcatcct caccgactgg
     1321 gaccgggccg gggacgagct ggcgaggagg atcgagctcc acctccgagg gatgggggca
     1381 agggtcgata ccgagacgag ggagcggctg aagcggatgg tccgccggga gataaaggac
     1441 gtcgagagcc tcagcgggtt cgtcgaaagg gtgaggtctg agatatgtcc cgaagagctg
     1501 ccgtgaccgg cgcccccggc gtcgggaagt cgaccctggt ggagagggag gcggccctga
     1561 tcggcgggag agcgggggac atctctgcca gggaggtccg gatcggtggt agaaggatcg
     1621 actttcagct ctccgacctc ctcaccggag aggtagggac cctcgccacc ctcgaggggc
     1681 ctggaccgaa ggtcggcagg taccgggtcc acctcgccga cctggagggg tgggggcgag
     1741 ggcgatcgag agggcggtcc gggaggcgga gctggtggcc atcgacgagg tggggccgat
     1801 ggagctcctc tccgaggagt tcgtcggagc ggtggaagcg gccctcgact ccgataagcc
     1861 gatcctagcc gtcgtccaga tgaggtcgga gcaccctctc gcaaagaaga tccgggaggg
     1921 gttccggctc ttcgtcgtaa ccccggatag ccgggaccgc ctcgccgggg agggtgccgc
     1981 cgagacgagg ccctgaggct tccgcctcag gagccgccgt ggatctcctc gagctttgtg
     2041 atattccgga tctcctccct caggtccggc ggaaggccct tgatctccac gtcgaggaag
     2101 ccccggacga tgacgctcgt cgcctcgtcg ggggatagcc cccgggccat caggtactcc
     2161 aattcctgct gggagatctt cccgacggcg gcctcgtggg acatgtcgag gcccccacac
     2221 cagccgtcca gctccggtat ggagtagatc cttcccttct ccgtgaggat gaggccggtg
     2281 cactcgacgt gggcccggat atcggggact ttgcccacca tccggccccg gttgatgatc
     2341 tcgccgccgg tggatacgac cctggatatc gactccgcgc tggtcttggg cgcctccaat
     2401 atcgtctggt ttccgatgtc gatatttccc cggcgggcgt acatgatggt gctgaagctg
     2461 gcgacggccc cctccccggc aagggtcgcc gacgggaagg cctggaggct cctgacgggg
     2521 gtgagggtga tgtagttctc ggtgatggaa ccgccggcct ccaccaggct cgcccccctc
     2581 ggcctcacct ccacctcctc cccccagttg tggaccatgg tgaaggtgac gtgggcccct
     2641 ctcttgacgt agacctcgga gacccccatg tggagccccc tcccggcgtg ggagggggtg
     2701 gtgcagccgg tgatgatgtg aagctcggac ccctcctcgg cgatgacgac gttgtggacc
     2761 cgctgccgct tgttatgacc ccccataaat aggcaggtct tgaggggaaa tacggtcttc
     2821 gtcccgggct tggcgcggat gaagtagccg gcgggcgggt gcatccccac gtcggcggtg
     2881 tacttgtcgg cgtcgatggg gacgagcttc cagaagtagt cgacgaggta gctgtgctct
     2941 ttgagggcag aggcgatgtc catcacctcg accccctcca ccgcggagcc cctgtggacg
     3001 ggggactggt cgacctggaa gtagtggccc gaagcctcgc cggcgaggct caccccggcg
     3061 ctgagggcag cggtctggac cgcctccgga agcatctcca gctctgtgat ctctccgacc
     3121 tcctcggcct ccgcctcgta ggtatcgaga tctatatccg tccccagggc ggccttcttg
     3181 tcggcggcct tcttcgcctt ctcctctaga cgggacatag acatgctgcg cacccctcgt
     3241 atccgttggc cttgatctgt tccaaaatct ccttcgggct tcccgagcag acgatcgtcc
     3301 cctcgatcat gacgtgggcc ttgtccgtcg ccatgtagtc gaggatgtgg ccgaagtggg
     3361 tgatgatcac cccggacttc gtcctcaagg agggcttcgc gtccttctgg gtgaggatgt
     3421 tgatcgcccg gccgatcacc ttgatgttct ccaggtcgac cccggagtcg ggctcgtcaa
     3481 gcatcacgaa gtccggctcc tgggcgagga gctggaccat ctccgacctc ttcacctcgc
     3541 cgccggagaa gccccggttc acctcccggt cggcaaagtc ggtgaagtcc aggtcctcga
     3601 ggatcttgtc ggcgtctgcc tgggggttac agagcttgat catgtccaag accttcagcc
     3661 cccggatggt gggcgggtgc tggaaggcga tcccgatccc cagccgcgcc cgctcgtgca
     3721 tcgacaggtg ggttatgtcg acgcccttga aggtgatcgt ccccctctcc accacgtacc
     3781 gggggatccc cgctatggtg ctgaggaggg tcgtcttgcc gcagccgttg gggccgaaga
     3841 gggcgtgggt ctcacccttc cccacgctca tgtccacccc tttcaggacc tgcttctccc
     3901 ctatcctcac ccagagatca tcaatttcta acgttgtacc gcctcctgtc gccgaatcgt
     3961 cataagctct acattttcgc ctgacatgat ggatctcgtc actaataacg tcttcccttt
     4021 tcgcgggcga aggttgagac ttgcaagttg gattacacgg caaaaatggt cacatgctcg
     4081 cgaaatcgca gattggcctg atccccggcc gggcggcgat cgtccggctg cggcccgtcc
     4141 tcgatccgcg ccggtagcgc ctgtgggttc gagggcgaga atgagccgtc agcgtctcat
     4201 cttatcgagc gaccccgggc aactatcgcg cattggagag gattgcggcg aggaattcga
     4261 gcgagagaga tagatgcaaa gtaaaataaa aacgtttata aatgatgttt ccgaatatat
     4321 aaaattttta taagcagcat ctacataaga aacgatcgac aaaaactcat ctggtgatga
     4381 ggaggctcta ggtcgaatct gccgcaagat ggatgacttt accttttctt tggatgaacc
     4441 ggatatcccg cctcgaaata agcggtggta taggctaatt acttaagagg atagcgattg
     4501 cagttctgat atctgagaga ataaattcta tacagtctcc gagaaaaccg ccatcctgta
     4561 gtcggcacct ctttcagaag gtcgctcaaa attttctcca tcaacgatag tgatttaaac
     4621 accgatcaat ctaggtaaaa ttgcgtgatg ttggatctgc cggatccgtc gctggagcaa
     4681 gattatggga gatcgtaatc attttcttgg aacctcgccg ggaggtctct taattctgat
     4741 tctgctcgtg ggctctgccg gggccgcgac ctggaccgtc aaccctggag ataacatcca
     4801 ggtctacatc gatgtcgcca gccccggcga catcatccgg gtcaatagcg ggacctatac
     4861 tgaaaacgtg aatgtagaca agcagctcac tctggagggc gtggacaccg gcggcggcct
     4921 gccggtggtg gatgccaact tcggcgacgc catcaccctg atcgcagatc ggtgtaccgt
     4981 taaggggttt gtggccacga aatgctctac ggggatttgg gcatattcta gctacaacac
     5041 catctcgggc aacaccgcca ccggtcccgg caacggcatc gtgctccttt cctccagtaa
     5101 caacaacatc tcgggcaaca tcgtcaacga cagcaacacc ggcatcttgc tcctttcctc
     5161 cagcaacaac aacatctcgg gcaacatagc cacgaggacc atcgccgacg gcatcgtcct
     5221 cagttcatcc agcaacggca acatcatctc gggcaacaac gtcgccgaca gcggcagcgg
     5281 catcggcatc tattcctcca gcaacggcaa catcatctcg ggcaacaccg tcaccggcaa
     5341 cgacctcgac atctacctcg attactccag caacaacata atctcgggca acaacgtctc
     5401 cgacagcgtt cgcggcatat acctctctca atacagcnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnna aacacatacg gcatatacct ctctgaatcc agcaacaaca
     5521 ataatatctc gggcaacacc gtctcggaca acacagacgg cgtcagcctc gaatacgaca
     5581 gcaacggcaa cacgatctcc gacaacatag ttactggcaa ttcccgatac ggcatttatc
     5641 tcggttattc cagcagcaac gtcgtctcgg ccaataccgt caacggcaac gaccgtggca
     5701 tctacctcaa ttttttcagc aatgacaata tcatccacct caacatattc agcaacagtc
     5761 tcaatgcccg ttcgaccggg accaaccgct ggaacgccac caccaatcag acctacgagc
     5821 acaacggccg gatcctcacc ggcctgcttg gcaacatctg gtccgactac gacggctttg
     5881 attgcgatgg cgacgggatc ggcgatactc cttatgccaa gatagagcaa gggccccacg
     5941 aggtagacta tcatcccatc ggcggcaggg ctgctggccc tgccatcgag gtcgagaagg
     6001 tcgccaacag gacggaggcc ggagtcggcg aggtgatcga ctacaccata tgggtgaaca
     6061 acaccggcaa tgtcaccctc acaggagttc gtgcctggga caacctgacc ggggctgtct
     6121 gggatctggg aaccctcgaa cccggccaga accgcaccaa caccaccagc tatctggtca
     6181 aagagaccga cctgccgggg ccggtgacca acgagctttt ggcgaacggc accgacccct
     6241 gtcgcaccga ggtgaacgac tcggcgatcg agacggtggc gatcctttac aacgcctcga
     6301 tgaacatcac caagacggcg aacacctcgg gaccggtctc attgggtgat attatctggt
     6361 atacgatcaa cgtcaccaat acaggagatg tgaacctgac cggggtcaaa gttgtcgacg
     6421 atctcttggg ctcgggctgg ccgatggtca ctcttgtgcc tggcgagagt aacattttcg
     6481 ggtccgacta ccagctcaag gaagcagacg tcaccagggg cgttatcgtc aacaacgcca
     6541 cggccaacgg caccgatcct tgcggcaagg ctgtagggcc ggtgaaggcc aacgttacaa
     6601 tcgacgtcaa caaattctgc atctcgggca aaaagatcat caactgcttc caggccgacc
     6661 tctcaggctg gaatatcacc ctctacgatg ccagtggaac cagcctcttg aactggaccc
     6721 tgaccaacgc cagtggatac tatcagttct gcggccttga tcctggcgac tatacagtct
     6781 gcgaggaggc caggcccgac tacaccaacg tcacccccac ctgcatcagc gtcagtctgc
     6841 acgataacgc gcccgacatc gacttcgaga acgatcccct cctctgcatc tcgggcaaaa
     6901 agattatcaa ctgcttccag gccgacctct caggctggaa tatcaccctc tacgatgcca
     6961 gtggaaccag cctcttgaac tggaccctga ccaacgccag cggatactat cagttctgcg
     7021 gccttgcccc tggccaatac atagtccgcg agacgctcaa ggacggctat accaatgtca
     7081 cccccctcag tggtatccca gtgggtctca tatgcgataa catgaccgac atcgacttcg
     7141 agaatgatcc cctcctctgc atctcgggct acaagatcat cgacaactgc cctggtgcca
     7201 acgtatcagg ctggaatata accctgtacg acgaaagcgg aaccaacctc ttgaactgga
     7261 ccctgaccaa agataccggt tactacaagt tctgcggcct tgcccccggc caatacatag
     7321 tccgcgagac gctcaaggac ggctacacca acgtcacccc cctcagtggc atcccagtgg
     7381 gtctcctctg cgataacagg acctacatcg acttccacaa cctgcccctc ctctgcatct
     7441 ggggccacaa gatcatcaac cacgccggag ccgacctctc agactggaat attaccctgt
     7501 acaatgccaa tgaccagctg ctgaactcga ctctgaccaa cgagaccggg tactaccagt
     7561 tctgcggcct tctgcccgcc agctacaaag tctgcgagac ggcccggctc ggctacatca
     7621 acgtcacccc cccttgcgaa aacgtgaccc tcctctgcga taatgcgatc gtcgacttcg
     7681 agaacaagcc ccacctgacc tgcatcggcg gctacaagct cgaccccttc ggcaaggggc
     7741 tcgagggctg gacgatcttc gtcgattaca acgacagcgg gaccctggac ccgggcgaac
     7801 cgtacaacgt aaccgacgcg gacgggcgct ggcagatctg cggcctggtg gtgggcagcg
     7861 ttcccaactt aaccgaggtc ctccagcccg gctggaagcc ggtgaacccg gccggcggct
     7921 ggcagctggt agtggtccgg tccaacgata cctacaaaaa cttcaccaac gagcgttacg
     7981 acctctccat ctccggctac aagatcaaca actgcacccg agagacgatc tcgggctggc
     8041 cggtccagct gataaacgag gccggagccg tcatcaacga gacgacgacc gacgccgacg
     8101 ggtggtacgc cttcaccggt cttgtccaag gcaactacac cgtctgcgaa gccctcctgc
     8161 cgggttggac taacgtcacc cctagataca tcgaagttcc tctcggagcg accaacgtct
     8221 cagaccagaa cttcaccaac gatccgccgg gctgcatatc cggccacaag ttcgactcga
     8281 caaacaataa aggcgttgct ggctggacca tggttctctt caacgccaca ggagcgaagc
     8341 tcgacgagaa ggagaccgac gccacaggcc gctacgagtt ctgcggcctc gttcccggcg
     8401 actataccgt ctgcgaagct tctcgggata actggacgaa cgtgacgccc ctctgccaga
     8461 acgtcaccct ccttccctgt ggtggagcga ccctggactt ctacaacgat ccgaggaacc
     8521 tcaccatcac gaaagtggcg gacaaactct ccgtccagga gtgcgacgag gtcacct
//
LOCUS       DASM01000102            4540 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_23243, whole genome shotgun sequence.
ACCESSION   DASM01000102 DASM01000000
VERSION     DASM01000102.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_23243"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          751 a         1497 c         1341 g          949 t
ORIGIN      
        1 ccatcagccc catctcgatg gggaagccgt cgtcgtcgta ggtgtctgcg gttattatct
       61 tcaccacgct catcctccga aactcctggg gagagatgag cccgaaactt atcttgccta
      121 ttctcttcgg gacggtcatg ttcacaccta aactgcgtcc tccagaacca gccgaggggc
      181 gacgccgaga gatttgatct cgtccagcag gagcttgaag gcgtagctca tctccacggg
      241 gtatatctcg gtgtccgagc cgcagaccga gcagtagtcg acgttcctcc ttttatcgtg
      301 gatggcgatc atcccacagc ggctgcaaac gagctccgtc accttgtcgg actcgtcgac
      361 gagccgctcc ttgagggcga gggccgcccc gtgggcgatg aggacgtcgc gctccatctc
      421 tccgaaccgg aggcccccct cccgggcccg accctccgtc ggctgccggg tgaggacctg
      481 gacgggtccc ctcgaccttg cgtgcatctt tccggcgacc atgtggtaga gcttctggta
      541 gaggatcacc ccgacgaaga cgtcggccat gatctgctcc ccgttgatcc cgttgtacat
      601 cacctccctc ccggtgtggg agaagccgaa cttggtgagg gcggccctca tgtcggtctc
      661 ctcctccccc aggaaggcgg tggcgtcgac gaacctcccc tcgagggatc ccatcttgcc
      721 cccgatcatc tccaggacgt ggccgacggt catccgcgag gggatggcgt gggggttgac
      781 gatcaggtcg gggacctggc cagcctcggt gaagggcatg tccgccgggg ggacgatcag
      841 cccgatcacc cccttctggc cgtggcgcga cgcgaacttg tcccccagct ccgggaccct
      901 ctggtccctc gtcttaacct tggcgagccg gtttccgttc gaggactcgg tgaggatcac
      961 cgtgtccacc acccccttct cgttcgacct catcgtcacc gaggtctcgc gcctctgctg
     1021 aggggtgatc ccaaactccg tcggctcctc caggaaccga ggcggcgagg tcttccctat
     1081 caggacgtcg ttgggggcga cgacggcgtt ggggttgacg aggccgtcgt catccagctg
     1141 cgagtaggcc tccgtccccc gggcgccccg gacctccatg tcggggatct cgaacttgtc
     1201 ctcctggccc ccggggtact tccgctcctc cgcctccgat acccggaaga agtggctccg
     1261 gcctaggccc cgctcgatgg agccgcggtt gaggatgagg gcgtcctcga tgttgtaccc
     1321 ctcgtagacg aggacggcga cgacgaagtt ctggcccgcg ggcctctcgt cgaagccgac
     1381 ggcgtcggcg gtcctggacc tgcagagggc cctctggggg tagtggaggt agtggccccg
     1441 ggtatccggt cgtagcttag tattggccag cggcgtcccc aggcactgct tgatcatgcc
     1501 cgcgcccatg gtgtttcggg ggctggcgtt gtgctcgggg taggggacga ggccggtgca
     1561 gatccccagg acgagggagg ggtccacctc caggtgggtg tggtcggggg ttatctccat
     1621 ctcctcgatc gccaccaggg cgttctcctc ctcctcggcg tcgaggtact cgacgagccc
     1681 ctctcttacg agatcgtcga aggtgatctc gccccgccgg agcttctcta cgtgctcctc
     1741 ggtgacgagg acggagccgc cctccaccac gatgagcggc ctccgggctc ggccggagtc
     1801 ggtgttgatc aagatctcgt tcgtcctcgc gaagtaagat acgttgatct ggttgctgat
     1861 cttgccgccc cgccggagcc gcctcatatc cttggcgagg ctgatggggt cggaattggt
     1921 ccccacgaac tccccgttga caaataccct cgcgctactc ataccgacca cctacaggag
     1981 cgacctccag atccagaagc atcctcttca cgtcctcgta atcctcaacc cccgtcgaga
     2041 gctctacccc ctgggcgaag ttcttgacga ggccgcagtt cggcccctcc ggggtctcgc
     2101 tgggacagat ccggccccac tgggtggggt ggaggtccct cgcctcgaag tggggctggg
     2161 accgggagag gggggatatg accctccgga ggtggcttaa gctcgccatg tagtcggtcc
     2221 gatccaggag ctgggatacc cccgtcctgc cgccgaccca gttccccgtg gccaggggat
     2281 ggatcatcct ctcggtgagg acgtcggccc ggacggtggt gatgacgttc agctcccggt
     2341 tcctcatgct cgcccgctcg agctggtact tgatgtcccg cgtcagcctg ctgaaggcga
     2401 cccggaatag gtcctccatc aggtccccgg agagcttcag ccgtttattg gcgtagtggt
     2461 ccttgtcgtc ctcgccccgc ttccccaggg cgagctcgaa gcaggcctcc gccatcctcg
     2521 cgaggaagag ccctttcgcg taccggtccc cctcatcgac gccgatgtgg ggtaggaggt
     2581 accgatcgag gacgtaggcc gccctcttct tctggtactc cttcgcctgg ccggcggcga
     2641 cccggcctcc gatcttctcc agggcctcct ctttggtcgc cacctccgcc tcctccaggt
     2701 tctcgagcat gaatttgatg atctcggggt tctcggagac ggcctggacg atctccatat
     2761 ccgtctcaag gccgagggcc ctcgccaggg tgacgaagtt gagcctcccg ctgacggagg
     2821 ggaaggagac ctccagaatc gaccttcggc ctcgctcgac gatcaccaga gatcgatacc
     2881 cctgcctctg ggagaagacc ttcgccacct cgatgaagtc gtcgtacctc tggttgtact
     2941 ccaccaggat cttgttgggg gagaggtcct ccagggtcat gataaccctc tcggagccgt
     3001 tgacgacgaa gtatccgccg gggtcgaggg ggtcctcgcc gaactcgatc atctcgtcgc
     3061 gagacagccc cgacaggttg cagacctggg acatgatcat gatggggagc atccctatca
     3121 tcgcctccac cggctccttc tccgtctccc cctcgacgat cgtcatctcc agccggaggg
     3181 gggatgcata ggtgaggttt cgtaggcggg cgtcgttggg gtagagccgc tccaccgagc
     3241 cgtcggcctc ggtgaccgtg gggttctcca cccggatcga gccgagcttg acgtaggtct
     3301 cctcgatgtt cgtctcgatg atccccacct cgtcgatcac cttctggagg cccgagtcga
     3361 tgaaatcgtt gaaagagttt atgtgatggt gaaccagctt atctttggta aaataggcct
     3421 tgtatagaac ttctctgtcc agcaaacttt aaaacacccc ttaatcgatt accagcctgt
     3481 aaaagaccga ttcccccgac gtgggactat tcctcttgat cttcaccacg tcgcccacct
     3541 ttcctccgat ctccttcacc accggatcgg tggcgtggat cttcgggagc tgagcagaat
     3601 cgacgccata cgccctgaat acctcctccg cctcctcggg ggtgaggagg atgtgttccg
     3661 gcaccagatc gtgatcctgc agcgcaaact tcttcataca gccacttccc agaattatgc
     3721 gataagcggg ctcgaaggga tttgaaccct cgaccacctg gtatctccgt tcaaaaaact
     3781 taaaagccag acgctctgcc taactgagct acgagccccc atgagcagat cagcccatac
     3841 cactgttcaa gagatggtca tccggcataa aaccttttcg ccttccgatc agatctctcc
     3901 gaggacgatc gccgcgatgt cgtcgccgac ctccgaggtc gtggcgctcc cgcccatgtc
     3961 gtaggtcctg accttacccc gctttatgtt cgtctcgatc gccctcacca cgtcctcggc
     4021 ggcctcgcct tcgcccaagt gctccaggag catagccccc gcccagatgg tggcgatggg
     4081 gttgaccttg ttctgcccct tgtacttggg ggcagagccg tgnntcggtg atgatgtcgc
     4141 cgaacatgtt gggggcgacg acgacgtcga accactccgg attcttgaca aaccacatgg
     4201 tgatggcgtc gacgaagttg aagtcggtcg atacctccgg atactcggcc gcgagggcct
     4261 cgaactcctc cctccagaag ccgtagatat cggagaggac gttcgcctta tcgacgctgg
     4321 agaggtgctt tctccgctcc atggcgaggt cgaaggcgta cttgatcacc ctccgggtcc
     4381 cctccttcga gatcatccct atctggtagg cgatctcgtc gctctcggag tccacgtcca
     4441 ggccgaactt gacctggtac agggtccgga tcacctcgaa ctcggcgcgg ctcctccccg
     4501 cccgggcccg gcttccgatc ccgatgtaga agtcctcggt
//
LOCUS       DASM01000103           38070 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_13913, whole genome shotgun sequence.
ACCESSION   DASM01000103 DASM01000000
VERSION     DASM01000103.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 38070)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 38070)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..38070
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_13913"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    1893..1930
                     /estimated_length=38
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         7428 a        11400 c        12180 g         7022 t
ORIGIN      
        1 tcgggttctc cttcttcagc cactccatct acggcggcgg cggacctggc gtcttcaacg
       61 gcaaccacgt cgtgacgagg cactcccgag gattcgccat cccctgcgtg tccgcagcgg
      121 ttgctcttga cgccggaact cagatgttca ctccggagat gacctccggt ctggtgggag
      181 ccgtctatgg agagatcaag gagttccgcg agcctatcgt agcagttgcg gaggctgtat
      241 agataggttg agtatgcatg gtcactaaat cagacacgga accggtccag atagagatct
      301 tccctcaaag atttctaaag cccgatacgg cccagcgcct cctggaggag atatacagca
      361 ctggaggcat cctgcggatc atgatccaag gtcccaacct ccccaagtcg gtgccctacg
      421 gccccggcaa ggggatcccc atcgaggagc acttgaacat gctcctggag ctgggggatt
      481 cggccctgga gctgagggtg aaggtgggac ggatctggct ggagctggat agcgaggaga
      541 ggattgaagg ggtcaaagca gcatgcgagc gagtgctgcc ctgcaccttc agcatcaaga
      601 gaaagcaact atttcacacc aagcccaccg tatcggacta tgcgaagtac gggagcgagg
      661 tggaggataa gaggatcctt ggactggtcg atccgaaggc caagcgagaa cgcgacctgg
      721 ccattttgtc tgatacaggc ggtgacacat aaaattttca caaggaggtt aaggatatgg
      781 catacaaacc ccaatattat cccggcacca cgtctgtcgg caagaacaga cgcaagcaca
      841 tgtctggcga tctggagaag ctgagagaca tctccgacgc cgacgtcgta accatcatgg
      901 gccacagggc ccccggtgca gactacccca gcacccaccc gcccctggca gagatgggag
      961 agcccgactg tcccataagg cagatcgtaa cgcccacccc cggcgccgcc gctggagaca
     1021 ggatcaggta ctcccagttc gctgactcca tgtacaacgc ccccgccatc ccctacttca
     1081 ggagctactg ggcggccatc aactgcagag gctctgaccc cggcaccctc tccggccgac
     1141 agatcgtcga ggctagagag agagacattg agcagtacac caagatcatg atggacaccg
     1201 agatgtgcga ccccgctctc gccaccatga ggggctgcac cgtccacggc cactccctga
     1261 ggcttgacga agacggcatg atgttcgaca tgctcgccag gactgagctc gggtctgacg
     1321 gaaacgtcta ctacgtcaag gaccaggtcg gcatccccct ggacagaaag gtcaacgccg
     1381 gaaagccgat gagcgaggcc gaggcgaaga agaggaccac catcttcagg tgcgacaacg
     1441 tctccttcgg tggaccctgc accgcagaca agaggaccct cgacgagggg ctggtcactc
     1501 tgcaccacat gtgggagcgg agaagcaggt ggggattcag gccagagtaa atggtggtga
     1561 ttaaagatgg gaaagattca cactaaaaag ctgttcgtaa aggctttgaa caagaagttc
     1621 ggaagggact tcgacctgag cggaactaag accgtctacg agaggcttgg tcccgagcag
     1681 aacgcccgta agagagagtt catggaggcc tccaagaagc tggagggcaa gcgtggcatc
     1741 tccatgtaca acccctacct ccacgccggc ggcgtaccgc tgggccagag gcagctcgtc
     1801 ccctacaagc tctccaccac ggagtacatc gtagagggtg acgacctcca cttcgtcaac
     1861 aaccccgcca tgcagcagat gtgggacgac atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn tcctcaacca cgccatgccc ggcgccgccg tggttcagga gcacatggtc
     1981 gagacccacc ccgccctcgt cgacgactgt aacgtccgga tgttcaccgg cgacgacgac
     2041 ctggcggacg agatcgacga ccagtacctg atcgacgtca acaagctctt ccccgccgag
     2101 caggccgagg agatcaaggc agccatgggc aagacctcct ggcaggccat ccacatcccg
     2161 acgatcgtat ccaggacatg cgacggcggt accacctcca ggtggagcgc catgcagctc
     2221 tccatgacct tcatcgacgc ctacaacatg tgcgccggtg aggccgctgt ggccgaccta
     2281 gccttcgccg cgaagcacgc cgccgtcatc cagatgtccg acatgctccc cgcgaggagg
     2341 gcccgaggcc ccaacgagcc cggtggacta tccttcggct tcctcgccga catggtccag
     2401 acctccagga ctaagccctt tgacccgccc caggtctccc tgaacgtcgt cgccgccggc
     2461 gcgatgctct acgaccagat ctggctcggc agctacatgt ccggtggtgt cggcttcacc
     2521 cagtacgcca cggccgccta caccaacgac gtcctggacg acttctccta ctacgcctgc
     2581 gactacggcg tggacaagtt cggcgactgg ggcaaggctc ccgccaccct ggagacctcc
     2641 aaggacatcg ccaccgagac gaccctctac gccatggagc agtacgaggc cttcccgacc
     2701 ctgctggagg accacttcgg cggttcccag aggtctgcgg tcatggccgc cgccagcgcc
     2761 atcggctctg cctgcctgac cggaaacagc cagtctggcc tcgccgcctg gtacctcagc
     2821 cacctcatcc acaaggacgg ctggggcagg atgggcttct tcggatacga cctgcaggac
     2881 cagtgcggtc cgaccaacgt cttcagctac cagggtgacg aggggagccc cctcgagctg
     2941 agaggcgcga actatccgaa ctacgccatg aacgtcggcc accagggcga gtatgcaggc
     3001 atctccagcg ctgctcactc gggccgcttc gacgcctacg cgtgcaaccc cctgatcaag
     3061 gtctgcttcg caaacccggc cctgatcttc acctgggacg acatccgcaa gggattcggc
     3121 atgggtggtg cgagggagtt ccgcgcagcc ggcgagagat ccctggtcat gcccgcagtg
     3181 taggcgagag cctgcacacc ccttttttta tttgtttttc cgcaaggtcc attacataag
     3241 aattcttcta catgatccct cgttaatccc ccaggtgacg agctggtatt ataatggtga
     3301 atcttctggg gcggtctttc tggatggttc ccccaggggg ctcaaaaaga ggtcttctct
     3361 ggtcgttcgt ccccatccac ccgcgccccc atggggtggt gttcggctat cgatccgagg
     3421 ctttatccct ggtcagattc tggatcagcc cctcccccca ggggtgaaga tcgaccttga
     3481 ggtccctcag gaccttgggg gccttcaggg acggctcgtc gctcagcctc agggccacca
     3541 gatttttctt catccggagg ctctcttgaa cctcccattc gacccattcg cagtcctgga
     3601 aggtcttccc caccaggacg aagacgatcg atgacgcctc gatccttttc gtgatgcagc
     3661 gtcggacgta gacggcgttc tcgttcttga aatggacttt caatgcgaag tctatgaagt
     3721 cgaggcgggg gtcgaccccc ttggcgaagc tgcagaactc ccggacccgg tccaggtctc
     3781 ccgcccggaa gatgaggaag gccctcctcc gggggaggag ggcctgaggc ttgaatacct
     3841 ccccccgagc catctcctcc agcctctcca aagccgaggt tttcggctct gaagggcgga
     3901 tctctggatc ggcgatctca atcatccctt ccctccctcc ctccccagag ggtacgacgg
     3961 cctttcgggc ggtgcagctc ctgcagattc cgtttcttcc cgggtggcag cagaaggtct
     4021 cctcgatggg gaggccgtac cacctggcgg tgttgaggac gtcctccttc gtgaagttta
     4081 tgaggggggt gaggatggtg atgaagtcgc cgaaggccga gtttatggcg aagttggcgt
     4141 tgacgaggaa ctcctcggtc tggtcgggga accggcggct ctcatccgag aggagggcga
     4201 gggctacgat cttaatcccc cgctggtagg cgtaggaggc ggcggccatc agagccatca
     4261 ggtttctccc cgggaggaag gcgttgatgt agctcgcctt gcactccccc tggacgtcgg
     4321 actctatcga tctgcagtag ccgctcaggt ctatcctctc gggcggcgaa agttcgcacc
     4381 tctcaaagac gctcctgcag gcctcccact cctcatcgcc ggatatctgg ccgaagtcga
     4441 tgaagatggg gtgctgctcc tgcctctcgt tgttgaggat cttgcacatc accagggagt
     4501 ctatgtcacc ggagactatg gggactacgg ccatcatcga tagctcctta tctgagggac
     4561 ggcgcggttc tgcgcagtcg gactttgtac cttgaagcat caacttatat atcctcaccg
     4621 ggtggcccct cctcccagga gcattccgcc cgaagatcgg ggcgggagag atatcccctt
     4681 aatggtcggg taagccgaaa atggttcgca aatttcttat acaaaccgag cccaagttga
     4741 taatatgtcc agaaaagaga aggtcatatt cctctccgcc cataactccg cccgatctca
     4801 gatggctgag gggctcctcc gccacctcta ccccgaccgc tacctcgcct tcagcgccgg
     4861 ggtggacccc cgggaggtgg accccctggc ggtggaggcg atggaggaga gggggataga
     4921 catctcccgg cagaggtcga agagctcaaa ggagctttgg ggtacggccg tcgacgtggt
     4981 ggccaagatc cgcgacttcg attccgaggc ctgccccctc ttccccagcg ggaggtggag
     5041 ggcgttcttg cccctggagg acccctcgat ggtggaggac ccggaggagc ggaggcgggc
     5101 tctatcggcg gcgagggacc gcctggagcg gtggatcgtg gagaccttcg gagaagaggc
     5161 cttcaggaaa gatgatcagt gataggtatc agacgatccg gtccgtatat ttctccagaa
     5221 ggcccatcac cctcctcaca tccctcccct gagttcgcac ctcctgaagg aaggcggagt
     5281 aggtggcgtc gtcgactatt cgcggcaccg gcttgcagct catcccggtg agcatcaggt
     5341 tgaagcaccg ggcctccgcc tccgagatcc tcagggcccg atattctccg gcatgctccc
     5401 ggtagagggg gttatcctcc cggggcaggt ccagggcgat cttggcgagg ggagttcctg
     5461 gtataggctc tgcgacgctc gcgaagacgt cctccagcat cgcctcctcc atgaagtcta
     5521 gggtcgcatg gtagtcctcc tccatctcgc cggggtagcc gacgatgaag cttccgccga
     5581 ccttgacccc ggactggtgg gcgagctcca ccgcctccag gctcttctcg ggggtcgtcc
     5641 ccttcttcat cgccctgagg atcccggggc tccccgactc gatcccgaag aagacccagc
     5701 cgacggtgta cctcctcacc gcctccagga tctcctcgtc gacgaagtcg accctcatat
     5761 ccgggactga gaggttcttt ggacccagga tctcagcgag gccctttaag agggtgatga
     5821 aagcctcctt gttcacctcc ttcccgtagc cgaatagtga tcccgtaccg ccgctgaccg
     5881 ccaccctggt gacccccctc ctcttcatct ctcggacttc ggcgaggatg ttctcgaggg
     5941 accgggaccg gatcgaccgg ccgaagaacc gggggacctg gcagaaggtg cagcctccca
     6001 gacatccccg atgggtctcg atgtagacgt tggcgccccg gacgctctgg ctcctcaggt
     6061 cgtcggggat gaggggcatg acgtggtcga ggtcggagac ggggacggga tcggtcttga
     6121 ccaccacgcc gtcccggagg aaggctaggc cggggatctc ttcggagggc cccccttcga
     6181 ctaggtctgc gacgacctcc tccccctccc ccacgaccac gccgtcgacc tccagctccc
     6241 cgaggacgat ctcggggacg aggccgacgg ggcctccgac gtatatcctc ggagcccggc
     6301 tcgcgaactc ccggatctca gggtcgagga gctgggaggt ggagaagagg ctgaggaggg
     6361 tcagatcccc ctcggaggag aggtccctgg tgatcgagac ggcgtggccc cggtccctga
     6421 ggatgccgcc gaggacgagg gagccgtagg tgtagatctt cggggatacg atcgtcgtct
     6481 tcatcttaat ctccataccg ttattgatcc gtctcagacc cccaggccgt tgatctcatc
     6541 gatcagctcg gtgaaggcct ccacctccac ctcgatcacc tcttcggggg tcatgctgat
     6601 ggccatctct ccgtcgacgg tcagcctatc ggggccgcgc cggacgagcc ttctgacccc
     6661 gtccctcgat aggacccgtt tgatctcggg gtcgaaggcc atcgtcctcc ccgggaggat
     6721 caccgtctcc ttcaccgacg agaggtcgag ggcgaggagg tcctggatgg tgatgaggca
     6781 tccgatatcc ttttttacgg cgatgacgtt gacgccgtcg cccagcaccg cgaaaatcgc
     6841 ggagaggagg ggggcggcga cggagctggt gatgagggtc gcccccttct ggacttgggg
     6901 gagccttctt atcaggtcct tgtggtgggc cagggcgaag ggggacttcg tcacggggtc
     6961 ccagaggggg gttcccgcca ccctgaagct gtatttatcg ttcgtctcgg tcacgatatc
     7021 ccggaactcc tcgacggtgt gggggacgac cccggggatg atcggctcgt ttccgaggat
     7081 cagcccctgc tcccgagagt ttgcaaacct catcaggatc aggttcttga cccccatctc
     7141 ctccaggtcc cggcaggtcc ggtcgagctc ggggccgtcg ttcaccccgg ggatgaggac
     7201 ggaggcggcg tagacgtcgc agccttcagc gaaccgccgg aggttcgcca gggcggcctc
     7261 ggggtggcgg tcgttcatat gctctctccg gagggccggg tccgtcgaga agacggtgaa
     7321 ggagacctcc ttcacccccg cctcgatcag gtcctcggcc tcgtcgcccc tcgtgaagcc
     7381 cttgccgctg gtgtagccga ggtggatggg gaccccgccc tgccccacca tccgggccag
     7441 gggtaggagt tccggatagc agctgaggtc gccgcccccg ctgatcgtca ccttatcggg
     7501 cctcctcccg aaggcggctc cggccacctc catcgccacc gtcgccatcg gcttgaagcc
     7561 tgggtaagcc tcggcgatcg cccgggagca gtagtcgcac cccttctgaa aaggtaggca
     7621 cctcttgcac ccgaagggct tcagctcgtt tgtctttacg cccttgaagt agcagtagct
     7681 gcaaaatccc ttgcagtcta tccccggcct tccaccgacg tccgccagaa tctccatccg
     7741 caaactctcc agaccaggat cggcccggag gggtaaagcc tttgcgggat gggcgggtgc
     7801 ggacggagga ggaaggaatg gatgggatat tggataaaaa catcaaaaat tctggctaca
     7861 tttgaataaa tatagctctc cttgccaaaa gatttaaacc ctcttcagac tctatttcga
     7921 gatggaacca tttgaattga ggaattacat gagaaaaata tggacgattt tggcccccac
     7981 cgtcctggct atcgtgctcc tgatggggat ggtctcggcg accgataccg ccagcgtaac
     8041 cttgaccggc tgcctgctgg aggcggaata cgcgataaca accccccccg acggagccgt
     8101 ggatctgatc ttgctcaagg atgctactgc attggtctcc gacagcatgg gcgtcacggc
     8161 ggccgggcca tggaagctgc aggtcaagga ccagaaggcc acagatgtcg gttacatgat
     8221 gaagggttta acccccctct caagccccct cagcgtgagg gtcgatgaaa ccgcccagga
     8281 tctcacccag gagaggcaga tcgcaactgg cgatcctggc tgcaacgagc agactgtgaa
     8341 cttcgagtat cagcagccag taggtcaagc ggacggattg gggacctatt ccatatcgat
     8401 cacctacact ctgagctaca acgagtagtc ggcttctgga tgtcctttga gatcgatgga
     8461 tctggagggt cgatcccccc aatttttccc agttgaagat gggtgctctt atgaagaact
     8521 tatgggttaa aatctttgct ttcgcctctc gacgcgacgg aggggattta ctgagaatca
     8581 taaaaattaa gtgcataaaa tcatctgtta tggtcattct ggtcgcatcg atgttgatca
     8641 cgccgtccct gggcttcggc atcagcgggg cggtcttcaa agaggaggtc tccccaggcc
     8701 aaaagctcgt ccacgagatc acggtatcaa gcagcgagga cgatatgccg ttgaatatga
     8761 ccgcggaggt ctacgggttc gccatgaacc agggtggggc gaacatcgag ctttctcccg
     8821 aagaggacat cggctcaaac tcggccaggc ccttcctctc ggtggagccc aagagcttca
     8881 ccctggatcc cggagagcgg gagaggctcc ttctgacggg gaccgtaccc gaggacgtcg
     8941 gctccggagg taggtacgct ctggtcacca tcaccacggc tccggaggag gccggaagcg
     9001 tcatggtctc tacggcgatc caggttctcg tcctcctgac catcaaggat tctgaacttt
     9061 tcaggagcgg aaagatcacc gacctcgccg tctcgatgaa cgacgataac gtctcggtgg
     9121 atctcgtatt cgagaacatg gggaacgtcc actacaagcc cctcgtcgga gcggccctga
     9181 agggggatga tggtgaaatc ctggcgtatc tggagccgac ccagataaag agctcgatcc
     9241 tccccacgaa ctcccggctc tgcagggtgg agctggtttc cgatggcgac ctgctcccgg
     9301 gaaactacac tttggaggcg acggtgaccc ttgaagacgg tacggtgctg gacgtaagga
     9361 aaaccacctt ccaggtttga gggatgggcg aggttgcaag atgcgtaatc ggcgagaaaa
     9421 ccgtaagccg gaaaactatt agtaccttga agaagaccaa aaagatcgtg gctcataaaa
     9481 ggccgtttca cgtcatgatc atccccaccc tgggctgtcc atccagctgc agctactgct
     9541 ggtcctccga tgaaaggtcc ccaaggatga cgatcgagac ggtgaaggag ctggtccgct
     9601 ggctccggga gtttcgggac gaccccgtca ccttcacctt ccacggcggc gagcccctcc
     9661 tcgccggagc cgacttctac cgcgaggctc tggccctgat caccgagggg ctccggcccc
     9721 ggaagatcgc cttcgccctc cagacgaacc tctggaagat gaccccggag atggcctccg
     9781 tcctggcgga gtaccagatc cccatcggct caagcatcga cggcccgaag gatctgaacg
     9841 acctccagag gtcggcgggg tacttcgata agacgatggc cggctacgct gtcgcaaagg
     9901 agcacggcct ctccgtccag ttcatctgca ccttcacctc cacctcgata gacaaaaagg
     9961 aggagatatt caacttcttc ttggagaacg gcctcaccat gaagctccac cctgctcttc
    10021 cctccctgaa gagcgacgat ccggataagt gggccctttc tcccgagaag tacggcgagc
    10081 tcctggtcta cctcctcgac aagtacctgg agaatatgga taagatcgag gtccggaaca
    10141 tcaacgacct ctgtcgatgc gtcttcaccg gccgggggac ggtctgcacc tacgtcgact
    10201 gcatggagga caccttcgcc gtcggccccg acgggagcat atatccctgc taccggttcg
    10261 tggggatgcc ggagtgggtg atggggaaca tccgcgaccg tccctcccag gccgacctgg
    10321 cggggtccgg ggccggaagg aggatgcagg agttcaaggc gcgggtcgat ttggcctgca
    10381 agggctgccg ccacctccgg tactgccggg gcggatgccc ctacaacgcc atagccccct
    10441 ccggcggcga gcttttgggc gtcgatcccc actgcgtcgc ctacaagagg atcttcgacg
    10501 agatcgccga caggttcaat aaagagatct tcggctcctt cccgatgggg ccgacctgcc
    10561 tgcccaccga gccccctatg ggcggaaagc cggggatcac ggccctgatc cagaagatcg
    10621 tctcgagcta ggggtctgga tcctggaatc tctcctccct tccctcaggc cgcctcggga
    10681 tcggaccctc caaaggaacg atcgaccacc ggctgtctct tctctacagg atcgtcagga
    10741 tggcgatgac ctccaggagg tatatcgcga cgagcatgct tccctcaaac ctggttatcc
    10801 ggtttcccag gaccatgaag ccgaggagga ggctgatcag caccaccacg gggagggtga
    10861 cggagacgaa ggcctcgctc gcggccacgt cccccaccag ggaggcggct ccaaggatga
    10921 tcagggcgtt aaaggcgttc gcccccatga tgtcacccat ggcgatgtcc ccccggccgc
    10981 tcttggcggc gacgatggcg gtgctgatct cggggaggga ggttcctacc gccaggagga
    11041 ggaccgccac caccccctcg gggatcccca gggtcgagga gatcgacagg atcgcctcga
    11101 tcatgtagtt tgcgccgatg gccaggagga ggatgccggc gaagaagatc cccagctgcc
    11161 gtggattgaa cttctcgccc cccttcgcct cccccttccg gtgctcggag atctcgtggc
    11221 ggaggaagga gaggtatatg gcgatgagga ggagcccctc gatccaggtt atcctgtagt
    11281 cgaggaagac gacgtatatc gccagcatcg aggcgaggag gaaggagagg tccgtctcca
    11341 ggatcttcct gtcgatgtat atcccgtata gggccgccgc tgctccgagg gcgaggccgg
    11401 tgttggccag gatcgaaccc aggacgtttc cggcggcgat ctcgggggct ccgccgtcga
    11461 ccatcatcgc gacgatggag gtagagaact ccgggagggc ggtgccgaag gcgactactg
    11521 tcgccccgat gacgaactcg gagatcccca ggaccttggc gaagtggacg gcgctatcgg
    11581 tgatcgcctt ggctccgtag tagagggcga cgagagagac ggcaaaggtc aaaaactcca
    11641 gcaacgaacc agccccggaa gacccgtcgt cggtcctcat taattaatca atccgtcagc
    11701 ctctgccgcc aaagataggg gaagccaaaa tataaaccat tgaataggta ttttaatgag
    11761 aagatacaaa gtctccggcg atagatcgac cagggggtcc tcggggccgg tgcggctccg
    11821 aggtcgggcg gccggaaggt tgaagagatc tcccggaccg ggttggagta gcaaaggtgg
    11881 tcggggttgc acatcaagga gatagagctt gtaaacttca agtccttcgg caggagggcg
    11941 gtgatacccc taagaaacga cttcatcgcc atcaccggac ccaacggcag cggcaagtcc
    12001 aacgtagtcg acgctctcct cttcgcccta tccctctcct ccagcagggc gatgagggcc
    12061 gagaggctcc ccgacctcat ataccggggc gacaacggca agaaccccga cttcgcccag
    12121 gtgaccgtca agttcgacaa ctccgagcgg ggcgtccccg tcgacgaaga cgtcgtcgag
    12181 atatcgagga aggtcaggct gaccaagaac aagtaccaga gcatctacta ttttaacgga
    12241 aacccctgca cccagacgga gatccacgac cacctctccc gggcggggat cacccccgag
    12301 gggtacaacg tggtgatgca gggggacgtc accaggatca tcgagatgac ccccctggag
    12361 aggaggagga tcgtcgacga gatcgccggg gtcgccgagt tcgacgagaa gaagaagaag
    12421 gccctggagg agctggaggt ggtgagggag aggatcgacc ggggagacgt catcctggtg
    12481 gaggtggagg cccagctggc gaggctcgcc gaggagcggg atcgggccct cgcctaccag
    12541 gcccagaggg aggagaagag gcggatggag gcgttcctcc ttctggcgaa gctgaaggag
    12601 gcggagtccg atctggccga cctcgatacg gagctctccg acctccgatc aatcgattct
    12661 gacctcatcg ggaggctgga ccggggtagg gcggagctct cctccctgga ggagaatctg
    12721 aaggggctga acgacgagat cacccgcaag ggggaggacg agcagatcga ggtcaagcnn
    12781 ggtcaagcgg cggatggtgg aggtgaaggg ctcgatcaag gcggatgagg ggcggatcga
    12841 gatggccgat ggcgagatcg ccgccctgga ggcggagcag aggtccgact acctcgaggc
    12901 cgatcggctc ggccgggagg cggaggagct ctccatgcgg atccgggacg gctccatccg
    12961 gaaggcgagc atcctcgcgg agatccagga gcgggaagag gccctggcct cctatagaga
    13021 gaagatcgcc cgggccgacg cccggtacgc ggaggtgagg gaccggctcg tcgccgtcaa
    13081 gaaggaggtg gaggaggcga agtcgagggc agcggacctg atgagggaga gggaccgcca
    13141 cctcgacgcc gccaggaggc gttccatcga gcgggaggag gcggcgatcg agatctccga
    13201 ggcgagggag acgatggcgg ggctcgaccg ggagagggga cggatcggcc gggagcggca
    13261 ggagctggag gagaggatat cgatcttcga ggaggagagg gacgacctcg actccgcccg
    13321 gatatcgatc aggcgggaga tggcccgggt cgatgagacc ctccagcggc tccagatgga
    13381 gcgggcccgg gccgaggcga gcctccggtc cgcgggcgag ggcaccggct acagcagggc
    13441 ggtggaggcg atccggtccg ccatgagcca gggtcacctg acggggctct tcgggacgat
    13501 cgcggagcta ggggaggtcg gcgaggatta cgccaccgcc ctggaggtgg cggcgggggg
    13561 gaggctccag agcatcgtcg ccgagacgga cggggatgcg gcccgggcga tcgagagcct
    13621 caagaggtcg aaggcgggga gggccacctt tctccccctc aacaagatgg cctcgggcac
    13681 cccaccggcg ctgccgaagg agcccggggt cgtcgactac gccataaacc tcgtcgcctt
    13741 cgacccccgg ttcctccccg ccttctggca cgtattccga gatactctgg tggtgaagga
    13801 gctggatgtg gcccggaggc tgatgggccg ctaccggatg gtcaccctgg acggcgacct
    13861 ggtggagaag gggggagcca tgaccggggg taccttcaag tctcggctca agttcgccgc
    13921 cgacgagcgg cagaggctgg aggaggcgac gaggcgggtc ctcgccgccc aggcggagag
    13981 ggactccctg atcgagcggc tcgaccgggt cgagggggag gtctcctcgg tgcggagagg
    14041 ggtggaggag ctcggccggg aggtctcccg gatcgaggtc cggatcgagg agatcgacgg
    14101 ccagaaggtc cggctcgaga ggctcatcca ggaccgggag aggaggcttc tggagctgga
    14161 ggcggacgcc gccggggtga tggagcggct cgacctcctg gagcgggata tacgagagag
    14221 ggaggccgag actcgagggg ccgaggagcg ggcccggtcc ttggagaggg acctggaggg
    14281 gtcggagatc ccggagctat cggccctcgc cgacggcctg gaggccgaga tccggtccct
    14341 ctcccggaag gcctccgaga tcgaggccga gatcacaggc caccgcctcc gggaggagag
    14401 ccactcggcc cggcgggcgg agctctcggg gcggcgggag gcccgggagg agaggaagag
    14461 ggcggctatc gagaagaaga gcgccgccgc cgataggatc ggcaaggccg atctcgccct
    14521 cgccgacctc ggccagcggg agagggagat cgaggaggag ctatcggagc tgaaggggac
    14581 gaggagccgg ctcctcgacg aggtggtggc caagagcagg gaggttgact ccgccgaccg
    14641 ggagcgggaa cgggtccggg cccggatcgc ggcggcggag ggcgccgctg cggagatgag
    14701 gtcggcggcc gacggcctgc gggcggagat cgagagcttc gggatcgacg cctcggagga
    14761 gccacccaag agccagacca tcatcaggaa gatccacgcc ctggagaggt cgatggagga
    14821 gctggagccg gtgaacatgc tcgccatcga ggagtacgac cgggtcttcg accggcggga
    14881 gaccctccgg gaccggcggg agacccttca gcgggagagg gaagacctcc tggcgaagct
    14941 ggaccggtac gaggagatga agcgcgaggc cttcctggcc gccttcgaag ggatcaacga
    15001 aaacttccgg gataccttca atcagctctc agacggcgag ggggagctga tcctggagaa
    15061 cgaggaggac cccctcgcgg gcgggatgac gatccgggcg aggcccgccc ggaaggcctt
    15121 ccaccggctg gaggcgatgt ccgggggtga gaagagcctc accgccctct ccttcatctt
    15181 cgcgatccag aggttccgcc ccgccccctt ctacgccctc gacgagatcg acatgttcct
    15241 ggacggggcg aacgtcgaga gggtggcgaa gctgatcaag gggatcgccc aggccgccca
    15301 gttcatcgtc gtctctttgc gaaagccgat gatccaggag gctaagtacg tggtgggggt
    15361 gacgatgcag gagaacaaca tatccacggt cacgggggtg tgtctgaact gagcaccgta
    15421 gaagccctgg aggtccccga ggcctacggc ccggagccgg atccgatcat ggtcctgctg
    15481 gacctcgcga ggcgggggga gatcgacccc tgggacctgg acctctccgc ggtgacggag
    15541 aagtttctgg agcggatcga ggccctggga aatagagacc ttcccgccct cggcaggacc
    15601 ctcctctacg ccagcatcct cctgaggatg aagtccgact ctatggaggg gggggaggag
    15661 ccaggggagg agttcgagga gctcgacgac ccccccgtct tcaggaggcg gatcgtcgag
    15721 ggtctcccct cgccgccgat aaggaggagg gcgaggaggc ccgtcaccct cgaggagctg
    15781 atatccgagc tgaggcgggc ggagaccgtc gctgagagga aggtgggacg ggagagggcg
    15841 aggcccgagc cgacggtgga agaggcgatg gagcgggccc acgaggaggg gatcgaggag
    15901 aggatccgga ccctccgggg gacgatcttc gagatgctag cggcggagga acggatcttc
    15961 ttctcggacc ttccggagag ggacgtcctc agctacgtct ccctcctatt catggcgggg
    16021 aggagggaga tatggctcca gcaggacgcc ctcttcggcg agctttacat caggaggcac
    16081 cccggcgggc cgggggagca ggcggcgtga ggcgggacga ccttggcggg cgaagcttct
    16141 gaagatgggt ttgaggcgtc ggttgaggcc aggggggagg cggcggtggt ggaggctgcc
    16201 ctctttgcgg ccggaaggcc cctgacggcg gcggagatcg ccgagctgac ggacctgccg
    16261 ataagggtcg ccaggcgctt cgccgacggc ctcgtccggg agtacttcga gagggcgggc
    16321 gggatcgagg tccgggagtc ggaggggcgg tacgtcatgc aggtccgctc caccgtcgcc
    16381 gatcgggtga gcaaggtagg cccccaggag ctggatccac cgcttctgcg gaccctggct
    16441 gtcatcgccc gacaccagcc gatcccccag agcgagcttg tgagggtgag ggggaacaag
    16501 agctatggcc acgtccggga gctcctggag cgcgggctga tccgggccga gaaggagggg
    16561 aggacgaaga tcatcaccac caccaagggc tttgccgagt acttcggcct cgacttcgac
    16621 gaccccgact tcgtcaggag gtcgatggag gggcggaggc tcggggtcac ccccatgtac
    16681 cggagcctcg ccgaacggat gggcctggag tacgacgtca tcaacccgta cagccccggc
    16741 agaaatgacc tggtggcgat gaaggggctc gaccttctgg tcatcgcccc cggctacgcg
    16801 gagagggcga gggagcacta ctcgggggag ctcttggagg ccggggtgag gaccttcacc
    16861 cagctgaagg agagcgctgc tctcatcgcc gagaggtctg gggccgccga tcccgccagg
    16921 accgcatccc tcctcgagga gatcgatgcc ctcctggcgg gttacagaga gagggcaagg
    16981 ggcgcagggc cgatcaggcc cctatcgcct atggtcgagg agatagccct ggatctggga
    17041 attcccaccg ccgagaacgg cgtatccgtc gcccccgact acaagggctt ggaggcgaag
    17101 atccaggtac cgacccacca gccctacgat atggacatcc tcgagaggat caaagaaagg
    17161 tacgaggcga tcttgaaggg gctcgcggag agctgaggct ggtgaacgga tgctcctcgg
    17221 gcggattcca catagcgccc ttagtggatc gatggcggtc tcctcatgca gtggcagatc
    17281 tttttatctg gacgaaaagt gattaaaact cttgaccacc tcttcgcttc cggatggatg
    17341 aagagacttt agacgatctg gcccctggtt tcgcggatac gttaggtcct tcaaggccgg
    17401 cgaccccgaa gaggacagaa acatcctcct gaaggaggag cacaccgccc gcgtccgccg
    17461 ggagatccgc gatataggaa ggacgctcgg cctcgccgac gacgacctcc gcctggcgga
    17521 ggcgatggcc ctattccacg acctcggccg cttcccccag tacaccacct acggaacctt
    17581 ctcggaccgc cgatcctgcg accacgccgc cctatccgtc agggtcctca tcgatagcgg
    17641 tgtcctggac gccctcgatc ccgaagagcg ggagctgata ctcaaggcga tatcccgcca
    17701 caaccgggca ggccttccgg aggaggaatc ggaccgatgc ctcctcttct caaagctcct
    17761 ccgggacgcc gataaattgg acatctgggc cgtcctcctc gattactatc agcggcgcga
    17821 gacggatggg tatcggaacg gggccctgga gctggaccta cccgacgagc ccgggatcag
    17881 cgaggaggtc cgtcgcgaca tcgcagccgg agagatcgtg aagctctccg gcgtcaaacg
    17941 gctcaacgac ttcaagctcc tccaggcgag ctgggtcttc gacctgaact attggccagc
    18001 cctgctggcg gtgaggaaga ggggctacct cgaggaggcc cggaagtttc ttccccggac
    18061 cgaggaagta gatatgatct tcaccctcct aaaatccagc ctcgattgga ggatcgaagc
    18121 cgaggtcgct ggcgccgggg atggggcttg atggacggat gagatctcag ccggcctggc
    18181 cgagctcaca aagtctctct ttttttccga gggcttcaag atactccagg accgccagga
    18241 cgaaggcggc gtgggaccac atcaggggca gcaccgacct cgggctgccg tcgtcggcga
    18301 cctgctccgg caacagcccc gtcgtcgggt gggcccgatc cgcgcaccag cggatcagct
    18361 ccagggcccg atcgagactc cccaccgcgg agtgccactg ggcgagccat aaggtggaga
    18421 tgatccagct gttcatcctc ccgccgtact cgtccccctc gtaccgggcc acgccccccc
    18481 ccggcctcaa cagctccctc tccaccgcct ccatcgtcct ggccaccctg gggtcgttgg
    18541 ggggtagaac cccaaaccac cataccccaa ataaggagga gtctacggtc ccgtcctccc
    18601 cagaccgcct gaacctcccg agccgatcgt cgtaaagccg gtcgacgatc gccgccctga
    18661 ccctctcagc ccccgcggac caggcctcga agagccggtc gtttcctagg gcgagggcca
    18721 gctccgccgc cccccggagg ccggcgtaga cggtggaggc gctgtaggtg aagactccct
    18781 tcctctcctc ccagaggtcg aagctcgccc gcggaaggcc ggagtcgtcc aggctccgga
    18841 ggagccagtg ggccgcggga gctgcgatgg agccgaagta tctgcccgcg gtccagacgt
    18901 tcctcgcgac gagccagttg aagtagaggg cgtatagggg caatcccgtc tcgtcgatct
    18961 ggatcatggg gacggggtgc caggtggacc cgaagtcccc ggcgggggtg tacttgtgga
    19021 ggaagtaccc ccgatcggtg atggtcttcg agaagaagcc gaagacctcg cagctgaggt
    19081 ggtggtaccg cgcccggtcg agggctatgg aggcccaggc ggcgtccctg ggccagcagt
    19141 aggaatagac gtcggctccg aactgccgga tatcggagtc gcaggaggcg atcgccgagc
    19201 cgttgatgtc catatgggcg acggtggcga tcaggctccg gtaaaagatc acgcggaccc
    19261 tctcggggag gctatccatc agggcggcct cggggaggat ggaggtcctc tcgaggaagg
    19321 agttccagaa gttcaatgta gtcctgaaga ggctatctcc ctcccctcgc ccgatctgcc
    19381 ggtggaggga gatgacctcc tggtagctct tcccgaagat ggtccagagt tggacctgtc
    19441 gagattcgcc ggagaggagg ccggatatgg tccaggccag ggtcgagtcc accgatccgt
    19501 gggctacggg gtttccgctg agcctctccc cctccaggat ctccttccag gtcccttcga
    19561 gccccctcca ctccgcggta ccggtggcga actggtcaaa gttcgggcgg cttccgtgga
    19621 ggatgtaccg atcgcgcttg tagtggatca ggacgtcccg gtccaccacc gccgtctcgc
    19681 cgatcttgct ctccaggatc ctgtagttct ggttggagaa gagcctcagg ttcctctcct
    19741 ccccggagac gttcttcacc tcgaagatcc tggagaaggc gttgagggag gggtggactg
    19801 cgtccaggac ggccacctcc agacctcccc ggggatctct gaagatcgtc tctccgatgc
    19861 tcgagacgat gtcgtggacg aagccctccc catcgggcct cctccccttc cccgggatgg
    19921 gagatcccct caagaggtcg gcccggaagc tcgactggta cctctggacc gggctccagc
    19981 cttcaaacca gctcgatcgg cgtggtgggt ccaggtcgca gatcccggcc ctgacggagt
    20041 ggccgtggtt ctcaaggccg acgtggggga agtagacgtc gcggaccgtc cccctctcgt
    20101 cctcgcagat gagaattctg ccgtttccca ggatcagagg tcgagtcatc cctatccctc
    20161 cgggtgcggg atggtatttc ggcgaccgac gtattttaac ctgctctcct ccgctctcgt
    20221 ctcagatcct aaggccggga tgattttaag atcaggcctg gccagcttaa caggtacttg
    20281 aaaataaatg ttttatatca gtaagttgta atgactcctt cagggagatt gttagcatga
    20341 aggcgattaa actatcgatg gccgtcctga tcatcggcct attcttggcc tctccgtccc
    20401 tcggagtggc gtcgtcgccc ttcggagagg ggtggcccct ctggaacgat ggggtgggga
    20461 tcggcgactc gcaggtcaag ggcatctcca aggcggatga gaccacttat caagagaacg
    20521 ctttgacagc agacgttttg aaggatgcac ctctggcaga ggaggcctct gccgatgcag
    20581 agtcgtcgga gggatatgat gccgtcgtcg acgactggat catcgacgag agcttctaca
    20641 gtgacagctc cggatacgag ggggaagagt tcggatgggg ggacaccatc gaccatcctg
    20701 ggatgccggg gcccgatgag aacgccctct ggatagtatt tccctacagc accaacgtcc
    20761 ggaccaccaa gctcgtcatc cagaggggga ggttcgcgaa ggagctgata attccaggga
    20821 tggacgggaa gctaacgatc catgaggtga ggcccgacgg caccgaggat acttacgtcc
    20881 ccgactggca ggtgaaggcg agaagggcct atcgggtctg gttcaccgcc gactccgtcg
    20941 gcgactacac cgtctggtac gaggtccaga acccgaagac gatggtcacc accaagagca
    21001 acgagatcaa gtaccgggtc ttcgagaacc tggccatcgt cgtccccaac gaggtcaaat
    21061 gtgaggggga gaccgccacc ctgagggccc tggtctcggg atgctacgac ccgatatacc
    21121 agtggttcaa ggggatcacc tccgaggatg ggacctcgat ccccggggct acggggagcg
    21181 tttacatcat aaggaacgtc tctcctgagg acgagggcta ctacacctgc aaggtcacct
    21241 gcaacggaaa ctccgatgag gatgcgggat atctccgggt ctgcaccagg gatgaagaag
    21301 ggaacaaatg caaaggccga tgatccggcg gccgacccgt ttaaatgggc gcgggccatg
    21361 acctgaatca ggtcatggca acctcttttt cttaacccat ctaagaccgg cttcagccga
    21421 actggagctt gcaatccacg gatgcctcca ccatgtacgc ctctcccggg atcaaaggct
    21481 cgctcctatc gaggtggacc caccggccgt ccctgtagct ccagaggaag gcagagacga
    21541 tcccctcctg gacggcctgg gggtagtcgt atcgcttgtg gttcgccctg acggtgacgt
    21601 tcgccagaga gaccgtctcg ttcgtgggga cccccaccag gttccacccc tccctcagat
    21661 cgaccatgta ggggaggtcc acgggctttc cggctatctc caggttgaag ctctcgcggc
    21721 cgtcgatgac gtaacccctt cccggctcca ggtcggtgat gttggcggcg tggtcctcct
    21781 ggatggtgta gttccagccg tccccggcga gggtgaatat tccccggtag ctcttcctat
    21841 tcaggagctt ggagatggag gggtccttcg gtttcaggtg gagggagaag ggggtccacc
    21901 ccaccgagac gttgacgacc tggaccattc ccgggacgat ccggtactcg acggtgaagg
    21961 tcctctcctc ccccagggcg gggctcagaa ctggccagac cacccgcttc tccgagccta
    22021 ccagctcctc gccgtgccat accgccctcc ccctctcctt caccacaatc tcctcggcgt
    22081 ccctgttgag gaacacctcg ccgatgaggg cggggggagc gttcaggctg aggtagccgt
    22141 cggaggagtc ctccataatc cagtagatcc agtctccgga ccaatccgaa gtcggcgtga
    22201 tcgataccgt gacgtcattc gattcgttcg attcggtgac gttcgaagtc tcgacctcgt
    22261 aggggaggtc cggggtcttc gggatcgtcg gccggaaggc gggatctccg aagagggtga
    22321 agatgctcgc cgtctggttg agcatctctt cgatggagag gtaccagggc tgggattcgt
    22381 ctatcttttc gaggtcgatc ccctcggtgg cctggatctt cgatgcgtaa aggttgtttg
    22441 ccgccatcat cgcctcgccg atggtggcgt tctcaaagac cagggcctga tgcatcctcc
    22501 ccacatagtc gtcggatacg aagatccagg agttggcgct cggtcccgcg tagttcacag
    22561 cccccgcctt caggaaggcc agggccatgg agtcctcgag cctcgtcggt atgtatacgt
    22621 cgatcccatc cctgtaggaa aaggtctggc ccttgagctt ggtggtgagg caggcggagg
    22681 tggtggtggt ctgaggggcg agggtcaact gcttgacctg ctcctcgtcc aaaagctggt
    22741 ccatcaaagc ccagatcgag agctgccatc cccagtcgac gccgtgatag tcgaagtaca
    22801 ccaggctctg gccgctgttc atcttcgatg agaccctctg gtaggtcgcc tcctcccagc
    22861 ggaggttctc ggcgttcagc cccgcagccc tcaggtactc tccgatccgg aggggtatcg
    22921 gcgactgagg ccacgtcgag gggtaatcgg cgggagagga tatcaccagg gcggagcctc
    22981 gccattcgcc tccgatccca tcgtaggcga aggttctggc taccatctgg gaggcgtcgt
    23041 agacgctgag gcctatgatc ctgcccgtcg ccacgtcctg atagccgtcg tcgtcgagct
    23101 cgatactgta ctcacggcat acatcgtact cgaagacccc tgccgcctgt ctcatccccg
    23161 ttgatatgaa ggggagggac ccggggcccc ccaccaccat caggtacctc ggcgatatct
    23221 ccaggtcttc caccagggcc tggaggtccc gctccaggac cgatggattg aggctctggg
    23281 ggtcggtcgc cgcatcgaag acgatccccc tcctagcccc ggccatctgg gctgatagga
    23341 gagatagctt cgggatcttg gtgtggctga aggcggtgac gtttatgggg ctcgcagatg
    23401 tggcgacggt gacggtgaag ttctcggtcc tgtgatccac cgtctcagag gcggtccagg
    23461 cctcgaggtt gaaggtccag gtaccgggcc tccccaggag gacggtggcg ttcgagacct
    23521 tcccggggcg agagtcggtg aagacctggc tcgacttctc gccctcgggg ttgtagtagt
    23581 tgaggacgac ccgggccggc tgcctcgtca cctcccagga gatcgtggtc catcccggcg
    23641 aggcttttgt aggatgggtt atgttgatga agggaccgtc gtcgatcctc gccctctccc
    23701 ttccgtatcc accttcctcc tcctcctcct ctttgttgcc ggtgtagttg acggagatcc
    23761 cgccgtccaa agcctgggtc catccggcgt cgagggcgtg gaggatcagc acctccaggt
    23821 tgagggtggc gaactcccgg ggacccagcc gatcgatcgt ccagctcagg ttcgtgccgc
    23881 tgaagaaggc gctgtcgggg gtaggggaga gccccgtcga ggggtcggtg aggacgacct
    23941 tccctgaggc ggagctccac caggccacca tccgagcggg gaggatcagg tcggtcagag
    24001 ccaaatcctt cagctcatct tttccgctgt tcttcagggt tatcttgagg cggacgatct
    24061 ctccctcgcg gtcagagggt atctcggtgg ggttcacctc cacctccagg accagatctt
    24121 cgccgatctt cgtcctcctg aggtccgacc ttggcttcgg ggagaggagg gatatgtctc
    24181 gggagttggt gaggaccacc gactccacct cgtcgccccg ggccctcatc tcctggatca
    24241 gaagcttgtt atccccatcg atgaccgtca ggttcatctc ggcgaggcgg gacctgacgc
    24301 cttcggatat ggggcccgct gatacgaccg tcgtcgatcc gagccgggcc aggtcgtcct
    24361 cggtggagtt agagagggag tccccgacga agaggacggg ccagttgagg taggatgcga
    24421 tggcggctgc ggtgaggcct gcgctgtagt tgtcgccgac caggaccgct cccggcgact
    24481 catcccactt cgctgcgacc ttcgaggcca tctcgatgct gtcgttgtac tcgatgacgc
    24541 cgtcggggtc ccagaagacc tccccaatcc ccagggtcct accctggagc cggtccatca
    24601 gccactggac cgaagcggag gggggctgga gctccgacca gaggggggtg tagacgacgt
    24661 cgtactcgaa gaccatccct gaggccccct ccttcgccag atcttcgatg cggcggacgg
    24721 tcccttccac gttctgggtg gatatggtgt actcgatgta gtcgtcgctg agcctgacga
    24781 agacgggagt ccccgccatc gacgatgcca ccttcatatc cgccggctcg actgggctca
    24841 ccaccacgaa gtgggacctg tccgatatct ccgccaggtt gtacccctcg ctccggtcaa
    24901 aggggtcgcc gatggccacc cccagcttcg cggggccgag ctccccgacc atccgtctaa
    24961 cctcgtcgac gacctccagg acctgctcct ccttccatcg gctccatagc tggcggttgt
    25021 agctgctgga acctaccctg gagaggtcga cgccggtatc gttgtagaac tcctctttgc
    25081 atacgtcgca gaagcaatgg ttctcgtcca ggaatccgaa tttgtagagg acgactccgt
    25141 cggcgccgta atcatcgatc acctcctcga cccggtcgta gaggtgatct ctcgcatcag
    25201 gatctatggg acagagccta tcggcgacga cgaccccctg ggataggatg gcgatctggg
    25261 ccgagtcggg gtaaaggtct ctgtactgcg gggggatcag gccggatatg ccagcagcct
    25321 tcgtctcttg gtcccccaga agcatcttcc ccaggaggct gagggctgct cctctctcct
    25381 gcgccgccag gacctcctcc tcggagacgc ccgccttggc ctcctcaggg acctccaggg
    25441 tggcggtgac gtaggcctcg atcccctcct tctgggctgc cttaaccagg ctcttggcga
    25501 ggtccccctc ggcgtcgatc accagggctg agatgttcgc cgccgccatg gcgtggaggg
    25561 cggaggtcgg accgtaccta tcgaggtcag ccctgtcgat ccatccgatc ctatccttag
    25621 cctggatctc ccgcggcatg acgaggaggg gcctcgtcac gacgccgccc tcctccgacc
    25681 atacggccag gggcgtcgcg gcgacggtgg cgcgccagtc gtcagccccc accaggatga
    25741 tctcctccaa ggaatccgat ttcgtccccc tctccatctg gccgtcggcg gcgatcgcca
    25801 ggatcgcaaa caacgtcatg accgtcaaaa agcctctcat tcatcccacc tcctcgatgg
    25861 tcagccccgc ccccctcagc gcctcgatgg tggcggtatc agcccccgtc ccccagatga
    25921 ggaccctgga gaggcccgaa cccaccatct cttccagggc cgcggcgctc gcctcggtca
    25981 ggggagtgcc gcagattatc aagggtgccc cgatcctctt cgcctcctcg tacgcggcga
    26041 aggcctccat ctcgtcgtct ccggcgatca ccaccgcctc cgtccccgcc ggcgagaagg
    26101 ccgaggcgaa gagccacgac gtctcgcaga tctcgtcccc cgcgatcctc tccgtcggca
    26161 tcatccccga aataatcctc tccacctcgg aggagacgac ggactcgttg cccaggatca
    26221 ccaccttcgc caggggcagc tcctccagca gctggaggtc ctcctccgat agatcgccac
    26281 cctggaggga tatgacgggg accgtatcgt ttgccagccc ctcggcgtag gcgagggcga
    26341 tggcccggta taggaggcct tccccggtga cgatggcgaa cctggcggat tccaccgcca
    26401 gaaactcagc cctctccgtc ggcaggggta tgttgtagga gtcgtggagg aggaactcta
    26461 tcctgtacct gccgggcctg gtggccagaa gggtgaactc gcctatcccg gacttctcct
    26521 ctccgacgga tatcacctgg ctttttggca tcaccaggac ctcgggaggc gcagccacct
    26581 ggatcgatat cctctcgtcg acgttcagct tgattccgat cgacccgatg tgggtcctgt
    26641 tggtcaccac caccgctggg aggtcgacga tcgcctcata ttgtcggacc tcctggttca
    26701 gctcgatcat tgccgtcacc gtggcgttct tcagggggtc gccgagggag gtcgcctgaa
    26761 tcccgatcgt ccgggtatct ccaggcctct cgcccacgat ctccaggccg aggacccggc
    26821 tcacccccga ggagagggtg aggttgtagg gcatctccac cgtctcggtc ccgtcgaaga
    26881 gggtcgccgt ccatcggggg gggaggcggg ggacgtccaa ggatattacg tccttgaagc
    26941 ggccagcgtt cctgacgaag atctcaaact cagccggctc atccgggtct gcggtcgcgg
    27001 aggatgatct ggcatcgtct atgctgaggc tcaccccata gctctcctcc tcgatcaccg
    27061 ccacctcgaa ctccaccaga agaggcaggg aagatgcatc gtcgaagaag taggcgaaac
    27121 caggatacgt tccagggctg atctccggcg gcgtcgagaa ctttatcttt accttgacct
    27181 cctcccccgg ggcgaggatc tcgaacttcg agatctccat caccaccagc tctttcgcct
    27241 tgccggccac gggcatcgtg gtgatcccga agatgggctg gtttccggag ttcttgagga
    27301 tcagggtcct ttccgtaatc tcaccgggat ggagttcaac ttcgaaccgg tctatatctg
    27361 ctgccagctc ggccgcagaa acggcgacca gcagcaacgg gatggcgagc aggcttataa
    27421 gcttcatctg ggcacctcac tcgggtcatc gcggatcggt gggatctctc acgataccgc
    27481 cttcgtccca tcggcctggg gaggggctga aaaaccccct ctccagagtg agactggatt
    27541 gtggtcctga tcggcgccat ctgcggatag ggtccggcgg gtgccgggcc gggaacgccg
    27601 gtgagagctc actggtctta cgcgccttag gttggacgac ctcgacgatt ccgatttcag
    27661 atacaaatca attatatgcg caggttattt agacgaacta tggatgaata agctttgcgg
    27721 ccaacttcct gccctgggtt cggccttttc gccgccgtct taagggctcc agtcggggga
    27781 gagggtcgcg aaattctgat tccaaagctg tcccaactct gggacggcca tgataagtta
    27841 taaatggacg gcgcataata ctaatattgt caaccaaaaa tgggctatgg gcgcttatga
    27901 agaggggttt caccttctag ggagttgggc agtccccagg gcccatgggc agatcgtgag
    27961 gtggaacgat tggataaatt tacagcagct ggattcgtct tcgcctttgc tgtcatcatc
    28021 tcgatggcac cgacatcggc ggcacagccg atattctcag agccctatca gtatccggga
    28081 tacgatccct ggtcgtcgat ggacccctgg cctggctctt gggacggtag ctggtcgccc
    28141 atgacggggt acggctggcc ataccagttg gaggatccct tctggacggc ggaggagaac
    28201 ccgtggacct gggggtcgtc gggggtctcc tcggatggag cgcccactgc taccaggccc
    28261 agcggctatc agccttccag tgcgccatcc gcctcgacgg ggtcgccgat atcccacctc
    28321 gccgatggca tctccctatc agagcaggag gtgagcagcc ttcccggcgg ttggagcctc
    28381 ctcgctcctg ggggctcagg ctcgccggtg gcttcgggga ggccggaggt cttccagggc
    28441 tattacggag gaacccaggc tttccggaga tacagcggcg gcctgcagca ttggatctac
    28501 tactgcggga ggtggaccta cggccccgca gccctatggt acggtcagca gactaacacc
    28561 gtcataagaa atgatcagta tcagatgatc tggtcttacg agagataccc tagcggctat
    28621 gaggcgtggc agtactgggg atactggtat ccgggctacc gtcacgcctg gttcggggct
    28681 gatgccaaag gctggcatca gattgcggta tggggaagcc gctcaggatg gagcaacccg
    28741 atctgggtct acgtctggta aatccgagac ggggctgaga tcgcgatgcc ggagaagatt
    28801 gacctgagat cacaagaccg gaaaagccgg ggctgagatc gcaagacagg aggagatggg
    28861 ccatctatct ggcgggggat ccggatctcg gcatcgacga gaagcgccgg atagaaggcc
    28921 ggattaatcc gtctctcgtc tttctccgtc tctccgtttt tttaaacgcg gacgtctcgg
    28981 tcgggttttg catatccata ccgttgatcg acatctattt ctggcaaaga gaagagcaga
    29041 ggccccatgg cgatgaggac tttctctggg gatggtgagg cccatggacc gtgagcgggc
    29101 ctatctggtg gccaccccct tcaagaagcg ggacaaaaaa agcctgaaga taagcgactt
    29161 cgtcttcgcc ctatctctgg acctgaaatg gggatccccg gagaaggtga gggggcttct
    29221 tcaggaggcg gcccaggagg ggctggtccg gatcgagggg gactacgtcc acgccgcctt
    29281 cgaggagggc caggccgagg ttcccctcgg cttcagccct tcaaaagagg aggacatctt
    29341 tgagaggagc gttcgcctca tcgtctccgc tacggggatg gagagacggg aggtgatctc
    29401 catggtgaat gagaagcagg actcccttca gggactggta aacctggacg cggtggcctt
    29461 gctggtggca agagagatgg gggtggaggt gagagagctg gcgacggagg cctaccagaa
    29521 cctgatccag gagggaaagc cgcgggtggt cacgacctga tggcatcttc accgatcgga
    29581 gacggacttt ttttgcgggg cggttggggg gcgggaaggc aaaggattta tcccgaagcc
    29641 gcccctgatc tctggggcga gtccattggg tttcaggtcg ctttttggag ctgggactta
    29701 tccggtcccg actcattatc cggttcccac ttatctggtt ctatctctct ttctgtccgc
    29761 ctgcgccttt ccggccgggg ccgttgagga ggccaaggtc gaggccgggg caggggcaga
    29821 ggcagggacg cctggagcgc tcttccggct cgaagacggg gctgtggtca tcgactggtc
    29881 gcctggtctc ggctttcccc gcaactccac cgtcttcctc gagaattcga gcctggcggg
    29941 gatgggaaac cagagcctcg acgccgggct ggcccacccc ggagcggaga acggatcgcc
    30001 ggatcagaac gcgaccagtt tgcctttcag cctcgaggag accggactct tcggcggtcc
    30061 atcgaccggc tccggcccgg gcttatcttc ccgggcaggc ggcagcggtc gccccgcgga
    30121 gtacggcagg agggtcacgg accttgtggg cgtcttctcc atcgatatga ccataaggct
    30181 gggatctaac ctgagctcct ctccggggac cgcagaatgg atcccctgcc cctgagggcg
    30241 gcgtttactg ttcttctatc ctccaggggc taccttctcc ttcgttgggc gatggtggcg
    30301 ctgaggtgtg ccccgtaaag gatgatcatc ccgctcaggt agacccagag catcagggcc
    30361 accaccgtcc ccagcgagcc gtagaccagc cgatttcttg caagccatat cgccaggtac
    30421 caggagaagg ccatcttgct gaccgtccag cccaaggtgg cgaccagggc gcccttcgcc
    30481 gcctcgtcca gcttgacctc ggtgttgggg gcccagagat agagggcgat ggagatgaaa
    30541 aagaccagga tgaccggggc gaggtttgag agggctccca aaaaccaggc ctggtacagg
    30601 ggcgcgctgc cgatggagat ccttgataga aggccaaagt accccgccag aaatagggag
    30661 acgaccagaa gcaccgtcat cagccccccg gccatggcca ggccgatgag ccgccatctc
    30721 aggtagttcc tccgggccgc ccctctccag gcccgatcca cgttcagggc cagggcggtg
    30781 aatacggagg tggaggacca gaggaggccg gccgccgcca ggatccccac cggcaccctc
    30841 agcatcatca tctgggcgat gttctccttc agcatatcct gagccgacgg gaggcgacca
    30901 agctccatga tccgctggag gctctccctg gccagctcct ctcccggagg aagaacctcc
    30961 gtcaccaggt tcaaaatctg gatctggacc tcctgactct ggaggagggg gctggccagc
    31021 accaccagga tgatcagcag cggaaatagg gagaagagag cgtagtaggc gatccccgcc
    31081 gccgcctccg atgcccgggc cgcgttgaag cttcgcagag cttccaccga caggctaaga
    31141 gctccgccgc atagaacgtc ggcccggacg aagagggacc cggcccggcc cctgatccag
    31201 tcccgggtct tgtgccacca tccgccgtct tcagatttcg tctcctccaa gagatcacca
    31261 tcagggatcg tggatttcgg cagatcctct ggacgccggg atagaaatat ttccatcccg
    31321 gactccctcc gaccgcttac tttaaggtct tatgcgacca tcgtttatcc ggggaatggt
    31381 atgaaggctg tggcgattct attaggtacg ttcattctgg ccctcctggg gggtgctgca
    31441 gacgcctcga cgctgaccgt ggtcggcgag ggggtctttc aggtgccggc ggacgtcgtt
    31501 tacgtctcca tccgcgcgac cgccgaggat gataacttat ccctggcatc gatgaactcc
    31561 tctgaaatcc tgaaccggac cacagaggcc ctgatcgaag ccggcgtgga ccccgaagac
    31621 ttctcggccg ggcggggaag gagcgcgtcg cggatccaga ccaccagccg ggtatataac
    31681 aactccacgg tggtggcggt ggcagacgag gcggtgagca gggtcaccga agaggtcacc
    31741 atcaggttcg atgcccaaga cgaggctctg atcaacataa cccttgaggt tgcgcgagcc
    31801 gagggagctg atgcctccat atccggatac ggtcttgagg atgtggacga ggcgtttgcc
    31861 caggcgcggc agagggccgt tgaagacgcg gagatcgaag ctgaggctct cgcctccgct
    31921 gcggggctga agctgggaaa gcggctggag atcttcgagc ccgcaagccc agaggtgagc
    31981 cagccgaccg aggcctatga tccgctggcc ctgggcatga tctctctctt cgacttcacc
    32041 tggccactga ccccctattc cagcgacccc ttcagctcct cgagcccctg ggatacagcg
    32101 acgcctgcag aaccggggat gctggaggtc agatctcagg tggtggtcac ctatgaggtc
    32161 acctcgtaac ggtgaggtcg catggtgacg atgaggtcgc atggtgaagg gccacggtga
    32221 ggatccgatc ccgtctccca ctattttcat cgcccaatat tttgtccgca attttcgtcg
    32281 atttctgtaa tttccagccg gatccatctt ctgtcgcaga gtacttttga aacataattt
    32341 acttatccta ttaaatcaaa tgaaacatgg tgatccaaat gagatggctg aaggctctgc
    32401 ttcttacatt gatcatggtc acggctccag gcgggatggg gttggagctc attggtgatg
    32461 atgtggtatc gatcgactcc ccggtggagg atgacatatt cgcaggaggc gacactgtaa
    32521 acatcaacgc tccggtggat agcgccgtca tcttcggggg gagggtgaac gtggacgccc
    32581 ccataaaggg cgatctgatc gtcgccgggg gggaggtcga cctgaactct gaggtgggcg
    32641 ggaaggtcct ggcggcaggg ggcaccatca acctcagggg cgacgtggag aggaacgtgg
    32701 tgatggccgg aggcacggtc cgaatactat ccacgtcgaa tatcgggatg gacgccgcca
    32761 tctccggagg cgaggtttac aacgccggaa acgtgacggg aaccctctgg gtcaacgccg
    32821 gagacttcga gaatacaggc aacgctggcg aggtgaagta ccagatgtgg gaggaggatg
    32881 aggctgcccg ggagccggag gccctgatat cgaccttcgg catcctgatg atcctcggct
    32941 ttctcatcgt cggcgttctg atcctgaggc tctttccaga aagcatgtcc gccgtggatg
    33001 aggagatcag gagatctccg ccgatcaggg cgatcctggg cctcgggctg atggtggcat
    33061 cgatggttct gatcttcgtc tccatggtca ccgtcgtcgg aatgcccctg ggttttctgc
    33121 tcctggccct cttcgtcgcc gctctgctgg tcgcaaacct cttcgtctcc ttcagcctgg
    33181 gcaggtggat cgccagagca ttgaaccgga acctgggaga catgcccgct ttcgttttgg
    33241 gctacctggt tttgagcatc ctcttcctca taccgctggt cggattcctg atcgagcttt
    33301 tatccatcag cctgggattc ggaggcctgg tcggctccct gaacgaaagt cggaggaggg
    33361 actgaaggat gctttagagg agatggggag acgaccgagg tggccgggag gcgatgtcga
    33421 gatgaatctg atttggcggc acggatcgag acccttgccg acgatagcag atgaggtcgt
    33481 ggtcgtatct ctggggggag tggagagagc cctccgtcca gaggatatcg ggatggtcga
    33541 gcttcgccga attttccatc aataatttaa tatagcaagc tatggattga atctatcgac
    33601 agccattcca cggttactat gaaaaaatcc atataccaat aagcatctag attcaagaac
    33661 gaagaagccc aggtgatgga cttgaagtcg attatagatg aggctcttaa ccgagctgag
    33721 ctcgagggcc gaacagctcc gccgggggcc atggagaacg acgacgatct cgaagatcgg
    33781 agagttcgga cgagggccaa ggagaacgac gacgatctgg cggcggttct ggagcagctc
    33841 acgacggtca ttcgggtagt cggctgcggc gggggcgggt ccaacaccat cgagcggctc
    33901 gccgagactg ggatcgaggg ggccgagctc ttcgccatga acaccgacgc ccagcacctt
    33961 cttcacataa gcgcggaccg gagagtcctc atcggccgga ggacgacccg ggggctgggg
    34021 gcggggagcc ttccctccat cggggaggag gcggcgaggg agagcatcga cgagatcagg
    34081 gccgcggtgg agggggcgga tatggtcttc atcacctgtg gcctcggcgg cgggacggga
    34141 accggagccg ctcccgtggt ggcggaggtg gcgagggaga tgggagccct caccatatcg
    34201 gtggtgaccc tccccttcgc cgcggagggg gcgataagga tggccaacgc cgacgtgggg
    34261 ctgaagcggc tccgcgatgt ctcagacacc gtcatcgtcg tccccaacga ccggctcctg
    34321 gaggtcgtcc ccgatctacc cctccaggcc gccttcaagg tggcagacga ggtattgatg
    34381 cgttcggtca aggggattac cgagctgatc accaggccag gcctgataaa tctggacttc
    34441 gccgacgtca ggacgatcat gtccaacggt ggggtcgcca tgatcggcct cggagaggcc
    34501 caaggggaga tgaagagcaa ggactcggtg ataaaggccc tcagaagccc cctcctggac
    34561 gtggatatct cgggggcgac gtcggccctg gtcaacgtag ttggcggccc caacatgacg
    34621 atctccgacg ccgagacggt ggtggaagag gtctacgaga ggatcaaccc cgaggcgagg
    34681 atcatctggg gggctcagat cgacccgaac ctcgaaaacg gcatcaggac gatgttggtg
    34741 gtcaccggtg tatcgtctcc tcagatcctg ggaagagagg acccaaagga ggggcggatc
    34801 gtctccaagc acgggataga cttcctccgg agaaggaggg ccgatatctg agcccaaatg
    34861 cccgggcacc tcgacgaaaa ggatcccagg gccaatgccg tcgcccgcgg aagcgaaccg
    34921 cctgggtcag cggcccgcca tgacgggggc tacaggatgg ggtgggtttg cgtcctgatt
    34981 cttgctcgcc tcccctcccc cggcggatga gaccttcggg acgtctccgt ccaccaccag
    35041 ctccaccttc tgcatataaa ggagggtctg gccgacacag gggaactctt tgaaggtgtc
    35101 agggtgaatg taaacggtct tgagcttatg acaggcctgg agcattctgt agatttcccg
    35161 gtttgtgggg acgaaggcga ctgccagctc ctcgtcggag ggtaaaagcc gcgccaggtc
    35221 gaactccgag ttcaagatcc gcatcatatt cagatcacct cgaagatctc catcagaaag
    35281 cccaagggtg gaagctccat atgccggcca actcttgctg attttacagt actagcaatg
    35341 gtcctatcgt atatgaatct gttgattaaa aataactggg gatagacccc tccctgaact
    35401 gaagggcatg ctacagcttg gcatgatgaa tcccccctgg cgcaggctgc ccgacctcca
    35461 tctcgtagac caaaaaataa gacgatatta ggtcggtacg gatcgcgacg cccggcgagg
    35521 ccgtcttcaa caaagatgac cccctggagg cttctcggat gggtaacccc ctcctcggag
    35581 cggacccatc ggagagcttt cgtcctggcc gcaaatgata acatgcacca tgtcaatcga
    35641 tggagttata aggtatagcg attgcttaat aaatattgtt atgatggatg acgctggatg
    35701 tgagaatatg gtgactgata aggtgaagcg agagcttgag atgctaaaaa ggcaccttat
    35761 aatcctgaaa catgtcatgg agaatgagcc gatcggcatc ctcaagctgg ccgaagagac
    35821 ggggatcccc agccacaagg tccgctactc cctccgggtc ttggagcagg aggggctcat
    35881 cgccgcctcc gccccggggg ccgttaccac ggagcggacg gaggccttcc tccaagatct
    35941 ggacatcgcc atcgacgggc tgcaggagat ggtccggggt ctcaaggaga taaagctcga
    36001 gcggtagctt ccgatgctcc cttacctaga gatcgcaagg tctctggagg cggtctccag
    36061 agaggaacgg cgaggggcca aggccgaaag gttggcggct cttatcaagc ccctccccgc
    36121 cgagatgctc cgccccaccg tcaggctcat ctcgggccgg ccctggcccc cctgggagcc
    36181 ccgggagctt gggatcggcc ccgagaccct ggatgcggtc ctcgaagaga tctccggccg
    36241 ggtcccctcc tctgaggaga gggcggctga ccccggggat cgggcggaga ggatggtccg
    36301 aggccggtct cagaggaccc tcgcgccgtc gaccatggac tccctgcacg tctatgaatc
    36361 tctccgccag atctcggccc agaggggacc tggctcctta tcccggagaa gagccatcct
    36421 caagggcctc cttctagccg catcccccct ggaggcgaag tacctggcca gggccgtcct
    36481 ggggaggacg acggcggggc tgggcccctc gctgatctcg gctgcgatag ggaaggcctt
    36541 cgatgtcgat tattccctgg tgaagagggc gtactcctac cttccggatc tggggatggt
    36601 ggccctcaac gcccgccagg gggacatata cgatctgagg ctcgcacctt ccaatcctgc
    36661 gaggatgatg tcgttccgac gggtgaagga cccccgggag ttgatggcgg ggggaggacc
    36721 gagggcctac gtcgtcaagt atgggggtct gagggtccag gtccacaagt tcaacgacca
    36781 ggtgtacatc tataccagcc agcttcgaaa tgtcacccgg ccccttcgcg atctggcgga
    36841 ggaggtggcg atggccggag gggagttcgt gatggagggg gagttgatcc tggtccgagg
    36901 gggaaggata tccccgaggt cggagacggt gggaaggatc aacctccggg ggaggtctcg
    36961 gggcggggcg atgccgtctc tggcggcgtc ggatctcctc tacctgaacg gcggggatct
    37021 cctccagaga ggatatgagg agaggagggg tctcctggcc aaggctctaa agggggtgga
    37081 aggaaggccg ttatcttcca gaatattcct ggcggaggag gaggtgattg gagatggtca
    37141 gagggcggag gagttcctcc gacggtctct ggatcggggg tttggtgggg catggatccg
    37201 cgatcttgac ggattgtaca tccccggcga gatgagctcc ggggacgcgg aggtatctcg
    37261 gttcccggcg gaaggaaact cctcctccca ggatcaggag ccccaccgtt aaattgccgc
    37321 cctcgccggc caaatgcggg gtgcggtcgt cgtccttccg gggcggtccc cgaccctccc
    37381 cgccgaagat ctccccaagt tcaaaataga tgtctgcgga agcgttttct gacgtaatac
    37441 ggccacgaaa gtctttatat gtgaagaata ctcagttcct tgagagccgg tcagcagggc
    37501 ggttcttgtc agaggggaga atatttaagc tcagtatgtg aataagttta gagaggtatc
    37561 ggaggaagga ttagatgaat agaggattct tggcggtttc ggcggcgctg ctggtggccg
    37621 ccatcacgtt atcgccagtg atggcatata ccatctgcag ctcagcaact ccatcttaca
    37681 ccataggatc gggaacgccc taccagtact cgataggatc aaagggcctc caggcctact
    37741 cgatcggttc cgggagcccg taccagtaca cgtcagggac tagcggcctc caggcctact
    37801 cgatcggttc cgggagcccg taccagtaca catcggggtc cagcggcctc caggcctact
    37861 cgatcagttc ggggagcccc taccagtaca gcgtagcatc gggagccctc caggcctact
    37921 ccatagggat gggagttcct gcggccagta ccggatcctg tccaatagtg gcgccgacgg
    37981 tggcgcccaa agtcgaggtt ccggttgtcg ctgatactga gaccgaggat gtcgaggttc
    38041 cggttgtcaa tgatactgag accgaggatg
//
LOCUS       DASM01000104           22442 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_105674, whole genome shotgun sequence.
ACCESSION   DASM01000104 DASM01000000
VERSION     DASM01000104.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 22442)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 22442)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..22442
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_105674"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    4362..4383
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4060 a         7196 c         6814 g         4348 t
ORIGIN      
        1 catcacctgg tgggtggatg tgggcatcgg gtacagtgcc aacatcgacc gggcgagaga
       61 gatcatgctc gaggtggcga ggaggcaccc cctggtattg aaggatcacg acataacggt
      121 gaggatcacc gatctcgccg atttcgctgt gaacatgagg ctcaccgtgg acgtgcctca
      181 cagggatgtg gcgtactcca ccgcctgcga cctccgagag gctataaaga agaggttcga
      241 tgaggagggg atcgagatac cttaccccta cagaaacctc atcctcctga ggggggaggg
      301 gccgtccccg gggcctgcag aagagggcct ggcgggggga gacctaccgg ccagcagatc
      361 gtcatgtcat gatgcgccct tcggaggagg ggccggacgg tgaagcccct ccggtcttcc
      421 gcctcatctc cccttagggg gcccccctgc ctatcgatcc tggagggagg attttatgat
      481 ctccttcagg ggtccgatct ctccggcctt ggcggagatg ggggcgatcc gatctcgcca
      541 ctgcctccag ggggggagca tccccagcct ttcgccgatg gcgtcgaggg tggcgtcgcg
      601 atccgcgacc ttgtccatct tgtttgcggc gacggcgacg gcgaggtcca ggtcggtcaa
      661 aaactcgaag agctccacct ccagggggat ctccctccgc ccctcccacc ggtcggcgat
      721 ctcgataaag ctggcggcgt cgatcacctg gatcgcgagg aggatctcgt cggccttctc
      781 ctcgaggtag cggacgatga agtccttcgt cccctcctgg acccccctct cggcgccctt
      841 catgtacccg tagccgggca tatcgacgta ggtgacggct ccagcccgga ccctcgcggg
      901 gcctaccgtc acccccggcc tcctccccac ccggacccgt ttgcccgtca ggaggcggaa
      961 tagggtggac tttccgacgt tcgaccttcc tatcagcact atctccgaag ccatggaccg
     1021 atcatccccc ttcctcctcg agcccccctt agaaattcgt ttctcttctt ctggggaagg
     1081 ggggttgttg gggaccgaaa ggcttatatt cgacaaatga cgtatgggaa ctaggcaaaa
     1141 gtcgattgaa ctttttcctc cctcccaaaa atccctacat ccgacttttg cccccccttc
     1201 attatcctcc gaccggtcta tgtcccgttg gccccttcca gcaggattgg tcttccgccg
     1261 gcgactgata tcaactctct cgatggggcg gcccccagga ggggtcggag gcgaggatct
     1321 cctccgagga agagaaaagt atatattcgt cagatgacgg agtaatgtca ggcaaaagtc
     1381 tgacctaaaa ccttccttcc ctcccgaatc ccaatgacat agacttttgc cccccacctc
     1441 ccttttatcc ttccctcctc ctagcgaagg cctgagggcg gcggatcaag ggcgccaaga
     1501 ccaataagct cgtcatccgc ctccggctca ccactggggc cttgaaattg ggcctaatct
     1561 ccgataaaaa aaatggcgcg gtgaagggcg cccctcggcg gatctccctt atacgctaga
     1621 cgcagccgaa gaccccggcc ccgctccagg ggctggtgtc gcagggatcg aggccggagc
     1681 acccttcgat gcagcaggag agccagtcgg tcttgttcgt cacgttcgta tccacgagct
     1741 ccatctttac cttgcgggtt atgtcgtagg tgccgctgta cctctcctcc ccgaggttga
     1801 ttaaatctcc ccggacgtct acctggcggt acctgaggtc tgcgatcccg gtggagtggg
     1861 atgcgaggtc gtagacggtg gacctggtgg ggaatacctc ggcgctgatg atggtatcgt
     1921 tggtggcgat gacggtggcg ttcagccgct caaggcgcag ggcaacgttc ctctccttcg
     1981 acagctcggt gttgtatagg aagctcgacc ccaccgagtc gccgttattt ccggcgcggt
     2041 cccggtcgtt gatcatcttc cccgcgtagt cgacggtctt gctggagttg agcctgaccg
     2101 gccagttctc ttcgagggtg atcgtccaga cttcgcttcc tgcgggcttg aagtaggtcc
     2161 ggttgacggc ggcctgggcc gatgcccgga ggtatatcgc ctcctcggag ctgtaggtcc
     2221 cctccagggc ggcggtcctc tccttcagcc taacttcgtt cacgtcgaag gtggaggagc
     2281 ccgagtagta aggcgagagg acccgaccgg cgggggtgac ggagatgggg gcgatgtcgc
     2341 cctccaccga gccggagagg tccagggtca tattcgcgct ctggcggctg atccagaagt
     2401 aggatccgtc ggtgacgttg tacaccccga aggtgtacga ggcggcggag gctgaggaga
     2461 tcagccccgc caggaggagc gcggtcgtta aagtgcgata tgcgtccatc ttcaaagctc
     2521 cacaatatct tgaccgtagt cgttcgcgtt agtccaggcc caagactcaa aagatccggc
     2581 cccagtccaa aacctttcct ctagtccgta agtcgattga gcccagggcc ataaaaagtc
     2641 cccctcggcg taggatatct gatgggccct ggcctcgtcg ccgtccacct cggcctcgcc
     2701 caaaaactcg aagtaagcgt tcccactgat ccagttgtcg acgtaggcaa ggtagtcctt
     2761 gtcggggttg taggcttggc agtaggcata ggcgtagtat ccctcggcca ccgccgactg
     2821 gcgacctgta gcgctgtttc cagctttgac cccctgggaa gcatccaggg ttccagcagt
     2881 tacggtggcg tagatgtcga cgtagctccc ctcgcagtcc ctcccttcac cgcgagccgc
     2941 agcgtaaacc ccttgggctt cgaccttctg agaagcagcg acgcttccgc cggtggctac
     3001 ggcctgggat gcggatatgt ccgccagaaa tccgtaagat tcaacgcctg ccacgtctcc
     3061 gccctggatg ctccatagct ccgcggaagt ctcgaaggct ccggccgtgg ctgcggatct
     3121 ggaaacgctt cccttcgacg gcatcaggca ggcgcttccc cggcccgaga tatctgatgc
     3181 tccaaaggtc tccagggcat aattaatgac gtagtcggca cctccgccgc tcccgtaaaa
     3241 ctcctggttt atatcggcgt tccccgagcc ccagaggcag ttctcctccg cgattcgaaa
     3301 ttcgccgtcg aagctcgccg aggccctgcc gctgaccccg atgctctcct ctacgttata
     3361 cgactccgcg atcccgacgg tcccgctgcc gctattcgcc gagaaatgga aatcgatggc
     3421 ataggccccc tggaatatca gaaggcaggt tatcgatatt gatatccgat gtaatccgga
     3481 cagagatctc cctcctagag ctaaactatt ggttgtcatt tgcacaagat aattctgacg
     3541 gtatcctgcc ggcaggtaaa agagtttcaa tcggcagacg gcatcctcag atagattatc
     3601 caaagtcgtg ttaggtatcc ctaaccttca agaatgtcgg agatcatcaa accaatataa
     3661 aaatggaaaa aatgggagag cgccctcgac cctcatccgc tagacgcatc cgaatacccc
     3721 ggccccgctc caggggctgg tgtcgcaggg atcgaggccg gaacaccctt cgatgcaaca
     3781 ggagagccag tcggtcttgt tcgtcacgtt cgtatcgacg agctccatct ttaccttgcg
     3841 ggttatgtcg taggtgccgc tgtacctctc ttccccgagg ttgattaaat cgccccggac
     3901 gtcgacctgg cggtacctga ggtccgcgat cccggtggag tgggaatcga ggtcgtagac
     3961 ggtggacctg gtgggtaata cctcggagct gacgatggta tcgttggtgg cgatgacggt
     4021 ggcgttcagc cggtcaaggc gcagggcgac gttcctctcc ttcgatagct cggtgttgta
     4081 gaggaagctc gaccccaccg agtcgccgtt atttccggcg cggtcccggt cgttgatcat
     4141 cttcccggcg tagtcgacgg tcttgctcgc gttcagcctg accggccagt tctcctcgag
     4201 ggtgatcgtc cagacgtcgc tcccggcggg cttgaagtag ctccggttga cggcagcctg
     4261 ggccgaggcc cggaggtata tctcctcctc ggagctgtag gtcccctcca gggcggccgt
     4321 cctctccttc agcctgactt cgttcacgtc gaaggtggag gnnnnnnnnn nnnnnnnnnn
     4381 nnnccgtcgg tgacgttgta caccccgaag gtgtaagagg cggcagaggc tgaggagatc
     4441 agccccgcca gcagcagtag atatacgaat accatcctca ttcagaccac ttccgcgacg
     4501 gcataagcgt tgctattgtc ggtccaagct tctcctgtga catcgtatgc tccaaagcct
     4561 cctttgggat cgatatcaaa atctgggggg tttgaggctt cagcgactat ctggaagtcg
     4621 ccaactactg tgtacttttg cttcgcccga gtggagtcgc cagcatacgc ctccatctct
     4681 ccaaagttca atccaccggc acccaatagg gtatacacct cggagtaagc tgtcccatcg
     4741 tcggcgtagg aatcaaggaa gatgttggtc cctaccgcgc ccacgttctc ggccgatgcc
     4801 gaagcgcttg agctcgtatc agcgctgaag tcgccccaga tcatggcgga cccggccgct
     4861 acatccccgg aaacgctggc gacccggtca ggtccgctgg aatcgtaggt cccgcttatg
     4921 gcaccgatga tcccatcgcc ctctatgacc gtgagccaca aattctctcc tgaggctgag
     4981 gtgctgtctc cggattctgc cataaggagg tcggcactgg cagcagccgt ggcgccgtat
     5041 cccccggcgc ccacagcgga accttcagcg acgagagaga tcggggtccc gcccaaaagc
     5101 tggagctcgg cgttcccggc ggcgacgccg gtacccgccg cacccgcggt gctccgggtg
     5161 tccacatcaa ttgcgaccac ttcggcgctg tttccgaccc tggcggcgac caatccgacg
     5221 ccgatctcgt tttcggcccc cgcggccccg gcaccgaaga tggcgccttc tgcctcgacg
     5281 atgccgttcc agctattgaa gtcgaatgct ccttccacgc cgccagcggc ggcggcgacg
     5341 acgacgtacc cctccttgtt ggaggcctcg atctctccgc caagggtgcc ggtcccgaag
     5401 aatccggtcc ccgcggcaag aattccgctg ctgatctcgt tctctttgcc gacggccccc
     5461 gcacctaccc ctgccgcctc ggcaccgcca gcgatgaaac cgttctggag gtcgacgatt
     5521 gcgcccaggc cgccagcggc ggcggctgcg gcaactccac cctccttgtt ggaagcttcg
     5581 atatcttggc cgatggcgcc ggttccatcc catccggttc ctgccgccag ggtaccgctg
     5641 ctgatctcgt tcattctgcc ttcagcgagg actccgaccc ctgcggcctc tgcgcttccg
     5701 gcgatgactc cattctggag gtcgaagctt gcgcccaggc cgccagcggc ggcggccgca
     5761 gcaactccgc cctccttgtt ggaagcttcg atatcttggc cgatggcgcc ggttccgtac
     5821 catccggttc ctgccgccag ggtaccgctg ctgatctcgt tccttctgcc ttcagcttgg
     5881 actccgaccc ctgcggcctc cgcccatcct gcgatgactc cattctgaag gtctaagcct
     5941 gcgcccaggc cgccagcggc ggcggccgca gcaactccac cctccttgtt ggaagctacg
     6001 atatcttggc cgatggcgcc ggttccgtac catccggttc cggccgccag ggtaccgctg
     6061 ctgatccggt ttctatatcc ctctgcgagg actcccacgc cagcagcctc cgcccatcct
     6121 gcgatgaaac cgttctggag gtcgaagctt gcgcccaggc caccagcggc cgctccagct
     6181 ccaaccgcac cctccctgtt ctgagcctct atgcctccgc cgtaggcgcc ggttccatcc
     6241 catccggttc ctgccgcgag aacgtcgctt gcgatccagt ttctccggcc ttctgccatg
     6301 acaccaacgc ccgcggcctc cgcccctgcg tcaatgaaac cgttctggaa gtctaagctt
     6361 gcgcccaggc ctccggcggc cgctgcagct ccaatcgcac cctccctgtt acgagcctct
     6421 atgcctccgc cataggcgct ggttccatac catccggttc cggccgcgag aacgtcgctt
     6481 gcgatccagt ttctccggcc ttccgccatg acacccacgc cagcagcctc cgcccctccg
     6541 ccgatgaaac cgttctggag gtcgacgatc ccgcccagcc ccccggcggc agcggctgcc
     6601 accacggacc ctactctatt ggaggcctcg atgcctccgc cgtaggcgcc ggtcccatag
     6661 aatccagttc ctgcggccag agttccgcta gtgatctcat tctttctgcc ttctgccagg
     6721 actccgacgc ccgcagcctc cgcaccacct tcgatgatgc cgttctggag gtcaacccca
     6781 actcccaggt ttccggctgc tgctgctgcg gcgagaatgc cttccctgtt ggaggcctca
     6841 atgcccctgc cggaggcgga ggttccggca tccccggtcc gggcttccag ggtacctttg
     6901 atctcgttat ccacgccgat ggcaccgatc ccggccgccg ccgcctctcc atagccgtct
     6961 atgaagagcc ccggaacgcc ccctgtccag tcggcttcga ggtcgatctt tcccgccccc
     7021 gcggctgcgc ctactgcccc gaggtcgctt tcagcatatc ttatgtcgcc ataagcccag
     7081 gcgctgtttc ccgtcaccgt ggcgagctta cagtcgacgg tcgcgggccc cgcgacgtcc
     7141 agggatgata ccgccacggc agctccctca ccctggaggt tcagagttcc agggcccatg
     7201 tttacgtcca tgtaacccgc ggtcccgaag gccgtcggaa ggtacccgac catctgagta
     7261 tcctgggagg tggcgatccc tccgttgacg ctgatcgtct gggtggattt gagggacccg
     7321 tccccgacga aggcgtgctg catcgccccc cggccgcacc ggtctgcggt ggagacgacg
     7381 gcgctcatct cagcgtcttt tatggagacg tcctggacga cccccagggc cccgggggtg
     7441 agatgggcgg accctcgggc cagggtctgg gatgcatctt ctgcgtagat gtagctcatg
     7501 ccgacgtagc cgccgctccc ggcatatccc tggacggcgt agatgctccc gggccctgcg
     7561 agccctctga tgtcgtcgat cttcaggcct tccccgaagc cggccgacga cttcccccac
     7621 accttgacgt cgtcgtcgac gttgtaggag tcccagatgc tcaccctatc tccactgcca
     7681 tcgctcgccg agaagtgcat atcgatggca taggccccct gaaatatcag aaggcaggtt
     7741 atagatatca aaagccgacg cgatacggac agagatctcc ctcctccagc tacaaccgct
     7801 tttgcatcgc acaagataaa cttttctaca agtcgtagaa atatcgtgaa ggagatatat
     7861 agaccttatc tgaacatact aaaaaataat tatttaaaat aaatagggat ttggggcgat
     7921 agcctctgcg gcatccgcgg cagaaccctc caagggttgt tgatgccgct atccggggag
     7981 gagaaacccg aggctcagag gcagttcttc tcggcgattc gaaattcgcc gtcgaagctc
     8041 gccgaggcgc tgctgctgac cccgatgctc tcgtcgacgt cgtaagattc gaccaccccg
     8101 ccggtcccgc tgccatcgct cgccgagaag tacatgtcga tggcataggc cccctgaaat
     8161 ataagaggga aagtcatgga tgggatcaaa taacgtaata tgtgcagaga gatctccctc
     8221 ctctatcata tgatatcttc ttattagaag aatataaata tttctctcct tcaagggccg
     8281 ctggaattag atatccgcca tagcttcgga gggcgtcgac cgatgattat caaaaaatat
     8341 atgtctcaca aggctggcgg aatcggattt acagttcaaa tgattctcgc ttctgccggc
     8401 ccggtgaacc agacggcgtt gtaggcctca tcctctgcca ggacgatcgg gacgtacacc
     8461 ccgtccatcg cctccacctc ttcgaggacg accctgtagt tgaagagggg agaccggctg
     8521 aggatcaaaa gctcgtgaaa ttccgtcgaa agggtctcaa agccgggttc ggcgatcacc
     8581 ttgtagaagg cgacggcggt atcgccccgg gaggcggtga cgttcttcgc cacgtacccg
     8641 acgtagtccg cccccggggc ggtggcgttg aggacctccc gggatgcgta gtggacggag
     8701 ccggactggt ctatcagggc gttgagcttt ccgtcctcgc cgaagtaggc gtaggcggga
     8761 acctgggtct cgccgaaatc gaggaggagg acgcaaggga gagatcgggc caggatcggc
     8821 tggagctctt ctctcacccc ctccaccacc gacggcagga cgggatagac ggttccgttg
     8881 gggtctgaga cctggaaggc cgtccccggc caggagaact ccgccgtcgc ctcccccccc
     8941 tccagctctc ggaagtcaga ccatactacc tcccccgatt cgtcatcggc gaggtagagg
     9001 gtcgccctcc ggaggttctc gtcggggtcg gagagctcca cctccatacc ggcggggctc
     9061 cccgggcttg cgccaccgga gaggaccttc aggctcacca cctccggggc gggggaggac
     9121 ccgagggccc ggatcatcca ttcggtctcg ttctgggggc tcccccccag ctcccaccgg
     9181 aggatgcggt tggggtttct cgccgtcaat agcgacttat tggtggcggg accgaggtcg
     9241 gtgcccacga agaccccgcc gaactcgtag agggcgacca ggaaggggcc ggagacctgg
     9301 acgtcaggga ggtccaccac agcccatccg aactcctccc cgaagtagga gtcggccctc
     9361 tccgtcacct tcgagacggc gttcaggtca ccgtcccaga tctccaggac gaagaggtcg
     9421 gaggtcctgt tcggctctag cttcccgtat accccgacag actttatcgt ccactctccg
     9481 gcggggggct cgaagacgac ggcgtggccg aggtcgccgc ccatccggaa gccgttctcc
     9541 gccgtcccgt cgtcgaggcg caaggcgacc agccccccgg aggtccccgc ccccgaggcg
     9601 atgaggaggg cgaggatccc ccaggttccc atcatcaaaa ccctccaact ggctgggcct
     9661 cgtaccccag cattgccacc aagacggcgg gcatcggcgg gcacacttcc gatcatagct
     9721 gggtattaga tccatcggcc tatttcacat ttttgaagcc tggggggcgc cacccaaaca
     9781 gataatacct ctcaggtcaa gtcgacctcg gagaggtccg ccttggagat gatcgccacc
     9841 acctgcgtct actgcggctg cggctgcggc ctcttcatcc acgtcgagga cggcagggcc
     9901 gtaggtgtct ccccaaaccc cgcccacccc atctccaggg ggaggctctg cgtcaagggc
     9961 tggctcgccg ccgacttcgt ccaccatcca gaccggctga gggcccccct gatccggcgc
    10021 ggcgggagga tggtccgatc ctcctgggag gaggccctca cggcgacggc cgagcggctg
    10081 aaggagatca gggacgaggc gggccccggg gccgtcgggg tcctcacctc ggcgaagggg
    10141 acgaacgagg agaactacct cttcgcaaag ctggcccggg ccggcctcag gacgaacaac
    10201 gtcgaccacg tcgcccggct ctgccacgcc ccctcggtgg cggggctggg ggccgccctg
    10261 gggagcgggg cgatgaccaa ccccataagg ggcctcctct cctccgacgc catcctcgtc
    10321 accggctcga acaccaccga gcagcacccc ctcgtcgccg ccaccatcct ggaggcccag
    10381 tcccggggag ccgccctcat cgtcgccgat ccgaggagga cccagatcgc cagcctcgcc
    10441 gaccaccgcc tcgcgccgag gctcggaacc gatgtcgcct ggatcaacgg ccttctgaac
    10501 ctgatcctcg aggggggtct cgccgatgag gagttcatca gggagaggac cgcaggattt
    10561 gacgccctga aggattccgt ctccaaatac acccccgagg tggtggagaa gatctcgggg
    10621 ataccccccc gggacctcga ggcggcggca gcggccttcg gcgaggcccc cagggcggcg
    10681 gtcgtctacg ccatggggac gacccagcac tccaacggga ccgagaacgt cctcgccctc
    10741 gcgaacctcg tcctcgccac cggaaacctc ggtagggagg ggacggggat ctgccccctc
    10801 cggggccacc agaacgtcca gggggcctgc gacatggggg cgctcccgaa cgtctactcc
    10861 ggctaccaga aggtggagga ccccgccgcc cgagggagga tggaggcggc ctgggggacg
    10921 gacctccctc cggcgccggg gctcaccgcc gtcgagatga tggaggcggc aaaggagggt
    10981 cggttgaagg ggctcttcat ctccggcgag aacccgatga tgagctaccc cgaccggggg
    11041 ctggtggggc gggccctcga atccctggag tttttcgccg tcgccgagat cttccccacc
    11101 gagacgacgg ccctcgccga cgtcgtcctc cccgccgcct ccttcgcgga gaaggacggg
    11161 accttcacct ccactgagcg gcgggtccag cgggtgaggc gcgcggtccc gcccccgggg
    11221 gaggcgaggc cggaggtgga ggtggcggca gccctcctga ggaggcttgg agtccccgca
    11281 gagtacgcct ccccggagga ggtgatggcc gagatagcct cggtgacccc ctcctacggc
    11341 gggatcagct acccaaggct cgggacggcg gggctttcct ggccctgccc cgatcccgac
    11401 catccgggga ccgagatcct ccacaaagag tcctttccta tcggccgggc ccggttcttc
    11461 ccggtggagc ttttggatat ggattcagac cccgagtacc ccttcctcct gacgaccggc
    11521 cggagcctct ttcacttcca caccgggagc atgacgagga ggacccacct cctcgaccgg
    11581 gaactcccga agccagccgt cgagatgaac cccgaggatg cggcggccct gggggtccgg
    11641 tcggggggga gggtggtggt ggagtcggcg gcaggcagcc tggaggtcga ggcccggacg
    11701 acggacacgg tcccccccgg gacgatcttt tttcccttcc acttcgccga ggctccggcg
    11761 aatcttctga cggccgcgga cctcgacccg agatccaaga taccggagtt caaacggacc
    11821 cccgtccgga tcaggaggct ggagggttga ccgcctccct cctccgccgc gaggggctcg
    11881 ttcccctcct ccgggatctg gcagagaccc tggaggtggt ggcgccgatc ctggtcgacg
    11941 gctcggcggt cttcgccacc tggagggggg aggagccggc cctaaaggcc gattaccccc
    12001 tcaactcccc agtggagctc ttcctcccga agagggaggt cctcttcagg tacgtccagt
    12061 acagcgggag gtacaccttc gccgaggagc cgccgaaggc gaggctgatc ttcgggatga
    12121 ggccctgcga cctccgggcc ctggaggtct tggaccggct cttcggcgcc gacatccctg
    12181 accaccccta cctcgccagg aggaggtcta cggtcgtggt cgtcttgaac tgcaccaccc
    12241 ccggcgagcg gtgcttctgc gcatccctcg ggtcggggcc cgcctccgtc ggcgggtacg
    12301 acctcctctt gacggatctg gggcgaggct acctggtaga ggcgggctcc cccgccggga
    12361 tcttcatcct ccaggctcat cggtgccact tcgaggagcc cctccccgag gaccacctgg
    12421 agaaggagag gctcctcgcc ggggcggagg aggagctccg ggggaggagg ccggccctca
    12481 gccctgaggt cctcaaaaga gcgatcgagg agacggactg ggaggtggcg ggtagcaggt
    12541 gtctatcctg cggcggatgc tccctcgtct gccccgtctg ccactgcttc accgtcatcg
    12601 accagggggt ccccgacggc gagaggctga ggtgccggga ctcctgcacc ctcgccggct
    12661 tctcgaggat gacgagcggc gcaaacccgc ggccgagcct cggcgagcgg ctccgccact
    12721 ggtacctgga caagttcgta tacatcccgg aaacgacggg gctcttcggc tgcgtcgggt
    12781 gcggcaggtg ctcaagggtc tgcctcagcg acgtcgaccg ctggtccctt ctgagggagg
    12841 ggccccgatg agggaggctg gccggagggc ggacggcaga gcccgccggg gaggagaggg
    12901 cgggtggggg gggaccgacg gctacgtccc cctccaggct gaggtcgtcg acatcgacgt
    12961 cgagtcgacg aacacctacc tcctctccct caggcccatc gacgatgagg acgagacggt
    13021 ggaggtcgac tactcccccg gccagtttct gatggtctcc gtcttcggcc tgggggagtg
    13081 tcccatatcc atcgcctcct cccccacccg ggagccgctg cagatatgcg tccgggaggc
    13141 ggggaggatc acccagggga tgatggccgc ccgggtcggg gacgtcctgg ggatccgggg
    13201 acccttcgga aacggcttcc ccgtcgcgga tatgaaggat agaaacgtcg ccatcgccgg
    13261 ggggggctcg ggctttgcca ccttgcggtc cctcatcaac tacatcgccg accaccgcga
    13321 cgactaccgc gaggtgaccg tcgtctacgg ggcgaggacc ccccaggacc tctacttcac
    13381 cagcgagtac gggagctggc aggccgccgg gatcgaggtg gggatcaccg tcgacatgat
    13441 ggacgcgagc tggaagggga acgtcggcct ggtgacgtcg ctcctatcag agatcgaccc
    13501 cgcccccggc gccgccgccc tctgcggtcc gccgatgatg atccgggccg tggcgaagga
    13561 cctcctgagc cggggattta tcgagcagga gatatacatc tccctggagc ggcacatgaa
    13621 gtgcgggatc ggaaagtgcg agcactgcac catgggaccc taccacgtct gcaccgacgg
    13681 gccgatcttc agctacgatc gggtgaggga tctgatctga tgagccgtca tctctccttc
    13741 cggttcctgt agatctcccg gagggcctcc cggtgcatcg aggcgacggc gtcgccgagg
    13801 atccccatga agaagagctg gaagccgacg atgatcagaa gggcggtcag gatcgtcagc
    13861 ggaatgtgct ctgtccgggt ggtgatccat tccctgagga tgtagagtcc gaggatgaat
    13921 cccgccgtgc cgaagaagga acccatcagg ccgaagtaga agagggggtt gtgggttttg
    13981 gccattcgat agatggtggc gacgatcctc gccccgtcca tgagggggtt gagcttcgtc
    14041 ttcgtcccgg tcgcccgagg ccggtaggtt atggggacct cggctatggc aaggcctttc
    14101 ttgacgctgt ctacggtcat ctccgtctcg atctcaaagc cctccatggt caggtcgagc
    14161 ctccgaaccc cttcccgagt gaaggcccgg tatcccgtca ggatgtcgct cagggggacg
    14221 tcgtagatca gccggaagaa gaagtttatc agccggtttc ccgccgagtt gaggcgggtg
    14281 agggctccag gctccaggtt tccgaaccgg tttcccatga cgtggtcggc ctttcccgcc
    14341 aggaccggtt cgaggaggag gcggacctcg ccagggaggt aggtcccgtc cccgtcgatc
    14401 atgacgatct tctcggcctc gatgaggctg aaggcctcct tcagggcctg gcccttgccc
    14461 ctccccctct ggacgatgac ccgggcgccc gcctcctcgg cccgctctcg ggtccgatcc
    14521 tggctgtggc cgtcgatgac gaggatgtcg ccgaagccca tcctcttgaa ctcgccgatc
    14581 accccggcga tggcgtcctc ctcgttgagg gtcggtatca ggatgcagac gtcgttttgg
    14641 gtcaactcaa atcagctctt tctctcgccg ggtggtcggg ggactggggc gtgggtcgac
    14701 ggcaggatcg tcggcctctc caaccgtcct tcacctcgtg gccccttcta tgaaccccat
    14761 aaaccgcctc accagctcct ccacatccgt ccccgccgtc tcgacgtaga gcctgatgag
    14821 gggctgggtg ttggagggcc tgatgaggac ggtgtaatcc tcggtggtga ttttgaggcc
    14881 gtcggtggtg tcgggctcaa gaggcgcgag gtcaccctcg agccgcctca tgagatcggc
    14941 cgccgaaccc tctatcctga cggacctctg gacgtagggg tagtcgggga tggcgtccgc
    15001 gagctccgag agccgctcgc cccgggagac gatctcgccg agggcgaggg acatggcgaa
    15061 ggggtcgtcg gagagctgga actccgggat gaagaagtgg gcggaccgct cgaccccgag
    15121 gactgccccc tcctccttga cccggtttgc gacgaagacg tcccccaccc tctcccggat
    15181 cacctcgaat ccgaggggta gaagctcccg ctccacgatc atggagcagt cgaagcccgc
    15241 gaccaccttg tccccggggg cgtacctctc cttcgccagg atcaccgcca ccttctcggg
    15301 ggggacgatc cttcctcggt cgtcgatgac gaggccccgg tcggcgtcgg ggtcgaagcc
    15361 gacgccgtaa tcggctcccg tctccaccac catcctcgcc gcgtcctgga gggactcctc
    15421 ggtgggggcc ggcccccgac cggggaagag gccgtcgggg tcgccgttcg tcacctgaca
    15481 gtcgaagccc agcctctggt agagggggaa ggtggcgcag gcggcgccgt ttcccatgtc
    15541 caggacgatc cggaggggcc ggccgaaggt gagctttcct tcgacgtagt cggcgtacct
    15601 ccggagggcg tcgtcggggg atcggtccgt cagcttgccg tcccccttcc ggaaggtgcc
    15661 cttctcgtag aggttcatca tctccgtctc gtggtagagc atcccgtacc cctcgctggt
    15721 cctgaacctg atcccgttgt actcgggggg gttgtgggag gcggagatgt acgccgcggc
    15781 gttgtagccc ccccgccagg tctcaaagct gatcatcgat atcgggagga cgccgtacct
    15841 ctcgacggag catccggtgg ataggacgcc atccaggaag gccctctcca ccgcggggcc
    15901 gctcaccctg gtgtccctcc cgagggcgat cctgccgtcg gcgaggtagg atccgagggc
    15961 ctttcccacc cggttcgcga tctccccggt cagctccgtc ccaaagacgc ctcgcacgtc
    16021 ataagctctg aagactgtca cgttcaagag gtggagagga tatctaaaaa tagttttcta
    16081 aagcggcccc tcttttatcc gccgcatatc gatcataacg ccgcctctct tccctcgatc
    16141 gtcccgacgg cgtggccgga cctgatgggg ccgatccgga cccgcagccg gtcgccgacg
    16201 cccgagccgg ggacgaagat ccggaacccc tccacctcgc caaacccgtc cccggatttt
    16261 ccgacgccga cgatcaaaag ctccaaaacg tccccggcct tcaccggcgg gataaagaac
    16321 ccctggccga cgacgtacat ctcggcgctc tccttccggg aggcggccgg agagtgggac
    16381 tgggcccgcc tgaaccgccc tatcacctcc gaaaggtaat ccaaaaaggt gtccccctgg
    16441 aagaccttca ccaggaagtt tccgccgggc ttgaggacct cctctgccac ccggagggcg
    16501 gaccgggaga ggtcgatgga ccgcgcgtgg tcgagggtcc agttccccga gaggttcggg
    16561 gcggcgtcgc agatgacgac gtcggccttc ccgccgaggg cctcgacgat cagctccagg
    16621 gtcttttggg cggtgatgtc ggccttgatc gtcgttacac catcgatggg atcgatcgcg
    16681 aggaggtcga ccccgacgac tctccccccg gagatctcct ttgccacctg cagccacccg
    16741 ccgggggcgg cccccaggtc caggacggcg tcgcccctcc ggatcagccg gaacctctgg
    16801 ttgatctgct ggagcttgaa ggccgaccgg gcccggtacc cctcggcctt ggccttccgg
    16861 tagaagtagt ccttttgatc cctggccatc aggggatatt ttcctcccag ggcgataaag
    16921 ggcttgtggt tcgacccaga tggaaaatca ctagattcac gtgagaagga taaggcaagg
    16981 aagggtgtat ttgagttcac ctggggagag ccatctcctc cctccctcgc cttctttcct
    17041 gatgtagtcc ttttctgcct cagcctcgat agacctcttt gcctcctaca taagtcgcta
    17101 gaacccttgg attatccatg gacatttcat cgaagggtac ggaatagagg tccttttcat
    17161 acacgaccat atcggcctta tatccgggat atagaacgcc ggtatcgttc cttgatgtgg
    17221 tggcggtgct gccgacagtg aaaccgcgaa tgacttgcgc aacactgagt ggcaggtgcg
    17281 attgcggatc tcctccgccg gtaaccgcga cacggatcac catagtggca ggagagtatt
    17341 ctagaggtga aacgctgaaa tctgtgctga tcccaacagg ggttccgctg tctatgagct
    17401 ttttaatggg gtatgcatct ttattgtgtt ctccgtaata ataagcggtg tcattctcga
    17461 cggaccagaa ggcaggctgg accgtcatga cgacatgtcc gtcgaatttg cggatcttct
    17521 cgatctgctc atcattcggg aatcccatgt gtatcagcgt gtggactcct gttagatttc
    17581 ctctggctga cgcaacctta tcaagagcct caattactgc ccctatggcc ctatctccct
    17641 ggacatggta gtgcatattc ataccgtaat cttcaaccct ctcgactatg cgtgtgagat
    17701 tcatctctgg tccaccggca tcatcagtat atatctcctg gnncagccta caaaccggac
    17761 catatccgtg tcgttgtcgc ggtatctcgc agcatcgtca acgtcgctca ggttaaatat
    17821 agtatagcaa taattgatcc gcagcggaag ggtgccttct tcttccatct cataaaatag
    17881 atctggcctc atcagccgca cgccgggacc acccatcagg tctaccgctc ctgtatatcc
    17941 gtaggatgcc cagcgtttca gcaaaataag cgtaccggcc ttgatatccg tgctgttgaa
    18001 caaagcgaat atcttatttc ccggcaatgt cattgcagat tcccgcagca ttccagtaag
    18061 cctgccatcc tccaccccca tctttccgcc gagaggcaca ggggttgtgg gagtaaggcc
    18121 ggtcaggttc atagcggcac tatttataat ggcgttatga ccgagcttgt cgcctaaaaa
    18181 ggctggcctt gagcccgtcg aattatctat cttggcaagg tcttcaagcg accagctatc
    18241 gtagtcccgc agcgagaaac cagcaccgag aatgattccg tcttctggaa tatatttggc
    18301 tttatcagcc agaatccgtg caatagaatc tgaatcgctg cagcctatca ggtttgctcc
    18361 gacatagaag aagtaacctg tttccataag atgcacatgg ctatcgataa aaccaggata
    18421 gatggcagcg ccctttagat cgataactgt tgaattttta tattcatcgg ggctgacgtt
    18481 cagtgccttt accacgccat ctacgacgaa cagattatca gcttttttag tttcatcaac
    18541 ataaattgat ccattggtgt agagctgagc gattccttct ccggtggcac ttccaataaa
    18601 agccaaaacg caaagcactg ccaatataat gccgaaatca gtgatctcca ttctcagcat
    18661 agccatcctc cttgtaattg tcttactgtt aatcagatat ataagaattc ggatgatttt
    18721 tagatttaga gtcatgaaac tatttctcac ctcatctagg cccagaatcg tcctcaggga
    18781 tatgaaccga gcaagacgaa gattcgttgg ataattcgtc agaaacgcaa tagtatggct
    18841 acaaaagata tcgtccgcaa catgaaggta tccgcgagac ttctgcagca gattctcaag
    18901 gaatatcgcg agacgaaggt ggtaggttgc atcggattgg cccaaaaaga gccaccatat
    18961 tggatcgggt tgcttgttag gttttttatc tgcaacaact cgtttatagg tagaccacca
    19021 atttcgtccc catcagaaac cttcatcacc ccctttcccc cacaaaacaa ccgtgaactg
    19081 cgacgttctg gtggtggggg cctccccctc ggggatcgcc gccgccacag cggccgccga
    19141 gggcggcgcc gaggtgatcc tcctcgacaa agacatgggc gaaagcttca gccacccggc
    19201 caacaccttc ttcgacggga tgttctcccg ggcgggcctc gcggtcgagc gggagtacgt
    19261 cctccacgac ctcgaaggga tgtggatcat ctcccccggc ggctgcgccg tcgagatccc
    19321 ggcgccgggg aggttcatcg atcgatcaag gttcgacgcc ctgtacctcg aacgagccga
    19381 gggggccggg gccgagctta tgcggggcga ggcgaagagc gcatcccatg cgggcgccgg
    19441 gcggcgggtc gagaccgact ccggcaagat cgcggcgaag gtcgtcatcg acgcctccgg
    19501 ggtcgatgga gccattgcga gaagagaggg cctttcgccc caacgccatc cggaggacgt
    19561 cgcctgggcc gctgaggccg ttgttgaatt agcgggcctg ggggaggaga ggcgcttcga
    19621 gtactttctc ggctcgatct cgccggggtg gaaggcgacc ttctcccccg ggggcggcga
    19681 tttggcgacc ctgggggtct tcgtccgggg gcacggccgg gacgtccgcc ccttcctgga
    19741 ccggttcgtc gagtttttca agaaatacaa gtccgccgat tacgacgccc gcgagatgaa
    19801 ggtcgtcgcc gtcaaccagg gcggggacgc cattgcgacc ctgcccggcg agatcgtcgc
    19861 cgactccctg atggcgacgg gcgccgccgc cggcatgagc gggatcgcct acgccatccg
    19921 ggcggggacg atcgctgggg atgtggcggc ccgggccgcc gcgtcggggg acgtctcgaa
    19981 gaagggcctc tcgggatacg tccggctctg gcgccgggag ttcggcctgg agtaccggat
    20041 gggccgggcg tccctcttga ccctcggcag aatgcccgac gaggtgatag accggctggc
    20101 cgcgggcctc gcgaagaggg acctcgacct ctcaggctcg ttctccagga agggcctctc
    20161 ggcggggatc gccctggcca gggcgcggcc caggacgatc cccagcctgg tacggagctt
    20221 cgtcgagggc tgatcagcga gtgagggtcc aagacggaat caactctatt agttaggcca
    20281 cctcgacgtt gctgctctcc tccctcgccc cgccgtcctc ggggagcttg atctcgccat
    20341 aggtcgcctc gtacctcttc aggttcgcgg atagggcccg cagcagcctc ttggcgtgct
    20401 gaggggttat ggtcacgatc gccctcccct gggccaaatt cgtctcgggg atcttgtgga
    20461 ggaaggtgag ggtgacctcg tcgtcggcgt ggctgatctt gatcaggttc gcgtagaccg
    20521 gctccagcct ctcggggatc ctgaccccca gctgctcgat ccttttttta tccgtcaatt
    20581 aacttcaccg cgggtccgtt ctcttgaggt ctgccttatt cctttgcctg cggtagagga
    20641 aaaatccttt aaccgcccag gcggtctcct ccaccatgat agagctgagg agtgaccctt
    20701 acgtcctgaa gtccaggacc gtccaggata cggagctgga cttcaggata aacctgaacc
    20761 tctcctggtg tcgcggcgtc ctcttcaacg tggtggcgat gaagaactcc gtcgccatcg
    20821 tcgagtacga cgccatcctc tggaccgatg aagctctcat gttcgtcgag tacaaggact
    20881 ccgtcgcggc ttacaagaac ctctccaccc ggaggatcca gcagatcgga agcttcgcca
    20941 agaacatcgc cgcgggccta ggctaccggg cctacacctt cctggtggtg gtgaacggtc
    21001 tgacggagga gaccgccagg ggaggggcgg tcgtcatacc cctctttctg cttggaagct
    21061 acgagccctc cttcagctcc gcggtctcgg agctggagct gatcgataag ctgatcgaga
    21121 agtaccaccg gtctgggagc cccgaggtgg ccgaggactt cgagaagctg aaggcgatcc
    21181 tcgaggcggg gttgccttga ggcggagggg aacggcgacg gccctcggcg ccgccacagt
    21241 cctcaacgcc gtagccaact ggaaggggtc ggccttcgga gtcgacctga agacctccgc
    21301 cgaggtggtc ctggacgaga gcccggcggt caagggggac gttcccggga tcgacgaccg
    21361 tctgatcgtc aggtccgtcg aaagggtcct ggagaggttc ggcgtcgtcg ccgggggcgt
    21421 cgtcaggact tggagcgata tccccctcgc ccgggggctg aagagcagca gcgccgctgc
    21481 caacgccgcg gtcctcgcga ccctcgacgc cctcggcgag gacctcgacc ccctggaggc
    21541 gacgaggatc ggggtcgcgg cggcgaggga cgccggcgtc accatcaccg gcgccttcga
    21601 cgacgcctcc gcctcgatgc tggggggggt cgtcgtcacc gacaaccgaa ggctcgagct
    21661 cctcaagagg gaggagatgg agtccgaggt cctcctcctc gtcccggagg agaagatctt
    21721 ctcgaaggag acggacgtcg ggaggtcgag gcttctcgcc ccggtggcgg acctgatctt
    21781 cgacctcgcc ctggcggggg actatcccag ggcgatgacc ctcaacggcc tcgtctactg
    21841 cagcgccctc cgcctcccct ccgacccgat attcgctgcc ctcgcctccg gcgcgagatc
    21901 tgccagccta tcggggacgg gccccgccta cgccgccctc gtcgaggacg aaaatatcga
    21961 cgatgtcgag gcggcctggc tccatctggg cggaagggtg ataaggacgg aggttaacaa
    22021 tcagggctca atcagaggat cggggtggcg aggatgagcc tggaagatct ccgcgcagaa
    22081 gtccaaaaag ttgaccggga tataatagcg ctgatctacg agcgggtgag gctggcggag
    22141 gatatcttcg aggccaagcg agccttgggc gtcggaatcg aggacaaaaa ccaggagaag
    22201 ctggtgatga agcgcgccct cgatatggcg acggagctcg gcttggacgc cggcgccgtg
    22261 aaggagatct tcgcgatcct gatcaggatg agcctggaga agcagcacga gcttctgggg
    22321 gagaacaatc tcccttagcc agaggctcga agccaccggg ttcggagaga ggaggtttca
    22381 gttggcatga ccgagatcgc cgtcatatcg gggtccaaat cggaccggcc cgtggtggag
    22441 aa
//
LOCUS       DASM01000105           10174 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_119814, whole genome shotgun sequence.
ACCESSION   DASM01000105 DASM01000000
VERSION     DASM01000105.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10174)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10174)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10174
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_119814"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1790 a         3322 c         3175 g         1887 t
ORIGIN      
        1 ctctggttcg ggtggtttgg gttcaacgcc ggatccgccc tcgccgccga cggcctggcc
       61 gcgagcgcct tcgtggtgac gaacgtctcc gcctcggcgg gggccctggc ctggctcttc
      121 gcctcctggg tgaggggaaa gccgtccgcc ctcggaatgg tgagcggcgc catagctggc
      181 ctcgtagcca taaccccggc ggcggggttc gtcgatcccc tatcagccct ggtcatcgga
      241 ggagtcgccg gggtcctctg ctacagagcc ctcctcttca gggtggggag gggcctcgat
      301 gagagcctcg acgcctgggc ggtccacggc gtcggcggcc tctggggggc cgtagctacg
      361 gggatcttcg ccgtcgaggc cgtcggcggc gcctccggcc tcctccaggg gaacgtcgac
      421 caattcgtcg cccaggtgat cggggccgtc gccgccgtcg tctacgcctt cgtcgtcacc
      481 tacgccctgg cgcgggccgt cgacgcgacg atgggcttgc gggtcaccga ggacgaggag
      541 tacgtcggcc tcgacatatc ccagcacgga gagaaggcct atgcctagcg gagggatagg
      601 ctggaagaaa cccgagatcc gatcggatgg aggtgatcta gcgatgatga aggtggaggc
      661 cgttatccgg cccgagaggc tggaccaggt gaagaaggcc ctggaggaga ggggctttgt
      721 ggggatgacc gtctccgagg tgacggggcg gggcgagcag aaggggatca agctccagtt
      781 cagggggagg acgatggacg tcgacctcct ccccaaggtc aagatagagc tgatcgtaaa
      841 agacgaggcg gtcgacgatc tgatcgcgac gatctccgcc gccgcccgga ccgggcggcc
      901 cggggacggg aggatcttcg tcatcccggt ggcgaggtcc gtccgggtga ggacggggga
      961 ggaggtgatc tgaaggccaa ccttgccacc tccctccatc ctcctttcct ttttggttcc
     1021 atctcttgag cagatccaat ctctttcctc ctccgcctgg accaagaatc tctgtggggg
     1081 atcggccgtc tatccgccta tcggggtcgc tgccagatcc ccatcctctt ctggacggcg
     1141 gtcacctccg gctcgggggg gacggggggg ttcttgaggg agaccgaggg gtcgccgtag
     1201 aggttcatga cgtaccagtt catcgtcgtc tcgctgtagg tcccggggta gacggtccct
     1261 ttcgcctggt atagggcgtc gccggcggca agctcatcga cgagcctgtc cacgtactcg
     1321 tagccgagcc ccgagtttga ggggctacca tcgaagttgc catataactg atttacgtag
     1381 taccaggaga ccctcgtcgc cgagatcgtc ccgatcccgc cgttcttcag gatggagtag
     1441 gcgaggttat cggtcgtctc aggctcggcg ttgagacagg agcactgata ggtgaaggat
     1501 gggtgggcgt cgtcgaggct ggcgatgtag gagctagcga agaggaggcc gccactgcat
     1561 ccggaatagc ctatggacgc cgaagtcggg ctcccgtggg cccaccagca gactatcccg
     1621 tagtcgccgg cggaccaccg gtctctgacc accgtccctc cgtggagctc ctcctcgcta
     1681 ttatacttgg agtctgcaca cgacccgacc gtcccctgct ggtacatccg ccaggatgag
     1741 tagcccccgg ggttgagata atcgtccctc atctgctcgc tgaggcgggc cccgtcggtt
     1801 acggagtcgc tgaaggccat ggggaggagg gtggacctcc gccagtcggt gccggtggag
     1861 agctcgtagc ggatggtctt ctcgaggatg ctgtcgaggg cgggataatc ggcgtcatat
     1921 acggggatcc tgccgacgta gacctcgggg gtgaagtcga cgcctccggc gacgggatag
     1981 tccttcccca agccccactg gccgtagatc ccgtcgccgt tcttatccca gttgccggtg
     2041 aggtcggcat agaagtagtc ggtgggcgcc tcacgatgga tagtctcggg atacctcggc
     2101 cagcacatct tcatggggat ctccccctcg ccgctctcga agggccgggg atcgcctatc
     2161 aggaggacgt actttatccc cagggaggcg tagttgttct tcagccactg gcggatcttc
     2221 tccgccctgt tgttcggcgc ctggcccgtc aggggcagaa agtcgctctc agtgaccacc
     2281 aggacccggt atccgatcct ctccttgtgg gcgatgaagt cggcgagctt tccgctcccc
     2341 gcgacgatgg cactggtggt gatgatgacg taatcgtaag ccccttccgc ccgcgaatcg
     2401 agccggtcat cctcggcccc gaggtaccag agcctccctt cttcgtagtt gtcgaagagc
     2461 tggggggcaa cctcgtccat gaccgcgtcc gccgcgaagg cctcatccaa gggctcgggg
     2521 gaggaccgat agctgatctc cacgacgcag ctcttgatca gggtgagctt ctttgagacg
     2581 gggttgtact ggagggggaa gaagtcgacc cgggcaaact tccacttcct catctcggag
     2641 taggggagga ggacggcgaa ggtctccggg tagttcgcat ccctctcgta gacggcgagg
     2701 ttcctcccgt cggatatcgt ctttcccagg ccccagtact cggcccaggc caccccccca
     2761 aattcgtcgc cggcgagggc aggggcgagg ggtcccaccg gcccgatctc gtacgtcccc
     2821 tccaggatct cgacgtcctg ggagacgacc ttcatcgtga gctcctcccc gatcgcctcg
     2881 ggggggacga ggatgttgta gacccggtga aggaggaggg ggtcccccgg ggaggtggtg
     2941 gaggcgaagc cctccatcgc caccacgtcg aaccccgccc cgtcttcggc gatggagagc
     3001 acccccgcct ccaggaccat ccggaccgtc cctcccggca gatccgaatc cgatggcggt
     3061 tctggtcctg ccggagacgg ccccccgtcc tccggggccg ccggggccga taggccgatg
     3121 aggcttagta ggaatatgat cccgatgacg ccgccgatgg cgagacggac cccggtggcc
     3181 gcccttcggg gcgtccgccc gccgtccctg gaaccctgaa ctcctttaga agcacctggc
     3241 gtgatgagta cccccttcta cttttgcccg attacgctta tgtatagctt gccgatcagc
     3301 tccagtcctg tcatcgatgc ccaaaaaatc aggccgaggg agtcccatcc agcttcggtc
     3361 tcatcggggc cgaaacgatc gatctctcct tatatccagg ggcttcggat aaaaagactt
     3421 tccttgcagc cgtctccgag gctccgggcg accaaagccg ggccgatcgg agatgtggga
     3481 gaggagggag ctccggtggc tgaggagcga ggtcgacgga gccaccctat tgattcgaca
     3541 tttctagcga agaataaata taaatatatt attctagtat actcgtaccc tcatccaccc
     3601 tcccccagaa actccaccac cgccaccact gtctcctccg gatggctgat ccagaagagg
     3661 tgccccgccc cctcaatgat gcgaagctcc gcgccgggga tcttctctgc cagaatcaaa
     3721 ccgttctcag gcgggatcag gatgtcggag tcgccggcga tgaccagggt gggggcccgg
     3781 atctccgcca gccggccgcc gatatcgaag gcccggtagg cggccagctg ggcccggtag
     3841 gcctcgaggc tgctcgggtg ggccatattc gcctggacct ttcgctcgac gatttccggg
     3901 tgggcatcga tgaactcttg gggataccca gcgggggcgg accaccagag cgcctcgtgg
     3961 agcgagaggt cgagccgcgg ctccaggtat gcctgaacct ccgggggcgc gctgaccgag
     4021 gctgcgcccc cggggctggt ggcccccagg atcaaacggc cgatccggtc cgaatggcgg
     4081 gcggccatct cctgggcgat catcccgccc atggagacgc cgaagacgtc cgtccgctcg
     4141 atcccgaggg cgtccaggag gcctacggcg tcgtcggcca tctcggcgat ggaatagggg
     4201 ccggggggct gctcgctgcg gcctgcgccc cggttgtcga aggcgatcac cgagcggccc
     4261 tcggccagct tttctggcag gacccacccc cagtcgagga tgtggccgcc gaggcccatg
     4321 atcagaagga ggggctcacc ctcgccgtgg atctcgtaat acaatctgat ctcacccacc
     4381 gtgatgtttc ctccggctga agagggctca tccccgtcca tcatcccggc ggccgccgga
     4441 ggggccgata gaagaagaag aagaagaaga agaagggaaa ggagggcgaa ggtggcggcg
     4501 gcgacgccgg ctccggcccc ggccttgggc ggtgaaggag gcctgcgagt ggagctcatg
     4561 cccggaatca ccctccaaaa ggttgtaaat ccctttcccc gccggtccca ggtcagcgga
     4621 tctcctccag gaggtcgtgg aggttgaagt gctcgtaccc gccccgctgg gccggaaagt
     4681 cccggatcgc cagccacaga tccccgctcg cggggcgggc gacgagggac cacatgttgg
     4741 cgatgtgggc cttgaaggcc cccacaggct ctctagcccg gaaggcgaag tcggggtaga
     4801 gggcggagat ggtgcagagg atgttgttgg tccaggaggg aaactcctcc ggcctggtca
     4861 tccgggtgta ggggtcgtag cggtcgctga ggatctcgac ggccacctcg ggggtgatct
     4921 tcccgtagtg ctcgtggatc agctggctgt acctctcaaa cctgctcggc gcctggacgg
     4981 agaagttgta ggggtctcgg agactggtct gccacctctc gacggtggat acgtcgagaa
     5041 gatcgttgac gggggcctga tctctcacca tgttgtaccc cgagtagccc atccagcccg
     5101 gatagcagtt ggagtggttc gtctgccaga gagcctcgac cccctccatc gggcggcgga
     5161 cagccaccct ctttgaagag gtctcgacga ccgccgcctc cctcgccccg gcgtcggcga
     5221 cgtggtaggc gatccccgca caccggggat ggtcccggaa gattcgaacg gcgtcctcga
     5281 cgctcctcgc ggaggtaaga agaaggcggg tgaagacggc gatgccgcat cccgccatcg
     5341 actcatgccg catcgaggaa agctgggtca tcatctcaaa ccccgcctcg ttgacgcccc
     5401 cgtcgaggcc gatcatcccc ggataggtca tgaaggcgtg gccgaagccg tcgtctgggg
     5461 cggcgaccac catcatccga cggtcgagct ggcctggcat gttgaagttg tcctcgttct
     5521 tcccgaagat cagctcgccg tcccctcctg ccccgtcgcc ccaggcggag aagctgctgc
     5581 acccccctgc aggaggcgcc gggggcgcag gccccaccct ctctccctca tcggcaacat
     5641 cgccttcgca gttccaatag tgaggatggc agtagatgca ccactggtcg tagttggtgt
     5701 tccataggag gatctcctcg aaagtcgcct tacccccggc atcccggacg ccgtcagcga
     5761 tccccgccat ctcctcctca agctccttcg gaagatgcgg ctcggccctc ctctggccga
     5821 tccagagcat ctcctccgcc cgctccagct ccgagccggt ctggagggcg acgtaggcgg
     5881 cggtggccgc tagcgtcgta tcgaccatga gatcgatatc ctcggccagc tgccgtccgt
     5941 gctggtagcc catctcgtag gggctcccgg cgaggcggag aatcttcacc cccctcacct
     6001 ctttcagcac cgccctctcc agcacctcat ctctcatatc cgccccgtcc cccgatgaag
     6061 atctctcctc cccggctcgg ccccggcatc aaaccggggc cttttatcgg tcccctcagc
     6121 tcgcgccatc gtcgaaggtg gcatcagcgg aatggaaata cctgacgacg accgattcgg
     6181 cttcgggatc caccgatatc acctggaact tgacgtagcc gtcatggcac ctggcgacga
     6241 tggtatgccc ttccaggaga gggggtatca ggggagatct gtcccattcc gcagggacgg
     6301 ttcgcaccgc ctccagatcg accgacccca tgtccctggt ctggctgacc cggttctggt
     6361 cgtcgaggtg ggtgttcctc caccagaggt agccggcgtc gcgaggataa tctggatggt
     6421 cttcagcgct gggctgccag cttatgatct ctccatcgaa ggtgggatat tcgccggttt
     6481 cacccaacga gaagtcgaac cccgaatggg tgagggtcgc ctcccgatac gaagtcgccg
     6541 tcccattggc agtaccatct gataccacct tcacccctcc ggccttggtc ccgacggctc
     6601 ccccctcctc agggccggaa ccgctcgccc caaggcctcc tccgacggcc cggaaggagt
     6661 agcccgtgtt gtgggacttg cccgccaggt cgacgatccc gatgtagtac cagccgggtc
     6721 ccggagcctc atatttgagg gtggccaggt cgccggggtt tgaggcgtct gctctcgtga
     6781 tgaagttgaa gttcttgccg tagaggtcga tcctcgccct catctcatcg gggacggatt
     6841 cgagcatcac ctccaggtcg ccggggctgt cggcccatat cttgtagaag tctacgtctc
     6901 ccgccgggaa gatgacccct ccgacagcct cgccggggct gatgggggcg gcctccccta
     6961 tctcgctctt cggctcgtta tcgtccacca ccggctcgaa ggaggcggcg aagctgtaag
     7021 ggaccttaag ggatctgccc gccaggtcga cgaccccgat gtggtaaact cccgggctct
     7081 tcacatcaaa cctgagggtc gtcttatccc cggggtttga ggcgtcaatc ctggtgatga
     7141 agttgaagct cttgccgtag aggtcgatcc tcgtcctcat ctcctcgggg acggatgcca
     7201 tcttaacctc caggatcccg gggctcggag cctcgaactt gaagaagtct acgtcccccc
     7261 tggagaagat gtacccctct atctgatcgc cgaaggcgat ggccaccgca tccccgatct
     7321 cgccgttcgg ctcgttctcg tcgatcaccg gctcaaggtc cgccttgaag gtgtagggga
     7381 cgtggtagga gcctcccgag aggtcagtga tcccgacgta gtaacttacc ggataccagc
     7441 tataaggatt gaccacgtcg tgcttcaggg tcaccagatc tccggggttc gatgcatcga
     7501 ccctggtgat gaagttgaag ttcttgccgt agaggtcgat cctcgtcttc atcccctcgg
     7561 ggacggactc ggccttcacc tccaggatcc cggagccggt tacatcgagc ttgtagatat
     7621 ccgcgtcccc ctttgggaag atgtatcccg agatgagatc gccaaagccg atggatggtg
     7681 cgtcccctgc ctcgccgttc ggctcgtgta cgtctacgac gggcccaaag gaaagatcga
     7741 aggagtagcc ggtatcgtgg gacttgcccg ccaggtcgac gatcccgatg tagtaacggc
     7801 ccgggtttgc aagatcgagg gtgagggcca ccaggtctcc ggggtttgag gcgtctttcc
     7861 tggtgatgaa gttgaagttc ttgccgtaga ggtctatcct cgctctcatc tcctggggga
     7921 gggcgttcaa cttcacctcc aggatccctg ggctttcgac ctcaaagccg aagaagtcca
     7981 cgtcccccgc cgggtttatg gtccccgcgg cggaaacccc tgggctgatc catgtggcgc
     8041 ttccaagctc gctattcggc tcgttgggat ccatctgaga ataccccaaa gtcgccagca
     8101 tcaaaagaac aaaagttgca tagatcagcc ccggggctga ccgccggaaa atgcccttca
     8161 tgccggatat atctctccgg gatctgccaa aaactttgcg gatgggggcg gcggcctgga
     8221 gggaaaaacc ggagcctacc cccccggccc cagggtcacc aggacgttgt ctagtccgcc
     8281 gacgtccctc atgttgctgt actccccccg gatccagacc tccgaaacct cctccatcac
     8341 cccctcgaag cttttaccca ccaccctgaa gctcgaaggg acgagggata cctgccggcg
     8401 ggtccagctc ttcccggggg ggtcgcacga ccaggagagg taggacccgt ctttgccgta
     8461 aatccggacc acatccccaa aggtggagct gccgttcccc gagtcgatct cgacgatctc
     8521 gaagctgaga gtacccccga tgtagccggt ccagtccccc gcccaggagg ggggagagac
     8581 gtagtaccag gtctggcccg acccctggtc ctccccccgg agatagcctc ccggatcccc
     8641 tcccgacccc cgccaggtga gggcgacgtc tcggcctccc cgccacccct cgtccccgag
     8701 gtcgaaggtg ctgaggaggt cggttgccac cggcggagag acgacgacgt tgtcgatccc
     8761 ctcctcgtcc cccctatccg agtactcgcc ccggatgacg acccggtcca ccgacctcat
     8821 caccctccag aaggcctcct ccgtcgtccc gaaggcggcg ggggtgaggt cgacccggag
     8881 gcgggtccat cccgactcgg ggttcggccc ggagggggag gaggggacgg agaccaccga
     8941 cccgtcggcc ccgacgatcc tcaccagatc cgcctcgtaa ggcccaccct tccccgagtc
     9001 gatcaccttc aggtcgaagc tcaacgaccc gccgacgtaa ggggtccagt cccccgacca
     9061 ggaccggggt gaggcgaagt accaggtgct gccgtctccg aggtcccggc ccgagaggaa
     9121 gcctccgccg tttccgcccc gctcaaccca ggcgatggag acgtccccct cgggcctcca
     9181 ccccccgtcg ccgtcgtcga agggcgtggc caggtccagc cccgaggggg gggcgacgac
     9241 gacgtcgtcg agccccaggg tcgtccccgc ctcaaacccc aggggcccca gggtgatccc
     9301 cgccaccgac ctcataaccc tccggaggtc agcctccgag accccgaagt tggaggcgtt
     9361 taagacgacc tcttcccggg tccagaacct ctcggaggga ggaggagaag aggagaggac
     9421 cgtcccgtcg gaggagagga tcttgacctc cacccccggg ggctcggagc cgccggaggc
     9481 gatgagcctc agatcgtagc tgaatacccc tccgatgtac cggctccagt cgccagccca
     9541 ggatgggggc gaccgatagc tccagatgcc gtcgccctct ccctccagaa agccgcccgg
     9601 atcgcctccg aaccgctgcc atctcacgtg gccgcctccc tccgagatcc atccctcatc
     9661 cccctcgccg aagccgctct ccagggtggt ggccgacccc tccagcgcca ggaggacgag
     9721 gagtgccatc cacatcccgc atatcgccct catctcagaa tcgcctccct ccagattatg
     9781 cataagtagt ataatatctt ttgtctagtc catccaaaat tcgatgcagt tatcctggat
     9841 aaggttggca tcagcccgcg tattctttgg ataaaggcgt aagttctgat ggaaatatcc
     9901 tgataaaacg gagaagacgc ctcgaagatc aagggagggg ccctcatcaa aaatacgtaa
     9961 ctgcaggtca tgtttcgcaa caactaaata ttgatcccgc aaaccacctc tgagatgttc
    10021 gaggcctggg acgagtacca tctgatcaac ggggagcttt tggggctgac ggagacgctt
    10081 cctgcctcgg ggctggggga ggtgatcagg tacgtcctca ccacccccgg gaagcgggtc
    10141 aggcccctca tcctcgtcct ctcctcggag gcct
//
LOCUS       DASM01000106           14856 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_95671, whole genome shotgun sequence.
ACCESSION   DASM01000106 DASM01000000
VERSION     DASM01000106.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 14856)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14856
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_95671"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         3081 a         4373 c         4415 g         2987 t
ORIGIN      
        1 ctcggaggcg gacctctcgg aggcgaagct ctggggggcg aagatatcgg gggcgagcct
       61 cgtcgacgcc accctcacca aagccgatct gacgaggacg gacatcaccg acgccgacct
      121 caccggggcg gagatgacga acgcccggct cttccgggca gacctcaccg gagcgacgat
      181 gaccggagtt tacctgatcg gcggcaactt cgtcggagcc cacatgaact gggcggagat
      241 gagaggaagc tacctcaacc gcggccagtt ctccagggcc gagttctacg ggaccgacct
      301 ctccggctcc gacctctccg actccgactt ctcgagggca tacctgatgc gggcaaacct
      361 cagcgaggcg aacctcaact gggccctctt cgcctacgcc gacctgacgg aggcgaagct
      421 atctcgttct accctccggg gaactaagct atcctacgcg gacttgaccg gcgccgacct
      481 ctcgggggcc gacctgaccg acgccgacct caccgccatc cggctgataa agtcgaacct
      541 cagcaacacc aagatgggcc gggcttacct ccaggggctc gacctccggg gggtggacct
      601 atcgggggct tatctgaggg atgccaccat cgacaggacc tacctcaccg acgccaacct
      661 gaccggggcg gacctccggg gcgccaccct ctcgtcggtg gagatgacgg gggcggacct
      721 cgcgggggcg aacctcatcc gggcgaaggt cgaccagttc accctcgtat ccctctcgaa
      781 ggcgaatctg gaggggaccc tcttggacga agagctgaag agggatatag atgcaataaa
      841 gtgagggggg gcccgcctag gggcccttcc cccatccgat cctaccggcc gaggatgacg
      901 tggtagtcga cgaagggcct gtggtccagg gtgatcagac ccctatggcc gcggatctcg
      961 tagggctccg cgcagaccat cgtcccgatc atctcacggc actcgaagtc gctgtagtag
     1021 ttgccggccc accggttgcc gccgttgtcc cgcccgttga acttattgtt gtcgatcagg
     1081 gcgttggaca tgacgaggtt caacccccca tcgaagaggt atatccctcc aagccccgag
     1141 ttctggatca ggttggagag gaagaggttc tgggcgctct gatccagaac ctggatcccg
     1201 tattcgccgc tccccaggat gtcgttgtct gagacgatcg tccccgtcga tctgatgatg
     1261 tggaccccgt cggcggcgct attcgggatc ctgttggacc agatgttgct gttgtcgcat
     1321 ctgaagaggc ggatcccgtc cctcgtgctg ttgacgatct cgttcttcga gacgacgacg
     1381 ttatcgacca cctccagact caagccgaag ccgctgtcta ttatcctgtt ctctacgatg
     1441 gagttgtcgg aagagtaccg gatgatgagg gcggtcgagt ggttgtatat ctcgttcgcc
     1501 tggacgaagt ttgagtcgct cttgtcgaat acgatgccgt atctcttgtt ctccgataag
     1561 ttgttgtaga ttaccatgtt cctcctgcta tccagccgga gggatatgcc gctctcccga
     1621 ttgctccgga cgacgttatg ggagatggtg ttgtagctcg ccccgaggat cgagatgccg
     1681 tcgaggtcgt tattcaatac ggagttccag gccatgacgt tgttgtcgcc cgaatcgacg
     1741 ttgatcccca tcccgtcgca gccctcgatg gcgtttgagg cgatcatgat gctgaaggac
     1801 cggtagatgt cgatcccgct attcacgcag tcccggatcc gatttccgac caagatgttg
     1861 ttgttggaat ccaccaggta caccccgttg tcggcgttgc tgtggatcga gttggaggtg
     1921 agctcgctgc cggtggagga ggctatggtg atgccatcgg cgcggttccc ccagatatcg
     1981 ctgtttgaga cccagttgtc atgggatcgc tcgatgaaga ctccgtaccc gttattgctt
     2041 atattgttct ccgcgatatg gctaccgttc accccggcga accggatccc cgccttgaag
     2101 ttgtcgcaga tcctgttctt tgagatcagg tgacccgtgg agtttagtac gcagatgccg
     2161 tggctcattg agccgtatat ggtgttgttc atgatcctgg tgccatccga gaggacgagg
     2221 accgaccccg aatcgcagga gtcgttggtg catccgccga tgatgaaacc gtccaggacg
     2281 acgccgctcg cagttatagt tatgatgggg gaggtcgcat tgcacccctc gacgaggggc
     2341 atcccctctc cccggagggt aatgggccga tcgacgacga ggttctcccc gtaagtgccg
     2401 ttcaggacga ctatctcgtc gccgatctcg gaggcgtcta tggcgtcctg gatcttccag
     2461 aagtccttgg tgccagagct gtcgacctgg agggctccgg ctccggggcc gaccaggatc
     2521 aggatcagca ataccgctgt tggccatctg tacatgagaa tcactcaccc ttatggtcag
     2581 tcgctcttta tcagtctgct catttcagtt caaatatgag attatatttc tagaatttcc
     2641 ggcgatgggg gattgggggg cttctgcccc cccggctcat ctccccaggg cgacgttggg
     2701 gtcgctgaag gccctccggt cgagggtgac cgcccccctc tggccgcgga cctcgtaggg
     2761 ctcagtgcat agaacctgcc agaatgtctc ctcgcactga tgatcgctgt agtagttacc
     2821 gtcccagagg ttgccgccat tatcccagcc gttgaacttc gtgttatcga tcagggcgtt
     2881 gaaggaggcg aggttgaact ccccctcgaa gaggtagatt ccgcccaggg ccgagtcctg
     2941 gatcaggttt gccaagagga ggttctgggt gctctgatcc aggatctgga cgccgtactc
     3001 gccgctcctc aggatgtcgt tgtccgatac gatcgtcgcc gtcgatcgga tgatgtggat
     3061 cccgtcggcg gcgctgtcca ggatcctgtt cgcccatatc ttgctgtggt cgcatctatt
     3121 gaggctgatg ccgtctcttg agctgttcgc gatctcgttc ttcgagacga ccacgttatc
     3181 gaccatctcc agactcaagc cgaagccgtt atcccggacg acgttctcga cgacggagtt
     3241 gccggcggag tatctcatga cgatgccgtt ggtgttgtgg tggatctggt tctcatggac
     3301 ggagttcagg tcgctctcgt ccaagatcag gccgtacctc ttgttctccg atatggtgtt
     3361 gttcgctatc atgctgtttc tgctatcccg gcggagggag atgccgttgt cccggttctt
     3421 ctcgacgacg ttgttggaga tggtgctgtg gtcagatccg aggatagaaa ccccgttgat
     3481 gtcgttggct gataggaaat tccggttgac gacgatgttg tccgtctcgt cgaggttgat
     3541 ccccatctcg ccgcagctct cgatggtgtt gaagacgatc agagtgctga aggacctcac
     3601 cacgtcgatc ccgctatttc tgcagtcctg gatggtgttt gccacgatta tgttgttctt
     3661 cgaatcgagg tggtataccc cgttctcaga gttgttgtgg atctcgtttc cggtgatctg
     3721 ctggcctgaa gaggcgacga tggcgattcc atcggcaccg ttggcactga tatcgttata
     3781 tgcgatcaca gccccctcgg atgcctcgat gtagattccg tatccgttcc cgcggacctc
     3841 gttatcggag atctggctcg agttcgcccc ggcgagctgg atcccggccc tgaagttgtc
     3901 gtggatccgg ttcttagaga tcaggtgacc cgatgagttg aggagagtga tgccgtggct
     3961 cattgagccg tatatggtgt tgttcaggat cttggagccg tccgagagga cgaggaccga
     4021 ggctgaatcg caggagtcgt tggtgcatcc ggcgatgata aaaccgtcca ggacgacgcc
     4081 gctcgcattt atagttatga tgggggaggt cgcattgcac ccctcgacga ggggcatccc
     4141 ctctccccgc agggtgatgg gccagtcgac gacgaggctc tccgcgtaag tgccgttttt
     4201 gacgactatc tcgtcgcccg cctcggaggc gtctatggcg tcctggatct tcgagaagtc
     4261 tttggtgcca gagccgtcga cctggagggc tccagctccg aagtcgacga ggatcaggat
     4321 aaggattatc ggagtttgcc atcggatcat gataatcact cacccttatg gtcagtaact
     4381 ctttattaat ttactcatat tcctggtgac ccaagaaacc ggcatctgat ggatttgagg
     4441 gcgtgagctg ccccggccca ccaccaccgg accgcttcat aggaggaggt tttctgccgg
     4501 gccccttcat aatgctctgc cgccattttt gggctttggg aggggagacg gcggggagga
     4561 gtcgatccgc accggaagga tgtgatgccc acgaagggag gaaaaaaagt catcctccgg
     4621 ctcggaggat ggtcgcctcg ccgcctctct ggagccacca ttatgaccag gtgccttcag
     4681 cttcggtggc attggcctct ttatcctcga tgacggtgat cgtggcattg atcccgtctt
     4741 cctcctcgcc gcattcggga cactcctccg gggcggcctc ttctggcagc tctgatgcgt
     4801 caaggacccc agccttggtc atctccacga ggtggatggt ggtgacgaag gtggcattct
     4861 gggagatggc gtaatccatg aacctattga tggcatccag atactctcca gagccggaga
     4921 cggtgttggt caggctcacc accatcggct cgtcttttcc ttctatctca tcgaacttgc
     4981 cgacgagggc gtcgtaccac tcctctccat ccattccggc atcggagaag cttccgtcat
     5041 ggaggacgac cctatcgtcc gataggtcgt agctgctcac cggaacggcg cggaagttgt
     5101 gcccctccac cggatagggc cagacatcgt tctcatggcc gggctcgaag agaagccctg
     5161 cctggaagcc ggcactgtac tcgatctcca gcaggtccag ggcagcgtag gtatcctgat
     5221 tctgatcgaa agactggggc atgaagcctt tagcgtttat gacgttggtc ccgcagacga
     5281 cggtggactc gacgtacctc ttggaggtct ctaacaaccg gctctgctcc agataagaga
     5341 ggtcgctgag cttctcatcg gagtgcttgc cggagatggc gaactcgaac tccgactcaa
     5401 ggcccatgcg ggtgagccgg attcggcccc tagtgtctat gaaatcttgg gttgagaaga
     5461 ccgtggcgac gaggtctctg ctggatatat tggtgtaaag atttacaagt cccagctccc
     5521 cagctttctt ctcctccgcc gtcgcctccg gcgggacgcc ggccgtgatc atcaggttga
     5581 ctatcgtcgg caggtctgcc gcttgcgccg cgccgaagat tgcgatgatg gatacaaggg
     5641 ctattaatgt ggatgccaat cggactccca tagttcattc acctcatccg tcgatgactg
     5701 caaccgtatc ttaacctcat catacgatac gatactacaa aaactcttcg agggatgcct
     5761 atataattca tcgaagcgat atccttattt aggaaaggag cgcctcttaa aaagtcagtg
     5821 ggcaatattg cccggagatg agaggtgcgg gatagaagcc ggagggatgg cttctaccat
     5881 ccgattctgg agggataaaa gatgaagaag acgcgtctta ggatagagat ctgggccctg
     5941 gcgttagcga gcgtcattgc cctggcggcg ccctctgcag gtgtggcggt ccccagctgt
     6001 acgcccagca gctgggcgaa tgtatttgtt aaccaacacg tggagctcca agcggatccc
     6061 gatgacagta ccacctactt ttacgattgg tcagcgtctt caggagtgac gtttctcgat
     6121 cctcccgggg tagtagataa caccaaatca gcgattacgt cgcccgccac ggctggagat
     6181 tacacggtgg acgtgatcat aacaaaccag atggcacccg catgttcggc tcagaactgt
     6241 tttggtctga gggtgtacga atgctgccca gatgctgaaa cggactattg taccagcaat
     6301 aaggatacat ggtgctggta cgactcctgc ggcaccgcag ctcccttcac cccgtccaga
     6361 tcaatcgggt ttgagtggta catcaacgat gccaaggagc ccatcagcaa ggagccatgt
     6421 tactcccccg acttcctcaa tgatggatac gatctgccat caaaagaggc ccccaccacg
     6481 ccccagaagg tgacgatgaa ggtcgtccag gatctgaccg gttcggagta cggggcaacg
     6541 catcactctg atccgtatgt gctttacgag tgtggcctcg agttcaacct ccactgggat
     6601 ccggagggcg aggtgaaggt atcggtggca ttcagctgag ggctccgccg aagctgcgtg
     6661 accttcggcc gccagcttca acatttttta tcatcggata gagaagactt agacctagat
     6721 tttgccgaag gaaaccgtag agtacgatag atagatgaga ccagagatgt ggaggcaggc
     6781 ggcgtgaaga gaagatcggt tgcgtacatc ttcctaatcc tcgggatctc tcaggccctg
     6841 ctgtcggcgg ggcagtcgag ctgggacccg tggtgcaact atcccggagg ctcctgtact
     6901 gccaacgata tttccgtcac cagggtctac ctcgtccaga actccccgag ttcagtgacc
     6961 ctcatgggga ccttcagcct gaatctggcc accgatagat attgcccctt ctccgtggtg
     7021 gacatatacc gaggggacgg ggtgacgccc atagaattga agcacgtcac ctggcttacc
     7081 accctctcgg gagggacgtc cccccaggag atcccccttg caacgataac tcgccccccc
     7141 gatgagccga tagtgttaaa aaatatatac gtccagtggt cagcgagcaa gtccacatgc
     7201 ggcgataact gtaacaatta tcagccctct aaatgctata aggacccagg tcctctggtc
     7261 gtcccagctt cgctcgtcgc caacttttcg ggcttggatg tttgccttgg aggatcgatc
     7321 cagttcgccg acctgaccac cgggggctat aagccctacg cctcgtcggt ctggaccttc
     7381 ggcgacgcca ccacctacag ctgctcttca gcctctccct gcaacccccc tcccaaaact
     7441 tacgcctctc caggaagata tacggtcaac ctgagggtga cggacaataa aggcgtagtc
     7501 tcgacgaaat cccagggcta ctacgtctgg gcccccccgg ttgcagactt cagctactcc
     7561 caggggtgcg ggttcgaagt tcagttcacg gacctctcga cggcaacgcc aacggccgac
     7621 gtcatgccca cgatcacggc gcagagctgg gatttcgacg gcgacggcgc tatagatagc
     7681 tcaatgcaga gccccgagta caccttcccg gccccgggga cctatcccgt tcggctcacc
     7741 gtaacggact cgcatggatg caccggcttc atagtcaagg atgtcaccgt aaatccagcc
     7801 gttcaagcga cggccatatc caactctccc gtctgtgagg gatcgacgat caacttgtat
     7861 gggggccccg acggcctgat cagctactcc tggaccggcc ctgatgagtt cgagagcagc
     7921 ctgcagaacc ccaccatcga tcctgccacc ctggccaacg gtgggactta tacgctgaag
     7981 gtcgtcgatg aagaaggctg cgagaccgaa gcgaagacta cggttctggt tcagaggagg
     8041 ccggcggtct cggcaggggc ggacctctac atatgcgaga cggaagagca gatcctgata
     8101 gatggatcga attcgggggg tcctgccaca tacctctgga cgacttcggg cacaggccac
     8161 tttgagccag agggggggct cagcacccgc taccaaccca gcgatgggga cagggaggcg
     8221 gggagggtta atatcaccct gacggccgtc gggattggcc cctgcggcac cttcagcaca
     8281 agcgataata tgaccctatc gatcttgaca gtccctgagg catcgatcaa tgtggccttc
     8341 tgaggatatg ggccgaggag gaccgatgga gatggaaacg aactcccttc ttatgggaaa
     8401 tgatcggata actcgccgtt ctgggaggag aggggcgata tctcctttca ccttcacatt
     8461 gcttctaatt atatccctcc tccttccagg ggtctacgca gaggttccgg ccgcttatta
     8521 tgctggcgag acctatacgt tcaccaccga cttagatcct catcttttca tcttcgactg
     8581 ggaggcattc tgctgcagca atcctgcctg ctgtgacgat ccgagctgct gtgggacaaa
     8641 aagctgcggc agccatgagg gggcctctgg ccaccagttc gtatggacgg cccccgatgt
     8701 gaactgcccc accaacgtca ccgtctcggt cctcgccgga gtcaagacct accccgcatg
     8761 taatggcctc gctctgatca acctaaccat ccagcctcag acctgcagca tcaccgtcgt
     8821 caaggagacg gaaccggtgg gggacccgga ggccttcaac ctccgcctct catacggccc
     8881 gaccgagttc accctagcag ggggtgaggc cgagacgttc agaggcctcc ctccgggaag
     8941 ctacgtcatt ggagaggagg atgtgccggg ctgggaccta tccggcatcg gggtggaggg
     9001 ggcgacttcg gcccggtaca gctccgacgc caccatctgg cacgacgact tctcctcggg
     9061 ggacggtttc ataaaagtta ccatcggccc tggagagacg gcgacggtga acttcaccaa
     9121 cattaagctc ggatccatcg tggtcgggaa ggtcacagag ccgaaagggt ctggcgaggt
     9181 cttcgagttt gctgcaagct acgccgacca aaagatcctc ctaaaggatg gcgagacgaa
     9241 cgcatcggga cttctggtgc ctggaaccta caccattgaa gagatcatcc ccgagggctg
     9301 gaccctcgcg gatataaacg gagccgcctc catcgaggga tcaaaagcca ccgtcgacct
     9361 cggccctgga gagacggcga cggtgaactt cataaacaag aggggaggct ccgtcgaggt
     9421 caccaaggtc gtaaactgga gcagctatcc ctttgaccat agggcgctcg ttgataccgg
     9481 ttttgagatc tgcatcggga acgagtcctg gaagagctgc aaaaccgtgg gctccgatgg
     9541 cggggtcctc gtctggaacg acctcgctcc gggagtctac aacgtctccg agaaggagaa
     9601 cggcctcatc tggacggtca ccgtcgatgg gtctcccttc atcatagatg ggctgaccga
     9661 tgcggcgaag caggtaaaga tcaccaacga atacaccgat cctgagcagc tcaaccccgg
     9721 gttggaggtc accaagacgg cgtcaccgac cgcgggggtt ccgcatacgg agatcacctt
     9781 caccatcgtg gtgagaaaca ccgggaaagt ccccctggcc ctcgaggtga acgacaccct
     9841 ccccgcgggg atgagctaca tctccgcctc cccagccccc gacagggtcg tcgagaacgc
     9901 cgacgggacg acggagatct tctgggatgg cctcggaact ctgggacccg ggaacgagac
     9961 gacgatcctg gtcgtcgccg agatcgggga ggatatacca aagtccgccc cttcggcgga
    10021 ggggctgacg gtccttggat ccaccgacct cggcacgggt gctgatccgg ccggagaaga
    10081 ggtcctctgg gtccagcagg taggccgggg aggcctcgcc gataccatcg aggggctgat
    10141 ctggctgagg gtgaggctgg aggtggagat ggcgaagatg atcgagctat cgaggaggtt
    10201 cgatccagga tcggcaggcg tcgtcttcga cgagcgggag ggggaccttc cgggagttct
    10261 cgtcaaaaac tacacccacc ccgccaccga tgagcggctc gtcctcttcg tcgacgccgc
    10321 gggaaatatc accagatcgg agtactacaa cccggagatg gactcggtcc tcacctcgga
    10381 gtacggggac gacggcgacc tcgtcgccga gagcctccta tcggtctcga tgttggagag
    10441 gctgaggata gactacgata ctccggcccc cgggtacagg accagaacgg tgacggacta
    10501 caagaccgga gacaccctca tcgagagggt agacccgacg ggggaggtga tccagagaga
    10561 ctaccggagg accccgggaa tccccaagga gaagcagttc cggctccgaa acgccgtaac
    10621 cgctgccggc agatctgacc ggggtgatgt caccgactcc gactacgccg acgtgaccgt
    10681 cgcctacctc tcggagctga ggctgacgaa agtggcatct ccaataccgg cagccgtcgg
    10741 gaccgtcatc agctattcct acgccgtcga gaacgtcggc ggcaccacca tagcgaacat
    10801 caccctcttc gacgaccgcc tcgaccggct catcgacctc aacaggacga ccctgaagcc
    10861 cggcgaattc gccggcggga ccgccgatta caccgtcgtc accgccgacc tgccgggccc
    10921 catcgagaac aaagcgacgg tgaccggcac cgacccccag ggggacgaga tcacggatga
    10981 ggataccgcc accgtacccc tctacgcctt cgctgcggag atcgacctca tcaagacccc
    11041 cgacaggacc aacgtgacgg tgggggaggt ggtgacctac tccttcgtcg tccagaacgt
    11101 cgggatcgcc cccgtcgaag gcctcctcct gtacgacgat cggctcgacc gcttcatcga
    11161 gctgaacagg acagccctga agcccggcga gatcgccgtc gggaccgccg attacaccat
    11221 cctccctgaa gacattcctc gggtcgtcaa caacgcgacg gtgacggggg tggcggtaag
    11281 cgagatggac gatagcgagc agcccgtctc cgccgaggac tccgccgtgg tcaatgtaac
    11341 cggggcggat aataaaacga agccaccatc agacctcatc gtcaccaaaa ccgccctcca
    11401 gaaggccgtg aggccggggg acgaggtgac gtacaacatc accgtctgcg gcccggcggg
    11461 gacgcgggtg aatgttcgag acgtattcga ccggccggtg gagttcgtat gtgccgagcc
    11521 ccggccgttg aagatcggag atcggtggca ggagtggaat ttgaccctac ctggtggaaa
    11581 caacaccacc tgcgccacca tcatcctcca catcaagacc ctgaggaagg agttcacctt
    11641 cgagatggag cagggggtct ccggggaggg gttcgtcaag gtcgcgaacg actacaatac
    11701 ggaaccacca tcgtacaccc tcaccaacgt cgtcacggcc acgaatgtga gcaacaccaa
    11761 cgaaacggac gacgatgcag aggcgatttt cgtctccgag gccgggacgg agctatcgac
    11821 tcgggagcac ggctccggga gctacgacag cgatgaggtc ctccggatga ggaccgagaa
    11881 taagtccatc tcgatggaga aggaggtctc cgccgcctac gcccccacca ccctcggact
    11941 ctacaacggg agggtcctcg agtactcttc ccgatggagc caggaggcga ggggcaaaaa
    12001 ccgggccact ggagcctcga tggccgaatc ctaccggtac gccacctcca tcgaccggga
    12061 gtctaggatc ttcctcgaca ggaacgagtc ggtgatggag atcgacgccg agttcgatgg
    12121 ggtggcccag ttcggcttct tcaagatgcc gtccaactcc tccggcccga gatcgaaacc
    12181 caccttcgag atggaagaga gttatgcagg aagcttcaag gtcctccaga gggtcgacga
    12241 gtacggccgg ggcgtctcct acgagaagtc agcttcgggc tcgggcctcg tcgtcggaga
    12301 tagaagagta gggtcatctc agaggtccta cgaggccggg gccggatcct acgaatccga
    12361 ggagatcatc gagaccggca ccaactacat cgccaaggat atcaacgtca ccagcgcccc
    12421 tgcgtccctg aacctcaccg actccacgaa tatcaacgcc tccggaaggt ggaaggaggg
    12481 gatctactcc agggcggagg gggtcagcta catcggagag gagtactact ccatcgaccg
    12541 gctcgaacgg gaggcgacct tccagggcct gaacgatcta tcatcggagg ccgatttctc
    12601 cggggctgcc cggtacaggg tcgtcgtggc caacaggtcc aaaggggccg gctctgaggg
    12661 ggagggggac ctgcccctca tcgattatga cgaggtatac tccggggatt actccgtggc
    12721 gaggaagatc gtcctctccg gcgtccccaa gtacgaccac cctcacctat ccatatccaa
    12781 gatcagcaag gccgataaga actccagcca ggtcaggtac gccatcacaa taaccaacga
    12841 cgggaacgcg cccctccagg atataaccgt cgaggacgta ttcccaccga ggacctcgta
    12901 catcagctcg tctctcaggc cggagatagc cgccgatagg gcggtatgga gcgtttcgcc
    12961 ccttccggcc ggagggagat tcgagatcga gctcgtcctc gaagtaatag aggacggcgg
    13021 atccctggta aacgtcgtca aggcgacggc cagcgccggg aacgataccc acctcaccgc
    13081 cagcaacttc tccgtcctgg aggcggggtg gctatcctgc tgccccgccg agatcttcgc
    13141 gagcaagacc gggtccgtcg accccgtcct cgagaacatc ataacctaca ggctgacggt
    13201 ccagaacctc cagaacgaga cggtggtggc caacatcgtc gacaccctcc cctccgggtt
    13261 atccctcctc ggggcttcag tcatgccggc agagtacgac cggtcccggg gtaggataag
    13321 ctgggtcatc gcggacctga acccccggga ggtgaggacg atcgagtacc tagtcgaggc
    13381 ccggacccgg ggaaggttcc tgaacgtcgc ggaggtcgag gcgtacaccc tcggagggac
    13441 ggagcttggg atcgtatcca tctccgccgt cgtcgatgtc ggagacttcg aggaggctga
    13501 ggaggtcccg gcggggtggg tgcccccgga ctgggagctc aagtacagcc ccatgacgta
    13561 cgatatgacc tgcgaggggc ccggatagcc ggaggagacg ggcctttcga aaccgagctg
    13621 aagataccgg agagggagca gaagcccgag gagatgatga cgaaggtcga ggtgaaggga
    13681 cggcgtctct tttgggggga tctagccccc cactaattat catctcagat cattttccgg
    13741 gattttcttc ggcaaagtcg ctaagcttcc ccccattcga cgagcccgac ccaaatttga
    13801 tatccagata tgcttcgacc accatttgcc tgaggtcttc ttcggattga acaggcaaaa
    13861 aaaggttaac tattaaagga ggaaacgaga aagctgtgct aaaatctaag gaggcggact
    13921 atgaacagag ttttttggat cgtggcaatg gcggcccttc tcgggaccac cgcggaggcg
    13981 ttcgatctga cgggagactg gggggcggaa ggcgcgaata tctacatccg acaggtgaac
    14041 gataccatct ggtggtacgc cgagaactcc gccgaggagc cgacctggac gagcgtcgcc
    14101 ttcgggaccg tcgagggcga cacggtgagc gtgaactggg ccgacgtgcc gaaggggaac
    14161 gccaccataa tgggaacggc agtcctcaag gtcttatccg aggatgagct tcagctggtg
    14221 gaccagaccg gcgggtttgg cggcgaggac tgggagggac tcaagctttt gaggatcaag
    14281 agcggtttct gatcccaacc catgggacgc accgctcttc tatttatcat cccatcgcct
    14341 gcccttttac aagcacgatg tcatcgatgc gacaggcatc ggatcacagg acgcactcgg
    14401 agcagtcctg gcagctgcac tcgatgttcg tgatcttcca cttgagggcc caccgcttca
    14461 tctccttgac aatctccacg atctcaaggc cgctctcagt cagggagtac tcggctctgg
    14521 cgggaaagga gcggccgtcg aacctcctct tcaccagccc ctcccgctcc agttccttca
    14581 gccgcgccga caggaccttc ggggtgatcc cggaaaggca gctcttcagc tcgctgaacc
    14641 tcctcacgtg cccctcgccc ttgtatagct ccagaaggac gaggagggtc cacttccggg
    14701 cgatgtacct gacggtcctg ttgacggtgc agccttcctg catggtatcc ttgtagaaac
    14761 cttctagcct atatacattg gtatcctaag tatatcatgt acgggcgatg gcgccctgcc
    14821 aggagctaaa tcatgaaggt cgtggtcctc tacaac
//
LOCUS       DASM01000107           20161 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_19174, whole genome shotgun sequence.
ACCESSION   DASM01000107 DASM01000000
VERSION     DASM01000107.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 20161)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 20161)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..20161
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_19174"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    5468..5521
                     /estimated_length=54
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3672 a         6245 c         6373 g         3809 t
ORIGIN      
        1 gggcggtctc cgccgcctcc aggaggggct cgggccgctc caggagctcg tccgcgaact
       61 ccgggtcgta ccggtcgacg tcggggaact tgatggagag gctgcggcgc aaggggtagg
      121 agtcggcaag ctccagaagc tcgtcccagt acctggaccg gatgaactct tcccacttcg
      181 ctacgggatc ttcgaccatg ggtaggggag ggtaagggag gggtttggtt taaagcttat
      241 tgtgcaagga agaatctaag cagaggacaa tgttatactt aaagaattct ttctttatca
      301 aaattaagag aattgatcta ttatacattt ttagagaaat agattgttca ataagggttt
      361 gaaggtgctg ctttaatttc gatcacttaa gaactatatt taaataattc attttgcgat
      421 gttcataacc tcataaatta tagattttat ctcaggagga atttggcttt gagaccaaaa
      481 ctgagcgatc tcttcaccca attttggttt tcctggtaat acatatccat ttttattgcc
      541 gatatgatag taataatctg atataatttt agatgtactt tttttcttat atctatttga
      601 ttctatatac tccgaagtta tccctattcc tggatcgatt gtctctagaa ttccaactaa
      661 tgcatcagat tcaacataat cctcaaattc gccctttgaa agaacgtgat aattattaga
      721 cttaaaatag ccttctctat ttaaatcagt tattatttcg attttatcat tgtcacatac
      781 cagataaaaa taatggtgtg ttgcggcaaa tacttttaat gtggatctca agtccaattc
      841 tgaatatttc agcaatttta ttctatgggc cactaaagaa tatccacttg atttgaataa
      901 tattggataa gcaacatatt cggtttcacc ctcaataagt aaatatactt catcttggct
      961 aaaaagtggt tcattcctaa aagaattatt ataccagtat actatatttc ctttacctag
     1021 ttcctttgcg acatctatct gcgaagctac gtatatatta tcacgagaaa atttgccagg
     1081 tattgatgct tctaccccaa tagcctgtac acctttcttt tttaagtcgt ctcctataaa
     1141 acttacaaaa aatgacatta tctgacatat atctgaataa atagccaccc tattttcaac
     1201 cttaggcgta taataaatca ataaagaatt ttcattaatt tttatatctt ttatatcata
     1261 ttgatctaag ccaaatgatc cagacttcaa tacaaacatc gccgcatcta atattttttc
     1321 tgcgccaatt tcatccaata aattcgtctt gtcattgctc aattttgtta actcctctaa
     1381 atgcaataaa gatatggatg tgatatgtaa caattcttaa tagtatataa aactaattca
     1441 aaccgctaaa actctcccaa agaaatcttt ctattattaa aatattaaaa ttttcatatg
     1501 gatatctgag acggagggag ttctgcgaat actgaacgca cccaccacca cctatcccct
     1561 ccgcctcacc ttcagcacca tcctctcttt tcccaccacc tcgaccgtct cccccgcctc
     1621 gatcggctcc tccgcctcgg cggcccagta ctcgccgttg tacatgacgt gcccccgggg
     1681 gtcgatccgg tccagggcct cggcgacctc ccggttcatc acctcggagt aggtgggcct
     1741 gcgccgcgcc tccgccacct tgtagagggc gaagacgaag aaggccccga tgacgatcgt
     1801 tggggcgatg agggcgacga aggcgtcccg cttgtaagag gcgggggggt accactccgg
     1861 cgacccgatg gggacgagga ggaccgaccc gatgaccatg caggcgagcc ccgccgctcc
     1921 cagaaggccg aagccggggc tgttcagctc caccaggagg aggacgatcc cgagggcgat
     1981 gaggaagagg gcccccaggt tgacggagaa gccgaggcct atgaggccga gggcgaggac
     2041 gatcaccccg accagctcag cgcccatccc ggggttggag aggccgaaga tgagggcgta
     2101 gaggccgacg atgaggagga gccccgccag caggggatcg gagaggacgg cgagaaccct
     2161 cagccggagg ctcggctcga agcggacgac ctcggcgccc ctggtggcga gggtcctatt
     2221 cttagcttcg gtgccgtcca ccacctccaa gagggccccg aggctggggc tgacgtgctc
     2281 gatgacgccg tatctcaggg cgtcctcggc gttgaggttg aggttcgaga ggacgaactc
     2341 ccgggccgcc gtcgtgttcc tgttgttatt cctcgccttc tcctcgacga gggcgacgac
     2401 ggcgttgatc accttggtgt cgttgaccgc ctccatcccc gtcggcccga tctgaaccgg
     2461 ctgggcggag ccgatgatcg tccccgggac catggcggct atgtccgaag agaggaggat
     2521 gatcgtcccc gcgctccagg cggtggcgcc ggaggggtag acgtacccga tgacgggaag
     2581 gctcgtcctc tcgatgaggc ctgtgatctc cttcgtctcg gtgaggcccc cgccgggggt
     2641 gtccagggtg atcaccaggg cctcgcaccc ccgcccctcc gcctctgcta tcgcctcccg
     2701 gacgatgtcg tcggaggccg gggttatggc gccatccagg gctaccacca tcacgcaccc
     2761 ctggcctgcg gccgagcctg ctaaagcgag gaggccgatg gataggaggg cgaggagggc
     2821 gaggtggaaa ggcagcggcc cgagatgggc cgcaggctct ggtctccagg aggagggccg
     2881 ccccaccttc ctccccttcg acggtgaggt attcatctct caccgaaggc cttggtgagt
     2941 cccgctacag cccccgtcgc ctcgccgacc tctccgccgg aggtgacgac gatcaggttc
     3001 ttctccctcg caatctcggc aagggtctga agctccctca gctttatcgc gacgggcgcg
     3061 tcctggtaga gcttagccgc ttccaccatc cgcgccgagg cctggaactc cccctcggcg
     3121 aggatgatcc gcgacctctt ctccctctcg gcctcggctt gcttggctat cgcccggagc
     3181 atcgactcgg ggaggctgac gtcccggagg gtcacagcgg tgatcttgat cccccagggg
     3241 tcggtctgcc ggtcgaggat ctcctggagc ttgacgttca gctcctccct cttcgagagg
     3301 aggtcgtcga gctcgatctc gcccaggacg tccctcaggg tcgtctgggc gaggaggctc
     3361 gtcgccacct tgtagttctc gacctgaatg acggccctgt ccggctccac aacccggtaa
     3421 tagaggatcg cgtcgacggc gacggtgacg ttgtccttgg tgatgaccgc ctgcttctgg
     3481 acgtctatcg tcacgacccg gaggtcgatg gtcatggcct tatctatcac cgggatgatt
     3541 atcgttagcc caggaccctt gatcccgctg tagcggccca gcctgaagat gaccagcctt
     3601 tcatactcgc gggggatcct gatgccctgg taaaggatga agagaactat gagcagaggc
     3661 aataagccta cggataatat gtctaccatg atgacaccct caagtagtta aaccccagcg
     3721 tttaaataac cgacgggatg ggggggagga tacaaaatca aaagggtgcg gcatgtcccc
     3781 ttcccaggga ggaaggggcg cgaaaaaagg tcacttcttg aactcggtat atccgcactt
     3841 tccgcaggcg agcctgtcct tgtgctcccc gaggaagacc ccgtttccgc accggggaca
     3901 gatctgcctc ttcctcttga tggcgtcgcc agcgaggtcg tagtactctt tcgccgccat
     3961 tctaacccga ctcctcggcc tgggctgcgg gggccgggac gttcctctct atgacgtggg
     4021 ccctctcgac ctctttcgcc ctctcctcgt tctcgtagat cttggcgtac ccgatcgtct
     4081 cgcgcttgcc gaactccgac ttcaagctat cgaccatcac cagggctacc ttcgagttca
     4141 tggtggcggc gatcttatct atgatgctct tcctggaggg ggtgggcccg tcgtgggata
     4201 cgacgaactt gacctctctc ctcttcaaca gagggttgtt cttctcctcg atgaccttga
     4261 tttccatctc aatcctccac tatcatcttg tccatgatgc tcttagcgta ttccttcctc
     4321 tcgggggtga ccaccaccgc cacgctcccc tcgtccggct ggccgtagac gaccgtcgag
     4381 ccgagggggg cgtagaggat ggcggggagg gtggcgagat cctcctcgcc gtcgacgacg
     4441 atcttgactc ggccgttgcc ggcgagccgc tcccttatca cgtcgacgag ctcctgggtg
     4501 agggtcgccg cgggattata gacctcgatc gtatcgtact cgtcgcggtc gccgatcccc
     4561 tgctggacgt ggtcggggac gggcatcctc ttcgtcttgt ggtcgacgat gcagatgtcg
     4621 ggcttccttc cggaagagga gaggaggcag aaggtggtga cgtcgccgac ggcgatcacc
     4681 ttgggggcgg gccggaggag gtcctgcatc ggccctatgc actcctgacc ccttccccgg
     4741 aagagggttc ccaggggggg cttcagctcg gagcggagct cttcgggaag acgcaatatc
     4801 ttcaaatcta ccgcaccttc agggcgtacc ttccgggaag ggttatcccc agcttctttg
     4861 cgacctccga ctccttcggg tcgatgatca cgacgtaccc ggtccagtcc ttgctaaggg
     4921 aggcagagcc gcagatcgga cagaccaggc cctcgacgat cctgtggcac tccctgcaag
     4981 cctgctctgt catctcgcct cgacctctgc ctcttccttc cgccgggccg cctccagcca
     5041 ctcgatcttc ccgaggcccg tctgcctcat ggtgaggccg atcttgctct cccggggctc
     5101 cttctcgttt aagctgatgg cgatgatccg cgcccggacc cggtcgccct cgccgagaga
     5161 tttgttcgag tccttgctcg cgagccgcga gttcttctca tcgtacgata tatactcgtt
     5221 ggttatctgg ctcacgtgaa ggaggccgtc gaagggcccg atccctatga aggcgccgaa
     5281 cttgacgatc tccaccacct ccccctcgac gatctcctgc atctcgggct tgaagactat
     5341 cgcatcgaag gtggcatcgt agtagaccgc cccgtctccc gccagaatcc tcccctcgcc
     5401 tatgtcgtcg acccccagca ccgcgacgac ggtgcccagg gtcttgtcca ccctcgcctc
     5461 gtacttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nctctggcgc aggtgtataa cggtaacacc tctagtagat aactttttct tcagttcctt
     5581 atcgttggtg aagaccgcgg cccccgattt cagggccagg tcgacgaggg cgtcgtccgc
     5641 ctcctcggat gcctcgagga tctcgcacct tcctgccaag gcgatgccga tcctggcggc
     5701 ggtccggtcc cgccgggatt cggcgaggca ggccaaggcc gcgagctccc ggaggacggg
     5761 gcgggggacg aggagctccc ggaaccccag ctcctccagc ccttcgaaga ggtccacccc
     5821 gaactggccg gggaccatga gggcgttggt gtccaggagg accttcatgc ggtcggagca
     5881 cccccccatc agcacgctgg agcctctact tattgatggt gccgacgccg atgagccgcc
     5941 accgggcgcc gacccgcctg ctgaccgcca cccggtcccc ggcggcggcg cataccggcc
     6001 ttctcagctg gacctccacc atccccccct ccctcgcgct cgatacgacg ccgacggtgg
     6061 tggcggtccc gacgttcagc atcaacggct cgctggtgtg gatcggctcg acgtcggcct
     6121 cgtcggtggc gccgacgacc ctctccagga gcctgatctt catggtgaag gagttccaga
     6181 cggggggcag ggtccccggc tctcccgcca cctgccccac cagaaggtcg ctcttggtta
     6241 gggcggggtc gagctgggtt ccaactccga tgaggccgcc gggggtcacc tcctcctggg
     6301 ggccgccgcc ggccatcagc gccgtcacct tcgtatcgat ggggacccac tgcttcctcc
     6361 cctccgactc caccgacctt ccgggcttga tctcgatggg gtcgtcgggc tggaggaccc
     6421 cctgggtgag ggtgccgccg atgaccccgc cgacgatctt ctccggcggc gtccccgggc
     6481 ggttgacgtc gaaggacctg gcgatcttta ggaggggcgg cttctccagg tcccttacgg
     6541 gggtcgggat cgtctcctcg atcgcctcga tcaccatgtc gacgttgacg ttctgctggg
     6601 cggatatggg gacgatggga gcgttctcgg cgacggtccc tttaacgaac ttcttgatct
     6661 cgttgtagtg ctcgatcagc ctctccttgg agacgaggtc gatcttgttc tggacgatga
     6721 cgatcctgtc gatcccggtg atgtccaggg ccatgaggtg ctccttcgtc tggggctggg
     6781 ggcagggctc gctggcggcg atgatcagga cggcgccatc catgatggca gcccccgaga
     6841 gcatcgtagc catcagggtc tcgtggccgg gggagtcgac gaaggagacg gtccggagga
     6901 cctcgctcgg cgatccgcat cccggacagg tcttctcgac ggtgtacgcc tccggcgccg
     6961 ggcagttggg acattttctg aaggtggcgt cggcatagcc tagcctgatg gagatacccc
     7021 tcttgatctc ctcgctgtgc tggtcggtcc agaccccgga gagggccctc accagggtcg
     7081 ttttgccgtg gtcgacatgg cccaccatgc ctatgttcac ggccggtttt tcgtgcaagt
     7141 tgggttggct ggacaaatcg ggttcctcga atgatcttga tgactcctcc ctgggagtga
     7201 ctactattta aatgctcagg actggatgcg gcggcctcct cgacctcgat atcatcgcct
     7261 gatcctgggg ccggtgccct ccacccctcg acagaggttt cgacctccct ggatcagcag
     7321 ggcggctcgg ggaggatgga ttatttggag ctgcggaata ttggggtctt acgggttgga
     7381 ttgaagagag atggggagga cgatgaggcg gatagatcca cccccccacc aaatgatctg
     7441 gatggtgccg atcagacgta cccccgatgg gcctccctca agccttcgac cgcctcccgg
     7501 acgaagaggc ggtaggaccg gatcgagaac tccgaccacc cctcgaactc gtcgtagagc
     7561 tcggccccga aggcgtcctc ctccgggtcc aagctcctgg tgccgtactc ctccagatcc
     7621 gagaagttct cctccagatg gaggaggagg gcggtctcgg cgtagggctt ggggatggcg
     7681 ggctgggagt gggatagggt cagcatcagc cgctcctcat cgatcaggtc ctccagggag
     7741 atcctcgcct tcttcaccga ggtctggtgt gctagctggc tgatctccag cttctccccg
     7801 ggggagagct tcctcatctc gacgaaggcc tgctggtaga gcctccccat ctcaaactcg
     7861 tgctccaggg cgtaccccag gacccggtcg gagacccgcc tcaggtattc ggagttgagg
     7921 gatatgaggg agaaccagag ccccctgaag tagtagcgtc ggttcatcgc cacggcgaag
     7981 tcgagggcgt ctgggctcga aggagagagg gaggggaaga aggtgacggc gtccgtctcc
     8041 accaggatgt agccctcggc ctcctcgccc ccgaggcggc ggatccgggc cgggtccgag
     8101 agggggcgaa acaccctcct cggctcctcc atctcccggc acctctggag gatccggtcc
     8161 cggtcgccgg ggctggcgga ggcgaggtgt tgggcgacgt ccttggccac cgagacgagc
     8221 cagcccctgt actcccgctc aaagccgtcc cggttaatct ccttctcctc cctcgaggac
     8281 ctcgatctta tccaggatct cccggaagga ccgccactgc tccaccggta tcctgatacc
     8341 gttcttcgac cagccggtgt acctatcgct ggtgacgtac tcccggatgt cgatccccgc
     8401 cgagccccgg aactcggtcc tacggactac cagctccgtc gtctcgctct ttctgatcct
     8461 cccgaactcc tgctccatag catcagctcc gatgggtatc gggcgccacc ttataaatat
     8521 ctcggcgtca tccttaaata tgatatatgc tatcgtgcgc catgttaaca cgaaggagat
     8581 tcggaattga gcgagattgg caagagggtg cggatggaga ggatcatcga taggaacagc
     8641 ggaaacaccg tcatcatccc catggaccac gggatatccg tcggccccgt caaggggctc
     8701 gtcaacctct cggagatggt ggacagggtg gcnnaacgcc gtcctccagc agaaggggat
     8761 ggtgaagcac ggccatcgcg gctacggaag cgacatcggc ctgatcgtcc acatgtccgc
     8821 cagcaccgcc ctcggccccg accccaacaa taaggtccag gtctgtcagg tggaggaggc
     8881 cctgaaggtg ggggccgacg ccgtctccgt ccacatcaac atcggctctg agaccgaggc
     8941 cgatcagctc aagaagctcg gaagcgtcgc cgagaggtgc gacttctggg ggatgcccct
     9001 catcgccatg atgtaccccc ggggcgacaa gatcgccgac cccaacgggg tcgacgtcgt
     9061 ctccctggcg gcccgggccg gggcggagct gggggccgac atcatcaaga ccaactacac
     9121 cggcgacccc gagaccttca gggaggtcgt cgccggatgt cccgtcccgg tggtcatcgc
     9181 cggcggcccc aagaccgaga cggacgagga gttcctccag atgatccgcg gcgccatcga
     9241 cgccgggggg cggggcgtcg ccatcggaag aaacgtcttc cagcacgaga acccgacgaa
     9301 gatgacgagg gcgatatccg ccatcgtcca cctcgaccgg accgtcgagg aggcgatgga
     9361 gatcctgagg tgaggcggga ggaggccgca aggacggccc ggtcttccgg cgggagcggc
     9421 ggaacggcgg gagatccgat ggcagcgccc cctctcctcc ccagggggtc tggggggggc
     9481 gagacctcaa atcgacctca cggtcccggt tcccgatcga tcggctttca tccctcgcgc
     9541 ggaagggggc gatgcacaag ctttatatga tcggagcggg tttgggaact gtcgttccca
     9601 tgccagcgaa gcggggtggg gtagtcagga aatcccgacg ggctcataac ccgtagatca
     9661 gtagttcaaa tctactcccc gctaccaaac acaattttga tccgtctttc aaaagttagc
     9721 ctcgtcttac tctcaagtcc atttgggcgc aagataagat caaaaaaaga tgtggttgag
     9781 cctgagccca tccatcgaac cctctcccct ctttctctct tcggaaaccc tcagattcac
     9841 cccaccacga cgacgttgct cgcccccatc agcttccggt ggacgttatc ctcgaagagc
     9901 cggaagtcgt acctccccgg cgtccggggc gcggcgaagg tcatcgtccc gcaggccctg
     9961 ccgttcgtcc actgcatccc gatcatgtac ttgtcggggc gggtcatctc gtacatccct
    10021 atcgccgagg cgggcttcgc cccccagaag gagactgtga tggcctcccc ggggcgggcc
    10081 gccgcggggg acgcctccac cctcaccccc gcggagacgg cgacctcgat aggctggctg
    10141 accgcgatcc tcgtgaatcc gtcgtcctca aacatccgaa actcgtagct tcctgccgag
    10201 gaggggatga cgaaggggag ggttccgcgg ctcgttccgt ccaggtactc tcttgagatg
    10261 ggtcgatcgt ccccggcgcc gacggggtag aggccgatcc agtccccttc aaagccgggg
    10321 gctccgctgt agctgacgtt cacccggtcg caggtcccgg ccgaggcgtt ggcgactttg
    10381 acctcggggg tcgccacggc gaaggggagg gatacgacct cggagccgga gccgtcctcc
    10441 attctcagga cgtagctccc ctcaccctcg ggggcggtga aggtgacggt cccgctcctc
    10501 cccgccaggg gctggctgga gagggccacc ccgatcggct ctgtatccgt ctccatatcg
    10561 gtcttcggcc ccgtcccggc ctctcccgtc atcggtccag agccggggga gggggctgca
    10621 aggaccgccc gcccggtgaa gttctcgggg gcggcgaagc agaggggcat ctccgcgccg
    10681 ggggagaagg tcgatcggta gaggaagagc ttgaagtcgc cggcggaggc ggcgtacttc
    10741 ctgacggcgt cctcggaggc gatcggccgc tcgtcgacgc tcgggccgcc ggggatcttc
    10801 cggggggcgt cgcagaggcc gtctctgtcc ctgtccctgc acccctcctc gatggcgtcg
    10861 aagtcgtcga agtggtttcc tcccctcccg tcgtcccagc ggttctcgcc gtcgtcgagg
    10921 gcgttctcgc cgttatccag gagctcgttg ccgtagatcc tgttctcctt cagccctccg
    10981 gcaaagccga tcttgctcac ctccagggtc gcagcctccg tcttaggcct gtactttatc
    11041 gatacgccgc cggagtcgga cgcctttatg ctctcagacg cgttctgcct cacagctttg
    11101 atcccgacgc cgttctcggc gatcctgttt ccggcgatct cgttatccca gctcctttcg
    11161 aggaggagtc cgatcccgtt cccctcgacg gagttgttcc ttatggcgcc gcccgtgacg
    11221 ctctggagga ggattccgat cctgtttcct gataagtggt tatcctcaac ggtgcacccc
    11281 tcccccagga cccggacccc ggcgtccatc tcctcggagc cggagccggt gaccaaaaag
    11341 ccctggatcc gcgccccctc cccctggagg gtcacccccg atccagagcc tccggcgtcg
    11401 atctggggcc ttccgctccc ctggagggct atcccctccg atacgaccag cctctccctg
    11461 tagactccgc cctccaccag gacggcgtcc ccggatctcg cctcgtcgag ggcggcctgg
    11521 atcctctccc cgggggcgac ggtgagggtg gcgcccatgc cggtggccgc caagaagagg
    11581 gttgagacga tcgatgagct gatcagaatc gctatagcca gacgggaagg tcggcggaga
    11641 cggccgccga cagaaacccc ctcatgaccc caggggacgg atcggacgct tctcggcatg
    11701 atccccgcca gggggccccg ggttataaaa ggttttgagg ctccgccgcc ccccccgggg
    11761 cggaggatcg cggcgggaga tctggggagg ggttgatcct ctccccaccc cccggctcac
    11821 atcacggggc gcgagtagat cgtatcccgg gtgtagccga tcacctggac cgtcctcctc
    11881 ttcggagcct cctcgacggg gggcgccgta gccccctccc ggaagtccga gatccccccc
    11941 gcgacggcgg ggacgaaggg gaggggctcg ggggcggccg gctcataaag ggagaggtcg
    12001 ggggtcgtca gcctcccgga gaagttcccc ctccagccgg agccctcctc gtcggaggag
    12061 acgaaggctc cggcgatatc cccggatccg tcgacggtgc cgatcatcct tccgaccacc
    12121 atcggatgct ggatcagcag gaccaccagg tcgatttttc cctcctcaag ccgccccgtc
    12181 accatcccgt tccaggggtt aggggattcg ccggtgcagg cccccgagat ggcgggcccc
    12241 tgcgaacgga tcgccgccgt gaactccgcc agggggaggg gtttagagag ctgatcatcc
    12301 tcgcaccaga aggcccagat ccccgtcagg tcggctccgg cgacgccggc gatccccaga
    12361 agtgctgcca ggatcaagat ggtttttccg atcatccgtc ccccccttaa tcccccggat
    12421 ttattaaggc tatcccgggg tacggaaagg gtaaatcgac ggaggttcaa ggaggcggag
    12481 aggcgatcat gaccaccatc agggacccga tccacgggta cgtcaggctc gacgatctgg
    12541 ccatcgatct cgtcgacacc caggagatgc agaggctgag gtggataaag cagctgggtc
    12601 tggcacacct cgtctatccc ggagccaacc acacccggtt cgagcacagc ctcggctcct
    12661 accacctcgc aggccttctt tcgcgacacc tcggcctcga cgaggcggat gggacggaga
    12721 tccaggccgc agccctcctc cacgacatag gccacggccc cttctcccac gtcaccgagc
    12781 gggttttatc cagctacctc cgggaggagc acgaggatgt cgccgaccgg ctgaggaggg
    12841 gggagctggg ggacgtcctg agggaccggg gcctccagcc ccaccggatc ttgaggctga
    12901 tccgggggga gacccccctg gggcaggtgg tcagcgggga ggtggacgtc gacaggatgg
    12961 actacctcac ccgggactcc cactacaccg gcgtcgccta cggcgtcgtc gattatcaga
    13021 ggctgatgga gacgatggcg atgaaagacg gccacctagt cctcgaggag gggggggtcc
    13081 acgccgcaga gtccctcctc gtctccaggc tcctgatgta cccgacggtc tacttccacc
    13141 acgcctccag gatagcccag aagatgctgg atgtaggggt ccgggtcatg gtcgaggagg
    13201 ggtccgatcc ccgaaggatc cgggagatgg acgacctcca gctctccgcc gccatggcct
    13261 cgacgggggg ttaccccggg gagatcatcg agaggatcag ggcgaggagg ctcttcaagc
    13321 gggcgatata cgtgggccgg gaccggctcg aggcgccgga gaagatgggg aaggagggga
    13381 ggatcgccga ggagatcgcc gagatcgccg gggtcgatcc cctctacgtc ctcgtagaca
    13441 accccggcct cccccggata gcggagggga acgtgggcgt agtcggggtg gacggggagg
    13501 agaggcccct ccgggaggta tcccccctcg tcaccatcat ggagagggcc cacctcgccg
    13561 cctggaggct gggggtctac accaccgagg agcaccggga ccgggtgagg agggcggcga
    13621 catctcatct gaaggtcggg aggaaccccg tccagcacac cttcgaggac ctgtgactcg
    13681 aggactcgtg agataatagg tcgggagaac ctttaagacg gtccggggcg gaaggggtag
    13741 ggatggtcgc cgttgacaac aatctgannt tcagtcgatg aaccagcacc ttcccgggag
    13801 gaggaagagg ctgacggagc tcctccagga ggagaagccc ggggtccggg ggaaggacaa
    13861 caccttcttc atcatggaca aaaaggagct gaaggtcatc tccgaggcgg tcccccggta
    13921 cctctgggac aggctgaggc tcccgatcct catcgagatg gccccccagt acgggagcgg
    13981 ctcggcccgg gtccaggggg agnntggtga gaaagctcct caagatcgag cggggcgatc
    14041 ggaagatggt gatcatctac atgcccgaga tccgggagct gaggcgaaac ctccccacca
    14101 ccagccagta cgccttcatc accgccctgg actgatcctg atcttttttt atacgatgcg
    14161 gacatattat agcctcggag gtgtttgacg atggctgaaa ttgctccggc cgaggcgatc
    14221 cggctcttcg aggagaggat ggcccagggc cggtatcggg aggcggcaaa gatcagggag
    14281 gattactccc tatccgccga gccgctccag gatgccgtca ggagggagta ctcgagggtc
    14341 ttgggcctag gcgagtactc cctggcggcg gagctggcga aggagtacgg cctatcgaag
    14401 aagatggtcg tcgaggctgc gtccaggtcc tttgtgagga aggtcgacgg cgagcagtac
    14461 aaggcggcgg cggagttcgc gaagaagtac gacctcccgc cggagatggt gagggaggcg
    14521 gcaatccggg cctacaacaa gagcatggac ttcggcctcg ccaagaacgc cgccgatata
    14581 gccgtcgact tcgagctccc cgacgatatg aggatagccg ccgccgagaa ggccttcgcg
    14641 gcgcatatgg actccggcct ctacaacaag gccctgaagg tagcccggga gtacggcctc
    14701 tcccccgacc tcctccggga ggcggagacg aaggcgaaag ggcggaggtg atccatggcc
    14761 cccgtccttc tcaccgtcgg cggctccgac tcctccggag gggcggggat ccaggcggac
    14821 atcaagacct tcggagccct cggctgtcac gggacctcgg cgatcaccgc cgttaccgcc
    14881 cagaacaccc tcggcgtcct ggaggtctac cccatgcccc ccgaggcggt catatcccag
    14941 cttgcggccc tagccgagga cttcgagatc tccgccgcca agacggggat gctctcatcc
    15001 cgggaggtgg tcctggaggt cgccggcttc ttcgagaggg aggagatccc cctggtcgtc
    15061 gacccggtgg tggaggcgga ggcgggagga cggctcctcg atccggaggc cctcggcctc
    15121 ctcgtcgaga ggctcatccc cctcgcccgg gtggtcaccc ccaacatctt cgaggcggag
    15181 gcgttgacgg ggatccgggt caccgacgag gggagcgccc tccgggccgg ggaggcgatc
    15241 ctcgacctgg gggcggaggc ggccgtcgtc accggaggcc atctcgccgg agacgacctt
    15301 ctggtgacgg cgaaaggata ctgcatcatc cggggcgaga gggtcccggg aggaaaccac
    15361 ggcgtcggct gcacctactc ggcggccaca gccgcatttc tggcgggggg tcgggatatc
    15421 gaggaggcgg cgaggctcgc gaaggccttc gccgccggct cggtgagaaa gagcatcgac
    15481 gtcggcaggg gcgcctcccc cgtcaacccc ctcggcgaag ccctcgagag ggcggaccga
    15541 tacgccgtct ttacgggggt ctccgacggg gtcgaggccc tcctctcgga gccggccctc
    15601 gtcgatctga tcccggaggt ggggttgaat ttggggatgg cgatattcgg cgcctcgggc
    15661 cccgaagacg tcgccgccgt cgaggggagg ctggtgaggg cgggaagaag ggtcagagca
    15721 tgtggatcgg tccgattcgg ggcgagccgc cacgtcgcca ggatcatcct cgcggcgatg
    15781 agcttcgatc ccggggcgag ggcggcgatg aacctccggt tcggcgagga tgtcctggag
    15841 gcggccctcg ccctcggcct ctcggcgtcc gatttcagcc gggaggagga gccggaaggc
    15901 gagaagacga tgagctgggg gacgggggag gcgatacgcc gcttcaggtc cgggtcgaag
    15961 ggggggggtg cgaccgatct gggccggtta gagcggccgg tcccccaggt catctgggac
    16021 cggggcggcc ccggaaagga gcccatgctc aggctcctgg gccgatccgc cggcgaggtg
    16081 gcggatctgg cggtggagat cgccaggggt ctatccgcca gacgggaacc tataaattga
    16141 agtttggtgc tgtaacattg cggttcttta tttggagtgt cctatatggg aagcgtaaag
    16201 cctaactaca tcaagaattt tgcagatgac ctgatgaagg cctaccaggg ggtcttcaac
    16261 aacgacttcg agcataacaa ggatatggtc tcggagtaca ccgacatcaa gaacaagacc
    16321 atccgcaacc gagtcgcggg ctacataacc cgcaaagccc agatccgagg agagtaaggt
    16381 ggatgtttga aggtgtgctg ccggccatca tcaccccctt ccgagaggat ggagaggtgg
    16441 acgaggaggg gctgaggggg aacgtcgagt acttcagcgc ggccggcgtc gctggcatcg
    16501 tcccctgcgg caccaccggc gagtctgcca ctctcaactt cgaggagcac aaaagggttg
    16561 tcgagatcgc cgtcgagtcc gccagggtta aggtggtggc cgggaccggg tcgaacaaca
    16621 cccgggaggc cgtggagctg accaagcacg ccgaggacgc cggcgccgac gccgccctcc
    16681 tgatcacccc ctactacaac aagccgaacg accgggggat gctcgcccac ttcagggcgg
    16741 tggccgagag ctccgacctt cccctcatcc tctacaacgt cccggggagg acgagcatca
    16801 acctcaagcc tgagctggtg gccgagctcg cgaagctcga gaacgtcatc gggataaagg
    16861 aggcgagcgg gaacctatcc cagatctcgg cgatcatcga gctgacccga gacgaggagt
    16921 tcttcgtcct ctctggggac gacggcctca ccctcccgat catggccctg ggggggaagg
    16981 gggtcatatc ggtggtggca gacgtggctc cgaggaagat ggtggcgatg gtggatgcga
    17041 tgctgaaggg agacctcgag aaggcccgga ggctccatta catcctctct cccctcgtct
    17101 ccgccctctt cctggagacg aaccccatcc ccgtcaagac ggcgacgagg atgatcggcc
    17161 tcgccgcagg acccctgagg ctccccctgg cgccgatggc ggagaagaac gagatgaggc
    17221 tgaaagaggc cgtggacctg ctgggcgagg tcgcatgacc gtcaggatag cggtggcggg
    17281 gtcccggggg cggatggggc agcttctcat cgaggagatc gggaggtttg aggacctctc
    17341 cttggtcgca ggcttcgatc tggtgggggt gggcgagccc ctcggcggcg gggtcttcgt
    17401 ctccgacccc cgggacctgg ccgaggttct gaggagttct ggcgcccagg tcctcatcga
    17461 cttcaccgcc cccgcggcca ccgtcgagaa cgttcgggtg gcggcggacc ggggggtcaa
    17521 tctggtggtg gggactaccg gcctatccga cctccagagg cgcgagatcg ccggatcgat
    17581 cgaggagagg gtggcggcgg tgatctcgcc gaactatagc gtcggggtca acctcttctg
    17641 gaggctgatc gaggatgccg cgatctccct ggagggctac gacatcgagg tgatcgaggc
    17701 ccaccaccgg aagaagatgg acgcccccag cggtacggcg gcggaggcgg tccgggtcct
    17761 ggcgggggtt accggcaggg acgcggtcgt ctacggcagg caggggcagg gtctccgggg
    17821 ggacgagatc ggggtccacg ccgtccgggc cggcgacatc gtcggcgacc acaccgtcct
    17881 cttcgcgggg cccggcgagc ggctggagat taagcatcag gcccacagcc ggcaggcctt
    17941 cgccaccggg gcggtccggg cggcgaggtg ggtagcttca gccacccccg ggatctacac
    18001 catggccgac gtcctcggtg agaggtgacc tgtccgcagg ttgggttaaa tgcgccggcg
    18061 ttggagagat gtacagcttc gacgagagtg agagagagct ctattttgag ggggagaggc
    18121 tcgataggcg gtggacgggg gaggagctcg ccgggttctg cgccagctgc ggcggcgagc
    18181 tctcctccct cggctgttac agcacacccg gagggtggct ggtggcgtcc cggtgccggg
    18241 gctgcggctc catagtcctt gcggagtacg attcccgctg ggcgtggaag ggggatcgag
    18301 aggtagcagc ggcgccgccg acccccgggc ctcggaagat gccgaggatc tccgagattc
    18361 cccgggagga gctggaggcg gtcttcaccc ccgcggagct gagggacatg gagcggtgcg
    18421 agcgggggga gccctacctc cgccagaacc tgtaccgggc ccgggccaag taccagaagt
    18481 tcgagaggct tttcggggtg aggatcgata tctgaaggcg atatgccctg ctcggggccg
    18541 aggatctgag gtgggcggca gaggtccacg gcctccccct cgggggagat catggccggg
    18601 catcaagggc tgatggtgag gtggcagccc gacgcccaat aagggccgtc tatggtttta
    18661 gccatcaaaa aaggtataaa ggaaggactg ccggtcctgc cgggcgcctt cagaatatcg
    18721 ctgccagagc gtagagcggc agcatgaacg tgatcagaaa ccctacgatc ataagcccga
    18781 tggcaactcc attttctccc atgaatctcc tcacctctcc atcttcagcc gattggcagt
    18841 aaggttggga gaggtggtat taattactat cggcgggtct caaccccgag ctgccacccc
    18901 acccgtccga taatcttaac cccggtggcg ctgatttggg tccaccttcg gggaccgatc
    18961 tggtactggg acgacggcgg cgcgatgatg agccgacctg cctgatggat gatgaggatt
    19021 ttgagtcctg agcgccatcc atgcttcttg atcagaggtc ccggtggggg atcaggtcgc
    19081 agccggtgca ccgatagcac cccgtctcgg cggcgtcgcc gcggaggcgg tgctcgtcga
    19141 gggcccgccg gaggtggcgg gagaggtcga ggagcctctc cggagaaggc ctgaccatct
    19201 cgacctcccc gacccgcagg ggatgggggt agacggccct gatcaccggg agggccccca
    19261 tctcggcgag ctcctcgatc ccctcccgga gggagggctc cgattcgccg aggccgacga
    19321 tgacgttgga gaagaccctg tttctcccga accgggctga ggcgtcggcg agggccgcct
    19381 tgatcttgtc gagggaggcc ccggggcaga cccttccgaa gaggaccggg tcgaggcact
    19441 cgacgttgta cttgatctcg tccgcccccg cctcctggag agccttgctg gaccctctgg
    19501 cgggggtgac ggagaccccg atgggtaggt cgaacctgcg gagggcgcgg acgatctcgg
    19561 cgaccctcgc cacctcctct tcgggggtcc gctcgacccc gctggtgagg gatatcgcct
    19621 ccagctcccc cgaatcgagg gcctgcgcca ccatctccac caccctctcc ttcggcttca
    19681 ccccccccga gagcttcggg acggcgcaga acttgcagtc gtnngtaggc ctgccggggg
    19741 cagtggagga ggggcctctc caggacgccc cgggcgatct ctctgccctc aaaaaggata
    19801 gccacctcct ccccctccag ctccaccagc ttcagggggg agccctcctc ggcggagagg
    19861 cgcaccctcc gcccgccgga ccggaagaag acgttcctct ccccggcgcc gggtcccgcc
    19921 gtcgagaccg gcccctcggg gcggaagacc tccggcatct ctatcctgcc ccaggagatg
    19981 aggcgggcct tggtcagtag atccatcgcc atcatgtatc ccccgggggt ttatagggat
    20041 ggccccaggc gataggaggc tggaggtggg ggccgaaatt ccccgcctac aggcagggga
    20101 gccagtccac cgccccggtg gtcccgctga cgttgctgaa gagctggatg tacttctcta
    20161 t
//
LOCUS       DASM01000108            4920 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_189388, whole genome shotgun sequence.
ACCESSION   DASM01000108 DASM01000000
VERSION     DASM01000108.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_189388"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          931 a         1599 c         1446 g          944 t
ORIGIN      
        1 ggtccgggcc tgctcgtccc gctcggccac tcctcggtcc cggggaaggg ggcggggttg
       61 ccggtacccc agaaggttat cccggtctcg acgtcaacgg cgggcggata ccaggcgcct
      121 ccgccgctgt taaccccggg gttaccccag aggtccggcg aatctacggt ggagaagctc
      181 cagatgacct ccccggtctc ggcgtccagg gcgctgatca ccccgatccc ccccggggcg
      241 tagaagacgt ccccggtccc cgggacggtg gagacgtaga cgaggccggc gtagacggag
      301 ggctgaatgt cgatccccgt ggtcgcaacc tccgagagcc tcgtcgtcca gacctcctcc
      361 ccggtcccgg cgtcgagggc ggtgacgttg tagaggtctt ttgctacgaa gaccttcccc
      421 cagccgacgg caggtccgtt ggggccgacg acctcggtga cgttgtagaa cctcgaccat
      481 atggggtcgc cggtctccag gtccagggcg aagacgttac acctcaggtc ctggaagtag
      541 acgacgtccc cgacgatgat ggggttagaa gccgcggcgc cgtaggcccc gatccccggt
      601 atgggaaacg accagaggac ggccagctcc ccgacgttct cggagtttat ggcggagccg
      661 gcggccgccc tggtgttctc atagtccccg ttggggaggg gccagtctct agccccgtcc
      721 gacacctccg ggggaatcgg acctcctccg gcggggctgg cggcgagagc cgccacggcg
      781 aggatcaggg caaagatccc cgtcgccgtt ccgatcgatc ttccgaattt tagcaaggcg
      841 atcacctctt ttacctctta actctctcta tactctcctc atttatcttt tctttcatat
      901 aggcaggacc aatcctcgga tggtccgggg ggcgatctcc tggaggatgg agaggtctct
      961 ggacgggtgg accgcggagg gttcctcccg gcgcggggcc ttcaagggac gtttgcctgc
     1021 agcaaagcct ccccgggaag aaaagcttaa atcgattcgc ggagacatat agaggcctat
     1081 aaggcaaaaa gtgggaggtt agtcatgaaa tgctctggat ttggaggaat cgtccttgtg
     1141 gcctccgccc tcttcgtggg gcttgcggcc gccgaggatc tgacccccga cgatatcatg
     1201 ctggagacga agaacatgac catcaccggc ctcagctacg ccgacgatga cgaatgggtg
     1261 gaggtggtga accccgggat ggcgagccag gacttcacct tctggaccct ggaggatgag
     1321 aacaacaaca cctacctctt ccccgagggg ttcgtcctcc tgccgggagc ggccgtgaag
     1381 gtccacaccg gcatgggtaa cgcgacggag accgatctct actggggtcg cgacgacctg
     1441 gtatgggacg atgaagaggt ggcgaccctc aagaacgctg ccggccagat cgtcgcccgt
     1501 tatccggaga gcgcctgagc tcagaagagc cctcgccctc cttcctattt ttcctcgccc
     1561 catctccgtt gcgcgatgtt gttctcttcc acccttcccg tttctggggg gcgtccggtt
     1621 tttctttcaa acttccgagc aatcgaaata ctatatttaa aaacttgaat atatctaaaa
     1681 tagaagaagt aactgtcgat tttatagcca tttagcgcca taataggcca ctatatcttc
     1741 gatctcgcct cttcagacta attgacaatt tttgagataa ctttatatct tttgagaatg
     1801 agtacttctc tcaggaggta tagaagatat gaactccgta tcgatggtac tgaggctgat
     1861 cctgacatcg gcgctggcaa tttcggccgt agggatctcc tgggcagagg atgaggaagt
     1921 cgggatcgat gctgaaatgg agggcgttta cggcgtcgtg atcgagaacg tcagctgggt
     1981 cgacgagtgg gtggagatat ccaacctcgg ggaggctgcc caggatctta cgggctggac
     2041 tctgagggac gagcagaacc acacctacac cttccccgag ggtttcgtcc tgatggaggg
     2101 ggtatcggtc ctggttcata ccggcgtcgg tgacgacacg gccaccgacc tctacatgga
     2161 tctggccagc cccatctgga acaacggcgg cgacgtcgcc accctgctgg accctgacgg
     2221 gaacgtcatc tccaccttca tcagtccgga ggaggagggc gaggagtagg atgaaggggg
     2281 cggaaaggcc ttgatctgat ccggcatccg atggagatcg ggatcgctga gatgatcgac
     2341 ccctcctcct cggatccttc tcccgtctct ttttggcggt gctccgcgga ggctcctgcc
     2401 acatatttta attacaataa atcaataaaa actttctgat agccttcacc agaaggcccg
     2461 ggggtctccc aaacctcctc ctgcccgccc ctcccgggac gagccccctc agatccagcg
     2521 gcgaaccctc ctcctgtagt cgaggtactc ctcgccgaag gcctcctcca gcatctcctc
     2581 ctcccttcgg atgaagagcg cctccatcag gcccgcgaag atcaacggga aggagaagct
     2641 ggcgagagac ccgaagacga ccgcctcgcc gaggaggatc gtggccatcc ccagatacat
     2701 cgggtggcgg ctgatccgga aggggcctga gattacgagg gcggtgggca tctctcgggg
     2761 cttcaccgtg gtcttcctcc tcttgaagag agaatccgcc cacaggttca agatgacccc
     2821 gaggccgatg agcagaaatc caacaaaatc ccacggaggc tccgccacct gtcgcgttgg
     2881 atatgagtag tgtaacgcgg cggataggac gaggagggcg gcaaagatcg tcgggggctg
     2941 gatctcggcc ttcatcctgt caaaaattat cggacccggg ataaatatct tatccaggct
     3001 ttcgcccggc gtccatctcc atcggacccg cgggggatga gagcgtcctc gaggatatgg
     3061 acctgaagcc cgccctctcc atcgaggagg cgatctcccc ccggtcgaag gcgatctcgc
     3121 ccctcagaag ctccgccggg agccatccga gcttcatcgc cgtcggcttc gtcctctcgc
     3181 gcgtgagacc gtcatggagg ctcacaaaga cgccgccggg gttcagggcc tcccagatct
     3241 ttctcataag aggatcgagg ctgcccctgc aggagtagag ggaggcggag gcgaggacga
     3301 ggtcgtactc ccctccgatg gcgtcctggg agaagtcccc ggcgaccacc tccacccgcc
     3361 cctccatctc cgcctcccgg acgaacctct cggcagcctc cgccaccggc gggaggtcga
     3421 agactacgcc cctcatctcc gggtgggcgg ccaccaccgc cccagagatc agccccggcc
     3481 cgccccccag gtccagcatc ctccggaagg atggcgcctc cggaagctct ccgatcgccc
     3541 tcgccaccat ctcggcgacc ccggtccgct ggtactcggc gacggcgtcc gcccaacggt
     3601 ccgccgccgc cgacgtcggc tcagccgcca tcccctctcc cggcgggcct tcgacgacga
     3661 gccggggcat ctcctcaagg gccggctgta tctgccgcca gacctccacc agatagcctc
     3721 cgacgggggt ggggccatcg cgggcgagat gggtcgctgc gatccccgag ttctggtaga
     3781 gcccctcctc cttgacgacg aggtcgaagg cggcaaggcc gtcgaggaag atcctcgtcc
     3841 cctcgggaga gccgccgagg atctccgcca gctcctccga cgacctcttc tccccgaggt
     3901 ggtcgaagac cccaagctcg atcccggtga ggaggagccg cgtcttgatg ggggcgatga
     3961 ggaggtcgta aagctccgag aagcctgcga ccgcctcctg catctccgct ccatccggtc
     4021 tcatctggca gccctcgaat agaccgtcct cccgtcgtac cagatccgga ggatgcggtg
     4081 gtaagggatc atcgtctcgc ctcgggcgaa gaaggacctg ccgagggaga tctcgctccc
     4141 cttcagggtg atcctgtcct tcggtgcccc ccggtggagg acctcgatct cgcacctctc
     4201 catatcgagg ccccgccact tgatctcgtt caggatctcc ctggggtgtc ttctcccctt
     4261 atcaaactcg gccatcctat ctcctttccc cctccccata tccctctcat cctattatcc
     4321 ctctccctga gaaagccttt taggggatcg ggctattcgc tcatccatcc cggttaattc
     4381 ggacttgatc gaaatgttcc ccgaggacgc ttaccatctg gagttcttca aagacgaggg
     4441 cttcacccgg aagacctgcg agaggtgcgg cggcagcttc tggacgaggg acccctccag
     4501 gaccacctgc ggagaccctc cctgcgatcc ctacaccttc ataggatcgc cggtcttccg
     4561 ccgggagatc ggcctggacg agatgcggga gcattacctc tccttcttcg agggaaaggg
     4621 ccacacccgg ataaagaggt atccggtgat agcgcggtgg agggacgaca tctacctcac
     4681 catagcctcc atcgccgact ttcagccctt catcacctcg ggggaggtcc ccccgcccgc
     4741 aaaccccctg acggtatccc agccctgcat caggctcgac gacctcgact ccgtcgggag
     4801 gagcggccgc cacctcacca ccttcgagat gatggcccac cacgtcttca acacccccga
     4861 ggaggagatc tactggaagg acaggaccgt cgccctctgc gacgagctcc tcctgggcct
//
LOCUS       DASM01000109            6065 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_101849, whole genome shotgun sequence.
ACCESSION   DASM01000109 DASM01000000
VERSION     DASM01000109.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_101849"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1173 a         1974 c         1953 g          963 t
ORIGIN      
        1 tggaagaaaa gaaggcccgg ctgaagcaac aggccgtcga ttataaggaa agtcgcagcc
       61 aattgaacgc tgaggcgagc aagtgggccg cggagcggaa tgaactgaac aagaagacca
      121 aagagctcat agacaaagcc caagagctca agacgctccg tgacgagtac aacaagaacg
      181 tggctgaggc caaactgaag cgggacgagt acaacgagaa gaccaacgac ctttacgctc
      241 agatagacga gatcaggaag aagcacaacc tcaccggcga tcgatcgatc cggggtctgc
      301 gtcgagagat agaccacatg gagttcaaac agcagaccga ggttctcagc cccgacaagg
      361 agaagcagct ggtcgagaag atctccgccc tccgagccga gttcaagacg aggaaggagc
      421 agatggagaa gaacgaggtc ttgagagacc tcttggacga ggcccagaag ctgagggacc
      481 aggcctccga gtaccacgat ctcgtcacca agtccgcaga gctcgcccag gaatatcacg
      541 acaagatgat cgccaccttc aaagaggccg accaggtgag ggccgccgcc gacgccgccc
      601 accgggagtt cgtcaaggcc caggaggcgg ccgacaagca gcaccgagag ttcatcagga
      661 ctcagagaga gatacgtgat ttcgacaaga tcatcacggg tctccgaaag aagaaccggg
      721 agaagaggga ggacaaggtg aaggccgagg tcaggaagga ggccgaggag atcctcaccc
      781 agttccggca gggagagaag ctggacaccg ccgacctcct gagactccag cgttcaggtc
      841 tggtttaatc cagcataaga gggcagcatt gcgggggggc cccaggcccc cccaaaaacc
      901 ctgcatattt ccgtcgccct tcggcgatcg ggcggcgacg gccccaggtg gacggagatg
      961 aagataacct ggctttctca ttcttgcttt gaactcgtcg agacgatgag ggtcctcatc
     1021 gacccctacc tccggggaaa ccccgccgcc ccggcgtccc ccgaggagct cgacccggac
     1081 gcaatcgccg tcacccacgg ccacgccgat cacctagggg acgccgagga gatcgcccgc
     1141 cggactggct ccccggtcct ggccgtctcc gaggtggcga ggtttttgga ccgccggggg
     1201 gttcgatcgg aggggatgaa catcgggggg accctccgga tcgccgacgt ctccttcacc
     1261 atgacgaggg cggtccactc ctccgggatc aacgtcaccg acccgcctgc ttgcggcggc
     1321 gaggctgcag ggtttgtcat cgaggacggg gtccgggtct accacgccgg ggataccgcc
     1381 ctcttcggcg acatgaggct catcggagac ctcctccgac ccgaggtggc gctcctgccg
     1441 atcgggggga ggtacaccat ggggccgagg gaggcggcga tcgccgcgag ctggataagg
     1501 cccaacctgg tcatcccgat gcactacaac acctggcccc ggataagcca ggaccccgcc
     1561 gagttccgag atctggtgga gactctatgc gattccgagg tcgtgatcgt cgaggctggc
     1621 gactgcgtag agtgctgaag ccccgggggg gctggaggca ggagttcgtt tttatattaa
     1681 tatattttta tatttccact tccttctcct ggccctccct gccatcctgc cgatcctgcc
     1741 cccccggggc gaccgcaaac ttttaaactc cgtcgccgaa ccaaccgtcg atgagcgatc
     1801 tgcgcctgct ctttggaagg cctaagatga aggggacggg gaagtttctt gcggcgttga
     1861 gggaggctgg ggagggccga ggctgcatca tccaggcgat ggacgcctcc ctcgtggtcg
     1921 gcgaacgcca cgccgccttc gccgccgaga aggcggtgcg ggccttcacc gagaggagga
     1981 acgtggccaa agacctgggg ctggagatcc tccgctacgc atcgggccag agacagatcg
     2041 agcgggcgct ctccatgggg gtatcggagg cgacggagcg cgtcgccctg atagtcatgg
     2101 gcacccctcc ggatatctcc gatatcctgg agctcgacgg cgaagggccc cgctggagcg
     2161 aggcggccgt cagggaggcc ttcgatatcg gggacctgga gatcgaggcc gcgggggagg
     2221 agaggatccc cgacctcgtc ctggaacggg tggccctaat agacgcttac cgatgagagg
     2281 atcttatgaa agaagagttc tacacccgag gccgatggca gaactggatc aacaagataa
     2341 aggagagcgg gttcaccctc caggaatcgg aggaggaccc gtcggcggcg gtcttcgtct
     2401 acgccatgga cgatgtggtc ctggcctgcc tcaaggtcat agcgcgatgc gaacacggca
     2461 ccataactaa agaggaggcg atctccacca tcgacgagat cagggagatc gtctccgaga
     2521 gggacgactc cctgggggag gacgcgaccc tgatgctgga gtccctgaac gcctccctct
     2581 cggcggtctt cctggcgagc cagaggtaca tcgatggcag ctacgaccag aagacccccc
     2641 tggcggatat ggtgaggaag gcagccaccg tcgaggaggc ggggaggatg gaggaggccc
     2701 tggggatcct gagcgacatc ggggcgaggg tgatcggagg ggaggcggtc cccgaggagg
     2761 cctttgcaga gcttccctac tgcatgacgg cggagctcct ggacggcatc gacgccatct
     2821 ccgccgccca ggtcggggac gacagctaca aggaggatga cggctccgag gacgacggcg
     2881 aggacgcctg accggacctt caccccggcc tcgtcgtcac ctggcggccg ctatccagga
     2941 cgatcaccgt ctcctcggcc tgggagacga gggcgccctg cgcctcccag aggagggggt
     3001 agcggtggag gacccccgcc ctgaggagct gatggaggga gtaatcgatc ctctcccccg
     3061 atagccaacg ccgggcgaag gggagggagg ggtaactatc caggacctct tttaagagag
     3121 ccctcgccgg cggggacctg atcggcctct tcccgatgag ggagtatatc tcgacctggg
     3181 gcgcctccga tatccggccg atgccgtcgg tggcaaaggg ctcgatagcg atgacgtccc
     3241 ccggagatag ctccgccccc ttctcggcgg cccggttggg gatcgagggc tccccgtggg
     3301 cccggtagcg tccgaggccg tggcccgtca ggttcgcgac ggagctgtag ccgtaccccc
     3361 ggatcgcctc ctcgatggcc gcccccaggt cggcggtgga cgctccgggc tccaccagct
     3421 cgatcgccgc ggcaaggccg gccttcgagg cctcgacgag gtcggggtgg cccccgaggt
     3481 ccaccgtcac ggcggcgtcg gcgatgtagc cgtcgacgtg gaccccgatg tccagcttca
     3541 ccatctcctc gccgaagacc gccccgtccg ccggccgggg ggtgtcgtgg gccgcaaccc
     3601 tgtccctgga gatgttgcag gggaaggcgg gctcgccgcc gagggccagg gtcttcccct
     3661 ctacgaactc cgccacctcc aggatggaga ctccgacgtc gatcatcgag gccgcctcct
     3721 ccctaacccg ggccaggatc tctcccgccg tcctgtactt ttccagcact tcagcgtcca
     3781 tataaccata ctcctatcgt caaaacgccc tcgtcagagg atcagccgct tttcgaagtt
     3841 ggtgaggttc tcggaccctc cccccctcac caccagcaga tcctcgagcc gaaccccccc
     3901 gagctccggg tagtagaggc ctggctccac ggtgacgacg ctctccgccc agagggcctt
     3961 ccccacctcg gagagggagg gcctctcgtg gaccgcaagc cccaccccgt ggcccgaaga
     4021 gtggatgaag ccccgaccct cccgctccgg gtagccccgc tcctcgaagg cctcgcagac
     4081 cttcgaatgg accatccttc cgctcgcccc cgccctgacg gccgcgatcc ccgccacctg
     4141 agccgccagc accgcctcgt agatctcgac cacctcgggg tctgcctcgc cccggaggac
     4201 ggtccgcgtc atgtcagcga agtatcgcgt ccgtttggag cgggggaaga tgtcgatgac
     4261 gatgggggcg tccctcggca gggggccaga gccgacgagg tgggggtcag ccgcctccgg
     4321 ccctccagcc acgatcgtac cttccgcctc acacccccac tccaggagag agacctcgat
     4381 ggcggacctg acccactcgg aggtgagggg cctcccctcc aaaaagaggg actctccggc
     4441 gggccgggag cggcggatca tctcgatggc gaccttcatc gcctcctcgc aggccctctg
     4501 agaatatctg atcgcctcga tctcctcggc ggccttcacc tctcgcatct ccaagacggg
     4561 gctctccagg acggatatct cgaactcccc ggcgaggtcc agatagatcc tcgcgggaaa
     4621 gtctccgaag accccgagcc tcttgacgga ccggtcccgg agaaactcct taaggaccgc
     4681 gacgtaggca tcctcaggcc gtccgaacct ggccatcttc tccaagatcc cgtaatcgga
     4741 ggtgcagacc acctcgtcgg cgaccgactc cttcctggcc cggcccgcct ccatccccga
     4801 gacgaggatg gatagcgacg gcctggccag cagggcgaac ctgtcggggg ctgaaaagcg
     4861 ggataggtag tagatgtcca ggtggcagcg gccgtctccc aggaggagaa agccgtccag
     4921 ctcgtgatcg gagagaaact tccccgtctg aggagacgaa gggggcatgg gaggggatca
     4981 ggggagatag tataaaagga tagccataaa agggaggccc gcggcctgag gcgagctgat
     5041 cagaggccct tcatcgccga agtgatctcg cggacgaagg cctcgacgtc ctcggacctc
     5101 tcgaccccgg tcccggtgac gatgatctcg gcccccgcct ccaccaggcg gcgggccgca
     5161 tccccggacc ggatccctcc gccggcgatg agcagggcgt cgccgatggc cctcttcgtc
     5221 gccgccacca tcttcggggg caccggatcc ccggcccccg accccgcctc cagatagacc
     5281 atcctcatgc cgaagtactt cccggcgagg gcgtaggccg cggccagctc gggcttatcc
     5341 ctcgggatca acttcgcgtc cccaacccag cccaccgtcc ctccgggctc gacgatgatg
     5401 taggccatgg ggatcggctc gaggccgtac ttatagatca acggcgcccc gagggcctgg
     5461 ttctcgatca ggtagctcgc gctcctggag ttgaggaggc tcatgaagaa gacggcgtcg
     5521 gcgttggaac agagccccga gacgccgctc gggaagagga tcaccggcag gccggactcc
     5581 gccctgatcg acctcacagt atcctcgagg gcgcggcctg ccgcgccgac agaccccccg
     5641 accatgatgg cgtcggtgcc cccctttgcc gccgccttag ccatcctgcc ggcggcctcg
     5701 gggccctggg agtcggggtc gatgagggtg aggtgggcag cccctgccgt ctccacggcc
     5761 gtcgccagca tcccctctac gggaccgatc atgaggacct cagcccccgg cccggctacc
     5821 ctttgctctc cttggacttg acgcgaagcc ccttgtagcc gcacttgcgg catcgggttg
     5881 anntgcagcg catgcagatc ttcatgttca gaagtcggtt ttcagcctct ggaaatcgag
     5941 ccatggaagt atcctctctg atctttatag cggttcgtca tccctgacga cggatatcgc
     6001 cagacggacc gcggcgatat gaaccttttg gagggtttca ggcgccgggg ttgggatttt
     6061 cctgg
//
LOCUS       DASM01000110           11781 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_70781, whole genome shotgun sequence.
ACCESSION   DASM01000110 DASM01000000
VERSION     DASM01000110.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11781)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11781)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11781
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_70781"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2444 a         3543 c         3685 g         2109 t
ORIGIN      
        1 gatttcatca gggtagatta caccgagagc gtcgacggcc gggtgatctc caccacggac
       61 cgggacctgg ctatgaaaaa cgacatctac gaggaggggg tcgagtacgg ccccgccctc
      121 atcatcgtcg ggtcagggga cgtggtggcg gggttcaaag aggagctgat cggaaaggag
      181 ataggcgcct ccggtagcgt ggaggtcccc ccggagaagg cctacggggt ccgcgacccc
      241 gaggacgtcg agctgatccc cctcactaag ttcaagaagg agaggcctga gatcgggatg
      301 cgggtctcgg tggacgggaa gatggggacc ctcacccggg tcatcgggag gaaggcgcgg
      361 atcgactaca accacgtcct cgccgacaag gtcgtcaagt atgactacac catcgtcgaa
      421 cggatcgagg ataggctgga gaagctggag ggcctgatca ggatctttgc caagatagac
      481 ctcaaggccc agatcctgga gggggacata gccgagatcc agtccccctg ggagatgagc
      541 tactatcggg agtggccgat gatgaggagg gggatcgccg agatggcgat gcggctcctc
      601 gacctcaaag aggtccgcta cctggagagc cacaaggtcg ccccgaccgt aacctcagag
      661 ctcgtctcac ccccggagaa ggccggtgag gcggtggagg ggtccagccc ctcggcaggg
      721 gagacctcag aatagacgcc cctaagagat gagcccccct gggctggagc cagggggtgc
      781 acatctcgtc cccggcgtca gagctcgccc cgccggatga gggtcccaag cccagtccgg
      841 caaaaggttt tatacggcct ggggtctacc ttatcgccat gacgcaagag caatctgcgt
      901 gggatcgttt tctataacat cgaagcgctc cggggagaag aagttcgaat tttcgacacc
      961 accctccggg atggtgagca gacccccggc gtctccctga ccccggagga gaagatcacc
     1021 atcgcccggc agctcgacaa gctcggcgtc aacgtcatcg aggccgggtt tcccgtatcc
     1081 tcccgggggg attttgaggc ggtgaggatg gtggcggccg aggggctctc tgccgaggtc
     1141 tgcggcctcg cccggatcat gagaaaggac atcgacgccg ccctcgacgc cgaggtcgac
     1201 atggtccacg tcttcgtctc cacctcagat atccagatcc agcacaccat caagaagagc
     1261 cgggaggaga tcgtcgccca ggcggtggag gcagtggagt acgtcaagag ccacgggagg
     1321 acctgcctct tctcggcgat ggacgccacc aggacccccg tccccttcct gaaggagatc
     1381 ttcggggcgg tggagggggc gcgctgcgac atcatcaaca tccccgacac cgtcggggtg
     1441 gctatcccta cgaccttccg ccagctggtg agggagatct gtaactccac gaagaagatg
     1501 gtcatcgacg tccactgcca caacgacttc ggcctcgccg tcgccaacaa cctcgccgcc
     1561 ttcgaggcgg gggcgaggga agtccaggtg gcggcgaacg gcctcgggga gcgggcgggg
     1621 aacgcgaacc tcgccgagac ggtgatgggc ctccattcga tgtacggagc taagaccacc
     1681 atccacaccc cctacctttt cgagacggcg aagctcgtag agcggctcac ggaggtgagg
     1741 atatccagga ccgctcccat cgtaggcgag aacgccttcg cccacgagag cgggatccac
     1801 agccacgggg tcctggagag gtccgacacc ttcgagccgg ggatcatgac cccggagatg
     1861 gtcggccaca agaggaggat cgtcctcggg aagcatacgg gacggcacgc cgtcaagcag
     1921 tccctctcgg agatggggta tgcccccaac gacgatcagc tccaggagat cctggagagg
     1981 gtcaaggacc tgggggacaa ggggaaggcg gtcaccggcg ccgacctgat gacgatcgcc
     2041 gaggtggtga tcggcgagct ggcgaaggat aagcaggcgg tgatcctcaa ggagctggcg
     2101 gtgatgacag gaaacaccct cacctccacc gccaccgtcc gggccgtcgt ccggggggag
     2161 gagcgggtcc tcgccgacat cggggtcggc cccgtggacg ccgccctcaa cgccgtccgg
     2221 gggatcctgg gggaggagac cgccgtcaag atcagcgact tccggatcga ggcgatcacc
     2281 ggaggctccg acgccctcgc ggaggtggtg atcggggtcg agaacggccg cctcggaagg
     2341 aaggtgacgg ccaggtccgc ccgggaggat atagtgatgg cctcggtgga ggcgctgatc
     2401 aacgccataa acaggctgat gcttctggag gaagacgcga tttgagcgac acaaaattcg
     2461 aggtgataga cacccactgc cacctcgact tcaagaactt caaccgggat cgggacgagg
     2521 tgatccagag ggggcgaaag gccggggtga cgacgatcat caactccggc atcgacctca
     2581 agaccaacag gaagagcctg gagctggcca ggaggtacga cttcgtcttt gcgaccctcg
     2641 gcctcaaccc caacggcctc ggcgagacga ccgacgagga ggtggaggcg accctcgacc
     2701 agatccgggc ccatgcaaag gagatcgtcg gcgtcggcga ggcggggctc gactactacc
     2761 gctgccccga cgagaggggg agggaccggc agcgggaggt ctttcggaag gtcgtcggcc
     2821 tcgcggagga gctggacctc ccccaggtga ttcacgcccg cctcgccgag gatgaggcct
     2881 tcgatatggt caaagacctc aacaaggtcg tcttccactg ctacagcggg acggtcggga
     2941 cgatgaagga ggccctcgac cggggcttct acatatcact cgccaccgtc gtctgcaggt
     3001 ccgcccagca ccaggttctg gcgcggcagg tccccctgga ccggctcctg atcgagaccg
     3061 acagcccctt cctctccccc cggaagggga ggaacgagcc cgcctacgtc ctcgacggcc
     3121 tccacctcat cgccaggatc aagggcctcc cgcccgaaga ggtggcggag gcgacgacca
     3181 aaaacgctcg aaagatattc cgcctctgag ggggatcgtc gggacggagc cgccttcctg
     3241 catatggccc ggctgccgtc ataccgaccc ctccaggcat taaccccttc ccgcctatta
     3301 acggaaggcc tccgaattcg gcgaacccct tctccgcccc gccatcagca acgtcccatt
     3361 gccaccatct atttgtatca cctctcggag atgagaggct aaaggttgga ggatctggtg
     3421 aggtgagatg aaggcgaagg atctcttcgg gggcttcaga accgagaggc tctcccttat
     3481 gggcagaggg cagagggatc ggggaagcag ggaaggctgg gacgaccccg acccctggga
     3541 cgacgatcgg tactctcctt ccggtgggtt tcggcttccg gtgacctact cggggatgat
     3601 tctgatctta tgcgtcctct tctactttgt gagcgtcgcc tacccctctt tcacattcaa
     3661 ctatctggca ttgagacccg ccctcctcgc cgcccatccc tggaccctca tcacccacat
     3721 cttcgtccac gccgatatgg ggcacctcct cttcaacatg atcttcctct tcttcttcgg
     3781 gaccgagctg gagcggaggg tgggcgagtc gaggttcctc cagatcttca tcatatcggg
     3841 gcttgtcgca gccatcgggc agatggcggt gctgcctgcg gggagcatgg tgggcgccag
     3901 cggagccctc ttcggcgtca tgggctgcct cgccgtcata gcccccgaga tctcggtctt
     3961 actattcttc gtgatccccc tgagcatcag ggcggcggtg gtcctctttg cgatcatcga
     4021 cttcgccttc atggggtctg ccgacaacat agcccacatg gctcacatag caggcctcct
     4081 cgtgggcctc gcctacggcg gtatcttgaa ggatcgatat cgtcgaagat accgctgagg
     4141 tcagcaggaa ccgttaagct cttgtggggt gggcgaaaga gaagctccgg ggcgacggag
     4201 agtcaagtgg tgactgatcc tcgcccctac ggaacgtggt ggtagtctgg gagacgagat
     4261 cgggatcatc gtgggggaga cgaccccctt cgagttcaag tttctgatgg cgggagaggc
     4321 ccccagaggc agctacgtaa aggtcaagga gggatcgaag gactggatgg tcgcccaggt
     4381 gttggaggtg accaggtcca acgtcgccta cagcctcagc gccatcgacg aggagaaccc
     4441 cggggctaag gagagggtcc tggggctagc ccgggtgatc ggggtcgcct cgggaggcag
     4501 gctggagatg gcggccgccc ctccccgacc gggagatccc gtattcaggg ccgacgacca
     4561 tctcatcgag atggctctgg gcctctcgaa ggggaacctc tacatcggcc tgttggaggg
     4621 gcacgacctg aaggtgaagc tcgacgccga tgccctcgtc cagaagcact gcagcatcct
     4681 cgccaagacc gggagcggta agtcctacac cgccgccgtc gtcctggagg agctcctgga
     4741 gaagggggta gccctcctga tcatcgaccc ccacggcgag tacgcctcga tgaagcagaa
     4801 gaactccaaa ggggagttcg ccaggttcga cgtcagtccg agggggtacg aagtgacggt
     4861 ctactcccca gcgagctccg cggctgcccc ccactccgac cgccccttcc ggttcaacgg
     4921 cagaaacctc accgcccggg agatatcggc gatgctcccc gaggacgtca ccaacagcca
     4981 gcggggggtc ctctacgagg cgataaaggt cctcgagacg gaagggggcg actacgggct
     5041 ggaggagatc atcgcccagg cggagaagga taacagcaaa gcgaagtggg ggctgatcgg
     5101 ccacctcgag gccctggagg acctcggcat attctcaggt aagccgacgg gcctcggcga
     5161 cctcctccag cgggggcggg cctccatcat cgacatgacc ggcgttcctc cagacctcca
     5221 ggggatgatc gtcgcaaagc tcctctccga cctattcgag gcgaggaaga gggggcgggt
     5281 caaccccggg atggtcgtcg tcgaggaggc tcacaactac atcccggaga gggggagcgg
     5341 ccgcgccgcc agcacccccg tcatcaggac catagccgcc gaggggagga agttcggcct
     5401 cggcctcctc gtcatcagcc agaggccggc tagggtcgat aaaaacgtca tatcccagtg
     5461 caacacccag atcatcctcc gggtcacaaa ccccaacgac ctccgggccc tcagcaaggg
     5521 gatcgagggg atgagctccg agctcgaaga ggagatcaag cggcttcccc cggggacggc
     5581 gatgctcgta ggaggcgata tcgagcaccc cgtcaccgtc aaggtgaggc cccgaaagag
     5641 caggcacggc ggggtgagca cccccatcgt cggggatggg agggccgata ggggaggaaa
     5701 gtccgccccc ccccagccga aggggccgga taagagacag gattccggca aggccgagag
     5761 gaaggagccc gccgggggcc tcctccggaa gatcctgggg aaatgatcca ttttatcctg
     5821 acgccgtcag gagatcgccg aaggagacgg aatccgcatg atgaggatcg aggccgcgac
     5881 cgccgccgcc gccccgaaga ggaaggtcgc ctctgggcct atcccctgcc agagggctcc
     5941 tgccaccagc ccagaggcga tggcggcgac gccgacggcg gcgtagtagg ccccgaggcc
     6001 cgtacccctc gcctccgccc cgcagaggtc cgagacgaag gcccgctccg caccgtcgac
     6061 gagggcgaag acgaggccgt agaggacgaa taggaggatg agggccgggg ccgaggagac
     6121 gagggcgaag ccgagggagg tcacggcgaa gaggaggtag ccgagggtca ggaccttctt
     6181 tcttccgacc cggtccgacc acgacccgct ggggatcgag aagatggtgt agaccaggtt
     6241 gaagaggatg taaagaagga taggggcgcc gatggccaga tctccggaga agaacccctg
     6301 ggccctcagg atgaagaaca tgtagctaca gttccccagg gcgaagaggg atgccactcc
     6361 gacgaagggg acgatctccg gggagaggcg ggggcggagg cagcctccgg accgcctcct
     6421 cccctctcgg acggggacga gggggaggag ggcgaggaag gcgacggccc cggcgacgag
     6481 gaagatggtc ctgaagtcca tcccagcctg ccataggagg taggcgaggg ccgacccgac
     6541 gacggcgccg aaggagtcga gggcccggtg gacgccgaag cccctacccc tcccctccct
     6601 ggacgccgac tcggcgatga tggcgtcccg gggggcggac cggatgccct tgccggaccg
     6661 ctccaggacc ttgaggacga agacctgctg ccaggtggcg gagagggcga ggaggagctt
     6721 tcccgccgcc gagagggagt agcccgccgc caccaggggc tttcgcgccc ccatcctgtc
     6781 ggaccaatat ccggagagga gcttgaagag gcttggaagg ccgtcggcaa gccctccgat
     6841 gaggccgatg gcgaggcccc cggcgcccaa ggaggcgatg aagaggggca gaatcggctg
     6901 gatgatctcg ctgctggcgt cgtttaagag gctcgccagg ccgaggaggt atatgttcct
     6961 cttcccaaca tctctatcct ctgggggagg ctctgtcaca gagggctgtt gaagcgggtt
     7021 agggcaagaa aggtttcggt tcgatcttgc tcctccttcg aagattgttt ataggacgga
     7081 ggtcaatgtg gtgtcttgga ggaccgcagc cgaattctcc gttaagaggc agagatgtat
     7141 ctacagtctc cgaggtgaga ggagtggcgg aggggacaag ccccagttct agggagttta
     7201 aaggcacggg gagcagccat agctccccca gatccgagcc ccgagataaa ggcctcatct
     7261 ctgcggctga ggaagatcgc cgcctccgag aggagctctc caagctcctg accgatagga
     7321 gagaggcggg gctagagggg cttgtcggag ggcttgaggc cgtcgtcatc aacaccgaac
     7381 cccacctatg gggggccgcc tccgaggagc ttttgaggtc cacgggccac ggcttcgtcg
     7441 aggcgttcca ggatcctcaa tcgatgacag ccgtcctgag caatgagggg tactcagaca
     7501 ttctagtcag atccaggctc ggtggagata acccatttct gcgtcataac ctgcatccga
     7561 agtccggcca ccttcccaac acgaggcttg agaccctggt cttcaggacc aaagatattg
     7621 aggagctcat ctccatccag aggtccaggg gggtttcgtt cctatccgag gatatgatcg
     7681 gaggcgataa ttgcgccttc atccagactg tcccttccaa atatacagga aactccatcg
     7741 gattcatcga acggggcgga ggagcacccg actatagaac tgccgatagc gaacccctcg
     7801 aatatgatct cgcaaagccg gatctcggcc atctatcgaa gataacctat ctcgaccatg
     7861 ctgcaacccg ggtcagagcc gaggataggg tggctgcgat gaaggagttt ttgagcctga
     7921 caaactactg ctttgatttt gcaatttatg tcagagaatt gaactccatc accagcgtcg
     7981 ccaggttcga aggctcaaaa ttcgccatgg tcttcacctc ggggatctcc ccctacgtca
     8041 gcgaggagat ctcggggccg acggagaagt tcgttcacaa ctacggagcc cgggtccacc
     8101 acctcgcctt ccagaccgag gagatagagg agacttgctc ttccctcatc ggggacggga
     8161 tcgaatttct gatcgcccta gtcggctcgc cggaggaagg tctgaagcag accttcaccc
     8221 agccctcccc tcatactctt atcgtcaacg agtacatcca ccggtacgga gacttcgacg
     8281 gcttctttac aaaaagcaac gtaaccctcc tcaccaaagc cacaggcaat cagtaggaaa
     8341 agggataaaa ggatcggagg tgaagaggag ataaacctgc gggagttaga agatatgaga
     8401 ataagagatc ccctcaagct ggcagcctcc ctgggaggat gttatctggc ggcggccatc
     8461 ggctcgctct tcaccacccc gaacatcccc acctggtacg ccaccctatc gaggcccgac
     8521 ttcgcgcccc ccgactgggt cttcggcccc gtctggaccg ccctctacac catgatgggg
     8581 atctcagcct acctcgtctg gcgggagggg ctcgccgatc ccggggtaag agcagccatg
     8641 gcggtattcg tcctccagct ggccatcaac atctcctggt cggcagcctt cttcggcctc
     8701 cgctcacccc ttgcggggct cgccgtcatc ctcatcctct ggggccttat agccctcaac
     8761 atccagaggt tcagggggat atccaggacc gcaggggccc tcctcattcc gtaccttctt
     8821 tgggtgagct ttgcggcggt cctcaacttt gagatatgga ggctcaattg agggaacggg
     8881 ggctatctct tcaggcagag cccacgatga tcatcccgaa gaggatgttc agggccatca
     8941 ggagaagggc ggccctgccc agaaatctgt ggacccgcct catccttctt ccgagatccg
     9001 tccccaccct cagctggata aatccgagga gggggttaac gatcagaagg aggacgatga
     9061 aggaccccag atgggcgtgc gggaggcgca agtgctcctg agaggcggca gagaccatat
     9121 aagctgcaac agccatcgca gatacggcca aggccgcccc cgagactccc aggatcctgt
     9181 gcttcttcag ccaccccggg cccttccgcc gggcgacggt catcccccag acgaggagga
     9241 ggaacgcgag gacggcgagg gaggcatgat acggccagag cctcgcgatc tcgctggata
     9301 ggccctgatt ggggctggtg gctagggctc cggcggggac ggtcaacaga aggatcgatg
     9361 gagggaaggg ccgaaggtta gagctgatcc gccgacggat cttgcggcct tctccccccg
     9421 gggctggatt ctggttcagg actgagcctc ccttccattc aggaccgaaa gatgagtttc
     9481 gcggcagagg gttcatttca gcttcttgtg acagaaccac cagtcctcgc agacgatatc
     9541 tccccccgtc tttctggtgg ccgcggtctt ctcgtcggag ggcggaggta ttatgacctc
     9601 gtctccgaag aggccattat tcggccagtt ggcggggagg gccaccttgt tcttgtcgga
     9661 gatctggagg gccctgacag ctctgaggat ctcgtccatg ttcctgccga tctcctgggg
     9721 gtagtagagc atcagcctga tcaggccgtt ggggtccacc acgaagacgg ctcttacggt
     9781 gttggtcccc ttgccgggat ggaccatccc gagctttccg gcgacggtgc cgttatccgc
     9841 aatgatggga aaggttatct ttacgtccat tttatcctgg atccactctg tccacttcat
     9901 gtgagagtga acctgatcca tggagaggcc caccagggcg cagttcagat catcgaactc
     9961 cttcttcatc ttctggaagg ctatgaactc cgtggtgcag acgggagtga agtctccggg
    10021 gtggctgaag aggataaacc actttcccga aaggtcggcc gggatcttca tcctgccgtg
    10081 ggtcgtctgg acctccatat ccggtaccct ctcccccagg agggggatcc cagctctcgg
    10141 ttcgtgctcc ataacttttt tcctcctaag attaaaatgc gtaataagct actttaattt
    10201 ttaggccaaa aaaatattgc ccccaatcta tacttgattt ttaataaaaa atgaagggct
    10261 aggagaagga agagggggag ctcagcgctc cgcctcggcc tcgatctcca tgatcagcct
    10321 ctctccaccg ctgacgtaag tcatgtagtg gatgactata cataagcccc agccgatggg
    10381 agagtaccag ggctgccata tcggcaggtt gggattggcc ggcattacca tcatatagtt
    10441 gatcgccaac cacacgatgt tgaccaccag gtagattatg aggtgcgtca tgaacagctt
    10501 cttcatcctc gcctgcatgg cggtcctgaa cgactttttg tacgcttcgt cgaccatctt
    10561 ctaacctcac ctccagatca tgtgctagta tctttctctc ctagttaata tataaaggtt
    10621 tcgcggggga ttgtgctatg attatatgaa aaggtttaca tttgagcgtt aaccgtctcc
    10681 atagagcatt cgtttatgat aattcgcgga gattgaatgc ccaatcgtcc ttgcgagatc
    10741 cccggcgatc tatcaggaaa aaaattgacg aagggaggga agaggcgaaa tcaggccttc
    10801 atcacatctt cgaactccct ctcgctgagg aactgagggg catacatgat ccccatatcc
    10861 tccaggtccc tgacgtaaga ccggaggtgt ttcctcgatc ctgccagaag cccttggaag
    10921 acgaccctca cgtcctcatt ctccgccacc tccagctcct tctccaggtc catgatgctg
    10981 atctcctcga aggcggctgc caccctgagg gcatcctcct ccgatctcat cccccgggcg
    11041 agaagttcct cgtagaggtc gatgagggtc tggttgctga aggcccccgg ctcatcgccg
    11101 acggggagga ccagctggta cttatcgacg agagtcttgg cctggtccat gtggctctgc
    11161 tcggaccttt caaggtcgag aaatacctcc agcttcttag ccctgtagag ctcagcgtaa
    11221 aggtcccggg ccaccttctc ctcctcccat atgaagagga ccccctccgc ctccctgggg
    11281 gttagggtgg ctactgctga tccggggacc tggtcctggg ccaggggaag gatcgcctcg
    11341 ccgtaggcga cgttatgctt caggttcctc gaatccagat ctgccatcgc ccagatgacg
    11401 gagacgctct cgcccggctc gaggagcttg tcgaactcat cgccagtatc gagcttcctc
    11461 ttgaactcta tgacggtata tccgtcctcc tcagacccgg caaactccag gatgtcgtag
    11521 gttcctccca ggaaggtatc gtcttcgtgg gggccgtaac ggccggtgga gtagagatcc
    11581 agaaccgtgg tcttcccgcc gtccaccgaa cccatgatca tatcggcatc cttcatccat
    11641 tccaggggat cgaagcctat cgagatccat ccctcagtcc tccccttcag ggccatgtag
    11701 agatgctcct gatcgttcct ccaggagacc tccagatcgc ctcccgtata gccggatctc
    11761 gcagggccca tcaagaggat c
//
LOCUS       DASM01000111            4431 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_116827, whole genome shotgun sequence.
ACCESSION   DASM01000111 DASM01000000
VERSION     DASM01000111.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_116827"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          833 a         1331 c         1445 g          822 t
ORIGIN      
        1 cgcagagctt ctcgatccac ttctggtcgt aggtgccggt ctggtagctc acaggcatgt
       61 actcaaactc ggcctccttg ttgaagttga tgtagtcgac gcagcagcaa ccgtcattat
      121 agccctcgat gatgcagggg tcagatggat ctgatccata tggagatcca cactcggtcc
      181 cgcagcacca gggtccatag gggtccctct cggcctcgat ctcggtcctc tcggtgatga
      241 cgtttccgct tccagactcc ttctcgacca gcttgtttcc gttgaggccg ggctgagtct
      301 gaatgatgat ctctacgttc ttgtagccaa cgcccttgac gttggccttc tcgtacatgt
      361 agttggcagc gcaggacatc cccaccaggg cgatgcaggc taccaacaaa atagctagtt
      421 ctctcctcgt tgttctccca cctatactgt tgatcggtaa tttgctggcc tctcctcccg
      481 gatcttgccg gcagagcggc ccgctcagat ctcacccacc gaaacgggtt ctgtcgtctc
      541 agcggagtga catggaacct gctttcgcat tcaagtagtt atactttttc gtccagccgt
      601 tgggaaacgt caatagaata ttaacttcat catggcctac tcgtcggcgg gaacgccatc
      661 cggcggatct actaggggcg gaggctctca agagtggtgg cgacgcggcc cttaggcgcg
      721 gcagcctcca tgaaaggggc tgcccggggc cggaaagatg ctccggccgg gatttgaacc
      781 cgggtcttcg gctcgaaagg ccggaatgat tggccggact acactaccgg agcgctcgat
      841 cttcgggtcc gatccaccct gaggcggccg ggtgcccatc ttccagactt tggccctcta
      901 tatctacgtt tctcatctcc gacccgatag gctgcggggg ccccgagggc ggaattttat
      961 agtcccgggc ggattcgaac caccgtccca ggctccaaag gcctgcagga ttgaccacta
     1021 ccccacggga ctcctcggcc gccctcgggc ttgcagcccc cgcggggaga tctatctgca
     1081 taagatatat atccgcatcc ccgccaggga tccggagatg aagctgatca ccaagcccca
     1141 ttctagccag cggaggctga tagagatcct gagggtgata aacgcggcgg agaagccgat
     1201 aggcgcgagg gccatctccg acgagctatc cagcaggggc tacgaggtcg gggagcgggc
     1261 cgtccggtac aacctgaaga tcatggatga gctggggttc acgaagaagg agggttacag
     1321 cggccgcgtc ctcacccccc tggggcgccg ggagctctcc gatgccctcg tcgacgacag
     1381 ggtcggcttc gtcaacgcca ggatcgagga gtacatgtac cggtcgagct tcgatcccgg
     1441 ctctgaggac aacagggtgg tcgtcaacac cagcctcctg gataagaggg actcggagag
     1501 ggccctggca gcgatgaagg cggtcttcga tgcgggcttt gccgtcagca ggagggttct
     1561 gatccaggac gagggggagg agatgggggg ggcggcggtc ccccgaggtt tcctggggat
     1621 ctcgaccgtc tgcagcatca ccgtcgacgg gatgctcctc cggggaggga tcccggtgaa
     1681 taccaccttt gcgggggtgg tggatgtgag ggagggttcc ctctaccagt tcaccgacct
     1741 cctcgcctat gccgggtcgt ccctcgatcc catcagggtt ctgatgtcca ggaaggccgc
     1801 ccggatcacc ggggcggtca agacagggaa cggcggcctt ttggccaacc tgagggagat
     1861 cccggtcgcc gccatcggac aggcccgaga gatgctggag agggcgaggg gggtcgggat
     1921 cgacggggtg atgaggatca gcgagccggg ggagccgaat atggggtgtc ccgtaggggc
     1981 cgggaaggtg gggatcgcct tatgcgccgg ggtcaacgcc gtcctggcgg cggaggagct
     2041 gggggctgag atcaggacga cgcccatctc ctccatccag gagtacggca ggatgaaaga
     2101 gctccgctga ggctgaccac agggtataag atctgcggtg acgaggggtc tcttgtgggg
     2161 attttgatac gcgaagcccg aattctgatg gctccgctcc tgatctcggc ggccctcgtc
     2221 gccactttta tggccgtcgc cacaggcgac ccgtccgaaa gctccgacgt agtccacctt
     2281 ggatcaggcc cccgtcccat ctacaggatc ggatactctg gggatggctc cgtcggcatc
     2341 gacgagctga tcgagcaggc ccatatcagc gtcgcgaagg gggcgggcga tacacccccc
     2401 tcaggcctct cctcagattc gttgtcactt tcgccgtcct cacctctgga ggctccggag
     2461 ttcgaccctc catcagaggt gggcggagca agtccgccgc agctcgcatc cgacggagga
     2521 tctggcgcca ccacctcctc agctgcggcc aggatcggcg atgagaagcc cccctatgtg
     2581 agggttcccg tcgtctccga cccctccggt gcggaggtct acatcgacgg gatctatagc
     2641 ggcgtcaccc ccttcatcgc gacggtggag ccggagagcc acctggtgga ggcctaccgc
     2701 gaaggctatt ggaactggtt caacgtcatc gagttcaccc cctctacggg gactgttccc
     2761 atcaccctca ccccccagga gccggcgtcc aatgggacgg aggaggtgga ggcggcccaa
     2821 aacctctcgg ccctggaggc ggcaaacccc gcggcagggg agaacggctc cctggagttg
     2881 aggccgctgc tccccgatga ggcggacttt ctggaggcga actacaggaa gctcctgata
     2941 gtcgtggccc tcctctcctt cctgatcgga tcggggagca tagccagctg ggcgaggtcg
     3001 aggctcacca cctcaatcag gattagcttc ccccgcgacg gcgaggaccg gtgggcaggc
     3061 ctggtggagg gtcaggcgag ccggatccgc ggctccaaga ggcaggtcta cgtcctggtc
     3121 aggtacgacg acgagcacct ccaggtgagg aggagggtgg agccgaaccg cgacggtcgc
     3181 tggtccaccc ggtgcgacgt cgaggagggg acggtctaca ggatatacgc ggtggtggtg
     3241 gacaggcccc tccccgaggg ggcttacctg gaggagatcc cgccccaccg ggccatctcg
     3301 gaggtcgggc cggtgacggg gaaggggaag ccccagaagc cccccgatgg taaaaacccc
     3361 tctccggatt cggaccccgg ataaggtttt atatgtggat ggggcgtagt ggtggcctgt
     3421 tcagttgcga ggagaaaaaa atggacttca gagtcgttat ctgtgatccc cagagcggga
     3481 aagcttacca ggtggaggcg aaggacgccg ccgccaacaa gtttctcggc cgaaaggtcg
     3541 gcgacgtgat cgatggagac gccgtgggga tgcccggcta ttcaatagag atcaccggcg
     3601 gaagcgatcg tgaggggttc cccctccgtc gcgaccttcc cggctccaag aggaggaagg
     3661 tcctgatcgc cggcggcaca ggatacaatc cctcggagaa ggggcggagg aggaggaagt
     3721 ccgtccacgg ccgtgacatc tcggccgacg taggccagat caacgtcaag atcgccgaac
     3781 ggggcgcaaa gccggtggaa gagctcctgg gcgtctcccc ctcggaatga gccgacgagg
     3841 ccctgaaaag atgcctttct tccaaggacc cccggtccag taagctggcg gccgcggggc
     3901 tatcggcatc cttttggggc cggggtaacg gcccaccacg aagctaaaag ctcccattag
     3961 ggcttcagat ggggatgatg gaggacttga ttatggcaga agaggcccat cagccagaac
     4021 ccccgcctaa acctctgcga ggttcgactc atggtcgagg tcgaggcgaa ggaggagggg
     4081 tcgctcctct ccagggcagc tctggagagt tcggttttag tggccatgcc gtcggatctg
     4141 agggtcggcg gcggcttggt tccggccctg ggtaggatct ccaagatcgt aaagcagagc
     4201 ttcttcgccc tggtcatctg ctccgtcacc tccatggtgg caggcttctc cctcgccaag
     4261 atgtcccatc ttttggccct ctaccccggc ctgataattc tgatcccagg agccatagac
     4321 atgcggggga cgatcttcgg gaccttcggg gcgaggctcg ggaccagcct ccacaccggc
     4381 gagatatctc cacccctcgc gaggtccgag cccctccgcc agcaggtggg g
//
LOCUS       DASM01000112            9190 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_162105, whole genome shotgun sequence.
ACCESSION   DASM01000112 DASM01000000
VERSION     DASM01000112.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_162105"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    134..214
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1624 a         2856 c         3070 g         1559 t
ORIGIN      
        1 agctcaccgc ctccgccatc ggccgggagc tcggcatcct gaatgagggc ggccgtgtcc
       61 tggaggggac ggagctggac gagatgaccg ccgaagagct cgccgagatg atcgaggagg
      121 tctcgatctt ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncggata tggtcatcgc cgacgacaac
      241 ttcgccacca tcgtcgccgc ggtcgaggag gggaggagga tctactccaa catcaggaag
      301 gcctcatctt acctcttatc ctgcaacttc gccgaggtga tgactatatt catcggcgtc
      361 atgctggggc tgcccgtacc cctcatcgcc ctccagatcc tctggatcaa catcgtcacc
      421 gacgagtttc cggccatcgg ccttgccgtc gagccggccc actcggacct gatgaagaat
      481 aagcccagag acccgaagga gccgatcctg acccgggggc tcttcctata caccctgggg
      541 atatcgacgg tcatcttcat cgggaccctc ggcctatacg ccaacgccct ccggggcggg
      601 gcccccctcg aggaggcgag gacgacggtc ttcgcatccc tggtgatctt cgagatctac
      661 aacgcctaca acagccgctc cctccatacc tccttcttca agatggaccc gaggacgaac
      721 ggaaagctga tcttggggct tatcgcctcc ctaggagccc tgctggtggc gatataccac
      781 ccctggatgc agaggctctt cgagaccgcc cccctggatg ccggaagctg ggccgccatc
      841 gtcgtcgcgg cctcggcggt ggtggtggtg gcggagatct tcaagcggtg ggagcttcct
      901 gagaggagcg gacgcccacg atgagatccc ccgggcccag gttggggggc acggtggatg
      961 gagaggaaaa gaagccgccg ggttagatag aaacttcagg gaggaggaga aggcgctgag
     1021 cccccggggg agggggtgaa gaggtagaac ctcgcctcgc cggagttgac cagggtccgg
     1081 cgaaatcccg ggatcgaagc gatctcgtct ccaaggggcc ttaccatcac ctcggccccc
     1141 acctttttgg ccagcctccc catggcgagc tggatctcca ggggcggcct tatggagtag
     1201 atcagggacg cgccccggta tagatccaag ctcggggaga atatgtcatc gccgacgacc
     1261 gccatgcccc ccagggcccg gcccacctta tcggtggcga cgacctcccg ctgagggatc
     1321 aggaggaggg cgacctcggg ggcggcgccg atccccacct cgacgacttt gcccgagtaa
     1381 cgggccagga tgaaccgggc gaggtcctcc ccgccccgga tcggtggcat cgaggggccc
     1441 tcagagcccc agcttctcca gggcgatggc cgccgccttc tccccggaga ggagcatccc
     1501 gccgaagatc gggcccatcc gcggaccccc ggcgacggcc gtcgccgcca tccccgccgc
     1561 tatgagccct ggatagacct cctgggtcac ctcgacgacg gtcctctcgc cgacgtcggc
     1621 ccacatcggc ttctccccgg ggacccccag ggacccgacc cgtgctccgg ggatcttcct
     1681 ctggacgacc ttacagatgt tggcgtcgtg gccggtcccg tcgatgacca ccttcgccct
     1741 caccgataag gggtcgacgt ggagcctcgt cctctccacc gcctcccagt tgatgacgag
     1801 gcccgcgacc ctctcgtcct cccggatcat cacgtcctcg acggcgacga ggtttatgat
     1861 ctccgccccg gcgtcgacga cccctgccgt cagcttcgcc accgtctcga tggagcttgc
     1921 tatgtagtag ccttcagcgt actcccggta ccagaccccg aactcgtcca ggatcctgca
     1981 accctccttc tggacgacga tccgggggaa catcatcccg cccccccaga tcccgccgcc
     2041 gacggccagg ttcctctcga agacgacggt cttcgcgtcc gcctcggcga gcttctttgc
     2101 cgccaccagg ttggcggggc ctgcccccac cagggccacg tccacgtcgg tgtacttgag
     2161 gaaggagtcc atgtaggact cgactatcgc cttggtgatc gtaacttcat ccagcgccat
     2221 ggatggggcg atggcccgat tcctgataaa gatatccttt tcggaaattc ttatttaaca
     2281 tcccgcaaac cccatagccg tcggagatcc gccggggcgg gggctgggac ggaaactccc
     2341 ctccccgggg tcttccgggt gggatgatat gaatcgagct gccggaggcg ggatcggggc
     2401 cgatgaggcc ccttactctt acgacggcga ggagttcaga aagcctcgaa tcctggtggt
     2461 cggctgcggc gaggccggat cccgaatggt gaggaggctt gaaaaggtct gcccctcggg
     2521 ggcggacctc ctggaggttt gggacagccg ccgcggcagg ggtccgggag gagggggggg
     2581 ctcggctgag gggataggcc ccagaaccga tgagggaaag gccgccctgg aggaggccct
     2641 ccagggagcc gacctggtct tcgtcctcgg gggcctggga gaggggatga agccggaggg
     2701 ggcggccccc gccgtctgcg agatggcggg ggagctgggg aggacgacgg tggcgatctt
     2761 cacccttcgg ccggaatccc gcgaggacga ggagaggcgc cgccccggcg gagccctgat
     2821 gggagagctg gcggaggagg cagataccgt cctcctcctc gacggcggcc gagttcttga
     2881 ggcagccccg ggcctctcgg cggaggaggc ctcctcggtg atggaccagg tggtggccga
     2941 gatcctccgg gggatcgtcg agacggtcca ggagacctcc ctcataaacc tcgactacgc
     3001 cggcctgaag aacgtcgtcg gaagcggcgg cctcgcctcc gccctggtcg gcgaatcggg
     3061 ttgcgaagac ggtcccgagg cggcggtccg gtcggctctc cgaaatccga tgctggaggc
     3121 ggacctccag agggcgaagg gctgcatcct caccataacg ggggggcggg acctcaccct
     3181 ccggaaggcg gcggcgatcg cctccgccct gaagggggag ctagggccag aggcggagct
     3241 ggtctggggg gcgaggttga aggaggggca cgagggaaag gtcggccttt tggcgacgat
     3301 cgtcggggtg gaactaccga ctgatatccg ggagtccggg accgctcgag gggttatggc
     3361 ggcgaggcgg gactgagtcc gagaagcccc tccgatctat ttggaccctc agaaggtccg
     3421 cccttccggc agcactggcc ttacctttag gaacttctcc tcctccccca gggtcatggt
     3481 ggcgtcgatc cccaccttgg tggtgatccc gtcgttggac cgggggtcga gggtgctccc
     3541 ccggacgttg gggtataaga ccatgtcctc atcccccttc acccgggtgg cgacggcgta
     3601 ctccagctcc tcggggctgt agatatccac gtcctcgtcg acgacgatga catgcttcag
     3661 gctcttgtgg gcctcgaagg cggcgtcgat cacctttccc ggctcggcct cgcccctctt
     3721 cttgatctgg acggcggcgt ggaggtagga gcaccccccc tccgtcagga ggacgttctt
     3781 cacctcggcg acctcggacg ccgccctgaa gatgaggggc tcgtagggga cccccatcag
     3841 catcttgtgc tcgccccccg aggggaggag ggcgtgatag atgggaacct ccctcgtcat
     3901 catcttcgat aggtggatca ccggctccat cctcggaaag tcccgagtcc cggtgatgtc
     3961 gacgaagggc ccctcggagg ccctctcgcc gtcgatgtac ccctcgaagg cgatctcagc
     4021 gtgggggaca aaaacgccgt tatccagggc caccagctcc accgggcttc ctgatagggc
     4081 gctggcgtac tgaaactcca tccccagggg gacccgggtg ctggcggcga tcaggactaa
     4141 ggggtcgacc ccgatgacga tcccgacggg gagcctctcc ccggcggcga gagcctcctg
     4201 gaggagcatg gaggtgtgcc tcccggggac gagccgcgcc gcaactctat cttttccgag
     4261 gaccatcatc ctgtggacgg aggcgttcgt ctttcctctg aaggccgaga cgacgatccc
     4321 cgaggtgatg taggggccgc cgtccccccg aaaatgggtg aggatcggaa gggctgtgag
     4381 gtcgggctcg ccgacggcct ccatgaaggg ggaggagtcc acctcccgga tccagccccc
     4441 cgcaaggtcg atttcggcga gggccttcgc catatcctcc ctggggatcc cgagggccaa
     4501 agccaggagg tcccgggtcc ccaggaggtt catgcagcac ttcttgccgt cgacgttgtt
     4561 gaagaggagg gggcccgccc cgacggagag ggccgccgcc tccagggatg gcgagacggg
     4621 gcggtccacc tcctccaggt ggccggcctc ctggagggcg tggaggaaac ctcggaagct
     4681 catggaggat gagacgccgc ccccccgata aagacctttc cacctcagac gatcaggact
     4741 gcgatcaccc ccgtcaggaa tatcccgtcg aaggtccccg cccccccgat gctcgccacc
     4801 ggggctccca gctccgagat ccgggggagg ttgaggaggt cggcgccgat cagggttccc
     4861 aggaccccgc cgacgtaggc ggtgacgaac cgcccctcag ggatggggga taggacggcc
     4921 gtcaggagga gggcggaagc ggccgccgtg aggggcggta tgaagagggg ggtggcgatc
     4981 cccagccccc ggatcggcct tgcaacggcg tgggtcacca gggctacgat cgccgtcccc
     5041 gccagggcga cggccgccgc cgccgggtaa gatatcaaca ggtagacgga gacgaggacg
     5101 gggaggaggg cgccgccgac gttgacagcc accgtcgtct cgttcttcac cgtcttcacc
     5161 accgggaccc gatagaccat cccgaaggac cggacgaagg cctccctcac cacggggacc
     5221 ttcgactcca gggtgaagag ggggatgttg atcccggctc cgaggaggga ggcggtgagg
     5281 aggaggaggg cgtcggacca ggagaacccc atcctggtga aggcggtgct gagggccccg
     5341 aagaagagga gggcgacgct cgcccagagg agaagggcta aaaggaggat cgccaggatc
     5401 ccgaagggga ggtagatcat ccggtggttc atgagatcga gtcgaccttt ccaggtataa
     5461 cctttcgtcc tcggggaagg aggtggtccc cgtctccggc gggatggccc ccccgggcaa
     5521 aaggctcatc taactcaaga taagagatct cgcatcgata gtccaaaaag gtcttgaagg
     5581 gaagcccagg agctgataag atgaaagaat ctctcgccga tacgatcaag cacaggataa
     5641 tggtgatgag cgggaagggg ggcgtcggga agacgacggt ggccgtcaac ctcgccctgg
     5701 gcctcgccct cagcgggaag gaggtgggga tcctggacgc cgacatcacg ggaccgaacg
     5761 tccccaagat gctgaagatc gaggacgcct tcctgacggg ggagggggag gacgagacga
     5821 tccaccccgc ggagatcccc ataggcctag atagcgggat caaggtcgtc tccatggcct
     5881 tcctgatcgg gagggagagc cccgtcgtct ggagggggcc attgaagatg cagatcctta
     5941 agcagttcat cgagggtgta gcctggggag acctcgacta catgctgatc gacctgcccc
     6001 ccgggacggg ggatgagccc ctatccatcg cccagctcct ccaccccgat gggacggtca
     6061 tcgtcaccac cccccaggac ctcgccctca tcgacgccag gaaggcgata gatatgtcta
     6121 ggtccctggg ggtcccggtc ctggggatcg tcgagaacat gagcggcttc acctgccccc
     6181 ggtgcgggag gtcgatcgac ctcttcaagg tcgggggcgg gatgagggcg gcggaggagc
     6241 tcagggtccc cttcctgggg aggatcggga tcgaccccgc catctgcgat agcggcgacc
     6301 gcggcacccc cttcatcctc gccgtcgcct cgaagaacgc cgaggccttc gacgggatcg
     6361 tgaggatgat catcgcccgg atggaggggt cggaggggga tggggggtga ggggatcgag
     6421 ccgctacgtg gagggatatt gaagcggcga aaaaataagg agggatcggg attcgcctcc
     6481 tgggtggtca ccacttcccc gacttgatga cgttcaggct ctcgatgacc ttcgccgccg
     6541 ccaccgggct gagcttgtgc ttctgacaga cggcgatggc cctcgatatc tctccctgct
     6601 cgtcggagcc agacctctgg gataccatca cctcggcggc ggcaaacccc ttctccagga
     6661 gggccatccg gacctcctcg aggagggcct tgtccatgca gaggggtccg gtcccgatct
     6721 cccggaccac ctcgtccatc ttctccatct ccttcgtctt cagggtctca cctccagacc
     6781 tcatcctaac ttgccgatcg agatccggct gatctcggcc cctatagccc gttcagatcc
     6841 ccatggatcg gggcatcgac gtcttgggac ccccgacccg gcagcttttt tgcctatagc
     6901 ttggtcggga aaacataaaa aattttcgtc ttcgtggcga ggaggaggag gagctttggg
     6961 gaggggatcg ggccggaggc gtggtaagaa gaaaaggggg ctccgggtgg ggaggatgaa
     7021 ctgggatgag cctaggaggc ctgatctccc cggggccctc cctccggcgg ggcgccccac
     7081 ccctctcgga gagtctccgg attcgacagc accaggttgc ggtaggggta gggtatctca
     7141 atcccctcgg cgtcgaaccg cctcttcacc gcctcccgga tctcgcatcc ggtggtgaag
     7201 gcgacatccc ttttcggcag ttccatgctc agcctcaggt tgacggcgaa gtctccgagc
     7261 tctgtcaccc tcacggagat ctctctttct tttaggacga gggggtggcg cttcgccgcg
     7321 tcgatgatga tctcccgcgc ccgatcgata tccgcggtgt agcctatccc gaagtcgacc
     7381 cgccaggtta tgatggggtc gacgatcgac cagttgacga tcggcatatt ccccatgatg
     7441 ctgttgggga cgaagatcct ccggccgtcc caggtcttga tggtggtgtg cctcagggtc
     7501 aggtcctcca cctggcagta ctccccctcg aagtcgacgt agtcgcccac cctgaagggg
     7561 tggaagacgg cgaggaatat ccccgagatt atgttcgaga gggagtcctt ggcggcgaag
     7621 ccgatggcaa ggcccgcaac cccggccccg gcgaggaggg tgacggcgac cctgctgagg
     7681 accggtatct gatatatgac caggatggcg ccgacgatgt agatcgccgc caccagaagc
     7741 cttcgggtga tggtcctgaa ggtcatctcg gtctccatgt ccatcccccc cttggctttc
     7801 ccccccacct tggggatggg ggcggtgagg gcccggtcga cgaccctggc ggcaagccag
     7861 gtgaggacca ggatggccag gatggtcaga agggtcccga ggagggagag gacgtcgatc
     7921 cccagggacg agctgaggtg atcgacgacg gagaagaccg ctgagggggc gccgtccccc
     7981 gtcagctgga cggggatctc cgacgggata ccatcggatc cgttggctcc gctcagggcg
     8041 gagtcgacct cctcggggcc caggtcggcg ggtatggtgt aatcgtagaa gatcccctct
     8101 tccgccgacg tcgtctcgtc ctcgtccatt gagccggaaa gactgcgcca aaggttaatt
     8161 aagggttccg ttggggggag ggaccctcca aaaggatatt gagccttgag ggacgataag
     8221 tagccatggc cgtccacgtc aacaagaggg gggtccgggt cctgggggtg gccgagagct
     8281 ttctgaagac ggagccgagg tctgtcctgg cgggggtggt gatgaggagg gacctgcgga
     8341 tcgacggctt cggcctcgcc gagatcaccg tcggaggaga cgacgccacc gagggggtcc
     8401 tggagataat cgagggtttg gggaggaggg acttaaacct cctcctcctg aacggatcgg
     8461 tggtcagctg gttcaacgtc ctcgatctcg acctcctcca ccaggagacg gaccttccgg
     8521 tgataagcct cacctaccag gagtcggagg ggatcgaggg ttacatccgg gggtacttct
     8581 ccgatcccga ggagaagatc gcccggtacc ggaggctggg gccccggagg gccttcaggc
     8641 tgaagacggg gtacgaggtc tatgccaggt ccctcggggc gacggcggag gagacgacgg
     8701 gcctcctcaa ctccttcacc ctggagggga gggtccccga gcccctccgg gtggcgaggc
     8761 tcctggcgag gaggctcctc aggagaggct ccggtggagg ccatcggccc ccatgaagga
     8821 tagataaaaa ggtcatcggt ccggcggagc cggagtttag ggggcgcgag tctggagagt
     8881 aagccccctt ctgttcgtgc cttccggcat tggcggcatt cagctcagcg ccatacccgg
     8941 gcgttggctg gactccatcg atcgccctta ttttggcttg cacccgcagg gtttcgccgt
     9001 ttcatctgcc cccctcgcga cctcagcctt tcggcatcat ggcgttggcg aggtgcagtc
     9061 tcgtttctgt gccagagcca gagctctcgc cctacaccgg aaagaggtgt ctgcgtgtcc
     9121 agaggtgggg ggactttcca caccggcgaa gggttcgcca gcggcagccg cccttccact
     9181 ctcgcgcccg
//
LOCUS       DASM01000113           12622 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_174329, whole genome shotgun sequence.
ACCESSION   DASM01000113 DASM01000000
VERSION     DASM01000113.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_174329"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2227 a         4194 c         3850 g         2349 t
ORIGIN      
        1 tcgctcttca tcttcacctc ggcccgggtg gcgtactcgt gtttcgacaa aacccggcgg
       61 gcgatctcgc cgcagagctc ctcgatgacg tagaccggct cgttgatcgc ctcctccagc
      121 acctcgtcta tcacctcaaa gttccgggag aggttcgccc ccttccggtc ggaggggagg
      181 tccacgaaga cctcgaagtt cgatatcaga actatgggcc gctcctcgtc ctcccggagg
      241 accttcacca gcttcttcac cccggtgagg ccgaccctgg tgaggtttat ggggtactcg
      301 ggttgacagg cctgaacatc tggcagcgtc tccaacgggg atcaccctca gatatcttcc
      361 tcagactcca tctcttcgga gagggctatg agcccctcca cctcctcgag gagggcgtcc
      421 agggtctcgg atatctgacg atcccgctcc tccagagcct tgaggaaagc ctccagatcg
      481 atggatagct cttcatcgtc cagatcgagg aggtcgccct caagacctct ctcgatcttc
      541 tccaactcca gcatgagagg atcagaggga ccaaattcta tttcagcctt tgcccgcagg
      601 ccgaagatcc ctcggaccgg agggcagaat cgagagactg gggcgcgatt ccgccctccg
      661 ggacggcgga tccagagtca gctatatcta tctggagggc aactttaatt tcagccggag
      721 gctgaatcgt gatcaagaga tgtccagaac acggattttt ccggggggag agctgcgcct
      781 gcggcagcgc cggtcagatc gtcctcgaag aggagagaag cgagaggctc ggcaggttgg
      841 tggcgggcgc cctcaggcac ttccccgacg atctgggcct ggtgatggac cctcggggat
      901 gggtggacct ggacgccctc tccgaggcga tcgggacgag gtaccgctgg gcgaataaaa
      961 ggctggtgat agcccttgtc caatccgatc ccaaggagag gtacgagatc cgggagggga
     1021 agatcagggc gaagtacggc cactcggtgg gggtaagcct cgactaccca aagaaccggc
     1081 tggcatccct ctactacggg gcgaacgagg aggaggcgga caggatcctg gaagtggggc
     1141 tgaaggcggc aacccagcgt tacgtccacc tctccacgac gccggagaag gcgtggcacg
     1201 tcggaacctt caggacgaac agcccccggg tgatcagggt cgatgccgag gcggcgatga
     1261 gggccggggt caagatgatg accgtgagcc cggatatcgt catctccgag aacgtccccc
     1321 ccgagcacct cagccccgtc cccttctccc atccgagctc cgaggggtga tcatctccca
     1381 aaccgccgct ctctcctctg atagtccctg acggccagga gaaagtcgac cttcttcata
     1441 ccctcccagg atcggtcgat gaagtagagc tccgagtagg ccgactgcca gatcatgaag
     1501 tcactcagct cccgagggcc gaggcgtatc agaaggtcgg ggcggagctt aagccggagc
     1561 ctcgcctcga tggcggcctc gtcgacggcc tccgggtcga ggagccccac ccccacctct
     1621 tcgaggaggg acctgatcgc ccccgtcacc tcctcccttc ctccaggccc cgcagatacg
     1681 acgaggtgga gggggccacc cctcccaaac ctcgcctcgc cttctcgata gaccaacctc
     1741 ccctccgccg ttacctccgc catatccctc gcaagatcct cgatcgcccc gacggccggg
     1801 atctccgaga agatggcggc ctcgcggacc cccagggagg tgcaccaccc cgccacctcc
     1861 ccgaccctcc gccaccagcg gccctccacc gcctcggcgg agatgaccaa ggctatgcag
     1921 gaggggacct ctccccccct gatatccctc tcaaggatcc tttcgcggaa gcggtcgagc
     1981 atcggggcac caggacggtc tctcggagag aaagtatttt tgcgatcggc ttcatctagc
     2041 ggcccatgaa gagagaggcg gtggcgaggg cgtgcgtggg gatgacggtc ctcctcttcc
     2101 cttacgctgg ggtctattcc atcctcccct tatccgcctt attcctgatg gagaggagga
     2161 tccgggtcct cgccgcctcc ctcgccttcc tcctcacctt gagcttcgcc ctggaggcga
     2221 cgagcctcca cctccccctt tacctggtgg cggtatcctt cgtagccccc accttcgggg
     2281 ccctcgtctc ggataggttc aaagatctcg ccaagagcct cgcctacctg gcgacgacga
     2341 cggccctggg gtacgtcgcc atcctctgga cgacccttcc cccggccttg gggacaggtc
     2401 ctgccctcgg agccgggata gagccggaga ggatcctctt cctcgcgacc ctgggggggc
     2461 tctccggcgc cttcctccat accgcctcca cctcccggga cttcgccgtc cccttcggct
     2521 cctgcatggt gatgtggctc ttttgcttcg actatcctct tccggatctc caatacatgg
     2581 ctggcatata cctcttcgcc ctggccctgg ggatcggcgc ctacaggatg aaggccgccg
     2641 acgccgaggc ggtcatcagc gaggtgataa tctgcctcct cgtcatcttc ttcgccgaca
     2701 tcttctggtt cctcctcctc ctctccttct accttttggg gagcggcttc acccgctaca
     2761 agtacgcgaa gaagcaggag cttcggatcg cccaggcccg gggaggggtc cgggggtaca
     2821 agaacgtcta cagcaacagc ctcgtacccc tggcgatggc ggtcctctac ggggtctacg
     2881 ggtcagacct cttcgccttc gccttcctgg ggtcggtcgc caccgccacc ggagacaccc
     2941 tggcaagcga gatcggcgag acggcccgat ccaagccgag gatgatcacg aacctccgct
     3001 ccgtcgagcc gggggtcgat ggaggcgtca ccctcctcgg cgaggccgcc tccctcttcg
     3061 gcgccctctt caccggcggc cttgcgatcg caacggggat ggccgggtac ggcggcctcg
     3121 ccgccgccct cctggggggg tttctcggga cgaacttcga tagcctcctc ggcgccaccc
     3181 tccagtcgcg gggcctcctc agcaacaacg gcgtcaacct cttcgcgacc ctcttcggcg
     3241 ccctggtggg ggcggcggtc tgggccatca tctgagatcc cctggagatc ccgaggccga
     3301 tatacctcag ccccacctca aagctcgatt ttctccctct ccgatccgcc accctcttcg
     3361 ctggggacga cgacccggat cgtgttgagg aggctctcgg tcaccgccac cgacgcctcg
     3421 gaggcgatgg tgcagtcgac ctgagcatag agcgtaccgt tgacgtcccg gatgtcgggc
     3481 cagtagacgg cgcagacgag gatcgcgccg ctgggcctct ccccgaggcc caggatagcg
     3541 ggctttccgt cgatctgcac ctcatgggtc tggatgctgg tgcagttggc gttgttcagg
     3601 tagtacgtca cagccctcct ggtgagggag ctcgtcgcct ccatcgggac gccgtagtcg
     3661 gtgagggcga tcatcatcgg accgtctgca taaatccagc atacgtgagc tggatactcc
     3721 tcgccgtcga cggtggcgta aggtacctcc tcaaacccca gagagtaccc ctcataatcc
     3781 ccgaggtcga aggagacctc gtaccccccc agagtgacgt tctctccggc ggcggaggcg
     3841 gccgccgcca ggaggaggcc ggcgaggaag atcgagagga cggtccgacc cccctttcct
     3901 ctgccgcaga aaccgccctc gatgctccat agatcgacga tccccttcga cccaggctcc
     3961 aaaacccgat acaatccagt catcgaaccc ttccttccat tttcagggag ggggattgat
     4021 cgcatctata ataagtgtta tatcggaggc tctagagacc cctcctccga gccgacggat
     4081 ccggtcatac gtcccgggtc aggccgacgg atcagacctt cgcccctgga ccgaggggat
     4141 cgatcgcggc ggccgaaccc gatgccgaag agccgacgga tccgatcgag ccccgaagat
     4201 ggaggacgaa gagatgaccc tgagaaaaca cgtcagcctg aacatcaacg tccggggagt
     4261 cgagccctcg ccgaccctgg cgatcaacga aaggtgcaag ctcctagaaa gccaggggga
     4321 gagggtcgca aacctcggcc tcggccagtc ccccttcccc gtccccacgc cggtcgtcaa
     4381 cgccctccgc ctctacgcct ccgagaagga ctacctcccg gtgaaggggc ttcctgccct
     4441 gagggaggcg gtggccgact tccaccggcg aaaggacggc gtcagcgcga gccccgagaa
     4501 cgtcctcgtc ggccccggct ccaaggagct gatgttcctc ctccagctcg tctactacgg
     4561 cgagatcatc gtccccaccc ccgcctgggt ctcctacgtc ccccaggccc agatcctggg
     4621 aaagaaggtc cgcctcctcc acaccacctt cgacgacaag tggcacataa cctccgagag
     4681 gttggcccgg ttcctggagg gggagcagga cgtctaccgg ccgaggatcc tcgtcctgaa
     4741 ctacccggga aacccagacg gcctcaccta ttcgccggag gagctggagg agatcgcgag
     4801 ggtcgcccgg gagtacgagc tggtcctact atccgacgag atctacggcc agctccacca
     4861 ccggggcgag cacgtctctg tcgcgcggtt ctaccccgag gggacgatca taagctcggg
     4921 cctctccaag tggtgtggcg ccggggggtg gaggctcggg accttcacct tccccccgga
     4981 tatggactgg ctcctggagg cgatggccgc cgtcgccagc gagacctaca cctccgtctc
     5041 cgccccgatc cagttcgccg ccgtccaggc ctttcggggc gggatagcca tcgagaggta
     5101 cctctggcac gcccggagga tcttatcgac cctcggctcg gagttctacg ccgtcctcag
     5161 ggaggcgggc gcccgggtcc acgccccgga gggggggttc tacctcttcg tcgacctctc
     5221 gcccctggcg gatcgcctct cgagacgggg gatccgggac ggcgccaccc tctgcgagca
     5281 gctcctcgcg gatagaaagg tggcgatcct cccagggtcg gccttcggcc gccctaatag
     5341 ggagctatcg gcccggatcg cctacgtcaa cttcgacggc tccaaggccc tcgccgccag
     5401 cgagacgatc cccctccacc agaagctccc cgaggatttc atagaccaga tctgcgacga
     5461 gacgatcagc gccgccggcg agatggcgag ctggctgaga gaatagatcg cggggctata
     5521 aaccgcaagg gcattccacc ctcggaaggg taagaatacg aaataaaatt ggaggggatg
     5581 ggcgaatcgg cccatccccc tagagcgcct cgtctctttt tcgtctattc actcgcccgc
     5641 ctgcatgatc ctgccgatga tcctgccgta ggcgggcctg gctatcaaga cgccgatgag
     5701 gacccccgct atggtgacca gggcgaagct cttgaaggct ccaaagccca tggcgaatag
     5761 cgggagcatc gctacgatcg tcgtcgcagc ggcggccatg atgatcccga aggcgctgga
     5821 gagcctggcg aggtagatct tgccgctggg catcttcccc tcgtagagga gctcgtcggt
     5881 gatgatgacg aggtggtcta cgccggtccc tatgacggcg atgatcccgg cgatggcagc
     5941 cagatcaagc tgccacttca gcagtgccgc cagggcgagc atcatcagga cctcgctgaa
     6001 ggaggtggcg accatcggga ttacgatcct gggatcgcgg taccggtagt atatcacaag
     6061 gcccaccgcc aggatcgcta tgatcccggc tatcaggacc tgctttttga actggtcccc
     6121 cagggcggcc gtcacctggc cggatccgat gatctccacg ttgacgggga gggccccctc
     6181 cctcaggtgg atgtagagct cgtgagccct ctggctgtcc cctcccacct gggccaccat
     6241 attcctcatg ggggccacct ccaggctcgc cgccaggtcc tgggcgaggg gggcggagaa
     6301 gatcaggtcg ttgtccaggt tcatcgagat ctcgtgctcc atccggttcc tcgtagcccc
     6361 cgtatccagg gcagccttct ggaaggccac cgccccgtca tccgagaggg tgaaggggac
     6421 gccccagttt ccagactggt ctcccgtagg gagggatacg tcggcgatct ggtcgccgta
     6481 gaggacgtgc atcgactcgt tgccagtggt ctggatcctg atctcgaaga ccccgggctt
     6541 nntgttgtcc accaccctga ccttgatgtc cgcaaggccg aggcggttca gcttcctgtc
     6601 caggacttgc ttcgtctcgt cgaccgtctc ctcggtcacc ccctcagaga agcccgcctc
     6661 cccgggggcg agggtcccgc ctacatcggc taggatggcc tccagggcct ccctggtgac
     6721 gttcgacctc acctcgaagg atatggggtc cgactccagg tacttgacgt ccgagtcgag
     6781 ggaggtcttg aggtagttca tgatcacgcc gatcggcgag gcctcgaggg tcacggcggt
     6841 ggcgttgccc cgcggagtga cggtcgcctg gggatatccc agattctcgg cgacggtcct
     6901 cgccgccctc ccctcgaagg tgatctgata gtcgtagcta ctggggcgcc actccacctc
     6961 gacgggctgg ccgagggagt cgctgaactg cttcttcagg atgttgaccg gatcgacctg
     7021 gggctggact atcgctccct gaagcttgag ctgcagccat gatccgccct ccaggtcgag
     7081 gccgtacttc agcctcgaat ggaagccgtc ttccgagggc atggggatca ggatcaccag
     7141 ggccgcggcc accgccacga agaagatcat gatcctgggg tcttttgtga tctctgtcat
     7201 cttctcttcc tccccgaggt cgacttcttc ggacgggcca gataccatcg aagggcgttg
     7261 gagttcatca cccaggtgtt catcagaccg gcgagaaggc cgaagaggag gaccgtcgat
     7321 atgtcaaaga ggatgttgat ctgggtgaag gaggacgata ctaggtagga gaaggtggag
     7381 acgaggaata gggcgaagat ggccgagaag gtggtgctgg tcatggcgat cccggtcctg
     7441 aaggctccca ccaccttatc ctcggtcctc cccttccgct ttagaaccct gttgttgagg
     7501 aggatgttgc tgtccacgga gtagcctatc agcatcagca gggctgccac cgtccccagg
     7561 gagagcttca ccccggcgat gttcatgcag gcagcggtga tcatgatgtc ggagaaggcg
     7621 gcgaggatca ccgtcagggc gacgaagggc gtcctgaaga tcaggaagat taccaccgcc
     7681 atgaagagga aggatagcgg aatgtacttc ttcgcctgct cctggaggcg ggtgctgtag
     7741 accggcccca tgtacttgat ctcgggttcc tcgtactcag cttctatcat cgccgaaagc
     7801 tcccgatatt tggcttcgtc catgggcccc agctggatca tgtaccgatg gccgacgtcc
     7861 ctgatgtccg tcaccgggta attcgccagc ttcgcggcta tctcgccctc cgattcagct
     7921 acggggacgg tgattatcgt ccctcccgtg aactcgaccc ccaggttgac gggggatccc
     7981 gtcttcaagt aggtgaagcc caggatggag agcgccaccg ccagcacggc gagggagatc
     8041 gccaccatct tcttgggctc gtatttagag atgaaggtct ctggatccat actagtatcc
     8101 tctttcgcac aaaccgcatt caacgtattt acgggttgtc ttttgtccac atttcttcat
     8161 aaattcggat ctacggcggg aggttatttc ttctcccacc tcttgaggtt tgccacctcg
     8221 gagagggtcc ttccccggag gatctcctcc cggagcctgc tctcagtctt ctcgacctct
     8281 ttcgccctcc gggcgatctc gtaccccctc tccctcggga tgacgacgac cccggagtcg
     8341 tcgccgacga tcaagtcgcc ggggcggacc gagaggcctc cgcaggtgat ctcagcgttg
     8401 atctccccca tccccttcgg ctcgccggcc atgggggtga tggagctagc gaagatcggg
     8461 tatcccatct cccggatctc gtcgaggtcc cggacggcgc cgtcgatgac gacccccgcc
     8521 acccctttga tcttgcagct ctgggtggcg aggccgcccc agcaggagac gtacctactc
     8581 ccgttataga tgacgatgac gtcctcgggg ccggcgagat cgatcgcctc caccgacttc
     8641 gcccagtccc cctcgaaggt ctggacggtg accgccctcc cgacgatctt ctggcccggg
     8701 tttatgggca ggacgtcccg catagccccc ttgcggtgca tggcgtcgga gatgttcggg
     8761 gtcgatacct ccctgaagat ctccaacatc tcctcgtcca tgctcttcct ctccctccta
     8821 ataacccctt catgggcccg gtccatcgcc tcccggatct tccggccgga ggcggtgacc
     8881 tcgggactcc gggccacgct ccccccgacg atgacgatgg aggcccccgc agcgatcgcc
     8941 tcggcggcgg agggcgcatc gatcccgccg gcgacggcca ccgggatctt tacgtggccg
     9001 aggatctcgt cgaggaagtc gatcgccctc cggcccatca tctgctggtc gatccccacg
     9061 tggacgcaga tgtaatcgac ccccagggcc tccatctccc gagacctcgt caccgggtcg
     9121 ggggagccga ggaggtcgac catgacctcg accccgtact tcctcgccgc ccggagggcg
     9181 tcgtggacgg tcgagtcgtc ggaggaggcg aggatcgcca cgatgttggc ccccgccttc
     9241 gccgccatct ccacctccat ggcgccggtg tccaccgtct tcatatccgc cacgatcttc
     9301 ttagccggca gggccttctt cagctcgcgc acggcgtcca tcccctcgct cttgatgagg
     9361 ggggttccgg cctcgatcca gtcagcaccg ccgtcggccg cctccagagc tatctggatt
     9421 gccctgtcca gctccagcag gtccagggca acctgtagaa tgggtttaat aagatcacca
     9481 ccataaagcc gcgttgctag agaacagacc atggtatata aaatgaagct gaaggagatg
     9541 actgatgcgg gggtgaaggg atctccggcc agctcaagcc ggatcgcctt caccaagctc
     9601 caggggaacg gcaacgactt cgtactgata gacgagcggg atggggtgaa ggtggccgac
     9661 cccctcaagg gctcctttgc cgtccagtgc tgccaccgga acttcgggat cggaggcgac
     9721 ggagttctct tcctgggggc gtcggagcat gcggacctga ggatgcgcct cttccagccc
     9781 gacggctccg aggcggagat gtgcggaaac ggcgttcggt gtctcgccaa gtacgcctgg
     9841 gactccggga tggtggggga gtccttcgag gtggagaccc tggcggggat catacccctg
     9901 atggtgaggg ggatcggggg cgacttctgg gcccgggtag atatgggcgt cccctgcttc
     9961 gaccggaggg ggatccccgc cctcggcgac ggggagttcc tgatggagcc cctggagggg
    10021 ttcgaggtat cggcggtgaa caccggcgtt ccccacgccg tcatcttcgt cgaagacctt
    10081 ttgaacctcc ccctcgagga ggtcgccccc cacatccggc ggagcagcct ctttccggag
    10141 ggagcgaacg tgaacttcgt caggataggc gactccttcc aggtgaggac cttcgagcgg
    10201 ggggtggagg gggagaccct ctcctgcggg acggggtcgg tggccagcgc cgccgtagcc
    10261 cggaggatgg ggatggtcgg cgacgaggtc ttcgtcgaga cgaggggcgg ccccctcctg
    10321 ataaccttcg acggcgatag ggcctacatg gacgggccgg cggccaccgt cttcgccgga
    10381 gagctgagcg aggagttcca ctgggatatc tcgcaagagt tataggggat tagcttcaca
    10441 gggtctccat gatgaagcct gtagtggcgt tggtggcagt tctgctcttg gcggggacgg
    10501 ccctcgcctt ccaggggccc gagccgatga ggcttgggag caagagcgac ccggaggcgt
    10561 ccctcttctc ctacgaccag aagttcagcg ataccaccac cctcaagaac tactacctgg
    10621 ggatgtccgc ctacaaggac ttcgacgggt gcgagaggct ctcgtcggat atcgccctcc
    10681 agagcaaccc cgactgggtc gatatcgcca tccaggcgaa cttcagccgg ggcaccggaa
    10741 ggatggggta cctcgtcctc aaccaggaga cgatggacat cagggacgtg atggtggacg
    10801 tcagccacga gttcatcggc aacttcagca tagacgagaa gatcctggtg gtgaagagcg
    10861 ggatgaacga gacgaacctc ttcggctggc tccgataaac caccccggcc aggagccgcc
    10921 ctcctccccg tcacggtcgg agctgctctc cgacgatggc gtccaggatc tccaaaaact
    10981 ccgccctcgc ctccgggggg atcgtcgccc cagcggcgac gttatgccct ccgcccaccc
    11041 cccccacctc ttctgcggcc cggaagatcg cctccgccag gttcagcccc gacctcacca
    11101 ggtcctgggt cccccgggcc gagaccttca gcctcccgtc ctcggcggcg gcgaaggcga
    11161 tgatcggctt cctcctgtca gcggtctgaa agctcatccc ggcgatgatc ccgacgatgg
    11221 tatccaggat cctgtctttg gcatcgaagt actggatgtg ggataagggg acgatcccct
    11281 cctccttcac cagcttcagg ccgtttacca ggttctgccg atgctcggcg agaagaagcc
    11341 tcgcctcctc cagcccccgg ccccggtccc cgaggcagac cgcaaggccg acccccgagt
    11401 ggccgtaccg ggcggtggcg ttgaggaggg tcgagtactc gctcgcgtcc cggagctccg
    11461 tcccctcctg ctcggagagg agggtgtaga cctccccgac gagcctctcc agctgggcgt
    11521 tggagaggcc ggcccggagg ccccgtcgca ccagggccga ggcgatcctc cccttctcct
    11581 cggggctcat gtcgatccaa cgccgccacc tctctccctt cagcctgatc ccctgctcct
    11641 tcagaaagga tatgcaggcg tcctcgtccc ccgagaggcc cggtatatac ggatcggagg
    11701 agaactccag catcttgaag accggcctgg tctgctttcc aaagagcttc aggtcgcggc
    11761 aaaaggagag gacctcagcc ccgaccccca cctccaggac ccaccggttg aggcctacga
    11821 gcctccccct cgccatgtcc tggaggtccc cgacggcgcc cactatcgcc aaggccgata
    11881 ggtcatcgtt tccgcctccg gagccgacgg ccctggcgag ggcccgggcg aggaggaagg
    11941 aggagccgct cccggagagg tgggtggcgc cgtcgaggcc gactaggtgg gggtttatgt
    12001 ggagctccac ccccgacccc tgggggcggt ggtggtcggc gacgaccgcc gataggccca
    12061 tctcgaggat ctccgcctgc atcccggagc cgaggtcggt gaagatgacg aggtcggggc
    12121 cgaggtcggc gatctcctcc agggcgggga ggtccagctg cctgaagaag agggtcttgc
    12181 ggtctacccc cgcccgatcg agggcggtcg agattatacc ggcggaggtg aggccgtcgg
    12241 cgtcgacgtg ggaggcgacg accacctcct tctggcggac tatagattca gcggccctct
    12301 ccgagatccg ggcgagggtc gagagctcgt cgtcaaaccc cgccccttct gcatcggtca
    12361 tcccttctcc atcgcccccg gcctctttag acctttcgct gaggtgacta ctccccgccc
    12421 tgaagggcgg agcttctagc ttttggcaga gactgtagtc ccagtctcaa aatgtttatc
    12481 gccgcattct cgtcacggtc catgaccagc ccgcattgag gacattcatg gacccgatcc
    12541 gatagcgact tcgtgacttt aagaccgcac ctggaacata gctgacttgt gttcccggga
    12601 tctactagaa ccactttcga ac
//
LOCUS       DASM01000114           10310 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_181442, whole genome shotgun sequence.
ACCESSION   DASM01000114 DASM01000000
VERSION     DASM01000114.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10310)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_181442"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    9467..9490
                     /estimated_length=24
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2035 a         3004 c         3022 g         2223 t
ORIGIN      
        1 accgctgccg ctctgacggc gggctccccg tccttcagag cctcgatgag aggatcgata
       61 gcctccgatg cacccgatcc gccgaggacc gttgccgcga tcagccggac agtggcgttc
      121 tcatctccta gagcggctat cagggggccg accgccctcg gatcttcgat ccttcccagg
      181 gcctctgccg ccatcgccct gacggaccag ttcccatctc cgagggcctc ggcaaggtga
      241 tcgacggccc cggcccctct tatctctccc aggagggcgg cggcgatgac ccggacggtg
      301 gcgtcctcat ccccgagaga ggctattaga gggcccatcg ccttctcaga atcgatctcg
      361 acaagggcgc ggacggccgc ggccctgacg gcaggatcgc catctccgag ggcgtcagtg
      421 agaggctcga aacccctcgg atcagcgagc cttcccaggg cggctgccgc cagggtccgg
      481 acggccgggt cctcgtccag aagagcgccg atgacgggat cgaccgctct atggtctccg
      541 atgccggcca gggcgatcac cgcagccccc cggacctccc attcgccgtc cttgagggct
      601 tcgatgaggg gtccgacggc cctcgcatcg cccatctctc ccagagagag ggcggcagcc
      661 accttctccc cgggatcgcc gtgctccaga tcgtataaga ggcgggatag ctcgtcatct
      721 tcttctgacg ccccatcttt ttcctcttct ttttcttctt cggccccttc atcatcgccg
      781 gtagcttcaa ttatctttcc cagggcgtcg gatgccttcg atctgaccca cgggtcctca
      841 tcccggaggg cttctctgag ggggacgata gccctcggat ctccgatatc cccaagatgg
      901 gtcgcagccc acccccggac ccatttgtcc tcgtcctcga gggccaggat cacccggtcc
      961 agatattcgg gcctgccgag cttcacaaga gagcttgctg catggagtcg gaccatggag
     1021 acctcatcgg atagggcctc gatgaggggg tcgatcgccc gggggtcgcc cagatttccg
     1081 aggcttgctg ccccgccggc ccgcagcccc cattccgggt ccttcaggta agctatggca
     1141 tcgtccacct ggtttgcggc gaccaggccc gccagagccg cgatgatcaa taatacaaaa
     1201 atcggcttaa gtttcatatc ggcatcccct gtcaatagga gacggtcctg aagattgaaa
     1261 agtatatcgt cgcctcagaa ctataaacag cctgaaacga acagctggaa acggcccgcc
     1321 ggatgccccc atctaataga gatctcttca tctccggcga ccccggtccg tccgaccgcc
     1381 atatcctaac ccactgatat ccgcaacccc tcgatatccg caagctcccg atatccctat
     1441 cccctttata tccgcaaccg ctaaatcctc cggaggatat tttgaagatg gtgccatgac
     1501 cctcgaagat aagcccctcg acctcctaga ggagttggac atcagggccc ttttagacga
     1561 cgaggtcccg gagagcaagg tcatcgacta caagaggcgg cttcccggcc cctcggacct
     1621 ggagaagagg gagtttctcc gggacgtcac ctcgtttgcc aacgcctccg gggggcacct
     1681 cgtctacggg gtcgccgagg agaacggcgt ccccgtgagg atcccggggc tccccgggat
     1741 cgaccccgac caggagatcc tccgcctcga cagcatggtc cagacgggga tcgccccccg
     1801 gatccccggg atatccatga gggccgtccc cctgaagggg aaacgtttcg tcatcgtcgt
     1861 ccggatcccc aaaagctggg cctcccccca catgatcacc aagggatcct ccaagttcta
     1921 ctctcgaaac tcagctggaa agtatcccct cgacgtcttc gagatcagga ccgccttctc
     1981 cctctcgggg tccgccgccg agaggatgag gagcttccgg gccgaggccc tggggagggt
     2041 cgtcgcgggg gagactccgg cggccgtcga gccggggccg aagatggtct tctccgtcat
     2101 ccctctgggg gccttcgagc ctgccgccag gttcgacgtc tcctccatag tacagaggta
     2161 caggagcggg aagggggccc tccccctccc cctgacgggg ctcttctccg gctggcggta
     2221 caacttcgac ggcctcctca cctacgacga ccgaggctcc tacgtccagg tctacgagag
     2281 cgggatcgtc gaggccgtcg actccgtcat cctcggaggc atcggtaggg agatcccggg
     2341 cgtccggtac gaggaggcgc tcctggaggc cctcccccgg tacgcccggt tcctggaatt
     2401 cctaggcgtc gagcctccca tcttcaccac cctcaccctt ttgggggtga aggggtgcac
     2461 cctcagggcc gagggctccg acgcccccgg ggaggtcgag atcgaccggg acgccctcct
     2521 cgtccccgag atcatgatcg agggcttcga ctacgacgcc gaggatgtcc tgaggcccgc
     2581 cttcgacgcc gtctggcggg ccgccggctg ggagcggtcg atgaactacg actcgaaagg
     2641 ccaccggatc gggaggagat gaatcaggtc gtcgaggggc tggagtggat ggataggtaa
     2701 gccctcttct ccggatgcga tagggttggg ggtcctcctg accgacccat ccggttcttg
     2761 cgctccggag aagagcctcg gtctctacag cttctgcggc gagctcgaag gcttgcggaa
     2821 acctcggctg gcgggggggg atcggggcga ggagaagaag gatgccatga ggagggcgaa
     2881 gagggtctcc agggaggagt acgtcagggc cctccgggga gggaagtcgg gggagggcgg
     2941 aactcccttt ggcgatcgtc tcccagggcg gtcgtctcac aggtagatgg ggacgtccca
     3001 cttatcctgc ctctcctggg gggcgttctt cttagaccag gcggggtaga ttatgacgtt
     3061 gccgaattcg tcctcctctc ggatcatctc ttccttttct tctttctctc ccctctctcc
     3121 caattcttcg tcgctcatgg aagtttcccc accaggctgt tgccgtctga ccagaaacgg
     3181 ccttcttttc tcggaggggt tttaacccgg cccaaagaat ttaaggtttg ccccgttcga
     3241 tctcagcctt cgatctcagg ttctgggatt ttgtccctga ggatatcggt tcatcctggg
     3301 gtcgataaca tatatatctc atttctctca aaagatgcca gcactatagt ggagggtgaa
     3361 gtgagatgac ggagacgacg gtcgaatcga tatttggctc caaggcgggg ctcgtctggg
     3421 acgccctgaa cgagaacggg ccgagcacca tcaccgagat catgaaggcc acctccctca
     3481 ggcgggagga tgtctacggc gccctgggat ggctggggcg ggagaacaag atcgccgtgg
     3541 agatgcgggg gagggcgagg gtcttctcct tgcaggcgtg gtgactgaag accggatagt
     3601 tacatctgta taaagggctg ttggaccgtt ccgctgctgg gggtcgggga gcaggcggcc
     3661 tcggccgcca ggggcgttct tgcagggatg gcggagcagc ggaaccgcgc ccattggtac
     3721 gttttttagg tctgcgacat ttattgctac cgacctggcc gccgttgtga tgatggctta
     3781 ttcgagcccg gttggagcag ccccctcctc ggagacgggg ccgtttatgt ccagagggtg
     3841 atcaattgca gcatttagga gaagtcggcc gggggcgtgc gcgatgggct gccccgggaa
     3901 aaagaggctc gggtccgggg atctttgtcc caatcgcccc gatggcggct ctggagcgag
     3961 atcccaagcc ccggagccgc cagaaccgtt tctattaata tctcttaaaa attggactct
     4021 tttaatactc attattcgat ctaacgaggt taagtaaaat taatatctcc gaagatcctc
     4081 ataatggcta caaatatcgc tcttttctcc ccacaaaatg ggatcttttt ccggaaattg
     4141 gtcacaaaaa ggactttata gccggagcca agagaaggat atagtgcagt atagaaccaa
     4201 ggaggtgaga atcgaatgtg ttgccatact ggagataagc atgcccattc ccacgatcac
     4261 gatgggaagg tccacaccca tgagcacgtc cacgacgccg accatcacaa gcacacccac
     4321 aagtgctgtc acggccacta gcccctcgat cggcggctca atttctggcg aacgaagccc
     4381 tgtactctcc tttcgggcgt cgggtctccg gacccgggcc gccagggctg ccattcttta
     4441 attacagcaa aattttgtga tcctctcctt cgagttcctc tcgatttttc ctatcagctc
     4501 tccgggatgg ggtcatccaa taatcctaaa acgccttatt tctacaaaga ttggctatta
     4561 tgagggaaat tgttaataag gatccgagag aagtttgatt gtgggcataa taccataggt
     4621 cgtgaggagg catcgacgga ccttcatacg cctccagagg ggtgacatct ttcgagttac
     4681 gagacggatt tgatctcgaa tttcgtgatc ttcaatctcg tatttttatc taggaagtta
     4741 tgaataatgg atgtggtggt cgggatgaag gaagagagag cgggcaggct tgaaggacgg
     4801 acggcgatag tgacaggctc gacttacggc ataggggagg cgatagcgaa gatcctcgcc
     4861 ggggaggggg cgatctccat cgtcaccggc aggtcggagg atgaggggga gcgggtggtc
     4921 gaggagatca gatctggagg cggcgctgca gagtactaca gcctcgacgt cactagtgag
     4981 aagagggtcg aggaggtggc cagggccgtc tacgagggct acgggaggat cgacatcctc
     5041 gtcaacaacg cggggatcgg cggccccagc aaaccgaccc acgagtacac caggagagaa
     5101 tgggagcggg tcttcgacgt caacgtcacg ggagccttcc tctgcacaaa gcacgtcatc
     5161 ccctacatga agaggggtgg cggcggaaat atcgtctaca tctcctctat atacgggatc
     5221 gtcgggtccc aggacaaccc cgcctaccac gcgacgaagg ccgcaaacag gatcatggcc
     5281 aagacgggcg ccctcattta tgcgaaggac aatataaggg tgaactcggt ccatcctggc
     5341 ttcatctgga cgccgatggt cgaggagttc ctaatagagc agtcgaagga gctgggggtg
     5401 cccctcgagg atctgaagag ggagcttggg gcaaggcatc cgataggcca cctcggagag
     5461 ccggaagata tcggctacgg cgtcctttac ctcgtatccg acgaggccaa gttcgtcacc
     5521 ggctcggagt tgatcataga tgggggctac accagcaggt gatcctcagg tccggcgggc
     5581 ggacgacctt cgagaagagg ttcggggtga ggaagctatc catggtatgg aggatgagct
     5641 tggaggctaa agggggcttg cgaggttcgt ggaggcgaaa ggggctgaaa cgcccttttc
     5701 cgtcgattta aacccccccc ggcggcggga cggtcttttc catatgccac cattctttga
     5761 agaggagatg aagagggttg aggagtcgat agaggactat cgagagggca tacggaggaa
     5821 cgttgccatc atctcggacc ccttctatgg cgaggcggat ctcatggccc gggccgcctc
     5881 gatggcgggc tctgacggcc tcaggatcga cggtcggagg ctcaggcacg acttcgggat
     5941 tctggagagg gcgccccaga ggatactgct ggtggatgag gcccaccggc tcagcctgcg
     6001 gaggattggg ggctttgatc tgatgaaccg ctttttgagc ttcgtcacct cggaagagag
     6061 gctcttcatc accacttgga acagcttctc ctggaggtac cttgaggagg tcctgggtct
     6121 cggccggcat tttccccgcc agatcatctt gcccaggctg gatgcaggcc agatccgaga
     6181 gatgctcctc gccggccgca gagagggcga gcttaccttc gtcgaggaag aggcaccggg
     6241 aggggattcg aaggtcttgg actggcgcgt ctccagaagg gtcgtgatgg gccataatat
     6301 cgatctcccc tacccctcgg tggacccgga aatgatccgg tcgcgcctaa taaggaacag
     6361 aaaagagaag gagtccgccg aagatgcctt cttcgagagg cttgcccgga tctccgacgg
     6421 caaccccggc gtcgcggagc atctctggaa ggaggccctg gactatccgg aggtgagaag
     6481 ctccctgagg gatcctccca ccatcgacct tgacttcgac gagtccttcg tcctgggagt
     6541 cgtcctctct tcggggtctc tggagctgga tgagctctcc gaggtgctgg gccccgtgga
     6601 cctctccgcc gagatgatgg cggccattct ggaggggaag gggctcgtct ccatcgatgg
     6661 gggtaccgtc accttgaagc ccgaggctct gaagagcgac attgagcatg agagaaggct
     6721 caggctggtc tggtgatgat gggcgaggag atggcggcgg tggccccgga gtcgtcctcg
     6781 aacctctcgg tgtcttctcc ctcggagggg gtcggcgagg cctccgaagc gatctcctcg
     6841 gccctccagg ccttcgggat cgactcctcc accatcgtcc acgtcatcct cgtcctggtc
     6901 ctcgcctacg tggcgacccg gatcgtcgcc ttcctcctga cgtggttcag tgagaggttc
     6961 cactggctcc gcctcgggat caagatggag atccctctcg cgaagctcgc tatctacggc
     7021 ttcgccgtat accacatcct cggatctgtc ttccagctct caacaaatca gctcatagcc
     7081 ttctcggggc tcatcggcgc cgccgtgggc ttcggcctga aggatctcgt cgccgaggtt
     7141 gttggcggga tcgtcatcgt cctggacaag ccctaccaga tcggcgacaa ggtgagggtc
     7201 gatggccact acggcgaggt gacggacatc ggcctccggg cgacgaggct cgtctccccc
     7261 gacgacagcc tgatcgtcgt ctcaaatcag cggatgatca gccaggccct cgccaacgcc
     7321 aacgccggga gcctggagat gcaggtcgtc atcgacctct tcatcgactc gagctctgat
     7381 gcctccaggg cgatgaagat cctgagggag gcggccgtca cctcccggta cgtctacatc
     7441 tccagcaagc ggccggtgac gatcctcctc aaggaccgcc ccttctacag gagggtgagg
     7501 gccagggtct acgtcaacga tctcaggtac gagttcaagt tcgagtcgga cgtgacgaag
     7561 cgggcctggg acgccttcct ggaggcgggg attgagcccc cgaaggcgat ggtgatggac
     7621 ccggaggggg ccgcctcccc ggcggcgtag gctcccccgg acgccgcccg gagatgcggg
     7681 ccggaatcac cgccaagggc cttcgagggt tcttgtgggc tgtccattct attggtcgag
     7741 ttggcgtttc catctcggcg agggttgggt cccagcattc tgcagagcat gcggccgggc
     7801 gggcggcgaa agccaaggag tcggcctcca gcccaactat tttcagcaaa ttttataaat
     7861 cagcatcaat acacccggcg gcgccgaagg ccccgcaggc cagaagccgc cgcccgtcgg
     7921 ccggaaccgc gaggtatccg cccgccgagc cggtgctggc aaaagggctg gccgggatcg
     7981 gccgcccggg gccggagagg gcgaggaggc gagggaggag cggaccgccg accccgcgcc
     8041 cctgccagta ttccgaaaga aagggcgtgc aggatcgggg cggcgtcgca ggcggtgtgg
     8101 atttccatca cgccgatgag gtccccagaa tcgaaatgta ttcacaattc tgtgccaaga
     8161 atctcttttc ttttctgggc gttagacagt tctgatatta gaaatttcac ccattcttct
     8221 gtatacacat aatcgttatg tgcagcatca taatggcaat atcttgtatc ggttttctct
     8281 ggactttcac ttccacgttc cggtcgaact ccataatatt tccagcattg cgcatgtcta
     8341 caactcgcat caaattttgg tatgctaagt gtcgctttta tcctctctgc tacgtctccc
     8401 ggctttaatt ttcctgggtt tacaacaggt acttgttttt cttttataat cgctgttatt
     8461 cttttatatt ccttcatttc ttccggctta ttttggtcgt aagggacaaa ctctattgcc
     8521 aaatctgatg atgatttccg atttcctatt tttgggatta agaacacttt aaagctgtat
     8581 tctgggtcgt cccatacaac atcatctaaa gaatttctaa atgtgtccac gtactctttg
     8641 atcgttagat attctctaga ttgaatctcc tttaaaactt gatttcgcca atttgggtta
     8701 atagatacca actgaagggg tattgctaac gattcgttta gactaaaacc ctgaccaaac
     8761 tcttgcgtga ttagcttttc ataattaagc aataatgctt ggcattcacc gcagatttcc
     8821 aaatctaatg ctggtaagaa cttgtgttct atttggttcc taagacctac aaagaatttt
     8881 aaattctctc gtgatggtgg attttgttct ttaaagtatt cttttacgca tttacttata
     8941 tcccatgcct ttctttcgcc atccacataa tgatatctat ttgaattcgg cttcttataa
     9001 tgatatttta taccatctcg ttcaaatatt gcaaggaata acgaagtcca agcaatattc
     9061 atcatgacaa taaagccata agacctaaac ttagtggatg gtttattata aatatctaca
     9121 gctaatattg ctgagtctcg cgattttgtt aataattctc ttactttctt tggcaatctt
     9181 ggcatatgtg taacaggtat aaaaaacact tagatctatt tctggttttc gcctcacctc
     9241 caacaagtgc cgaacctcag caaaattcac atccccatca cccgctccgc cgcctccaaa
     9301 aggtcgtgga cgacgtgggg ccccgactcc atccggtccc ggtcgaggta gagggcggag
     9361 agccccgcct cctttgggac ctcgtagtcg aagtagcggt ggtcgccgat gtggatcacc
     9421 tcttcgggct tcgccccgag gtggcggcag aggtcgagga aggtctnnnn nnnnnnnnnn
     9481 nnnnnnnnnn cgaactccac gaactcggtg gcgccattgg aggcgacgac caggtcgaag
     9541 tcttcgctga ggagggcgag gacctcctcg gtctcggggt agagctcgac atcctgccgg
     9601 tacatgttga ggaggtcggc gtgggggatg tcgaggtcga agcggcccag ccagtgggag
     9661 aggtcgtacc agcgcaggtc ccgggttccg acggacatgt actcgtcgta tacgagcttt
     9721 tgggcctcct ccatcccgat cccgtgccgc tgggcgtaga gggtggggac cccctccagc
     9781 cagaccctct cgacgtaccg cgaggtgacg aggnntcgaa ggatacgatc ttgatcatct
     9841 tgaagcacca gttcaggggc atattggccc ccccgccttg ttaagctttt gccgccccgg
     9901 cctccttcat cgccctggcc gccttggtcg cctccgttcc cgtcggcagc gaagctattt
     9961 aaccgaggcg atgcctccgg aggaccaagg agcttggaga gatagatctt tgccgatcga
    10021 taacctcgtc cccgccctga tcctcacctt cctcgcgggc ctcgccaccg gagtcggcgg
    10081 cctcatcgcc tacttcgtcc cccgccccga catgaggtac ctctccgtca gcctcggctt
    10141 cgccaccggg gtcatgatct tcgtcgcctt cgtcgacctc ttctgcagcg ccaaggaggc
    10201 gatagggatg gtccgggccg acctcttctt cctggcgggg atgctcgtcg tctacttttt
    10261 ggacaaggtc gtcccccatt cccatatgga cggccaggcc gacccccact
//
LOCUS       DASM01000115            3412 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_245804, whole genome shotgun sequence.
ACCESSION   DASM01000115 DASM01000000
VERSION     DASM01000115.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_245804"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          692 a         1054 c         1054 g          612 t
ORIGIN      
        1 aacagcttca acggtacctg gggaaccgat gccagcatgc acaagatctt ctacaaggac
       61 ataaagagcc acgaactctt cagcggtgac ttcgaggtcc agaaggagat caagttccac
      121 gagaaccccg tatatgaacc gagaccgtgc ccgtgcgcgg gagtggactg ctgaggtcag
      181 cagcccgccc tcttttctta tttttgcggc ggagccggtc cggatccgac cttcccgaag
      241 gaggaaggcc ttttccgtcg tggcttgaaa ccaaccgcct ttgtcgcaaa gcaactcgtc
      301 atgggggtta aagagtgaag aaatgcctga tgatccttct atttctggcg gcccagacgg
      361 cctttgccac cgtcgaagag acgaggcagg ggccggaatc ggagtggaat ttggaggcgg
      421 ctgccacagg gcccgtatcg ggtccggtat cggggcccgt atggccctct gcaccccgga
      481 tcctgggaga tcttcgcgta tccgtcgatc tctccgggcc gaagtccggc catgaccaga
      541 actacgccac gccgggaaag accctggagt acatcgtcac cgtggagaac ctgggatgca
      601 ccgacgcgga tgtaacgctc gtggccgtcc ccaagaactg cagacccggc tggttcgggt
      661 ggaccgagct ggtcatgacg gtggcggcgg gtagcgtggg gaccgaagtc cttcctgtca
      721 ctcccgatat gaaggcgccg ccgggagact acgacttcca ggtgaccggc tcagccccga
      781 gcttcaagtc ggggtcagcc atcgcggggt tcaagcttca gaactacgac tacgtctccg
      841 agacctccgt cagcggtagc ggtcagttca cgatgaacaa agacgtccgg agcatgaaca
      901 ccggggtcaa gtccaacaag gacatctact tcagcggcag cgtcgacact ctgatgaaga
      961 acgagtacct ggtggagaag gcgaagggag ggaaccccac cttccaggag tgtgatgccg
     1021 tggagaacta cgtagccgat aaccctggag acaccctgat ggggaccgag tcggtcagaa
     1081 gctccatggt cttcgggggg gtgggggcca gggtccacga gagctacaac ttccaggaga
     1141 tggagtacga ggtccagcag atagacctcc accacaccgg cccccaccgg agggcggagt
     1201 tccagacggc ggacaacttc accgggtatc tggccatcga cgccaagcag acgatccccg
     1261 gtcagaggag caccagagaa cgggaagagt tcctggggtc cttcgagatc cagagaaggc
     1321 tgatcttcaa ggacaacccg gccccgagct tcggctgctt tgacggctca tgcttcaact
     1381 tcgccgagag gttcaaaacc atctcgagcg gaggatgagc cgctctttta tttattttat
     1441 aaaattagcg ccgagggagg gattcgaacc ctcgagtccc gaagggacac aggtttagca
     1501 aacctgcgcc ataccaggct tggctacctc gacccgaccc caacttgatc ctctcgatat
     1561 ttaaattctg cgtcgggagg cgtacccgga accaccgatg atcaccgcct gcggtgatct
     1621 gaggtcttct cggagcgaga cgtctcccca gcctccccgt cgctgacgca ccggaggaga
     1681 tctttgcttg tgatgatccc cacgagggcg ccgcattcaa ccaccggcaa cctccttacc
     1741 ttatgctcca gcataatctc cgccgccctt ctggtcgtct cgttggggcc gatggtcacc
     1801 agcggcgccg gggtcatcac ctcccagacg acgacgttcc tgggatctat ccccttggcg
     1861 aagacatgcc tgaacaatcc ggaggtggtg actatcccca ccacctcctt gaggagaccc
     1921 ccctcgacga cgaggacgct ccccttcccc tcgcgaacca tcatctggac cgcctcgaag
     1981 acggtggtct ccacccccac cgcgaggatg gggtaagtca tcagatcccg gacctttatg
     2041 agggctcccc ccctgccatt taagtaaaag ttgttttggg gcttatatta aacttatcag
     2101 ggtaaaggta ccggatatga ggccgcaagg ccgatttttg ggggcaggac ggcggccccc
     2161 cccgcccgag ccgccctccc ctctacgaac tcccgagagg ccagagccgg tcccacctca
     2221 actccagccc cacggcgggc tggtcgaggc cgaagctctg caggacctgg gggctgaaga
     2281 agccgaagga cccgaccttc gtcccctcga ccaggaggta ccccctccta cccgcgagga
     2341 acgcgccatc ctcggagggg actatctctg gcctcaggtc aagctcccgg aagagggcgt
     2401 ccaccaccga cctcacctcc gcgaggtcga cggagctgtg gatcgaggct gctgccagct
     2461 cctgtctcgt ccttcctcca ataagaacgt cccccagggc gaagatcctc tggggaaggg
     2521 gatggtgctg gtttatcgcg aagatctcga ggaggctcgg gaggatcgag gtcctgacca
     2581 tggtgtgaag ctcgctgatc gggtggagga cccgggtgac cgccccctca tcccgggggc
     2641 ggcccatcat ctcgaagttc accctctcag aggtgagggt gaagggcatc gtctccaggt
     2701 agcctaggcc gaccatgatc tcccggaggt cggactttcg gacctccaga gggtggggct
     2761 cgcctacggt gacggtcttg ggaaggaccg gcaccagccg gtcgtagccg taacccttgg
     2821 cggcgtcctc gaagatgtcc caggagtgca ttatgtccgc ccggtaggcg gggacgagga
     2881 cctccagctc ccggccgagg ggccggaccc caaacctcat cctctccagg gaggcggcca
     2941 gctcctccgc cgagaggtcg aagccgatga gggatcgggc ctcctcgacc tcgaccatcc
     3001 acctggcggg agcgaggtcg ggcaggacga gggtaccctc gggcctcttt acccggaccg
     3061 tctcgatcct gccgcccctc tccgccaggg cgcagaccac gatgttgagg gccttgtata
     3121 cgacgggatc ggtccccgtg acgtcgatga agaggtccct ggtatcgtct cggactcgag
     3181 tcagctcgcc gtttatgatc ggagggaagg agaggacatt gccgttggcg tcggtaatga
     3241 gggggtagcg gtcgaacccc tccaggatgg aggcgtagct cacccccttt gggtgacgct
     3301 tcaggatctc ctccatcgag aggctctcct cgaagtcgag ggggacgaac tcgaaggacg
     3361 ggtcagcggc gaagtaggtg aaggggggga cgacccggga gatgtcgtgg ac
//
LOCUS       DASM01000116            8453 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_237343, whole genome shotgun sequence.
ACCESSION   DASM01000116 DASM01000000
VERSION     DASM01000116.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8453)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8453)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8453
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_237343"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    4474..4508
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1724 a         2508 c         2365 g         1821 t
ORIGIN      
        1 ggaggcaagg caagcccttc cggttcctcg acttcaagaa cggcgagggg cgcgttatta
       61 ctggagagat gcccgacgaa aaggacgagc gggttgagga gcggctcgac tcccccgaga
      121 tgctggcggt gaacaccctc ggccagttcg caaagcaccc tcgggtcagc gccctccggc
      181 ggttcatcac cggctggtat gtctcctacc tcaccgccga cagcgttcga ggcattacgg
      241 aggccggacc tcaggaacgg ctctccatga acggggacaa cctcccgaac gtgatccagt
      301 acctcaaaga gcagcatcct gaccggctgg aggagatttt agccactcta tctcagagag
      361 tgccccggct ggagagggtt gattcggagt tcatgcccga cggtcggctc cttctggaga
      421 tcaaagacgc gcccttcgat aagccgatcc tggccaagtt cgcctccgac gggaccctca
      481 agatgctctc ctacctcacc atcctctacg atcccaatcc gcctcagctc gtggggatcg
      541 aggagccgga gaaccatctt cacccccgga tactgccgga gctggccgaa gaatgccgga
      601 acgcctcaag ccggacccag cttctcgtca ccacccactc ccccttcttc gtcaacggcc
      661 tcctccccga agaggtctgg gtcctcagcc gggacgagaa ggggtacacc caggcgaagc
      721 gggccgccga catgaggggg atcaaggagt tcatggagga gggggcgaag ctaggccacc
      781 tctgggtgga ggggttcttc gagatcggcg atcctattat tgaatcatca tccataagtt
      841 cacccgcttc agagccggga aagaaagccg ccgagtgatc tgtcatgcac atcgaatttc
      901 tggtcgaaga gccctcggca gaggcggctt tgagaaatct ggtgcccaag attctggggt
      961 cgggtgtcga gtttcgcgtc atccgtttcc agggcaagca ggacctcctc tcccggcttc
     1021 caggactttt gaagggctac aggagatgga tccccgccga ctggaggatt gtggtgctgg
     1081 tagacgaaga ccggcaggac tgccgtgagc tgaaagccga gctggagaag gcagcaggcg
     1141 atgcggagct gtccaccaga tcagtgacca gaggcggctc agcttttcaa gttctcaacc
     1201 gcatctccat cgaggagctg gaggcctggt tcttcggcga tgtggaggct cttcacctcg
     1261 cttatcccag gatcccacca agcctggcaa cgagttcgaa gtatcgggac ccagacgcca
     1321 tccagggagg aacctgggag gcgctggaga aagagcttca gagaaaagga tactttcacg
     1381 gtggccttgc caagatcgag gccgcaaagg ccatatccgc ccatatggac ccggatcaga
     1441 acagatcgaa gagctttcag gtcttcaggg acggcctgcg agatatggtg aattatcccc
     1501 gaagctgatc tgttccggtt tatgtgctca taatccctca gacgaactta tccgccctcc
     1561 tcgtcgcgag cggcagcggc gaatcccacc tcgtttagcg ccgccctagc ccttccctca
     1621 cacccgcccc cgccacctac cggaggctga tatgaagacg aggtttgcga cgacgcatat
     1681 cccgccggcg agaagggcgg tcagcaccgg cgacagctcc atcatctcgc cgatggcgct
     1741 tgcgaggatg acggcgatgg cgatggtcaa ggcgaacctg atgatgcgaa gggacccggt
     1801 ctcttccatg ggcagggcca tctcttctca tctgtaaaaa aggcttctgc gatcgatccg
     1861 tcttgggccg tcgaaccggg gaaaggtata agggcgatga tataaaaata agaagtccat
     1921 gcccctcaag atcctccagg tggacgcctt cacctcggtc cccttctctg gaaaccccgc
     1981 cgccgtctgc ctcctcgaag agcctcggga tgacgggtgg atgcagaagg tcgcagccga
     2041 gatgaacctc tcggagaccg cctttctgac ccccgagggg gacggcttcc gcctccgatg
     2101 gtttgcgccg aaggccgagg tcgacctctg cggccacgcc acccttgcat ccgcccacgt
     2161 cctctgggag gagggaattc tcgggccggg cgagaccgcg cggtttgcga ccagaagcgg
     2221 tcccatctcc gccgagagga ggggggagct gatcgagatg gactttccgg ctgagccgga
     2281 gagaccgatc aaatgctcgc ccccgatgct ggaggcagcc ctgggggcgc cgttctctta
     2341 cgtcggcgac aacaggttcg accttctggt ggagctcgac ggtgaagaga ccctccggga
     2401 gctgcggccg aacttcgacc tcctcaagaa gatcccggtc cggggggtga tggtgacggc
     2461 ggagagcgac tcccccgagt acgactttgt ctcccggttc ttcgccccga ccctaggggt
     2521 cgacgaggac ccggtcaccg gctctgccca ctgctgcctt gggccgtact gggcgaggag
     2581 gctcggcaaa ttcgaactcg tcggatacca ggcctcggag cggggcggcg tcgtccgggt
     2641 ccgggtcggc gaggacggcg atagggtcaa gatcgggggg gaggcggtca ccgtcctcaa
     2701 aggtgagctg atagagggat aggtcgaagc aaagctcaag ggtctctgcc gccttcctcc
     2761 atcaggcact tctccatcct cgtctcgggg gcccccgtct cctcgttgac cccttcgccc
     2821 gccgccttcc aacccctcgc ctgatagaac ctcatagccc ggaggttctc gctgaagcag
     2881 cagagcctcc ccgtccggtt tcctgcctcc ctcatctcct cctcgaggcg gccgaggagg
     2941 gcggtgccga tccccctccc gtggtggcga ggatggaccc agaggaggtc gacggcgtct
     3001 ccggaggcgg ccgccacccc caccacccct ccgccgccct cgcttccgac gaagctggcc
     3061 tctctatcgg ctagggccac catcatcctc ggccattctt cctcgacgag ccccgcgacg
     3121 acgtcgccct cgacccaggg gcgcaaggta tcggcgggga ggatggggcc gtagacctcc
     3181 gggatcgtct cgatgcagag gcggacgatg gcggagacgt cctctttcgc cgccctcctg
     3241 agagaaaact gggccatttt gatcggctcc tcttcgccga cccctccgtt aatatcttct
     3301 gccccgccct gaaagatgag atgcgaccga tgagaaccga agagagagga tacgggaaga
     3361 taatagaccc cacgatatcg agaggtgata tgacgatcta caaagcaaac cccggccagg
     3421 cgagctacgg cgaggcgatg ggcatcctcc ttctggacag ctttgccccc ttcatccccg
     3481 gagacgtcgc caacgctacc acctacgact ttcccgtccg gttcaagaag gtcccgggcc
     3541 tgaccacgaa gagggtcctg ggaaaagacc ccacagcctt tgagccgctc ctcgcggcgg
     3601 cggaggagct ggcggggcag ggggtgaggg ccgtcactgg ggattgtgga tttctcgccc
     3661 tccaccagag gcgccttgcc gctcaattgg agatccccgt cttcctatcc agcctcctcc
     3721 agatcccctt catatcgtcc atcatcggcc ccagccagag ggtggggatc atcgctgccg
     3781 actcgagggg gatcgacgag actttgcttt tagccgcagg ggtggagatg acggagaagc
     3841 tggtgattgg agggatggag gaccagagag ccttctccga ggcggtgatc gaggagaggg
     3901 ggatcctcga ctccgaagag atcgaagagg cgacggtgtc cgtcgcccga gagatggtcg
     3961 aagaggaccc ggcggtcggc gccctcctgc tggagtgcag ctgccttccc ccctacggga
     4021 ggtcggtcca ggaggccgtc ggcctcccgg tcttcgacta cgtcaccatg ataaactacg
     4081 tccactcggc cctcgtcaag aggaggtttg aggggttgat gtgaatcggg tttcagtcca
     4141 tcttcaaaag cttcgcattc acggcgacga tgacggtgct catgctcatc aaaaccgccc
     4201 ccaacgctgg gctcaagagg atcccgtagc tgtagaggac gcctgccgcc agaggtatgg
     4261 cgaaggcgtt gtaccccgtg gcccagagga ggttttggaa catcttggag taggtcttct
     4321 tggagagcct gatgatggtg acgacgtctc gggggtcgtt tcggaccagg attatgtctg
     4381 cggtctcgac ggcgacgtcg gtccccgcgc cgatggctat ccccacgtcg gcttgggcga
     4441 gggcaggagc atcgttgacg ccgtcgccca ccannnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnntt gtcgccggtg agcatcatgc acttcacccc catcgacttc agcctctcca
     4561 ccgcctcctt cgactcgggc ctcaccgagt cccctagggc gagggcgccc aagggcgtct
     4621 catcctccag gaggaagacg acggtcttcc ccccttcttc tgcggccctg accctctcat
     4681 catcgagctc gatccccgcc tcctccaggt agccggggct cacgaccttc agccttctcc
     4741 cgtcgacgac gcccaaaacg ccctttcccg gcatcgactg aaactcgtct gccggggcgg
     4801 tggagattcc ccgatcctcg gcgcccttca ccacgccccg ggcgatggaa tgctcggagc
     4861 tcgcctccag ggaggcggcg agcctcaaaa cctccatctc cgcttctccc accttcccca
     4921 cctcgccgac cccgacgagg gggattactt cggtgacccc aaactctccc cgggtgaggg
     4981 tgccggtctt gtcgaagacg accgcctcga tctctcttgc cctctcgaag gaccgcctct
     5041 ccctgatgag gaggccggat ttggcggcga gggaggtgga taccgccacc accaggggga
     5101 tggcgaggcc cagggcgtgg gggcaggtta tcaccatcac tgtgacgccc cgctcgatag
     5161 cgaagacgac gtcccttccg taatagatcc acgcggcgaa ggtgatcgcc cccaccgaga
     5221 gggcgatcac cgtcaggaag aaggcggccc tgttggccag gtcctgggtc ttcgacctgc
     5281 tctcctgggc agaggtgacc agggcgatca ctgtgctcag gtaggtctcc tttccggtct
     5341 ttccaacctc caccacgagg gacccttccc cgttcatcga tccgccgatc accctcgagc
     5401 cctccatctt tccgacgggc ttcgactcgc cggtgaggag ggactcgttg acgctgcttt
     5461 tgccctccag gacggtcccg tccacgggga ccttctctcc gggcctgacc atcaccctgt
     5521 ctcccttctt cagggaggcg atctccacct cctccacctg gccgtccctg agaaggtggg
     5581 cctcggaggg catcaccttc gccagctcct ccagggcccg ggaggctccc atcaccgacc
     5641 gcatctcaac ccagtggccg aggagcatga tgtcgaccag ggtcaccagc tcccagaaga
     5701 agaacttgcc cgccaggccg aagacgaccg cagcgctgta gaagtaggcg acggatatgg
     5761 ccaccccgat gagggtcatc atccccggcc ccctctcgga gatctccctc ttcaggccct
     5821 tcaggaaggg ccagccgccg tagaagtaga ccagggagga gagggcaaag aggaggtaag
     5881 aggagcccgg aaagaccagg tcgaagccga agaaggtctg gatcatgggg gatagggcca
     5941 agaccgggac tgtgatcaga agggagacga agaacctcct cttgaagtcc tggagcatgg
     6001 cagcgtggtg gtcccccttt ccctggccgc ctctgcctcc cttctcggga gttccgtggc
     6061 ctccgccacc gtgatcactg tggccttcct ctttgggagg tttatgatct ccgcctccat
     6121 gggccagatg gccttccttt gggcccccat ggcccgatgg catctcctct ccgaaggtgc
     6181 cgcccatctc tggagattta tcttcgctcg ccatgaccga tccccatatc taattgatta
     6241 ttatgactga tatattgttc gcttaagtcc tggtttgagg aagaaatgtc ggacaaaaaa
     6301 tggatgatgc cattgagatt gagaaaaata tttgtcggcg aagaaacgag ggaccgatgc
     6361 cacgtcttgt gccacctctg ttatgctccg ggtatgggat ctcgttttcg gattcgaggc
     6421 ccaggtcgtc gcctcctcca cgatcacgat cttctccgcc gataggctgc gttgctcgtg
     6481 gagcgaggcc gccgatccgc cggtctgcca tcgatccggc cgcggggcct tcttgggccg
     6541 cccggttgcc tcggccagcc gccagtttcg agcgagcata tcctctgcgg ggctatcgtc
     6601 gaaggagacg ggagattttc gccgtggaca gaatagatat attggattat ttaaatagat
     6661 tactaaatat aatcccggcg gcggaagacc gcctgcccgg ccgcgccccg ccccgccgat
     6721 ccgtgccctt ccgccggccg gcctgggact ggccctccca tccacctcct tcaattaacg
     6781 atcccggaga acaaccgccc ggcgcggaag cgccgggcgg cgccttcacc tcgcagcctc
     6841 ctcgacgatc tcgatctcct cctccgaggg gcattaacac tcggagatag ggaggcatcg
     6901 atccgctgat ccgacatcac ctttttacgc aattctacct gctagggatc ggctcattta
     6961 gagccgccat agctcactta agatctctat taaatgttct tgcctttttt caataacttc
     7021 gggagtccac tcttgttctt gcataacctg tgtggttaaa acgaaaggtg aaatcccttt
     7081 atctgtggcg aagtattgtc gttttttctt atcgaaatca tagtttcgtg cttggctgtt
     7141 tttcttgcga gataataata ccaaattgcc cagtttatga acgaacattt ctcgatcttc
     7201 tttttcggga aaatgtgtag tccaatagct tccctcgctg gggttttgcg gcattacatg
     7261 ctcaatagtt ataaatggat attcgtaaga tgcctttccg tcagatagcg cggcatctag
     7321 tctaagaagg acgtaccgag gtacattctt ggataaataa atatttccgt tcaatgcatt
     7381 taatatctca cttctctcct gaggtgtaag ttgcagagga gagcttgatt tattaagatc
     7441 atcttcagca tcgataaaag tcaacagctt accataccga tcaagacgtt ggttgatatt
     7501 tgcacgtaaa atcattaaac ttgctgcaag acgttctaat tctgtaaaga aatcaaataa
     7561 catatttggt ttatctttat tctttgagaa atagagaacc gccggaggta tccaatcaac
     7621 gttgtctatt cgattcaacc aagataatag agcgtttact tcctctgcat gtttatagct
     7681 ctggtatgat gagtttttga taacgtcgaa agcattggca tacgggatta atacattatc
     7741 tataaaattc ttaggatctt cggtaggatt tacataccgg cggaactctt caagtatcgt
     7801 atcttttggt ttagtttttc gatgaatcat tctgatatgg gcaaatagat cacgaaaagt
     7861 atctcttccc aaagctactt ctaaatcttc ccatttatta gtatatgaat cctgttcgct
     7921 taaaggtatt attttaccta tgacatccgc tttaataata tctgtaatag agagatctaa
     7981 accacgatca ttgagtattg agaaaatacg ccaggcagag ttgaaatcgg gggtggacac
     8041 tccgacaatt atgcaattgt ttatcacgaa ttgggtgagg cttaacaact ctttctcggg
     8101 ataatcttta agtttatcta ggaagtaaag tgcgttttcc actatgtttc gctggctatc
     8161 agaaagttta tttgtgtcta aattttttaa tttttctaaa ccgttttcgt cttgaatgta
     8221 ctccttgaag aattcggcat ctttctcctt tagtgttaaa cgataacggt tgggcgtgcc
     8281 ctttatctcg cttccttttt catatataaa aggagttata tcacttgcat acgttggtgt
     8341 taaatgccga agggtcgata gcagaattgt aagagtggcc aatcgctgct ggccgtcaac
     8401 cacttgagca tcaggagcgt aatctttgat cagaactatg cttcctagga agt
//
LOCUS       DASM01000117           13911 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_143134, whole genome shotgun sequence.
ACCESSION   DASM01000117 DASM01000000
VERSION     DASM01000117.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13911)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13911)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13911
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_143134"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2496 a         4534 c         4220 g         2657 t
ORIGIN      
        1 tcgccatagc cgtcgcgaac tcgatcctcc tctaccttta cgcccggaac ctgaaggtga
       61 cgcccttctt cggaaacctc gccgtcgggt acctcacggg atcgaccttc ctcttcggtg
      121 gggccgccgg cggggacgtc gggatcaccc tcttcctctt cctcctcgcg accctggcga
      181 ccctcgcccg ggaggtcgag aaggacatag aggacgtccc cggggatcga gccagcggcg
      241 cccggaccct cccgatcgtc atcggagagc ggcgggcgtc tcacctcgcc gcctccttcg
      301 tcctcatcgc gatcctcctc agctacctcg cccccctggg gagggcttac ctcgccgccg
      361 tcaccgtcgc tgacctccta ttcctcgcgg ctctggccag catattgagg ggggaggccc
      421 cccgggccca gaagatgctg aaggcgggga tggcggcggc cctcgcagca ttcatggtgg
      481 cggcggcctc ggggatgggc ctcctctgat gggaggaccc taccggataa ggccgagctg
      541 gcaggaggaa ctcgagtccc gccgtcgccc cgatcttgag acgaggagcc ccggccctaa
      601 aaaaggaagg gtcccctcgc cctcctccgg gggagcttcc tcctcctcgg ctcatcggcc
      661 ctcccccgcc ccctttccgc cttgagagga gggacctcgc caacgagtaa aatattggcg
      721 ccaggatggc gttagaggcg agggtgggga cccccgcctt caggagggac gcggcgacga
      781 agctgccgaa ggtgagcctc ccatcgacga tcgcctcgaa ggccccgcca cccctcgcca
      841 gatcaggaaa ggagaggatt ccgaagtagc tcgccaggac gaaggcggag aagctgacgg
      901 ccgtggcgac ggcgaccgcg gccgaggccg ccgccggccg gagggagcca tcatcacccc
      961 gccacctatc ggcggcgagc ttgcaggaga cgagccccgc ccagtgggcg gggacgttcg
     1021 ccagggggaa gggtgaggcg gtggcggccg tcagggcgag gcccgtcgca acgccgacca
     1081 cgttcgcctc cactaacgag atccgtaaga gaaggggcat gaggcagtat acggatatcg
     1141 cgatcccgaa ctggatatct ccggcgatcg gctccgtaaa ccccatcagt cgatgaacgg
     1201 tccacgatag ggcgatcagc ccgccgcaga gggctacctc cctggagttc atcctaaatg
     1261 gcctcgtcct caaatctccg gaaacctcac cggtgagcga agtaggcgac caccccgtcc
     1321 tccgagacgg agtcgatccg gacgaagccg tacctctcga actggacgac cttaccatcg
     1381 tcctcccgaa tgcctacctc gcctattccg gcgtcgacgc cgtcggggcg catcacccgg
     1441 acggggacgc cgtcgggggg ggcccagtga atgatccgaa gcttcatctc cttcgcctga
     1501 tgggggtcgg tccctatgac gcgggcccgg agggggtcgg tggcgatgaa ctcaaggttg
     1561 cagaggtctt tgagccggag cttccccccc tccttcaggt cggggaggtc gtcggcggag
     1621 atgaggaccc ggggccccgc ggggatctcg cgaaggccca ggtccagggt cgggtggaag
     1681 ggggcggtcg caacctcggg gacctcgccc tcaatctcca gcggtacggg gtccttgacg
     1741 aagaaccgcc tccttgccga tgggtcgacg atcctccggt tctcggcaaa gatcgagtcc
     1801 atgctgatgc tgatatccgt ctccccgacc ccgagctcga tcatgaactt tcggagcgct
     1861 tctgcgcgga tcccccgccg ccggagggcc cggaccgtcg gcatccgggc gtcgtcccag
     1921 cccgagtact cccccgactc cagggcccgc cggagggccg aggtgctgaa ggagccgaac
     1981 tggtggatct tgatcctccc ccagtggatc gtcgtcgggt acttccatcc catgtggtcg
     2041 tagaggtact tttgcctccg ctcgctgtcc atcaggtcct ttccccggag gatgtgggtc
     2101 acccccagaa ggtggtcctc gacggccgac tcgaagtcga ggaggggcca gacccggtac
     2161 ttatgccccg cccggggatg gtcggcggtg acgatccgaa aggcggccca gtcccggagg
     2221 gcggggtcct tgtgggcgat ctccgtcttc accctcagga ccgcctcccc cgggccgagg
     2281 gggccttcga tcatctcccg ccaaagggcg atgttctcct gggggctctg gctccggtga
     2341 gggcacgccg ccgccgcgtc tttcagagtt ttgaaggccg cctgttcgca ccggcagacg
     2401 taggcgcctc cattgcggat cagctcctct gccacctcgt agtagagagg gagccggtcg
     2461 ctagcgacaa ccacctcgtc ggggacggcg ccgagccacc tgcagtcctc caggtaccag
     2521 ccgtaggcct ccatcatcgg cctcttgttg acggggtcgg tgtcgtcgaa acggaggagg
     2581 aaccttccgc cgtaccttct gacgtactcg gagttgatga tgatcccccg ggctgagccg
     2641 atcgtaggcg gtccgttggg gttgggggcg aacctcatga cgaccccccc ctctgcgccc
     2701 tccagggaag agagcccctt ctccgggtcc ttcctcgccc ccagctccgc cagaagctcg
     2761 ggcgcgatct cggcgagccg cgcctcccac tcctgggggc cagtccccgc cacctccgag
     2821 aggaccgagg cgaccgtggg cgccacctcc ttcgccctcc cccggagctc ggggtgctcg
     2881 cccatcagct ttcccatcac cgcgcccgcc tggggcatag ccctgtactt cactgcgttc
     2941 tgaagggcgt acttctctat gatctccctg atatcgtcgg ccatgcaaga ccccctcctg
     3001 cacccgcaga tcttaaagct tctccagacc gtctctgacg atcagggcgt ggtcgaaggc
     3061 gaggggcggg agcgcttcga gggggaagac ctctgcagag gtcgcgtcgg agccggcccc
     3121 gagctccccc cgcccccagg cgaggtaaga gacggagacg acgtgccccc gggggtcccg
     3181 atcggggccg gagtagaccc ccaccagctt tatgaggagg atatccagcc ccgtctcctc
     3241 cctcgcctcc cgccggaccg cctcctcgac ggtctcgccc ggctccacga agcccccggg
     3301 gagggcgtag aacccctggt atggggggtt ctccctcctg atcaggacga tcccctcggc
     3361 gtagaggatg agggcgtcca ccgccagcaa tggcgtctcc atggggacca gaacgtattt
     3421 atacacttaa aggcagtggc ctaccgaaag atttatattc gacaaatgac gtaggctaga
     3481 ttggcaaaag tcgatggaat ttagtctccc tcctttaccc tacatccaat atcggctttt
     3541 gcgcccttct cttcaccttc tccagacaaa tccccactag agatatacat ggtgggacct
     3601 tgaggtgccc atgaacttct gcgtcatcaa gatcggcgga agcctgatct ccgtctccag
     3661 agatatcgtc agggggcttg gagccctatc gggggatggc gagcccttcc tcatcgtccc
     3721 cgggggaggc gccatggccg acctggtgag ggggctctac gatgaatacc atttatcgga
     3781 ggagacggcc cactggatgg cgatactggc gatggaggag tacggccggc ttctggcgga
     3841 cgggaccggc gccgccctca ccgcggagat atccaggccc gcctcggggg tctgggtcct
     3901 cctcccctac cggcccctgc tggagaacga ccgggagctc cgccacagct gggattacac
     3961 ctccgacgcc gtcgccgccc tggtggccag caggctcaag gtcgatctgg tcaaggcgac
     4021 ggacgtcgat gggatcttcc ttcacggccg gctcgccgac gaggtcctgg cggaggagct
     4081 catcgggacc gagacctgca tcgaccaggg atcccttcgg attttgatgg agtctgggag
     4141 gagctgcctc gtcctgaacg gcacggaccc ggaccggttc atcgctaagt tgatggcggg
     4201 agagggcgga acccttataa gagggagagg tgttggtcgg agcggtcctg acgtaaggga
     4261 aagtttatac cctgggatcg atcaaagcag gtagagagag gaacatgacg gaaaaatcag
     4321 agagatgtat gtcatgcgga gtgacgctga tcgggccggg tggcgccagg ttcccctgcc
     4381 ccaactgtgg ggagataatt attaggtgca acaagtgcag aaagcagagc aacccctaca
     4441 cctgtccaag ctgcgggttt ttgggtccgt gaggtgagag gatgggggaa gttgctgcca
     4501 agcttaaggt gatgcccgag ggcgccgacg ccgatatgga gaagatgaag gcaggaatca
     4561 aggccgccct tccagagggg gcgaggctcc acggcttcgc cgaggtgccc atagccttcg
     4621 gcctgaaggc cctgatggtc gccgtgatct tggcggaggg ggtcaccggg actgaggggc
     4681 tggaggcggc ggtggccggc gtcgacgggg tccagtcggt agaggtggaa gaggtcggac
     4741 tcctctgaaa acctccctct tcgccgtttt gatggggctc gtagatcagg ggtagatcgt
     4801 cacgtttgca acgtgaaggc cacgggttcn ngtagatcag gggtagatcg ctaccttcgc
     4861 aaggtagagg ccgcgggttc gaatcccgcc gagtccataa ggctcctatt ctcttcgata
     4921 tcggtattgg ccgtctccct cggtcggtcc ccttggtata caccagccca tcggtatacg
     4981 ccgtcccttg gcatcggcgg ttttgtcggt acaggctgtc gccttggtac ggcccttcct
     5041 atctattccc tcgatgggcg aggccatccg ccggttttgc cctccccatc tcctctgccg
     5101 tctcggcgat gaggtagcgg gcgtaccgat cttcagggtc gagggctgcc gcccgctcga
     5161 agcattcgaa ggcgtcgcgc cgcctcttca ggcggaggag gaggaagccc cggcccatcc
     5221 aggggccagc atcggcagga tcgatcgctg ccgcccggtc gaatgaggcg atggcctcca
     5281 tgtaccggcc catcatcccg tagacccggg ccgccctctt cgccgcggcg ttgccggggt
     5341 ccttctctgt agccctcaag taggccctca gggcgagatc gaggttcccc tcccggaggt
     5401 gtgagtcccc ctccaggatc agatcttcga ggtttatctt ccggtcgcgg ctgtccttcg
     5461 accggaggag ggacgaaaag gatgaaaaaa ggcgaccgag gccgatgagg ccccgggtca
     5521 tcccggggat cagggtttca gccgcctgat ctccaggtac ttcacctcgt ccccctcctg
     5581 gcgggcgaag accatctgct ttcggacgct atgggctagc ctgacggccc gggagacgac
     5641 ggggaggtgg aatatgtgcc cctcggggac ggagtggacg aggtactcgg agtgggggac
     5701 cttctttgaa ccctccaccc tcttgtagac ccggaagtgg cttccgaact tgaacccggt
     5761 cttcaccacc agcctccggt ccctgaggtc ccgatagacc agcctcttca tatcgaactc
     5821 ctcctcaacg agcctagacc gcctcgaaaa ctcctcgggg gtgagggggg ctccagaccg
     5881 atcgacgatc ctgaggaccc ccttctccag gaggtacgcc gactccacca gggagagctg
     5941 gagcctcccg cccacgggct tgccgtagaa ccccctttcg tggagggcct cggaggcctt
     6001 ggggtcccag agcatcaccc gatcttccag gagggtggcg gtgccgcccg gggcctcgac
     6061 ctccatctcg cccttcaaat ccctatcctt cgcctcgtag aaggttatgt cgctctcctc
     6121 gtcgacgacc gccagcatca gcctctttct catctgcctt gcgatctttg ccggctccac
     6181 cagctctttc agggggacgg gctccctctc cgagacgacg tggacgtaga actcggcggg
     6241 ggtcttcccg ggcttccccc ctcgggggta gacccggaag tcggtgaccc caggctggac
     6301 gtagtagccc ctctcccgga ggtccttgta gacgacgtat cggaactcaa agcccgcctc
     6361 cagggccgtc gccgcctcaa agaaggactg gaagtcgaga tcctctccgc cgacgcggac
     6421 ggatatccga ccccggtcca gaaggtaggc tgcctccacc agggagaggt ggaggccccc
     6481 ggggacgggc ctcccgaagc tcccctgctc gtagagctcc cccgtcgccg ctccgcccag
     6541 gaggacctta tcccccgaca gctcgcccag gatggggtct tcggccatga tctgttgggt
     6601 ctcaggtgga gaggctgtca ggccagacgc cgtggacgca tgaaagggcc tcgtctacgg
     6661 tgcggtggac cttctctgcc ggatatccga cgctgaggag gtaggactcg acgtggttgg
     6721 ggacggcgat ggacatctgc tcgaccatca agaccgtctg gaggatggtg tagtcgccga
     6781 gcttgacggg gattatggtg tcggagacga ggagctcgaa gatctctggg ctgatgggcc
     6841 gctcgaaccc ggagaggaag gcctccatga tctcgctcgt ccactcgtgc ttctttcctc
     6901 cctcgtcctt gtaggggatc accacgacct ccctcgtctt cgctaggctc tctacgaagt
     6961 cttcgggagc gacgtcgccc tcgaagacga actcgaggaa gctggtgtgg gggaccgtcg
     7021 tcgggatctt ggaagccctg atgctgtagg agacctcctc gaagaccgtc cccgcgtccg
     7081 tcccctggtg gccgacgccc ttgccgatgg agacggcgga gggggatgcc cggacgacgg
     7141 tgtgggggtc gccgtgcctt cgatcgatgt ttccgaggac catcttgagg ccgagggggc
     7201 gggcggcgag ggatgccctg cagatccccg tggtgttgca actggtgcac tggatcagcc
     7261 tcggcttcgc cttagagaag gcctcatagt tctcaaggcc gaagaagaac tccctcttga
     7321 agtccgcctc gaggtcgccg gagatctcga cgcccttctc ccggagagcc gccattatct
     7381 tatcccggtg ggctcctccc atgagggata tggtgcagcc ggcggcgaga cccgcctcgg
     7441 cgtagggcgt ctcctgatcc ccgggggtcc cgatcatgat gaagtcggag tcctcgaaga
     7501 agtcagcgta tcctccctgg accgagaagc ccgccctctt ccacttcgcc ccatcggcgt
     7561 cgtcggagac gtaggctttg acgtcacaga tcctgatcag cttgctgagg atgctcttag
     7621 ccctgccgtg gggctggcgg aggaggacgt tcagatctcc cccgatcccg gcggccttct
     7681 tgaccgcgtc cagggcgagc acggttcgct tattctccgt cccgtcgaat ccgacgaagc
     7741 ctgcattgga tttcaatctc attcctcctg ggttgatgaa agaagtgtcc cgatataacc
     7801 tttcctgctg aggtccctct tcgacatcct gctgggggag gccttcaaga accctcgaag
     7861 gtctccatga tccaggctac gaccttatcc gcgttctgat ccgggtccag accctcggcg
     7921 tccaccacca cctcggggct cttcggctcc tcgtaggcta cgttcacccc cggcaccgtc
     7981 gccccatcat ccaccgcctt cgcgtagatc cccctggggg agtagaccgc cttccgcccc
     8041 cgctcccgct cgatgcaggc ggggagggag gccttgacgt aaacctcagc gaaagtgggg
     8101 actagcatcc gcgccaggtc ccggtacctc ctgcggttgg cggtagcatc gacgatgacg
     8161 ttcgtccccg cctccgagag gagcttggcc atgtaggcga ggctggcgta gaccacctcc
     8221 ctctcgagat cggtgtaagt cggctcgggg gtgatgaccc ggcggatctc gtccagctcc
     8281 aggaccttca ccctgatccc ccgcccttcg agcctggcct tgaccccccg ggcaagggtg
     8341 gtctttccgc aaccgggaag acccgtgaac cagatgaccc aggacatgga gaaagagacg
     8401 cccccccgcg aataaatatc attcccccgg gttcgaacaa gtttgagggg atggtcgcgt
     8461 tccggagccg atatcggtcc atcagacccc agaggatcgt cctgagaagg caaatacttc
     8521 gaagaaaata ttcgatcgaa caaaagatta atataagtgt tctcgatata tagagatgca
     8581 acgggacata tccatctctg catagggata atcagaggta taaaaatatg accaataann
     8641 agccgagaca ggcggctttg aaggctgcaa accgcggata taccgacatt aggctgcggg
     8701 agaggggcac aaagaaggtc cacctcttca agggcgagag gaagctggtg gtgaagccct
     8761 ccaacgcccc cgactggatg cccaaggaga tctggaagcc gaacgtcaag aaggtgggga
     8821 tagagaagct ggaccagatc tgaggatctc ttccctccag ggggcggcag gcgggagacc
     8881 gcccgccgcc gtcttatcct ccaaattggc cgtccaggcc ggcggtgcag ctcacttttt
     8941 taggcgattt ggctcgatta tcatccatct cctcgggcct cgtctacgtc gaggctgagg
     9001 aggagcctca cctccctccc ctgatggtcg acggtggtcc ataggacctc cctcgccgtc
     9061 ccggatatct cgtactttcc ggtccccgac ctctccacct ccacctttcg ggccaccgcc
     9121 agaagctggg tgaactcggc gaagaggggg tcgatgaagg tgttcctacc gatctgctct
     9181 ccgtccacat catagactat catcccgtcg gggagcatcg cccagatctt ggcgtggcct
     9241 tcgggctggt ggggggcgag ggcctcgccg aagagctccg tcgcgttgac gaggaccgtc
     9301 accgccccca tcaacctgcc attctcatcg aagaccggcg acgcgaagtc catggcgggg
     9361 atcccctcga cggagatgat gtaagcgagc cccgcgggtc gccgggtggc caaaagctcc
     9421 tggatatgct cctggtcgtc tatcctctcg cccaccacat acctgtactc ctccggcgcc
     9481 acctccagga tcgtgccgtt gaggtcgatg gttatgcagt cgacgaccca gggtccgact
     9541 ccaccaagct cctccagaac ccttcgggcc tccgccccca caatccccgt ctcggagagg
     9601 gcgaggctcg cccccaggag ggccctgtcg atctccagaa gctccccgcc gacggcctcg
     9661 gccgcctgga cgaggttcca gaccggctct cccccgacaa aagattcatc cgaagccgcc
     9721 tcccctgcga tggcggcggg gaggaggagg gccaagacca tcccgactcc agataacttc
     9781 ctcatcttct cagaggatat acccatgaaa gtcctattct tcgatggcat catagcatca
     9841 ccagattacg gaaggccatc tcctccgaaa ggatataccc ttcccctccg agggggcgcc
     9901 agggctgaag atcggccccg aggagccgga tgaaaaaaag agaaggagag agggctcagc
     9961 cgaagagggc tccgaggccc tcgataccgc tctcctcagc ctcttcctta tcttcctccg
    10021 cctcctcggc ggctgcgggg gcggccgcgg gggcggccgc ggcgggcgcg gcggccacgg
    10081 cgacgctggg ggccttggag aggacgtcct cgatgtctac gccctcgagg gcggagacga
    10141 gggccttgat ccttacctcg tcggcggcga cgcctgcggc gctgagtata gtctttatgc
    10201 cctcttcgga cacgtcttta ccagagctgt gaagcaataa tgctgcatat acgtattcca
    10261 tctttatcca cctcttcgtt ccgatcaacc aaacagggca gaaagtccgc caagtccggc
    10321 ctcttcattc tcttccttct cggcctcttc ggccttctcc tcttccttct ccgccggggc
    10381 tccagcggcc ggcgcctccg ccggcccgcc ttcgaccagg ttcgtcagga ctgacgcatc
    10441 ggcagccgcc ctcgccagga gggcctccat catgccggga acggggatgg cgcactccag
    10501 gaccagattc ctggccttag aggcagccct ctggaggagg ggagcgatcg tagtcggcgt
    10561 cgcataccct atctccaggg cgaggcccag ggcctgggat gatgcggtgc tcatctgaga
    10621 gaggatgcta tcgacgtcga tggtgagatc taccgcctta tagacgaggc ctccctcgta
    10681 gacggccaga agctcaagcc ccaccttcct cgggaatatc tccatcagcg agagaacttc
    10741 tgcgagctgg gccgataccg tctcgccctc ttcgacgacc accttcgtct cgttgatgac
    10801 caccttgccg ctctttatgg cggcggggat gccagccgcc tggagcttcc ccaccatggg
    10861 gccgggggag aaggaggtct cacccttctc gatgacgatg tctttggggg ccttgccgcc
    10921 agcctttatc ggcatcggct gcttcttctc ctccagcatc ttacagagcc tgaaggggtt
    10981 tacgtcggtg aatatgaacg ccgtctgacg gtctatcccg tcggccaggg ggaagacctt
    11041 ctcggagcac ttctctatcg cccggcgggc gatgttgttc ctgaccatct ttatggtggc
    11101 cacatccctc agctccgacc gcagatcctg gagctgcttt gccgggatct ccgcgaggcc
    11161 caccaggccc acgacttggg tgctgtttat tctctcgacc agctcctcga cctcttcgat
    11221 cttccacttt gggacgtgct ctgtatggcg tacctggacg gacatttcag atcaccttta
    11281 tcgctggacc catggtagtc tttatgtata cggacttcag gttctgcttt ccgtccttga
    11341 gagcctgctc gagcttggtc gtgaccgcct ccacgttctc tgcgatcttc tggacttcca
    11401 tctccctgga gccgacggcc atgtggaagg tgagccggtc ccgggatctg accctcacca
    11461 ggttcctctg cctcttgatt atgggcgtga tgtccacgtt ggggggcagg ggcaggggca
    11521 tcttccccct ctttcccagg atggggccca gctttttgcc gatcgtcggc atcaggctcg
    11581 tctcggcgat gaagtaggtg tactcatcgg cgatcttccg cgcccgcttc ttgtcgtcgc
    11641 ccagctcacc gagctcgtct ggagatatca ccagatccgc cccagcccgc tgagccataa
    11701 gggcggtctc gccgctggcg aagaccgcga ccttggcaga tctgccccgg ccgtggggga
    11761 ggatgatctc ctcgtccacc cggttggccg gctggctcat gtcgatattg tgcaggttga
    11821 tcgccagatc gacgctctca ctgaacctcc tctcgggggc ctcggagatc gcctttctga
    11881 cggcgtcttc tatcgtcttg ttcatctcaa tcctccgtag tccgcgactt gcagtctact
    11941 acggcatctc aaaaagagag tccagattcc gtctgcaatc ctctataata acttttcgtc
    12001 tatgcgagga tctcgtcgaa ccttccctcg tcaaccgccg cctgggcctc cttccggggc
    12061 ataccctcta cggtgatgcc catgctcccg caggtcccga tgacctcctt cgcggcgctc
    12121 ttcatggaga cggaaagcag gttcttcctt ttgatccctg cgaccttgac cacctgttcc
    12181 atggtcaggt tgccgacgat ctggagagcg ttgccggatc ccttctccag ccccacctct
    12241 ttgaggacca aggccgaggt cggaggggtt cccacttcca cctggtaatc cgtcctgctc
    12301 ctcaccgtaa ccttgaccgg gacctgcatc ccgttcaaat ccgccgtagc ctcatttatc
    12361 ttcttcacca catctgccac gttgacccct agaggtccca gggcgggtcc caggggtggt
    12421 cctgcgctgg cctttccacc ggggaccaat acttcaacaa aactggccaa gaacttcacc
    12481 tctgaatctc atctttgctg aggacgcgga cgtggtcgcc cctgacagtt atggggatgg
    12541 ggaccatcgc ctcgaagagc tctacagtga tctcctcctt cccgatgtcc accctcttga
    12601 ccctcgccat ctcgcccttg aaaggtcccg agatcagctc cacaatggcg ccttcgttga
    12661 tcccgaccac cgcgggcttg ggcgtcagaa agtgctccac ctctccgaag ctggaggctc
    12721 cctttatgac agagcgggcg tggggtaccc cctggatcgc ctcctcgacg atctccgggg
    12781 ccttcgcctc cacgaggacg tagcccttca ggacgtccgg aaccagaagc gccgtaatgt
    12841 cgtacttctc cttccgcgcc agctgggcga tgaggtttgc cacggaccgt tcctgatttg
    12901 ctgtggtctt aactacgaat atgctggtct cggccattct gacgaaatga cagtcgaacc
    12961 ggctatatat aggttttcag ccagcaccga tacagccgaa tgctcgttat cgaatccgag
    13021 gaggcggggg ggagggccgc cgcgacgaag ccgcccccgc caccattgta ccgcctaccc
    13081 cgggggccgg aatcccgaaa ggttcatata gaagtttaaa cacctcgcaa gccatcagat
    13141 caactggagg aagaaaaaag atggcaggac tagggggaac ccctgtactc atactgagag
    13201 agggcagtca gaggactgcc ggccgtgagg cccagaagtc gaacatcatg gccgccaagg
    13261 ccgtggccac cgccgtcagg accaccctcg gccccaaggg aatggacaag atgctcgtcg
    13321 acaccctcgg cgacgtggtc ataaccaacg atggcgtcac catcctcaag gagatggaca
    13381 tcgagcaccc agcggcgaag atgatggtcg agatcgccaa gacccaggac gacgaggtcg
    13441 gcgacggcac caccaccgcc gtggtcctgg cgggagagct cctcaagaag gcggaagagc
    13501 tccttgacca ggagattcac cccaccgtca tcgccgcagg ataccgggcc gccgccgaga
    13561 gggcgatggc catcctgaag gagatggcga tcaaagtctc ccccaacgac gacgagcttc
    13621 tgaagaagat cgccatcacc gccatgaccg ggaagggctc tggcgtcgct cgaaacgagc
    13681 tggcggagct ctccgtaaag gccgtcaagg ccatcgtcga cgaggacggg accgtggacg
    13741 tcgataacat caccgtcgag aagaaggtcg gcggcgggat caccgactct cagctcgtct
    13801 acggcatggt catcgacaag gagaggctcc accccaacat gcccaagaag gtgaaggacg
    13861 ccaagatcgc cctgctgaac actgcgatcg agatcgagaa gaccgaggtc g
//
LOCUS       DASM01000118           10652 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_259523, whole genome shotgun sequence.
ACCESSION   DASM01000118 DASM01000000
VERSION     DASM01000118.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_259523"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2347 a         2986 c         3137 g         2182 t
ORIGIN      
        1 agaacctggt gagggggacc gaccctgggg cgccggagtc ggtccacctc cacgactggc
       61 accgcccccg ccacgacctc atcgacgaag agctggagag ggagatgggg ctggtccggg
      121 aggtcgtcga ggcggtctcc aacgcccggc agaagggagg ccggaagctg aggtggccgg
      181 tctcggaggt cgtcatctcc ccccagcggg agatcccgga cctgaaggac ctcctggggg
      241 tcctcaagag ccagaccaac tccaaggagg tgacggtgat ggccgtcggc gtaaagcccc
      301 ccatggaggt cgccctaaac cctgtcccca agaagatcgg ccccgccttc aagggcgacg
      361 cccagaaggt gatcgccgcc ctcaaggagg cggacccggc gatggtgaag gcggggatcg
      421 aggccgggga gggcaaggtg ggtgcctacg tcatcctccc cgagatggtg gagttcatag
      481 agaaggtccc cgagaacctg gtggcggcgg agttctccag cgggaccgtc tacgtcgacg
      541 tcaccctcac cccggagctg aaggccgagg gttacgggag ggaggtgatc aggaggatcc
      601 aggagatgag aaaggagctg gacctccggg tcgatgagaa gatcgtggcc gccgtcaggg
      661 tggaggacga ggaggttctc cggctcgtcg aggcgatgag aggccacatc gcaggggagg
      721 tgagggctcg acggctggag ctcggctcgg acctggaggt ccggggctct cttgtcaagg
      781 aatggtcggt agaggacgtg gccatgacca tgggggtcac ccgcagcagt taatatataa
      841 gatgagtggg agaggggatg gcatggaaga gccgtcgcct ctcctgaagg tgagggtgaa
      901 gggggtctac gccctccatg ccggcgccga cgtggtcgcc cccgtcgtcc tcctggagga
      961 cgaggcctcg aggatcgttc ccgtcttcgt cggcctatcg gaggcgatct ccatatattc
     1021 ggccttatcc ggggcggtct ctcctcggcc caccacccac gacctcttca tctccaccct
     1081 ggagagcctc ggggcgagga tcgccggggt ggtcatcgac gacctggagg gcggagtata
     1141 ctatgcccgc ctatccgtct ccatcgactc cggcgtccgg gaggtggacg ccaggcccag
     1201 cgacgggatg gccctcgccc tccgggccaa cgcccccatc gccatccagg agagggtggc
     1261 ggtccaatcc gccatggaga ggaaggacct ggagaagttg aaggggatcg agaactatct
     1321 gtaaccctcc ccgggcagat cggagaggca tgaagaggcc ggcataaaac cacctttttt
     1381 ttagcccccg ccggtgtgat cgaagggagg atggcgattt gtttagccat tttaattctc
     1441 taataaaaat catgggaaaa cgtttaatac catccgccga gaagatttaa tggtcctctc
     1501 caggagagga ccttttggac aatgggcaaa ataaagatgg gttagcacgt ccagccttga
     1561 aatactggcg aagatgaaga tcgcgaccta gagtgaaggg ggtacatctt gccgagggat
     1621 aagggagggg atcgtgtatg gccgtatttg agggaagacc atgaggcgtc aatcaaccat
     1681 ctgcctgacc tgctttctgc tggtctgtag ctttagcatt cttccattct tctcatccgc
     1741 cctggcccag gggatcggcc agggagagac cgtcataaga tggcagtacc gcccggaaga
     1801 gccgatcatc caggtgggcc tcgggggaga gatcctcccg atgggggctc agaacctgac
     1861 ccaatcgatc aggataccag gccttgagaa caggatcatc cccggagagc cggtggtccc
     1921 cttcagaacg gcgaggatac tcgtcccctt cggggaggag atccaggaga taaaggtcct
     1981 gccgggggag aagaagaacc tgggcaagat cctgatcgag cccggccagg accccctccc
     2041 catcagcttc tcggggaacc ggaccgtcac ccccctaaac gagagcacct actcctccct
     2101 ggagccgtac cccagagacg tcttctccgt cgcgggggtc caggagaaac agggctacaa
     2161 gatcctattg attaaccttt atccgatcat gtacatcccc aagacgaagg acacctacta
     2221 cttcggcacc ttcgacgtgg aggtgaagac ggctcccctc gggaccctcg atcgggggct
     2281 cttcaggggt tctgaggcgg acggagctca tatagcccgg ctcgtcgaaa accctgccga
     2341 ggtcgggtcc tacgccagcg atctcgccgc tatgggggcc actcccctcc ttgatggtga
     2401 ttatgattat ctgataatta cgaatcgggc tctcaagaac gcccccgtcc cctacaactt
     2461 cacagacctc gtccgttgga agagctcgaa gggggtcgcc tacaccatcg tcaccgttga
     2521 ggatatctac tctgcatata ccggcgccga tgagcaggag aagatcagga acttcataag
     2581 ggatgcttac ctctaccacg gcatcaccta cgtcctcctg gggggggatg ccgacggagg
     2641 caggaaggga ggagagggct ggaacagcat cgtcccctcc aggggcctct gggcctggga
     2701 ctacgaggcg actcccccga tgatcccctc ggacctgtat tacgcctgcc tcgacggaga
     2761 ctacgactcc aacggaaacg gcatctacgg cgagccggaa gacggcccgg gcggtggtga
     2821 ggtggatctg atggccgagg tctacgtcgg gagggccccc atcgactctt acgccgagct
     2881 ctccaacttc gtccgaaaga cgatcgccta cgagtccgtc tccaccgacc cgtacctgga
     2941 gaaggtcctg ttggtcggcg agtatctcgg cttcggcggc gtcagcgact ggggaggtaa
     3001 ctgcctcgac gagatcaaag atgggtccgg gtcgagcggt tacaacacca cggggttccc
     3061 cgaagactat cagaagtcca ccctttacga tcgcgatcgg aaatgggata aggccgagct
     3121 gatggagctg atcgacggcg acgtccactc cttgaaccat ctcggccacg ccagcgtcgg
     3181 aagcgccatg aagatgaacg tcgaggacgt cggccgcctc aagaacgacc gccccttctt
     3241 cggctacagc cagggatgct acgccgggtc cttcgacaat cgggaccccg actccgtcta
     3301 ctcccccacc gactccatcc tggagcattt tgtaacggag ccccacggcg ctttcgcctt
     3361 cataggcaac tcgcggttcg gctggggtag gagggagacg acggacggcc cctcccagag
     3421 ataccaccgc cagttctggg acgccgtctt cggagagggg atcagaaaca tcggccgggc
     3481 cctccaggac gccaaggagg ataacctggg gtccatcgac aagacccccg atgggaacgt
     3541 aatgaggttc tgctattacg agatcaacct cctcggcgat cccgagacct ctatacacca
     3601 cccctttacc gctagccaca acctggaggt cgtcgggatg gacctcccca gctacaccaa
     3661 gcccgataag cccatagagg tcagggctgt cgtcaagaac agggggatag aagacgagtg
     3721 gaacgtcacc gtccagttgg ttgaggtgga tgcggtccgg gactcggagg ttgtgcctca
     3781 ccttcccagc ggcggatcgg ccgaggtgag gctctcgtgg tcgggggcgg cggagggtag
     3841 gtacatcctg gaggctaggg ttcttcccca ggagggcgag gagctgactg aagacaactg
     3901 gcaggagagg gaggtagagg tctatagcaa ccggaccctc atcctcctgg tggacgatga
     3961 cgagggggcg gagtacgaga gctattacga agaggcgctg gccgccagcg gatttgctta
     4021 tgccaaggtg gagcggccgg ttggggagag cgagcttctc tccttcgact gcgtgatatg
     4081 gttgaccggc agagacttca ccaccgccct caccggagag gaacagagga ccctcgcccg
     4141 gttcctcgat gccggtggga gcctcttcct ctcaggccag gatatcggct atcacagcca
     4201 tgcctccgac ttctaccggg attacctcca ggtggagtac gtaaaggaca gcaccgggat
     4261 ctacgtcctg gaaggggtgg aaggcgattc catatccgac gggatgacga tcgagatctc
     4321 cggcggcgat ggggcggaca gccagtactg gccgagcgag atcatgccga tcaccgccta
     4381 cgccaccggg atcttccgat ataatgacgg gggctggggg gcggtgaggg tggatacggg
     4441 gacctacagg gtcgtctact tcgccttcgg gttcgaggca ataaacagct ccgcggagag
     4501 ggctgagatc atgaggaggg tcttatcgga gctgaccggg cccgagacaa cctccgtccc
     4561 cgaatatggg atggacggca ataatctcag ctcggaggag ccatcccccc tgaggaggag
     4621 aggcggcccg aaggatggca tgggggaggt atttgatatg gatctcgcct tcaaggagat
     4681 gagccaggag ttcaaagagc tcggagaagt caatcaggtc tttgagggct caatctttga
     4741 gttcggggat atgggatcga ggttaggggg cgaaaaggga tatgtttgaa gattggatca
     4801 gggccgggtc tgggcagaag ctcctttctg ctataatcct ggcggcgtta tctatagctt
     4861 cggctcaggc tgccgccatc accgtcggcc ccgaggggtg cgactatgac agcatccagg
     4921 gggcggtcga ggcggcagaa ccgggggaca ccatcaccgt cgaaggcggt acgtataagg
     4981 agaacgtcat cgtcgataaa agcctcgtcc tccgggggaa ggggtcaggg gaaggaaggc
     5041 cggtaatcga tggaaatggg gtaggtagcg ccgtactcct cgccgccgac cggatcaccc
     5101 tggaggggtt cgtcgtcaaa aacgccggct tcggcaaggc ggggatcgag gtgaagtccg
     5161 ataagaactt cataagaaat aacctcgtca ccgccaaccg atggtacggg atctccctat
     5221 ctgattccca agagaacgtc ataaccggaa atgtcgtcag cagcaacaag tacgggatct
     5281 ggatctcttc ggggtctagc agcagcagga tagcccaaaa ccagctggag aagaacgcca
     5341 acggcaacgc tgtagacgcc ggaagaaatg actgggaggg gaacgcctac gacgacctgg
     5401 aggaggggga gacgagatac aggatagccg gaggatcgaa cgtcgacgag agccccaagg
     5461 cattagcacc aggatcgggg aacgatgagc ccaagacctc gacgataacg gtgaccatca
     5521 ccataccgac tccagtcatt acctcctaga actcggcggg agcgcaaaga acccttctag
     5581 cctaagatca aatacccctc cccgatgccc ggggcccgca caccttttta tactcccccc
     5641 gacaagagct tcgaccctga gctggagatg atacgaagga tgcttactgt gatctttctc
     5701 gggacggcgg gctcgctacc cacccccgag aggagcccat cggcggtcct gatcaaccgg
     5761 gagggggaac tcctcctctt cgactgtgga gaggggaccc agcggcagat gatgcgggcg
     5821 aagacgggga tgatgagcct cgacaccatc ttcataaccc accatcatgc tgaccacatc
     5881 ctgggaatac cggggctctt ggagacgatg gccttccagg gtagacgaga tcccgtcacc
     5941 attgcaggcc ccccccggac ctctgaggtg gtggagctct tcggccgact ctgctactac
     6001 tctcggaagt ttccggttag ggccctggag ctggagccgg gggacgtggt gaggagggag
     6061 ggctacgaga tcgaggccgt caggacgagc cacagcgtct ccagcctcgg ctactgcctc
     6121 cgggaggatc cgaggcccgg gaggttcaac cgggagaggg catccgccct ggggataccc
     6181 cccggacctc tctttgggag gcttcagcgg ggcggggcgg tggaggtggg ggggaggacg
     6241 gtgcgacccg aggaggtgat gggccctccc aggcccggta ggaagatagt ctacaccggt
     6301 gacacccggc ccaccccgga ggtggaggag gcgagcaggg gggcggacct cctcatccac
     6361 gacggctccc tcgacgacgg tatggcggat tgggccgccg agacgatgca caccaccgcc
     6421 ggggaggccg cccgcctagc cgagagggcg ggagcgagga ggctcgtctt gacccacatc
     6481 agctcccggt actctgacga cgtcggcccc ctcctcgagg acgcccggaa gcactttccc
     6541 tccgtccaga tagccgaaga cctcttgaag atagaggtca agctgagaga cctctgagcc
     6601 ggggccggga tcagggatga tctcaggatc tctccctatc ctcggcggct cgcctaatcg
     6661 tcttctcccg ctcctcgacc atcgcccttc tgagcccctc ggcgaggtcg tcatccctgc
     6721 cgatgagggc gttttccagg gatagggcga tggtctccag ggcgtagccg acccggccca
     6781 ggtccatgct cctcctctcg tggttcaccc ccgcctcgaa gagccgctcc agctggtggc
     6841 tggggaggcc cagggcctgg ccggcgctga cggccctgcc gtacatcttc tcggagacgt
     6901 aataatcgag cccctcccgg tagagcctca tcgactcttc ctccaggccg gccttcttgg
     6961 ctatgagggc agcccatagg tagtcccctt cctccagggc gatctccatc gccttatccg
     7021 tccgtcctga gtccttggcc agggatatgg cgagctccgg ctcgccggcg ccgatcaata
     7081 tctcgatccc cggctctatg agctcctcgg ggagctgcct taggttctct gtgaggtaga
     7141 ggacctggtc ttcccggctc agtttatcga actcttcact agagattccg atcaaagcca
     7201 tcaccttaat ggttcaccca aggagggcgg ttatcttcga gaagtcgacc ctggcggtgt
     7261 tgtctatcgt cagaggggtg atggagatct tcccctcctg gtagacggcc cggacgtccg
     7321 tcccctctcg ctcggtgcag acgaggtctc cgtcgatcca gtagtagggc ctgcccctgg
     7381 ggtcggcccg ctcctggatg gcggtcctga agatcttccg ggctagccgc gtcaccacca
     7441 cctccgtctc ctcggtggcg ttgacgggca cattgatgtt caggatgtcg acccccgggg
     7501 ggagccccct ctccaggacg tttttggcca cccgccggac gaggtggctt gcgacgtcaa
     7561 actggtaggc gtgatcgttt atgctgtcga acttgtcccc ctggtcgatg acctggatag
     7621 aggctgttat cgccggtatt ccgtagcttg ccgcctccag agctgcgccg atcgtcccgc
     7681 tggtggtgac ggtgtcggtg ctgatgttct ctccgatgtt gatccccgat actgcgaggt
     7741 caggaagctt ccccttcagg agccagaata tccccatgat gacggagtcg gtgggggtcc
     7801 cggagacctc gtaagcttcg accccgtcca ggttgacctt gttgtacctt aggggctcga
     7861 agatcgatat cgaccttccg acgccggacc tctggccgga gggggcggcc acgagaaggt
     7921 cgccgaggcc caccaccccc cggaccgccg ccctgatccc tgcggagtag acgccatcgt
     7981 cgttggtaat cagaatacgg tgcatctcta ccttatcagg ggatggaggt ataaaaataa
     8041 agcgacgtcg ccgacggatg aatgtattca agggctggcg gggtaaggat ccacgaagaa
     8101 gatggactct tccttcaaac ggcaaaagga gggcggacgt cggcgcggtc agcgatggcg
     8161 ctatttttag ccccggttcc ttaaatcatc cacctaccgc cgaaaaggcc ggctcaaaag
     8221 gggatcaggc ctctcggtcg agtgatccat ccatctcctc caggctatga actctcactt
     8281 ttcaggttca aatttaacaa tgatttatta aatataagaa taaaactcta tttttataaa
     8341 ttatatctat gtatttaaaa ttaagaattc tattgtagtt taaacaataa atatactaat
     8401 ttatatattt tattttatga gatatatgtt taatcatagt ctatattcga aattttatta
     8461 aaatttgact tttttttgag cggacagact tgtatattaa taaattatat ataatgaata
     8521 gcctaaaatc tagatctccg tcctcctcca atcgtcactt tgtttcgtaa ataacttgaa
     8581 tagatttatt aattcagaga tttttataaa catctaatcg gggcataaaa tatagacttt
     8641 atgtccttta aaatcattta taaaattatc tacataatac tttccacccc tcaatcattc
     8701 ttatactata tcggcatgaa ctacctctcc aacacctcct tcctttcaag ggcctctttc
     8761 ccctcgcgaa gctccatggt atgcgacgag ggggccatat tcatataacc tttggaacta
     8821 acgaattaca gagatggccc atctttggag atctaaaata ttgtgcttat aagttatgtg
     8881 aatgaggatc tggggaggaa ggcatcctct ttagtttgga gcccaactcc catcccacct
     8941 ctgcatcgaa gatgattgaa atatttagat ttctttatac tattaatcca taataatatt
     9001 gcataaaatt atatctttat tttgaatatt atacgcaata gtaatttatt ttcattaatt
     9061 tattggtact aaattctatt acaatatatt aaattaataa atacttaaat gtatattaaa
     9121 attttaaaat tataaaactt attatcttat attgataaaa aataaaaaat tttaaaataa
     9181 tttgatttaa aaattgtatc caatttcata gattaatatc cgatccgacg aagaagtgag
     9241 aggtcgccaa ggtcctgaga tccgcgaaaa ttttctaagc tctcggaaga gatcagagat
     9301 gcggcgcgat gggtcggtat cttaggtcaa aatggtagat ggtgattgct gttactagct
     9361 ttgtcaacaa cttgaaataa ttggaggaag aagtgaggcg attaacatat aatttttgga
     9421 ggaataggat gaaagaaccc tcaattatgg tgatcctggg cctcgtcttc gccgccgcgt
     9481 ctccaggagg atcggcccag ctggacctct tctctggata ctggattaac gtcgaccccg
     9541 acgccatcgg agtcaccgcc ctagagatcg gagttactgg tccagaggtg aaagtcggcg
     9601 cctggggacg gtgccatccc gaggagtgct attggggcag cgcgaactcc tccatcgcct
     9661 acggtcctag gatcgattca gacctcgcct cggaggcgac ggccctatct gcaatctgga
     9721 gatctagctt cgtcgagacc ctgatggtca tcctcccctc agagccggat cagatccggg
     9781 ccgatgttta taccaggttc cctgatggga gcggtcggtc agcctacgcc gaaagtttcc
     9841 tcttcgagcg gagggaaggc gatagatgct ttcaggacct tccagacccc cagatcgagt
     9901 tgacgggaat agagagctat acggtgagcg gaaaaaactt cacccggtac aatctcgccg
     9961 tcgcgaactg gaacgcctat cctcctgaga tcttcgaggc ggcaccagac ctcccaccgt
    10021 gtggcctcaa cccccagtcc tccagaacct gggttgagat ccgcgaccag aacggcggtc
    10081 gaatatacgg cttctgcgcc ctcaaccacc ccatgaatct caccggcctc tggttctctg
    10141 tagaggaggg cgaagggcct ccggagtcgg cctacatggt gatgatggac cgacgttgcg
    10201 agcgctccct ccgctccaat gaggtctcca tcgacgacct ctcaaccccc actacgtcgg
    10261 tggagcctac cgacagcaag gccgccctcc ccgcaaacct tcaggccacg atgaactttc
    10321 agatgttctt tcccctctga tcaggggggg gcactcttca tcttttctcc ttctccaata
    10381 tcttcaaaat atcctcctcc ctcctcccct cttcagagat ccagccactt cccaaccacc
    10441 ccaattttga gccatttcga catattgata tcccatcggg acgaaaacat cttgaggtgt
    10501 tttcgatgaa ggcttcttcc tcggtgttgt tgatgggatt tttcgtcctc ctatcaggat
    10561 gcgtccagcc ccctgaaggg gggactatct cggagagagt ggcttctgag ggctggtcgg
    10621 cggacggtat cgtgggtgag aacgaatatt cg
//
LOCUS       DASM01000119            9309 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_268360, whole genome shotgun sequence.
ACCESSION   DASM01000119 DASM01000000
VERSION     DASM01000119.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9309
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_268360"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1716 a         2722 c         3249 g         1622 t
ORIGIN      
        1 ctggtcataa cctggcacga ggtctggggc gactactggc gggagtacct ggggaggatg
       61 gcgcccttcg ggattctggc agagcggggg gccctgaggc tccccgagag gatcatcccc
      121 gtctccgaaa gggtgagggc cgaccttttg gggatggggg tgggagccga tcggatggag
      181 gtggtcaata acggcgtaga cctggatcgg gtcggggcgg tgggggcggg ggaggccctc
      241 tacgacctca tctacgtcgg gaggctctcg gcccacaaga gggtcgacct cctcatcgag
      301 gctgtggggc tcctccggga ggaggtcccc gggatccggt gcgggatcct gggggacggc
      361 ccggagatgg cggcgctcag caggctcgtc caggataggg gtctggagga gagggtcgaa
      421 tttcttggct ttctggacgg ggaggatgat gtggtagcga agtttaaggc cgcgaggatc
      481 ttcgtcctcc cctcgacgag ggaggggttc gggatgagcg ttctcgaggc taacgcctgc
      541 ggccttccgg ccgtcgtcgt cgatgcaaag aggagcgccg ccgccgacct gatcaccgag
      601 gggaagaacg gggtcttgtg ccgaccttcg gcatcgtcca tcgcctcgaa ggtggcgggg
      661 cttctcgcgg gtggcagcta tgagaggatg gcgggaccgt cgaaggagtt tgcgaagggg
      721 tacgactggt ccctcatcgc tggaaagctc gaaagggtct atgaggggct ttgatgtcgg
      781 tggtgggatg aagatcctcc ataccccgcc ccgctacccc cccgccatcg gcggggtaga
      841 gaacgccgtc gccggcctcg caagagagct ttcggggctc ggccaccagg tctctgtcct
      901 ctgcgccgac gagcccgaat cgccgaaggt ggaggtcctc gagggggtcc gggtccggag
      961 gctctcctcc ttcgggaagg tcgcgaatac caacatcacc ccggggcttc ccatagcgat
     1021 cctccgggag gagttcgacc tccttcacac ccacctcccg accccctggt ccgccgacgt
     1081 cagcggcttt gcggccgcgg cgaagagaaa gcccctggtc ttgacatact ataacgatat
     1141 cgtcggcgag gggtcagcgg gacggatagc ccgcctctac aacggtacgg gtttgaggct
     1201 cctcctccgc ctggccgcga ggatcaccat catccagccg gagtacctca aatctcccca
     1261 cctccggggg tttgaggaga aggtcgaggt gatacccgtc ggggtcgacc ttgcgcggtt
     1321 caggccccag ggggcggctg ccgacgggag gaccctcttc tttttgagcc tcctggacag
     1381 gcatcaccgg tacaaggggc tggaggtcct cctctcggcc ctggcggtgg tgaaggagga
     1441 ggtcagcgac gtccagctcc tcgtcggggg cggcggggag ctcctaaatc attatcggag
     1501 ggcttcggcc cgcctggggc tggaggagaa cgtccatttc ctcggcttcg tccccgaaga
     1561 gatggtggcg aagcattata acagatgcga catcttcatc ctcccgtcca cctcccggtc
     1621 ccaagagggg ttcgggatgg tcgccctgga ggcgttggcc tgcggaaagc cggtgatctg
     1681 caccgggatc gtcggggtcg cggaggatgt cgagcgggtc ggggcagggc tcgtcgtcga
     1741 gccgaatgac cctgaagggc tggccggggc gatagcccgc ctcctccaga gcgggccgga
     1801 ggcccggagg atgggggcgg cggggaggag gcttgccgag gagaggtacg gctggaggaa
     1861 ggtggcggag aggttcgagg ggctgtacca ggatctggtc ggataggtgg caccggatcg
     1921 atagatataa aaaagtcatc gatgagaatc caggaggcgt caaatggacc tgaaagataa
     1981 gagagtcctc gtcaccggta tcagcggctt tgtcggctcg aagctcggaa agcggctggt
     2041 ggacgagggg gcggaggtct tcggactttt gaggaggagg gccgacggga tcctccccca
     2101 caacctgaag agggaggggg tgggacccca ggtgaggctt ctggaggggg acctggccga
     2161 gatctccagc ctgggggcgg cgctgacggc cagcgatccg gacctggtct tccaccttgc
     2221 ggcccagtcc ttcgtccccc gctcctttgc cagccccctg gagacggcgg aggcgaactg
     2281 cctggggacc gcaaacctcc tggaggcgat caggatcaag gaggtcgatc ccgtattcgt
     2341 cttcgccggg tcgagcgagg agtacggcct cgtcatctcc tccgaggagc agctccagag
     2401 ggcgcgggat aagtatggga cggtcttccc cgagccggca gaggtcccgg agctccccat
     2461 taaggagacg aacccgctgc ggcccatgtc cccctacgcc gcgagcaagg tctacgggga
     2521 ctttttgacg aggaactact gccactccta cggcctcaaa accgtagtat cgagggcctt
     2581 caaccacgag ggggcaggtc gaggcgggat cttcgtcacc tctgtcgtca cgagccaggt
     2641 gatgagcctc gtgatggggg aggcggatgg tatccggata ggaaacgtca acgccttccg
     2701 ggactggtcc cacgtcgacg acgtcatcga cggctacctc ctcctcgccg ataaggggcg
     2761 gttcggggac gtctacaatc aggggtcgat gaggacgaac tccgtcctca gctacctcct
     2821 cctcagcctg gagagggcgg gctaccccat cgacgggatc gagaccttcc ggggcgagaa
     2881 ggcggtggcc agccccacag agcaggaccc ctcggagatc ttcggatcga agtttgagaa
     2941 gacgaagatc gacgggatgc tcctccgggg ggaggtggag ttcggggccg aggacggcgg
     3001 ggtcgtcgcc ctctcagggg cggatagggt cccgatcgag ttcgatcccg agaggttcag
     3061 gcccgccgag gtcccgatcc tcttctcgga gaccgccaag atatccggcc tcgggttcgt
     3121 cgcgaggagg agcctcgggg atatcatcga ggaccagctg aaccactacc tctccgaggc
     3181 cgagaggaag gggaggggtt gaaggtgagg ggaaccttct gccggatcgg ggccgcccct
     3241 gggggcgagg cggtcgcctt cgcctcacac cggcggcagc ggatctctcc tgagaagaga
     3301 tcttacaggt ggtcccatgc cctttgagtt cgaagagctg ggccttccgg gagtacttct
     3361 gataaagccg aggatctttc gggacgaccg ggggtacttc cttgagacgt acaagaagga
     3421 ggatcttgcg agggccggga tcgagggcga gttcgtccag gacaactgct ccaggtccgc
     3481 gtacggggtc ctgaggggga tgcacttcca gcgagagccc tgcgcccagg caaagatcgt
     3541 ccagtgcgtc aggggcgtcg tctacgacgt cgccgtcgac cttcggcggg ggtccccgac
     3601 ctttgggggg tatgccgcct gcatcctctc cgagggcaac cgacggcagc tctacgtccc
     3661 ccgggggctc gcccacggtt ttctggtgct gagcgacgtc ggagaggtta tgtacaaggt
     3721 cgacagccca tactccccgg aatgcgaggg ggggctgatc tgggacgatc ccgacgtgga
     3781 cgtcccctgg ccggtcgacg atccgatcct cgccgagagg gatcggaggt ggcctgacct
     3841 gaagagcctt aaacgagatg gggatctgtt ctgaaagggg agatggtgat gcggagggta
     3901 atatgaaggc cctgatctta tccggagggc gggggacgag gctacggccg ataacccact
     3961 ctcaacagaa gcagcttata ccgatcgcta acaagcccgt cctcttctac gccatcgagg
     4021 acgtcatcga ggcgggggcc gaggaggtgg ggatcatcgt cggcccgaat aaggagcagg
     4081 tcatggagac ggtcaggtcg aaggcctggg gtgcggaggt ggagttcata ttccaggggg
     4141 agccgaaggg gctggcccat acgatcctcg tcgccgagga gttcctcgga gacgacgact
     4201 tcatcatgta cctcggcgac aacgtcataa agggcgggat cctcaaacat tctgagaagt
     4261 tccgggagtc gggccccgac tccctcgtcc tcctcacgga ggtggaggac ccccagaggt
     4321 tcggggtcgc cgacctggat gaggaggggc gagtgaggcg gctggtggag aagccgaagg
     4381 tgccgccgtc caactacgcc ctggtcggga tatacttctt caggccctcg atcgtcgagg
     4441 cgtgcaagag gataaagccc tcgtggcgga acgagctcga gatcaccgac gccatccagt
     4501 ggctcctgga cagcggccgg cgggtggagg cgtccatcgt cgacggctgg tggaaggaca
     4561 cggggaaggc cgaggacatc ctggaggcga accggctgat cctcgacgag atgaggccga
     4621 agaacgaggg ggttctgaga tcctccagga tattcgggag ggcggtcgtc ggcaaagggt
     4681 gcgtcatcga gaactccgag atcaaggggc cctgcatcct cggggcaaac tgcgcgatcc
     4741 gggactccta catcggccct tacacctcca taaacgacgg ctgcaccatc gaggggacgg
     4801 aggtggagga cagcgtcatc atggaggggt gcaccatatc gagggccgga aggatcgtgg
     4861 agagcctgat aggaaagaac gtcgtcatcc gggagaacgg ccgaaggcct gcgggccaca
     4921 ggtttatagc cggggacagc tcggagatcc tgttgtgatg ctatgaggct tctcgtaaca
     4981 ggcgggatgg ggttcatcgg gagcaacttc atccgccact atctggagag gcaccaagag
     5041 gcccggatcg tcaacgtgga cgccctgaag gcgggctcca accccgagag cctgaggggg
     5101 atcgacgagg agaggtacac cttcgtcgag gcggatatct ccgaccgccg tctgatggcg
     5161 gatcttgtag agaaagccga tgcggtggtg aacttcgccg ccgagaccca cgtcgaccgg
     5221 agcatctccg gccccgagcc gttcctttca agcaacgtcg gtgggacctt ctcgatcctc
     5281 gaggccctga ggacgagaaa ccccggggcg aggctcgtcc acatatcgac ggacgaggtc
     5341 tacggagacg tcctctcggg ctcctttcgg gagaccgacg ccttaaggcc gtcctccccc
     5401 tactctgcga gcaaggccgc ctccgacgtc ttcgtctccg cctacgccag gacctacggt
     5461 ttatccgcca tgatcacccg gtgcaccaac aactacggcc cccgccagtt ccccgagaag
     5521 ctgatcccca aggcgatcat ccgggccaac acctccctga aggtccccat ctacgggacg
     5581 ggggagaacg tccgggactg gattttcgtc ctcgaccact gccgggcggt ggagatggtc
     5641 ctggaggcgg ggggggccgg ggaggtctat aacgtctcct ccggggagga gaggacgaac
     5701 ctcgcggtgg tgagggccct cctcgagatc ctcgggaagg gcgaggaggc gatcgagttc
     5761 gtcgaggacc gccccggcca tgacgtcagg tacagcctcg actcctcgaa gatccgaagg
     5821 gagctaggct ggcggccgga ctggggcttc cgcgaaggca tggaggagac ggtccggtgg
     5881 tacgtcgaga acgaaagctg gtggcggccc ctcatcgacg atcgggtcct ccacccgacg
     5941 ccgtggaagc tggagtggtg aggatgcggc ttctcataac cggggggagc ggcctcttgg
     6001 gctccagcct cgccgaggtc gccgtcgcgg aggggcacca ggtcttctcc ggatactgcc
     6061 gccattcccc agagtttggg gtggcggtga agctcgacct gctggagggg gcggaggtcg
     6121 agaggtcgat ctcggaggtt gcgccccagg tcatattcca caccgccgcc ctgaccgacg
     6181 tcgacaggtg cgaggatgat ccggatctag cctggaggac gaacgtcctg gggacggagc
     6241 gggtcgcgag ggcggcggca gaggtggggg cccatctcgt ctacgtctcc accgattacg
     6301 tcttcgatgg cgaggggggg atgtaccggg aggaggatcg gaccgacccc gtcaactgct
     6361 acggctggac gaagctttcg gcggaagggt tctgcgacct tgtcgccagg acctctgtca
     6421 tctacggggc gaggcccgcc agcggcaagg tcaacttcgc cctctggctc ctcgagaggc
     6481 tcgccgccgg cgaggaggtc cggatcgtca ccgaccagtt cataaccccc accctcaaca
     6541 ccaacctcgc aaggatgctc ctcgaggccg gggagaggag gctatcgggg gtctatcaca
     6601 tggcgggggc gacgagggta tcaagatacg acttcgccgt cgagatcgcg agggccttcg
     6661 gcctcgacga ggggctgatc gccccctcca ggatctcgga gatgaggtgg agggcgaaga
     6721 ggccgaggga ctcctccctg gacacctcca aggcggcccg gctcctggcg gagggcccct
     6781 cgcccctccg ggagtcgctg agggccctga gggaggagat cggagagggc gatctaaaat
     6841 tataagctaa ataaataatt attggaggat acatattggt caccttggtt acgggcggtg
     6901 ccgggttcat aggctcgaac ctggtggagg cccttctggc tagccgccag gaggtggtcg
     6961 tcctggacga tctgagcacg gggagcaggg agaacctgga ggggctgtcg ggaaggatcg
     7021 acctcgtcca gggaaggtcc tcagagctcc tcgatctgaa gatcaggcct gaggccatat
     7081 atcaccttgg aataccgtcc tcctcgccga tgtacaagag ggacccgatg ctggtgggcg
     7141 aggcgatgaa cgggatgatc gccgtcctgg agctggcaaa aaagaccggc gcgagggtcg
     7201 tctacgcctc cacctcctcc ctctacagcg gccagacccc gccccaccgg gaggagatgg
     7261 aggtcttcgt caccgactac tacaccgagg cccggtttgc catggagagg atggcggaat
     7321 tgtacgggaa gctcttcggc gtcagggccg taggcatgag gttcttctcc gtctacggtc
     7381 cccatgaggc ggccaaaaag aagtatgcga acatcgtcac ccagttcctc tgggagatga
     7441 tggcggggag ggctccggtc atctacgggg acggcaccca gacccgggac ttcgtctacg
     7501 tcagggacgt cgtctccgcc ctccagctgg ccatggcctc cgattatgcc ggggcgctga
     7561 acgtcggcac cgggagaagc tacagcttca acgccgtcgt cgagatcctg gcggagagga
     7621 tgggcctgga cctgaaggcc gagcacgtcg ataaccccat caaaaactac gtccgggaca
     7681 ccctggccga taccacaaag gccgaggagg tcctgggatt cagggcccgg tactctctgg
     7741 atgaggggat cggggagctt ttgaggtgct acggattcag aggatgatcc ccccgagggg
     7801 gcggcggatg aaggggatgg ctgaggaggg tccggctcgg ccggggctga taagcgtcgt
     7861 catcccgagc tacaacggga ggcggtacct ggaggggtgc ctctcctccc tctcggtcca
     7921 gagcttcgat gacttcgaga cgatcgtcgt cgacaacggc tcggaggacg ggaccgccga
     7981 atacgtggcg gcggagttcc ccgaggtgag ggtggtgagg agcgaggaga acctggggtt
     8041 cgccgccggg gtcaacgccg ggataaggcg ggctcggggc gaggagatcc tcaccctcaa
     8101 caacgacacc gtcgccgatg aagacttcat caagagcctc gccaaggcga tggccagaga
     8161 cgatcgggtg gggatgtgcg cctccaagat gctctttttg gacggaagga tcaactcggc
     8221 ggggatctgc ctctcccgga gcggcgccgc ctgggaccgg gggatgggcg aggaggaccg
     8281 gggccagtac gacgccctgg aggaggtctt cggcccctcg gcgggggcgg ccctctaccg
     8341 gcgaaagatg ctcgaggaga taggcctctt cgacgaggat ttcttcatgt acatggagga
     8401 cgtggacctg gccctccggg gccggctcgc cgggtggagg tgcctctacg tccccggggc
     8461 ggtcgtccgc cacgtccacg gggggaccgc aggctttggt tcagacctct cggtctacta
     8521 cgggaacaga aacgtcctct ggtaccccgt caaggacctg ccggcgaggc tattgatccc
     8581 gtccctgccg tggatcgtcg gccggtccct cgccgtcatc ccctactacg ccctccgggg
     8641 gcggggctgg accgccctcc gggcgaaggt cgacggcctc gctggggtgc cccggatgct
     8701 gaggaagcga aaaacggtcg tgcggcgtct cccggcgggg gagatacgca aacatataag
     8761 gacgtggagc gagatcaggg agccctgaga tgtggatcgt cgagtacagg gaattgatac
     8821 ggatcttgac cgtcagcgac ctgaaggtga agtaccagag ctcggtcctc ggttttctct
     8881 ggtcgctatt gaacccctta ctgatgatgc tcgtccttta cttcgtcttc agcaacgtct
     8941 tcaagttcga ggacgacagc ttcgccctgt acctattgat cggcatcgtc gcctggcggt
     9001 ttctcgccaa cggcaccatg acctccatct cggcgatagt gggaaggccg agcctcgtca
     9061 ccaaggtcta cgtccctcgg caggtcctgg tcctcagcac cgtcctatcg agcttcgtga
     9121 gctcgatcct cgagttctcg gttctgatac ccctcctcat cttcttcggc gtagacctat
     9181 cgatcaacgt cctcttcttc cccctgatcc acctcgcctt cctcggcctc gtctacggga
     9241 tgagcctggt cttatccgcc ttatacgtct actaccggga cctgaaccag atctgggacg
     9301 tcctcctcc
//
LOCUS       DASM01000120            5570 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_231857, whole genome shotgun sequence.
ACCESSION   DASM01000120 DASM01000000
VERSION     DASM01000120.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_231857"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          909 a         1970 c         1711 g          980 t
ORIGIN      
        1 gaacctccat cttgaaggcc gtcccgggga cggccttcaa gatggaggtt cgggagtgga
       61 gggtcagcga acgccagggg ctcgttgaga tcccctcctt cggggaggtg gcggcggata
      121 agaggtggta tgccgaggcc ttccgcctcc actacggcga gcaggaccct ttccggggca
      181 gaacgatagt ccagccccac cccaagaccg tcgtcgtcgc aaacccgccg tccctccccc
      241 tgacgacgga ggagctggac gagatctacg agctcccctt cacccgggcc gcccatccct
      301 cctatcggga ggagataccc gccctggagc cggtccggtt ctcgatcacc acccatcggg
      361 ggtgctttgg ctcctgctcc ttctgcgccc tcacccacca ccaggggagg atcgtccaga
      421 gcaggtcgat cgagtcggtg gtgagggagg cggaggctct gacgagcctc cccgggttca
      481 agggggtgat ccaggacgtc ggcggcccca ccgccaacat gtacgggatg gcctgcagcc
      541 gctggcggga tggcgccgga ggcgtcggcg cctgtgccga caggctctgc tcctctgact
      601 gtcctactct gaaggcagac caccgccgcc aggtggagct cctccgccga ctgcgagaaa
      661 ttccgggcgt caagcgggtc ttcgtcacct ctgggatccg ccacgacctg atcctggcgg
      721 accggaccgg cgcgggggag gagtatctct acgacctctg ccgccaccac gtctccggcc
      781 atctgaaggt ggcgcccgag cacgtctccg atcgggtcct ggcgaggatg cataagcctc
      841 cccgggccgc cctggaggag tttcgggacg agttcaaaag ggcgagccgg aaggtgggca
      901 aagagcagta cctcgtcccc tacttcatct ccggccatcc cggctgcgag ctccgcgaca
      961 tggtggagct cgccgagtac ctccgggacc accggctccg ctccgagcag gtccaggact
     1021 tcacgccgac gccgatgacc gtctcgacct gcatctacca caccggcctc gaccccttca
     1081 ccggcagtcc cgtccacgtc ccccgggggc gggagaagga gatccagcgg gctctcctcc
     1141 agtactggga taagaggaat gaggggctcg tccgggaggg cttgcgcctt gccggccggg
     1201 aggatctcat cggcgacggg ccggaatgcc tggtgccgaa ggggtgatcc ggcttcctca
     1261 ggtcccctcc cttccggagg gtttgaggac ccctccagga gatgggacgg ttgggccgcc
     1321 agctataaat atgtataacg agtaaatata cagtttgccg cctcgtgcaa gggttggtgg
     1381 gcggaccgtg ggctgaacgt tcccgacgat gctccaatat ccggggtgac tgaagcctgc
     1441 tcctcaaccg gaggagcatg gcgtggcctg gaaggctggc cgatcaggac cctccgggat
     1501 aggagatgca cgccttcgtg gcaacgcatt cgggcaacga gccagcgaga tgagagctcc
     1561 ggcggcgcgg aaaagacttg cgcgccgtga tgggcgcagc aattcgccgg ggaactcgcg
     1621 ataatacgcg gtcgggagca tggggcggca cattccctcc tcttccgata gttaaatata
     1681 tttttctgga acaaaaaatc tcttctgccc tcatcggccg ccattaccgt cccagaggaa
     1741 gagaagctca tgggggcggc cgccggaaag tttgagcccc acctctccat agctggtgcc
     1801 gagccgacca ataatagtgg atagaagtcc aaatacagct ctctaatatc cgcgatcttg
     1861 aaaggaggga ggagcaaaag tcgttatagg agggtagagg gagagaatta gcctacgact
     1921 tttgccaatt actttttacg gcaattaacg aatatatact tttcggttcc tctagggtcc
     1981 cggccccggc cttccgcccc tttggccgcc actctcttat gaacggcgaa gtcgatgacg
     2041 atgttgtact ggtggggggc gtagctccgg accacccctc ggcggagaat ctcggccttg
     2101 acgccggagg cagaagccgc ctcttcgacg aaagccgtat ccctgccgaa gaggtcgtcc
     2161 tcgggggcga tggcgtagta gtggacgacc ccgccctcct tcgcgatttt catcgccgcc
     2221 cccaggaagt cgcgagcgga gtggggcagg ttcatgatga cccggtcggc ccggttcgcg
     2281 aactcctccg cgagggccgc ggcgtccgcc tccataatta ccatatcggt gatcctgttg
     2341 aggttggcgt tctcccggag gagcctcaca gcgtcggggt tcttgtcgac ggcgacgacc
     2401 ctcgcccccc gcttcgcgat gaggagggcg aaggggccga ccccggcgaa catgtcgacg
     2461 gccgcctccc cccgcctcac caggcttgcg actctcagcc gctcggtgcc gaggcgcggg
     2521 gtgaagtagg cccgctccag gtcgattctg tacctcaaac catgctccct gtggacggtc
     2581 gtagtccgcg cctccccggc cacgtgttcg aaacgccggg tccggtactc cccctccacc
     2641 gccgtcaggg gcgatatcac cgccctgatg ttcctgtgga cggcgatgat ggcggcggcc
     2701 gcctcctgat ccgccccctc caggatggct atatccccca ccacctcgaa ggaggctttt
     2761 cggccgagga gcccctccac cgtctccctc gtctcctcct cacgaaactc cgcctcgacg
     2821 agatcgaact ctccgaggct ggagagctcc tccttcgcct cgcgggagag ctccaggatc
     2881 ggcatataaa ggtcttcgcc ctcggagcgg atcttccgcc ccggatcgaa ggcccccatc
     2941 tcgacgagcc tccgccgaaa gacctccccc tccctcctcc ggaccttcag ccccagggtc
     3001 gacgccatct caataccccc ttccgctccc tcaccggccc tcggctccga cgaggcccgc
     3061 cgcccctcgc tgctcctcct tgatggcggc gtagatctcc tcctggcttg gcctttcaat
     3121 cctgacgtac ctcccaccgg cggcggcgct gatccttccc gcgagctcgg agcccccggc
     3181 gccgacgtcc accaccagga gatggaaccc agacctcttc agctcccggg ccgcctcctc
     3241 cgcctcctgg ccggggtccc ccgccacctc cagggggacg ttggcggagc cgtcggtgat
     3301 caggacgagg atcgggatcg ccgagggctc tttatcccgc tcccgggcga ggagggcgga
     3361 gcctttggcg aggccgcttg cgaggggcgt cgtaccgccg tactcgatcc cgtcgaggtg
     3421 cctcttcgcc gtcgccgccg ccggggtgaa ggggaggacc accgccgcct ccctccccga
     3481 gcaggagacg agggctaccc tgtccctcct ctggtaggcg tctctcagaa gctccaagac
     3541 caccctcctc gtcgcctcgg aggcctcctc catcgagaag ctggtgtcga ggacgatagc
     3601 gacgtgggtc gagaccttcc gacgcctcac cttctcccgg aggtcctcct ccctgacgga
     3661 gaagccctcg actccggcct cacccccggc cctgacggag ccgccggatc gggccagggc
     3721 tgcccggatg gtgggggcga gggcgacgtc ctttgtcttg gagcggggca tccgggcccg
     3781 gacgtaccta ccccgctttc cccgggccaa gacctccgcc ctcctccccg aggccggcga
     3841 ctttgcggcc gccttcgacc ccgaccgggc gaagccgtcg aggatctcgc cgaggtcgca
     3901 ggcctcctcc ccggagcggg gccgccggag gagcgcttcc agctccggga cccccatctc
     3961 gatgaaggta tcgtcgccga ggaggacgac gtcggcgatc tcttctagcc tcacagaagg
     4021 gcctcggctc tccaggacgg acctgatccg cccggcatcc tcgtcggtca tcatccccgc
     4081 cctcttcctg gggagcctga tccccaggac cggggccccg gcgaagaaga ccttgcaccc
     4141 ccaatcccga tcgaggttcc tccggaggag ggggatcagc tctcctgcct ttgaggatgg
     4201 ggattctgac ggctccatca gcccaccagc gatcggagcc tctcggggct gaaatcgcct
     4261 tcgtcgaagg gggtcttcct catcctgtga ggcagcacca tctccgccgc ggcgacgatg
     4321 tcggcggcct cgacccgctc cctcccctcg aaggcggcgt tagccctcgc cgtccgctcg
     4381 atcatgatgt cggcccgatg gccgtcgacc ccaaactcga tggctattct ggctatattt
     4441 cggagctcgc ccctcccgac ggcgaccctg gggagggcct tcttggcggc gacgatccgg
     4501 gatgtgagtt tcctattctc ggcctcaaac ccctccctga acttggcggg atcctcggcg
     4561 aaggcgttgc tcctctcgat gatctccacc cgctgctcga cgtcggatat cccctccacc
     4621 ttcacctgga gggcgatcct gtccagaagc tgggggcgga gctccccctc ctccgggttc
     4681 atcgacccga cgatgatgaa gctcgcgggg tggctggcgc tgaccccctc ccgctcgacg
     4741 acgttcatcc ccatggcggc ggcgtcgagg agggcgtcga cgacgtagtc gtcgaggagg
     4801 ttgatctcgt cgacgtagag gatgccacgg ttcgcctccg ccaggacccc gggctcaaag
     4861 gccctcaccc ccccccggag ggcccgctcc atgtccaggg accccaccac ccggtcctcc
     4921 gtcgccccga cggggaggtc gacgacccgc atctgcctca actccgtcgg caaaaccccc
     4981 tccgccagcc gctcggcgca ctcccagcag agcttctccc catcccgggg atcgcagccg
     5041 aaccggcagc ccaccaccac ctcgatctcc ggcaggacct cggcgagacc ccggacggcc
     5101 gtcgacttcg ccgtcccctt ctctccccga atcaggaccc ccccaatcga ggggttgatg
     5161 gcgtttagga tcagggcccg cttcatcctc tcctggccga cgatggcggc gaatgggtag
     5221 aggagcctct tcgacctcgc caggtggagg gcgaaggatc cggacgtccc gctttctgcg
     5281 gccgttgatt cgggccagcc cgatctgtcc ggggccttcg aagcggccgg atcccgcccg
     5341 cctctgctcc ttctctcgtt cttatccgaa gatcctctca tcgtggggtc cgagatcgag
     5401 ttcgcagccc cgccggagcc ggccttggag gccgacccgg gggaagagcc cctttccctt
     5461 atgacgccag tgatcctcaa tatcttcacc tcatgagtac ctgagcatga tcgccgagag
     5521 gagggtcacg atgagcagga ggagccccag aaagtagagg ccgaagaggg
//
LOCUS       DASM01000121            7121 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_131903, whole genome shotgun sequence.
ACCESSION   DASM01000121 DASM01000000
VERSION     DASM01000121.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_131903"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    317..332
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1175 a         2336 c         2147 g         1447 t
ORIGIN      
        1 agttcatcca gctctggggc aacggaacct gcggcgaggt ccaggtggac tggctgccct
       61 gcatctaaat tcgaaattat taggctgccc ctaaagccaa atcggggcag tccccacctc
      121 tttatcgatc tttaatgatg gacgatcggc atagatcgtc tcgccttagc gatttttctt
      181 cgccccatca aggccgcggc gagccccaac ttcaatcgtc gaatccatat tcttggcaaa
      241 atgaaacatc gtggaaatta tatggcccct aaatatccga ttttttaaaa ggatgctccg
      301 gtggggattt gaacccnnnn nnnnnnnnnn nnttctacca tttaaccctt ttgccccacc
      361 cggccggcgc tggggcagac ctcggcgagg atacaggccc cgcaggaggg ggacctcgcc
      421 cggcagacgg cccggccgtg cctgatcagg tttacgtgga aggagcagta cttctcccgg
      481 ggggtgatct cctccaggat ccgctccgcc tcctctatgc tggcgccggc ggggacgaag
      541 ccgagcctcc gggagaggcg gttgacgtgg gtgtccaccg ggaggagggg catcctgaag
      601 gcgaagagga ggacgacggc tgctgtcttc ggccccaccc ccgggaggct caccaggaat
      661 tcgcgggcct cctccggatc agcgcccctc agaaactcca ggtctatccg ctccggccct
      721 cctgcctcct ccctctcccc catttcatcc tcttttcccc ctccatctcc tcccctcggc
      781 cctcccctca ctcctcctct cgcgatcttc ctctcgcgct gcctctcctg gatcatcgcc
      841 agggcctcct tgatcctccg ggtcttgatg ttggcgaggc cgccacagcg gatcagcccc
      901 gccacctcct catcgggggc ggagaggagc gcctggtatg cggggtaggc cctcttgagg
      961 ctctcgaagg cccggtgggt gttagccgag gtggtgttct gggagaggat cgtctggacg
     1021 aggaggtcga cagggtcgtc cccccgcctc ctggggaggc cgtaggcctc ctccaacagc
     1081 tctatgcacc ggtcgagccc cctcttcgtc tccgcgggga ttggatcctt ggaaggaact
     1141 ttggctccgg gattggtcgc ttccatccct cctccgatcg ccccggaggg tcaataacct
     1201 gacgctttcc cctccgggag gggcgcccct cgctggcttc tccccccatc cccatgccgt
     1261 ataaagaccc gctctcttcc tatcctcacc atctacccat cttcccatca tcaccatcta
     1321 cccatcttcc catcatcacc atctacccat cttcccatca tcagcatcca cccatcttcc
     1381 cctcctcatc catcctcctc cccatctttg ccggcggctc cacggaaaaa ttattagccg
     1441 aaggcggcca gggtttgtgg gatggactac ggcagcctga gcctggaggc gatggccttc
     1501 agggacctct accccttcct catctcgatc ctcatcgggg ccctcatagg gacggagagg
     1561 gagcgggacc tcctggagaa gaagaagcgg ggggtggcgg gcctcaggac cttcatcctc
     1621 ataaccctcc tcgggaccct ctcggcagcc atggcggcag agtacggcgg cgccttcatc
     1681 atcgtcgcct tcgccagctt cgccctgatg gtggcggtgg ggtacgccgc gagcgcgacg
     1741 aacctgggga ggctcgactt cacctcggag gtggcggcgg ccatagcctt catcctcggg
     1801 gccctctgct accaccccga tacggtgatg ctggccatcg ccctggccat cctggggacg
     1861 gtgatcctgg cgatcaagaa gaccgcccac cgctacgtcg agatcatccg ggaggaggag
     1921 ctcctcgaca ccctcaagat ggggcttgtg gccctggtga tcctccccct cctccccaac
     1981 accaccatcg accccttcgg ggtcctgaac ccgcggaaca tctggatgat ggtcgtcctg
     2041 gtcagcctga tcgggtacgt cggctacgtc atgatcagga tcttcggaac ggataggggc
     2101 ctatccatca ccggggccct gggggggctc gtctccagca ccgccgtcgc caccaccatg
     2161 gcggcggagg cgaaggccca ccgggaggtg gtcccctcgg cggtctttgc caccaccatc
     2221 gcgagctgca ccatgttccc ccggatcctc ctggaggtct tcgtcgtcaa cagggccctc
     2281 ttctttcccc tcctcatccc catggcgagc atgaccgtcg cgggggtcgc cctggccctc
     2341 ctcctctaca ggaagaggga gacgatcgag gcgcccgtaa acctgaccga tcccttcagg
     2401 ctctcgcctg ccctcaagtt cggggccttc ttcgccctcg tcctcctggt ctccaaggtc
     2461 gcgagcatct acttcggcga ggccgggacc tacgctgcga gcctggtggc ggggcttgcg
     2521 gacgtcgacg ccataaccct atccctggcg accctcgcca ggaccccggc catgggggat
     2581 gaggtggcgg tggcggcgat aaccctcgcc gccatgacca acaccctggt gaagctcgcc
     2641 atcaccttcg tcctgggcac aagggagttc ggctcccagg tcgctttgat cttcatcccc
     2701 atgatcgggg tgggggtatt gactataatc ctcttctagt tgaaagaaaa ataggaggcc
     2761 gcatccctcc acctggtgcg gcaggagtat cgatggcgat cagctcatcc tccgatgaag
     2821 accttccgga ccacctccga gaggattggc cctcccctgt cgacgacctt cgcctcgtag
     2881 atccagagct cctcccttcc tagggtcccc tcgacgatca tcgccatccc ttcgtccttt
     2941 gaaaggtcga tctggtgctt ttcgatcgcc gcctcgtcca gtagcttcgg ctttatcagc
     3001 ttggttagcc tcacggcgca gcagtgctcc ttcggctcga ggatcctgaa ctgggcgctc
     3061 tcgaccaccc ccaaaaactt cgtcatcaat ttcacctccg ttatcttcat aaggttgcga
     3121 tcgaatttat attttttggc ttcgattatg tttcccgaag ggtgatggcg gattggagag
     3181 ttggagaggg atatggcggc ctgaggacga aaaagaactc acctccctta caccccaaag
     3241 ctctggaggt atcccctgag ggtgcagggt ccgtcggcgg ggccgatcgt ctcgaaccgg
     3301 tcgcagggcg tcccccgata gcatggaagg tcgtggatgg cgcagagggc gacctccccg
     3361 gtaaaggcga gatgggtaca cctctcgccg gcgggctttc gtatcatcgc ctccgggtcg
     3421 ccggggtcga gggttccgtc ggggcgtatc gaccggggat ttattataaa tatatccaga
     3481 tttatgcagc agaggccgca ccgtgagcag atcatgatca ggccttcctt ctccgagccg
     3541 atattccgtc aaacccatcc cgggaggaga tgatggccca ggagacctca tctcgccgag
     3601 gaggcccccg gtttggcggg gcccgccagg ccgccccagc tccccatcga gcattctgga
     3661 acggtcaggc gagcttgctc ttgaccttct cgtagaaccc cccctctcgg gccttgacga
     3721 atcgggcggg cttctcggcc cggctgatgg tgatgtggtc ccttttggag atggtggcga
     3781 cgttgtggcc gtcgatgacg accagggcct ccttctcggg gagctcgagg tcgaccttta
     3841 tcaccgactc ggcggggagg acccaggggc gggccgagag cttgaagggg gcgaggggga
     3901 cgaggacgac ggcgtcgacc ctggggtcga cgatgggtcc tccggcggac atggcgtagg
     3961 cggtggagcc ggtggcggtg gcgaagagaa ggccgtcggc ccggaggctc tccaggaggg
     4021 aggcgtcgac ggagatctca aactccagca tcttggcggg gctcgccgag aggagggcga
     4081 tctcgtttgt agccgggggg agggggacgc cgttgaggcg ggtcgaaagg cggctcctct
     4141 ccatcacctc gaagccgaag aggacccgct ccagggtcct gaccgcgtcc ttcggctcga
     4201 cgtcgacgag gaagccgagg gtccccatgt tgatccccag gatcggcctt gggtcctcca
     4261 tcctccgtat cgtcctgagg atcgtcccat ctcctccgac ggagacgatg aagtcgacgt
     4321 ccatatcctc gatccgggtc tcggcgaagc cgagcttctt cgccgtctcc ggctcgatgg
     4381 agatcccggc cctggaccgg aacctctcga ccaggttacg gaccaggttg atcgactcgg
     4441 ggtcgccccg ggagacgaag cctattctaa cctctgccac cccccagaag gtccaaaata
     4501 tcccggtgga agatgccgtt tgaggcgatg tactcagact tctgccacat gtcgccgttg
     4561 aagccgatct ggtcaccccg gccgtcggtg acggtcccgc ccgcctcctg gacgatgagg
     4621 ctcccggcgg cgacgtcgac gaggcggagg ccgccccgga ggtcgacgaa ggcgtcgagc
     4681 ctcccggcgg cgacgaggca gagctccgcg gaggtggacc ccagggtccg gatccgccgg
     4741 acgacgtcgc cgagggggac gatccgttcg gtgttggggc ggagggtgta ggcggagacg
     4801 ctgaagtccc ggagggtcgg ggtcttagag acgccgaggc gggcccgcct cccggcggcc
     4861 tcagaccagg ccccccgccc cctctcggca aagtactgga cggcatgggc gaggtcggag
     4921 acgtagccga agctggaggt ggggccgctg atgtagatcg agaccgagta gaaggggatt
     4981 ccggcgatgg cgttgaaggt cccgtcgagg gggtcgaggt ggaggaagta gctcgggcat
     5041 ttgccgatct cctttctccc tccctcctcg gagaggatgg tgaggtcgat ccccgacccc
     5101 tccaggacgg cgaagaccgc gtcctcggcc cgctggtcga tgctcttggt gggggtgccg
     5161 tcggccccca tgtggatcac ccggccgctg gcggggtctc cgaccatctc ttcgatcgcc
     5221 agaaagacgg cttctgcagc cgagttgcag atcccaagaa gcctcctccc ttcgggggag
     5281 aggcccatct ccacctcttc ttccatcagg gagtaccagg acgagatctc ttataaaagt
     5341 ccccccccga ccgacggttg ggaattattt tagacgttaa atttaaacgt gatatattgc
     5401 tacacgctat cacatatcag atgaagggcg ccgctccgcc gggccaatcc ggcggttttg
     5461 cgccacatat gtacaaaaat gatcaacgat gcgaatcatt tcgcggaggt agtttgattg
     5521 tacggattgc cctttgagac tctatccttg gtcatcgccg cgccggtgct gatctccctg
     5581 gtgctcctat tctgggcgct gaggtggtga aggagttgaa tttgaacttc gagatgttgg
     5641 cgatcgtcgc cgtctacttc ctggggctga tggccgtggg ggtctgggcc tccaagaagg
     5701 tgagatcttc ggaggactac atcctggcag gccgaaacct cggcttctgg gtcttcacca
     5761 tcctcatcgt cgccagcatc tgcagcggga tgacgatcct ggggacgagc ggcctggggt
     5821 atgtgacggg atggcctacg atatgggagc aggtcttcgt ccccctcagc gctgcggtct
     5881 gcatcctcct cttcggaact aagctccacg ccatcgggcg ggagcggggt tacctgacgg
     5941 tccaggacta cttctcggac cggttctaca gcaagaacgg gatccggggg ctatcggcgg
     6001 tgacggggat tctcgtctcc ctgatctaca tggtgggcca gttcgtagcc atcagcatgg
     6061 tcctcagctg gctctttggg atatcctacc acaccgccct gatgatctcg gccctgatcg
     6121 tcaccgccta cgtcatcatg ggggggctct acgccgtcgc ctggtcgagc ctgatccagg
     6181 ggctgatgct gatcgtcggc gttctgctgg tagccccggc ggtgatccga tcggcggggg
     6241 ggctcaccca catcaacgcc gtcctggcgg ggatcgaccc gaacttcgtc cagccctggt
     6301 acccgagctg gtaccctgag atggcggggc cctacgccgg ttacgccttc gccacgccca
     6361 tcttcctcct ctccttcttc ttcctcctcg ccttcggcct cgccagcgcc ccccacgtca
     6421 taaacaacgt cttcgccgcc cgggacgcca ggtacttccg ctgggcgccc ctcgccgcct
     6481 tcgccgtcta cgtcgtcatc atgtacctga tcaagatcac ggggtatgcg gggaggtcga
     6541 tggccgccga ggggatgatc agccttccta caaacgtctc aaacccctcc gattacgtct
     6601 tcatcctggg ggcggagtac gccttcccca gcatcatcag cgtcttcatc ggggtgatcg
     6661 tcctcgccgc cgtcatgtcg acgacggacc ggctgatgct gaccataggg acctacttcg
     6721 gatgggatat ctacaagagg ttcctccgcc ccgacgcccg ggatccgacg atcaccctcg
     6781 tcagccgggt ggcggtggcg gtctcggcgg tcctcaccct cctcctggcc tggagcaagc
     6841 ccccggagct tctggcgtgg ctgatatgga tggggatcgg cctcatgctc gcctgctacg
     6901 tggcgccgct cctggccggc ctttactgga ggcgggcgac gagggagggg gcgatcttct
     6961 ccatgggcct cggcctcctc gccgccgggt ttgcgggtta ctggcaccag ttcgttcagc
     7021 ccctcccgta ccacttcagc ctcttgggct tcgtcatatc gatcgtcgcg atcgtcgtcg
     7081 tcagcctcgc cacctcgaag ccctccgaga aggtcctcga c
//
LOCUS       DASM01000122            9244 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_39135, whole genome shotgun sequence.
ACCESSION   DASM01000122 DASM01000000
VERSION     DASM01000122.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_39135"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2011 a         2757 c         2675 g         1799 t
ORIGIN      
        1 ccccccgaga acgtcgacgt ctacatggtg gcgccaaagg ggcccggcga cctggtccgg
       61 aggacctacg tcgaggggat aggaacgccg tgcctcgtcg ccatccagca ggacttcacc
      121 ggcaccgcct tcgataaggc gatggcctac gccaagggga tcggcgggac ccgggccggg
      181 gtcctggaga cgaccttcca ggaggagacg gagaccgacc tcttcggaga gcaggtcgac
      241 ctctgcggcg gcgttacaga gcttataaag gcctcctttg agaccctcat ccgggccggc
      301 taccagccgg agatcgccta cttcgagacc tgccacgagc tgaagctnnc gtcgacctga
      361 tctacgaggg cggcctgatg cggatgtggg ataacgtctc caacaccgcc gagtacggcg
      421 gcctcacccg gggcgaccgg atcatcaacg agcagtcgag ggaggcgatg tgggagatcc
      481 tgacggagat ccagaccggg gagttcgccc gggagtggat cctggagtcc caggccggcc
      541 ttcccgtcaa gagggccctg gagaggatgg agtccgagca cctcatagaa gaggtggggg
      601 cggagctccg ggccatgatg ccgtggctga agaaagagtg atgatcatct cttaaaggag
      661 ggatgctaag ccatcgggag ggcgggcgcc aaggcgcgtc cgcccgtccg gagggctcat
      721 atttatccct tgattttctg gaagtatctc actgagattg actgcgtgct gtacaagcag
      781 ccaatacccc agctccaaaa gcacaacact tcggcgattc cataatttag ccatggatct
      841 atatggcatg caaactgtac catcggcgat tgaagcgatc tttgatcaag gtgggtgccc
      901 aattctctgc cctaaggcgc atccgtgacc agctaagaag ttagggttcg cggtgcaggt
      961 gactcccacc caaatctggt cgacggtttc tggccgggcc gcgcacgcat tccatgccat
     1021 cgtatagaga tcatctgcga cctcctgctg cctgccagaa gccactatat catcatctag
     1081 atattggatg acaagctcga ctccaggctc gggtacccaa ggtcgatcgt atatcttaac
     1141 ttcctgaggg taatatcccg ccaaggccgc attggtaaga atctcatcaa cgatatcttc
     1201 tctcacgcca agtgtcaggg ggatggagct atcgtcgctg gccgccacta tcgttgccat
     1261 catcaatcct atggtaaccc aaccgagtag tttagttttc atatctacca caatccctca
     1321 ctctgagctc tctcgggaat aatatcaagc cttttgctga tcatccttta tctttttgac
     1381 atttctttca attccctttt catcgagacg ctcgttgcag ataatcatta taaaaccata
     1441 atataaataa gtttcccact taaatcatca ttttttttat caggatgacc cttgtctccg
     1501 caaggaagtt ttccttccca acctccacaa gtaattcccc gccgcccttt ccagagacga
     1561 tcgttttgat ggccgccttc cttcagacca atcctccggg gagaggtgat ccgcgagggc
     1621 gacggagaga tcctggtgga gttttcgggc cttccctccg gggccgccag aaccggagcg
     1681 atgtccgaaa tctcttttga catgtcatgc aggattgagg tctggagagg ggctaatgga
     1741 aagatcccgg tgggcagcct gcggcgatct cccctgcacg gatacggtgg tctcatctcc
     1801 cgaggaggcg aggcaataga ccgggtattt ctgtgagctg atccatcgcc catcgggggc
     1861 cggtcggatt ttatggcagt aaatataata aattaggatt aaaagacggg ggaggccccc
     1921 cggcggatcg taccctagcc ggtcagcagg tccgcgagcc cctccacctc ctcaaccgca
     1981 aagtcgaact cgtcacctgc gagccccgcc ctgaagatct ccttcttctc ggggatcgcc
     2041 gccaccaccc tctcattatc cagaaactca agcatggcgg ctttccgctc ttcgtcgacc
     2101 cggttgagga cgaagtagag gggcttattt atcttcttgg agatctcggc gatcttcttc
     2161 gataagagga gggattcgta ggaggggtcc aggaccgcca gaatggcgtc gcatcccgcc
     2221 tcgatcccgc ggccgaagtg ctctatcccc gcctcggtgt cgacgatcac catctcctta
     2281 tcccccaccg cgaggttgtc gaggaacttt ctggagaggg cccccatggg acaggcgcac
     2341 ccctcaccga agtcgtggat cttcccgatg gcgagaagcc tgatcccatc cttctccgtc
     2401 gtcgcctcgg cagggatgga ggcgagggtc cacttttcgt cgaagaggga ggcctcctcg
     2461 cccccggcga aggaccttct catcttgccc atcatctcct tctttccccc gaagtggtcc
     2521 atgagggcgg acggaagctc catccccagc tgccggtgga ggccgtagtt cgactcgtcg
     2581 ctgtcgacca ccaggacgtt gaatcctctc tttgccatgc tcttcgccag gagcgccgca
     2641 acggtgctct tgccgctacc gccttttccg cataccaaaa ctttcatcct ttttcacatc
     2701 ccttatgata taacgatcat cagtcccctt cctccccccg gatctaaagg gggaggatga
     2761 tcctgcggcc gtcgatctcg aggatctcca ccttcatccc gtaggcatcc tctatattct
     2821 ctggggtcat cacttcttcg gggcggccgt aagccaccac gttccccccg gagggggcgt
     2881 ctctcctcaa agccggatga tgtactgccc catctcctcc ctttgggagg cgtcgcatca
     2941 ggaccacccg gtcggagtag gccagggcgt ggtttgggtc atggagggtc attatcaccg
     3001 tcaccccctc ggttgcggct atagaggtga cggtcttgag gaccctcacc tgatttttga
     3061 agtccaggta agacgtcggc tcgtccagga ggagaaacct cggctcctgg gcgagggacc
     3121 tggcgatcat caccatctgc ctctctccgc cgctgatctg ggtgtagggc cggttcgaga
     3181 gatggcccgc ccccaccagc tccagggctc ggcgggccac ctcgaagtcc ctggccccgg
     3241 gggacgagaa ggtggagacg tgggggagcc tcccggtgag gactatctcg aaggaggtga
     3301 agggaaagga agatcggtgc ccctggggga cgtatccgat aacctgcgcc agctccctcg
     3361 cccccatctg ggaggcgatc tttccgtcga tgaccaccga ccccgaggca ggcttcagga
     3421 ggcggtttat cgtcttgaga agggtggtct tgccgcagcc gttcggcccc aggagggtaa
     3481 ccttcgaccc ccgatctatg gagaggtcga tcccggcgag gatcggcatc ccgttgtagc
     3541 cgaagctcag atcccgcacc tctatgccgc tcattcccat ccccccgccc tggtccgtct
     3601 caggagatac gcaaagaagg gagcccccat gagggtggtg atgatcccta cggggacctc
     3661 aaatccgagg gcagcccggg ccaggtcgtc taccaggacc atgaaggtcg ccccgaaggt
     3721 tatgcagagg ggtataaggg tcctgtgatc cggcccgaag atcatccgga ggatatgggg
     3781 gactataaga cccaacaggc cgatgatccc tgccaccgcc acggccgagg aggaggcgag
     3841 ggcggcagag attacaaaaa tgcccttgta cctctcaggg tccatcccca ccgagcgggc
     3901 ctcctcgtct cccagggcga ggacgttcag cctccaacgg agggagagga tgcctagaca
     3961 ccccaggagg gcgaggggaa aagagctttc aagcttcgac cagctcgcag cgttcatgct
     4021 ccccatcatc cagtagacta tgctctggag agccctctcg tcgacggcta tctggacgat
     4081 ggccaggagg gacgagaaga ccgccgagac gatcactcct gataagacca gagagacgac
     4141 cggagtcctc ccttcggtcc tggccatgga gtaggcgaag gccacggcga ggaggctgaa
     4201 gatgaaagcg gaaagctgca ccggaatcca ggagaccacc cccagggaga gggcagcccc
     4261 gaaggcagcc ccggaggaga ggccgaggat gtaggggtcg acgaggggat ttcggaagat
     4321 cccctgaaag gcagtcccgg aggccgataa ggcggagccg accatcatcg ccagaaggac
     4381 cctggggagc cttacctgga atatcaccgt ctcgtagacg gaggggtgcc ccatatcaaa
     4441 cccgagcctc gataggatca cccctccgat ctcccatatc gttatcggaa aggtccctat
     4501 ggtcatcgat atgaggaagg cgggcagcgg cagtagtaga aagattatga taagggctct
     4561 tttgccaata aaagaagagt tatggaccgc gtccttcccc cgccgtccat aatcgatctt
     4621 catcaaggcc acccgatcta ggcggtagcg taacccggat agtggaccga gtacatatct
     4681 acaaagaagc cgtccagcct ctcatctatg ctcatctcgg cgaagagctc cggctggatg
     4741 ttcttggcga ggtgcatcag ggccatgggc atccggggag agaaggcgtc gaagaggaag
     4801 gggttctcga tctcgtagac ccgcccgttc ttgacggcgt tgatatcctt ccagcccgcg
     4861 aactccgtcc cgctgatgac gtcttcgggg tccagcttct cgttgtacca catgtatata
     4921 acgtcggggt tgaggctgat cagggtctcc agggggacgg tggggtgctc catccggctc
     4981 gcatcgttct cccagaagtt catcacgttg atccctccag cctggtatat caggtagtca
     5041 aagccgctct ccgtcccggc ggtccccagg acgtcgctcc acatccagta gaccttcggc
     5101 ctatctccgt cggctatccc ctctgtcacg gaggtgacct cagccacgta gctcttcatg
     5161 atggtttcca cctcggctgc ccggtcggtc ttcccggaga cgatccccat atcctccacc
     5221 tgcttataca tgtcatcgat ggaggttatg aagacggcat agacgggtat ccccagggtc
     5281 tccgttatcg ccttcaggtt ctcgtcctcg gcggatgtag cccagaggac caccagatcc
     5341 ggcccctcgg ccgccagggt ctcgaggctg acgaggccgg cgtcgaagcc tatcctcacc
     5401 ttatcgtcga acttcgggtc cacggcccgg aagaaatcct ctttaagctc cctcttccag
     5461 atgttgtcgc ctatggcgag gaccgtctcg ggatcagcta tcgaatagta ggtgtttagg
     5521 gcgttctcca ccagaaagac gaccttctgg cagggctggt ctatggtcac cgtccgttcc
     5581 aggttatcct ccagctccac cggggccccc agggcgggga tcgccagggc gaagcagagg
     5641 gcgatgcttg ccatatatcg atatatcttc aaaggaactc ctcctcaacg agcctctcct
     5701 gttggggaga ggaagcccgc agggcattct caccagaccc tctggattgg cgaattagga
     5761 tatataagtt ttgtatttgg tagcactaaa gacagatcgg atggatgaat tataacagta
     5821 ttgggcgaag aggtcgagga taggtcaaat ccttagcatt tcttggttga ttatatcggc
     5881 agccatcccg gcctcaggac gatgagagat taaggcatca tatccggcga tgacatcccc
     5941 tatttgagct acaatataga gcattcaccc tcataaatta ttatttgata atctatttat
     6001 agcaaaaaaa gacatttaaa gtagatccga tattactgat tatgagaaga gacccttcga
     6061 tcaggcctga gggatataag aggatcgaag aagggtaaga aggagaatag gttatgaaag
     6121 ggaagatgct ggcatcaatc ttggcgctat gcctgacggt ggggatatcg gcagccgatt
     6181 tcaccctcca catctacggc aacgccaaca tggacgacgt catcgacaag agagatatcg
     6241 aatacgcccg cggcatcatc gacgggacgg aaaggacgac ggatctcgcc gacgtcaacg
     6301 gcgatggcaa tgtcgacgaa gaggacataa ggctgacaga ggagatcatt gatggcgcgg
     6361 cgaccgagat cagggtgatc gactccgacg gtaacaccgt ctcagtaaag gtgccggtgg
     6421 agaggatagt catctacaac caccagtgcg cagagattct ccagatcctc ggggctgaag
     6481 atcaggtggc cggggtgaga gacaccttcg agacgcagga gaacaggttc ccggagatca
     6541 gcaagaagaa gaacatcgga agcggtgccg acgtggacat cgaggctatc ctcaccctag
     6601 atcctgacct gataatagcc tacaccttct atccaaccaa agaggacctg gaagcaaagc
     6661 tcccctcgga gatacccgtc ctgcggatcg actgcgccgg tgggggtccc tgcgggatcg
     6721 actccatcag ggacggcgta tcgatcctcg gatacctcct taatgcgagg gaggaggcgg
     6781 cacgatacca ggagtggcac gacaggtacg tcggtcccgt ggaggccaga acctccgcaa
     6841 tccctgaagg ggagagggtg agggtgtatc tagagtccac cccggagggg tccgagacga
     6901 tctcctccag gaccgccatc ggaaagaacc atcccgccgg aaacctcatt gagctggcgg
     6961 gcggggtgaa catcgccgca ggccagctcc ccctctacat ggacaccgac gaggagtacg
     7021 gcgagatcga gacggagtgg gtcctggaag agaaccccga ggtgatcgtc ggcagggcca
     7081 tgggagcggg ggttaggccc tacgagaacg aggacgactc cctcctgagg gcctactccg
     7141 atcagatcaa aggccttcct gggttcgacg gcgtcgacgc cgtaaagaac gaccgggtct
     7201 acatcatcac caacgatcac gccgtcaccc ccaactatcc gtcggcgctc ctcctcctgg
     7261 cgaagtggtt ctacccggag atcttcgacg acctcaaccc gagagaggcc cacctggagt
     7321 acctggagat gatggggatc tctaaggagg tcgccaacaa gaacacctac tactatccgg
     7381 cggtggcctg aatgaccccg aagcttggga tcgatagatt tgccatcgtc tttctcgccc
     7441 tggcgctggt ggcgatgccg gcgtccgcag ccctggggat cttcggcaac gccaacgtgg
     7501 atgataccat aaacgagctt gacatagatt acgtccgggg gatcatcgat gggagatacg
     7561 agaagaccga tctcgccgac gccaatcgag acggcgccat cgacgaggag gacatagccc
     7621 agatcgaggc gatcatcaac ggagaggaga aggtcctcga catcctcgac ggaaacgggg
     7681 atcccgtctc cgtccacaag cccgtggaga ggatcgtcgt cgagtacctg gacaacgcgg
     7741 agctcgtctc catcctcaac tcgaaggata aggtggtggg ggtcgactac gccgtcgcca
     7801 agagcgaggc acagttcccg gacctcgcca ggaggaagaa cgtcggccag atgaacgagc
     7861 cggactacga ggcggtcctc agcacggacc ccgacctcct cctcaccttc tcgcccatga
     7921 acatcaagtc gaaggccgat aaccttccgg gggtggacgt cgtcttcctc gggctctact
     7981 accccgacct gaaagatcca gcgaactcga agtacgtcga cggggtgagg aagctcggct
     8041 acatcctcga cgaggaggag agggcggagg agttcatccg ctggaacctc ggatgggtgg
     8101 aggagatcag atccagggcg gcaggagtct ccgaggagga gagggccaag gtcctcatct
     8161 ctgcctaccc ttatgcccac ctggacaccg ggaccttccg gacctactcc atgatcgaca
     8221 ccctcaccca gatggcggtc ctcaccgggg tcaaggtgat ggctgatgac ctccccgagt
     8281 tcttcggatc ttcctacaac atccaggtcg atccggagtg ggtgatagag tcggaccccg
     8341 acttcatata cctccatctg gtggcccaca cctacagcgg cctctccctg gaaccggcga
     8401 acggctacga tgccaccgac cctggggaga tcagggcagc aagggacgcc ttcaccagcc
     8461 ggccggagct cgccgagatg agggcggtga aggaaggaaa ggtctacctc gggagcggct
     8521 ccttcagaaa tgacgccacc ggcggcctca tcggcgccgc ctacatggca aaggccttct
     8581 tccccgagat cttcgccgac atcgaccccg aggcgatcca ccaggagtac atcgaaaggt
     8641 tcctgggtga agacttcgac ctcgatcaga gcggggtctt cctgtatccg ccgataggga
     8701 agggcgacgg ccggctggcg gggatccccg acaagtacgc cgagagcctg gcgccggctt
     8761 gatggagata tttagtccca tcaagcggcc cattcttttt ttccctgacg acatattaac
     8821 ctgtggccat acctcctgcc gagatcctga tccatcaaag agacggctcc caatggagcg
     8881 atctacaaac cctcgcataa gcctcctatg gatagatgga cctcctggcc taaagatccc
     8941 agaagcattc ccccgtcacc ccccttcaca agagatcccc tctcaccccg ccctcaagcc
     9001 gcaccagccg tcccccccgg atggccgcca cctcatcggc caagaagccc accacctcgg
     9061 gatggtggga gatgaggagg tagccgatcc ccctctccct ctggagatcc cggaggaggt
     9121 ggaggatctg ggcctggacg gagacgtcca gggccgaggt cggctcgtcg aggatcagca
     9181 cctccggctc caatatgagg gcccttgcca gggctatcct ctggttctgg cccccggaga
     9241 gctg
//
LOCUS       DASM01000123            2924 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_276884, whole genome shotgun sequence.
ACCESSION   DASM01000123 DASM01000000
VERSION     DASM01000123.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_276884"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    292..313
                     /estimated_length=22
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          708 a          814 c          906 g          474 t
ORIGIN      
        1 gccactgcta cgggatggcc gccacctccg ccctctactt caccgacccc tctctccggc
       61 ccgtcgcgaa gaagaccggc gagatgtcga aggaggaggc gagccagaac atagacatct
      121 accaccgcgc ccagatggtc ccggtgatga ggtcgatcct ctcggacgcg aacctctact
      181 tcgatagagg ttacgggtct agcagcagcg agagccaggc caggacctac gaagccgtga
      241 agcgctccct gaaagaggat cggagacctc tgatcatcag cttccggggg annnnnnnnn
      301 nnnnnnnnnn nnncctacaa gctggtggag gtggaggggg aggactacaa gcaggtctac
      361 atctacgact ccaacaccct cgtggcggag ctggagagta tgaagaagcc gatgcccgtg
      421 gccctcctct ggctcgacgg attcctctac tactcagttc agagcggtgg acaaaagtat
      481 gggtccaggg accccaccaa gatcgccgca aaccccgtct tcaggactat gcccccggag
      541 gaggcgggct ccctcctccc gggtctaaag gagatggcga aaagctggat cgaggcccta
      601 aagaaggagg ggggatttgc ggcggcggtc cgatgtcctg ccgacgccgt cttcaccgac
      661 cccgcggggc gaagggtggg gaccgtcggc ggccgggtca tagacgagat cccgggggcg
      721 gaggtgatgt cgtcgggcga ggtggagatc tacctcctcc cgatggacct cgcgtacagc
      781 ctggagatct cggggacagg cgccggcctt gtggacctcg atctcatcgc tcccgttgga
      841 gaggcttcag cgaaggtcgt ctccttcacc aacctctccg tggtatcggg cagaaagctc
      901 accggcgaaa tcgccgccgg ggccgagata gaggagctga aatcgggagc ctcgacgatg
      961 gagccgagca tttcgggggt cctcgacctg gaaggagaag gtgagacggg aaaggtggct
     1021 gagacgacgt ccggcttcat ggatgaggag aggacgggag gcgagggagg agatgggatg
     1081 ggagcggagg aggagctgat cttcgaacga aacaccctcg gcgccgtcca gaacggtccc
     1141 accagcccca ccaccttcgc cgtcgaccgg gagtggacgg tcaccgagat caagacctac
     1201 cactggaact atggccaggg cagggcgccg gggatgatcg gcctccagga cgagaggggg
     1261 atggtgttgg ggatgtggcc cgcctcgggg ctgccgggtt cgggcggcgt ccccgacgcc
     1321 tactggacgg cgcggccgaa cctggtcatc gggcctggcg attatacgat cctcgactca
     1381 gaccccgcca cctggtccca gaactcggat actggaggcg agggggtcgc ctgggtctac
     1441 ggggtcccat cggaggccgc aggcgagggg gccgatccgc ggagccggac ggtcggtgag
     1501 accgagaccg gatggggcct tcccgaatac ggcgagatcc tcatcgacgg accgcccggc
     1561 ggctgggagg gggtggtgcc aggggaggcg atataaaggg gaggatttcg aaaacccctc
     1621 taaatcacaa aatccagatc ctccctaatt aaagggtatt taagtgatga cgctcgaccg
     1681 gggtttaatc ctcttcagcc aatggaataa ccgatgtaac agcaccttct ctggtaaaca
     1741 tggatcttcg gttgaaccag accaccagag cagcaacaat cacaaacagc caggaatcat
     1801 aaggctgagc atccggccat aggggattga tcaactgaag gtggaaaagc gcggggagga
     1861 ggatactgcc gcgcgaattg ttgaagagcg gtgtcacgat gacgcttagg gctacggtgc
     1921 ctacaaagaa cggcgtcaac gaccaggcgc tttgcggggt gccgctgagc ataaaagcag
     1981 gaagatgcca gaacgcccag ataaaaccca gcaccagggc tgcccagatg ggcgccatac
     2041 gacgctggag cagcggtagc gccagtccac gccagccaac ttcctcgatt ggccccttga
     2101 tggccatgag aagcagcgcc aagagataag tcgccaccga tgaaaatgga aacagcgttt
     2161 catacgcgcc ttccttgaac agggccgaga tgtagaagac caaaggtacg accaggatca
     2221 acatgagata ccagtaaatc gacgtccgcc acaacagcaa tcgggagaga aaccgtcgta
     2281 tcccgccaaa accatgccga tagtgaacca aaaccaaggc tgcaatggct ggtgcataaa
     2341 ccgcaaggta aaacagcggg tgttcgcccg taagattccc gaagaaccga accatcggct
     2401 cagaagcaaa tatgtaaagc ccgaggatgc cccaggcgat gagaaaggcc atagtaaaga
     2461 acgggatgag atcgcgttca cgcatatcaa aacctcctta tgtgattaat attgctactt
     2521 ttgtgaaggc taaaaaactg ttggtgtgcg acctttgccc acaacaattc tgatagaaat
     2581 tgtagaatca tcatcctgat tctcgcgata gtctggggac ttatagggct agcgggagac
     2641 ccctctaccc gccgccgata tgaccctatt taaagaggcc tggggggcga accggcggaa
     2701 agccgcccga aaagtattag gcggatcatc gacgaagcct cgaccttcag caggctcagg
     2761 ccaacgatcc caagcgagat tccgagatga aatcgatcgt cataaaaggt gcaagagagc
     2821 acaacttaaa agagatcacc atcgagcttc cgcgagaccg gctcatcgtc atcaccgggg
     2881 tctccggctc ggggaagtcc accctcgcct tcgacaccat ctac
//
LOCUS       DASM01000124            9081 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_105339, whole genome shotgun sequence.
ACCESSION   DASM01000124 DASM01000000
VERSION     DASM01000124.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9081)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9081)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9081
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_105339"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    7404..7422
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1653 a         2865 c         2896 g         1644 t
ORIGIN      
        1 agaacctgga agctgcgatg aagtacttca tcttcggatc ggtctcatcg gcgatgatgc
       61 tcttcggctt ctccctccta tacggcctct cgggaacgac gaggctagcg gagatcgctg
      121 ccgcctccgt ggagacgatg ggacctgcga ccctcgtcgc cctcctcttc gtcgtggcgg
      181 gcttcgggtt caagatggcc ctagtcccct tccacatgtg ggctcccgac acctacgagg
      241 gggcgccgac gatcgtgacg gccttccttg cggcgggctc gaagaagatg gggtttgtcg
      301 ccgccttcaa ggtcatcttc atcgccctga tagccctccg gtttgagtgg tacttggcct
      361 tcgccatcct ggtggcgata accatgaccc tcgggaacgt cgtggccatc tggcagaaga
      421 gcgtcaagag gatgctcgcc tactccagcg tcgcctacgc cgggtacatc gccatcgcct
      481 tcgtcgtcgt cggagccgca gagcacggcg gcctcgacct ggcggtggcg cagaacggcc
      541 tcgcgagcgg ccttatgctc atcctcggcc acgccttcat gaagaccggg gccttcatcg
      601 ccgccgccgt ggtcgggtac atggccctct ccgaggggag gaagaaccct gacgacctgg
      661 agcagtatgc gggcctcggc cgaagggcgc cgataaccgc cttctgcatg ctgatcttcc
      721 tcttctcgct atcggggata cccccgacgg cggggttcgt cgggaagttc atgctctggg
      781 gatcagcgat cgactccggc ctcgtctggc tggcggtgct attcgccctc aacagcgctc
      841 tctccctgta ctattacctg agggtgatca tgtacatgta cgtcagagag cccgcgggcg
      901 ggaagatcgt ggagccgaag gggtacgtcc tggccatggt cgtcgccctg gtggtcgtca
      961 tctggatcgg cgtcttcccc caggccttcg tcgacctcgc ctacagtgca gcaggggttc
     1021 tgctgcactg aaaactttct tttttctcgc accattttga cttccgatcg tttttaaagc
     1081 cgagattgct cccgattaga accccgtctc ttcgatccaa aatagaagtc ccaaaaggat
     1141 cagagccgtc gggatcacca ccatccaggg gttcaccccc aggatcttgg ggatggtctt
     1201 atccccgaac tcgcccctcc tcagcacccg ctcttcgagg ctggggtacc tccaggcgaa
     1261 gatgccggcc ccgaggagga tcccgccaac gccacccagg agggcgtcga tcgacccctg
     1321 gccgacggcc ccggccgccg tccccgggca gtagccgagg aggccgaagc cgacgccgaa
     1381 gatgaggccg cccagggcag aggagccgag ggagccggat ttggggtgga ggctcgcgag
     1441 gcccaggctt ttgaggaggt ggaccccgac catgccgacg atcaccgccg aggccatcac
     1501 cttgaagacg gtgaagtcgg cgaggaggag ctggcctacg atgacctcgt accgggtgac
     1561 cccccccttc tggaggagga agccgaatcc gatcccgatg aataggccga tggcgagctg
     1621 ggcccggtga tttgatcgca gatcttcaat tgccaaatcc accaccgcct cagaccccgg
     1681 ccccgaagag gagcatagcc gtggccaccc cgccgatgaa gaagcacgcc accgccagcc
     1741 aggagctggc ggcgagctgg agggtcccgc tgatcccgtg gccgctggtg cagcccccag
     1801 cccaccgggc accgaggcct aggaggacgc ctccgacgaa ggcagcggcg atcctcagga
     1861 ggggatcggg gccgaaccga gtcgcccaga ggtccgggac cgcctccacc cggaaggacc
     1921 ccgagaggat cgccgagatg aatgccccga cgacgatgcc gaagacgagg accacctccc
     1981 agtcgacgac cggctcaaac ttccgatagt aggccttggc ctccaccttc gatcctcgaa
     2041 acgccctctc gatcatcccg ctccccctgg cgtaagcgga tgaggtgcct atgggccggt
     2101 cagagaggag gaaggccagg gtgttcaagg cccctatccc agcacctacg acgtaggggg
     2161 accacagatc catctcgaat tgctccatca tacgatctcc tcggtctacc tacctagagg
     2221 cgggaccttc gggggaaggg ttccaccgac caggcccgat cctctggatg atcaatttaa
     2281 gtcgtcacat ctttataaag ctgatacgaa gacggggacg gatcatcgtt ggagatgaga
     2341 agatggatgg aagactatct ttgaggctgc tcatcaccct cgcggcgatg gcggcgacca
     2401 tctcagccct tcccggaggg ctcggctacg ccgacccggg gaaggcggaa gggctggtgg
     2461 tcctcctcac cgactacggc acctcaggct tccgcgtcgg agccctggag gggtcgatat
     2521 attcggagaa cccccaggcc cggatctcca ccataaccca tcaggtctca gccttcaacg
     2581 tcgccgaggg gtcatacctc ctcgcgaaag cagccaggta ttatccgccg gggacggtct
     2641 ttgtggcgga ggtgaacccc ggggtgggga cggatgagcg gtccatcgtc gtcgagacgg
     2701 aggacggcaa gctcttcgta ggccccgaca acggcctctt caccgacgtc atggaggatc
     2761 tgggggtcgt tagggtttgg gaggtcagag gcctgaacct cacccgttcc gggggtgatc
     2821 ccgtcacctt caacggcgtc gaggtctacg gtcctgttgg ggctattctg gcggccgggg
     2881 ccgatcccgc cgggctagga cccgagctct ccgatcccgt gaggctcgag cggcagaggg
     2941 cgggggtcga gggcgatgag atggtggggg ccgtcgccta catcgacccc tgggggaacc
     3001 tggtcaccaa catccccgag gagctcatcg gggggacgga tatcggtccc ggagatcggg
     3061 tcgagatctc cgtcaacggc tcgaggatcg atgcgctctt tggcacgacc tacgccgacg
     3121 tccccgtcgg ggagtgggtc gtcctcgtcg gcatcctcgg tcggctggag atagcggtga
     3181 acatggggag cgctgccggg gccctggggg tcgccgaggg gtccgccgtc agggtccggg
     3241 agatctgagg gctcagaggt agaatagatc gaccatcagg gctgcgacga ggatgaagat
     3301 caccgtcatg atccccccgg cccggatgta gtcggagctt gagtaccctc cgggggccat
     3361 caggagggcg tnnacctggt gggtggggag gatgaaggag ttggaggcag agatccccac
     3421 cagcagggcg agggccctcg gatcgatcgc catatccctc gccatgatca tcgccagggg
     3481 gacgaggagg accgtggcgg cgacgttgga gattgtgagg gagaggaggg tggcgagggc
     3541 tgccaccgcc aggaggacga ggatcgtcgg ggctccccgg aggtggtcca tcatgctctg
     3601 ggcgatgagg cccgcagccc ccgtctcctc catggcgatc cccaggggta tcagccccgc
     3661 caggaggacg atcgtcctcc agtcgatcgc cccgtaggcc tcgtcgatgg agatcacctt
     3721 caggaggacc atggcgaggg ctccggtgag gagggcgatc gagagctgaa cccctgtgag
     3781 ggagagggcg acagctccga ggaagcatag ggttgccggg atcgccttcg acgggtcgag
     3841 gacccccgcc tccaccggcg tcaggaggac gaagttctga tcggatccga tctttcggat
     3901 tctgtcccag aggccgtgga tgacgaaggt gtccccgggg ctgaggggcc tgtcgtcgaa
     3961 ctccttcctc tcctccctcg taccgctgag aaggatgatc ggctctacgc cgtagttcct
     4021 ccggatcgag atctcccggg gggtcttgcc gacgatgggg gcccggggcc tgacgacgat
     4081 ctccgcaaag cccgcctccg cctggctgag gaggtcgcag aacctcgatt cgtcggtgat
     4141 cttccagagg tcgtgttcgg cggcgaaccg ctctacgtcc tcgggctccc cgaggagcgc
     4201 cagctcctgc cccgccgcga acctcgtcct ccgccagggg gagtaggtca ccatcttccc
     4261 ctcgacgatg gcgaggaggg ttatgccgta atttcgccgg agccggaggt cttcccgggc
     4321 cttatcgacg aggtcgctct tccaggatac gaagtagtag ctgacgatgg aagggagcct
     4381 ccaggtctcg atcaggtcct cctgagatct gaaatcgtcc tctgagagcc ctctattcgg
     4441 gaggaggcgg tctccgaaga tcatgaagta ccctatcccc gatccgagga ggacgatccc
     4501 gatcggcgtc acgctgaaga tcccgaaggg ctcctccccc ccctgcctca agaggtcgtt
     4561 gaggatgatg agggggcttg agcccaccat gctgagggtc ccgccgagga tcgccgcaaa
     4621 gcccatcggc atcagaagtc gggatgccgg ggtcccggtg ctcttggata tcctcaggat
     4681 ggcggggagg aagagggccg ccgatccgag gttctgcatg aaggcggaga tcatcccgac
     4741 caccgacgag gtgacgccga ggagcctcct ctcgtcggat ccggctactc gcaagaccgc
     4801 ccggctgatc ctgttgacga cccccgccct ctccatcccc ttccccagga tcattatcgc
     4861 tcccatggag acgacggcgt tgctcgcgag ccccgatagg gcctggggcg ggctgacgag
     4921 gccggtcaag gcgaggatga cgacgacgat gatcgccacc agatccaccc tcacggcctc
     4981 ggtgacgaag aggaggatgg cgaccgccag cacgagaaat gtcagctcca tctccagaga
     5041 gatgccccag atcatcagca acggacctcc tgcgacctga tatcccttgg ttttcagtcc
     5101 ttaatagaag atggcatcaa tatatagatc tcatcctctg ggatcccggt gggcgagaga
     5161 ccgcccccca gggcctcttc attggaggct ccagcccctc ccgtcacccc ggctcggggc
     5221 tcagaagagg gatatgatcc ccatcaccga agccgtgact atgcctcctg cgacgatcac
     5281 ccccgcggcg atggcggaga aggcctctct gaacctgagg ccgaagatga aggcgatggc
     5341 ggagccggtc caggcccccg tcaccgggag gggaacggcg acgaagaggg cgagggcgaa
     5401 aaggccgtac ctccggtgcc tttccgcgtg cctcttataa gtcctggtga agagccagga
     5461 gaagaaggcg tggccgggcc ggtatcgcat cagccagcgg gataccggtt ccaggtagag
     5521 gaggaggggg acgaccggag ccaggtttcc ggcgaccgat atggcgtagg cctccaaggg
     5581 tgagaggccg tatacggcta tccccagggg tatcgacccc cggagctcga tgaccggggc
     5641 catcgcgagg acgaaggtgg tgagccaccc tggcatcgag ctgaagaact ctatcatagg
     5701 aaaccctcgg gctgccccgg gactatcccc ctctcgggat cggcctcgct ctttcgcgcc
     5761 ggcggggcgg agatcccggg cgttcgggcc tcactccccg atcgatcccc tgatcctgac
     5821 cgaaaggacc gactcgtcct tgggggatgt gacggagacc gtcaggtcca gggggattct
     5881 gccgagggct accttcagcg ggcggccttc gctgcctctc acctccaccc cgatcccctc
     5941 cgccatcttg gacaaggcct gggcgatctc ccgaaggtcg gggaggctga caaccacggg
     6001 ggtatcccgg tcgccgccca cggtggcaga tatcggagaa tctatcgcca ccgccacatt
     6061 cagggcgccc tccttccccg ggcccttcac ctctgctaca atcggatgcc tttcggcatc
     6121 ttcgctcccc tgaactaccg ccttagcatc cgcaatcact ttcagagata ccatgaaatt
     6181 ccacctggaa gctaattacg gcatcctctc atataaatga atgcgatgag aacggcgtcg
     6241 gccgccggtc cggcatcagg agcttatgat gaagtttatg ggcccacgcc tcctccatca
     6301 ggttttttgc catgggagat ggccgcagat ggtcaggatt cttccttggt cggctggacc
     6361 cctgcccccc cccgggcttg aggtctaaag tcccgccctc agaacgttct cagcccccgg
     6421 ggtggccttg atgaaggccc agtcggatct ggttccggtg tcgacgccgt tggggtagcg
     6481 ggaccaggag aagtcgtttc cctcctcgtc ggtgagggag tgagtccgat ccacctcagc
     6541 ccaggagccg gtcctcagga cgacggtcgc gttattcccg tggatccagc gccgatcccc
     6601 ctcggcgacg caatagcccc ctggcgggat ggacgtcccc ttcggtatgg gtatcacccc
     6661 cgtccagatc cccggggcta cgagggtcac ctcgatggcg acgctccact ggcctaaatc
     6721 caccggctct tccccggagt tgtagagctc cacccacaag atcccctccc ccgcggggtt
     6781 cagctccacc tcgtttacga cgaccgatcc cgatccgatg gcggcgagga ccgcaaaaga
     6841 tgctaagaac gatggcaaga ttagtctcat gataaccccc acatgatcca tcctgagatc
     6901 cggggcgcag agccgtcccg gccttctgga tgagatcgat cttcggataa ataaccatat
     6961 cgatcggcgc gatgggaggc tgtccctctg cccccccacc gaggccgggg gccgatcccg
     7021 ttaccacccc gccgtcggct ttggcctcgg atcaggagtt gggggcgttc ttcgtcccga
     7081 tgccaaagac ccagtcggac ctctggtcgg agtcaaaccc gttgggatgc cgaaagttgg
     7141 tgaagaagtt attgctgtgg tcggagagaa ggggagtctc gtcgactaca gccccctcat
     7201 cggtcttgag gatcaccgtc ccggtcccgg tattgtggat ccaatgtcgg tccccctcgg
     7261 cgacgaagaa gctgcccggg gccatcaccg tatcctgagg tatcttcatg accccggtcc
     7321 aggatggact ctggtcgacg atccagacgg accagcggct cacgtccacc tcttctatgc
     7381 cgttgttgta aagctccacc cacnnnnnnn nnnnnnnnnn nncagctcca cctcgttgat
     7441 gacgacgccg ccaagacatg ccatcgtcga cgccagaact cccagcaggg aagccaggag
     7501 cctcacccca tctctcttca ttacgaaaac ccccacacta atccgatatc ctcctgccct
     7561 agggcagact gccggagatg ccgggctgaa agatctccag ataaataatc gtatcgatcg
     7621 atggagggag aggcgagagg gccccctccc tccgccccga agccgatgga tatgatgaag
     7681 gtccggacct cctcggtcct gagggccggg tccgaaccct caaaaatcag atcccatagc
     7741 ttccgtctcc atcgggcccc cgatcagctg aggacgtttt cggccctggg ggtggccttc
     7801 atcagcttcc agtcggacct ctggtcggtg tcgaggccgt tggggtagcg gccgtaggtg
     7861 aagtcgctgt cgccgtcgtc gaccatctta tgggtctcat cgaccttcat cccgatcgcg
     7921 tccaccaggg ccacggagcc ctcatcctct cctctccact gaggatcgcc ctcggcgatg
     7981 tagtaccctt tcggagagat gaccgtcccc tgggggatga cgatcggtcc ggcccagggg
     8041 aggttgtata tggtgacgac ccagccggag atctcgaccg cctcgtcccc tccattataa
     8101 agctccaccc atctggtggc gttcccgggc ggattgatct ccacctcgtt tatcactatc
     8161 gatcctgcgc cggtcgccga gagggcgacg gctatgtaga gagatatcaa tatccctgac
     8221 ctaactcgca tggttcaaat ccccccgnng gggcctcccc caagagagct tctgagtaga
     8281 taacggtatc gatggcctct ccagatcgcc gtcctccgat ctctctctcc cgatgggcag
     8341 aggccctcaa ggggtctgaa gccgcctccc ccccgccggg gtcggggttt attacctcgg
     8401 ggctcctgga ggacccctca tggagtattc ggaagggcgc cttgctaggg tcttcgttat
     8461 caagatggag gagggggagg agatgatcgg atctctcgcg agattcctgg cggagaaggg
     8521 ggtcgagagc gggatcgtcc acttcctggg ggccctccgg gaggggcggc tgatcatggg
     8581 gccgagggag gcgagggtcc ctcccgggcc gcccttcgtc gaggatctgg aaggcggctg
     8641 ggagatcttc ggccttggga ccgtctaccc cggtgaaggg ggggcgcctt ccatccacat
     8701 ccacgcctcg gtggggcgcg ccggtcgggg tctgaccggc tgcctgaggg agagagccca
     8761 ggcctacctc gtcgtcgagg cggtcgtctt cgaggttgtc ggcctccggg cgagaaggtc
     8821 cgtcgacgag aggacggggc tctggctgcc tgaactggag gagagggtgt agatgggggg
     8881 ttgggcgagg cggcttggaa agagagactg gaaatggcac agatgagggg gctatgacga
     8941 ggcgcaagag gggcgggcgg aggcgtatga agcagcgggg tccatcgcct ctgccgatat
     9001 cgatggagga ggcgaggcgg gacggcatcg accgcttcga cgtcgtccta gtctcggggg
     9061 acgggtacgt cgaccacccc t
//
LOCUS       DASM01000125           31184 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_49830, whole genome shotgun sequence.
ACCESSION   DASM01000125 DASM01000000
VERSION     DASM01000125.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 31184)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 31184)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..31184
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_49830"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    21692..21724
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5789 a         9760 c         9706 g         5896 t
ORIGIN      
        1 gtagccgagg agctcgtcga agggctcgta atcgacggtg tagtaggtct cgccgccgta
       61 gatgatggcg tcgttggagg ctcccatgca ctttgtggcc ttgcccacga ccggcgctat
      121 cggggccctc ccccagccgt tccttatcgt cctgacgtcg aagcccaggg tgaagagctt
      181 atgcaggccg gtctcgacga cccgggccga gacctggacc agccccgcca ccgagtcggt
      241 gggggcgacg gcgaggcgga ggtcggaggg gtcgatcccg caggcctcgg cgatcttgtc
      301 ggcggccgcc tcgtcgggga gggagtcgga ctccaggaag aggacgccga cgtcggagta
      361 ctcctcaaac tccagggcgt cgtagagctc cttcgtcttc ctcgccagag ccctggcggg
      421 acccgaggcc atggcgaagt acttgccggc cttcacctgc cacatggcgc actgggaggc
      481 catgcaggag atggcggggt ggtcggtcgc cacctggacc cccgggatgg tgatcccgtc
      541 gatctcgatg ggggtcggct ggatgtcggc gaggtcggcc atgcagatct tggaaaggta
      601 gatccccgcc ccgagccccc ccttcgcctc caccccggcg tcggctacca ccgtcccgtt
      661 atccagctct accaccccca cctggatctc gtcggcgtag tccagcatct cctcgaagac
      721 ctctaatcca agctcgttca gcggctgcag agttctcacc tggaaggaga agaggctgga
      781 gccgatttaa atttgccgat gccaaacctt ttcaccgggg cggccaaacc ccggctatgt
      841 tcgaagcgga agaggagtcg gtgatcctgg agatcgtctg cgggatcctg gacgtatccc
      901 ccgaagatat gatggagatc ggcgagaggg cgaagaggat gataaccttc gaccccgacc
      961 tcgagaaaga agaggaggcg gtcgggaggg tccgggacgc tgtgagggat atgtcgtcgg
     1021 cggaggcggt ggtggcaggg gtcttcatat ccggcctcct ccggtgcagc ctgagagaac
     1081 agtacctccg ggccggagcc gaatccgagg ggtgggacgt cgaaaggaag gggggtgaag
     1141 ggggcgaggg gcggcccggc ggagagggaa gattagaaca ataagagcag gaagaagggc
     1201 ggaagcggcg gaggctggtt gtggggggtt tcagcccgcc atccccagtc cggcggggga
     1261 ccaggggagc cgcttcctct ccggcctggg ggacctgtcc ctcgtcctgt ccccatcccc
     1321 gacggggtcg gccctcatgg ggaagagtcc gaagactggg ggtccggcga tcggtcccac
     1381 cccaggtgct gctataaatt cagcttccat cgacggcatc atctccgcgc cgcagccgcc
     1441 gccacagcct ccgccgcagc tgccatcacc acagccgtcg ccaccgcatc cgcaaccccc
     1501 atcctcagat tctccgccag ccggggcatc gcagtcgtag tcgtagccgc tctcgtcgcc
     1561 gaccctctcg taactggcct tctccaggtc catcctcacc gggaggtctc cggcgaggac
     1621 cggctccctc aagtcggcga ggaggagctc cagggtcacc aatcccagtc ttgccatttt
     1681 gtcgtacatc tcctggaccg tcttcgacga tcgcctattt tatatacggc aattaaagcg
     1741 agcttactat atataggtta ctaaggccat attagatgga cagatctgac caggccagga
     1801 ggaaggcgga gccttccaaa agcccccggc ttaggagcca gaggatggag gatctggccc
     1861 ccaccggggg tagggatggg cggggggccc ctcgtctccg tctaccaaag ccggatcaca
     1921 aagcgccggg gagaggcttg aagcgccagg ccggcctgat ggtagggatc ccgcccccga
     1981 ccgggggagg cgggggagat cccggaggag gtcgatcccg ccgatcacct caccacgagg
     2041 acggggaccg tcgcccggta gaggacgttc tttacgacgc tcccgatgat gaacttggtg
     2101 acaccgctct gaccgtggcc tcccatgacg atcatatcga cccccctctc cttggcgagc
     2161 tcgatgatgg agttggcggg aaccccggtg gtgacgaccg tctcgacggg gaccccggcc
     2221 ccaccggcca tcctctcgag atccctcacc gccccctctc cggtggcctg gagcttcctc
     2281 tccagatcct ccttgtcggc cccaaagagc ctcacggcgt ttaatatcgc aacctcgtcg
     2341 actacgtaaa ttccgatgac ccccgccccc gtcgccttgg ccaggtctat ccccttcttc
     2401 accgccaccg aggaggcggc ggagccgtct gttgccacga gaatcatttc ttccatttat
     2461 ataacacctc tcaaatacat ataacctatc cactaccatc tgcacttttg atttatggac
     2521 tattgatata aatacgctac ttccggattc ggaccgcgcc gggaggaaga ccggcgttga
     2581 ggccgggatg gcgccttccg cccctcccga tagccccgac ctcgagggaa ggaggggtgg
     2641 aggggtggag gggaggatgg gtaaagggag agaatctccg ggaaggggga gataagataa
     2701 tacgcccgga gggcgagcat cgacccagga ggaaggagat cttgcgaaag ctggatagcg
     2761 tcgtccagga gctggagggc cgggggctga agttccggca gaccgcctct cttcggatcg
     2821 ggtacgtggc ggacgtctta tttaaaaagg agcgggttat cgtcctcgac acccggaacg
     2881 cggacccctt cgccgtccgg aagctcgcgg cggcggggta taaggtcttc gtcatccccg
     2941 aggggaagct caccgacgat cagatcaggg ggttctgcga cgaggtggag agggggatcg
     3001 ggaggtaggg agaggggcgg gggctctcgc aagaaagatt ttggagatca aacgaccccg
     3061 gcgctgtgat acggcccccg caccgcccgg cgcagtggcg ggggggcgga gggttccagg
     3121 aaggtcatcg acgcgccggt taataaaata taatcaagaa tatattccgg gcggcgaaaa
     3181 cacccgcccg gccgctcccg ccgggcggcg agatcgccta gctgcccccg accatcatcc
     3241 atccccgcag ccctcccgcc cggcgcggaa gcgctcgaag gcgctgtacg catgatgggc
     3301 cagcaagagg aggcgcggtg ggtgccggga gggcgtcgat ctgcctatcc gtcgtctcga
     3361 tctgccgcga gaagaaggtc ttctcgtggc cggtcatcgc ctccgaaagc cgcctgccaa
     3421 aggcgagcat accatcggcg cggaccacca tcgaagggtg gcgggagaga tcgccgaaga
     3481 tatatagagc tattaaaatt taattaaaaa tatatcccag gcggcgaaag accgccctgc
     3541 ccgccagcct ctcgcgccct ccccgtcgat ttacgccctt ctaccgaccc gacatcggcc
     3601 cccacatcct ccccctttcc ttctcgatcc cgaagaacga acgcccggcg cggatgcgcc
     3661 gggcggcgca gcgcccagga taggccagaa gagaagaccc ccgaagccag ggcggcgccg
     3721 tcaccgggca gcgtcctcga cgatggcgat ctcctcctcc gataggccgt agagctcgta
     3781 gacgagggcg tcgatctgcc gatcggtcgc ctcaatctgc cgcgagagga gggtcttctc
     3841 gtggccggtc ttcgcatcag catgccgctt gttcaggtcg agcatattct cgacgaggcc
     3901 caccatctta tcgtggcggg cgacatcctc ggggtcggag aagtcgatag tgcggatggg
     3961 gagctgccct acatttacag tttttacttc agcgaatgct ttcccaactt caggaataat
     4021 tttttgataa taatacgtca ataatctcga attgaataaa cccagaatat actttaaatt
     4081 gatttggctt atatctttct tcatcaaaat taaatgagta gaatgtcgag tgatatgatg
     4141 ttcatcatca aaggtaccaa tcagtcgatc tgcagtttgc cttaatatta ttttatctgc
     4201 gtgaaaaatg gccggatccc ttagggcaaa atcgatcttt ttttgacgcg tatcgagtga
     4261 cgttatgtca attttatccc ttagtgatgg atcatagcat atatataatc cattccaata
     4321 tatagcatat cgagatatgt tttttccctc aagtattttt acatatctat aatctacttt
     4381 ttcttttgat agtaaaattt ttgccatatt tccagtattc gcaccattat aaacagagca
     4441 tatttcagaa agatttgtat ttgagttttt tatctttact attagatcaa aatgttcttt
     4501 attcattatg aaaaaattga aattttgttg ttccctaaaa tctttttgtt ccatcttgac
     4561 ataagcttgg ctgtcagtgc taaagaaatg gacatcatca acaacagcac cgttagttat
     4621 attcgacgaa tatgtttcat caaagttgga ttttattagc acaagtatta cattaccacc
     4681 cgtttctgcg ccgttgaata ccgaatctct gatttcaaca agagtatcca aataacaaga
     4741 atctaagatg attttcctca attttttaaa gtaattatta tccataaatg cccgaggaac
     4801 tataaacccc aatcgtgatc cataagacaa tagttttata cctttttcaa taaataaaga
     4861 aaaataattt attttatatt cagctgattt atacctggca attgcataat ttttaacgtc
     4921 caaacttaca tgactatttt gcttgccgga aatcgtaata tacggcggat tcccgatcac
     4981 cgcgtcgaac ccgccccggc ccatgatctc ggggaactcg gcctcccagt cgaaggcgtt
     5041 caccctgtac atctcctcct cgtcgaagag ggaggtctgc cggtcgtaga agtcggggcc
     5101 gatgagggag tttccacatt tgatgttctc gccgaggtcg gggagggccc gctccttgaa
     5161 gagggtcagc tgctgggaga gggtctcccg gttctccccc tccaggacct tcagctggag
     5221 ggagagcttc gtcacctcca ccgccgcaag gtcgatgtcg acgccataga tgttattgag
     5281 gaggatccgc ttcctctcgg cggtcgcaag cctccactcg ccgcccgcgg cctggaagat
     5341 ggggaaggac ccggcccgct ggccgttggt ggcgctgcgg ctcgcggcgg cgctgctctt
     5401 cctcggccgc ccccgcctct tcgccgcccc ggcggcgttc ccgacggccc cagccccagc
     5461 cccggccccg gcgttggccc cagcgagccc tccgggcggg aggagggcca ggacctccct
     5521 ggagctctcg gccttcccgg ccgcaaggtg cgggacgagg tggcgggtgt accattcgag
     5581 gtgccagtcc agaagccgct ggtatgcgcc gatgagaaac gagccggagc cacaggcggg
     5641 gtcgaggatt ctcaggtcgg agacggtggt gggggtctgc cccgcgagga gctttccgac
     5701 ggtgttttcg acgatgtagt cgacgatgta cgtcggggtg tagtagacgc cccccgcctt
     5761 ccggacctcg ggcttgtact ccacctcgac cccgccatcc tccccgaggc tgatcacctt
     5821 tccgaggaac tgctcgtaga cgtggccgag gatctccgcc gggatcaccg agaaccggta
     5881 ggggctcctt ggaggatata ggttctcgat catctcagcc agggccgaat cgtcgatctt
     5941 gagggcgagg gccagccggt ccggctcgcc gatccggctg gggtcgtcgc cgaagtggaa
     6001 gaggccggag ttgtacctct cgtccgcctc cttgaagagc tcgacgagcc tcgcatatac
     6061 cgccatctcc cccttcagga gccccttcag ctggccgaac ctctcgatcc cccggtcctc
     6121 gcagatccgg aggaagacta tcctgtcgat ggtgttctgg accgaggagt tcagctctct
     6181 gatggtgagg tcggggttgt tcctgtggac gttcttggcc aggatctccc gccaggagtc
     6241 gatctcccgg aggaaggcct cgtcgacggc gatcgttccc ctcgccttct tcttcgactc
     6301 gatgtacctc tcaagagacc cgcccaggac cgcctcccgg gagaagaggg tggcgatctc
     6361 gtcccagtga tcgacgtact tgtcgtaggt gaagtggcag aggagcccga ccctcggcga
     6421 gtcggtgttt gcaggctcga tcctgcagtc gtagatcgaa aactcctcga agtcggtgag
     6481 aaggcttatc ggaagccccg ccgaccaggc gtagctccgg agctggaacg ccgctctgac
     6541 gtcattctcc aggttgacgg agggcttctt cgcctcgacg aagaacttcc gggctccgcc
     6601 gatccgaaag gcgtagtcgg gggccttcgt cgaacccctc accttcaccg agtcctcgtg
     6661 gaccacatcc cggtaaagct cggagctgcc ggcggtgttc gccacgtccc agccgagggc
     6721 cttgaagaag gggtcgatga agtcgcgccg gagcttcgtc tcgttgtacc gacctgaccg
     6781 atactcgtcc aggttccgct caaaccgctc caccagctcg acgatctccc tcggcgccgc
     6841 caacttctct caagcctcct cgaatcttca atggtgaaat ggtacttacc ctttgccttt
     6901 cgggttgggc cgccccagcg ccgcaagatg gatatgatga taagaataaa aatctaatga
     6961 attaaaaccc tctcggggcg accttcgatc ccgacccatc ggcggcccct catctgatcg
     7021 gccccggagg ctgccggagg ccgccccagc ctccgcgccg cagggaatat attcagaaaa
     7081 gtataaaata tcacccgata aaatattgac ggggctttcg ccccctcacc ctctcctcac
     7141 cgtctgctcc cttcccggcc ccaccgagac gatggtgatc ttcaccctga gaagcgcctc
     7201 gatcctctcc acgtaatccc ggacggcttt cggcagcgcc tcgtacgact tcgccccgct
     7261 gatatcctct gtccagcctt tgacctcctc gtatatgggg cgacaccttg cgaactcaac
     7321 ggtgctctcc ggcgggtaat tgagggtctt gccatcgagc tcgtagccgg tgcagatctt
     7381 gatggggtcg aggcccccca ggacgtccag cttggtgatg gcaagctcgg tgtagccgtt
     7441 gaggtacatc gacttcttgg ccaggacggc gtcaaaccac ccgcacctcc gggggcggcc
     7501 ggtggtggtg ccgaactcct tccccacctt ggataggtgg gctccgacct cgtctttcag
     7561 ctccgtgggc atcggcccct cccccacccg ggtcatgtag gccttgacga tcccgacgac
     7621 gtcgtcgacc cgggcggggc cgacgcccag gttggcacac gcgctcccgg cggtggtgaa
     7681 agacgaggtg acgaacttct gggtcccgtg gatgacgtcg aggaaggcgc cttgcgcccc
     7741 ctccgccagg acctttttgc cggcgtcgag ggctacgttt atctcgtagg agacgtcggc
     7801 gacgttctcc ttcagagcct cgccgatctc gaggtactcg ccgaggagga cggggtcccg
     7861 gaccactgag gggtcgccgc ccatctctgc gatcgccttc tccctggcgg gtccgatctc
     7921 cgccagcttc gcctccagga gggcggggtc ggtgaggtcc ttcatctgga cctcgtcgcg
     7981 gctgaactta tcgatgtagg cgtagccgat ccccctcttc gtcgtcccga tcttctcctt
     8041 tctgccgctc tcccggagct cgtccagggc gatgtggtag ggcatgatga tggaggtctt
     8101 ggcgtcgatc ccgaggttgg ggcgtatgcc ttcggcccgg agcgcctcga tctcggacca
     8161 gagaactttg gggttcagga ccacgccggg gccgatgagg agcctcgccc cgaagaggac
     8221 gccgctgggg gtgaggtgga gcttgtaggt ccggtccccc acgatgacgg tgtggccggc
     8281 gttgtccccg ccctggaacc ggacgacgat gtcgtaattc tcagccagga gatcgacgac
     8341 cttccccttc ccttcgtccc cgaactgaga gccggtgagt atggtgaaca tgatctcgac
     8401 cagccgtcgc atggtaaata accgttaccc tgaagccgcc gccccaattc gatctcgtct
     8461 aaaaggaacc gtctgagaga acctggttaa gaggagccgc cggcgaggcg gaggacggct
     8521 cgggcgatca gctcgccgac gtcgatcccc caggcctggt ggagacccca ccccgagggg
     8581 gtcccgttca cctccaggac ccgaaggcct gaggaggtct cgatcaggtc gacgccgcag
     8641 tagaccgccc cgacggcgga ggcggcccgg acccccagat cctcgatctc cggagtgagg
     8701 gggcacctct cgggggcgcc tccccttgcg aggttgctga tccaggaccc cggcggcgcc
     8761 acccggaaga tcgctccggc gacgcggcct ccgacgacga aggccctgat gtccctcggc
     8821 ctttgagccg tcgattcgag gaactcctgg aggtagacga cccccccctc ctccaaaagc
     8881 tccaggaggg gggaggggtc cccgggggcg aggaggcgga gcccccgccc tttgaagccg
     8941 aagaggggct tgtagaccgc cccgccgtgg gccgagacga actccgccgc cgccgagagg
     9001 ctcgtcgtca ccagggtctc gggatggggt atccccgccc gctggagggc gacggaggtg
     9061 gcgaacttgt tcgccgccct cgcgatcgcc gccggggggt tgacgacccg gacccccgac
     9121 tcctccaggg cggatagggc ctcaaaccgg aaggcgacat ccgaggagcc ccgcctcccc
     9181 aggtcccgca cgatgagggc ggataggccc aaaaggtccc tcccctcccc caggagccgg
     9241 aggtcggagc cgacgagagc cgccagctcc gagaagtcga gatactcggc ctttgcccct
     9301 tgcctagata gccctgaacc gatcgcctcc gccgtccagt ccccccgatc ggtgaccaca
     9361 actccaacgc gcatgagggg ttggagtcgg ccgggatata tcgggctatc ggccgaccgt
     9421 cggcagcccc agcttcgccc aactccggat cccgccgacg aggttgaaga cctccccgaa
     9481 gccgaggcgg gccatcgcct ccgccgccgc cctcgacctc acccccacca ggcagtatac
     9541 cagataggcc ttcctcctat cgagggccga cacctccgcg aggaaggcgg gggagtggag
     9601 gtcgaggttc acggacccct cgatcctgat cctctcgtgc tcctgggggg tccggacgtc
     9661 caggatcaga agctcgggat cgccctctcg atcccggatg agctggatag cctccagggg
     9721 gtccaggtct tgcaccatcc cttcggacat atatatcgag gggagatggg ttatcggaga
     9781 taaaaagcga ggggctcaca aataggggcg caagcccccc gagaaaaaat gagctgatat
     9841 gaagagcccg aggttcactc ggcctcttcc tcagcctctt cttcgactac ttcctcatca
     9901 acgacctcgg tctccactac ctcttcctca gcttcttcct cagcggcgag gacgaagtag
     9961 tactcctcgg agccctccag ggtcaccatg catccggtct cgtcctcgac gtaggcgccg
    10021 aagacctcga cgacggagcc ctcggtgacg ttctcgtcaa cgaaccccca gggaccgggg
    10081 gtcgcctgga agacggtgac gttgacgacc tggctgcaaa ggacgaactc gtcctcggcg
    10141 gcggtcacat tgacggccca gacggtgggt gcgccgggca tatcggcctc ggtgagggtc
    10201 tcggcgacgc cggtgaaggc caccgtcact acctcagcga cctcatcgac gacctcgtcg
    10261 acggccacat cctcagtcat ctcctcagct agaccagctc ctgcagatag caggaacgct
    10321 actaatacca taaagaggct tttatacatt gcagtattcc tccctgagga ggaggactga
    10381 attaccagta taaacattta ttccatgttt tattcagccg ggttttaaaa tatcggagtt
    10441 ggatcaggcc gtaacggctg ttggggccga atgtaacaac tattatgaag ttagagggca
    10501 tataggcgat acgacatggc tcagaggtgc gaagatctgg gagagcggcg gctcgtcagg
    10561 aggatcgcgg agatcctggg gatctcggag agggacgact gcgccatggt ggaggatggc
    10621 gaaaattacc tcgtctggac gacggacatg cttcatcgga gggccgactt tccggggatc
    10681 gcgacgggtt ggcagatcgg ctggatggcc gccgccgtca acctcagcga cgtcgcggcg
    10741 atgggcgccg cccccacggg cctcctctcc gccgcgggga tcccgccgga gacggagttg
    10801 gccttcgtcg aggagatctt cgccgggatg aaagactgtg cccggcggtt cgggacccgg
    10861 gtcctcggcg gcgatctgga cagctccgac gagctgaccc tggtggggac cgccctcggc
    10921 cgggtcgagc gggaccgggt cctccgaagg cggggcgccc gccccggcga cctcctctgc
    10981 accaccggaa ccctgggggc ggcgggggcg gggctccggg tcgccctgga ggccgaggga
    11041 aaggggatcg gatccgaccc ggggctcatc gccctgacga agaggctttt ggagccgttc
    11101 cccaggctgg aggaggggag ggccctcgcc ctatcggggg cggccacctc catgatggac
    11161 aacagcgacg gcctcgtcct ctccctctac gagctcgccg aggcgagcgg cgtcggcttc
    11221 ctcctccagg aggagaagat ccccatcgac cctctggtgg ccctcatcgc cgaggatcga
    11281 gacgacgccc tcgacctcgc cctcaccgcc gggggggatt tcgagctgat cttcaccctc
    11341 cgccccgaga tgatcggcga ggcggcccgg gcctgcctcc tcgctgtcat cggggaggtc
    11401 accaccgagg gggtccgcct ccagtcggct ggtggcacag aagatctgaa gccccggggg
    11461 tacgagccca tgagaagccc cgaaggctga agaacggctc cggacccggg gcgcgaggac
    11521 gtaacgggcg ttggaagccg atcctccgcc gggccggata gatcaccgcc cccggccgcc
    11581 ctctccgcag aagtaggggc tccggaggag gacggccctc tttatcctcc tcttcgtctc
    11641 ctcgaggtcc gcccccgcca ggtccaccag gttgtcgttt cggaggaggc agggcttaag
    11701 cctcccgtcg gaggtgaccc ggagcctgtt gcagtgggcg cagaactcgg tgttgtccat
    11761 cggccggacc acctccacct ccgcccccga gaggaagtac ttccgccggc ggtggagaac
    11821 tctcgtcacc acgccatccg ccctcgcctc cagggccgcc tcgatcccgg cgatgtcgcc
    11881 ggtggccccc cgccccggca ggttcaagag ctcgatcagc tggaggatga cgccgcggtc
    11941 ccgggcgaaa tctaccatct cccagacctc cccctcgttc tccctcagga cgacgacgtt
    12001 caccttgacg gggaggagcc ccgccccgag ggcggcgtcg atcccctcca gaacccggcg
    12061 gtggtccgct gccgtaccgc cggtgatccc cgcgtagacg gcggggcgga gggagtcgag
    12121 gctgacgttc accctggcga gccccgcctc cgcaagagac cttgccctcg cggcgaggag
    12181 gacgccgttg gtggtgaggg agacctcgac cccctccggg gtcctctcca ggatcgactc
    12241 gaggtcgccc cggaggaggg gctcgccccc cgtcagcttc accctccgga tcccgaggtc
    12301 agacccggcg gagatcaccc tcactatatc gtcggccgag atctcgaggc cccggccgcc
    12361 caccaactcc ccctcgtggt ggcagtagag gcaggcgagg ttgcaccggg gggtgacggc
    12421 tatcctcagc cccgtcactc tcctgccgaa ggggtctata agggtcatca gaggggcatg
    12481 gaggggtatg gtatttagat ttcggatcgg aaggcttatg gccacgtaaa cctttgtgag
    12541 cagagatggt ttacgatctc tggacgacgg ggcttctcct ggcgggatac ctcctggcga
    12601 tgacggccgt cggcgccgcg gcggcgaggc ggtctaagac tgtggagggg ttcttcctcg
    12661 ccgagagggg tcttggaccc gccatcctca ccgccaccct caccgccacc atcctcggcg
    12721 cctcctcgac cctggggatg gcggggctcg gcttccgcga ggggctgacg ggggcctggt
    12781 ggctcctatc ggggacggca gggctcctca tcctatcctt ctttttggcg gagagggtga
    12841 gggccacggg ctgcttcacc ctcccggagc tcttggggac gacctacgac cggcgggtcc
    12901 ggctggccgc ctccgccctc atcctcgtct cctgggtggg ggtgatcgcc gcccagatcg
    12961 tcgcatccgg aaagctcctg ggaatcctct tcggcccccg gcaggaggcc ttcatggtcg
    13021 ccgccgccct cgtcttcgtc ggctacaccg ccctgggagg gcagcggtcg atcgtcaaga
    13081 ccgacgccct ccagcttctc gtcctcctcc tggggcttgc cctcgtatcc tggagggctc
    13141 tgcagctcgc cggccccgac ctcctggcgg gagagtcctt cccgacctcg gaggcgaggg
    13201 ggtggccgga ggtggcgggc ctcgtcctcg tcgtcggctc cgcctacctg gcggggcccg
    13261 acatctactc ccggctcctc tccgcctccg atccggggac ggcgaggagg gcggccctcg
    13321 ccgccgccgt catcctcgtc cccatcgcct tcgtcatcac ctccctcggt atcgccgcaa
    13381 gagccctcta ccccgccatc cttccggagg aggccctccc cgtcctgatg gcggagaccc
    13441 tctcccccct gggggcgggg atagtcgccg ccgccctcct ctccgccctc atctcctcgg
    13501 cggacaccac cctcctgacg gcgacctcga tcctcgccct cgacctcttc ggtgccgcga
    13561 agcccgacgc ctccgccggg gagatcctct gggtctccag ggcgggatgc cttctgatcg
    13621 gaggggtggc cctcctctcc gccctccgcc tcccggagat catatccaac ctcctcgccg
    13681 cctataccgt cttcgccggg gggctgatcg tccccctggt ggcggggttt gagagggaaa
    13741 ggctccggct gacgccatcg ggagccctcg ccggtctcct cggcgggggg accgtcgccc
    13801 tcctcttcgg cgggaggtgg cccctcctgg ggatggcgac gagcgccgtc ctcttatttg
    13861 ccgtcagctg gctcctccct cagtccagga ggctcatcag ggggtagtcg gcctttggat
    13921 cgtactccag cctcgccctc accctcgccc cgtggcggtc tatctccacc tcggcccgga
    13981 gcatctttcc ggcgagctcc ccgtagccga agacgttcct tccgagcatc tggcggtcga
    14041 tctccaccga gacggacctg acgaagggct ggagggatat cgactcctcc atcgccacct
    14101 ccaggctctc cgccgtctcg gcggagacgg gcgaccccac gaactggtgg tacagcgccc
    14161 ccagctttat ccccgcctcg aaggcggccg cttccctctc cgtcggcata cgtctatcac
    14221 caccggtgat atgagatagc cgatcagagc taaaaatacg acccccgccg ataggcgaag
    14281 atctccctgg tgccaggcag cagcagaagc tgcccccacc gccagaaccg ggggcacgaa
    14341 cctcgggtct ctgagcttgg ggtacgggat cgtgctgatc atcaaaagag acatcgagac
    14401 catcagaaag gccgtgagca gggggccgtc cagaaagatg ctggcggcaa cgaaggctcc
    14461 ggcccccgga atgggcatac cctgaaactc gccgttcttc tttcccgtca cgttgaaccg
    14521 ggcgaggcga aggatcccgc aggcaaggta aaacgggccc aagatcgaaa aggcagaccc
    14581 gaaatcggaa ccgaaggccg accaagccag ataggcagga gccgccccga aggatacggc
    14641 gtccgccagg gagtcgatct ggactcccag gggtccgtct cccagcctcc gggcgaggaa
    14701 gccgtccagg ccgtcggcgg ccatggccag gagaatcagc gccgccgacg cggcgagcct
    14761 cccctccgac gcagcgagga tagaggcgta gccgagggag gcgttcaata tggatgccgc
    14821 atcggggagc cggacgagcc ctatgatgcc ggtcatctct ccttcaccgc aaccaccgtc
    14881 tctccggctc gcaccttgtc tccttctccg accctgaggc tgtagctggg agggatggaa
    14941 aactccaccc ggctcccaaa cctgatcatc cctatcctct ctcctctatc gacgaactcg
    15001 ccgggggtga cgtagcagac gatcctcctc acgaggaccc cggtgatctg ggtcaactgg
    15061 accgtcccct caggggtgcc gaagacgatc aggttcctct cattcttcga cgacgaccga
    15121 aagaaggcgg gccggtgacc cccgtccgaa tgctcgatcc tggccaccct cccccccagg
    15181 ggggacctgt tgacgtggac gttgaaaggg ttcatgaata tggccacctt ctcgtcggac
    15241 gcctccacca cccggccgtc ggcgggagag accatcccct cgccctgggg tgaccgttcg
    15301 gggtcccgat gaaagaagac catgaagatc gatagggcta aggccgcccc cgatagcagc
    15361 tcgatcccga ggccaaaaag gagggccgag gccaggaggg gaacgcccac ccaggagccg
    15421 gagcctctgg cgagcctcag gccggctcac ccccgccccg cctgatctcg acggccaggt
    15481 cctcgatcat ctcgaggagc tccgggagga ggaggaagac gacgtaggcg atggcgaaga
    15541 gggcgacggc cgatcccgcc ttggcgatga cgttgtgggc ataatagaag ctatcggccc
    15601 caaaccaacc ccatccgtag gcaacgacca cgaagacgtt ccggaatagg ttgaggaggt
    15661 atatcaccgg gacggaggcg acgagggccg ccgccctcct cctcaggggg gcctggaccg
    15721 agaggacgac cccggcgaag agggccatgc tctcgatggc ggtgcaggcg aggacgatct
    15781 ccaccgacct gtcggcgagg actatgacgc tgccgccgcc ggaggccgcc gggatgccga
    15841 gggctcccaa aaggaggagg gtgaggtccg ccgtctccga tatcagccat tccttcaggg
    15901 gctgaacctc ggcgaagggg aagtaggcga acccccctat cgccaccgcc gaggcagccc
    15961 aggccgagga ggcggaggcc gatcccctgg ccaggacgat ccagccgagg tagaggcaga
    16021 agagggctac cgccagggtg aggaggacgt tgaaggggtc tccgatctcc aggtagtggg
    16081 cgggctgctt cagccagtgg acgccgaaga ggacccagcc gagccccgcc aggatccgac
    16141 ggccggagag ggccgaggcg gcgaggaggg cgagccccat ccagaggacg ttcccgatca
    16201 gccagccgat gatcatcggg gatagaaaat ccacaggttt atttagcatt atctaccaac
    16261 cgggaaatct gatacgcccc ggccttttga tctgccgtcg gcgcggagtg ggagtggtta
    16321 gacttatgga cggatatgga tgggggccga tcctggcggt cggccttttg ctggccctgg
    16381 cagcggcggc ttcggccgga tccggggtcc ctcaggacct cgatccgccg atcagcgaag
    16441 ccctgggcct cctcctcgga gacgagctgc cgaatgggac ggagaatatt ccggtgatag
    16501 tggtcttcga ggagggctcg gataaggtgg tgcccgacct cgaggtgaag cactcctacc
    16561 ggacgataaa cggggtctcc ggaagggcga acgcctcgat cctccagagg ctggcggaag
    16621 atccccaggt tctgggggtc tatcccgatg ggacggtggc ggcggcccag ccggagaacg
    16681 cgacccaaga tctgaggaac ttctgcgcca ccggcagggt gaacgccaag cccctctggg
    16741 accgcgggat caacgggacc ggggtggtgg tggcggtcct cgactcgggg atcgacaaga
    16801 accacccgga cctggcgggg aaggtggtgg gagaggtgaa cttcgtcgac tccgagagga
    16861 cgaccgacga cctcctcggc cacgggacga cggtggcggg gatcatcgcg gggtcgggga
    16921 ccgcctccgg cggcgagtac gtaggggtgg ccccgggggc gagcctcctg aacgttcggg
    16981 tgatcgacag caagggctcc ggccagatct ccgacatcat cgccgggatc gactgggcca
    17041 tagataacga cgccgacatc ctcaccatga gcctgggcgg cctcaacctc ggcgagagca
    17101 acccgccgat atccatggcc gccgacaacg ccatggacgc cgggaccgtc gtctgcgtcg
    17161 ccgccgggaa cctcgacgag aggctcctcc tcctgatgct cgtcgcctcc tcggtctccc
    17221 tcgggggggt cgagtccccg ggggatggcg tcaaggtgat caccgtgggg gcgacggact
    17281 gcgacgaccg gatagccacc ttcagcggct ccggccccct ccgggacggg aggtacaagc
    17341 cgagcctggt agccccgggg gtgagcgtcg tatcgacggt ccccctgaag ctggagatcg
    17401 aggggaagat cgacaactat tacgcccggg cctcggggac gagcctctcg acccccgtgg
    17461 tggcgggggt cgccgccctc ctcctccagg ctgacccctc cctcaccccc gccggggtga
    17521 aggctgccct gaccaggggc gcaaagaagc tctcgaacag ccaggacgag gagtacgagg
    17581 cctactacca gggggcgggg ctcgtggacg ccgagaggtc cttcgagctt ttaggggtcg
    17641 accggctctg caacgccctc cctgaccgct ggtggggggg gaggtgggtc tacggcgact
    17701 tctgggtcgt cggggacgac accgtctacg ggagcctcga caccggagcc gacaggttcc
    17761 agaagaagct ctacgccctc gcccccgggg acgtcgactg gagctccaag ttcctcttct
    17821 tcaccgacaa acccctcgcc aacgtcacca ccgtcgcctc gggggaggtg gcgagatgga
    17881 cgatggtcca gcccctcccg aggacgatcc ccgccaacgg ccagcggatc ttcgggatcg
    17941 ggatgagcgt cccggagggg gcggctccgg ggctctacga agggaggata gagatatccg
    18001 agaacgggac cgggatcctc tccatcccca tatccgtgga ggtggcggcg ccggtggaga
    18061 tcctccgggg ccagggatcg gcagcgggct ccctcgtccg gggcgagtgg gactactact
    18121 acgtcgaggt ccccaccagc accgacgagc tgaagtgctc cctccggtgg gagggggagg
    18181 cggacctgga cctcttcctc ctctccccca ccagcgagta cgtccctgcg gagggggggg
    18241 acggcgtgga gtcggtcctg gtggagaccc cccccaccgg ccggtggctc ctcgcagccc
    18301 acccgaggaa cataacggga gagaccgatt acgtcctgga ggtggagagg accctcctca
    18361 ccagctcgcc gaggaggtgg tcggcaggct ccgccatccc aggggagacg aaggaggcga
    18421 ccttcagcct ccggaacggg gggccccccc tcgccaacgt cacctacgtc gggatgatgg
    18481 agaaggtgga gaaggtggcc tggaacgact cgatcgggga caaggtgata tggaccgtcc
    18541 ccatcgaggt cccggccaac gtcacccggc tgggggtgag gctcacctgg gacgaccccg
    18601 atagcgacct cgccctcctc ctcttcaacc agcgaggcag gcccgtcgac gtctcctacg
    18661 gcggagaggt ggtggaggtg acgggggcga gccaccccgg ccccgggata tggagggcga
    18721 tagtctacgg ctatcacgtc accaccaagg cgaggcagac cttcgacctg gaggcgacct
    18781 tctacctcca ggacacctgg tcctgggtct cggccgaggg ccccgaccgc ctcgactccg
    18841 ggacggaggg gagcttcaac gccaccatcg ccgtcccccc cgacgccccc agcgccggcc
    18901 tggaggggta catccagatc cggtccgacg aggaccgctt caccatcccc gtcagcatca
    18961 ccgtggcggg ggcggcgctg acggggtcga gcaggactga gctcttcgac gaggacggcg
    19021 acggcctcta cgagaggctc tgcgtcgagg tgggggtgga cgtcacctcc cccggcgagt
    19081 acagggtaga ggggagcctc ctggacggaa acggctcttc catcggctgg ttcgggagca
    19141 ccgaggagct cgaggcctcc gggtcgatcg agatctgcgt cagcgggacc gatatctgga
    19201 gccggggggg gtgcggaccc ctcagcctcg agaacctctt cctctacaac ggggagggcg
    19261 agatcatcga ccggtaccag gggaacatca ccatcttcca gtgcccccag gacttccagc
    19321 ccctatcggc gaggtttacg gggatcttcg tcgacgagac gaaggaggca gcgccggagg
    19381 cgatcgtcat cggggtggag gtcgccgtcc agacctcggg gagctacgag atcgcgggga
    19441 ggctcgtcga cgacgagggg tttgagatcg acaggacgga ggtgctggtg aacctggagg
    19501 cgggggtctc aatcgtcccc ctcgccttca accccatcag gttcatcgtg aagaacgccc
    19561 cctcgaggct ccacctcaag gacctcaccc tccgccagga tggcgaggag atcggccttg
    19621 tggagaaggc cttctcgacg gaggcgcacc ggcccgaggc cttcgccacg gcgagggaga
    19681 cctacgtcat ctcccccccg gcggcggcct gaggggtcgg gaccgtccgg acccagcctc
    19741 gaactcctcc ctcatgggag gggggacggg ccgggggagg gacttttttt ggatttttat
    19801 tatatcatgg ataatcctta taaaagagga ggctctgaat atagggatga gacgccgccc
    19861 cgaagaggat cagaaggaaa aggtggcgga gggatggaga gggatgaggc atggagagga
    19921 tggcgggagg aacgatattt ggataacgtg aagttgaggc tgaaggcgga ccccctttac
    19981 accaatttta agcatgacgt cacccccgag gggctggccc tcgccttcag gtgggtcccc
    20041 gaggcgacgg ggctgagcga agtggagagg gagctgttgg tgctgaaggc ccgggaggac
    20101 ctggaggcga tctggccgga ggcgcgggac gagagggagg cgatggccct cttccaggag
    20161 aggaccagga cgatccagga ccccgagaag ctgaaggtct acctctgcga gttcctccgg
    20221 gggcttgcga tcctcgcctc cgcctgggtg gaggggcggg agagggcggg gatgaacgcc
    20281 agcgcccaga ggatgtacgg ctgaggcgag gcgtctcgat cgacagacgc cccccccccg
    20341 ctggctttgg aggcgatccc cacccatcat cccctgcctc acctctggcg ccgccctccc
    20401 cctctccggg atcgtgtggg gccccgacgg gagcccgcca tccctaaccc gaatttgagg
    20461 cgtccgccag gatcccaatc cggagggcga gccaatgccc ctcattttag gactcttttt
    20521 aaggcaaaga acaaatatca taattatcct atcttctgaa tatcatggat gggagagcgc
    20581 gaccctcgaa gatcaagcat ccaccccaga taaagaggct gagcctccag ctccagtctc
    20641 tcatcaaggg gaggatgtgg ctgaaggtcc tcctggggat ggtcctgggg acggccctcg
    20701 gcatctccat cggccccgcg gcgggagtcg tagacccggg ggttaccaca gccatcagcg
    20761 agtggctatc cctcccttga tacatattcc tcggcctttt gcagatagat cgtcgtcccc
    20821 ctcgtcttct cctccatcat cctgggcatc gccgcaaatc aggagatgga gatggtaaaa
    20881 aggctggggg tcctcgcagg cctctacttc gtggcgacga ccgccgtagc catcgccatg
    20941 gggataggga tcgccctcct gatcaatccg gggcagtaca tccaggggga ggaggtgatc
    21001 ctcctggcgg ccgccccctg gaggtcccag gaggtcgcgg atctgccgga gatcgccgag
    21061 atcccgggga tcatcggcgg cctcttcccc accaaccccc tcgacgccat ggccagcggc
    21121 cagatgctcc aggttgtcct cttctccgtc atcgtcggga tcgcggtcct caccctctcc
    21181 acccggagct ccgccccctt catggacatc ctcgggtccc tccaggagat ctgcatgacc
    21241 gtcgttaggt ggagcatgct cctcgccccc ctggcggtct tcggcctgat ggcgaagttc
    21301 accgcccagc tggggctgaa gaccctcctg gggatggggg tctacgtggc ggccgtcctc
    21361 ctcggcctct tcctcctcct catcctatat ctggtgataa ttctggccta cctccggagg
    21421 gaccccctcc ggttcctcgc cggctccaga gacgccctcc tcatcgcctt ctccacctcc
    21481 agctccgccg ccaccatgcc cgtctccgtc aaggtcgccg aggagaagct ggggatcagg
    21541 ccggcggtcg gccagttcat catacccctg ggggcgacga tcaacatgaa cgggaccgcc
    21601 ctctatcagg gggtggcgac ggtattcctc gcccaggtct tcggggtgga gctgacgatg
    21661 gcccagctcc tcctggtgat gatcatcgcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21721 nnnngggtgg gggttccggc ggagggggtg gccctcatca tcggggtaga ccggatcctg
    21781 gacatgagca ggacctcggt gaacgtcgcc ggcgacctcg tcgcctcctc cgtcatggac
    21841 aggctagccc gggggaggct ggacaccctc ctggatatcg gggatgggga tgagggagga
    21901 tgaggcggcg ggggagcttt cccccctcgc cgcggccgga cccgaggatc ccggaactca
    21961 tcaaagacga ggcgggggcg gctgcccagc ccttcaccgg aggtagtagg cgttcaggac
    22021 gtactcctgc atcatcctct gggtattgaa gaaggagccg ttgatggcca cggagtggat
    22081 catcacgtcc gtccatccca atctatcctg gtagaatagg gggattatca ccccctccag
    22141 cttctcgtag agggaggcgg cgtccctgga attgtcccgc ccttccccct tcccccggac
    22201 ggggtcgccg atggaccagc cggtgagccc ctcgatatgc ccctcgaccc accaaccgtc
    22261 gaggacggag aagctgggga cgccgttcaa ggccgccttc atcccgctcg tccccgaggc
    22321 ctccatcggc ggctcggggg tgttaagcca gaggtcgacg ccggaggtga tcatcccccc
    22381 cagggtcatg ttgtagtctt cgaggtaggc gatcttgata tcgtcccgga gggcatcctt
    22441 cgcctggaag atccgcctta tcagctcttt gccgccctgg tcccgggggt gggcctttcc
    22501 ggcgtagacg agctggatct cccccgcccc ccgggagatc ctcgccagcc ggtcggaatc
    22561 ccggaagatg aggtcccccc tcttgtacgg ggtcgcccgc cgggcgaagc cgatggtgag
    22621 gatctcctcc tccatctcca cgccggcctc ctcccggacg tatcccaata gcctcctctt
    22681 cgcctcctta tgggcggacc agacctcctc gcgagggatg gagagggcgt agcggaggct
    22741 gaagttgtcc cgccgccaca tggggacgtg gcggtcgaag agctcggcga aggggggaga
    22801 gacccacctt ccgacgtgaa ccccgttggt gatggcgtcg atgcagtatc cagcgaagag
    22861 gagcctggag atctccccgt gctttttggc gacgccgttg atgtaccggc tcatcttcag
    22921 ccccaggtag gtcatgttga ggagattccc ctcgtagagg accccttcga ggccgaagaa
    22981 gtcgtcccta tctccgatga cctttcgggc gaggtcgagg gggaacttgt cgtggcccgc
    23041 ggggacgggg gtgtgggtgg tgaagacgca ccggtcctgg acggccctct tgtcctcctc
    23101 ggtcacctct cgcctccccg ccgccctcgc cgcctcgtcg aggatctcca gggtcaggag
    23161 ggctgcgtga ccctcgttca tgtggaagct cttgacgtcg ccgtagccga gctcccggag
    23221 gatcctgacg ccgccgatcc ccaggatcac ctcctgggag aggcggtagt ggtcgtcccc
    23281 cccgtagagg tgatgggtga gggtccgatc ccagtcggag ttctcagaaa ggtcggagtc
    23341 gatgaagtag acagggacgg cgaagccgtc gatccccgtc acctccctct tccaggccct
    23401 gaggtggaca gtcctcccct ccacctctac ggcggtcctc gcctccatct cttcgaggtg
    23461 ctcctccaca ggccaggccg ccgccacctc ccgctgccac ccgtcgtccc cgatcacctg
    23521 gcggaagtgc cccttccggt agaggagggt gactgcggcc ataggtacct tgaagtctgc
    23581 ggcggagcgg atggtgtccc cggccaggac ccccaggccg ccgctgtagg tgtggatccc
    23641 ctcctcgagg cctatctcca tggagaagta ggccaccgac aaattatctc catcatctcg
    23701 tcccatctcc aaccacctga tatcgccggt gaaggactcc cgacagccgc cctttcggcc
    23761 gtgatgccga tggctgatca gatcaaacga agcccctcgt ctcgccttcc accattatta
    23821 attagatcaa ttgagataaa aggcgaacgg ttagggggag aggaggcaac tccaggccgt
    23881 ttgtcgaccc ctcaaggacc gtcagttttc ggatttctga cgatcccccg gggatgaccc
    23941 gccttccctc tccctcggcc ggcgccccgg tgctccggcg gagacgcctc ggggattttc
    24001 agtcgtgatc cgctcggagc ttcctgtaga gccgctctgc gtcctccttt cggcagagct
    24061 ccgagccggg ggtcatgacg gcggcggccc cggcggcgat cccgaagagg gccgcgtccc
    24121 ggggcgccat cccccgggcg aggctgaggg tgatccccgc caccatgctg tcgcccgccc
    24181 ccaccttgct gacgatcctg accgtcggcg gcctcatgtg ggcgaccgcc tcccggctga
    24241 cgaatagagc tcctgccgag cccagggaga tgacgaggag ctcgcagccg ccgccggcca
    24301 ccaccctccg ggccgccgcc tcgatctcgg cctcctccct caggtccccg tccaccagct
    24361 cccggaactc ccggaggttc ggctttatca ggtagacccc ctcctggagg gccctcttca
    24421 ggggctcgcc ggaggtgtcg acgatcacct tcgcccccgc ctccttcccg gcccgggcga
    24481 cccgggcata gaagtcggcc gggacccccg ggggaaggct cccgctggcg acgaggaagc
    24541 cggagtccgg ggcggaggcg gcgacctcct ccagggcgag ctgcacctcc tcctccttca
    24601 gcaccggccc cggcatcccg aaccggtact gctgccccgt cgactcctcc agaacggcga
    24661 agctctcccg ggtcatcccc gccaggggga gccgccggct gaggagcccc tcccccgcca
    24721 ggagctcctg gaggaggtcg ccgttgagcc ccccggcggc gtagaggagg gtcgactccc
    24781 ccccgagctt cctgaccgcc cgggagacgt tcaccccgcc cccgcccggc tcgatggtgg
    24841 gggcggcgca gcggagcttc ctcccgggga cgacgaactc gacgctggag ctcttgtcta
    24901 tcgtcgggtt gatggctaaa gagacgattg ctttcatgat gatccgaagg atcaatctcg
    24961 cccgcgttat ctaagggtgg tggtccccgg gggcgatgcc aaagattaat acggaagtcg
    25021 gcggagaggt attgggatag accgaaccga gcaaaggagg tgagagagac gagaatgaag
    25081 gggtttatga cgcttcttgc ggccctgtcc gtcctgacga tggcggccgc cgcagggggc
    25141 atgtatcagg gggtctccgg ggggccgtcg atgagctacg ccggggtatc ggggtcgatc
    25201 gacggcatct tcgccagcta cccgaaggcg ccggccgcct tccccgggcc gctcttcatg
    25261 accggcgagg agttctgcca gtttgtcgcc gcccacctcg cagacccgcc caccgcctgg
    25321 tggtggtgac gggtcctgag cggggcgcaa gggcggaccg aaaagcccgg ctcccggggc
    25381 ggggatggac cgctctagcc tttttttgcc aggggaagct tttgagatgc gcaaacatcc
    25441 tgtacgcgaa aggcgctcgg gcaccttcga ggagatgtag gccacgacca gatagtttat
    25501 gcattcgtgg aggggcgtta tacttctgca aaaagaatca tataattatg tcctttttct
    25561 tttgcgcatt ttggacaata tgcataatga acatagtaat ctttagcttt tttattctcc
    25621 tttgccaaat atatttccat ttcttttata aatttaggga tggcattata tggtccatca
    25681 aaaactccag caacaaaagt cccagaaata gttgtattat ttgctccctc aaccatttta
    25741 ctgactgtgt aatagatttc agacttaaat gcggaaggat ctcgaaacaa aacgagagca
    25801 tctgtcttat ctggaatatc tgcttgagct ttttccgcta aattccacat cctcataatc
    25861 ttctttccaa tcatgggcgg aaatgggata tgataaattg taggaataga ttcttttata
    25921 aacaatttat ttttccaatt gaacgtcttt ttatcccatt ttattggatc aaactctggg
    25981 caacattcat cttcattcat attgttcacc tttagcgcaa tttgatagat tagtatataa
    26041 atattacgaa taacgcttct ccatctttct aagattaagt aatccaaagg ttgaagatga
    26101 catcgcgata aagagaagta cgacgaagtc ggatttgggt gaagcgaggg tccacccaag
    26161 agtagcctct catacaaact atatgcgcat ttgggcggga ttatttatct gaaatcaatc
    26221 ttatagacca tcataaaccg ctatagctaa accgtactta agaattcttc ttccgcccgg
    26281 ctccggtcgg agggaaagag gatcggcgac ttcgacgagg tgatcgcatc gatcgccctc
    26341 gtccgcgacc gggagatggt gacgagagac gagcactttt gggaggtggc ggggctggtg
    26401 gtggtggggt ggtgaggtag aggggccttt ttgaggggtc cgttcgcagg gggtgcggac
    26461 tgaggcgcgg ggggattacc cgctgatgtg gtcgaggata tgcttctgca cggccgtgct
    26521 gatggtcgat tgctcgacga ccatgacgag atgacctccg cgatcgaagc tgaccagagt
    26581 ggcccccgga atggtggcag cagcgaactg ggcattgtgg tagagctgga gggtgtcgtc
    26641 ggtggcatgg acgatcaggg tgggggctgt gatcgaggcg atcctctcat tgggcatcgc
    26701 cgccttgttg tcgaaagcgg cgccggcggc ccggggcgag gccgggttca tataatcgat
    26761 gatctgttca gccacctccc gttgctgggg cgatagtccc gcaatgaccg tgtcgtcgac
    26821 gcccatcagc tccatgaact gcttcttgaa gaggttggtt atggtccagt agagcggatc
    26881 ataccggtag atggtcacca gcatctcgcc ttgccggttg gcctgggcct ggtcggtcgc
    26941 gtttgaagag gcgaccccgg cggagatgag ggccagggag gagacccgct cagggtgcaa
    27001 tactgccagg agcagcgccg aagggccgcc atgggagatg gcgacgaccg cagcttcttc
    27061 gacgcccaga tggtcgagga gataggcgta ggcatgggcc tggtcgtcaa atgtagcccc
    27121 ctcgtggaag gtcgagcgga gatagccgaa acgcgaaggc gcgatccagt ggaactgatc
    27181 accgaggacc gcctgcacca ccagttcgcc ctgatcgtag ccgccgccgc tcccgtggat
    27241 caccaggaca ggaggccctg atcccccctc ggtgtattcg atatccccat agggcgaagg
    27301 tatgaccgtg ctcttcccct ggatccgctc atagtctcgg ttaatatccg agaggtatgc
    27361 tgcggaaacc agaaccaccg ctaagatcaa ccctatcgca atccagcgca gaatctttcc
    27421 aagcatcttc tcctcctttt tgggaccgcc caccagaatg gatctcctga ttatcacgga
    27481 tcaacgcagg cctcgcaggc tttgggtttc gatccctgtc tttggtggac tcgcacctgg
    27541 agaaatttgt ttcggtcgtg gggaggcata acggattatg tttcaatccc tgtctttggt
    27601 gaactcgcgc cgatccgcgg ggcggggatg gaccgctctc gccttttttt tcccagggga
    27661 cgcccagggg gcggaggatc ggcgggcggc cgaggtgctg gaattgtatc ttgccggtat
    27721 cgcctgcgaa ggtcggcggg gcataaatac acgtacatac atagatcgat ccatggcgac
    27781 taagacgatc agcataaccg aggaggccta cgagaggctg aaggacctca agggctccga
    27841 aacgatgagc ttctccgagg tgatcctcag ctactacccg aggcggagga agctctccga
    27901 gaccctggcg gagatcgccc gcccccaggc cgaagacctc gccgaccaga tcgagaggac
    27961 gtcggaggag ctccggtcgg cgaggatgag ggcggtcgag ctctgatgcc ggtcctcgat
    28021 acctccttca tcgtagacct cctacggggg cgaaaggagg ccggcctcct cctcgcggag
    28081 ctggaaggcc ggggcgccat cctcgccacg acgccctaac cgccatggag ctgtatcggg
    28141 gagccttcct ttcggcggat cctgaaaaga acctggcggc ggcgaagaag atcctcgact
    28201 ccctcatcgt cctacccctg gacgacgatg cagcaaccgt cttcggcgcc ctctcgcctc
    28261 ggctccggtc ggagggaaag aggatcggcg acttcgacga ggtgatcgcc gcaatcgccc
    28321 tcgtccgcga ccgggagatg gtgacgaggg acgagcactt tcgggaggtt gcggggataa
    28381 aggtggtggg gtggtgagga ggagagggct gcttcaatgg ggccacgcct tttcagacgt
    28441 ggaaatttca ttccccaggg catactcggg acggttccgg cgaggcttca atggggccac
    28501 gccttttcag acgtggaaat tctccctacc cttgatgctt tcgaaagcta tttgagtggc
    28561 ttcaatgggg ccacgccttt tcagacgtgg aaataggctt tggtcgtgtc tgcggggctt
    28621 gcactggccg tgcttcaatg gggccacgcc ttttcagacg tggaaattac ctcccatgcg
    28681 ctgcctaccc tctttcaaag cctgcttcaa tggggccacg ccttttcaga cgtggaaatt
    28741 atatttgtgt tgggatgtgc gcagtttgtt tttgcttagc ttcaatgggg ccacgccttt
    28801 tcagacgtgg aaattgatct atcccgacat gatcactgtc cgggccgatc tgcttcaatg
    28861 gggccacgcc ttttcagacg tggaaatata gaaggtcaag acggctttcg tccccggtct
    28921 ctgggcttca atggggccac gccttttcag acgtggaaat tccgggcggg tagagttgtc
    28981 ggatctgacg attggttgct tcaatggggc cacgcctttt cagacgtgga aatatcgcgc
    29041 gtgtgaagct gaatgaaaac gccagcggct ggcttcaatg gggccacgcc ttttcagacg
    29101 tggaaatcct ggtttgcagg cacttcgacg cccaggacga tcgcgcttca atggggccac
    29161 gccttttcag acgtggaaat cgggtttgcg gcctcctaac gccgacccgc cccggccgct
    29221 tcaatggggc cacgcctttt cagacgtgga aattcgatat atttggcgta gcagtatcca
    29281 tccgcccagc ttcaatgggg ccacgccttt tcagacgtgg aaattctccc gatctctggg
    29341 ggcgagaatc cggagactga cgcttcaatg gggccacgcc ttttcagacg tggaaatcgg
    29401 gccagacgtc ctcggttatg cttccctgga cgacgcttca atggggccac gccttttcag
    29461 acgtggaaat tcgggcatgg gatgagggtg agactctcac gatcgagctt caatggggcc
    29521 acgccttttc agacgtggaa atcgagatcg aaattttcat agaacgcttt ggaataatgc
    29581 ttcaatgggg ccacgccttt tcagacgtgg aaatccaacg tctatctcgg gggcaacgtc
    29641 gctgaggtcc agcttcaatg gggccacgcc ttttcagacg tggaaattcg agaaccttat
    29701 agtaggttcc gcatttggga catagcttca atggggccac gccttttcag acgtggaaat
    29761 gggagccgaa ctcaaggtcg cacctccagt ggacgacgct tcaatggggc cacgcctttt
    29821 cagacgtgga aattagcttg tttaaaaaaa tacgttaaat gcgtaagcat acagcttcaa
    29881 tggggccacg ccttttcaga cgtggaaata agagccagcg ggcttggctg aacacccggt
    29941 tggtttgggc ttcaatgggg ccacgccttt tcagacgtgg aaatgtactg gtcccgacgg
    30001 gtccctcccg caccgaagac catgcttcaa tggggccacg ccttttcaga cgtggaaatg
    30061 tctcggagca atgggaggcc acgttcgcga atcccagctt caatggggcc acgccttttc
    30121 agacgtggaa ataacactct ggtagcggtt gaagacgatc ttactctgct tcaatggggc
    30181 cacgcctttt cagacgtgga aataaaagtt ttacctctgt tacacctgca cttactgttg
    30241 cgcttcaatg gggccacgcc ttttcagacg tggaaattcg gcctcctcct cgatctcacc
    30301 ttcccagagc cccggcttca atggggccac gccttttcag acgtggaaat ctcacagagc
    30361 tccaggagat cgagccagac acctatgctt caatggggcc acgccttttc agacgtggaa
    30421 atcgatcgaa caactacggc cggagctggt ccgagatctg gcttcaatgg ggccacgcct
    30481 tttcagacgt ggaaatgatg ttcggcataa gacccttgat cctcttttcc agcttcaatg
    30541 gggccacgcc ttttcagacg tggaaatcgc agtgaagagg gtcgtggtga cgttgtaggt
    30601 tgcctgcttc aatggggcca cgccttttca gacgtggaaa ttcgtgaaac tcgtcttaac
    30661 tccttttggc gcgaagaggc ttcaatgggg ccacgccttt tcagacgtgg aaatggttcc
    30721 cggacgatga ggccggatac ctggccgccg cgcttcaatg gggccacgcc ttttcagacg
    30781 tggaaattgt cagcccaaac ttcggggcag cggttgtaga gccgcttcaa tggggccacg
    30841 ccttttcaga cgtggaaatt ccaatgccgt tcttcagctc ggatgccacg atatggcttc
    30901 aatggggcca cgccttttca gacgtggaaa tctgctgggg cctttcgccg tagatcccgc
    30961 accaagtgct tcaatggggc cacgcctttt cagacgtgga aatcttacgt tattctgtgc
    31021 tccattcaga atgatgattt agcttcaatg gggccacgcc ttttcagacg tggaaatcgg
    31081 gctcggtcat cgttgccgga acccaggccg gggccccatt gaagctaaat catcattctg
    31141 aatggagcac agaataacgt aagatttcca cgtctgaaaa ggcg
//
LOCUS       DASM01000126           11230 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_39979, whole genome shotgun sequence.
ACCESSION   DASM01000126 DASM01000000
VERSION     DASM01000126.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_39979"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2045 a         3472 c         3663 g         2050 t
ORIGIN      
        1 gggttccggt gagcgcaaag ctggggggcc tcgacgccct ccttgccatc tcccagatgc
       61 cgccaggggt ccccgtcgcc tgcgtcggga tcgacgccgg ggagaacgga gccctcctgg
      121 cggtgaggat attgggggcg aaggagcggc cccagggatg agggtagatc ctataggtgt
      181 cataatatgt acgagaagat cctgatagcc accgacggct ctgccgcctc gatgaattcc
      241 agcaagtccg ggatcaagct cgcgaagtcc ctggggtccg aagtcctcgt cctctacgtc
      301 ataaacgaga tggcgatatc cagggtcatc gcctccatga tcggcagggg tttccgtcaa
      361 gaggatctga ggctggccct aaaggcctcc gccgagaagg tcgtctcgga ggtcaccaag
      421 atgggcgagg ccgcaggggt ccaggtggag cccatcataa aggaggggga ccccgcctcc
      481 aggatcctgg agacggcgga gatcgagggg gtcgacctga tcctgatggg aagccacggc
      541 gaagggggga tatcttcgaa gctgatcggg agcgtcgccg agaaggtcct gaactggtcc
      601 acagcgccgg tgatgatagt gagatgaccg gggcttgcag cggcgtattt atccccttta
      661 taaggcttct tgatgccggt atcggaaaga tacctattta tatcaaaggc gtaatggata
      721 aactcaaaag gtgcggaagg ttgtagggag atttgagagg tgtgataaga tgtatgatcg
      781 gattttagtt gctacagacg gctccgccaa gtccaaggtc gcagtagagc acgccgtaga
      841 cctggccaag gccacgggcg cgaagctgct cggagtttac atagtgaatg aggtcgtggt
      901 ggccagcgcc gtcaggcagc tgggcgcgga caagaaggag atcgagtcga agctccaggc
      961 cagcggagag aaggccgtgg ctgatatcaa gaagatggcc gacgaggccg gtgtggccgt
     1021 cgagacgatg gtcaggtccg gggctcctgc caacgccatc gtcgacctcg ccaggaccga
     1081 ggaggtcgat ctgatcgtca tgggaagcca tggtgagagc ggcgtcacca agctcctcat
     1141 cggaagcgtc gtccagaaag ttctgtattg ggcatcctgc cccgtcctcg tagtgagatg
     1201 atcgaagatg gcagtagaga tgatatcctc agggatcgct tttccagctt tcgcggtggt
     1261 attagtcgtc ggaacgctcc tggcctatac ggtcataagc ctcataggcg gcagctagct
     1321 ctccagaagg gcgccctcct cgcggagggc gctatcatca tccctctttt tggcggtcca
     1381 tcccccaggg catgaggctc ttgggagata cgatcttgga aaaataggtg agcttcggcc
     1441 ttctgccgag atcggtggcc gtatcaatcg ggcttgaagt acctctccgc cgtctccagg
     1501 gcccgggcga tcagctcggc gcaggcatcg acgtccttca ggctcaggac ctcgacgggt
     1561 gagtggatgt aacgggtggc tacgctgatc ccccccgtat gaataccctc ccgggtgagc
     1621 tggatcgccg tggcgtcggt ggtgccgccg ccggagacct ccatctggac ggggatctcg
     1681 aactccttcg ccgtctcggc gagccaggat acgacctttg aggtggtgat gatcccccgc
     1741 cccgaggcgt cggcgatgat caccaccggc ccctttccta tctcgagggg cgagtccttc
     1801 ttgtcgatcc ccggatggtc tcctgggatg gtgacgtctg tagcgagagc gacgtcggga
     1861 gagaggccga aggcggaggt cctcgccccc ttcagcccca cctcctcctg aaccgtcccc
     1921 acggcgaaga tcgtcgactc cgtcctcgtc ctcttgaggg cctcgatcat catgaccaca
     1981 ccggcgcggt tgtcgaaggc cttccccgtc accctgtccc cggcgagcct ggcgaaggtc
     2041 cgatcgatgc tcatgggggt tccgggcttg atcccaagcc ccaaggcctc ctcctggctt
     2101 tcgcagccgg cgtcgacgaa catatccttc acctcgatcg gcttcttccg gtcttcgggg
     2161 gtcatgacgt gggggggctt cgacccgatg acgccccata tcggcccgga atcggcgtgg
     2221 atgatcaccc tctggttgag gagggtctgg tcgaaccagc ctccgatggt gacgaaccgg
     2281 atgaacccct tctcgtctac gtacttggtc atgaggccga tctcgtcgat gtgggctgcg
     2341 atcatcaccg acggccgctt cccctttctt gtggccacga ggtttccgag gttgtcgatc
     2401 ctcacatcgt cgacgagggg tcccatctct tcccggagga tctcctggat cgatccctcc
     2461 cgccccgata tcccgtgagc gttgctcagc cgttcaagaa gcctttgaat ctcgtccatc
     2521 tctttcatcc cgtccataat aatcggtaca atcggggctc tgaggtcagc agatcctcgt
     2581 ctccgcgccg gagttttggg agctggtgac gatgacctcg ccccctctct cctccatgac
     2641 ctccctcgcc gccgcctcga tctctcgcac gccggagtcg gcgacggcgt agaccgtcgg
     2701 gccgaaggag ctgagccccg ccccggcggc tcccgcctcc ctcatctcct ccatcagcct
     2761 tcggacgagc gggtcctgga gctccacctc caccctcttg aaccccaggg cctggatcct
     2821 gttcacagcc gccccgaagc tctcgatctc cgccgtcatg accgccggca tcatcccgac
     2881 caagatctcg cggcagagct ctgccacctc ctccttcggg acgggacaga aggccccgaa
     2941 gacgtcggcc tcgtggcggc cgtgggcgcc cttaggcaga ttcgggatcg ccaggataaa
     3001 cctccagtcc ctcggaactt cggccctcat gataaccggc ggtggcgcga cccccgggga
     3061 ggcggaggag gggcgatagt cctccttctc ccttcccggg ccgaaggagt ggccgccgtc
     3121 gacgatgaac cctcctccct cgaaggcggc gacccctatc ccgctcgtcc cgcccctccc
     3181 gacggccttc gcgatctccc gggccggagg tgatcggccg tatagctcgc atagggcctt
     3241 ggccacggcc atggcgagct gggtaccccc gccgaggccg acgtgctgct ggtagaagga
     3301 cctgatcctt atatcggcgc cgccgaggcc gaagctctcc atcgccccga gggccgccgc
     3361 cctcgccctg tcggcgccct ctccccggac ggtgagggcc tcgccccgct ccgcctccac
     3421 caggatggag ggctcctcca gggctatccc gacccccccg tcgacgcgcc ccaggtctcc
     3481 ggcgaggtcg gtgagggtga tgtggatccg agagggggtc tggactatca ccctcctcct
     3541 ctcctggaac ttgtggtagg ggaaggtctc ggtgatggtc atcaggggct tcccatgtcg
     3601 gatgatcttg tactccctgg agaggagggg ctctttggag aggacgccga agattcctct
     3661 caaatcggcc gcctccttca gggcccggaa cctcaggagg tcgcgcctcg actcgatccg
     3721 gtgcctcttc aggatcgtac cgatcggcat atccgcccgc atcaggtcgg tcttgaaccg
     3781 gtcctcaagc cgcttgatgg gggtgaggga ggcggcgtgg attagggtct cccccgaggc
     3841 ggagctcttc agcttcacca cccggtagtt cacctcatcg ccggcctcga tctccagctc
     3901 ccgggccgtc gcctcatcgg cggccaccac cctctggacg agggtctcga tctcgacggg
     3961 gcttccggtg accacctcca gaaggcaggt gacggagccg tcggtcccca ggagcatctt
     4021 ctggacgggg ctgagggtcc ccaccgcctt ctcgagcctt gcgatctcct cgtccaccct
     4081 caaagtcggc gatccgtcca caaaaaagga ggtgccggct ggcataaagt ttttcccctc
     4141 cggggacgag ctttcccctc tctgggcttc gtggaggatt ttcattgaag acgtcgagaa
     4201 tttcggggtt ttataagctt tcagcgccgg atcggctgaa gatggtggcg gagaactctg
     4261 gcctctccga ggcggaggtg gaggcgatcg gcagcctggg cctctccctc gatcaggccg
     4321 acaagatggt cgagaacgtg gtgggcctct ttcagatccc cctggggatc gccaccaact
     4381 tcctcatcga cggccgggag gtgctcgtcc ccatggctac ggaggagcct tcggtgatcg
     4441 ccgccgccag caacggggcc aggatggccc tcgccggggg gggcttcgtc accagcagca
     4501 ctgagcccgt gatgcgggcc ctcatccagg tgacgggggt cgatgacccc tggggaacgc
     4561 ggctcgccat cctcgcccgg tccggggaga tcgcggcgag ggcgaacgag aaggacccga
     4621 tgctcgtcag ccatgggggc ggggtcgtcg gggtcgaggc ggaggtcgtc gagacgagga
     4681 aggggccgat ggtggtggtc catctgctcg tcgactgccg ggacgccatg ggcgccaacg
     4741 ccgtcaacac catggccgag gccctcgccc ccttcatcga ggagatcacc ggcgggaggg
     4801 tctacctcag gatcatatcc aacctcgcgg acctccggct cgcccgcgcc cgggccgtct
     4861 tcaaggccga ggagatcggg ggggccgagg tcgtcgacgg catcgtcctc gcctccgccc
     4921 tggcggagag cgacccctat cgggccgcca cccacaacaa ggggatcatg aacggggtga
     4981 cggcggtgac catggcgacc ggtaacgaca ccagggccat cgaggccggt gcccactcct
     5041 acgccgcccg gggcggcagg tacaggtccc tctccgtcta cgagaagaac cgggacggtg
     5101 acctcgtcgg atctatagag ctccccgtcg ccgtggggac cgtcgggggc gcgacgaggg
     5161 tccaccccgt cgccagggca gccgtccggg tcctggggct cagatccgcc gacgacctcg
     5221 gccggatcct ggcctcggtg ggcctcgccc agaacttcgc tgccctccgg gccctcgcca
     5281 ccgaggggat ccagaggggt cacatgtctc tgcacgcccg gaacgtcgcc gtccaggccg
     5341 gcgctaaggg ggatgagatc gaggcggtgg caggtaaaat ggcggcggag aagaggatca
     5401 acgtagatcg ggccaaggag ctgctgagcg ggatgagatg agcccgttga ttggggtctt
     5461 ggggccggag gggaccttct cggagaaggc ggcaaggcgg ctcagccccg gagccgagat
     5521 ccgctacttc cgggacttcg aggaagtgat ctccgctgtt gaggtggtgg atgtggatct
     5581 gggggtcgtc cccctggaga acagcctgga gggctccgtc ggcgcgaccc tcgactccct
     5641 cctccgccac gatgtcgaga tcgtcggaga gatcaacctg agaataaggc actgcctcct
     5701 cggccgcggc ggggcggagg gggtgagggt gatcctctcc cacccccagg ccctcgccca
     5761 gtgcaggggc tacatcaaga ggaggtttcc ggaggcagag ctgaggacta cgggatccac
     5821 ctcccacgcc gcccgcctcg cccaggagtt tccggagatg gcggcgatcg ccgacgccga
     5881 ggctgctgga aggtacggcc ttgcggtgat tgagagggac gtccaggact ccgacgagaa
     5941 cgtcacccgc tttgcggtgg tgggaaggtc ggcgccggcg ccgacgggaa gggacaagac
     6001 ctccctcgcc ctctacctgg agcggacgga gccgggggcc ctctgggagg ttctggggga
     6061 gtttgcgacg agggggatca acctcaccaa gatcgagtcc cggccgagca ggcgggccct
     6121 cggagactac tacttcttca tcgacctgga ggggcacgcc tccgaccccc aggtgaagga
     6181 ggccctggcg cggatcaggg agagggcggc ggtgaccagg gtcctcggct cctaccctcg
     6241 gactgactag ttcgtccctt cgctggatag gcggagcccg ctggcctcac cccaggggcg
     6301 gggatgaccc ccgtttttga gagccgacct ggcccccctc tcgtcggtct atgagccacc
     6361 gccggtcccc ccccgagcct cagacggttt gggagggagg ctttgggacg gatcttccaa
     6421 taggagatcg gggacggttc gatggagaaa attgacttcg gtcgaccgcg cagaccccaa
     6481 attaggcctt cgcgatggcc tatagattta tctaatagaa atgtagcata ctcgctagca
     6541 cgtcgagctt tactacttca ggtcaatttg ggaggcgacg agattgggaa agataaaatt
     6601 agcaattgca ggtctcggca attgcgccag ctctctcatc caggggattg aatattataa
     6661 aaattcagat gacgataact ggatagggct gatgcatcgg tccgtcggtg gctacaggcc
     6721 gggggacata gaggtggtgg ccgccttcga catcgatgag aggaaggtgg gaaaggacgt
     6781 cagcgaggcg atcttcgccc cgcccaactg caccaagacc ttctactcca acgtcccctt
     6841 catgggggtg gaggtgaaga aggggccggt cctggacggc gtcgcccctc acatggcctc
     6901 ctacgacgat agcaagacct tcgtcgtctc ggaccgcgag ccctgcgacg tcgtcaggga
     6961 gctggaggag agcggcgccg aggtcctgat aaactacatg cccgtcggct ccgaggaggc
     7021 cagccggttc tacgcccagg gcgccctgga cgccggggtg gggtacgtca actgcatgcc
     7081 ggtcttcatc gcctccgacc cggcctgggc gaagaagttc gaggaggcgg ggatcccgat
     7141 cgtcggggac gacgtcaaga gccagatagg agccaccatc gtccacagga ccctcgccaa
     7201 gctcttcaac gaccggggct gtaagctcga ccggacctac cagctgaaca ccggaggaaa
     7261 caccgacttt ctgaatatgc tcagccagga ccggctcaag tccaagagga tctccaagac
     7321 cgaggcggtc cagtcggtcc tggacgtccc cctgatggcc gacgatatcc acataggccc
     7381 ctccgactac gtcccctggc agaaggacaa caagatctgc tttctccgga tggaggggag
     7441 gatcttcggg gacgtcccca taaacctgga gctgaggctc tcggtggagg actcccccaa
     7501 cagcggcgga tcgacgatag acgcgatcag ggtctgcaag atggccatcg acaggaagat
     7561 cggcggcccc ctcctggaga tctcggctta taccatgaag catcccccgg tccagtaccc
     7621 ggacttcgtg gcgagggagc ttctggagag gttcctcaag ggagaggcgt tggatctcgc
     7681 ccccgagcct cccctgaggc tgaggctggc caccgcggtc caggataagg gcgattgagc
     7741 tgacataagc ccgatctccc gccgacgagc tccgccaagc ctcccatcca gccccgatcc
     7801 ggctcctcga ccgtcgcccc tcccaagaga aggagctgcc ccctccaaag ggctggaagg
     7861 gggggtccct gcaagcccaa gccccgaccg tcctcatcat cctcaggacc ttatgcaccg
     7921 gtctatcggc ctatgggcat aggtgagaag gaggatcatg agggacgaaa aggccctggc
     7981 ggaagactgg gggggttgta gtccccatcg acggggtctc ccttcggggc ggaaggttgg
     8041 agaaaggcgc aacgggtgat ggatatatgc ggtcttcacc agtggagaac tcggcgtatg
     8101 ggaggcggcc tctccctccg gccgacggat atcccgatcg tctctggtcg ctcttcgagg
     8161 ttgctatccg gggctcttct gtcgacttct ctggaggctt atcgtgatcc gggttctgat
     8221 cgtcgacgac gaccccgcca ttctggagct tgccgccctc ttccttgaga ggtgcgggga
     8281 tatccaggtg gatccgacgg agacggcggc cgaaggcctg gaaaggctgg aggggggcgg
     8341 gaggaattat catgtcgtcg tctccgatta cgccatgcct gggatggacg gtatagcctt
     8401 tctcaaggcg gtccggagcc ggaagctcga gatccccttc atactattta caggaaagag
     8461 ccgagaagag gtggtgatcg aggctctaaa ctccggcgcc gactactacc tccagaagga
     8521 cggaaacccg aaggtcctct tcgccgagct ctcccatcag gtccgacagg ctgccgagag
     8581 cgttagggcg aagagggagg acctcatcca gaggaatctc gccctagggc tctcgggggc
     8641 gacctcccta tcggcggccc tcagcctctg cgtcggggcg gcgatagccg tttcggagac
     8701 ggactgtggg acgatctacc tcttggagag gtcctcgggg agcttcgttc tctcccactt
     8761 ccagggcttc tcccccgagt acgtcgaggc gatatccagg aggcgccgga accccagcac
     8821 ctccctgata tcgagggctc aggagccgat ctacatccgg caccaggatc ggggtcttcc
     8881 cctccagaag gcccgggagc gggaggggct ccgggccatg gccgtcgtcc cgatccgcca
     8941 cgagggggag gtgataggct actacagcgt cggaacccac accttcgacg agatcccccc
     9001 cgaggggagg agcggcctgg agactatcgc cgccatgacc gagaacacca tcgtccggct
     9061 gagggcccag gaggagctcc gaaggaacga ggagcggctc cgggcggtct tcgtgaccgc
     9121 cgagaacgtc tcttttgtca tcaccaacgg cgaagacccc gatccgaggg tcctggagtt
     9181 cagccccggc gccgagaaga tcttcggcta ccgtcgggag gagattctgg gggatacggc
     9241 atcccggctc cacatccccg aggatgttgc cctctttccc gagatgcacc ggcggatgag
     9301 ggtggaacgg cgggggtttt ccgtcaccac taccctggtc cggaagtccg gggacaggtt
     9361 tcctgccatc ttctcgggct atcccctcct cgacggcgag gggaatatgg aggcggtcct
     9421 cggggttagc gtagacatca ccgaagagaa gaggatggaa gagaagctcc tgaagagcga
     9481 ggagatgtac aggaccctgg tggaggacca gccagagctg atctgccgct tcctccccga
     9541 tgggaccctc accttcgtca acgaagccta ctcccggtac ttcggacgga gcagggagga
     9601 tctcgtcgga gagagcttcc tgcccctgat cccggaggag gatcgagaga aggtccgggt
     9661 ccacctagcc tccctctcca gggatcggcc gacggcctcc ttcgtccacc gggttatcct
     9721 acccggcggc gatctgcgtt ggatccagtg gaccgaccgg gccatcatcg gagctggggg
     9781 ggaggtgggg gagtttcagt cctccggaga ggatataacc gaacggaggg ctgctgaaga
     9841 ggccctgaag gagagcgagg agcggcttaa gatggccgtc gagggcggga acctgggggt
     9901 ctgggactgg aacctggaga cgggggaggg gtctttcagc gataaatggg gcgagatatt
     9961 ggggttctct ccggaggagc tgaagaccca ccttgggatc tgggacctcc tcgtccatcc
    10021 cgaagaccgg gtgagagtaa agcaggccct gatggatcac ctggagaggc tggcccccta
    10081 ctgggagacc gaacacagga tgaggtcgaa ggacggtgga tgggtctgga ttttgggccg
    10141 gggcaaggtg gtatcatggg acgagagggg ccgtccggtg aggatgacgg gggtcatcca
    10201 ggagatgagg gcggcaaggg gcggatcccg gggtcatgct ggcaccctag tcgatggaga
    10261 cccggcggga gaggtgccgc cggattagag gctcagagaa cctttttatc cgggtcccgc
    10321 caacggcctc tctatgacca aacaggttca tgcggcccac attcttgtaa agactgagaa
    10381 gaaggcgaag gaggttctcg agaagatcag caagggagag agcttcgccg agatggcccg
    10441 gaagtactcc gactgcccct cttcgaagaa caagggggac ctcggatggt tcgctaaggg
    10501 caagatggtc cccgagttcg agcgggccgc cttcgaggcg gagaagggga gggtcgtcgg
    10561 ccccgtcaag accgacttcg gctaccactt gataaagatt attgataaga agtgacccct
    10621 aacggagttt ttatcatcgg gttggttttt ctggcgatcc ggctttgaga tcgcctcaag
    10681 gcgacctgtc ggactccgga atggcacctc gagctcgatc tggcggagtc atccccctgc
    10741 caaatctata aattccattc cgccataatt gagggagtga tgacggatct gcccctcatt
    10801 ttcggaatga ccggagatca ggcgatgatc ttcggagttc ttcttctcac cctcatcctc
    10861 ttcatctggg gccgctggag gtacgacgtg gtatcccttc tggcgctcct ggcggccacc
    10921 gtcaccggcc tcgtcccctg gaatcaggcc ttcctcggct tcggccaccc cgccgtcatc
    10981 accgtagccg ccgtcctcgt tgcgagccgg ggcctcctga actcgggggt cgtcgatgtc
    11041 atggccgggt ggctctcccg ggcaggagat tcgccgaccc gccaggttgc ctccctcacc
    11101 ggcctcgtca ccatcctatc cgggttcatg aacaatgtcg gcgccatggc tctcctcctc
    11161 cccgtcggga tccggatggc tatccggagc gggaactctc catcgatcct tctgatgccc
    11221 ctcgccttcg
//
LOCUS       DASM01000127           13133 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_92996, whole genome shotgun sequence.
ACCESSION   DASM01000127 DASM01000000
VERSION     DASM01000127.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13133)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13133)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13133
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_92996"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2510 a         3982 c         4305 g         2336 t
ORIGIN      
        1 gaggatgaac ttccccttca acatcgccga aggaggaggc ggagatgttg aaggggaagt
       61 tcatcctcca gatccccatg gtattctcga cgtccaccgt ctgtccgaag agggctgggg
      121 ccagggggga tcgatcgggg ttcgatacat aagacgccca gtacaagtct gcataatccg
      181 agaaccgcga ttctcctgtc cacgtcatca ccgatgtatc tcccatgagg aagacgctct
      241 gggtagtagt atacccatct cccgatgcga tgcccgacag gcacgccagc atgatcataa
      301 ctgcacattt caattctggt caccaccaat tttttcaaaa tcgggatgcg atacaccttg
      361 atcggtacca acctatttat gccttatctg cagtgagatg aggagatgcc agattgcttt
      421 gaagtccttc acaaggatct ggcaggtcgg atcggcaggc ttcggacgcc ccatggcgtc
      481 gtcgagaccc ctgcgctgat gccggtggtg aacccccacc tcaggctgat cgaacctgcc
      541 gatctcgaga ggttgggagc cgagatcctc atcaccaaca gctacatcat ccatcaggac
      601 ccggagctac acaacttagc cgcgagggag ggggtccacc ggctcctggg gtttgagggg
      661 ccgatcatga ccgactcggg ggcctttcag ctctcggtct acggcaatat cgacgtccag
      721 cccctggaga tcctcgattt tcagcgtttg atcgcctccg acatctgcgt ccccctggac
      781 atccccacgc cccccgacgt ccccaggtcg agggccgagg cggagctagc catcaccgag
      841 gagaggctct ctgaggcgcg ggaggtctgg agcgaaggcg gctccctcct ggcggggccg
      901 atccagggtt cgacttaccc cgacctccgg tttggggcgg gccggcgggt cggagagatg
      961 ggcttcgacc tctgtcccat aggggccgtc gtccccctga tggaggccta caggttcaga
     1021 gacctggtgg aggtcgtcgc cgcctcgaag caggggctcg gcccggcagc tccggtccac
     1081 ctcttcggag cgggccaccc catgatgttc gccctcgccg cagccctcgg atgcgacctc
     1141 ttcgactccg ccgcctacgc cctcagcgcg aggcaggggc gctacctcac caccagcgga
     1201 accctccacc tggaggagct gaagtacctt ccttgctgct gcgaggtatg cagagaccgg
     1261 gcagcgcagg atctggcgga ggacccggag aggacggagc tcctcgcccg ccacaacctc
     1321 tccgtctccc tggcggaggt ccggcgggtg aggcagtcga tctgggaggg gtccctctgg
     1381 gagctggtgg agaggagggc gaggtcccat ccgaagatga tcgatggcct gcgggccctc
     1441 ataccccacg ccggctggat cgaagccctc gaccccgcgg cgcgatccac ctgcttctac
     1501 ctggggccgg agtcggcggc gaggccggaa gttctgaggt atggggcccg aatagagagg
     1561 atcgagatat cgggaagggt cttggtggcg gatcgggggg tccaagagga tgggttcgac
     1621 cagatcctct ggtttgtgcc ccccttcggc ccataccccc aggagctggc ggagacggct
     1681 cccctcaacg ccgaggtccc ggcggaggcg gacggggcgg ggatcgccca ggccctggca
     1741 aacctcaaga ggctgatgga ggcaaacccc tccgccacct ttgagatacg gctgaaggac
     1801 ctgcccgggg gatgggccga cctccagggg agggggagat gacccgccac ttcgaggtga
     1861 aggaccgcga cggccccgcg aggatgggaa ggctccgcct cgcggccccg gtcgagaccc
     1921 cggcgatcat ctcgagggac ctgatccagt cggtgggacc gatctgggcc ttcccgtcga
     1981 tcgaggaggc ggtctcggcc gggtcggcgt cctccgacga ggggggcagg atattcatcg
     2041 gaccccacgc ccccttctcc ctccacaccg agccgaccct ggccgtccct ccgatcccga
     2101 ggggcggacc ttcaggagtc gtcgtccacc ccctggcgat ggaccggccc ccggcagccg
     2161 acgtctacgt cctcggggcg gcggggtccc tccgaaaccc gagggagctt gtggcctcca
     2221 tcctcaagat cagagagatg accccccccg actcggccct ctacgccccg gccctcgcga
     2281 cccccgccaa cctcgccctt ctcgtctacc tgggggtcga cctcgtggac ggcgcgagga
     2341 tcgtcgtcga cggcctcctg gggaggtacc acctccggga cggggtctgg cccgtcgagg
     2401 ctctgaagga gctcccctgc cgctgcccct tctgcagggc cctggaggag ggacaggatc
     2461 cgaagctcgt ggcagaacac aaccagctga agctcgagga ggagctctcc atggtgaaga
     2521 cgatgatccg gtcggggacg ctccgcgagt acgccgaaag acaggtgagg gtcgcccccg
     2581 aggagacggc ggccctgagg ctcctcgacc aggagcagcg gtatctggag cggaggaccc
     2641 ccaccgccag gaggagcgtc atgtacgcga actgtgccga gtccctccag cgggtggaag
     2701 tgacccggtt cgccgagcgg gtcctggaga ggtacagggc cccggagagc gacgtcctcc
     2761 tcctcctccc ctgctcggct cggaagccct actcgacatc ccggtcccac cggctcttcg
     2821 cctccgccct tggcgaggcg aggcggaggc tccacgagct gatcctcacc tcgcccctgg
     2881 ccctcgtccc ccgggagctg gaggaggtct accccgccgc gagctacgac gtccccgtca
     2941 ccggtaggtg ggaccttatg gagcgggagt ggctgacgag gtgtcttgac gcttacctcg
     3001 agcagaaccg ttacaggagg atcgtcgccc acatcgaggg ggagctggag gaggcggtcg
     3061 tggatagcgg gatcgacgcc gtctacacgg gagggggcac cggcgccgag gctctgcaga
     3121 ggctctcgga ggcggtcgcc gaggccacct gcgacgcgag acccctggag gggtttttga
     3181 tccagaagtt ccgggccctc gccgactact ccttcggcct cggagccggc cgcgaccttc
     3241 tcgcgggagg cgcgcgggtg agggggaggg agctccagtc ggatgatagg aggaccctgg
     3301 ccgccctaac ccccaacggc accatcgccc tctcccctga gggggcgagg aggcttctcc
     3361 cccggggcgg atacgtcgtc gagatcggcg actttgtccc taagggggac gtcctcgccc
     3421 ccggggtcgc cagagccgac gaccagatcc ggcccgggga cgaggtgatc gttacgggcg
     3481 agaaagcctt cggggtcgga agggcgaaga tgagcggctg ggagatggtc gaggcgacga
     3541 gagggatcgc cgtcgagctc cgccacgtcg agaagatctg aggagaggga gaggagaagt
     3601 aggaagaatg aaggggagga cgtcgcttac caacggggag agagcggagt acttccggag
     3661 gagctacacc gccgtcgatg gcctctggtt catgaaggtg gaggagatga ggggctttga
     3721 ggaggcgctg gaggcggacc gccgggtctg gtccgtcctc cccaagatcc aggtccggac
     3781 cttgaggacg atgctgaagg ccgacgaggg gatagagggg ctcggccagt gcctcgcagc
     3841 gaagctctcg gcggaagggt tcgattttga ggccaagatg gacgaagacg gccgcctcct
     3901 gaggctgatc atatcaaagt gcccatggca caagcttctc gcgagatccg gccgagaggc
     3961 cctatccgag cgggtgggga cggagatctg caacgccgag tacgccacct gggcggcgga
     4021 gttcgagggc ggcgacctga agatatcctt cagcctgaga tcgcagatct gcaaaggtga
     4081 ccaggtctgc accttctgtt ttgaagaggc ggggagctcc gaggatccct gaagacgggc
     4141 atcatccggg gccctataga ccgatgccag cgggtccagg ggcaggagag ggcacaactc
     4201 aggcagaggg agatatgaga tctgtacttc atatcggtag tcgagaggcc ctcaagattg
     4261 cccctctcag actataatag taacgattaa acattataaa tttaacgatc tgctccatcc
     4321 accaaacgga ggagaaatag ggaggcgtgg acttgcggcc ctccatgaag agagggctag
     4381 aacgcccgcc aggggctgcg aggcgggctt ggcgttgtat ctgcttggtt ggttagaggt
     4441 ccccccggag gaggccggtc cacttagagg ccccaggaac cggggggaag ggatgggctc
     4501 agaagtggcc ccatcgtcct ctcacgaccc cgccctcctc gccagcggga acctgtccgt
     4561 gttcctacca cctgggatgg tccggggtcc ttcgcagatg ccatcgccat cggcgtcatc
     4621 gcaaacgtca ggcccccaga ggttccccct cgacccgtcg tcccagaggt tatccccctc
     4681 gtccctcgcc gcctcgccgt ttccagagag gtcgtttcca tagatcgagt tttcgatgga
     4741 ggcgaagtcg gcgaagaccc cgaggatgct atcgaagatc aggtttccgg ttatgaggtt
     4801 ccgggttgct ccgacgagga tcacgcccac atccgaggag gatacgttgt ttccggtgac
     4861 ctggttctcc ctcgacccgc cggagatgtt catcccctcg gtggcgtttc tgatcctgtt
     4921 ctccgtgatg gcgaggcctg aagaggactc ctcgacggat acgccgacgg ggctatcgaa
     4981 gatggtgttg ttgtaggccg aggctccgat ggtcgaccag aacaagatcc cggtcttctc
     5041 cccctccgag atggtattgt tattgacctt cgtatagtta gaccggtcga ggaagattcc
     5101 gtgatcgctt ctggtgacgt tgttcagatc cgcggtgttg ttgtggctct ggaggaagct
     5161 gatcccgttt ccgtggttgg aggtgatgtt gttgtctatg atgttgttct tccaggtctt
     5221 ctccagccgg accccgtctc ttccgttccc ttcgaccctg tttccgatga ccaggttatc
     5281 gctggactcc tcggcgccgc agggcggctc gctgcaatac tcccagaaga tgctctggat
     5341 gtaaacccca tggtcccggt tgaaggagat gacgttgttg gcgatgagcc ccgtcgaccc
     5401 caccagatgg attcccttgc cgttcccctc aaactcgctc tcaaagccgt ttctcgatat
     5461 gatgttcccg gtgatgaagg tgaagttggc gtttacggcc cagatcccgt cgttgacgct
     5521 ctcccggatc tcgttctccc tgaagatgtt cgagtcgctc ctctgaaggg agatccccca
     5581 gttgttgtga atgagggagt tcccctcgat ggtgttctca ggagagttgt acatggttat
     5641 gccgtagccg aaagagacgt ccttgatcgt atttccgagg atctggttgt tgctggcgtt
     5701 gatcaggcct atggcggtca ggtcgtttcc ttccaaggtg ttgttgacga tcttgttgaa
     5761 gtcggagtag acctttatcc cggcgtggcc gatgacgtcg ttatgctcgc cggtgtcgta
     5821 cggcgtcggc caggtggcgc cggtgatgac gaagttctcg acggtgacga aatcggccct
     5881 tatggctaca gcggtcccac cgctcatctg caccatctcg gtcaatcccc cggattcgct
     5941 ctcgatatca gcggtggggc ttcccgcccg gaggacgggc ataccgccgc cggtgtcgtt
     6001 tccccggagg gtgagaaagg gggtggttat gttcaggttc accaggtact cgccgctgtg
     6061 aacctcgacg acgtcgccga cgttcgccgc ctcgacggcc ggaccgattc gataaaagtc
     6121 gcagccatcc tccccgacgg tgatcgtcgc cgccagggac ggcggagatg atatcaggat
     6181 gaagatgaac gcagctataa gacggctcat gggatcacct cgataggtta tcctctttga
     6241 gaataaatag aatatggaag atccgattcc tttggagccc tctcctcccc cgggcggaga
     6301 ccatgccgcc cctcccccgg ggatcgatgg acctcgccgc tggttctggt cttcggcctc
     6361 ggagcgggag ggctcatgcg aggctgaggg atcggtcatg caggcatcac cgaagcattt
     6421 ttagtcgtcg gagacagtat cgatatccat ggaggggggt aaaataggaa agctcatttt
     6481 ggcgctgctc ttcctggcgg cggcaacccc tgccggggcc accgtggtgg acgacctcat
     6541 cctggacctg aagtacggcc aggccgacgt ccgggcgggg gcggcccgcg ccctcggaga
     6601 ggccggggga gcaggggccg tggaaccgct gatatcggcc ctggaggacg aggccgccga
     6661 ggtgaggtgc gaggcggcgg cggccctggg agaggtcagg gacgcccggg ccgtcggacc
     6721 cctcatcgcc gccctgaagg acccagatcc gggggtcaga agagaggcgg cccgcgccct
     6781 gggagagatc ggcgaccccc aggccataga gcccctcgcc cacctcgccc tcaaagatcc
     6841 ggacggcggg gtgagagaga aggcggtcga ggccctcgcc gaggccgggg atcccgccgc
     6901 ggtaaccccc ctcatcggag ccatcaaaga cgaaaactcc tcggtccgga tgcgggctac
     6961 ccgggccctg ggggagatcg gcgaccccca ggccatagag cccctcgcct acctcgccct
     7021 caaagatccg gacggcgggg tgagagggga ggcggtcgag gccctcgccg aggccggggg
     7081 tcccgccgcg gtaacccccc tcatcgccgc cctgagggac gccgatccgg gggtatccga
     7141 gaaggccgcc gaggccctcg ttatcatcgg cgatccgagg gctgtggcgc ccctcatcga
     7201 ggcccgccgg tacgacgagg cgataagcat cgaccccgcc tcccccctcg cctggggggg
     7261 gaaggggcta tctctatacg atggggggag gctctcggag gcggtcgagg cctatgatac
     7321 ggctttgagc ctcgaccccc aggaccccta cgtctggaac aacaagggcg tcgccctccg
     7381 ggacctgggg aggctctcgg aggcggtcga ggcctatgag gtggccatcc gcctagaccc
     7441 ccagtactcc cacgcctggt acaacaaggg gatagccctc gacgagatgg gagaagagga
     7501 gaaggcgatc gaggcttatg atatggccat cagcctcgac cccgaggacg ccaacgcctg
     7561 gaacaacaag ggcgtcgccc tcttcggcca ggggcggctc tcggaggcga tcaaggctta
     7621 cgacgtggcc atcgtcctcg accccggata cgcccaggcc tggaacaaca agggcgtcgc
     7681 cctctacgac caggggcggc tctcggaggc ggtcgaggcg tacgatatgg cgttgagcct
     7741 cgaccccgcc taccaccacg cctggatgaa caaggggctc gccctcagga gcctggagcg
     7801 gtacgaagag gcggtcctga tctacgacga ggtgatccgg cagaggcccg aggacggcga
     7861 cgcctgggtg gggaggggcg cagccctcca ggccctgggg aggggggagg aggcggaggc
     7921 cgccttctcc aggggccggg agctgggagg ctagggaagg cggtggcgcc ggaggagctg
     7981 agtttcatgc tggaccaggt aggtccaagt caggcgaggc tctggtacca gcgctccgcg
     8041 gcggacccct atgtcctctg tgtcgagacg gcctataccc cttgaggaat tggcggccat
     8101 ccataaaaag acccaaacct ctcttgggtg ggttgtctga cgccccgaaa atttattaag
     8161 aataccctcg aagactggac cataatgaag gactctaccg atcataatga ggtcgcatgg
     8221 aggggcggat cgaggctgct caaagccagc aatccagtaa tgaaaagctt ttatgccgct
     8281 ttatcaagtt atatatcatg ataatccgat ccgccttcaa gccgacggtc cttcttcttg
     8341 gactgatctt attcggatct tcgccctccc tagcccagtt cggagggtgg gatagtccgg
     8401 gggatctggg cccctgggaa gaagagggat cgtcatcctt cggctggacg gagccgccac
     8461 cttctgagac ctccgagacg ggagaagccg acttccccag cagcatcctg gagagccccg
     8521 agaccttgat cgaggagggg gggtctttcg agtacagccc ggcctcatcc ccggctccga
     8581 ccgtcgcccc cttctccatc gcccccgatg gaggccagaa cgctttctgg atcgtcagcg
     8641 aggacggtac ccgctactgg cgggccgtaa acatccccgt ctaccactat gcaaggctcc
     8701 ttatcatccc ctcatcctcg gggtcgctga tggtggagaa gaggacccct gcgggcctcg
     8761 ttgagacccg ctacctgggg aacgtctggg cgcataatca atataggaca tggttctacg
     8821 ccgacgcccc ggggatccac cagctgaggt acaggatagg aaacggcccc tacagcgacg
     8881 tattgacctt ctacgtcgga gccgccgtcc ccgttccgcc acccgccccc gacgggaggc
     8941 ccaacatcca gatcgtgacc caggcggtgg acccccagac taaccgggtc tactactcct
     9001 acaacccgca ggggctccag atcttcagga tcaaagtcct ggtgaccggc cccgacctct
     9061 acaggatcag gtccgtccac taccagctcc acgccacctt cagcccgtcg gagcagacca
     9121 tgacaaaccc gcgagacaac ttcgagctgg agctatggac ctggggggcg ttcaacatgc
     9181 cgatcaccgt gacgatgacg gacgggaggg catacaggta cgactaccgc ttcaccttcg
     9241 gagatcagtt gagggacgcc cagaggagag gggtgccctt cgtgagggtc tggtaaggag
     9301 agaggcggca gatcggaagg agcgtataaa tcgtccggga tcattctgcc gacgaatatg
     9361 aggatccaca cccttcagca cgtccccttc gaggggacgg gatcgatcgg agaatgggcg
     9421 gtggagcggg gccacagcct cttctggacc cgcctcttct ccggcgaagg cctcccggac
     9481 ctgggggagt tcgactggct catcgtcatg ggcggcccca tgaacgtcta cgaggaggag
     9541 agatacccct ggctcgcccc ggagaaggtg ctgatccgaa gggcgatcga tgagggaaag
     9601 gtcgtcctgg ggatatgcct cggggcccag ctgatcgccg acgtcctcgg cggccgggtc
     9661 aggaagaacg ggcaccggga gatcggctgg catcccgtca acctcacctc cgaggggtcc
     9721 aagtccgacc tcttcggcct cctccccgag gagttcttcc ccctctcctg gcacggcgac
     9781 accttcgaca tccccccggg ggcggtccgg atggcggaga gcgggggctg tgcaaatcag
     9841 gccttccagc tcggccgggc catcgggctg cagttccacc tggaagcgac ggcggaaggg
     9901 atcaggagcc tcatcagaaa ctgctctgac gagctcgccg gcggcgggag gttcgtccag
     9961 gccgcagagg agatcctggc aggcttagaa gccgtaccgg agatcaacgg gctcatggac
    10021 cgatttctcg acggggtaga gaggctatac ggcccatgag gggcctctcc gctgaggagg
    10081 gctggtaccg gacgtggatc tagcgcctca gagccgcggc gaggatgatc ccgaagagga
    10141 aggctaggcc gacgttggtg aataaggcca gaagggcgat gctgccggtg aggaggggcg
    10201 catccgtccg gaggccgcac cgtcccatct ccagggcgac gaagaggagg agagccccga
    10261 agaggccgat aggaaggatc gtcagggcct cggcgccggc gaagaagagg gcgacggcga
    10321 ggaggacgag cccccccatg acggtcgaaa ggccgctcac cgccccgaag cggtggtggg
    10381 cagcgagccc ccccgccccg tggcagatcg gaaaaccgcc gaagggcgcc gagacgatgt
    10441 tcatgatccc catggtgacg cagagcctgt cgggctcgat cctcttcttg aagaggtcct
    10501 cggcgatgag ggcggtcgcc accgtggcgt tggtgagggt taggggggtc tgagggagga
    10561 ccagatgcca tccggcccag atgaaggtcg ccgggtcggg gacggggagg accccgatcg
    10621 agatgagctg gatatcgggg acgcctctgg tggagatgcc gtaaccgatg cccaggccga
    10681 agacggcgag gatggatacg tccgggagac ctctccagct cctgaccagg aggaataaaa
    10741 ggaagatccc cacggcggcg gccgcaaata tgaggtcctg gaccatcatc cccgccgagg
    10801 tcttgatgag gatgaggccg agccccagct ggaccccgcg gacgacgccc tccgggatca
    10861 gcccctgcag ccgctcaaac ccccgaaaat accccagggc gaggagcccc acccccagga
    10921 tcagccccga ggccgcgatc tccccggagg tcagctcccc ggcgatggcg atggccccga
    10981 tcgccttcat cggctccacc gacatgggaa tcccgtaatg gatccccatg acaatgtacc
    11041 ataggccgat gaagaggagg gcgggcccca ggtctacctc cgatacgagg gcggccccga
    11101 ggaggatggg aaggaccgtc ccgaagtttc ccatcgatcc ggagatctcc cggaggatcc
    11161 ggttggaagt ccataaggga tctttcatgg aactggggaa aggcataatc ccgccagcta
    11221 tatctcgatg acgccgtggc agccttcgcc caagagatct tctattgtat cgagatgatg
    11281 caggcaggag gaagccttct tacgacccgg atctgccgga gccgcctttt gggccgcctc
    11341 cgatccccat ccagcagagc gaaaactctg ttcggctgta aagagccctt cggcggccca
    11401 taatggatta tattgccata aatgaaattt atcagttcgt atattgtttt aatgatgatt
    11461 cataatttgt attaatttgt attcagaaat ctcaaatact tccatataag cggggatggc
    11521 aaaaatgcca taagagttct ggaggtttgt ggcgaggtga atcttccgac gagggcgatc
    11581 cgggccagag gctgcaagat ggaggaggat ccgccgggga ggggagagga ggtcgtcgac
    11641 ttgcccctcg gcgatcctga gaccgagccc tcccagagga cgggatcatg aggggagggg
    11701 aggggttcca ggagaagggt ccggagaagt tcttggtcac catctccttc gaccgcgaat
    11761 ccgcgggcat attcagggag atgaaggatg ggctggtggg atcccagagc gagctattga
    11821 ggacggctct gaggttttac agcagacaca aagccctctt cgaatccctc gacggtgatc
    11881 ggctccgcat ctatacggag atgctctcgg agggggagca cgtcatcctg gacattgacc
    11941 actggatact ttttttgagg ttcatcgagt cgcaccctga taaagagaag ttctgggaga
    12001 tgcatcaaaa ggtatcccag gcccatgccg aggagttcat acaaaagagc cacgatatta
    12061 agacggtact ccggaggctg gaggcctgca acctcttcaa gctgatcagg acctctgaga
    12121 cggaattaac cctgatcctg gggtacgacg tcccgaagag gttcatccgg accgagatcg
    12181 aagagatcat ctccaagatg ggaaggaggg ccgagataag agaggacctc tcgaagatca
    12241 gggttgagat cctgcccccc agatgatcga tcaaaggaga tgatcggacc ggaggcgggg
    12301 gccccttcca gtagagatcc cttcccggga gcctcctctt ccccctttcg gctccgcaga
    12361 gcggctctcc tcgggcggcg gtcaaatata acgaatcgcc agaccgtcct ggatggccca
    12421 ggagctgaga cggggggata ggggagatgg tctaggctcc tcttttctca gatcagggca
    12481 taccgggtaa gctcttttca taatacctat gaagctttat caaatctctt tttaaatccc
    12541 cagggtccca ttctgccttg taaaacgggt cggacgcgtc gatggcaaat tccagacgcg
    12601 aacctggaag tttacgggtg gtaaataatg gtacttgaca gcggcgatac ggcctgggtc
    12661 ctggtctcga cggcgatggt gatgctgatg ataccgggag tcgggctctt ctacgggggc
    12721 ctcgtccgga agaaggacgt cgtctccatg atcggcctct ccttcctggc cctggccctg
    12781 gtcagcgtcc agtgggtgct ggtcggctac agcctctcct tcggggcgga cgtcggaggc
    12841 ttcatcggcg gcctcgacct cctgggcctg aagggcgtgg ggatggacgc cggggagggg
    12901 acgatcccgg gcctcctatt catggccttc cagctcgtct tcgcggggat caccctggcg
    12961 atcataacct ctgccgtcgc cgagagggtg aggatcggct ccttcatcgt cttcggcctc
    13021 ctatggacga ccctcgtcta cgaccccctc gcccactggg cctggggcgg cggctgggcc
    13081 ggccagctcg gggccctcga cttcgccgga gggacggtcg tccacatcag ctc
//
LOCUS       DASM01000128            6645 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_108133, whole genome shotgun sequence.
ACCESSION   DASM01000128 DASM01000000
VERSION     DASM01000128.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6645)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6645)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6645
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_108133"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1291 a         1915 c         2107 g         1332 t
ORIGIN      
        1 cgtagataag ctgggggcga aagcaaggtg agtcgccgca gttcgaggat gataacggct
       61 gcgggtgaaa tgaggccttc gggcccggag gcttccagcc ctaaacgctc aatcagaaaa
      121 atatgatcct gcccccccgt ccggccagaa agctcgccgt aatcggggca cctcacccct
      181 tgaggaggta cccgccgtcg acggggagtg cgaccccggt gatgaagctc gcctcctccg
      241 ataagaggaa gaggacggcc cgggatatct cctcgggcct gccgagcctc cccatggcgt
      301 gggcctccac cagggccgca aacccctcct cccccatctt ctcgaatagc ggggccgtca
      361 gctccgtggc gacgtagccg ggagagacgg cgttcgcccg gatcttctcc cgggcgtagt
      421 cgagggcgat ggactcggtg aggccgacga ccccgaactt ggaggcgttg taggaggtcc
      481 gggtccggaa ggccttcagc cccccgaccg acgatatgtt gacgatggac ccgccgccgc
      541 cggcgagcat ttgaaggact gcggccttac agcagttgaa aacccccgtc aggttcacct
      601 ccagggtcat cctccagtcg tcgagggccg tctccgtgat cccctttcga gaggggttga
      661 tggcggcgca ggtcacaagg ccgctggcaa ggccggtcag gtcccttatc gaatcgaaga
      721 cctcatccac ccgatggtgg tcggagacgt cgcattcgtg gcagaagaac ctctcgccgt
      781 cggagggggc tgagcgtgag gaggcttctc tcttggcttc gaggagcctc tcccccctcc
      841 gccccaggat gtggacatag gccccctcgg cgtggaggtc taatgcgatc cgctttccta
      901 tgccggtccc gcccccggtg acgacgacgg tcttgctggt gaagcggcac cgtctgctga
      961 gaagatcatg cgacatctct tgttattata atggctgcgg ttatatagga tatgctgagg
     1021 ctgctgatgg cgagaggctg aggatccgat actgatcggc cgaggcgatc gccttaagat
     1081 tcggagggtc aaggggccgc catctccgcg gcctaagccg ggatgggcgg ggatgaggcg
     1141 ggaaggccca gacccggaat tatgcccgcc tccgcctgga ggcagagctg ccggcaaggg
     1201 tgggtggggg aaccgagcgc cacccggcag atccatttat cttcacctcc gaatatagaa
     1261 tcccccatgg acgccgagct taaagaagcc ctcctggaac gttgccgcga gatggagatc
     1321 cccctggtgg gggtcgcagc ggcagaccgc tgggctgatc cgcccttcga gccctgggtc
     1381 cccggtgagt tctggcccca ggcgatatac cccgagacga ggtcggtcgt cgtcatcggc
     1441 cttcccatat cccttcccgc gctggagacc tctccctccg tatggtaccg ggagctctac
     1501 cggaccgtca acatcctcct cgaccagtac acctaccgga tcggtaactt cctcaacgac
     1561 cggggttttc cttcggcctt cgtcccccgg gacgggtacg ccgacgtcga ggcgctcctg
     1621 aagaaccccg tcgccttctt ctcccaccgg cacgccgcct accttgcggg cctggggagc
     1681 ttcggggtga acaacgtcct cctgacgagg aagtttggcc cccgggtccg cttcggctcc
     1741 gtcttcacgg cggcggagct tccgcctgat cctgtgatgg aagaagacct ctgcacccgg
     1801 tgcatgaggt gcgtccagct ctgccccgct ggcgccttgg acgatcggga ctacccggca
     1861 gggatcaccg acaagagcgc ctgcaccgcc tacagcgcct ccttagcgaa gcggggggtc
     1921 tcgccctgcg gggtctgcat caaggtctgc cccgtggggg aggaccggga gctctacggg
     1981 aggagggatc cctcgatata tgccagaggc gatgggaggg aggatctccg ccggggctgg
     2041 gagcacgtcc agagctacgg cggaaggtga cggcagaggc cgccggtggg gatggcaccg
     2101 ccctcaggcc gtctccgtcc tcgccacctc ttccaggccc agctctttta tcgactcctc
     2161 cctcatcttg tacttctgga tcttgccggt gacggtcatg gggaagccgt cgacgaactt
     2221 gatgtaccgg gggatcttga agtgggcgat cctccccctg cagaacgcca acacctcctc
     2281 ggcggtgagg gtcctcccct ccttcagctt tatccaggcc atcagctcct cgccgtacct
     2341 ggcgtcaggg accccgatca cctggacgtc gctgatgtcg gggtgggtgt agaggaactc
     2401 ctcgatctcc cgggggtaga tgttctcgcc gccccggatc accatgtcct tgagccggcc
     2461 ggtgatcttg cagtaatcct catcatccag gatccccaga tctccggtgt ggagccaacc
     2521 ctctttatcg atcgccttct cggtggcgcc ggggttgttg tagtagcacc tcatgatggc
     2581 atacccccgg gcgcagatct cgccggtctc cccccggtgc atgatcttcc cggtcttggg
     2641 gtcggtgatc ttcacctcag tgtgaggcat cggctttccg acggtggaga ccctccggtc
     2701 cagggagtcg gatgtggagg acatggtgat cccaggggag gcctccgtct ggccgtaggt
     2761 gatcaccacc tccctcatgt tcatgaggct gctcaccttc ttcatgtact ccgtcgggca
     2821 gggcgagccg gccatgatac cggtccgcag gctcgacagg tcgtacttat cgaagtcggg
     2881 gtgctccagc tccgcgacga acatggtggg aacaccatga agggcggtgc accgctcccc
     2941 ctcgacggcg gcgagggtcg aggcggggtc gaagtactcc gccgggagga ccatcgtcgc
     3001 cccgtgggtc atgcaggcca tgttcgagag gaccatcccg aagcagtggt agaaggggac
     3061 ggggatgcag agccggtcct gatcggtgaa cttcatgcac tcgccgatga agaagccgtt
     3121 gtttaagatg ttgtggtggg tgaggacgac ccccttgggg aagccggtcg tcccggaggt
     3181 gtactggatg ttgatggggt cgtcgaagtc gaggctctcc tgctccgccc ggaggaggtc
     3241 gtcgccgacc tcctcgccca tcttcaggac ctcctcccag gtgcgcatcc ccggcctctc
     3301 ctcccctctg atcaagacag ccgtctttag gaaaggcaaa ttctcgcagg atatcttgcc
     3361 gggctctgcc tgcgccgatt ccgggcagat atcgcggaac ataccgacgt agtcggaggt
     3421 cttgaaccgg tcgatgagaa agagggcctg ggcctccgac tgcttcaggg cgtacaacag
     3481 ctcgtgggtc cggtaggcgg ggttgatgtt caccatgatg atccccacct tggcggtggc
     3541 gaactggcag atgacccact cggcgatgtt cgtcgcccat atggccaacc ggtcgccctt
     3601 cttcaggccg agggcgtgta atcccttagc agcccggttg acctcctgtt gaagctcgcg
     3661 gtaggtgtaa cggagcccct ggtggatcga gactatggcg tcgttgtcgg ggtacctttc
     3721 ggcgatctcg tcgaacatgt ccggtatcgt ctttccaatc aggggcttct cggaggccat
     3781 gaaggcgtag ctttgggatt tactgcccgg catgaaagag gcgtgagatc cttattcgca
     3841 tttaacctta tcccggcggc gcgggtggag gaggattata aataattcga ggtataatta
     3901 gaaaggactg ttcagctcac cgccaatatt ttcgaagccg ccttcataaa tgatctccga
     3961 gaaaagcaac cgaggcggcg cgacctcccc gatagggagg gatcatggag cgccttcgag
     4021 ggcgatcgtc ttgagaagat cgagggaccg cttccggcct gatccgggga atttcgcaga
     4081 cctacaggtg ggcctacctc gacatgctgc ctatattgat cggcgttgcg gcggggaggc
     4141 agaggcgatc ttcttacgaa acgtcatcga gttcatccat gacccgttct tcttgggggt
     4201 cgatcaaata aaacgaaaat tttatatatg ttgacctgca aattagtcga aaatttcata
     4261 tattaagcaa taggaacata ctgctttaag ctgaatcact tgccatagct cagcattaag
     4321 aggtgagatg atcatgcgta atttttggaa ggctatgatg gccatcatga tagggatgtt
     4381 tgttatcatg ccggttcacg gcagcggtgg aagcgacttt gacggcgacg gaaaggcgga
     4441 gatcctaatc tcaagcccct ggggaatcgg catcctgaag atggaaggaa gtgctctgag
     4501 cgcccccgtc atggcggcaa acggcgccag gttcccgggt gggtggctcc tgaacaccgc
     4561 tgacaacaaa ttcgagatga aggatgactt tgacggcgac ggaaaggcgg agatcctggt
     4621 ctcaagcccc tgggggatag gcatcctgaa gatggaaggg ggggctctgt ccgctcccgt
     4681 catggcggcg aacggcgcta gattcacagg gggttggctc ctgaacaccg ctgacaacaa
     4741 attcgagatg gcggcggact ttgacggcga cggaaaggcg gagatcctca tctcaagccc
     4801 ctggggaatc ggaatcctga agatggaagg gggggctctg agcgcccccg tcatggcggc
     4861 aaacggcgat aggtttccgg gagggtggct cctgaacacc gctgacaaca gattcgagat
     4921 ggcagaagac tttgacggcg acggaaaggc ggaggtccta atctcaagcc cctggggaat
     4981 cggaatcctg aagatggaag gaggggctct gagtgccccc gtcatggcgg cgaacggcgc
     5041 caggtttccg ggcgggtggc tcctgaacac cgccgacaac agattcgaga tggcagcgga
     5101 ctttgacggc gacggaaggg cggagatcct catctcaagc ccctggggaa tcggcatcct
     5161 gaagatggaa ggaagtgctc tgagcgctcc tgtcatggcg gcgaacggcg ccaggttccc
     5221 gggtgggtgg ctcctgaaca ccgccgacaa caaattcgag atggcggatg actttgacgg
     5281 cgacggaaag gcggagatcc tcatctcaag cccctgggga atcggaatcc tgaagatgga
     5341 agggggggct ctgagcgccc ccgtcatggc ggcaaacggc gataggtttc cgggagggtg
     5401 gctcctgaac accgctgaca acagattcga gatggcagaa gactttgacg gcgacggaaa
     5461 ggcggaggtc ctaatctcaa gcccctgggg aatcggaatc ctgaagatgg aaggaggggc
     5521 tctgagtgcc cccgtcatgg cggcgaacgg cgataggttt ccgggagggt ggctcctgaa
     5581 cactgaagac aatcgcttta tttagggggg agctagcggg gcaggtttcc aggagggcgg
     5641 atttaatcaa gctttttggg ggacaccctg ccacaaatag ggcggggtgg cagcacccca
     5701 agtttttttg atattttccg aaatcaccga gtcttcgttt aggttttggg tgaatgccat
     5761 ctaaacaaga cccctaattc aatttcatgc actgcattat tgcgatcaaa tcggattagc
     5821 cacactttgg tgattttcaa cggttcgcac ccacgaattt cgtttgctag attcctttaa
     5881 ttagcatgac ttagactgaa cctccggttt cccctgagac tgcctaatat gtgattgaag
     5941 gcgcgggttc acttcatctc ccacctaaag gtcggggtat tcgtgaccct ggccaccggc
     6001 cactttgcag atctggcggg aatcgagatc gatcccaggg ttatgaccct tagaaaggat
     6061 tggatgaaga atcccattcg actcgctcct tatacatcgc gaagccgcct ccataactga
     6121 ccgccgggaa aagcataaga agcggcgcga cctccccgat aaggagggat catggagcgc
     6181 cttcgagggc gatcgtcttg agagaagatc aagcggcctc tggagggctc atccggaggg
     6241 cttttcgaaa gcgccggtgg gcctacatcg accttttggc gatactgatc ggggtcgtgg
     6301 gaggagtcgg ggcgatcgtc ttcgaccgcg ccatcgacct cgtctccagg atattcttct
     6361 ccgggatcga tccggcagcg gggccgggcg tctccgtcat actatatccg atcataggcg
     6421 gcctcatcgt cggccccatc gtcttcatcc tcgccccgga ggcgaagggg cagggggtct
     6481 ctcggatcat cgagagcgtc ctggtgggcg gggggcggat cagaagacgg gccggattta
     6541 tactcatcct ctcctcggcg gtgacgatag gaagcggcgg cagcgccggg agggagggtc
     6601 ccatcgccca gatcggaggg tccttcggct ccctcgtcgg ccaga
//
LOCUS       DASM01000129           10191 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_48209, whole genome shotgun sequence.
ACCESSION   DASM01000129 DASM01000000
VERSION     DASM01000129.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10191)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_48209"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1704 a         3259 c         3288 g         1940 t
ORIGIN      
        1 tctggaactg gatggcgtaa tcgatgccga ggccgatcag gatcgggaag gcggccatcg
       61 agaccatggt cagaggaacc tggaggaatc ccatcgtccc gaaggtccat attatcccca
      121 aaaagacgat cggtagaggc aggaggggca ggttcacgtg gcggaagacg aggaggaggg
      181 ccaccaccat cagcaggccg gagatgagga ggagggttcc catggaggtg ttcatctccc
      241 gctcgatctc gtccccgagg gcgacctcgc cggtgactat cgtatcggcc ccggcgggga
      301 actccgccat cttgacggcg gccctggcca ccggcaggac ggcgtcgatc ctctcctgag
      361 ggatgaagga tggaaggtcg atggagatga tggtatggct ccggccgggg aggatcgcgg
      421 agatgacctc ggggttcgtc ccctccagcc ccgccaggat ctcgtcgatc ctcccctggg
      481 attcggggac cctccggacc cccgtctccg aggcctcgac ctccatcacc agctcggcga
      541 tgctgaccgt ccccaggacg tcctccaccg tctccatctg gcgggtcaac cgttccatcg
      601 ccttcaggga ggctggggag gtgacggagt ctccctccac caggacgacg atcgactggg
      661 tcccgaacct atcgttgtag aggtgggcga actcgacgta gagcctggag tccgggtcta
      721 cgaaggtctc cgtctccgtc tccatggtga tggtctgggc atagtggatc gaggccaagg
      781 tcaggaggac cgccgccgtc aggatgacga tcggattggc ctcgatccat ctgcctagcc
      841 tctcagccct attcgtgatc ttcacctccc gccgctattt ctatattttg cattatttcg
      901 aaaattcgag tctttcggat gaaacgaaat gtacttaaag attgtggctg actcttcacc
      961 gatgatccgg gaagatttca cccccgtaga ggcggcgaaa cggtgcatgg tggacgccag
     1021 catccaggtg gccaccaagc tggggtacag cgacgccgca ggagccctcc gaggagttct
     1081 tatgctggcg gaccaacccc gatcgatgga ttatctggtg gagcagaccg ggtacagcaa
     1141 gtcgacggtc agcaccaaca tggcccatct cgagaggatg ggtatagtcc ggcgggtacg
     1201 acttccagga gacaagaggt actattactc caccataatt cgcatcgacg aggggacgaa
     1261 ggttcacaac gagaacttca gacagatcat gcagatcatc gccgccggga ctgccaaggc
     1321 ccaggagatt ctggagggtg ccgaagaggg ggatgatgct cgctggctca gaagtcggct
     1381 cgcgttggtg agggaggagt gctccaaggc cctcaccctg gccgatctcc tggaccggtt
     1441 caccatcacc gagctgatcg atatcctcaa gctcgaggtg gaagggagga agaagggcgg
     1501 caagtgatag gtgtcgaggt gaacaggata cgacttccag ggacgaaggt ggagttgaga
     1561 tggcgccggg tcaggggatc atcgagggct ggtgccgctc cccatgaaca gagatgaccg
     1621 ggtcttcgac gtcctattca tatcggtctt cgccgccatg ctgggccttg ggatagtcgt
     1681 acccctcctc cccatatacg ccgaagagct gggggcctcg ggggtctgga taggcctgat
     1741 cttctcgggg ttcgccgtcg cccgggcgat cttcatgccg atcgtcggga gggtatccga
     1801 cgaggcgggg cggaagaagt tcatcgtatt cgggctcctc gcctacgccc tcctttcctt
     1861 gggctacgtc gccgccggga gcgtatattc cctcaccgcc gtccgtttcc tccacggaat
     1921 cgcctcggcg atggtcgtac ccatcgccat ggcctacgtc ggagacctct ccgggagggg
     1981 ggaggagggg gcgctgatgg gaaagtttca ggtatcccta tttctggggc tcggctccgg
     2041 ccccctcctg gggggggtcc tgaacgacgc cttcggcttt tcttcggctt tctacgccat
     2101 ggccgcccta tcgaccctgg cgttcctggt gatcgtcctc ttcctcccgg aggcgaagag
     2161 cccttcatcc agagggaggg agcggctctc catccctcgg gccttccgcc atcccggggc
     2221 cccggcggtc atagccttca ccacccataa ctccatctct cggggcctcc tcctcgtctt
     2281 catgcccctc tacgccccca cgatatccat ctcccccggg gaggtggggg tgatcctcac
     2341 cttctccatc ttcttgatgg cgatctttca ggtgccgatg gggaggatgg cggacagggg
     2401 acacgccctc cggatgatcg tcgcgggggc ggtcgtctcc gctggaagcc tggtcctgat
     2461 ccctgccacc aacaccttct gggagctctt catggtggcc gctgccaacg gccttgggag
     2521 cgctcttatc ttgccagccc tgatggcggt cacggtgaag ctgggaagga ggatggggat
     2581 ggggacctcc atgggggctt acaacaccgc catgagcttc gggatgatca cggctcccct
     2641 cctggggggt ctggtgatgg acctgtcttc cattgggttc gtcttcatcc tgggaggggc
     2701 cgcagggctc gcggccttcg tcgccgtcta ccttctgacc tggagatctc tggcggcggt
     2761 ggaggcctga aaaaatgatg tgagatccgg gggtcttgtc ccggcgggtc ggaccctcag
     2821 gcggggtccg ccggcttggg gatggtgaac ctgaaggcgg ctcccctgcc gggggccgac
     2881 tccgcccaga tccggccgcc gcaggtctcg acgatcttct tgacggtggc aaggcctatc
     2941 cccgaaccct ctccagaccg ctctagcctg gtgaaggggt cgaagatctt ctcggcgtac
     3001 ttcatatcga agccgaggcc atcgtccttc acccagaact cgtactcatc gccccgatcc
     3061 agggagccca cctcgatctc gatggcatcc ttggagtatt tgaggctgtt ctccaggagg
     3121 ttggagaaga cctgcttcgc ccggctcctc tcgcagctga tcgtggggaa ctcccccacg
     3181 agggagaggg tcgccggctt ccccttcccc gccgatttga cgtcctcgta ggcctcggcg
     3241 acgagcctct tgaggtccac catctccggc ggcgacttta tcctccctat ccgggagtag
     3301 tcgaggagat cgaggatgag ccggtccata ttctcggcga tcgccccgat ccgctcgagg
     3361 tactttctcc ctgcctcctc gaggtcctcc ccgtaatcct cctggagggc cagggcgaac
     3421 ccccggatcc cccggagggg ggcccggagg tcgtgagaga tggtgtaggc gaagctctcc
     3481 agctcccggt tgagggtctc gagcctctgc tggtactcga tcatctccga tatgtccctg
     3541 gccaccacaa tcgtcgccat cggctttccg tcgctgctgg cgacgggggc gaagctgatg
     3601 gaggcaggaa aggtggagcc gtccttcctg acgcaggtca cccgccgccg gccgccgttc
     3661 ccctcgccgc tggagggagc atcctccccg aagtccagga tgaagtcctg gtggagccgg
     3721 ccccgggcct cctccagccg aaatccgaac atatcaagga gggccgggtt tgcaaagagg
     3781 aggtggctgc tcttgccgaa gatggcgatc ccgtcgttgg aggactcgat ggcgtccatg
     3841 aataggagca tctgctcttc cagccaccgg tcgtaggaga tgtcgtggcc gaaggcgatc
     3901 acccgctcga tctcccccgt cctgctcctg acggcgctcc acccccactg gatcgccatc
     3961 ggctcccccg tcctggtgac gacggccgcc tccgtctgct cggcgggctt cccctggaag
     4021 atcgaggaga ccgccatctc aaacccccgc cgatcggctc cgtcgacgaa cctcatgaag
     4081 aagttctctc cgaacgcctc cttcgccgag acccccgtca ccttctcagc cccccggttg
     4141 aagagggtga tcctcccccg gggatccaga ccgacgatga agaggtcggc gccctcgatg
     4201 atcccccggg agaactcccg ctcggaccgg atctcctcct ccatcttggc gtactcggtg
     4261 gtgtcctggg cggtgatgac gatcccgata ccgttccctt ccccgtcctc gagctttatg
     4321 ccgctccagg agatcacctt ctcctctccc gacctcccca aaatcctcgt ccggcgcccc
     4381 tccaccgtca accccatctc tatctcgtcc agggtcttga ggaactccga cctctcctcg
     4441 ccgtcggaga agaggagggt ccagacctcc tcgtccccca aaacctcttc ggcggagtaa
     4501 cccaaggcct tctcggaggc ccggttccag aggaagatcc tccgatctcg gtcgatgacg
     4561 aatatcatgg ggcttccgat ctcgacgagc ttttcgctca ggtccgccag ctgctttatg
     4621 ttctcctcca cccgcttccg ctcggttatg tcgacgatcg tcccccggag gccgacgggc
     4681 ctcccgtcgc gaatgatcgg cctggagtag acgatgaggt ggaaggtcgt cccctccttc
     4741 cgcaggccag tgtactcgta ggtgaagacc ttatcatcgt gaagacttcg ctggaagttc
     4801 ttcatcatct cttcccggtc ttcgtaggcg aagacctgga agatgtttac gccgacctcc
     4861 agatcctgcc ggtcgtatcc atagagctcg aaggcggtct ggttggcgaa ggtgactttg
     4921 cctgcgagat cgcattcgaa gatcggctgg gggagggtgt cggccatctc ccggaacctc
     4981 cgctcgctct ccgccagggc catctccata tttttccgct cggttatgtc cagggcgatc
     5041 ccctcaacct gggtcacggc accggcttca tcccggctga tccagacgtt gctcgcgagc
     5101 catcggacct cccccgagga ggtgaggacc ctgaactcct ggaagaagct tctggatcct
     5161 tcctcgatct gccgtccgag ttcggagact accagctcca gatcttcggg atggaccagg
     5221 tttctagcaa aggaaacggc tccggaatag aagtcctcgg gggcgtaccc cagttggctg
     5281 acgttttcgg atacgaactc gacggggccg ccgaagggaa ccgcctggac gaaggcgatg
     5341 gccgggctgc ggttgacgat ccgctccagc tcttttcgct tctctgaggc ctccaggatc
     5401 gcccggtccg tcttcttctg ccaggtgacg tcctgcatca cccccgccat cttgatcggc
     5461 ctccccgatt cgtccctctt cacgacgagc ccccggtcgt ggacccaggc ctcggcaggg
     5521 gcggaggtgg tccggtattc cagatcgaag aggtcggtcc tccccgctgc gatctcccgg
     5581 agggctctct cgacggaggg gaggtcctcg ggatggatcg acctcttcca ggccagaacg
     5641 tcgctctctt cggcgcggtt ggtgccgacg atctcggcgt accggaggtt gccgacgacc
     5701 tctcccgtcg aaagatcccg gttccagatg gcgagatccg ccccctccac ggcgagcttg
     5761 agcctctctt cactctccag gagggcttcc tcagccttct tctgctctgt cacgtccacc
     5821 atgatcccgt ggatcccccg gagatgcccc tccaggatgg gcctgccgta ggtcctgacg
     5881 tgatggacgc tgccgtcttt ggcgatgaac ctcagctccg agacccggga gatcccgtcg
     5941 agggcgctct tgagcctctc ctccaggtaa cgtgagtctt cggggatgag aaattgggag
     6001 acgttccggc cctggacctc cccggcgggg tacccgaata tcctctcgac ggaggggctg
     6061 atgtaagcta tcttgccggt ggtctcgacg acgtagatca ccccgtcgac gttctccacc
     6121 aggcagcggt acctctccag ggaccttctg agagcctcgt tcgccttggc gagctcttca
     6181 ccctcttccc aagccttctc cgccgatctg tcgagctccc cgagggtggc tgatagctcc
     6241 tcaagccccc ggctgagacc ctctatcgag gggctctcct cttgaaggag ggctctcacc
     6301 tgccttcgag ccttctccaa ctcttctttc aggatatcgc gggtcacgcc gtctccaact
     6361 ccgcagaatg ttcgggccac accccgtcga tgggaggcgg ggggtccggc ccccttcaat
     6421 ggagccccga gggagaggga tcccaggagg cggcttcctc ctccatcgga gccgggtcgg
     6481 gatcttgctc agatctctca tcatgttata ccaggatgga tataacgatt tagcatcgga
     6541 tctccccgcc gccccgaccc cgatctatcg atccattaaa gcctccaccg cctctcctcg
     6601 ggaaagctta aaggagaaaa agccgaaaat ggcggccatg aagccccttt cagagctggt
     6661 gagagccctc ggagacgagg attacttcaa gaggcagagc gccgcctgga ggctggtgga
     6721 ggcggggggg gaggccgtag cgcccctcct cgcggcgctt ggaggcgacc ccgatcccca
     6781 ggtgaggttc aaggccgctt gggccctggg aaagataggc gacgagaggg gccttgagcc
     6841 cctcgcccgg gccctcctgg aggacgagga cccggcggtg agggagtggg cagcctcggc
     6901 cctggaggct atcggggacc cccgggccgt cccccccctt gcccagtctc tggcgacgga
     6961 cccgtcccgg gaggtgaggc tccgctcctc cctcgccctc ggagcctttg gcgccgtcga
     7021 ggccttcgag gatctcctcc gggaggggga cctggaggcg aggaggatgg cggtgatggg
     7081 ggtcggaagg ctccgatcta tggggtcgat ggagctcttc gcccccctct ccggggacgc
     7141 cgaccccgag ctccggagga gggtcgcctg gtccctgggg gagatggggg cctccggcgc
     7201 tcttcctctc ctcctcccag ccctgaaaga cgacggcccc gtcgtcagga tggaggcggc
     7261 gaaggccctg gcgaagatcg ggggggagga ggcctgcagg atggcggcgt ccctcctcga
     7321 cgaccccgac ccccgggtga ggctctccgc cgtcacctcc ctcggggaga tcggcctcgc
     7381 cgatgccctc gaccggctgg tggaggtcct cttcggcgag gacgaggagg agatccgggc
     7441 ctgggccgcc tggtccctgg gggagatcga cgacatccgc gcgatcgagc ccctccagga
     7501 ggcgtgcgtg aaatgccctc cgaaggtgag ggagaaggcc ctcgactccc tggaggaggt
     7561 cttcggcatg gggagggctg ccgggaagaa cggcggggga ggcgacccct gagaaggggt
     7621 ggatgcccct ccctgccccc tccccggccg atcatccccg gcccaccgtc acctattttt
     7681 cttctgcggt cccctgaagg ctgatgaata tagagtctct cgcggccgag cttcgcgggt
     7741 ttgtggggat aacccggaag aaggccatcg gcgatctcgt cggccacttt cctgtggagt
     7801 cggacctgat catagcctcc ttcggggagg acgcggcggt catcgacgac ggcgacgaag
     7861 tgatgctcct agccgccgac gggatctggt cgaggcttct ggaggccgac gccgaatggg
     7921 ccggctactg cgccgtcctc gtcaacgtcc atgacatcgc ggcgatgggg gggtggcccg
     7981 tcgccatggt ggacgtcctc tccgccggat cctccaacct cgccgaggag gtcctctccg
     8041 ggatgaggaa agggatcgag aagttcaagg tcccgatcgt ggggggtcac ctccatccgg
     8101 acacccccta cagcgccctg gacgtggcga tcctcgggaa ggcgaagaag ggggcggtga
     8161 tcctcagctc ttcggcgaaa ccaggcgact ccgtcgtcgt gggtatcgac ctcgacggga
     8221 gggtccaccc gagcttcgcc atcaactggg acagcaccag cttcaaggcg ggggagatcc
     8281 tcctccgcca gctcggatcg atgcgggaac tggcggagcg ggggctggtg acggcgggaa
     8341 aagacatcag caaccccggg atgctgggga ccctggggat gctcctggag tcgagccggg
     8401 cgggggcggt cgtcgacctc gacaaaatcc ccaagccgga ggggatggag atggtcgcct
     8461 ggcagaagat gtaccccggc atggggttcg tcgtaacgac cccggccgcg aaggccgaag
     8521 aggtcgccgg gatcttccga ggccgggggc tcacctctgc cgtcatcggg agggtgaccg
     8581 agaagcggcg cctcctcata gagtccaatg cggagaaggc cgtcctcttc gacttcgagg
     8641 ctgagacgat cacggggatc aggtgatcat gtggacgggg ggttcgtcga ccggctggag
     8701 cgggccgccc ggggccgtcg ggcgaggata gccataggcg tctccggcgg tggacccgag
     8761 ctcatcgcag ggatggagga ggcgagggat tacgccgaga tcgtcctggt gggagacccc
     8821 ggccccgaat gcgacctcgg ccattccgag ctggtccgga ccgacgatcc ggcctcagct
     8881 ctggtcgggc tcctggagga gggggaggtc gacggggcgg tgagggggaa cttatccgcc
     8941 accaaggtcg tgcggaagat cggaagaacc ttcgaggtga gggtgagacg cctctcgctc
     9001 cttgccctcc ccgactggtc cttcttcctc gccccggtgg ggatcgacga gggggactcc
     9061 atctccgacc ggctcagcct cgccgtcctc ggcgccgggt acatcagggc tatggacctc
     9121 gagaccgagc ccagggtctc gatcctctcc ggagggaggc tggaggacct ggggcggagc
     9181 gaccgggtcg acaggaccct cgccgagggg gaattggtgg cgaggctcgc ccgggacgcc
     9241 ggggtccggg cggtccaccg gggcatcctc atcgagacct gccgcggcga cgacctgatc
     9301 gtcgcccccg acgggatctc cggaaacctc gtattccgga ccctgatgct cctctcggga
     9361 gcgaggggct tcggcgcccc cgtcatcatg gacccggtct tcgtcgactc cagccgggca
     9421 aggaaaagct ttgtcgggcc gatagccctc gccagcgccc tggtggcgat gaaggggcgc
     9481 tgagtaattc gcaagaaaaa agggtcgggg tgtgatctgg ccaccccatc tatatcaaca
     9541 gatcagccgg atatgacccc gaggaccttg agggggtgga ccccggcgcc ctggagggcg
     9601 aggagctggt cgttgtttgc gaggctgaac tccttgatcg tcgagacgaa ctcctggggg
     9661 gagtcggcgg cgtccatcag caccaggtcc tccttcagcc ggtaggccca gctcggccag
     9721 atctcgatgt tgctcatgtt cccagccttg tcgaagtcga agccgaggac gaggccgtcg
     9781 ccggatttgg aggccgcatc ccatcggatg aagatcccgg cgaccctggt cccgtacttc
     9841 tccgtcaggg cagccgtctc gtcggcggtg aggtacttga cgaagaggcc gctctttccg
     9901 ggggtgcagt cccaggccac ctggaagtag tcgtctttgc accagttggg gcaggctatg
     9961 accttgtagc tctggttggc ggggtcctcg atgggaaggg tctcctccat gtacttcgcg
    10021 agcaagaagc cgctggagac cccggggcag atgtggtcgt ggagctcaac cgccttcatg
    10081 aagacgtaag gcgtattctc gtgggcccag acgttggcga tgggcatcat gtggctgaag
    10141 ttgcccggct cccaggcgga gacgttggcg aggaccgtct cggcgtcgat c
//
LOCUS       DASM01000130           18886 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_76390, whole genome shotgun sequence.
ACCESSION   DASM01000130 DASM01000000
VERSION     DASM01000130.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 18886)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18886)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18886
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_76390"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         3839 a         5663 c         5962 g         3422 t
ORIGIN      
        1 tggaggccac ctcggggaga gaggaatcgt cttcgtcctc agcggcggct ccgtccccga
       61 ggtggcctcc agggccatcc ccgaggtgat cgccgtcctg gcggagaggt gatctgatga
      121 ggatcggctc aggctgctac agctgcatcc tcgaccgggc caagttcgag gccgacctcc
      181 tcttctccga cgatgccgag aagatggagg ctatggagga gctcctcgac ttcatcgtct
      241 gtcataaaag ggaggtcccc tcggtggtgg ggaccgagcg ggagaggatc atgaagagga
      301 ggagcaaaaa ccccgacccc tatcgggaga tgaaggccga gagcaaccgg gtggcgaccg
      361 ccctcctccc cctgacggag aggttttacg accggtccga ggagaagctg gaggccctga
      421 tcaggatcgc cgccgccgcc aactccatgg agtggggggt gagggggcac gacttcgacg
      481 tcgactcctt cgaggaggtc ttcttagaca ccctcaaaga gaacctcgac ggcgatctgg
      541 aggaggcccg ccaccgcata gaccgcttca aaagggtcct ctacctcacc gacaacgccg
      601 gggaggtggt cttcgacctc ttcgtcatcg gtaagctgga agagatggga aaggccgtcg
      661 tcatcggacc caagaccgag cccatattaa acgacgtcac ctccgatgag ctgaggccga
      721 tgaccaccct ccccctggag cctacgggcc ccgtcatcgg cctctccctg gaggaggcga
      781 ggcccgagct cctcgacctc ctcatggacg agggggtcct ggtcatcgcc aaggggatgg
      841 gaaacttcga gacgatgacc gagttcgacg atatgctgga ggggaggctg atctacgtca
      901 tgagggccaa gtgcgatccc gtcgccgatg ccctgggggt accccggggg agcctggtgg
      961 tgagggcggt ctaggccgga gacgccatgg ggtcatgcat accccgccgg atcctcctca
     1021 tcccgacgac gaagcctcga ttccccgtcc tccccatcct tcggctcctc ctgacggctc
     1081 cagcccagga cctccgtcga gatctcgagc cccaagaggt cgcttgtggc cccgatcagg
     1141 ttctcggccg ccgccaggtc gacggccttc tccccatcgg tcttccccat gaggcagacg
     1201 ccccggatcc cgtagaaggg gcagaggccg acgaggatcc cgttgaggcc tccgacgatc
     1261 ccgcttcgga ggagggtgac gccgccccct tccagggcga gggcgaggtc gggatcggtg
     1321 gcgacgccgt agaccgtatc ctcggaggtt ccgacgtagg cggcgagggt cactagatcc
     1381 gtcacctcgt aggaggcgag cgccttcagg atctctcccg ccaggttgta catatggggc
     1441 ggcttcaggg gctgggcgtc cccgcagagg attatcaggt catcccgacc ctccggggcc
     1501 ttcaactcca cctggagggc cctcgccaac ccatcctcca ccgggacctc gggggggaat
     1561 ccggtggaga ccatcagctg gaaggtgatc gactccaggg cggctgctat gtaatctgcc
     1621 gccaccttac cgacgctgcc gatgccgggc atgccgccga tgcagacgca gggtccgaag
     1681 atcttctggg acatggtgga ggtcgccccg gctctatcag tgccctacag ctctgatgat
     1741 ccagttcacg ggcaccatct cctccgattc gtcgatggcg acctccgcca ggaagatctc
     1801 ccccgtctgc aagtcgtaga agtaagaatc tccctcgacc tcgtgaaggt ccactcccac
     1861 cgcgacggct cctcgatcat agaagcagac gtagaagtcg ccgttaatgg gtatgaaagg
     1921 aacttcgatg acgctgatcc ccggctgtcc ctgattggcg aagtatggta gatgggaatc
     1981 agtgaacctg tagaggaggt tcaggttctc atccctgatc tccatgctta ttatccgatc
     2041 ttcaggaagc gtaccattct caccatacgg gaccaagctc cagatctgga caccatccag
     2101 ggtccagctc ggcctcggcg gcgtgaacct gaccgcttct gcaaccttca gatatcccgg
     2161 gctgcgatag gtggctacgt tcactatggt ttcatttccg ctgtcctgga tcagaagatc
     2221 ctgcgagacc tcttgggcga tagaaggtcc aaccagagat aggaccgcaa agcaacataa
     2281 ggccgcgaga agtgaactag tcttcttcaa aggatcacca tcctcaatta gatcgccgaa
     2341 tatatagatg atgccgcaag gtcggactcc tcagatttca gcatatctcg agcttcggcg
     2401 gcgaaggggg gctggcccgg gctcaacccc aagaacgggg ctgacggcca gacctcccct
     2461 aacccctccc catcctttct cccaccatca ctattggagc ggcaaggtgc cggtgtggcg
     2521 gaggttctgc ctcaccatgg gccactcatg atctccgaag gcgccggaga tctcccagat
     2581 gtatatcctg ccgtcgtcag accccgtcac catctcgagg acgccgtcac cctccaggtc
     2641 ggcgatcgcc ggggtcgtgt cgacgttccc cagggtgacc ttgggaaagc catccacctc
     2701 tgttccgtcc agcctccagg cgtggacccg gttgtccagg gagcctccta tgatctcctg
     2761 ctggccgtcg ccatcgatat ccgccagggc gatgttcttg acataaccgc cggtctggat
     2821 ggggaagccg ggatgatcgg agccgtcgct cctccagagg tagagcctgc cgtcggtggc
     2881 tccacccacg atctcgacct ccccgtcacc gtccacgtcc cctgcggcga tgaagctgtc
     2941 ctgaagagat cctgtcgtct tccgaggaaa ccccctgtaa tcctccccca cccgggtcag
     3001 ggtgtagagg ccgtcgctgg cgccgatgac gatctcgagg ctgccgtcgc cgtccaggtc
     3061 cgcgaggatc ggagacgagt atcctatggc gctgccggag aggtcggtga gggaggggct
     3121 gctcaggtcg acggggtatc ccggcagaag ggatccccag ccgtcgacgg cgtagacctg
     3181 gccggtgtaa agtcccagga agctcgccaa gatgtccgtc cccatcttcg aggagccgat
     3241 gacgccatcc aagagaccgt cgccgtccag gtctccgatg gcaggagagg accagacgga
     3301 gttggaggtg atcagcggat agccgcagac gggggatccg tctccgttga aggcgtatat
     3361 cccccggtcc gtcgacccca ccaccacctc gaggccgccg tagccgtcca ggtctccgat
     3421 ggcgggagag gaccagacgg agttggaggt gagccgggga aagccggaga gctcctcgcc
     3481 ccggccgttc caggcgtaga gcctgccatc gtcggatccg acgacgatat ccaggagacc
     3541 gtcgccgtcc aggtcttcta cggcggggga ggagcggatg tagtcttcgg ccctcgtcgg
     3601 gaagcctgta accttcgagc catcccggtt gaagacgtag agccgcccct cattcgtccc
     3661 caccacgatc tccagctccc cgtcgccatc gatatccgcg agggctgggg aagaccagac
     3721 ggggctggag gtctggatcc attctccggt cacgccctcg tcgatgggga ggagcctgaa
     3781 ggagcctacg aagctgtcga agtagaggag gttggccctc ctgaaagccg actgctccgc
     3841 cctgcaggat atgtggtata tcaggtcttc gccgttcaga aacacgtctc tcatgatgat
     3901 ggaggtagat tcgtcctcca gaaggtactc cttctccagg gacctgacgc cgttcaggtc
     3961 tccggcagcg gacttcatca cctcaaagtt ttcgctccac tcttggtagc ccgattccgc
     4021 ccgggaggag aacctctcca gagagaggcc cctcttctct gaggccccca ccaggatcgt
     4081 catcacgccg gaggcggaga actcgacgac ggggtcctcc acccctgagg taacgtcctg
     4141 ggggaggagg ttgatatcca gctcccttgg gtactctgca gagaagccga actccaggct
     4201 ttcgaactcc tcggtctggg agctcgccgg gtcgatcagg gccgcggaga gggccaggat
     4261 caagaatgca tatccacgat tcatcatata ttcacttcca agttgctcag gttaaccagg
     4321 ccccacccgt agatctcgtc ggggccgggc ggccccaggt ccgtggagct ctccaaaagg
     4381 atcctcctga cgtccaagtc cggatcgtcc ttgaagatct ccaggaccag ggcggcgcat
     4441 cccgccacga agggggcagc ggctgaagtc cccacgaacc cctcggtcgc gtaggaggag
     4501 gtcgtcacgt tgtccgcggc caccagatcg ggtttctgac gcccgtcctc ggtggggccc
     4561 cgggagctgt aaggctggat ctccagggtg gagatgttga tcgccccgac ggtgacgacg
     4621 tttcggcagc atcccaggat accgatgctg gtatttggga cctggtgctc atcgaggtcg
     4681 gaggtggtga agaggtggag ccgaccggga tgatcggcgc cgtcgtaact ccggatcttt
     4741 atctgatagg ttccgtccct gggggcgacg aggcggatcg tctccaccgg cttttggccc
     4801 ctgtatcccc tctgggggtt gtcggagacg gaccgccccc ggccgggatg gtcgaggcgg
     4861 aggtcgtaat cgagggccgc agcctcccaa tcgtccttcc agctgagcca gatggagacg
     4921 tcatcccccc tcttcagggt gaggttgatg ctctcgtcct ccggcgagaa gttgagaaaa
     4981 ccgttgccat cgggatcttt gaactcgccg gaccagtggc tgagagcctc gtttccggcg
     5041 gcattgatcc agagggtccc gttggaggtc atcttcacga cctcctggca gacggggtcc
     5101 tcgccgtcga acctcccgaa ggggaagtcc gaggagcagg agacgatgtc gattctctcc
     5161 tccagctcca ggagcctctc caccgcccgg gctaccccct cctcggtgat ccccacgtcc
     5221 accagataga tcttgacccc cggggctatc aaggatatca gctcggcgca ggcgatcccg
     5281 tggacctcat ccttgccgta gctctcatcg tagaggggta cgatctcgga ggtgagcttt
     5341 ccggcggtct cggggtcgta gtagaactgg ctgtccagga gggcgatcgt caccccccga
     5401 cccgtgatat tccaggcgtg gagggcggag atccccgtaa tatccatcat ccgccggagg
     5461 gaatgggggt ccagaaccgg ctcagatccc tcctcgtcca tagatccttc tatgcccatc
     5521 gatccgcctt cgtccgacaa accacatcct agggaggtct tgccgtcgtc ccttgcgccg
     5581 atctgaggag agggcagagc ttcgacctcc gccggggcag ggccagttcc ggaggcgagg
     5641 aggcctccgg aaagggcccg accatctcca tcgcaaatcc cggatgtgga tggagcggcg
     5701 aggatcgcca gagctgaaag agcgatgagt ataaacgatg agagtctcat gggtgccgtc
     5761 acgtccgtct taatttccaa gcaatgataa ggtaggtatc ttatataaga atatcctcat
     5821 ggatctcctt ttgccgcgca tccttttatt ataggatccg agctgtgcct ctcaatttct
     5881 atgaaggtcg ggggggcttg gaaaaggggt cggccctccg aagatccgaa cctcggccat
     5941 cctactccaa cccggaggag aggacgaaaa aaggatctac caggcccccc atcccccacc
     6001 catgcgaagc ttcgtagtgg tgggaaacct cgcgacgacg acgccggact tctcccttga
     6061 ggatatcccc ggcacctcag gacggatcga catcctctgc cgttgcatca actcggcctt
     6121 cgtcctctcc cacgggatcc gaagggacgt ccagatctac ctgatcctcc gaggaggcgg
     6181 tgccccaaaa accgttctgc ttcaggggcg ggacctccgc cacctcaacc ccgacgagag
     6241 gacgacggcg gcgctcctga agaaagccct cgccctggag gcggactttg cctggacgaa
     6301 gtcgacgccg gggatctccg tccgggccgg agacctggcg gacctcccga tacgggccga
     6361 ggggaggacc tactatttaa gggaggacgg atctgaccat cgaggcctcc ccctggctgg
     6421 agacgggacc ttcgtcctcg gagaccacat cggcctatcg gaggaggacg aggccttcct
     6481 cgccagcctg ggggccgaga tcgtctccgt agggccgaag agcctccacg ccgaccactg
     6541 catcgtccta ttaaataacg agctggaccg gagggagggg ccgtccagcc cggccggagg
     6601 acgagagagg gcgacgaagg gggacgaata aagagaaggg acgaagaaga gacgcgtcag
     6661 catgaccacg attgataaga atgaagaggt cgccgacctc gcggaggggg cgaggaggat
     6721 cctccgcctc ggccccatct gcgacagctg ccttggccga cagttcgcga tgctctcgac
     6781 gggcttgact aacgccggga ggggggaggc gataaagacc ttcctttcca tggccggctc
     6841 catggacgaa ggaggccggg agatcctgga ggagctcgcc ccaagctcga agagcgcaag
     6901 gctgaagctt ggccttccgg gggagggcga gaggtgctgg gtctgcctcg gggagatgga
     6961 gccggcgatg ctcgaccggt gggccgaggc cgccgagagg gagcttcgcc acctcgagca
     7021 cgagaccttc ctcgtcggca ccaggatgag cggcctcctc gccgagaacg aggagctgat
     7081 cctcgcggac ggcggctccc gccacgccga gccgatgaag tcggagctga accgcgaggt
     7141 gggaaagagg atcgccgaaa ggacggggaa ggcgacgagc ttcacctccc ccgacgtcct
     7201 cgcggtcctg gacctggagg agggggcggt ggaggtccag gtctcctccc tctacatcca
     7261 ggggaggtac agaaagctcg tccggggctt tccccagacc aggtggccat gccgcgattg
     7321 cggcgggagt ggctgcgagg cttgcggctt caccggccgg aggtatcagg agtcggtgga
     7381 cgagctgata agagacccca tcatcgaggc ggccaggtgc gaggacaccg tcttccacgg
     7441 ctcggggagg gaggacatcg acgcccggat gctggggacg ggacggccct ttgtggtgga
     7501 ggcggtctcc ccccgggtga ggaccctcga tctcgcccgc ctcaaagagg agataaaccg
     7561 acgggcctcc gggaaggtgg aggtgagcga gctcgagttc accgggaagg ataccgtcga
     7621 agggctgaag aaggccctct tcgagaagag ctactcgatg gtggtggagc tcggagagga
     7681 ggtaccagaa gaaaagctta aatcagttct ggacaaactg gtcggcttgg ttgaacagcg
     7741 cacccccacc cgggtatctc accgccgggc cgaccgggtc cggaggaggg aggtcctcga
     7801 gatgaggctt gatcagctct cggggaagac cgcgaagatg accctccggg ggacgagcgg
     7861 cctttacgta aaggagctgg tcagcggaga cggcggaagg acgaagccga gcctctcggg
     7921 actcctcgag gtcgaggcga gggtggcgga gctggacgtt ataaacgtag gtggagaggc
     7981 agatgcccaa gtcacacgga atacggaaac ggactagaga taggctgagc aaatcggtca
     8041 gagagcgggg gatctccccc gtcagcaggg cgatccagga cttcgacgat ggccagatgg
     8101 tccacatcat cctcgacccg agcatccaca gggggatgcc gaaccccaag ttccacggta
     8161 agaccggcaa ggtattgggc cgaaggggga gggcttacgt cctggaagtg agggacggaa
     8221 acgccaagaa gacggtggta tcccttccgg aacatctgaa accccagaat tgagatgatc
     8281 gtcaagagca ttctgagcga ggatctgatg accctcgccg aggtgaagga ggccctggag
     8341 gagataaggc tccggagggc cgaggagccg gaggagctcg gctacgagct gagaagggcg
     8401 atcaagcacg ccgagatctt ctctaagggc gaggcggccg agagcagaaa gatggtcgag
     8461 gagctcatgg ggctggagaa gatgaccaat gagatcgcga tcaagatcgc cgacatccgg
     8521 ccaaagacca aggacgagat cagggccctc tacgccaagg agaggttcac cctcctggac
     8581 gaggacctgg acgcgatcct cgacgtggtt ctgagaggat gaccatccga ttgtgaggac
     8641 gaacaatgac ctcggagaga cccccacgac gcgaagagat agcttgggtc ttggattacc
     8701 tcccctacgg gcacgtatcg gactccatgg gcaggttcca gaagaggccc ctcatccagg
     8761 gggtggggga gagggacttc gtcctgatgg agatgacccc caaggagggg gcggtccccg
     8821 agatcggggt cagggtcttc ctggtgggaa aagaccgccc gatcgtcgaa cgggtcaacc
     8881 ggaggatcgg ctacgaggag ctctcccagg gggcaagggt ggagctaccc tacgccatcc
     8941 agaagatcgt cctggagaag gaggcgtact acctccgctt cttcaacgag gcggggccgc
     9001 tgactacgag gatgcacgcc ctggagctcc tccccggcat cgggaagaag ctgatgtggg
     9061 cgatcttgaa cgagcggaag aagggcccct tcaagagctt tgaagacctc tcggagcggg
     9121 tcaaggggat atacaacccc gagaagcttc tggcaaagcg gatcgaggac gagatcgcgg
     9181 acgatcggat caagtaccgg atcttcaccg cggatccgcg ccgacgatga gggatgcggg
     9241 gcggtgcccc aagatcctcc gatcctgctt ttaagggggg accctgcctt gaagaagctc
     9301 ggccagcatt ttctcatcga ccggcgggtc ctttccagga tcggagatta cgcctccctc
     9361 agccgcagtg acgccgtcct ggaggtcggg ccggggacgg gaaacctcac ccgcgtcctc
     9421 tcggagcggg cggggagcgt ctatgccata gaggtcgacc cggccctggc gtcgtccctg
     9481 gaggggcgtt ttgagaacgt ctcgatcatc cgaggcgacg ccctccgggt ccccctgcca
     9541 gagtacaata aaatcgtctc gaacctaccc taccagatct cctcaaagat caccttcagg
     9601 ctcctggcga ggcccttcga ccttgccgtc ctgatgtacc agcgggagtt cgccgagagg
     9661 atggtggcgc cgccggggag gagggagcac gggaggctcg ccctcaacgt cgccttctgg
     9721 gccgaggccg aggtcctgga gaccgttccc cggggggcct tctcccccag gccccaggtc
     9781 gagtctgcca tcgtccgcct ccggccccgg aagacgccgg cacccgtcga ctggggaggc
     9841 ttcgaagccc tcacccgggg cctcttctcc atgaggagga agaagatcaa aagagccctc
     9901 gccgccatga aggtcccccc cgatgccctg tcgcgcatcg accccgccct tctggagagg
     9961 aggccggagg agctctccct ggaggaggtc gtcgatatgg cggaggccat atcgggccgg
    10021 tgaggggggc cagatggggc ggaagcctcg attttttata ctcaacggcc cactctacca
    10081 ccgatgatag catctgtcga gcgctcgcag gtttcggggg aggtctcggt cccgccctcc
    10141 aagagctaca cccaccgggc gatcaccatc agcgccctgg gacccggcgg ctccgtcaag
    10201 aggccgctat tatccgccga caccagggcc accatccggg ccgccgaggc cttaggggcg
    10261 gagatcgcga tagagggtga aacgctggcg gtggccggcg ttggagggga gccgaagacc
    10321 ccggagaacg tcatcgacgt cgataactcc ggaaccaccc tccggatcat ggcggcggtg
    10381 gcggccctca ccgacggggc ggtcctcacc ggggacgcct ccatacggcg gaggcccaac
    10441 ggccccctcc tcagatccct agccgacctg ggggccgagg ccttctccat aagaaagaac
    10501 gactgcgccc ccctggtggt ccgggggagg atccggggcg ggaggaccgt cctcgacggg
    10561 agcgtcagct cccagttcct ctcagccctc ctcatatcct gccccctcgc cgaggaggac
    10621 tccgagatcg agattaaggg cgagctgaag tcccgcccct acgccgagat caccctggag
    10681 atgctggagg atgccggggc gaagatcgag accgattttc gcagcttctt cgtcgagggg
    10741 gggcagagct acgatctgga cgggtacacc gtccccggcg acttctcctc cgcctcctac
    10801 ctcctggcgg cggcggcggt gaccggctcg cgtctcaccg tccggggcct ccgcccctct
    10861 aagcaggggg actcggcgat cgtcgccatc ctccaggaga tgggggcgaa gatctcctgg
    10921 gaccggtcag agggggttct cgagatcgag gggcgcgacc tatcgggggt cgaggtggac
    10981 gcgagcctca cccccgacct cgtccccacc atcgcggtcc tgggggcggt ggctgaagga
    11041 cggaccgtcg tcagaaacgc agagcacgtc cgccacaagg agaccgacag gctcagggcg
    11101 atggcgacgg agctctccaa gatgggggcg aagatcgcag aaaagcccga cggcctggtg
    11161 atcgagggtg gagggcttcg gggagccagg gtcagcggcc atcacgacca caggatagtg
    11221 atggccctga cggtggcggg gctcgtcgcg ggagatacga tggtggagac cgccgaggcc
    11281 gtctccgtct cctaccccgg cttcttcgag gagatggcga ggatcggcgc caccgtcgaa
    11341 gtcggcggag aatgagccgg ggatcgcctc cataagtctt atgtacctgt agcgaatctc
    11401 agccttggat cagcgtttgc gattcggttc gccccatcgg ccccgcggcc gatgcgcggg
    11461 cggcgtcgga cttggggtcc ggggctgctc cagagggcct ccggattcca aatccagagg
    11521 cctcagattt ctggaggaat aatttggcag aagagaatga agagctgaaa catatagtgc
    11581 gcataatgaa caccgacctc aacggtcacc agcccgttca gatggcgctc acaggcatca
    11641 ccggcatggg aagaaggaac gcccggctcc taacccagaa ggcggagatc gaacccactg
    11701 agaccctggg gaagctctcg gacgaggcga tatccaggct gagggagatc gtcgagtcgg
    11761 cccaggagca cctcccggtc tggatgaaga acaggaggaa ggacctcctc accggagagg
    11821 accgccacgt catgggtacc gatctcgtca tggccatgag ggaagagatg gatatcatca
    11881 ggaagacccg cagctataga gggatcaggc acgagcgcgg cctccgggtc cggggccaga
    11941 agaccaggtc caccggcaga aggggagcca ccgtgggcgt cagcaggaag aagaagtgag
    12001 cgaggcgggc tattatggga tatcctggca agtgtaggaa gagttacgat actccgcgcc
    12061 acccctggga agccgatagg atggctcagg aggtggagct cgtcaagaat ttcggcctcc
    12121 ggaacaagag agaggtctgg aaggcccaga gcctgataag aaagtaccgc cgggtaagcc
    12181 gtcagcttct ggcagccacc acccggggag aggcctccgt cggatctcag gcggaggatg
    12241 tgctggagca cctcaagaaa tacgggatgc tgaaggaaga tggggatctc gacgccgtcc
    12301 tgtccataaa ggtggaggac cttctggaac ggaggcttca gacccaggtc taccgtcagg
    12361 gcctcgccaa gaccctcagg caggcccggc agttcataac ccacggccac atccagatca
    12421 cgggccggaa ggtgaccgtt cctggatact atgtcaagcg gggcgaagag atgaccatcg
    12481 actactaccc tggctctccc atggctcagg aggctcatcc tgagagggcc accagaatac
    12541 tcaaggtgga aggataaaca tggcagaagg aaaatggggc gtcgcccaca tatacgcctc
    12601 cttcaacaac acgatcatca ccataaccga cctcaccggc gccgagacga tcgccaaggt
    12661 ctcgggcggg atggtcgtca aggccgcaag agacgagagc tccccgtata ccgccatgca
    12721 gatggcaggc cagatcgcgg agcaggcgat gaccaacggc atcatcggcg tccacgtcaa
    12781 ggtcagggcc cccggcggaa accgtcagcg ttccccggga cccggagccc aggcggcgat
    12841 cagggccctg gcgagggccg ggctcaagat tggcaggatc gaggacgtga cccccatccc
    12901 ccacgacggc accaaaccct ccggcggacg gcggggcaga cgggtgtgag ctttgcaggt
    12961 tgaactgatc gagctggtag agggccgggc ccggttcctc ctcaggggcg tgactccagc
    13021 cttcgccaac gccataagga gggcctgcct tgcggaggtg ccgtccctcg ccatcgatga
    13081 gataagtatt tatgacaata catcggtcct cttcgacgag cagctctccc tgaggctggg
    13141 cctcgtgccg atcaaggccg acgacctcag cctcttccgg gtccccgaag agtgcgagtg
    13201 cggcggggcg gggtgccccg cctgccaggt ggggatgacc ctctcggcgg aggggccctg
    13261 caccgtccac tccggcgatc tgaggttctc ggatcccggg gtgaaggtcg tcttcgagaa
    13321 gatcccgatc gcgatcttgg gcgagggcga gaagctgatg ctggagggga tcgtcaccct
    13381 caaccggggg acggtccacg ccaagtggca ggccggaacc cagtgcggct acaagaacct
    13441 gccggagata acgatcagcg atcggtgtga aggctgcgga aagtgcgtcg aggtctgtcc
    13501 ccggaaggtc ctcgtcatcg gagagggcga ggggaaggtg agggtccacg accccatcag
    13561 ctgttccctc tgcaagctct gcatcggaga gtgcgatgtc ggggcgatca acgtctccgc
    13621 cgtcgaggac gtcttcgtca tgaagataga caccgacggg tcgatgcctg caaaagacct
    13681 ggtgacaggg gctgcagggg agatcaagag gcgcgcgacg tcgctcagcc agcagttgag
    13741 cgatttggct tagtgagggc gaagttaaga ccacgggcgg gggttgccaa gccaggtcaa
    13801 aggcgcaagg ttgagggcct tgtctcgcag gagttcgtgg gttcgaatcc catcccccgc
    13861 accaacttac ctcttctggg tttttcctat ccggtattgg ccgtctctgg taggcatttc
    13921 gtaacaaaaa aataatataa tattagtcga tacgtttttt gatcgataag ttaacactca
    13981 atgactgata accgcagatc cgacttcctg ccccagccct gaaatccgcg gctgatcgtt
    14041 cacctcaacc cttccgtcac catcatcgct gcaaagcgca tggggcccgg ggcgggaaga
    14101 tctacccagc ttcaaggatg ttttggggga cccgccgccc cgagagctct ctggcgagct
    14161 aaatgcgtac attgcataac tccctatcgc cggagagccg ggaaagaggt ggtggacccc
    14221 tggggcctcc tcattcccga tcggtcgccc aggggttctt ctcctggcct actatggaat
    14281 tgaatggaag agtccttctc tctaaatggt gcatcggtat tcacctgaat ttgatcagtt
    14341 accaaaagtc tttaacccca gagctccttt tagatcctaa atcaggcagg aggatctgaa
    14401 ttatgttcag ttggctctgg tttctgatca ttgggatcat cgcaggctgg ttggcaggcc
    14461 gcatcacacg agggaagggc ttcggccttg tagggaacat ggtgatgggg gtcatcggag
    14521 ccttcgttgg cggttttctc ttcgggctgg tgggcctctc tgcccacggc tttgtgggat
    14581 cgatcatagc agccaccgtc ggcgcggtgg tgttcatata cctgaccagg tacctgaaga
    14641 agatcggatg atgccgttat cgttcatggc ctggaagatc gaggaagctc caagggcgtg
    14701 gcccgatcct ctatccgatc tgaggaaaaa tgttcaacgg gggcttcttc tggagggcct
    14761 cggccgttgg ccccgccccc gacccgatgc cggcaactta ctaaaagagc ctagcaggag
    14821 caacaggctt taatactttg atggacccta aattctgggg tgttagctat gaaggctatg
    14881 atcttacttc cactgttggc cctggggctc ctctgcacca ctggagccgc agatactcca
    14941 tgctgcgggg ggtcggggga tgggaccgag tctctgtata aaaaggagcc ccctaagggc
    15001 tggttgtctc cctcgaacta taagtcgttc tccagccaag ccaactgccc ctacgattac
    15061 tgctaccata aaagatcctg cttcgcgcac tccaggtcgg cgggttatta ttatacctat
    15121 ccatacccga tgggccgcta ttacacctgg tactactttc cgtacagcta ctacaagtac
    15181 cccacggggt acttctatgg ccgggccggt tatcctttgg tcataggttg aggacccgcc
    15241 gaggaagatc tctcgccgcc atagatgacg aaggtaggct cctcccaatc cgccctcctg
    15301 gcccggggaa gggagccgtc gccccccatg cccttctgac cgtacgccaa ggattatcca
    15361 gatcaggctc ttaggcgccc cctgaacgta tctaactcca tcccgatatc ttccgatccg
    15421 cccaacttcc caaaagagac gagcaggagc aacaggcttt aatactcgga tcgcccctca
    15481 gagagttggg gtgttagtta ttaaggcaat tattatgcta cctatagtgg ccctggcgct
    15541 cctctgcgcc accgcttccg cccatcctcc gggagatgaa ggtccgagat cttctatcgc
    15601 tcctcctgat catacccctc gccacgactg gctctctccc tggaactctt attacagcta
    15661 cacctattat ccctacgact attattctta caactattat ccctacgcct actactaccc
    15721 gagcgggtac tattacacct actactaccc gagcagctac tattattcgt attctccacc
    15781 gtacagctac tactacccat actacaggta tcccaccggg tactactata gaacgaggta
    15841 ttcgttggtg atgggttaga cccgacgtcg atatcaacgt acctgggatg gggagtttgg
    15901 acccctccag gcagaaccgc tggcgtatat gaggggtcat cccatcgccc cccaaccctc
    15961 ctcttaagag agaagagatg gtcgatccgg tgacgacccc tgagccagac ccgtgaaaag
    16021 atgaagagaa gacttttcta tggtggcgag gatacctcct taagatacgg acttgaggtg
    16081 aatggatttt gaagatcgtc ggcataaatg cgagccctcg gggctccaag agccagacct
    16141 tgaggctcgt cagggccgtc ctggacggag cccgagaggg aggggcggag acggagctgg
    16201 tcgacctctg caagctcgat atcaagtact gcaacgcctg tgccacctgc tttgctacag
    16261 gcagatgcgt atacgacgac gatttcgacg aactatacca gaagatcctg gggtgcgacg
    16321 ggctggtcct cggatcgccc aactacttcc agaccgtcac cgcccagatg aagacactgg
    16381 tggacaggat ggccgatgcc gtccactgtc agctcctcac cggcaagtac ggctgctcgg
    16441 tggccaccgc ggggagcccc accgcgggag aggtgacagg ctacctgaac aggctcctga
    16501 tcagcttcgg ggccaacgtc gtcggggagg cgggagcctc accggggatc ccgggggaga
    16561 tggaggccgc ggaggagagg gccttcgctc tcggaggggc tctcgtcgag gcgatggggg
    16621 agaagaaggt ctatcccgag caagaggaag tccaccggga gatgagagaa cacttccgca
    16681 gactggtgga gatgaataaa gaggtctgga cccacgagca cgagcactgg aagaggatcg
    16741 gttgatgggc agaccctcca ggatcgctgc cgataccacc aatcttttta tttcatgata
    16801 gggacgagca aaaacggcga tcttgtaggt taaaggggga tcaacggatg gatagaggat
    16861 ccttccggag atgaaaaaga ggagaaattg aaggcccggg gccggaaaat atccgggcct
    16921 gatcctcaag ggctcagtgg gcctctccta ctaggtcctt cccctcagcc ttcctctcga
    16981 tcgtctcgac cttgcagacg tcctcatcgc agacgatcac atcctgctcc tggaactgac
    17041 tataagagtt ttgggggatt gtgatatcca ggcccatggc cgcggtcatc agctctacca
    17101 cgtcgacaac ctttatgtca gacttctccg gcatcgctgc cctaccgtcc atgatgtttc
    17161 gcctgcagaa gggacaggtg ctggagatta tatctgcggc tatcaactgg gcatcgccca
    17221 tcctcgcctt ggcgcagtcg agggcgagat cggggatccc tgcctttacg ccgccgccgg
    17281 cgccgcagca cctctggaac tccctggaac gggccatgtc ctcgaactga accccgggga
    17341 tggacttcag gatgtccctg ggggcctcat aggcccagtc tcggccgttg atgtgcatca
    17401 gatgcctacc gttgtggcag gggtcgtggt aggtgacctt aaagttgagg ggcctcttcc
    17461 actccacctc gccgctcctc accttctccc tcagatagac ggagaggggg acgttcttgt
    17521 aggggatgta gccctcgtac cacctaggcc agtcgacggt ggcggtcctg agacagccgg
    17581 cgcaggcgta gaggacggtt ttgacccctc tatccttcag ggcgtcgacg ttatggaccg
    17641 cgtggtcctt catcaccttc cgctgacccg tcctcaccag ggccgaagcg cagcaccact
    17701 cgtcctttcc gagcatgcag aagggtatct ccagggcatt catcagccgg acggaggcga
    17761 cggcgagggc ctgctgccga taggcagcgg tgcagccggc gaagtaggcg atctccgcgg
    17821 aatccaggac ggtggcgtca ctgggaaccc agcagagcct ctcctcctgc tttgcctgga
    17881 aggggttcct gtcctttccg aggaggccgg ggaagaagga ctgcttcccg taggggccgt
    17941 tcccccgagc gacgaggttt ggcctcatcg cctcccagag ctcgacggtg ttgatcccgg
    18001 actcgcagac ggtgccgcag accccacagg tggtgcagta gtaggtatct tccgtgaacc
    18061 tgttgatctc atcctcgttg aggggcttgg ggccgaagag cttcgcccgg aggccgtgct
    18121 ggcttcccag gagctccctg aacttggcga tcttgtacat gggggtgatc tcggggcggt
    18181 cctgcctcac gtcgaaggtc ggacaccact tcatgcactc ctggcatcgg gtgcatccgt
    18241 ccagctccat cagctgggta gacatgagga atcctgatac gaggggcttc ttctcgtttc
    18301 ctgcttctgc catcttactc gcctcctctg tttgctagaa tcgtcatcgg tgcagctatt
    18361 atgtgcatgt acttgctcca gggaaggatc actacgaaga gggccaaggc caggatcgta
    18421 tggagcaggg cgaagtgggg agcgtatatc gggtacgaaa atacatctcc gacgaggaag
    18481 gcgttccccc gcatccattc agcatagaag ccggtgatgg tgatcagaaa tactccgcca
    18541 aggaggacca cgtcgtaggc cgaggtcctg gaccgcacct ctcggaggaa gatcctcctc
    18601 gcgaccccta tcgtcgcccc gaagaggagg aggtagccga agatgtcgaa ggggagagcc
    18661 atcatttcct tcaccatctc agcctcgtgg gcgagcccca agaggttgaa gtgctcaatt
    18721 atgtccataa agagggcgaa gccagacatg gcggcgaggg cgacaaaacc gtagaatatg
    18781 gtcatgtgca tcacccatct gatggggctc ctcctcaggg tccgcctgag aaggaggacg
    18841 tcgaggatga gggttttgac aaatacccag acgccgtctt tgaagg
//
LOCUS       DASM01000131            3606 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_147073, whole genome shotgun sequence.
ACCESSION   DASM01000131 DASM01000000
VERSION     DASM01000131.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_147073"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          674 a         1095 c         1251 g          584 t
ORIGIN      
        1 ctacgacctc atcgtcctca actacgccaa ctccgacatg gtaggccaca ccgggatctt
       61 cgaggcggcg gtggaggcgg tggaggcggt cgatgagtgc gtcggacggg tcgtctcgga
      121 ggtcctcgcc cggggcggag ccgtcctcct gacgtcggac cacgggaacg ccgagcagat
      181 ggaggacaga aagaccggcc agcaccacac cgcccacacc accaaccgcg tccccttcgg
      241 cctcatcgga ggcaaaaggc cgaggctccg ggacgacggg atcctcgccg acgtcgcccc
      301 caccgtcctc gatcttctcg gcctccccgc ccccgccgcc atgaccggga ggtctctgat
      361 cgccagagat taagcccaaa aggtttcagt ctctccctga tatggattcg tgctggaaac
      421 ggttccggac ctaccttcag gtcagccagg ccttcaagat agcaagaagg ctcttcgtca
      481 tgaacgcctt cgacgggatc ctcaccctga tgggggtcgt catcggcgcc cacttctcgg
      541 ggctctcaaa ccccgaggtg gtcatcaagg cggggattgg ggggatcata gctctcggga
      601 tctcggggat gagcagcgcc tacctggcgg agagggccga gaggaggagg gacctgagga
      661 agctcgagag cgccatgctc aaagacctga agaggagcca gttcgccagg gcctcggagt
      721 tcgccaccgt cgtcgtcgcc ctggtgaacg ggatcagccc cgccctgggg gggatgatcc
      781 tggtcatccc ctacttcttc gtcccggcga tcgatatgga ggctgccttc cgggcgtccc
      841 tcgccctcgg cctcctctgc ctcttcgtcc tggggatatt tctcgccagg atcagcagcg
      901 agcggatgat cgtctcgggg gtgaggatga tcctggtggg gcttctgacc atcgccatcg
      961 taagcctcgt atcggcgccg ggggctatct agggggatcc ccttgtacgg cgggatgggt
     1021 tttgagaccg acaccttcct cgagatcacc ctccagcttc cggagtggca cgaggtcgac
     1081 ctcggcccga ggaggctgga tctgatcatc cgggaggggg ggatcctgga ctggcatatc
     1141 aggaacctcc agcgggggag gtacgggccg aggcgggccg aatacctgga ggaggtcctc
     1201 ctcccccggc tcgaggagaa gtgctctcgc cttctggagg gggtcgcccc ggaatggcgg
     1261 gcgctccggc gaggggaggt cggagaagcc tttaataggg cccgggccag gataaaaggc
     1321 ttgagattca tggagcttta ccgatgcctc gaggcgggga cccccgagat gctggaggac
     1381 ctcagggacc tggtgggctt tgagaccgtc acccctccgg gaagagagta ccccgagatc
     1441 gtcgaccacc tgatccccat cttcagggac ctcggctttg aggcggagaa agtggtggtt
     1501 cccccggagg tcctcgcggg acggggggag gaggcgattg gcgagcggtc gaacctctgg
     1561 gcccggctgg acgtcggggc ggaggagacc atggtgatct acacccacct cgacgtcgtc
     1621 cccgccgggg aggggtggtc nntacggccg gggggtcgcc gactccaagg gggcggtggc
     1681 cgccctcatc gccgccctcc gggccgtcga agccaccggc cgcccccagc gaaacctcct
     1741 cgtcctcctc accaccgacg aggagggggg agggtactcc ggcctctgct actttgccga
     1801 cgaggggctg gtgaaaggag acgtcatgct ctgcatggac agcttctccg acgacgtcat
     1861 catcgggtcg aacgggatca tcacctggga ggcgacggtc cgggggaagg ccgcccactc
     1921 cggggcgagc ttcctcgggg agaacgccat cgagaaggcc cttccggtga ttgaggccct
     1981 cctcgacctg aaggggaggg tcgaggcgag gaggtccacc ttgcgggcca gctcggagct
     2041 ggaggaccac ggggttgatt tcgtccggcc gatgctgaac gtcaccgtca tccgcggcgg
     2101 gatcaaggag aacatcgtcc ccgaccggtg cgtcctccgg ggcgaccggc gggtgatacc
     2161 cgaagagagg atggaggagg cggccgcgga gctggagagg gagatggcgg cccttcccgt
     2221 cgacctggaa tggcggtcgt ggatggggta cccccccatg aggatcgacc ccgaccaccc
     2281 ctgggtcgct gaggtgagga gagctctgga gggggcctcg gggaggagcc cccgcctcgc
     2341 aggagcccag tacagcctcg accaggccta cgtggcggag gtgacgggga tccccacctg
     2401 tgtctacggc gtcggccgcc agaccgactc aaacccccac ggacccgacg agaacgtcac
     2461 cctggaggac ctctcgacct actcccggtt cctggccgcc ctcatctcgg ggtggggagc
     2521 tggggaggag aaggagggtg gggtggatga ggagcggaga gagaagaagg agggggcgga
     2581 ttgaaggtca tctcggtggt gggcaccaag aagtccggca agaccaccct ggtggaggcg
     2641 atggtccggg ccctccgggg gaggggccgg gtcgggacga taaagaacat gcccggccac
     2701 cccgtagagg acagggggga caccagacgc cacttggagg cgggggccga cgtcgtcgtc
     2761 ggcgtcggaa gcgaccgcat cctcttcaag gtctcgagag ggggagggct aaaggaggcc
     2821 ctggaggagc tgcgggcgag cggggtggag ttcgccatcg tcgaggggtt caagacctcc
     2881 ggccttccca agatcgtcct cggcgggatc gaggtcgata acctcctccg aagggtcgac
     2941 gcatcccccc ccttcgacga ggggttgata gaggagctga tcggtctatt gatcctgcag
     3001 cccgagagct aatcgacccg aaatcgggga ggagcggctt cgcaagaact cgatttattt
     3061 attttttgcc gggagaaaat ctcgacacgt ttttaaacga ggacgccttc aacgggaggt
     3121 cggggaggtt caatttgagt cgtctatgca gcgccgtcat catctcgatc ctggttctgg
     3181 tggggtcagc ggcggctgcg aactacgtct acgagcgctc ggtgatccag ggggtgggat
     3241 accggagcca cgagctgata ggccagaccg gccagggtta cagcggctcg aagatcatcg
     3301 aacggatctc cggatccggg aacttctacg atcggaccga gtttgagctg agcgtccacc
     3361 acaacgccat aaacttcacc caggaggcga ccttcgagta cttcccggtc agctaccaga
     3421 caggaaccta cgaccggaag tggatcgacc ggctctgcgt catgaactac gacgccggtg
     3481 ccgtcgtcac cgagcagtac acccacgccg agagcctgat gaagagcacc cagatcagca
     3541 ccgtcggaaa cagccccctc aacgaggtga acaacagcgg catcgaggcg agcttcaacg
     3601 ccaacg
//
LOCUS       DASM01000132           29281 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_45446, whole genome shotgun sequence.
ACCESSION   DASM01000132 DASM01000000
VERSION     DASM01000132.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 29281)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 29281)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..29281
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_45446"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         5686 a         8839 c         8937 g         5817 t
ORIGIN      
        1 tcgccttcga gtcggtggtc aagctcgttg ccttcctcgc cgcggggatc tacataacct
       61 acggcctcct cggcggctac ggcgagatcg tcggcggtat cgccgagagc gtcgattatt
      121 ctcacctcct ggatgtggat tacgtctcct ggatcactct tttggctatg tcgtcctttg
      181 ccatcctctt cctccccagg cagtttcacg tcatggtggt ggagaactcc gacaaagggc
      241 acctgaagaa ggcgatgtgg ctctttcccc tctacctcct gctgataaac ctcttcgtcc
      301 cggcggtcgc ctggggcggc ctcctcctgg gggcgccggg gaaggcggac atgttcatgg
      361 tctccatccc catcagccag gggcaggatc ttctcgccct cctggtcttc atcggcggca
      421 cctccgccgc cactgccatg gtcctcgtcg aggcggtggc ggtggggacg atgatcttaa
      481 acgacctcga gatgccctac atcgtcagaa agatcgggaa gggtcggaac ctcccttccc
      541 tcatcctctc tctgaagcgg ctgaacatcc tcctggtggt ggggctcggg tacctatact
      601 ccctcctggt gggatttcct gccctcgtcg acatcggaac cctctccttc ctcgccgcct
      661 gccagctggc gcccgcagcc ctcggcggcc tctactggaa gcgcggctgc aggcaggggg
      721 cgatagcggg cctcgttgcc ggcttcctca tatgggggta caccgccctt cttcccactc
      781 tcgccgaagc cggctggatc tccagggctc tgatcgatgc cggcccctta gggatcgccc
      841 tcctccggcc gacggccctc ctggggctgg agctggagat ctggtcccac gccttcttct
      901 ggtcgatcct ggcgaacttc accctctacg tcctcttctc cctaatccac gagccgagcg
      961 gggaggagaa ggccctcgcc gagagcttcg tcgacgtcta cagggctccc ccccgggtcg
     1021 agccctctcc cctcctcccc ttcggcgaga gcgtggtgat aagggtcggg acggtcgacg
     1081 agctggaggg gacggtggcc cggtacatcg gggccgagag ggcgaagaac gtcgtcgagc
     1141 tgaacctcgc cgacctcaag acgacccggg aggggatcga tgcgaggcag ctcttcgagc
     1201 tgaaggacag gctggagaag accctcaccg gctccctggg gccgacggcc acgaggatga
     1261 tcgtcgagga cgaggtgggg gtgaagccgg tggtggaggt gatccaggag acgagccccg
     1321 tatacaccct cgatccgggg aaggtctaca taatcccgga gaaggcctac gaggtcttca
     1381 ccgaccagat cacccacggg gtcgaagggc tctgcataac ctccacgaac cccgaggaga
     1441 tccgctcgag gtggcacttc accgagaccc ccatcatcag gctcagccac cagcgggggc
     1501 ggggggatag gtacatagcc cccaacaacc tccccctcat cttcataacg ataaagtcct
     1561 tcgtcgagaa gagcaagaac agcatcatcc tcctggacag cctcgaagag ctgatcgagg
     1621 agaacgcctc caccgtcccc gaggcggagg tcctcgactt cgtccacgcc ctggaggagc
     1681 tctccagcag gggcaggacg aggctcatca tccaggcgag gcaggagttc gtcaacagaa
     1741 ggctgtctgc ggagatcaac gaggcccatc agctgatctt catcctgggc cccctctccg
     1801 cctacctctt caaggccttc gccgactcct tcctcaagag gctcgacgag ccggccaggg
     1861 ggacggtcca gaggagcatc ggggagatgg tggagacgga gaaggtcttc gccgggatcc
     1921 gcggggaggg agagggagcc ggctgcgacc ccgagatggg cgagatcgat ctctcctcct
     1981 gggggtcttc tctgaaggta gattccaggt cgaggttcag ccgctgggag ttctttgcgg
     2041 ccctgaggcg ccttgggggg gcgatcgagg cggctgaccc gaccttcgac ctcctccagg
     2101 ccgcaaggcc cctgatggcc aagtacggcc tccaccccat agagctccgc ctcgcccccg
     2161 ggacgaccta cgtcctcgag gaggagaagc cggtccagag cctgaggatc tacgccgacc
     2221 tcgtcgcccg gggggtggag gggctctgca tcagcaggta ccaccccgag aggctgatgg
     2281 agaggtacgg cctccccccc gagagggtga tatggctgac ccagtcggcg gggatggggc
     2341 cggaggtgcg gcaggtagac cccaccaact tcccgaggct cagcgggatg atctccgact
     2401 tcctctccag ggccgatgag cccctgatcc tgctggaggg gctcggctac ctgataaccc
     2461 agagcaacta cgagtcggtc ctgaggttcg tccagtccca aagggacgag gtcgccctga
     2521 agagggggat attgatgatt cacatcgacc ccctggccct ggacctcaag gagaggcaca
     2581 ggctcgtcag cgagatggag gtcctcctgg tcggagagga aaagggatga atagggttat
     2641 ggcatggggt cgttcatggt ccaagtcgag atcctgatcc tgggattgtt cctcatattc
     2701 gcgatatacg cctctttcag cctgatcatg aggtccgtca agttcctggc cgtccacacc
     2761 ctcgtcggcc tcctcatcct ctacctcgct aacgccgtcg gaggcctcgc catcccctac
     2821 agcctgccgg tccttctcgt ctgcgccgtc ctgggggcac cgggggcgat cgccgtcatc
     2881 atcctggact tcttcggcct cggcctttga atcctagcca gcctcgggaa aaatgatggc
     2941 gctgaggtgg ccgagagccc ctccggctgt ctccatgccc cccttcctcc tcctcttcct
     3001 cctgcacctt tcaggagggc ccggccttgc tgtgcctcca ccttttgggg cccgtcctct
     3061 cctccgggtc tatcgatcct ggccggtgca gctcttcgat atgatcgttc cggacctgtg
     3121 gaagtattga acgacgaagg ccgcgaagag gacggcgatg attccggtga nntgaggaag
     3181 atgccgtcga agacccccgc cccgcctatg gataagacca ggggccttgg ggccatgccc
     3241 atcgagccgg ggccgaccat cggcggggcg agggccgcca ggaccccggg ggtgaggagg
     3301 ttgaagatgt ccgctccgag gagggtcccc atcgtcccgc tgatgtaggc gagggcgggg
     3361 gcggcctgga gcctcctcgc cagaacgagg ccagagacgg cggcgagggt gggggcgacg
     3421 tagacgggga ggaggatccc ttcgccggcg acaggcatcg ccatccagta cgttatagtc
     3481 gccacgatca gagtccccag gatcgccctc ctcgccgaga cctggcaccg gacgaggata
     3541 gtcagggata cgaggatggg gatgaggcac ccgccgaggt tcaccgccag gatggtcgga
     3601 tggaaggtgg tggggaagga gaggtccagg gctggcgata ggatcgacgc cgcctcgagg
     3661 aatacggggg ggtactcggt gatcgtcccc aaatggaggg ggaaatctac ggagcttccg
     3721 aggaagcttc cgaagaccat caagagggcg tgccagtagc ggaaccccac ctggtagaag
     3781 gcctcggccg agaggcggaa gaagaggtat acgactatga ggggggtggc gaggactagt
     3841 agcaggatcg caagggtgat ggggatcgcg agccattcga acaacttctg caccagccag
     3901 actatttgtt ccgcagatcc gttccgcaga tctgagccgc cttatcgtcc ctctgaactg
     3961 ttggggcggt taatataacc ttcccgatgg actgggccgg cggggaccga aaagaggatc
     4021 tcttttggcg agggctgaaa gacggaggtc attgatttgt ccataattgc gggaatagag
     4081 ctgagggatc tcctggaccg aggcgcagtc accgatatgg tcgaccccga ggtgcaggtc
     4141 caggtctgcg gcgtcgacct gaccttgcgg tccgtcgagc tcttcgcgac gccgggggcg
     4201 gtcgactacg acaatcggga gagaaaggtc tccaagaccg aggagatccc cttcgacgga
     4261 gccgggtggg tccacctcga ccccggagct tatctcgtca ccttcaacga ggcggtctcc
     4321 ataccgaagg atatcgccgc cctggcaagg ccccggtcga gcctcctgcg gagcggggcg
     4381 acggtcgaga ccgccctctg ggaccccggc taccggggcc ggagccagtc cctcctggtc
     4441 gtcttcaacc ccggaggcct ccggctgaag aggaacgccc ggctgatcca gctcgccttc
     4501 taccggctca gcgccaccgc ggagaagggg tacgaggggg cctaccagaa cgagaacctc
     4561 tgatcccctc tgatctgatc cgccctgata tgatctgatc tagtaagatg ctcagtatct
     4621 gcgatcatct gcgattccct cctcttcgaa gaggaggcgg cgggaccggg ggcggagatc
     4681 cgggccgagg tggtagacga ggggtactcc cgtcgggatc tcgacggcgg cgatcccgtc
     4741 gtcgctgatc ccctccaggt atttgaccag ggcgcggagg gcgtttccgt gggagacgac
     4801 gaggaccctc cggccggacc tcacctcggg cgcgatccta tcggtccaga aggggacgac
     4861 ccggtcgctg acgtcggcca gggcctcagc ggaaggaagc cgatcagccg ccaagaagcg
     4921 gtagcgccgg tcgaaccggg ggtggcgggg atcgtcgtaa tccaggggcg gcggcctctc
     4981 caaaaaaccg cgccgccaga gccggacctg ctcctctcct gccagggccg ccacctcgtc
     5041 tctgctccgg ccctggagcg ccccgtagtg ccgctcgttc agctccaccg ccttgacgac
     5101 cgaaacccac tccagctcca tctcctcgga gacgagccag agggtcttga tcgtcctctt
     5161 gaggagcgag gtgaaggcga tctcgtactc cagcccctgc gagcggagaa gccggccggc
     5221 cctccgcgcc tccgcctcgc ccgtgggcga gagggggacg tcggcccagc cggtgaacct
     5281 ccgctcccgg ttgtagaggg actggccgtg gcggaggagg atgaggttct catctgatct
     5341 caatcgaagg cccccgaaat ctcgatctcg ggccgaaggg agggatcggt ccgattcagg
     5401 agggccgttg gcggggcaga agaaatcctt ttccgacctc gcgccgaaaa aaagagaaaa
     5461 aaaggttggt gatccggcag gccctcagag gacggcccgg gccgtcgtta tcacccggtc
     5521 gtttctatct acaaccagga cctcctcgcc gtcggatata tccaggtcgg cggagacgat
     5581 ctggcggcca tagacccggc cgccccgggc gacgaagggg acggcggcgt cggatatcac
     5641 catcttcttc cttctcggag ttgcgttctc tggcacgagg tatcacctct ggatataatg
     5701 gtggagctga tcggatatga attttacggt taaaagtatg attagatctt aactttgtcc
     5761 aagatatcag ataatatcat cacgcttcta aatgactaaa cctccgcccg ccaccccacg
     5821 tcatacctcg ccccgcagtt cgggcagatc aggactatct tgtagtcggc accgtcgcgg
     5881 aggaaggaga cgtgaaaccc cctctcgtac ccgcaggcgg cgcagacccg aaactcctcc
     5941 cccgcctcgg cccgccttat ctcctcttct accttatcgc tcaagatccc accacggcga
     6001 agttgagcct caaagtattt ctctcttctt tcggcggtgc ggaggagggg ccgggccggc
     6061 cgagaggacg aatcatctta atatactccg ccctcttttt taagatcatg atcgcggcct
     6121 gcatccagct tcagatccag ctctgcgaga gagaagagaa cctccgccgc gccctggccc
     6181 tggccgccga ggcaatcgag gggggcgcgg agatcctcgt cttcccggag ctcttcctca
     6241 ccggcttctg ctaccgacgg gatttatccg accggccgcc ctatccctcc cttgaccccc
     6301 tccggctcct ggcggaggag gacggctgcc tcgtggtggg gtctctggtg gatggaaggc
     6361 tgaaccgggg cttctgcctc gacggggctg ggatcggatt tcaggagaag atccacccct
     6421 tcgggaccga gaaggagcac ttcgacggcg gcgaatcgat atcgccggtg aagacctcca
     6481 gggggaggat cggcctatcc atctgctacg acctccgctt cccggaggtg gcaaggagcc
     6541 tcgccctcgc gggcgccgac ctcctcgtca ccatcgccca gttcccgctt tcccgggggc
     6601 gcgtctggga gaccctctgc cgggctcggg ccatcgagaa ccagatccac cacataggct
     6661 gcaacggcgc gggcccccag ttttccggcg gctccgtcat cctcgacccc cggggccggg
     6721 ccttggcgga ggcggggccc gacgaggagg tgatcctcgc cgagatcgac ctcgccgaga
     6781 gggacagtgc ccggagggag atcccggccc tggcggaccg gcggcccgaa atctacagat
     6841 cctttgaggg atgaggggat ggcctgggag ggttggagga tgatcggccg actggcagga
     6901 gagatgagat cgatggaggg gcatctttga agccggagac ggtgaagttt ctccgctcgc
     6961 ggttccggga gtactacctg gaggcgtccc tggagatccc tccctcgatg gagcggcggg
     7021 agtggggctt catcctcttc gacgacatgc cgaagttcgt gatgcgccgc cacaaggcct
     7081 tctccacccg ctccgagctc gtcgactaca tccgaaccca ggtcccggcc cacgtctacc
     7141 actccgcggc gatctacgag cggccggggg cccccaccat gcgggagaag gggtggctcg
     7201 gcgccgacct gatcttcgac ctcgacgccg accacctccg gaacgctccc tcgtcctacc
     7261 acgagatgct cgacttcgtc aaggccgaga ccacgaagct cctggagttc ctgacgggcg
     7321 acttcggctt tggggccgag gagatccagg tcgtcttctc cggcgggagg ggctaccaca
     7381 tccacgtccg ggacccccgg gtcaccgatc taggcagtgg cgagaggcgg gagatcgtcg
     7441 actacctgac agggcggggc ctcgaggtcg gaaggttcat ctcggcaggg actgccgaag
     7501 gggacttcgg catcgagacc gccgacgtca tccggtgctc ccccgccggg tccccggggt
     7561 ggggcaagag ggtgaaccgg tcgatggtcg ccttcgtcga ccagatccgg tcgatggagg
     7621 agggggaggc ggtgaaggtc ctctcctcca tcgatggggt ggggagacgg aacgcctcca
     7681 agttccgcca gagcctgatg agggacgacg tcctggagcg gataagggcg ggggagagcc
     7741 tcgacctatt caaggggagc tcccaggtct ggaagcctat tatagagggt tacctggagg
     7801 aggtgggggt ccacctcggc ttctcccggg acctggagag cggcgagacc gacgagccgg
     7861 tgaccaccga cgtcaaaagg ttgataaggt tccccctcag cctccacggg gggacggggc
     7921 tccgggtgac ccccctcacc atctcgggcc tggcgtcctt cgaccccctc cgcgacgccg
     7981 tcgtcttcgg cgaccggccc gtggaggtct gcctccgggg gccctacgcc atcgagatga
     8041 agggcgagag cttcgacctg gaggaggggc cgacggtcct cccccagtac gcggccgtct
     8101 tcctcgcggc ccggaaggtg gcggagctgg ggaaagcttt agaccgatga gaggggctaa
     8161 tatccctctc cgacgtgggc gttgaggacg gccgaagatg agcgacctgg acctggtgaa
     8221 cctcctgcga aaggaacgga aggcggcaga gcttctgccc ctggagcccg gcttctacga
     8281 gaaggtgggc gagtacctcg ccgagctgga gcgagatcgt tcccaggtga aggaccccta
     8341 ctccgttgag gcccagatcc tggaggacga attgaaggcg gcccggggtt ccatcgggag
     8401 gctgatcgac ctccggatca agaagatctc aaagaaggtc cagcgggcgg gctccgcctc
     8461 caaggacgtc gccgtcaagg ggatgacccc cgaggaggag cggatttaca gggataccct
     8521 ggccgccctc accgaggggc gggagtcgat cctcgcccgg gtgaaccggg ccgagggccc
     8581 gaccaccgaa agggctttaa cgcccaagag gggtatgaac caggaatacg cccttgtgat
     8641 gatgatggag agcgttccga cgttcgttgg cgtcgatggg aggcgctact ccctagaacg
     8701 gggagatctg gtgatgctcc cggcactgca cgccaagaac ctatgcaata aaaatttagc
     8761 tcgcgaatgc aagcaggtgt gaacccatga agatgccaaa agagtttaaa gctcattgtc
     8821 ctttctgcaa gaagcacacc tcccacgtgg tggagagggt gcgaaaggga cgggccacct
     8881 cgatggccca catcgaccga cagaagcagc gggcccaggg tataggaaac tctgggaagt
     8941 tctccaaggt ccccggcggg gacaagccga cgaagagggt gaacatcaga tatcggtgca
     9001 gcctgtgcaa gaaggcccac aaccgcaagg gcttcaaggt ctcaaagttc gatctggtgg
     9061 agggttaaat ctgtcgaaat ttttgaaggt caagtgcaac gactgcgaga acgagcaggt
     9121 catcttcggc aacgccgcca ccgtcgtcaa gtgcctcgtc tgcggcagga ccctggcgga
     9181 gccgatgggc ggcaaggccc agatcaagac ccagatcctc gaagtgctag agtgatcgat
     9241 atgcagcgtg agggttggcc tgaacctggg gagctggtgg tctgcaccgt cgaccaggtg
     9301 gtggacttcg gtgccttcgt catcctggac gagtacgcga acaagagagg tctgatccac
     9361 atatcggagg tggccagcgg ctggataaag tacatccgcg accacgtccg cgaaggccag
     9421 aagatcgtct gtaaggtcct gaacgtcgac ccctcccggc accacatcga cctctccctc
     9481 aaagacgtca acgagcacca gaggcgggac aagatccagg cgtggaaggc ggatcagaag
     9541 gcctggaagt ggctggagat ggcctacgag ggagacgagg agcacctcgg cgaggtgacc
     9601 ggagccctcg tcaaagggta cgagagcctc tacgtcgccc tggaggacgc tgccgtccgg
     9661 ggcatcgacg ccctcgctga ggcggggctc cgccccgagg acgcggagaa agttctcacc
     9721 atatctcagg agaacgtcaa gatcccctac gtcgagatca ccggctacgt cgacatcacc
     9781 agccccgccc ccgacggggt ggagcagatc aggaaggccc tcaagaaggc ggcgaagacg
     9841 aaggaggagg acgtcaccct ggaggtgagg tacgtcggag ccccccggta caggatccac
     9901 gtcaccgccc ccgactataa gctcgccgag gcggagctgc ggaaggcggc tcaggcggcg
     9961 ataaagttca tagagaagca gggcggcgaa ggcgccttcc acaggcaggc atgaggtcga
    10021 agatcaagag gtgcaggacc tgtggcctgt acaccctgaa ggatatctgc cccatctgtg
    10081 gcgcatccac cagccagacg aagcccgccc gtttctcccc cgaggaccgg tacggtcggt
    10141 accggcgggc cctgcgccag gaggccgaat gagagaaacc accgttcgca gattaatgga
    10201 cctggagctg aaggacccca tcctgataga ggggcttccg ggcgtcggcc acgtcggaaa
    10261 gctcgtcgct gagcacctca tcgaggagct ggaggcggag cttgtcatcg aagtctactc
    10321 cccccacctc cccccccagg tcatcgtcct ggacgacggg acggtgaagc aggtcagaaa
    10381 cgagatctac gtccaccgcg gcgctgaccg ggacctcctc ctcctggtgg gcgactacca
    10441 gagcgccacc aacgaaggcc actacgagct cgcgggcgcc ttcctagacc tctgtgagga
    10501 gttcggggtc aagaggatct acaccctggg cgggtacggc accggccagt tcgtcgacaa
    10561 gccccaggtc ctgggggccg ccaacagcct cgagctggtg gaggagatga agagctacgg
    10621 cgtcgtcttc caggagaacg agccgggcgg ggggatcgtc ggggtcagcg gccttctggt
    10681 gggcctatca aggctcaggg atatcgacgc cgtctgcctg atgggcacca ccagcggcta
    10741 cctggtcgat cccaagagcg cccaggaggt cctccgggtt ctgtcgaagg ccctggagat
    10801 cgaggtggac atgcaggccc tggaggagcg ggcgcgggag atggagcgga tagtaggcaa
    10861 gctccgggag atggagaagg cccagatgcc cttcgagacc agcggcgagg aagacctccg
    10921 ctacatcggt tgatctcatc atgatcgagg atctcttcga gggagcccgt caactcctac
    10981 ttttggccga gaaggcgggg gcggaggagg cggaggtctt cggccttatg gtcagatccg
    11041 tagacgtcga cctccggagg gatcgggtgg agatggcgag cgagagcctc tcccggggcc
    11101 tcggcctcag ggccgtcctg aggggggcgg taggattctc gtccaccagc gacctatcga
    11161 agctcgatat ggtggccgag ggggcagttc gggcggcccg ggcccggggc tccgacgaaa
    11221 agtggcggtc attgccggtc ccgagggcgg cggcccgtcc cgagggggtc tacgatccgg
    11281 cgacggccga tctgggtcct gaggagtgcc tggagctggc ggctaccctc atccagggcg
    11341 cggccacggt cgccggggcg gagcccgtct ccgggggagt ctcggccgtc tcggggacgg
    11401 agcttctgat caacaccaac ggcctggagc tctgggagag gggtacgacc ttcaccgcct
    11461 tcatcgagtc ggtggcgagg gacgccgacg gctccgcctc gacgggctac gattttgcca
    11521 actccaggtc cgcccctcaa gacctctcgg aggtggggag gtccgcgtcc gacctcgcgg
    11581 cgaggtctct gggcggggtc cggggggagg acggggtcgc ccaggtcctc ctggggccca
    11641 tagccttcgc cgagatcctc gacaacgcct tcctcccctc caccttcgca gaccacgtcc
    11701 agaaggggag gtcgtccctg gcggggagga tcggggagag ggtggccgag gagagcttga
    11761 tgataatcga cgacggcctc ttccccggcg ggatggccac ctccagcttc gacggcgagg
    11821 gggtcccctc ccagaggacg accctggtgg agggggggat cctccgggcc tacctctacg
    11881 acacctacac cgcggggaag gagccgacgg agactcttag cacggggaac gccgaccggc
    11941 cggggtacgc ggggatcccg cggatcggga cgacgaacgt catcgtctcc tccgccgacc
    12001 ccgaggatct cctggcggag acggagcggg gctacctggt caccggcgtc atcggggccc
    12061 acaccgcaaa ccccgtctcc ggcgacttct cggtggaggc gaggaacgtc ttcgtggtcg
    12121 aagacggcgc cgcccaaaaa ccgatccggt ccctgatgct ggcggggaac gtcttcgacc
    12181 tcctctcctc gatcagggtc gggaccgacc ctcggatggt ggggtcgttt gtggtcccca
    12241 ccgtcaaggc ggagatgaag gtcgtcggga gctgacggga tggatgggta gggcggaccg
    12301 ggtctcaagt tacatcgtgt aaaaaattag atattggagc gattagtgat gatcatcggt
    12361 ggaccatcct cccagcttct ggggggtagg atagcggcaa ttctcaaaga agatctggct
    12421 ttgtgcgagt acagcacttt tcccgacggc gaggcttata ctcaggtctg ctccgacctc
    12481 gagggcagga tcgtcgtcgt ccagagcacc ccctcggacc gggacatcat ccacctcctc
    12541 cagctcctgg acatctgccg ggggaggaag atcgacctgg tcatccccta cttcggctac
    12601 gcccgccagg acaagcagtt caagcccgga gagcccctct cggcgagggc gatggccgtc
    12661 gccatcgact ccttcctcgc ggagggctcc cggatcttca ccatcaacat ccacgaccgg
    12721 tcggtcctca agagcttccg acaccaggcc acggacctcg acgcaacgcc ccttcttgcc
    12781 gacgagatca aaaagatggg cctcgacgac ccggtgatca tatccccgga caagggggcg
    12841 gtccggatgg ccaaggccgc cgccgagagg cttggggtcc cctacgacta cctggagaag
    12901 accaggcaca gcgggaccga ggtctccatg gcccccaagg agatcgatgc ctcggggcga
    12961 gacgtggtca tcctcgacga catgatcgcc accggcggga cgatggccca ggcgatctcg
    13021 atccttagag cgagcggcgc gaagcgggtc tacctggggt cggtccatcc ggtcctgacg
    13081 gggggcgccg tcctgaaatt gtgccgggcg ggggtcgagt cgatcatcgc gaccgacacc
    13141 ctggagaagg cggtgagcac cgtctcggtc gcggggctcg tcgccgaagc tgtccggtcc
    13201 cggtgatggc tgccggatca gaggaccgct gggctctcca agacctggcg ggggcgttga
    13261 ggtggtgcag agggaggaac gctcggggca tccgctgcac cctggacgtt ctgggggaga
    13321 acgccaggaa tgaggaggcg gctgcagggt cggtggatgc ctaccgctcc tgcctgacag
    13381 ctcttgagga agaggagggg ctttccgcct ccatatcggt gaagctgacc gacctcgggg
    13441 cgctcttcga ccggaggctc tgcctggaga acctgcgaac cctctccaga gaggccgcac
    13501 aagccggggt cggctttgag atcgacatgg agggaaagcc cctggtggat atcaccctcg
    13561 aggcggccct ggaatgcgcg gccgaggggc ccctcaccct ggccgtcgcc gcgaacttcg
    13621 accgggcggc cgatgatctg aaggccttgc ttcgaagggg gatccggccc cggctggtga
    13681 agggggcgta ccccggcgac gtcgccgact tccgggaggt ccaggggcgg ttgaagggtc
    13741 tggtggaggc ggccctggct gccgagaggc ccttctctct tgcgacccac gatccggagg
    13801 tcatagagtg ggccctgggg cggctatcag aagagaagaa cctcgtggag ttcggctttt
    13861 taatggggct atccgatctg accaaggaga agcttgcggg ccaggggtgg gcggtctccg
    13921 agtacgtccc cttcggctcg cggcgacggg cgtaccaggc gaggaggctg aagtacctgg
    13981 gggagctcgc ccgcctgggg cgggagccag ccccctagat cagctcctgc cctgccggga
    14041 gggggctgtc gatggcgaaa cggagggtct acctcctccg ccgcagagca aggcagaagg
    14101 ctagccccac cgccagcagg gcgaaggccg cccccggcgc cggaatcctc cccctcggct
    14161 ccccaggggc ggtgacgttc acagtcccgt tgattaccct tctgacctct tccccctcca
    14221 tctccgcgat ccccacctcg ctctccccct ccgccatccc ggctcggaag gtgaggttga
    14281 aggcggagca gcctcctggg acctcgaggc cgatcgttcc ctcctccgat aagtccggag
    14341 agaggccgca cccctcgtcg gccccatcct cgatcccctc gaaggtgagg cggcgcgggt
    14401 cataggtgag ggccaccatc gtcccgttct ccgacggcgg gatctccagg gggagggcga
    14461 acctctcgcc ggcccggacc gtcgccgacc cgacggcggc ggaccggagg ttcgatcctg
    14521 agacctcgaa gtagagcccc gtcgcctgga gggcgttcat ggcgttctcc accctcagcc
    14581 tcacctcata ccggcccggc ggggctccga cccggactag gtggggctcg atcccctggg
    14641 gcgagaacca gagcttgtga ctcctattgc cgaggactgc ggtcccttcg acgacctgga
    14701 agaacctgat ctcgctcacc ccggcctcga tcttgacgta ccttttccgg agggcctcgt
    14761 cggaggccat ctccgcgtat gtggcgttta gccccagcct ctcgtactcg gcgggggata
    14821 tgtagtagat ctctcctgcc gtgtcccgga tcaggccgat cctcccggag gtgtcgaagt
    14881 tcaccgagac cgggaccggc tcggcggtat cgttcagctt caggacccct ccctgaaggt
    14941 ccgactcggg aaggagggtc ccgtcgtcgc tgagggcggg aacggtggcg ttccactgcc
    15001 aggagtagag ccactggtcc ttcgccccga cccccgaccg ggtgaggtct ccctggatca
    15061 cccgccgacc cccagcgtcg acggtctcgg cggtgatgct gacgatatcc ccagccctga
    15121 tgaacgccga gaagttggcc accccccccg aggggaccgg ctcttctgga agataaatct
    15181 ctacaacttc gggaggtggc agtacgtatt cattcttgta gatgtaatac cggagggtat
    15241 cgttgtcggc gacggcgatg ttcatccccg ggaggatgcc gacgacggaa tcgcgcttca
    15301 ggttgatgta gtccatgttg accatgtaaa tcccgagatc gggcccgatt aaaacgacgg
    15361 tcatttcgcc gagctcgtca ccggccttta tggggagaaa ttggctatcg gatacctgaa
    15421 ataggccgtc aattatggcg aactgatcct caccgtcgct gaagatgttc tccacgtgaa
    15481 gggcgattat gggcaggtct ttgacgttgc ccacatctct cttgtaaata tacgtggagt
    15541 tcggtctcat caccgcagtg tccaccagct ggttgttctt cctcagttcg atgaagatct
    15601 tgtcgtcttt gacctcatct ttggtgacat cgcggatgga gagggtatac ccctccccca
    15661 ggtaatagag atctcccgcc acggccgtat ctctctggtc ggtgtcgatg aggatgcggc
    15721 cgatctgctc ttgggccagc atgctgatcg gcttcacatc caggcgggcg gtggttccgt
    15781 aacccacaaa ccagacgtct cccagaaagc agaccacctg atagctgccc caaggatcga
    15841 actcgaactc cacctcatgg acgaagcata tatactcagc tccatccacc agcctaggcc
    15901 cctctctggc gggggttctt atgtatctcc catcggttct gtgaattatc aagctctccg
    15961 aacccatttt tttatcatca tcaaaataaa aaccagtgaa gttctcgtcg ttccatcggg
    16021 cctgggcata tccgacaaaa tctccgacgg ccgtgcttac cggaacctgg gagctctcgc
    16081 tataaaccgg ccccctgatc tcgcggaggc cgtactcggc tatcaccccc agggggtagt
    16141 agatcagctc aggggtgtcc ctcaccctca gcatcaggcc tccagcgagg gggatggtgc
    16201 tcccccggcg gaggattatg tcttgggcgt tctccagctc gatcctcccc tcggcgaggg
    16261 ccgtcacctc catcgcgccg atctggtctc cagcctctat cttgaaggtg gggacgtccc
    16321 gggcctggaa gaggccgtcg atgtcgacga tcgccatctc agtcccctgc atcgccgcgc
    16381 tgacggaggc gaggatgatg ggaagacggt ggtctcccac cttgtaggcg taggtatccc
    16441 ccaccctcac caccgcccga tcgacctcga cgccgttctt cttcaatatc agaaaggcca
    16501 tcttcccatc ggaggaggcc cccgccacct cgatccagta gccgccctcg aggaagaggg
    16561 gcgccttgga ggtggcggtc tggatcccct catagtcgag gaggacctcc cggagctccc
    16621 cctcctccag ggcggagacg gcgtcggtga agttgctctt caggtagccc gcgaggtacc
    16681 tcttccccag gaaggcgacg gcctggtagc ggccccatcc ggggtagtcg aactcctcgg
    16741 tccagaccct gctcgaataa tagaggtgac cctccgggat ctgtctctct tcagcctccg
    16801 cggcgagcct ctcgcctccg aacccctcgt ccaggtcgta gtagaaccat ccgaagctct
    16861 cggcgtccca gctgccgttc ccaccggaga aggtgccccg gacctcggcc atcggcacct
    16921 gccccacggc ccccatggtc atggtcaata gatagaagac cgccccgacg gataggaggg
    16981 ccctggagcc gagggttccc ctctctcttc taccggcgat agatgctaat ctcatacccc
    17041 cgaactagat ctgatctccc ctgcctgccg actcttctct tacgtcccct ctggataacg
    17101 tgtatatccg atggcgaagc tctgggcgtt ctggagatag aacatgtccc aggttaagca
    17161 tgttgcgata tacggcaagg ggggaatcgg aaagtcctgc accgcctcca acgtagcggc
    17221 cgcctgtgcc gagaaaggtc ttcgggtgct gatggtcggc tgcgacccca agagcgactc
    17281 gacgataacc ctggtcggaa ggaggatccc gacgatgatg gacctgatca gggccggggg
    17341 cgagatcgcc gaggaggacg tcgtcttcga ggggtacggc ggcgtcaggt gcatcgaggt
    17401 ggggggcccg gagcccggcg tcggctgcgc ggggcggggg atcatcgtcg ccatcgagtt
    17461 tctcaggaag gtctcgaagg tcctggagga gattgacctc accctttacg acgtgccggg
    17521 ggacatcgtc tgcggcgggt tctccgcccc catcaggaag gggctggtca ccgaggccta
    17581 catcataacc tcaggggagt acatgcccat ctacgccgcc aacaacatct gtcggggact
    17641 gaggaggctg aacacccccc tggccggagt gatatgcaac tcccgggagg cctcgggcga
    17701 gagggagatc gtggagaagt tcgcccggga gctcggaagc gagatggtcg cctacatacc
    17761 gaaagatccc atcgtccaga ggtgcgagag gcggggggtc tcggtgatcg agggggcacc
    17821 cgagtcggat attgcccaag tctaccgaaa gctggcggag cacgtcgctg gggactcaaa
    17881 gccgaggcat ccctcgccca tcgatgacga taagctccgg gagctgtccc tcatatgagc
    17941 tctgagatcc cgcgggtcct cctggcgggg gaccgaagct ccagcggcaa gacgacgatc
    18001 gtggcggggc tcctctcagc cctccggaat agggatctaa cggtccagtc ctacaaggtc
    18061 gccctcgact acatcgaccc gagctaccac accgggatca ccggccggtg gtgtcggaac
    18121 ctcgacggcc acctgatgcc ggaggagacg atccgggagg tctacgccca cgccgccgag
    18181 ggggcggagg tcgccgtcat cgagggggtc cgggggctct acgaggggta cgacggcgac
    18241 cgcggcagca ccgctcagat cgccaagatg ctgaaggctc cggtgatcct ggtggtggac
    18301 gcaaggtcga taacacgaag cgctgcggcc ctggtgaagg ggtacatcga cttcgacccc
    18361 gccgtcgata tcaggggcgt catcctcaac aaggtcgggg gcggtcgcca cgccgagaag
    18421 gcgatctccg cgatccggga tcacgccggg gtggaggtcg tcggcgtcat cccacgggac
    18481 gactcgatgc acctagccat gcgccacctg gggctcgtcc cgatgctgga gggggagaag
    18541 aggcacgcgg ggttcgccga gcggctggat aagattcgcc gagtggtgga ggagggtctc
    18601 gatatcgacc ggatcctgga gatcgccgcc gaggcggagc ccctgccgac gccggagctg
    18661 gacctttacc ggcccagccg ggagggcgac ggcgtcagga tcggcgtcgc cctggatgag
    18721 gccttcaact tctactaccg ggacaacctg gagctgatcg agctatcggg ggcggaggtc
    18781 gtcccggtga gcccggtcca cgaccagagc ctgccggagg tggatggcct ctacatcggc
    18841 ggggggtatc ccgagcttta cgcaaaggag ctatcggaga accggtcgat gatcggggcc
    18901 atcagggggg cccacgagga gggcgtcccc atcttcgccg agtgcggagg gctgatgtac
    18961 ctctgtgagg agatcgactg gggcggagag tcccgcccgg ccgtcggcct ggttccggca
    19021 aagaccagga agggggggca aaggatcgtc agctacaccg agggggagtt tatccgggac
    19081 tgctccatgg gcccccgggg cgcatccttc ctcggccacg agttccacca ctcggagctg
    19141 gtcctggacc cgggagcaga ggtggagtac gccatccgcc tccggcgggg gacggggatc
    19201 gatggggtga gagacgggat cgtcgatggg aacctcgtcg cctcctacaa ccacttccat
    19261 ggagcatcct atcggctctt cccgagccat ttcataaagt ccgcccggga ggcaagggat
    19321 aaataaactg ccctcttttt gggtttggtg atgataaatg gtgaagatgc cccccaaggt
    19381 gagagagacc ctggagaagc agtggccgat ccccttcgcc accgcctcct ctgaaggaaa
    19441 gccgaacgtc gtcttcatgg gaatcctgaa ggtaaaagac gacgagaccc tggtctttgc
    19501 cgacaacttc ttcaacaaga ccgcgaccaa cctggcggag aacccacagg cctcggtcgt
    19561 ctgctggacc aaggacccca ggaggtccta ccagatcaag gggagggtgg ccttcgcgga
    19621 ggaggggccg atcttcgagg agatggcgga gtcggtccgg gcccgtaagc ccgacctcca
    19681 gatgaggcgg gccgtgatct tcaaggtcga ggagatctac gaggctaact ccggccccac
    19741 cgctggggat cggatcgcct gatcccggca tccaggtgtc ggcaagggca gggccgtcgc
    19801 cccagtgatg cggggtgagc cttctattct ccttatttca tcaggaattt tcacggttgg
    19861 ctcttccgcc cttcaggccc tctcttcggc cccatccccc ccggcggaaa gagtaaatat
    19921 attgctattc tatttgaaca aaggtgatct aaatggtgaa gatgccacca gatgtcagag
    19981 caaccctgga gaagcagaag cccgcccccg tcgccaccgc cgacgcctcg ggaacgccga
    20041 acgtcgtcta cgttgggttc ttgaaggtcc tcgacgacga gacgatcatg atcggagaca
    20101 acttcttcta caagaccgcg gcaaacctcg ccgagaatcc caaggtatcg atcctctgct
    20161 acgacaccga gacgaagaag tccttccaga tcaaggggac tgcgacggtc cataaggagg
    20221 ggaagcactt cgacgagatg accaagtggg ttctcgccgt caacaacaag cttccggcga
    20281 aggcggccgt ggtggtgaag gtggaggagg tcttcaatgc catgtggggg ccgacggcgg
    20341 gaaagaaggt agtctgatgg ggcggcttcg ggatctgccc tagccatttt ttccttcggg
    20401 gcctccatta gaaggtggct tttttcttaa gaagaagcct cagagaccgt ttaaggtgat
    20461 gggaggagat tgaccttgaa agaggataaa gccatcagat ccagcgcagc gaagctcttt
    20521 cgcctctccg aagacgagtg cgacctggtg aaccggatcg tcctcgagga gatcttggcc
    20581 gagtcgaagg acacccacga gttcgtccag aggaccctcg accggttcgc cgggaccgag
    20641 ggggagagga gggccttcct cgcgggccac atcaccggct gggtcctaat gaaggcggat
    20701 atggacatgg atatcgactc ttacttcgcc gactcccggt cgaagggccc tgcggggcag
    20761 aacacgtaag cggcaagcag aaccagcgcc cgcaaaaaca gggcgatcga ggggaagggc
    20821 gcgaaaaaag ggaagacaaa ggggaagggt ttttcagcgg gacggatctt tgcataccct
    20881 actttgagga cgagactccg actccggacc gggatgaacg gccgggatct gtggcggctc
    20941 tggtgcgacc agttcgacga tgagctggag gctttggccg aggcgatctg ggccgcaaac
    21001 aagatggtga agacggaatc cccccaggct agggaccggt tctacgccat aaaggacgag
    21061 ttcatcctca ggtacgccac cagcggccgg aaagtcagag acgagccgcc cccaccctgg
    21121 taccgcgggg tcgacggctc gaccaggaca ctatactgct accgggtccc tgtgggcgag
    21181 agggtctatc ggctccacag ctacctccgc cccctaaagg tggaggaggg gctcgccgag
    21241 gagggcgagg aggggggcgg ctcctccgtc gacgagtggt cgtggcttcc gatctctttc
    21301 gacgagttat ataagatgct cgcgagctac gccggggatc ggtggggctt ctcggatcgt
    21361 agggtgggat gaatcgagaa gaggaagata gggaaggggc gtcagaagct atgaaaatga
    21421 agactggagg tgccggtttg gggcggccgc gcccgcagtg ggtcgccctt accccggcct
    21481 ctaaggtccg aggctcagga ccgcccacag caggttgaag tcattctgac gcgccctcac
    21541 catctcgacg aagctcggct cggcccccac ctcccccccg atggagaccc atcggtcgag
    21601 gccgaaggag taagcatgac ctccatgata accgtcgtac ctcaggaccg gccccttgac
    21661 gccttcgaac ctctctccgc cgacgacccc gccgtaatac agcccggggc cgtgaacccc
    21721 ctcgaacatc gccgatgcgg ccggagccag cagcgccagc accataatag ctattttgta
    21781 tcccagcatt cgcatcacct tcttatcgag catcaaccat cctcgatcta tgatccgcca
    21841 gagctgccag acccttccag taactgaagg tcctccatgg cctttttaat ccggcgggct
    21901 tcctgctata tcatgacggg ataggcgaaa aatgtttcga tgattgggca acccggccta
    21961 aggtttcgcc tggccggggg cggccgagcc ccatcaagtc tcaccaccgc tgggacccgg
    22021 gggcctctcg tcggagggtc ctttcgacgt cccggccatc tcctgggtgg gtccaggttt
    22081 ctcttttttc aaagatcttc ttttgcccat ccaccatttg acgattggaa ttatcaagac
    22141 ggtggccagc cactgccaat attttttaat aaaatttttg ccgtcgttga ggtagtcgga
    22201 ggcggtcatc tcttcgacgg tcaccgtgac cggccactcg tcgagcctct tcttaggcgc
    22261 ccacccagcg accccaatat tcgagatatc cttgtaatac accaccagct tcagggtctg
    22321 atcgccttct ttggtgggta ccacctccca gacccacatg ggcagttccc ctatatcggg
    22381 gcagtacatc tgttgagcct tcccttcctt gagggagatg acgaaattat ctcccgtcag
    22441 actgacctca tactcgcact tgggcgaaag gttgacctcc tcgtagatgg cgtattcttc
    22501 ctcgaggtac cccgctaggc gctcccgatc ttctcccaaa gttacattta ttttatactt
    22561 gtggcgtaca atcatctctc tgggcggatc ccagccgacc atcccgaggg ccttctcagc
    22621 catcttctga taatcttccg aggagggctg agcgggagcc gttgcttctg tcgtcctctc
    22681 cgagggcggc tgatacggta ccccctttcc gatcgttgtt ctgacgtcac cagggacacc
    22741 gcccgtatat accgttctgt cgaccggggt cgtgtccgtg gaccgtatct cgacggaagt
    22801 ctcacccttt ggataccccg attcttcccg gatgcagttg ccaaaatacg tatcgttatc
    22861 gatcagcacg tcgtacgtgt tatcccttgt aaaattatca tctactgtat tattctccga
    22921 ggaattgaac gaaacgccaa cttcattttt attaatgtaa ttatctttta actcgttgag
    22981 attcgactgg tagaggtaaa ctccgattcg gttatagctg gcggtgttgt ttttgatcag
    23041 gttcgatgac gagtcgcaga gggcgatccc atcagaatta ttggagactc cattatcggt
    23101 gaggttgttt ttcatcgagt ttatgagggt gatgccgcag gcggagctgt cgttggcgac
    23161 gtttcctgcc acggaactgc tgcgacaatt cataagtgcc aggccgttca cctcgttccc
    23221 gtggaggacg ttatcggcga tcccgttctc atgggaatcc tcaagcttga tgccggcgag
    23281 gtcgttgtag ctggcatcgt ttccggtgat gtggttgcga tcgcttgcag acagacggat
    23341 tccatactca acgttatggc tgccgatgtt cccggagatg gtatttccat cggatgactc
    23401 aaataaaatg ccgtttaaat tataactgcc gttattggcc tctatcctgt tatcctcgct
    23461 ctccctgatc tctattccgc tctgatcatt gtagctggca ttattatcct ttattatgtt
    23521 ctcgttcgat tttacgatga agattccaaa cacgttattg tagcttgcat tgttcttcga
    23581 gacgttgttt ccgaggctgc ttaataggta aaccccgata tcatcgaagc tcgcattgtt
    23641 ctcgttcacc gtgttgtaat gcgagccctt gaggtaaacg ccgcctttcc tgttatccgg
    23701 ggagacgacg tttctcttga tcgtattgtt gccagaatct tccaatttga tcccatagtc
    23761 gctcttttct atgacgttat cgatgatcgc atttcgatat gctccgataa gtccgattcc
    23821 atccctcaac ccgctcccac tattatctct gacctcgtta tccatcgatc ggtcgaggaa
    23881 gatgccagtg gcatcgctgc catcgttgct gaccccattt ccggcgatcg tgttgtagga
    23941 agaatcctgt aataagatgc ctactttctg gttatcgacg ataacgttgt cgtcgatggt
    24001 gttcccttcg gatcggacga ggctgatccc cccgcttcct tcgcttatgt tgttcccttc
    24061 gatcgaatta ttccgggatc tgttgaggtg gatgccgtcg gcgtcagaat ggccgatgat
    24121 gcggttggcc ctgacggtgt tcttctcgga ctcatgaagt tcaattgaat ttttattttt
    24181 taatacgtta tctgctatcg tggagttggc cgtattatat aaccgaattc cgtatccggt
    24241 ctcatcgaag acctgttcct ttattgtgat gttgctgcag ctgaggcagt agacgacggc
    24301 agcatcctcc gaggtcgaat cgatggtcat gttggccgct aaaaccagat aataaatggc
    24361 gcctccatca atccggttgc tggtatctat gtaatttagg ccggcggcct cgaagctccg
    24421 accgttctca tacataacgt tcttcctcag ggtgttgtat tgggacctct ccagggagat
    24481 gccgtaatcg gcgttgaagc tcacattgtt cccttcgatc gtgttattcc cggagtcctg
    24541 gagaataatt cccaggccgt tcttggtggc attgttcccc tggatgtagt ttccctggcc
    24601 cctcaagatc aagatgccaa atccgttatg gttgacattg ttcgcctcaa cggagttgtt
    24661 catcgagccg ttgaggacta tcccggtccc cgcaccatca ttatatatag taatatttat
    24721 tattttatta ttattagaat acactttcac tcctgccgcc tcttctggag atctagtgcg
    24781 gctgtagttc gtggcggcga atccctcaac cacgaccccg tcggcggaaa gggttatcga
    24841 gctcccgttt cctcccccgt ccaccactgg cagcccgacc cccctcaggg tgaggggctt
    24901 attgacctcc acgttttcgc ggaaaggatc tcttcccccc tccaccagga tcgtatcccc
    24961 ggggctcgcc agctctatcg tctcctggat cgattcgctc tgactcacaa cccaggtctc
    25021 tccttccgcc aacccaaaaa aaagaattga aatggccaac atcaagcttg cgaggtcgtt
    25081 caacggatat cagctcccca ttatattata atatattaaa tataatcttt tttgaggctc
    25141 ccataacaga cccctctcga tcgagccatc cctctgccat cgtacgactt atatgtcccc
    25201 gttatctggc ttccaatgac cgatagcgtc atctcgtagc ggaccatgtc gttcagagag
    25261 acgatgctca attgcaaatt cgtctcctca aacgatcccc tggcctccac cggttgctgg
    25321 gacggggttg ggagggcgtt gccgaggaaa gcttctgcca catctccaaa gatgtcgtct
    25381 ccagggcact tggacctcga agaggccgat ccgtcgatat cttcttctgg taaaaaatat
    25441 aaatttccat gaccggagat gacggtctca tgctggatca gcttcaactc tgcctttgct
    25501 tcgccggatc catctgacaa ggtcagatcc aaggaccagt ttattccttt gggtgtggta
    25561 tgtctggtca ccccgccgtc tatgcggagg atctctatat ggaggggcgc cctcgtctcg
    25621 tcttttaatg tatccggaag attattcaga acgttaacta tagatccgtc gatgttcagg
    25681 accctgccga ccagctcttc cggatcctct ggggtgctat ctcccgtata cctgaggatc
    25741 tccttcatat cctcggcttt gagactggga tatatcgacc tcatcatcgc cgcagtggcg
    25801 gtgacgtggg gcgccgccat gctggtgcca ccagattcat taattatttt ttcagtatca
    25861 tttcgtcccc agacggcctg agcgccgggt gcggcgatgg tcacttcaaa atagtaatct
    25921 atattctggt tgctgtccgg cgctggtcta cgatcgttca tgacgttgcc gacggtgatc
    25981 atattggaga tatccatgcc gcctggagca tgtttgtaat cactgcttgc cctcttcacc
    26041 ccgccgtttc cagcgctgca tacgaacaat ctccccgggt ccatcttaag aatatcttta
    26101 aagaacttat tataggccga gttcaatgag ttgttcgcct tatcgtttcc ccaggagcag
    26161 ctaatgatgg tgcttccgtt ctccaatgcg tacttgagag atagaaatgc gtcggtagaa
    26221 aaacttttct gattagatga aaatatgtta tataaattaa tctccaattt atcccccagg
    26281 ggttgagacg ctatgcccac aagccctcca tcattgggat ctgcggccag aatgttcatg
    26341 acgccagtgc cgtggctccc ggcgatctca aaacccggca ggggttcctc cagctcgggg
    26401 ctatcgaatg cgcaccctcc aacagaagtg ttgatggcgg catcatcaaa ctcacagcaa
    26461 tttttccatc tgtatattcc gtcatcgatg atcgcaacct ttacgggaaa taacctgaca
    26521 ccggattcgt tgatgctgtc ccaggcttcc tggacgcgga ccatctcgta gcccctgcag
    26581 ttctcccccg cataaaccga gtcgcttagc ggagattctc gatatatctg ttggtttgga
    26641 aaggccagct cgacggaggg atcctctctt gcagtggcga tgtctctaat tagtccttca
    26701 atgctgtccc ccctggcctt tgtccggttg gttttgatct ggaacattag tatggtttcg
    26761 aactggccta ccaccgatcc atttagacct ttagccagat cttcagctat gctggtcgcc
    26821 tcttcgaacg agaaccgttc gtccactacg accaatacat gatttatcgg gacctgccca
    26881 ccccaatcaa tcccctcagc ggttccatcc accagctctt cgggatccgg tcgctgaaag
    26941 ccgtcctctt cgcctagaat aaggggtctc tcttccacgt agaccaggtc gtccttccag
    27001 ctccctttgc tataaaaaat ctcatcggca gagtgtctgc cgtcggtggc gtatgcaccg
    27061 atccggtaga ctcctcgttg ggatggacgc cactcccatt ttgcctcagc ggaccagttc
    27121 tgctggagct cccagtcgtc cccggtcgat gggccccgga gccagaagca gtagaagacg
    27181 ggatcgcctt cgggatcgga agcagaagcc gtccagatcg caccccgatc caccctcagg
    27241 gcagtggggt ctggtcccag agaggtcacc gtcggaggct ggttcggccg gcaagttaat
    27301 acgaggtcgt aaagctcgac gtacgccccc tccagagtct cgaagtcgat gatccccggg
    27361 ggcagtggat ccggatctgc ataatcgatc acctgcggaa gggcggagat tttccccgcc
    27421 atcatctccc catcgatctc tctgatcacc gtcttatccg cagcttctat cctctctata
    27481 tagacctgta tatgtccttc cctggagatg atggatacct gatgctcttt ggtcgggtcg
    27541 tatggtattg aattgccagc aaatttttct gggggttctg tctctccaac gtatttgaag
    27601 ataaatgtag aaagggtata atcgtccgag ggtattaagg caacgaaggc agcgggagca
    27661 tctttcgcca gagatggggt attggtaaac cctatcgagt agcgatttga gccgtcgatg
    27721 ttgatgttga cgcgcatctc gccagtgaga tttttgagct tgaagctgag gttgaggtcc
    27781 ccctcttcgc aggactcgtt atatcccgcc cagctcgcgc cgtagccggt gaggttaccg
    27841 tcgtcgatgg tccagccagc catcctcaca gcggtgagag ggccgccgaa ctcccgagca
    27901 aggccgacct ccagactggc ccccagggat aagcccaaaa ttattatcag gatagacctg
    27961 atgagcaacc cctccgtctt catagcagtc tcctcgcaac gtcgtctcct gaaattttat
    28021 gtggcccctc cggaagatgc catcgtcggt gggctcaggt cgtaaccgcg gcgtcaacgt
    28081 cgcatcgccg gtcaggttcc atccttggcc atcgctcttt cggtatcttc tatcctcagc
    28141 gggattatac cagagaatct tgctccggcc tctcgaagta gccgaccccc aggtccagat
    28201 tcccctccgg cttctccgag gccgtatcct cttccgttat tggggatgga catacgacgc
    28261 tgacgttgga aaaccggacg aaggtcccgt tgagggcttc aaagtcgatc ctgccggggg
    28321 ggagggggtc gggatcgagg taatcgatcg ctggaaaagg ctccccagtt tcccggtccg
    28381 gctcattgaa catgacccgg atctctccat tatccgatag gatctccacc tcatactcct
    28441 gggaatggtt gtaggctacg ggctctccgg ccacccattc ttcgggggag ggaaccgtcc
    28501 ctgcctccct gaagatgtaa gttgatagta gcccgtcatc gtcgctcttg agcccgattg
    28561 cgtaccgatt ttggccgctg acgtttacgt tgacgtgcat cccgccagag aggtctttga
    28621 gcctgaattt gaggattagc gggccctcgc aggattcatt gtatgccgta aagctcccct
    28681 cgccggcgcc gatgatctct ccatcgcagc tcatccatcc ctcctcgggg gaagaagtac
    28741 cctcatcgca gtcctcaggc ggcgcagcca gcgccgatgc aagaaccgcc gacgaggctg
    28801 cgctcagaat aacgagcaag gcggtccatg caaattttct gtcgacaatc atgatccctc
    28861 tcctgcatca gatgcgtatt attatgaatt acaacggtaa ataactttcg atttaaaggg
    28921 aaatcaaaaa taatatacaa ataattatat ctaataattc gcggttatta atccgggaat
    28981 tatcgatcca tatgggaaaa aaaaatccga tattataccc ttggcctcag gtccggaggt
    29041 gaaatgaacg cttctaaaac cttttctccg tcaagccaca ggatcttcgg gccgatgccc
    29101 taaatttatc gaataattcg ggttggaatc aattaataaa tatcggcata ggcagatcgt
    29161 aaaattgatc tccgctccga gaagctaata ctcttccccc aacgcttata agttctcgga
    29221 gagagagtcc caaactgtat gccgttctgc agcgtagaag aggcgatcga ggaggtgagg
    29281 g
//
LOCUS       DASM01000133            7872 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_5196, whole genome shotgun sequence.
ACCESSION   DASM01000133 DASM01000000
VERSION     DASM01000133.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_5196"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    2123..2212
                     /estimated_length=90
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1436 a         2488 c         2461 g         1395 t
ORIGIN      
        1 aggccgtctc cgaggggatc gtccggaagt cgacgaacct catctgcacc atctccgacg
       61 accggggaga ggaggtcctc tacgccggaa agcccctcag ccgggtcctg ggagacggga
      121 tggggatcgg cggggtgata ggcctccttt ggttcaagaa ggagatcccc ccctgggcgg
      181 cagagttcat cgagctcgtc ctccagatcg tcgccgacca cggccccgcc gtctccgcgg
      241 cccacaacgc catcgtcgcc tcctgcgccg gaaaggacct gatcacctcc ctcgcgagcg
      301 gcctcctcac catcggcccc cggttcggcg gggcgatcga cgacgccgcc cgggagttca
      361 agagggcccg ggactcgggc ctctcccccg aggagttcgt ccgggggatg aaggcgaaag
      421 gggtgaacat tccggggatc ggccaccgga tcaagagcgt caagaacccc gacaagaggg
      481 tcgagctcct catcggctac gcgagggaga acttcgccag gaccgacctc ctcgaatatg
      541 ccctctccgt ccaggagatc accaccgcca agaaggggaa cctgatcctg aacgtcgacg
      601 gctgcatagg gatcctcttc atcgacctga tgagctcctg cgaggtcttc gacgagaggg
      661 agatcgacga agtcatcagg tacggctacc tcaacggcct cttcgccctc gggaggtcca
      721 tcggtatcat cggccacatc ctggaccaga agaggctcga ctcgaggctc taccgccacc
      781 cccaggacga catcgcctac atgcttcccg agctggggtg agccgctctc agggttgaga
      841 atcgggccga cccctcccgc cggccccccc ttttacggag gttattactt tggctaccat
      901 cagcgagaag atattcagtc gggcctcggg ccaccgggcc gaggcggggg agatcgtcga
      961 ggcggaggtg gattacgcca tgtcccacga cggtacgagc gtcctcgcca taaaagcctt
     1021 caccgagatg ggatcggagc gggtctggga ccccgagagg atagtcgtcc ccttcgacca
     1081 catcgtcccc gccaacaaca gcaccgccgc cgacctccag aggatggtcc gttcctgggc
     1141 aagagatcag gggatcgtcc atttcttcga gggggggcgg gggatctgcc accaggtctt
     1201 ccccgaggag gggttcgccc tccccgggac cctcctggtg ggggccgact cccactcctg
     1261 cacctacgga gccttcggcg ccttcgggac cggggtcggg gcgacggata tggcggagat
     1321 ctactcccgg gggaggctct ggttcaaggt ccccgagacg atcgccgtcc ggatatccgg
     1381 ccgcctcggc gagatggtcc tcgccaagga cctgaccctg atgctcgtcg gggcgatagg
     1441 agccgacggc gccacctacc gggccctgga gtacctgggg gagacggtgg agagcctctc
     1501 catctcgggg aggatgaccc tctgcaacat gggagtcgag atgggggcga aggccgccat
     1561 cgtcccggca gacgggacga ccgaggagta cctgaggggc agggctaggg ggccctacca
     1621 gccggtccga tccgaccccg ggtcttacga cgcggagatg gagttcgacg tctcggatct
     1681 ctccccccag gtcgccgtcc ccttccgggt agaccaggtc cgccccgtcg gcgacctggc
     1741 ggggacggag gtcgaccagg tcttcatcgg gacctgcacc aacggccgcc tcgaggacct
     1801 ggaggtcgcc gcccggatcc tgaaggggag agaggtgaag gcccggaccc tggtcatccc
     1861 cgcctcgagg gaggtcttcc tccaggccct ctcctcgggg gtcctggagg ccctcgtccg
     1921 ggcgggggcg atgatcggga cccccggctg cggcccttgc ctcggggccc acatgggggt
     1981 catctccgag ggggaggtat gcctctccac cgccaacagg aacttccccg gaaggatggg
     2041 gaaggggggg ctcgtctacc tcgcctcccc ggcgacggcg gcggcttccg ccatctccgg
     2101 cgagatcacc gacccgaggg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcaccgac
     2221 cacgacaccc tggagggctc cttcgaggcc gagaggtccg tccgcaaacg aggcctccac
     2281 ctcctggtgg tgccaggggt cgaggtctcc acctccgacg gccacctcct cgccctgggg
     2341 gtccgggagc taccgccatc tgggcggagc cccgaggaga cgatagagtt cgtccgctcc
     2401 cggggcggga tctccatcgt ctcccacccc taccacccct tccgccactc catgtacagg
     2461 ataccgggct gtgacgccgt cgaggtcttc aactccaagt tcatcttcgg ccttgcgaac
     2521 ttctgggcga ggaggggggc gacgaggctc ggccttccga tggtggcggg gagcgacgcc
     2581 cactcggcga ggtccgtggg gctgggggtc accatcgtca ccgtcggcga ggacggagac
     2641 gtcttggaag cgatccggga agggcgcgtc gagatcgacg gcaagaggac ccctcctgtc
     2701 gccttcctcg cccagatggg ccgctgggtg aagaagcggc tccgagggag gggaagaagg
     2761 tagaagagga ggtaagggcg ggacggctga ggctcggata aaaagcgatc gaaaggtaaa
     2821 accgaccgga tgggatccgg tcgcttctga gtccgaaggt ttgagattcg agaaatgcga
     2881 gctacttagt cttctccacc ttttctgctt tggggcccct gtctccggcc accacttcaa
     2941 agctgacctt atctccctcg tctatggaga cgcccggctt gagaccgctc gagtgtacaa
     3001 aatagtcggt accatcgtct ccagctataa agccgaaatg cttcgttctg ttgaagaact
     3061 tcactgtgcc agtaaccatt tcaattcctc gttatcctga ctagaagcct acgactattt
     3121 aaaggtgctc cctccaggcc gcatcggcgg gcccggcgtc tccacatctc ccatcaccgc
     3181 cgaccggagc cctcgacggc gaagccaaaa aatatggagg caggatcccg ttcagtcgac
     3241 cagggggagg tcgtcgacgc tcccgccacc ggggatggcg aagacggcgt tgacgatgac
     3301 gtcgcagatc gtatcgttgt tcaggtcggt gcagttgaag gaggcgtaat ggttcccgat
     3361 ggtcccgtcg ttccacaggt ttacgccgtc gtcgaaggcg tccatatcgg tgttgttgta
     3421 gagcctgtta ttgaagacca ggttgtcggc ggagttagta aggaggatcc cgacggtgtt
     3481 gttcgagacg gtgttccctc gaagggtgca gtttctcgcc cccgccagga tgatcccgga
     3541 gcctccgttt ccgctggcta tgttgttcgt gatggtgcag ttacttgatt ggacccggat
     3601 cccggcgtcc cctgagaggt ttccagagtt cgtcaccctc atcttatcca ccaccgcccc
     3661 gtctgcattg acggtgatgc agcttccgtt gccctgggcg ttaatcaggg gcaggtcgcc
     3721 gctgtgccac atctctcccc tgaggacgat cggctttgtc acgttgacgt tcccagagaa
     3781 tgtcccgctc ctcaccagga cgacgtctcc tgggctggcg atgtcgatcg cctcctggat
     3841 gctgaagtac tggcagccac aggctttgca ttcgcagacc ttcagggtag ccgcccccga
     3901 ggccatccca ccagcgatga ggatggcgat aagggctaca gcagatggga tcgaaaagtc
     3961 tctttggatc atgaatctcc ctctggattg ttacgaccgt ctcagggaat ataaaaagct
     4021 ttttcgaggt ggctccccgc ctcgaataac tatataaccg ctcgaacccc tccagatccc
     4081 cgttatgacg gagcgcttcc ccccggcgat gagcgaccag ctcaaaaggc agggatatca
     4141 cctggtggcc cacggagccg tcaagccatg cctctggctc cggcggtcga tccgcggagg
     4201 agaccagtgc tacaaacacc acttctacgg gatctccagc caccggtgcg tccagatgac
     4261 ccccaccctc ctctgtaacc accgctgcct ccactgctgg aggcccatcg acgagatccc
     4321 caccacctcc ggggagtgga tcgagcccgg ggagctgctg gacgggatct tatccgagca
     4381 gcggaggctg atctccggct acggcggcgc ccccgagacg accgacctcg cgaggctcga
     4441 ggaggcgaag agccccgccc acgtcgccat ctccctgatg ggggagccga ccctctaccc
     4501 catgatcggt gagctggtgg aggaggttaa aaggcggggt atgaccgcct tcctggtgac
     4561 gaacgggacg tccccccagg ccgtcggcga gttccgaccg acccagctct acatcagcct
     4621 caacgccccc gacgaggaga cctaccggcg ggtctcaaac ccccgggacg atacctgggg
     4681 ccggtttctg gagagcctgg atatgatgag agattcacca tcccggaggg tggtgaggct
     4741 gaccctcgcc cggaacctga acctcaaaga cccccggggg tacgcgaagc tgatcgaagt
     4801 cgccgagccg gacttcgtcg aggtgaaggc ctacatgcac ctgggcctct ccagaaagag
     4861 gctcgggcgg gatgcgatgc cgactcacga tgaggtgatg ggcttcgcga gggagctctc
     4921 ggatatcctc ggctaccccc tcaaagacga cgtccccctc agcagggtgg ccctcctctc
     4981 ccgggggagc gccggggaga agatagagct ggaagccgga agatgaccga attgaagatt
     5041 ttaatttatt ttctataatt aatttagttt ctatcattgg catatcattg ttttctggtg
     5101 agtcctccaa cgggaaaagt atttttaggg gcaacatctc ggggttgtta ggagaacggg
     5161 atagatgtct gcggcgattc aggatcctaa aagctcggag gtgagggggg aggggccgta
     5221 caacaagtta aaaattttgc tcgttgagga caaccccgac gacgtcgagc tgacctgcag
     5281 gctcctccgg aggatctgcc tcgaaaggga catcttcctg gcgacggaag ccgaggaggc
     5341 tctcaagatc ctggagtcga tcctcgagga gggcggagcc ctccccgacc tgatcctcct
     5401 ggacatcaag ctcccccgcc tcagcgggat ggagcttctg gagcggctga aggccagccc
     5461 cgagcttcga ggtatacccg tggtgatgct gaccggctcc atcgtatccg agcacatcca
     5521 gaagagctac gacctcggcg ccgtcagcta tcttctgaag ccgatatccg aggacgagct
     5581 ccttctgacc ctcagctacg tctcctgatc cggatggatc gttcctttca agccccctcc
     5641 gaccgggagc cccaccgccc cggaggcgtg gccgaggagg gcgccgaaga tggcgtaggc
     5701 gaggatcatg taggccggca tcccgaagag gtcggcgggg gccgagtagg accaggcccc
     5761 ggcggagatg gggagggact ccaccagggg gcccagaagg gcgccggccc ccatcatcgc
     5821 cagagtcgac ctctcccgga aggcgaggaa gaccgccgcc gccaggaccg acccccccgc
     5881 cagggcggcg aggggtttca tcccgaggta gacgaagatc gcgatctccg cccccacccc
     5941 cgccagggcg agggctagag ctctggaaga agagccaccc atccaccccg gccccaggac
     6001 cgcccccccg atgagccata tggcaggagg aaaggaggcc caggcgggga agttccatgg
     6061 cgggacgtaa gggaggcccc cggtatcggc gtaggtccag agacccccgg cgacgcaggc
     6121 ggcctccgtc aggggcccga ggatggcacc ggtgagggcg agagccgccg ccctccgggg
     6181 gtggccgacc ctcatcccga ggatgaaggg cggcgggagg aggaggatcg ccgcgaggta
     6241 cggccggtcc cagagggcgg tgaggaggag ggtgcagtag agggcgagga tcgcgacgac
     6301 ggcgacgtcc cctctggggg agcggcccat ctaatccccc cccgcaagct tcctcaccag
     6361 ggcgatgttc ccctggtcgt ccctcctctc gtcgacgggg gtctccagga tcagggggag
     6421 ggcccttatg gcgggatggc ggaggatccg ccggaacccc tcctcgccga tctccccgag
     6481 gccaatgtgc tcgtggcggt cgagcttcga tgcgagggga cccttggagt cgttgaggtg
     6541 gacgaccttc agcctcgaga ggcccaccgc ctcattgaaa ccctccatcg tctcagccac
     6601 cccttccgcc gtcctcagct cgtagccggc gacgaaggcg tggcaggtgt cgaagcannc
     6661 tcatgatctc cgccaggtcc tcgaaggacc ctccgacgct gttcttcgag tcggaggtcg
     6721 tctccagaag gaggatgacc tcgttctcga cggaggagag ggccctggag atcgcctccg
     6781 ccgtcctctc aaaacccccc tccttgccgg agtcgagatg gcttccgagg tgggtcacca
     6841 ggtatgggat ccccaggatg gcgcacctct ccagctcctc ggcgagggcc tccacggatc
     6901 tggcgtaaac atcctccttc ggcgtcgcga ggttggggag gtagggcatg tggtctacta
     6961 ccggggagat tccggtgagc gctagcttcc tcctgaactc cctcgccacc tcctcgggga
     7021 tctccttgaa cctccacccc cgggggtttc gggagaatat ctggaacgta tcgcaaccgg
     7081 ccttttctgc cctgtccacc gccaaatcta tcgagccggc gatggagacg tgaaccccta
     7141 ccttcaccat ttcatcaccc tcctggggga tccggcgccc ggtgatccgg gcccttaaag
     7201 atcaattgga ttttgagggt catcccctcg acgatgaaag acgccccatc atcccaacgg
     7261 cctcgggaaa tgatcggccg aggagagggc gccggatccc agggaggggt catttgagaa
     7321 ctgttttata ctttcccgat gagccgggcc aggagaggtg gtgcggccct ccggccgccc
     7381 ctcccggcgc cgatgaagag aagagttcgc tgatccgatg atgagcccta tgagtcctga
     7441 ggcgtcaggg cctctccgaa ccagggagcc ccgttcaccg cccaccggcg ccctccagct
     7501 tggatctagc ccaccgatcg acgttgtcga tgaggttgaa ctcgaggatc ttcttggtgt
     7561 cgttgagcat ctccgccacc tcggccatct tgaacttcgc agctttgccg gaccggttta
     7621 ccacctcgat ctcctctttg aggtccgccg ccttcccggt ggcgaggatc tcggagaggt
     7681 cccggcaggc cttcttcagg tactcgatcg agtagtcggt catcgattcg cggtcctgga
     7741 ggtttctgtt gacctccttg atggaccagc agatggtaat caccttatcg tacatcttta
     7801 cccaccgccc gtctatcggc catatcgata tttaagagct ctcttcatca aaaatagtgg
     7861 ggctggtgcg at
//
LOCUS       DASM01000134            8846 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_58103, whole genome shotgun sequence.
ACCESSION   DASM01000134 DASM01000000
VERSION     DASM01000134.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8846)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8846)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8846
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_58103"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1559 a         2819 c         2839 g         1627 t
ORIGIN      
        1 tgagcaagtc catctccggt tccaaggaac tgatggccca ggtcgccgtg gactccgtca
       61 gcgccgtcgc cgagaaggtc aacgacaagt ggcaggtgga catggacaac atccaggtca
      121 ccaagaagca cggcggttcc atggacgaca cccagctgat cccgggcatc atcgtggaca
      181 aggagccggt gcaccccggc atgcccaaga ggatcgagaa ggccaagatc gccctgctgg
      241 acgtcgcctt ggagatcaag aagaccgaga tcgatgccaa gatcgagatc accgacccca
      301 ctcagatgca cgccttcctc gacgaggagg agcgcatgct gaagcagatg gtcgagaaga
      361 tcaagatggt aggggccaac gtcgtcttct gccagaaggg gatcgacgac ctcgcccagc
      421 acttcctggc caaggnntgg agaagctcgc ccgggccacc ggcggcagga tcgtcaccag
      481 catccacgac ctcgcggaga gcgagctcgg ccgcgctggc ctcgtcgagg agaagaagat
      541 cgccggcgac gacatgacct tcgtcgtgga gtgcgagaac cccaagtcgg tatcgatcat
      601 cctccggggc gggaccgagc acgtcgtcga cgagctggac cgggccatgg aggacgccct
      661 cagagtcgta ggggtggccc tggaagacgc cctgctgatg cccggcggtg gcgcccccga
      721 gatcgagctg gccctcaggc tgagagagta tgcggccacc gtaggcggcc gggagcagct
      781 cgccatcgag gccttcgccg aggccctgga gatcataccc aagaccctcg ccgagaacgc
      841 cggcttcgac cagatcgaca ccctcgtcgc cctccggagc agccacgaga agggggtcaa
      901 gaccgcgggc ctcgacatgg agaccggcaa gccctccgac atgcaggaga agggcgtagt
      961 tgagccgatg agggtcaaga cccaggcgat caactccgcc gccgaatccg ccgtcatgat
     1021 cctcaggatc gacgacgtca tcgcctccaa gtccggcggc cccggcggcg aaggcgggat
     1081 gcccggcggg atgggcggca tgggcgacat gggagaggac ttctgatccc ctccctaaat
     1141 caccattttt tataagccgt ccgttttaaa aaatgagcct cagagccccc tgcaggctct
     1201 ggtctcataa gcctcctcga tggcaggcct gtaaaacttc tcggtgtact ccttcaccat
     1261 cctccgtgag gtgaactggg gtccggtgac ctttatcgcc tccttcatca ccccgaccca
     1321 tccccggggg acgcccttgt cgtccacccg gtagtagagg gggatcacct cctcttcgag
     1381 gatctggtag atcgtctctg catcccgatc atccctgaga tcctcgctcg gaccctcacc
     1441 cccctcgaag ccccacccgt tcttgccgtt gtagccctcg acccaccagc cgtcgaagac
     1501 gctgagctgg ggtacgccgt tgagggcggc cttcatgccg cttgtccccg aggcctccat
     1561 ggggggccgg gggttgttaa cccagacgtc gacgccgtgg acgagatact gggcgaggta
     1621 ctggtcgtag tcctcgacga aggcgatcct cccctcgaag cccttgtcgg cggcggcgtt
     1681 gaagacctgc tggaggagga gcttcgactg ctggtcgtcg gggtgggcct tccccgcgaa
     1741 gatgatctgg actggcctct gggggtctgt gaggatcctc cggagccgtt cggggtttct
     1801 gaggaggagc gtcgccctct tgtaggaggc gaacctccgg gcgaagccca gggtcagggt
     1861 cgtggggtcg aggagggttc caaaggccat gatagtcctg gactcggact cgggatccct
     1921 ccacttctcc ctcgcccggt cctttatctt gtttatcagg gatcgtttca tcctgaggtg
     1981 gtccgcccag aggacgtcgt cggggatctc gtcgagaagc tcccatatga gggggttgtc
     2041 gtgcttttcg agccagtcgc ggccgaggta ccggttgaag gtgatgttgc cgagccgccg
     2101 gtcgatccag ctggggacgt ggatgccgtt ggtgacgtga tctatcggta cgtcctctgt
     2161 ggagtggtcg ggccagaggt ggtgccacat atccttcgtc acctccaggt gacgtctgct
     2221 gacgcagttt cggtggtggc acatcctcag ggcgaaggcg gtcatgttga agccgctggc
     2281 ggggtctccg ggatcgaagc ccagccggaa gaagtcgtcc cggctcatcc ccagccgacc
     2341 gaggtacgag ccgaagtact tttccatgag agagaggggg aacttatcgt ggcctgcggg
     2401 gacgggggtg tgggtggtga agaccgtcga cctcctaacc acctcggccg cctcgtcgag
     2461 gctcatcccc ctctcgaccc cctcccggat ccgctccagg atggcgaagg ccggatggcc
     2521 ctcgttcagg tggaggacgg cggggtctat ccccagcgcc ctcaggaccc tcacaccgcc
     2581 gatccccagg acgatctcct gcctcagcct ctgctcggag tcgccggtgt agagccggtc
     2641 cgaaacggtc cggttccagg ggtcgttggc gtcgacggcg gtgtccatca gatagaggtc
     2701 ggtccttccc acctggacct tccagacctc gacgaagatc tcgccgtcca ggaggggtat
     2761 ctccaccagg aggggctcgc cgtcgtcgtc ccggagcctc cggatcgggg cgttctctcg
     2821 gtcgagcttc tcgcaggccc cagcctgcca tccgtcgggg gtgatcctct ggaggaggta
     2881 cccctccggg tacatgaacc cgaccccgac ggcgggtacc cccaggtcgc tgccctcctt
     2941 caggtagtcg ccggcgagaa acccgaggcc tccggcgtag acgggaaggg cgtggtggag
     3001 gccgtactcg gcggagaagt aggctatcgt cttatctttg ggatctccta tgttgttgca
     3061 gaaccagccg ctggtggggt ccatgtcccc ttcaaacctc gccatgacgg cgtcgtagta
     3121 cctcagaaaa cgggggtcga gggaggcgag tctcagaacc ccgtcgtcca gctcatgcat
     3181 catcttgacg gggttgtggc cgtttcggct ccaggcggcc ctgccgccca gcatcatgaa
     3241 cagctccctc ccctcagggt gccaggtcca ccagaggtta taagcgaggt cgccgagctt
     3301 ggcgatcctc gccggcaagt tcggaaattt gtctcttggt ctatagtgca tgatctctcc
     3361 ttcccatcac cggtctctct taccatgggc ctaggatgat tttacctttt ccaccaccat
     3421 ggagagagca aggagaaggg ctcccgaccg ctttgggatc tcggatcagg cctgaaagtt
     3481 cactcatgac aaaaaaaatc ccggggaaga agatgagccg cgaaataggt atgggcgggc
     3541 gatggttcac ccgggcccaa gcaccgtctt tcttcgtcaa ccgaagctga gaaactgaga
     3601 tctacatccc ttcagtgggg acgagctccc ttccagcgtc gacccatgcc ggaattccgc
     3661 ctaccactac ccacgcgtca cggtcgccga atatcctcat agccgctacc gcaaaggcag
     3721 agttggtgtc aaatgtacat acaacagcga tggtcttgtc ttccgggacc atatccatat
     3781 ttggaatcaa ctccgggaag ggtacgttga tcgaggctgg tatgtgacca gagtcatagc
     3841 ctgcggcggg cctcacatcc acgatcacga tgctctcgtc accggcgtca acggcgttaa
     3901 taaggtcttg catctccatc aggaggaggt tgttctcagg gaggctcata aagaaggcgt
     3961 cggcctccgc catgaagtcg acttctgccg tctcgtcggt ttcagccatt tcttcgactt
     4021 cggccatctc tccatcctcg tctccagcct gaactgagaa tgccgccaca aatagtccca
     4081 ggaggactaa aagaaacaga gttttgttct tcatgcgata ccacctgagt atatatttta
     4141 ttataatggt atatatcatt tgcggacata ttgaattaaa tgattcttct aaaattaaaa
     4201 tttaatctta tgcgattgat tcgcccaacc ctgatattct ggagatgcaa aatagattta
     4261 ctttcaatgg gatcggtcac cggtccggga tcggatatgc tgggtaagtc tccatcctcg
     4321 ataaaggcac ctctcttcca gcggcgaagg agtagagggg cgcaagctcc gtccccgaga
     4381 ggcccagggc ggaggagaga gggtcgtcga agtagcatcc tatcccgcac cctccgagcc
     4441 ccgcggcctc agcctccagg tagagggtct ggccgacgat tccgcattcc cagaggaggc
     4501 ggcagtagaa ccaggggccc cgctcctcaa ggcaaggccg gaggctggcg atcatggcga
     4561 gggagaagca gccgtcagag gcgatcttct ggccgcaaga gagggcccgg gacaggtcgc
     4621 ggaagtcgcc cccttccagc atgtagaatc ccggaggcga tccttttggc ctctcccagt
     4681 cgaagctctc cctggcggct gactggagga gatcttcgtg gtcggggttt cggacgagaa
     4741 agtacagccc ccgatcgagc cccaggaccc ggtggacgaa gatagcgagg tgtacctggg
     4801 gcctccaggg gaggaggttg gtggggaacc ttcttcgtcc tggcagagtc cgggccaggg
     4861 ctctgaatag gtcatccctg gtcatggcgg aggcggggtc catgacgatg acgctcctcc
     4921 gctctcggat gagcttgcgc aaggaagatg acgaataagc gagcgcgggc ggcgtctcat
     4981 tctccatcgg ctctttccgg tcgagctccc cccgaggttt tgccgtagac ctagccattt
     5041 cggcgaggag gggccagcgg acgtggcccc tgctgagccg gtccggcctg cccaaccact
     5101 ccacttcccc gaactcggcc aggaccctct ccgggagggc gaagtctgac aaatccgggc
     5161 cggagccgcc gacctgagga tggatcgcga cgaggcagtc ggggatctcg gcctcggtct
     5221 cgccctctcc atccccctca tcccccgggg accgatccct cccctccgcg ccgctggagc
     5281 ctgccactcc cagcaggagg gccaggtcgt cggtgccgac gccgtcgagg agggtcgctc
     5341 tccaccccag ggccgccgag gcgatgctca aggcggccag ggcgtggccg aggttgaggt
     5401 ggaggtagcg gaaggccctt tcgccgtact tccaggcctc ccgccagggt atcgaggtta
     5461 ggccgacgag gaccgtcccc tccggcaacc tcatctcgcg ccaggcctcc tccgagaacc
     5521 gggacctcac ctccaggccg tgctcccggg gggcgtagtg ggcgacggtg ggcctgtcga
     5581 agagccccgg caccggcccc gagatgaggt acccctccgt cgggtggagg ttcccgctgg
     5641 agggattgac ccgcagagcc caggccgcgc cgccagcccc ggcccgcctc caggccgaaa
     5701 tggcgaggga gtcgaagagg agctgggaga cggtctcaaa gttcaaaagc cggggcggga
     5761 cgttcccctc ggtgagggcg aggtcgtacg ccggcccctc gccgggcgag ggctcggccc
     5821 gggccagctc cactagcggg gcgccccgat agcgccgaaa gggggcgggc tcgttcgccc
     5881 agtccatcgt catcggcccc ggggccattt ttgtgaagct gtgctttgtc gcctggtggt
     5941 aggcgaggac tatgtcgagc tcataggaca attcgagatc ctcctgggca ggtcggacgg
     6001 ccagttcagg ttatgggtca gcttcctcca tcagactttc catcccctcc gttcatccat
     6061 tcgatatatc cagatccccg tcgccagggg tgggctcctc tcacccgatc tcggccggcc
     6121 acccttcgcc cctgtcgtcg aatcccacga ctgcgaaccg tgcgaggacc tcctcgacga
     6181 acccggcgat cccgcccgga tgatacgcct cgagggcgtc gagctccgcg atcctctcgg
     6241 ggtcgacgcc ggcgggcttg acgcgccggg cgtaccccct cttctcctcc ttcgatcccg
     6301 ccctctcagg gatcgtgggg atcccgaggt ctcgaacctg gcggggggag aggccgatcc
     6361 gcgccatccg gaggtccggt ccgccgccgc cggggctgat tttgtctctt atcagcaagg
     6421 cgagctccct agagaaggcg aggccctccg gggagaggtc tgagaggcag agggcgaggg
     6481 tcgggcgccg gccgcttacg cctgtggcgc ggtcgacgag ccgcgcgacg gcgtccgccg
     6541 tcggcctccc ctcgacggag acgagggcga cggacctctt caggcagacg gggaggacga
     6601 ggcgggcgag gccgggcccc gccatccaga gctcgatatt cggcctctcg atgccagcgg
     6661 ccccggcccc atccccgccc ccttcctcgg atctatcctg gaaggcgggg gggaggaact
     6721 cgccgacgag gggaggaagg atcgcgtcct gggggaggcg gccgaagagc ttagccctcg
     6781 ccacccactt atcgagcttg gggcagtcgg tctcgtggta ccttctcacc gctctgatct
     6841 cgccgacgaa gccgccgcag atggggtagt cggccgtctc tcgccccagg gcgaagtaat
     6901 agagagcggc gacggtggtc ctcggccgcc cgaactcccg gtggactccc gccacccagc
     6961 ccgtcttcgt cctgtccacc ggggtttcga ggggccttcc cgcgatctcc ttcataagcc
     7021 tccggataaa ggcggtccgg ggatttcagc ctggcgacgg ctcatcctcc caccttctca
     7081 agcccgccgt ttcatcttct gcacaagggt tttagccgaa gagctccatc catccagcat
     7141 aagcacttcg gaagctgaaa agatgtccaa ctccagggca gattcaccag gggatgactc
     7201 cgaccccttc ctcgccgaga tccggaggct ccgggggggg cgggaggcgg aggagggaga
     7261 ggactgggtc gagcgggcgg tggacctgaa ggtagaccag ctcctggacc tctccgagtg
     7321 cctgaggagc ctcgacccct cccaggagct gatcgatata agaaatgagt gcgacgggat
     7381 cctggaggag atggtcaccg ggaggctcgc cggcgaggcg gcggtgggga ggacggacga
     7441 ttttctctgc aagaccgcct ccgtcatcat ggagcggctc ctcggcgaga tcgaagagga
     7501 accgtgagat ccggatcgag ggtcacgccg aaagcccggc cgccgatccc gccatcatag
     7561 ctcccttgcc cggatctgga gctcctcggg gaaggtccag ggggggctga gcccccagcc
     7621 cctctcgtcg aggtccggag ccgcctccag aagttttcgg gtgtaggggt ggacgggcgc
     7681 cgtcgtcacc tcgcccgccg gcccctcctc gacgatcttc cctcccagca tcaccgccaa
     7741 cctgtcggag accttcctcg ccagggctat gtcgtgggtt atgaagagga tggcgaggcc
     7801 cctctcctcc tggatgttga ggaggagctt caggatcttc gcctggacgg aggggtcgag
     7861 ggccgaggtc ggctcatcgg ctatgaggag cttggggttg aggacgaggg ctcttgcgat
     7921 cgccaccctc tgcccctcgc cgccgctgat ctggtggggg tacttcttca ggaaggcgtc
     7981 ctcggagggg agctccacct cctccagaac ctttttggca atcttcatcc tctccgcggc
     8041 gcctccgacc ttctggacga ggaggggctc ggtgaccgcc tcgaggaccg tcatcctgtg
     8101 gctgatcgac tccttcgggt tctggtatat aatctggacc ttctgataga aatccctccc
     8161 cctccggagg aggggctccc cctccagccg gacctccccc tcggagaggt cgaagaggcc
     8221 gatgatggtc ttcgccagag tcgtcttccc cgagccgctc tcgccgacga gggcgatcgt
     8281 ctccccctcg tagagggtga ggtcgacgcc gtcgacggcc gccaggcccc cgaacctggt
     8341 caccgccccc gagatctcca ggagggggac gatcccgccc cggtggcagg cgatctgccg
     8401 cccgcccgcc tccaccaggg gggggaccac ctctgagcag atctctatcc tctgggtgca
     8461 ccgatcgctg aaggggcagc cgggtacgcc gtggaccatc ctccccggga tcccctgaag
     8521 gtcttttgtg gtggtcatgt tgggggagga cctgatcagc ccccgggtgt aggggtggcg
     8581 gggggagccc agaaggtcct gggtatcgcc tagctcgacg atccggccgg cgtagaggac
     8641 cgccatctta tccgtcagcc tcgcggcggt ggagatgtcg tgggttatga cgacggcgat
     8701 cttatcctcc atcctctctt tgaggaggcg ggtgatctcg gccttggtga cggcgtcgag
     8761 ggaggccgtc ggctcgtcga ggatcaggac ctgagggtcg tttgcgagag ccatggcgat
     8821 gagggccctc tgcttctctc ccccgc
//
LOCUS       DASM01000135           17281 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_77264, whole genome shotgun sequence.
ACCESSION   DASM01000135 DASM01000000
VERSION     DASM01000135.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 17281)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 17281)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17281
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_77264"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         3134 a         5420 c         5239 g         3488 t
ORIGIN      
        1 ttcagggtcc gcacctcttc gccccgctcc aggtcccata ccttgagtgt ctgatcatag
       61 gaagccgata ccgcttttcg accgtcgggg gtcaccgcca ccgcactgac cggaccggca
      121 tgacctttca gggtgcggag caagggtcct cccggcggcg tcaggctggg gataaggggg
      181 cgtatccagg attcgtctcc accccctctg atgtggtaga gcatggcctg tatcgagaga
      241 gacgataacg ccatcaagcg gcccagcacc tgaccggcga gctgagcctt gtcgccggct
      301 atgacgtgag cagagagccg gagagcatct cgcaccaaaa acgcgtcaga atcttcaggg
      361 agaagagaat cgtagtccgc aaccagagaa gggacgtctg tggctccgag cttggctttc
      421 atccaatcga aatcgaggag aagagttcgc agttcatctt tgcgcccagc ttttatgaga
      481 tggctggcca aatgctggaa gaagtagcca tcatccgggc cggtgtgcca gccgtcacga
      541 catttctggc ggtaagcttc cagcaggcgg ttgtgcagct cggtgaggtc ttcgacctgt
      601 tttctcaggt agtcgaactg gaggtcgtga aggctcaagc gtccgtcacg gtcacgccgg
      661 gctaaagatc gattgaccag aaggtccagc acttctttcg tatcatattc atccagacct
      721 tcgggcttcc agaaggtttc caagacggct tccgggatgg gcgtatcttc cgggaagacc
      781 gcaaagtcga ggtatcgaga ctggacatcc ggctccagcg catcgacgct gacttggacc
      841 gctcccatca ggtcaggata agagcagttc gggaattggc gccggatctt atccagatcg
      901 gcgttctgga gcctgtgcag ggcgaggccc caacgatcct gcctgttgtg caccatggcg
      961 ccgatcatcg ccagggtcaa tgggaggtta ccgcacttct ccaccacctc atgagcctcg
     1021 ggcgggagga agccctcatc ctggccggac cagtccacca aaagcttcag agcctcgtca
     1081 tcttccaaaa cgtccaaaca gtattcttcg gctccaagtc ctgtgattat gtcggagttt
     1141 ctggtagtga tcagcatcct gcaatctggc cccaggcggt tgaaggcctc gacatgctcg
     1201 gaattccaga cgtcgtccag gatgatcagg caggacttat tggcgagaag atcgctcagc
     1261 cgggttcgtc cctgctgcac gtcgttgaag acaacttttc catagccaag cgattcggcg
     1321 aggtccgact ggcgctgggc gagcttcggc tcctggccga gggtgatcca gatcacgccg
     1381 tccggaaaag cctggcggac ctcatcgtcc cgcgccacag ctgctgccag gaccgacttt
     1441 ccgatcccgc ccatgccctg gacgccgacc tttctggagg ctccggtgat cccggccgtc
     1501 ttcttgtcct cggatagaac cagatttttg attttcctga agtccctggc acgtggcagg
     1561 aaattttgcg gcagctccgg cacgtcgaca agcctgccga tttcaccatc ggaaccgcca
     1621 tctttgcagc ccagattggc cagcacctcg aaggcgaagg cgctggcgtt tttagcagcg
     1681 acctcctgcc acccagcttc gtcagagtct ctctttccag cgatcaggtc agagatgcca
     1741 cgaaccacca gggcgtcgac accagggttc gcataggccg ccctcagaaa gccgtagtcc
     1801 tccatcgcca cggccagaga atcgccatag tttgagcgga ggaacctgta aatggccgaa
     1861 cggttggatg ctatcacctt ctcgcccgcg gctattggac cgacgaagct ctggggcatt
     1921 tgaccaggat tgcagttctc aagcctgtcg gaccaatctt tgctccttgt caccgctctg
     1981 gctctctgtt ccatgcggaa tccggaatgc agtaattcag gccttggcag aaagatgggt
     2041 ccttcatcgg aatcgtcaac cttgccagac tcataagcat aaaccttggt tgcggcgacc
     2101 acgtcgccca ggttgacgtc cttgatccca ccagctacgc caaccaggag aaccacctct
     2161 ggtttgaaat actctatagc ccgctcggtc tcttgagcag cagtcggatt gcccatccca
     2221 atctcggcta ccaggacttc ccaagatcct tcctttgccg ggaaggttcc catgttgtag
     2281 acatttcctc ggtgatgtgt aatctctctc aggtccttca gatgtgcgca aaccgattca
     2341 aattcaatgg caagtgcggt tataacgaca gctcggcaca tttttctcct ggtccgatcc
     2401 tgctccacct ctgctagtct ggaagcactt ggaaataaag gttgtgaact ataataagca
     2461 tcgggacaga atgtccttta tttgccaact ggatcgacgg attcctaacc caaaatctat
     2521 cacttctgga tgtctggagt ggggcaccca gtccattccc ccagcctcgc ctgcttcgcg
     2581 ccgccctcct cgcccacctc cggcaccgag acccccagcc tatcctcgaa ccactccgcc
     2641 accagccgcc ggtggcagaa ggcgcccgaa ttctcccagc agaggaggac ggcgtcgtcg
     2701 ccgaggtcgg catagactct gacgggatcg agcttcgaga ggacctccgc gcggtagcgc
     2761 cggatgtact cgtcctcgtc gatggcgccg ctcttcgcct cctgcaccag gcgccaggag
     2821 ggggcgagcg gctcgtatac gcggccggtc cagccgcggg gctgggtccg ggagatggcg
     2881 acggccctgg ggtgggagcc ggagcgggcg aagctggagg tctgcatatc catctctgac
     2941 atgttttcag gctgataaag aaccgcttcc aagacgatct cactcattca gctgcgattc
     3001 cgatcgggca ggaaacggag actggggttc atgagatctt gcctcctttt ggtcagcctt
     3061 ttgctttcgc agagaagatt tatattcaca aaagcgaaaa taacctccga tgaatagcct
     3121 tcgcgactac accatcgtcc tccgtccgga cgacaacggc accttcgtcg cccgcgtccc
     3181 cgccatcccg ggatgccacg cctgggggag gactcccgaa gaggcagagg cagagattgc
     3241 caacgtcttc gagatgataa aagaggaata ccgagaggag ggaaagccct tcccctgatg
     3301 gctctaaatc taaaatgatc acgataaaag gatggaaaaa aaaggtaaaa aacggtggtg
     3361 gcgcctttag cgccttcagc agcggcctag ttgatccgcg ccaccgccgc cacctcgtcc
     3421 cccgccttca gccgcatgat ccggaccccc tgggtcgccc ggccctgaat tcggatgtcg
     3481 gaggcgggga tcctgatcat caccccgtcc ttggtggtga tcatcagctc gtcctcctcg
     3541 gagacggtcc gggaggcgac gacgtccccc ttcctggagt cgatgttctt catccccttt
     3601 ccgccccggc ggtggggcga gtactcggag agctccgtcc tcttcccgta gccctgcctg
     3661 gtcacggtca ggagagatga cccgggccgg accacgtcca tgcagatcac cttgtcctcg
     3721 ccttcgaggg ttatgccccg gacccccatc gatccccggt tgctagggcg gacgtcagcc
     3781 tcggagaacc tgatcgcctt gccgccggcg gtggccagga tcaggtcctg gtcgcctccc
     3841 gtgagccggg ccgcgaccag gctatcgccc cggaggttca cggccttgat cccgcccttc
     3901 cgggggttgc tgtaggcccg gatgggggtc ttgaccacct gtcccaggga cgtggccatg
     3961 acgatgaacc cctcggagtc gaagctcttg accgggatcg cctcggtgat cttctcgtcc
     4021 gcctccaggg agaggaggtt tacgatcgat tttccccggg cggttctgcc gaaagatggg
     4081 acgtcgaaga cccggagcca gtgggccttg cccatatcgg tgaagaagag gatgtactcg
     4141 tgggtggtgg cgatgaagag gtcggtgacg aagtcctcgc tcttggtgtc ggcccccatg
     4201 acccccatcc caccccgcct ctgctggcgg taggcggaga ggggctgcct cttgatgtag
     4261 ccgtcgctgg tgatggtcac cgccacctcc tcctcctgga tcaggtcctc aaggtcgatc
     4321 tccccctcgg cctcgatgat ctgggtgcgc cggccgtcgc cgtactttcc gacgagctct
     4381 gccagctcct gctttatgat gttgaggatc tcccgggggt cggcgaggat ctcccggaac
     4441 cgggcgatcg ccacctccag atccatctcc tcggaccgga tcttcccctg ctcgagagcg
     4501 gtcagcctct gaagcctcat atccaggatc gccttcgtct gctcctcgga gaggccgaag
     4561 gcctcggtga gggcggccct ggcctcggag ggcgactcgg cggcgcggat gagcttgatc
     4621 acctcatcga tcttccccag ggctatgagg agccccgcca ggatgtgggc cctcttctcc
     4681 gcccggtcga ggtcgtagct ggtcctcctc tcgatcacct cggcccggtg agatacgtac
     4741 tgattcagga tatcccggag ggggagggtc gccggctggc cgtcgacgag gacgacgttg
     4801 atgatgctgt aagaggtctc cagctgggtc tgccggtgga gctggttgat gaccacctcg
     4861 gggttgaccc cggaccggag ctccacgacg agccggatcc cctcccggtc cgactcatcc
     4921 cggaggtcgg agatcccgtc gacccggtcc gccttgatca ggtcggcgat cgtctcgacg
     4981 accttcgcct tgttcacctg gtagggcagc tccgtcacca cgatcttcgg ccgctcgccc
     5041 ctctcgatca ccgacttggc ccggagcttg attatccctc ggccggtggc gtaggcggac
     5101 tcgatcccgg aacttcccag gatgtagccc cccgtcggaa agtcggggcc gggaagctcc
     5161 tccatcaggt cctctaaagt cgaatcggga tcatcgatca gcctcgtaat cgcccccgcc
     5221 acctcgccca agttgtgggg cgggacgttc gtcgccatcc ccacggcgat cccggaggag
     5281 ccgttgacga ggaggttggg gagcttcgag gggaggaccg tcggctcctt gagggagtcg
     5341 tcgtagttgg ggacgaagtc gacggtatcc ttctcgagat ccgccagcat ctccccggcg
     5401 atctctgaga ggcggacctc ggtgtacctc atcgccgccg ccggatcgcc gtcgatggat
     5461 ccgaagttcc cctggccgtc gatgagggga tacctcatcg agaagtcctg ggccatcctc
     5521 accatcgtct cgtagatggc ggcgtcgccg tgggggtggt acttacccat cacgtctccg
     5581 acgaccctcg ccgacttctt gtagggctga tcgtgggtca tcccctgctc gtacatggcg
     5641 tagaggatcc ggcggtggac gggcttgagg ccgtcccgga cgtccggcag cgcccgtccc
     5701 acaatgacgc tcatggcgta atcgatgtat gaggacttca tctccgaagt tatgtcgacg
     5761 tctatctccg tcatcttctc acctcagacg tccaggttcc tcacgtcccg ggcgtgggcc
     5821 tcgatgaact cccgccgggg ctccaccttc tctcccatga gtatcgaaaa gatctcgtcc
     5881 gcctccatgg cgtcctccag ggtcaccttc agcatcgtcc tcctctcggg gtccatcgtc
     5941 gtctcccaga gctgctccgg gttcatctcc ccgaggccct tgtacctctg gaccgtcgcc
     6001 ttccccccgg acccctccag gattccggcc agatccgctt cggagtaggc gtaggtcgcg
     6061 gccttacccc tcttcacctg gtagagggga ggctgggcga tgtagacgta gcccgcctcg
     6121 acgagctccc gcatgtaccg gtagaagaag gtgaggagga gggtccggat gtgggagccg
     6181 tcgacgtcgg cgtccgtcat gatcaccacc ttccggtacc tagcccggtc cagctggaag
     6241 tcgtccccga tcccggtgcc gagggcggtg atgatgtttc ggatctcgtt gttcttcagg
     6301 atcttgtcca gcctcgcccg ctcgacgttg aggatcttac cccggagggg gaggatcgcc
     6361 tgaaagtgcc ggttcctccc ctgcttcgcc gagcccccgg ccgactcgcc ctcgacgatg
     6421 tatagctccg atagctgggg gtccttctcg gcgcagtcgg cgagctttcc gggtagccct
     6481 cccgagctga gggcgttctt cctccgggtc agctccctcg ccttccgggc cgcgacccgc
     6541 gcccggtagg cctgggtcgc cttctcgacg atcttcaccg tcaccagggg gttctcctcg
     6601 aagaactcgg acagcccctc ggagacgagg gagctgacga tccccccgac ggcgctgttc
     6661 ccgagcttcg tcttcgtctg cccctcgaac tgggggtcgg cgagcctgac gcttatgacg
     6721 gcgacgagcc cctcccggag gtcgtcgccg gtgagcttgg tatcgtcctt gaggagcttg
     6781 ttcgccctgg catactcgtt cgtcgtcttg gtgagggcgg cccggaagcc ggcgaggtgg
     6841 gttcccccct cccgggtgtt gatgttgttg gcgaaggaga ggacgttctc gttgtaggtg
     6901 ttgttgtact ggagggccac ctcgacggag gttccgtcct tctcccggtt gaagtagatc
     6961 ggcggggagt ggaggatgtc cttgttcttg ttgaggtgct cgacgaagga gacgataccc
     7021 ccgtcgtaga ggtactccct cctcttggcc gtcctctcat cctcgacgga tatcttcagg
     7081 ccgcggttta agaaggccag ctcccggagg cggctagcca gggtatcgaa gctgaactcg
     7141 acggcctcaa agatctcggg gtcgggcttg aaccggacgg tcgtcccggt gtggtcggag
     7201 acgccgtgga cctccaggtc cgagacgggc ctcccccgct cgtacctctg ccagtagacg
     7261 aggttgttcc tccggacctc cacctccatc cactccgata gggcgttgac gacggagacg
     7321 ccgacgccgt ggaggccgcc ggagaccttg taggacttgt ggtcgaactt tcccccggcg
     7381 tggagcttcg tcatcactat ctccagcgcc ggcttcttgt aatgctcgtg gatgtctacg
     7441 gggatgcccc ggccgtcgtc cctcaccgag gcggtgccgt cggcgtggag gacgaggttg
     7501 atctctgagc accggccggc catggcttcg tctatgctgt tgtccaccac ctcgtagacg
     7561 agatggtgga ggccggccag gttggtgctc cctatgtaca tggcgggcct tcgcctgacg
     7621 gcgtcgagcc cctccaggac ctggatggtg ctcgcgtcgt actcctcaac tacttcgctc
     7681 aagaccggat tcgcccccga taacataaat gacccacaat tgaattatcg cattatatat
     7741 aacggttttc ccgaattaat cgcaagccgg cagctgcgaa attttgattt gatatatttt
     7801 attcgaagac ttcatcccgc ccagggggga tctcccatcc ccgaggcttt tgcgggaaag
     7861 gagggataga cctttggcta cttagcctta acgtgcccct cccctccagc cccagggggg
     7921 cggcggtccc cggcggcaag cggacttccc gggccggggc aggagaatgg aaggtgccgg
     7981 gagccggccg ggtggggggc tatacggagc cctcgacggg gacgaacttc gtcccgtagt
     8041 agattatccc ccggtccccg tctttgccga ggatcctgaa gacgggcttc accctcatcc
     8101 cgatctcgat ctcctcgggg gcgcagatca cctggcccgt cagcctcggc ccctcctcca
     8161 gctctatgat ggcgaggttg tagggggtct gcctcttgaa gtcgtcggtg gcgttgtaga
     8221 tggtggtgta ggtgaccacc gtccctcggc catcgaactt gtaatcctcc atcttggcgg
     8281 ctctccggca gttggggcag atcgtcctcg gcgggtagta atactcgtcg cacttgggac
     8341 agtgggtccc caccaggttg tacctctggg gtatgtgtct ccagaaccgt gggactgtgg
     8401 acaattggtc acctcgacat gatgtggacg atggcggtgc cgccgcaccc gccgacgttg
     8461 tgggtgaggc cgacatcggc gccctcgacc tgccgcactc ccgcctcgcc ccggagctgg
     8521 aggaccatct cgtaggcctg ctttatcccc gtggcgccga cgggatggcc gcaggccttc
     8581 agccctccgg aggtgttgat ggggatctcc ccgccgatgg cggtgagccc ctcccgggtg
     8641 gctctccccc catcgccttt atcgaagaac cccagatcct cgatggccac gatctccccg
     8701 atggtgaagg cgtcgtggac ctcggcgagg tccacgtcct ccggctccag ccccgccatg
     8761 cggaaggccc tcctggcggc gaagaccgag gcgtcgaggg tggtgatatc ccgccggtcg
     8821 tggagggcga gggtgtcgct ggcctgggcc gtcgccagga tcctgatggg gctgtcggtg
     8881 tactttttag cctcctccgt cggggcgagg acgacggcgg cagccccgtc ggtgatcggc
     8941 gagcagtcca ggacccggag ggggtcggcg acgagggggg agttcaagac cttatcgacg
     9001 gtgatctcca tctggtactg agatatgggg ttcatgctcc cgtggtgatg gttcttcacc
     9061 gccaccgccg ccaggtcctc ccgggtcgtg gcgtaccggt gcatgtggag cctcgcgatc
     9121 atggcgtaga gggcggggaa ggtcgccccg aagaagcatt cccactcccg gtcggcggcg
     9181 gcggccagag cgtccgcggc gacgccaccg gtgacgtcgg tcatcttctc gaccccggcg
     9241 gcgatgacga cgtcggagta gccgctcgcc accgccagga ccccctggcg gagggcgagc
     9301 cccccggaag cgcaggccgc ctcgaccctg gtgctgggga cgtggaggcg ggagaggccg
     9361 gcgcagtcgg atatcagggc gccgatgtgc tcctgctcca cgaaccggcc gccgctcatg
     9421 ttcccgacgt agaggccgtc gatctcctcg ccggagacgt tcccatcctc cagggccctg
     9481 atccccgtct ccaccaccag gtcccggagg gagtcgtccc acctttcccc aaaccgggtg
     9541 catccagctc cgataatcga taccgatctc atggtctcac agcctcagct ttcccttgtg
     9601 cttggcgtag acggcgtagt ccaggtagcg gacgtcctcc atgtactctc tgacccgcgg
     9661 ccctcggtcc ctaatctcct cgatcccctc ctcgaccctg atgctgaagg cgtcgctccc
     9721 cgcccccgac ccgaagctcg cgaggaatat cctctccccg gggcgggcta cgtccagggt
     9781 cgcggcgagg ccgatgaggc ttgagcccga gtaggtgttt ccgatccagg ggacgacgag
     9841 ccccggctcg atctgttcag cggagaagcc gagcttcgag gcgaccctcg cgggaaactt
     9901 cccgttcggc tggtggaaga cggcgtaatc gtaatcctcg gggatcgtcc ccatcttctt
     9961 gaagaggccc cgggtggcgc tggtgacgtg cttgaagtag cccggctccc ccgtgaacct
    10021 acccccgtgc tccgggtagg ccatcccctc ccgcctccag aagtcggggg tgtcggtggt
    10081 gaaggagtag aacccctcga tctccgccgc caggttctcg cagcctatga cgtacgcggc
    10141 ggcgccagcg gcggcggtgt actccagggc gtcgccgggg gcgccctggc ttgtgtcggt
    10201 gccgatggca aggcccagat cgatcagctt tcccgccacc aggccgaggc aggcctggac
    10261 cgccgccgtc cccgccttgc aggcgaactc gaagtccgct gccgtcagct ggtggtcgac
    10321 tcccagggcc tcgccgacga tcgtccccgt cggctttacg gcgtaggggt ggctctcgga
    10381 gcccgcgtag atcgccccga tcctggcggg gttgatggag gcccgcttga cggcgttcct
    10441 cgccgcctcc accgccatcg tcaccgcgtc ctcatcggag tcggggacgg atttctcgaa
    10501 gacgttcagc cccttggcca tgtcctcccg ctctccccag accctggcga tctcctcggc
    10561 ctttatcctg tacctgggca catagacccc atagctgact atccccgcgg gcaaagcatc
    10621 acctgttgtt cagattctcg gtcagatcct cggtggagag ggtcgtcgcg aggaggggta
    10681 tcctctccag ctccgcgatc ctcctggcga cgccgtccac ccccgagggg tcgatcccct
    10741 ggaggatcac ggcgccgggc ttgaggtggc ttaccctcac cgccaccatc ggcgacctgc
    10801 cggtggagac gccggtgaag atcagggccc gggcggtgct ccggccgtag atcctgtgaa
    10861 actgttcggg aagaagctcc aaaatagcct tcaagctgtc gatgatcgta tagccgtaga
    10921 tagggacgtc gacgtccccg cagacgacgc aggcgccgat ctggtctacg aacttggaga
    10981 ggggtatcgg ctcggagtat tcgcatatgc agcatattcg cccgaaggaa ccgccgtcgc
    11041 tgagcagagc gctgtaggtc cgggcggtgc agctcccccg gagctcgtcg atggtgagga
    11101 gggcatcgat gatcttgctg acgatcagta tgccggggga ccttcgtctt ccgctttcgt
    11161 agtcgcttat caccgatggc gagatcccca gatggttgga gagctcggtc tgggttacgc
    11221 cgaagttcct ccgccacttc ttcagggtct cgccgggatt ctgtgataaa gtgatctcac
    11281 cagccatctt ctctgcgagc agtcctcgaa catcggagat atcccccccg gcagacatga
    11341 gccagagagg gggctcttta gaatataaag ttaacgaaga ggagttcgtc atctgacgtt
    11401 ataaggtgac tcgccccaaa acaagatgcg ccgaccggga ttcgaacccg ggttttgggc
    11461 ttgctccgcg acccctgccg cttggaaggc ccaagtcata accactagac catcggcgct
    11521 caccacctcc atctcctcgt cgaacttaac gttttcgatc cgagggtgga gcgcccccgg
    11581 gccggggcga tcccccaaag gatggcaggg aacgggccga cccccgcccg ccgggagagg
    11641 tcttggcggc gccacccgga tctcccccat gcccgatagg cagctacgcc tcggatacct
    11701 tgatatatcg aggcgtcggt ggagtctcgc cgttaggtga cctccggagg gaacgagatc
    11761 ttgcattcgg tgataatcgc gatctggctg atgctgccgg cctacctgcc gaacaacttc
    11821 gccgtcatct tcggcggggg gaggcccctc gacttcggga ggaccttccc ggacggccgg
    11881 cggatcctcg gcgacgggaa gacctttcgg ggaacgatcg ccggcaccct ggcgggggtg
    11941 ctcttgggcc tcctcctcca cctcatcgcg gccgcgaggc ctgatctcgg cctcccgggc
    12001 ttcggccgcg gattcgagct gatcctcgtc ctcgtcggcc tctctctggg ggcgatggtc
    12061 ggggacatcg tcgccagctt cttcaagcgg aggatgggga tggagcgggg ggcggcgctc
    12121 ttcctcgtcg accagctcga cttcgtcttc gggagctggg ccctcaccct cctcctcgcc
    12181 ccccggtggt tctccgagag cttcactgcc gaggtgatat tcatcgtcct ggtcgtcacc
    12241 cccgtcctcc acagggtgat gaacgtcctc ggctacagga tcaaggcgaa gaaggagccg
    12301 tggtgaggga gatagagggc atcttccgga cgatctggga tttatatcga tcattccgag
    12361 ttagatgggc catatcgcca ttattcctac atatttcagg aataattcgc cccaaattgt
    12421 tcttccttgg tttttttata tttcaggctt ttatttgggg atttcagatc tttcgggctc
    12481 actaaaaccg ggacaaaaaa caaaaatggg gctttcattt ccatttcgaa agctttataa
    12541 gtccagtaac cgatagtcag tacgttactg gccaaatttt tcatagatta ctatgatttg
    12601 gcaagaatta aaaaggagga gagcaaatgg acatggttag catggggctc ctagcagcta
    12661 tgggcgccct tgcgacagta gccggtgcaa gcgaggactt agagtccgat gtgggttctc
    12721 agagcaaccc taactcacag gtccagttgg ctgcccaggt gggtcacacc cacaggatat
    12781 acaacaaagc catctctgga gagccaccat cgaacgctct ttacgccgcc cttgccgcag
    12841 ttacagctag tgtcttgatg tccgggtatg gcatgaacgc gttctttgcg atcgccatcg
    12901 gctcggttat ggccgcgatc ttcaacggca tcttttctgt caccgcctac atgggcagga
    12961 acacgagcca ggcgcggttc aaccagtacg tctacctgga cgttctgcgg actcacacca
    13021 ccgccatcat ggcacatgcg tacatcactg ccttctgcgt agtcaccatc tcctacatca
    13081 tgacagctcc gtggcttccg attcagcacc cgttcccgtt ccccctcgtc gccatgattt
    13141 gggggctcgc catcggagcc ataggatcgt caacaggaga cgtccactat ggaggagaga
    13201 gggagtttca gaactacatc ttcggggcgg ggctcaacgc cgccaactcc ggtgacatcg
    13261 tcaccaaggc cgaggctggc ctgaggagct ccatcgacaa cgtctggttc tgcgccaggt
    13321 tcggaggccc cgtcaccggg atggccttcg ggctgacggt cttcctggac aactggcgga
    13381 ccaccatctt cgatcccgcc acccagggat ggtatgcggt cctgaccggg atagtgatcg
    13441 tcattctgtt catcgtctac aacagaatcc tagagatcag agcgagaaag aggtacgggc
    13501 cctatcccga atacaagggg gacattgagg catgattcct ccgattgacg ctaatatgct
    13561 agtgcacata attgagatcg tcattggcgg gcttttggtg ggcataggcg tccacttcgt
    13621 ccccgtcggt ggcgcaccag ccgccatggc ccaggccacc ggggtgggga ccgggactgt
    13681 gcagctggca gccggctctg gcctcaccgg tctattgagc gccggcctga tgttgaacta
    13741 caccgacagc attctcctgg tggttatcag cggtgccgtc ggcgccatga tcatgatcga
    13801 cgccaccatg atcgtcggaa actgggtcta catctacggc gtcgggacgc ccccggcatc
    13861 cgccaaggcg aactacgacc cgatcaccaa agatccccag gcgccctacg tcgcccccgg
    13921 gaccgtcggg caggggatac ccaccgtctg cttcgtcagc gggaccatag gaggtctcct
    13981 cggcggcgcc ggtggatccc tgatctacta cgtcctctat cagatcaacc gggaccccgg
    14041 cctctcggcc ttcttcgcca tcgggatctt cgtggtgaac gccgtcatcg cctcctacaa
    14101 catccagggg acgatcgagg ggttccacga ccccaagttc aagcgggttc cgcgggcctt
    14161 ccaggtctgt ctggtggcat cgctgatcat tgcaatattc gccgtcctga tttcgggagg
    14221 tcactgatat gagcgttgca gcaggtggag gagcatccaa gatcgacaag acgaagctga
    14281 tgatgtacgg cctcggcggc ggagtcctcg ggatatacct ggcccacgtc ctaaacgagg
    14341 tcaccgggac cgattacttc tcgttcctgg cggggctcgg cgtcatcgcc gccattgtga
    14401 tgggggctga cgccgtcagg agggtctgca gctacggcat cggcacggga gttccgtcca
    14461 tcggcctgat ggccatgggg atggggctcg tcgcggcgat gttcggccta tccataggag
    14521 gcgtcgccgg acccatcata gggatcgccg tctccatggg cttcggatac gtcgtcggcc
    14581 ttctcactaa caagatcatc aagatgaaca tccccgtcct ggaggagact ctgatgaccc
    14641 tgggcggcgc cggggcgata gtcctgatag gcctgggggt catgatctcc ggagttatag
    14701 actatagggt gatcatcgga aacgtcatcg ataccggata catcgccata gtcttcatca
    14761 tcggaagcct ggccatattg caccccttca acgccaacct cggccccgac gagacccagg
    14821 acagaaccct cgtacactcc atctccgcgg gagccctggc gatgttcgcc gtcgggatcg
    14881 cgtccctgga gaccataggc gtcgagggct tcgtctcgat attcgtggcc gccgtcgtct
    14941 ggatcgtctt cttcaagaag ttcttcgagc tggtgaagag ggactccgcc ggcgtcaagg
    15001 ggacgggtct tcttcctgag ggaggtatgt agatgggaat agtcagagct tctccctccg
    15061 atctgcacct ggtcttcgat cccctggagg ggatgatcgc agaggagagg gacgacgtaa
    15121 tccagttcgc tctggacccg atcgtggccc agatcgatga gataaacaag gcagccgacg
    15181 acctggtcaa ctccctcgac ccccgggcgc cgctcctcaa ctcctacaag gggagggagg
    15241 gggcgagcta catggcgggg atctacacca acatctggta cgggttcatc atcggcctcg
    15301 tcgtagcctt cgtgtactta ctctcccatg ggggtgtctt ctgaatggcc aacaagaagc
    15361 cgacatctga accctggccc tggctcaccg gcgagtacgc ggtgggaaac cccgagagcc
    15421 cggtggctgt ggtgacctgt gggtctcacc tgaacaacgc gcctttcctc gatgcgggag
    15481 cagccctcgc aggcccctgc aagaccgaga acatcggcat cgagaagatg gtggccaaca
    15541 tcatctccaa ccccaacatc cggtttctga tcataaccgg gatggaggtc aagggccaca
    15601 tcacggggca ggcgatcgag gccttcgccg ccaacggcat cgatcaggaa gggaggatcg
    15661 tgggggcgac gggcgccatc cccttcatcc agaacctgac cccagaggcg attgagaggt
    15721 tcaagcagca gatatcgacg atcaacatca tcgacaccga ggataccggc accatcgtct
    15781 ccaagatcaa ggagtgcatc tccaaagatc cgggcgccct agacgtcgag cccatggcgg
    15841 tggagatatc cgagggcgaa gcggaggagg aggcccacgg ccttcgtccc atggcggggg
    15901 agatcgccaa catccgggca cggatgaggc agatcgacgc cgcagtcatg aacatgggga
    15961 tccagaacaa gtactctgcc ggcatctacg ccggaaagat cgaggggatc gcagcgggcc
    16021 tcaccatcgt tttggtcctc ttcggactga tcctcagcat cggagggatg tgagatggca
    16081 gatgaagaga ttgtgagcgg agagggcgca ggagcaggga aaccgataac tgccgacatc
    16141 ccatacggaa aggggcttcc cacggtgctg gcccccttca tggggccctt cgaccagatc
    16201 accgacagcg tcctgtacag ggcgcagctg gtggtcagag atcagaagct cagctcgggg
    16261 gtaaacgccg cgatgccgat cggagcggtc gtcgggttcc tcttcgcgat cctgatggtc
    16321 ctcttgccag tgtacattat gacctgaggt gatacattca tggctcagaa caatcgagac
    16381 gtagtaccca gcgtcgtagt agacccggac cagtacaggg aggtcctgga caagctggcc
    16441 aagatcgacg agaagatcga gttcgtcagc gcggagatag cccagaggca gggcaagaag
    16501 ctcggcagag atataggcat cctttacggg gtctgtggag gattaatatt agtgctggca
    16561 tactatttgc tggcggttgc gtgagggttt gatatgttca ggtttgacaa gaaacaagag
    16621 gtattcgagt tcggcaagat caaggtgggt ggtcagcccg gcgagtaccc caccgtgtgc
    16681 attgggacga tgttctactc ccggcacaag atagtgacca acgaggagaa gggtatcttc
    16741 gatgtggctg ccgcggagaa gctctggaag gaccaggaag agatgagcga caccaccggg
    16801 aacccctgct tcaaccagat cgtcgccgag acgaacgaag ccatgaccaa gtacctggac
    16861 tggttcgtcg agatatccga cgcccccttc ctggtggact ccagcgcccc gagcgtgcgg
    16921 ggcttcgggg tcaagtacgc caccgagatc ggcgtcgccg accgggcgat ccacaactcc
    16981 atcaacgcca gcatcacccc tgaggagttc caggccgtca aggagagcga cctcgacgcc
    17041 gccatagtcc tcgctttcaa cgccctcgac tccaccacca aggggaagat ggacgtcctc
    17101 accaaggctg cgggaggcgc ggagaagggg atgctcgact acgccgccga ggcggggatc
    17161 accaggcccc tcatcgacgt cgccgccatg cccctgggga acggctccgg cgccaccatc
    17221 cgggctggga tcgccgtcaa ggccctcctg gggctccccg tcggggcagg ctaccacaac
    17281 g
//
LOCUS       DASM01000136            7194 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_107452, whole genome shotgun sequence.
ACCESSION   DASM01000136 DASM01000000
VERSION     DASM01000136.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7194)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_107452"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1275 a         2249 c         2575 g         1093 t
ORIGIN      
        1 agaccggggt tttacgcccc tctccactcc ccttttttat aaaatcgggg actcctcaga
       61 ccccgatatc agggttgatc cgatgggggc cggaatagat catccccgga cctatccctc
      121 gacccggggc ggatcgtcgg cggaatttcg gtgccgtatt catcccgcca tgccgcggac
      181 ccaagctgat ggtgcccccc catcccccgg cccgcggcgg ggctgaggat cacggcgtag
      241 ctcccggcgt cagctccgcc gaagatgacg agcctcccga cccggatctc cgacccctcc
      301 cggcggatgg tttcgataga ggcccgcccc gccgcctcca gccctccccc cggggcgggg
      361 gggatcgaac cccacccccc gacggggacc atcaggtccg cctccagggt ggtatttcca
      421 ccctcggcgg tgaggttgat tcggtccagg aggtaggatg agcggccgag gcggaggttt
      481 ccacgatagg cgtattcccc ctccgccccc tcgatctccg ccctaatctc ccccagggtc
      541 cggttctccc cgaggagcct ccggattcgg gagggatcga cgcccctcac cctctccacc
      601 tgaagcctca gggccaagac ctcctccttg tcccccaggg cgaaccccac ctctgagagg
      661 actatggaga tcagcccttt gggacaatcc atcggagggg ggaggtcgga gaggacgggg
      721 caggcccccc caaacccccc gccgccgggg atgggatggc cggaccccag ggaggcgaag
      781 aggatcagag ccaccatccc caccaggagg ccccatggga acgggtgcct catcctgcga
      841 tcccccccag cccctgaggg agacgcccct ggggcaccag ccagagcctc ggggacggcc
      901 gcggtaccgc cgggatgaag atccgcctca tctccctcca ccccagttga gccgctccga
      961 cagctcgatg acgttcgtcc ttccccgttc cttcttggtc acgatattcc gcctctccag
     1021 ggagacgatg atcccggtga gggaggattt ggggatcccc gtctcgtacc tgatatcggc
     1081 ctgggtcatc ttcccctcgt tcttcatcag cgcctccagg acagacctct ccctatcggt
     1141 gagggtatcc atcaccgccg ccatctcccc cgagatccga gacctccccg ggccggcagg
     1201 ggatgatcct gggtccaggg gctcggtggg ggctgagact gccggaggcg ccccggacgc
     1261 ctcctctgcc ccggccccgg cggaggcctc ccccggcggg aggggacggg gcttacccca
     1321 cccccccagg ctcgcggctc cgaggacggc gaggacgacg atcaccgccc ctgccaggag
     1381 gaggcggggc ctggaggtcc cccccggcgg gagggcctcc cccgccgccc ccccgccgcc
     1441 gacgggctgg cggtacctga tggtggcggc ggggcttgcg acgtcgtagc cgtggaactc
     1501 caccaggaag gactcgctgg aggaggagac gaggtaatcg atcccctcgg tgccggtgat
     1561 gttggtgagc tggaccttct ccgagaggta aaaggtgacg tgatactcgc cgtagctccc
     1621 tgaggcggat agcccaatct cccagtcatc ccccctcttg gcggtgaggg agttggtcac
     1681 ggcgtagagc tggcgggcct cctcatcgta ctggtattcg gaaccgttga ggaaggcgag
     1741 gccgtcgatc ctcatggcgt atcccgtcac tagggccttg ccggcgtcgt cgacgtaaac
     1801 gtccaggacg agccccgcca cctcggagcc cgcggcctcc gccgcccagg agccagagct
     1861 gaggacggct gccgcgaatg cgaggagaag cacaggccat cggcagagcc ccattatatc
     1921 cgtcatcaaa tccttcgcca accccgatcc tgatcggaat ttggtggccg tatatattta
     1981 agatttggga agatcgaaat cgccgccgaa ggccagaacg gcgcagttcc cctcaaaatc
     2041 tgaccccggg acgcataatt cataaacgat caggtctaaa tatggatgtt ctacatgacc
     2101 gggatctctc atagggtcgg ggcgggcacc aggcccctcc tcgggcgggc cttcctcctc
     2161 ctgatctcat ccgccctcgt cctcgggtcg gcggggctgg aggcgaacga atacggcgag
     2221 atcgatgagg tcccagaggc cgagccctcc ggagaggaga ccccccctgt cgcagtcgtt
     2281 gacggggaag ctccagcccc ggacccctcc gccccggagt tggagccggg tgaagcggag
     2341 ccggaggctt attccgcctc ggaggagaag accgccgagg cggtcgtcta cgtcttcaac
     2401 cgggacgacg acgccctgag catatccctc ctcatcgact cggagctggt ggggaccgag
     2461 gaggtcccca aggagaagga gaagaagttc gggagctacg agctggcggc cggaccccat
     2521 gacttcagga tcagctggtg ggacgacgac accaagaaga cccaccggga ggacctgacg
     2581 gtggaggtgg aggggacgac ggcggtcacc ctatacgcga cccagaacaa gggggcggag
     2641 aagttcgagg tcaacgtgat gttgaggaac gacaacgacc aggatctgga ggcgtacctc
     2701 tacatcgacg gcgtatacga gaagctgaag acagcgaaga agacgagcac caccgacttc
     2761 ggcaagttcg acctggagga gggggcccac gtcctggcgg tgaagtggca ggacccggcg
     2821 accaagatcg agtacgagaa gaggaagacg atccggatca gcgggaagga ggcgatcacc
     2881 ttctacgccc ccgcggggat gaccttcgag gaggtggagg cgaaggacgc cgccccgaag
     2941 gctgacgcca ccccttcccc cgcgaagacg gtgagggaga cgcccccagc ggctgaggag
     3001 gagggggcag ccagccccac cggagctgat ccgaaggcgg atgcgccaga atcccgagag
     3061 gagaggggag acgccggaag gacggaggcg accggctccc cgggacccga ccaggaaccg
     3121 acctcgagcc ccgcagggtc gaccttggtc gtatccgccg cggcggcgat cctggccatc
     3181 tacatcctct tcttcaggcg ttgattccga tgagagccct cctggcatat cttctcctgg
     3241 catcgatgat cgggacggcg gtggagtggg gggaccgggc ggagggggag ctccattggg
     3301 gagaggccct ctccatcgga ggctatagcc tggaggtggc ggacttcacc cccgaggaga
     3361 ggacgccgag gatggtgatg ctccacctcc ggaagggggg cgagctgatc gccacccgag
     3421 ccctggggcc gggggagagc ttctccttcg atgacgcggt gatggtgagg gccgaggagg
     3481 tgatgatgag agactacctc ctggacggat cggcggagcc ccgggcccgg gtcgccctca
     3541 tcctccgggc ggtccccgac ctcaccgtcc gggtagtacc gaaggaggag gcttaccggg
     3601 gaggggaccg ggcgaggctc gaggtcgagg tggagaacgt cgggatggcg gcggcggagg
     3661 aggtggagct ggaggtggcc ttcggccccg ggatcccctc ggcgaagcat tcgatatcca
     3721 gcctcgcccc gggggaggtc tgggacgggg acccttccac ccgggaggtc gagcccctga
     3781 gggtgagctt cagagggccg ccggtggcgg ggccccagga gctccgggtc gaggcgaggg
     3841 cccgttacct cgaccccgag gggggggtcc acgaggcggt gggcggtacg agcctcgaga
     3901 tcttcggccc cctccgggtc ttcaaatacg ctgaggagga ggtccggttc ggcgaggaga
     3961 gctacgtcca cctctccgtc agcaataccg gccccaggcc ccaggaggtg gtcctcaccg
     4021 actcggtggg ccgagatttc ctgacggggg ccaccctgga gtggaggatg acggtccccc
     4081 cgggggagag cgagggggcg agctacaccg tcaccgcgaa gaggccgggg gaggggcagg
     4141 ccctcccccc ggcggaggcg acctactccg tcgacgggaa ggggtaccgg gtgaggtcga
     4201 tgtcccccgt cgtcgacgtc gtcggccccc tggtggaggt ggagaagagg gcctcctcct
     4261 ccagggtcgg ggtgggggag gaggtgacgg tgacggtgac ggcgaagaat gtggggaacc
     4321 ggagggctaa ggcctccctc caggagacgg tccccccctg ggcgaccctc gtcggggggg
     4381 agacggagct ctccctcctc ctcctgccgg gggaggaggc ggcgatcgtc tataccctgt
     4441 cgtgcccgga gccggggagc ttcaccatcc cggccaccac cgtctgctac cgggacgacc
     4501 gggggaccgc ctgcaccctc gagtcctcgc ggctgaggat caccgtcgag gaagaggagg
     4561 aggccaccgc cgaggaggct cctgggccgg aagaggggcg tgatcccccc tccgccgagg
     4621 cgcagcccgc ccccggatcc ggaggggggt tcttctgggc cgtccccgcc ctgatcctga
     4681 tgatattcat agccttcgat cgatatattt agagctgaat tgaatggacg gcttggtgag
     4741 gatgggaccg gacgatcttg gatggggagg ggcgtcccca gaacgccccc ggaggcttgc
     4801 agccctggtg ggggcagctc tcctcctcct ccagcccgcc ctcggccagg cgccggacct
     4861 cctctgggag aaggccttcg gcggcccggg ggaggagagg gggatctccc tccaggagac
     4921 ggccgacggc gggatcatcg tcgcgggcta caccaactcc cgggggagcg gcggccggga
     4981 cctttggctg gtgaagaccg attcggaggg ggagctggag tgggatagga cgatcggcgg
     5041 ccccggggac gaggaggggt gggcggtcct ggaggtggag gacgggtacg tggtggcggg
     5101 ggtctcctcc tccggggaag gcggcggaag agacctcctc ctcctcaaga cctactccga
     5161 cggctacgag aggtggtcga agaccttcgg ggggccgggg gacgactggg ggatcgccct
     5221 ggtgaggacg gaggggggat acctcgtcgc cgggtccacc acctcccggg gctcgggggg
     5281 ctccgacggc tggatcctcc tcgccgaccc cgacgggaac ccggattggg agctcgccat
     5341 cggcggatcc aagaacgacg gcctctcctc gatcctggag gtggcggacg gttacgtcgc
     5401 cgtcgggacg accgagtcct acgggggggg aggcaaggac gtctggctcg tcaagacgga
     5461 ccgggagggg gggaggatct gggagaagac cttcgggaag ggcggtgacg agaggggaaa
     5521 ctccgtcctg aaggtggcgg acggctacct catcgtcggg acaacgggac ttgaggggtc
     5581 gagcgggagg gacctcctcc tcctcaagac cgacctccag ggggagaagg tctgggagga
     5641 gacgatgggg ggacccgggg aggacggggg ctggcaggcc ctggaggcgg ggggcggcct
     5701 cgtcgtcgtc ggctacaccc ggggcgccgc cgaccaggac gtctggctcc tctcggtcag
     5761 ctccgccggc gagaaggtct gggatgcgac cttcgggggg ccgggcttcg acctggggag
     5821 ggcgatcgtc ccgacgggcg acggggcgat ggcgatgacg ggctggacgg agtcccgggg
     5881 gtcggggggc caggacctct ggctcggaaa gaccgaggcg gtggattgac gggccgaccc
     5941 cccggaaggg ggagaggggc ggccccgggg gacgaggtct acgagcccgc tgaggactcc
     6001 cgcctcctcc tcgccgccgc cctggaggag gtgacccccg aggatcgggt cctggagatc
     6061 gggtgcggca gcggcatcat atccagggcc ctcctcctcc gggcccggcg ggtcgtcgcc
     6121 accgacgtca accccgccgc cttgggctcc ctccggggaa cgggggtcga ggccgtccgg
     6181 gccgacctct tctccgggat ccggcagcgg ttcgacctca tcctcttcaa ccctccgtac
     6241 ctcccgacgg gggaggagga ggttctggag gggtggatca acttcgcctt cgacggcggt
     6301 gagacgggga gggagacgat atgccggttc ctggaggggc tgaaagacca cctggagccg
     6361 gcgggggggc gcgccctcct cctcctatcg agcctctgcg gccccgggga ggtggaggcg
     6421 aaggagcgag ccgaggggct cgccgtcgag gtcgtccttc gcgagaggta cttcttcgag
     6481 gagctcctgg tcatgaggct caaccccgcg gaatcgttcg gagaatagga aacgtttaat
     6541 ccagatgggc cgtcatcttc atatccggcc ataagtaagg ggctgggacc tggcggcgga
     6601 ggggggggcg cgattttgct tttttcgagc ataaatgttg gaattgaata aacttaaatg
     6661 cgatgaacaa caacctgtta tagcagaggt gatcccgtga tgaagatccg agtcgtgagt
     6721 tcgaagaagg agatcgacga gctgaaccga aacgagcaga ttatacacct ggccttcaga
     6781 gcctccaaca ccgatatatt caggctcctc caggcctgtc ccagactgaa ggcgattcag
     6841 gttccctcct cgtattacaa gaccatgtcc cacgccggcc agatgtttct ggagatgcag
     6901 ggggtcgaga tcgtcgaggg ggacgtctgg ggccaccgga nnccggaagg acatcgacga
     6961 gtactacacc gtggacgaca aggtgatgga gaggataacc tccctccagt ccgaaggggt
     7021 caacctcgaa gagatcgccg gccgagtctc ccgggagtcg aagctctccc ccgcgatggt
     7081 caggtacatg ataaagcagg cggcttgagg gagcgggtgg ttcagaacct ccctcaggga
     7141 ttttggtggt atcatgatta agatcaggat agtgagctcc aaggatgaga taaa
//
LOCUS       DASM01000137           13409 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_13124, whole genome shotgun sequence.
ACCESSION   DASM01000137 DASM01000000
VERSION     DASM01000137.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13409)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13409)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13409
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_13124"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2753 a         3966 c         4344 g         2346 t
ORIGIN      
        1 tcaagagggt gacctacacc gaggccctaa agatagcaaa tgaaggccgg gagaacgaga
       61 tagagtgggg cgacgacctg gacaccgagg cggagagggc gataggctcc gccatcggcg
      121 agcactactt catgacggag tggccgacga cgataaagcc ctactacacc cagccctatg
      181 aggatagggc cgagatcagc cggggcttcg acctgatgca ccccacgatg gagcttgcaa
      241 gcggcgccca acgggtccac gaccccgccc tcctcgtcga gaagatcagg cggcaggggc
      301 tcgaccccga ggggttcgag ttctacctga gggccttccg gtttgggatg cccccccacg
      361 ccggctgggg cctcggcctc gggagggtcg tcctcacgat gctcggcctg gagaacatcc
      421 gggacgcggt cctcttcccc cgggaccgga gaaggctcgt tccctgagcc tcgggcagat
      481 ctggtggatg aagatccacc agccaatttt tttgccaacg ttgggccaat tccagaggat
      541 ctgatccaat gggagatggc ctcggatcgg cgtcttcgac cgagggagat gagtccaaaa
      601 actatatcta tcaacttatc caattgtcat tccatgttct tcagccccca ctccgaagga
      661 gggatggaat gaatttgttt gagaagatgc tgctcatatc gacccttctc tgcctcgcaa
      721 ccgcaggatc tgccgaggat gatgatccca tcggctggat cggagggccc ggaaccgaag
      781 agggggtcgg gatggcgaac cccgccgcca cctactgcac caatcaggga taccggtact
      841 ccgacggaag gtgcgtcttc cccgacggct cctcctgcga cgcctgggac tattatcggg
      901 gagactgcag ctacagcccc gagccggtga agccgatagg gatgccaaac cccgcctcgg
      961 cctactgcaa ggccctgggg tacacctccg tcatcaggac cgacccaagg acggggggag
     1021 aggtagggta ctgcctcttc cccgacggct cctcctgcga ggagtgggcc ttctaccggg
     1081 gctcttgcca gtacagccca acgccaaaac caaaacccca accgacgccg gtcccccccg
     1141 tccccaagcc ccaggccccg acggcgatct acacctgcac cccgccgccg acgggagggg
     1201 aggagacgat cctggacttt gagccgaagt cgtcgcctcc ggcggcggtc tacagccagg
     1261 ggagcttcgt cccctggagg agcttcgagg ggagcttccc cgccacagac cccaccctct
     1321 gggtcttcac ctcttcagga tggtcctgga acgggatatc gcccctggac gcctggatca
     1381 cggaggtcct ataccttccc aggaacgggg cggtgaacct ctacctgatg gacttcagcg
     1441 gagacgtccg gaggctggac ctggggtact ccaccacggg gtaccatcag atcgtcctca
     1501 aggccagcac tccggggctg atgatggcga tcttcaccct ggacggggtc ccgagcaacg
     1561 tcatcttcgt cagcgtcaag gccgactgga ccccctgcgg cctccgccca gagggcgtct
     1621 cgacgacggg ggtggacttg atcccctgaa ggccgatcca tatcgggatc ggccttcgcc
     1681 ggatctgatc gatgagaagg tgatgaattg aagacgacga gcatcttgct gctgatggcg
     1741 gccctggcag cggccagccc tgctgcggcc agcctcgtga cgatcgaggc cggcgattac
     1801 tcgatagcga tagactccga cctggtgaga aacgacaccc aggaggctga ggcgggaagg
     1861 atcacctacc ccctggtggt gagggggtcg gcgtgggcgg aggcggtggc ctcggcgatg
     1921 aacttcaccc gtcagacctt caccatcggg accgcctccg aggacctggc ggacgtgccc
     1981 cacgacctta tgaccctgga gtcgaacgtc gggatccaga tcgagaccta caacgagccc
     2041 ccggaggggg ggcttcccac cacccccttc ggcctcggcg gggaggtggc gaacctgacg
     2101 atctccgccg tcaaggacgt caccgtgggg aggatcaacc ccaaggagac gtctctatat
     2161 aaagcggtct gggagtacgg cgatggccta gcccttccgg ccgtctactt cgtcttcttc
     2221 gccgtcgacg accggaccct ctgccggatc gagtcggtct acggcgtctc cggcgagatg
     2281 gggccggagg agttcgagga gttcctgggg agggtggacg tcatccctca ggcccccccc
     2341 tcctcggacg aaaggtagag ggcggccttc tgaggtggcg gagggccctg cgggcggaga
     2401 cagcgcccca tctcgaaggg cgggcccttc cgcctcattt ttcgtcgaag atgctccatc
     2461 catctttgcg ggcggcaaga attccagcgg tcgacggctg gccaaaagat atatagcctg
     2521 gatgggttta aaataccgca taagtttggg ccgccatcga ggcctacctc cgacggcttg
     2581 cgagcgcttc agccagccag agaggaaagg gggagtggga aacgtccgaa aagatggaga
     2641 agaagaggga gaggatggtg gaggtctgcc cggtctgcgg gagctccgag atgtaccttg
     2701 agaccggcgg ctacgtcggg aaggtctacc actgcaaaga ctgcaactac atgggggccc
     2761 tggtggtgga ggcggacgag gagatggtgg aggcgataaa ggaggggtac ggccgggaga
     2821 agaaggggga agataaagga gatttaaaaa atagtttata aaaatcccaa actttgacat
     2881 attatgccga atataaacac caaagcccac gttctagtcc gtgacggctt tacatgtcgc
     2941 tattgtaacg ctagattgta tcttgctcag gcagtcaaaa tgctagccgt ccacaggccg
     3001 gggagcatcg actgggaccc ccactggcga agtgagcccc tgaagagcag cggggcgacc
     3061 gtcgaccaca tcatcccaga agacgagggg ggatacgata cttttgataa cctggtcgcc
     3121 tcctgcgttg tctgcaactc gagaaagggg aagggagaac gaagtatatc tccacagtct
     3181 tccgagagat cgtgggacgg tggaagcagc atatttctcg cccttgctcc aacttacgag
     3241 ggcagactga gcaaagagga cgaaaagtgg aagagggctt tggaacgaga gggtatgagg
     3301 ccggatattg aggcgataca ggagagaata aatattatat tatttttaaa cccctccaac
     3361 gatgacgcca gcgtctagcc agttcccgga tggactgaga cgttctatcc caaagtcgcc
     3421 agttttatga tgatgaaaaa agatagcctt tggccatgag gctcacctcc attctggtcg
     3481 tcttaacggt gatggcgacc ggccatcttt ctccggccag cgggatctgc gaccaagaaa
     3541 acctgactat cggtgcactg ctacccatga gcggcgattg ggccagcacg ggaatatcgg
     3601 ctcagatcgc cctggagcat gccgaggacg atatcaacaa tttcctttca gatatgaata
     3661 aatcgacacg gatacaaatc gtcgtaaagg ataccaggac cgacccagag gccacattcg
     3721 aggccttgaa ggagctggag gcccaagggt tgaggatcat cataggcccc gaggatagcg
     3781 cgtccctctc gagggtgaga gagtacgcca acgaaagtgg cataattctc ataagcggtg
     3841 gcagcaccgc cccgagcttg gccatctctg gcgatacgac cttccggttg gcctccgatg
     3901 ataccaacct ggcaaatgcg atggccgatc tgatgacgag gaacggagca aaagccgtgg
     3961 tggcacttgc cagaaatgac gtctggggca acgacctcat aaacgctacg gctgaaagcc
     4021 tgagccagat gggtggggtg aagatgaccc ccctgagata caaccccaat tcagatgact
     4081 tctctcagat cctaggagat ctgagggctc ttctggaaga cgcccgcgat gaatatggcg
     4141 atggcgccgt tgcagtctat cttattgcct tcgatgagtt gatagacatt ctgggccagg
     4201 catcgaggga tccggcgttg gcatcggcca gatggtatgg caccgatccg tcaggggtct
     4261 tgatcaggga ggatgagagg gcggcccagt tcgccataaa agttggcttc atatatccga
     4321 cattcagcca gggaaattcg tccgttgtca ggaggatcgc cgaggatatc agagaggggc
     4381 agaagatcga tatggtctcc tatgccatga acgactacga tgccgcctgg ctgatcactt
     4441 atacatatct tttgaccgga tccagcgacc ctgccgactt catgagggct ctccccgtct
     4501 ttgccaggac ctatagagga tattcaggcg atgccctcct gaacgatgct ggagataggg
     4561 ctttcgcttt ctacaccttc tggtccttga gagactcgga tggcataccc cagaatgtac
     4621 ctctgagcag atacatattc aatccctacg agggggtatt ttggatccct ggggatgagc
     4681 cctcctgatc cggagagctc ccccaggtca aaggaagaga tccaacgggc tgcaaaggga
     4741 tgtatttgga ggtggagata aaggggcgag ccatgccgga gcgctcactc cccccgcctc
     4801 accttccccc tcgccaggga cgcgaagatc ccgatgacgc agaggacgaa gaagaccgat
     4861 aggacggcgg tcaccgcctc cagaaactgc ggatagacct ccggggtcac ctggacccgc
     4921 cccatgaata tagagagggc ggtcatggcg atggccatgg aggccatcat cccgatcgac
     4981 ctcatcgtcc ccaccatccc cgaggcgacg ccgtagtccc tcttctctac agagctcatg
     5041 atggcgttgg tgttcggcga cgagaagaag gcgaagccga ggcccaggag agcgaggacg
     5101 gcgacgatga agcccaatcc cgtctcgccg tcgaggccga gggcaaggag gaggagcccc
     5161 gcggcgacga accccatccc ggcggaggcg acgacctggg cctcgatccg gtcggagagc
     5221 ctccccgccc agggcgatag gaccgcctgg acggcgggct gggaggcgag gacgaacccg
     5281 gccgcgaggg gcgatagccc cttcaccgtc tggaggtaga gggtcaggac gaaggcgacg
     5341 gcgaaggtcg ccccgtagtt tatcagggcg gcgaggttcg atagggcgaa gacccggtta
     5401 tcccggaaga gccggacgtt gaggacgggg cttctgctcc tcagctccac caggccgaag
     5461 gcgaggaggc cgaggaggcc gagggcgagg aagcctgccc ctgcggccga ggggaggctg
     5521 gtgaggccgt agaccgtcga gatgagggcg agggcgtata tcgaggagcc gagccaatcg
     5581 aacctctccc cggccgcctc cacccactcc ccctcgagcc tccggagagt gaggaggacg
     5641 acccacgcgc ccagagggac gttcaccagg aatatgctcc gccaccccag gtatgatgtg
     5701 aggatccccc ccaggaaggg gccaagggag aggccgaggt agacggcggt gacgttgatc
     5761 cccagggccc tccccctctc ccccggcggg aatacgctgg tgaggagggc gacggccgtc
     5821 ccgaagatca tggcgctcgc catcccctgg aagagccgga agaatatgag ggcggagccg
     5881 gagctgggaa aagcgcagaa gagggaggat gcggcgaaga tccccatccc cagggtgaag
     5941 aacctcttcc tcccccagat gtcggcggcc ctccccaggg ggaccatgaa gaccgccatc
     6001 gccaggaggt gggaggtgac cacccacccc aggaggacgg cgtccatccc gaactcgacg
     6061 cctatcggag gaagggcgac gttgatcgcc gagttcgcga aggccgggag gaaggcggcg
     6121 acggtggcga tgaagaggac gctacttttg ctctcggctt cgcacatccc ttggggattt
     6181 ggagaggaga tatatacctc ctcccctcgg tcggagctgg gccgggcgga ggggatggac
     6241 aaatataaat cgcatacgaa attaatatat cgaccatgaa gatcgtggga atatgcggca
     6301 gcccccggga gaaggcgacg gaccacatcc tcaaagaggc cctcaggatg ctggaggaga
     6361 ggggctttgc caccacctcc ttcaccgtcc ggggaaagag gatcagcccc tgcctccact
     6421 gcgacttctg cctcaccaac aaggagtgca ggataagaga cgacatgtac cagctctacc
     6481 cccttctcca ggaggccgac gggatcgtct ttgcgacccc ggtctacaac ggcggggtca
     6541 gcgcccagac gaaggcggtg atggaccggt gccgggccct cgtggccgcc gacttcgact
     6601 tcttcagggg gaaggcaggg atggccgtcg ccgtcggcgg cgatcggatg gggggccagg
     6661 agctggccat cactcagatc atcgccttct acgtcttgaa cggcgtcata cccgtcggcg
     6721 gcggctcctt cggggcgaac ctcggcgcca ccttctggtc gaaggacacc ctggagggtg
     6781 cgatggagga cgccgagggg tggcggtccc tccggaagac aatccggagg ttctccgacc
     6841 tcttgggcaa ggaaaaataa atcaaggggt ggatggataa ggatagaaga tgactgagaa
     6901 gaggcccaag aagaagaggg tcgtctgggg gatcacgggg tcgggcgacc ggatccggga
     6961 gacggccgcc gtgatgaagg agatgaggcg aaagtacgac gatgaggtcg agatccgggt
     7021 ctacatctcc aaggccggag accaggtggt gaagtactac aagctcttcc cggagctgga
     7081 ggaggagttc gacaagatct gggtggaggc gaacgccaac gcccccttcc tggcggggca
     7141 ggtccagatg gggaggttcg agttcatcct catcgcgccg gccacctcca acaccgtcgc
     7201 caagatctcc ttgaggatcg cggacaccat gctcaccaac gccgccatca tgagccagaa
     7261 ggtgatgctc ccgctgtaca tcatgccctc cgacttccag gagggggagg tggtgacgac
     7321 cctccccgac gggagggacc tgaccctcgt catccgaaag gaggacgtgg agcacgtgaa
     7381 gaggctggcg gcgatggagg gggtgacgat gctggagagg cccgaggata tcccgaagat
     7441 cttcgagagg cacttcggtt gaccggccgc tccgaaggag ggttgggcgg aggaaaaatg
     7501 gatcgccgag ggacggacgg atgaggatag atggatggca gagggacgga tcgaggctcc
     7561 tctctgaagg tggcgaaccc cccatctccg ccaccgacca ccagaatctc agaattgaat
     7621 aggcattccg ccgatcagga ggacttcagc tccttcagcc tgctgatctt ggtcatcacc
     7681 tggtcggtct cggtcttctt gttcgtctcc aggtaatcga tgatgtcgta gagccccttc
     7741 ttcagcttgt atatccgata gggcctcccc ttccccgggt tcttctcgtc cctctcggag
     7801 atccagtcga gcttccggag ctccctcatg gctatgctga cctcgggctg ccggaggtcc
     7861 gagcccatct ctatctcccg gctggtggct tcgtcgacgc aggcgaggta ggtcaagacc
     7921 ttagccacat tccttttgag gcctatcttc atcaggatcg atgcgaactc ttcgcgttca
     7981 acggtctgag tgggtagcga aacattcctc aatccggtca ccattgtact ggtagttata
     8041 gatccatata taagtttcgt tcaaaataat tatcatctaa tccggaccgg gcgcgccgtt
     8101 cctctggacg ggacccccgg gatcgaaagg gattttaccc gccccggccg agatacaatc
     8161 cgagatatta tctggattga gaggcgatct cgtgatcagg ctatattcca ctgacagcta
     8221 tcttaagagc ttcgagggga gggtggtgga ggtcttggat gatgaggtca tcctggataa
     8281 gaccgccttc taccccgact ccggaggcca gccccacgac accggaaccc tggccatggg
     8341 cggcgagtcg tttccagtcg tctacgtccg acccgccgaa gacaatatcg cccaccgggt
     8401 ggggaggccg ggcctggtga ccggagacct ggtgaggggg gagatcgact gggagaggag
     8461 gcacctcatc atgcggagcc acaccgcctg ccacctcctc tccgccgtca tcttccagag
     8521 gacgggggcg atgatcacag gaaaccagat cggcgtcgac agatccaggg tcgacttcag
     8581 cctccaggat ttcgatcggt cggccctcgc cgattacgtc gagacggtca acgagatcat
     8641 ccggcaggac cgggccgtct cgatccgccc catccctagg gaggaggcga tggagatccc
     8701 ctccctggtg aggctggtca aagaggtccc cgaccgggag gtggtgaggg gcgtggaggt
     8761 ggaggggatc gaccttcagg cctgcggcgg aacccacgtc cagaggaccg gcgagatcgg
     8821 caggataaag atgctgaagg cggagaacaa ggggaagacg aaccgccggg tctacttcga
     8881 agtccagagg ggggattgag cttggacgag atggagcttt tgggaaggac gcgggtcgtg
     8941 gtcaaggaag gccgggtcgt cgagaccggc gagcccctcc tccggtggtg ccccctcttc
     9001 gataaggtcc ggggtataaa ggagatcacc cccgaggcgg cggccgccaa catggagttc
     9061 cggatgagag agcacgggat gttcaccccg gctcgaaggc tggagatgga cgtctttgtc
     9121 ggcttcggcg cctcagaggt gatgatgacg gcgacgcggc gggggctgat cgaggcggca
     9181 gtcaccgtct gcgacggcgc cgggacggtg gtcaccgcaa accccagcct catccagggg
     9241 atgggggggt ggatatcggg gctcgtcacc accgatccga tccccgaggt cctagccggg
     9301 atcgaggccc ggggcggggt cgtcttggac ccggcgaccg ccaggataga ccaggtggcg
     9361 ggggcaaaga tcgcggccga gcgatacaaa aggtttgcgg tcaccgtcgc cgatgccgag
     9421 accgccgaga ggctccggga cctggagaag gagagggacg tccggatcct gatcgtgggg
     9481 gtccacctga cggggatcgg ggaggagggg gcggagaggc tcctcgccgc cgccgacatc
     9541 gtcactagct gcgcctccaa gcccctccgg gagaaggtcc ggcccctcgt ccaggtgggg
     9601 acggcggtgc ccctcttcgg cctgacggct tggggtaagg aggtcctggt ggagcgggcg
     9661 aaggaggtgg atcggcctat cctgataaat acgatgcctt tgccggtcct cccggagaat
     9721 aagcagcccc gccccctggt ctaggggggg gccggagaag ggagttggtc tggccccggc
     9781 tcaggggcat ggtaggtaga gctcgctccc cgatccgatc tcctgccgga tgttgaactc
     9841 tccgatgtag gtcccgccgt aggtcatgat ggcggtgccc cggcaggggc catcctgcac
     9901 ccgggccccg aggcgagcgg tccccctccc ctgggaggcg acctggtaga tggaggcgtt
     9961 gtcgttctgg aagatggtgg tgtcctttat caggtagtcc aggtcggtgt agctctcgga
    10021 tatgaccgtt ccaagcctgt agttcttggc acagagggcg tttcgaagat cgctctcgcc
    10081 ggtcccgtcc tggacggacc ggtggtcgaa ggtcccccac tcgttgaagt acatctcgtc
    10141 gtcgtagacg taggcggtct gcctcctgtc gatcatgccg gatcccctcg tcccgacgac
    10201 gagcttctga ccgtggaaac ccctatcggt ctggtagacc ttcatctcgt cgatgtagcc
    10261 gctcaggtgg acgtaagaat gctctgtgac gtccttggag atgcgccagt cagagctcca
    10321 tgcagcaaag gttgcaggca tcgagaggat catggcgagg acgaccagcg gcagcaaaac
    10381 tctgaccttc atcatatacc cctttcctat tcccatattc tggcacatcc attataaatc
    10441 tgatgggtcc cgaacccccc cgagatcccg ataggctcca gcgatcgaag atgccacgat
    10501 tccgcctcca tcaccaggaa cccaggcgga ggacctccag ggccggcctt tgctgggggg
    10561 gggcctcttc cgccaggagg taatcgaccc cggggctcct ctcggcgatc tccgttccga
    10621 cgacccggtt tttggcccgg agggcagatc tgaggtggct ctcagcctcg cggccgtagg
    10681 tggtggtctc tgggacgaag gtgtaaggat ggtagttgta ctcggcttgg atgttgaggt
    10741 atatcccgct cccgccccca tcaccgccgc agttgccacc acagctcccg ccctcgtcga
    10801 cgtagacggt catcgtcttc gaggcggttc cgctaccgct gacgctgtag gcgagcttgc
    10861 ttccagccgc acccctctcc gtctggtata ctcccatctc gttcatatag ccgatcagga
    10921 atatcgacga ctggtgggtg acgtttttgg cgatcttcca gtccgcatcc ttgcatttct
    10981 gatcatcgca gcctccaaga gccgtcggag gggcgagcag aaatagcacc acgccgagga
    11041 gcaccgccag tcctgccttc atcatcgacc tctcttccct ctggggagat atgaatgccc
    11101 gtccgttata aatctgatgg gctctcgccc ctaacccctc ctccccctgg agggcatccc
    11161 cggcctcctc tttcccgcag ggctcgccct gatctcatcg atccggcggt cgagggcagg
    11221 cttcagccga tcgttcacct cgccggagaa gcggtcgatc ctggcagcga tggcgagctt
    11281 cgccgcgaga gcccgagccg ccctcccccg gagcctccgg ggggtcccca tgaccgccgg
    11341 atgccggtag atcatgccat gcttcggcga aggcgccttc ccccggaggt gcttgaagag
    11401 ggacttctcg gcgcccatca cctggatcgc cgaggcgggc atctccgaga ggcggccgag
    11461 ccccccggcc cgggagatga tcctcgcggc caaaagcggc cccgcgaggc tggataggtt
    11521 cggggcggcc tggaccgtcg ccgtctccag gctcctctgg accgaccggc gggcgtcggc
    11581 gagggcgagg acggaccggg cgacgacccc tatcgcctcc tcctcctcgg cgagggcctc
    11641 cgccaggcct cttccccgga ggcggcggtc gtcgtggagg cgggaccact cgtagagccg
    11701 ctcctcaagg cggtttatca cctcgttcag gtcgtcaagg gcctcgatcg tctggaggag
    11761 ctccgcctcg gccccagtcg aggcccggag cctcctcctc accagctcca tcgccacctc
    11821 tcggagggcg gcgttgtact cggcgtcgtc ggggaagagc cccgcctcca gggcggcctt
    11881 ccgtaggtcg ggctgaaccg ccggcccccc gacggagccc tcccaccccg aggaggcctc
    11941 ctcccccaaa acccccatca gcccttcgac gatctccgcc gcccctccgg ggaggcagta
    12001 agccccgaat tcgtcgtcga tatgcccaaa ccaagtcctc atcttcatct caatatccct
    12061 tatcccggcg gggccaatat tactttcgcc ccgccagaag caactttatc taccttgaga
    12121 gcctctgggg tattgcctct ctattaaaaa ctggaggtca aaagattatg aatgcagccg
    12181 tagagcagat tctggctcgg ctgcggaacc agaaggtgga ggttcctgca tccgaggtgg
    12241 agagccgtct ggccctcctg gtggagaagt tcggggtccc tgagggagag gcggtccgat
    12301 ccgtcgtcaa ctattttctc aaagagcacg gcgtctcgcc gacgaccttc gccccgaaga
    12361 aggccgagat cctcaaggtc ggcgagatcg acggtccagg ccggtggata gacctggagg
    12421 cgaaggtggt ggagctctgg gagccgacga gcagcgccat atcccagacc ggcctcctcg
    12481 gagacgagac ggggacgatc aagttcgtga agtggaccaa ggccgacctt ccggagctgg
    12541 aggcggggaa gagctacctc ttcaagaacg ccgtcaccga cgagtttcag gggaggttca
    12601 gcgtcaagct gaacaggacg agcggggtcg aggagctgac gaaggtggtg gccgccaccg
    12661 agccttcggc gtcgagccag gtccaggaga tgaaggtatc cgagatcaaa gagcaggggc
    12721 ggtggataga cctcagggcg aaggtggtcc agctctggga gccgacgagc gagacgatat
    12781 cccagaccgg cctcatcggc gacgagaccg gcaccataaa gttcgtcaag tgggcgaagt
    12841 cggggatccc ggacctcgcc gaggggaaga gctacctctt caagaacctc gtcaccgacg
    12901 agtttcaggg gaggttcagc gtcaagctga acaggacgag cggggtcgag cccctggagg
    12961 cggaggtgga ggcctccgat gcccccggcg gccaggtcca ggagatgaag atctctcaga
    13021 tcggagagga ggggcggtgg atagacctca gggcgaaggt ggtccagctc tgggacccga
    13081 cgagcgagac gatatcccag accggcctca tcggcgacga gacgggggtg atcaagttcg
    13141 tcaagtgggc gaagtcggag atcccggacc tcgccgaggg gaatagctac ctcttcaaga
    13201 acgtcgtcac cgacgagttc gaggggaggt tcagcgtcaa gctgaacagg acgagcgaga
    13261 ttctgcccct cgaagaggag gtcgaggtga gcccccagga ggcgaatttc accggagcga
    13321 tcgtcgacgt ccagaagggg tcggggctga tcaagcgttg tcccgtatgc cggaggatgc
    13381 tgaccaaggg ggtctgcggc gagcacgga
//
LOCUS       DASM01000138            8889 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_188390, whole genome shotgun sequence.
ACCESSION   DASM01000138 DASM01000000
VERSION     DASM01000138.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_188390"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1439 a         2974 c         2826 g         1648 t
ORIGIN      
        1 acggcccgcc ggggacggga aagactctca tcgctagggc cgtcgcccgg gagacgaagg
       61 cgaacttcat atcggtgaag ggcccccagc tcttatcgaa gtgggtcggc gagtcggaga
      121 aggcggtgag ggaggtcttc aagaaggccc ggcaggtctc ccctgccatc atcttcttcg
      181 acgagctcga cgccatcgcc cccatgaggg ggatggagga ggggcccagg acgagcgagc
      241 gggtggtgaa ccagctcctg gcggagctcg acggcctgga gaccctcaag gacgtggtgg
      301 tgataggcgc caccaacagg ccggacatca tcgaccccgc cctcttgcgg tccgggaggt
      361 tcgaccgcct cctcttcgtc ggcccccctg atcgggccgg gaggctcgag atcctccgga
      421 tccatacaaa aaagacgccg aacggggacg acgtcagcct ggaggagctg gcggagctga
      481 ctgagagctt cgtcggctcc gacctcgagt ccctctgccg ggaggcggtg atgctcgccc
      541 tccgggagga cccggaggct tctgaggtgg agatgagaca ctaccgggag gcgctgaaga
      601 gggtcaggcc cagcttcgag gagaatatgg ggcggtacta cgagcggatc aacgagcggt
      661 tcaggggcgg cgtcaaggtc gaccccagct cctatctggg gtaccggtga ggcggattat
      721 gccgaagatt tacgcccgga gcctcgccgg aaaggagatt gtgaccatgg gcggcacggt
      781 cctgggagag ctggagaaca tcatcctcga ccccgagacg ggacggctga tcgacctcct
      841 cgtcaagccc gacccggaga tcgaccccgc gcggtaccgg cgggagggga agctcatcct
      901 cgtccccttc tcctcggtct gcgccctcaa agactacatc gtcgtcgacg agaagagggc
      961 ggagaggtag agggggcggg gcggccggaa gaacggtccc gcggagggat ttgatcttga
     1021 taaagcccat caccggcctc tttctcgtct tctggggggc ggtgaaggtc tatcaggggt
     1081 ggctgatcct cgatggaggg gtggagttcg gggctgccgt catcctcctg ggaggcctgg
     1141 cggcagcagt cggcggcctc tttctcgccg actcctggag gtgagacgcc gactttcctt
     1201 tggagctcgt ataggtatga cgggcctcct ccatcccgcc tcaggagcct ctgataccat
     1261 caacctctct ctcagccggg aaaagtataa cttaccccgc ctccaccatc cctcgacgac
     1321 catgaccaga tcttccatcc ttctgggaat cctattcatg ctcgcaatct ttggagctgg
     1381 ttgtttgaca gagagagagg agaaggcttt ggggccggag gggacgatcg tcctcctgga
     1441 gacgaacatg ggcgagatca agatccggct tcgcgacgac atgcccgtca ccgccggcaa
     1501 cttcgagaag ctggtggccg acgggttcta cgacggcgtg atcttccacc gggtcatcga
     1561 cgggttcatg atccanngga ccatatcgat ggccaatgca ggcccgaaca ccggtggaag
     1621 ccagttcttc ataaacctcg tcgacaacaa ctacctcgac aagatgcacc ccgtcttcgg
     1681 cgaggtggtc ggtggcatgg acgtggtgga caagatcggg agggtgaaga ccggcgccgg
     1741 agatcggccc acgaaggacg tggtgatcgt cagggccagg atcttatcct gacccctccc
     1801 ttttggtggg ggctgctgtc ctcgatccgc tctgtcacaa cctgtaaata ccctccctcc
     1861 cgatggcata gaaagaccta tgggggagac tgaggctgga tcgaggagag ttgagggacg
     1921 gggcgggttt attcgccatc tttcttctcg gcctccttct gaggatggcc ccgatgaggg
     1981 gcgccctcgt cggcggagac atcctcttct acggctccga ctccttctac cacatgagga
     2041 ggatcctcta caccgtcgac cacttcccgt cgaccctctg gtttgatccc tccatcaact
     2101 acccggaggg gctggagctt ctttggcccc ccctcttcga ccaggccgtg gcgggggccg
     2161 ccctcctcct gggggtggga tctccgaggt cggtggagat ggtagccgcc gtcgcccccc
     2221 cgatcctggg gtctctgacg atcatctccg tctacctcct cgcccgggag ctcttcggcc
     2281 cccgggccgc cctcctctcg gccctcctct tcgccatcaa cccccagcac gtcagcatca
     2341 gcatcttcgc ccggccggat caccacgtcc tcgagacctt catcctggtg agcttcgtcc
     2401 tcctcctcgt cctggccctg aagagggggg atggatggcc ctggcctggg gccgccgccg
     2461 ggatctcggc tgccgccctc gcctacacct ggatcggagc cgccgcctac ttcggggcgt
     2521 tcctggcctt cgtcatcatc gccgccaccc tcgacctccg ccggggggtc tcatctcgcc
     2581 cctccctcca ggtattcctc ctcgtcttcg gggtcgccct cgtcctcgcc ctccccttct
     2641 ggtgggtggg gtggatggcc ccctcatttt tcgccctcgc cgccgtccta gtgacgacgg
     2701 cggccctcct ccccctcacg ggggcgttct ccaggagggg cgtcccctgg atcgctctac
     2761 cccccctcct cctcctcctc ggctatctcc tcttatcatc agtcttcgcc ctcgaggggt
     2821 ggctgggggt ccaacggctc ctcgacgagg ggatcgacta cttcttcggc ggcgccctct
     2881 cctggcaggt ggcggaggcg gtccccctct acggcgtggt caggcccttc accgtcgttg
     2941 gattcaatct gacggcggcg gtcctgggat tcgcccacct ctggcgttcc gacatcgata
     3001 gactccagct cctcttcctc gtctggaccg tctccctcct cctcctcgcc gtcttccaga
     3061 accggttcct ctacgtcttc tccgccagca tggcggtcct catggccctc ttcttcctca
     3121 gggccgccac cctactctat gagggaggat ggcggctagg agacccgagg aaatcgaggt
     3181 acctgaaccc cctcctcctc cttctcctcc tcctcccctc ggcggtgagc gtctcggagg
     3241 tcttctcgat ccggccggcg gtggtggagt cggggtggga ccgccccctc cagtggctgg
     3301 aggagaggtc ggcgcactcc tccgccccct gggaggggga tacggccgcg ggggccgggg
     3361 tcctcacctg gtgggatcgg gggaactggg tcctgtacct ctccggttgg ccggtggtgg
     3421 caaacggctt ccaggccggg gccgaggatg cggcccggtt cttcatcgca gaggatgagg
     3481 cggcggccct ggagatcatg gacgcgagaa gggcccgtta cgccgtcacc gagtcaaggc
     3541 tcctggggcc ggggcttgcg acgatcgtcc tctgggcggg ggaggacccc tccgagtacg
     3601 gaaggatggc cggcgccggc cctgaagggg gaggaaggtt cctgaggacg accctggccc
     3661 ggtgtcacct caccgactgc cgggagatga gctccctccg gctggtctac gagtcagatc
     3721 cgggggtggg gtctgatcag gtcaagatct tcgagcgggt ggctggggcc cgtatccggg
     3781 gcgcctcccc cggggatggg cccgtcaccc tctctctgaa cctcaccaca aaccagggcc
     3841 gacccctgaa ttacaccagg agcgctctcc ccaggggcgg caggtacgag atggtcgtcc
     3901 cctactcgac gggcggggat gggggcctgg cagcacgcat cgccacctcc ggcggcgaga
     3961 ggaggggcat cttgatcagc gaggatgctg tcctccaggg aggggagatc accgtcgacc
     4021 tctgacccat tcaccttcca aaagtcttaa agccaatcgg taagaatcga tcccgtcgga
     4081 taactcctat gtagaggagg cggcccctct ccggggatga ccggatcggt ggctatttta
     4141 aagatgccgc tacatgggat ctctgcgagg atatgctatg aaaccggctc taacgttcat
     4201 gctcgcgatc gctctcgtgg tctgggcccc tgccgttcag gctcaagagg tcgtggatat
     4261 ctactcggac cttcagtcct gcgacgtcac cgttacgggg gatgtggccg ggtgcgtcct
     4321 ccagctggat ctatcatcga ggaaggcagg cccgatccag acgaaggccc tcgatctcga
     4381 cggtcctggg acctgggtcg ccacctggga cgtcggcggg gctgaggatg gcagctacac
     4441 cgcctgcgcc aaactgatcc gggagggcga ggcgatctcc gagaagtgca gcgacttcca
     4501 ctacggcggg agggtgagca tcaggttcga cgtccgggac tcccacgccg accaccgggg
     4561 tatgaacctc ctggtctatt ctggagaccc ggcggtggtg gacatctact acatgctcct
     4621 cgagggcgat aaggcggttt acgtatcgaa gagggaatcg gtccccatct ccggctctta
     4681 cagctccccc tccgacctct ctctggagtg gaagcagctc ctggagaacg gccgcgagta
     4741 cggggggcgg gttaagatcg tcgagaagaa gaacggtcag accagggcct tcatgaaccg
     4801 gttcaccgcc atagacgacg ccgagatcac cgacacctac caggacgaga ccggggccag
     4861 cgccacggtg atggggagtt cccgggtccc cttcgagggg tccctccgct tcgacctccg
     4921 ccagaacggc gcccccctcg tcaccgtcga ggagaggacg ccgatcctct tagccgggga
     4981 cgacgagacg gtggagatat cctggaacgg aaccctggag ccggggatct acaggctcac
     5041 catccagctc ctcggaaacg acggcgaggt catcgatatg gaggagagca tcatcgaggc
     5101 gaagatcccg ccgaggcccc cggccgtcgc gccgccgcag gaggaggggg gcgggggcgc
     5161 cctcaccatc gccctcgtcg tcacgatcct ggtggctgcc gccgcggctc tcttcttccg
     5221 gcgcaggaga ggtggatagc ccgagaagaa tccaagaggt cagagggcgg gaccgacgga
     5281 gggccccagg tccggtccgg ggcccgacct ctcaccggcc ccaggccgcc tcggcttttt
     5341 atcagcgatc ctcagccgct cttctcggtg tcggcgacga cccccggacg acggggtttc
     5401 tgagggtccc aagcccctcc acctccacct ccaccaggtc tccggcggag agggagccga
     5461 caccgggggg cgtccctgtg gctatgacgt cgccggggag gagggtcatg atcccgctta
     5521 tgaactccac cagctccgga atcgagaata tcaggtccga ggtgttggag ctctgtctca
     5581 cccttccgtt gaccctcgtc tcgatggcga ggctcgaggg gtcggcctcg acgacgtagg
     5641 ggcccagggg cgcgaaggtg tcgaagctct tcgccctcgt ccactggccg tccctcctct
     5701 ggaggtcccg ggcggtgacg tcgttgaagt tcgagtagcc gaggatgtac tcctccgcct
     5761 tgccggcgcg gacgccccgg caccttctgc cgatcaccac cgccacctcc ccctcgtagt
     5821 cgacctggct gctcgaagga ggcatgacga tatcggcgcc ggggccgatc accgccgtcg
     5881 gaggcttcag gaatatgatc ggctccgtcg gaaggtccat cttcagctcc tttgcatgga
     5941 ggaggtagtt gaggccgacg cagacgacct tgctcggctc ggcgggggcg aggatcgcga
     6001 cctctccgat ctcgtaagcc ctgccgtccg cctcgatgga accgtcggac acttccccct
     6061 ggcggacgac gccgtcaaat aggaacctgg cgatcatttg attcatccat cccttaatat
     6121 ttttatgccg ctcgcccccc tcctgtaacg gccgaactcc gtcccggcga gcctgtcggc
     6181 cctgaagacc ggaccctcga cgcagaccct gatcccggag gggtctaggg agcaggagcc
     6241 gcagatcccc ataccgcact tgaagtagcg gtctatggac gcctggatct tcgtctccat
     6301 cccggcgcac ctgccgatga cgtcctccat catcggctcg gggccgcaga ggtagatctg
     6361 gtcgaactcc tggagccgga gggccgaaag ccccaccgag gcccggccgc atatccccag
     6421 ggagccgtcg tcggtcgtta tcaccacttc cccgatccgc tcaaacctct tgaggaagag
     6481 gaggtcgtcg caacaccggg aggcgaggag ggtggtgacc tcgatccccg cctccgtcgc
     6541 agcctccccc aggagggcga ggggggcggc ccccaccccg cccccgacga ggaggatcct
     6601 ctcgccagag aggttgaagc cgtttcccag ggggccccgg aggccgaggc tctcgccgac
     6661 ctcaagatcg aagatagccc tcgtcgcctc ccccaccagc tggacggtga tcccgtctcg
     6721 ataggagaag ctcatgggga cctcgtcgat gcccctcacc cagaccatca ggtactggcc
     6781 tgggaccggg ccgagggtgc ggtcgaaccg gatggtcctg accgttggag tctcctgtac
     6841 gatctcgacg atccgggcgg agatcggcct catgtcgccc tcctggcaag ccccaccagc
     6901 tcctccaggt cgatcccctt ccggtcgagg tatgcggaga gcccctcggc gatggacctg
     6961 aataccccgt acccttcaat cattcccagg gccgtcccca cctggacgcc cctggctccc
     7021 gccatcatca tctcggcggc atcctcccag gaggcgatcc caccgacccc gatcacgggg
     7081 atctccaggg cggaggcgag gtcgtagacg cacctcaccg ccactggctt tatcgcgggg
     7141 cccgagaggc ctccgaacct gtttcccagg atcggcatcc cggactcgac gtcgatggcc
     7201 atcgcccgga gggtatttat cgccacgacc ccgtcggcgc ccccccgctc ggcggcgagg
     7261 ccgaggctga cgacgtcggc gacgttgggg gtgatcttca cccagaccgg gacgtttacc
     7321 tcctccttca cggcccgggt gatcgcctcg acgttgcagg cctcgcatcc gatctcggcg
     7381 ccgagcctat cggcgtgggg gcagctgagg ttcagctcga agccgtcggc gtcgaggccc
     7441 gtcgccacct cgacgaactc ctcggcgccc gcgccgaaga tgctggccac caccggggcg
     7501 cctccacccc ggccgatatc tatctcctcc tgaaagttcc ggtaagacgg gtttggaagc
     7561 cccatggcgt tcaagagccc cccctccacc tggacgacgg tggggccccg gtgcccctcc
     7621 cggggggcga cccccaccga cttggtgacg acggctcccg ccccggccag ggccgctctc
     7681 ctgagggagg cgccggtagt ccccaggatc cccgctgcca gcatcacggg gctctccatc
     7741 cggattcctc ccacctctcc cgaaagggat gccatgacgg gagagtcgtc aggggaggat
     7801 aaaaggcttc ccgaagacgg ttcttctccg ggaagcgggg atacgtcggc agttggccat
     7861 ggggtgccgt ccccctcagg aggcgtggga ccagacggga cccgtggagc tcatcgaggc
     7921 cggcgaccga taccggcctt ggggggcgga tggtcgcttc cttccgtcca ggcctccgat
     7981 tccctcatcc ccgaggcggt ctcgacggcc gagatcgatc ctccttctgc ctccgatgag
     8041 ttgatcacct tcaccgggag ggatggatct atcagggtca cctctcccgt ggagttgacg
     8101 atggtgatcg tccccgtcgg gttgatcagg gttataggag ccgtctggtt caccagaacc
     8161 accggctcgg acccgccgga ggagctgaag gggccggtct ctttccctcc ctccagggag
     8221 tcgtagtagc tcaccgcccc atcggtctcg ttccccgtct cgttcggcgg ggcggcctcc
     8281 acctcgatgg cgggttttcc ggggctgacg ttgacgactg gcttcgtcgg gttgacgctg
     8341 accttcggct ttgccgggtt tatgttgacg ttgagccgag agatgttgac gtcgacctgg
     8401 ggcttggtgg gtttgatatc gatgttcagc cccttcggtt tcgtccccgc cgcctcatcg
     8461 gcgctgatgt tatcctcgga gacgttctcc tgcgccggcc accaatccgc tgggttgaac
     8521 tcgttatcgg tggagtcgtt cagctcggcg atcccgtagt ccaccagcat cctgttgaag
     8581 gatggggata ggagggggct tccgtcgagg ccggagaggt agaggaccgc taccagctcc
     8641 ccctcggagt tccgtccgtc ctcggagctg tcgtcaatgt ccagccagac ggtcttgttg
     8701 aggaggatcg ccaccgccag gtccttcgcc gatgggccca cagggaccga cccctccgcc
     8761 gtcacgttgt tggtctccaa aagtgatccg ttcgtcatct cggcagaagg ctccatctgg
     8821 ggttcttcct ccgcaccgat gagatggccg ctcctcgacc ccttcatcgg ctcgtcgata
     8881 gatccgttg
//
LOCUS       DASM01000139           24214 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_84413, whole genome shotgun sequence.
ACCESSION   DASM01000139 DASM01000000
VERSION     DASM01000139.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 24214)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 24214)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..24214
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_84413"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         4485 a         7738 c         7844 g         4141 t
ORIGIN      
        1 agggggtcgt gccctgcgcc gtgagggggc ggaggcacgt tgaatattat taaaacagat
       61 tctgaattcg ggatcacaac ccgaaccgcc ggtaggtcat cgccgaaaag gagctgacgc
      121 ccagcgcctt cgagaccagg gcggcctcct cccccctcac cggccggccc ccggcggggg
      181 ggatcggctt tcccttttgg gcgtagacag cgcccccggt catcttggcg cagatgaagc
      241 ctccggcgtc cccggcgacg aagatctcgc cccccctcat ctcggcggcg gtgaagtcgt
      301 cggcctttcc ggcgatgacg atccgcccgc ctttcatcaa aacgcccgcg ttcctcccag
      361 cgttgcccat tatttctaga gagcccgaca tcatcaggat cccggcgctc atcccggcgt
      421 ccccctccac cctaagcctc ccgccggatc tggacctcgc cgccaacgtc tcccggagga
      481 ggccgtcctc caggacgagc tcgcccccgt cgatgcggtt gggatccagg acgaggcctc
      541 cccgctccag aacctccgtt atcgagatgt acttccgata tcccgtcaga tccgactcga
      601 cctcgaggac gtttcccagg ggctcggcca cgcttccgct tacatagaac gagccccgga
      661 gcatgctgat ccccatccgg ggtccgacgt ccccgtcgac gacgatggtg ccggtctcct
      721 cgatgggacc gccggtcccg ccgaagctcg ccaggtctac ccccaggctc gagccgaggc
      781 ggccgccgac gtcgccccgg atcatcacgc cctcgccccg cctcagggcc tccaccagcc
      841 ccctccaggt ggtggcgccg gagacgaccc cttcggggtc gaggcgggat ccctggtgct
      901 gccagtagaa gtcgaaggtg aagtcgcaga ggcagctctc gcccccggcg tctatctctt
      961 tcataggagg ggccccggaa cgagaggcct catctgcccg ccaccaccct cttggagatc
     1021 ttcaccccga cgagggcgat gatgatggca aggatggcgg aggccgccag gtactgggtg
     1081 ctctgccccg agatgagggg gaaggcgacc ccggcgctgt tgacgaggat gaaggcgctg
     1141 gcggaccaga atatgaggcc ggtggcgaag atgaagaagg ggtacgagtg gtaggtgtag
     1201 gaccgctcgt ccctcccgcc ctcgaggtag aggtccagga tcttgccgag gttgacgcag
     1261 atggcggcgg cgacgtacca ccagacgctg tagttgacga agaccatgac caggactatg
     1321 tacccgtacc atatgtcccc ggtgtagtag tcccagaacc tggcagaccc ctggatcgtg
     1381 gcgatcagga ccaggaccgc cgccaggacg taggtgacga aggcgacctt cccgcggtat
     1441 aggttcccct tgaaggtctc cttggtgctc tcgatgaagt cgtcgagccc cagaccccgg
     1501 aagaggaggt agacccccac ggcggcgagg atggcgacga cggcgaagtc cgacctcccc
     1561 aggaagctga aggtcgagta gatgaggagg gtaaggccta tcggtatgaa gaaggtgtgg
     1621 ctgatcttcg nntagtaggt gctctccagg ttgtggctct gcttgacgag gaccctcttc
     1681 accccctcga ccttaacccg ggactggatg atcgggagga tcgcctcgtc ctccgccccg
     1741 tcggagacga ccaccacccc ctccggcccg aacctctcca ggaggtcgtc tatctgatgg
     1801 gctatcttcc cgtcggagac gaggccgacg ttggggtccc cagccaccga ggcgatctca
     1861 acggtccgcc cctgggaggc gaggtcgtcg tagacctgga tcccgccgag gatggtgttg
     1921 acgtccgagt cctcgggatc ggcgaggccg agggagaggg tcgcctcgac gttggcggcc
     1981 ctcccgacga cggggctatc gatccccgcc ttccggccca ggtcgtcgtc tctgtcgatg
     2041 cagagcacca ggatttccat accgtcctca aatagggata agaacgtact taaagattct
     2101 ccgagctcgt cctatggcgg agaaagtttc gatggaaaaa ggctaataat cggcggaaag
     2161 aaaccctgag gccatgaaga tggccttatt tctcctggtg gcgacgctgg cctgggccgg
     2221 atcggcaccg gcctccggcg tcgcctttga ggatattcag cacgccgtcg agatatacaa
     2281 catgggcgta gagagcgcac cagaggtggt aaagaccctt cttggagacg aaagggtcca
     2341 gatcgaggta tccggcgagg aggggccctc ccttctggcg ggcctcgaga cgaggagggc
     2401 cgtggtcgtc aacatcaccg agggggagat cccagacccc acgatcctgg tggaggcgac
     2461 ggaggatgcg atccaacggg tcttcctctc ccccgatccc gccggggcct tccaggaggc
     2521 gagagaccaa ggcgatataa aaatcaccgg caacacctgg tcggcgaagg cgaaggtggc
     2581 ggcggccctg gccagctcct cagccctcca gttcttcgcg gggctcatcg gaggatgagg
     2641 atcagatcct gaggtatacg tcttgatgtc tacctcctga ggtctatatt atccgccacg
     2701 atcttgtggg cgcagaagtc gccgcacatg gtgcagacct tcccgtcagc aggcgccctc
     2761 tcggcccgga tcttctccgc catatccggg tcgagggcga ggcggaactg ctctccccag
     2821 agcatgtccc ggcgggcccg gcccatggcc aggtcctggt cgcgggaacc gttcttgatc
     2881 atgtccccga cgtgggcggc tatcctcgcg gccatcaccc cctccctcac gtcctcgacg
     2941 ttggggaggg cgaggtgctc cgcgggggtg acgtagcaga tgaagtccgc cccggcggcg
     3001 ctggagaggc ttgcgccgat cgccgccacg atgtggtcgc ggccgggggc gacgtcggtg
     3061 accaggggcc ccagcatgta gaagggcttc tcccccgaga ggcgcttcat caccttgacg
     3121 ttcgcctcga tctcgtcgag ggggatgtgc cccggcccct cgacgatcgt ctggaccccg
     3181 aaggcgtggg cctcgtccgc cagctcgctg ttgatgatca gctcctggat ctgggcccgg
     3241 tcggtggcat cgtggacggc gccggcccga aagccgttcc ccatgctgag ggtgacctcg
     3301 tgctccttta agatctccag gaggtagccg aagtcgctgt agagggggtt ctcctggtcg
     3361 ttgtggagca tccaggcgac catgaaggcc ccgccccggc tgcagagccc cccaaacctg
     3421 cccccctgcc tcttgatcct ctcgatgcag atccggttga tcccggtgtg gatcgccatg
     3481 aagttcgtcc cgaggcgggc ctgctcctcg gtgatccgga agaggtcgtc ctcggtcata
     3541 tcgacgccgg acccgtgctt tcttatcgcc tcgatgaagg cctggtagag ggggaccgac
     3601 cctacggata ggctggtatt ctctatcacc cgctttctga tctcgaagaa gtcgcccccc
     3661 gtagagagct ccatcagggt gtcggccccc gcctcctcgg cggccctcgc cttggccacc
     3721 tccatctcga cgtcgacgat gtcgctgctg gtgccgacgg tggcgttcac cttagtccga
     3781 aggccctttc ctatcccgca gaagcgtacg tcccggtagg ggctgacggg tatgacgatg
     3841 gtgccgccgg cgatcccccg ccttatgaac tcgggggtca cgccttcgtc ttttgccacc
     3901 agctccatct cagcggtaat ccggccagct ctggcgtctt ctataagtcc catgaaccat
     3961 cccaccggat ctctcccgag aaggtcatat ttaaagctgg cctgaaaatc ggagaggccg
     4021 gcctcatcct ctccggacga tccttttgat caggacgacc ccgactacgt tcaccgccac
     4081 gatcaggagg actgcgacgt acctcctcgc ctccggatcg aagatcgacg agagaagccc
     4141 tccggccctc ggaggtcggc cggaggcgag gtcgatcgag ccgtccctcg tcaggtggac
     4201 ctcctcggac ctccctcccg actccaccga gtaggtcccc cggtcgaggt tcctaaagat
     4261 gtagcgagaa tcgccggtcg cctttgccac caggaccccg ccccgccaga cctccacctc
     4321 ggccacctct ccggcgtcga cgaccaggtc agatcgcttt gagatcggga tcacccgccc
     4381 ctcggagaat gggagaattg ggcttttgcc gagcaggtcg aggagggtgg gggcgaggtc
     4441 cacctccgac cagttccccg tcaccgcgac ctcgtcgacc cccggcccca cgaagaccgc
     4501 cgggaccatc accgactcgg ccagggagga gtacttggcg gaggagtgtc cgcctttgcc
     4561 ctcatcagag gggaaggaca tgccgtggtc ggcggtgatg aggaggagga cgcctccggc
     4621 ctcacagccc tcgacgagcc tccccagggg tccgtcgagg ccggagatcg tctcgacgta
     4681 ccccgcgggg ccgaggttgt ggccggcgct gtcgacggct ccgacgttga cgatcagaag
     4741 gaaggggcgc tttcccatcg ccgccaccag atccgaagcc gcgtccagag cccaggcgtt
     4801 ataagccaag tagccgggga cgccccgctc ccggttgtag gagggaaaac gatccctcca
     4861 gctctggagg agggcggcca ggtccggagg gagggcgggg ccggagccga ccaggggctc
     4921 ggccccccag agggcgttgt cgtcgaagaa gaggacgccg tcctgctcca ggagcatgct
     4981 gatcgagtcg ccccgctgga ggatggcgag gcggaggagc cccacctcac cagccgcatc
     5041 gaagatcgtc gcccccggag tccccaagat ggtggggtcc atccaggagc agcccgtcgc
     5101 caggaccgag tggctcgggc cggtctccgg gacggggacg gtcaggttca gaattcgagc
     5161 ccccctcccc gtgaggttga agaggcaggc ccgcggcagg ggcgacccgt cgatcccccg
     5221 gggcgagagc tcggggtaga cgtaagccga ccccatcccg tctactacca ggacgacggc
     5281 gccgccggga tcggcgccgc cccccgcccg gacctcggtg gtggggcggg cggggcctgc
     5341 cagggccagg aggagaagga ggacgaagag gaggcgcatc tcagaagtct gtcatcaccc
     5401 tgtactggtc gatctccgtc tgcttcagct ctgagaggaa ctgctcggcg gcgtccctga
     5461 cgtccttgga gccggtgatc gcccggccgg tgacgacgat gtcggcgccg gagctgaggg
     5521 ccgaggcgac ggtatcgacc cggatcccgc cggctacggc gaccaggacc ttccggtcgg
     5581 aggaggcggc catcctcttg atctcggcta tcgcctccca gttgtgccgc tccgcctcct
     5641 gatcgatagc ccggtgcatc tcgaccacgt ccggcagcac cgccagggac ctgaggacct
     5701 ccagggggtc ggggacgttc agcatgtcta tcaccgagta gatcccggtc ttcctcgcct
     5761 cccggatcgc gagctccagg gtcttctggg gggcgagccc cgaaatcacc acggcgtcgg
     5821 cggtggcatc ggcggcgagc ctcacctcaa gatttcctgt gtcgagggtc ttgaggtcga
     5881 ggacgacgaa ggagctgggc ctgacgttcc tgatctcctt caccaccgag gcgccgaact
     5941 tcttgacgag gggggtcccc acctctatca ggagatggtc gctatccgga agctgggaga
     6001 ggaccctccg gacgacggtg aggtccacca ggtccagggc cacctggagg tagggggggt
     6061 cccagagccg gatgacccgg aatcccatga tcgggtgggt ggacctgtcc ttctcgtaga
     6121 ggacggtatc gatgtcaggg aagccctcca tcgccctttt caccgcgagc ttcgtcgccc
     6181 cgtagttgta ccgataaatg gcgtcgtagt cccgggcttg ggggtggatg aagacgctgg
     6241 cgatcaccac caggttctcg acctcggcct tcgggatcac cccctcggcg acggagtcgg
     6301 ccaccgcctt ggcgacggcg ttctgggccg ggccgaatat ggcggcggcc tgctccatgt
     6361 tcttgaccgt caccttcggg acgatcaggg ttgcgggctt cggaggcagg ttcggcctga
     6421 tcaccgaaag gagcggggtg tgccccgccg agagctgggc aaggccgctc gcgaaggccg
     6481 tccccacagg accgtctttg tcaccgatca gcagatcaac atgagcgagt tcgggctcgt
     6541 cccctatcag cgcctctcct atcaaaaaca agcgttcacc tcaaaggcga gagacttcgt
     6601 gcctgtatta atctctttgc acccgccgga agaagccgcc cggctctgga aggccggcgg
     6661 gccacccccc tcagatcacc tgccagtata tgaggggggc gaggaggagg agggtcaaac
     6721 ccgtcacaag cctgattgcg gcccggtggt ccttccggaa gctgtcgacc ctggcggggc
     6781 tcatcccgaa ggcgatcacc ccccccagga ggaggatggg gaggatcacc cccaggttgt
     6841 agaggaggag gtagaccgcc cccgaggagt acccctcctc ggagatcagc cccaggatgg
     6901 cgatgtagac ggcgccgacg caaggggcct tcaccaggga gaagagggcc ccgaggagaa
     6961 agtaagagct gtacctcccc cgggcgaggg ccccctcgac gtacctctgg gaccagctcg
     7021 ccttgaagag ggatcggccg ccggccgata ggcgccttgc gtcctcgata tggacgagcc
     7081 ccagagcgat gaggaggacc gtcaggccgg tccgcaaatg ggcggcgatc gccccggcgt
     7141 ggagggtccg gaggagcccc accccgaaga ggtagtacat cacgaagatc ccaaaggaga
     7201 agaaagccac catcatgatc agatcccggc ggcggccgga gctggagatg atggcggtgg
     7261 caagaaacgc caggaccccc aagaggcagg gattgaaccc ggcgaggagc cccgccacga
     7321 agaccgtcgc catggagagg gaggagacgt ccacctcctc ggcggagccg tcccggaccg
     7381 gggccgtcgg cccgggaatc gaccccacga aggccgggat atcggcgccg gaagctccgt
     7441 ccagcccctc ccaccctgcc ggtgtcgcgg cgagggcggc ccggaccatc tcctccagga
     7501 gcttctggtc cccttcataa tccctatagg ctatcgagag gttcccgacg gcgatcgccg
     7561 ggaccccgcc ccggaggcgg tgctccctta cggccttccg cccctcctcg gagtagacgt
     7621 catagacggc gagctcgacc cccggatggt ctgcgaggat catctccaac gtcctctcgg
     7681 cggcggcgca tttggtgcag cccggcgagg ataccaggac cacctcagta cccgaggcca
     7741 ggggaagaag gaggagcagg gccagggtga ggagagcgag agagagcctc gaggccattg
     7801 aactgatcga catctcggat ccggagaggg ggtatggcat caagggaggg aagacgggga
     7861 gggcgtataa aaagttaatt tggggcgggc cggaaaaccc ccaggccgct ccaacatccc
     7921 cggaggaggg tttaatggtc ggtggtgacg accgtcagca ccgggacgtt cccgtcggag
     7981 tcctccttgg aggcaaagcc gatggaggcg tcgtcggccg ccaccaggac catggagaag
     8041 ggacagtcgg ggcactcatc gatggccttc ttgacgatgc tggtggcatc gaactcgtac
     8101 cacccggagc cgtcgatctc ctcgaccccg tccacctcac cgtcgtactc gggcttgccg
     8161 tcccagacga cggtatcttc gaagaagccc tcgtagtaga agtggagctc cacctcccct
     8221 ggggcctcca cctccttggc atagatcttc agggtcgccg atgcgacctc gtcggaggac
     8281 tcgatcccca gggcctcttt gatgctgttg gtgtcgaagt agagccagga ctctctcact
     8341 ggctcatccc cttcggagga gacccagagg atctcttcgg tgccgaagtt gtcgtcctca
     8401 gcatacttgt cgatgtacgc gtcccccatc aggaccttct cagaggacca gctcgcggct
     8461 gcggccgctc ccaccatcag gagggcgcac cccagggcaa ggcccagttt caacggatat
     8521 ctcatgctga acacctcaat ccaaatatga tatgcggcga taaaaagact ttgtgttcac
     8581 ctctaaccta cgtgagccct acagaggcct cgcctaggcc ggatcagaag atctccgtct
     8641 cgaaggcggg aggagggcca cgttagcttt attaactgaa gatcctcctt aaggccggtg
     8701 ggttagattg aagagttctg cgcatctcag ccccttcctg ggagcgacgg cgattttggc
     8761 gtttctgctg gtgacggccg cctcggcggc gacgatacag gcgggctctg acctccaggc
     8821 ggcgataaac caggcggttc caggcgacac cgtcctggcg cccccggggg tctacggagg
     8881 ggtggagata acaaagagca taaacctcat cggcgacgga gccaggatca gggcggacga
     8941 ccggcagatc gggatgaagg tcggagcgga cgacgtcacc atctcaggct tcaccgtcga
     9001 gggggggttt tacgggatcc acctggtcgg ctcgagaaac tgcaccatct ccgataacac
     9061 cgtcaccggc tgcgtccagt gggggatcgg cctcatctcc tccgacgata acaggatcga
     9121 gaataacgtc gcgaactaca acggcctcgg cggcgaggga tggtacggga tctacctctc
     9181 cggctcccac cgcaacctga tccaagataa cgtggcgagc ttcaacggcg agtacgggat
     9241 agccctcttc ccgagctgct ccgagaacgt catccgggag aacgtggcgg agagaaacga
     9301 ctacggcata tacatgttca ccaactgcgc cgacaacctg atccagaaaa accggctcgc
     9361 ccagaacttc aactccggga tcaagatgat caacggctgc tcaaagaacc agatcctgga
     9421 gaacgnnatc gtcgagaacg gggtcgtggg tatattcctc cagagggggt cgggatacaa
     9481 cctgatccga ggtaacgaga tcgcggataa ctcgaaattc ggtatccaga tccaggaggg
     9541 gccctccggg aacaacacca tcgtcgagaa caacatctcg gcaagccagc gcggcatctt
     9601 cgtcaacacc gacggaaacc acatatacaa gaacaggatc ttcgactgcg tcatccccgc
     9661 cgaggatagg ggggtcaacc agtggtacgc agactacccc gaaggcggaa acttctgggg
     9721 agactacatc ggaaccgacg agttcggtgg ccccggccag aacatctcgg ggagcgacgg
     9781 atttgctgac ctcccccgga tcatcaacga gagggctcgt gacatctatc ccgtcatggg
     9841 cgaggcggtc ctccccctcc agctcgtcga cgcctcgata aaccccgccc gggcatcgat
     9901 aggggccgag gtgacggtgg aggcggtcct cgactcaaag tacggcgtag cccaggtctc
     9961 ggccaggacg aggagcctcc tcagatcctc cgaacggacc ccctacatca ggatggaccg
    10021 ggccggggac ggagcctacg tcggcaccct ccagaccgcc ctgatggggc cggggaggta
    10081 cgagataatc ctctcggcga aggactccaa ggggtacgag gtggaggaga cgatcgggga
    10141 gctggagctg atcccgaggg cgggatggag cttcgacgag gccctctcta aggggcttta
    10201 agataatcat gattcaattc tttcagggga aaaaaaatgt ttggggttag aagaaagatc
    10261 aactcagctg aagggccctg cccgtcggct ggccgcaggg ccgggagtct ccggtcagga
    10321 gcggccctgt gggtcctgac catcgcggcg gtattcgcct tggtctcttg cacgacggca
    10381 gatttggata ttgacgagga gcacgcagga aaatgggact gccccgaatg caaaggccag
    10441 tcgaactcga gcctcgcgat ctttgagttt ttgggctaca atccgacggt gatcgaggcc
    10501 gggacgccca aatccgcgaa acaggcgcgg gccgatatgg aggccggaaa ggaggagact
    10561 gaggatctca actcagccca gagggcgcga gcagagatgg aggcggaaaa ggccgagaga
    10621 aacgctgatg gttacattcg cggcgagttt ctcgcctccg tcgaagacgc gaaggccgcg
    10681 gacgtgatcc tggacgtggg atcatattat gatacgatcc acatcaaggg ggccatcccc
    10741 ctatactggg aggagctcct cgacgacgac aacaatccga agtccgccga agagatggcc
    10801 gagatcttcg ggaacgccgg catatccccc gaggactcgg tggtgatata cggcgactgt
    10861 gagccctgtg atgacatatc cgtcgctccc ttcgtcttct gggcgatgag gtacctcggc
    10921 cacgactccg tccaagtcct ggacggcggt ctggacgcct ggacctccgc cggccagccc
    10981 accgagaggg tggagaacgc gaggcccgcc gtcacctaca ccccccgggt caggtccggc
    11041 ctcatggccg actacgaagg ggtctccagc ggcgacctcc agctcgtcga cgcgagggtc
    11101 ctccgggact tctccgccga taagatcgct ggggccatcc acatcgacta ctccgaggtt
    11161 ttgaccggcg gcaggatgaa ggggagcgac gacctcgacc gggccttctc aggccttgat
    11221 gaggagaggc cggtcgtcgt ctactcctac gccggcgcca gggctgccat ggtctggctc
    11281 gccctccagc ttatgggcta cgaatcgagc gtctactcct tcaacgactg ggaggcgagg
    11341 aggccccccg tcaaggtgat cctggtgagc tccagggcgg accccaaccc cgccccccct
    11401 ggaccggtca agatattcgc caccttcgat gtcgtccccg acgaggcgga ctcgatgccg
    11461 gagttctccg aggcggaggg ctccgatgag gtgacgacga ctgagaccgt cgtggaggtg
    11521 gtctcggagg acgggggcat cgacgatatc ggtccggcgg agaacctatc catcgagacc
    11581 aatggatcca tcgtcagcgg taccgagatc gaggatatca tctccaacgc cacctccatg
    11641 gagaagccga cggctaagac gatgggatgc gtcgcctgct tcgatcccat cctcctctac
    11701 gcctcggggt ccaatccctc agagatcacc agcgggatca agctcggctc catcggcggc
    11761 tccgccctag cccccatcac cgccgccggg gccctgatcc aggactccga agggagggtg
    11821 atggccacca tcgagctcgt ccctaccctc actgacgagt acctggggac ctgggacgcc
    11881 accggagccc ccgccggggc ctacaacgtc accctggcgg cggcgacggg agggaagacc
    11941 tcctacttcg aggacgtcct gacgatagag atcgacggct cggcccccgc cccgacggcg
    12001 cctacgccgc cagcggcgac ggatggcatc agaaagctgg gaaagtatta atcgtatagc
    12061 cacgtaggat gaggagatga gctggcgaag cctctctctt ctcctcgccc tggcggcgat
    12121 cgtctcgacc gcctccagcg gcgccgccat ctccatatcc ttaaagcctc cccaggaggt
    12181 cccgccgccg tggttcctcc agaggatgag ctcggtcctc agccctgaag acgacgccga
    12241 gcctccgacg atccagtacg cccacgtctc tccgatgatc ataaaacggg gggaccccca
    12301 ggtcgaggta agggcctggg tgagagacga cgtaggggtg gagacggtct acgccgaggc
    12361 ggccgggctt tttattccca tgatcgacct cacccggagc ggcaggtacg ccggtcgatg
    12421 cagctcgaac ctcccccctg gagaccaccc gatagagata atcgccgtgg ataaggccgg
    12481 caacgccgca aggaacgccg agctggtcct cagggtcctc gacccccggg acctcaactc
    12541 caaccggatc gaggacagcc tcgaggaggc ggaggagaag gaggaggtga gggtgatagt
    12601 cctcgtcgat gagaacgcca ccctggaggg ggagggcgaa aagttcagga tccttcccgg
    12661 cgcctcgatg atcgttcccc ggggagagct ggagagcatc gccgggacga cgggggtgaa
    12721 gggggtctac gaggacaaga agctcaggat cctgggggag cctatcgagg gatacaggcc
    12781 cgagaacgac cccaggttcg ccgtcgacga ctactccggg aggggcgtta ccgtggcgat
    12841 cctcgacacc ggggccgacc ccaaccaccg gtccctcgac gatatggacg acgacctctt
    12901 cacccccggc gcaaagatcg tcgccttcaa ggacttcgtc aacgggaggg aggatgtcta
    12961 cgacgatcac ggccacggca cccattgcgc tagcctcatc gcggggaccg gggaggggag
    13021 ggggatcgcc ccgggggcgg ggctggtggt ggtgaaggtg atggacagag acggcgcctg
    13081 ctacctctcc gacgccctca ccgccctcga ctggtgcctc gaaaaccggg attccctggg
    13141 gatagacgtc ctctccttca gcgtcggtgg ggaggtgccc agcgaggggg ccaccctcct
    13201 ggacgaggcc tgcgacagga tggtggacgc gggggtggtc gtctccgtcg ccgcagggaa
    13261 cgccgggcct gccaccagat cgatcgtcac ccccggcacc gccgagaagg tgatcaccgt
    13321 cggcgccacc gaccctgggg ggatgatatt ccagaggtct tcccgggggc ccacccccga
    13381 caaccgggtg aagcccgatc tggtggcggt gggggtgaac gtcacctccg ccaaggcggg
    13441 gaccctccgg ggggaggagg atatgagcgg aacctccatg gcggcgcccc aggtggccgg
    13501 ggcctgcgcc gtgatcctgg agaagcagcc cagcctcgac cccgccgggg tgaagagggt
    13561 ccttctgagg tccgccgacg acatcgggcc ctcgggcccc gacaacacct tcggctacgg
    13621 agccctcaac ctctcccgag ccatctccct cgtcgaggag aggcctgcag accccgaggt
    13681 ctacagcctc cagctgagca ggaccgccgc caccccgggg gaccccgtca ccatcgaggc
    13741 gggggtctca ggagatacag ccaccgtgga ggtccacgtc atcggccggg atagggagat
    13801 caggatcccc atgggggacc tggacagaaa cggcgtctac accggccgct gggagacgag
    13861 gttctgggag cccggagatt attcgatcgc agtggaggcg aaggaccctt tcggaggggt
    13921 ggggtctaag gccgtctcct tcgccctgac ttgaggtgga gacctttgga cccgatgaaa
    13981 gagatatcga agtttggaag caagatcgtc tcgtccggcc tgacctacgc ccactttgga
    14041 aacatcagcc tcctatccga ggggatgatc ctgatcacct ccaccggatc gatcctcgac
    14101 gagctgacgg aggacgaggt gatcgccgtc accccggaag ccccctgccc cgaggacgcc
    14161 ctcgccagct gcgagacggt ggtccaccgg gccatctacg agaagacgga ggcccgggcg
    14221 gtgatccaca cccactcgcc ctacgccgtg gccacctcca tcctggagga ggggcccttc
    14281 gagccgatgg acggcgaggg ggcgaagttc ctcggggcgg tgccgatcgt cgagggggag
    14341 atggggagcc gagagctcgc aaagaacgcc tcggccgccc tgaaggggag cagagcctgc
    14401 atcgtccggg gtcacggggt cttcgccgtc ggctcgaccc tcgaggaggc ctacgtcgtc
    14461 gcctgcatgg tggagcacag cgccatgata agaaggctcg tcagcgtcgg atgataattc
    14521 gaggaaggct gatcgggata gagcgttagc aaggcccggc cacccggcgg ctcgggagcg
    14581 gagggagccg atctaaaccc ctcaaaaaag atattaacgg gcagggcctc tccaatacca
    14641 tgagcgccgc cttctcgatc gggaggatcg cggggatccc catcaggctc cacatcacct
    14701 tccttgcgat catccctata gtcgcctaca tcttctcgac cttcaccgcc accacccccc
    14761 tagaggtgct gggccaggtc tacgacctct cgtacggctt tgccgccgtc gagcccccgg
    14821 aggtgcgatg ggcctactcc ttcgccttcg ccatcctcct attcctctgc gtcgccctcc
    14881 acgagctcgg ccactccttc gtggcgatga ggtacgggat aaagatcagg agcatcaccc
    14941 tctacatctt cgggggggtc gcctcgatgg aagacattcc ccggaacccc agcatggagg
    15001 cgaggatggc ggtggcaggg cccggcgtca gcgggatcct cggagccggg acgatcctcc
    15061 tgagcctcca ggccgcctcc ctcctgggcg ggggccaccc cctcaccacc ctcctctgga
    15121 ccctgggggt gatcaacatc atcctgatga tcttcaacct ccttccggcc ttccccatgg
    15181 acggggggag gatcctccgg gcctggtttg cctccaggat gccctacgtc gaggcgacga
    15241 ggcgggccgc ggcgataggg aagatcttcg ccgtcctgat gggtatcctg gggatcatgg
    15301 ggagcccctt tttgatcctc atcgccttct tcgtatacat cgccgccaac gatgaggaga
    15361 aggccaccgc catcgtcgtc cccctggagg gggtgaaggt cagggacatc atgtccaggg
    15421 acctccggac cgtctccccc gatacgacag tcccggagat catgaacctc atgttccggg
    15481 agaagcaccg gggatacccg gtgatggagg ggggaatgct cgcggggatc gtgacgatat
    15541 ccgacgtcca gaaggtcccc gaggagaaga ggggaacgac ggttgtgggg gacgtcatgg
    15601 tgagggcgat atacgtcatc gagccgaacg ccgatgccgc cgaggcgatg aagaagatga
    15661 tggagcggca gatccggagg ctccccgtca tggaggacgg cgagcttgtg gggatcgtct
    15721 ccaggtcaga cctcctccgg gcgatcgaga tctgttcggg gtggtgacgg ctacgggcgc
    15781 agaaagggtt tttagggggg gcgcccctct ggggcccacg gcgaggtaat cgacttggaa
    15841 gagatggtct caggcggctc aatcaaaggg ggcgagggcc cctccgcccc cgggggggag
    15901 aagggcgaga tcatgatcgt ggggacggcc cacgtatccg aaaagagcgt ccaggaggtt
    15961 cggcaggcga tcgaggatct ccgccccgac gtcgtcgccg tggagctctg ccgggggagg
    16021 taccgggccc tcagagggga agaggagacg ggggagatcc agataaagga gatcctcagc
    16081 ggcggcaagc tctacctcct cctggtccag tggttcctag cctatgtcca gaagaagatc
    16141 ggatcggacc tgggggtgaa gccaggatcg gagatgatag ccgccatcga ggctgccgag
    16201 gccgccggcg cgagggtggc cctggtggac agggatattg ccgtcaccat ccagaggttc
    16261 tggacttcca tgagcttcct ggagaaggcg aagctcgtat tctcgatgat ccccgccgca
    16321 ttcgggaagg gggaggatat cgatatcgac aaggtgaccg aggaggacgt cgtctccgcg
    16381 atcatagagg agtttcgaga ggtatccccc agggcagccg aggttctgat cgacgagagg
    16441 gacgcctaca tcgcccgaaa cctcatccgg ctcggaagga cggggagggt cctggcggtg
    16501 gtcggcgcag gccaccggga agggatcgcg aggtacctcg accaccccga gaggatacct
    16561 cccatggagg tgatggccag cccggccagg aggaggatct cggccccgaa ggtcttcgga
    16621 gccctcctga tgctcctcgt cctcgtgacg atcggagccg tcctgatagc cggaaccgac
    16681 agcgcccaga tcatggaagc cctcaagatc tggtttctcg tcaacggcgc cctcagcgcc
    16741 ctcggggtga tcctggcccg gggccacccc ctctccgccc tcaccgcctt catgatcgcc
    16801 tggctgacga gcctcaaccc cctgatggcc gcgggctggt ttgcggggat cgtcgaggcg
    16861 tggaagagga agccgacgat ggcagatatg aagaagctcg ccgaggcgga gaccttcaag
    16921 gagatgatgg ccatccccct cttccgggtg atcctggtcg ccgccctcgc caacctcggg
    16981 agcgtggcgg ggacggtcct aggggcttat ctgatcctcc ggatggccgg ggtgagcccc
    17041 gaggacctcg tcagcgggtt cctttagctc agcacgatac gacgtttatg ccagcgttct
    17101 tgatcttatt cagccggcct atgttcagga ggaggggcgt caacccctcc acctgggccg
    17161 ccagctccag gggcccccca tccaggggcc tcagatcttt tacgacccgg accaggtcgg
    17221 cgacgacctc ctttgcctcg ggatcgtccc caacgatcag aacgtccccc ccaagggtca
    17281 ggtcaagctc ctggaaggcc gcgtaggaga tggtgtgaaa ggccgccacc acccggaccg
    17341 agggggggag gaggtctttc gccaggaggg cggcgctccc ctgggccggc ggtgtgtact
    17401 cgaaatggcg ccccaccctg gccatgggta cgacggggga tatcacgatc tggtccacgt
    17461 agctctccct caggttcgag gtcatcgact cgacgaagtt gtagggcacg ctgaggacca
    17521 ccaggtcgct agcctcgatg gcagcagcgt tgtccatccc ctttatcctg gagtctatcc
    17581 ccctcgactt cagaaagcgg gtcagctccg ccgccgcagc ctcggcctta tcggccttcc
    17641 tcgaccctat gacgatctcg tgctcccccg cccagcgctt gatgaaaccc tggccgcaat
    17701 ctccagttcc tccgacaact gcgatcttca tgtagagcca atctcggatc cattttattt
    17761 tatcatttgc cacgcccccg atacgcccac ggctatggtg gagcagatcg gtgggcattg
    17821 gagcctaagg gaataaggat gaagatgggg ggtctcggac agttcaatat ctagggatgt
    17881 ataaagagga ggataaagag ttaggagatc aattggggat tcgaggaggt ggcagtcctg
    17941 gaaatggtct catttgagat ggaggaaagg gacctacgga tcaatgaagg gttggcacga
    18001 ggtgannggg agtagcaatg aagggggtaa tatttcatca tctcgtgcca ctaagccgaa
    18061 gcgcatctgg ctttggccat cccagactat gtttcttatc ccatattaac tttagggtcc
    18121 atgggaagat gagccgcctc gcgcccccga tctggagggg gtcgcggtga ggacctccct
    18181 cagaacctcg atgggggcat cggtcttgac ccccacgccg ctgaagtggg tcctggaggc
    18241 gctccagccc agggatcgga gctcctcgac gaggtcggag gcggtaccgg gggtcgcctt
    18301 gaatcttctg cagagcctgt ggtggtcgta gtacatgggg aggtcgatct catcggcgca
    18361 cgcttggagg agccggaccc cgcggtcgga ccgggccgcc gacccctcca tcgccgccag
    18421 ggcccttcct atcaccccgg gatcctggat cggccccagc cacagggggc ccgccacctc
    18481 agccctcccg ccgcagaagg agcatttccc cgacgacggg cagcccatcc ctgaaaccgt
    18541 ccgccagagg ccacagctgc gacactgctc gaggtacccc atggattcga gggtcttgtc
    18601 ggcgctcttc gcccccttcg cgatcccgag gtagacccgg acgtaatggt cggtggcgtg
    18661 ggtgaggagg gggcggaccc ccttgtcgac ccgggcgagc tccctcgccg ccgctccgag
    18721 gaggatccgc gcccccatct cccggtggtg ctcggccttg accgggacgg ccatgtactt
    18781 tcggatcccg ctcttgaggt gagctccgca gaggggggcg gtgtccgtcg cggtgatgaa
    18841 gaggtaatcc agggcggatc gcgccgccgc cgccagaaag ggggcggggg agccgaaggg
    18901 gtcgaggtct acggcctcaa acctcctctg gtggaggagg acattggcgt tggacgagag
    18961 gatctcgcac cccacgaggc cgttaagcct cgcgttctct cggatgaggg cgacggccct
    19021 ggggctgacg tcgttgaggg cgacggcggc cgcctccccc tccttcgcca ccctgaggcc
    19081 ccgggctccg ctggcagcga gggcgtccag gtaatctgag atccccaggg ccctgaccat
    19141 cgccaccccc aggtcccggt tcagcctcat cctggggttg tagaaggccc cttcgacgtt
    19201 gagggtgacc gccccctcca tcaccatctc cgccatcagc tcactggtac cccttctcca
    19261 ccagcccctc gctccagtag ttgtcgcatt tggggcagta gtagatcgcc tccaccacga
    19321 tcttcttcca gttctccgag tccagggccc gtctcagctc ccgggcctcg cactcggtcc
    19381 cgcacttcgg gcagaagggc acaaactgcg cctccaagac tacgtcgttc tcctccacct
    19441 ctttgacctc cggaggtaca gatgcgccgc cgccaaaaaa agttgtcgtc ggaccctcag
    19501 aaggcggggg ggcccgaggc gaggaccgcc aggagaaaac cgaggatcgc accgccgttg
    19561 aggaagggga gccccgcctg gggcctatcc ctggaggtgg tggagaggag gaagaagcca
    19621 aaggcggtgc cggccatcgc ccccagggcg gggaggttcg ccgccacccc caagacctca
    19681 acggcgggga ggtcgaggga ccagttggcg gagatcacca ggacggtggg gatgatggcg
    19741 tcccccaggc cgaggaagta agcgccccgg gcctccccgg acccaagccc ctcgcggcgg
    19801 aagctgtacc ttcttcgccg ggggatgacg aagaggaggg gagccctcat ctccatcacc
    19861 ccctccgcca gggtgaccat gtgcctggtc ttgtagaccg agatggcgtc gtaggcggcg
    19921 aggacgacga ggaggaggat ggcggggatg ggggtcatgc tgaggccgaa gagggtcccc
    19981 accccggcag agacgaggag gccgacgaag tcgatgacgt accactccgg atacgtgtag
    20041 aggagggcca ggacggcggc ggagacgaag agggcggcga ggggcggagc aaaggcggag
    20101 atgacgtagt agacccccat cgccaccgcc agcaggacca cccaggtgac gatccagccc
    20161 ttcttcagcc tcatggccat caggatgaag accgtgaacc ccaggacgag gaagatgtag
    20221 ctgaaggggt ttgcggcgga ttcggggtcc tcgaagaccc tcacctccgt ctcggatagg
    20281 gggggcataa ggccgaggga gaggagctgg accgccacca acagggccag catccccacc
    20341 atggggacgg cccgctcggc gaagggctcc ggaggcatat caccgctccg ccaggagctc
    20401 gaggtaggat ttccttatcg attctgagat ccccagcctc gccagaacct cgagggccac
    20461 cctcttctcc tcctcccaga cctcgccgcc ggagagctcg acttcgagga agctccccag
    20521 cccctccacc tcgtccaggc agaggatggc cctccccagg gagtacttcg tcctcctctt
    20581 cttgacgacg gcggccctga agaaccccag ggactccagg atcgactcga gggcctgggg
    20641 gtcgtcgacc tggacggtca cctcccgccg ggacttcgtc tcggggtcga gcttcggccc
    20701 cttgtaggtg aggaaggccc ccgcctcctt gatccggatc ctgagggcct cgtcggtttc
    20761 tgcgaagttt ctggactttg agccgaagta gaggtcgagg tggaactccg cccccgtcct
    20821 cgcgcccccc agtcccgcga tcttctcctc gatccccggg ggcgcccggg ccttcacctc
    20881 cacctcgatc atgtgcccgt ccggatgagg acggtggcct ccgggagggc gatcatatcg
    20941 acggccctcc cggcggagag ggtctcggat tcgatctcca ccgccccggg ggcgagctct
    21001 gcgacctcgc ctccgagatc gacgaggacc ttccccgcct ccgccatccg ggccacctcc
    21061 ttggggtcca tctccctcag cctcctgatg atctcgccgc tcttctcctt gaagacgggg
    21121 cctatcgcct tcatctgcgg cttcacctcc acggccctcg tctcgatctc ggggcggccg
    21181 cgcttcaccg ccaccgtcga gctggtggag cccgcgaggt ccgccgtctc cagagccagc
    21241 tccgagtaga cctcgatgcc ggcgaggggg gcgttcaggg ccatcccccg atcggacttg
    21301 tacctccgaa cggccgccgc cacgtccttg atgagtctcc cctcgggctg gggcaaaacc
    21361 ttccggggct caggccagga ggcggtgtgg accgactccc ctgcgaggct catcctgatc
    21421 tcctcagcga tgaagggggt gaagggagcg aggaggctcg tcagggcctc caacgcctca
    21481 taaatcgtcg cctgggccgc tctcttctcg gggccgtcgg gtccgtacag ccgggccttg
    21541 acgagctcga tgtagttgtc ggcgaactcc tcccaggcga aggacctgat cgccctgaag
    21601 gcctcgtcga aggcgaacct ctccatcgcc tcggtcaccg cctctaccgt ccgggatagc
    21661 tcgacgagga gccaccggtc cacctggttt ggcgatgtcc cctcggagct gacggcgtgg
    21721 ggggcggcga accggtagat cgaccagagc ttctggagga acctgccgcc ggagacgacg
    21781 tccttccacc ggaacatcac gtcgctcccg gggcttccgc cgacggccgc ccactggcgg
    21841 aaggcgtcgg cgccgtactt ctccaccacc tcctcggggg ctatgaagtt gttcagggac
    21901 ttggacatct tgtggccgtc ctcccccagg accatcccgt tgacgaggat cgagtcccag
    21961 ggcctcgtcc ccaccagagc cctcgcccga aggatggtgt agaaggccca ggtcctgatg
    22021 atgtcgtggc cctgggggcg gagctgggcg gggtagcggg gtttgtggtc gctcagccat
    22081 ccggtgacgt tcaaaacgga tatggagctg tccatccagg tgtcgaggac gtcggtctcg
    22141 gggtcgaact cctcagagcc gcatttcgga cacgggaccg gcggacccgt caggttgggg
    22201 tcgaggggga gccactcctc ctccgctagg aggggctcgc cgcaaccccg gcagtaccag
    22261 acggggatgg gcgtcgcgaa gatcctctgg cgggatatgc accagtccca gtccatcgtc
    22321 ccggtccagt tcaagagccg gatctccatg tgttcgggga cccactttat ctcggcggag
    22381 gtctcgatga tctcttcggg gtctatcttc acaaaccact gcctctcgga gaggatctcg
    22441 attggagtct tgcacctcca gcagaggccg acgttctggt cgaggggctc ctccttgtag
    22501 atcgtcccct ccgacctcat ctcctcgatg atggcgtcct tgcactcgga gatcttcatc
    22561 ccggagaacc tgcccgctat ctccgtcatc ctccccgtcc ggtcgatggc ctggcggagg
    22621 gggagggcgt gctcgaccca ccatcggacg tcctgcttgt cgccgaaggt gcagatcatg
    22681 acgacgccgg tgccgaaggc ggggtcgacg acctcgtcgg ctatcaccgg aaccttgtag
    22741 tcgaagacgg gaaccgaaac ctcggttcct atgtactttc tcttcttctc gtcctcgggg
    22801 tggacggcga cggcgacgca ggcggcgagg agctcagggc ggctggtggc gatctccagg
    22861 tctccgaggg gggacccgaa gcggaggtag tttaaggtcg tcgtcctgga gtcgtactcc
    22921 acctcggcaa aggcgatggc ggtcccgcag cgggggcacc agttgaccgg gtggtcttcc
    22981 cgatagatca tccccttccc gaacatctgg acgaaggagc gctgggtctt ggagtagtac
    23041 tccggggtca tcgtcacgta ctcgttcgac cagtcgttgg agaagccgag ccttttcatc
    23101 gtcaccctca tcttggcgat gttctttgcg gtcagctcct cgcagaggcg gcggaactcc
    23161 tctcttggga cctcgttttt ggtgatgccg tggatctcct ccaccttcac ctccgtagga
    23221 aggccgtggc agtcccaccc ctgggggaac atgacgttgt agcccctcat cctcttgtac
    23281 cgggcgacga agtcgatgta gcaccagttg agggcgtttc caatgtggaa gtttccggtt
    23341 ggatagggcg gaggcgtatc gattatgtac tggggcgcgc cggagttcca gtcgaaatag
    23401 tagatcgact cgtcccaggc gccgacccac ctctcttcga tctgcttaaa gtcgtactcc
    23461 ttggagatct cgctcatcgg gatggcctct gagttcattt tttcggcata ggtatgatgc
    23521 tgggaagttg caaacaacgt taataggatt atcggtagaa gataggagca tggatctcct
    23581 catctacacc gccgaccagt gcgaccccaa gaggtgcacc ggaaggaaga tggtccggtt
    23641 cggcctcgcc cggaccacca agcggtataa ccggctccgg ggccgcctcg tcttaagccc
    23701 cttctccgag agggccctct cccccgccga cagatcggag gtggggcggg ggctcgcggc
    23761 cctcgactgc tcctgggccc acgccgagga ggtcttcggc accgtccgcc tcaaggcgag
    23821 ggcgcttccc ttcctcgtcg ccgcaaaccc cgtcaactac ggaaagccct tcaaattatc
    23881 gacggtggag gccttcgccg ccgccctggt gatcctgggt gagggcgacc aagcggatga
    23941 gatcctggcg aagttttcct gggggcacgt atttctggag ctgaaccaag agcccctggc
    24001 ggagtacgcc gcggcgaggg acagccgcga ggtcgtagcc atccaggcgg agtatctatc
    24061 gcaagatatt tgagttttgg agtacgatct actggaggtg atagatcatg acaggattgg
    24121 ttccaagaga cgacttcaag cgcctccaga acaggatgaa ccggctcctg gaggagttcg
    24181 ggatctccga ccccgaggcg aggtacatgc ctga
//
LOCUS       DASM01000140            5289 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_31419, whole genome shotgun sequence.
ACCESSION   DASM01000140 DASM01000000
VERSION     DASM01000140.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5289)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5289)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5289
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_31419"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          877 a         1800 c         1564 g         1048 t
ORIGIN      
        1 gaccggtcga agatcctgat ggggaggtcg tcgctgggaa gggtcctccc ctggaagaaa
       61 acccaggagg ggggacactg ggcgtagcac atcccgccga tgggatcgtc tatcttctcc
      121 ctgatcagct tcaccgggac ctctcttgtc accttgtagt ggtccatcac ctcttcccag
      181 aacttggggt cccgggtctt ggggggagag acgtagtaga tctccgggta cacccccagg
      241 gcgtggccgg acctcttcca tacctcccag gggacgagct tgggaaagat catctctcgg
      301 tagccgaggg ggcctatcat ctcctcgagg acgatcctct cgaaggcccg gaagaggtgg
      361 gtcccctcgg ggccgtagat ccactggccc cggctcgccc cgtgcttgat ccagccccgg
      421 ccgaccatct cctcggtggg gtcgccctcg aacttcgccg gccgttctgg actttgccag
      481 aggagctccc agtgctccgc cttcccaccg tacccctccg cctggatctt ctcgtcgagg
      541 aggctcacta tcctgtcggg gacctggtta cgcaggtccg cctcgtcgac ctccagggag
      601 agggtcaagg tcccgtcccg gtggaccatc tcccggacgt gaggtatctt gtgatcgatg
      661 gagatcttcg attcaacctc gatctcgtac ttagcgacct cgatccccct cactccgacc
      721 ctgtacttct tgcccaggag atcagcgagg ggccgtcgga gccggaggag ggcgtcgtgg
      781 gccctgacgt accggtcgct ctcgatgacg acggatatct cggcctcgcc gaggttccag
      841 ctcaacacct cagcccccat cccgccggcg gcccccttct tcagaaggtc ggccgcacca
      901 ttgaagaact ccccgagctc cttctcggcg gcgccggcgt cggcgctcag cctgaagctc
      961 gcctccagat gaaacttcaa ctccccacct cttcaacttt atcaagcaga tctaatgcga
     1021 tcttcacgtt catcaaaaag tcgtcggcgg tggcgaggat cgtccccgcc ctctcggcgg
     1081 agaactccac cagaaactcc ccgtccccga cgacgacctt catccccggg aggttgtcgg
     1141 gatggaggga cgatgccgcc gcctccgccc cctccccctc aaacttcagc ctcgcccgga
     1201 tcatctcttc ctcccctccg agagctgccg ggccaccgcc ccatcgacgg cagctatgaa
     1261 ctcctcctcc gatccgaggg ggatcgaggc gccgctggcg acgctgtgcc ccccgccgtt
     1321 cccgcccacc gcctctgccg cccccttcag ggcggaagag aggtcgaggc cgcagaatac
     1381 gaggaggcgg ttcccccggg ccgagacctt gacgatcccc tcggacctgt taaggatgag
     1441 cataggccta tcggcgtaga ggtacctgac agagaggccg cagacgaccc ccgcggcgtc
     1501 catctcgttg aggacggcgt agcggaggtt ctccgtctct cgcagccgct cctgcacgag
     1561 gcggacctcc tttaagatct gggccctgta atcccggtcg atcttcctgc actccccgag
     1621 gaaggcttta tccctcatgc agagggtgag ggccatcccc gtctccttca tcttgccgca
     1681 ggagttgagg atccacatca gctcgaaggc gttctctacg acctccctct tcagcctgat
     1741 cgtctctccg acgacggagt cggcggcagt gctcccctgc aggaggagct tgagaaccag
     1801 ggcggtcgag aggcgggaga gctcctttct gccaatcctt ggaagctcgc cggatactcc
     1861 gatctcctcg aggaaggcct tcgccctccc gggatcgccg gtgatctcca ggagggggtc
     1921 gagggacccc gccaggacct ccaccagggg gccgtcgtcc atcttgaggc cggaggccac
     1981 atccaccacc cccgaggccg ccgcctcctc caggatcgtc ctgttcgccc ccaccatcat
     2041 ctgccggtcg cccagggccc ccaccagggc gaggcccgca aggtcgacgt tatcccccat
     2101 ctcccggacg acggtgtaga ccgtccccga gccgctcatc tcgaaggcgc cgtcaacccc
     2161 cgccaggtgg gggttgacgt ggacgcagtc gatatccccg acgggggtat gatggtcgaa
     2221 gacgaagacc tccccctcga tcttggatat aagatggggc tttccgctcc ccatgtcgca
     2281 gaagaccacc ggcggctcga cggagtcgat gacctgctgg tcgagcctgc tgaccagggt
     2341 ggcatgaaag ggtatattcg cccgcctgag ggcggtgcag atcaggcccg cggaggttat
     2401 cccatcggca tcgttgtggg atatgaccct gacattctcg cactccaaga gggagctggc
     2461 ggcgggcttc gccagcttct ccagcctctt catgtgatga gcatctcagc ggtctcgggc
     2521 ctgtactgcc agttctccgg cagcctccca gtccggcggt agtacttggc gagccggcgg
     2581 atcttgttct cagtgagctg gagccacctt ctgttgtgaa ggtccttctt gttgctctcc
     2641 aggtgctttc tcatcccgat agccttccgc atcaggtttg cgaggtcctc agggatctcc
     2701 tgcacctcgg cgttctccct cagatagcgg gtgagcttcg tccccaggat cggctttgag
     2761 ctggggaccc catactggtc ccggagggtg agcccgatca tggtgggggc gacgccgctt
     2821 ccgtggagcc tcaggacgtg ctttcccagc tcctccttgt tcatatcgca ccactcgggc
     2881 ggagttgatc gaaaaggcgg cctcgacctc gaggaaccct ttcttctggt gtgcattctg
     2941 gccatatcga atcctcatta taatcctaag tcagatgaag gcgaaggtaa ccactccacc
     3001 ctaccatgcg ataaatagct tgcggccttg gccgccgccc gtcctccggg gtcagatccg
     3061 atcgctgccg ggggatctga accccgtctt tggaggctcc tgccagggtg gatcggctcc
     3121 ccttatatat acatgccgtc catctctcct tcatgctccc ccccatcatg gacccgttct
     3181 tctacctcct gggaaaggcg gtcttcgtcg tcgccgcagg cgtcctcgcc gccgccctcg
     3241 tcggagccgc cctgatagcc tactccttca agaccggcca cttcttcatg gccaggatca
     3301 tgctcatctc cgtctccctc ctggagagcc ttatcaagag catcttcaga accttccggg
     3361 ccgacgacgc aatcgtcgac gatgtggggg tgaagctccg aaactacatc aacgccgaga
     3421 agttcgaggc ggttcccatg gacgagaggg cgatcttcat cccccagtgc ctccgatccg
     3481 tcgactgccc cgccaagctc acccctgagg gcctccgatg cgtcaactgc gggaggtgcg
     3541 aggtcggccc cgccaagatc ttcgccgaag acctgggcta caggttcttc ctcgcccccg
     3601 gctccagcgt cattaagagg atgatcaaga agtatcggcc cagggccatc gtcggcgtcg
     3661 gctgccccat ggagatcaag gaggggctcg agctctgcca ggggcacgac atcccggcga
     3721 tcggcgtccc cctcctgacg aacggctgcg tctcgacgac cctcgactgg ggccgcttct
     3781 acgaggtcat ctccgaccgg cgggccgacg gcgcccccgg ggaggaggaa gcggagatcg
     3841 ggatcttcag ctggatgtga ccctccggtg gcggatgccg tccccgccgt tgcggaggcc
     3901 gcaagccttg ggcgcccttc tcctccgacg gccttgaggt ccgagcccct gcccaagatt
     3961 tcgccatcag accttccagc cccctcctcc ggaaaccccc tggaaacttc aagcgccagg
     4021 aggcggagat ccccccaggg ggggctccgc tcccgccctc tcctccaggg ccctcaagac
     4081 ctccccgagc cggtcgatct cctggccgta gccggaccag tttccggccc gaagctccgc
     4141 ctgggctgac cggtagaggg accccgcctc ctcgatcagc tcctcggcgg tgagggcgcc
     4201 cgcctccatc cccggtccag ccctgggggc cgccggcgcc aggtcgaaga tcttgctgag
     4261 ggcgatctcc agggtctcct ccatcaccag cctgtcgcca taggctacga ccaccctccg
     4321 cagctccggc agctccccca catccgcccg gaggtagatc ggctcgacgt agaggaggct
     4381 gctatctatc gggatcacca ggaggttccc cctgatgacg gtggacccca tctggtccca
     4441 gagggtcagc tgctcggaga tctcggtgga ctggtcgatc ctcgcctcga tctgcatcgg
     4501 cccgaagacg agctggtcct tcgggaaccg gtataccagg atctggccgt agttcggctg
     4561 gtcgcacttt gcagacatcc aggcgatcat gttgttcctg gtccggggag tgaagggcct
     4621 gatcagcatg aactcctcgc cgacctcgct ctcggggatc ttcatgatga cgtagtaggg
     4681 ctccaccggc tgctgcccgc cctggtatat ctccatcggc gtctcccatg tgtcctcttt
     4741 gttgtagaag atctgggggt cggtcatgtg gtagttctgg tagatcccgg cctggacgga
     4801 gaacaggtcg acgggatacc tcaggtgggc taaaagtccc tccggcatcg agccgatcgg
     4861 ctggaatagg tcggggaaga tcgcctcgta ggcccttacg agggggtcat cctcgatgac
     4921 gtagtaattg accgtcccgt tgtaggcgtc aatgaccacc ttcacagggt ttctgatata
     4981 gttgaaccgc cccgtcggct ccgagtacgg gtagcggtcg gatatggtat aggcgtcgat
     5041 gatccagaag aggcggccgt ccaccagaac tgtgtagggg tccctgtcgt aggcgaagaa
     5101 gggggcgacg gtcctcgccc tttcgaggat gtccctgtgg atcatgatcc tgctttcccc
     5161 cgtcagctct ttgctgagga ggatcttggg gctcatatgc cggtaggcca tcaggagctt
     5221 catcaccgga tcgaggacga cgccgccggt cccggcgtag gtggtgtaga cgttctggtc
     5281 ccccatggg
//
LOCUS       DASM01000141            5267 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_27059, whole genome shotgun sequence.
ACCESSION   DASM01000141 DASM01000000
VERSION     DASM01000141.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_27059"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1042 a         1633 c         1772 g          820 t
ORIGIN      
        1 aagagtacct ccggtccgtc cagtccgagc ggaaggagat cctcaagacg atcggccgga
       61 ggatccaccg ggacgtcgcc tcgaaggacc agtgggtgag gataaccacc ctgggcgggt
      121 gccgggaggt cggcaggagc tgcatgctcc tctccacccc cgagtcgaag atcatcgtcg
      181 actgcggcgt caacgtcggc tccgacgaca gcgcgacccc ttacctctac gtccccgagg
      241 cctacccctt aaaccagatc gacgccgtcg tcctcaccca cgcccacctg gaccatgccg
      301 gcctcgtccc gatgctctac aagtacgggt acgaggggcc gatctactgc acccccccca
      361 cccgggacct cttcgtcctc ctccagctcg actacatcga catcgccggg cgggagggga
      421 agaggatgcc ctacgagtcg gcgatgatcc gggaggcgct gaagcacacc atcaccctga
      481 actacggcga cgtcaccgac atcgcccccg atacgaagct gacgatgcac aacgccggcc
      541 acatcctcgg ctctgccatc tgccacttcc acgtggggga cggcctctac aacgtcgcct
      601 tcaccgggga cttcaagttc gagaagacga ggctcttcga ccccgccgtc cactccttcc
      661 cgaggctgga gaccctggtg atggaggcga cctatggcgg gtcgaacagc acccagccct
      721 cccgaaagga ggcggagggg cggctcctga aggtcgtccg ggataccata aagcggggag
      781 gaaaggtgat catccccgcc ttcgccgtcg gcagaagcca ggaggtgatg atcgccctgg
      841 aggaggcgat ccggaagaag tccctggagg agatcagcgt ctacctcgac gggatgatct
      901 acgaggcgac agcgatccac accacctacc ccgagtacct gaacaacgag ctccgggacc
      961 tgatcttcca caaggggatc aaccccttcc tatcggagtg cttcgtccag gtggagacct
     1021 caagccagag atcgaagatc gtggaggggg acccctgcgt cgtcctggcg acgagcggga
     1081 tgatgaacgg cggcccgatc ctggagtacc tgaaggccct cggccccgac gagcggaaca
     1141 ccctggtgat cgtcggctac caggcggagg gaaccctcgg ccgccggatc cagaaggggt
     1201 ggaacgagat ccccctctcc gccgagggga ggacccagac ggtcaagatc aacctggagg
     1261 tggtcaccgt cgacggattc tccggccact cggaccgaaa ccagctgatg gactacgtcc
     1321 ggagggtcta ccccaagccc tccagggtga tcacaaacca cggcgatgag agcaactgcc
     1381 tcgacctcgc gagctccatc tacaagaagc acaggatccc cacgatggcg ccgatgaacc
     1441 tggagacggt gaggctcgtc tgatccggcc tcgaaggggg acggaccgtc ccctccgccc
     1501 ttttttgccg aatccagcat tttgggcggg ggttatcaat aagatttatc aaatttgagc
     1561 gccaatggag gcgcgaggtt ttgtgcgatg ggaaagagaa ctcggatcat caatgatccc
     1621 tcagatctgg ttcctctgct cctggctttc ggttcagaag ttcacaaacg ggtcttcgaa
     1681 gagctctgcg aggaatggag gacggaggcg gaactcgccg agatcatgag cgacgaaagg
     1741 ggggttcaca ggagcctgga gcttctgaag aagagcggcc ttgtggagac gaagtggaag
     1801 atgccgaagc ccggcgagag cccagagaag gagtaccatt cgagctactc tcgggtccag
     1861 gccaacttcg tatgccccct ggaggatctg agcgagctga tctatctcac ctcgatgtca
     1921 gatgatgagc tccgggagaa gaccgagagg atccagggac tcgtagccaa cggcaacacc
     1981 tccctcacca acctctctcg agagctgaat ctcagccagg ccttcataag aggggctgcc
     2041 aaaagggcgg aggggctggc cgtcaggggt cagaggatcg agctctccaa ggaagagggt
     2101 tgaagaatgg tagacgttct acagagcaag agggacagct cgcggtttca gatcctggtg
     2161 gagatcgccg caaaccagcc gaacgtccgg cagaaggagg tggcggagag gctcggggtc
     2221 accccccagg cgatctccga gtacatcaag gatctggtcg ccgacggcct cgtgatatcc
     2281 gatgggagga tgaggtaccg gatcacgaag gagggggtgg agatgctcct ggagagcgcc
     2341 gccgagctga agcggtatgc taggtacgtc atggaggaga tcatcagcca cgtctcggtc
     2401 tggggggcga tcgccgacgt cgacctcgcg gagggggaga ccgtctccct ggagatgagg
     2461 ggcggcctcc tctacgccgg ccggaaggag ggggtggagg cgagcggggt caccatcgcc
     2521 gaggcccggg ccggggagga cgtgggggtc tcgaacctca gggggctgat cagcctcaaa
     2581 gaggggaagg tcaccgtctg caaggtcccc cgggtccaga tgggcggctc ccggaacgtc
     2641 gacctcgagg ccctcgccag gatcgcccgg gacggagaga tggtgggtgc cctggggata
     2701 gagtccctcg tcgccctcag gaaggtcggg cgggagccag acgtcttctt tggggcgaag
     2761 gagtcggctg tggagtcggc cttccacggg gtatcctcgg tgatcgtctg cgtcgacgag
     2821 caggttccaa acctcatggg gaggctcgag ggcgaggggc tgaagtacga gctcgtcgac
     2881 ctcaccgtgg gatgatcggg tcgagatgcc gatcgccgtt ctggccaggg ccgagaaggg
     2941 ccgggtcctc atcctccccc tccggggcgg ggtgaccctc ttcgaggggc atctccgcct
     3001 ccaggatacg ccgaagggcc cccgccccaa gaagttcctc ataaacaagg acggggacga
     3061 gaggtacctc cagcccgacg acctggtgag gcttctccgg aaggcggggg cgatctacgt
     3121 cgcccggggg gacccctccc tggaggcgaa gttcctcgag tttctcgacg gctatcagat
     3181 cccccaccgg aaggtctcga tctgtcacca ctgcctctcg gagaagagga gcttcacccc
     3241 cctggacgga aaggccgtcc ggcacaaggg cggcctgatc tgcgagcggt gcgccctgga
     3301 ggagctccag agggaggcgg atttccggca cctcggccgg gcggcgaagg tccacatggc
     3361 gaggctcctc ctcaggagga gggacctcga cgaggtcctc ggcctcctgg ccctcgatag
     3421 gctcgaggcc gaccttacta agttcgacga gatcccggcc gagagggagg agaacccgat
     3481 ggccgtcgag gccctcgacc tccctcccga gctgaagagc tttctcttgg ggaggacgaa
     3541 gtccctcctc ccggttcagg cgaaggcgat ccggatgggg cttttggagg ggaaaaacct
     3601 cctggtcgtc tccgccaccg ccaccggcaa gagcctggtg ggggagatgg cggggatcaa
     3661 aaacttcctg gaagggaggg ggaagctcct cttcctcgtc cccctcgtcg ccctcgcaaa
     3721 ccagaagtac gaccagctat ccctctaccg ggacctgggg gcgagggccg ccatccgggt
     3781 cggcgtctcc aggataaagc tcggcaaggc gaagaggatg aaccaggacc tcaactcgga
     3841 gatcatcgtc gggacctacg aggggatcga ccagctcctc cggaccggca ggggcctggg
     3901 gagggtgggg accgtagtca tcgacgaggt ccacatgctg gaggagaagg aacggggcca
     3961 ccgcctctcg gggatgatcg caaggctccg gttctcttac cccgccgccc agttcatcta
     4021 cctctcggcg accgtcggaa accccgccgc cctggccgcc cagctcggct ccgacctggt
     4081 agactaccag gtgaggccgg tggccatcga gcgtcacctg atcttcgcag agttccggga
     4141 gaagaggggg ctgttgagga ggctggtccg ggaggcggcc tccaagacct cctccacggg
     4201 gtaccggggc cagacgataa tcttcaccaa ctcgcggaag aactgcaaca gcctggcgtt
     4261 ggcgataccc aactccgccg cctaccacgc cgggatgcag tacgaccagc ggaggaaggt
     4321 ggaggccctc ttcgccgaag ggaaggtcga cgccgtggtg acgacggcgg ccctggccgc
     4381 tggggtcgac tttcccgcct cccaggtgat cttcgactcc ctggccatgg ggatcgagtg
     4441 gctcagtgtc caggaattca accagatgct gggaagggcc ggaaggcccg gctaccacga
     4501 ccgggggatc gtctacctcc tccccgagcc gggcaagaag taccaggggg ggaagggcga
     4561 ggcggaggat gaggtggccc tccgcctcct ccggggggcg atggaggacg tcctccccga
     4621 gtacggggag gaggagcagc tggaggaggt cctggccaac gccggggtgg cgaagagccg
     4681 ggaggagctg gtccggctcc accggctgac gatcggcctc gacgacctgg ggtcgagcct
     4741 cgccgccctg gaggaggcgg acctcctccg ggggatctcc cccacccgga tcgggagggc
     4801 tgcctccgcc cacttcctca ccccagagga ggtggtggtc ataaggaagg ggctcgccaa
     4861 agggaagggg gccctggata tagccgtcga cctggagagg ttcgatgacc tctacctcaa
     4921 ggccgccgag aggatctcgg cgaccctgag gatgcaggtc tcctcgaggg cgctccacgg
     4981 ctcggtgatg gacctcctca gcagcgaaga cctcaccaag ctcgacccga agctgaggga
     5041 ggagctcttg agcttcgcca aagatttcac ccgctgcggc tgccgggact ccccctactg
     5101 cggctgccct gagagggggg cgtccctcaa gatcctggag ctccgggccg aggggatgag
     5161 cccgagccgg atcatccagg agttcaccga ctcttacggg atctacgcct acaccggcga
     5221 cctgataaac tatctggatg cgatcgtccg ccggctggag gcgatcg
//
LOCUS       DASM01000142            4634 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_119676, whole genome shotgun sequence.
ACCESSION   DASM01000142 DASM01000000
VERSION     DASM01000142.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_119676"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          918 a         1310 c         1451 g          953 t
ORIGIN      
        1 cccactcgat cctctttttg cccttctcgg ccaacttcgg gtccttgatg acgttctcca
       61 tcaaaatatc accttgatcg atcttgagct ctgataatgg cttttgggat cagattcttt
      121 gtatatactt aaaaccgatc ggtcggggct gtcggcaacc tcaagtctgg gccatcgaca
      181 acctctatta cttcgggatc ccaaaagcct ctcatggacc tggaggtcac ccttcttgga
      241 accggggtcg ggatccccca ctcgggccgg gcccaggcgg gccttgtgat cagggccgag
      301 gaacctatct tgttcgactg cggcgcggga attttgctga ggctggcgga ggcggggctc
      361 tcgcccctgg aggtcgacct cgtcctcctc acccatctcc acctcgacca cgtctccgac
      421 ctcctcgccc tggccaaggc ccgctggctc ctgggcgagg acaaaatgga ggtcttcggc
      481 cccgagggga cggcagggtg gtttgagacg gtccggggtc tttaccctta cctgaaagac
      541 ttggacatat ctttcaccgt cgtaaatccc ggagatgagt tctgcctaaa cggcctgagg
      601 atcagggcaa gcgaggcggt ccacagcctt ccggccctgg ggtaccggat cgagtcggag
      661 gcgagggcgg tcgtctactc cggggatacc gagccnncct tcgtcgtcac caaccactcg
      721 acgccgaaga agctcggcga ggccttcagg ggcgcgggcg taaatcggat cgtcctcacc
      781 cacctctacc cccagaccat cggccgggag gcggagatgg tccgggacgt cctggcgggt
      841 gtgggcaccg acgtccacgt ggagatcggg cgggatctgc agagaatatc aatttagttt
      901 ttgggcggag aagagatgat cttcctctag gccacgaact tctggtagca ctcctcgcag
      961 acgatcttgc ctcccgccgt ccgccctcgg acctccatga aggcctcccc gcactcctgg
     1021 cagtcgaggg aggggtggat tcgggccttc ggcggcgcgg ggatatcgac ctgtcggacc
     1081 accaacagat cctcgtcggg ggcggtgagg atgtagtttg tagcctcctc ccggagcttc
     1141 aggaacctat ccctctcctc gggggtcatc tcccctgcga agaacctctt catggcggcg
     1201 gggtcggtcc gggggaggac gtttttgaag tagatccgaa tcgccttatt gtggtctcgc
     1261 gagtagaagg tgtagacgtg ctttccgtgg tccttgaaga tgaagttccc cttcccgaag
     1321 gtgcaaccga ggagagactg gatggcgtcg acgctgcagg agctgttctc gacgatcgcc
     1381 accagctcct cgtcctcgga cctgctgtaa tggttcttga cgtacttggc cacccggtag
     1441 cctatggcga tgccagggca ggtatggccg tgaaacttga ccacttcgtt gaaatcctct
     1501 tccatctcgt caccttgttc cgaatttatc ggatcgggtt atgatctgat actaaataaa
     1561 ccgtatcgtt tcaaattctg atcagcgtcc ttccgagggc ctggaggagc ggggtggggg
     1621 agcttccggg tcagaggacg gatttattcc ccaggaggag gattgccgcc gcaatgggac
     1681 tgaccaatct gagaaggagt ttgggaggcg ccgagccgca agtcgccggt cttttatccg
     1741 acgctcccat cacggagact gccgaccttc ctggtcttct gctcgcgcct cgataataac
     1801 taccacccca ttctatttat tttttatgca ggaggcgtcc ggcagaagct gtccgggccg
     1861 gagggggtca ggtcatcagt tattttaagc ttgaggggga agtcgcctcc atggagtttg
     1921 ctttggagat cgtcaaggcc gataagttca ggcagatcta cgccgtgggg gcgatgggag
     1981 gccacagccc ctacgacttc aggatggcct tctacaacga ctcgccgaag gccgtagtcc
     2041 aggacggaaa gcaggtgagc gtcatggaga ggagggtgga gacggagctg atcctctctc
     2101 ccctcgccgc aaaggagctc gccgactggc tgaataacca cataaaagag tacgagcggc
     2161 agttcggcga gataaaaaag ccggggggga ggaaggccgg accggaagag tccgctccca
     2221 ttcagggcta catgtgaggc aggtctcatg agatcaaagg agaagatcct cgtccacaag
     2281 gggatggatc gggtgaagag gaacgagttc gaggaggcca tcggctattt cgaccaggtc
     2341 ctggagatga accccgagaa cgccgacgcc tggaacaaca agggcgtcgc cctcttccag
     2401 ctgggaaggc cagaggaggc cctcgccgcc tacgaccggg ccttccaggc cgaccctgaa
     2461 agccgtgagc ccctcaggaa ccgggccttc gtcctccgga cgatggggcg gttcgaggag
     2521 gccctcgaag tatacgaggg gatcatcacc gaagagccgg aggcctccga cttccggaac
     2581 ctcgccaccg tcctggtggg gatgggcctc ctggaggagg ccctgggagc cctgatggag
     2641 gcggcgaata tcgagccgtc gatctccatc gagagggaga tcgaggccct gaggcgggtg
     2701 atgatggagg tggcgatccg ggcggagttc ggtggcgtcc ttccggggga gggaggcggg
     2761 gcggagcggc ccggcgaagg atgatatagg atcggtgtta catataaata aatctccgaa
     2821 gaaagggggg gttataggtt gaaaggtttc gtcatgatca acgcagagcc ggggtccgag
     2881 aaggcggtat acgacgccct caccgcgatc cggggtgtcc gggaggtggt cccggtctac
     2941 ggggagacgg acttcatcgc catagccgac gtggagggga tcgccgacct gaatatgata
     3001 gtcctcaagg tccgggagat ccacggcgtc acctccaccg agacgatcct ggggatggag
     3061 ctgaagtttt agctcccccc gcaggcgagg ctgccctcag gatcggtcag cggggggggg
     3121 ctggcccgag aggagaaagc cttcggcggc tgagatctct tttccctccc ccggtccgtc
     3181 cccttcccgc ttcttttatc ggcgccagga gttcgcttct gccctccccg tctcttgagg
     3241 cacccctggg aagggcccct cgccccccat acgatcctgg gcatccggcc gggatgggca
     3301 cggaggcgaa tggcggaggg cctccgccgg gcatatcgat ggggatcgat aagactggga
     3361 cgatgatggg tggggcgaca tcttcgcggg ggtgaaaaaa ctcggcgatt ctccgtcttt
     3421 acatcggatt cagatgaagg ctacataaac atgaaattta aatatcgatg cccgtataga
     3481 tgctgacata gcagtatcga atctaaaggg gagatgaggt atatgaagcg aagcagagtt
     3541 cctaaattgg tgctgatctt cgtcctgctt tctttgatga cagcatcaat ctgcattgcc
     3601 gccccgggcg ggcatccgcc ggggccgaaa cataaggatg ggactcaccc tccgggatac
     3661 cacccgccgt attcccaccc accaccgtac cacccgccag cgtatagcca gccggtctac
     3721 tactacacca ggccttatgt aacctcctgg aattacgtgg tctcttggag ctactggagg
     3781 ccgtggggtt acagctggcc ctggggatcc tactggtact ggtgatctct gaagggccat
     3841 cccggggatc ggcagggacg aaacccctct tccggcggga gatgaaagct ggggtcaggg
     3901 ggctagcccc cgccgcccca tcctttttat tgaagatctt tacgccaccg gccccgaaag
     3961 gaagataaat tcttatcttt tgaacgcatc aactcctcgg ggaggacctt cctcctttac
     4021 aggatcagca ttatccgagg gatataaaag gggatgcagc ggggatgaag aagatcatat
     4081 tctcgatagt cctcatcatg gctttgatgg ggtgtgtcgc agccatcgga ggagacggcg
     4141 gcgttgggat gcggccggga gaggccgcgg ccgaagagcg gttcgagcga ggtttgggag
     4201 gcccgtgggt ggggggcgat ccctggccga ggggcgatct gtcgaccggg cagtcattcg
     4261 gccatagcca tccgaagttc tactcgatct tcaggccggg gttccccttt tcgggatacc
     4321 actactggac cggggttcac atccggccct tccgggtctg ccatccgttc agcgactgcg
     4381 actgccagtt ctattatcct cgaaactact attccggatg ggtattccag ggttaactcc
     4441 ctcgaaggcg atctatgatc agaccgcctc aaattctttt tgcttctggg gagccgaaac
     4501 cgatatgacc ggagaggtcc catcagcctc ggatagccca tggccaagat ctcacccctg
     4561 atggatcagt acttcgggat gaaggaggag tgcggcgacg ccctcctcct cttccggatg
     4621 ggcgacttct acga
//
LOCUS       DASM01000143            8246 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_134312, whole genome shotgun sequence.
ACCESSION   DASM01000143 DASM01000000
VERSION     DASM01000143.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 8246)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8246)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8246
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_134312"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1497 a         2688 c         2389 g         1672 t
ORIGIN      
        1 atggtgtact ccgcgacggt gatcctcctc tcggccatct cctcgacgac cgtctcctct
       61 cgaaccaccc cggactttgt caggttgata tgcggccgat cgtacttcga gaccccatcc
      121 aggaggacct ttcgccggat cgagtagtcg ccggcgtata gctcgtccag gtcgaccctg
      181 tctatgaaga cggtcctgta cctcccccgg cccgagaagc tcgcctccgt ctccatctca
      241 ttcaagcccc gggccaccgt ctccttatcg agcctggtga tggaggtgta ctcttcgccg
      301 atgaggctct cgtttctgac ccgggaccac atcccctcct tccacctcat ggaccggttt
      361 atagagagat cgtcgatgag ggtctggttt tctgggctgt agacgaggct caggtccttg
      421 gccacgtagt tggtgtaggt tctgatcgtc tcctccgact cgtaaaagcc agtaccagac
      481 tcgtaggacc tctggctctc tccgatccgc ttgtccacca ccacaaaccc ggtccctgaa
      541 gctgaccggt cggaggagac tccggagccg tactcgtcga tcctctggag gacccggaag
      601 cttccggcgt aatcctccct catctcgaag atcggggtcg accgggggcc cgagccgttc
      661 gaaggcatct tgaggaaccc catctgcccc atcccgtcga actccgattc gacctccatc
      721 accgactcgt tccggtcaag gaagaacctc gattcccggt ctatggaggt ggcgtaccgg
      781 taggcctcgg ccatcgaggc tccggtcgcc cggttcttcg ccttcgcctc ctcggtccac
      841 cgggacgagt aggcgagggt cctgttccga tagagggtca gcgtcgaagg accgtgatcc
      901 gccgagacgg ccttattcat ctcgatggac ctgttatccg aacgaagggt gatcgtctca
      961 tcgctcttgt agaggccgct gccgtgctcc tgggtggaga gctcggcgcc ggggtcgtcg
     1021 atctggacgg cggcgcagcc tctctctctt cccaggatga agccgttcag atagaagacg
     1081 gccgtggcgc agtttttgag gatgtaagaa ggcggagccg ttgagtaatc gctcgccacc
     1141 ctgacaaacc ctgtgccagc gacctccgat cccatctgga actcgaactc ctgcctcttc
     1201 ggcgccttca ccaccaggac gatctcccgg cactcgccga ggccgatctc atagaaacgc
     1261 cagaggccgt ccccgtcggg ggctggggag acggatacga gctccaccgg cccatcgaag
     1321 atgtcctcga cgacggcgtc tacggggacg gtgctgtttc tgttgcatac ggtgatggtg
     1381 taggtgatct cctcgccggg gctggcgacc ttgcggtcag cgaccttgga gagggctttg
     1441 ttgtcacagc tcagatcgta gaattctatg ggagacatca ggggatagaa atcccggtca
     1501 gatcctccag gaatatcgta aggtgtgtcg ccgatgccgt cgccgtcggc atcaaccccc
     1561 gaatagtcgg accagtaatt gcccagacgc ctggtgaagg tggtgccgct gtaccggtag
     1621 gtcatctcct ccggcgagtt ccaggtattt tgggaatttg accagccgac cgggctcgat
     1681 ccattgatga aattgttgag ataaattatg ttgccgttgg aggattccag gcggatggca
     1741 ccctgacgat tctcttctgc cgtgttacct gtgatgacgt tgccgctgga acttctgagg
     1801 aggtacccta ctcctctatt atttttggca atattgtcct tgatgacgtt gttatttgat
     1861 aggactttaa tcccagcctc ggatcggtcc gaatttatcg ccaccaggcc ctctatgagg
     1921 cacccctctc cgctgacggt gatggcgctt gtgacaaatc ttcctgtaaa ggtgggcggt
     1981 ccgtaagtgg cgtccatgat cacgccccgc aagtataggg acttgtttac gtcaatctca
     2041 gtctggttag ggtcctgtcc gtacggagaa ctcgggtcaa tgccccgcac ctccagggta
     2101 tctccgttct gggccctggc gataacatta cggatggcgt acatatcctc aatagatcta
     2161 tcaggccccc cagcagagag gggtatggtg atgaaggtct tcccccctac ttcacccgag
     2221 agcgccgaag gtggcgggac gacgtagatc acctcccggg agaactccgc ccccctcgcg
     2281 aggatcaccc tctcgatcag cgcctccagg atctcgtgat cctcgtcggt gaggctgtac
     2341 tccgtcgaga ggattccggc ggcctcggcg tggtttgtga tgtttccaaa ggtggtgccg
     2401 cttatcctgg ccaaaagctc cacccgcata gactcgccga cagagaggcg gcccagctcc
     2461 caggttattt tccttccagc aacggtcccg ttccggtcgt ctcccaggta ctccaggccg
     2521 tcggggagga cgtccgccac cacgacgcgg tcgaactcca tctccccgag gttcgccaca
     2581 tcgattgtga acctgacgac ggctcccggc ccccccgagg tgacgtttcc ggccttgtcg
     2641 agatcgatct cgggctgcag tcccgccatg atcagggggg cctcatccct ctcggccccg
     2701 aagacgtggg ggaggtcgcc gatcccgttg ccgtcggtgt cggggccgga gtactccgac
     2761 cagtggtttc caaggatagc ggtgaaggtc tcgcccctgt acctgtagcc gatggggtcg
     2821 gaggagttcc agcggctggt ggagttcgtt gatgaggcgt ctattccgtt atctttgaag
     2881 acgttgagga agagggcgtt ctcggaggag ttaaaaaggc ccgctccgca ggggttatcg
     2941 ctaaattcgt tgtgggtgac gatgttgtgg ctggagctat cgaagaggat gccgcatcct
     3001 gcgttctcct tcacgacgtt gtctcggatg acgttcctct ccgaagtcac ccggaccccg
     3061 gcgtcttcgt ccgtcttgct cccggagttg gtgacgacaa acccttccac taccgccccc
     3121 gagccgctga gggtgatggc gcttcccctc cccccggcgt ccaccactgg ccttcccccg
     3181 ccgttatcga ctccgaagag gatcagaggc ctgtccacct tcacattctc acggtaagtc
     3241 ccgctgggta cctcgattat atcgccgggg tcggctccgt cgatggcggc ctggatgctc
     3301 tcggaaggtc ccaggactat cgtccttccc tggacggtgt atcctgagct gatgatcatt
     3361 atcgccatcc aaagccagaa atacttcatc caaaggctgt tattgagcac cctctaccac
     3421 caggacatct taggctgtta tctacaatcg tgatattaga taaacttttt cataactttc
     3481 ccattctttc aattaatatg aaaatattca ataacatatc tttgtgatgc agcctccagc
     3541 ttgaaggatg aagtgtgaat caatctgttt atatccaagc aaaatttacc ggctaaaaga
     3601 acagatcgct ccccaattac caattatatt caacttaata ataaaaaaat ttataactat
     3661 gtataatatt tcgggataca ttaatgaaca atatattaga agtattgcta gacatataat
     3721 aatatatcat ggcatatact aatgttaaat aatgttggag ttctctccat cctccctttt
     3781 aggcttctgt ctgggtcaac ccctgccaac gatcatcggc tttaatatca tggcgagatc
     3841 cgatccgcca tctccgtcat cctgaagctg ccgtcgagga tccgcccctt cagctcctcg
     3901 gcgagcctct cggcccgcag ccggtcggac tgccgcacga cgatcggaac ctcccgcccg
     3961 gcgaagcgga gggatccgag gcgggctctg aagacgtctc cggcgcagga ggtggcgcag
     4021 aggccgcagt tgaagcacct cctccggtcg agggtgggaa gaaagtttcg aaaggctatc
     4081 gcccccgtcg ggcagatcgc ctcggggcgg caggcggtgc acctcttgca ggccccgggg
     4141 tcgaagctga cctccaggtc taccccctcc cagacgtcgg cgtaggtcgc ccggcccagc
     4201 tcctggcggc ttgccacgtc caccaccggt agcggtatag acccttcgag gccggccatc
     4261 ctctccagga tcccctccct caagaccggg atcgggaccg cccacgacga tatgcactcg
     4321 gggcctgcgg atgttccgaa gcctcccatg tactcggggt ccatcccgtg catatccgca
     4381 taccccgaga ggctcggccg atcgggggag gacctcgtcc ccctgcccag gacgaacccc
     4441 tcggcaccgt tgatcaggac cctcgtcccg accccgatcg tctccaggag ggggtcgttc
     4501 tccagggggt tcatgcggcc gcaccccgag acggaggcgc cgttccagca ggggtcgaag
     4561 gtggtggcgt ggaagatggt ggcgaccctc tcctccccgg ggttgacgaa ggccgagtag
     4621 ttcttgaaag agtggcgggt cccaaagagc ctcgccgtcg gcatatcggc gagcttcagg
     4681 tccacctcga agccggagcc cgtgtccgtc tccacctcga cctggacctc ctccccctcc
     4741 accaggtccc ggaggaggtg gcctccgccg tacctcggct cctcccggct cgccgccgtc
     4801 ccgaagacga tcaggtcgac gatccccagc cgctcgttgg ggcagggtcc gacgatcccc
     4861 gggacgccgt tgagcgtgac cccgatcgcc cgccggaagg agccgggggg cgccgccgga
     4921 aaggagagga cggcgtaggt gccgctcatc accgcccggg tggcgcaggt gacgacgtcc
     4981 acctcctgta aagagaccct ccccccgtcg gcgaggacct cgctcacctc ccgggcggtg
     5041 aggacgacgg cctcccctcg gtccaccttg gctctgatct cggagaatga acgctccatg
     5101 atgcctcctc agccctcttc cttaatgaac tccgcctcca cctccgaccg gagatggtcc
     5161 cgccccatga tccgggttac ccttctatcg gtcaagatag agacaggccg gagcatatcg
     5221 aagagctcca ggccttcggc ttcggtgaag tagtgggtga tcaccccccc gccccggcgg
     5281 aaggtcatct cctccacctc caccccctct cccgccctca tatcctcgac ctcgaactcg
     5341 ataaagaaga ggcgaccttt ggccttgagg acccgggccg cctcccccgc cgccttgatc
     5401 cggtcgccct cccggaggtg gccgaggacg tggaaggcga agaccgcatc gaaggacgag
     5461 tccctgaagg ggagctggac cgcgtccgcg gcgacgaatt cgagccgatt ggccggaggg
     5521 acccggccag ccggaggtgc tggcggcggc gggacgccgg agggggtcga aggctccggc
     5581 cggaggtgat ctcctcggcg gcacatctcc tccgccagcc ccttccccag cctcagggcc
     5641 tgaggagata cgtccaccgc cgccaccctc cagggtcggc ccagcatccc ctggagggtc
     5701 ttgccgttgc cgcagccgag ctccaggacc cgggctccct cggggagggg ggggagcctc
     5761 cggggagccc cccgccagat catcccctta tctcgatact cccgccccca ggctaggagc
     5821 tcctccctcc ggctccctcc attcccggat ccgaccttcc gtcccggcgg catgaggtcg
     5881 gaacggcgct ccaccaccaa aatctttact atcctccagg taatccccac ccaggtgttt
     5941 caaatgagac ttatgagtgg ggcaattctg atcattgcca taatcggctt cctgggcttc
     6001 gcccaggccg acggcgagaa ttatgaggat tatttcggtg ggtcctccct gggggaggac
     6061 tatctgaact acgccgggca gctgtacgga gcctccggct cctcggagag ctacagcttc
     6121 agctcaagct cgagcttcac ctactcccgg tacctccagt acttcttcac cgaggaggat
     6181 atcttctccc gggatcgggt tcccgtcagc ttcgtgatag caggaaacga gccctccatc
     6241 ctcgacctgg ggtggcagac ggtcccctat tcggagtacg cctcctcgga gatgtacgcc
     6301 gggtccaatg agctctggat cgagggggag atggcctggt cgaggtacgc cgcctgtccc
     6361 atcggtacat ggctgcagct cgtcaccacc acccccgggg gaggggccgc cgacttctat
     6421 gagatatcgc cttcagggat cgtggggata accccctaca gcctgggaac ctacgacagg
     6481 atgagcttcc aggccgctga gatggggagg tacgccctct tcttcgtcgc caacggccag
     6541 ccgagcaacg tcgtcatcat cgacgtcgga gaggagctga ccccggatat gatcgccggg
     6601 tccgctgccg cttcaactgg gaccccgggc cctgcccccg ccatcccggc gccagctccg
     6661 aggcccgtca ccaccaccgg tgacgtcgtc gtcaccctgg agtccgaggg gatgaggggg
     6721 tacgacgtct acgtcgacga ccagtacgtc ggtaccgacg ggcagggggg cgaccccctc
     6781 gacgggagat acagggtcaa cgtcgccgga gggatgtggc acaccatcaa ggtctgggac
     6841 ggggagtggt tctacggcaa gccccggttc taccagagga acacccccgt cgtcctgaag
     6901 ttcgagcccg cctccaccgt ctacctctac ggcgggatgt ggtagccgga tcgggcttta
     6961 gatcccttaa tttttttctc cctcgccctc accgccacca tcttaaggga ttggagacgg
     7021 aatacctaac atgaagggat gggggctcgt cttgttgttg ggagtgagca tcctagccgg
     7081 atccggccct gccgggggtt gggtgagcgg aacgggagag atcgacatca gtagatccgt
     7141 cgtccagagg atggcagatt acgagtgcca cagctccatg gccggagacg ggagcctccg
     7201 gattgggggc agctcccccg gggcttcaag cctctcctac tccggggacg tccccctggt
     7261 ggggttcaag cggatagaga ccggatcgcc cttccgtgga acgaagagct cctttcagga
     7321 ggccttcagc gccacggata tggagcggga ggtcacgacc tcggcgagct ctcacctcgt
     7381 ggccaccgat atgaagctgg ccttcaacgg gacctacgtc accagctcca atatgcatca
     7441 ggccttcgtc cgggatgtca gctcccatca gaggtacacc ggaaacttcg agatacagaa
     7501 tatgatccag ttcgggagcc tcgccaaccg gaggccggcc ctctccgtca ccgtcctccc
     7561 ccaggactgc tccgcccagg tgggagggcc gattaggaga gagtacgccg tctccaacgt
     7621 cgggaccgtc cccgtccggg acctctccct cgtcgattcg agggtcggac ccgtccccct
     7681 cagcaggacg gagctgagcc ccggggaggt cgcctccggc tcctcgagct tcgccctcgg
     7741 cgaagaggac gtccccggcc tccataagga ctcgatcgtg gcgaccgccg tcgactacgc
     7801 tggaaatggg gcggaggcct ccgcctccgc cacggtggac ctgatcgggt gggaggggct
     7861 gaacctgacg gtggagtcca gcgatggatg catccatccc ggcggcggcc tcgcctccgt
     7921 ttataccatc gagaacaccg gcgaccttcc gataaataac ctcaccgtca ccgactccat
     7981 cgcccccggt cccgtcgctg cgaacctcac cctcatgccc ggagaggccg tcaacctcac
     8041 cgccgacctc gcggtgctgg ggccttctcc cctgacccgc ctggtctccg cctcggggaa
     8101 ccgatccgat ggggagatgg tggcaaggtc gatagaggtc gtccttcagc cctgctgaag
     8161 gcctccgccc ccggctccct ctcccccctc ctcttcattc cagcgattcg atcctttcga
     8221 tgtaagatcc gacgtcgaac ttcgac
//
LOCUS       DASM01000144            6840 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_43902, whole genome shotgun sequence.
ACCESSION   DASM01000144 DASM01000000
VERSION     DASM01000144.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_43902"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1194 a         2237 c         2134 g         1273 t
ORIGIN      
        1 gagtcccgga ggttctgggc ctgggcgtgc ccctggcttc cgtaaccgat gatggcgatc
       61 gtcttcccct tcaggacgcc caaatcggcg tccccgtcgt ggtatatctt tgccattttc
      121 tgtagtcctc cctatcttga gaagtcctct tctgagaatc cattaagcat taaatataaa
      181 cttgatctgg ttcgtgattg atgatcgatg atccgtccgg tcccctgggg gagacccttc
      241 gttcggcccg ggtccgatga gggccgggcc gaaccgatca ggggttcacc accttcgctc
      301 ccctgatcat cgagaccttc ccggtccggg ccatctcctt gatcccgaac tggcggagga
      361 gccggaccat cgcctcgacc ttgtcctggc tccccgtcac ctcgattatc atcgatcggg
      421 gagatacgtc gatgatcttc gcccggaaga cgctgacgat ctgcatgatc tcgcttctgt
      481 cctccttctt cgccgcgacc tttatcatcg ccagctctcg ctccaccgac tccgcagggt
      541 cgagctcgct gacccggatg acgtttatca gcttgttcag ctgctttgtc acctgctcga
      601 ggacgacctc gtctccgtcg acggtgatcg tcatcctgga gtagtcgggg ttgtccgtcg
      661 ccccgacggt taggctgtcg atgttgaagc cgcgcctgct gaagagcccc gctatcctca
      721 ccaggacgcc gggcctgttc tcgacgatga gggctatggt atggagcacc ttcacccctc
      781 cagctccaga atctccgcgg cagccgatcc tgcatacgcc ttcctggtcg tgccaggctg
      841 gtccgccctc tccagctcca ggatctcgga tagggaggcc cccgccggga ccatcggcga
      901 gaccttctcg tcgggggata tgatgacgtc gacgacggta accacctctg aatcgatcgc
      961 ctccttcagc acaccctcca gctccgaggg cttcgtcgcc cgcaggccca gggccccgta
     1021 cgcctcggcg accttgacga agtcggggac ccggtggagg gtggtgctgg agaacctctt
     1081 ctcgtagaag aggtcctgcc actgccggac catccccagg acgccgttgt tgaggatgat
     1141 caccttgacg gggacctcgt tgaccacggc cgtcgccagc tcctggatgt tcatctggaa
     1201 gctcccgtcc ccggctatgt cgaatacatc tgcatcgggg cagccgagct tcgccccgat
     1261 cgccgccgga aatccgtacc ccatcgtccc cagccccccg gaggatatga acctccgggg
     1321 atccttgtgg gtgaagaact gggctgccca catctggttc tggccgacct ccgtcacgat
     1381 gaccgcctcg gggcagagct cgtagagggt ctccaggacc gcctggggct tgatcgtctc
     1441 ggagtccttg gcatagccga ggggatgttc tcccttccag ccggaaatct tgtcgttcca
     1501 ggggctccag ggcttatcct ccacggcctt ggccagctcc gttagggcgg acttggcgtc
     1561 cccgacgacc ggtatgtcca cccggacgtt cttgccgatc tcggcggggt cgacgtcgac
     1621 gtggatcacc ctcgcctggg gggcgaagct cgccaccttt cctgtgaccc ggtcgtcgaa
     1681 gcgcgccccc acggccagga tcaggtccgc ctcctggacg gcgtagttgg cgtacttcgt
     1741 cccgtgcatc cccagcatcc ccagggagag gggatggtct tcggggaagc agccgatccc
     1801 catcagggtc gtcgtcaccg gggccttgat cttctcagaa agccggagga gctcacgatg
     1861 gccgtcggag tacttgaccc cgcctccaac gtagatgacg ggcctctccg cctccatcag
     1921 ggctttcgcc gccttcctga tctggaggtg gtggaccttc aggttggggc tgtagcccgg
     1981 aaggttgatc tccggatact tgaactccac ctcgtcggcg gtgacgtccc ggggaaggtc
     2041 gatgaggacg gggccgggcc ttccggtccg ggcgatgtag aacgcctccc tgaagacccg
     2101 ggggatctcc ttcgcatcct tgacgaggta gctgtgcttg gtcaccggca tagtgatccc
     2161 ggtgatatct gcctcctgga aggcgtccct tccgatgagg ttcgtcggga cctggccggt
     2221 catcgccacc atcggcaccg agtccatgta ggcggtggct atccccgtca ccaggttcgt
     2281 ggcgccgggc cccgaggtcg caaggcatac cccgacgcgg cccgtagccc tcgcgtatcc
     2341 gtcggcggcg tgggccgccg cctgctcatg cctgacgaga atgtgcctga tatccgaatc
     2401 gtaaatggcg tcatagaggg gcanntcatc ttacccatct atccgtcctc ctcaagttgc
     2461 atcagcctgt tgatcccttc caagacggcc tccacggagg ccatgatgat gtccgctccc
     2521 gccccccgag ccgttatggt ccggtcgccc atcgccatcg taacccagac ctcgactagg
     2581 gcgttcgtac ccccggtgac ggcgtcgacg tggtactcct cgagcctcac gtccctcacc
     2641 ccggagatgg cccggcggat ggcgttgatc gccgcgtcca ccggcccgac ccccgtccca
     2701 gcctccagga tctccgaccc gttgactatg atcctgacgc tggcggtagg aatggcatgg
     2761 tttccgctga cgatggtgaa ctcgttcagc ttcaccttcg gctccttctg gatcgatagg
     2821 acgatctcgg cgatcgtctg aaggtcggcg tcggtgaccc gtttcccctt atcccccagc
     2881 tccttgaccc gggagaggat ctcggcgagc tcgccctcgg tggactcgat ccccagctcc
     2941 ctcagggcca gctcgacgga gcttcggccg gcgtgctttc cgaggacgat cctcctcttc
     3001 ctccccaccg cctcggggtg gatcggctcg taggtcgacg tatcagccag gaggccgtgg
     3061 acgtggatcc ccgcctcgtg ggtgaaggcg ttctccccga cgatggcctt gttgggggcg
     3121 accggaattc ccgtaagcct gctgacgagg cgggagaggg agtagagctc ctgacactgg
     3181 atctccgtct ttatgccgta gagggactcc agggtcatca ccacctcttc gaggctggtg
     3241 ttcccgcccc gctctccgat gccgttgacg gtgacgtggg ccatggtggc cccgccccgc
     3301 aaggctgcga cggtattcgc cgtagcgagg ccgaagtcgt cgtgacaatg gacgctcacc
     3361 ggcactggaa gctccgatag ccgcgagaag acctcgaagg ccttctccgc gactaaaacc
     3421 cccacggtgt cgcagtagca gagccgatcg gctccggcgt cgatccccgc ccgatagagg
     3481 gcagttagat acccctcgtc ggcgcggctt gcgtcctcgc cggagagctc tacgacgaga
     3541 ccgtggccct tggcgtactc ggtcacctcc accgccgccc tcataacccc ctcccggtcg
     3601 gaccggagct tgcaccggat gtgaaggtcc gagaccggca ccaccaggtg gatggagtcg
     3661 acatcacaat tgagggctag gtccacgtcg ccggtccgcg cccggacgta gctgcagacc
     3721 tcggcccgga gcccctcctc ggccaccgct ttcatggccc tcctctcgcc ttcggaggtg
     3781 acggcggagc ccgcctcgat gacgtcgagg ccgagggcgt ccagttgccg ggcgatgctc
     3841 accttctcct ccagggtcag ggagaccccg ggggtctgtt caccgtcccg aagcgtcgtg
     3901 tccaggagac gaactctacc aaataaagcg atcccacgca ttcatattcc atcaccaaac
     3961 tgagaccaca aaaatatggt cgatctccca ccaaggggtt ttttatagtt gataaagctg
     4021 acgacggaag accgccgacg tcggccggga tcttcctgta accgtacgcc gatggaagga
     4081 aagatttatc actcccgggg ggccattaat cgccttcagc cataggacag ctcgatagat
     4141 aggggttcaa aggtcggggt cgggtgcaaa aatggggtca agcgcaaaga cgggatcaga
     4201 ggcggagctg aggggtgcgg agacgtcggg ggagctggag gtcgcggagc tcctcctcac
     4261 ggcggagata tacaaccgat ccgagaacct gaccgaggac gacctcccgc cgaagatcag
     4321 gaaacactac ttcgatcccg tgacgagaaa ggtgaagcgg ccgatctacg tcaccgaggg
     4381 ggacgtccag gagatcttcg gcctcggcag cgtccgggaa gcggcgagga gcctgccctt
     4441 cgtcgaccgg gaggggatcc gcgaccagct gaggctcacc gtcttcgacc tgggggcgag
     4501 gtggttcgtc aagcgcgact ccctggagcg gatcaggtcc aaccccaccc tcgcctattt
     4561 ctacgagaac tacgactccc tcgacgtcag ctacgccgag gcgaggaaga gaaaccagcc
     4621 tctactgact gatcgggagt ggatccagtc gaagatccgg gagatggagg cgaaggtcga
     4681 agaggcggac gagatcctga agctcgtcag gatcaaggcc cccgaagacg tccgggaccg
     4741 gatcgaggac ctggtattaa cgaaggacca ggtgacggag atcgagaagg cggccaaggc
     4801 cctggagcac cgcgattacc tgagagaggt ggggctctac gacatcggaa agctcctctt
     4861 cgtcgggcct ccggggacgg ggaagacctc caccgccatg gccctctcag ggaagttcgg
     4921 cctccccctg gtggaggtcc gcctctcgat ggtgacgagc cagtacctcg gggagaccag
     4981 caagaacatc gacaaggtct tcgacctcgc gaagaccctc agcccctgta tcctcttcat
     5041 cgacgagttc gacttcgtag ccaagaccag gacctccgat gagcacggcg ctatcaagag
     5101 ggctgtcaac accctcctca aggcgatcga cgacgtcagc ctggtggagg acggggtcct
     5161 cctcatcgcc gccacaaacc acccccagct cctcgactac gccgcatgga ggaggtttga
     5221 caaggtggtg agcttccccc tccccgacga ggagatgagg cggaggatct tcgggaaggt
     5281 cctggggagg atggacgccc gggtcgaccc cgaggtcctg gccgagagga cggaggggta
     5341 ctccggctcc gacatccggc tggtggtgag ggaggcggtc ctcaacgccc tcctcgcgga
     5401 caggaggtcc atcgaccagg aggatatgct ccgggcgatc accgagttcg atcgtcggat
     5461 caccgaacac cgggggagcg ccgccccatg agagtgaccc tcctggggac cggagacgcc
     5521 ataggcaccc cgaagatagg gtgccggtgc ccggcctgca ccgacgccct ctgcggcggt
     5581 aaaagcagaa ggctcaggtt ctcgatcctg gtggagaacg gccgcggcaa ggtcctgatc
     5641 gacacgagcc ccgacctcag gtggcagctg atccggaccg ggatcagccg ggtggacggg
     5701 gtgatctgga cccacgccca ctacgaccac tacgccggat tcggcgactt ccaccgggtc
     5761 cagaacaggg ttcccgccta cggcctgaag gagacgatgg actacatttt gaactacctg
     5821 tacttcatga ggccgaggcg gaacgtcgtc gagatggggg tccccttcga ggtcgccggg
     5881 atggagttca ccctcttcga ggtgaaccac ccccccatct gcgaggcggc gggggtcgtc
     5941 gtccgggagg ggaataagaa gctggtggtg acgggagata cgactctcca gatccccgag
     6001 gcgagcctgg acctgatgag gaacgccgac ctcctggtcg ccgacgccat aacccccccg
     6061 ggatacgccc tcaccaagca catgaactcg gaggaggcgg tgggcctggg ccgggagctc
     6121 gcggcgaaag aggtggttct gacccacatc agtcacctct tcccccccca cgagatcgcc
     6181 gccaagaggt ggcccctcgg ctacgacatg atggcgatag agctctgaat cgaaagttgt
     6241 ttatcttttg gatccccgcg atgctatcat ggggggtatc tccgcttctg gaagcctatc
     6301 ggccctattt ggcgccctcc tcctcctcct ctcggtccca tcgggggggg aggggttcgt
     6361 ctgggatcgt gactttgggg ggctcctgga gatccccccc atccggaata gcggggtcgc
     6421 ggttgggccg gagcccgccc cggccggaaa cctatccgat ggggccggtg gagggagggt
     6481 gcccacctca gagctttcgg cggccatcga agccctcttt accgcctcag ggcgcccgga
     6541 gggcggagcc gacggccgga ccgaagctcc cggcttctcc ttcgtcctcc cccccggatg
     6601 gacggcgacc ccccgggggg accaggtctg cggaaacctc accctggagg ggggctgctc
     6661 cctcgcgacg gtcggctggt tcgaggatcc ggggatcgac cccgaggccc ttctccggca
     6721 ggtggggagg gcctatagga gcgaagccct tcggttctcc gtcctcgagg tggagccggg
     6781 taggtcaatc gtcgtcgacg gccagagggc ctcggccctg aacgtcttct acatgtacgg
//
LOCUS       DASM01000145            4681 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_146700, whole genome shotgun sequence.
ACCESSION   DASM01000145 DASM01000000
VERSION     DASM01000145.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_146700"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          760 a         1542 c         1538 g          839 t
ORIGIN      
        1 ccgcctccga gaggacggcg gggttcacga ggattatgta catcgccgcg aggaaggcgg
       61 tggcccccgc cagagcctcg gtcttgatct cggtcccgtt gttgtagagg tcgaagatcc
      121 tggagatcct cgtcgtcaag tccagttcca tccctcccat ccatctttgc cgatccgatc
      181 cgcgccgatc cgatccgctt cttctagttc tcttccgtcc ccgaagttaa gacggttttg
      241 acccgggccg atccggagga aaaggataaa gaccggccct gaccttcgtc ctcgaagaag
      301 agcggatcaa accggtgaac tcccatgaat ctcgaagagc tgatcgctgc ggcccgcggc
      361 gaggtggagg tcgacctcct cctggagggg gcggatctgg tgaacaccct ctccggcgag
      421 gtccatcggg ccgacgtggc gatcttccga ggtatggtgg tgggtttcga ctcccccagc
      481 gcccgcgaga cgatcgacct atccgggagg gtcctggcgc cggggttcat cgacggccac
      541 gtccacctcg agtcggcgat ggtgaccgtc cccgagtacg cgagggccgt cgtcccccgg
      601 gggacgaccg ccatcgtcgc cgacccccat gagatcgcga acgtcctggg ggccgagggg
      661 atcaggtaca tcatcgcctc ggggaggtcg tcgcccctat cggtctacgt aatgctcccc
      721 tcctgcgtcc ccgcaaccca cctcgaaacc tcaggctcca gcctcgacgc cggggccctc
      781 tcggagctga tcgacgaggc gggggtttta gggatcgggg aggtgatgaa ctacccaggg
      841 gtcgtcttcc gagatcctca ggtgatggcg aagctcggcc tcgccttaag ggcgaggttt
      901 gacggccaca aacccaggat cgacggccac tcccccctcc tctctgggag ggacctggcc
      961 gcctacgtcg ccgccgggat cgggtcggac cacgagtgca cctcttccga ggaggcgaaa
     1021 gagaagctcc ggctcgggat gaggatcatg atccgggagg ggacggcggc gaagaacctc
     1081 gacgacctcc tccccctggt gacggaggcg aacgccagaa ggttcctctt tgtctccgac
     1141 gaccgccacc cctccgacat cctccgggag gggcatatcg actccatggt gaggagggcg
     1201 gtcggggcag gcctcgaccc cgtcgtcgcg atctcgatcg gaagcctcaa cgccgccgag
     1261 tacttcggcc tctcggaccg gggcgccgtc gccccgggac ggagggctga catcgtcgtc
     1321 ctcgacgacc tggatgggtt gaacgtccag aaggtcctga agggaggggc cgtcgtcgca
     1381 gatggcggcc gcatgatcgt acccctcccg agggcgccgt cgctccgatc gtcatcgatg
     1441 aaggtcgcgg ggctgggtcc cggatccttt gatataaagg ccgaggggga cctggtgagg
     1501 gcgatcgggg tgatcccgga ccagatcatc acgaggtcgc ttctggccag gccgaaggtt
     1561 gaggggggaa aggtggtctg tgacctcgac tccgacctcc tcgagatggc cgtcgtcgag
     1621 aggcaccggg ggacggggaa cgtcggcctc ggcctcgtct ccggattcgg cctattgagg
     1681 ggcgccatcg ccacctccgt cgcccacgac tcccacaaca tcgccgccgt gggggtgagc
     1741 tcggaggaga tcttcgctgc cgtctcggcg gtggcaaaga tggggggcgg cctggtcgtg
     1801 gtggacggag gaatcgtcgc cgcgtcccta tcgctcccca tagccggcct cctctcggac
     1861 cgatcgatgg aggaggtggc agagaagatc gaggaggtga cgggtgccgc taagagcctc
     1921 ggctccgccc tggacgaccc cttcatgacc ctctccttcc tatgcctgcc cgtcattccg
     1981 gagctgaagc tgaccgaccg ggggctcgtc gacgtcacca ggtttgattt cgcccccctc
     2041 ttcgtcggcg atggcgagga ggcctgaggg gtcggatctc gtgccgggga gttctttgct
     2101 ggatcgggct ggcggcggag ttccggggct gggaagatga gccccaagaa gagggccctc
     2161 gactccagcc ccgctggaat ctggtttcgc tggaacaaga agaacagcta cagcatcgcc
     2221 gccctcctcc ccctggtgaa aggagcgggc ctcgccgacc gcccccggga cgggatcatg
     2281 ctctacagct tcgccaccgc ccaggccgag gaggtctatc gggaggtcgc cggctcaaag
     2341 gtcaagacgg tctatgtcgc cggagggccc cacccctcgg cgaggccgga ggaggcgctc
     2401 caccacttcg actacgtcgt catcggggag ggggaggaga cgctgccgga gctgatcggg
     2461 gtcatcaggg gcgggggcga ccccgagggc gtccgcggca tcgcctatcg gcgggagggg
     2521 gcggtcagga tgacgcctcc gcgtgagcat gtcgacctcg acctctaccc accttttaag
     2581 ccgccgatct tctcccccct ggagatcagc cgcggatgcc cctgggggtg tgcctactgt
     2641 cagaccccct gcctcttcgg gcggaggatg cgccaccggt cgatcccagt gattatgagg
     2701 tacgcccggt gctactcgga cctccggttc acctcgccca acgccctcgc ctacggctcc
     2761 gacggagtcc acccccggat cgataaggta gagcggctcc ttgaggccct gtcggatctg
     2821 ggaaagccga tctacttcgg gaccttcccc tccgaggtga ggccagactt cgtgacggag
     2881 gaggcggtag agcttttggc gaggcactgc accaaccgga ccatcagcat cgggggccag
     2941 tccgggagcg atcggatctt gaagatggtg ggaaggggcc acgacgtcga ggcggtgagg
     3001 ggggcctgcg acctctccct cgccgccggc ctggtcccga acgtcgacct gatcgtcggc
     3061 ctccccgggg agacggcgga agatcaggct ctgaccctag acctcgcccg ggaggtggtc
     3121 gggggaggcg gccgggtccg ggcccaccac ttcaccccgc tgccgggaac ccctctggag
     3181 gggagagagc ccgcccccct ggcagcggag gtggagaagg cgatggggga gctcgccctc
     3241 cggggagttg ctaccggccg gtgggatgcg gccggtcaga ggtagaagcg gcggaagggg
     3301 gtcctgtagt cgtcgaggag ctcgtacttc accacgatcc ggtcgttctc gacgtggatc
     3361 tccctcaccg cctgggagaa gaggtaccag gcgtaccccc ggtccgggat cttcagcctc
     3421 tccgggaggc ttgcggagaa ctccgtctgg ttcaggatgt aagatcggag gagctcgctc
     3481 cccgaggcga agaggaaggg gaagccgacc ctctccacct cctccgccat gcaggggtcg
     3541 gtcagcctca gcctctgctt cagatgctgc cggtactcct cctccgtgat gacaagctct
     3601 ttcagttcgc tcaacgtctt ttcacccccc aaccctgaag cgagatgaga gataaaaatt
     3661 gcggggtcgg gggaggccca ggccgatgag gaaaaaaggg agatcccttc ccgggcgggc
     3721 ggcggtcgtt ggccaaaaag gagggagggc ctgggccgcc acgcctttcg ggggatgggc
     3781 ccggggtacg atccggaagg ttcgcgctcc gcccaccgtt catttatccg gcgccatctc
     3841 accggagccg ggagctcctt cgacggagac ctccaggtcc agccccgaga gggcgccgac
     3901 gacgtcctgg gccatcgtcg ccctcgtctc cgggtcgagg tcctgggata ggacgaccct
     3961 gaccctcctt ccctccacct cgatcccgtc gaccctcttc gccgctacga tgttcagccc
     4021 cttcgcgggg tcgaggacgc cggtcatcat cctccggacg aaatcctcgt ctatcgacct
     4081 cttgcccttt atcgcctcgt acttcatgat cgccgccttc aggccggatg tcgcaagctg
     4141 ggcgcagtgg accttctgct tggggagccc ccccagctcc tccaccaagt ccttgaagtg
     4201 gatccccctc gcctcctcca gggtcttgcc cttggccaat tccgtaatca tgctggagac
     4261 ggcgatggcg gcgccgcagc caaaggtctt gaactnncca gggtcttgcc cttggccaat
     4321 tcggtggtgg cgctggtgct ggcgatgttc gcggcgcagc cgaagctctc gaacttgata
     4381 tcctcgatca cctccgtatc ggggttgacc ttcatgtaga gctggaccat gtcgccgcag
     4441 gcggcgctcc ccaccctcgc cgtcacgtcc gcgtcctcaa tcctcccggg gttcctcggg
     4501 tttttgaagt ggtccaggac cttctcggta tatccaaggt tcgtcattta tcaggcctcc
     4561 accttctgct cctgccttcc gcccgaaatg ccggcggcgt cggcgggaca ggccgctatc
     4621 ttctcgacga gctttgcgag gacctccttc ctcatgccgc cgacgaactt gatcgacccg
     4681 a
//
LOCUS       DASM01000146            5249 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_145065, whole genome shotgun sequence.
ACCESSION   DASM01000146 DASM01000000
VERSION     DASM01000146.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_145065"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    4833..4893
                     /estimated_length=61
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          983 a         1530 c         1807 g          866 t
ORIGIN      
        1 gcccgatccc cggaaacccc tcgatcacga cggggctctc ggatcggggc gcttcagccc
       61 tgacctggac ttcgtcagtc atgaaaatat ctttgtccac tgatgggtga taagagcatc
      121 ggtcatcgcc cttcaagagg aggagacttg ctgtaacaac ttattaattc catcaccgcc
      181 taagatgaag agcaaatctc ggagatggtg tgattctatg ggattcgaac gcgtcgaggg
      241 agaggaccgg ggcgatataa ccctctacgc cctcagcacc tgcgtctggt gccacaagac
      301 caagaagctc ctggagaggc tgggagcgaa gttcagatat gtctacgtcg accttctgga
      361 gggggatgaa agggatagaa ccctggaaga tgtaaagaag cacaatccaa gtgtcacctt
      421 ccccaccacg gtcataggag gcgataagtc catcgtgggg ttcaaagaga gggagatgaa
      481 ggaggccctg ggagtatgac cacctttgag ccctctcgag aggagggtga gaacctccgc
      541 gagcagctgg accgggaggc ggaggagatg ggctatcacc tcaacccaga cctcgacttt
      601 acgacgagcc tcgtccgggg gctcctggtg aacgagagga gatacggcta cccctcatgc
      661 ccctgcaggc tcgcctccgg cgacagggac gaagacctgg acataatatg cccctgcgac
      721 tatcgggacc aggatctgaa cgagtacggt gcctgctact gcgccctata cgtctccgag
      781 gaggtcctgg ccggagagcg ggatctctcg gccgtccccg aaaggaggcc atctcccgag
      841 gagagggcga gggcggcgaa ggcgttatcc gccccgtcgg gggaggcctt ctccgccctc
      901 ccttacccgg tctggaggtg cagggtctgc ggatacctct gcgccagaga cgagcctccg
      961 ggggtctgcc ccatctgcaa ggccaaggtc gatcggttcg agcggttcct ctgatggcag
     1021 gtccgaagac agcgtggggt gagggacgat ggacgagagg tcaaagggga agaagcgggc
     1081 agagctccag atatccggga tgacctgtgc atcctgcgcc gccgccgtcg aggaggccct
     1141 gatggaggtg gagggtgtct ccgaggcgag ggtgaacctg gggaaggaga ccgccatggt
     1201 ggagctcgac ccttccaagg ccgggctggc ggacctggag agggcggtat cggatgcagg
     1261 ctacgccgtc gtgaacgaga tggtctccct gaaggtgggg gggatgatct gcgcctcctg
     1321 cgtctcctcg gtggaggacg cgatcgggga ggtggagggg gtggtcgagg tcgccgtcaa
     1381 cctgggggac gagagggcca gggtgaccta caaccccagg atcgcctccg tcgccgatat
     1441 gaggactgca atcgaggagg cgggatacca gtacctcggg atcgccgggg aggagagcga
     1501 ggggagggag agggaggcgc gggagaggga cctccgggat aagaagcgaa gggcggctct
     1561 gggctttgcc gtcggctcct ttctgatgat catcgatatg gcccacctcc gccttccagt
     1621 cccgatgccg atcttcatgc tggtcatatc cacccccgtc ttcatctacc tcagccggcc
     1681 gatcttctcg gcgggtctcc gggccctccg ccatcggtct ctgacgatgg acgtcatgta
     1741 ctccatgggg attggtgtcg ccttcatatc gagcgtgatg gggaccttcg ggatcctcct
     1801 gacggaccag ttcatcttct acgagaccgc cgtcttcctc gccgccttcc tcaccctggg
     1861 gaggtacctg gaggcgaggg cgaaggggag gacctccgag gcgatcaaga agctgatggg
     1921 gctgcagccc aagaccgcca ccgtcctccg ggacggccag gaggtggagg tggcagccgc
     1981 cgacctcctc gtcggcgacg tgatcctcgt ccggccgggg gagaaggtcc ccgccgacgg
     2041 cgtcgtgctg gagggcgaga gctacgtcga cgaggcgatg atcaccggcg agccgatccc
     2101 ggtcaagaag agtagggggg acgccgtcgt cggcgggacc ctcaacaaga acggcgcgat
     2161 aaagttcgag gcctctaagg tcggaaggga gacggctttg gctcaaataa tcgccctggt
     2221 ggaggaggcc caggggtcta ggccctccat ccagaggatg gcggataggg ccgtcgccct
     2281 cttcatcccc gcggtcctga cggtggcggc cgccaccttc ttcctatggc ggttcgtcct
     2341 cgcagacctc gtccccggcg accccctcct cttcgccctc actgctacca tatcggtcct
     2401 ggtcgtcgcc tgcccctgcg ccctcggcct cgccagcccc accgccatca ccgtcgggat
     2461 cggccgggcg gcggagctgg gggtcctggt caagagcggc gaggctctgg aggcctccga
     2521 gaagctgaac accgtcgtct tcgacaagac ggggaccctc accgtgggga ggccggaggt
     2581 gacggatata ctccccttcg gggtcgacga gagggagctc ctccggatcg ccggagggat
     2641 cgaggccaat tcggagcatc ccctggcgga ggcggtggtg agaaaggcga gaagcgaggg
     2701 gatcgacctc gccgaggcga cctcttttga ggccctcggc gggaagggag tcctggcaga
     2761 gatcgagggg agggacgcgg ccgtcggaaa caggatcctc ctccgggagt tggggatagc
     2821 ggtccccgag gggacggagg cggccgccct ccggatcgag gaggagggga agaccgccct
     2881 cctcgtcgcc tccgcaggcc gggtcattgg cgtcgtcggg gtggccgacg ccctcaagaa
     2941 gaactcggta gaagcggtcg gtcggctgaa ggctatgggc tttgaagtgc tgatgatcac
     3001 cggcgacaac ccctccaccg ccgggattgt agcaaaagag acggggatcg agagggtcct
     3061 cgccgaggtc ctcccctcgg agaaggcccg ggagatgaag aggctccagg aggaggggcg
     3121 ggtcgtcgcc ttcgtcgggg acgggatcaa cgacgcgccg gccctcgccc aggccgacgt
     3181 ggggatcgcc atcgggagcg ggaccgacgt ggcgatcgag agcggcgaga tcgtcctgat
     3241 ccgagacgac ctcctggacg tggtggcggc gatcgagctc agccggaagg tgatcgggag
     3301 ggtgaagctg aacctcttct nncggtggcg gcgggggtgc tgtaccccac cttcgggatc
     3361 gccttccggc cggagtttgc ggccctggcc atggccgcga gctctgtgac ggtggtgagc
     3421 ctctcgctgc tgctgaaggg gtacgtcccc ccctccaaga gggtagctcg agggtgatta
     3481 tttcaacgcc gagatagttc taggtcgctg agaggtgaag attttggcta tagatccggt
     3541 atgcaagatg accgtcgacg agaagacggc taagttcaag accgagtata aagggacagc
     3601 ctactacttc tgcgccccag ggtgcaagaa ggccttcgag aaggaccccg agaggtacct
     3661 gaagagctga tcggcgatat tctaggtcct aaagagccgc aaaagattgg gggtcagagg
     3721 atgaagaagt acgacgtcat agtcgtcggc tccggcgccg ggatgatcat cgtctcccgg
     3781 gggatccggg ccgggatgag ggtcgccctc gtcgaccacg gccccatggg ggggacctgc
     3841 ctcaacaacg gctgcatccc ctcgaaggtt ctgatctacc ccgccgacgt ggtccggacc
     3901 ctggaggcgg cccgggccgt cggggtggag gcccgggtcg agaggatcga cttcgacctc
     3961 atcatgaggc ggatgcgcgc cgtcgtcgat gagggtagac gcggcatgga gaggtccgcc
     4021 gagagggccg agggcctcga ctggtaccgg ggatgggggg agttcgtcgg cgactttctg
     4081 atcaagatcg gcgaccagca gatcacctcc cctcggatct tcatcgcctc ggggtcgagg
     4141 ccgctcgtcc cccccatccc cggcctcgcg gaggcggggt acctagacaa cgtctccgtc
     4201 ctctcgctac agcgccctcc agagagcctc atcatcctcg gcgggggata cctcgcctcc
     4261 gagtacgccc acttcttctc tgccctgggt acgaaggtca ccatcctcgg gagaaaccgg
     4321 atgcttctca aggacgagga ccccgagatc tgcgagatcg taaagaggcg cctctccaga
     4381 tacgtcgacg tcaggaccgg acacgaggcg gtgaaggtgg aggtgaaagg aggcaaaaag
     4441 gtcgtatccg ccctggacct cgccgccgac gccgtcagag agttcgccgc cgaggaggtg
     4501 atggtcgccc tggggcggag gagcaactcc gacatcctca agccggagaa gacgggggtc
     4561 gaggtcgacc gctccggctg gatcgtggtg aacgaccgga tggagacgag ccgggagggg
     4621 atctgggcct tcggcgacgc cataggaaag catatgttca ggcatacagc caaccgcgag
     4681 gcccgggtag cctgggacaa cgccttcggc tccaccagcc gggagatgga ctatcacgcc
     4741 gtccctcacg ccgtcttcac ctgcccccag gtggggtcgg tggggatgac ggaggcggag
     4801 gcgaaggccg ccggatataa ggtcctggtg ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnccatcgc cgggaccggg gccgccgagc
     4921 tcgtccagca ggtcgtttac ctgatgaacg ccggcgaccg cgacagcggg ccgatcgagc
     4981 gatccctggt gatccatcca gccctgagcg aggtcgtcgc ccgggccttc gccaacctgt
     5041 tgccgccgaa ggaagatttt cctcgacgat ctggaggggg ttctgaccaa gatcggaggg
     5101 ctctcgatgc ctctgcgagg gatgaactat gatgagaaaa gccctgatcg gatcggccct
     5161 cctcttgacg gtgggattgg tggtcctcct ctacgccggc ggtatccacc aaccaaaaaa
     5221 gtacctcgac ccctgggaga ggtcgtatc
//
LOCUS       DASM01000147           11141 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_43907, whole genome shotgun sequence.
ACCESSION   DASM01000147 DASM01000000
VERSION     DASM01000147.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_43907"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2118 a         3564 c         3371 g         2088 t
ORIGIN      
        1 agctcgaaga ggaccttctc cccccggggc gagacgacgt agatctcgac ccggttgcag
       61 gtcttgagga cggcggactc ctccaccagg tcggaggagg agatccactt gaggagggcc
      121 tccaggtcgc cgtgccaggc cctctcaatc tcgtcgatat tggccttccg gtgggtcacc
      181 agcatcgagg ctatctcgct catcgagagg gagatgttct ccggccctct tatagccctt
      241 ttcgtaggac tccgatagga gcctccagac ctcgccatcg gagatgatct cccagaggat
      301 ccgcctcctc tcctcctggt cggcgaccct ctccttcagg acccccctca tttcccccag
      361 gaggcgggcc atatcgccgt acccttcgtc caggatctct tcgatcctca tccgcaggct
      421 cttggataga gccgggctct ccccaaaggt ggatatggcg acgacgacgg ggcccctcct
      481 gacgatggag gggacgacga cctccccgaa gccgtcgacc cggttcacca gcacccccat
      541 cgaggatgcc gtctcctcca gcctggcatt gaggaaggag tcgctggtgg cggggatcgc
      601 gatgaaagcg ccccggagga ggtcggcccc gcccctccga aggtccgcct ccaccagctc
      661 cacctccccc gccgccgcca tctcccagat cccgggggtg aactccaggc tcgccaccgt
      721 caccctggca tgccctgaga agagccgggc cttcctttcg ccgacggcac cgcccccgaa
      781 gatgacgacc tttcggcccg agaggtccag gaggaggggg aggtggccct tcagatccgc
      841 gcccccgtct tcttcaactc tctctcgcta aagaggacct gatactcggt gatcccgacc
      901 ttcctggata tctcggcggc gaccttccgg cactcctccc gggagggaga gtggacgacg
      961 gtgtagaggt tgtagggcca ctcgggcctt tcggcccgct cgtagcagtg ggtgacctcg
     1021 tcgaaggagg ccataatacc cccgacccgc tcggcatcct ccggcgggac ccgccagacg
     1081 atcatggcgt tggccacgat ccccaacgcc cgatggccta tcgtcgccga gaacctcctg
     1141 atcacctcca gctccaggag gcggctcagc ctctccagca cctccccctc gccgatggcg
     1201 agctcctttg ccagggcctc gtaggggcgg gaggagaggg ctatcccccc ctgagccgcc
     1261 ctgatgaggg cgaggtccag atcgtcgacc tcatccggcg tcccgtcatc tccttctttc
     1321 atccgaggac cttcgtatga tttcacttct tcctccagcc gtcggggtcc tggaaggggt
     1381 accggtccac ccccctcgtg gggacggtct tgggatcgga ccccggattt ccggtgacct
     1441 gatggtcgct ccagaaattt cctcccacct cggccccccc atcccaggag ttgtcgccgt
     1501 tgtcgacggc gttctggggg ctcattgggt tgccgttatc gatgaagttg ttgtggcgga
     1561 tgatacaacc cgtgcagtcg accagccccg tccccccgag gccgttctcg acgaaggtgt
     1621 tcccatagac cttcccgtcc cgggagtagc tcatcctcag gccgtagcgg cggttattgc
     1681 tgaggttgtt cccctcgatc ttagcgccga cgaccgcctg gaggaatacc ccgtccccgt
     1741 tatcgaaggc tgaggatccc gccaccgtcg ccgcggagag ctggaggagg gccaggcccg
     1801 catcccgatt ctccgagagg tggcttcgga ccaccatcgt cccgtcggag ccggaggctc
     1861 tgatccccca ccggtttccc gaggcgatgt tgttgttcag gacggacctt ggagatctcg
     1921 ataccttgat cccctcctcg gtgttcccca tcgccctggc ctggaggatc gtggcggagg
     1981 tggagttgac gacggagatc cccaccagcc cctccgaggc ctccaccccc ctcaggaccg
     2041 aagcctccga cccctggacc agaaatccgg cctccgagga cctcagggcc gaagaccctt
     2101 cgatcgaggc gcccttcccc ccctggacca ggaacccgat ggtgcagtta tcggctctgc
     2161 tcgcctcaac ctcacagccg gccgtccaga gcatccggac cccggcatcg ttccccagga
     2221 gggtgaggtt tcgggctgtg cagccgctcg agttgacgat gagggctcca gcactgttgt
     2281 tggagagggc caggctctcg acggtgacgt tggtgcagcc caccagggcc aggaggccgt
     2341 agtctccgga gatggaccgg tcatgctccc ccaccagata gcagaaggcc tttccgtcga
     2401 tggtgttgga ggagtcggcg tcctggcgga agggagcgtc gtcggtggcc gaggggtctt
     2461 ctcgatcgac cctgaaggcc cggtggttat attccatccg gtttttccgg aggaggttcg
     2521 ccccggatcc gaggagggtg aggccgcggc cgttcttcag gatctggttc tccaagatgg
     2581 agttcccgga ggagcccgac ctgaagacca gcccttcgac gttcccggat aggaggtttc
     2641 cggcgacgac gttccccttc gagccctcca gccagatccc ggcgacggcg ctctcctcca
     2701 tccggttctt caagatcctg ttcgccgtcg agtccttcag cctgatcccg acgttgcaac
     2761 ccagggccga gttttcggca actgtgctgt taccggaggc gaggaggagg atcgcgacat
     2821 tggtcgcccc cgtcacgacg ttctccagga tctcgctctc ctccgacctc tcgacggcga
     2881 tccccctctc cggcgaggag atctcgtttc ctctgacccc ggttctgggg gagtcggtga
     2941 tgaggactcc ggtcctggcg gtgatgaggg actcggagac agcacacccg ctacagttgg
     3001 cgatcacgac cccggtgcct ccggagatgg tgcagttgga gatggcaggc ccgagggcct
     3061 cgacccccga gactgagctc tcctgcaagt cccggacggt gccccccgag aggaggaccc
     3121 ctacggagca gccctcgatc gccgagccga cgacggcgtt atcggagtcg gacgaaagga
     3181 tgccggtcga gaagttccgg attatgcagc cccggatggt ggcctctttg gatctcagct
     3241 ccaccccgac gccggtccca gagcccctca tctcgaagcc cgagatgttg gtgcccggag
     3301 cctccaccct gaaacccccc tcgagggtcg tctcgccggc ggagccgttg agggccaggg
     3361 gccggaggag ggggatcacc ccctcccgat agtacccggg gcggaccagg atctcgtcgc
     3421 cgtcttcagc ggcgttgacg gccttgctta tcgtcgaaaa cccttcgcct ccgctcggat
     3481 cgacgactag agttgcggcc tgggccccgg gctgccatgg gctgaatata aggagaagga
     3541 ctatgagcca taacctatcc gtgacgacac ccccaatccg gtacgatagt ccagtggcgg
     3601 cggtgataaa taactctatc cccaggccgc taaaggcgga aaaatccttc agggatcgat
     3661 gagtacctgc ctcctgaggt ggagaggacc ccgcagcgga gctcaaatcc ggccgccaac
     3721 gggaagattc taatcggatg aaattgaata tttgcgggat gatcccggaa ggcgggccgg
     3781 catgaccagc ccttatcgga tcctcatcat cgaggacaac cccgacgacg tcgagctgat
     3841 cctcctcggc ttctcgaagc acgacgagtt cttcgtagac gtcgccacca ccggggagga
     3901 ggggctcgat agggtcgcta cgagccgata cgacctcgta tctgtcgact tcgccctccc
     3961 cggcataagc ggcctggacg tcctggagga gatccgaacg tccgaccagg acgtccccgt
     4021 cgtcatggtg acgggccggg ggaccgagga gcttcaggtc gtcgccttcg agaagttcgc
     4081 cacgagctac gtcatgaaga gcgtcgacag cttccggagc ctccccaccg tcttcgaggc
     4141 gctgatcatc gaggcgaggt tccggtccca ggagcggcgg atgaaaaggg agatcgagag
     4201 gtccgagacg gtgagcaggt acatcctgga gaactctccg acggggatat acgtcctcca
     4261 gaacggctgc ttcaagatgg tcaactccag gttcgctgag atcttcggct gcgataagga
     4321 ggatcttata ggaaagcact tctggagcct ggcagacccg gagagcttcg agtgcaaaga
     4381 ggccgccgag gagccctgcc tggaggtcgg ggccgatctc ccaaggacga aggtccacga
     4441 gttcggcgtc accaggaggg acgggtcgaa gcggtgggtc gaggcgagga tcgcctccct
     4501 cgacgaccag gaggagaggt gggtcctcgg aaatgtggtg gacgccaccg atcggaagag
     4561 aggagagagg gacctcctcc tggcgaaccg gaggctctcc atcctccaca acctcctcct
     4621 cagggcgatc gactttcccg gtgactccga cgacctgatg aggtatgccc tggcagaggt
     4681 gatggccggc ccggacggat tcgaggtggg gggagccttc ctccaggaag gtggacggct
     4741 cgtcctccgg gcgatggccg gacccctgga ggccctcatg gagctctccg agaaggtgga
     4801 cgccgagggg ctcctagagg aggacaagat gaggacggtg gcgggggacg gccccaccat
     4861 atcctgggcc atagccccct tctcctgcgg cgaaggccgg aggggtctct tggttctggg
     4921 aaggggggag gagatggggg acgaccttca gaagttcgtc gccgaccttg cctcccactt
     4981 ggggaagatg gtgggagggg agcctccgag cggctgaggg ggcgccgaga ggggctgatc
     5041 ggggatgggg ggctacagcc gcctccagat gagggctatc ctctggagag cggtgatgtg
     5101 gcttgccagg gtgatcacca ctacagccca ccccaggata gatagaccca tgagatcccc
     5161 ggggaagagg gcggtggcga aggtggctcc gaggatgatg acgagccggt cggccctgcc
     5221 catgatcccg ccgtagaggc ggccgaggct tagggcctgg gcctgggtcc cgaggtagct
     5281 cgtcaggagg acccccatca cggcgaagat ccccaccgcc tcggagacgt agcccgcgaa
     5341 gacgatcccc gtgaggatcg ccatgtcggc gtacctgtcg atgacgtggt cgaggaagtc
     5401 tcccctcgcc tccgcccggc cggccttccg ggctacggcg ccgtcgacgg cgtcgaagac
     5461 ggcgttgagg aggaggaaga gggctgcagg ataaaggaaa gcgggacctt cggccgagag
     5521 gtagtagaat ccccccgccg ccagggcgaa gatcagggat aagagggata tcaggtcggg
     5581 ggacgccccc acccccatcg ccaggtccgc caggggctcc gtcgcggcct gggacctgga
     5641 cctcaggtca tcgagggtca aactccacct ctccagtcca atctataccc ccgggcggaa
     5701 gagagatctc gcggcgaagg agcctcgcca ccagctccgc cacctcctcc ggcgatagct
     5761 gggtcgcgtc gatctcgctc acccggtcgc acccctccac cgcctccacc aggacgacgt
     5821 ctagggcttc agcctccagg ttctcccgga tcttcctctc ggggtaaccc cttcggccga
     5881 gccgctccct caggaccggg gggcgggtcc ggaggacgat cgcctcgggg gcgaagcggt
     5941 gggagaagtg cccctccagg acgacgatct cctccgaccc cgccggggcc agctcctcga
     6001 tcctcgcctc cagggcttcg acgtcggcga tcagacaccc ccggccctcg tcgatgccga
     6061 cggatagccc ctcctccctc accagggcgt ttatctcgat caccctgaag gggaggaggg
     6121 cggcgacggt cgtcttcccc gtcccagggg tgccggtcag ggcggcccgc atacctctcc
     6181 gaaggcttcg aggaagcagc cgttctcctc gggggttccg accgtcaccc gaagggcgcg
     6241 gtcgcccgcc ccccggaagg agtagcagtc ccggacgatg acgcccctgc cgaggagccg
     6301 ctccgccacc tcccccgagg gctccgccgt ctccatatag aggaagttcc cccccgaggg
     6361 gcgaaactcc gggatcgccg ccatcaaccg gtccctctcc agccttatag cctcaacgga
     6421 ccgcctcatg tgctcgagat cctccagggc cgcgaccccc gccgccaccg acggtgtggt
     6481 gacgccgaag aagggagggg ccgccctcct gtactctctc ccaatccaat cgggggcgac
     6541 ggcgtagccg agcctcatcc cggcgaggcc gaaggccttg gaggtcgttc tccccaccac
     6601 caggttctcg taatcctcca ccagggggag gaagttctgg tcgctgaact cggcgtaggc
     6661 ctcgtcgagg aagactatcc cctcggcggc ctcgacgatc ccccggaggt cctcttcggg
     6721 gatcgtatcg ccggtagggt tgttcggaga gcagatgaag atcatcttaa tcccgctggg
     6781 cacctccgac ggcacctcga actcggggcc ccgggggagg aagaacggat cggccccggc
     6841 ggtcaccgtc aggatctcgt agtagctgaa ggtgggggga tagatcaccg ctctatcgcc
     6901 ggggtcgagg aagagcctcg tcagggtgtc catcaccccg tccatgccgg cgccgatgac
     6961 gacgttctcg ggaggtgctc cggcgtaacc ggctagggcc tcgatgagcc gtccggactc
     7021 ggggtacctc tcgggcctcg cagaggcgat cgcctcatag accttcgggc tggggccgta
     7081 ggggttctcg ttggagccga gcttgacgat cccgtcgggc ggcaggccgt acttcgccgc
     7141 gatttcctgg atgccccggc cggcgacgta gggcttcagc cttgtcaggc aggacctaag
     7201 gttctctttg tatctcgcca aggaccttca ccacgcggtc gatctccccc cttccgacga
     7261 ccaggggcgg gaccatcctg aggacatgat cgctggtggc gttgaggagg acgccccgct
     7321 cccgggcccg gttgacgatc tcggggcagt tcgccgcaag ctccacgccc accatcaggc
     7381 cgaggcctct tacgtcgatg gcgtcgatct cctcggagag cctcgcccgg aggtatctgc
     7441 ccatctcttc agaccgctcc accagccgtt cttccctgat cgccccgatg gaggcgaggg
     7501 ccgccgccga gaccagcggg ccgccgccga aggtggaggc gtggtcgcct ctcttgaaga
     7561 cgtcggagac ggactctgcc gccagcatcg cccccatggg gaggccgccg gcgatcccct
     7621 tggcgagggt catggcgtcc ggcataaccc cgctgtgctc ctttccaaac cacttgcccg
     7681 tacggccgaa gccggtctgg acctcgtcga ggatgagcag cgccccgcag tcgtcgcaga
     7741 tctctcgggc cgccctcagg taaccgggct tggggacgtg aacccccccc tccccctgga
     7801 ccggctccag gatcaccgcc gaggtctccg ttgagaccgc cgcggccagg gcctcggggt
     7861 cgtcgaaggg gacaaagtcc gcctcttgga gggggcggaa gggctccctg tacatcggct
     7921 tataggtcgc tcccagagac cccaaggtcc tcccgtggaa ggagtggatg gcggcgacga
     7981 tcttcgacct ccccgtcgcc ttccgggcga gcttcagggc cgcctcgacg ctctcggccc
     8041 cggagttgca gaagaaggct ctatccatcc ccgtcagggc cttcagctcc tcggccaaaa
     8101 ggacctggtt ctcggtgtag tagaggttcg aggtgtggat cagctctttc gcctgctgcc
     8161 ggatcgcctc gacgaccgaa gggtggcagt ggccgacgtt gttgacggcg atcccggcga
     8221 cgaagtcgat gtactcgcgc cccgaggcgt cccagacccg cgcccccgac ccccggacga
     8281 ggaggatcgg ctggcgggtg taggtctgga ccaccgattt cctctccacc tccgcgatcc
     8341 gggcggtcct cttggcgccc gaagattccg cgttcaagtt caccgtcgcc ttctcctctc
     8401 ccttcttcat accctcatcg gtattcatca tcctcaaatc gccctcttta tatcgccctc
     8461 tttatcgatc atagaacgct ccgagcaaac cggaggctcg cctggatctg gcggccgacg
     8521 tcgccgctga cgacctccat gtggccgggg tagaggacct cgacgtcgag ggccgtcagc
     8581 ctctcgatgg agcggatcag gtccgcgtcg cagccggcga ataggtcggt cctcccgatg
     8641 ttcccctggg ggaagacggt gtccccggag aagaggaccc gagccattgg ctcgtagagg
     8701 cagatccccc ccggcgagtg gcccggggtg tggaggaccg aaagccgcca ttcgcccagg
     8761 tcgaggacgt ccccctcctt cagggtcccg tccgcctcga tctccggggc ggagaggccg
     8821 aagaggtagg ccaccgtggc catgtcgtcc ccgagggagc ccgcctcctc ctcgtggatc
     8881 aggaccctcg cccctgtcgc ctcttttagc cggggcaccc ctgccgagtg gtcgtgatgg
     8941 cagtgggtga ggacgatcat ctcgatctcc tgagggtcga tgtacttttt gatctttcgg
     9001 agcgtcgact ccggctccat ccccgcgtcg acgaggatcg ggacctcatc gaggataagg
     9061 tagacgttcc cgtcgaaggg cgagcctgag accttgtgga cttccaccat tccaacacct
     9121 actccggagg atgggctcca ccgatatatc tgtttggcag cggtattgtg gagcgggacg
     9181 gctcgatggc gatccccgtc cctacagaca cgcgcaacct ccgaaggcga ggcgccgtta
     9241 aagaactccc agaagccgag gcggcggatc accgtctggg aagtttatgt ccggtgtagc
     9301 gaggagatgc tgccggagga ttggctaacg gagatcaagg tcccggtcaa gaggtatgtg
     9361 atggcgcgag ccatcgcggg agagagcgga gttccactgt catcaggaag taagaatata
     9421 atcctctgtg gagactgacc atagcgttca cgccggaatc gataactaaa ttttctgcaa
     9481 aatcacaatt ttctcatcac agttcatctt aaattaatgt aaaaatttta aaaatatgaa
     9541 gggccaaaca gaaacctacc agtagaccct gcccaacata ttttccggcg aattcgccca
     9601 tgctatattc tcgtctgaaa tacgataata tgaagactca ttggatgaag ttgtcgagac
     9661 gtctgttgtt atataaccga ctttcgtcga atcggtgaac tctactgaga ccggtgtctc
     9721 gtttcgcaga acgtctaaaa ccgcctcgaa ttgagaccac tcataagtca gccaaatttt
     9781 tccatcctga tacctattcg agggcaaggc cgttgcgtcc ctgtagaatt cgcaactagc
     9841 tacattcttg ccgtcattaa aacaatgaat ataagccacg agatcgccgc cctcttggcc
     9901 ctttccgtat aaaacaacgg agtaagaatc gatagacgat cttctagaga cctccgggct
     9961 ccatggattt atttcccgcc ccgtatcctc gccactttgg tatggtattt cgatttccgc
    10021 tccactttga gcagaggcga ccagcattat tatggtaaga aaaaacatcg tgcttaagat
    10081 ccaaaatccg ttcttattca tcgtagcgtc actccgttta ctttaaatag gattataatc
    10141 ttcaagatag ttaaatactt ccataatatg gatacgaaac ggacttaatt tagagaaact
    10201 tatatccatg atttgagttg ctgaatttaa aatttttaaa aaatacgact tataggtagg
    10261 gaggccatcc aaaatgtttt tgtagaaatt gtgagtatat tttgctggag tttcatcatg
    10321 acgagatcga ttattttagt cctgtcagta tttgctgccc ttactttgcc tacatgtggc
    10381 cagcagaccg cagaggaatg ggtagaagaa ggcattgctc tcgctctcca gggcaactac
    10441 acagaagcca tcaagacttt cgacgaggcc atccggctag atcctgaata tgctcgcgcc
    10501 tggaatggca aaggtattgc tctctattac caggacaatt acaccgaagc cgtcaaggct
    10561 ttcgacgagg ccatccggct agatccagaa tatgccggcg cctggaacaa taagggcatc
    10621 gctctctatg acctgggcaa ctatacagaa gcaatccggg cgtacgatga ggccatccgg
    10681 ctagatccag aatatgccaa tgcctggaac aacaaaggca acgctctcgg aaacctaggc
    10741 aactacacag aagccatcaa gactttcgac gaggccatcc agctagatcc tgaatatgct
    10801 cgcgcctgga atggcaaagg tattgctctc tattaccagg acaattacac cgaagccgtc
    10861 aaggctttcg acgaggccct ccggctaaat cctgagtttg tcgctgcctt ggataacaaa
    10921 ggcaatgctc tatctaagcg gggaagctac accgaagcaa tccaggctta caacaaagcc
    10981 atccagctag atccagaata tgccggcgcc tggaacaaca aaggcaacgc tctcggaaac
    11041 ctaggcaact acaccgaagc aatccgggcg tacgatgagg ccctccggct agatccagaa
    11101 tacgccaatg cctggaacaa caaaggcgaa gctctcggga a
//
LOCUS       DASM01000148           13458 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_90325, whole genome shotgun sequence.
ACCESSION   DASM01000148 DASM01000000
VERSION     DASM01000148.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13458)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13458)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13458
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_90325"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2349 a         4468 c         4202 g         2439 t
ORIGIN      
        1 gagaagataa gatgaagtct aaagatgtta aatttggttt cgtggccttg ttggccttgt
       61 cgatctttgt tccgacctcg gcaggcttca ctgtcagcgg cgcgcttttc atagatgttg
      121 tatcgccggg tgaggagatc ctccacgaga ttacagtaac gattgatgag gattcatcgc
      181 ctcagaatat gatcgctgtc atcgggggat ttgcaacgac tccggagggc gcagattata
      241 tgcttgatcc cgatgatgat atcggtcctt atactgctag gccgtttttg attgtctccc
      301 ctgagaggtt ccaactccag ccaggcgagc cacagaaggt tcttgtcaca gggaccgtcc
      361 ctgaggatat cggagcgggt ggcagatatg ccctcgttgg cataaagaca gaacctgatc
      421 ccaccggaaa cgtcgcagtc accacagcga tccttgttcc cgtgctgcta cagatagatg
      481 ggtcggagat cgtcgaaacc ggcgagatca cagatctgga ggtcttggag agcgatgagg
      541 gcgtcatagt tgccctcatg ttcgagaaca ctggaaacta ccactataag cccatcgtcg
      601 aagcggttat taaaggcgag gatggggatg acattgctac tgcaacgcct caacagagct
      661 tcagctcagt cagaccgtcc aactcctgga tctccaagat gaatttcgat cttgatgaag
      721 aactagctcc aggcacctat accgtggagg cgagagcgat caaggaggac gggacggttc
      781 tcgacaccga ggagacgacc ttcgaggtct gaggtggcct tgagaggctg cgggtagggg
      841 aggacccaag agaatgggag aatgggaaag ctggaggatg aggggatgag gaggctttgc
      901 gaggagatga ggatcgtggc cgatgagatg aagcgctggg gagggttcct ggccctcgtc
      961 ctcctcgcct ttctcgcggc gccggccctg ggggatgagg cggcggtcgg ggaggggggc
     1021 ggggggtatc tgcccctggg agacggctgg gagaccaaca cgctatcgat cgaggtgcca
     1081 gatatcggaa acttcccact catcccgggc cagaataacg ttaacagcga ggctgtatac
     1141 gccacgataa ctacggaagg cgcgtggacc ttgtcggtct cagatcagga caagaccgtg
     1201 acaaacggga agatgaccga atacattatt gagggcggcg cttacgtcag tcctgatccg
     1261 gataagcttg gggccgccat gttggtcaag gcgacgggaa gctacggctc cgatactaac
     1321 caaacccttc ccaccggagg tcagatagcc caaggtggtt ctgcattaac cgatgagcgg
     1381 attcccatca ccttcaccca gccggtatcc tgggacgatc aggtctcaga gaccggtcgg
     1441 ggttacaaga tcgtggtaac tttcaccata tccccaggct catgagaaga ccaaggccgc
     1501 tctcaatggg gcggccggga taaaatttat ttcgtaatat aaaatttaac ggttcggaga
     1561 atgagagatt taaccggacg ggagaggagg agttccagtc atgaggagga ggagaaaaga
     1621 tcgagcgggg gccctgcccc tggcgctgct gttgatcctg gcggccctat tttgcgggct
     1681 cgcctcggcc gaagaggggg aggccggagg tgacggcgga gccgaaggcg tctactccat
     1741 agcctgcaac ctcccccccg cggtcgtcaa gaacgtctcc ctatccgccg aggtccccaa
     1801 gggtctcgtc tataaatccg agagcctctc catatccggc gccgccgacc tcgctgccgc
     1861 cctcgtcaca tccagcggcc ccgcggacgg ctccggcgcc gtcgccctcg ccatcttctt
     1921 tccccaggtg aacaattccc tcgatctcga tattctggtg gagttcgtcg ccgtcgtcgc
     1981 cgacatcccc gagaactccg acggcgcggt cttggcggac cttcccgtcc gcctctcctt
     2041 cgacgatggt ttggggggcg agagcagctt ctccggctcg attgcggggg tctccgtcgt
     2101 cgagcctgcc ttgggcgtga ataagcgggt cgtgggtccc acggccgacg gcaagggcct
     2161 cgtctacgag gtcgcggtct ttcacacctc ctcttccacc tcgaccgcct acgacgtaat
     2221 cctcacggac ttgctgccgg ccggatggtc ggtctccggc ttcgtcaccg tagccggccc
     2281 cgcctggaag tccgcggtcc tcggcaccgg gccgaccgtc accttcgagg agatcggccc
     2341 ggcctggacc gccgcctctc cggtggtggt gaggtacgag gccgtctcct tcgcctccga
     2401 cggctccgcc tccgacggct ccggctccgg ctccgccggc tccgatccgg ccgcggccgg
     2461 ggggaccctc gcctggacca gcgccgcagg atcggtggcg gaggagcgga gctacctcct
     2521 cgaatacgcc ctggacccct acgcctccct ggagctatcg atcgaggccg acgtctccgg
     2581 ggtcaccgtc ggcgatctcg tcacctacgt ctaccgcctc agaaactccg gcttcgccaa
     2641 cctcaccgat ttagccgtcg tcgacgccgc cctggggccg atcctcctcg acaggacggc
     2701 cctcgccccc ggcgaggccg ccctcgggac cgcggagagg gccgtcgtct ccgccgactt
     2761 ccccggcccc ctggtggcca acgccaccgc caccgggatc gccgaaggcg gcggagaggt
     2821 cgccgccacc gcctccctgg agctctcctt cttcgagaac cacctctcca tagtcaagag
     2881 ggcggacaag aagtcggcgg agcgggggga gctcgtcaac tacaccatca ccatcgccaa
     2941 cggcgccgtc gatagccccg tctctcccac cgacctcgtc gtcgaggacg tcttcagccg
     3001 ccccgtcgag atcgtctcgg cgtcgcccca gcccgactgc gacgggaaat ggcgctaccc
     3061 cgagcttccc gccggccaaa ataggcagat caggatcacc atccgggtcc ccgacgagca
     3121 ggacttcgcc ttcgactcgg cccaggcggt caccggcacc ggcttcgtca gcgtcagaaa
     3181 cgagtactcc accgcctacg agccctaccc cttgcggaac tgcgcctccg tcacctacac
     3241 ccacggggcc gagaagaaca cgagaaagct caccagctgc gcctccgtcg ccatccgggg
     3301 cggggcgggg acgagtctct cgaccaaaga gcacggctcc ggcacctacg cctccgggga
     3361 ggtcgtcgcc ctccggacgg agaaccggtc catctccttg gagaaggacc tctccgcctc
     3421 cgccggcgag accaccctgg ggctctaccg gggcaggacc gtcacctact cctccccctg
     3481 gtcctcctcc gccgtcgggc gaaaccgggt gacgggggcc tcgatgaccg aggcctaccg
     3541 ccgctcctcc tggatagatc gggagtcccg gttcttcctc gacgagaacg agtcggcgat
     3601 ggccatcgat tccgagttcg acgggaccgc caccttcggc ttcgtcagga tgcccgacaa
     3661 ctcctccttc cgggccaccc ccctctacga ggcgagggag gagtactccg gaagcttccg
     3721 ggtcgcagaa aaggtcgacg agtacggctc cggcgtcaaa tccgagcgga ccgcctccgg
     3781 gtccggcatc gtcgccgtag ataaaagggt cggggagacc cagaggagct tcgagcacgg
     3841 ctcggggacc tacgaggcgg acgaattgat cgagacctac accagctaca tcgccaagaa
     3901 gatctacgtc tcctacgagc ccctcggccc cggctcctcc ctctggaaag aggggatcgc
     3961 ctcccagaag cccggaataa gcctcctcag cgaggagtac tcccagatcg cggagctgga
     4021 gaaggactcg atagccaggg gcttgaacga gctggagacg accgccgcct tcagcggcac
     4081 cgcccggtac cgggtcttat actccaacag gacccgggac ctcagcgcca acgcctccgc
     4141 cgccccctgc ggcgcctgcg gagcctgcgc cgatggcgcc gatggcgcca actgcaccgc
     4201 cggcggcgtc gcctcctgcg acgccaaagc ctcccggcac cgccccgaga tcgatatcga
     4261 cgagatctac tccggcgact accgggtcga gcggcgggtg atgatctccg gggcgtcgaa
     4321 gtacgaccac ccccacctca acgtcaccaa ggccctcgag gggatcgaaa ccgaggagct
     4381 ggactgggat tacggtgagg cccacctccc cggggaggtg aagacgagga gggtcgctga
     4441 ttacaccatc cggatcgaga acgacgggag ccgggccctc gcccccatcc tcgtccggga
     4501 cctcttcccg ccgggggcga cctatatcga ctcctccctc cggccgacgg cctccgccgc
     4561 cgggagcgcc aactggaccc tcacccacct cgccatcggc ggggtggccg agatctccct
     4621 ccgcctcgac gtcacccgct tcgccggaga cgagatgacc aaccgggtcg aggtctgcgg
     4681 cgggtacggc tccgactggg tctgcgccgg aaattacacc tccctccaga ggcggtggct
     4741 cgcctgctgc cccgaggacg agatcaccgt cgtcaagacc gccaggatcg accccgcgaa
     4801 ccccctgatc gtcctctaca ccgtcgtcct cgagaacacc gcctctgcga cgagggcggc
     4861 gacgatcacc gactacttcc cccaggggat ggccctcctc gactcctccc accccgtggc
     4921 gaaggtggat gagggggaga tcgtctgggt cctccccacc atggccccgg gagagaaggt
     4981 gacgatcgag ttctccgccc tcgcccccgg ccccggcagg tacaccaacg tcgtcgaggt
     5041 ggaggcgagc tccgtcgacg gccccggggt tgagcctttc cacgtcgcct ccgtcgtcca
     5101 ggtcggctcc cccgaggagt gcggccccgc aggctgcggc atctggcagc ccccggcctg
     5161 ggacttccag cggatcggct acgtccccga ccccctcggc tgcgagatct tcgccgggga
     5221 ggagggcctc ccggaggtat gaggggcccc tcgcccctcc ttcctcctcc tcttctcttc
     5281 ctctcttcct ctctcctccc cttcgacccc tcaggaagat ttttagcccc ccgccgccct
     5341 ctagccggcc atgaccgata ttaagaggat cataccctgc ctcgacgtca aggtcgtcga
     5401 cggccgcccc tccgtcgtca agggggtaaa gttcgtcgac ctcaagaggc agggcgaccc
     5461 cgtcgagctc gccctccgct acgacgccga cggcgccgac gagctcgtct tcctggacat
     5521 caccgcctcc gccgagggga ggaggacgat ggtggaggtg gcccgccgga ccgcccggga
     5581 ggtctccgtc cccttcgccg tcggcggcgg gatcaatagc gtggagggga tcgtggagat
     5641 cctggacgcc ggcgccgaca aggtggggat caacaccgcc gcggtgaagg accccgacct
     5701 gatccggcgg gcggcggcgg ccgtcggctc cgaccggatc accgtcgcca tcgacgccgt
     5761 ccgaaacccc gtcccgaagg agggctcgga gctcttcccc ctggaggacg gaagctccgc
     5821 ctggtttgag gtggtcatcc tcggagggcg gaaggccatc ggcctcgacg ccgtcgcctg
     5881 ggcccgccgg gtcgaggagc tcggcgcggg cgaggtcctc ctcaccagca tggaccggga
     5941 cgggacaaac gacggctacg acatccccct cacccgggcc gtcgcctcgg cggtggggat
     6001 accggtgatc gcaagcggcg gggcgaagaa tcccgagcag atgctccagg ccttccggga
     6061 ggcgggggcg gacgccgccc tcgccgccgg gatcttccac cggggcgagt acaccgtccg
     6121 gggggcgaag gagtacctcc ggtcgaaggg ggtcagtgtc cggctttagg gtgggcgccg
     6181 aaggcgcccc ctgaaggcgg gaaaaatttg agcttgaacg agcccctagc ttctgaccag
     6241 ctctcttagg tccttgtata tctctgagga tatggccaag gccgcccccg cgaagaggag
     6301 ggccccaaag atgacttcca gtatcataac caccgcttcc ctatactcta tatagcagta
     6361 tattctatat atactttgcc tccccctgcc gccttcgccc ccaggaatgg caaaaaattt
     6421 tgtcggccgc cacccttata tcctcaggaa gtattgaatc ttctggaggc ctccatcgat
     6481 gagctttctc atagatccgc cccttctctt cctctcaggg atcttgatct acttcttggg
     6541 ccgccgcctc ggctggaata gacatgcgaa gattgtggta ggggtaggtc tgctgcttgt
     6601 atttataatt tttagctccc tattgtacgc cgacgtattc cgggccgtct tccccctctt
     6661 ccccgaggcg accggatcgg ccttcatgct ccactccagc tggacgaagg tgacgaggga
     6721 gatggtcccc acccccgccg tcgtcctcct cttcctcctc taccccctct ggctcttcgc
     6781 cgggtacgcc ggcgtcctcc tcctgacgaa acggaggtgg gtcaccaaag agctcctctc
     6841 ccgggaggac gtccggagcc gccgcccccg aggcccctcg gtctactccg tggtgaggga
     6901 ccccgacccc cggcgggccg tccgggaggc ggtggccgcc ctgggcggga tggagcagtt
     6961 cgtcggacct ggggaccggg tcctgatcaa ggtgaacatc tgcggcgggg tccccgaggt
     7021 gaaggggacc ttcaccagcc ccgaggtggt cggggaggtc gtcgatatgg tccggggggc
     7081 ggggggcgag gccgtcatat gcgacgccga catgatctgg aacaagttct ggaccgtcgc
     7141 cgccgcctcc ggctgggccg cctgggcgga ggagaagggg gtccccctcg tcaacctctc
     7201 cgagacgagg atcgtcggct tcgacttcgg cgaggggtcg gccatcggcg tcgaccacgt
     7261 ctcccgggat ctggtggagg cggacgtcat catctccatc cccaccatga agacccacct
     7321 cctgacgggg gtgaccctgg ggatgaagaa catgtacggg accttcccgg aggtggacaa
     7381 ggcgcggtac cacaagatga agatcgagga ggtgatctac gaggtgaacc gggccttcac
     7441 cccgaacctg gtcatcgtcg acgggtcgat aggaaacgag gcgatcggcc ccctcagctc
     7501 caggcccatc gactttcaga ccatcatagc ctccaacgac gtcgtctgcg ccgactccat
     7561 cgcctctcag atgatggggt acgacccgtc ggaggtggtc cacctctccc tcgccgccga
     7621 gcgggggctc ggggacgcct ccaagaggtt cgacctcgcc tccctcccct acaggcacgc
     7681 cagcggcaag gacgggagct gggaccggcc cgaggcgaag gtgaaggact tctacaactg
     7741 ggggatcgag ctcttgctga agtttcccgg gtggacgacc ctcttcaacg tcggggccga
     7801 cttcttcctc tacgacatgg cgaggctccc cgtcttcagg tacctcaccc cggggctgct
     7861 gaagctcttg aacgactcgg tctacctcgt cctgcggggg cagggggata cggaggcgga
     7921 cagatcacgg aggaggatca acgtcttcct cctcctcctc ctggcagagg cggcgatcat
     7981 cggcttcttc ctcgacggct acctcatgag ctccttcctc ttcaacctca acttcctcgt
     8041 cgccatcgcc gtcgcgatcc tcgccgctgc gaggatgaag acccgtcacc tcctcgccct
     8101 catcctctcg acggcggcgg tgatgatcgt cgttgagagg atcttgacat cgtcggggat
     8161 cgtagattat aaaggaagcc tgggccccac cctcttcgtc gtatcggggt ggaccctctt
     8221 gatggtggcg atctacggga tatcggatct cttcaggctc tggtttgaga ggctccacct
     8281 cttcgaccgc ctcgataggt ggcggccgct tcccttcgcc gccgccgccg ccgtcttcgc
     8341 caccttcttc tatctggagg ggtacttccc gctggtggga ggggacgttc tggggctcta
     8401 cgccgccctc atcctcctcg gcctactcac ctccctccgg gccaccatcg cctggaacgc
     8461 cgccctggtg gtggtgagcg tggccctggg ggggtatatg gagctcttgg gccacagcgg
     8521 tgggctgtgg tcctacagcc tgacggaggg gctccccatc ttcatgacca tcgccaccgc
     8581 catcaacgcc gctgccgtct atgccgtcgc ctccatcgcc ggggtggacc tctcccgctc
     8641 caccgccggg aaggcggagg acccgtcttc cggccgtgct gggtccggcc ggggacgggc
     8701 tgctctgcca gcgttctaaa gcttccctgc ggccggagcg ggggccccca gatcttccag
     8761 aataataggc gcggaaccgg tcgctcttgc ggtgcggatc ctccggctgc ctcctcggat
     8821 ggggtgatct cctgcgccga tggtctttgc gaagatcccg acgggggcct cggcctctct
     8881 ccatcgcaga gatatgccgg gaggatcttc tcgatgccgg tccgtcggat ccgcccggcc
     8941 gccttccttc tccgagatca tccttccaga atgggctcac gcaacccgtt tgaaatttcg
     9001 taactatatt tgttacaaat agaaactttt atatgaaaaa aggcggcttt agcctgcggt
     9061 gttatatgat gaaatggaat atcgtcatcg ccatggctct cctgagcctt cttttggccc
     9121 ccgccgccgc ccacagcctc tgggtcgagg tggaggacgt ggtggaggtc ggtgaggtta
     9181 aggatgtata ctccctcttc ggccaccccg gaagctcggc ggggatgtaa gtccccctga
     9241 tggatgcctt ctatctggta tcccccgccg gagagaggct cgacctctcg acggaggagg
     9301 gtagctggct tcccggcttc ggaaggatcg gctacctctc cagcaaggcc gccttccgct
     9361 ggcccggaga ccgcgtcctc gcctccgtcc gggcccccgg ggtctatgat tctggatggc
     9421 acggcgggga gagcagcccg atgctggtca gggattacgc caaggcggtc atctccgccg
     9481 gggagggggg cgagaaggcc tgagacggag gcttccccct ggaggtccag ttcgaagcgg
     9541 ccccttacca ggcgaaggct ggggacggct tcagcgccgt cgccgtcttc aacggcgagc
     9601 ccctcgccgc caactacacc gcctggtact ggaccgtcga cgcccactcc tcccccgagg
     9661 cccagagggg taccgccggc gatgacggga gcttctccgt cctcttggag aaggcaggcc
     9721 cctgggtgat cgaggtggag acgacgacgg acgaggcagg agaatggaac gccacctaca
     9781 gcaaccccta ctatcaggag ggggagctcg tctcctacga gaaggtctgg caccggtcct
     9841 ccctcaccct ctgggtgagg tgatctcctc ccctttttca caccgtctct tcagctccga
     9901 ggcctttttg cccttcagca tttcgatttc atagcaaggc cttgggaatc accccaggaa
     9961 aaccgaaata aaattgttac taaatgttat ttccgaaatc tatttatcat attagtgata
    10021 cttaggcaat ggtgattaaa acgaagagca taattacttt gttgctcctc ctaatcctgg
    10081 gagggatgac gatgaacgcc agcgcccacg ccctctgggt cgagtcgaag gatgtcgccg
    10141 aggtcggggc aactcagccc gtctacttct tctacggcca tgccgggagc gtggcgggct
    10201 tctccgtccc cctgatggac gccgcctacc tcctgacccc ggcgggggag aggctcgacc
    10261 tcgccatgga gacgggcgac tgggtccccg gatacggctg ggtcccctat gggtataatg
    10321 acgtggccct cagcaccccc ggcgactacg tcttcgccgt cgccaggtct ccctccgtct
    10381 acgatccggg ctggggcggg acccaggcca gcaaccccag gctcggctac agctacgcca
    10441 ccatggtgat ccacgccggc gacgagggga aggagagctg ggaggcgggg gtccccctgg
    10501 agatagtccc cgagaaggcc ccctacgaga tcgttgccaa ggataacgtc accttcaccg
    10561 ccctctggaa cggcgagccg gtgaacgcca ccttcaccgc ctactactgg acctgggacg
    10621 cccacggcgg cgacgccgta cagaccggat ccgccgggga ggacggcacc ttcaccgtca
    10681 gcttcaccca ggcggggccc tggcaggtta tggccagcat agacctcaac gagcccggga
    10741 agtggaccgc cacctacgac cacagcgctg gaaggttcaa ggccggggat gaggtggagt
    10801 acaacgtcgt ccggtacacc aacgtcatgt cggtctgggt gaaggcgtaa gccctcccct
    10861 ccatattttt tcttgacggc cggccttcat aagaaagcag ttttttttaa taaataaaga
    10921 tcatgatatt tcatttgagt atcgaatacg gcgtaaaacg gcaacaaacc atataactag
    10981 ccttaaatcc taggacgccc atcccctcgc tgatcagctc agcatccgga tgggtgaagg
    11041 taagagaagg tggtgaacgg atatgagacg gctttatcta tcgcttttgg tggtggcggg
    11101 cctcgtcgcc ctgggctcgg gacacatgct ctttgcggca ttccccgagg aggtcagcga
    11161 agcctcagac gtgaatatat ggatcaccta cagccacgac gctgaagggg agacggcgcc
    11221 ccagctcgcg agggccgagg tgatcggccc cgccgggacg gaaggtctga gcctggccga
    11281 gagggacggc ggcctcgccg ggacggtgga ggtcggcgac cccggctgct acatcctgga
    11341 ctttgagatg gagccgacct tcttcgaccc cgcctggatc ggcttcagcg gagccgcctc
    11401 cttcctcccc aagatgggcc gggcggtcat cccggtcggt tcggcggggg actgcgcctt
    11461 ctcctccggc gaggggctgg agatcgtacc ccagggggat atgggagccc tggggttggg
    11521 tgaccggttc cgggccgagg ccctctggaa cggagagggg gtcggaggag actacgccgc
    11581 catggtggtg aagagccccc aggacgtcct gaccgtcctc cacgcctccg atagcgaggt
    11641 cgaggggacc acctccgacg ggacgatcga gttcgacctc tccagccccg gcctctgggt
    11701 cctctccttc gaggcgacgg tcgaggagtc cggaacctgg accgccacca gcgacgatcc
    11761 cagcggccgg taccgggagg gggagagcct cgactacgac cagatcgccc ccaccgccta
    11821 cctcagcttc tggtgcggca ggtgagcctt tggtccacat ctccgacggc gtcctctcac
    11881 cccccgtcct cgccgcgggc ttcgccctcg ccggcctcct cctcttcgtc accttgaggg
    11941 acgtccgggc ggaggagatc cccaagatct ccctcatcac cgcggcggtc ttcgtcgcct
    12001 ccctcgtcca cttccccgtc ggcccgacca gtgtccacct ggtgatgaac ggcctcgcgg
    12061 ggatcctcct ggggaagagg gccttcgtca gcgtcgccgt cgccctcacc ctccaggcgg
    12121 tcctcttcca gcacggcggg atcagcgtca tcggggtcaa cgccgtcaac atggggatcc
    12181 ccgccctcct cgcctggaag atcttccagg ggcgccggcg ccttccgtcc ccgaggaggg
    12241 aggtggtctt cggcggcctg gcggggggag ccgccgtcct cggcgccact ctgatcttat
    12301 ctgcggagct cctcgtcctg ggggaggcgt tcactgagat cgccgccctc gtcctcgccg
    12361 tccacatccc catcatcctc atcgagacgg cggtggtggg gcttgcggcg ggcttcctcc
    12421 aggccgtcaa gccggagatg ctgatcgccc cccaggggtc tggttacgga ggtgttcgtt
    12481 cttgagaaga gccgtatttg tcgccctcct tctcctcctc accgcgggga tgggggaggg
    12541 gcacaggatg ttcttaggcc accggatgag cctggagatc ttcgccatct acgacgacgg
    12601 cgagcctgcc aggaacgccg atatctcgat ataccggctg aacgggacga cggaagacta
    12661 cgacctctac gaggcggggg tgaccgacgc ccaggggatc tacaaggtga ccctcccagg
    12721 aaaggggacc ggcgagtggc ggtacgccat ctccggagcc ggccactccg aggaggggta
    12781 cttcaccgtc tcggcagaga ggcctccctc cccggaggct aaggctctgg ccctcgccct
    12841 cctccccggc ctcctgataa cgtggagggt gaggaagaga tgaatagggt cgccctatat
    12901 gcggtcctgg cggtcctctt cttcgccggc accgccgccg gccaccggat gctgatgggc
    12961 tattccatcg aggaggtggc cctatccgtc ttttacgacg acggcacccc cgccgggggg
    13021 gtcgccgtcc aggcctacga cggcgatgac ctcatcgccg aagggaccac cgacgccgga
    13081 ggggtcttcg ccttcaggcc ggaggggagg aaggtcgaag acctcctctt cgtcacctcc
    13141 tccgtcggcc atcgggcgga gacgaggata ggggcggctg gcgttgctgc cgtcgagccg
    13201 gagatcccgg tggcgatgaa ggtcgccgcg ggcttcggct acctcctggg gatcgccggg
    13261 atatcgatga tctacgcctc gaagaggagg agataggagg gaggatgggg acgggatcga
    13321 gggttatcgc cgaggggatt gagtcatgag cggcctcgac atagaaggcc tcgcggggat
    13381 cgactctccc atccaccgct gggacccccg ggctaaggtc gcctcgatcc tcgtcctgat
    13441 cttctccgtc gtcatggt
//
LOCUS       DASM01000149           13011 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_3160, whole genome shotgun sequence.
ACCESSION   DASM01000149 DASM01000000
VERSION     DASM01000149.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13011)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13011)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13011
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_3160"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2606 a         3831 c         3799 g         2771 t
ORIGIN      
        1 ggaagtggtc ccgataccgc ctctcagagg gggcggttat cgagatgatg aagctggcag
       61 atctgatgat gggagacgga tggctgccaa attagcgatc ggggagcaga tatatatgag
      121 agagagaggc cggggcaggc ttccggtttt gagcaggttc ctcaccgtat ggatcttcgc
      181 ggcgatggtc ctggggatcg gcatgggcta tctcttccca ggagtctcag aatcgataac
      241 aggatttcag gtggggagta catcgatccc catcgccgtc ggcctgatat tgatgatgta
      301 cccgcnnccc atcgccatcg gcctcatcct catgatgtac ccgcccctgg ccaaggtgaa
      361 gtacgaggat ctgggggagg tctttcggga caccaaagtt ctgggcctat ccttatttct
      421 taactgggtc gtagggccgg tgctgatgtt cgggttggcc atcctattcc tgcgagacca
      481 cccccatctc atggtggggc ttatcctggt agggatcgcc cgttgtatcg ccatggtgat
      541 cgtctggaac gagcttgcct gcggctgcac cgagtactgt gcggggctcg tcggcttcaa
      601 ctcgatcttc caggttttct tctactcagt ctgcgcttac ttattcatca cggtgctgcc
      661 cccgatcctc ggcatagccg agggtctggt ggtggcgata accatggccg agatcgcaaa
      721 gagcgtcttt gtctatctcg ggataccctt cctggcgggg atcatcaccc gtttcgttct
      781 gatcaggagg cgagggatgg cctggtacga ggagaggttc atccccaaga tctctcctct
      841 gaccctgatc gccctcctct tcaccatcgt cgtcatgttc tccctcaaag gggagtacat
      901 cgtcgagctc cccatcgacg tgttgagggt tgcgatacca ttagttatat attttcttct
      961 gatgttcctc ctcgccttct acatatccta caagatgagc gttggatacg agaacaccgc
     1021 cgccctctcc ttcaccgccg ccagcaanng ccatcgccgt ggccgtcgcc gtcttcggga
     1081 tcaactcagg cgaggccttc gctgcggtga taggcccctt ggtggaggtt ccggtgctga
     1141 taggcctggt gaacgtggcc ctttatttaa aagatagata tttccaagga gtgtacaccg
     1201 aatgctaaga aaactagcga tctatggatc agttctactg attgcaacgg ccctcctcag
     1261 cgatgcagag gcgggatgcg gctgcagcgc gggaagcagc agctcctgga gcggacccga
     1321 ctggatagat tcgacctcgg gcggatcctc tccgaagcca ctcccgtcga atgggtcggc
     1381 tcccgaaacc gttgacgaag gaactcacgc cgaaaaatct gagggagatg aaaacccgga
     1441 gatcggcatc tcattcgtat ctgccgagga gctgggcgaa aagctcggga tcgagccaaa
     1501 gcccgtcatc gcctacgtca gcaacagccc gccctcaggc ggctcgtaca tcgggggatc
     1561 gatctcgctc ccatcaaagg ggttcattca ggccgacggc tccctcaagt ccgtggatga
     1621 gctggcgggg gtcctcggaa gggccggggt caccgctgag gacgaggtgg tcctctacgg
     1681 ggactgcttc tcctgcggcg atttcacctt cgtctactgg atcatgaagt acctgggcca
     1741 ccagaaggtc gagatcctcc ggggtccgac ggccgggctg cccacggccg ggtccgtagc
     1801 gacgaggcca gcggctgact actcgccaga tgttcacccc gagcttctcg cagactacga
     1861 atccgtcgtc tccggccagt ttgtggtcgt cgacgcgagg acccccgacc agttcggaga
     1921 aggtcacatc gacggagcgt tgaacatcga cttcaacagg gtgatcgatg gcagctggat
     1981 caaggatgac tcagctcttg ccgagatctt cgccgacctc gatcccgatc ggcccgtcgc
     2041 cgtctactcc aagaacggtg gcacggcatc catcgtctgg tacgccctga ccctccaggg
     2101 gcgagacgtt aagctctaca cctggaacga ctgggtgata cataaataac ccagtcaatt
     2161 aaaaataaac gtgaaaaaaa attatttttt tgtgatcaga cctcatctcc gatcacgcgt
     2221 cccagtcgcc gacgttctcc tcatgatagt ctatggcgtc ctcgacgcag gataggagcc
     2281 agtcctcccc cgtctcgccc tcggcgctct tccagccgac gacgacgttc ccatccttct
     2341 ccaccagggt tattatcaca gtcaaaggta ccaacgactt ttttccgttg ggatcgtaga
     2401 cctcaaagag ggcgttggct ctttcgttgt tcgggtctgc gatcaggttc tcgaaggcta
     2461 tggcgtcttc accgacctcc tccaggacgg cgttggcggc ttcttcctga tttatgcagg
     2521 gaatgcagtt ggtgctgtgg tccaggatga ggatcggccc atcttccagg agctcgagga
     2581 cccatgaggg gtggtctacc tcagagccag cacccggatg gtggtcgggg tagacggtcc
     2641 accagtcgtc ggggtcgggg cccaccaggt actctgctgc cgagacggcg ccgatgaggc
     2701 cggcggcgag gacgagccct gccaccaggg ccgcaaggtt ataactgagc ggatgcgaaa
     2761 tatctaccaa ctccaattat cgtttattgc cccacttcgc ttctcacacc ttttatctcc
     2821 agcggcaatc ttgggcagat gtatcgacga tcatggatct ctctttcacc tcgattggcg
     2881 ctcatggctg gattgatccc ccggtggact atgttgatat ataaagctgt tggaaattct
     2941 acgatattat cttcaataat caaattgaag ccccccattt ccccgtttta accttccata
     3001 tggcttataa atggcatcat accgatcacc gccaccggcg aaaggaagga gctccaggag
     3061 catcgatata cttatatatc gaattagctt gatatgaaag ctgaaagccc cggagaggac
     3121 atgaggtgcg atatctgcgg cagggaaacg gagacgctct tcaaagcgag acatcgggat
     3181 aggggcgggg tgaagatctg cgaaggatgt ttgagacggg agggggaacg gctcgtctcc
     3241 tcctccgggt gctcatgctg ttgagccgat ggatatgagc gacatcgtcg aaccccagga
     3301 tccctccatt gagcggctgg cgaggctgat gggggatcgc tgtaaggcta agaacgctag
     3361 caagaggctt cagaccctca ccgagaagat agacgaggcc cggatagcgg cggaggtcga
     3421 ggtcttcagg gcgatggcag accccttcag gcttgcgatc ctccagctcc tcagggaggg
     3481 ggagctctgc gtctgcgaga tcatgaccgc cctcaacagg ccccagtctt ccacctctca
     3541 ccacctctcc atccttaaga gggcggggct ggtgaaggag aggaaggagg ggaagtggtc
     3601 ccgataccgc ctctcggagg gggcggtgat cgagatgatc aacctggcgg ggctggtggc
     3661 ggagggggga tgaggcatga gccgaacccg gttccaaggc cgcccggtct cttggagggc
     3721 ataaaagatg cgaacggatc atttgaagtg cggatctctt ctattggtgt tgatggctct
     3781 tctcggcggc gctgaggcgg gatgctccag ctgccgcggg ggaggtgacg ccacctgggg
     3841 gggcgaggag tggatggctg agaccctcgg caggtcgtcg aacgggtccg tttctccgat
     3901 ggtggaggac tccggggccg acgggggctg ggcggcagag ggatcggagg gcgagccgtc
     3961 gccgtcgttc atatccgccg aggagctccg ggagaggctc ggggctgaac ggaggcccat
     4021 catcgcctac gtcagcaaca gcccgccctc aagcggctcc tacatcggcg gatcgatatc
     4081 cctcccatca aagggcttca tccaggccga cggctccctc aagcccgtcg aggagctggc
     4141 agcggtcctc gggagggcgg gggtcaccga ggatgatgat ctcgtcctct acggagactg
     4201 cttctcctgc ggcgatttca ccttcgtcta ctggatcatg aagtacctgg gccaccagaa
     4261 ggtcgagatc ctccgggacc cggcggcggg gcttcccacg gccggatcgg tggcgacgag
     4321 gcccgccgct aactactccc ccaccccccg tcccgagctc ctcgcagact acgaatccgt
     4381 cgcctccggc gggctcgtcg tcgtcgacgc caggacccct gaccagttcg gaggaggtca
     4441 catcgatgga gcggtgaaca tcgacttcaa cagggtgatc gatggcagct ggatcaagga
     4501 cgatgccgcc ctcttcgagg tcttcgcccc cctagatccc gatcgcccca tagccgttta
     4561 ctccaaaaat ggtggcacgg catccatcgt ctggtacgcc ctggccctcc aggggcgaga
     4621 cgccagcctt tactcctgga acgactggct gaggcaccgg agctgaaaag gacgggcgaa
     4681 agaacggtcc atgggtgttg gggagggcgt atcagccctc cagcgtagag aggaaaacct
     4741 gcccccgcga ggaggtcggc atcggcgggg gcccacctcg tcccccctta tcctaagccc
     4801 ctccctctcc ccgtgccgcc gtcgtcatcc ggataggttc atcagcctcc tggtgagccc
     4861 ctcaaaggtt ctctccaggg agctcatgta aggtctgtcc acctcctttc ggctcatctt
     4921 atccgagaca aaaggcctat cttcgaccca ggggacgtac tcccacttcc aaaggtccgg
     4981 gtttgtatcg tgctcggcca tggcgtggtt ggaggtgttg acgaagatta tggggtgtct
     5041 gtactcgtcg gcatagtatc taacccggtg agggggaaga tccggttcgg ggtcgccgtg
     5101 gatcgtgtcc tcttccaggt tgtggtggcc gctgtagatc ttggtgaatc ggagaccgct
     5161 cgctttctca tcaggggagt ccgccatgat gacgaagctc tcgatgtcct tcaggcggcc
     5221 gtatttggcg agccttaccc gttcgtagat cttattgagg accctatgct tcctcagctc
     5281 ctcgttattg aagaccaggg tcacctccat ctccatcccc tcctccgtcg ggggcctctt
     5341 aaagcagtgg atctcccgga tgaagttctt cataccgtcc accgccggct gatatatgac
     5401 aggagagcat tcatcattct gccctacatt gaggttgaat ttgttggtgt acaccagctc
     5461 gtctgcctct aaactatcat ttatatattt atacacatct cctatcgtgg ccttctggtg
     5521 gccgcccgat gatccaggcc cctcctccgc ccattgccaa atccaagcgt cctccccgat
     5581 ctggaaggac catctcatct tcgggccgat cattatccat cgatctttga cgtgatctcg
     5641 gaccttggcg gtgtagtagt cggtctcgat cttgcctatg cccttatcga tattgatccc
     5701 cagcatgtac ctaagagttg catcgaaggg ttccccgtcg gagtcgatct tgtcgggccg
     5761 accgtatctg gtctggacct ctcccctcct tctcagtatc agcttcttct tcccgtcctt
     5821 ggtcctgacc agctccaccg tggtgaggtc cctccagtcc cacgtcctct gctcctccgt
     5881 ccatcggaac tctcccaaat ctgcagaata ttctcgctcg attttagaga accacagccc
     5941 ttcagcatcc ataaaactca ccccgataat accaaccctt ttaacaagtt aaatgtttcg
     6001 attgttcagc tcttcccggc cggcaaggct cattaaaccg tcaaaatcaa aagcagatgt
     6061 ggcagttaaa tccggaattt ttgggcacct gatcggatgg gtctaccgcc tgcggcatcc
     6121 ctggagacgg gaggcggaat cgggtgggcg tcgggggatt ccgggcccta gatcagccaa
     6181 ccgatccgtc ggttctgaga tgatggtcct gagatctctc agatgttctt tgagagcttc
     6241 atcagcttat cgataaaggc gtttgtggca tatcctacga taaagcatac cgccatcctc
     6301 atattgtcgc ccactgccgc ctcgaagctc tctaccttct gggctgtgag attggcggct
     6361 ccagtggccg tcaacgtcgt atcgttcaga aggggttgct ccagcccgaa gagggtcagg
     6421 tttccggtta tattcgaggc ggttaaagat acgccatcac ccgccacgac cacctctccc
     6481 aggtctgtca ggatgtaggc gatgaagccg aatatcgctg ccaagaaggg gagggcggta
     6541 taccagaccc gcttataatt ctggacgacg ccagtcgcct tgatatcgtc cacggttccg
     6601 accatgatct gggccgatgc tcccagccct gcgatggcgg gagcccagag ggggaccgtc
     6661 tccaggacat atttattcat attactgtat aataatatgt agccgaagaa aactgccccg
     6721 atcatcccat aagataggga aaataggccg tacttagcca taaaaaatga tatgtaggtc
     6781 catttcggaa agctcaggag gtcatatttc tcatgattga gggccgcggt cagctcggag
     6841 ttggaccgat cagagatctt catcgactct agggcattct tatcccggta aaggctctcc
     6901 cgagagctat tataaaattc tttggcactt ttgacgtcta agcccctcga ctctgcaatc
     6961 tctaaaagca ttcctagctt cgtcagacgc ccttccgcgg cttccctcat attcacttca
     7021 tccttcaatt cagagatcga agggcctcca tcgtccctgg actccttctt cttgaaactt
     7081 ttttcgggct ggtcgctggg atcctcttcc ataccatact ttcaagttta ttccttatat
     7141 acaggtttcg gtcttattaa tatatattat ccttagcgag gatttcgatt tactcatttc
     7201 tagagcactt tttatcccta aatccaatta tcaggatcat ttttccctgt aaatctccaa
     7261 attttctcag tttgcgttct tttctcgacg attttagata gcatccgcta tcctcaaatt
     7321 acattctcct tttttagtgt atgtaactga tacagattgt actcgtttca gggtagtaac
     7381 aagaaccctg ccagctccaa agatccgttt tataaccgcg aagggcctct cgccaggtga
     7441 tctcagctta cttatcagcc gattccattc cttttcgaga tcatccaacg gcctttctgt
     7501 cgtccttctc ttcatggtga aatcttcacc cctcgcaggc acgccaaaat atcccttatc
     7561 tctcagcacc acctctcctg ggacagaaag atcgaccttg ctatggtgaa ctttgccgtt
     7621 gtggtaccta tctcacggat cagtgcatag tctatatata ctttagaatg aagtttatat
     7681 ccgaaatgaa attcatcacc tttcctggtc catgtgccat ctctgctttc ggcgagtttt
     7741 agcctgatac cccccgtgtt tttttcgatg aaccaggatc agcttcgatg aaggtggcat
     7801 cctggatcgt tccaggtttc acagttagtc ccttagaatc caattgacgt ctgaactcat
     7861 cccagactcg atggtgcgtt cctgtctcta tcatccgctc cttgaatagc catatcgtgc
     7921 tagaatcagg gacctgctcc ggaaaaacaa gaaatcccat aaaagatact cgatctgcga
     7981 tctgtctcat aatttcaaga tcgtttagac cgtaccactc ctcaagtatc agaattctaa
     8041 acatcatcag gacgtcgcaa ttaggccggc cacctttctc gcttttgttt ttgtacatct
     8101 cctcaagtag cttgcgaagg ggcttccagt ctatgatatc aactatctgc aagagccgat
     8161 tcccagattt ctggttactg taaacttctc ggaaagttca ggctgcaaat gtattcatga
     8221 tacttaatta taagtaatac tatttaaata atcttatggt taggtttttc gaaatcctct
     8281 atcttcttac cgtaagaatc ctgttggtca tccccctggc cagagaataa gggacggtga
     8341 ggaggaatac gccgacgatg atcgccggga tgacgcggac ctgatcgaag atgaggtatt
     8401 gagcgatcat atcgaagacg gttgctatga tgatcgtctt ggagagagac ttgagaactc
     8461 cagcaagttt agtccttcgg ttctcgcggt cgatgatcag atcaatgatg aatggcggtt
     8521 cgcccgcctt tgcatccatc gccccgtcgc gtatccccag cagcaccgct accagtggtt
     8581 gaaatacgaa tcgaaggctc atcggcccga cgagacgggc tatgatcctt tcggctacag
     8641 catccagcaa tgaggtcgtg cttcctcctc cgtccttgcc cgtatatacg tctttagctg
     8701 gcaggttctg gctcatcacc gttaacgatg aggagtataa ccgccatcaa cgccattccc
     8761 gccgctgcct caggcgggcg gatcatctcg ccactccaaa aagccatgcc gtgaagcacc
     8821 cggatgatcg gtcccggtgc gccctggacc aagccctgcg ggggtacgcc gaaacggctg
     8881 ccatcgccgc gggcccgtcc ggcttgaggc cgggaaaagg cttccagcgg ccggatccgt
     8941 cagatccacc tatccacacc ccccccgagg gtccccggtt tgaccggaga agctttcaat
     9001 gatcgtcgcc gaaaaaagcc aaatagcacc atgatcgacc ctgaccccct cccccacctc
     9061 cccggatgct acctcttcaa ggacgacgag ggagccgtca tctacgtagg aaaggcccgg
     9121 gacctgaaga agagggtcgc cagctacttc cagaagaggg agctcgatcc caagacctca
     9181 gccctcgtct cggcggcgag cggcctcgac ttcatcgtca ccaagagcga ggtggaggcg
     9241 ctgctcctcg agaacgccct catcaaaaag cactggccga agtacaacgt ccggctgaag
     9301 gactcctccc gatacgcctc gatccacctc accgacgagg agtttccccg aatccacctg
     9361 tcccggaggc ggaccggcgg cggcagctac ttcggcccct tcgtctctgc gagggagagg
     9421 gacctcgtcc tctccgccgt ccggaagacc ttcggcctgc ggacctgcaa gcggcttccg
     9481 aagagggagt gcctccgcta ctccatggga gcctgctccg gcccctgcat cgggaagatc
     9541 gcgcccgcgg agtacagggc gcgggtggcg agggccgagg cggtcctgcg cgggaggacc
     9601 cgggagctga tcggcacgct gaaggaggag atggcggccc tctcggagcg gcaggagttc
     9661 gagcaggcga tggagctccg ggacgagatc cgggccctgg agagcctcca ggaccgccag
     9721 cagatcgagc gggacaagag gtacgacgag gacgtcctga gctggatcgt cgatggggat
     9781 accgtctacc tgatgctctt caaggtctac cgggggaccc tggagggaaa ggaggagcac
     9841 gtcttccccc ggaccgagag cttcctcgag gagttcctcg tccagcacta ctccgaggaa
     9901 acgcctcccg aggagctgat cctgggtgaa gccctcgacg actccctcgt cgacttcctc
     9961 tcccacatca aaggaagacg ggtccgggtc accgtcccga agcaggggga gaagaggaag
    10021 ctcctcgacc tggcggagaa gaacgtgacc gtccggttcc tgggcgacaa aaagaagctc
    10081 ctcgccctcg ccgaggcgct ctccctcccg gggcctccga acgtcgtcga ggtcttcgac
    10141 gtctcccacc tcgccggaag cttcaccgta gggtcgatgg tccagttccg gggcggaagg
    10201 cctgacaaga gggggtaccg ccgcttcagg gtgaggaccg tcgacgggat cgatgacttc
    10261 gcctccatcg gcgagatcgt caggaggcgc tacctacggc tgaaggagga ggggggggag
    10321 atgccagacc tgatcgtcat cgacggcggg aaggggcagc tctgggcagc cactcaggag
    10381 ctgcgggggc tcggcctcac catgcccgtc atttccatcg cgaagcggga ggaggagata
    10441 tacgtccctg gccgcttccg gcctcttccg atcaggaggg acgagaaggc ctccctcttc
    10501 gtccaggaga tgagggacga ggcccatcgt tttgccatca cctatcatcg actgctgaag
    10561 aagaaggcta tcgacgccta gcccccggaa gggagggcga tccgaacttt tttggcctgg
    10621 gaggagagat ctggctagag agctgaagct attacaagtt cgaggcggtg gagttgttca
    10681 ggtttgcgag agatcaagag gtggtgaacg tcgccggggt gaagataggg gggcagccgg
    10741 gggagaaccc gacggccctc tgcgggacga tattctatca gggccagagg ctggtggagg
    10801 acccggtccg ggggaggttc gacgaggggg ctgccgaggg gctgatcgag ggacagactg
    10861 ccctctcgga ggagacggga aaccccgccg ttttgcatat ctacgccagg acccgggagg
    10921 ccttcggcag atacctcgac ttcgtcgacg gcctctggtc cggcccgctg atcctcgact
    10981 ccgccgaggc ggggacgcgg atcgccatgg cggaggctgt ctcggagatc ggcctcgctg
    11041 acaggtgcat atacaacagc atcggcatcg ccaccacccc ccaggaggag gaggccctgg
    11101 cccacggcga ggtcgactcc gccatcctcc tcggctacag ccccgccgac tccaccgtcg
    11161 aggggacgat ccgggtcctg gcggaggggg gtccggggcg gaaggagggg ctcgtcgacc
    11221 tcgcccgccg cctcgagatc aacaacatcc tcatcgaccc gggcgtcgtc cccctggggg
    11281 acggagcggg gaggtcgctg cgggccaccg tcctcgcgaa ggcgacgctc ggtttgccgg
    11341 cgggctcggg gatccacaac gccgtctcct cctggcggtg gctccggtcg aaggagaggg
    11401 aaacgaggct cgtctgcgac gccgcctctg caggcttcga gatcctggcc ggaggcgact
    11461 tcgtccttta cggccccatc gagcgggcag gggaggtctt cccggtggcg gcgatggccg
    11521 acatcctcgt ctcggaggcg gtgaaggacc tggaggtctg gcccgccgcg ggccacccca
    11581 tcgggaggct gatctgaccg cccgatctga gcctttttat atctccggcc ccaggggagg
    11641 ggcatgcatc tatcgcggga ggaggagcgg gcgcaccagg gcgagctcgg cgatagcctc
    11701 aggaaggcga tggagatcct ggtggccctc ggcgacatct acggagccga tcggctgatc
    11761 cccgtcaggt ccgtccaggt ggcgggggtc tccttcaaga cgatcgggga ggcggggctg
    11821 gagtggatct cagacctcca gggcaaggta gcggtccccg cggtcctgaa ccccgcgggg
    11881 atggacctcc accggtggcg ggagatgggg atagacgaga gtttcgcaaa aaagcagctc
    11941 gaggtcgtgg aggcctaccg ccgcctcggg gtctcccccg actgcacctg caccccctac
    12001 cagatcaagc ccggttttgc ccagaggggc gaccacctcg catggagcga gtcctccgcc
    12061 gtctccttcg caaactccgt catcggggcg aggacgaacc gggagggggg gccctcggcc
    12121 ctggcggcgg ccctggtggg gaagaccccc cgatacggct accacctgga ggagaaccgg
    12181 gtccccacca tcaatatcgc cgtcgatgcc gatctcaaac cttccgacta tggcatcttg
    12241 ggcttcatcg tcggaagaga ggtgggcgag ggggttccgc agttcatatt ccggaggagg
    12301 cccgatatcg atgagctgaa ggccctgggg gcggccatgg cggcgagcgg ctccgtcgcc
    12361 ctctactact tcgaggaggg ggggccggag gccccgaagg gggcggagga gatcgaggtc
    12421 actgccgcgg accttcaggc cgtccgggag gagaactccc ccgacgagga ggtggatatc
    12481 gtcgccatcg gctgccccca cacctcggag agggagctgg aggagctcgc ccgccttctg
    12541 gaggggaaaa aggtggaacg ggagctctgg atctgcaccg cccgggggat agccgagagg
    12601 tgcccgcgcc tcgtcgagag gatcgagggt tcgggggcgc gggtcttatg cgacacctgc
    12661 atggtcgtct ctcccgccag cgaccagtac aataagatga tggtaaactc aggaaaggcc
    12721 ctagcctacg tccccaccct ctgcaagatg aaggcagcct acggcagcgc tgctgaatgc
    12781 gtcgaagctg ccaccaggag ggtctgacca tggagtttct ttgccacaaa atatccggcg
    12841 gatcggcctc ggcccccgcc ctggcctcga gggaggcgat atccttcctc ggcaccgtag
    12901 acccggagac gggagaggtc gtcgaccccg gccatcagct cttcgggaag gccatcggcg
    12961 gcaaggtcct gatcttcccg ggaggcaagg ggtctaccgt cgggtcctac g
//
LOCUS       DASM01000150           11563 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_118384, whole genome shotgun sequence.
ACCESSION   DASM01000150 DASM01000000
VERSION     DASM01000150.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11563)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_118384"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    11414..11442
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1982 a         3774 c         3709 g         2067 t
ORIGIN      
        1 tcccatcggg aagggggacg tcatactcct cctcgacgcc agcggcgggg gtcccaacac
       61 cgcagacctc ctcatcgagc tgggggtgga ggcggtggtc gtcgagacgg agatggagcc
      121 gtcggtggag gggtatctga tggacggagg ggtccccgtc ctatccagcc gggaggtgcc
      181 ggtgcggagg gtctccggga tcgccctcgt cgaccccgag gatctggaga gggctcgggc
      241 tcagtggtcg gagcggaagg cggtccggga cgcccgacgg aagaccgagt ggctggaggg
      301 gatgatccag gagtaccggg cggagcggag gaaggacgag cgtcagagga ggaggccctc
      361 tccggagtga ggagccgggc cttcctttaa cccagacccg ccctcctccc tctggggcga
      421 ggggagcgtt tttaaggata acccgccctc actgccccca accatgctcg ccgacgatat
      481 actgaggttg aagagggagc ggaacgccgt catcctcgcc cacaactacc agcccggaga
      541 ggtccaggag gtggcagacc tccggggcga ctccctggag ctctcccggg ctgcggcgag
      601 ggaggaggcg gaggtgataa tcttctgcgg cgtcgacttc atggccgaga ccgcggcgat
      661 cctctccccc gacaagaggg tggtgatgcc ggcggcggaa gcgagctgcc ccatggcctc
      721 catgatcacg gcccgggagc tccggggctt caaggaagag aactccgggg ccgccgtcgt
      781 ctgctacgtc aactcctccg ccgaggtgaa ggcggagagc gacatctgtt gcacctccgc
      841 caacggggtc gaggtggtgg cctccctcgc ggaggagacg gtcctcttcg tccccgatag
      901 gaacctgggg aggtacatcg cccgcttcac cgataagagg atcataacct ggaacgggta
      961 ctgctacgtc cacgacaggt acaccccgga ggacgtctcc agggcttgcg ccctccaccc
     1021 cgaggccgag gttctcgtcc accccgagtg caggcccgag gtgatcgacc tcgccgacgg
     1081 cgtctacagc acctcgggga tggcgaggcg cgctaaggag agtccgtccc gagagttcat
     1141 catcgggacg gaggtgggga tgaactacag gctgaaggag gagaacccgg ggaaggagtt
     1201 ttaccccttg agcgaaaagg cggtctgcct cgacatgaag aagacgaccc tggagcgggt
     1261 cctatccgcc ttggagaccc tggagccgag ggtcaccgtc cccgaggaag tggcgggccg
     1321 ggcgagggtg gccatcgaga ggatgctctc gatctgattc cccctctccc ggaccaatgc
     1381 ttttataggg agaggccttt accaggacaa tcgcttcccc ctcgccagga ccctcgaccg
     1441 aagggagttt tgagatgaag gtgggagcaa ggcctggtga tcaaaatcag cctgaatatc
     1501 aatgctcgtc agctccggta tcccctcatt gcgggcagcg tcctgctgct tttagccgcc
     1561 gtgtacagcg atgctaacca gttgaccatc gagccgttga tagagctttt gggggtggtc
     1621 ctgatcctca tcaccgccgc catatggaga aggtgatcta gcgagagttg tatccgtctc
     1681 ccttaaacca ttgatatccc attttccatt tcgaccttag aaggccgatc cgccaccaaa
     1741 aggctcaggg ggtcttcttc tcgaaagcgg gctctgcctc cccctctcct ctcttcgccg
     1801 gtctagccgg ccttcccccc tccccgccgc cctcctcctg gaggtcctcg agggcgtcgg
     1861 gaagctcagc cccgcagatc gcgatccctc ccttcaatcc catcccccgc taatccgact
     1921 gctcccgggc cgcctgcgga atgctcccac ctccggaggt ctcgtgccag gccagccgag
     1981 atccccgccg cctcctccat ccgctggcgg gtggcgtgcc cccgggggag gacctccgac
     2041 ccgagccagt cggcgaggtc gtcgaagaag gcggcgagcc tcccggtggc aacggcctcg
     2101 gcgaggccga gggagtcgat gacgcattcg gccgcgacct ctccgccgac gagccagcgg
     2161 gccctggccc ccagggcgtc gccgaagggc ccgccgtagg tgagggtgag gtgccaggag
     2221 gcgcccggcg atgatccgca aggaaggtcg atcctgaagg gcagggggtc ctcgcggtcg
     2281 aaccggtcgc actccatccc gccggggccg gccgtgacga cctcgatcag gagaagctct
     2341 ccgatccggt cggccctcct tctgatctcc tcctccaggg ccaggatcgt cgggagggat
     2401 ccggcagttc tgtctctgtc ttctcttgtc ggggtctctc tcgcatcgca gataattagg
     2461 cctcctctcg gatctcttct cgggcttgtt tggaatgggg tggataaagg cttgcccatc
     2521 ggcttgagca tcagttcccg tcttcatgag ggtgggtcag ctcggtatgg gccagatcgg
     2581 gatggcgatt tatatcataa cgggagattc caaatggtcg tcgtcatgtc ggaggatagg
     2641 agccgggatg aaatctggga tctcattgtc gtcggagcgg gccccgccgg cctcttctgc
     2701 gccgccagcg cctcaggggg gggccggaag gtcctcgtcc tggagaagaa aagatacccc
     2761 ggccgaaagc tcctcatctc gggatcgggc cgatgcaacc tcacccacga cggcgaggcg
     2821 agagacttcc tcggccacta cggcgaccac ggccgcttcc tccgcccggc catcctcgga
     2881 ttcaccaacc gcgacctcgt cgccttcttc gaggaaagag gcctctccat gatcaccctg
     2941 gagggtggga agatcttccc cgagacccag aaggctcggg acgttctgga catacttctc
     3001 gcggagtgcg aggcccgggg aatcgagatc gccctcggta aggccgttac ctcgattgat
     3061 aaatcggggg atgggtttct ggtcgcctgc ggggatgaga cccgtcggtc acgcacgctc
     3121 gtcatcgcca ccggcggccg gtcgtatccg gccaccggct cttccggcga cggctaccga
     3181 ttcgccgagg ccctcggcca ttccatcgcc gaggtcggcc ccgccctcgc cccggtccgg
     3241 atcagggatt atcccttctc cgacctcgcc gggatctccc tccccggcgc cagggtctcg
     3301 atattccggg ggcggaaggt gaaggaggag gcgggggacg tcctcttcac ccacgatggg
     3361 ctctcggggc cggggatcct cgacctctcc agggatatcc gggccgggga cctcctgaag
     3421 gtatcttttg caggggccag gaaaatggag gagattgaag ggtggcttct cgcgaggtcg
     3481 caggaggagg gcggccgaaa cctgaggtcg atcctctcgg agctcgcgat ccccgcgagg
     3541 ctcgcgtcga gggtctttga gatcctggat atccctcaag acctcaaatc tgcctccgtg
     3601 acgaggagga tgaggatcga tctcgccgac cgtctctccg gctttccctt cgtcgtcgag
     3661 gaggtcggag gcttcgattc agcgatggcg accaggggcg gagtcgattt gagggaggtg
     3721 gactcgaagt cgatggagtc gaagctggtc ccggggctct acttcgtcgg ggaggtcctg
     3781 gacgtggacg gggataccgg cggctacaac ctccaggccg ccttctcgac gggttttttg
     3841 gcggcagaga ggatcagaaa gcggcttgca gaattggagg acggaatgtg aaccaggggc
     3901 atggtctatg tcggagaacg agatcatcga cctgagacgg catggaaggc cgccctacgc
     3961 cgtcgccgtc atccacggcg gtccgggggc ccggggggag atggcgccgg tggcgcgcgt
     4021 cctctcttcc agccggggcg tcctggagcc gctccagagg gcgacctcgg tccgggggca
     4081 ggtggaggag ctccgaaggg atctggaggc ggcgggggac ccgccgatgg ccctcatcgg
     4141 cttctcctgg ggcggatggc tcggcttcat cctggcggcc gagcatcccg acctcgtcgg
     4201 aaagctgatc ctcgtcgggt gcgccccctt cgaggagagg cgcgcatccg ccgtccacct
     4261 caccaggatg agccgcctcg gggtcgagga gaggagggag gcctcttcta tcctcggcct
     4321 tctggagggg cctgatgtgg ccggggcgga ggagatggac gcggccctcc ggaggctggg
     4381 ggcgatcttc tcgaaggccg attcgtatga ccccattata tccgaatccg tcgacgtcga
     4441 cctccagccg gaggcgtttc aggcggtctg gagggaggcg gaggagctga ggaagagcgg
     4501 ccggctcctg gagctcggaa gaaggataga atgcccggtg gtggcgatcc acggggagca
     4561 tgacccccat ccggccgaag gggtccgcct ccccctctca tcggttctca agagcttccg
     4621 cttcttcctc ctggaggaga gcggccataa gccgtggatc gagaggaggt ccagagacaa
     4681 attctaccag atcctggatc aagagctgag atgacggcct ctcctcaagg ctaaagaccc
     4741 tccctctaga taggcatcat ggaagcttct caaagaccga tcgccacctt tttctctata
     4801 aaaaaaagat gtatctctca tgccgcgagt gatccatttc gagatccccg tcgacgatcc
     4861 ggagcgggcc gtcaacttct actccgaggt cttcggctgg aggctggagc gctgggagcc
     4921 cttcgactac tggctcgcct acaccgggga ggggcccggg atcgacggcg ccctcaagat
     4981 ccgggagagc cccggcgagg ggacgacgaa caccatcgag gttccagacc tggaggaggc
     5041 gatggcgaag gtcgctatag aaggggggga ggtgatctcc gagatcatga cggtgccggg
     5101 ggtcggccgc ttcgcccgct gccgggaccc ggaggggaac gtcttcggga tcatggagat
     5161 ggacgaggcg gccgagtgag gccgcccctc cccaggcgcc gcccctccgg gggcttttcc
     5221 gtcccgggga tctctccaac ccctcataat accgcatccc ccccgcctcc cgggaggggg
     5281 cgcggtcgga ggttcaatgg cggcgtccgc cgatggcgac ttccctaaaa cgctcagtac
     5341 ttctcgagga accacttctt gtcgaactct ttgatctctc ggaccgtcgt ctccggcttc
     5401 cagaccacca ccatggcgat gtctctctca atatcgatca tgttgtagag ctccaccttg
     5461 acgaaggcgg gaacggtgta ggcggtgagg gcgaccttct ttgcgtcctc gacgaactcg
     5521 tcgtaattct cccttcggat tatgtaggtg ctgtcaaagc tgtcctccaa cttcttcacc
     5581 tccagaggat cattcgcccg aatctactaa aactttttga tcagatcttc tcagattttg
     5641 cccctcgacc agagccgccc ttcctggctc ctcaggccgc ctcgtcctcc tcctccctcc
     5701 agggcgggga gacgaggcag aggaagacga gggggccgcc cccgacgttc ttgatatgct
     5761 ggattgaacc cggcgggatg tacaccgcct ggccgggtcc cacatcgcac gattcgccgt
     5821 cgatctccat ccttcccctc ccctccagga tgaagtagac ctccgaggag ctcttcagcc
     5881 ggtggggggc ggaggcctcg ccggggccga gccgggcgtg agcgaggctg tagcccatgg
     5941 atatctcctc ctccctctcc gggtggagga gctcgcagat atcggtcccg tcttttgctc
     6001 tggaccgcag gctttccctc aactccctga tcaacatctt ttcaccctac cattccatca
     6061 gaaaatatcg ggactgcact cggcgaggaa ctcgtcccag gggccggggt atctgcttat
     6121 gaactcggca aggagctcct tgggctcttg aaggtcgagg tcgacgactt ctataccgtg
     6181 ggcctccatg aaccccctcg cctgaggggc gctctcggcc tcccccgcca cgacccgctt
     6241 tatcccgaac tggaccgtgg ccccagcgca gaggtagcag ggcatcatcg tcgaatacaa
     6301 tgtggtgccg tgatagccgc cggtgagcct cgagcggcga aggcagtcga tctcggcgtg
     6361 gataagctga tcctcctcct ggacccgccg gttccggcct cggccgatga tcctcccctc
     6421 ctccaccagg acggcgccga tgggaactcc cccctcggcc agccccgccc tcgcctcctc
     6481 catcgcctcc tccatgaact cgaggtctat cttcatcacg ccctccgtct tatcctccat
     6541 ctcgccgccc ctccgcctca acccccatcc acctctcctc atgggctgga tgggccccgg
     6601 acctcctccc cttcctcgac ctctgccccc atccggacca ggtccgcgag gagcctcccc
     6661 gggtcgacga tgtggcccat catcgataag cccgcctttc tcgccgtccc gtccaggatc
     6721 tggaggagga gggcgacgac gggcatcgct gtgaagccgt agacgtcgtc gtggcctgcc
     6781 ctcaggcgga cgaggacctc ttcgccgccc ctctccccct ccgcctccag gacgacggcg
     6841 accgcctccc ccggcggtgt gaacctcttc gacccccaga ccagaagacc ggcaagccta
     6901 tctctacccg ggcccgactt gaccttgccg aggaggtagg cgaggggggt caccaccccg
     6961 tcgacgaacc agtttatccc ggctacgtag gcccccagct cctccacccc caacatctcc
     7021 ggaaggcccc tcatctccgc cagggtcgtc ggatagcagg tcttcacccc caggggcggg
     7081 ccgaagtcga ccttgcggac ctcccgggcc cccgctcccc tccaccggcc gtccctaaag
     7141 acctcggcgg agtattcgcc cagagcctcg acgagctcca gggcggcacc gccggggtcg
     7201 aagtcggtcc tcatggcaac agaggcggcg gccttcctgt atctggtgaa gcggccttcg
     7261 gcagcccgag cgaagaccga agggagcccg ggaagaaatc ccgcctgggt gacgaaggcg
     7321 aggccagccc cctcgatctc ggggcggagc ccctcgagga ccgccacccc ggcctggggg
     7381 tagtggatgt cgatccagtc cgcccccgcc gctaaggcgg ccctcgccgt cgtctcgatg
     7441 aaagggacgg tcgtcgccgc gtcgatgacc aggtctgcgc cctggaaggc ctccctcagg
     7501 ctcggggggt cggaggcgtc ggcttgggca taggatgctc gatccccgga gaactctcgg
     7561 ttcagagcct ccgccaggcc gctggccctc tccttccgcc ttcctgcgac gatcaggtcg
     7621 gcgggggtca tcctcaggat cagcctcgat atggcggagc cagcgccgcc gtaaccccca
     7681 aggaccagga tggaatctat tgtcaaggat cgacctcccc ttccgggctt tcgtccgttg
     7741 gtggcaggtg taatcgggga tgggggcgat gaccccccac gtattttcgt cctcctggag
     7801 taaagggatt tcggatcttc cgtcaggatc cgccctcgcc tcttcctcgc ctgagcctat
     7861 cgtttcccac ccaatagatc cgcccctgga ggatggccgt cggagcttcc gcccttggtt
     7921 tctagagggg ggtaagctta atagatgccg atcgataata gaagctgatg atagacctct
     7981 tccttaaagg ccaaagaaac ccccacttta ccggccgaag ctcgattttg agagatctga
     8041 aggagcggct ggctgatgga cccgtcctcc tcctcggtcc cgacgggatg ggtaagagcg
     8101 ccatcgccga agagtacggc cggatccact tcagcgaata tgaccacagg ctctgggtca
     8161 gggcctggga cggggcggtc ctcgcctccg actacgcctc cctcgccgag cccctcggga
     8221 tcccctccga ggaggatggg gacgtatcgg acctggcggg gtccatccgg gcccacctcg
     8281 aggagaacgg aaggtggctc ctcgtcttcg acggagcccc cttccctgag gccctggacg
     8341 gattcctccc ggagggagga ggcgacgtcg tcgtcacatc ccggatcccc ggatggcggg
     8401 ggtggtcggc cctgaaggta ggtcccctcg acctccagga ggcggccgac cttctcctca
     8461 aaaggacgga gagcgaggac gcctctggag cagcggcctt ggccgaggag ctggaccgtc
     8521 atcctctctc cttggagctg gcggcggcct acctccgctg gacgggagct tcgatctcca
     8581 ggtatctgga atccttgagg cgccggttcg ccggggcccc agccagaaag ccccacgact
     8641 ttcccgaacc cctagccgcc gtcctggaga tctcggtaga gcaggtcgga gccgtctccc
     8701 aggaggggtc agacctcctc accctctcgg ccttcctcgc ccccgaggat ctccccgtcg
     8761 accttctcga aaagaccgcc gccctcttgc ccgagtcggt ggagccgggc cttagggccc
     8821 tcgagcggag gggccttgta aggcggggcg aagagctcct ttctgttcac cccctcgtcc
     8881 aggccttcgc cttcagtcgt ctcgacgagg cggagaggaa ggactgggcc gccgagacgg
     8941 tgcgagccgt cgggggtgcc ttcagctcct acctcgagga cctatccacc tggccagaat
     9001 gccgccgcct cctcccctcc gccctcttct ccaccaggag ggtcgaggag gtgggaggcg
     9061 gctccgagga ggcctccctc ctcctgagcc aagtaggcct ctacctccac cggagggggg
     9121 acctccgggg gtcgaagggg gtccttgaga cgctgatcgt catcgacgag aggctccacg
     9181 gccccgacca tccggagttg gccaccgacc tcaacaacct ggggtcggtc ctccgggccc
     9241 tcggggacct tccgggggcg aggaggtcct tcgagagggc tatcgccatc gaggaatctc
     9301 agccgacgcc ggacaggctg aagatagcca tcagggagaa caacctgggg acggtcctcc
     9361 gggccctcgg cgaccttccg gccgccgtcg cctccttcga gagggccctc gccatcgacc
     9421 gggaggccta cggcccaaac cacttcaaga ccgccatccg cctgaacaac ctgggggagg
     9481 ttctccagga gatgggcgac ctggaggggg cccgggacag ctaccagcgg gcctatcggg
     9541 tcttcagcca gatcctgggc cccggccacc actacacccg tcgggcggag gagaacctcg
     9601 cctccctccg agaggaaggc tcaggctgaa gaagaaaaag gggtcgagga tcgtggactt
     9661 acggtgcttc aggccccttt cgcctggcgc tcagagccga tcgggtatct tccagtccgt
     9721 gggatcgaac tcgttgtctt tgaagtcctt caccacggcg tggcctgaag ccaccagcat
     9781 ggcgttgaag ttgacgagct cgcccgtctc ggggtcctcg acgtaggcga ggcagatcca
     9841 tcggccgtat ctatctcttc ctcggaggtc gtcgacgtcg agataaaccg tctcgccggt
     9901 gaggatcgtc tcggcgaaga acttggaggc cttccccgcc ggggtgtcca tctcgggggc
     9961 gtcgacgtcg gcgagcctca cccggccgaa gccctcgacg tcgaaggtat ctccgtctac
    10021 gaccttcgtc acgagccccg agacctccgg cgaaggtccc tccgaacctc ctcccagggg
    10081 gaagaggtcc cctagggggc tatcgtccag acagccccct cctgcgagga gggccgagat
    10141 gacgatggcc atcggcagta tgtatgggat cttcatccgg ggctcctaat tggtggcgac
    10201 ggtccgacga gctcgggtcg gtggctgctc atgggaataa aagagatggc cggctaccgg
    10261 cctactctcc cagctcctcg atgatctcct ccacctcttc ccgcccctcc tcgtacttct
    10321 cctcctcatc gggggcgagc tcctgcagga gcctcagaaa ctctccggcg tggacccgct
    10381 cctcgtcggc tatatcgacg aggacggctt tagccagctc gtcgtccgta gactccgcaa
    10441 gctgcatgta gagctggacc gcctcgtact cggcggcgat catgtacctg atcgcccgga
    10501 tcagttcctc gagggtgagc tttcggtcgc aggcgaggcc tgaaaagggg ttggcaaact
    10561 ctggcatctc ctatctcctc ctgaagactt tttttcagca agtttaaccc tatcgcggcc
    10621 aagcgataag agtttcggtc ctcccggatt tactgagcct gcggctcatc ctcctcccgc
    10681 ccttcgggcc gccgccaccg ccaggtagat gaagggggtg tccagggcgg cgatgaggag
    10741 ctttatggcg tactgcccca ccatcatctg gatcaggacc gagtttggaa ggatgccgta
    10801 gaaggcgagg gtgatgaaga tgagggtgtc gatcgcctgg gaggcggccg ttgaggcgtt
    10861 gttcctgagc cagagcctct tccccccggt cttctccttc cagaagtggt aggcccagac
    10921 gtcgtgatgc tggcttacca ggtacgccac catggaggcg gccacgaccc tgggggcgaa
    10981 gccgtagacg gcgtcgaagg aggaggcgag gcccgggtcc atcagcggcg atgccgacca
    11041 ccggagggcg atgaggacgg agacgaacat caccacgttg gcggctagac ccgcccagac
    11101 cgcaagcctc gccttctcct tcccgaagag ctcgctcagg atgtcggtca aaaggaaggt
    11161 ggaggcgtag acgaggaccg ccgnngccgg agcgatgaag gggccgaagg agatggtctt
    11221 gacggcgaga aggttggcga ggaccgtcag ggacgcgaag accccgacgg cgatctcagg
    11281 tccccggcgg gctgcgagga tcgccgaggc ggtgacgatg gcgagggtcg ccgccgccca
    11341 gaagatcggg acgaggtcca tctcgcccct cagccctcgg tgccggcgag ggcgaagtag
    11401 gcgaggagcg cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctgtccat cagcctcacc
    11461 agggtcggtc cggagagctc gtgggagtcg aggaggctga gggtctgccg gtggatctga
    11521 atgatgacgt cctctccgga gagcccctgg acgaggagga ggt
//
LOCUS       DASM01000151            3581 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_80663, whole genome shotgun sequence.
ACCESSION   DASM01000151 DASM01000000
VERSION     DASM01000151.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_80663"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          746 a         1111 c         1080 g          642 t
ORIGIN      
        1 tcgtccagat gaacgtcgag gacacccgcc tcgggaccgc cgagtacgtc tcgatgaggc
       61 acgagctgga caccctggag ctgaagtggg ggcagggggc aaagtgcatc gggggcgaga
      121 tcaaggtccg gtccctggag agggccctgg agcttcagaa gaggggctac atcgtgaggc
      181 ccgacccctc caagagggag gtccagcagg ccttccggga cggggcgata aaggagttcg
      241 agcggcactc ccgcctcggc ttcgtcacca aagaaagctt cctcgaagag gtggaccggc
      301 tcagggacct gggcttcaag aggatcaccc tcaagaccgg ggcctactcc atggcnncga
      361 gtgggggatc cccaccttct acatccagtc cctcgcccgg gagttcgcag agcggctcgg
      421 aaagaggggt atgagggtcc ccgacctcgc catcgccggc ggcttctcct ccgaggacgg
      481 ggtcttcaag gcgatcgcca tcggcgcccc ctacttcagg gcggtctgca tgggcagggc
      541 gctgatgatc cccgggatgg tcggaaagaa catccagaag tggatcgggg ctggagagct
      601 ccccaagtcc gtctccaagt acgggacgac cgcaaagcag ctcttcgtca cctacgagga
      661 gctggccgag aagtacggaa gggaggtcga ggagataccc ctgggggcga tggggatcta
      721 cacctatgcc cagaggttcc aggtcggcct ccagcagctt atggccggga gcaggaactt
      781 cagcctcaag accatatcga gaaacgacgt catggccctc accgaagagg cggcccgggg
      841 cagtggcata ccctacgtca tggacgccta tcgggacgtc gccgaggaga tcctcgacgg
      901 ctgaggcgtc cctatttggc ggatgagggg atggggccgg gatcggctcg acggccgagc
      961 cgccgaagat cccctccccc tgccaaatac aaatttatta ataaagtttt atctctttta
     1021 ttctcgtccc tcctccgccc cgccatccag aatgcttaaa tattaattcc cggccaggat
     1081 ggtccattta gtcataaata aatgttactc ataagattta actatgggtt gcagacatgg
     1141 ccttcctcct aactcgttgt gatcgatggt ttggcggaag ccaagtaggg tgatataggg
     1201 atggcagaac tcaacccatt tgagatagct aaagagcagc tagataactg tgcaaaaata
     1261 atgaatctga gtgagggcgt ccgcgagatg ttgaagcacc ccatgcggga gcttcacgtc
     1321 tccatcccgg tccggatgga cgatggaagc accaaggtct tccaggggtt ccgggtccag
     1381 tacaacgacg ccaaggggcc ggcgaagggc ggcatcaggt tccaccccga tgagacgatc
     1441 gacaccgtca gggccctcgc cgcctggatg acctggaagt gctccctcct cgacctcccc
     1501 ctggggggtg ggaagggcgg cgtcatctgc aaccccaagg agctcagcat gaccgagctg
     1561 gagcggctca gcagggggta catcgaccgg atctggcagt tcataggccc cgagaaggac
     1621 gtccccgccc ccgacgtcta caccaccccc cagatcatgg cctggatgat ggacgagtac
     1681 tcgaagatca cggggaagaa tgagttcggc tgcatcaccg gaaagcccct ctgcataggc
     1741 ggctcctgcg gccgagacga cgccacagcc cggggcggca tgttcaccat ccgggaggcg
     1801 gcgaaggagc tggggatcga cctctccggg gctacagtcg ccatccaggg gtacgggaac
     1861 gccggatgcc atgcctcctt cctctgccgg gagctcttcg gctccaaggt ggtggcgatc
     1921 agcgactcca agggcgggat ctacaacaag gacggcctcg acctggaact cgcctgcagc
     1981 gtcaagaaag agacctgcac cgtcgtcaat tccccgagga agacgacggt caagatcacc
     2041 aacgaggagc tcctggagct cgacgtcgac atcctgatcg tcgccgccct cgagaacgtc
     2101 atcaccaagg agaacgcccc caggattaag gcgaagatcg tcgcggagct cgccaacggc
     2161 cccaccaccc ccgaggcgga cgagatcctc tataagaagg gggtccacgt catccccgac
     2221 ttcctctgca acgctggcgg cgtcaccgtc tcctacttcg agatggtcca gaacatcgac
     2281 aggtactgct gggacgtcga agaggtccga aagaggctcg acgccaggat gacccgggcc
     2341 taccactccg tcctcgagac ctcccgggct tacaacataa acatgcgcca ggcagcctac
     2401 gtcgtcgccg tcggccgcgt cgtcgaggcg atgaagatcc ggggctggat cgggaccgac
     2461 tagccatctc tcttctcacc agccagagga gaaaaaacgg gaaggctcgg gagatgatcg
     2521 ccggggaggg tccgatcctc ccaatttctc atcttttttc cgtctcgctt cgccaccttc
     2581 gcgccggact ggccggagga attggcgttc ctgatgggga tgatatatca ttttcccctc
     2641 aagttccgcc gccggggtcg aatttttcca tcagatggag aactgatata ttgatggaac
     2701 ggccacgcct aataattgtt aaagttggtg acggaggaac gtaagatgaa actgaacctt
     2761 accgccgaga tatgggaggg ggaccggtct tacatctcaa aatgcccgga gctggggatc
     2821 tcaagcatag gcgccacccc cgaggaggcc ctcggccggc tggaggaggc ggcctggttc
     2881 tacctcgagg atgccgagag ccggggggtc ctcggcgacg tggagtcggc tgcgatctcc
     2941 ccccacaagt tcgccacgag gatagagctg gtcagatgag cgatctgact agtaatattt
     3001 cgtctcgaat tacattatga aacggcctag ctggagcagg cagccttctt cttattcttc
     3061 tcctcggacc tccccagggt ggggttcaga gactggggcg ccagctccac cagcctgtgc
     3121 cagttatccc tctcccagag catgtgagac cggtcctcct cggggatgaa ctggcggagc
     3181 cggagggcga gggtctccca gttgacctgg tggccgtcga agtccgggcc gtcgacgcag
     3241 gcgaacttcg tctctccccc cacctcgacc cggcaggcgc cgcacatccc ggtcccgtcc
     3301 accatcaggg gggtgaggct gacgatcgtc ttgaccccga agggctcgct cgccctcgcc
     3361 gcctccatca tcatgaaggg gcatccgtgg acgaagatac ggtcgatctt ctcctccgca
     3421 agcctccgct tgatgaagtc gttcgtccat ccggcatcgc cacagcttcc gtcccccgcc
     3481 aggatcacga cctcgtcgct agcctccctc agcctatcct cccagaagat gaactcccgg
     3541 ctccgcccct ccaccgccgt tatcacccgg tttcccgcct c
//
LOCUS       DASM01000152           36365 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_16824, whole genome shotgun sequence.
ACCESSION   DASM01000152 DASM01000000
VERSION     DASM01000152.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 36365)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 36365)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..36365
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_16824"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         7257 a        11110 c        10916 g         7082 t
ORIGIN      
        1 tcgtttgcta cggcatctac gatagtagtg gtgttgattg ccccatattt gttgatcaga
       61 tcgtctcttt tggccttggt gaggagtatc ccggccacat tgccgacgag cctctcctct
      121 accagctccg ctttaacccc ctgggagatg atcatctcgc cccagagctg attggacggc
      181 gtataggcat ccgggcgata cttcccggag gtgatgtaat cggcggcctc tccggagggg
      241 attgctctga gccttacaga taccgggttg ccatcgatct ttattcccgc atcgttgaag
      301 tcttttgcca cctccgtcag ccagccgtcg gtgccgctcc cggctttctc cgtcgaggag
      361 aatatctcga tatagctgga tgtggtctct ctaacctgag gcggataact gtcgtctata
      421 tcgggcaagg tatcgttcag attttcggga ataatgtcga ccggcatcgg ctcatgggtg
      481 actctattaa cattaatgtc ttcgatgatg gactccagcc tttcgactgg atcgcgttca
      541 tctggacctg gaccaacgat tagacctatc cccagatata ccaagataaa tactcctact
      601 gttgccccca atatcttcca aattactttt ctgtcggcat ctttgagcat gctgattttt
      661 tcctcctcaa ttcttatagt ttgttgtctg cttgatcagg ttatcgatct cgaccatccc
      721 gggcatcttc tccagctggc cgctctccac gctgttcagc cccgagagct ccagaaggag
      781 cttgtcaagc ttcagcagga tcgcctcatt atcttcggcg gcattcttta caaaagtaat
      841 atatttatta tagacttcta tcttttcatc catgaactgc tgggaaaagt ctccagaatt
      901 gcttttgttt ctgatgaaat tatagtcttc ttcatcaaaa gcatccaatt tattgataat
      961 gctgtgcata ttcataaaga atatcctctc cacctcggag atgacgccgt caaatttctg
     1021 gtagctgatc tcagaggagc tgaagatctg cagaaggatc tcccggatta tcttattctt
     1081 cttctgcagc cgatcgatct gatctagcat caggtagatg tttttttcga atgttttcag
     1141 ctctatccgg cctttcagcg catcaatata atcctccgcc gtcatgatct tttgagtcgg
     1201 agtctccctc tctggctcgg cgaggagcct gtagttcccg tagatcaacc cggcgccgct
     1261 caagaagatg aaggcgcagc caaaggccgt tggcagtgca ccgaccaaga cgaagacctc
     1321 cggcaagacg attatgttgg ctgcggcgat ccccagattc aggcccagga gtttgatctg
     1381 cttctctctt tccatctgct attgccctca catttgctct gcccggctcc tcggagaggc
     1441 tggaggtcga acccctatcc tcctttctga agcgtgtcgg agatacgaag ccaggagaac
     1501 ccggtcatct tccctccagc gcttttttag tgtacgtcct aggatttagg acttacgcac
     1561 ttggcagtac cttcttatgt ttcgcgaccc taatttagat gaacaagtcg aattagtcga
     1621 cgcaccatga ggcagatctg cggaggcatt gattaataat aaattttatt agttgtatca
     1681 ggaacgttat tgataaattt atttagctgt tcgaaattct gagagcgacg aagatggata
     1741 aaccgagaaa agatttgaac tattgatgaa aacgaaggtt gcgcttccag gcttttcgga
     1801 tcttggatga tcttctggag agctaaatcc tccagtacgc cgaggctcag aagatactcc
     1861 cggtcaccat cccctgaagg ggcatagcca gccctcatgg ggcaggagtt cttctatatt
     1921 ccaggatata accctagcta ggattcgatt tcgaaaatca attacgcaaa gtcaaaatat
     1981 tagggaccac gcatttccat gcaaaagttt atttatacat gataacagca taccatcgga
     2041 ggtttaaata cgcgtagaag ggattttgtc agtgcattga ctgtttttat tatattgttc
     2101 ctttccggga cgacgtttgc tgcgtcggaa gaagaatatg aaggattcga gacgaaaacg
     2161 cctctactgt ccgctgagtc cgatgaccaa gccgatggcg ccctgagcag atctggcatc
     2221 gatcagctga cgaacagaag aaaggttctg ggcagggacg ccgacctgga atcggtggcc
     2281 gaagaggacg tcgtcgcacc ccggggggag ccccgcctcg tggtcatggc tgccggtgcc
     2341 accgtccgag aggatgaggg cggccaagag ggcaccggaa ccgccgcaga tctcaaccag
     2401 gcagaatcgg gttcaatttc gacctccggc tgcgatccgg tcatcggcat ctcctatcgg
     2461 gactcgaccc cctgcaccgc ggaagtggcg cagccaccaa aaggagctcc caacgtcgtc
     2521 ttcatcgtcc tggacgacgt gggattcggc cagatagggt gctttggcgg ccccatcgat
     2581 acccccagca tcgatcggct ggcggctggt ggacttcgat acaacaactt ccataccacg
     2641 gcgatgtgct ctccgacgag ggcctgcctc ttgacgggga gaaaccacca ctctgtcgga
     2701 gtaggggcgg tcatcgagct gcccgcgggc taccccggct acagcatgac cctctcgaag
     2761 aacgcggcca cattgccgga gatcctgctt gaaaacggct tcaacacctt cgccgtggga
     2821 aaatggcacc tctcagatca cctcaacgca gcaggcccct tcgataactg gccgacggtc
     2881 aggggctttg aaaaatacta cgggttcatc gggtctgaga ccaaccagtg gtatcctgat
     2941 ctggtcagtg gcaccaccag ggtcgatcct cccaaaaccc ccgaggaggg gtatcacatc
     3001 tccgaggacc tggtggaccg ggccataggg tttataaaga gtcagcaggg cgtagagccg
     3061 ggccatccct acttcctcta cctatccttc ggagcctgcc atgctcccca tcacgctccc
     3121 aaagagcaga tagatatgta caagggtagg ttcgatcagg gctgggatga ggtcaggaac
     3181 gaaaccctgg cgcggcagaa ggcgatggga attgtccccg aagatacgga gcttccgccg
     3241 agaaacccgg atatccccgc ctgggtggag ctgagcgaag atcagcagag ggtctttgcg
     3301 agggagcagg aggtctacgc cgggtttttg acccatacag acgcccagat cgggaggttt
     3361 ttggaccacc tcgaagatat gggtcagctg aacgacacct tgatcgtcct catatcggac
     3421 aacggcgcca gccgggaggg aggattcaac ggtacctcga acaactacct ctacttcaac
     3481 cgcatcattg agaacgtgac ctccatcctc gcccatatcg acgagctcgg gggaacggag
     3541 acctggaacc actacgcaga gggatgggcg atggccggga acaccccctt caaaaggtac
     3601 aagcagaaca cccatgaggg agggatccac gaccccatga tcatctacta cccggcgctg
     3661 atagaggacg ggggtgggat aagggagcag tacacccacg ccgtcgacat agtccccacg
     3721 gtcctggagc tcctgggcct ctcggcgccc gaggtctaca acggccatcc ccagaagccg
     3781 atcgaagggg tgagctttgc cgcaagcctc tcagacccgg aggcggagac gggcaagtac
     3841 atccagtact atgagatggt cggcaacaga gccctctggt acaacggctg gaaggccacc
     3901 gcttaccatc cgccagagtc cggcggcgac ttcatgaacg acacctggga gctttacaac
     3961 atcgaccagg acttctccga ggtccacgac ctggcctctg aatatcccca gaagctccag
     4021 gagatgatag acctctggtt ctttgaggcc ggaaagtacc atgtcctgcc tctggacgac
     4081 cgccagaagg ccaggttcca gccggcccca aaaaccggcg tcttcaccta ctatccggga
     4141 actgagaaga tcctcgagcc ggagctgccc gacacattga accactccta caggatcgcg
     4201 gcctacgtgg ccattcccga atctggcgcc gagggggttc tcttctccat aggcggccgt
     4261 ttcggcggcc tatccctatt cgtcatggat ggccatctcg tctgcgacta taatttcgtg
     4321 ggcatgagac attatactgt atcgtcgacc tctgaggttc cagccggtcg ctccgctcta
     4381 tcgatggcct tcgacctgac gggaagccac cagggcgtct gtaccctcta tatagacggc
     4441 caggccagag gcagctccga ggtgatgata cagccgtacc gctactcctt cgaggagggg
     4501 cttgagatcg gcaaggaccc ccagaccccg gtgagcgaga gctacgagag ccccttccgg
     4561 ttcacaggga ccatcgagaa ggtagtaatg gaggtcgagg gttaggagcg gtgcaagtag
     4621 acgatgcggg agatcatggg atgaatggta tgaaagaatg gctcgtcggg atcctgggtt
     4681 cggccaggat tccgatatat ctttttaaga tttcgaccgt cttttcagct tgatgagacg
     4741 gccctattct attcaatcca tccgatgttt gtctcaagaa aaaatagtta tataggaaaa
     4801 aaaacattta tccaaatcgg aggagtttgt atcaaaattg ggcttctaat caaattggct
     4861 atagcggcat tttttcttat ctcaattgcc gcaggcggcc tcgattctac gaacaccggg
     4921 ctgtccgtca tcccgttgga tctgaaagca ggcggcttcg gcaatagtgg gagaagcgac
     4981 gatcacgccg agtcgccaga agatatcgga gcagacgttg taacgaacag aaggaacgtt
     5041 ctctcaaata actccagcct tcatcaggcg atgcacgagg acgtggcccc ggccccaagg
     5101 ccgaacatcg tgatcatgct cgccgacaac atggggtaca tggaccccgg ctgctacggc
     5161 ggcggcgctg tattgggcgc tccgaccccc aggatggacc agatggcaaa agagggcctg
     5221 aggctgacat ccttctactc cgagacccag tgcaccccca ctagagcggc gatgctcacc
     5281 ggaagattgc ccatcaggac ggggatgtcc ctcgcctcgg agacggggtc caatgcgggg
     5341 atatctcctg aagagatgac cctggcaagg gtgctatccg atgccggcta taatacgggc
     5401 atgttcggca agtggcatct tggggacatc tacgagagcg agcctcagaa catgggattc
     5461 gacgagttct tcgggctatt atatcacctc aactcctata ctcagagcga gcggatcggc
     5521 ttcgatcctc gctgggaggc gggaaagccc ctgggactgg tggaggccaa gaaaggcgag
     5581 aacctcacag aagtggcgcc gttaatccca tattttgagc ctcactctaa tagatggaac
     5641 gtaacggcgc tctcaataat cgatgagcgc tgtgtcgaga gggcgatcga gtacatcgaa
     5701 aatcaatcaa cctccagcga gcccttcttc gcgtacatcc ccttcacccg gacccacttc
     5761 cccagcgttc aaaacccgag atgggcgggg aagtccagca aaggtccgta cggcgacggg
     5821 cttatggaga tggaccatca cgtcggcatg gttcttgaca cccttcgaga gctggggatc
     5881 gagaataaca ccatcgtcgt tctgaccagc gacaacggac cgaccctcga ccagtggccg
     5941 gactcgggct tctctccgtt ccggggaggg ataggtacca cttatgaggg aggcgtccgc
     6001 gtcccctgca tcttctggtg gcctggaacg atcgagggcg atagatcgtc caacgagatc
     6061 gtctgcaccc tggacctctt caccaccctg gccgtcctcg gcggcgggga gattcccgcc
     6121 gaccggccca tcgacggcat cgaccagtcg gagttcatcc tggggagaca ggagaactca
     6181 aaccgagaat gggtggtctg gtacatcgga actgggagtg cggccaacac catccccgcg
     6241 gccgtccgct ggcatcagtt caaggttcac accagagcgt atgactcctt ccaggggccg
     6301 gagagctatt acggccagat ccccgccgtc tacaacatcg agatggaccc ccgagaggag
     6361 cacaacatcg ccggagagca cgatttcgtc atcagcgcct tcaccaagat ctatcgagag
     6421 ctggtggcca gcatgatcga atatccgaat acgccttctc gggcgtatcc aatctcggcg
     6481 ggagcgagct gacggagacg aaatcgtctc cattctcttt tttttaatat gaacaataaa
     6541 tataacagat ttatattgtc aggcaatatg agtcctgttt taggagtttg aacgcacggg
     6601 ttcgatctga acgccatggc aaatacgctt tgaattgctg caaaaaatga gggacctgat
     6661 tcgcctggtc gtagctgcca aggcaaatca tttccaattc gcgagagggc ggcgatgtgc
     6721 gccctccgcg attagccgaa cttgatgacc ctatcgtagg cgatctcgga gtaagatggg
     6781 cccacattcg tcccattctc tacatatgcc ctgaaggcca cgaaagcggt ctcgttcagc
     6841 cctatgccat aggatccggg acccgtgggg acctctgtac agtttacttc gccattgcag
     6901 tcccgggcca tcttccaaac gtaaaggtat ttggctgccg gattatcggg gatgtactcc
     6961 actgcagttc cctcgaacct cctgttattg acagaggcaa cgccgttatc cagtttcgct
     7021 ccatagatgc ccaggttgga ataagtcgcc ttgcccgttg cttcgtgatt caccccatag
     7081 attataagga actggtcggg atcgtcgccc agcacaaagg gcgtggtgtt gaggtaggtc
     7141 gtatctcggg tgacgccgat gacgtccatc cccctctgca tggcgtcata gccttcgggg
     7201 agccagacac cggtgatgag ctccgtggcg ttctcctctc catacttctc aaggatctcc
     7261 gccctcagct cgaagagagc aggcatcaga tcgagctccg tggcgtcgcc attccccctc
     7321 accctcagct ccggcatcgg gaatggatcc ggctccacgg actggttggg agttatgcga
     7381 aggacaactc ctggggtctc atcgaggtaa tccgctcccg aagtctcatt ttggaagtag
     7441 gtcatcctct gaaggaagat gaatgcatca gagtatacat cgacgcccat cctcagaata
     7501 ttggatggca agatctcggt gttgaagata tcaggatcat aacccgagga agtggcagcg
     7561 gctttgactc tgttatcgat tcctctgtca gcggtgctca cgatcattat gtccttatcg
     7621 aaggaatcac cctccattcc cccgctggta ttggcggtca aaagatttat ggtgtctcca
     7681 acgttcccga atatcttctt gaacttgccc tcaatgggat agtaatggaa gtactggaag
     7741 gtctgatagc tgaagtaggt acagctgggc ggagtcttcc cgatgtaaac aatggcttca
     7801 tccggcccga gcctgtagcc tatggacagg ttcatcttat cttcgaaggg gttgggcgcg
     7861 atctgtcccg gcgcccgggg caggacgtaa gccttgtaga aggcgccggc gttgttgccg
     7921 ttacaggaga agactatgcc ctcattgacc aggtcgaaga cgttctgggt cttcagctct
     7981 cccagctgta cggtgaaccc atcctcctcc agagcttcaa tgaaggcgag gatgtcaccg
     8041 cctccaaggt cgcccctggc catgccatct tccggggatg tgccgttgag ccaggtgaag
     8101 ttcatagatt tcaccttcgc catatgctcc ttgaactgct ggaggctggc ttgtttctcg
     8161 tcggtcatcc ccgaggtcga ggtctgtgcc agacacaatc cggccaggat ggttgtggcc
     8221 agaaacatgg ccgcaagttt ttgacaatac gtcttgattt agatcccacc ttactttatc
     8281 gggtatagga gagcctacgt tattaacttt aggtattcag catatcgaag ttcgggcgga
     8341 taagaaggtt aattagctct gaacagagaa ctgcggtcga tgagcaagaa gggcccactt
     8401 cccggctttc atctcatggc caagcccaca ggggcgatct gcaatctcgg ctgcagatac
     8461 tgcttcttcc tctccaagaa ggacctctat cctaagagca gcttccggat gtccgacgag
     8521 cttctggaga gctatatccg ccagtacgtc gaggcccaga agaacccctc ggtcaccgtg
     8581 gcctggcagg ggggagagcc gaccctgatg ggcctggact ttttccggag gtccatcgag
     8641 ctcgaagaga agtatcagcg gcctggaatt acatttcaga acaccatgca gaccaacggc
     8701 accctcctgg acgacgaatg gtgcgagttc tttcgtgaga gtggctttct catcggcctc
     8761 agcctcgacg ggccgaggga gattcacgac gcctaccggg tggataaggc cggcgggccc
     8821 accttcgacc gggtgatgaa ggccgcccgc ctcctgcgga agcaccgggt cgagttcaac
     8881 atcctcgcca ccgtccacgc cgccaaccag gaccatcccc tggaggtcta ccgctttctc
     8941 cgagacgagg tgaagaccga cttcatccag ttcataccga tagtggagag ggacaacgag
     9001 acgggatttc aggaagggga ccgtgtcacc ggccgatcgg tggaccccga gaggtacggc
     9061 cgatttctca tctccatctt cgacgagtgg gtcagaagag acgtgggaaa gaccttcgtc
     9121 cagatcttcg atgtggccct ggccgcccgg tttgggacac catcggggat atgcgccttc
     9181 tcggagacct gcgggacggc gatggtccta gagcacaacg gcgacgtcta ctcctgcgac
     9241 cactttgtag agccgagata cctgctggga aacatcatgg agaggcccct cgcttcgatc
     9301 gccggctcag caaagcagcg aaggttcggc cgggagaagc tggacctact ccccgaatac
     9361 tgccggaggt gcgaggtgcg gttcgtctgc aacggcgaat gccccaagaa ccgcttcatc
     9421 aaaacgcccg atggcgagcc gggcctcaac tatctctgtg ccggctataa ggacttcttc
     9481 aggcacatcg acgggtcgat gcggttcatg gccagcgagc tgcggtgcgg ccgcagtcct
     9541 gccaacatca tgagctatac gggatcttcc gaaggaaagg tgatgagagc gttcctcagg
     9601 ccgggtcgaa atgatccctg cccctgcgga agcgggctga agttcaagaa gtgtcatggc
     9661 agagtcgacc atcaaaggga cgagcctccc gccgaggagg cgctgtcgaa gaagatctgg
     9721 gaccgttgca ggaagctctc cgacctgctt aagtgaagat tgcaggaagt tgtctcgcca
     9781 gcttgagggg aggtcaaaga tcttcatccg acctttccga ggcaggactc gtcgagatga
     9841 acgaattgct ggtcccttgc cgcctctgga tagctgccgc cagttgtacg ataatttaaa
     9901 tagaatgata tttcctaatt attatttgag atgatattat gcgccagatc gtcgttttct
     9961 tcctgatggc cctgatcttg atctctcccg tcgctttcgc ttcgatcgag gacgatgata
    10021 tcgcaagagc cgaagagttc ctgcccgcta agatcatccc ggccctctac aacatcagcg
    10081 tcgacccgaa ctggatcgat ggaaccgatt ccttctggta cctcaggacg ggtcgggatg
    10141 ggaaggagtt cgttctggtg gacgcaaaga atgggacgaa gcagcctgcg ttcgatcacg
    10201 ccgccctagc agaagccctg gccgatgcct cgggatctga ggtcgacccg tcggagctgc
    10261 ccttctcgga gattcagata gatgatctca ccatcaggtt ctcggccttc aacaggagct
    10321 gggagtggga ccgggaatgg tccgcgatcg gagaggctcc tcccggcaaa gaggcgggcc
    10381 ccggcgagat cctctccccc gacggggagc tcgccctctt tgtcgaggac ggaaacctct
    10441 gggtcaggaa gaccgccacc ggcgatagac gccccctgaa cgcagacggc tccgccaact
    10501 atgcttatgg caagcgatcg gatacggtat cccatcccgt cagtcaattg aggctgaacg
    10561 agaccgagaa gccctacgcc gtctggtccc ccgactcacg tcgcatcgcc accttcagga
    10621 tggatcagag gaacgtgacc gccctccacc tcctccagta cgcgcccggg aacggctcca
    10681 ggccccgggt ctgggaatac aggtttgcca tgcccgatga cgagacggtg cccatctacg
    10741 aacccctggt cttcgacctt caagagggga gggcggtccc cgtcgactac cgcccccagc
    10801 ccgaggtatc gctgatggat accgaggacg acgttctcca gatgtggagc gaggacggcc
    10861 agatcctcta ctccctgtac gccgagaggg gcgagaaggc cctccgcctc ctgaagagca
    10921 gcccggagac cggcgagacc gaggagatcc tggaagaaga gggacccacc tttgtggagg
    10981 cgaacctcga ttacgccagc cgccccaacg ttttggttct tgaaagcggt gatctgatct
    11041 ggttctccga gaagagcggg tatggccacc tatatctgta cggcgaggat ggagaggaga
    11101 agaacgccat aaccgccggg gattgggcgg ttcgaaccct cctccgcgtg gatgaaaata
    11161 attcgcaggt ctacttcacc gccggaggga gagagccggg gcgcgacccc tactaccgcc
    11221 acctctacag ggcgaggctg gacggaacgg atatagagct tctaaccctt gaggatgcag
    11281 accacgacct ggagatcgca ccgggaggga cggcatttct ggacgcctac tcccgggcgg
    11341 accatccgcc agttacggtt ctcagggacc tgaatggatc tgtcaccctg aagctggagg
    11401 agggggatat aagaaacctc accgatatgg gctggaagcc cccccagctc ttcagggtga
    11461 agtcggcgga cggcgatgct gatctatacg gcctgatcat caagcccacc agcttcaacg
    11521 aatcgttgaa gtaccctgtg atcgagaccg tctatcccgg accctggacg atcgtcaccg
    11581 ccaagtcgtt tcccggggag gagacctggg taaacaagat cttctggagg ggtcaggccc
    11641 tcgccgagct gggcttcgtc gtcgtcaccc tggacggtcc ggggaccccc tatcggtcca
    11701 aggagtttca cgatgccgga tatggtcatc ttggggattc gggagggctc gccgaccacg
    11761 tccaagccct ccagcttctg gcagaagata ggccctacat ggatctggac cgggtgggga
    11821 tcttcggcca ctctgcggga ggtttcatga ccgctcaggc tctcctcacc tatcctgatt
    11881 tctacagggt gggggtcgcc tcggccgggg attacgacag ccggttctat gccgccttct
    11941 ggggcgagaa gtacgagggg ctgaagcccg actacgatga gcagatcacc gccctcaagg
    12001 ccgagaacct taagggcgac ctcctcctgg tgaccggaga cgtggacgac aacgtcaacc
    12061 cctgcatgac tatgcagctc gtcgacgccc tcatcgccgc cgacaggccc ttcgaccttc
    12121 tggtgatgac caacaggaac cacgacctca gctacaaccc gtattacatc cgccgcctct
    12181 tcggctattt caaggagcat cttcgggggg cctcccaggc ccccgcctaa tttttttgtc
    12241 gattgaagag ccggaaagac ttttctcctg ccactgatcg agaggctgct tttctatccc
    12301 aggtccatta caattagctt aaataatttc ccaggcaaat atgatctatc ttggcccgat
    12361 gagaggccat cattgtggag tgggtttaga tgtctgagat tgacgaattg agatctctca
    12421 ccgccggaag atctcccaaa agaggccttc agcggaattt cgccaccaaa ttcggaccgt
    12481 tatcggtggc cattttcctc ttctccatga tcatcatctc atccgcccag ctcggatatg
    12541 agcccggcga gagggaggcg gagcggctct tcgagcttgg cctgggcggc ccctggataa
    12601 taagcgagag gcaccccgag atgtatcccc cggagaacaa gaccatgcct cctgccccgg
    12661 agttatcctg gtactcggat tacttcagca tcgtctcctt cggcggcaga gaggtggagc
    12721 ggtactacgt gacggggacc cccgtgattt acggtgccgg ccccgggggc cgtcccatcg
    12781 ccctcctcca gcccccggcc gccgccggcc tctggattct ggggccgagc agctggacgg
    12841 agtacgccat cgcgccgaag ggggcttatc tgaggctcat cgccctccat ccggccggag
    12901 gcgccgccga cctctacgag ataaccccct cctcgagggc ggttcagagg aggtacaccc
    12961 tctatccggg atacagcctc atcgccttcc atcctgaaga ggtgggaagg cacatcctcc
    13021 tcttctccgc cgacggcgtt acgagcaacg tggtggtggt ggacgtgagg caggcggcct
    13081 ggcccggacc tggacctggg ccccagcccg gctacggctc ctcccaggtg accctcaggt
    13141 ccactaacct caggggctac agcgtctacg tcgacggtgc ctacgtcgga tccgacggcc
    13201 aggtggggga cgccaaggac gggatattta cgatcaccgt ctcgggagga aggagccata
    13261 cgatcaccgt ctacggctcg gggcgcacct gcagatgtga ttattactac gagagcggta
    13321 ggagctatga gatcgactta tgctcgtgaa gtttaaggga cccggccggg aaggcgaggc
    13381 tgcaccaact caccggcgac gactcctgat tccgaccggt ggcgaccgat ggcggcgggc
    13441 tcggcgcctc gcccctcccg ccccctaagg agctccggac gaaaaaatgg ctgtccgatc
    13501 aatcgccctt cttccctcga acgacgacga aggagccgcg cccccaccca ggccgagggg
    13561 cctccacctc cgtcatcgcc tccggatcgc aggatagggt ctggacgaag gcgaggtcgg
    13621 taaatctggc atcttcgagg agggcggcca tctcctcagc ggcgagaaag cgggcctcac
    13681 cataaaagcc gcttccgcca tcgctttcac cttctctctc tcctttctcc cgctggtacc
    13741 gcctgccgac ggggctctcc cggtcgatga acccgacgac gactgccccg cccggcgcca
    13801 ggacccttgc cgcctccccg aaggcagccg ctctgtcgct gaggaggaag tcaacggtga
    13861 ccatcaggac caggtcgaag gccccgtccc ggaaaggaag ccgctcggcg acgccctgga
    13921 cgcaaccgac gccccgcctt cgggctgcct ctatcatcgc caccgacggg tccaggccga
    13981 aggtgactcc gaggggggcg gagaacctcc ccgttcccac ccccacctcc aggcctcgat
    14041 cgaaccgggg caaggaggac ttcacggcgg cgagctccga gaggtaggca gcccgatttt
    14101 tttcaaacca gcggtcgtag cgttcgacct ggtcctcgta ggggtcggat ctcggaattt
    14161 gcaatcacat cctaagggta ttgaaggaga tgcaacctcg gatcttccag ttcccagtca
    14221 tctattaacc ggatttacca gaattaaccg gatttaccag acctcaaaca ccctagaaaa
    14281 agtatgggcg agccttgggg cttgcccgcc ctgaatcgtc tcgactcaga tcttctccgt
    14341 cctgctcgtc ctcctctccc gggcgagctg atcgtccttt ccgagggggt agccgccgcg
    14401 gctgtttctc atcagcgcca ccgaacggct ggcgtcgcag ctcttgtccc gggagcggaa
    14461 gagctccatc gccgccgatg cgatgacggg gatggggacc tccttctcca gggcgtcgtg
    14521 gacgagccag ttaacctcgc cggtatcctc gacgtgggtc gggacgtcgc tgaacctctg
    14581 ctcggcgagg ctcctcgcca tcagctccac gagccagccc cggacggtgg cccccctcgc
    14641 ccagcccttg aagatcttct ccaggtctag gtggaagtcg ctcccccgga ggagagagac
    14701 cccctcgccg atcgcctgga gcatcgccgc ctccacgccg tcctggacga gcttggtgaa
    14761 gtggccggag ccgggatcgc cggtgtagat gcaggctccc tcggctgcca gactcgtcag
    14821 gatcggctct gcgaccttga agccgtcctt atttccgccg accatgaagc aggggccctt
    14881 ccgggctccg tccaccccgc cgctcgtccc gcagtcgagg aagaatatgc cgctgtcggc
    14941 gaggtcctcc tccctccgga cggagtcgag gtagaaggag tttccgccgt cgatcaccac
    15001 gtccccgggg tccaggaggg ggactagctc ttcaatgacc ttgtccaccg tcggccccgc
    15061 ggggatggat atgtatatca cccggggctg atcgaggaag tcgacgatgg gaccgtaccc
    15121 gtccaccacc ttgacgccct ttctctcaag atgaggcttt gcggacctcg acttccccac
    15181 cacggcgatc cccttctcct tcgccagcag tgcgaggttt ccgcccatct ttcccagtcc
    15241 gaggatgccc agcttcatat cctttggttt gctcaactga atcaccacca aatatcagat
    15301 tctctacgac cctagcgcga agactccagg cgttctatca ggctgttcaa ccgctccgtc
    15361 gaggagcggt aatcagagca atttgcctca tctcttctcc gatcggcgtc atccgaccgg
    15421 atccatcaat tgtatccatc ctgccagctc ggatccgagg gaggggaaga tggcgtcgag
    15481 ccgtcgagcc gccgacccgc tgcagccccg ggccttaaac tgcagatcag tccgccgagg
    15541 cgaccggccc gagcttggcg ggcctcatcc ccaggtcccg gtcgaggata tacaggaacc
    15601 cgctcccccc ttcaccgctc gccatcctca ccttctcgac gatctcccta gcgttcgcct
    15661 cctcctccac ttgctcctcg acgaaccact ggagcatatt ggaggtggct tgatccttct
    15721 cgccggccgc aacttcgacg agggcatgga tcagccccgt caccttcttc tcgtggcgaa
    15781 agacctcttc aacgacctcg aggggggagg cccactcgaa ggggggcttc tcgatggcga
    15841 ggagctccgc ccttccgccc cgggcgatga ggaagtcgta gatcttcatc ccgtgcatca
    15901 gctcctcctg ggcctggacc ctcatccagt tggcgaaccc cgggaggttc gccgccgata
    15961 agtgggcgga catggccagg tagaggtagg aggagtagag ctccgccctc acctgctcgt
    16021 tgagggctcg ggtcatcttc tcgccgatgg cagccatttt tcatcagctc cctattcagc
    16081 tctcctcaaa gacgtccttg gtcgccccgc agaccggaca ggtccagtcg tcggggagat
    16141 cctcaaaagc ggtacctggg gaaaccccgg aatctgggtc gcccttctct ggatcgtaga
    16201 tgtatccgca gaccgtgcag gtgtatctct tcgccatcca catcgcctcc tggaccggac
    16261 ttgtagaatc agtccgaggg cggccgggga tcccgaccgc caaaaacatg cctaataatg
    16321 ctacaatgta ttctccatcc cgaggtatat aatactttgg atccagggga ggggaacggc
    16381 cccctcccga gatcctccag cctcagatgt accctttcag aaccctcctg acctccccct
    16441 ccaggtcggg gatctttgag tttttatcct cgaagttgtc cgcgaaggtc ggagccgccc
    16501 gccgatggaa tacccccagg ggtatggggc catcggagtt gtagtcccac tcccgcatcc
    16561 tctcttgggc gaggacgggg tcctcggggt cgtgctcctc cagggcgtag gtccggcggt
    16621 tgtaaagatc gtacatgttg aagaaggtgg cacagacctg gaggacgtcg acgaaggcga
    16681 acccccggtg gaggatcgcc tccctcatcg tctctttgag gaggtcgatc ctgttcgagt
    16741 acccccgggc gatgaaggtc ccaccgctgg cgaagatcag ctccaggggg ttgatcggcg
    16801 aaacctccag ccccccgggg gtggacctgc ctgaaaagcc cgagggggat gtcggggtgt
    16861 attggccggt ggtgaggccg tagacccggt tgtcgtggac gatcgtcgta atctcgacgt
    16921 tcctcttggc ggcgaagatc aggtggtcga gcccctcggc gtaggcgtcg ccgtctccga
    16981 cgaagcagat gacgttgagg tcggggttag caagcttgat tccggtggcg acggggatcg
    17041 cccggccatg gatggcgtag aagctgttga ggtggaggta gtcggcgatc ttcccgtggc
    17101 agccaatccc ggtaaccacc acgacgttct ctattggtac gatccccgcc ccgtccagct
    17161 ctgccaatac gggctttatg gcgttcagga tggcgaagtt tccgcaaccc ggacaccagg
    17221 tattctgagc cgtcgttccc agatcctggg gcttcatgcc gccacctcca gaagcctctc
    17281 catcagaccc tccacggtga agggccttcc gtcatacttc aggatcagat cgtcggcctc
    17341 aaagccgtaa gaccggatca gccgggcgag ctgccccgtg gagttcgtct cgacggatat
    17401 agtcctctcg accccctgga gcgcctctct gaaccgatgg gcagggaagg ggctgacgac
    17461 caccggcgcg acgacccgga ggcctagctc ctccccgacc tcagaagaga cccctttatt
    17521 cgatccccag cataggatcg ccgtcgagga ccccgggtcc ccgtagacct tcaccgtctt
    17581 cagggaggcc agctcctcga cgagcctact ctccttctgg aggagcttat cctggcctct
    17641 ctcgatcgcc cgggcctcct ccgtcgtgat cccggagggg aggtgggcgt agctgttcgc
    17701 cttcaccacc gctccggccc gcggcgggaa ggccaggggg gagatcccgt cttctgggga
    17761 ggcgtacctc tggtaatcgc cctcgccgtc ccagaggacg ggggcgatat cggggatatc
    17821 cctcgcctcc tcgatattga agctgtaggc cgactcgctg agggtcttgt ccgatagcag
    17881 gaccacgggg atctgatacc tccaggcgag gcccagggcc gtcgccgacc agaagaaggc
    17941 ctcctccgcg tccccggggg cgatgacgag cctcggaaac tccccctgcc ccgcgctcat
    18001 gacgaagggg aggtcggcct gggcggtgta ggtggggact ccggtgctcg gccccgccct
    18061 ctgagagacg aggacgacga gggggatctc ggccatcccc gcgaggctca tcccctcatt
    18121 catgaggcag aagccgccgc cggaggtgcc gacggccgtc ttcttcccgg cgtaggcgaa
    18181 cccctcggcc atcaggatca cggcgatctc gttctcgggg tggacgacct tgatcgagaa
    18241 ctcccctgca accctggcga ggaagtggag gaccccggag gagggggtca tggggtaggc
    18301 gacgtaggcg tcgaggccgc cccggaggag gccgaggccg atgagatcat ttccggtgat
    18361 gacggggcag caggggtaag acctcctctc agccctacca tcgccccttt cctcgatccc
    18421 ggcgcgcccc cggcgggcga cctccaggtt ctcgtcgatg tacttgggga tctcctttcg
    18481 gaggaccttt tcaacgatct cctcctcgat cccggcggcc ccggagaggg cgccgatgag
    18541 gccgacgttc cttgcgatcg gaggcgcccc cgcctccctc acgaagctct tgagggggag
    18601 gcccgtcccc cccgccgggg gtgccttcac ctcgtcagag tcgtagatta cgacggctcc
    18661 ctcgttcagc cgccagaggt gccggtcgat ggtatcctgg ttcagggcga ggagatagtc
    18721 caccccgtcc cggtgagctc cgatcttctt tcccgcagcc cggacgatgg cgaagttgtg
    18781 cccccccctg atgagggagg ggtagtcgag gtactggtag aggcagtatc cgagctggtt
    18841 gtagaggcgg gcgatgacca tccccgcctc agctatcccg tcgcccgctc tcccccccac
    18901 cagaacggag atttcgtccc tcattgaaat ttctccttat tcagcggttc atcttctgat
    18961 gctatcgatg cccaagtggc cttttggccg attcatacct tcaaaagctt gttatagttt
    19021 aatcccgagg gtatataaca acttcgagca gcggctgttg aatcggagaa ggcttatatt
    19081 ctgccaaatg tcagaactcc gatgaaaggg aggaggctca ttggacctga gaaacgatga
    19141 aggctgcata ttctgcgata tcgtagccgg gagggcgaag gcccgccgga tattcgagga
    19201 cgacctgtcc ctcgcgatac tggacataaa ccccttctcc aggggccact ctcttgttat
    19261 ccccaagagg cacgtcccct ggtggcacga cctgacggag gaggaggtgg agagcctctt
    19321 tcgggtggcc aggaccgtat cccagaagat catccgggcc tatgaacccg acttcgtggc
    19381 gatatacgcc cggggtcgga ggatacccca cacccacatc ttcctcgttc cgacctacgg
    19441 aggcgacgtc ctcgacaggt tcttcaacgc cctggagggg ttccaggagg gggcggctcc
    19501 tatggcgagg ctgaaggagg acgaagagct cgaggaggcg gccgagctcc tcaggagggc
    19561 ctgagggggt cggcccctca aactactcct tgggaggtat cggccctccc accgacctgg
    19621 atacccagtc caggtagcca tcgtccccac cccttatcgg gagggcgatg atctcgggga
    19681 ggtcgtaggg gtggacctcc cggatcctgg aggcgagggg ctccaacttc tccgtcatag
    19741 tcttgatgat gaggaggtcc tccgcctctt ccttcacatc gccctcccag aggtaggtgg
    19801 accggacctg ggagatgttg acgcaggcgg cgagcccctc cccgacgata agccgggcga
    19861 gcctcccgga gctgttgggg ggggctgtgg tcaataccac gacgaaatcg cccttcaata
    19921 gctgccctcc cctgccgccg tgtagaaggc cataaccctc ctgcccaaat cttcggagac
    19981 cctctcggag aggagcctgg ccaggatctc ggcgtgctcg ggctggacgc agcagaggct
    20041 ctcggagccg tcgaggatca cctccgccaa gtaccggagc tcaaagaggt ccagcctctt
    20101 cagcctctct tcgaagtcgt cgtcgtcgag ggcgtagtgg tttgagactg gaggtaggat
    20161 cgactggtat tgaaaccctg aaccagagca ggtggtatcc gcgcactccg gggctagctt
    20221 gttgaggatt gagagatcct tatctgatag cgtcctggcc atatatcatc aaactcccat
    20281 ggatcagtcg gacctatgca gccagaggtt cggagacgag gacgaggccg tcctcggtga
    20341 tggccacctc caggtcccgg acggtgaccc tcttcttcaa cgcctccggc gccttctccc
    20401 tgacctggac gacgatctcg acggagccta tatcgagctt tatccccgcc ctgtccgcct
    20461 cccggaagac cagccccgga aggtcgaaga gatcagtccc ggcggggacg gtgagggtga
    20521 cgggggcctg gatcatcctc cagtccctca gggtggcccg ggcccgggag acgttctggt
    20581 gggcccgctt ctcgaggatg ctgacgttgg acctggtcgt ccccagaatc tccgccacct
    20641 cctgctgaga gaggccctgc cgcctcagct tcaggacagc cacctgcctt ctggtcagaa
    20701 ggccttcctt cgccgccgac ccctcctcaa tcccagcgcc ctcttcagcc ctttcggtca
    20761 tctatcgccc ccggtccaag atcttattcc gctccgaaag gctttaaagg tgccgaggta
    20821 gccaccccgg aatccggggt cttgcgtccc agccggtaca gacatgttcg tcataatttg
    20881 aaggtacccc accgaagggg ttcagggctt ccccccggag aggaagcccc tcgccctccg
    20941 gccgaggcgg cggagcccct cggcggcccc tcctgacggg ggcttgggac gtcgcggagt
    21001 cggcgccaat cccgcctcct cctccatcat cgagaggcgg gtccggtagg cggcgaggac
    21061 gaagccgtat agggccggcc ccacgatcac ccccataaca ccgatggcga atatcggcgc
    21121 cgcaaaggcg aggagggtaa ttgccggatg gatcgcagcc ccggccttgg cgagggcagg
    21181 ccgcatgatg ttctcgggaa atacgttcag aaataccgtc cccaggatca gaagagccgc
    21241 caccctaagg tagtcctgga agatcaggta gtagagggcc atggggacgt agacggcgct
    21301 cgtcccgatg aggggcatca gggcgaagac cgccgtgacc atcccccaga ggaaggggta
    21361 ggggacgccg atgaagaggt agacgaaggc ggcgatcagc cccgtgagga agctgtttat
    21421 caggtagacg ttgaagaggc tgttgtagat ggcgttcagc tccgtgagga aacggcgtac
    21481 catcgccctt tcgggcaata gacccagaaa cttctctatc gagcccgcgc cgtcgacgag
    21541 gaggtagtag gtgaggagga tggcgacgcc gaactgggcc atcaggatcg gcaggttctt
    21601 ggctatccag tcggcgaggc ccgtcaagat actcctggcc ctcggaagca cctccggtac
    21661 gagccaggtc ccgggagcgg ctaagaccgc cccaatctgc tcctccgcct ggtcgagggc
    21721 gtggcccaga tacggggcga cggcctgggg agatcgctcc accgcaaggc ccctcgtcca
    21781 gtcgaagaag tcccctgcgg tctggtctgc gaacttgctc gccgcctcgt cgaagtaatc
    21841 ctggttcttg gagagctcaa gcacctccgt cgtcagggcg ttgatgacgc tgaggacgaa
    21901 gacgaggaag atcaggaaga cgatggatat cgatagaagg gcagagaccc tccttcttct
    21961 ggtgagggcg tagaggcgga ggtggaaggg atggaggacg taggcgaaga agaagctcag
    22021 gaggatggtg gtgatgaagt tctgggtgac gtaaaagacc cccgccacag cggctatcag
    22081 aatcgccccc gccatccagt agaaccggtt catcgtggtc atgctctgag ctttttattg
    22141 gactgtgaaa tttatatgtc tttccttctc ttccgatccc cggtccgaga ggggagagag
    22201 aggcagccgg ccgtcttcgc ccggcaagac gatgatctcc gccgacttgg cccttagctg
    22261 aggcggcaat ggccgaatta tgctatatcc ccatatccat tccccggacc ccttccagga
    22321 tcgccccctc cagcttcgcg gacctgaggt cggctcgaga taggtcggcg ccggataggt
    22381 ccgctccgca gaggtcggcc cctacgaggg atgagcccct gagaactgca ccggagagct
    22441 tcgagcggac gagctttgcc ccggcgaggt ctgcccccgt gaggtccgcc ccccggagct
    22501 tcgccccccg gaggtcggcc tctatgagct ttgcaccccg gaggtcggcg ccggatagat
    22561 cggccctagt cagggtggcg ccccggaggt cggccccggc catggcaagg gagctgaggt
    22621 cggcgccgga gagctccgcc ccgacgagcc tcgcctcccg aaggtccgct cccgcgaggt
    22681 ccgcccccga gaacttggcc ccggagaggt cggctcctga caaatttgcg gatcggaggt
    22741 cggcggatac gagctttgac cttccgaggt cgacccccga gaggtccgcc cccctgaggg
    22801 aggcgcccat gaggtttgag ccctggaggt cggcgcccga taggttcata ccgctgaagt
    22861 cggcgccccg aaagtcagcc ctcatctgag ggtttgcggc ccgccaccgg ttccaggcct
    22921 cgggcccctg caggagggcc gagagctgtt ctggattcgc caagagatcc tcatcctccc
    22981 gtctttagtc ttcgccctat aagggcttga ggttaaaaag gagatacctc ggatgaggga
    23041 gagccgtcac catccagacc tcctccggac gaagcgggcc acctcctccg accccggggc
    23101 ctcgaagatc tcctgggagg agccgagggc gacgagggtt ccgcccatca ggacggcgac
    23161 cctgtcggcg agccgcctcg cctggtcgag gtcgtgggtc gccatcacca ccgctatctt
    23221 ccggaggacc gccccttcaa tcagctcctc gatcttctcc gccgtccggg ggtcgaggtt
    23281 agccgtcgcc tcgtccagga ggaggagctc cggctctgtg accagcgccc tcgccagggc
    23341 gacccgctgg gcctcccccc ccgagaggct ggagggcctc cggtcctcgt aacctgcgag
    23401 ccccacgtcc cggagggctc ccctcaccct atcccgggca gcggctccgg tgataccccg
    23461 catcgaaagg ccgtatgcga cgttcttctc gacagataga ttgaagatcg tcggcttctg
    23521 gaagaccatc gccatccgcc tcctggcagc agacctcgcc ccctcgtcct cggtcacgtc
    23581 gacgccatcg aataggaccc tacccccgtc gggggggtcg aggaggtcca ggatccgcag
    23641 gagggtagtc ttccccgctc cggtgggccc gatgacggcg aggacctccc cccggtcggc
    23701 cctcagggat acgcccttca ggatctccgc ccccccccgc atagccgtga cccccaccgc
    23761 ctcgatcatg cccaccagtt cccctggacc ctgttgacga ggaggttcac caggagggat
    23821 atggagacga ggatgatccc cagggccatg gagagctcga tctccccccg ggatgtctcc
    23881 agggcgatgg ccgtggtcag aaccctggtg tgaccccgga tgttgccgcc tatcatcatc
    23941 gccaccccca cctccgagag ggcccggccg aatccgagga ggaccgccgc catcatggcg
    24001 gccttcgcct ctcccatgat ggtgaggatc gcctgacgcc tcgaggcccc cagggataag
    24061 gccgtatccc ggacggcggg gcctacgccg ctgagggcgg cgatggtcag acctatcatg
    24121 attggggtta tgaggaccgt ctggcctact accatccccg cgggggtgaa gaggaggccg
    24181 aggctgccga aggggccgga ccgcgaaagg aggaggaaga cgaccaggcc gacggtcacc
    24241 gtcgggaggc tgtagagggt ctggatgagg ttgatgacgg ccctcttacc aaaaaaatta
    24301 tgaaaatgga tgaggccgcc gatggggacg gcgatggcgg cggcgatggc ggtcgaggtg
    24361 atggctatgg ccagggacct ggccgttatc tccactacgg ccggatcgag ggcgaggatc
    24421 agctccaccg ccttcgccag ccccgccgct atctcatcca atggcctcac ccgcgtgctt
    24481 tactcagtcc agggactcct tccgcgaata aggcggttac gctgaggccc tgctgcagac
    24541 gtcctcggcg aggggaacca ggcactcgtc cccggagcac cctatgagat cgcagttgcc
    24601 tctggcgggg ttgaagagag gctttccgat cgtctcctta ccgtactcgg cgatcaggtc
    24661 ctgcccctcg gtggagacga ggaagttgat gaagttcttt gccatctcgc agtttgtatc
    24721 ggggtagacg gcggggctga cgggtacggc ggcgtagacg ttgatcagga actcatcgct
    24781 ctcgatcagg ggtacgaggt cgagttctct cctgaaggcg gcgaaggtgg aggtgtcgca
    24841 gagggtgtag ccctccatct cgtcggccat catcagagtc gggcccatcc cctgggcagc
    24901 ctcaacgtac cagcgattat cggaggaggc tgcagaggct gcgatctctg cgtagtcgag
    24961 gccagctcta tcccagagga gcttctcccg ggcgtgggtc ccggagtcgt cgcctcgaga
    25021 tacgaacttg acctccccgg ggtactccat gccgtggagc atgatcgccc tcatcgcatc
    25081 ggaggcgttc agtccggtga tcccggcggg gtcgtcctcg gggcctacga tgatgaagta
    25141 gttgtaggcg aagcaggtcc gggagaggcc gtaaccatcc tcgatgaact ttctctccct
    25201 ctccacgtcg tggacgagga tcaggtcggc gtcgcccttg acggcccgct cgatggctat
    25261 ccccgtccct ccggagacgg tccggacgtc gacgttgtac atctcctcga agatgtcctc
    25321 catctcgtcc aggaggaggg tgtcgtagag gctggtggtg gtcgagatcc tcaaaagctc
    25381 ctttccgccc tcttccgccc cttcctcggc agccgctgcg gtgcagagga gggcgacgat
    25441 cagggcgaag gccgatattc gatgagatat taccttcatg atgatcaggg gtcgaatggg
    25501 gacgattact ataaaagctc tacgttaacg tctacggaat aagggctccg acccgcaagc
    25561 ccgacggatc gaccttactg gtcggtggtc ttggtggtcg gagcataagc tttagggata
    25621 tccccgacca tatcatattg agaaaagcta tggtggtgct ggatcgtgaa ggagtacaca
    25681 aacggcgaga tcaccgtctt ctgggacccg gagaggtgca tccatgcggc ggagtgcctc
    25741 agaggcctgc cgggggtctt caacccccag aagagcccct gggtggatat ggagggggcc
    25801 acctccgagg agatcatggc cgccatcgac cgatgcccct cgggggccct atcctacagg
    25861 agaaacgacg acctcggaag ggctaaaggc ccgggagggg ccccggccgc ggagatcagg
    25921 gtggtgaagg acggccccct cctggttaag gggaggtgct ccctcttcgg agaagatggc
    25981 cgactgatcg ccgaggaggg gcagttcgcc ctctgcaggt gcggtcgatc ggcgaagaag
    26041 cctctatgcg acggcagcca ccgggtctga tgggtcggag gcattggaga tcaattgggc
    26101 tgatacctcg tttttgcgat ttaagacaat aaagcttaac taagataaac ttctattgct
    26161 catattaagt tgaatttaat caatttaatt tgggtgaagg agttgaagca tcagaatgac
    26221 gtcctcggtg gagagagccc cagatcattc agcgattttc ctatcggcgc caatgtacca
    26281 taccggaaga tccatctttc tgggatagaa aaaggatgca gatcgatctt cccgatgagc
    26341 caaattttta tggatgatga catcggtgac tggaggtgct ccatggccta tgccgaaggc
    26401 ctcgtcttct tcggactgcc cgacttcgac gacttcggcg gaaccgttgc cctcgacgcc
    26461 gccaccggcg aagtggtctg gagccatccc ggcggcgggt cctggccggc cgtcgccgac
    26521 aggatggtct tcaccgtagg gaagggaagg gtctacgcct tcggcggggc gggagaagag
    26581 gaggatcacc agtgatccga cggctctccc tggccctgat gatgggcctc atcctcgcct
    26641 catcgatctc gccggccttc ggggaccatc tgaccttccg cggcgacgcc cagaggaccg
    26701 ggaacgtcag cggggacggc cccgccgccc ccctgctcct ctggaaggag aaagtgacgg
    26761 ccaagggcta cgttggaggg agcgcttcag tatctgggga gaggatctac gtctcctcct
    26821 ggccggagat gtcctaccag ggcgagcagg ccctcggctg cctcgacgcc agggacggat
    26881 cggtcctctg gcggaatccc ctggggggga aggggggagc ttcgaccccc gccgtctccc
    26941 gtggcctcgt ctttgccggc tcctggtacg gagatctata ctgtctggac gctgaaaccg
    27001 gcgagaccgt ctggaatcgg acggtcgaga gggaacccca atggtggggg gtagcttcgt
    27061 cgcccctggt gatcggggag ttggtatttg tctccacctt ctccgatggg accctccacg
    27121 tcctggacct agaaggtgat gagctctgga acatttcaac cggcaagatg gatccctata
    27181 cgtcccctgc cgccgaggag gggagagtct actttgcggg aggggacccc gccctctatt
    27241 gtttcgacac ctcctctttt gagatcctct ggagggctcc tacgaacgga cccataacca
    27301 cgacgccagg gatcggaggg gacagagcct acttcgccac gagggaggat ctccgcgctt
    27361 tggacgcctc caccggcgaa gaggtctggg tcgcgcccct caagggaacc acgtcgtctc
    27421 cggccatctc ctccggccgg gtctacatag ggaccgacga tggccgtctc gcgtgcctca
    27481 acgcctcgga cgggtccctc atctggagcg ccgaggtcga tagctccgtc aggtcctcgc
    27541 ccctcgtcgc gggagaccgg gtctatttcg gatctcaccg aggcgtcgtc tacgccctca
    27601 atcgatcgga cggctcggag atctggaggt acgagaccgg agcctatctg atgtcatcgc
    27661 cgtccttctc cgacggcgtc ctctacatcg gctcggacga cggctgcctc tacgccttcg
    27721 gggatgagac ctccaaggtc atcttcgagg gggaggtcct cctcggcgac gggagattca
    27781 acgctgcggc ggaggatggg agcgtccgcg cggtcgatga gaggacggcc ctgggggcgc
    27841 tgatgaaggc ctccgaggtg ggtgggttca acatgacctt cgacgactct gtggtggacg
    27901 attacgggct tttggtggga tcgatcgacg gcattgagcc ggggactgaa gagatctgga
    27961 ggtacgtggt gaactatccg gacgagccga tccccacggt gggccccgac cgctttccct
    28021 tggaggaggg agacgtggtg gttttctact tcgcagatag ggagaaaagc cccgagcagt
    28081 ctctcagagt ggagataacg acgggatggg agggatgaaa gcttgtccgc tcaccgacgc
    28141 ggcagaaggt ctgaggggtg ggggggtcct ggatgaggat ctcccccctt attttgctca
    28201 tcatcctgac gatcgtcccc gccctgggag acctcagcca gggcccctcc gagggagagg
    28261 cgaaagatga gccctccgat ctgatctggg agggcgaggt cctcctagcc ggcggggaga
    28321 tccccatatc aggcggaagc ggcaatccct cgatcgccga aaggtcggcc cttggagccc
    28381 tcatcagagc ctccgaagct ggcgggtttg agcttctggt ggacttgtcc ctctgggcga
    28441 tccgcggccc cgtagtggtg tctatgggag gcgcagatgc gacggcgaac agaacctgga
    28501 ggtacgtggt caactatccc caggacgccg tcccggtcgt cggagcagat caattcctcc
    28561 tcaaagaggg ggataaaatc accttcttcc ttggcgatcg gcactctctg cccgacgaat
    28621 ccccaagggt gaacatctcc gtgaggatcc tcacggagag gccggaggcc ctcttcatcg
    28681 ccgcagagaa ccacgcgggg atcgaggaat cggcgaagga cgccgtcctg aacgtcaccc
    28741 tcgccttcgt ggagaccgcc ccctcgaacc tatcgggcta cgacctgata ttcctggaga
    28801 tggtgggaag cgacggcgca agaaagctat tgcccctgct ggaggcgccg aaggaggcgg
    28861 gagttcccgt catctcgatc cactcggagg ggtacgacgg cctcctcgga gatgtcgacc
    28921 tccaggacca tcccgaggtc gaggagtact ggaactatgg cggagtcgag aacatgaagg
    28981 gactctattc gtacctggca agagcctttt taggcctgga ggtgccggtg agggcgcccg
    29041 ttccgacccc cagggcctac atcagccatc cggactcgcc ggacctcttc ctccacacct
    29101 cctcctacct ccagtggtac cggagccatt ccggccaccg ttacgacgag ttggctccga
    29161 cgataggggt gatgaacctg gcgagcgacc ccgtgacctc gtcgatgaga accgccatcg
    29221 tcagggctct ggaggcgagg ggggccaacg tcatcgacat cggatttgag aacaccacca
    29281 ccatccagga cttcttcatc ctgaacggat cgacgatcgt ggatgccgtt ctcctcacca
    29341 agcccttcag gctgaactac ggcgaccccg aggaggggat cgaggctctc aaggagctga
    29401 acgtccccgt cctcaacgcc atgaagctct ggtaccagac cccggaggag tggaggaacg
    29461 aaaccggcct attcccagcg gagctatact tcaagatcgc catgcccgag atggacgcgg
    29521 tcatcgagcc gatgatgata gcgggaagga ccgagaaggg aaccttcgat cccgtccaga
    29581 gccagatcga ttggctggca gatcgggcca tcgcctgggc agagctcggc cgaaggccga
    29641 actctgagaa gaaggcggcg atcatctact acaaccacgg cggaggaaag gacaacctcg
    29701 gcgcgaccta catcaacgtc ccccggagcc tccaggtgat cctcgagggg ctgaacgact
    29761 ccggctaccg ggtggagggg accgtccccg acgagcggga cctcgtcgac ctgatggccc
    29821 accagggaac gaacgtcggg acctgggcgc cggaagagct ggagaagatg gtcagagggg
    29881 ggaacgcgac cctcatcccg ggagacgatt acctggagtg gttctccgag atcgatcccg
    29941 caaagcagcg ggaggtgacg gagatctggg gcccgccccc cggggagatc atggtctacg
    30001 agaacgagtc cgggagctac ttcgtcatcc ccaagctctc cttcggaaac gtcatcctag
    30061 ccccccagcc gacgaggggg tggcttcaga acagcaccat cctttaccac aataaggaca
    30121 taccgcccca ccatcagtac atcgccttct acctctggct gaggaggggc ttcgacgccg
    30181 acttcatcgt ccacctcgga aaacacggca cccaggagtg gacccccggc aaggagagcg
    30241 ggatttcccg cgaccagtgc tggcctggga tcctgatcca ggaccttccc gtgatctacc
    30301 cctacatcgt cgacaacatc gccgaggggt cccaggcgaa gaggaggggg gacgcggtga
    30361 ttataaccca cctgacgccg ccgatagtcg cctccggcct ctacggcaac ttcaccaacc
    30421 tcgctgagac cgccttcaac taccaccagg tcgagaacgc ctcggtgaag gccctctaca
    30481 gggaggagat catcgcccgg tgcgaagagc tgcacctgga cgaggatctg gacaaaaacc
    30541 tgagcaagct agccggggac cccgaggcct tcgacggttt cgtcgacgag cttgagcact
    30601 acctctacga cctgaagaac gagttcatgc cctacggcct tcacaccttt gcaacgcctc
    30661 cggatggaag ggcccgtctg gagatggtac aatcgatgct cggcgacgac ttcaggagag
    30721 aggtcgccct catgatctcc taccaggact accccaaccc ctcaaggctc gacaaggagc
    30781 gagagctcga taacaccacc ctcggcctcc tggcggcggt catcgacgat aaaacgagcc
    30841 ccgatctcgc ccagggggag atcttctcca accgaacggc ggagaacctg accgcgctcc
    30901 tcgccctggg cctctcccgg tcggaggggc tcgacgcctc cgtcgaggag gtttcgaggt
    30961 tcataaacgc ctcggagtca gagtacaccc cgccttctcc ggccgacgat cccataaggg
    31021 atcccaacgt cctcccgacg gggaggaact tccattcgat aagctcgagg atggtcccga
    31081 cgccggccgc ctgggaggtg gggtcagccc tggccgagga tctgatcgag gtctatcgag
    31141 ccgagcacaa cgggacgtat ccgaggaagc tcgccatcgt cctctgggcc tgggccacca
    31201 ccgatcacgg cgtcgtcgag gccgagatcc tcaagctggt gggggcagag ccggtctggg
    31261 actcctacgg cggcgtctcc gacgtccggc tgaccccctc gtccgagctt ggaaggcccc
    31321 ggatcgacgt ggtggtcgtc ccctcgggac tccaccggga cctcttcccc gagaagctcc
    31381 agctcatcga tcgggcgatc cgccttgcag caaacgactc ggcgaccgac tatcccaact
    31441 acgtgaggga gaactccgag gagatcaggt cggagcttct ggcgacggga aactactccg
    31501 aggaggatgc cgaattcctc gccgccagca ggatcttcct tgaggccgcc gggacctacg
    31561 gcccgaacct cgatagcccc gtatccgcaa gcgacacatg ggagaacgat tcgaagctcg
    31621 gcgacctatt catcgacagg atgtcctaca tctacggcga cgggatctgg gggagcagaa
    31681 ccccctctgg caaggactac gcagcagtcc agaccgaggt ataccgccga aacctcgccg
    31741 aggtggaggc ggcggcccac cacaccaact cgaacctcta cggcttcatc gacaacgacg
    31801 acgtctacca gtacctgggc gggatctcca tggccgtcag aaccgtcacc tgcgagaccc
    31861 ccgacctcta cgtcaccgac gccagggcca agggcgatag gggcaaggtc gagcccctca
    31921 agagcttctt tacaagggag ctccggtccc gctactacaa ccccaaatgg atcgagggga
    31981 tgaaagggga ggggtactcg ggggcgaggg agatggacaa gttcgtcgag cacctctggg
    32041 gctgggacgt caccgtcccc gacctggtaa cggagacgat gtggaacgag gcctacgacg
    32101 tttacgtcaa ggacaagtac gatatgggcc tccgggagtt cttcgacgag aagaacccct
    32161 acgcatacca ggtcgtcacg gcgaggatgc tggaggcttc gaggaaagga tactggcacc
    32221 ccaccgagga gatgaaagaa aagctggccg aggagttcga ggagtcggag accgagtttg
    32281 gcgtcacctg ctgccaccac acctgcggaa acatcctcct ccgagagtac atggaggggg
    32341 tcctcaccgg aactgagcag acggaggcta ccggcgatag cgcgagcaag ggcggcgggt
    32401 ccagcaggca cccctatcct cgtgccgagg ctccaggggc cgcgaacgcc actcgcccgt
    32461 cgagcgtcgg gacagagatc ctcgaagagc cggtctcggc gggcgaggcg gccggatcgg
    32521 tgcaggatga cgtcagcggc ttagtcatgg aggagttgac cgaggagagc cctctcccat
    32581 cgcgatcggc ggccccgctg atggggatca tcctcgtcct cttcatcctc ctggcggtgg
    32641 gggcggggtt tctgaggagg tggtgagggc cccctcacct cccccctccc cggagaagca
    32701 aagctatctt tctctcagac aggctggctt gcgcaaatgt tatatcctgt aaacttgact
    32761 ttagtccaat ctagtgtagt aggtgaaagt cttggattac ccgtggcttg ccgcttctgg
    32821 tgttctgatg gggatcattg tattcgcgct gaaggtcagc ctggggtgcg gcctcgccag
    32881 cctcagccgg aaggagaccc tctccatcgg agcgtcttac ctggccctct ccctggtgat
    32941 gggcctcgcc gtcggcctcg tccctgaagc cgccatcacc tcgatcatga gcatgggggt
    33001 cgcgatgcac ctcggcatct ccctcctcct ggtggcggtg ggggtctcca ccgcccggga
    33061 gtggaaccgc cgcggccgcg acctctcccg aaagaccttt tggttcctct ccgtcccctg
    33121 cccggcctgc cttgccgcca ccttcctctc ctgcagcgcc ctcgctggcc tcctggacgt
    33181 ccccggctgg aaggtggggg cggtcgtcgg cctcatcttc ttcatctcca tcgcgattct
    33241 ctccgccacc ttcggaaggg tggggagggc cccctcggcc ctggggaacg ccatgatctt
    33301 cgtcggcctc ttctacctcc tatcgatact tctcatcccc gcctacctca aggccgcgtc
    33361 gacccccttc gtccccatcc ccatccccgc ctcggacctc atcacctcct acctattcat
    33421 cctcggactg gtcggcctcg gcttcgtcgg gaggaggatg ggggtcttcc ggtgaccgtc
    33481 tacgtagagg tcgtaaacct cctcttcgtc ttctcgaccg ccctcctcta ccccgtcatc
    33541 ctggcactcc tcctcctctt cggatggtcc ctcgccctcc tgggctccct catatccgag
    33601 tacacctccc ggaggagaga cctccaggag ctggagggga tctgcgctga ggcctcagag
    33661 atggcgctct cggggcggga agaggaggcc tcctacatcg cggccggcta ccgggggacc
    33721 ccccgggtca tggccgccct gggggaggcg gcccggtccc tccagggcga cgaccccgcc
    33781 gccccgaggc tggagaaggc cctccaggat gcggagctgg ggatggggaa ggccctggag
    33841 ccggtgaaga tcggcgttcg ggtggggccg atgctggggc ttatgggaac cctcatcccc
    33901 atgggtccag ccctgatcag cctctcccga ggggagatcc agcagatggc cgacagcctc
    33961 gtcatcgcct tctcgacgac agtgatcggc cttgccgtcg gcgggatctg ctacggggtc
    34021 tatacggtca aaaacaggtg gtatcgccag gacatgagcg acctcgagta cgcggcggag
    34081 ctcctgcatg gggagagaaa gagaaggtac cggagccgag agagggaggc agggggatga
    34141 gagaggcggg agggatggga ggcggcggca ggcgaggccg cccccgcctc ggagtcctgg
    34201 aggatgccga agatgacgat cccctgagcg gggtcgccaa cctcttcgac gccgccatgg
    34261 tcttcgccat cgccctcctc ctcgccctgg tcctctccta caacatcccg gagctcctca
    34321 accccgaggc gagcgtcacc atcgtcaaaa accccggcga ccccaacatg cagatcatca
    34381 taaaggagat ggaccagatc cagatcctga acatgaccga taagatcgcc ggggggcagg
    34441 ggacgaggat gggtacggcc taccgcctgg agaacgggat ggtcgtctac gtcccggaga
    34501 acgccacctc tggtgactag ggcagattga aactctcgta aacgcttctc cgaggcggag
    34561 tccctgggag gcgttgacgg cggccccccg tcagatcagc ccctcggccc ctctgacgat
    34621 cgccctcgcc gagacggctg tacccttgcc ggcgttgagg tttatggagg agaggatccc
    34681 caccaccttc ccgtcctgga agaggggggt cccgctctcc cccggccccg ggagatcggc
    34741 gtggccgagg acgatggaga tgaggtctcc gacgtcagat atgctgcagt ctctcgaacg
    34801 gcgggcgttc gcgaggcggg cggggccgag ctccggctcc gccaggaccg tcggctcgca
    34861 tcccgaggct ctgaagtaga cgaggtcgaa gggcttgcag tcggctacca ccttcgccct
    34921 ggccgttccg atggtgaggt ctttggttcc ggcgagcctc ataatgtggt agacggaaac
    34981 cccgatgcat ccgcttctgc ctttcagaaa agcgcccacg acccctccct gcctcccggc
    35041 agagaagagg tctccagccc ggatcatgat cagctttcta tatgatatgg ataaatagat
    35101 atcctgaccg atctgaccga tctgactaat ctctcggatc tgacaaatct gaccgaagga
    35161 gattcattcc gcgaaagctt gagaggatct catcatggct caccggatcg ctgaggatgg
    35221 aaaactggag gatgcgggga agcttcagct ttttattcac accctctttg cccgatccga
    35281 gccgacgatc ctcagaatcc tggaccggat gatggaatct gatctcttcc gggggagggg
    35341 caggccgatc ctcgtcttca tgcgggagag gctggacttc ctccctcggg gagaggtggt
    35401 gagcacccag agggcgaagg atttcctgga cgccctgtcc aactccggcg attcgaagat
    35461 catcgtcggc ccctgcgtat gccaggaggc cctggggagg agggagggga ccctcataaa
    35521 ggagctgacc gtcctctacg gcaccgagtt atacaagagg gcgaaagggg acgagggcgg
    35581 catcagagtg ataagcccgg ctgaggcgaa ggggttgctc gaccgtctcc gggaagaggg
    35641 gtgcatcccc accttctacg cctgcctcca gtccacgggc tggatggtgg tggtatgcaa
    35701 ctgcgatggg aaggtctgcc tgcccaagag ggcccaccag ctcttggggg gcgtcctcag
    35761 ccccgggccc gacatcataa gcctcggcgc cgacggttgc ataggatgca acagatgcgt
    35821 tgagaggtgc tcctttgggg ctaaccgcct cttcaacggc agggtcgtct tcgatcccga
    35881 gagatgctac ggctgcggcc tctgcgtttc gacctgcccg acgaaagcaa gaaggctggt
    35941 ggagaggcga ggccacgcca aactttacta ccccatcgcc ttcgtcgcca aatacggatc
    36001 aagagctgca ggcgatggtg aaaggccttg gaggtcttgg gccagcgaca tcggaggccc
    36061 gaagatccca tcaagacaaa attgatatca ggccgaagat ggagaccctg gtggaggagt
    36121 tttgtggtca gcgaaaagaa agccctatgg gtcctcttca ggcccaagga ccccgagaac
    36181 gtcgaagcga tgcgcgagaa gttcctcatc tcttatgagg ctttcagaga tatgccgggc
    36241 ctctatagca aggtctggtg gtcggacccg gagagggggg agtggggggc tctgtacatc
    36301 ttccggactg aggaggacct ccaggagtac ctccgctccg accggtggca gaagaagatc
    36361 cccga
//
LOCUS       DASM01000153            4114 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_41482, whole genome shotgun sequence.
ACCESSION   DASM01000153 DASM01000000
VERSION     DASM01000153.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_41482"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          732 a         1386 c         1233 g          763 t
ORIGIN      
        1 ggtccggagg aggccatcga cttgtggccg ctcccgacga tgacgtcggc gcccaggctc
       61 ttcgcggaga ccggcatcct ccccacggag taggcgccgt tcaccgccag agggaggccc
      121 gcgtcggtgc agatctccga gaccgccttg gggtccacca ggtttccgta gtttccgtcg
      181 ggataggtga ggaccgccat cccgaccctc tccccgcggc ccctcgcccg cgagatcgcc
      241 tcctggtagc ccccggggtc gacccggtac cccggcccct cgccggcggg gacgagctcc
      301 accttcagcc ccgccagctc cgcggcgagg agggtggtgt agtgggcgtt gccgtcggcg
      361 attaccgtct cccccccctg gcagagggcg tgcatcgccg cgaagatccc ctccctcgcc
      421 ccgtggctta tcctaacctc gtcgacctcg aggaactgag gaagggcctc gcggacgaac
      481 tcctttatcg ggggggtttg gatctcctcc agggacccgg tacaatagtc gcatacggag
      541 tagccgtcgg cccactccag gattgcctct ctcgcctcgg gggtgaggat cccccctcgc
      601 tggagggggt cgatgtttat cgccccagcc gtccccctct tcaggcattt gaaatgatcg
      661 agattgtcca gcattcctta tggagttggt agatcctgcg gtataaccct tcccctgagc
      721 cggatcttcg aaccgtcgac gagggcagat ccgaggcacc accaaagaaa gggtgatata
      781 tcttccccga ggacccccct tagatgtcaa gagtcatcgg ccaccggggc gcgaggtcca
      841 tcgcccccga gaacaccctc gcctccatcc gcgcggccgg agggtgtggc gccgatctcg
      901 tggaggtgga cgtccgcctc acaaaggacg gctttctggt ggtgatccac gacgccttcg
      961 tcgacaggac caccgacggc tcggggaagg tggaggagat ggccctggag gagatcaggg
     1021 ccctcgacgc cggccagggg gagagagttc cgaccctcgc ggaggctgtc cgcctcgccg
     1081 aggatctgga cctggggatc gtcgtcgaga tgaaggagga ggggctggag gatctcgtcg
     1141 tccgggagct ggaggggagg agggtgatcg tcacctcctt cttccacgga tcggtccggg
     1201 aggtgaagga gctcgggggg ctcaagaccg ggatcatcat atcttccctc cccatagacc
     1261 ccgtagacct cgccctatcg gcgaaggccg actcgatctt tccccggttg acaaacccca
     1321 acctcttcat cgcggcccgg agggcggggg tcgaggtata cccctggacg ataaacgacc
     1381 cggacgaggt ccgctggctg aaccggcttg gggccgccgg gatcgtcacc gacgatccct
     1441 gtggggtccg ggaggcggcc gacgacccgg tcacgaacgt cagagccggc gagtgccagt
     1501 attatccctg ccaccatttc caggggcagg actgcacctt ctgcttctgc cccctctacc
     1561 cctgcaaaga ccctgagctg ggaaggttca tcaggtcgag gcggggaaag aggctctgga
     1621 gctgcgtcga ctgcaccctg gtccacaggc cttccgtggc ccgatacctt tcagaccacc
     1681 ccgccgccac gaccgaggag ctgaagaggg tcgatagagc tggtggcgac tgaaatagat
     1741 atcatgggac cctgccgggg ggaaggggcc cccatcggcc gtacgtcgga gggtctgggt
     1801 ccggcgacgg aaagatttat gcacgaccgg tgtataaggg ccggattatg agaacgaatc
     1861 tagcattgct gatcgcagct cttgccctcc tgcaggtctg gtctgctatt gggcaggatc
     1921 tcaccctcag ttcgccggtg gaggcccctg cggccctcac cgacttcctg aacgaaacgc
     1981 caaatgccac cgtaaacgac accgccaacg gaaccgcgaa ccagtccata gtcgatacca
     2041 acctttcgac cacggctacc cagggcgctt tcctcgacct gcctgtcaac gagagccaga
     2101 tcgacaccaa catgacgacg acggccagcc agcaggcctt cctcgaccag cccgtgaacc
     2161 agagcacgat cgacatccag ccttcctgga cgagcagcat atacgccttc ctgatggatg
     2221 agccgatgga cgagcccgtc tctatctaca agaggggcca gctgatgagc gacgagccct
     2281 catcagaaac gccggatagt ccctacaaga ggggccagat gatgggggac ttcgattaaa
     2341 cttcctgggg caacccccct ttaaaatttg atatttagag gatgttatcg gaagatctcg
     2401 atggtcccgg gggatcgcga ggctgactaa tcgctcgcaa gtttcaatcc catatcgact
     2461 ccaggtgtag ccgctctctt cttaggaccc cgaccttgtc cttcgtcgac agggtcgcgg
     2521 gaaatttctt gtcgtccagc tccagggtga ggggtccatt gtatccagag tcccggaggg
     2581 cgaccaagac cgggtcgacg gatccggagc tcacaggaag gtgtcggact ccgtcgatgg
     2641 tcgcgctgac gtggacgtcc aggatccgat cccccagggt ctcgacgaag gccaaggccc
     2701 tggatggagg gggggtcgcg tgggagatgt ccagggtcat ccccaggcgg gggtgctcgt
     2761 ccaagaatcg gcccatctca tcgggctcgg agcagaggtt ccagggggct ggctccaggt
     2821 tctccagagc caggagaacc ccctcctcca ccgcccggtc gaggcagacc tccagatagc
     2881 ggcggagctt ctctcgctcc tcctccctgg cggggcgctt cgccgtcctc ttcccaggat
     2941 ggacgaccac caccctcgcc cccagaagcc ccgccagacg gatggcggat atgccctcct
     3001 ccatcgtcgc cagggagacg aggtcgttgt aggatgaggg gttgaggtcc atgacggggg
     3061 cgtggagggt cgttatcctc cttcccagca cctcccggat cagctccgcc ccctccggcc
     3121 gcctccctcc ctcccagtac cagggggtct cggcccagaa ctccatctca ccgacccccg
     3181 cctcggcgat cacctcggcc atcctccggg ggtcgaggtc ccagaggaac atggaggagg
     3241 cggataccct cgccaccctt caccgcctca gttcctctat cagccgctcg atcctcgcga
     3301 gccgctccat ctggacctgc atcctccgga ggaggaccgg gggggtctca gccggagcgg
     3361 tacactggtc ggtccagccg tccacttccc tcgtgacctt ctcgacgatc ccctccagct
     3421 ccccggggag cctcccctcc tcccggaggg cctcaacccc ctccatcagc gccgcccgga
     3481 tctccaccgt cttcagtagc accaggttca gctccaattc aatcctggcc tgatccacac
     3541 gtcttcctcc tcagccgtcc ttccgttgat cgccatcctg gagaggggga tcggatacaa
     3601 gcctaagcct tcaccccgaa ctgagcttca atatcgcctc tgccaggtct ccacctgcat
     3661 ccttcagggc tgccttcgcc tcctgggggg tggagcctgt ctgggccgcc accaggtcga
     3721 cgtcggagtc ggggatcgat atatcccggc ccctctcctg ggcggtgccg acgacctggt
     3781 aagacctgtt cccctgggcg tccatcaccg tcactgaggc gtcctgaaag aagatctccc
     3841 ggtccgccgt cctgaagatc acctcttcga ccccctcgat ctcctcgatg ctgatcccca
     3901 tctgcttcat catctgcttc atcttcttgg ggcttacccc cttgccaccg atccctggga
     3961 acatggtccc gccttggggg gcatccgtag taaatctatc tgtggccttc cgtcccccct
     4021 ctggggaggt ctcgggcgat ccaggggcct tctcccaggg acgagggggg atatcggagg
     4081 ctattgaaca aaaaaagatg gaaaaattgt ttta
//
LOCUS       DASM01000154            4647 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_175354, whole genome shotgun sequence.
ACCESSION   DASM01000154 DASM01000000
VERSION     DASM01000154.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_175354"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          875 a         1506 c         1380 g          886 t
ORIGIN      
        1 cgagcatcgc ctgcatctca ggagggcctg aggggaactc gaagtccttc acctcctcga
       61 tgccgaaggg cttcaggtcc gccagccacc acccctcctc catcgccacc atctcggcga
      121 cctgatgggt gctgggctgg tagccgatcc gcaaagtcgt aatctctgcc ttatccgaat
      181 cggtgcctag gcagccgagg gcgatgaggg cgacgaggcc caccgccagg gctgcgagaa
      241 taagatctct cctcaaaggt catcaccaga ggagggagga gggggtcaat atatagcatt
      301 ttccggtatg gccgatattt catcccaaaa gtactaatat ttagaaatta ttctacaatt
      361 agatgacata cgccgagaag ttgatgaggg cggccttcgc ctccgacgag aagctccggg
      421 aggctatgaa ggaggtcctg gcggagctgg ggctctccgc ccgggagttc agcgccgtct
      481 cctcgatacc ccagtccacc ctctacaaga tcatgtccgg ccaccgggag ccgaacatca
      541 ccaccctccg acagatagtg aagacgatcc gggatctcga aggggccgag accggcttca
      601 tagccgtcat cgccgccagg ccggtcctcg acgagatcaa ggagaagagg acgaggatcg
      661 gcgatcgcct cctcgtcctc cgggagtact cggcgacgaa catcgaggag gcgatcgtcg
      721 ccgccgtccg ggccgagagg gacggggccg ccgccctcgt ctgtgctcca atcgtcagcc
      781 cgacggtgga gaagctccta tcgatccccg tggcgacgat aatcccaagg gagagcctcg
      841 tccgggccat cgaggtggcg gctagaaaga tcgaatgaga ctggatctta ttcggacggc
      901 gggacgggtc gaggcccccg ctccatctcg taaggctttt atcgatgggg cgccgacaag
      961 ccgatataaa gggtattgtg gggtttattt atgagaacgg ttaccatatt ggcatttctg
     1021 gccctcgtcg ctctcagctt cgccgccata ggggcggagc cggtgagcgt cggaggagat
     1081 ttcggagcgg catggctgaa gaacctcccg taccagccca cggcggctac cgacgaaaag
     1141 gacggcctct ggagctgggg cggaacgccc cgttggatga ccatcgccaa cggaaccctg
     1201 gagaagctca accaatcgtc gaacgccgcg gattacctgt acccgggcac ctacggcagc
     1261 agcggtcatt ctgacgaccc atggatcgag gcccagctta cgggaaaggt tcagacgact
     1321 cttctgagcc agatgataag atataatcat acctccgacg gctgggtcgg cttcctgggc
     1381 cttccattgc ccccgtggta ctcctaatgg ctgttaagaa gatcagcata gaggctctct
     1441 tcagcagact gcaggaggtc cacccagacc tcaggctgac atcagcggag gacgtggtgg
     1501 tggcgaactg ggttaggctg aagtgcaggt acggctgcca ggcctacgga aggcacctgg
     1561 gatgcccccc ctactcgccg acgccggagg agacccaaag ggtgctggag gagtacagag
     1621 tcggggtcat cgccaggttt gaggcggtgg cggaccccgc cttcccgccc cagcggctcc
     1681 accaccacct ctggggttcg atcaacgccg tccacgagac gatattccag ctggagaggt
     1741 ccgccttcct cgcaggctac tacaaggcct tcgggttcaa cgcccttccc tgcaccttct
     1801 gcgagacctg catccccgag gagagggagg gagctatcga tctcaccgaa gtcaggaact
     1861 gcaggcacaa gaacaaggtc cgcccctcca tggaggcctc cgggatcgac gtcttcgcca
     1921 ccctggatcg ggcgggatac gaccttgcgg tcctcgactc cttctcgaag ggggtagccc
     1981 tcttcggcct cgtcctcctc gactgaaggc tgatcccaga gagagccctc ctgccatccc
     2041 ctcctatctc atctcatcct ctggaggagg cgatgaggag gtcggtgggg tcgaggagcc
     2101 cccggggcct tccgggacca aaccagtttc ctccccggta cctctccgag gattccggcc
     2161 gcgatgcata gagctccagg tggagcatgg ccgtcctccc ccggtccttc agtcctctta
     2221 tgtaggcggg ggctcgatcg tcgatcttca tggggttcag caccgatccc acccgcccca
     2281 ggatctggcc tggtcctatg cggtcgccga ccctcacctc tgcactggcg agctccgcca
     2341 gcttgaagaa acgctcctcc tcgtcctcga cgaggaggta gagggtctcg ttccagtagg
     2401 ggacggcccg ggggttggtg aagggcccca cctccaggac ggtcgcctcg ccgatggagg
     2461 cgacctttga cccgacggga gcgtagaggt cgacgccgca gtggcgccgg tctccccgat
     2521 cctcccagaa ggagcccggt gaccccgggg ccgggaccag cctctcgact ccggggaggg
     2581 gccaggcccc gccctccgcc gtcgagatcc gcctcataga tggtccccga gttccgcgat
     2641 cgagcccagg atcaggtcgg gagagatccc cgagggagcc accgcctcct cccggtactt
     2701 tccagtcctc accaaaatcc ccttcatccc gacgttcttt gccccgccga cgtcggagtt
     2761 tatgtcgtcg ccgatcatcg cctcctcttc gggggaggcg cccaggtccc ggagggcgag
     2821 ggcgaagaaa tcgggggagg gcttgcccac caactccgcc tttctccccg aggcgtactc
     2881 cagggcggcg acgaaggggc ccgcagcgag ggtgaggccg ccagccccca tccagtacct
     2941 gtccttctcg agagcgatga tccgggaccc ctccatcacc atccggaagg cccggttcag
     3001 ccggtcgtag gtgaaatcct cctcggcgtc ccccaccacc acgcaatcca ccccctcctc
     3061 ggctacgccg accccggcct catcgaaatc ctcccggagg cccggccccg caaggaggaa
     3121 gcaccgctca ttccgagacc ggagccgccc ggcggcggcg acggcggggg tgaagatctc
     3181 ctcggcgaag atctcaaacc cgagggctcc gaggaagccc gccagccccc ttctggactt
     3241 cgaggtggtg ttggagacga acctgcggcg ataccccctc tcctccagct gggcgagggc
     3301 ctccttagcc ccggggatgg gggtcttgtc cacgtagagg accccctcca ggtcgatcag
     3361 aaaggcctca gcatcgatcg ttcccatccc tcctggaatc tgaggtgatc cttcatccct
     3421 cttcaaggga ccggtcgaga actaagaagc tatcccatcg aaggggggga ggcagactcc
     3481 cgggggcgac gagaagagat ggggaaaggg agatgctgct ggccccacca tcgtcgccgc
     3541 cgcggaagag agagcctctc cacccatgaa tcctcctcgg tgggaggcga tgaggggagc
     3601 cggtgataag ggaagagttc aggcacctca accggggaga ggggttttta ccctcatcga
     3661 gggcgatgga gatgccaggt cggaaggtcg caagggggcg tcaggtcatg gacatcagct
     3721 cgaggcggac ccggtcctcc tcttcggagg gggactccgc ttccctcctc tcccccggct
     3781 catcgtcctc ggggatgtag gagaggtaga gcttccttct ctttccctgg gtgagcttga
     3841 cgtgggctgg gtccatatcc acagcccgct tcatcaccgt tatcaccgtc gtcttgttca
     3901 ggccgagcat ctccaccagc tcgctggtgg tgtacctccc gggccgctcc ctcaaaaggt
     3961 ctatcaggtt ctcggtgtgg ccgtcgaggg cgtttccttc agagaccttg ggagccctgc
     4021 cgacggagga cttcgcatgt ctagtcttca tctggctttt gagatctgcc acctcctttg
     4081 agaggtggtc cacctggtcg atgagcctcc ccaggagatc gacgtcctgc tgctttgcgg
     4141 tcagcatgtt ggagatgttc tccaccgcca gaatgaggcc catcatcgcc cggttctctt
     4201 ccagctcccg ctcactgtct gacactttgt ttttgtaaac ttccaaaagt tcttcaaaca
     4261 ttacttcaat tccccttata tctccccgta catcttgttc tcaatctagt ccccctctcc
     4321 cgaggcgtcg aaaatacttt aagttaacgt ggtcacaatc caagtataaa tagctttctt
     4381 taatattggg gatgagatcc ttccagcctg ttcatcggac cacaatattg tcaatgtacg
     4441 ggatgattgc cgatctccgg aagaggatgg aggccgatgg gatggtcccc cggggcttct
     4501 atcccaccgt cctcgagagc ctctccctca ccagccccgg aagcttcccg atgtcgtcga
     4561 tcacctcgtc cgccgcctcc atcagctcct taggcgggcg gctctcctgc tggaccgtca
     4621 ggacccccag gtctgccgcc ttcaagg
//
LOCUS       DASM01000155            4828 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_164768, whole genome shotgun sequence.
ACCESSION   DASM01000155 DASM01000000
VERSION     DASM01000155.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_164768"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          820 a         1593 c         1399 g         1016 t
ORIGIN      
        1 cgacggccgc gggcctcctc ccggtggaga tagccttcgc atcggcggcg gcggggatgg
       61 tcctggccgg ggtcctcacc atccatgagg cctacgagag catcgactgg cctatcatat
      121 tcctcctggg ggcgctggtc cctgtgggag cggccctgga gagcaccggc ggggcggaga
      181 cgatctccgc agccctcctc caggccctca gcccctttcc ggcggtggtg atcctgggcg
      241 ccctcctcac ctggactatg atcctcacaa acataatcaa caacgctgcg gcggcggtga
      301 tcatggcccc catcgccttc aacgccgccc tcgtcctgga cctctcgccg gaccccttcc
      361 tgatggcggt cgccgtcggc gcctcctgcg cctttcttac accgataggc caccagtcca
      421 acaccctggt gatggggccc gggggctacc agttcggcga ctactggagg ctcggcctcc
      481 ccctcagcct cgtcgccgcc ctggctgcct tacccctgat cctgtgggtc tggccgctct
      541 gatgggtggc gcagagcttt tgtgccaccc tgcaccggat cgccgaccta agccgcggat
      601 ccggctgaga tcatcatcct cggcgtgaac ccctggtcga ggagggcgta ccagaccatg
      661 gaggcgacgc ctcccttggt ggagtagacg acgacgggca cgtccttatc caggctgctg
      721 aaggcctccg tcagggcctg gtcgtcggca agcccctccg cccccatcac cttggagctg
      781 tcgatgttca tcgccccctc caggtggccg gcggagtact cctccgggct cctggcgtcg
      841 acgatctgca ggtcagagat ctcgccctcg acgatcagga ggtcgaggct agggctgggg
      901 ttgtaggcct cggccgtcct gacggcaggg gcagctacga agttgagccc agcagcctcc
      961 cgggaggccc gggtcccctc caggaggagg agctcctggt ggcccaggta cttcatcacc
     1021 cagaaggcga aggtgtcaaa cccgttgaag aaagagtcgc tgtaaattac cagggggtcc
     1081 tcctcggcga tcccagccgc cccgaagatg gccgcgatct cggcggggga cttgaggcta
     1141 ccgtcttcgg cgaagacctg gtccaggggt aggtggatcg ccccctcaat gtaggaggca
     1201 tctccgggga cgcctgcgta ggctacggcc agcctcctct ccccgttctg gtcggcgaag
     1261 agcgcctcgg gggatagcct ggaggccgcg ccatccaaac catcggcacc gccctcagct
     1321 ccatcggagt cgttccccgt tgtggtgctc gatcccgtag aagatgtcgt cgtccccatc
     1381 gatgagagct ctccagccca ggagatgccc atcgacgacg ctcccatcca tccggtggct
     1441 gggccgccgc cgcagctgga gcagcctgcg aaagctccgc ccgtcagggc ggctgccagc
     1501 agcagaaccg ctgcgatctt ggataacggt ttcatctggt ttcccatatt tgtatcctcc
     1561 ataaaatctc ctgatatcaa atccttctat tgagagatcc ctggagctgg aagcgacctc
     1621 tcggcggcgg gggaatatac ccgcgtctat ggtggcgatc atccacgatg atctatatct
     1681 cctcaggttc tgacccccgg ccacccctca accccgcgga tctggcgtat cggagtcgcc
     1741 atcgccccaa tcccctctct gtgcccctct gcccggggag caccacaagg atggaccccc
     1801 ccttggatct ccatatttat cacatattcg gtcgggaacc ttcatcgtct tcgtggagaa
     1861 ggtccgaggt ctcgggtccg gaggcgggga aaagaagata agtccgggga ttatacggcg
     1921 aatggtggca gccttcagaa ctttgtcgtc gccggtgatg cccctcgcct tcatcctggt
     1981 ggtcccagtc ctgggagctt tcgcggccca ggccgccgta gagcacccca tcggcgcggg
     2041 tcccgatgac tggtggatga cgtaccccga tcagcgttcg aatgctggat ctcccgtaga
     2101 ccacccctcc tgggctctgg agcctctgga ggaggggccg gtgatcgtcc tcatccacag
     2161 ctccaactgt cccgcctgcc tccagcagga gtcggcgatc cgaagggtct tggaggacct
     2221 tggagacgag gtggtctacc tcgacgttct gacggagacc gatcacctca aggcctggga
     2281 tgggcttgtc ctctactacc cgacgggcga tcctgggagc gatcccatct acatccccgg
     2341 gaccgtcttt ctgaccctgg ggcccgggac gggcggagag cccgtcgtcc tctggcacag
     2401 ctcggtcggg tctaccggag aggggcggat tcgatcccgc ctccaagatg ccatcgccct
     2461 gtaccaggag agctctcagg ggtgaggttc cctaatcccg cgagaggcct cccgtccgcc
     2521 tcctgcatcg ccgacggtat aatctccccc cggccgttcc ccgcaggtct tatacaatcc
     2581 aggtcgcctc ccctcatcaa aaggaataaa tggtaataat tcatagaccc ggctcataag
     2641 aacagggagg ttaggctatc tatgaaaata attttgatgg tgctgatcgt cgccatgctc
     2701 ctggtgggct tgggcgtcgc agccaaaact ccaattgatc tgagcactct gggcaagaag
     2761 gccgagatga aggccagcac gacccccgtg gtcatatcct acacccggga agcccccagg
     2821 accaccaccg acgccgtgga cgtcaggagc ctcggtagga gcttcggcaa cttcccgggg
     2881 aggcccagca tggaccccct ggagatcggc gctgccaacc ccataaagtt cacccccagc
     2941 ttctcgatct ccaccagcaa caggaccatg tccagggtga cggtctacac gccgcccttc
     3001 tccataggca acatcaccag gaccacccct cagcagatga tctttggggg ggcgtgaagg
     3061 ccccgagccg ggccttcgcg agatggactg ccggtcgctc cttggtaatg ggccttgaga
     3121 tggggggaga aggatgaagg tgacgcttct ggtggacggt catgccatcc ccatgaactc
     3181 cttcacccaa gaggtgatga tgaaggtggt atcgggggtt gccgagtccc ttcggggggt
     3241 ggacccccag tggaagagga tggtcgtcga ggtggaattg gaagatgaga cgggctgacc
     3301 tgcctcaacc ctttttggac tttttgaagg ctgaggagag gtctatgaag tacgtaccgt
     3361 ccttcagcgc catcaccagc tcgtcacgct caaagaggga ggagaggttc agctcgtagg
     3421 tccccggctc cgacatccgg atgctctcca gcatcttccc ctcgtcgatc cggtggttct
     3481 ttgattcccc ttccgggggc ggcggttctc tccctctcgt cgagtccaga tcgaagaacc
     3541 ccagagtccc cttcggtttt ggggtctcct tttgtttttc tctcccctca gcggatcgcc
     3601 tgctcctgcc cttcctcccc tttctggggg gttctctctt ggctggttcc gtcttggcag
     3661 gttccgtatt gggtggctgg tgcggggtcg ccttcaggat ctttcggagg gcgtcgtctc
     3721 cctccggcca gggctcgcag ggtccgccga tctccgaggc gttcagggcg ccccggacct
     3781 cctttatctt ctccttcgac ttgacgaata ggaacttctt ccagccgcag acgggacacc
     3841 cctccagaac gtccatgccg tcatcgaaga cgctcctaca tcgggtgcac atgtggggca
     3901 tgctggtcag tcaccgaccg agaccttcgt gctgatctgg tatttgtcct tctcgacggt
     3961 ccggagctgg tttgcggggc cgatgaccgt catcctcgtc ttgatccccg acttgccgag
     4021 gatcttgttc agaaacgtag attcattctt cacaggatag ctctcgatct ctatccccga
     4081 gaactcgtcg aggcgtatct cggtcatcgt catctcgatc agcttgacct cctcctcggg
     4141 ggtcaggccc agctccagca ccacgatgtt ccccctcttg accttgctca gaatgagccg
     4201 gatcttctcc atggaggaca tggactccag cctctcggcg gagatcagat ccatctgtac
     4261 ttcccgcatc atctcacccg aagtgcctag ctatggccgc atacagctca tcgatgttcg
     4321 acccctccag cgccgagatg gagaccaagg gatgctgtgg aaaggccgcc ttgatcctgg
     4381 aaggagaggc tttgggcagg tccatcttgt tcgcgacgat caggaggggg agctttctcg
     4441 cctccatatt tcccaccacc gtcacgttca cctgggtgta agggtcctcc gtagagtcca
     4501 tcaccagaag gacgccgtcc agatcttcca gccatttgat cgcctcgatg accccctccg
     4561 tcgcctcctt ggcccttctc ttggcctcct cctcctccag gccgtaagcc atgaactccc
     4621 tgaaatcgat cttggtggcc atccccggcg tgtcgacgat gtccagctcg acgctggatc
     4681 cgttggcgtt tatcaccacg ccgtcggtcc tcatggccct cctcgtctcg tggggtatgt
     4741 tggatatccc gcccacctcc atcccgctcc agtccttcac tatcctgttg gcgagggtcg
     4801 tctttccggc attgggcgga ccgtatat
//
LOCUS       DASM01000156           13660 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_22828, whole genome shotgun sequence.
ACCESSION   DASM01000156 DASM01000000
VERSION     DASM01000156.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13660)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_22828"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    11583..11663
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2710 a         4107 c         4091 g         2671 t
ORIGIN      
        1 cccatcatct ccagggcgaa ccagaggacc gccccgttca ccccggtgtt ggtgtagacg
       61 accaccggct ggtccttcct cagatctttg aagatcgcct ccagatcctc cgcacctttt
      121 atcttctcgc cttccaggat attttcggag ggtatgttga cggagcctgg tatcgatgcc
      181 gggtctgccg cctcggcggc cctcccatcc acgatctggg cttttccgct cttgacgtag
      241 tcgtgcgtgg cgaggagatc ggggttcacc tgggatacgt aatccacagc aggcctcgtc
      301 gccggagcct tgctggtggc aaggcccgcc gccttccagt cgtcgatgct gccctccagg
      361 atccggacat tctcgtggcc gagatacttc aagagccagt aggtgaaggt ggccgggaca
      421 ggtccgccgc cgcaggggag gcactcgccg tatatgacca ccgcatcgtc ccaagatatc
      481 cccgccccgc cgaggatctc gacaacctcg gagacgggct tagatctgcc gtcatcctcc
      541 aggaaggctt cgtagttgac gtttatcgcc ccatcgatgt actcgtcggc ctcgggactg
      601 atgtccagga ggacgtcaga ggcggtgacg ctttctatcg gggccagaaa ctccgatcgc
      661 ccatccgatc cgggatccac tcctttagag ccctcttcca tctggttgtt cgtcggcttt
      721 tctaacccag cggccggatc tgagctggct gacatctgag attcggccca cttggaccac
      781 gcctcgtagt tgttccagtc ggggcaggtg agacaccact cgccgttctc ggtcccggct
      841 gccgcgggac cgaccgaccc gctcaaggct gccatgcaga tgatcagggc gaaagaggct
      901 cctcttagag gcacaccttt aaatcctgtc aacggaagct gcttcataac aaaccatcct
      961 ttctccagaa gatatctcat ataaatccca aaatagatca tatcaattgc gtccagatca
     1021 ggggcgccag gccgaccagc gtcaggccgg tgacgagccg gatcgccacc cggtgctcgt
     1081 gcctgaacct gtccacctcc tggggagaca tgccgagggc tatgatcatc ccgataatca
     1141 gcaccggaag taccaccccc aggttgtaga ataggaggta gacggccccc gccgaggcgt
     1201 acccctggga ctggatgatg ccgatgatgg ctatgtagac cgctccgacg cagggagcct
     1261 ttatgaggga gaagagggcc cctatgagga agtaggagag gaggctgtac ctctccaggg
     1321 actttttgaa gtatcccaag gcccactcgg ccttgaagag ggagatccca ccccggtgaa
     1381 gcctccttgc gtcctcgatc tgggtggctc ccaggacgag gaggaggagg gcgagaatca
     1441 gccggaagct cgcctcaaag ctcggaatcg agaagagccg gaagagcccc aggccgaaga
     1501 ggtagtacat gacgaagatg ccgaggcaga agaacccgac catcaccgcc agatctcgcc
     1561 tccttccgga tcgcgagagg gtcatgctgg cgagaaatac caggaccgcc aggaggcagg
     1621 ggttgaaccc ggcgatcagc cccgccgcca ggaccgccgc cgccgacgag atcgagacgc
     1681 cctcaaggga gatgaggtcg tgatcctcct ttgcgggggc gggagctgcc tcgccgtcca
     1741 gagccgacct gatcatctcc tcgagcttcc gctcatctcc ttcgtagtcc cgatagccga
     1801 tgacgtggga gccgatgacg atcgagggca tatccttcgc cttcgtctcc ctgatgatcc
     1861 gccgcccctc ttcgctgtag aagatctcct cccggatggt gtagccggag taatcgccga
     1921 tgacctcatc gaggaccctg cccgcggcgg cgcacttgct acacccgggg gaggtgatca
     1981 ggatcgcctc ggagcttccg ctggcggcgg ccgctgatgc gatgatgagg tataaggcca
     2041 gggcgagaag ggcgcccttc ccccgccgga gagagcggac tggagacatc aatcctcgat
     2101 aataaaattt cggatggcgg gtgggaggga gatgccccgc catctcgctc tatcattacc
     2161 attgggatcc tggccgaggg gtttcaggcg atgtactcca gaactgcctt atcgtcttca
     2221 gacccttcca tggaggcaaa ccctatggag gcgtcgcctt ccgccaccag caccgcggtg
     2281 aaggggcagc cctcgctgca ctcttcggcc gccttcttca caagagacgt ggcgtcccag
     2341 gatgcccacc cctcctcttt gatctcgatg gtggccaggg gctcctcgtc atacaagggc
     2401 aggtgggccc aggtctccga atcgaggacc gccaaatcgt agaagtaaag ggtcacctct
     2461 cccggggtct cgacctcggt ggcgcggacc ctgagctccc cggattcgat ctggtctgcg
     2521 agcatcacca tctccttgaa tacgaggaag gttatcctca ccggatcgcc gtcctgggag
     2581 gtggcccata aagtatctcc gtcggcaaag gtctcgtcct cctcttctgc atccatatag
     2641 gtgtccaata ccagggccgg ggagatcgtc cccgccgccg ccacccctgc cagcatgacg
     2701 atgagggcgg ctgccagaag ccctccaaat ccaaagcttt ttttcttcat tttccccact
     2761 ccagacgaat ttatcctgtc ccccgcccat tgattcaggg agaggggcgg tacatttatc
     2821 gatgccaact taagcggcgg tcgggttttt tcccaacagt tatgaaacat aatcacgccg
     2881 ccaccatccc ataaatcatc cccatcaccg tcgagaggag gatgacgagg gagatgtaga
     2941 cggacgccct cttcgatccg ataatcctcc ataggacgat catattcggc aggctcatgg
     3001 aggggccggc gaggaggagg gagagggcgg ggccgggggc catcgccccc gaggagtagc
     3061 cgaacatcgt ccccacgatc ggaacctcga gaagggtcgg catgtagagg agggccccca
     3121 tcagcgaggc tataaagcag gccgcaaagt cgctggcccc gaagaaggtc cgtatcaggt
     3181 ccacgggcag atagtagcct atgaccccgg tgatgaaggt cccgaccagt agcagcggaa
     3241 agatcctctt ggcgagccac caggtctccc cgaagaagga ggccttctcc agatcggtga
     3301 agcgggccat cagaagccat gccgaagcca ggatcaggat cgagacgatc gccgtcttca
     3361 ccccgagggc gaggcctgag gtggcggcta ggaggacggc gacgagaagc ccgacgaaga
     3421 tcgggggtat gtaccccggc ctctccaggc ctgcctcggc tgatggtgaa ggagccggac
     3481 aggggccagc ctcttggtcc tcggatcctc tatcgaagat ggcggccatg atgaggccga
     3541 tgagaacaga gatagagacg gcggctagga tccgggcagc ccccagctcc agccccagga
     3601 cccgggccgt caggacgacg gccaggaggt tgatggcggg gcctgcgaag aggaaggccg
     3661 tggcgggacc gatcccggcc ccccggcggt ggatccccgc gaacatcgga agtatggtac
     3721 agctgcagac ggcgaggatc gtcccggagg tggaggcgac ggagtagcag agccacttgg
     3781 gggcgtcctt tccgaagtac ttcaggaccg cctccctctg gaggagagcg gctatagccc
     3841 ctgcgatgaa gaaggccggt atgaggcatg tcagaacgtg ggccgagagg tactccatca
     3901 gggcagaaaa ccccgcttcc agggcggaaa agagctccgg attcagagga tcactccctg
     3961 agatctttgg cgggtgaaag ctccgttaat ttatgccaac cccttaaatt tatatcttat
     4021 tttacacagt ccatcaggac cgactccgcc ttcttcagct cggatatctc ctccagggcg
     4081 agggagtcca cctctcgtaa gaggtcgaag agcctggggt ttttgaccct gtagagggtc
     4141 cgcttcccgt cgatcctcct ctccagtatc cccgcctcat tcaggatcga taggtgcttg
     4201 gagatggtgg actgggcccg gcggaaggcg gggacgatct cgcagacgca ccgctcctct
     4261 cctccgagga actctatgat ctcgagcctg atggggtcgg agagggcggc gaggatctta
     4321 acccgccagt tggtggcaga tcgtctcatc gtatcggggg tggagacgat ctagttctat
     4381 atatacatat cgccatgaga cgatttaaaa atccttgtcc cgggggggcc gtcgacgagg
     4441 gaaaggtcac ctcacccgct cgatcttcct agaccggcag gagggacaga agaggacgac
     4501 cccggtggcc tcaaaggcta tcctgcagcg gaggcatcga agatgcttct taaagaagga
     4561 ggcctcggcg gggtctatcg agcagccgca tatgttgccc atgacgtcat cacctcgccg
     4621 ggcggtcaga gtttcagcag tctcgcaaga tatccgaagg cgatcttccc cgcgacctcc
     4681 agcgatctca gggggttcca gccctggacc ccgccgtagg cggtggggtg gccctcctcg
     4741 taggttatcc ccgactcctc gtagacctcg tccgccacgc tcccgaggag ggagacgaag
     4801 aggatggcgg agatcccgag gacggcaagg cccccagccc ttccctggat agtcatatcc
     4861 caccaattat gcgccttcca ccagatatca gttcccctcg atagaggccg gatcttcgac
     4921 tgtcggaccc ggggatagat cgactcctgg atcaaagccc ggccgattcg aggataaaat
     4981 gatctaacgg ggccggtggg atgaagatca aaacctcgtc ctctcccttt ccgagaagaa
     5041 ctcggccgcc atcccggcga gccccagctc ctcctccatc ctccggatct cctcggcccg
     5101 ggcccgggtc atcggcctct tcggcgcagc ggtgcaggtt ccggcgtccg ccgtcgcccg
     5161 gtaggtcccg atcttccggg cgaggtcggt gatctcggac ttgtccatgg cgatgagggg
     5221 atggtagatg ggaaggtcga tccccgactg ctccgccatg atgttcgcgg gggtctggga
     5281 ggcgacctgg ccgagggagt agccggtgac cacgccgaag gcctcctcct ctgccatcac
     5341 catcccggct atcctgtaca tcagccgccg gcagaggagg caggtggccc ggggctcggc
     5401 tccggcgatc cgagcgaggc ctgccccgat ggggacccgg atgaagttga tcctcctgcc
     5461 ggaggtccac cggcggaggg cctcggccga cctctccgcc tgggggaggg cgtggacgta
     5521 aggccggggg tcgaagtaga gaagggatat cgggcagccc cgccgcatca tcatccaggc
     5581 ggcgacgggg gagtcgatcc ccccggagat gagggcgagc atccgcggtt gacttccgag
     5641 aggaaggccg ccgacgcccc ggacgacgtc ggtgaagacc aggaccttat cctcccgcgc
     5701 ctcgacgaag atctccacct ccggatcggt gaggtccacc gcggcgccgg tcctctcccg
     5761 gacggcgtcg cctacaaaga cggcgatctc ggtgctggat atcccgcctc cgccccgccg
     5821 ggcccggatg gcaaaggagc tcggcctccg ctctgctgcg aggtccgagg cgacggcggc
     5881 gatggctccc aaatccggct tgcaggtccg gacggggctc gtcgagacta cgccaaaaac
     5941 gttcgataag atctccggag ccctggggtt tgaggtctcg acgaagatcc tcccccgctc
     6001 cagggatgcc cgatgatcta tccccgcctc cttcaggtcg tggacgatgt tggaggcgag
     6061 ggtcctctcc cagctcttcc tggtccaggg gtccttgagg gcgatctcgc cgtacctgac
     6121 caggaccgag tcgaaagagg ccatgccggc ctttacctgg cggctatggc cttcgcggac
     6181 cgggcgatct tcctcctcct cgccccggag gaggtgtact tctggagggg cccggggttc
     6241 ttgtgagtcg actcggagtt gggcacctgc ctgatctgtc cttttcttcc cttgatcttc
     6301 gtccattcaa cgcttcctgt ctttcccatg ggccgtaact atcgggaccg atcttaaata
     6361 ggtttcgagg aacggagagg cgggagaggg aaaggtgggg gcaagagggg agtttaggtg
     6421 gtggatcggg agagggggag gagtgggtgg ggagccgacg ggagagggga ctcgtggagg
     6481 gggatggtaa agaacatgag atcgaaggga actcttataa cgggtcgggc cctcggaaag
     6541 agggtgaacc gcgagacggc catccccaag atcagggcga gcaggtcttc ggccctcgtc
     6601 ctcctgggga tgggaatata catcgcctac ctccgccgcc tcggcctagg ggaggtcgcc
     6661 gagagcctgg aggggctgag cctggcggcc ttatccgcag ccctcgccct cgccctcctg
     6721 atggtcggat tcaacgccct atcgtggagg aggatggtcg aggagctgga cctctccgcc
     6781 tccttcggcg acctcttcat gatatacctc tccagcatct tcgcgaacaa cctcatcccc
     6841 acggggagct tctccggcga gacggcccgg acctactttc ttgcgaggat cggcggagga
     6901 gggcggtacg acgctgccct cgcctccgtc gccgcgagcc ggatcatcac cgccgtcccc
     6961 ttcatcctcg ggatggtcct ggggatcggc tacatcaccc gcctccacgc cgtgccgggg
     7021 tgggccctca ccgcatgttt cgggatgatg gtcttctccg tatcggcggg cttcgccttc
     7081 gtcgggatct gctttgagga gcggtggatg cagaggacgg cggccgccgc cgtcggtccc
     7141 ctggagagga tattccgccg ggagctggac cggggggtct gctccggggc tgtgaccggg
     7201 ttcatgcgga gcatgatcct ccttcgggag aggaggggcg cgatcctgga ggctctcgcc
     7261 tgggccatcg ccggctggat ctctcttgtg atggtcgccc tcgcggcctt cgcctccctg
     7321 ggggtcgagg tctcgcccct cgccatctta gccgtctact ccgtggtcat cgtctttcag
     7381 accctgcccc tcggccttcc gggcggtatc ggcctcgtcg agatcctgat gaccgccctc
     7441 ttctccgcca tcggggtccc gatccacgat gccgccgccg tcaccatcct catccgcctg
     7501 gttcagctct ggttcctcgc cctcctcggc ggcctctccg ccttccacct catccggcgg
     7561 atcgagaggg gtggggagga gccggctccc ccggcgggat cgaggggcgc cgggatctga
     7621 aatagagtta aataccatcg aggcgtccag ccctcccggg cgggatagta gggtagcctg
     7681 gtccatcctc gagcgtttgg gacgcttgga ctgcggttca aatccgcgct gtcccatcaa
     7741 atgcttatct aaagcaactc gcgattctgg cggtcagatt ccaagtccct ttcctttcct
     7801 tcccttccct tcccttccct tacacccaaa actttttgcc gccaggggcg aagaccttcg
     7861 accggtggag gaactgacga gatcgaagcc ttcagccgtc ctggcgctgc tgatgctgtc
     7921 gctcgccgcg actgcgctgg ttcaagagga gggaggcgag aaaggcgctg aggacggagg
     7981 atatatgggg ggatggcccg aggaatttga gcatatatgc tcaactctat agaatttgaa
     8041 tagaaatact caaatcgttg ctatttgagc ctatatgctc aaactttcaa cttgccgaag
     8101 cttcaccgcc agatcgcacc ggatgattat gaaaaaccca ggagaaagat aggatcaagg
     8161 ggcagggtgc ccatcaactt caactcgatc tccacggcga aaatctatat aacgcttaac
     8221 tgaagagaga cgaataaaaa tctgagaatg gcaataatat ggatgaaaaa ttatatgcag
     8281 tattaaccgg cgatttgatc gggtcccggc agcttaaaag cagcgggatt tcttatattg
     8341 aacatcttaa taaatctctg gaatatgtgg gtagggaata cctgcttccg ccttttatct
     8401 acagaggcga cagctttcaa gggatcacca acgaacccga atctgctctc aaagatgcga
     8461 tcctcttgcg gctgcggttg atctcgggct ttaacgtaga ggagaaattt cctcgcatcg
     8521 atgccagagt ctcgatcggg gtgggcgaga tcgatcgtct cccagatgag ggatatcgcc
     8581 ttggcgatgt tggagagatg aacggcgagg ctttcgagta ttcaggtctg cagctggatc
     8641 gcataaaaaa tgagaggcga aatttggccg tcaaaacgcc ctgggaggag acgaaccagg
     8701 agctggagat acactgcaag atgatagatc ggctgataga taggtggacg aagaagaagt
     8761 gtgaggctgt aatgtatcgg cttgaaggcc gtaccctgaa ggaaattggc gagatgctga
     8821 acatcgatgg gtctgccgtt cattaccggc ttgcacagac cgactacgag atcgtcgagt
     8881 tgatcatcca gaggtactcg gatgtcatca gaaataaggc cgagatgtgc gggtcggacg
     8941 aagaaccggg gttcaggtag agtagcatga ctgcgatctc ggacctctca gggctttcgg
     9001 gcctctcagg cctttcaagc ctatcgggcc tgttcttcct gattcttttg gtcttctcgc
     9061 atatgctggc ggatttcgta ttgcagccga atcgatgggt cgacggcaaa tatgaggatg
     9121 gatggaggtc gaggtacctc tatctccatg tctgcgtcgt cgcccttctt gcagggtccg
     9181 tcgcctgggc gttcctcggc ctctttccgg ccgctctcgt cttcgctagc tttttcttat
     9241 cgcatctggc gatcgatatg gtgaagtcga gatgcgaaga tacggcaagg tcgtttgtcg
     9301 gcgaccaggc agcccatatg ataattataa tattagtttg gataacatta tttcatccgg
     9361 atttctcctc acttcagggg cgcctctcgg agccgacgtt gatctgcatc accgtggtgc
     9421 tgctgagcta cactttgatc ttccagccgg cggggatctt cgtatcgaag atgaccagag
     9481 gctggcgaga taagatccag gaagacgata aaaataaggg ccgcgagagc gggagcctgg
     9541 agagcgcggg cagatatatc ggctatcttg aacgtttttt aattttgact ttcataatat
     9601 ttcagcaata taccgccatc gggctcctga tcgctgctaa atcgatcttc aggtataata
     9661 cgaatcgaat aaccggcgag tacatcctat ttgggacgct cctcagcttc agcatagcca
     9721 tcttcgtcgg gttttttgcc aacctcttcc tctccctctt tctgcccgac gaggtctggt
     9781 atattttaaa tacctttata tataaccctg cggcgctgcc atgatcggcc ccggcgcccc
     9841 ctggcggcac aaccccgccg ccggcgcgat ctctcctttc ctgcaacgag ggcgcggggc
     9901 ctcttcaccg atctccgggc ggcggcgttg ggcggggcta tcggaagtcc gaggaactcg
     9961 cccaagcttt ttgtagattg agattgaact gaatcatcgg aggggtcccc tacgagatcg
    10021 aagcctttcg ctgtcctggc gctgatgatg ctgtcgatgg cagcaaccgc gctggcccag
    10081 gtggagggag gcgagaacgg cactaaccaa acgggtttta gaaatacaac gagcatcgaa
    10141 tcaataggac cagagatctc caatggggct ccttggtatg aaaattcttg gattatcgtc
    10201 gttggtttaa tcgcttcttt gataacgatt ggatcactca tattctggat ctacacaaat
    10261 ctgatttcga agggtaaaaa taaaactgag ctagaaatta aagtttcaga gtcaagttcc
    10321 gctgttagga tctccaacat cccccacccc cacaacccca acttcaccgg ccgcgtggac
    10381 ctgctggacc gcctctccga agccctcgcc tccggagagc gcgccgcctt cacccagacc
    10441 tcggccatca ccggcctcgg cggcgtcggc aagacccagc tcgccctgga gtactcctac
    10501 cgccacgccg acgactacca ggtcatctgg tgggtccggt ccgaggagcc cgccacccta
    10561 gccgccgact acgccggcct ggcattgagg ctcgacctcc ccgagaaggg cactcaagac
    10621 cagagggtca ccgtcgaggc cgtgaggcgc tggctggagc agaacggcgg ctggctcctg
    10681 gtcttcgaca acgcccagga ccctgaagag ctgaacgatt accttcctcg gaccggcgca
    10741 ggccacgtca tcatcacctc ccggaatccg aactggggcg gcaccgcaaa gcctctccca
    10801 gtagacgttt tcagccgaga ggaatctgtc gagttcctca agaatcgaac tggcggccag
    10861 gaggacggcg ccgacaccct ggcagaggcc ctcgggaacc ttcctcttgc cctggagcag
    10921 gcgggggcgt acattcagga aactggaatt tccctctcgg cttacatcaa gctcttccag
    10981 gagcgccaga aagagcttct tcgccgtgga aaacccgacg cttacccaga tactgtggcc
    11041 accacctggg acctctcatt ccagaaggcg agcgaggagg tgcccgccag cgccgatctc
    11101 ttgaagctct gcgctttcct ggctccagac gacatcccaa agtcgctcct gaccgacggc
    11161 gttgagcacc tgcccgagtc cctggcctcg gccgtgaaag acgagatgaa gctgaacgac
    11221 gccgtcgccg ccctgaagcg gtactcgctg atgaccgtcg ccgacgactc cctctcggtc
    11281 cacaggctcg tccaagccgt ggcgcgggat cgattgtccg acgtggatcg gaaacgatgg
    11341 gcagaggccg cggtacagct cgtgaataag gtcttcccct atgaaagcta tgatttccgt
    11401 acctgggcaa agtgctcgat tcttttgccc cacgccctcg ccgctgcggg acattctgag
    11461 gagcttgagg tggcccatga ggcgaccagt cgccttttga atcaagcagg tttgtactta
    11521 agaggacgtg ccgagttctc agaggccaag tctgtacacg agagggctct tcaaatcgac
    11581 gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnnn nnnnnnnnnn nnntgtcaac aacctcggcg gggtcctaga agacctgggc
    11701 gacctggccg gcgccaagaa gagttacgag cgggctctta agatattcca gcattttctt
    11761 ggcgaagatc accccaagac gaaaaccgtg cggaagaacc tggaatccct ccgccgataa
    11821 tctcctacac caccacgact tcgtggcaga ccccgcacgc ctcgacgatc cggtcgtact
    11881 tcatctccgg gaaggggtcg atcaagcaga gatccgtcgc ctccgcccgc gagaacagcc
    11941 tctcggcatc gccgaagtag acctcgatcc tgcagccgcc cccccaggcc tcagccaaca
    12001 ggaccgattc cgcgccgatc tctccttctg gggtcttgtg ccgctcgatc ttctcgatcc
    12061 ccagaagtga ccggttcgcc tccaggttga ggatcgcatc ctccaccgcc gggagccagg
    12121 cgtcgttcag gacgacccgc tccgccccgg cgagggcggc gacgaggccc agggtcccag
    12181 ggccgcagag gacgtcggcg acacgggtga gcttgccccg gaagtacagc tcctccagga
    12241 tcttcatctt gggggcgctc tgtcttggaa actcgacgtg tatctttgac tgatgcttgt
    12301 agatgaccag atcgcctatc aatgaggaga cgacgtcgca gcggaggtcc gagcccgcca
    12361 ggagctccca gcgggtgggg ggagcccgga ggtctgcgac gccggggacg ccgccgcccc
    12421 cgatgacccc cttgatctcc gggacctggg cgacgatctc ggcggcggcc tctctcgtca
    12481 ggccctcgcc gacgatgatg agctcatccg gccccagcct cggcgggtag gccagggggt
    12541 agcccacctc caccaggggc cagccgatgg accggagggg gtcggttggg cttcggaggt
    12601 tgtggtcgac gaggacctcc atcgcctcca ccatcaccag gtccaggggc gccctgccgc
    12661 agccggaaca ccgcttccgg cgcccttcca tcgcgggttg ttcggaaaag ctcgcgccct
    12721 tatcgagggc gggctctttc gggcaggctg ggcaggccat gtaaagcgca tccaggcggg
    12781 ccaaaacatc ctcggcaggc ctgatcgtct cgcccttccg gggcttgcag accatgaaat
    12841 ctcttagaaa ggggtcccta tttcagcctt tccaggccca ttttgggagc gtttcgggga
    12901 cgtttcgtag aaagatttgt aaacaaaaaa gacgctcccc ctggggagga ggtttttaag
    12961 atggcacaga gagggatcag ggaatacgac gctaagaagc tcctggcgga gctccttccc
    13021 gattatgcgg acgatttcga ttacgatggt cagatcgccc ttgtcacccc agaaaccgac
    13081 ctggaggtgg tggcgatcaa aaacccctgg gtcaagaaga aaaggctggt ggtcaagccc
    13141 gaccagctct tcggaaaaag gggcgctcac ggcctcatcc ttctcgacgc ctcctgggac
    13201 gaggcgaaga ggtacatcga ggagaggatg aacagagaga ttctggtggg gacggtcgtc
    13261 gggaccctcg acacattcct catcgagccc ttcgtacccc atgaacctga ggaggagctc
    13321 tacgtcgcca taaagtccga gagggataaa gatgtgatct acttctccct ccggggcggg
    13381 gtcgagatcg aggcgaactg ggactccgtc accgagatcc ccgtcgacgt cctggcgagg
    13441 cccgaggagc tcgacctcgg atcaaagctt ccggcagatc tcggtgaaag acgaacgccg
    13501 gtgtcagact tcatcaaggg gctctaccgg ctctatcgag acctcggctt tgcgtatctt
    13561 gagatcaacc ccttcgtctt cgccgaaggc aagatcgtcc ccctggacct ggtcgcccgc
    13621 ctcgacgacg ccgaggcctt ctggcagagg aagacctggc
//
LOCUS       DASM01000157            3222 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_419149, whole genome shotgun sequence.
ACCESSION   DASM01000157 DASM01000000
VERSION     DASM01000157.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_419149"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    381..573
                     /estimated_length=193
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          503 a         1014 c          919 g          585 t
ORIGIN      
        1 ctccaaggcc ttcgagattc tgacggtcgc cgaggcgagg cccgaggaga tggtggcttc
       61 ggtgaagatg ctcgccaaga cctgcgccgc catctgcgag gggcagtggc tggacatgga
      121 gtttgagagg cgagacgagg tgaccgaggc cgagtatctg gagatgatcg agaagaagac
      181 cggggtcctc tacggcgctg ccgcctggat gggggcccgc ctctccgggg cggctgagaa
      241 ggaggccgat ggtctggaga ggttcggccg tctggtgggg atggccttcc agatccaaga
      301 cgacgtcctg gatctgacgg cccctgccaa ggtcctagga aagccccggg gcggggacct
      361 cgccgagggg aagaagaccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgataag cagaagttat taatccatat
      601 aacgaccaaa aggattcgta gccgggcgct gtcggggtcg ggtcaggcca gcggctggag
      661 cgccgtcgcg gcgatattag ggaggaggcg ttcaaattgc acgagcacaa gcacgggtcg
      721 gatacgcacg ggtcgcatga gcacgagctg aatgcgcacg agatcgagca ccttctggat
      781 cactggatcg agcacaacaa gagccacagc gagagcttcc gggcgnnnnn nnncggcgga
      841 gctgatggac gcgtgcaccg cgaagctcga ggaggcggcg ggagagctgt gagcgccaga
      901 gggaggagga gagggcctct ggcgatcgat gctcggggta ttcccagcag atgaggccag
      961 atgctccaaa acccccctct gaagggccga gacgaccggc agcttcacca ccaccgccag
     1021 cctctcgcct cctatcctgg tgatcacgat cgactccttc ccgtcggcga tcatcatgca
     1081 atcgaggctg tagtttacgc cgtccaccct aatatccttg tatatctccg cagctccccc
     1141 cccatatctc ccggagatct aaccccagat cctcgaactt atcctttctt tctatcgact
     1201 atcatcacga gcttgcaccc ctctttcagc tcggatgaga gatcggatct gtgctccctc
     1261 aagaaatccg ggcttctgca gaatattaag agctctccat ccgccctttt catgaggtct
     1321 cggaccctgt ttttgatccc ccactcgttc cgaacgcacc agacgggggt ggctcctgga
     1381 gagtccaggg tcaggttctt cagctcatcc gccgatcggt ctgctgcctc caggagcccg
     1441 acccgacggc cttcggcgtc atcctcctcc tgggggcggg agggctcatc ctaggcggct
     1501 tcttatcgaa cttcgccaag acggagtttc aggtcgtcca gttcataccg atagtcatcc
     1561 tcccccaggt cgtcctttca ggggtctggt ggcccctcga atcgatcccc gacttcataa
     1621 ggccgatctc ctacatcctc cccctcacct actccagcga cgccctccgg gcggtgatgc
     1681 tgaagggggc gggggtatca gagatcctgg tgccggacct gctgttcctg gtcgggttct
     1741 tcgtcatcgc cttcgcggcg gcgacgctga tgctgaagcg ggaggtgggt tagtcaaaac
     1801 tgtctcgact cggatggggt ccagagccga gatcggagag atcaagttcg gatccccccc
     1861 tccagcctct cccttgccgc cttcttcacg ctctgatccc catcccccac cagagctctc
     1921 acgacctctt cgggggtgga cggattttgg gccacagcct ccctcacctt ccaggcccag
     1981 tccccggaga gggccaccag ggtcgcggga tcggcgatcg gattcgacgc caccgccgcc
     2041 cggatctcga catcccggtc cgcggcgagg ctcgcgagtg tggaggccga cgtctttttg
     2101 ttcatcgcta cggcggcccg gacgtcctgg atccggtcgg cggcgaggta ggccaggagc
     2161 tcgacggtcg cctgcgggct cctcgccgcc gccgtcctga cggaccagga ggggtcgatc
     2221 ctcctatcga agaggacgtc ctcggggacc gtcgggtttg aggcgacggc ggcccggacc
     2281 ccctcgtctc cgtggtcggc gagggacctg atcacctctt cagggctctt cgggttcttg
     2341 gccgcagcct ccatgacgat gtagcggttc ttcgagaggg aatagtcaaa gtccatcgtc
     2401 tcgtcctcgg cgaggtccga gaggatctcg ggggatgtcc tgggattgag ggcgacggcg
     2461 gcccggacga agccgtctcc gtcctcggcg aggagatcga ggatctcgcc gtctgcctga
     2521 gggcttgcgg ccaccgcctc ccggacccac tggaccctat ctctcgccag ctcccccagg
     2581 atctccttgg gcgcggcggg gttgttggcc gccgcccggc ggacgtgcca gtcccagtcc
     2641 ctcgcgagcc tctccagctc ctccccatct atatctgggt ctgatgcctc cctctccttc
     2701 ttccagtccc tcatggccag atcctccctc cttcgtaacc ggtcggctct tcatcctcct
     2761 tttataggta tccaccttct cctccccccc gcgagccgct ccatttccca agtcttcgat
     2821 ccgaagggag ggcgtcggct catccccgga ggcaagagaa ggcgtagacc tcctccagga
     2881 cctcggccgg gtccttccgg tcgatccggc cgaacttcgc ctccaggatc ggcatctcga
     2941 tggcagggcg gatcatccgc acgagggcgc tctcgccgcc gacgacggcg tagacgggga
     3001 tcgacctccg cccctccagg aggctctcca gcctctccga gacgagggct gcatgctggc
     3061 ggatatcctc ctccctcagc cgctcgaacc tcctctggga ccagccgccc ttggtgtgct
     3121 tctccttgac ggtgctcttc accaccgcct cctcgatgaa gccctccctc cccagggcca
     3181 gccccaggag ggtctctccg gcgtgggccg ccaccaccag ga
//
LOCUS       DASM01000158           16349 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_122523, whole genome shotgun sequence.
ACCESSION   DASM01000158 DASM01000000
VERSION     DASM01000158.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 16349)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16349)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16349
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_122523"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    14475..14497
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         3734 a         4533 c         4734 g         3323 t
ORIGIN      
        1 attggaattc caaaatttgc aaaatttgca tttggatcaa agagcattgt acttattggt
       61 ccccagataa taatcatcca tataaatgta atgagggatg taaaaagtgc atattttaaa
      121 attctagata aaatagtctc taaatttcct tctgtgtatt ttaatctctt tgtaagatac
      181 aatcctactg gaaacccgaa taatatgcag aaaaatggaa ctcccattcc tattccaacg
      241 aagaatgtgt ccagtattat gtaagcagca aaagcagctt ttttattgat tttaaaaatt
      301 ttattcattt actttcctca tgtaaagtat actttacata atgttatata aactttacta
      361 tctgtaaaga agataagtca tcctcagggc tgatcggatc ggcgaccgag ggagaaggtt
      421 tccaccaaaa gagaaatggg gtggtggcaa agatcgcgag atctggatga ttaagttgtg
      481 ttagttaagt ttaaataact aaatgtaaca caagatttac atcgtgatgc cgatggacgc
      541 aaagacctcc aggatctgca gaatggtcgt catcgtggcg acggtggccg tagccggctg
      601 gtctgccgtc atcgccgagg tccccctggt attgatctcg gcggcgacgg gcgggtttct
      661 cttctacctc tgcagatcga ggatggaaga ggtggtcgtc gacgagaggg acgagagggt
      721 gagcgagcgg gcggctaagg tcgccgtgga ggcgtttgcg gcggcgagcg ccctcgtcgg
      781 cgtattattc atcgccgcca gggatgagca tcccggcctg atgaacgtcg ggtttgccct
      841 ggccttctcg gcctccaccc tgatgatcct ctactcggct ctgtacggat actacaaccg
      901 acgatacgga tacgagccct gcgatgagga ataagatcaa ggtcttcaga gcgatcttcg
      961 acctgaccca ggaagagctt gcaaagaagc tgggggtgac gaggcagacg ataatcgcca
     1021 tagagaagga caagtacaac ccctccctcg acctggcctt caagatggcg aggatcttcg
     1081 gcgtcaagat cgaggatatc ttcgtatacg atgaggagct gctggaccct gaagagagag
     1141 ggggctccgg ccggggaggc gctccccctc cgagctgatc ttaagaactg accaaaaagt
     1201 ttaacggcga agggaaccct accgagctcg atccccttga aggagcaggc catgtccgaa
     1261 gttcgaggcg ccagatcctc cagaaggccc atcttcacca gaatcaggtc ggttttgacc
     1321 tccaccaggt tcagcgagga gcacctggtg atggcggcgg ccctgatggc gaccctcctc
     1381 atcctcctct acgccttcgg cgccttggag acgaaaggag aggtctggag ggcgaccttc
     1441 ggggggccgg gcttcgacgt cggatcctcg gtcctggaga aggccggagg cctcgtcgtc
     1501 gccggcagga ccaactcctt cggcgccggc gggtacgacg gctggctcat tgcgacggac
     1561 tcgaagggga gggagctctg gaaccggacc atcggaggcc ccgggggcga tgaggcgacg
     1621 gcgatcgtgg cggcccgggg cgggggctac gccatcgccg gggggacggg atccttcggg
     1681 gcaggaggct ccgacggctg gctggtcagg accgaccacc agggccggga ggtctggaac
     1741 agaaccttcg gggggaccgg agacgactgg gcttattccc tccaggagac gggggatgga
     1801 tacgtcatcc tcggggaaac cgattccttc ggcgccgggg gaagagacct ctggctcctc
     1861 aaggccgacc accagggccg ggaggtctgg aacaggacct tcggagggac cggagacgat
     1921 ggcggccgct cgataaagag gacagccgac ggcttcatca tcgcaggctt caccgagtct
     1981 cgcggcgccg gaggcaagga cctctggctc ctcaaggccg atccgaacgg gtcggaggtt
     2041 tggaacgtca ccttcggggg atcaaaggac gacatcggag aggcggtgat cgagagagtg
     2101 ggcggcgggt acgtcgtcgc cggaggggcg agcgttcccc cggagggggg gttgggggac
     2161 gcctggctga tcataaccga cgaccagggt agccccctcc gggggaagat cttcagcgtc
     2221 ggggactccg gaagagacct ggcgacgacg gtggcggcga cggcggatgg gggctacata
     2281 gtcggcggct ggagctccgc cggaggactc tactcctggc tcctgaagac cgacggggcg
     2341 ggggagaagg agtgggactt caccatcccg gattcgggga gggaggaggg gtcctcgctc
     2401 ctcgtcgcag aggacggcgg gtacgtcacc tgcggctgga cgatctcctc aggaaacccg
     2461 gatatgatcc tggtgaaggt caaaaggtag cggaggggcc ccttcatcct gagaggctct
     2521 atctatcggt gggaccactt cgccctcccc ctcctccgag cttcgtcatc cagagatcga
     2581 agcctccccc tgagccgcgg ttcgtgaacc cgccgacgac gtagccaccc tcctccgcca
     2641 ccaggacgga gatcccgctc tggatcccat cccctcccag gaggaccgac cactcggccc
     2701 tcccctccgg atccgtcttg aggaggaggg cgtcatcggg gctcgccctc gttcctgccc
     2761 gggaggtcga tcccaccacc gcgtatccgc catccgaggc cacctcgatg gagcttccga
     2821 agtccgccgc aggggttccc gccgtcctgt tccagacctc caccccctgg aagtcagcct
     2881 tcaccagcca gatctcaaaa cctccggagc cgaaggagtt ggaatagccc gctccgacgt
     2941 agccctcctc cgtctcccgg agggaggaaa ggccgtcctc ccccttcccg ccaaaggtcc
     3001 gggcccactc ccaacgaccc gatccgtcgg ccttcaccag ccagccgtcc tcctcgccga
     3061 cgccgaagga ggtggtcctg ccagcgatgg cgtaaccccc gtcccgggtc gccaccaccg
     3121 ccaacccctc atcgtccccg gggcctccga tggtccggtt ccactcctcc accccccggg
     3181 ggtcggtctt gatcaaccag aggtcaacct tcccgatgcc gtaagagtcg gtccatccga
     3241 cgacgacgta gccgccggag ggggcctcct ccaccgacca ggccgcctcc tccccggggc
     3301 cgccgaaggt cctctcccac tccaggttcc cctccgcatc ggtcttgagg agccagacgt
     3361 ccctaccccc ggcgccgaag gaggtggtat acccggcaac gacgtacccg ccgtcccggg
     3421 cggcgatcac cgactccccc acatcgacgc cacctccgcc gcgggtaaga tcccagagct
     3481 tgctaccgtt ccgatccgac ttcaccagcc agaggtcgcc gtttccagag ccgtaagact
     3541 cggtcatccc cgtcatcacg tagccgccgt cttgggccct cgagatcggg ccccccacct
     3601 cgtgccccgg ccctccgtag gtccggctcc actcctcctc cggcgcccca ttcgcggggg
     3661 gtgcgaggag cagaagcagg aatcccaata tcagccccat cctcaaggct catcactccg
     3721 atagaggaga gatacctcca ggttctttat cgtatccact ccgaagaggc gccggcgaaa
     3781 ggaggatcag agccccggac cattcggctg gtccctccgc cttcggaagg atgacccgaa
     3841 agacattata gccatggcat agcataacat ttagaggttc gccgtcttcg cggctggagc
     3901 tgatccatga tccgagttct gatggtagac gacgaccccg ccatcctgga gatcgcgaag
     3961 atattcctgg gcaggtccgg agatatccag gtcgacggcg tggcgtcggc ggtggaggcg
     4021 gtcgcgaggc ttgaggacgg ccgctacgac gtcatcgtct ccgattacgt gatgccagag
     4081 atggacggcc tatccttcct caaatgggtc cgaagccggg acccggaggt ccccttcatc
     4141 ctcttcaccg ggaaggggcg ggaggaggtg gtgatcgagg ccctgaacaa cggagccgac
     4201 tactacctcc agaaggacgc aaacccgaag gtcctctacg cggagctgac ccaccagatc
     4261 cggcaggcag cggagcggat gaggaccaaa aaggcggtcg tcgagagcga ggagaggtat
     4321 cgggccctct tcgagaatat gaacgagggc ttcgtcctct acgagatcct ccgcgaccca
     4381 gaggggaggg ccaccgacta cctcgtcagg gacgcaaacc cccagatcga gctcttcacc
     4441 gggtggaggg cgcaagaggc catcggaagg agagcctcgg agatcttccc ctcagaggtc
     4501 ccccccttcc ttgagatata ctcgagggtg gacgagacgg ggtgccccgc caagttcgaa
     4561 ggctgcttcc cggagacggg gaggcacttc agcatatccg ccttctcgat agggaggggg
     4621 aggttcgcga cgatattctc cgagatcacc gagcagaaga ggatgaacga ggagctctgc
     4681 cgggcccatc atcagcttca ggagatcata gacttcctcc ccgacgccac cttcgtcatt
     4741 gacgagaaga ggaccgtcat cgcctggaac cgggcgatcg aggagatgac cggcgtctcc
     4801 aagggggaga tcgtgggaag gggaccctac tcttacggag agcccttcta cgggtttgag
     4861 accccggtct tgatcgattc gatcttctcc gaggactccg ggatagtgaa ggagtacgat
     4921 catctcagcc ggaggggaga cgccgtcttc gccgaggtct acgtccccca cctctacggg
     4981 ggcagggggg cctacatctg ggcgatcgcc tcgcccctct tcgacgacga cgggaggatc
     5041 gtcggggcga tcgagtcgat ccgggacgtc accggtagga agcgagctga gctggccctc
     5101 cgggaggcga cggactacct ggagagcctc ctccactacg ccaacgcccc catcgccgta
     5161 tgggggccca acatgaagtt catccgcttc aacagggcct tcgagcggct gacgggctac
     5221 gccgccgagg aggtgatagg caaggaggcg acgatcctcc tccccgacga ggagagggaa
     5281 gaggccctgg cgaagatggc ctacgccctg gagggggagg ggtgggagtc ggtggagatc
     5341 cccatccgtt gccgggacgg gaccgtcagg atagtcctct ggaactcggc gaacatccgg
     5401 tcggagaagg acgggaccgt cgtcgccacc atcatccagg ggcaagagat caccgagagg
     5461 aagctcgcgg aggagaggct gatccaggcc cacaagcagc tgctggagat catcgacttt
     5521 ctccccgacc cgaccttcgt catcgatggg gagaggaggg tgatagcctg gaaccgggcg
     5581 atcgaggaga tgagcggcgt ccccaaggac gagatcgtcg ggaaagggga ttacgcctac
     5641 ggcgaggtct tctacgggac aaaaagacca atcctcatcg acatgatctt ctcgaaggac
     5701 gaggatctgg agtcgctata ccattacgtc aagagagagg gggataccct cttcgccgag
     5761 gcctacgtga ccccccctcg gaccgggagg gggtcttacg tctgggggac cgcctccccc
     5821 ctcttcgaca gggacggaaa tatcgcgggg gcgatcgagt cgatccggga cgtcaccgag
     5881 atcaaggaga cggagatggc cctccaggag aacgaggagg ggctgaagct cgtcatcgag
     5941 ggggcgaacc tggggatctg ggacaggaat gtggagacgg gggaggtggt caggaaccgg
     6001 cggctggtgg agatcctcgg ctgctcagag ggcgacctgg tggggaacgt ccgggagagg
     6061 gaggagatga tccaccccga cgacttcgag ggcgtgatgg cggccctgga cgaccacttc
     6121 gccgggagga ccccccacta cgagatcgac taccggctga ggcgaaagga cggccggtgg
     6181 atatgggtcc acgaccgggg ccaggtgatg gctagagacg gagcgggccg ccccctgagg
     6241 atgacgggga tcacccagga tatcaccgag atccggaggt accacgaggc cctccatgag
     6301 gcgaaccgga agctgaagct cctcagcagc atcacccgcc acgacatcca gaaccagatc
     6361 accgccctct ccgggtacgt ccacctcctg agggagggga tcccccggga cccggccgcc
     6421 gagaggtaca tcgaccggat cggggaggtg gcggagacga tcaaaaggca gctcgccttc
     6481 gcccgggact accaggagat gggggtcaag gcgccggagt ggcagaggat cagcgaggcg
     6541 gtccggcggg cggcggccgc ctcccccgcc gagatatccg gtgggcaggt ccggctcgag
     6601 gtggagacgg gcacggtcga ggtcttcgcc gatccgatgc tggagaaggt cttctgcaac
     6661 ctcctggaga actccctccg ccacggggga ggagtcagcg gggtccgggt caccttcttc
     6721 gagagagaga ggggcgtgga ggcggaggat ggtggaggag gaggcgagag aggagggggc
     6781 gtggaggccg agggggtcat cgccgtcgag gacgacggcc tggggatccc cgccgggatg
     6841 aaggcccgga tcttcgacca ggccttcggg aggcacaccg gctacggcct cttcctcagc
     6901 cgggagatcc tggggatcac cgggatgacc atcgccgaga acggcgagga ggggcggggg
     6961 gcgaggttcg agatatctgt accgagggag aattataggc gggggtagtc tttccggaaa
     7021 gagtcgaact ggttttagcc attaagaaat cctgatagac acctttttaa atagtattag
     7081 ttcatttatg tgaagatgat atcatgatga catcacaaga tgcgacacca gcatatcggg
     7141 gttatcgact tcaggcacta tatacgtttt ttcgaatatt agattctgaa gatggtgcaa
     7201 aagctgtatt tcaaccagaa ataatggaag atcttgccgt cttagatgtt agcggaaatt
     7261 tgctagaagt catccaggtt aagtccaata gcaaacttac cttatcatcc tttaacccaa
     7321 aaagtgaact atcctttttc tatcgagcaa acgcctattt gaagactgat agtccacccg
     7381 aaattcatat agccagcttt ggagaaattg gaccatcatt actccaagcc attgaaaacg
     7441 atggcattga acgagaacgg gaagcagaaa agctgagtag ctatggacac gtcaaaaaag
     7501 acgatgcaaa atatatatta gataatataa atatatttca gatagatgtt agtaaattaa
     7561 ccgaatctgt atacgaacat cttaggaatg cccttaccgg tattgatccc gataacgcat
     7621 ttgacttgtt gagttattgg ttgtatatct gtgcagagaa taaatacaaa atcacaaaga
     7681 acgacatcat taaaaaagtc gaagatatag gaaaatttct agcgaaacga gccgcatatc
     7741 actcagagtg gtttacatct attataccga tagaagatta cgatatcgaa aaagaagaat
     7801 tgcgtatact ttctgatgaa ttttataagg gtgtttcagc tagatacgac cacatattag
     7861 ctgacttaga tgtttttagg cttcagaaaa tgctggatat cgattccaaa tttaaggatt
     7921 gccgggttgt cgttattcat ggagcttccg gccagggcaa gagtactttg gcctaccgtt
     7981 atcttcatga ttctttccct aatatgtggc gctttaaagt tgaattaatc gagaacagaa
     8041 agcacgcact cagcatcgca agggcactca taggacatgc tgacgctata gatataccaa
     8101 ttgccatcta tctcgatatt tcagcaaaag ataataattg gcccgaattg atcagacaat
     8161 tatccgttca tcggaatatt cgtattcttg ttacaattcg tgaagaagat tataatcgtg
     8221 caagtattcc cgattatgaa attcaattct ctgatattga tcttactttt gacaggtcag
     8281 aggcgcaggc gatataccat catcttgcca taaaaaagac ccctacagat ttcttgagtt
     8341 ttgaagaagc atgggataaa ttcggcggcg aaggaccgct aatggagttc gtccatttgg
     8401 taacccaagg tgagtcactt cgaaagagac tgaaacaaca agtaaatcgc cttaaggacg
     8461 aagcaagatc tgctagaggg gatatgagtt ttaaggaaat agagcttctt agattagtat
     8521 ctatagcttc agctttcgat tctcaactaa aggttaagcc gcttgtggac tatctaaaat
     8581 taccagtacc tcagagaacc tttgaattat tcgaaaaaga gtacttactc aggcttaacg
     8641 aggatggcag cttggtgggg ggtctgcatc caatcaggtc agaaattctc gtcgatttaa
     8701 taactgatcg cgtgctttct ccttggtcaa aatccgctag catttgtttg ccgatgatac
     8761 aagaaactga ttttgaaagt ttccttcttc acgcgttctc acgccgacgt gtagaaatcg
     8821 aacccctttt agacgcactt aattcttatc agccagataa atgggcggca ataggaggaa
     8881 tcaccagagc atttctctgg cttggaatac aggaatatgt tgaagtaaat agagaactaa
     8941 ttcaagaagc attcgaaaaa tcgggaaccg gttttatatt tttgttagat tctgacatat
     9001 ctgatgttgt gccagatatc atgacatcga gcttgaatac ttttatagga ttaggcataa
     9061 ttaataaagc taatcgtccg caaatagagt ccttcagaac ccgccaaacc gataagatgc
     9121 aggtttttgc gagggctcag acatggttgt taaaaagaac tctgtgtcct gaaattccgc
     9181 aactcgatgc agattggtct gggatggcag agaccgtttt ttggttaggt caccttaaca
     9241 tatcgtggcc tctttttgac tggttgccag atacagaact tgatagagca attgacgaat
     9301 taccacttga aacactttca gatgttatgc tggggataca ttatggatat ggagataaat
     9361 ttgctaaatg gttagaatcg aatcgttcta ggattataaa ccgttttcga gaggagacac
     9421 taacggtcaa actccaagat tatgagaata aggtatgtgc acattttata attgattatg
     9481 aaaaattaaa caatcctaca tctggtgatc tccagaattc gagtaactac cttcacgacg
     9541 aagcaatacg acgagttaag ttattgagaa aactattacc tgatcgagaa acttatgctt
     9601 gtcagggtta cggccaacaa ttgtggaatg aaacgcttcc gattgatgat acgcaaaaaa
     9661 ccggaattcc aaaatctaac cttcctccag cctggttgac atcgatcaac tcgacattca
     9721 gagggttggc agaaatgccc tatagaccca aaaattggga agaatatgca aaatcgatct
     9781 ttgaattgag aaaatgtgtt ttaagttcta tgaaagaact tgaaagcgtt ttacagacat
     9841 atttccgtaa acaaaaacca gatcaatggt ttagtaaact gattaattca aaagaatgga
     9901 ttcataacca acaaatgtta ttaaagtctc cgcttattcc ttcttgtgct attgatgaat
     9961 ggggatttat ggatgagttt ttatcagatc aaatgacgaa aaatgttctg aataccaaag
    10021 acaatttaga cgtaagagga ctcgcccttc aaaaacacaa accatttttg aattctttta
    10081 ggaaatacga tcgaagttta tcaaatttct ttaatcaatc aagccatgta atggtactga
    10141 attcaatacg tggaaaatgc gtgcggggtg catctgaaaa ggccagatct gaaaagaagg
    10201 caatttcggg aggggttaag cctgaatata tccgatcatc aacaattaat ttatcagatg
    10261 ctgtcaaagt gttacccaca tttcaaagaa tattcagata tgcattcagc caattcttcg
    10321 agatttatga tctctacgaa gttgagaaac aagaagagga cgtatttaac cgagtgttta
    10381 gcttgtggta tctctttagc atcgatccaa atctgacaca tcgaaaagca gaaagagagt
    10441 ttccgaagaa cgtaactagt agaacaaacg ctattttgaa aaatattcaa aaatcatttt
    10501 cagatgtttc atcagccgat ctcagtatat ctatccaatc atccagaata tattgggacg
    10561 atgtccgagc gctttggatt tcaatcgacg gaaaaagcgc aatagatgta tacaactctg
    10621 ccgaaaacgc gtttcccttg atttttaatt cagttaggag acttaacgat tttgatttga
    10681 ctcgtcttgt ttttgaattt acctggccct caatagtagt agttccaaag ttacaaggta
    10741 aatgtctgac agataaagca tggcgcatca attctaatat ccttctattg ggaggagaag
    10801 taggtaagct gagttggtgg aattttctaa ttctacatca aattcctaaa gatgccttag
    10861 ccgaactaaa ttttgatatt tgggatgata cacatctaga agttgtgact aagctgcttg
    10921 gaaatgcatt tgatatgtca tttagacttg cacatatccg agatcttgga aaactatccg
    10981 aattggacga acagggaatt aatcaatttg gcacgtatgc ccaaaatatt atgaataaga
    11041 ttagcataga atgtacaact ctatcggatt cgagcttgaa aatgatgaat gttttcaaag
    11101 aattgcccga atcagaatac gaaaagcatc aagatgctat taaatccatg caagcattga
    11161 ttgaaatgat aaagtatatt cccggaacca acggtgaagc aatcgttgat tttaagacga
    11221 tagaacgatg ggcaaatatg ttagaaaaag cacgagaata tgctgccgta gcctacttgt
    11281 tctgggcatc tgatgtaatt tctaacatgt agttcctttt aaacaatcgg ttatttgtaa
    11341 aatagttggt atttttacac ctcgcactcc gccaggatct cctccgcgag cctcctcatc
    11401 ctctccgttc ccgcaaattc ccccaccagc cggagggcga actccatcgc cgtccccgcg
    11461 gccttgctgg tgacgagctt tcctgagacg acgacccggt cgtcgacgta ctgctcgtcg
    11521 gacaggtcct ttctgccggc ggggtggatc gtcgcacgcc tcccctccac caccccggcc
    11581 ttgatcagaa ccgagggcgc gccgcagatg gcggcaacgt actttcccag accatccatc
    11641 cggcggacca ggtcgaggac ccgctcgtcc tcgcccaggt gaacgaagcc cggatagcct
    11701 ccggggagga cgacggcgtc gaaatcctcg gggtcgatat cctcgagggc ggcgtcgggg
    11761 acgattctga tcccgtggga cccccggacg gccccgacct cgtcctcgtc ggccgctcct
    11821 cccctcctgg cagatccatc tccatttctc ttgccgaggc ttgcggtcac cacctccacc
    11881 tcggcccgcc tcaggatgtc gacgatcgtc acaaactcga tctcttcaaa gccctctgcc
    11941 atgggaacga cgaccttcaa gacctagagc ctccagcttc tcctgacttt acgctaccaa
    12001 aggagctttc agccttcgat ctcttataac tttccaagag atcggagtct tgcgggattc
    12061 cctcatcatt ccactctcct caggtcgaag gcccaccggc tgacgccggg ggcgacgttc
    12121 ccgcacctgc ggtgatgctc aactgttaaa ccccggccct cgaactcccg gatgagcccc
    12181 tcgacctgat ggcgtttctt gaaggtgtaa aagtggatcg cgccgcccgc cttgacgagg
    12241 gggcttatgg cctcgaggat atcgtctctg ccgtaggggg tggggacgac ggcccggtcg
    12301 aaccgcctct ccggaggcca agccttcccc cctccccccg accccatcgt ctctccctcc
    12361 ccgggagctc tccctgtcgg ggcccggcag aaggcgaagg cgtcagcgtt cactatctcg
    12421 atcccatcgc cgaccccgtt cagctttgcg ttctccgcta gccaccggca cgcctcgggg
    12481 ctgatctcca gggccaggac cctcgccccc gccgctgcta tggggacggc gaaggggccg
    12541 accccggcga aggggacgat cgcccgctct ccggggagga ccattgatgc gaccctcatc
    12601 ctctccccgg cgaggcgggg gttgaagaag acccgggcga cgtcgaggcg gtaagaatag
    12661 ccgtactccc ggtggcaggt gagggtgccg ccgccggcga ggacctcgaa ctcggcgacc
    12721 ctccgctccc cctccacctt tgagatcttg ttgaggacgg tcctgacgtt cctgtgagag
    12781 gagatgatcg cctcgccgat ccgcctcccc tcgccctgcc gggccggaga gatcgagacc
    12841 accgccacat cccccaccag atagaagctc tcgacgtccg ggcggacccc gccggaggca
    12901 gagcctctcg gaccgccctt ccgcctcata ccccgcctcc gctggcctga ggggggagtt
    12961 cataggcgga agccatctgg cttttactca taaaggccgc ctggtatcgg tagcagataa
    13021 agccgtcgac ctgagtcctg gcgggcagga gtccggagac ctcggcagca ttccggaaag
    13081 ggatcggggg tcaggttaag tagttttaac gccaaagagg ggagaaagct ggcgagatcc
    13141 gggggggtct ggagacggcc ggcctcccgg ccgccgacgt ggtggaggca gatccagttg
    13201 aacgagaagg gcgattttaa aggggtgctt aggggggagt cgatcgccta cttctcgatg
    13261 gagatcggcc tggacgagag gatccccacc tacgccgggg ggcttgggac ccttgccggg
    13321 gacacggtca ggtcgagcgc cgacctgggg attccactcg tcgccgtcac cctggcgagc
    13381 cggaagggga gcttccggca ggagctggac gagagcggaa gacagatcga gcacccggcg
    13441 gagtgggacc cctcggagct ctgcgacctc ctgccggaga gggccatggt ccgggttcgg
    13501 ggacggcccg tcgccgtcgc cgcctgggag cagatcctga agagccccac cggagggtcc
    13561 gtccccgtcc tcttcctcga caccaacctg gtggagaact ccccggagga cagggggatc
    13621 accgacgtcc tctacggcgg ggacgaacgg taccggctga agcaggagat cgtcctgggg
    13681 gtagggggga tgaggatcct ggaggccctg gggttctcta tccagaagta ccacatgaac
    13741 gaggggcnnc gtcttcacca cccacacccc ggtccaggcg ggccacgacc aattctccta
    13801 cgagatcgtc cgggaggtcc tcgggggagg ggcgatagag atgctgagga ggttcggcgg
    13861 ccgggatagg ctgaacatga ccctcttcgc cctaaacctc tcaatgtacg tcaacggcgt
    13921 cgcaaaaaag caccgtgaga cctcgcgatc gatgtttccg ggctacgtga tccactcagt
    13981 gacgaacggg gtccactcct actcctggac ctgcccctcc ttccgggagc tctacgaccg
    14041 ataccttccc ggctgggcga acgagccgga gatgctggtg agggtcggga tcatccccga
    14101 cgagaagatc gtggaggccc acgccgctgc caagagggag cttatcgatt acgtgaacag
    14161 gtcgacgggg gcggcgatgg accccgaggt cctgaccctg gggtttgcga ggcggtcgac
    14221 ggcgtacaag aggccgaccc tcctcttctc cgacctggac cggctccgga ggatcagctc
    14281 ccggggcgag atccaggtgg tgatggcggg gaaggcccac ccgcgagacc tccagggaaa
    14341 gaggctgatc gaggagatct tccgggtcgc ccgggccctg aagggcgaaa tcgaggtggc
    14401 ctacctggcg aactacgaca tgattctcgc atcgaagatg atctcgggtg tcgacgtctg
    14461 gctgaacacg ccgcnnnnnn nnnnnnnnnn nnnnnnnctc gacggctggt ggatcgaggg
    14521 gtggatcgag ggggtgaccg gctggtctat cggccccgga cccgaggagg acctaccccc
    14581 tgtggagggg aggctaaagg atacagagga cctttacagc aagctcgagt acatcatagc
    14641 tccgaccttc taccaccgga aggacgactg ggtgaggatg atgaacaact ccatcggaat
    14701 gctcgcctac tacttcaaca gccaccggat gatgagaagg tacgtcaccg aggcgtacct
    14761 ctgaggatcg ggagcgttca tcatagccgg gaggctttgg tggagatcgc cgggcctggt
    14821 cccaggagcc ggaggggacc tctatgaggg agggcagatc ggtgaagggc gggggaggag
    14881 gccgcctggt atcgccaacc ggggtggctg ggcagcccct cagcggtagg ggtcgacctc
    14941 tagggggctt tcagcgatct cctccagcct ccccttcagg tccgagaggt ctcctgagag
    15001 ccgatcctcc aggagggcga ggctccgcct ccggtcctcg agctccgcct ccagggatga
    15061 gatcctctgg cggaccaggt cccgcttcgc cttcagccgt gccgtatcgc cccccctctt
    15121 cgagaggctc tcttcgagct cctcgatctc ggccgtgagg gcggagtggc gggccttgag
    15181 gagatggagg ggacgatcct ccaggaggtg gtcgatatgc tccatcaccc tctccctcat
    15241 cttatcccgc aggcccagga ggtccacctt cgacttcagc tcagagagcg cccccgacgc
    15301 atccccctcc agggcccgag ccggcgcgac gatcagaagc tcgaagacct cccggtgctg
    15361 gagggctatc cggtcgctat catcctgctt caccgcccga gagagggcct tggcgagggg
    15421 ggcgaccagg tctctcatgc tcatctcgat ctgatccctc tccctcatct tctcctccag
    15481 ggacctcttc tcctcccggc ccctcctccc ctcgtcgctc cgggaccagg cctcgatctc
    15541 ctcctggtgg cccgcctcct catccctcag gcgggagagg agggcctccc catcggagat
    15601 ctcggccctg agcgcctcga tctggcccct cccctcccgg accctggcgg cggccgaggc
    15661 cgcctctcta tgggccccaa ggctctcctc ccgctcctgg aggaggtcgc ctaactccga
    15721 taggtggcgg ctgatcgccc ccaggtcgga tttgatcgcc tccacctctc cggggaagag
    15781 ggcggcggtg tacctctgag ccctaccgaa cttctggacg gtgttctgga ggagcttgag
    15841 gatcgtcgag tggtagtcct ccgccgaagc ctgatccgcc ctccggggtg gggcgagctt
    15901 ctcgaccagg accctcatct gccggaccac cgtctccctg gtggcggtcc ccgccttgag
    15961 gaggcggggc ggggcctcct ccgccggctc cgccgactcc agggctgaaa ggtcccgctc
    16021 caggtccctc gccaccccct cgatggaggc gtagagggac ctgaccccgc cttcaaaccc
    16081 cgagtcggcg gatcggtcgg cgatccaccc atcgacgcgg gagaggtcta gcttcggcgg
    16141 ctcctccgcc ggggtcggcg ggggcgactc cttcgaaaag atctttttta gccacttcaa
    16201 ggccgatccc acctctcttc cagagaggga tgagacctta taaactatcc gacctatagg
    16261 ctcccaaaag tcttaagcag gggcgggcca atcccagcga ttatgaagtt cgatccagcc
    16321 cgcattcggg aggctgccgg cgaggactt
//
LOCUS       DASM01000159            3742 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_212127, whole genome shotgun sequence.
ACCESSION   DASM01000159 DASM01000000
VERSION     DASM01000159.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_212127"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          627 a         1179 c         1131 g          805 t
ORIGIN      
        1 tccgccacct ccatcatggt ggtgtacccc gaccggctga cgacgaacct cgcccggttc
       61 agaagctcca ccttctcctc ggtggaggcg taggccatca ccgtcgtccc atcgtcctcc
      121 ctcctggtga agctctcctg ggggcttccc aggaggatca cctttcttcc ggggaggtct
      181 tttacctggg ggaggacgat ctcctcgagc ttcgtcctct ggggctccgg ccctgagatg
      241 atcaacagat agtccaggtc ctcttctagg tccatcttcc tcgggctgca gaggatcccc
      301 gagtagaagg ccctcccctt cgtcgccgcg aggaaggagc gggagagctt gccgctgagg
      361 gtggcgtccc ccgcctggtt gtcggggact atgacgccgt cgtacttgct gtgaaagaac
      421 ccgttgacga agagggtcag gatctccacc ggccagaggt aggcggggac gctgaacctc
      481 agctggtggg ttatgaagaa ggaggggatc tccctggagt agaccccgag ccggttgtcg
      541 ctgatgatca tgtcgaagtc gcctttcgag aggatccggc agaggttcct cctctcatcg
      601 acgagggcct tcatcatcat ggggatgtaa gcggtgaact tggggaggaa gaaccgagtg
      661 gagctgtagg gggcgggata gtcctcgaag gtgagaaaat tgcactgggg aaactccctc
      721 ctcaggagct ccagggagtt cccgctggag gcgacggtca cctgatgccc ccggtccagg
      781 agctctctta cgatggggat gtcccgggag gcgtgaccta gcccccagtt caaagggctg
      841 acgaggacgt gggccatgat atgatcgaga tgggccgggg atttttaaat ccttttctgc
      901 catctgctcc ggagagatct ccgcgatcct cctccgagga ggaccatacc ttatggcaaa
      961 aactatattc atgtgacgaa aacaattatt ttctccgtca agtaaaaatc ccggatggta
     1021 gaagataact atttctaagc ctaacgcctc caggccggcg tggtccaaga cttagagctt
     1081 cctgcgataa atgcgagacg atcgacagct ctcgtcgtcc tggggctctt cggatacttc
     1141 gcgtacctcc gccacctggg gctcggcgag gtctcggcaa gccttgcgca ggtgaacctg
     1201 gcggtcttcg gcctcgcgtt ccttctatcc ctgttggaca tcgtcttcaa cgccctctcc
     1261 tggaagaccc tggtcaggga gctggactat gggatctctc ttctggacgt cttcctcatc
     1321 tacatgtcca gcatattcct caacaacctg atcccctccg ggagcgtatc gggggagacc
     1381 gcccgcctct acttcctctc caagatcgct ggcaacgcga ggctcgactc cagcaccgcg
     1441 agcgtcgccg ccacccggat catcaccgcg atccccttcg tcctggggat gtccgccggc
     1501 cttgcctacc tcgtcctctg gtatgaggtc ccctcctggg ccctcaccac ctgcttctcc
     1561 atgatatttt tgatggtatc cgtcggcgcc ctcttcgtcg gggtctgctt ctcggagagc
     1621 tggctatcgg gggcggtgaa cgtcgtcatc gaccaggtcg agtcgctctt caaaagaagc
     1681 gtcaacagga gcttctactg ctccatcacc cgctcctttc agcagagcat gctcctcctc
     1741 ctccgaaata ggaacaggcc cctcgtcatc agctccttct gggccttctc cggctggcta
     1801 tccctcaacc tcgtcgcctt cgccaccttc aggtccctgg gggtggaggt ccccatcttc
     1861 gccatctttg cggtctactc ggtgatcatg gtcttccaga acctcccgct cattctgcca
     1921 ggaggcgtcg ggatcgtcga gatcctcatg accgccctct tcaccgccgt gggggtatcg
     1981 ctccacgatg cggcggcggt cacgatcctg gcgagggtcg cccagctctg gttccacacc
     2041 ttcctggggg gtatatgcac cgtttacctc ttgaggaagg tcgacgagct ggagaagagg
     2101 cgggggtcgt cgagggtcca agctccaggt ccctgagggc gaggggggcc ttccggcggg
     2161 atcgccctcg gataaaaggg gaagaggggt cgatatccac cttattccgg gagctggcta
     2221 cctctcttgc cggctattct cctctttcgt aagatccccg ggtcgaagaa gaaggtcgat
     2281 atcggaggga gaaaagggta ctatgaaaga gggtgttggg ggagggatcc tcccgcccct
     2341 cttcagccct tgatgtcggc gtggtactcc tggagggcct tgaccccgga cctccccctc
     2401 ctcaaggcgg cgatcccctt ggcggccgcc gaggcggcgg cgggggtggt gatatagggg
     2461 agcttgtact tgatcgccgc cttccggatg taggagtcgt cgtactggct cgccttccca
     2521 atgggggtgt tgatgacgag ctggatctcg ccgttcttga tggcgtcgtc gatgttcggc
     2581 ctaccctcgg tgagcttcag gatcagctcc gactctatcc cgttctcggc caggtactcc
     2641 tgggtacccc gggttgcccg gatggcaaag cccatccggg agaactcctt cgccgcctcc
     2701 aggaccttgg gcctgtcctt ggaggcgacg gtgatcagga ccgtcccctc ctgggggagg
     2761 gtctgcctcg ccgcctcctc cgccttgtag aaggccatcc cgaaggagtc ggccatgccg
     2821 aggacctcgc ccgtcgacct catctcaggc cccaggaggg ggtccacctc cgggaacatg
     2881 ttgaagggga agaccgcctc cttgacgccg aagtggggga cccccctctc ctggaggccg
     2941 atctcggcga gccccctccc catcatcagc tccgtggcga tccgggccat ggagacgttg
     3001 cagaccttgg agacgagggg gacagtccgg gaggcccggg ggttcgcctc caggacatag
     3061 acgacgtctt tagcgatggc gtactgcatg ttcatcaggc cgacgacgcc gagctctttt
     3121 gcgatcttgc aggtgtactc tttgatcgtc tcgatctgtc ttttggggat cgtcaccggg
     3181 ggtatcacac aagcggagtc gccggagtgg acccccgcct gctcgatgtg ctccatcacc
     3241 gccgggacga aggtggaggt gccgtcggag agggcgtcgg cttccgcctc cagggcgttc
     3301 tccaggaatc ggtcgatgag gatcggccgc tccggggtca cgtcaacggc ggccgccacg
     3361 tacctctcca gcatctcttc gtcgtagatc acctccatcc cccggcctcc gaggacgtag
     3421 gatggccgga ccatcagggg gtagccgatc cgggccgcca cctccagggc ttcatcgagg
     3481 ttgctcgcca tccccgactc aggcatgggg atctccatct tctccatcat catcctgaac
     3541 ctatcccggt cctcggcgag gtcgatggtg tcgacggagg tccctatgac cctcgccccc
     3601 gcctcctcca gctcccgtgc gatgtttaag ggggtctggc cgccgaactg gactatcatc
     3661 ccctggggct cctcccgctc gtagatgctg atgacgtcct cgacggtgag gggctcaaag
     3721 tagagcttgt cggaggtgtc gt
//
LOCUS       DASM01000160           16343 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_21687, whole genome shotgun sequence.
ACCESSION   DASM01000160 DASM01000000
VERSION     DASM01000160.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 16343)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16343)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16343
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_21687"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         3516 a         4781 c         4930 g         3116 t
ORIGIN      
        1 aggacctccc ggcgggccac ctccgggtcc caggtcccgc cccagccggt ggagccgacc
       61 tcgccgccgt gctcgtgcat ctccttcacg gaggcgtggt cggggatctc ggccctggag
      121 tcgaggtaga aggtgtcctg catctcccgg gccggatggt cctggggctg gaagagggcg
      181 tcgaagttcc agaagctcga ctggacgacc tcccccttga tctcggaaaa gcccatctcc
      241 agcatgatct ccctcatcct cgataggagc cgctggtagg ggtggacctt cccagggtag
      301 atccgctccg cggggagggc gacgtcgtag ggtctgatgt agaatcgttc tcccttatat
      361 tgaacgtaag agtcttcgtc ccactttccg gcccggatca tgtctggagt aatttggcca
      421 atccctctga caacgtttgc tgtcacttca actgttaaag ttctctcgaa tacggacctt
      481 ccggcatcgg tgatctctaa agaccaggac ttctgggccc tcgactccac aagccccctg
      541 ctcttcaaga gcttcagggc atctgcatcc agggccgacc cgctcgtctc cccctggaac
      601 gaaagtgccg ccaggatctc ctcgtccttt cctggggggg catcccctgc gggcttgatc
      661 ttgccagcct cgacggccgc ccagcccttc ttcttgagcc agcctatccc caccttcagg
      721 gctggatctt cgatttccga tatggggacc cccccggccc cggcgatctc gaggatcctc
      781 ctctcgggaa gcccgatctc ggcgtaccga gcgccctctt cggtgaggga gtagacctct
      841 tccacctttt tatcgaggct gacgaggccc aacccggcga gcccctccgc cgaggagatg
      901 acggagacct ccttgacgcc gagcctctca gctatctctt ttggatcgca gagcccctcc
      961 agaacttctt tcaaaacctg cctatccctg gtagataggg cagagagctt gtcctcttct
     1021 gatggcatca tgatcaacct ttgcgaaatc gcgatcttca agggtaggca gatgcgaggg
     1081 accatatatt aaacctgccc gccgcggagt cgcggacctc agatttcagg agaggcgcgc
     1141 cgaggcagaa gtgcgaggag aaaagaaaac gaagaggagg ggagacggtc aaggagatgg
     1201 ataggtctcc agctcctcca gggcctccag gaccccctgg actgccatct ccatgaaccg
     1261 ctcccccttc tccctggtgg cccgggaggg gtcgcccatc actccgtttc ccatcagggg
     1321 cctcacgtcc ctcatgatca ggtacatcgg cctggggggg aagtgcttct cggggaggcc
     1381 cttcaccagg tcctcccgga tgaccagcat cctcgaggtc tcgatctccc cggcgtggcc
     1441 gtccccgggg gtctcgacct ccctcggatc gatcaggtcg aagtcggaga cgaggctcac
     1501 ccggatatcc cttaatgcga ccgccttctc cgccgcctcc ttgagggcgg agaggtgctg
     1561 gccgccggcg tggcccgtca ggatgaggac cctctcgaag ccggagtcgt gaaaggctgt
     1621 gaggaggtcg gagacgaggg ccctcagggc ccccggactg atggagatcg tccccggaaa
     1681 gccccgggtc gaccggcaga ccccgtagtg gaccggcggc gcgacgaaga cagctcgtct
     1741 ccgggcgacc tcctttgcca cctccagggc ctggatcgta tcggtgaagg tggggaggtg
     1801 ggggccatgc tcctccgtag cccccaccgg gaggatcaga gtccgggtca cttctagggc
     1861 tgcctcgatc tcggtccagg tcatctcctc gaggagcatc ataggctgat cgaactcccg
     1921 gcgatcaccg ccccctcggg gacccggacc tcgtactgga cgttgccgac ccgcagggtg
     1981 taggggccga caggccccac gtcgaagttg gtggaccagc tctctggacc ctccgtcgag
     2041 taggggacga cgagggagaa ctggccgtcg gatccggcgg cagtggactt ctgatatacg
     2101 aagctcctgc ccctgcccgt cgatatcgga acggagatga ccacatccgt cccgggggag
     2161 gccgtcccgg tgatcgtggc ccccggaacg tgctcgaata ccttgacgta cttctggccg
     2221 ctggaggtga tcgagctcgg ggactcgtgg acgagcctgt aatggctcag ggcctccagg
     2281 ggaactgagg tcatgatcgg gctctgggat accacctcgg ccgccgcata gccccgcccc
     2341 aggctctcgt tgacgctatc gaggagctcc tggtagtttc gagacctctt ctggggctcc
     2401 accgataccc cgccctgacc gtcggtgcgg taggcgatcg tccacccctc ctggaccctc
     2461 gcctccgtcc cgtcgaagaa gtggagccgg gcgaccatcg aactgaagta gggaggccga
     2521 tagatcatga tcggctggcc gtcctgccag tagctgaaat ggtatgtgcc gggatttatg
     2581 ttgctccagg cggccatggc gtggaacttc cccatcgcca tctccacgtc ggtgacgacg
     2641 tacctgatgt tggagtaggg ggacctgttg acgtcgaggt tagccgccac cgcctccgcc
     2701 tccgactcgt tctgggccag gaagaagggg gacgaacccg caaccccggt ctctatgctc
     2761 cctatcccct gctggaaggg gttggagttc gcaagccttt cgcccaccgc ctcgatggcg
     2821 tggccgtagt cccaccagga catggtccca taggcggagt cgggataagg gaaccgttcg
     2881 cccttgggcg gccgctcgta tatggtgtag atccccatct ccggcgaggg ggtgttatcc
     2941 cggagccagt acatcgattc gtaccagtcg gagttcatcg gtcctacctg gttttgcgag
     3001 tagaagcttc ctatcttaac cccctggact gacccaaaca tcgagggata gacgaagagg
     3061 accaccagga aggcgacgac cacaaagttg atgatggccc ggttctgatc cggagcctct
     3121 ttggacgacc ccctcgccat cctgaccaag gcgtcctcga cctccaaaaa ccgggtcttc
     3181 tcgaggagga ccgatcccag gtaaccggcg aggaaggcga cgttgacggc gtagtagtag
     3241 gcgaacctgt tctgggcgaa ggtgagggcg aggatgatca ccgtccatgc gaggatcagc
     3301 atatgcccat tccggctgtc gtcccgccga tacctcagga ggatgagggg cagggcgatg
     3361 agggtcagcc agaagggcga caggatcggg aagatccctg ggaagttccg ctggatggag
     3421 cccctctcca gtatcctgga agcctctgat atggtggctc ctcccccggt cctgggctgg
     3481 aatatcttca ggcctgccat gagggacccc gagaactgag gaaccgccag catcaggacg
     3541 acaaagacga cgactatcga accgatgact atcaggggga agtagcgcct atccatcttc
     3601 cgctggaaga gctgggccga tatgaaggat aaaaagagca tgtaaaggat acctacaatc
     3661 agcatcgtcg gctggaacag ggagtagtaa aaggcgctaa agccgttctc ggccctgacg
     3721 aagggcagga cgaggaggag ggcgactaag aaggcgatcg atccagcgat ccccaggtac
     3781 cccgcgtccc tccccctcat gtggtcgact atgctctgga ctgtgacgaa ggcgaggatt
     3841 atccccacga agaggtggcc cgatgaccag gcgtctatgt agaaccccag gaagaggccc
     3901 gccagcaggc agtaggcaag ggtcctcttc agagcggtta gatccatcct ccggatggcg
     3961 gcaaaggtga gcttgccggc gcccgaccgg aaggcgagga tgaagaagag catcgccgtg
     4021 gtgctgagga ggacctctga ggcgtgatgg tctgcaaacc ctatcaacga ccgggagagg
     4081 aactggcccg gcaggacggc aatcatgacc gcggccatga accccgccgt cctccctccc
     4141 agttcccggc ctatgaagta gactacaaag accaggaggg ccccgaagac ggccggcaac
     4201 accgccccca ccacctcgac ggtgtgcatg ctgggggtgc cgagatccgc gatgtaagag
     4261 aggatcgcga tcgcccagct gttgaagggg ccaaaggaga tctccgtccc ccttgggaag
     4321 aaggtcatgg gatcgaacca cggccgctgg aggttgagga cggtgctctt ggcgagcatg
     4381 atgtgataag atccatcgtt gccgtcgaag aggacggaat ctccgacaag gacgcgcgac
     4441 atgggtttgt agacccgcag gtagagcgag aagaggaaga cgactcccag gccgacgtag
     4501 atgtaagggt ctgggatccg ttccaacggc ctcctcttcg gagccggctg atccttcccc
     4561 tccgtgcccg cttttttctc cttcaccgat ttcttggtca tcgtgccgga agaaccattt
     4621 tcaatccaga taaacatttt gaagcgggat ttgaggaggc tcggccagga cggggatcct
     4681 cccctccacc ggcccatcgg gaggccaggg tcgggaccga tcggaggctg agaagatcac
     4741 aggcccgggg cagggatcta cccctcgtca gatggggccg gaggtcacca tccaccatag
     4801 acgatgaagg agctggtgat cccgaagatc ttctcttccc ggatcgttcc gccggaaaat
     4861 agctcctcca cctcccgcct cttgaggagc cggacggagt ttgcggccgc cagggcctcc
     4921 tctctatcgg ggatcttcgg gtaccagccg aggctgaagc gctggatcaa aaagaccttc
     4981 agggcgagag ggaagaactg gaagcccggg aagaggaagt gaggctccag ggggaagctc
     5041 cagttcggcg tctggacgaa gtacctcctc ccgacgcgca tcaactcccg ggccatccgg
     5101 cgctgctcag atggcccccc cacatgctcg atgacggagt tggagaaggc gacgtcgaag
     5161 gctccatcag cgaagcccct catatccctc gcatccccgg cgacgaggcg gatgttagag
     5221 tggcggctat ccgtcctgga gaggttgagg atggttatct cgtagtccgg atcgtcggcg
     5281 aagcccatat tctcccagaa gagctctgtc ccgccgacgt ctatgagctt caagggcttt
     5341 gggaggggtg cggccagatc cctgaagagg gcgatcctct gccgcctcac cttcatgacc
     5401 gccgaatcgt cccggctgac gtcaaacatt tttttaagat tttttcccat catgccccat
     5461 acccccgatc gttcgtcgcc aaatcgtccg gatccctgcg agagtgtcgg ctcgattgtc
     5521 gatggttaaa gcttaaaacc tttggggcgt ctatatgaag atcaggagga atcggatgaa
     5581 gaagatcaac gatataaagg gccttggcgg gatgctcaac ggatttcgag acctggtgaa
     5641 ggaggacgag agcataacct tcgtcggaac cccgggcttc tgcacgccct tcgccacctt
     5701 cctcggcttt cccgtgcggg agaagaggct ggccttcgtc cccggcctga aggtggaaga
     5761 gacgagagcc ctggtggcga cggggtacgg gatggagctc ggcaaagccg ccaaccccga
     5821 cgccgacgtg gtggtgatcc tgggagggat ggcgatgccg aagatcgggg tctccatcga
     5881 ggagatggca gccctcctgg gaaagatcca gcacaagaag ctgatagggg tctgtttcat
     5941 ggggatcctc gagcaggcgg gatggtgcgg gactccggcc ctcggcttcg attacgtcat
     6001 gaacacgaac ctcagcgggg atatctccgg ggtatagcca gaggggacgc aatagagggg
     6061 aggataccag acaaaagggc gggatttaag gtcctaaaat acgaaaacgg atttctggtg
     6121 ggcccgacgc gattcgaacg cgtgacctca cggttatcag ccgtgcgctc caaccatgct
     6181 aagctacggg cccggcagct tttctgattg agccacccct taataacagt ttccctagag
     6241 gtgctcccgg aagacggggg cataaggcct ccccatctcc ttcttctgac gatcggctat
     6301 gcgccggaga gaccttccga ggatcccccg gtccggcgat gctcttccaa aggatgtaaa
     6361 cccgtccgct gaccacctcc ggcgctgcgc ccttcgacag agttaaatct tcaagggatg
     6421 aggatctgag ggtgatgatg agatggccga cgctgaaatt gagaagagag aggaactttc
     6481 tgggctctac gacctggcga tccccatagg gatgcccctc agcgtgatcc aggatctagt
     6541 ggacagattc gagctggagc cggtgaggcg gaacgccaag gtcggccttc tggacggcga
     6601 gtccgaggag agggagatcc tcgtcctccg aggagacttc gatacggtga aggcggcgga
     6661 aaggtacatg ttcgaggccc tcgaccgcag gctggctaaa tgggagagga acgagcggtc
     6721 cgatcggtac cgggatatgt acgacagaaa cgccgatgag agaaggcgga tggtgaagga
     6781 gaggattgcg gagaagaaag aggagctcag cttctgaccc ctcccggcag ggtcagagga
     6841 tccccatcat cttgtgggcc tggggtatga cgaccacccc ggagaggacc tccgctgccg
     6901 cctcgaatag ctctgtcagc ttctccggag acggcctccc gtcccccgag gcgggctgga
     6961 ggacgaccac ggcgtccagc cccgccagcc tgggaactag gccctctatc tccgccctag
     7021 gggtcgaggc gaggaccacc accttggcga tggtgaagac cccccggcgg gaggcggcct
     7081 cgatgcaggc cagctcgttc tcgaccagct cctccgatcc cccccgggag aacgcgcttg
     7141 gaagctttat gtcgacggcc gcaatatcga tccaagggat catcttcgag aaccggcggt
     7201 gagagaagcc gttcgtctct agatagacgg gcattccctc gccgacgcaa gccctcgcca
     7261 gcgcctcgac gaactcgggc tggcagaggg gctcgccgcc ggttatcgag acgctatgag
     7321 tccctctgcc ccagagagcc cggacctcct ccatcgtcca ccccaaggat acgggatttt
     7381 cagcccgtcg gcgaaggccg ggggcgatca cctcgcagag ctccaccttg gggcggaact
     7441 tcgccgagtc gcagtaggcg caagcgaggt tgcatcccgc cgtccggacg aagacctgcc
     7501 tcctccctaa gagcggtccc tccccctgga aggaggagaa gatctccccc aagaatccag
     7561 agtcagaggt actcggtggg gtcaggaacg cccgcctcca gaaagccgaa cctacggtgc
     7621 tgacacgcct cgcactcccc gcagtggagg gccccatcca ggtagcagga ccaggtcagg
     7681 gccatcggcg cccccagatc cgacccgagc tttacgatct cgggcttctg gaggtggacg
     7741 aggggcgcct cgaccctcgg ggggatgctg aaggaggcga cggccttctc caggaggagg
     7801 ttgaacctat cgacaaactc cggcctgttg tcggggtacg acctcccctc ctcggcgttg
     7861 aagccgacgt agaccctctc agcccccacc gactccgcat acgccgccgc catggacccg
     7921 aagacgaggt tccggcagta tacccaggtg gagggggtga ccctggggcc ccggatatcc
     7981 acctcgctcc ccatggggat gggggccccg gccgtcgtca ggggtgtcat cttccggaag
     8041 agatccgtct tcgcgaaccg tacctccacc gcccccaggg cccccgccac ctccgcgatc
     8101 gccctcttct ccctctcctc cgccttctgg ccgtagagga cgtggaagat ggagatctca
     8161 aagccgtcgt ccagggcctt cgtcgccgcc accgtcgagt cgagcccccc ggagcagagg
     8221 cagaccgcct tcggcgtcat ccggtcatca cccacttgcc cttcccctcc cagagcttca
     8281 ccgagacgag ggcggcgttg gagccaagga gctgctcctc caggccggcc ctgatccgct
     8341 ctgcgataca ctcggcggtg gggttgtcga ggatctcatt gaggtcgcgg tgatccagct
     8401 ccaccaggac cgatctgagg gactccttca gatcgtaaaa gtccatcaaa aagcctgtct
     8461 tttcgtcgac cggcccctcg actaccacct ccacctcgta ggtgtggccg tggacgcggg
     8521 cgcatttgcc gtcgtatccc ggaaggcggt gggcggcgtc gaactccagg gagaggccca
     8581 tcctcattct tccccatcct tcagccgccc ctggcggcgg tctatctcgt cgagctggtc
     8641 gagcatcttc ctcagcgccg tccggacggc gtcggagaag gtgacgaact tcccgtcgtc
     8701 gccgacgtgc cggtttacat cgtcgatgat gtgttggggt atctccaccg atatcttggg
     8761 caccataatc ctccagtatt cctgggagaa tattcgagga ttattaaacc tttcccgatc
     8821 agggctccgc cttctccagg atctccggcg gcgcgccggc cagggccacc agcgcctcag
     8881 cctccatgaa gtggagcctc gccgggacga cgaggatctg gaggggaccg cccagatccc
     8941 gatccgcgag cttcggcagc cagtcggcga cgacgacggc gtcctcggag cccgccctgg
     9001 ccaccccgac gcccagggag gagttgaacc gatcgcctcc ggccgcctcc tccatctcca
     9061 ggaggagggc cgccccctcc ccggcggtca tgtacctctc cggctggatg tcgaggaaga
     9121 ggagggtgtg aaggtccctc gcgaggttat cgacgactac gtcccggggg ctcatggcga
     9181 cgatcctcct cccccccgcc acgtaagggt agggtatcga ggtggacctt ccgaaccgat
     9241 agttctgaag ccccgtcatc ccggatacgg cggtgacgat ggaggaggag tggactatcc
     9301 tcgtctctat ccccatcctg agggcccgga gacggaggtc caggtgggtg gtcgagatca
     9361 tggggtctcc ccccaccagg aaggctacct tttcctcctt cgcccggccc agccagccct
     9421 cggggctcac ctccacctcc tcccgggtca ggactttgac ctctctgccg tatagctggg
     9481 tgagcttttc aggagttgcg cccatgagcc gagaggtgta aaactccgca tagactaggt
     9541 cggagtctct gatcgtctgc aggcccttga ccgagacgtc cctctcgtcg tagagcccca
     9601 ggccaacgaa agtaagcatg aggtcctcct cagcaggagg gggcttaaag gcctcgcccg
     9661 aggaaaaacg gcgagcaaag gctaatatgc gttagcctcc gaataaattc ccatgatcgg
     9721 caggacgacc caggttgtgg attgccgaga gagcatgggg cttgccaagg gcggcggcct
     9781 ggcccagcgc ggaaccctct ccgaggcctc cagaccagat gtgatcgcca tcgccatgtc
     9841 cccggggcgg aggcacataa ccaagcccgt ctgtgagatc acctacggtc tgcggcggga
     9901 ggggatccag acgagcgtcc tcgtcctgga ggcgggggcg ggggttcccg acagcttccc
     9961 atccgcctcc aaggggttcg gccccacctt cggcctctcc cccaaggaga tcgagcagat
    10021 cgagaggcac aagatcgccg tcatacacct cgggaatatc aggtctcacg tcgtctacaa
    10081 ggcgaaggag gtcctggccc tggcggagat cccagcggtg atcgtatgcc agagccccgt
    10141 cgacttcgag gacttcgcga aggtgggggt gaagacgagg ctggtgatgc ccccgaggaa
    10201 caaggtcgag accctgggga ccgtcgtcga gattgtctac ggcgtcaccc ggggcgccac
    10261 gtgccagagg gcgaagctga accagttagc aaaggtcctc aaccaccacc tagcccggat
    10321 ggagagggag gagaggagga ggagggagga ggaggagatg cccgaggcgg agaggaaggc
    10381 gagggcgaag aagaaggcgg aagaggctaa ggcggaaggc gggatgagca ccttcttcgt
    10441 atgaggggag ggttcagagg gtgaaagctg cgacgctgcc gagggtcacg atatacaacc
    10501 tcgcgagcct tgatggccgg gtggacaggg tcgcagactc gccggaggcg atgctcctct
    10561 actacgagct atcgtaccac tggaaggctg acgccatcct cgcggggagc gagactatct
    10621 tcgttctggg agggcacgag gaggaagaga tggcgaagga ggagccccct ccaccccgga
    10681 aggagccgcc tccggggacg gaaggcctga tctatgagcc tcggccgctg ctcgtcgtcc
    10741 ccgacagccg ggggcggatc cataactgga ggctcctcca gcaggagccg tggtggcgga
    10801 agatagtggt cctctgttca aaggctacgc caccatccta cctggattat ctgggaaaga
    10861 ggcacctgga gtacctcatc gtcggcgaag atcatgtcga cctccggcag gccctcgaag
    10921 agctgaaggc ccggtacggc gcgagatcgg tgcggacgga ctgtggagga aggctgaacg
    10981 gcgtcctcct ccgggcgggc ctggtcgacg aagtgagcgt cctcatcagc cccaccctgg
    11041 taggggggcg gagagggacg agcctcttcg acgaaggtga gatagaatcc ggcggtgagt
    11101 tcgtccatct caggttgacc catatggaga gggtgagggg cggcttcgta tggctccgtt
    11161 atgaagtggc agggcgagag gctgcgcctg aatagaacgc tatagtccaa ggactggaga
    11221 cgcgctaggc gagaggctcc gagggccgga gaatttggga gagcaggatg aagcttgtta
    11281 tattgctggg agacgggatg gcggaccatc ccatcgaggc gctggaggga aagacgcctc
    11341 tggaggcggc agagaccccc aacatggaca ggatagcccg agaagggcgg gggggccttg
    11401 ctcgaaacgt gccgccgggg atgccgccgg gaagcgacgt cgcaaacctc tcggtgatgg
    11461 gctacgaccc ggagaggtac tacaccggcc gcgcccccct ggaggcggcg gcgatgggcg
    11521 tctctctcgg tccagaagac gtcgccttcc ggtgcaacct ggtcaacgtc gacctcgatc
    11581 gtggggtcat ggtcgactac agcgcgggcc acatctcctc ggaggagggg cgggagctta
    11641 tcgaagccct caagaaggcc cgccccgaag ccagcctcta tcccggcgta agctatcgaa
    11701 acctcctcgt ccagggcgac ggcgaaggcc tccaggcggt ctgcaccccg ccccacgaca
    11761 tctccggcca gccaatcgat ggatacctgc cgaagggaga cgacgcagcc gccttgcgcg
    11821 agattatgct ggcgtcggtc ccgatcctcg ctgaacaccc ggtcaacctc cgccgcgcga
    11881 aagagggcaa gcggaccgcc aacatgatct ggctctgggg gcaggggaag gccccctcca
    11941 tgccgaagtt ccgcgagctg tggggcatag agggcgccgt catctccgcc gtcgacctcc
    12001 taaaggggat gggggtctac gccggcctcg aggtaatcaa cgtccccgga gccaccggag
    12061 tcctcgacac caactacgag gggaaggtcg ccgcctgcct cgaggccctc gagcgggtgg
    12121 acttcgtcta cctccacgtc gaggcccccg acgagatgag ccacgatggc aagctcgaag
    12181 agaagatcga ggcgatccag cgcttcgacg cccgggtcgt cggaccggtc ctcgatggcc
    12241 ttgaacgatc ggggcaccag tggcgcgtcg ccgtcctgcc cgaccacccc acccccatct
    12301 cgataaggac ccacaccgcc gagcccgtcc ccttcgccat gatcggctcg gggatcgagg
    12361 ccgacgggat ggaggcgttt tccgagcggg agggggctcg gggcgggtac gggacggtcg
    12421 aggggtggcg gctgatgggg atgatggtgg agacgtagaa caaacatgtg cttttaagta
    12481 caagtaatgt aataatagca tgttttaata taggagggat tttaataagc gaggtacata
    12541 tccgcagaat tagtttatct ctagaaaaga agtataaaaa tttaattgat atcgaagact
    12601 gcaaaggaaa atctgatgag ataaaggata atgcatttct tagtcgaagc ttggcagcgt
    12661 atacactcac tcaagttgcg aaaattgaag acgagatagc tgcaagatgt gttgttgact
    12721 catttgatga taatggaata gatgcaattt attttgacag agataattat atagtttatg
    12781 tcgtccaatc gaaatggaga aaatctggcg aaaaaagccc aactacgagt gagattaaaa
    12841 aatctatcga tggctttgga gacttgctaa acgctaaatt tgatcgtttt aatgataaag
    12901 tcgcaaaaat ggaagatgaa atttttgagg ctctttacga ctcaaaagta agatttgttc
    12961 ttgttgtcgt atatactggc cgacagcccc tttcacgcca ttcgatacgc catctagatg
    13021 atatgttaca aagatataat gagggatatg aaatgctatc atataaaata tatgggttga
    13081 aggaattaaa taaggccatt tcggaacagg tccaaggtga tcgaataaat ctaataatcc
    13141 tcctaaaaca ttggggtaga attgatgagc catttgtggc gtattatggt caagttgaag
    13201 cttcagatat cgcttcttgg tgggataata attctaataa tctatttgcc ccgaatttga
    13261 gaatgtttct tggaagtaca gaaattaatg attcgatcat cgcaacatta gataagagcc
    13321 ccgaaaaatt ctggtacttt aataatggta tcacgatatt atgttcgcgg atattaaaaa
    13381 aaccgatcgg tggcgataat aaggatagcg gcgtatttga atgtgagggg gtaagtatag
    13441 ttaatggcgc ccaaactgtt ggtagtatcg ccaaatctcg ttcctcaaat cctcaaaata
    13501 ttcaacgagc tcgtgtatta gtcagaataa tatctctgga aaattgtcct gaaggatttg
    13561 ctacagaagt tacccgtgca acaaatacgc aaaacaaaat tcaacgaaga gattttgtat
    13621 ctctcgatcc tgaacagaga cggttgcagg aagatctgct gttacttcat agcaaaaaat
    13681 acacatataa aactggcgag aaaacaccat caactgaaga agggtgtgat gtcgatgaag
    13741 ccaccgtggc tttagcatgt gcaaatgatg atttgggact agcggttcaa gcaaagaggg
    13801 aaatcggtaa gctctgggag gacatcaata agccaccata taaatcactc tttaattcag
    13861 atttatcagc cactcgtcta tggagagcta tagaaattag ccgcattgta gaaaatgaat
    13921 tgaaaaagtc agaagtggac cgacagggac gagaacgcct catatcagtc catggaaatc
    13981 gattcatact tcaacaagta tttaaaaaat atcataattt tgataaaatt cctgatatcg
    14041 attttgaaca catgaaggac aaggcaccca aagaaactct tcgtatattg gatgatctaa
    14101 ttacagcagt tgaagataag cttccaaatt catatcctgc aaatatattt aaaaatagaa
    14161 gtaagtgctt agaacttgag gcgtatttaa agagacttga gggctgatat aaaaagctct
    14221 taaaaggcaa gctttaatta cttatccatt ttattgcttc atagactcga gaattttcca
    14281 ctctatctta aatcgcctat acatagccag cccacagttt tcgcgccgcc cgccgcctcc
    14341 gctccagcgg cgcgatgccc cgcgcaccct ccgggcggga cggctccgcc cccacgagct
    14401 tcgcccggct gaaatccgat aagaatcatc gtatgaaaaa taagttatgc catgaaaata
    14461 atcgtattat ggtgaaatat ggccgctcga agaattacga tcgacgtgcc caaaaaggtg
    14521 ctcttatccg agaagaccga cgagaaggcc ttcgggcgca agctgcggat tttggcctca
    14581 gtcaagctct atgagctagg tcggctctcc tcaggacgag ccgcggagct tgccggtatg
    14641 ccgcgattcg agtttctgct gaccctcgaa cgctacaagg cgtttcccct cgaagccgag
    14701 ctgcgagacc tggagaacgc cactccttaa agacccatca ttttctagaa tccacctact
    14761 tcgtggcgct ctgaaatcgc ccggatgaag ccagcctccg tggatttctt gccccccggc
    14821 cgctcgcctt cgacccgcaa cctataagcc cttacatccc ttacagcatt tacatgggcg
    14881 aagcagccga taagaggata ccagtgaccc gggaagtctg ggaggatcta tcagacctca
    14941 agaggccggg agagaccttc gcgggccttc tggcggagat gatcgagcac gagaagaagc
    15001 ggcggctctt cctcgatatg gagaggatcg agggggaggg gaacttcgtg gagcttcgat
    15061 tttgacctac cagctgctga tcgaagagaa ggccctggag ttcctccgat cgctgcctga
    15121 gaagagcagg cggcttgtag cagatcggtg tctggctctc tccgacgatc ccttccctgg
    15181 aggcgaaagg ggcgaccggg aggcgctcca tctcgctggc cataagaagc tatatcgcct
    15241 ccatggtgga aggtcctata ccgtcttcta tcggatatat gaagacgaga aggtcgtttc
    15301 gatccttgcc atcaccacca ttgagaaggc gcacaagatg tacgggcggc tctgaattcg
    15361 gcatcttctc tggacggaac ccacctacct cccgacgctc cgagatcgac tggaggaagc
    15421 caggcctacg ggttctcgcc gcccggtcat ccacggtatc ggtcccttct ccgccgcatt
    15481 ccctcaggcg gcattaccct tcggccctcc gggcgggagg cgtcgcctgc acgggcttcg
    15541 accggctgag tcggacatga ttgtcctacg aaaattaaaa tcaaaaacgg atttcgggag
    15601 agggccggga gcgagacatt cgggtcgctc ctagccctgc cccgccactc tacttctgct
    15661 atctcaagtt accaccggag ggacctccgc gggaggctcc tccacctctg ccggaggcat
    15721 ttctacggga ggaacttcct caggaggctc ctccgcctct gggagtggca tcaggactgc
    15781 gtctattatg tggacgacgc cgttgctgca gaccacgtcc gtctggacga tcctcgcgcc
    15841 attgacaaag gccccctcgt cagtgacctc gatggtgagg ctagagcctt cgagggtctc
    15901 tatggaggtc atgttcacga catccgcggc catcagagcg ccatcagcta cgtggtaggt
    15961 caggatctcg gtcagagcat cggtgtcgtt gaggtcgatc tcacccaaca gtccgaaggc
    16021 ttcatccgtg ggggcgaaga ccgtgaaggg cccctcgcct gcaaggaagt ctgcaagacc
    16081 tgccgcatcc accgcggtga cgaggacgtt cagattgcca gccccgactg ccgtctcgac
    16141 gatgttcagg agggcgggct cagtctcagc gacgaccggc tcctcgacat cctcggcctc
    16201 agtatcattg acaaccggaa cctcgacatc ctcggcctcg gtatcattga caactggaac
    16261 ctcgacatcc tcagcctcgg tatcagctac gaccggaacc tcgacatcct cggcctcggt
    16321 atcattgacg accggaacct cga
//
LOCUS       DASM01000161            9726 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_98616, whole genome shotgun sequence.
ACCESSION   DASM01000161 DASM01000000
VERSION     DASM01000161.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9726)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_98616"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1766 a         2921 c         2902 g         2137 t
ORIGIN      
        1 cggcgggaga acgccagata gatgccgtag gtgttctttt ctccagcgcg gtttccttgg
       61 agaaggttat cggaggagcc gttgagaaat atgccgtaag agttgtttcg caggtagttt
      121 gatgctaccg tattgttgct ggacctctcg aggcttatgc cgtagaggtt atcgctgagg
      181 atgttatttt ttatcgtcga atccctggtc tcgaagaact tgataccttc gcggttcccc
      241 cacaggtcca gatctttcac agtgacgttg gtgcagttga tgcagacgac agaactcgct
      301 ccggaggacg aatccacgat ggtgttgctg gagtccacca ggtaacggat cggcctgccg
      361 tttatcaggt ttgttacgtc gacttgattg tcgaaatgag aacggttcaa tccatcaacg
      421 aggaagttgt atccgttatc gatgatcagg ttgtttccga ggacgttctt tccggatcgg
      481 tttaggctca aacccccctc attctccctg agatcgttgg aagtgagctt gttatgatcc
      541 gagccttcga gaaagagacc gaagtcgttt tggacggcat cgtttgaaag gatcaagttt
      601 tgggaagagc catcgagata gatacccttg ccgttatcgt atacaaggtt cctcgatatg
      661 gtgttgttga cggactcgaa gaggtcgatg ccgcggccgt tttggcaggc ctcattttcg
      721 ctgaccaaat ttctctctga gcggtggagg cggataccac ttgcaccact ccgatcgact
      781 tcgttgccga tgatctcgtt ctcgccgcaa tgattcagga ggatgccgaa ctcgttgtcg
      841 aagatgcggt tcatctccac ccgagagccg gtggtgttca gcaagtatat gccataagtg
      901 ttgttggcga ggatgagatc tttaacggtt atgttggtgc agtcgataca gtagacgact
      961 cctgcaccgt cggaggagtc tatcaccaga tcttctgccc gcatcaggta gatgatgggc
     1021 ttaccatcga cactattcga tcgatcgacg tcgatttcgc cctcagcatg gaagttgtac
     1081 aggttattct ccatcaggtt gtctctcagg acgcacatac cagaggaatt tatgtagatc
     1141 ccccactggc cctggctggc gttgttgcca gataggctgc atcctgcaga gttctgaatc
     1201 cgtatcccct caaattcgtt ggtctttgcc ctgtttcctt cgaggaggtt gcgaggcgaa
     1261 tctctgatga atatacctat gaggtcgtcg ttggcatcgt tgtacaatat gatgttctcg
     1321 atagagttct ctgtgagata tatgccgaac catgcgttgc tggcgttatt cttttttatg
     1381 ttgttactga tcgaattatc gacgaggtat atgcagatgt actcgctatt gctgacgtcg
     1441 ttgccaagga tgctgttttt tcccgaagag tttagatgta tcccgactcc gtttttggtt
     1501 gcgacgttct cctggacgat gttgtaatcg ctagatttgt aaagatcgat cccgacgaag
     1561 tttaagctgg cgttgttaga atcgactctg ttctcataag aagattcgag gactatgccg
     1621 taagagttgc ttacgacgat gttgccgtcg acggcgtttt cgctggaaga gttcagaaat
     1681 atcccgtttt cgttctcgat gacgctgttg gctgtaagcc tgttcgagga agaattctca
     1741 acataaatcc cgttccagct gagacttata cgattgtctt ccaccgccga attgatggtg
     1801 ttggagaggt aaaccccaaa gaagccgttg gtgaggttca gaccttttat tgtgacatta
     1861 tcgcagttga tgcagtaaac ggccccagca cctgacgaat cgtctatcac ctcgtcggag
     1921 acccctaccc tgtagtatat aggcctgccg tccaccaggt tggtggtgtc gatgtaattc
     1981 tcaccatcgg cacggaagtt gtagcggttc tccaccatga ggttactatc cataatgctt
     2041 ctgacggaac cggataggta gatcccgtag ccatttccta tggctttgtt atccttgagg
     2101 tagatgtcgt ttgacccctg aaccgagata ccatatcggt tctcttcggc gtagttatct
     2161 tggagggtga tgctatggga agaattctgg atgcgaaagc cgttttctgc gttataggag
     2221 gcgtcgttgc cctcgaggga gttggaaggg gagccatcta tcaggatccc atcaagttcg
     2281 ttgtagcccg cgtcgttttc aatgatgctg ttgttgacag aactttcaat ggatattccg
     2341 tgcatctcgt tatgatggac tacgttattg tctatgatgt tgccgctgct cgaatccata
     2401 agtcggattc caatccatct attgccgctg acgatgtttt cggatattac gctctcgtta
     2461 gaactccaga gaaagatccc gcttttgtta ttatagctga cattgttatt tatgatggaa
     2521 ttatttttgc tggagattga tacaaaaatt ccggttccat tgtttaaacg gacttcattg
     2581 ttcataacca cgttgttgtc cgagattatc ttaatccccg cctccaaatt gtggccggag
     2641 ttggtcgcca cgaagccctg aaggattatg ccgtcggcat ataggattat agaatctccg
     2701 gcaccgccag catccaccac cggcatccca cctccggtat ccactcccag caatatgagt
     2761 tgctttgtta tgttcaggtt ctccctgtag acgccgctct tgacctccaa gacgtccccg
     2821 gggctggcat cttcgatggc ttcctggagg ctttgcccag catcaacggt tgtcgtggtt
     2881 ccttgagctg atcccagaag gagacccaag acaaatccaa cggataaaca tatataaata
     2941 taaccaatat ttgagggcat tgtcattttt cattacttgt gctgaaccta taaaaggttt
     3001 ttcggatatg tcaccttcag ccgctttcgg aggcgtcttc tgtcgtgact gaccccatca
     3061 gaattgagtc tatagcggag cggagatcgc catgaacctc tcgatgatct cctccgggga
     3121 tagagggatc acggcaacct cggcgtttcc tgcctcgacc ttgacgacca ggacgcccct
     3181 cccggtgagg ccagcccgga gctcttcggg gctggcggcg aggcggacct cggtgacccc
     3241 ggccccctcg gcgatccggg ccaggtctgc ctttcgggcg gtgcatgtcg gctgggaccc
     3301 cgtcgacccg tagcagccgt tgtcgaggat gacgaggagg tagttctccg gctcctggtc
     3361 ggcgatggtg acgaggctcc ccaggttcat caggacggag ccgtcgccgt cgatggcgac
     3421 taccctccgg tccgggaggg ctagggcgag gccgaggccg atggaggagg cgaggcccat
     3481 cgaccccagc atatagaagt tggaggggcg gtcgccgacg gcgtatagct ccctggaggg
     3541 ataacctata tttcccacca gaagggcgcc ctgggattcg gcctccaggg cgatgatgga
     3601 gatcgcttcg atccttctca gattgatcgg ctcccgatgg ctcaaaagga cttctgaatg
     3661 ctggccttca tcgaaggcga tcgtttcaga tgatgcctcg ctctctcatg gcggctttca
     3721 gctccgcgag gtcggtctcg atccgctcga acctctggtt catgtaactc ttcatgtcga
     3781 acctgagccc ctgaatctcg ccgagggtcg aatcctgctt ctccagcata gcatcctgct
     3841 tctccagcat agcatcctgc ttctccagca tagcatcctg cttctccagc atagcatcct
     3901 gcttctccag cattgtgtcc atcttttcgc ccagatcgcc gaagccctct tttactgtga
     3961 ccaggatctc cttcaggatc ccgaccccct catcgaggcg atctcccagc tcttcggggt
     4021 cgatcacttt tcgaaatccg ggaaaagaac ccgtcgcctc ggagtaagcc gtctccacat
     4081 cgtcgacccg gatgagagag ttctccatct tcaacgccga tgcaaacctc ccgaggtcgg
     4141 acttctcgcc ttcggctacc acctcgaccc ggccgtcggg gaggttctgg acgaagccga
     4201 cgaggcccaa ggaccgagcg aggtcgacca ctctcgccct gtaccccacc atctggaccc
     4261 tgcctgagac ccgcgccgtc aatctgatca tcagtagaaa tattccctcc tgaaatatat
     4321 agtccttcgt cggaggcatc cgtcgtctcc ggggttcagg ggatcggctt ccagaagccg
     4381 atctccagca gcaccgccgc cggggtcctc ctcgcaagag cgatctccca ggcgagggca
     4441 actttctcct cggcctcggt gagggtgggg atgaaactcg ggatctccat ggcgtcgagg
     4501 agcttcggcg tcagccgtcc catggggacc tggccggata tcgtctcccc cactccgccc
     4561 ctgtggctga cgatcaggag gagcgggacc ccgtaaaggc cgttgaggga agcgagggcg
     4621 ttgatgctgt ttccgagccc cgagttctgc atcaggaggg ctggcgtttt accgcccatg
     4681 tatgcaccgg cagcgatccc gaccccctcc tcctcccggg tgacggggac gtgcctgatc
     4741 tcggggtcgg tgtccacgag gtcgaggagc ccctggagat ggacgcaagg gacgctggcg
     4801 acgaagtcga tcccggcccc cttcatcccc cggtagaccg cctccgaggg ttggaggtat
     4861 ctcatcggaa gatatacggg gcttttatgg gacttaaagg ttgatccttc cgggggggat
     4921 caggtcctcg cggcggccgg ctccccccgg ggaaaaaagg gcggccttca ggccccgagg
     4981 ggccctcaga cctttaggcc aaacttctgc tggaactgat ccttgatggc ggcgagctcc
     5041 gcctgggtct tctccacgct cgtcccggtg gcgttctgga cgggctccac cggcgccgtc
     5101 gcaccgacgg cctcgcttgg agccacctcc ggctccgtcg tcgccgccgt ctcgctcggt
     5161 gcggcggtct cgttggtggc cacttcaggg gcgggggtct cggctgccgc ctcagggacg
     5221 gccgatccgg cccctaaagc ggcttcgcag tacccgggag agaggagggt aacgttctcg
     5281 tttacggtga tctccaggga accgaggagg tactccacca ccgtctcggt gacgttctca
     5341 tcgatgaatg cgatcgatac ggtctgattc gacggctgat gggcgacgag gacgtagttt
     5401 ccgatctgtc cccagatggc gggccggtcg gtgatgttca ggggcgcggg gctgtatatg
     5461 gtctcattga gggtggggtc gaagacctcg atcagatcct tcagtccctc ggcatccagc
     5521 tgtgcctcgg ggggctggca ggggtagagg aggtataaaa ataccctgct ctcgttgagg
     5581 aggagggagg ccaccaggta acgcccctcg gtgtagttct tgtcgttcaa agattcagcc
     5641 tccaccgcca catctgttgg gacgatgaat cccagctccg cgaaatcgtt caaaggcgca
     5701 acgaaggggg tcaggcttat cgcctgggct cccgccggag tccaggcaag agacgacaac
     5761 agaaggcatc caattaagat cttcagcccg ctgcgggccg acatgctggg gataacggag
     5821 agctccattc aattcacctc ataaccatct tcatctcagc cgtaccggct tcgacctcga
     5881 tccgggctcc gccgactccg cctcccacct ccccccaaag gtcaggagga cggtctcttc
     5941 ggaggttccg caggaccact ccggcttgag gggaggaagg agcgccatcg gtggcatcca
     6001 aaggcacccg gaatttctgt atatattttt atctccgcag atcatggtat tatatctgac
     6061 ggccctggga cgggatcgcc ccgagggcag cgatggaccc aggccacctc accagacctt
     6121 cagatcgatc ccctcgatcc tgaccatcct cccctcggag tggacgatca ccccggagtg
     6181 gaccttctgc atcatgcagt cctcggggag gaacctgacc gtctcgccga ccccggggac
     6241 ccggccccgg aggacgaccc cctcgaaggc ggcgacgagg cagaccccca cctcccgggg
     6301 ggcgaagccc cggtctccga ggaggtggag gggccccacc acgtggacgg tgacgatgcc
     6361 gtgatccacc tttatcgctc ttgcgaaatg ggtgacgggg cagccgaggg ggcggtagcg
     6421 gagggcggct cccacctcca gcctcgcctc ccggtcgaag gggaagaggt cctcgcggca
     6481 ggagggctcg ccggggaggg gggccaggga gaagtccgcg gcgaggccgt cgatcacccc
     6541 ccggacctcc gccgcccccc agccatcccc cctcgggcgg tggaggagct cctcgatcgc
     6601 cgacctcgcc ccccggtgcc tctcctggcg gagagggggg cagcggtcga tgaggccctc
     6661 gtcccccgcc aggagggcct cggcgaactt ctggcagctc ctctcaccgc actcgccgca
     6721 gttgtagcca ggaagaagct tctcgatctc ctccattcca tctaccctca tcccgccccc
     6781 gcccctcgca tggcccctct cactccccgg tgtactccat gaagccgtcg aggtggcgga
     6841 ggaccccccg atggtgtctc ttcgataccc ggagctcccc ggagcagagg gtgcatatcg
     6901 ctatgggtgg gttgtgccgc aggagcatct cctcccggag gtcgggggcg tttttgatca
     6961 gctccgccag ctccgccgcc cccttgccgg agaggccgtt cgcctcgacg atcctgcaag
     7021 cggggttcgc ctccaggacc ctctcccgga agacctccct ctcggcctgg gataccatgt
     7081 cccccttcgt cgccacgacg acgtccgccg tcgtcaggag cggccccacc ttcagggggg
     7141 cgttggggcc ggaggtgacg tcgaggacgc agaccgccag ggcggagtcg gggtaggggg
     7201 cgcaccggag gcagaggcct gccgtctcgt tgaggaggac gtcggccccc tcttccttcg
     7261 cccagtccag catctcgtcg acgttgtaga tcgagtagtg gtcggggcac atgtccttgg
     7321 cgaggccgac ggcgacgggg atgccgaggc ggcgaaacct cgcgtcgtcc tcggtccaga
     7381 ggcagtcgat cttgacgacc gccggcctca cccccatctg ctgaagggac ctcgcggcgt
     7441 ggatgaggac ggaggtcttc cccgagccgg gggtcccggc caccaccacc atcttcaaag
     7501 ggcggccccc accacctcgc cccgccggat cgtctcccgc aggttcatca gccagtcgtc
     7561 gaccttcgcc aggtcctccc agaccttctg ctctccgggc gtagtctcga tcaccctcat
     7621 catcccgccg ttcttcatca cgaccctccg ggatgtcatg agggcgagca tcgggtcgtg
     7681 ggtcacgacg acgacgatct tcccctcccc ggagaggagc tccagcgcct ccctcttctt
     7741 gatcccggcg ttctcgatct cgtcgatgag gacgatcggc gaatcgctga tgaccgcgat
     7801 gtcggcggcc atcagcgccc tcgactgccc cccgctcaag atcgtcagat gctggtccgg
     7861 atggaccggc tccccggtga gggtgttggc gagctctatc acccgacctg ccacctccgg
     7921 ggacctcccc cggctcttgg cgtgcatcct cagaaaatcc cccacggtca tgtcggcgag
     7981 gaagtgcatg ttctgggaga gctgggctat cagcttcctt cgggggtcgg acctcatctc
     8041 ggccctcggc tcgcagccat cgatgaggat cctcctcccc gagggggtgt ctcctttggc
     8101 gaactgctcg atgtcggcga tgagggagct ctttcccgag cccgtcggcc cgacgatccc
     8161 tatgatctcg ccccggcgca tctcgagccg ctccaccagg tcgggctttc tctccttgtc
     8221 gaagccgccg aggatggtga gcttgcaggc cctgtcgacg gtcatcgggg tgtcctccgt
     8281 cccaggatct cttcgatctc ggccagaagg ccgtattcgg atggggatga gagggccgag
     8341 ggatcggcct cgatatcggg tttgagcgtg aacatctcca gttcctcttt ggcccgggag
     8401 actccgcccc cggtgatgtt tagcagtatg aggtcgtcct cctcgaccgt accggaggcc
     8461 gcagcccgct ggagggcggc gacggcgacc gccgccgccg gcaggatctc gatctcctcc
     8521 ccctcctcaa agagccgctg ggcgagggaa gcctcccggt tggagatggc gcagacggtg
     8581 ccagccgtct cctccagggc gtctctgacc cccccgggga cggcgtaagg gggctttcgg
     8641 ttgtagagga cgtcgtcgta catcccgctg gggcaggggc cccgcacagc tgccgagccg
     8701 gtccaggcgg cgtagagggg ggcgcagggg gagttctggg cgaggtggag ccgagggagg
     8761 gaggagccga agcggccgtc cccgatcagc cgcaaggagg cctcccaggc ggcgatcccc
     8821 ccggtcccgc tcccgacggc ctggaagtag tggtccggaa gagacctcat ggcgagggcg
     8881 gcgtcgagga ctacgacccc catcccgtcc cgccgagcga cgttcctagc ccccccctcg
     8941 ccgacaaacc cggcctctcc ggccagcctt gcggcgaggt cgatcgcctc ggtgtagtcc
     9001 cccttcaccg atatgacgca tatcgactcc gaaggaggga ccgtcgtcca gaggcggctc
     9061 tcccccgagg tggggacgac gaggacgagg gggaggccgg cggccgaggc ggcctgggcg
     9121 aaggcccggg cggtgttccc cgccgaggcg actacgaggg cctgaccgtc ttttcgctcc
     9181 atcagcctct ccaccgtcgg cggagcttcc aggtccttga aactgcaggt ggccatcctc
     9241 gcttcgcgct cgggccagta gccgctgaag ctgatataga ggcgcgagag gcccagctcc
     9301 cgggcgagga cctggctcct gaaggtgacg ggcttctctc ccgacccccg gaggggcccc
     9361 tttacgggga gccagtcgat gaacttccag agccccggca ggtcctcccg gagggtgagc
     9421 ctcttcgcga cgtactcggt tcggaggagg gagtcgtcat ttgcgcagtc gagggcgcta
     9481 gggggctgca gtcttccgca gcgggggcac ctgagcagat attcgcccat gagatcatct
     9541 cgggagagcc ggggatatag ctttttctgt tttgtccaat attttgacct taatgtgaaa
     9601 ttataataaa tctatatgta ctatgaggat gtaaaagccg ggctacaggg agaagaggcc
     9661 ccagagccgg cccgggtggg tgggttatct tgacggggtg cggagagggg cgtaaaaccc
     9721 cggtct
//
LOCUS       DASM01000162           12229 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_3130, whole genome shotgun sequence.
ACCESSION   DASM01000162 DASM01000000
VERSION     DASM01000162.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 12229)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 12229)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12229
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_3130"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2265 a         4017 c         3637 g         2306 t
ORIGIN      
        1 cccccccccg gagtaaaggc tccccgcagg agagaccccg tggaggacga ccccctccct
       61 ctcgaccaaa tcgtccccct cccaatgcct catcccgaag acgtggacct cgtgcccccc
      121 tttcgccagc ctccgggaga cctcccagac cctcctctcg gcgcctcctt tgacgtaggg
      181 gtatatgacg tcgtggacgt aggcgatctt catggcgacc ggccccccgt cttggcgggt
      241 ccgggcaggg gcatcttgnn ttcgggctcc acctctaatc ctcctccccg gagttgagga
      301 ggaggaggct gatgaagacc gccgagaaga tcgtctggac tccgatgacg acgaggatca
      361 tcgccatcac cgactcctgc atctcctgga tcgaaccgta acccgcctcc atccagcgga
      421 ggacgacgtc cagccccagg accacccccc ctccgaggaa ggccaacccc gccagaagct
      481 ccttctccag ggagtggtag ctcgtcagga tcctcgcaag ccggcccgga tcctccaccc
      541 cgtaggcttt gccgaaggcg gcgatgtaga agcccgagag gagggtctga taccccatga
      601 tgaggagcat gctccccagg atgagggagt gggccctgag gtcccgggag ggctcctgca
      661 tcagtacgaa gagggtgagg gcgaggccga ggaggagggc gacggcgccg gggccgagga
      721 ggaaggggcc gggcctgtag aggatcatga accggaggtg cctccatccg tcggtgaagg
      781 agcggagctt cgaggtcccc cgccggcgat ggtaggtgat ggggatctcc tctatcgaga
      841 gccccttgcg cgaggcctcg atcagcatct ccgaggcgaa ctccatcccc cgcgcccgga
      901 ggtggagccg gtcgagggcc tcccgggtta tcgccctcat cccggagtgg gcgtcggaga
      961 tcccggcccc gaagaggagg tttaggatcc ccgtcaggag ggggtttcct aagtaccggt
     1021 ggagggcggg catcgcccct tcctcgatcc ggcctttaag cctcgttccg atgacgaggt
     1081 cggccctgcc gctctcgagg acccttatga actccgggat ctcgagggga tcgtaggtga
     1141 gatcgccgtc catgatgacg atgtaccttc cccgggccct cgaaaacccc gcgaggtagg
     1201 cgttcccgta tccgagcttc tccggcctga ccacctccgc cccgagggac cgggcgatct
     1261 cgggggtctc gtccccggag ctgtccgcca cgatcacctc cccctcgatc cccatctcct
     1321 cgaagaccgc cctcgctcgg cggatgcact ctcctatcgt ctcctcctcg tccatcgcgg
     1381 ggatgaccag gctcaccaga ggcacaaatc aacaccgagg ttccctctct taagcttttc
     1441 cggttccata aagactttca attgcagaag gtggccacct cgggaatcga cctcgatcca
     1501 tcccccggga agacggcaga aggaaactta tatgaacggc gatggcgacc cattccccag
     1561 catggagagg gtgagatctt acgagcttct gggagtcctg gccgccctga tcctggggct
     1621 ctcgttgagg ctggctcccg caagaaacgc cctggcagga ggaggggtcc aattctacgg
     1681 ttacgactcg ttctatcacg caagacggat cttatacacc gccgagaact tccctcatac
     1741 cctctggttc gactcctacc tccaccaccc cctaggccat cccatcacct ggccccccct
     1801 cttcgaccag atggtggcgg gggcggccct cctcctcggc gggtcccccg gagcggtgga
     1861 ggcggcggca gcggccttcc cccccctcct gggaacctcg atgatcctca tcctctatct
     1921 cctcgcaaag aggctcttcg ggatgagggt ggccctcctc tccgccttcc tcctcgccat
     1981 cgactcaaag cacatcgcca ggaccctctt cggcctgccg gatcacgacc ccctggagct
     2041 cctcctcatg ctgggggtga tcctcctcct cgctcacgcc ctcaccgacc gagaccggtg
     2101 ggcttggtat ggggccgccg caggagtcat gatggccgcc gtcgcctact cctggctggg
     2161 ggctccgatc tacatgatcg gcgtcctgat ctacgccacg atccagatca ccctcgacct
     2221 ccgggagggg gcggatcctg ggtacgccat cgcccccctg gcgacagcct tcggggccgc
     2281 cctcctcctc ctcctcccct tccggaacga ggcgtggctc atcccatcct tcatcggagc
     2341 cctggggtcc ctggtggctc tcggtgtatt ttatggtctc ttcctcctct tcgcggcgag
     2401 gcggctcccg tggcaggcct tcgtccccac ggtggccttt ttgagctact tcggagcgat
     2461 cctgagcagc tccaccgagg agggccgaaa ggtcggctcg ctcctcgcgg aggggatccg
     2521 ctacttcttc ggaagcgatc tcgcgagggt gggggtggag gagacgatgc cgatattcag
     2581 agtatacgag atcgcatccc tcccggtcct ggggcttgta ttcgccctgg cggggctctg
     2641 gatcctgatc ctgaggatga gggaggtggg ctggaggagg gatcatctcc tcttcctcct
     2701 ctgggctctc ctctccaccg ccctcatgat ctcccaggcg aggttcctct tcctcttctc
     2761 cgtcattgga tcggtcctcg tctccctcct cttcttctgg gggacggaga ggatcggccc
     2821 gtcgcagctc ccccggaggg gcgatcccgg gctggccagg gccggcaccg ccgccttcct
     2881 cctcctcctc ctcctccccg ccgccgtcaa ccttccggcc gtcgccaggt accagccgga
     2941 gatctccgga gactggagcg actccctgga atgggtcggc gagaagaccc ccccgacggc
     3001 ggggttcgat aggcccgtcg aggctgcaga atacggcatc ctcagctggt gggattacgg
     3061 caactggatt ttgtacaaat caaagaggcc ggtggtggca aacaacttcc aggccggggc
     3121 gacggattcg gcccggttct tcctcgcaga gtccgaagag gaggccctgg ccatcgccga
     3181 ggcgagggac gtaaaataca tcataaccga ccagaagatg ctctacggaa agctccctgc
     3241 catcgcccga tggatcggcg aagatcctgc ggggtacgtc cagatagctg cgggtccgga
     3301 ggcggtcgtc tatgcccaca cccccaggtt tcttagaacc cttctcgcga ggctccacct
     3361 ccgggactct tccgacctcg gccacttccg gctggtctac gagtcggaga ccctccgggg
     3421 tttgaagatc cccgtcagcg aggtgaaggt cttcgagcgg gtcgccgggg cgaagatcgc
     3481 cgggactacc ccttacgatg agccggtggg ggtgatcctg gagctgacct caaaccaggg
     3541 gagaaggttc cagtacttcg cctcggcgac tcctgtcgac ggccgctacg agatcgtcgt
     3601 cccctattcg acggaaggga gggaaggggt ccattcgatc ggaccctacc tggtcggtcc
     3661 cctgaaggac gtccccgggg gcgactcgag ggaggtggag gtgagggagg cggaggtctt
     3721 ggaggggatg acgatagagg tgaacttctg accccggtcc cgaccacccc ttcagagggg
     3781 ggcttcgccc tcctggagga cccccacccg ggctccgatc tcgatccagg cccaggccca
     3841 gatcaccgcc tcaaagcccc ggatcaggtc tcctctctca aggaagtagc ggcagtcgga
     3901 gaggtaggcc tcgatgttgg cagaaaaccc ctccccctcc tccccccggg gggagaccct
     3961 ctccctcgcc tcctcggccc tcttcagcca cttctcagtc tcggccctga gatcctcctc
     4021 caggctcatg gccacctccc cagccggccg gaaccggatc gatagatccg agtttggtcg
     4081 aaatcgcccg atcgagaggc tgcaccccgc cggtccgcat ccgggggcct aatcgaggat
     4141 cctgacccca ccctcgtcag catcgaccct cagcatcgtc ttgatctggt agccctgccc
     4201 cttcagcctc tccgcccccg acccccgctc tatcaccgcg acgacgtcca ccacctgggc
     4261 gccggcggcc ttcagcgcct ccagggtggc gagggctgta cccccggtgc tgatgacgtc
     4321 gtcgacgacg atcaccctgt ccccggggga gatgccgttg aggtagagct gccccttgga
     4381 gtagcccgtg acctggctta cctcgatctc ccccggcagg ccgtacctcc tcttccggat
     4441 tatcaccagg gggatgtccg tagagaggga aagggcggtc ccgatgtgga tccccatggc
     4501 ctcgatggtg acgatcttgt cgacgtccag gtttgcggtc ctgatgatgt gggtcaccac
     4561 ctccctgatc agatcnntcg ggtggatgaa gtagttgtaa tctccgcgct tgtagatggg
     4621 cgcctccaag agggatctct tcagcctttc aagcattgcc aaatcctccg taccgtgacc
     4681 taaagagccc ggggacggag cttaaatgtt gtggctgcgg ccggtccgat ccttatcggt
     4741 ccgattccca aatccgccct caggatagat ttttaagaga tgaatgtcca atataatttt
     4801 gcggctttcc gccgcatatc aatatactgg agcgaaggga atgataaaaa aattaacgat
     4861 tttcatcgcg gcgacgatga tcttcattct atcggcctcc ctggccgcat cgcagccgac
     4921 tccggaagtt tctccggagg aggcgggctt tctgctggtg acgacccccg agtccctgac
     4981 ggcggatccc accggggaat gccaggagct ggcggggtac ctcgactgcg tctccgggaa
     5041 gaaggcggac atcgtcttcg tcttcgacac caccgggagc atgggagggg agatcgacga
     5101 gatgaagtcg atcgcgaagg agttcgccga ccgcctcacc tcctcgggga tagattacag
     5161 gctggggctg acggagtatc gggactatga cctcgactgc ggcggcgaca gcgcctgcgg
     5221 agggtctgag gacttcccgt ataagatcta caacgagggg gtcctgacgg ggaactccgc
     5281 caccttcaag gcttggatcg acggcctcgg cctcggctgg ggaggggacg agcccgaggc
     5341 ggtcctggcg gcgatgatcc acaccgtcga cgaccagcag tggcgtggag gggacgcggc
     5401 taaggtcatc gtcctgatca ccgacgcccc agcccacccc gacggcgact gctgcaacca
     5461 ggagggagag accctcgggg gcgccatctc ctacctggcc gccaacggcg tgaaggtcta
     5521 cgccatcggc cccgaggagg agtcgatcca gaagatggcc ttcgagacgg gagggaagtt
     5581 ctacctcatc aggtcgggcc tcaccctgga gccgatcctg gaggatattg cggggatcat
     5641 cagctgcacc ttcggcatcg atgggatcgt cctctgcgat gaaggggctc tggaggtcac
     5701 cgtcgagctg aaggggagag acggcaccac gataccctac cggcccggct acaccgacgc
     5761 ctggatctac gtcacctgca ctgaagagct gagcgagagg tacgacctgg tctacgaccc
     5821 cgacctcgac gcctaccacg ccgtcatcga caccgtctgc tccggagatg gtggggagat
     5881 cgaggtgacg gtatatggcc gggtctgcgg ctggagcgcc gtcgagatct tcgaggcgac
     5941 ctgcggcggg gaccagtgcc ccaagccctg cctcaacgtc acctcgtccc tgagccagga
     6001 gatctacaga tacggcgact ccatcttcta caacgtctcg gtgagaaacg atatggacga
     6061 gaagaggagg atcgccatct cctacggcta cgtcgacccg acgcccaaaa cccacgtcat
     6121 aggtaccttc gtcccggaga tataccccgg ggactcctac ggcttctccg gcgacttcaa
     6181 ggtgctggag accttctaca gcggcttata cgtcttcttc gtcacagcaa cggacgtcga
     6241 ggatcccgcc tgctctgaaa gcgatgccgt ccggttctgg gtccgggagg atgagacctg
     6301 atgggagggg gtccgaggac ccccatccca aaattttctc ctattttctc ctattttttc
     6361 tgccgcttcc ccggccctca ggccgatagc tcccttctat ccccgaccaa agagcccacc
     6421 ttctccgacg cctcgatagc cccctcccat cccggctgct tcagctctcc gaggagaggg
     6481 atctttgggc ctccaggacc tcctcgacgt ggcccttcac ccggaccctc cgccagattc
     6541 gggcgacctt gccatcagga tctacgatcg ccgtgctccg gataaccccc tcgtactcct
     6601 taccgtacat cttcttcttc ccccaggcgc cgtacacctc caggacccgg tggccggggt
     6661 cactgaggag ggtcacctcc agccggtgct tctcccggaa gctcctgtgg ctcttcggcg
     6721 agtcggggct caccccgaag acctccgccc ccagatccct aaaatccttc agcctctcgg
     6781 tgaagtctag ggcttcccgg gtgcatcctg aggtgttgtc cttgggatag aagtagagga
     6841 cgacccatct tcccgagagc tccgccaggc atacctcccg ctcatcctgg tccggtaggc
     6901 agaaccccgg cgcagcatct cctccttcga tcatcttttt ttcatcggcc atatctatac
     6961 caccagatct tctgacatcc cctctcatct aggccatccc gacgaataac ccttctcatc
     7021 tggggtccag ctggtcggag gagtcgatag agggggctca acggaagcgt aagagggctg
     7081 aaaggccccc ggcgggacca ttctccctcc ctggggggcc atcatcgaag agccctgaca
     7141 aatgttaaat agaattccga ctatccatct tatcggatgt acgaccccct cctcctccag
     7201 ataggatccg aatccctctt cagcaacgtc tgggtcctcc tctttctctt cctcttcctc
     7261 gtccccatgc tccagaggag gttcctggag cagagcagga ggaggctcct ggtcaagatg
     7321 ggcaagagga ggaggtctgt cgtggtgacc ctcatccacc ggcaggaggt cctgaccttc
     7381 ctcggcatac ccctggcgag gtacatcgac atcgacgaca gcgagcaggt cctcagggcg
     7441 ataaggacca cccccaagga cgtccccatc gacctcatcc tccacacccc tggggggctc
     7501 gccctcgccg cgacccagat cgccctagcc ctgaaggccc acccggcccg aaagacggtg
     7561 atcatccccc actacgccat gagcggcgga accctcatcg ccctcgccgt cgacgagatc
     7621 atcatggacc cccacgccgc cctcggcccc gtcgaccccc agctcggaga ccataccggc
     7681 tcctaccccg ccacctccct cgtcaagctc ctggagaaga agaagatcga tgaggtggac
     7741 gacaagacga tcgtcctcgc cgaccaggcc gagaaggccc tccggcagat ggacggcctg
     7801 gtgaggacga tcctggacgg caggtgcgat ggcgagaagg tggacctgat catcgaggag
     7861 ctcgtctcgg ggaagtacac ccacgaccac cccttcatcg ccgaggacgc gaggcggctt
     7921 ctgggggagt gcgtcaatac cgacgtcccc cccgaggtct acgacctgat gaagctctac
     7981 cggatggagg tggcgcggcg gaggcccggc gtcgagtacg tccccgtgac ggggaagtag
     8041 gccctccgat cccccggccg gagggcttct ccaccgcttt tcttttcagc cggaccggct
     8101 ctcaaaccgg atctccctca ccttgacctc ccgaggtgga ggatctgggg ggatgaatcg
     8161 agggggaggg cataccccgg ctggatcgac ccgttgcagc tccccgtccc gacagccccc
     8221 cgggagtcgt aggcgatgta gctcccggag aggaagcttc caaagacctg gaggtccagc
     8281 cggcagagga cgttctgatc gaggaagatc agatcgaggc tcacccgcgg gcctgtgacg
     8341 atgccgctcg caccgatcgt cagatatcgg gtcgaggccg ccgcccccgc cgccggtggc
     8401 gggtcctggt ggagccacca gaccatcgac tttatcccct catccctcgt cggcctcggc
     8461 cccgcctcca cggggaggaa agctggaggg gctccggcgc ccccggaggt gccccggccg
     8521 aagaccacct cgccgatctg gatgatatcg acctctgcag gctgggacct cccggtggct
     8581 ccgtccatca gcacgaggct ccagataccg gcgacggcgg ccggttgggc ctccgctgag
     8641 gtcgccgggg tggccggacc ggcgaggggg ccggaggagg ggcccgccga cccgtcctgg
     8701 agcccctggg ccgcccccgg tgagaggagg agagcggcga ggaacggaac gataaaccat
     8761 tttgatctca tattctgtaa cccccttaac ttattctcct ccaggtttaa aaatcagccg
     8821 cttctcaggg gcgtatgccg ctttaaccct cccctcagat cctaggaagc cggcggatca
     8881 gcgccgcgcg cttccccgag ggagatacct ctccgggacg gcgacgacgt acgatttatt
     8941 tccgatcagg agccagagcc cgccggaggc gaggccgacg aggaggttca tcatccccag
     9001 gacggggata ggatcgctcc agtccaggga gatcaccgtc atccctaggc tgccggcgaa
     9061 gatcccagag aagctcatga gggcggccgc cgaccccgta tcccccctct gctgctccag
     9121 catcaggttc gttgccgggg ttcggaccag gctcccagag atcgtcgccg ggaggagggc
     9181 cgccgccaat agccaggggg ctctctcccc aaagatcgag atcaagaggc cgcttccggc
     9241 gatcgccccg aaggagccgg cgatgatcga cctccgccgg agtctcctgg agacccgcat
     9301 gtagatcatg gggccggaga tcatcccgac ggcgtttgcg gcgaagtagt agctgtagac
     9361 ctgggggctg acgccgaagc cgtttatgta gatgtaggag gacgccgcta tgaaggccat
     9421 cgaggatatc cccgggaggg agaagaggag gaggagggtg gagaagccgg ggttcttcgc
     9481 cacgcggaag atccggccga gggccccgaa gagggagccc ccgctccgct cgccgatcgt
     9541 ctcctgtagc gccaggccac ccagaagggc gaggaggccg atgagggcga gggcccagaa
     9601 cgcccccctc caggagacga acttgaggag gatcgctccg aggacggggg ctgccaccgg
     9661 cgatataaga accatcgact ggaccatcgc caggatcggc tccctcctct tgctctcgta
     9721 gacgtccttg accatggcgg tggcgacggc gaaggcgccg ctcgccccga ccgcctggag
     9781 gatgcggaag gctatcagct gatgaatgtc gccggagagg gcgcagccga ggctcgccgc
     9841 cgtatatatc gagagggccg cgagaagcac ggaccttcgg ccgtacctgt cgctgagggg
     9901 gccccagaag agcgtgcccg caccgaagaa gacgaagaac aaaattaggg tcaggttcgc
     9961 caggtccgcc gagactccga agtagagggc catcccgggg agggcgggaa ggtagaggtc
    10021 ggtcgagagg gggacgaagg cgctgaggaa ggtgatgagg gcgacgagcc ccttatctcc
    10081 gaggtaccgc tgtctactgg cggaggaggg gatagtcacg tcatatcacc cccgatcgat
    10141 ctttgttgca actctctctc gcctccgatt atgacgatga aaggggccta tctgcttttg
    10201 aatcgtcaac attcagtttc atttgactct gaggctacat accgatctga gatttccacc
    10261 ttaggaatga gtactacttg gccaagcttg ccaagttttg tgaaaactag gtcgcctggt
    10321 ttcgcgagat tgctagacag gtccttgcgg acccttgact cggagacgaa gacgaaatca
    10381 tcaatgtcaa ggaaacggcc attgttgaga tttgatcctc gaatgactgg gacaccatct
    10441 tcgacatagt gactcgaagt aagcttcgag ccggatggtc caccaataaa tgagtacggc
    10501 tcatcggaag caagcagttc aagcttctgg ggcggccaac tcatgccccc acctccccga
    10561 cgtcctcccg aatggccaac gccagcgatg ccgccccggc gttgaggcgt tccagctcgg
    10621 cgtggagatg caatcgtgat tgaagaccgt tcattcagca tcctccagga gctgagagat
    10681 ctcagcaggt gagggcagct gccctttgag ctccttgggc aggcgcttga ccatgcgata
    10741 ggtcgaaacg ccgatgggct ttcgagcatc gtgcagggcg tattcgacaa ctgtgcggct
    10801 cttctccttg cagaggatga tgccgatgga cggatgttca ccctcctcgc gcacctgtcg
    10861 gtccagtgcc gtcagataaa actgcatctt gcccacgaac tccggctgaa actcgccaat
    10921 cttgagttct atggcgacga gacatttgag gcggcgatgg tagaggagca gatcgatgaa
    10981 gtactcctta tcgtccacct caagccggaa ctggctgccg acaaaggcaa acaacccacc
    11041 catggcgcga agaaagtcct cgatgcgggc gatgagggcg cgttccagct cgcgttccga
    11101 gtgctcttcg cccatctcaa taaagtcgaa ggtatattca tctctcacag caagtttagc
    11161 ctgtgtgcgc tggtcagctg tcagcgtctt atcgaagttc gtctggccga gcagggtctt
    11221 ttcgtagctc tgattttcga tctggtgaat caatacattc ttcgaccaac cgaacttccg
    11281 ggtcatccgg atgtagaact cgcgctcaag agggtctttg ctgcgagcca tgatgatcag
    11341 attcttgctc cagctaattt ctccaaccag cggttggagt ttctcgtctt cacgatattc
    11401 caggtaaaac tgccgcatat accacagatt ctggaccgag aaccccctca ctccgggaaa
    11461 ctcgttttgc aggtcatcgg ccaaccgctc gacgacggat ctgccccaac cctccctctc
    11521 ttgccgtccg gcaatcagct ttccgatgtc ccagtaaagc gcgacaagct ccctgttgac
    11581 cgccttgagg gcttcgtact gggctgaata gacgcgttcc ttcacttcag ccagaagcgc
    11641 ggcgtaatcg tcaggcaatc gctccgagag tcctttcata tccccagctc ctcgaagtta
    11701 ttcttgatcg tcgccgccag cttcaccgcc tcggcgttga ggtcctccag ctccacgtgg
    11761 atctcgcgca aggcttcctc gaaatcgaag tcctcatcct cctcttcggg cgcaacgccg
    11821 acgcagcggc cgggggtcag gctccagtcg ttggcggcga tctcggcccg gtccaccagc
    11881 ttgaccagcc cctccacgtc gcagagcttc gcctcgggga actgctcggc gagccagcgg
    11941 gcctgcttcc agaagtatcg caccttcgcc tcgccgcccc ccggcgctgg aggcctggga
    12001 cgacatgaca aatccggccc tgctcccctc gttcaggtag ctgtataagt agctgatcca
    12061 gatgtaattt ccgttggaga ccttatcctt cttgttgact ccgggaaggc cgaaaggaag
    12121 gcgaggatcg gtcttgacct tgtcggcatc gatctcgtct acgttgaagg gaggattggc
    12181 catcacgtaa tcggccttgc ccaccagctc gtgcgggtct tcgtagtag
//
LOCUS       DASM01000163            6972 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_64472, whole genome shotgun sequence.
ACCESSION   DASM01000163 DASM01000000
VERSION     DASM01000163.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_64472"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1369 a         2098 c         2280 g         1225 t
ORIGIN      
        1 gggatggagt acccccagat cgccctctgc gtgatgcgcc gctacctcgg ggatcttatc
       61 ccggaggatg acctcgtcgc gatcctggag gacgcctacg actttcccgt gccgatcgag
      121 aaggtctacg acgagaagta cgtgatgagg ctcgataagg gccccacctt ctccttcaag
      181 gacttcgccg cgaggctgat gggccggttg atgcagtact tcctaaaaga ggagggccgc
      241 tccatggtga tcctgaccgc cacctccgga gacaccggaa gcgccgtcgc ccacgccttc
      301 tacgatctgg acaacatcca ggtcgtcgtc ctcttccccg agaaggaggt gaccggaagg
      361 cagagaaagc agatgaccac cctgggaaag aatgtccacg tgatggccgt cgacggcaag
      421 ttcgacgact gccaggctat ggtgaagcag gccttcgtcg accccgactt cgcagacctc
      481 aacctctcgt ccgcaaactc catcaacatc ggccggttga ttcctcaatc gatctactac
      541 ttctacgcct ggtcccggac cgcggccccc gaccgggagg tcgtcttctc cgtcccatgc
      601 ggcaacttcg gaaacgtcat ggcggggctg atcgccctgc ggatgggcct tccggttcat
      661 cggttcgtca tcgccaccaa cgagaacgac gagtttccca agttcatcga gaccggcgac
      721 taccggccga tccggccgtc ggtcaagtgc atctccaacg ccatgaacgt cggccacccc
      781 tccaacctcg cccggatctt ctggctctac ggcggagaga tggacgagac cggccgggtc
      841 gtccgggccc ccgacctcga ggcgatgagg cgggagatct tcgccgtctc cgtcaccgac
      901 ggcgagacgc gggagacgat ctccgaggcc tacaacaggt acgacgtcct cctggagccc
      961 cacggcgccg tcggctgggc ggggctcgcc cgctacctga aggaggtcga ggactggagg
     1021 ccttccgtct ccctggagac cgccgacccc gccaagttcc ccgaggaggt ggtccgggcc
     1081 gtcggcctgg agccgccggt ccccgccggc ttgtcccgcc tcgacgatct ggaggagcgg
     1141 ttcgacctcg tcgccgggga gtacggctcc ttcaaggaga aactctcgca gctcttccga
     1201 ggataggggg gtgagaggat ggatgacggg cgaaagatca tctttgacga cataggaagc
     1261 taccccctcc cgccggggat gagccgagag agcctcccct cgggcgactc ctacctggaa
     1321 ctggtgaggg acgccatggc ccagaaggtc gaggccggag tccagcgccc cacctacccc
     1381 cagtttcggg acatgatcag gatgttcatg gaccccatcg aggaccccaa gaggtcggag
     1441 agcccctacc tcgtccgggc ggaggaggcc cggatcctgg agctatcggc cctggaggcc
     1501 ctgggtcgag ggatggagga ggaggggggg aggctatcgg tcagggtctg cgtcaccggc
     1561 cccgtggagc tctacatatc ccggttcggt gccaccgact actgggacat cctcgataac
     1621 ctcgccagaa gcgtcggaag gttcgtcgag atggcgaaac tgagccgcca cttccaggtc
     1681 gccgtcatct ccatcgacga gccgagcctc gggatcagtc ccaacgtcat cttcgacgat
     1741 gacgagataa aaaaagccct ggagatcgca gcccgcccct gcggggggat cgactgccag
     1801 gtccacctcc actcccccct gatggcggag gcgtgctcca gcgtccccgg gatcaacgtc
     1861 atcggggtcg agtcggcagc aaatcccgac tacctcagcc tcgtcgaccg gcgggtcctg
     1921 gaggatcacg gagccttcct ccgggtcggg atcgcgagga ccgacatcga cggcatggtg
     1981 gcaaggctga acgaccggct cgagacgaac ctctggctcg acccccagag gctggcaagg
     2041 gaggtctccg cctccgaggc cgcggatctg atggagaaga ggctcgccga ggcgtgccgc
     2101 ctcttcggag accgggtgag gtacgcgggg cccgactgcg gcctcggctc ctggccgagc
     2161 cagaggatgg cgaaagattg cctcgcccgc tgcgcgaggg ccatcgcctc cttcgcctcc
     2221 aggggatagc tggaggggat cggcaatagg tggccggccg aggggccctg gccggccagg
     2281 gcttagaggc cggatcgacc ggcatgcccg gccagatccc cttcctaagg ggagaactgc
     2341 caggataatt tctcctcata cccggtatac ggggttatta tctaaaatcc gtccttcctc
     2401 ccgaagagga ggggattttc ggggaaatca taaccactat gtataaatat tttaagatca
     2461 ttctatgcgt catgagactg agggtcgtaa gttctaaaga ggagatcccg gagctgaacc
     2521 ccaacgagca gatgatccat ctcgccttca ggccgtctaa tatggacgtg atgaccctga
     2581 tccagaggtg cccccggatc cgaaccgtcc agacccctcc ctcttactac aggacgatgt
     2641 ccaaggcggc gaggcagttt ctggagatcc agggggtgga gctgctgcgg ggcgacgtct
     2701 ggggccacag aaaggacatc gacgagtact acgagatcga cgagggggtc attgagagga
     2761 tatccaaact gatctcggag ggtcggaaga tcgatgaggt cgtaactctg gtaaaaagag
     2821 agactaagct ctcggaagat atgatcaagt acatcctcaa gacgagcgtc atcctctgag
     2881 gggacctcgg ggaacctcca tcggcgggcg cccccccgcg tccgtcgggc ccctcaggcc
     2941 ccggagtcca caccatcacg attttatctc ataagagttg actcaaaggc cgaggattga
     3001 gatgaagccc tagatcggcg atctgatttc gggagggctt gcccggggga cgtcaggatc
     3061 ggaaggcggt ctccgggatg gtggatgagc atcattcccg gccgtcgaag aacctagcgc
     3121 cgatcgaggg cgatcaagcg gcccgatcct cgaagctggc cgttgggcat cgacgattgc
     3181 ggccttcggt gatgatcatg acaaaggttg tgcttaactt caagacctac tcggaggcta
     3241 cgggggctga ggccctgaag ctctcccgga tttgtgagga ggcaaaagat cagtacgacg
     3301 tcgagatcgc cgtggcggtt caggccccgg atatccggct gatatcggag aacgtatcga
     3361 tacccgtcta cgcccagcac gtcgacgggg tcgggttcgg cggcttcacc ggctccatca
     3421 ccgccgccgc cgtcaaggcg gcgggagcca ccgggaccct cataaaccat tcggagagga
     3481 ggctccggct cgccgagatc gaggcctccc tttcggcctg ccggaccgcc gggatctccg
     3541 ccatagtctg caccaacaac atccccacca cccgggccgc cgcctccctc aagcccgatt
     3601 acgtggcggt ggagcccccc gagctgatcg gtagcggcat ccccgtctcc aaggccgacc
     3661 ccggcattgt aacgggttcg gtagaggcgg tgaagaggat cgagccctcc gtcggggttc
     3721 tctgtggagc cgggatcagc cggggcgagg acctccgggc ggccctcgat ctagggtcgg
     3781 agggggtcct cctcgcctcg gggatcgtca aggcgaagga tcagaaggcg gccctggagg
     3841 accttctctc cgggatccgg aggtgacccc atgatctgcg tcagggtccg ggtctccggg
     3901 agggtccagg gcgtaggcta caggagcttc acccggagca acgccgcgga tctgggggtg
     3961 aagggctggg tccagaacct tcctgggggc ggcgtcgagg ccctcctcca gggggagagg
     4021 aagaaggtgg gggagctgtt gagcctgatg aaggaggggc ccgccggggc cctcgtctcc
     4081 gggatggagg tatccgaggt gccttgccgg gagattgagg agttcaaggt ggtccgctga
     4141 ggagggatgg tccaatgctt tcggtcaaca gatacaaatg cggctactgc ggggcctgcg
     4201 ttggggtatg tcccgtcgcc gccctggagc tggtggagac ctggatcgag gtcggcgacg
     4261 gctgtacggg ctgcggccgg tgcgccaaga tctgccccct gggcgccctg gagctggtga
     4321 aggaggcgaa gacgtgaact gcgacctggt ggtggtgggg gcggggcccg ccggctcgat
     4381 gaccgccaag gccgccgccg aggcggggtt ggaggtagtc ctgctggaga agaggcagga
     4441 gatcggcgac cccgtccggt gcgccgaggg cgtcagcaag gccgccctca agaagttggt
     4501 ggagcccgaa ccctcctgga tctctgccga ggtgaagggg gcgaggatcc gggggcccga
     4561 cggcacggag atcgtcatat ccgaggatcg ggcaggggcg gaggtgggct acgtcctgga
     4621 gaggaaggtc ttcgaccggg ccctggcgca agaagcggcg gaggccggag cgaaggtgat
     4681 ggtcaagacg agagcgctgg atctcctctg ggagggaggc gtccccgctg gggtcacggc
     4741 gcagttcatc ggcgagagtc tcaggatcga ggctcccctc gtcgtcggag ccgacggggt
     4801 cgagtcgaag gtgggccgct gggcggggat cgataccgcc ctcaaaccca aggacatcga
     4861 gacctgcgcc cagttcctcg tccaggacga taaaatcgac gacgactact gcgtgtttta
     4921 cctcggaaac gagatcgccc ccggcgggta cgtctggtcc ttccccaagg gcccctctct
     4981 cgccaacgtc gggatcggcg tcctcggctc aaaggccgac tctgggaccc caataaggct
     5041 tctgagggag tttatgaaga agaacttccc cgacgggaag gttctggaga tgatggcggg
     5101 cggggttccg gtctccggcc ccatcgaggc gacggtcgcc gacggggtga tcctcgtcgg
     5161 ggacgcggcc cggcagtccg accccatcac cggcggcggg atcatcaacg gaatgagggc
     5221 cggggtgatg gcgggggaga tcgcggcgga gctggtcccg aagggggagg tggacaagaa
     5281 atccctccag gtctacgaga agaggtggag ggagacgatc ggaaagtcga tcaccaggaa
     5341 cttcgccgtc aaggagttct tcgtcgaact caccgacgac gacctcaacc ggctgatcca
     5401 ctccctcgac ggcgtcgacg tcgcagaatt ggacctgagg gggctattga aggtcctggt
     5461 ccggcagaac ccgaagcacc tcctgaagct ccgccacctc atcgtctagt cgctgtctcc
     5521 ccgctcctcc gacggctccg ccctccggcg gctcagccgc cggataggcc ggggaggcgg
     5581 tcgaggtcga actcgatccg aacgctctcc cggtcggcgc cgatggagac gacggtggtg
     5641 tggaagacgt atcggagggt ccgccgcccc cccggcaccc tcaccccgtg gtcgcgagcg
     5701 agcctcgata gggtgcaggc ctggaggacg gggccggact ggaggaagag gaggcccgga
     5761 aggttgaggc tctggtggga tagggtcgga aacttctctt ccaggatcgc cgccagctcg
     5821 gtccgggtga tctcgaccgc cctcccctcg ctctcgaccc ggaggtgatc ctcgtcggcc
     5881 tcgacggcgt cgacgtgggc cttgagggtc gaggagatgt agttggcccc tctccccctc
     5941 ttctcgggcg ggaggagctc ttcgagcctg gggaggaagg ggcggaggag ggggccgctc
     6001 ccgccgaata gggcgcccgg caggtttttc acctccgccg agaggtctct tacgtccttc
     6061 ttcgtaactt cgacatggac catgcttctt tttcgggggg tcaaagggca tgtagttgtc
     6121 gggctgttaa cttacttcgt actcctaacc gatgctttta tcatttcttc tataaatctc
     6181 acagttagag ggaaaaagcg aattgggagc atcgcaggag ttcacagagc tagttagaaa
     6241 attcgataac aaagtcaaat ctgataagtc cggccattac aacgatacct ggctcctaca
     6301 agagtctatc aacaccctct ttagaacccc gaaatgttaa ctggacaacg aaatgagata
     6361 tgttaaggtt tagtacggga gcattcatat aggcacttca ggcccatgga tagagtaaaa
     6421 tggagctagt atgccacaac tggaacatat cgaagccatc gaaaaacgcc tctggaacgc
     6481 ggcagacacg ctccgcgcca actctaatta tgctagcaat gagtatttct tgcccgttat
     6541 gggtctcgta tttctgcggc acgcctacag ccgctatctg gcagtaaggg acaaaattga
     6601 ggcaaacttg cccacgcgtg gtggcaagac gcggccgctg atcaaagagg acttctcgca
     6661 acagaacgcc atcttccttc agccaaaggc tcaattcgac tatctcgtgt ctctccctga
     6721 taacgcggac cgagcaaagg cgatcattga ggcgatggag tcgattgaag cagattacga
     6781 gaacctgagc ggcgctttgc ccaagaccga gtatcaggag ctggataacg ccgtcctggg
     6841 acagctcctg cgcacgctca accctgatga gctggagaag gtctctggcg acatcttcgg
     6901 tcgcatctac gagtacttcc tcccccagtt cgccgatcag aaggctcacg acggcggcga
     6961 gttcttcacc cc
//
LOCUS       DASM01000164            3555 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_279035, whole genome shotgun sequence.
ACCESSION   DASM01000164 DASM01000000
VERSION     DASM01000164.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_279035"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          647 a         1108 c         1187 g          613 t
ORIGIN      
        1 cccttcttgg cgacgtcctc gatctcaaca tcgtactcct ttccggcctc cacgggggcg
       61 gagggtgcga agctctcgcc tcggaaagaa cctctatcat tgccgtacaa acacttcacc
      121 gtccttagcg aaaaaccgta gaacagtttg ccgcattgtc cacagatcac taccactatt
      181 aaatccttgt gctcccgagg agggggccgg accatgtcag gagatgcctg ggacggatct
      241 ctgggacggg ggtgaggggg tgcctccggg ggagcgaggc ccgatcctcc gactgggagg
      301 gggcgaggac ggggtccaac ccttccagac ggcggggaat atatcctata aacgcgaact
      361 gcggcggcat gaacggtctg gagaggtaca gggaggtgat ccccgacttc gacctcttcg
      421 tcgaagcgat aagaaggccg cttcctgtca ctgcgaggat caacaccctc aaggctgatc
      481 gggataggct gacatcccgt ctgcggaggg agaaggtggc cttcaccccc ttctcctggt
      541 atcccttcgg cctccggctg gaggtggaga agcccggaaa cctcctcgaa agcctgatgg
      601 gccagatcca catccaggag gagatctcga tggcatcgcc cgtcgtcctc agccctgagc
      661 cgggggagag ggtcctggac ctctgcgctg ctcccgggag caagacgacc cagatcgcca
      721 tgatgatgga gaaccggggt ctactggtgg cgaacgaccc cgccaacacc cgggtcgcct
      781 ccctcaggtc gaactgcgag agggcggggg cggtgaacgt ggcggtgacc agatacgatg
      841 gccgccgctt tcccgaccgg gggttcgacc gggtcctcgt cgacgccccc tgcaccggcc
      901 aggggatggc gaggaaggac attaccgtcc tgggccggtg gagcctgaag cggtccctgg
      961 ggattcagcg gctccagcgg gccctcctca ggagggcgtt acaggtgaca aggtccgggg
     1021 ggacggtcgt ctactccacc tgcaccttcg cccccgagga gaacgagggg gtcgtctcct
     1081 ggaccctgga gcaggtcgcc ggggtcggcc tcgaggaggc ctccgtccag gggctggcgg
     1141 ggagccccgg ccttgcggag tgggccggat cccggttcgg agacgagatg gagcggtgct
     1201 ccaggtacta cccccaccag aacgacgcgg gagggttctt tgtcgcaaag ctcattaaag
     1261 aataggtcgc ccctccagcc gatggatcgg gcaaccgtgg cggagattat gggatcgagg
     1321 ttcgggatcc ctcccgaagc cttcgagggg gtgagcttct tcctccgggg caggaacgta
     1381 tgggccttca gcctccccga gatccccgac ctggcctggg agacggtggg gctccggttc
     1441 atctcggtcc ggggccgggt ccccaagccc accaccgccg ccctccagct ctttggccgt
     1501 catgccacca aaaacgtcgt cgacctcggg acggaggagg ccctcaggtt cgcggcgggg
     1561 gagcggcaga ggatcccggc acccgtcgag gaggggtacg tggtggtatc tcatggggag
     1621 gacgtcctgg ggtgcggcca gtacctcggt ggagagctta tctccctcct cccaaaggag
     1681 cggaggatcg ggagggggga gccggaggat gggcggtcag gggggagcga cctctgatct
     1741 catccctggt ccggaagaca ggttcggagg gcactggtcc ctctcgggga ggcgggatcg
     1801 accccgccgc ccttctatct ctataccgcg agccattgag gctctttagg ctcgcaaacc
     1861 tcctccggga ggagatctgc ggcgacgagg tctcctacgt cgtcaacagg aacataaact
     1921 tcaccaaccg gtgcgtcgga agctgcagct tctgcgcctt caggagggcc gacggctacc
     1981 tcctcaccga ggaggagatc ctggggaggg tggaggcggc ggagaggtct tctgccaccg
     2041 agatctgcct ccaggggggg ctcgccccgg ggcttgtcct ggaggactac tgccggatct
     2101 tggaggcgat taaggaggat tatccgctga tgcacctcca cgcctactcc cccatggagg
     2161 tcctttacat gtcggagagc tccgggacta ccccggcgga gtcgatccgg gagctacagc
     2221 gggcagggct cggatcgatg ccgggaaccg ccgccgagat cctggtggac cgggttaggg
     2281 gggagatctg cccctccaag ctctccacat cccagtggcg ggagatcgtc accacggccc
     2341 accggctggg ggtccccacc acctccacca tcctctacgg ccacgtcgag aggctggagg
     2401 atcggatagc ccacctcctc ctcctccggg agatccaggc ggagacgggc ggcttcaccg
     2461 agttcgtcct cctccccttc atgccgaaga acaaccccct gggtgcccgg gcccggagga
     2521 tggagatcct agagaacctg aagatgcacg ccctctcccg ggtcgccctc caccccctca
     2581 tccccaacat ccaggcgagc tgggtgaagc tggggaggga cctcgccgcc aggaccctcc
     2641 tctgggggac aaacgacctc ggcggaaccc tcatggagga gaacatctcc cggaccgccg
     2701 gggccaccga ggcggagggg atcacccccg gggagctctc cgacctgatc gagaggtatg
     2761 ggcgggttcc tgtccagagg acgaccctct acggccgggt agggtgatct cggggatggg
     2821 gcccaccggt cccccatctt cattcggccc tctgcctggc ggtcccttcg tcgggcagct
     2881 ttttccttcg accccccggt ccgggggagg ctcagaggtg gcaatgacga caagtatgat
     2941 atactacgag caattctatt ttggggagtt tatccgatga ccagatcggc atctttgatc
     3001 ctgatatctt ccgtgatctg ccttctgtcc acagcctggg cctccgagac ggtcaccgtc
     3061 accggttcca cgacggtgat gcccctggtg gaggtctccg ccgagaggtt caacctgatc
     3121 caggatcggg tcatggtgac cgtcagcggt gtcgggggat cgggggtggg gataaagaac
     3181 gtcgctagcg gcttctccga catcgggatg tcctcccgga tggtgaggga ggacgaggtc
     3241 gagctctacg gcgacaactt ctccgtctac ctgataggct acgacgccat ctgcatcgcc
     3301 gtcagcgagc cgatatacga ggctggcgtc accagcatca gccgccagca gctccgggct
     3361 atatacgaag ggaacctgac gaactggcgg gatctggggg gtcccgatgc cgacatctac
     3421 gtcgtggcga ggatggttgg gtcggggacc ggcgacctct tcaacgagat ggtgatgggg
     3481 gacctagaga cagagaccct cggcgctgac acctacgccc agaacaacgg cgagatgaag
     3541 accgccataa ccaag
//
LOCUS       DASM01000165            3067 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_258536, whole genome shotgun sequence.
ACCESSION   DASM01000165 DASM01000000
VERSION     DASM01000165.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_258536"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          541 a         1005 c          836 g          685 t
ORIGIN      
        1 ggggactaca tgatcaccgc caggggatat aaggccctcc aggggatctc cgagctgtat
       61 tcggatctct tcgacctggg ggctggggag gagagagagg tcagccctcc accggcacga
      121 atctagcacc gcctgcatct cgcccccgga tagctggcct ggcagatgat ctccccgatc
      181 accgagcccg accatctcca agatctcctc tatcctttct ttctcctctt ctttccggct
      241 gaagaggagg ggcagctcga tgttctccct gctctgatgc catcctcgga atctcacgac
      301 cttatcatgg tcaggatcat cttgaagggc atgacggcgg cgaccgagtg gggctcgcct
      361 gctggcatga tcaccatctc cccgccggag agcctcatcg cctccccgga gatggtgatc
      421 tccgcctccc cctcgatgac gttcaccacg gcgtcgaagg gggcggtatg ctcgctcagc
      481 cgctgccccc cgtcgaaggc gaagagggtc actgtccccg tcctctcatc gatgagggtc
      541 ctgctgacca cagccccctt ctggtagccc acgagatcag ccagcctgag gggccggcct
      601 ttgagcccat cgggggagga cgattcctta ttcggttcca ttttctatct ctccacctct
      661 tttctatccc tcctggaact ggactcggat ggagaattct tcactcccgg atcttcctcg
      721 cctccagctt ctcgccgtgc ttcagcctca gcatgatctg cttagccagg tctgaggact
      781 tcaccatccg gatgtcgccc ctccggccga cggtggcggt gaagaccggc tcctttccga
      841 ggaagatctc cacccgctcg ccgggctttc cgccgaccca gatcgccagg tggcggccgg
      901 tgtcctcgat gtggggcgcc aggggctcct cccggccgtc gaaggtctgg acgtcgatgt
      961 ggatccccag ggtccgctcg atctcctcga tcctcttgcc ggacttgcct atgatcctcg
     1021 ccgcctcgtt ctcctggacc ttcaccaccg ccgaggagtc gctgatcatc tccacctcga
     1081 aggggccctt gaggtgggtt gccatctcct cctggacctg gagggtggcg aggtcccagg
     1141 aggcgggctt cttcccctcg ggggagatcg gcatgatgac gatctcctcg ccgtaggtgt
     1201 agagctcgta ctccgccctc ccggtctcga agtccttgac gacgatgacg ggcctggcga
     1261 ggtccgcctc cgtcatcccc gaggggacct tcaccatgaa ctcgacgtcc atgaccttcg
     1321 tcacctcccc cttctcgatg aagacgatgg tgtcgatcac ctgggggatc atccccaggt
     1381 cgaccctgcc gatcagcctc tggagggcgt cgatgggctt gttggcgtgg acgaccccga
     1441 tcatcccgac ccccgccatc ctcaggtcgg cgaagacctg gaagtccttc gtcttcctca
     1501 cctcgtcgta gatggtgtag tcgggcctaa ccaggaggag gatgtcggcg gaggcctcca
     1561 tcgacccctc cagggggccg tactgggtga tctccggcgg gacctggagg tctcttggag
     1621 cctccagggt cttgacgatg tagtcgcact tctggaggta cttggcgacc ccggcggcga
     1681 aggtggactt ccccgagccg ggggggcctg cgatcatgat cccccgatgg gcctccttca
     1741 gcctcgcctt cagctcgtcg ctgtggcggt agtcctccag gtcgaccctg gccacgggcc
     1801 tcactgccgt tatctccagg ccgtcggaga agggtggccg ggcgatggcg atcctcatgg
     1861 ggccgagctg ggcgacggcg gcgccgatct tctccatctc gatgtgggcg cccgggtcag
     1921 acttgaccct ctcgaagatc tccctcacca tatcccggag gtccctctcg ctgaggttcc
     1981 tatttccgat ctttacgagg ctgaactcgc cgaccttccc cctcttggcc atggggatcg
     2041 tcccctcctt caggtggatg gagagggtat ccggggtgaa gtacttctcc agggagatct
     2101 gcttgatctc ctcccgctgg ggtcggacgt actccacctc cagccccatc gcccccgcca
     2161 ccctcgactg gatggagtcg ctggtgagga agtaggcccc tatctccagg gccacgtccc
     2221 tgatcatggc gtcgatctcg ccgcctccgg cgagcttgat ctggtccagg tccggcctca
     2281 cccccacgta ctccagctcg atcttcccgg ccttcgccag ctccgagagg cgtctcagct
     2341 cttcaagccc cttgatcccc gtctcgcggc cgtggtttgc ctgggcctcc agctccgcca
     2401 ccaccgcctc ggggactaca actcttttgc cctgaaactc gccgctgacg accctggccg
     2461 aaaccatgcc gtcgatcact acgctcgtat cgggcatata gagaatcagg gggggatgag
     2521 cccctagcat acagtgtaac tgcgtcacct atattcagta aaaaaggcaa actaattggc
     2581 attagcaaat tttttgtaga tataacggaa cgcaagcgca ttgagcagac cttatcgcat
     2641 atggcaaccc atgatcctct taccggttta cctaatcgta ctctttttca tgaccgggtc
     2701 tctcaagcta tcgcaaggac caaacgtaca cagaaaacct tggccgtatt ctttatcgat
     2761 cttgaccgct tcaaagaaat aaatgacaca ttcggtcatt ctaatggtga ttacctgctc
     2821 ctgactattt ccgaacgcct ccagtccatc ttccgagata ccgatactgt agccagatta
     2881 gggggggatg aatttctcat tctggtaaat gaaattataa attctgaaat tttattcgag
     2941 cttgccccga aaatcattga aaccatccgg caacccatcc ccatttcggg ggaactgctt
     3001 cagatcggag ccagcgttgg atgggctatt tatcccgaag acggagaaga cgagctgaca
     3061 ttgataa
//
LOCUS       DASM01000166            6655 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_188905, whole genome shotgun sequence.
ACCESSION   DASM01000166 DASM01000000
VERSION     DASM01000166.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_188905"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    4484..4515
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1078 a         2186 c         2209 g         1150 t
ORIGIN      
        1 tggcatatgc aaaggtcttt gggaaggcca catactgggc acatctcgga actcatcttc
       61 tggtagttac ctccacagaa tatatgaact cattccttgc atcccctatc ggtcctgcag
      121 ttgagccaat cttctgattg acatgaaatc gatagggtaa atagctattg gtggccgggc
      181 agaccggaaa aagacccccg gatggatcat caagaacctt tcgccccgcc ggtggagggg
      241 accaccggga tctccgttcc cgaagggtct gcccctcacc tcctcacctc cccaagcctc
      301 ctcgcgatca cctcgccgag gaatatggcc agggcgagga atagcagggg caggcggcgg
      361 ctgaccctct cctggacggt gatctcgctc ctcctcctcg cctcctcgac gaggctcctc
      421 gccacctcct cctcggtgaa gaccatcccg ccggtggaca tgatcagcct cgggaagtcg
      481 gggttatagc cgacgaacct gtactccagg gggtagttta cggcgatccc gtagtccccc
      541 acggtgtata tcccctcctc ctcgggggtg atgaccgcag agtaccggtt ctcccccacc
      601 ctctccgcct ccaccccctc gatcctgggt gggctcgggc ttgtgatcgt cagctggagg
      661 ggggtccctg cccaaccgtc ctcggcgtcg accctccagg cctcgggcct ggggtcgccg
      721 atcgcccagt tcaccatcct ggagacgagc ctcgagctgg gggcggagta gacctccggc
      781 gcccaggcgg tcccgtcgtc ggtggtgagg gccgccaccc tccccaggcc gtacctccag
      841 gtggtgagga tcggctttcc gtcgagcatc accaccagcc tctggccgcc aggtttcggg
      901 gtgacgtcgt tgaagccggt ggcgttggcg gcgagggcca ggtcggaggt gatgtagtgg
      961 ttctggtttg cgatcatcag ggcgtagccg ctggcaggga cctcctccac ctccggcacc
     1021 tcctccacct ccggcctctc ccccgcccgg atggtcaccg accccggata aagggcgggg
     1081 tggtactcag ccccgctctc ggcggcaagg tctctgaacc cgccggctgt cccctcaaag
     1141 gccttcacct ccaccaggtg gaccgtcgcc cccatctccc cgatcatcct cgccgactcc
     1201 gagaagacct cggggtactc ccggatcctg ccgtcggaga tgacgatcac ctccccctcg
     1261 ctgctgctgg cgttgaggag atcccaggcg agggcgaggc cgacgttcag ctgggtctcc
     1321 tcctgggcgc cgggctgggg ggagaggctg gatatccggt cctggatcat catctccttc
     1381 ccggcgatgg gggtgggggt gaagacgacg aaggcctcgg tcccgaagac gacgagggcg
     1441 acccggtcgt ccctgaactc cggcgccttg aggagctcga tggccagggc cttctcgtag
     1501 tcgaggaagg tggctccgcc caccgacatc tccctccggg tgctccccga gatgtccatg
     1561 atgaggaccg tcgtcttccc cccctcgaag atgctgggga ccgccttcac cgggaggatc
     1621 tcctcgaggc tcgagttcct gtattcgccg aagtcgaagg agttcttccc ccccaccacc
     1681 acgagccctc ccccctcccg gacgtaatcc ttcagcccgg agagctccgg tccgtacctc
     1741 tggtcgtcga ggactacggc cttgaacctc ttctccccgg cgtccagctc ctcggccctg
     1801 aaggtggggg agacggtcag cctgtagtgg tcagagagga ccctcgatag gggagcctcc
     1861 ccctccgcca ccaggaggac ctcgggcttg gggacgacgt agacggcctt ctggtagcgg
     1921 tcgttctccg ggatggcgtc cgtcgggaag ctgatcgtcg cctccagggt gtgggttccc
     1981 gtcgttggaa accggtggga gatcttgatg gaggcggacc tcccgtctcc cgagatccgg
     2041 ttctggtaga tgaccgcgcc gtcggccctg acggtgaggt ggccgtcgaa ctcgcccccc
     2101 gagctcctga cgacgacggt gaaggggtac tcccccccga ggacggcggt gttcgtcccg
     2161 gagatctcga cgctccggtc ctctctgagg ggggccatct ccagggcgaa gacggtggtg
     2221 ttggcggacc gggcgagggt gagggcctcc tccaggtccc gcccccggtt gctgtagccg
     2281 tcggtgacca ggaggagggc tccgccctgc tgggcgtact ggaggacctt gtcgcccagg
     2341 ggcgtcgccg tccccgagaa gctcctgatg gtggagtcgg ggaggccggc caggaccctc
     2401 tccgccgccc cccggtcgaa gatctccatc gaggcggtga ggtccgagac gacggtgatg
     2461 atcggccgcc tcgcggaggt agtctccgtc accgtgacgt aggggtttgc cagggccacg
     2521 accaggaggc agaatatcgc cgtcctgctg aggacgagga gcctgtttct cccctttcgg
     2581 aggctcagaa gccccaagat caatatcaga ggtatgagcc ccaggaggcg gggctccgag
     2641 aagtagagcc cctccatcat cttcctatcc tccggaggat ggcgagctcc aggattatga
     2701 gggcggcggc gattaggacc atccaccccg agaggtcctt tcggaccagc ttctcggagg
     2761 tcctgccgga ggcgaaggac ccgaggttga gggtcctccc cccctggagg ttcgactcga
     2821 cgggatcgta catgttggcg gcgatggttc tcccctggaa cccataaact cccaaccggt
     2881 ccaggaggag gacgtctgtg gagatcctcc cgcccggggc ctccaccagg gccgtctccc
     2941 cgaggcgtat cacctccccg gtcttccggt tcgactcgga tatgtcgggg acctccgtca
     3001 gccagttcgt catcttgtac cagaagaggg ggtactccgg cctctggctg aagtcggagc
     3061 tctcctggga gaggccgagg tagacgacgg tccctccccc gagcctccag taggatatga
     3121 cggggactcc gttcgcctcg gctatcgtca ccgacccccg ccggggagag gccagaaggt
     3181 agcccttgac caccacctcg tccaggtgga ggtccccggt gaaggccggg ttcctcctcc
     3241 agaggggggc ggactcgttg acgaccccgc cgatcctgac ggggaggtac tcggggctgg
     3301 cggaggcgtt ggtgccgatg tagacgaccc tcccacctcc gtagacgtat cggttcagct
     3361 ggccgtcgtt tgtggagtca tccgtcacca ccaggtcgaa gcctccatac tcgccagagc
     3421 ggccgatctc tatccccgag atcgacctga gggcggcgag gggggcgccc tcctccccca
     3481 ggtagagggc cctcctcggc ctcacctccg ggacggagac gtaggccctg ttgtcggagg
     3541 agatggcgtc ctccacctcc agggtgacga tggtgatccc gggggaggcg tcgaaggtga
     3601 ggtagctctc tcccccagac ccgatcgtcc tggtggtggt ggtggtgcct ccggccccct
     3661 cgatcctgat ggggacggtc ctggtcctgc cgtagtttct tatgaggcag gagcagtttg
     3721 cgatcccccc ggagtcgatg atccagcctc cgacgatccc cacgttatcc cccccgcccc
     3781 cggtgtcgac gaagacgacc cccgtcccct ccgtctcgat cagccccctt gtcacctcgg
     3841 ggtcgtcccc gatccagctg atgaagtcgg agatgacgag gatctgcccc ccctccgccc
     3901 ccaggaggct tctccccatc accatccccg ccgagaggtc cgccgagacg gcccgggggg
     3961 cgagggcgct gaaggccgac ctcgcctgga cctcgtttcc ctcccggagg gcgaggaggg
     4021 ggatcgtctc cgcgaggacg atgctgatcc ggtcctggcc ggaggagtac cggaggaaga
     4081 tcgcctccgc ctcgtcgaag ctcgcctcca tgctggcgga gaggtcgatg accaccacca
     4141 ggtgctctcc ctggatcccc cgcccctcgg tgtaggggcc ggcggcggcg atgacgagga
     4201 agacgatcac cagaagctgg gtccagaaga gggggtcggt gaccagccgg ctgagggagg
     4261 cggtgggctt cgcctccccc tcgaggatga aggccgtcga cgggaagggg aggtccctgg
     4321 gcttcggcct gatcatgtag aggatgatca gggggacgac gctcaagagt cccaggaggg
     4381 ccagggggct gctgaagggc atcgccaatc ctacgcccac ggccgagaaa aggtttgcgg
     4441 aagcttcggc gcttcgtccg tggcaacatt cttaatacga ttgnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnncgagg actgcacccc cctggtggag gagccgaagt acgagaagaa
     4561 gctggcaggt ctgagggagc ttatggcccc cctcctcagc accgaggcca ccgagacggg
     4621 catggtgatc catccggaga ggtgttacgg ttgcggaaac tgcgtcgtcg cctgtcccgt
     4681 caacgtcgcg agcgacccca aggggacggc cataggccgg ggccccgtca gcggcaaggc
     4741 gatcctgatg gtggaggacg gcgtcgtcaa gtgcgtcaac gtcgatcagt gcaggaggtt
     4801 tgggcccgag aaggtcctat gcaacgcctg catcgtcacc tgtcccagca aggcgatcga
     4861 gttcatctag gtgatcaaaa tggcgttctg tacaggttgc tctctattgt gtgacgacat
     4921 cgaggcggat tttgatggga tgcaggtgaa aaggaccctg aacctctgca ggaaggggcg
     4981 ggcccactac gactcccttg ccgtcgacag gctcaccccc accgccggcg gctctgaggt
     5041 gagggtcgag gaggcggtct ccgccgccgg ggagctcctc gccggggcgg accgccccct
     5101 cctcctcggc ttcggccggt cgaccctgga ggcccagagg ctggggatcg agctcgcgaa
     5161 gacgctcggc gctgccatcg gcgaccccac ctcccggcag gggctgataa cggaggggat
     5221 tctgaagggg gagcttccca cctgcacctt cgacgacgtc cggaattacg ccgacgtctc
     5281 gatatactgg ggggacgacc cctccagcag ccatccccgc cacctctccc gcttctccta
     5341 cttcccccgg ggggagaaga ggcagagggg gtacgaggag gaccgggagg cgatcgtcat
     5401 agacgtccgg aggtcgccga cggccaccat cgccgccgac ggcttcttcc aggtcccgcc
     5461 cgggggggac gccgccttcg ccgaggccct gacggccgcc ctggggggga agatccccaa
     5521 ggtggaggat aaaaagaaga tgctgaacct ggcgacgaag ctccggaagg cgaagttcgg
     5581 ggcgatcttc accgggccgg ggctcgccga tgcccagggg gacctcggct ggttcttctc
     5641 cctggtggcg aggctgaagg agctcacccg gttctccgtc atccccagcc tccccgagta
     5701 caacgcccgg gggctccacc aggccctctt cgacgccgcc gggtcctcag gccccgtcac
     5761 cttcggggac gagggaggct cctgcctggg gttgccgggg cttctggagg ggtgcgacgc
     5821 cgtcctggtc gtcggctccg accctctggc cggccttccc gcccccctgg cgaggcggct
     5881 ggcgaaggtt ccgatggctg cggtcgggac ccatagaaac ttaaccaccg acgccgccgg
     5941 ggtgacgatc cccaccgcca tggcggggct ggaggtggga ggggccgccc tccgggccga
     6001 cggggtgagg gtcgacttcg attctgcggt ggaatcggat cgctccgccg atcttcagat
     6061 cctcgagatg ttgatggagg cggtctagat ggcagagatg gaagagcttt taatcgtcac
     6121 gtaccggggg atcttccagg aggtggagga ggccctgggg atctacagcg actcgtaccg
     6181 cgaggagagc gccctcctat tcctggaccg cggcgacgcg ttggggagaa agatccaggc
     6241 cgacagcccc gtcctcctgg agacggccac cggtaaggtg gtggtggtgg cgaagatctc
     6301 cgaggagccc catcccggga tcggcttcat gccgaggagc ccctggtcgg ctcagctcct
     6361 ccccggcgag gcgggcgagg gcgtcctcct ccagatgaag aggttccagg cccgggtcgg
     6421 ccctgctggg gaggcggtga ctacgatcga ggagatcggc gagaggatcc ggggtccctg
     6481 accctccgga ggcggctaga tgaaccttat atccatcaga gacctctctc cccgggaggt
     6541 ggaagcggtc ctggaccggg cggacgagct gaaggcggga aggtgcgacc cctccgcccg
     6601 atcgatcctg gcgggaaaga gcctcgcggc gatcttcgag aagccctcca ccagg
//
LOCUS       DASM01000167            9289 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_16315, whole genome shotgun sequence.
ACCESSION   DASM01000167 DASM01000000
VERSION     DASM01000167.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9289)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_16315"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1832 a         2790 c         2845 g         1822 t
ORIGIN      
        1 gatgctcaac accctctacg gcctccaggt tgatacgacg gatctggagg agagggcgga
       61 ggagatcgag ctgcagatgc agaagctcgc cgatcaggtg cagagcacct ctgcggaaga
      121 ggttccgggc aagatggagt tgcagttccc gatgtacggg tgatgaagat ggaatgcgtt
      181 gcacttactg gaatatcaag gccggtgatc agggagctat tgaccaacgg gatcaggacc
      241 ctggagatca ggagccccgg caatttcctg gccctcctcc acgccaaccc cggggacgtc
      301 atattcctga cggaggcgag cggacccgac atcgtagccg ggacgggggg gatgctgacc
      361 agggtccggg agatccaggt ggtgacccac aaggtcgtcc aggccggagc cgacttctac
      421 gaggagaggg agtctcaggc ggcccgggtt cagctggcga tggtcggcca cggcagggtg
      481 aggcgggtga agatcctcaa gatcgggtcc ccgacggtcc tggaagtcga cagcgtctgc
      541 tactacgatg caggttaggc tgggtgccac ccctccattg cccaagccaa aacggctctg
      601 ctgcaggccg gaaaaaatga tgggggtccg ctcgagggcg gaacccctac ctcaggccgg
      661 agcttcgacc aagtccttga tctcgtaggt gatgctgacc gtcgccgtca ccttgacgtc
      721 gccgggggct atcggcgtcg ccgcccccgc atcggcggcg aagaagacct tgctctccgc
      781 catcgggatc ggggccgatc cgccctcaga gatcgatagg atccggccga ggctcacccc
      841 ggcggcctcg gcgatcaccg ccgccttccc catgccgtcg aggacggcct cctccagggc
      901 ctcctcccgg accggggtcg ggtcccggag gccgaaggat acgccatcga tggtgttggc
      961 tccggcgccg acggcggcgt cgatgatccc gcccacgtcc acttcatcgg tgaggttgag
     1021 ggtgaaggtg acccggtttg agacctcgaa gatgaccttc tcggactgtc tcccatcgat
     1081 ccagtctcgg gtggggttga tcgagtagct ggaggtcttt atctcctcct cggggacccc
     1141 ggccgcctgg agggcctcga tggaggcggc catacgtcgg gcgttatcgt ccgccgcgtt
     1201 ggccgcgctc tcgtccctcg tctggactcc aagagttatc gtcaccgaat cggaatcggc
     1261 agtcgcttct cccgtccccg ataccgtcag ggtcgatacg tctccgcttt cataactctc
     1321 tcctagtgcg ttgccagaag acgcggaaag caccataatt cccagcagaa gggcttcgaa
     1381 gatcttcatg aataaacccc tataaatgat agatccgaaa gtaattaaat aaatcggtcg
     1441 gtcttcggcc gcgggtggaa ccagaggatc ccatcagcga tgagggggat cgtcaggtgg
     1501 accgcccttt gaaggatcat ccatctcctg agccctccag accccggcga gatggccgtt
     1561 agtctccata agctctctcc agctgccgga ttcgacaaat cgccccctct ccaggacctg
     1621 gatgattacc gaggagcggt tttgccctcc ggagatccaa ccaccatctt ttaatctcga
     1681 ctcctcgatc cccttcaccc atgaggaacg tcggaaagtc catcaggatg gagaggatct
     1741 tcaacagaag gacggggcgg acggtcatcg tccccatgga ccacgggctg acggtggggc
     1801 cgatcgaggg gctgatagac ctccaggagg cggtggacaa ggtggccgaa ggcggggcga
     1861 acgccgtcct cggccacatg ggcctccccc tcttcggcca ccgggggtac ggccgggacg
     1921 tgggtctgat cgtccacctg tccgccagct ccagcctggg gcccgacccc aaccacaagg
     1981 tcctcgtcac cgaggtggag gacgccatcc ggatcggcgc cgacgccgtc agcatccacg
     2041 tcaacgtcgg ggcggaggac gaggccacga tgctccagga cctgggacgg gtcgctcgga
     2101 cctgcgacgg ctgggggatg cccctcctcg ccatgatgta tccgcggggg gcgaaggtga
     2161 aatcggagca cgacgtcgag tacgtcaagc acgccgcccg gatcggtgcc gaactgggcg
     2221 tcgacatcgt caagaccaac tacaccggat ctcctgagac cttccgggag gtagtcaaag
     2281 gctgcagcgt accggtggtg atcgcgggcg gccctaagat ggatacggaa caccaggtcc
     2341 tggagatggt ctacgactcg ataaaggtcg gagggggagg cctctccata ggaaggaaca
     2401 tcttccaggc caaagacccg accctctttg tcaagaggct ctgcaagatc gtccacgaga
     2461 actactcggt ggaggaggcc gaagaggtca ccctctgatc gcccatactt ttccaatttt
     2521 gaaggctaga ggcggtggaa ggcgcgattt tataaataag gtcaggacca ctaacgacta
     2581 ggagatctgg ttctccaaga agtggttcct tgttcgagct ttacgtcgcc tcaaagcacc
     2641 tcgtttccag aaggagacag accctcctcg cggtgggagc tgtcgccatc gccgttgcga
     2701 tcaccatcct ctttcggtcc ctgataaacg gctttgagac gaccctaaac gggatcatct
     2761 tcgagttcgt ccctcacgtc accgtccttc ccgaagatgg cgaaaattac atccacctct
     2821 acaagaccct catcaatgcg atctgggcta ttcccggcgt tgtggccgtc tccccggccc
     2881 tctcgacgac ggcatctttc gcccacgacg ataacgtcga gaatgtcgtc ctccggggcg
     2941 tcatccccga cgagctgaac aagatatcga agatcgggat cgattacatg gtggagggcg
     3001 acctcgactc catcgagacc gggaggagag tcgccctgga ccaggggcta gcagacgagt
     3061 tggaggtgaa tctcggcgac tcggtctggg cgagctttcc cgacgcgagg acgatgagcc
     3121 tggtggtctc gggcatcttt gataccggcg gattttcaac gaactttgcc tacgtctccc
     3181 tagatacggc ccgggagttt cttggcgagg gaaacgtcgt aaccgacgtc cagatcaagc
     3241 ttgaggatat ataccgggcc gacgaggtag caagcgagct tgaggctcgc ggttacaatg
     3301 ctgagagctg gcagagcctc tcggggatcg tggagacgat cgagagccgt aggttctcca
     3361 acaccctgat catgctcctc gtgatagtga tcgcatcctt cgggatcgcc aacatcatga
     3421 atctcctggt cctggagaag acgagggaga tcgggatgat tatggcgatg ggagccaccc
     3481 ccaagagcgt ccgcaagatc ttcctcatcg agtctggcat tctggggctc gtcggtggga
     3541 tggcgggctg cctcctcgga tatctcatct ccgcctacgt caacagcctc gccatcgccc
     3601 tctcctccgg cccccgcttc tcggtagtgc tgaccttcat cgtcgacccc tgggacctcg
     3661 tcgccttccc cctgatgacc cttctattga gcgtcgtcgc cggggtctac cccgcccacc
     3721 aggcctcgaa gctcgatccc gtcatcgccc tccggagctg atccgagaag gcttgcgcaa
     3781 aagatgcggt tcatttcagc ggcctgggtc tcgatttgag ctgatcgggc ccgatctcta
     3841 taaataagat ccgaagaacg gttactcgca aaaaagacgc cttatgatcc ctgaggagtg
     3901 attccttgct cgatctgttt gtggccacaa agcacatcat gaccaggaag agacagaccg
     3961 ccctcgctgt gggggccgtc ggcctggcgg tggccatcat catagtcttt cggtccgtca
     4021 tgaacgggag ctttgataca tacttcaggc tcttcttcga gctggccccc cacgtcctgg
     4081 ttctccccaa ggaaggtgag gaatacatct acctatacaa gactctcatc gatttcgtct
     4141 ggacgatccc cggggtcgct gccgtctctc ccgccctcag cactacggcg accctctcct
     4201 tcgaggacaa agtcgagaac gtcgagatga ccggggtctt cccggcggag ctgaacaagg
     4261 tgaccagcat cggtgacaag tacatgatcg agggcgactt ctacgccata tccagcggga
     4321 gaagggtcat cctgggggag aaggtaaccg agaagctgga cgtgaagata ggagagaacg
     4381 ccgtcgccag ctttcccgac gctacgacca cgaacctggt ggtggcgggc gttttcagga
     4441 cgggggtcga ggagtgggac cagtcggctt tcgtctccct ggagacggca agagggtttc
     4501 tgggcgaggg ggacgtcatc acctacatca acatccagct tgacgacccc tatcaggccg
     4561 acttcctcgc cgacgagatc tccgcccggg gatacgacgc cgaaagctgg cggagcatgt
     4621 ttcccgagtt cgaggagacc ctggcctttg agaccttctc caacaacctg atcctggccc
     4681 tggtccttct gatcgcctcc ttcggaatcg ccaacgtcat gaacatgctg gttttggaga
     4741 agacgaggga gatcgggatg cttctggcca tggggacgag ccaatcccag atcaggagga
     4801 tattcctctt cgagagcggg attttggggc ttggcggtgg cgtagctgga accgtcttcg
     4861 gttacctcgt ctcggcctac ttcaacagcc tccagtacac cgtcaacgcc ccgacggccc
     4921 cccagcctat agtcatccag ttcctcgtca accccttcga cctagtcgcc ttccccctcc
     4981 tcgccctgat actgagcacg atcgcggggg tctaccccgc tcacaaggct tcaaagctgg
     5041 acccggtggt cgctctccga ggttgagata gatgttcgag ctttcgatcg cggaaaggca
     5101 catcctcgga aaccccagga tggttctctt cactgtcctc tcggtagccc tggcggtggg
     5161 ggttatcgtg gttctgatag ggctgaccga ggggtacagg gaaaacctca tcgagaacac
     5221 cgtggagaac tccgctcacg tcaccgtcga ccctaaggaa gacgaggact acatctatct
     5281 ctacagaacc ctctccgcca tcgcccggga ggaccccgag gtcctggcgg tctccccgag
     5341 gctggtggga agggcggcgg ccaagtacaa ggacaaggtg gagggggtca ccttcatcgg
     5401 ggcggatccc atacaggagg atatcctact gaggattcag gaggacgtgg tctggggcga
     5461 ctactacgac ctgcggttca agaggttcgc cgcagtggtg gggtcagacc tcgccgacga
     5521 tctggagctg aaacggggcg acgacttccg gctcacaagg caggacgtca ccatccaggt
     5581 gaagaccgtc gggatcatag ataccgggac gggcctcgac cagaccctcg tctacatacc
     5641 tctggagacc gcccaggacc ttctggggga gggggacgtg gtaactgagg tgggaataag
     5701 gctttctgag atcgacgcgg ccccggctgt agccgcggat ctcaacaaca aaacccggta
     5761 taaggccgaa agctggcagg agaagagcag ggacatcctg gagcagctgg acacccagca
     5821 gatatactcc gccatattct acctgttgat cttcataata tccggctttg gcatcgccaa
     5881 caccatgata atgataataa gccgcaggac taaggagata gggatcttga tggcaatggg
     5941 tgcggacggc atctcgatca tgaagatatt cgtcctggag agcgtcatcc tggggccgcc
     6001 ttcggccctc ctcggcggcg gcctcgccta cgtcgcggca aagttgatcg ggactataga
     6061 ggttccggct gagatctaca tagccgacag gatgacggtg attcttgacc tggggacgtt
     6121 cgtatacgtt gccgtctttg ccatcctcgt caacttcctg gcggggatat atcccgcctt
     6181 caaggcctcc aggctcgatc ctgtgaaggc aatagcaaaa gaatgagatt cggggaggcg
     6241 atctggcttt gaccaacatc atagagatca agaagctgag gaaggtctac ggggacgggg
     6301 tggaggtggt ggccctcgac gacgtcgacc tgacgataaa ggagggggag gtcctatcga
     6361 tcgtggggcc cagcgggagc gggaagtcga ccctcctgaa catgatcggt ctgttggata
     6421 cccccacctc cggggagatc tggctgaagg ggcagaacat aaccagggcg acccccaaag
     6481 agagggcgag gctgagaaat aaggagctgg ggttcatatt ccagtttcac cacctactcc
     6541 cggagttcaa tgccgtcgag aatgtgatga tgccccttct gatcgccggg gtcgataggg
     6601 acgaggcgag ggatcgggcg atagcccttc tccaggaggt ggggctcgcc gaccggctga
     6661 tgaacaggcc aaaccagctc tccgggggcc agaaccagag ggtggcggtg gcgagagctt
     6721 tagcgggcca cccctccatc gtcatcgggg acgagcccac cggaaacctg gacaccaaga
     6781 gctccgacat gatatacgag cttctgagga agctcaaccg tcagatgaac cagaccttca
     6841 tcctcgtcac ccatgacatg accatggccg agaagacgga taggatactt cggatcgtcg
     6901 acggcaggat cgtatgcgag atgcgccgag atggggatga gttcgtcacc caggaggtct
     6961 gtcgggcgga atgagatccc ggccccggct gcccatccat aggtttaaat agcctgccgg
     7021 catctccagc gagcgtgaga agctgttttc gcggtttttc gcaaaatgga tgtgaactga
     7081 gtgtacggaa acgatcgagg atccttcagc agtggcggat tcacaagctc ttctccagtt
     7141 gaggctggaa agacgtatga ggtggagatt gaggacatat cgaggcaggg agacggcatc
     7201 gcccgggttg agggcttcgt catcttcgtc tctgatacga aggtcggaga caaggtctcc
     7261 atatccattg acagggttat gcgaaggttc gccatagccc acaaggtctg atattatctg
     7321 tccttttgat catttagatg taaaatggtc ggcgcagcca tccgctgcgc cagggccctt
     7381 ttagacgatt gcgtctcccc ctctccttcg tttcttcctt cctccttggg tccatcgctt
     7441 atccggacct ccgggcagga gttcgtcagg gccgaggtgc cgatccggag ggcatatctt
     7501 caagaggcga gggatcgaaa gggagaccat gaaagggcgg gggggccatc ctgcggatcg
     7561 accttcatag aaataccgcc ttcatcctga cggcggccgc ggccctgggc cttctcttcc
     7621 cggctccggc ccgggggctt gagcccctcc tgatcccggc gctatttctg atgatggcct
     7681 tctccctcgc cggaatcgac ctcgaccttc cgccgatgcg gggcgcagtg gcaggatttc
     7741 tcgcaaacta cctcttccta tccggcctca tcctctccat cgctcccgcc gtcctggagg
     7801 aggaggccct cgtcctcggg tttgtggtga tggccgcggt ccccccggcg gtggccgtca
     7861 tccccctgac caggcttctc gccggcgacg tccgcctcgc cctctactcc gaggctctct
     7921 cgtacatcgc ctccatcgtc ctgatgccgt cgatcatcta catcttcgcc agggatgcgg
     7981 gagatggggc tggggtcggg ctcggcgaga cggcgaagat cgtcatcctc ctgatcctcc
     8041 ttcccatcct cgcctcaagg tacgtcggac gcctgggtat cgatcccgcc caccccatca
     8101 acggaggcct cttcgccgtc acctacatcg tggtgggtca gaacagcggc gccatctcgg
     8161 gggaggcggc ggggctcctc ttcatcgcca tcgttcggac cttcgtctcg ggggcggcgg
     8221 tctacgccgc cgcggccttc gtcggggtcg aggcccccca gaggatcgcc ctcaccctct
     8281 ttgcctcctt caagaacctg gggctcgccg ccgccgtcgc cctcctcatc ttcggccccc
     8341 gggcgggggt cccggccgcg gtctgcatcc tcaccgagac cgccttttat atcctcctct
     8401 cggccttagc agggcggcgg gacgggaagg tggttactga aacggggggt tgacccgaag
     8461 ggatccacca aagggcgagg ccgaatagat ctcgacggct gccgaccttc ttgcatagcg
     8521 gcagcagggg tgacagatcc gctgcccccc ctcggggcca catatccgga aaaaattgat
     8581 gatccgggga tcggtatccc ggtcagatgg gcatcaggac cttcggcgtc ccgacggtcg
     8641 gcatcgtcga aaatcgggca taggttccga ccgtcggccg gctgccggcg gcatccctcc
     8701 tctccttgaa cccgacgtcg gagagccact gcctggcgag gttggtgtag tagagggtgc
     8761 tcgtcatgct gctcaaatcg aggcggtata tgttctggcg ccagtcggcg tagtagaggc
     8821 ggccgtcccg gtagacgagg ccggctatgg agccctccct cgagtcaaag atcctggtca
     8881 cagccccggt gccggtggcg accttgtata tgctggcggg aaccctattt cccgagctga
     8941 ggtagaggtt tccctcgtaa tcgaaggcaa agtccccggc ccagaatcca ccgacgtcgg
     9001 cgaggcggac ggtgtagatc ggggtcgcag cggctccgtc gaccctatag atcttgccgt
     9061 ctcctccagc ccccgtcgcc tcgctgaagt agaggccgaa gttcccgttc tggtcccagg
     9121 cgaaggctac gtcccggacg taggtggtgt gggtgaagac gaccgactcc gcggcccagc
     9181 cagatccggt ctcagcggtc aagtaaatgt tgaactcgtt ggcgttggcg tagtagagct
     9241 tctcggggac ccctggatgg aaggtgaagc tgtagagcct ccccgaggg
//
LOCUS       DASM01000168            6577 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_77124, whole genome shotgun sequence.
ACCESSION   DASM01000168 DASM01000000
VERSION     DASM01000168.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_77124"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1129 a         2037 c         2164 g         1247 t
ORIGIN      
        1 agaaggagag ggcccagagg atggcggagg gctgagggcc cgccctcatc tcctccccca
       61 atcctctcat tcttccccct tccttccggt ttccgccctt ccctccccct gcttctcatc
      121 ccatccctca tctccagagc ctcatctcca gagcctcatc tccagaggtc tcggccccgg
      181 tgggagacgg cggggtcgac cacgatcagg tcctccgggt agggctcaaa gaaggtctgg
      241 ctccccaggt attcgtcctc gaagcgggcc acccaggcct tgaggatggt ggtgttgggg
      301 cagatagtaa ttttgccgtc ccggtacctc tggacgttat cgaggaagat cgccttctcc
      361 cccgccgaca ggcggccttt gaagtggccg aagatgtgca tcaggacgtt gatgttggag
      421 gtatacctcc gggccctgca tagcgctttt gccaggtggg gctcgtagtc gcccgccacc
      481 tcggggaaag gccgatggtc gtggttggcg gcgatcgccc ccagggccct cgcctcagcc
      541 tggtggtagg cgaggaggag catcttgttc tccgtctgga accggagtag ctcctccatc
      601 gaccccgacc tcttcacctc ccggaagctc gccagggtga agagcttcgt catgaagtgc
      661 tccttgatcc tagggttccg gagcctcccc tcgtcctcga cggagaggtg ggggtggcgg
      721 gccaggacgg cggcgccgaa gaggccgctg gcgctcctgg tgagggccgc cgacctcccg
      781 gcggcggggt agaccttggc gttcctgatc ccgcaggagg gggagctggc cttgaggatg
      841 aagccgtcga cgtcgccgac ggcgtcgagg aaagatccgg cgaaggccgt catctccgcc
      901 gtgacgtccc gacccgttgc gggctggatg agcctcgtct ctttctcttt gccggtagag
      961 acgagcctca ccgagtccct ggggacgccg aggccgatct ctacctcggg gcagaccggt
     1021 acgaactgaa cgtaaggctg aaggctcttt acgaagtcac tgctgatgat ctggccgttg
     1081 tacctgcagt ggtcaacctc gatgcaccgg ctcaccacca ccctcggcct gggaaactct
     1141 ctcaacgcaa accacccttc tcccatcatt ggggtcaaag ctacttaaaa ccgacgagaa
     1201 aaattgcccg agcggtttcg gcttctggaa ggggatcatc gctcatgatc gtctccccgt
     1261 ttgtgggggt cagattgatg atcatgaaag aacaattaaa accgcgatgg ccgatagaga
     1321 gcgaattctg atcctggggg ctgcaggccg ggacttccac aacttcaacc tgttcttcag
     1381 agatcggggt aggtatgagg tggtggggtt caccgccgcc cagatccccg gcatcgcctg
     1441 ccggtcctac cccccggagc tggcggggcc gctatatccc cggggcctcc ccatctggcc
     1501 ggaggaggag ctggaggaga tcgtccggga ggagggggtg gagaggtgca tcctcgccta
     1561 cagcgacctc tcagcccagg aggtgatcga cctcgcaagc cgtgtcttag cctctggggc
     1621 ggacttcggc ctcctgagcg gcgagcatac gatgctcaaa agccgcctcc cggtgatcgc
     1681 cgtcaccgcc gtcaggacgg gggcgggaaa gagccccgct gcccggtacg tctcccggat
     1741 cgtccggggg tgggggctcc ggccggcggt cgtccgccac ccgatgccct acggcgacct
     1801 cgatgcgatg agggcccaga ggtttatcgc cctcgatgac ctggatcgcg cgggggtgac
     1861 tatggaggag cgggaggagt atgaggccca tctgagggag ggggcgccgg tcctggcggg
     1921 ggtggactat gccgaggtgc tggagatggc ggaggaggag ggggacctga tcgtctggga
     1981 cggcgggaac aacgacctcc ccttcttccg ccccgacctc tggatcaccc tcgccgacgc
     2041 cctccggccg gggcacgagc tctcctatta ccccggccag gtgaacctcc gccgagcaga
     2101 cgtcgtcgtc atcaacaagg cgaggacggc gggctcccgg gccgtggcgg agatcaggga
     2161 gagcgtccgg agggttaacc ccggggcgag gacggtcctc acctcctcgg aggtccgggg
     2221 cgaccgccct gagctcgtcg ccggcagaag ggtcctggtg gtggaggacg gtcccagcct
     2281 cagccacggg gggatgagcc acggggcggg gctggaggcg gcgtggcggt acggggcggc
     2341 ggaggtggtc gacccccggc cctacgccgt ggggtccttc gcggagatct tcaggaggta
     2401 cccccagatc gggcctgtcc tcccggcaat gggctactac cctcaccaga tggcggagct
     2461 ggaggagacc atcaaccggg tccccgcgga ggcggtcgtc gtctccacgc ccgtggacct
     2521 ggccgcctcc ttgaatatca agaagcccgt cctccgggtc acctacgaga tggtggagga
     2581 ggaggagccg gggctccggg gggtggtgga ggggttcctc gccaccctcg ctgcgggggc
     2641 gagggccggg ggtgggtgaa cggtcgatgt cgtcgccttc ggcggaaacg ccctcctccc
     2701 cgaccctctc gaccccagct cccaggggtc ccgggcccgg gccctggcag gggccctccg
     2761 cctcgtcaac ccctccgggg agggggtggt cctcgtccac gggaacggcc cccaggtggg
     2821 gatgatcctc ctccggatcg aggcgacccg ggaccggatc cctcctgagc ccctggacgt
     2881 cctcgtagcc gagacccagg ggtccatcgg ctacctcctc gcgaggacca tcagatccgc
     2941 ctcagcccat ccgcatcagg acgtggcggc ggtgatgacc caggtcgtcg tcgacggatc
     3001 ggaccccgcc ttcgcccggc cgacgaagcc ggtggggccg ttctactccg aggaggaggc
     3061 tctccggctc cgggcggagg cgggctggga cctcgccccc gggtccaaag ggtggaggcg
     3121 ggtcgtcccc tccccccggc cccgggagtt ggtggagatg ggggtgatcc gggaggcggt
     3181 ggccgagggg cacctcgtca tagccggcgg cggcggtggg gtccccgtcc gccggggccg
     3241 gggccaggag ctctttgggg tggaggcggt ggtggataag gacctcacct cctccctcat
     3301 ggccgtcggc ctgggggcgg agaggctggt gatcctgacg gaggtggacg cggtctacag
     3361 ggacttcggc ggcgaagggg ccgagccgat cccccacctc cgccccgaag aggcccggga
     3421 tctcctagat ctcctccctc cggggagcat ggggcctaag gtggaggcag ccctcgcctt
     3481 cgccgaggcg accgggaggg gtgccctcat aaccgacgtc gaccatctgg ggagcgccct
     3541 tgtcggaagg gcaggaactt ggatccttcc ctgagctccc ccgggttcca cctcatcggt
     3601 tcggaccagc ttcggctgag tagcgaaggg cccgggccgt ctggccgccc ttggagaggg
     3661 ggattcagcc tgcctctcaa aaaagccgat ttttggcctc tccccccttc cccctgagcc
     3721 gggcaaaata gataatctgt cgcgccatgg tcatgagggg tcttctatcg atccggaggt
     3781 taaatcttgg aaaggtggtc ctcgaggacg gctttcatcc tcgcctccgt cggctcggcg
     3841 gtggggatag ggaacatttg gcggttctcc tcggtggtgg ggcagaacgg cgggggggct
     3901 tatctcgtcc cctatctcat agcggtcttc gccttcgccc tccccctgat gatgctggag
     3961 ctggcggtgg ggaggcggct ccggggggac gtggtcaccg ccttcggccg ggcgagccac
     4021 cggttcaggc ccctggggtg ggtcgtctgc gccgtcgtct tctcgatcct ctcctactac
     4081 ctggtcgtca ccggctggac cctagcctac gtcggcttct ccatcgccgg agagggggtc
     4141 gagttcgcgg atttcacctc gacctacagc cccatcgtct acttcatcgt ctcggccctg
     4201 gtgatggggg ggatcgtatc cctcgggata aaggacggga tcgagagggc gacgaagctc
     4261 ctgatcccct tctccttcct cctactcctg ggccttgccg ccttctcctc gaccctggag
     4321 gggtttgggt ctggatggcg gtacctcttc acccccgact tctccgtcct cgccgacccc
     4381 ctcctctggg ccgccgcctt cggccaggcc ttcttctccc tctccgtcgg ctacgggatc
     4441 ctcctcacct acggcgatta cctcgacgag gggattggga tcctgaattc gtcattgatc
     4501 ataaccttcg ccgacctggc ggtggcgatc ctggcggggc tggtgatatt tccggtcgtc
     4561 ttcacctacg gcctggagcc ggcggccgga gcggagctcg ccttctcgac cctaccccgg
     4621 gccttcgagg tgatgcccta cggctctgcc tttgccgtcg ccttcttcgc cctcctcttc
     4681 tccgccgcca tgacctcggc ggtctcgatg ctggaggtga gcgccgcctc cgtggccgct
     4741 gccaccggct tctccaggag gagggtgagc ttcgccctca ccgccggcac catcgccctg
     4801 gggctgcctt cggccctcag ctacagcagc gccgccctgg aggtcggggg ggtgagggtc
     4861 ctcgacatca tggacgagac cgtcgggggc gtaggcctcg tcctgacggc gaccctcatc
     4921 gccgtcacct tcacctggtt tatggacggg aaggccctcg ccgaggagat ggggcggggg
     4981 aggaggctgg cgctgcctat agccaagtat gcggtcccgg ccgtcatggc catgaccctc
     5041 ctggcgaggg tcctcctctg agagggcgag gaggcccctc cagaaagctc aagctcttga
     5101 aggagaacgt cctcctggtg gagaaaggag atggccagta gatggcctga ggggaggagc
     5161 ctgctaccat cctccattgg cccaggagga gcattatcgg atggatgctg gtttctatgg
     5221 atggagctgt attttgcgat ccgcccggct tctcggatgg ctcagaagcc tccaatctga
     5281 ttaagagact gataggctac gctggcagaa gcttttcttt ttggattcgg attgatttag
     5341 aagaatggaa ttcgagatgc atagacttca tcgtctcttg gatttcgatg cagagagaaa
     5401 ttcacatatt atataaataa ttaaaataat cgctaatagg cggctcgata gatatttatg
     5461 ccatcaaccc gaaggagagg tgtgcctgga cgatgaagct gaataggttc ggttctcgga
     5521 caggctggga gctccttggg ggttgtgagc ttctgccgga atagccggct aggtcgaaat
     5581 tagaccgcct ttcacggctt aatgaggcgg atcatggaaa ccatccgatc tagccatagc
     5641 tctcaggagc gagctatgcc gcatcgggcc gatttttttg cggccgtcga tggcatcaaa
     5701 ttagagggta acagcgaacg gagtgggaac tgaatgaaga gagaactggc tataacgctg
     5761 gtaatcggaa tcgccctggt gggcatggcc ggcgctgcca actacatgta cgagaagtcg
     5821 aacataaagg gcgtcggcta caagaacacc gagatcatca ttcagactca gcccggcctg
     5881 aacggaaaca agctggtcga gaaggagtct ggaagcggta acgtcatttc atcgatgacc
     5941 gagatcgagg ccgagaggga cccctatgga ccctggtgcc ccgggaccga gtgtggatct
     6001 ccctatcaat gtggtggaga tgatccctgc atcatcgata gttttgatcc cggtagcggc
     6061 tgcgtcgact acatcaactt caacaaggag accgagctgg agtacatgcc tgtcagctat
     6121 cagaccggca cctacgatca gaagtggatc gagaagctct gcgtccagaa ctactacatc
     6181 ggagccacca tggtcgagat gtacacccac gccgagcacc tccagaagaa caccgaggtg
     6241 aagacgagga gggattgtag ctcatgggat ccgacgaact gtggcgcaga cggctgcttc
     6301 ggctgcggcg actgcaccgg agccctggag gctaacatca actccaacgt catcggcgtc
     6361 gcccatatcg gctggctctc caaggatgta gccaccaact gcaaggggag acactacgag
     6421 tggggccggt ccgttgaaga cctgacgggc gtcttctcca tcgagaagtt catccagctc
     6481 tggggcaact acacctgcgg cgcaatcagc gttgactggc tgccctgcat ttaaattaga
     6541 ttaagactgt aaaaaggaac atgacctcag gctatga
//
LOCUS       DASM01000169           13952 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_93717, whole genome shotgun sequence.
ACCESSION   DASM01000169 DASM01000000
VERSION     DASM01000169.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 13952)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 13952)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13952
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_93717"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2823 a         4129 c         4407 g         2593 t
ORIGIN      
        1 gggctcgccg gcggtgacgg cgtacttgag ggaggagaag tcgtacctcg acatatcccc
       61 cttgatcatg aaccgataga tcgtcggcgg ggcgcagaag gttgtgaccc cgaacttggt
      121 ggccatctcc atcatcctgc cggcgtcgaa tcggtcgtag tcgtaggcga agaccccgga
      181 gcccgcgatc cactggccgt agatctgccc ccagacgcac ttcgcccagc cggtgtcggc
      241 gacggtgtag tggaggccgt cctcgaccac gttctgccag aacttcgcgg tcaggatgtg
      301 gcccagggga taagtctggt cgtgcttgac catcttgggg tagccagtgg tcccggaggt
      361 gaagtaggcg aggaggacgt ctttgttctt cgtcgcctcg tcctcgatcg gtctcggaaa
      421 ctccgatgag gatttctcca gctcctctct gaaattgatc cagccgggcc taccggactc
      481 ctggccgacc agggccctga ccggcttttc gccctcgacc tccctgcagg ctccgtcgac
      541 ctcgtcgggg acgccgtcct ctgcgatgga gacgatcatc ttgaggtccg ccttgctgat
      601 ccgataggcg atgtccttcg ccctcaacat atgggtcgcc gggatcgcca ccgcccctac
      661 cttatggagc cccaccatgc atatccagaa gtcgtaccgc cccttcaggg tcagcatgac
      721 cgagtccccc tttcttatgc cgtatttttt aaataagttt gccgcctggt cgctcctccg
      781 cttcagctcc ccgaaggtga ggatcagctc gtcgccccgg tcgttgcacc agacgagggc
      841 ttttttatcc ggtgccattt cggcgtagac gtccaccacg tcgaaggcga agttgaacct
      901 ctccggcaca tttatcttca tattattttt aaattcttcg taagcctcga attccgtccc
      961 tgatacgaac ctggaaacta atgacgacat atagaccctt cctggcgcgg gggctcgtgg
     1021 ccacccttcc gaccggccct cctggatcgt cagaccccgg tggaggggcg gatcatcgca
     1081 tccccgggag ccgtcgacct catttgccag agctccgagg ccgatggctc attatccgcc
     1141 tccgagaacg actccctcca gccgaatctc cgccgcctct cccccgggcc gcggatctcg
     1201 cgaccgacct ttggggggag atgatccaag gatgcggccc cgcatcgcct cccgattaga
     1261 aggggctttg atataaatga ttaatcctga gagtggtgcc gccgcccggt caccaacacc
     1321 ttttttaaaa ggctatgccg agatcgaggt gtgctactgg gcgtcctatt ctccggaggg
     1381 aaggactcgg tctacgcatg caggcgggcg atggagaccg actcggtcgc ctgcctcata
     1441 acgatggtat ccgcaaaccc cgacagctac atgttccaca cccccaacat cgcctgggcg
     1501 gggctcatag cagaggcggt cggagtccct cagctcaatt ggccgacggc gggtgaagag
     1561 gaggcggagc tctccgacct ctccgacgcc atcgccgccg cggtggccga atacggggtc
     1621 gaagggatcg tcaccggggc gatcgagtcg gtctaccagg cggcccgggt ccagcggatc
     1681 tgccgggagc tggacctctg gtgctataac cccctctggc agatcgacca gctggagtac
     1741 ctccgcctcc tcgtatcgga ggggtttgag gtgatcatca ccggggtctt cgcctacccc
     1801 ctggacgagt ccttcgtcgg cgccaggatc gacggtccac ggatcgagaa gctcgaaggc
     1861 ctccgggacc ggtacgccat caacccctcg ggggaggggg gggagatcga gacgatagtc
     1921 ctcgacggcc ccgtcttcaa gaggcgggtc gaggtcctga aggcggtgaa ggagtacgat
     1981 aattatagag gacgtctcat aatcaaagag gcgaggctgg tggacaaatg atcctcctcg
     2041 tcgacctctg cttcaggccc cggtccctct cgaggcacga gttcgtcgat cccatcgccg
     2101 ggattctgag gcgggccggg tccgagtatg aggtcgtcca cttcaccgag gtgaccgaag
     2161 acgaaatcct gagggccgac cggatcgtcc tctgcggcac cgccctgaag gacgacctct
     2221 acgcccgaag gatagacctg ttctcctgga tcagagagtt ccccaagccc gtcctcggga
     2281 tctgcgccgg gatgcaggtc atagccgccc tcttcggcgg cgaggtcgtc ccccagccga
     2341 agatcggcct cgacgctatc gatatcttca aggagacccc cctcctcggg acgccccggc
     2401 ggatcgaggg ctaccacctc cacaacttcg gcgtcgccct cccgcccggc tttgagatcg
     2461 tcgcggggcg gcccggagag gtggaggcca tcaagcacgc agataggccc gtctacggga
     2521 tcatattcca ccccgaggtg aggaaccggt ggatagtgga gagatttgat gctctccctc
     2581 tgaatgagat ccgttgaacc tccgggtgaa taaagccggt ggaggtccga tggcaaaaag
     2641 gagccttccc ggagatcatg gccgccatcg gagacgggcc catcctcaga ggcggtcgac
     2701 ttttcctgct gagggattcg ggaccgtagc aatcgaatcc atctgcctgc cttctatgag
     2761 gatctcggaa cggtccgcgt tatcaccccc cgaatatccc atcctgaaga ggacctgccc
     2821 ctggccatca gtaaatgcag aagccgtcat atccggcttc aagtaagcga atatacaaac
     2881 cttcttaaac cttctgatac ttgaagggag ggaggaggtt ttcttccttg gaggaggtca
     2941 taagatcgat aaccgaccgg ggggtccttt cgcagatcga gagggtcgtc ccctttcacg
     3001 gctacctcag caccggcgtc ttcgtcggca tccagatcta tgacatagcc cgaagggcgc
     3061 tgggattttc ggaaggggag aggctctttg tcacctgcga gacttcgagc tgcctgccca
     3121 acgcctttca ggtgctgggg ggttgcacca tcggaaataa ggggcttcga atcgagaacc
     3181 tggggaagat ggcggcgacg atctccaggc gggctccccg aggtgcggag agggtcaatg
     3241 gcaaaaggat cgtcctcgac cctgccaaga ccgcctccta tccgagggtt cactcctggt
     3301 acatgaaaat cgataaggtt cctcatgagg agacgatctc ggacctgatc agggccgaag
     3361 atgggattta cacctggagg atggtggacc tcgaggtttt agaaaagccg gagacgagga
     3421 tcgccgtctg caactcctgc ggcgaaagct tcgtccttca gggagatgag aggagctgca
     3481 aaagctgcgc tcaacgatcg aagagcaaga ttgagatcgg ttctgcgggg attagatgag
     3541 agaggtgctt ctggaagaga ggggggagaa ggcccaggtc tatcttcccg agaagtgcat
     3601 aggttgcggg acctgcgtcc agatatgtcc caaaggagag ctggtgatag gctctgtggg
     3661 ggccgtcgcc cgggggctga tcgataagga gttcctcgag aagcaaaata gcggaggatg
     3721 cgtcttctgt gctttatgcg cccgggtctg ccctaccggg gctctggagg tgagagtttc
     3781 cggcaaggtg gagaaggacg attcctacct caacttcgcc ctcagtccca ccctggtgaa
     3841 tgaaagatgc gtccactgcg gcctctgcgc cgaggtctgc ccccgggact gcatcgagct
     3901 cgaggataga cggctcgccg aagatggcag cttgaggctg gaaggaaaga cgattataga
     3961 tctcgatcga tgcgtccact gtggctggtg tgccgccgtc tgtcctgttg gggccatcac
     4021 ttttcagaag cccttcgccg gagaattctt ccgggacgat cagatctgcc aggcctgcag
     4081 aacctgcgtc cacacctgtc ccgccaacgc cctcttcaac cgcccctggc ggcagggcga
     4141 gatcgtggag aaggtgaccc accggaggga cgcctgcatc ttctgtggag cctgcgccca
     4201 ggcctgcccg gtggccgcga tctccgtcag aaagacggcg atcgttccgg agatgagggg
     4261 gaagaaggcc ttcgagaaga agcttgccac cccggctccc cggccgaccc tcacctccac
     4321 cctcgaaacg gacgaggagg cctgcctagg ctgcggaaac tgcgtcatcg tctgtcccgt
     4381 caacgccctc tccgacccgt atcttgccgc aggccacctc aacgagctgg acgagaagcc
     4441 cctcctggag gtcgaggacg gcgccgtcag ggttgtagat caggaagttt gcggctcctg
     4501 cgccacctgc agcatgatct gccccgtgga cgccatctgg cttctgagaa gggaggtgat
     4561 cccatgaccc gatcggctaa tatgatcata aagaacggat acgtcttcga tcccctaaac
     4621 gacctggatg gagaggtgat ggacctcttc atccgggatg gccgggtcgt cggtgacctc
     4681 agggccgggg agaagaagga agcgaaggtg atagatgcct ccggcaagac ggtgatgcct
     4741 ggtggggtgg actctcacgc ccacgtcgcc ggggcgaagg tgaacgccgg ccggctgatg
     4801 aggccggagg accattaccg ttcggtccgg gagaagaccc ctctcaccca ttcggggagc
     4861 ggctacacca ttcctagcgt ctacaagcag ggctacgact acgtcgccat gggctacacc
     4921 accgtttttg aggccgccat gccgcccctc gaggcccgtc acacccacga ggagatgagg
     4981 gcgaccccca tcctggatat gggagcctac ctcgtcctcg gaaacaactg gtttatcatg
     5041 cgttatctga aggagggcga catagagaag gccgctgcct acgccgcctg gatgctccgg
     5101 acccacaagg gatacgggat caagtgcgtc aaccccgccg gagtcgagaa ctgggggtgg
     5161 gcgaagaacg tagccggcct ggacgagccg aacatccact tcgaggtgac cccaagggag
     5221 gtgatccggg gcctcgccga ggtgaacgag atgctggggc ttcccatgtc tctgcacctt
     5281 catgccaaca acctggggca tcccggcaac tgggagacgg ccagggattc cctgaggatc
     5341 ccctcggggc tcgctcctgc gaacagatct gaagttgaag gtccagagac gaaattggac
     5401 tttaggagaa ggcagacggt atatctcgcc cacgcccagt tcaacgccta tggcgggacg
     5461 tcctggaggg acttctgctc tggcgccccc gaactggcca ggtacataaa ccagaccgag
     5521 gacgtggtga tcgacagcgg ctcagtaccc ttcggcccgg ccaccaccat gaccggtgac
     5581 ggtccggctc agcacgatct gtacgtcatt accggccaga agtggtcgaa tacggatgta
     5641 gagatggaat gcggatcggg ggtcctcccc ttcctctacc tcaagacgag ccccgtccac
     5701 tcggtccagt gggcgatcgg cctggaggtc ctcctcctga tagacgatcc ctggaagacg
     5761 atcatgacca ccgaccaccc caacggcggc gtcttcactc gatatccaga tcttatcgca
     5821 tggctgatgt cgaagagggc gagggacgag accgcccggg aatgccacaa gtgggcctac
     5881 gacagatcga gcctgggggg ggtcgatcgg gagatctccc tctacgaggt ggcggtgatc
     5941 acccgggcaa acccggccag gacgatcggg atcgcccacc ggaagggcca cctggggccg
     6001 ggggcggatg gggacgtgac gatctacgac ctcgaccccg ccagcctcga ctccgacgat
     6061 ccctccgcca tcaggtccgc ctttggaagg cccgaatata cggtgaagga tgggagggtc
     6121 gtggcccgcc ggggcgagat cgtggcggtc ccggaaggcc gcagattcta caccacgccc
     6181 tatgtcgatg aaggtcatga gttggagatg atgagggatg tgaaggagtg gttcaagtac
     6241 tacaccgtcg gcttcgccaa ctatcccgtc ccggagagat acttgaaaaa tccggtgcct
     6301 atcgccatca atcggcctct tgaggcggtg gtgaggaggt gatctgatgg aggcgacgct
     6361 cacccccaag ggacggatcg ggatcatgtt ggaggcggag gcgatctccc ccgacgtatt
     6421 ccgctccttg ggcataaggg agatcgaaga gctggaggtc tggcagggtc cagagaggca
     6481 tcccctggcg gagttcttcg atgtggaggt cctgggagag ggatcggggg agacgaggat
     6541 agtgatcgat ggggacgtct ctcgagtcaa gcggatcggc caggagatga cgtcggggtc
     6601 gatcgaggtc cgcggctctg cggggatgca caccggcgaa agaatggccg gggggaagat
     6661 cgccgtcgaa ggtgacgtcg gctcctgggc agggatggag atgtcgggag gcctcctcac
     6721 cgtcctcgga gatgcggggg accacgtcgg ctcggcttat cggggaagct ggcaggggat
     6781 gaccaacggc aggatcgtta tccgcgggaa cgcgagaagc cagctcggtg gagggatcac
     6841 cgggggcgag atcatcgtcg ggggagacgt ggagaacttc tgcggcatcc gccagagcgg
     6901 cgggacgatc gtcgtccggg gctcggcggt ccggggcgcg ggggcggaga tgagcggagg
     6961 caccctggcg gtggagggga aggtcgagca gatctctccg ggatttctgc cttcagggca
     7021 ggaggagaac ccgacccttg ccgaggtcga gctgatgggc aggtttgaga ggttcactgg
     7081 cgactatgcc accttcaaaa atccgaaagg atcgcttctc ctaaaggagg tctgaaaggg
     7141 tggaggccag gcttaatatc gtttatgaaa ggggaggtat cgaggagatg atctcggaat
     7201 ttttgctcaa caccggaagc accatcgaag aggggaggct tgccaagggg gggagcaagc
     7261 tgaccgagga gtacaccctg gaatgtgcgg tctgcgagat caaccttcac gactatctcc
     7321 ggctcggatc gccggagaag gtcttggtct cgacgaagga tggaaggtac agcgtcgccg
     7381 tttacgccta tccgagcgat tcggtggagc cgggacatat cttcatgccc cgggccatct
     7441 ggtcgaatgt agtggtggac tcggagacct tctccaccgg atctcccctc tacaagggct
     7501 cgcctgtccg ggtcgaggcg acggaagagg aggtcctcag cgccgaggag atcgtcctca
     7561 aggtttacat gcgaggcaga agatgacttt caaaaatata atatgtcccg tctgcggcgc
     7621 ctcctgtgac gatatccagg tcgaggtcgg cggcggtggc ataagggtca agaacgcctg
     7681 caggatggga aacgccaagt tccaggagat atcaagcccc catcggatcc gcgagccgag
     7741 gatccgggat ggtggccgga tgagggccgc ctccttcccc gaggcggtcg atcgagccgc
     7801 ctcgatcctg gtggaggcga tgcgccccct gatcttcatg ggatgtgaga cctccagcga
     7861 ggcgatggag gtggggctcc acctcggaga gcttctgggg gcggtcgtcg actccaactc
     7921 caccgtatgc cacggcccca cggccatggg gatccaggag gcgggaaggg tcggctccac
     7981 cgcgggccag gcaaaatccc gagcggacct cgccgtctac tgggggtcta accccctgga
     8041 gtcgatgccc cggcacatgt cccgctatgc ggtcttccca agaggccact ggactcggag
     8101 ggggaggttc gaccggaccg tcatcaccgt agatccgaga aggaccataa ccgccgagaa
     8161 ctctgacctc cacatccagc tcaagcccaa caccgactac gagctgatct cggcgctcct
     8221 caccctcctc cacggcaaaa ggcctcacga gtcggtggag gagattaccg ggatctccat
     8281 ctcccggatg gaagagatgc tggagatgat gaagtcgtcc aacttcggcg tcatctacgt
     8341 cggcctgggg atcgcctcct cctatggaaa gcaccggaac gccgaggctg ccttcaacct
     8401 ggtcaaggag ctgaacggca ccaccaagtt cgtcatcggg gccctgaggg ggcactgcaa
     8461 cgtcgcaggg ttcaaccaga tcgcctccta tctctacggc taccccttcg gcctcgactt
     8521 ctcccggggg tatccccgct acaaccccgg agagttcacc gccgtcgacc tcctccggga
     8581 aaaagacgtg gacgccgctc tgatagtctc cgccgatctc gtctcccact ttccggcgga
     8641 ctgcgccgaa tatctcgccg agatcccggt ggcctgtatc gatatcgctc ccacccccac
     8701 caccatcgtc tccgatgtgg tgcttccagg ggtcatagac gccatggagt gcgacggtac
     8761 cttctaccgc ctcgacgacg tgcccatcta cttcaggccc ttcaccacct cccccttctc
     8821 cttcaccgag agcaacgagg ataccatgcg aaggatattc gatcgggtaa gagagagcct
     8881 gaagtcttca aatctggccg aagaataacc ccttacttcc ggaggatagg aattgtctac
     8941 aaaactggtg ccatctcttt gcccttactg tgcagtaggt tgcggctatt acctcgttgt
     9001 agagaggggc aaggccatgg gcgttgagta tatgaaaggc caccccgcct gtgagggagc
     9061 cctctgcccc aaaggcaacg ccgtcctgga gatcttgaat aacggggaca ggctcgaata
     9121 tcccctgaag aggtatggcg acgaatttgt gaggatctcc tgggaggagg ccctggatct
     9181 ggcagcaagg ggcctggccg gcagcgcaaa ggaccacggc ccggaatccc tgggcttttt
     9241 ggcctcatcc cgctgcaaca acgaagagaa ctacctgatg cagaaactag cccgcctcct
     9301 cggctcgccc aacgtagaca actgcgcccg cctctgccat tcgcctaccg tcgtcggcct
     9361 cggcgcggtc ctggggacgg gagccatgac caacaacctc ggagacctca aaaactcaag
     9421 ctgcatcttt gccataggca caaactttgc cgaagctcat cccgtcgtct cgcgctgggt
     9481 agagaaggcg aaggagagcg gggcgaaggt tatcgtcgcc gatccgcgga tcacctccac
     9541 ctcctggatg gcggacctcc acctgcggat caaccctgga agcgacatcg acctcttgag
     9601 ggggatgatg aaggtgatcg tggatgaggg gctcgccgat gagaggttca tcgaagagag
     9661 gactgagggc tttgagatcc tcgctgccag cctcagaggc tattccctgg cgaaggcctc
     9721 cgagatgacg ggggttcccg ctgaagagat ctcccaggcc gcccggatct atgcccggtc
     9781 ccccgcttcc tctctcctct actccatggg cataacccag cacatctgcg gcaccgacaa
     9841 cgtgaagacc tgcgccaccc tcgccctgat cgccggccag atgggaaggc cgggggccgg
     9901 ggtctggccg atgcgcggcc agaacaacgt ccagggaaat tgcgatatgg gcggcatggc
     9961 cgagttttat ccaggctaca ggagagcctc tgaccccaaa tcgatcgact ttttcaaggc
    10021 tgcctgggag gcggaggacc tccccctggg gccggggctg acctctacgg agatgacgga
    10081 ggcggcgctg gaggggaaga ttaaggggat gtacatcata ggagaagatc ccgtcacctg
    10141 cgatgccaac atcaacaaga cgaaggccgc cctcgataac ctcgactttc tcatcgtcca
    10201 ggagatcttc atgaccgcca ccgccaggat ggccgatctc gtcctgccgg cggccgcctg
    10261 ggcggagaag gacggaagct atacctccat ggagcggcgg gtccagtgga tcgaccaggc
    10321 cgtccctccc gtcggcgagg cgaaggaggg gctatggatc ataaatgaga tcgggaagag
    10381 gatgggtctg gacctgaagg gggagagcgc cgatcaggtc ctcaaagaga tcaacaggaa
    10441 cgtcccacaa tacgggggga tgacccggga gcggatctcg aaggtgggtg gcctcctctg
    10501 gccctgcccc gacgagggcc atcccgggac tgagatcctc catcaggaga ggttctccac
    10561 ccccagcagc agggcgaata tcgcctccgc ggataacagg ccccccgccg aggagacgag
    10621 ccctgagtat ccgatcctcc tctccactgg aaggatcgtg gtccactaca acagcggctc
    10681 catgacgagg cggagcccct ccctcctgga tagagaccct gagctctacg tcgagatcaa
    10741 tccagaggat gcagcgaggc tcttgatctg cgaaggtgat atggtcaagg tggagacggt
    10801 ccggggtgag gccgaggccc gggcccgggt gacgaaaaga gtgaagccgg ggatggtctt
    10861 tatgcccttc cactggcagg cgaccaacat catcacctcc gacgccctcg accccgtatc
    10921 caagattccg gagtataaga tggccgcctg cagaatatcg gtgaagcctt gaggcccgag
    10981 ggatgaagga gagctggcac gtcgagaggg tcgaggaggc ggaagagttc tggaggggcg
    11041 agcggctcga tcggggcgga gcctttcaga cggtgaggtg cactgagata tcggggtccg
    11101 agaggcggag cctggaggtg gacgtcgcca tcgaggagag ggttgccctg atcctggatg
    11161 gcatgatggt ggcggagctc ttctgcctcc cttcccagct tgaggagctg tccgtcggcc
    11221 atctcctctg cgaaggccac ctccgtggga tcgaagacct cgtctctgct agggtggctg
    11281 gcaaagagat attctgtgag ggtagaggcg gtgggatgaa ggtggaggct cgtcctacgg
    11341 cctcagacct gaggatcagt cccgaggcgg tctttaaagc cctcgacatg atcaacgacg
    11401 ccgctctcgt ctggaggagg accgggggca cccactcggc tctggtcctc ggagaggacg
    11461 gagccttaag ctcattctgc gaggacgtca gcagatcctc ggcggtggac aaggccgtcg
    11521 ggtctgccct catggcggga gccgatccgt cgaggtctgt tcttataact acgggaaggc
    11581 tctcatcggc gatcgtctcc aaggcgggga tggccgggat ccccgtcctg gtcagcaggg
    11641 caggcccctt gaattccgga attgagcttg ccgagaggtt ggggatgacc ctggcagcct
    11701 tcgcccggag gccaaatctc tatgtctacg ctggcgagga gcggatatta tgacgatggc
    11761 atcgagctat tttgaattta gggagggagg gcgagaatga agggcgaaaa taccgggatg
    11821 acggatcttt tagaggtcgc aagattgcgg ttcatctccg agctggaccg ctggtgcgac
    11881 cagagggacg ttccgagaga tgagctggat gaccaggtgg tagcgacccg gcccctcagc
    11941 agcactgaag cgatcggaaa ccccgagatg gacgacttcc ccctcctgag gggaaaggag
    12001 gttctgatgc aggcgaatta tcgggggtca tgcggccagg ccttcacctc cgcccggggc
    12061 gtcttcgccg gaaccctgga gggggtgctg gagctctccc tggataacac cttcgagcgg
    12121 gcggtcctcg tatcgacgat caacgccgtc atgagatatc tcggcctctt ggaggggacg
    12181 gtccactgca gggatgccgg cccccgggaa tgtgccgaaa gcctgggcca gctcttgagg
    12241 ggagagaggc cttccagggt ggggctggtg gggcttcagc ccgccatcct ctcgtctctc
    12301 gtcgaggtct tcggtcccga gagggtgatg gtctcagacc tcgccagtgc cggtgaaacc
    12361 cgttcggggg tcaagatcct ggacggagcc cggcctgagc ccctctttga gagttgcccc
    12421 ctggttctga tcacaggcac caccctcgta aacggcacca tagacgccct tctggagctg
    12481 gcttttcggt tcgaaaaccg ggtcgtcttc tacgggacga ccattgcagg ggccgcccgc
    12541 ctcctgggcc tggagaggtg gtgcgtctgt tcagagtgac cttcctcatc cagaaacctc
    12601 ctcttaaaat ttatagaacc actctcagga gcggttgaga agatgtctga gataaccctt
    12661 accatcgacg gccgagaggt gacggtcctt tcgggggcta ccatcctcga tgcggcccgg
    12721 aaagccggct cttatctgcc ggcgctgtgc catcatcccg acttgaagcc cataggatcc
    12781 tgcaagctct gcatcgtggc agtagaaggc ctggatcact atcccacatc ctgcaacacc
    12841 ctcgcagaag aggggatggt ggtaaagacc tggacggagg agctgcagga gatgagaagg
    12901 cacaccctgg aggtgctcct ggccctcacc aaccacccca cctcatgcct cttctgcgac
    12961 cgaaaggacg aatgcaccga cctgcgggag tgcatgagga agtttccggt gactgtgggc
    13021 tgcaagttct gcccccagga tggctcctgc gagctgcagg aggcggtgga gttcgtgggc
    13081 ctggagaagg tcagatatca gctccacttc aggaatatgc cggtgttgag agagcccttc
    13141 ttcgatcgga actacaacct ctgcatcatg tgcagccgct gcctccgagt atgctcggag
    13201 gtccgggggg agggggtcct caagagcaac cccgactttc accgcatgca ctggataggg
    13261 ccctcatctc ttttggactc cgattgcaag ttctgcggcg cttgtgtcga cgtctgcccc
    13321 accggcgcct tatccgtccg gtccgataaa tgggcgcggc cggagaaaac gatctccacc
    13381 gtttgcccct actgcggcgt cggctgccgg atgaagctcg gggtcgaaga cgagaggata
    13441 gtcaccatca ctcctgatcg ggagggaccg gtgaatcggg ggcagttgtg tgtaaaaggc
    13501 cgcttcggcc tcgacgagat cgtccatcat cccgaccgca tcaccacccc cctgataaga
    13561 cgaaatgggc gattcgagga ggcgaccctc gaggaggccc tcgacctcgt cgcgagaagg
    13621 ttctccgaga tcagagatga atacggcccc gactccctgg gagccctctc ctcatcccgg
    13681 tgcaccaacg aggagaacta cctggtgcaa aaatttacaa gggcggtgat gggcactaat
    13741 aacgtcgacc actgtgcccg tacctgacac gcgcctactg tggccggttt ggcccaatca
    13801 ttcggaagcg gcgcgatgac caactccatc aatgaaatta ccactgacgc ccagctgatg
    13861 ctgctgatag gcaccaacac cactgaggcg cacccaatta taggttataa aatgcggcag
    13921 gccaagcgca acggcgccaa gcttattgtt gt
//
LOCUS       DASM01000170           16462 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_3365, whole genome shotgun sequence.
ACCESSION   DASM01000170 DASM01000000
VERSION     DASM01000170.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 16462)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 16462)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..16462
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_3365"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2857 a         5324 c         5306 g         2975 t
ORIGIN      
        1 ctagttctct cttcaattaa tccctccaat cgatcctggt gatgaagaat ataaaaactt
       61 ttacatcgcc ccctcaattt tgatcggagc ttgagttggg gcatcttcgt tataccccca
      121 atttatcctc gtctctgaag cccgccccgg cctcgaaaaa gtattaaatt aaaaaatata
      181 tattataaat atatattcat ctggaagctc ttccgccata agaaggccga atgaatgtaa
      241 gaatatacaa aacttccaac gataaaggaa atctaaatag tatcggggct cctctcgacc
      301 ccttaaaacc ggcagaaaat ctgcgcgacg gctatagcga cctcctcatg atgatcgccc
      361 cttcgccgtc ggggtagtag ttcttggcgg tggtgaagat ctcaaacccc aggtcgacgt
      421 aaagcccctg ggcccgcttg ttcgtcgccc tcacctccag gatgacatcg acgaccccga
      481 gcctccggaa gatctccaga agctccgtca agaggcgtct gccgaccccc cgcccccggt
      541 atccggacct caccgctatc cagaagaccc tcccctccag gggcgactgc ctgaacccca
      601 ggaccatccc tacgatcctg ccgccctcct ccgccaccaa gaaggtggtg gggtagttct
      661 cgtagaagta ggtgagaagg tacgggtcat gccctccccc cgcctcggcg tccaaggcga
      721 ccgcctcgga gtagtcggga ggctcaaacc tcctgatctt tactccgccc tttatcttcc
      781 caccaacttc catctctttc ataccgggca actcagccct gggcctcaag ataaaaatag
      841 cttaccgacg gggaggtttt tatcccatcc agtgatggtg ggacggagag gcgatcctgg
      901 aggcagccgc ctcctctcgc cgatatggag ccctccggga agggattggg gggctattgg
      961 gagacggtgt cgatatgaac ctggatgcgg gtcttgggat tctggcggtt ctgatcctca
     1021 ccctcggtca gggagaggca gctctatcat cggggctgga ggccgtcagc ttcaacggca
     1081 ccacctacct cctcgcgaag tccgaggcgg aggtgatcgt ccccgcaaac ggcagcagct
     1141 tcaacctcac ccttcccatc agggctgata tcaggctctt caactcctcg ggaggggagg
     1201 ttcccgcgga gacgagggtc gatttctggc gggggtacta caactatcag gtcgtcgccg
     1261 gggaggaggt ggacgggcac ctaaactaca ccctccccat cgcagaccag aggttcgtcg
     1321 tcctcgccga ggagggctcc ggcctccggg tcgtccttcc cccggggtac gccaccggcg
     1381 atcggctcct ggggagggca aggcccaccc cagaccggat agagtacctg gataacagga
     1441 cggtcctcat ctggacggtc ctcgaaggga gggtgatcga cgtcagcttc tatgcggagg
     1501 atgcgccccg ggccttcagg ctcttcctcc ttctcctcgc cttccttgcc ggggtcctgg
     1561 ccctggagca tcgggcgagc atgaggaggc tcaggtccat caggcgggac gccgatgagg
     1621 atctcgaagg ccggggccgg acctagaccg aaaagccccc ggatcgaggt cagatctaag
     1681 tatcaggggg ttatccccct caccatggtg ccggaagaga tcgggaaggg cgtatacgtg
     1741 gtggggggtc cggggatttc ccatcccgcg gactgctccg tctacctcgt cgatgcagga
     1801 tcggagctgg tgatgatcga cgctggagca ggccccgggg cggaggcgat cctgagaaat
     1861 gtccggtccc tcggctttga cccctcccgg atcggccacc tcatcgccac ccactgccac
     1921 atcgaccaca tcggagggat agccaggatc cgggatgaga cgggatgcct ggtgatctgc
     1981 tccgaagcag atcgggacgg gatcgagctc ggggagccga ggctcaccgc cgctgacctc
     2041 tacggaatcg agtactggcc cgtccgggtc gacctccccc tcgatgggga ggaggaggcc
     2101 ctgaggctgg gggagcggga gtttcggatc atcgccatcc ccggccatac ccctgggagc
     2161 atcgccgtcg tcgtcgacct ggaggggctg aggttcctct tcggccagga cgtccacggc
     2221 cccttcagcg acgcctgggg atcggacgtt cccctttgga ggaggtcaat ggagcggctc
     2281 ctcgatctgg aggcggacgt cctctgcgag gggcacgccg gggtcttcag gggcccggag
     2341 gtgagaatct acatccagtc ccagctgggg aggtatcggc acctctgagt gggatcgaag
     2401 cgacgctcgc gacgggaacg gccgatgcca aattcagagg tccgccctga aggtggccac
     2461 atccccctcc gtgacggaga taaccctgtc gaggaaccta gacgcggtcc agatgttcgt
     2521 cgaggcgtgg ctcgtcacct ccggggcggt gtaggatcca cccgccaggg cgaggtaggg
     2581 gacgagctgg tcggcgaggt ggcggtcgac ggcggccttc gaagccagct gtgccaccag
     2641 ctcctccgcc gccagggagc ctaccctctc cgccggaagg ccccgctccc ccagggcgct
     2701 acctcccttc caccccgacc agagggtgat accgctcccg gtggacggga gggccctcgc
     2761 ctcggcggcg atctcggcct caaaacccgc cctcctcaga actccgaccg ccgcctccgc
     2821 ctgcctctgg acgacgtgct cggggaggtt tgacgagtgg gacacgccct ccaccgcccc
     2881 tgccactggg gcggtgaggt cggccctcac cagctccccg gggaccgtct ccagggtgac
     2941 caacccgccg ccccggggaa agtatccccg ccgctccacc gtcaacctcg cccggactcc
     3001 gaacgaggcg aaggcgggga gagcgaccct ggagaggtgg tcgatggtag gagaccacct
     3061 gacgtcggtc cccccccgga cggtcaggat cgcgggcccg tcggccctgg ccagggccgg
     3121 gaggaggcag tggatgagaa gggagacgct tccggcggtg ccgatctcaa agtcgtagac
     3181 cccgccccgg acccttccgg gcgagaagag aacctcccgg gagccgggcc tcaccccctc
     3241 cgtcctggcg tccgagatct tggccagggc ggatatcgcc gtcgagtgct ggggggagag
     3301 ccccggattg ggcctcttct cccggatcct cgtgatccgg accggctcgc cggtgaccgc
     3361 cgagagggcc accgccgtcc tgacgatctg gccccccccc tccccggagg agccgtcgat
     3421 atcgatcatc cgaccgcgga tctgaactcc tcttcgaact cctcaaccat cccggcgatc
     3481 ctggaggcga cctcgttcag gaggaggacg gggtcgacgt ccctggtcct aagccggaag
     3541 atggggtttg tcatcatcgg aaacttggcg tcgtaggtgg cgatgaggac gcggtcgtcc
     3601 tccagaagct ccgtcctgag gaggttcagg agggtgtggc tctccccaaa gaactccaga
     3661 tggagctcgt catcggtctt cttcaggacc tttacgttca tctgatcacc cccgtcccga
     3721 aatcggtgct cagcttcctc gactccacct gctcgcattc ggggcagacg agccgcttcc
     3781 cgtcgagctt caggggggag gcgcaccttc cgcagtacgc cttgacgacc cccaggtcct
     3841 cgtcgacgat ggagaggccg atggtggggg tggcgtcgat gatcttcgcc ttgacgatgt
     3901 cccggaggcc caggacctgg cggatctcct tgacgtaaga gctcttgacc ctggatatgt
     3961 ggaggagggc ctcggtgttg aatatcggcc tcgcctcgca cccccgcttg aaggcgaggc
     4021 ggaggatcgc taggctgtct ttgaggtcgg tgatctggcc caccaccacg tccccgttct
     4081 tcagcttcac cggggcgttg gccaggggat ggaccttcgc cgacctcctc ccggcgtcga
     4141 cctccagaac ccccgtgctc gaggagagaa ggtgcccccc tcggaggtag gtcccttcgc
     4201 cgggagaaaa ctcctctgaa gccccgacga cgtcgccggg gatgacgata ttcgatggca
     4261 tctttcggtt cacctgcaag atatccgata cagctctact tcaatgacct tgacccctcg
     4321 ggtgtggaat ttataggtcc ttctcagggg aaaggggatc ttctggacct cttcgacctt
     4381 acagggttct acgaaccgtc taatgaaccc ttcgctccca gcattgtgga tcgagtatac
     4441 gacctcggcc gtctccaccg ccttgcggag gaaggcccgg tcccccaggc tccccctctg
     4501 ggccccgaag ggggggttca tgacgacggt ggagacgccc ttgagggcga ccgtcgccac
     4561 gtcccccctg acgaactgga cgtcggcccc gaggcggagg gcgttctccc tcgcgatcct
     4621 gaggggcgag gggtccagct cgaccccgac ggctctcccg ccgaggaggg cggcgcctat
     4681 cgccaggacc ccggttccgc accccaggtc gcagacggtc ccctccaggt ccccccggag
     4741 gagggcggag tagaggagct ccgaggcgac ggttgagggg gtctggtact gctccaggct
     4801 ggtttcaggg gagctgaacc cctccagccc ctccaggatc atctctagct ttcttttctt
     4861 catggtctct cgggaggtca ggcggaagag agccaaaacc ccaaaaaggg cgggtggcct
     4921 tcgactcccc tctctcgggt ccggccctat atcaagtagt gggtcaggtc caggaggatg
     4981 aagaggaaga gggcggcggc gaggagcctg agccctctca gagccagggc cctgaggagg
     5041 ggcctgtaag accacctctg gagcctcggt gtcctcacct ctccccggcg gaaggcgagg
     5101 tctccgaggt ctcggccgac tccgctggcg aatctggcga ggaaccttag gggatctctg
     5161 ccattggccc caaaggcggg gaggtcattg cagccccctt tagtcagcga ctcctcggct
     5221 gatgggagag caagggggat cggcggggcg gcaccctgcc cctcccctcc atctctggct
     5281 ccctcatccg attctccggg cgaagggggg cggtttctcg caacttctgt cagccccagg
     5341 ggttcagctc cccccgcctt cgaaggcccc cccttaaagg agaggacctc ctcgatgggg
     5401 cggatcaggg gagggggtcg gggaccggac ccctctggag gccggatggg ataggatggc
     5461 tcgtcgccgg cggccgtctt cccatcggcc tgaaggaggg tcctcggctt ctgcctgccg
     5521 aggggggtct cctcgatcag gttcatcgac tcgttccagg tcctcaccgt atcgaggagg
     5581 gacgcctccg tcagagccgc ccgggtgagg acccgcccca tgaggaggag ggttcgctga
     5641 taatcctcca ggaggaggcc ctgtccgtcc cttccgctcc ctgcggcctc ctcaagcctc
     5701 gatgccatca tccgggcctc ccggatctgg aaggggctta tcatcccgag gtcgggggcg
     5761 gcgagctcaa aggcgctctc cgcccgcttg aggtacgcca tcgtctccga gaggtccgcc
     5821 ggaggctcgc ccttgaggat agaggcgatg aacctcccca gggtcttgaa ggcggggagg
     5881 agctgcccgc aagcctcctt gcagccccgc cccgccgagc cgagctgaag gatcgcctgg
     5941 gtgaagtgct cggtggcgtc ggaggcgacg tcggagagct tccagggctc ctcttttatc
     6001 agggacagcc gccaagtctc cttcatcccc atcatgctcc gccgcagagc ctcgacggag
     6061 ggcctctcag aggagtcctc tgcccgggag agggcggagt tgagggctgc caccccctcc
     6121 tccgccagct tcacccccat ctccggctcc gtctccatct ccaggatgac gaagctggag
     6181 gctagcctcg cgatccccgc gtccaccccc aggtcgggga gggccacccc cggggtcccg
     6241 gcgagctcat cgaggcactc cgccgccccg gcgtagtatg atgacgcctc gatgaagagc
     6301 tcggttgctt ccgggccatc ctcgtcgccg ccgatggtgt ggagggcctg ctggtggagg
     6361 tcccgaccac ggatcgcccg gacggcagag agccatgata cggcgttcgc ccggacgggc
     6421 tgcatgttct ttcggatgag gtccaggctc gtctccaggt ggtcggcggc gaggtcgagg
     6481 tctccccggt cgaggtggag cctcccgaag tttgcgagga cgaccccggc gttgtggcgc
     6541 tggccgatct cctcgaagat ctccatgctc ctgtggtagt agccgatggc gacgtctctc
     6601 ttctcgatct cggcgttgac ccgccccatc cggttgagga tccaggcgac ctgcctctga
     6661 tccccgagct gctcgaagtc ggcgaggctc ttctcgtact cctggagggc gaggtcgtac
     6721 tctcccatct cggcgtggat cctgccgatg ctccccgtca tctgggcgac gccggactcg
     6781 tcctcgagct ccctgaagat ctccaggctc ttgcggcagc tctcgagggc ctcctcgtag
     6841 ctctcgctgc tccggtaggc ggaccccatg ttcccgagga cctgggcgag gctgccggag
     6901 tccccctggg acgagaaccc ctccgccgcc ttctcgtagt agtggagggc acggccgagg
     6961 tagccctggc gggcgtggac ctggcccagg ttgttgtaga ggacgctcct gacgtgctga
     7021 tccgtcgtct gggatagggc cttgttgtag caccgggcgg cgatccccag gtggcccgcc
     7081 agcctgcaga tgtctccgag cctctttatg tcgtagatgt ccgagaggca gttcaacagc
     7141 ctggtgcaga tcttctcgtc gatctgcccc tcctcgaagc tctccaggag ggcggccacc
     7201 agatcagcac aatccccact tatcgatttt ggatcaatag ggcgggagaa cccccccctc
     7261 atgtaggact cgaatatctc ctggaggacg acgagctgat ccatcggcta tccatcgact
     7321 tttatcttta agtagtttct gcgggaatat tatactcagg atatattgag ccgcaaccct
     7381 gggagcggat cgaagcaatg ggggtcccgt tgaaatgagg gtcactctcg ggatcggtta
     7441 tcctagtctc ggggatatcc ccggcgaaaa ggagggttat gaagatcgat ctgatacgcc
     7501 gaggcctggg gcggccctcc ctcccccgag gcggaggacc ggccgtcgga gagccgcctt
     7561 ccgccttgcc ttcggggcag ggcggatggg ggatgggcgg caaaggaagg tggatgatat
     7621 gaaaggggga ataggcgtga tagagctcga tgagatcagg gaggctgcgc tgctgctgga
     7681 ggggagggtg atccggaccc ccctcgtccg gtcggatacc ttctcgagga tggcgggagc
     7741 agaggtgcac ctgaagctgg agagcctcca ggccggcggc tccttcaagg tgaggggggc
     7801 cgcctacaag ctcctcaaga ggcgggagga gatcggcccc ctggggatcg tagccgcctc
     7861 tgccggaaac cacgcccagg gagtcgccct ggcggcgagg gccgcagcca tcccggcgac
     7921 gatagtgatg ccggtctggg cctcgatggg aaagcaggag gctacaaaga gctacggggc
     7981 ggaggtgatc ctagccggcg atagcctcgc cgagagcata tcgatagcaa gagagatggc
     8041 agaggagggc cggcccttca tccaccccta cgacgacccg gatatcatcg ccggccaggg
     8101 gaccattggc ctcgaaattc tggaggacct cccggacccg gatctgatcg tcgtccccgt
     8161 cggcggaggg gggctcatcg gcggcatcgc ctcggcgata aaggcagtaa ggcccgagac
     8221 ggagatcgtg ggagttcagg ccgccgcctg cccctcggcc ctcgaagccc tccgttccgg
     8281 cgagagggtc accgtcgagg cgaagagatc gattgccgac gggatctcgg tgaggcaggt
     8341 cggcgaggcg aacttccccc tgatccggga gctggtctcc ggagtggtcc tggtggagga
     8401 ggaggagatc gcctcggcag tcctggccct cctggaaagg aagaaggttc tggcggaggg
     8461 ggcgggggct gctcccctgg ccgccctcct cgcctcgaag ctcgccttcc cgctggggtg
     8521 taaggtcgtc ctggtgatca gcggcggaaa cctggaccct cccctcatgg agagggtggt
     8581 caggcagggg ctcttgaagg aggggaggat catgaggttc tcggcctgca tcgaggacgt
     8641 tcccggcagc ctcgcccgcc tcctcagcct ggtggcggag aagggcggaa acgtcctgca
     8701 catccatcac gccaggggtg gagaagagat ccccttattc aagacgaggg tcgacctgga
     8761 ggtggagacc cgggggcgtg gcatgatcga ggagatcaag gtggcgctgg gagaggcagg
     8821 ctacgaggtc ttggtgaggg agtgaatggt cttttcttca tcctgcccct ctgggcgcca
     8881 tcactccgat ccgatagctt cgacggggtc gaggctggag gccctgatcg ccgggtagag
     8941 ccccgctagg aagttgaggg ccagggcgaa gaggatggag tagaggacca gatccggccc
     9001 caggtcgaag gagaggggga cgccgccgaa gccggagggg ccgaaggtgg cgatcagatc
     9061 tccggccaaa agtccgaaga atgccccgag gacgccgccg gggagggcga ggaggaggtt
     9121 ctccaggagg aatatcttca ggatcgaggc cctatcggcg cccatcgcca tcaagatccc
     9181 gacctcccgg gtcctccggc ttatgatcat gatggtggtg ttggcgatga cgaaggcgga
     9241 gatggcgagg atcatggagt agaagatcag gtttatcctc gactgggtcc ctacgaacct
     9301 cgcgatctcc cggctgtact cctgccagga gctcgtcctc cggtggaggc tccgctccag
     9361 ggcgacggcg gccgccggcg cctcctcgaa ctccgatagc cggaccccga tctcggagac
     9421 tacgtccccc tcccccacca ggtcctgggc tgtcttgagg tggaggtaga cgaggctttc
     9481 gtccttcacc gtccccttct ccaccagccc ggccaccttc agcctcaccg accggtcctg
     9541 gaggacgagg tcgaagctct cccccggccg gatctccagc ttctcggcga gcttcacccc
     9601 caggaaggcc gacctccgct tgaagttcag ctcggagaag ttcccggaga gggctgaggc
     9661 ctggaccccc atcaggaggt cctcggggcc gggatctacg ccgacgaact cgaccccctc
     9721 gacgttatcc ctgtggcggg cagccccctg gccgacgagc ctcgcggaga cggcgacgac
     9781 cccggggtgc tcccagaccc gctccgagag ggtcctgtag aggcggaggt ggtcctctcc
     9841 ccttcccggc tggacgtaaa ggtgagggtt cttctcgatg gtgttcgcca cgatctgggt
     9901 cctcaccccc tcggtgaggc cgatggacat gatgatcacc cctacggcga cccccaccga
     9961 caggagggtg aaggccgtcc ccctccggtt ggagaggatg tggcggaccg ccacggcggc
    10021 ctcaaacctg agggccaggc agcggcaagc gaacgcctcc tcgacgaagg tcggcctcca
    10081 gagcccccgc cccttcatcc ccgattcgga gcccttgagg gcctcgacgg ggtctaggct
    10141 ggcggcccgg agggcggggt aggcgccggc gaggaggctg agggctaggg cgaaaaggac
    10201 gtaggtggga aagtccttcg ggtcgatcac cagggggatg gagctgagct ccctcccgcc
    10261 cgccgtcacc tcgaacctga agcttccgag ggcgaagatc cccaggagcc ccaggccgga
    10321 gccgaggagg ccgccgagga ggcctaggat cccgctctcg tagatgaaga tcctgaggat
    10381 cctcccccgg tccgccccca gggccaggag catccccatc tcccggacct tctccaggac
    10441 gagcatgttc atgatggcgg cgatcccgaa ggcggcgatg atcatgacga agaagatgga
    10501 gagggacctc cagaagccgc cgacgttgat cgaccggagg atctcggggt tgttctcctg
    10561 ccagctggcg gcgttgtagc cgaggaggtc gatggcggcc gccacctcct cagcttcgcg
    10621 gatatcgtcg aggcggagct cgaccgaggt gatgacgtcc ccctccccga ggaactcccg
    10681 ggcggtcctg agggagacgt aggcgatcct ctcgtccagg ggggttccgg tatcgaagat
    10741 cccgaccagc tccaggtccg tcgtcctggc ccgggggaag ctcaccacca tccggtcccc
    10801 gaccttcagc cccagctcgt cagcgagctt gaagcccatc accaccttct tcccgtccag
    10861 gatctcggag aaatccccgg cgacgatcga ggagctgatc ttgtagatgg cgtcttcttc
    10921 gggggggtcg atcccccgga ggagggcgtt tctcctcttc tccttgaagg agaccgtcgc
    10981 ctgggtcgag agggtcgccg aggtggagct caccccctcg atggcggagg ccctcgccag
    11041 gagcccccgg tagaggtgga tgtacttctc cccctccgcc ggggagatcc ggacgtgggg
    11101 gagcttctcc tcgacaatcc cggtgaggag ctcctgggag ccgttcccca gggaggtgaa
    11161 gaggagggag atcgagacag ccagggcgac agccgtcacc gatagaatcg tctggcgtcg
    11221 tcgggatgtg acgtacttgg cggcgatgaa gagctcaagg ctgggggaga gaggtgacaa
    11281 gcaagagact atttaggctt cacgggttta aagccgttct cgcccccggg agggggtaga
    11341 atgctggatg agggcctggc ggcgctggcg gaggggcgaa gaggcggtga acggcggaga
    11401 cgacgatgat ctcgaagacg accaggaggt agaggacgga gagaggaatc gaggatcaag
    11461 agaggaatat ttgaactgcg gaatatttaa ctcctgcgac gacagcaaca acatcaccac
    11521 aattatttct gatggcgtag atatcagcag gcgggatctt gagatgcatc acgagaaact
    11581 gacatataag ggcggggagg cggacggcta cgtcgtcccc ctcggccccg caaacctcgt
    11641 ctttgtggtg ggggagaggg ggatgatcgg ctgcggagcc ttcgacgtcg ccgccctgga
    11701 gaggtttcgc taccctgcgg cgaaggtaaa gccgacccgg ggcccctcca tcgccgacct
    11761 cgaggacctc ctggccgggg aggtgaagga cgcgaacgga gccgccgcaa gcctaggggt
    11821 cgccgtcggg atgagcggca aggaggccct agacctcctc tgaccccctc ctcgaggagg
    11881 ccgggggcag gatctcccag ggccgtctca gcggtcttgc ccgccggatc tggcaatggc
    11941 aatggcaatg gcttcgataa cgtccttaag ctgctccttt ttgtctctca cagcggcacc
    12001 gcctcccaaa ggactcgctc tttgatacgc ggcttgatcc cgcgttcgct taccacgtcg
    12061 accttgcagc ccaacagctc ctccagatcg gccaggagtc cccccggaaa gaaggggggt
    12121 ccggccaggg ccgacgtcaa ccagaagatc gacgtcgctc tcctcccctg cctcaccacg
    12181 gcttgcggag ccgaagatcc tgacattggt tgctccgtgc ctggctgcaa tctcgaggat
    12241 ctcatccctc ttggatttta acaggcgctc gatctccccg atcttcttgt cgacctttgt
    12301 gacgcgagct tcgcccattt tgtagacctc ggcataatat gagaagttga aggcataaac
    12361 agccctctga agttctggtc ccaaaaaagc cgtctcgtct tttaatctcc cccggcctct
    12421 cgcacccggg ggctccgacc aggaggctcg gccctggggc gttgccaaac ccccacgggc
    12481 ggcggctggc gccgatcggc gcccggcccg aaggcgcggc tcggtccatc agcgtatgag
    12541 cttgcgggcg aggacgacga tgaaaagcgg caaaagcaac catcccaaaa tgccttctat
    12601 aatatctcat tcagaaatcc agtcttctga tttgcctgtt attaagtatg taatcgcata
    12661 accgatgata aatagagaga tccaaaatag aggccacctc aaacttttag gtgtatcttt
    12721 gcttcacccc agttgatatc aagcatatta aagcttgaga attttattca aattgctgcg
    12781 gcccaccccg cagtagagct gcggggtatt cgttaaaaaa atcaaaggac actatgcaga
    12841 agccttatcg tcgagaattt aagctctccc ttccgaaact cgcacttccc ctccaccgtc
    12901 accttctccg gcagcgtcga aacttgcgcc ctgaggatca ggtccgccag ctcctcctcg
    12961 catcacctta gcgatcccca cgatggagat ggcgggccgg gggttgacgt ccaccacccg
    13021 ggggaggtct ccggcgacga ggtcggtccc ggcgcagacc catgaatagt caagagcaga
    13081 aggtagaact ttaactcttc agctctacgg cggaaaccgc ctccccacca tttcccgcac
    13141 ctccgcaagc cacctctgcc tctcctccgg ggtgctggtc accaccacgg aaaacgtcct
    13201 cctgcggacg tcgttgacgc cgcagagccc gaagatgcac ctcttccaga gcgtctccag
    13261 ggggtcgccg aaggcctctc gctcccgctc cggtggagtg ttggaggtgt tgaagacgag
    13321 ggcgcactta gcccgcagaa ggccgaccgg gaccccctcg ccgccgtccc cctcaacgaa
    13381 ctcgtaggcg acgccgggcc gtatgactcg atcgacccac cccttgagga tcgccggagg
    13441 ctgcccccac cagttggggt ggacgatgac gatcccgtcg gcggcggcga tctcgtcgca
    13501 gtggctcttt atagcgggat cgagggtggc acccttaggg atctcggggc cggggaggat
    13561 gggatcgaac ctctcggcgt agaggtcgtg gaaggagacg ccgtggccat tttgggtcag
    13621 cgcctccacc gccgcctccg ctatggcgaa gttgaagctt ccgggttcgg ggtggccgag
    13681 gatgacggat actctcatgg gatcatcgaa aaataaatgg gctttccatc tctttcatcc
    13741 cgatcgaggg ggtggaagag aaccttgggc cctcgggcgg cccgaaaggc ggcttccagg
    13801 ccccccctat cggccagaag ggcccttact tcttctcctt cttctcggga gccttcgccg
    13861 tctcgtaggc gaggatcgag gccttggcgt ggacccggac gaaggggctc tcgtcctcct
    13921 ccgcggccct cttcaggatc accagggact tctcgtccat catctctccc aggacccagg
    13981 ccgcgccgcc ccggatctcc tcgttggcct ctctgtcggc gaggagctcc gccatcctat
    14041 cctggacgag ctctctgccc aggcccgcga gccccaccat ggcgttggcc ttgacgacgt
    14101 cctcggggtc cttgagggcg gagacgaggg gctccacgac ctccggcccc cccttcttgg
    14161 cgagggcgtt tacggcgttc gccctcagga gcatgttccc ctccttggat acgacctcgg
    14221 cgagcttctc gaccggccag gaggcgtcgg ggaggttctc cgggacctgg gggcccgcca
    14281 gggcatcgat cgcctccgtc tcgccgagag ggacggcctc aggcttctcc ttcttcgcct
    14341 tctctgctgc caccttcttc tctttcttcg cttctgccat gggcttttga atgaacccta
    14401 ttctacttca atttttccaa gttcgaaggc cttcctgttg atctcaatat acttcgcggg
    14461 tacggtctcc gtcagcgcct ccaggagggt cgcctcctcc atgggaaggt gcctcgccag
    14521 ggcccccagc atcaccacgt tcgtcgcctg ggggcttccc gcctcagccg ccagggctgt
    14581 ggcgtcgatg gccctgaccc tcgaggcgac cctccggagg ggtgctagga tctcctcgac
    14641 ggcggggtag ctcgcctccc cggtggtgac ggtgacgggg aggacgggcc tcgtattgac
    14701 cagggccacc ccctcagggg agaggtagtg ggcatacctc agggcttctg ccggctccat
    14761 ggccaggagg acgtcggcgc cccccgggga tatcatggcg ctgtagcggc agccgaccct
    14821 gatgtggttt atgacgctcc ctccccgctg ggccataccg tgggtctcga ccccccggac
    14881 cgagagcccc tccctcaggg cggccctgcc gatgatctcg gatatcagga tcaccccctg
    14941 gccgccgacc ccgacgacga cgacgtcgca ctccgatgtc ctcatctcac cacctccatc
    15001 gccccggcgg ggcatatcgc ggcgcagacc ccgcagccgg tgcagagggc gttgatcctc
    15061 gcgacctttc cgtcgaactc gatggcgggg cagccgaacc tgacgcagac ctggcagccg
    15121 ccgcacttct cggtgtcgac ggcgagccga ggccgccgga cccccgccct gatggcgctg
    15181 atgacgcagg gcctccttgc caccaccacc gagaggccgg gatggtccct cgccctctcg
    15241 aaggtcgaga tggtggcctc aaggtcgtag gggtccgcgg tctcgacgaa gccggcgccg
    15301 agagagcggc agatctcctc cagggagagg gggggcgagg ggtcgccggc ggccgtagcc
    15361 cccgagccgg gatggggctg gtggccggtc atggcggtga tggagttgtc gagaatcgcg
    15421 accgtcatcc gagccccgtt gtaggcggcg ttgaggagcc ctggaagccc cgagtggagg
    15481 aaggtggagt cgccgaggac ggcgcatatc ggcctctcct cgcccccgaa cctgatcccc
    15541 gcgccgacgg tgatgctcgc ccccatgcag aggcaggtgt cgacggtccc catcccgacc
    15601 cccatggtgt agcagccgat gtcgtttgga aagattgcat ccttaccgaa ggccttgatc
    15661 atggcgtggt aggcggcccg gtggccgcat cctggacata gagccggggg cctggggggg
    15721 aggatcgaga gggcctcggg ggcgagggcc ggcgcctccc tccgttggag gcctaccatc
    15781 ccggcgatgg cgtttccgac ggtgaggggg ttgagctcgc cgacgctcgg aacgtccccc
    15841 gacctcttcc ccaggatctg gacgtcgggg ttcctctcct tggccaccat ctcgacgaac
    15901 tcctcgacta ccgggtccat ctcctcgacg accaggaccc ggtcgacctt ctccaggaag
    15961 tctccgacga ccttcctcgg gagggggtag gtccctatcc tcaggaggga gaggtccgcc
    16021 tccattccag cgatcgcctc ctcggcgtat agccccgcga tccccgaggc tatgactccg
    16081 acctcgcttc tcatctgtgc cctgttccac gcctccccgg cgagggcagc ctcgatcccc
    16141 ggctgcttgt cgaggagctc gacgtgcagc ggccgcgcgt gggcggggag ggcgaccctc
    16201 ctcactgggt ccttatcgaa gctcagctcg gccctcctct cccccggtgt gcccacctcc
    16261 acgtcggccc tggcgtggga gaccctcgtc gtcggcctca gcatcacggg gaccccgaac
    16321 tcctccgaga ggtcgaaggc ccggagggtc atatccctcg cctcctgggg gtcggcgggg
    16381 tcgaggcagg ggacccgggc gaactgggcg tatcggcgag agtcctgctc gttctgggag
    16441 gagtggcagt aggggtcgtc gg
//
LOCUS       DASM01000171            2973 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_121349, whole genome shotgun sequence.
ACCESSION   DASM01000171 DASM01000000
VERSION     DASM01000171.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_121349"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          588 a          974 c          854 g          557 t
ORIGIN      
        1 ctttgatgat gctgattgag atgagttaaa cacataccat caatcaaatt gcatttatct
       61 gagacgctga attaggagat tcttcggtag acttaaattt ctccagctta gccagatgca
      121 ctgacgccaa taaccgttct tcggacgatc cgatcgacga acgaggccag cccttaccgg
      181 gaggcgcgga ccggggcgtg atgcccccct cgaaggcggg aacaacccgc cccccctcct
      241 tcgccgccgg gaagcccgac tacgccgccg ctcgactcga tccccataac agaaagagag
      301 atatcgacgt aatcgccact ttgagtaagc gatgataccc ttcgagtacc tcctcctggg
      361 agtggcggtg ctgcttcttc tgagcgtcgt ctccagcaag gcgtccaccc agctgggggt
      421 gccggccctc ctcctcttcc tggtgatagg gatgctcgcc ggagccgacg gccccggagg
      481 cgtcgacttc gacaacccct ggctcgccca agccctgggg acggtggccc ttatcttcat
      541 cctcttctcg ggagggctgg acacccggtg gagcgaggtg aatatgggac cgatccggtc
      601 cgggttgatc cgatcgatga ccgaggccgg gccttcccga gacgcgccgc ccgaggcgtg
      661 gggctccccc tcgaaggcaa gggtgcgcct gccgtccccc caccttcgcc gccgtgaagc
      721 ccgcaggaat tatattgaac tgatcgagcc agcatcagag cctcacaaag tcaatctcca
      781 tcccaaaaga tgaatggaat tgatataaac agttggaagt taaacaaaaa aagaactcgt
      841 ttctacgctt cttgcgaaaa acgcccggag gaaggcgccg aggcggaggg gcgggcggac
      901 ctgcctcccg acccctggcg ccgcccaaag atcgttcttc actccaccac ccggcggagg
      961 gtgtcgatga agaccccacc cccttcgccg tcctggaggt agaggagctc gacctcatcc
     1021 tcggagatga tcgtccccac atcgtacccg gagccgaact cggcgatgta gaaggtcgta
     1081 ttatccaggg cgatcacccc ggcaaactcc tctgccatct cgaccccatc ctccgtgtag
     1141 gtcaccaccc cgttgaagat ccgcccccgc tgctcggtga cgttcatggt gaggatgtcg
     1201 gaatcctcgt cgctgacgta ccctccttcg tccccatacc cggtcccgac tccggaccag
     1261 agccccacca ggttcgggat ctccgaagcc gtcgccgacg ccgagaccga agccgtcgcc
     1321 atcgccgcca gggcgatggc caatattcca aatagcctcc acttcatggc catcaatatt
     1381 ctcccgtctc ccagatgaac ttttccacat cggtggcgat gaacctggcc tttctcggag
     1441 agaagacgct gttttggcga tcgaccccgc aagatataga aatcaatatt attatccgga
     1501 cgccccggga gcaagcctgt tgaaagaagg cctaaaagag gaggggctat ggtgggagaa
     1561 gcccagaagg aaaggcggac ggaaagccgt ccgcccccgt caccaggctc actccggaac
     1621 gcggcggagg atgtccaggg ataccccgcc catctcgccg tcccggatgt aaaggagctc
     1681 gatctcgtcc tcggagatga ccgtccccac gtcgtacccg gcgatgaact cggctatgta
     1741 gaaggtggta ttatccaggg cgatcacccc ggcgagcccc tccacgacct cggtatcgtt
     1801 gatgtccagg taggagatgg tgccgttgaa caatcgaccc ctctgctcgg tgatggtgag
     1861 ggtgacggtg tagttctctc cttctccgta gtacccctcc agggggtcgt agcccatgtc
     1921 agggccctcc caggtcccga ccaggtcggg gaggtccgcc ccggagacgg ccggggccgc
     1981 gaggaagaag gccaatattc caaagatcca gattttcttc atggtgggct atggggccgg
     2041 atctctcttg aatttttccc tctcgccccg gaggggcaag aaaggaccaa aaccctcttt
     2101 ctggattgcc ggcaaaaaag gagctctgag gccgggaggg ggctgaggga gacgccccct
     2161 cccatcgccc tttcgccggg ctacctcagc ccaaggagac tatgcacaaa tcaggcgagg
     2221 tcgaaacggt cgaggttcat caccttgttc cacgccgcca caaagtcggt gaggaacttc
     2281 tccatggagt cctcatacgc atagacctcc gcgatggcgc gaagctggga gttcgagcca
     2341 aagatcaggt cgacccgggt ggcggtccat tcgagctccc ccgtgacccg atcccgaccc
     2401 tcgaagaccc catcgtcttc cgccgtcgcc ctccagaccg tcctcatgtc caggaggttt
     2461 gcgaagaagt cgttggtgag ggcctcgggc cgcctggtga agacgccgtg ctgggacccg
     2521 ccatagttgg cgcccaagac gcgcatcccg ccgatgagga ccgtcatctc gggagcggtc
     2581 agcgtcaaga gctgggcccg atccaccagc agctcctccg ccgatacggc gtacttggcc
     2641 ttgaggtagt ttcggaagcc gtcggcagcc ggctccagga cggcgaagga cgcctcatcg
     2701 gtcatctcct gggacgcatc cgtccgtccc ggcgtgaagg ggacggtaac attgtgaccg
     2761 gcgttcttcg ccgcctcctc gatggcggcg cacccgccca ggacgatcag gtcggcgagg
     2821 gagaccctct ttccccctga cgccttgccg ttgaactcct ttttgatccc ctcgagggtc
     2881 gagagcacag tctccagcct ggccggctcg ttcgcctccc agtccttctg cggcgcaaga
     2941 cggatcctcg ccccgttcgc cccgccgcgc ttg
//
LOCUS       DASM01000172            3620 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_134139, whole genome shotgun sequence.
ACCESSION   DASM01000172 DASM01000000
VERSION     DASM01000172.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_134139"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          721 a         1103 c         1022 g          772 t
ORIGIN      
        1 gagctctttg tggattacgt cctggagaac tccgatcgag agaccgccct agcggtcctg
       61 gaccagcaca taggcgatta caaaaactac ttcaccttcc agagtatgat caccgagttc
      121 gagcgccagg cccatatcct ctgcgatgga aaggacgaga taagcggggc cgagctgaac
      181 tccctatgga gagacctctg caaggagtac gaggacgaga atataggcta ctacgacgaa
      241 gactctgcgg aatggacgta tgtagaccac atctacctca ccaataacta ctacacattc
      301 agctactcgc tttcaaaagc gattacccta tctctcttca agagctacaa ggagaacccc
      361 caggagttca acgagagata cgtcggctac ctctccgctg ggacgacgat gaccccgccc
      421 gagaagctcg agaagttctt cgggatagag attaacagga ccctcttcga ggacgccatg
      481 gacgtggtgg agatgagggt gaaccagctc caggagctgg aagagggcgg ggtgacgggg
      541 ctgtgagccc tcccccggcc cccttcgcct ctatttctat tttcttctcc accgccgaag
      601 ggaggaggac tccatcaggc tgatcggtcc gagagggcct ccttcagccc tgggacctcc
      661 tcagccccta tggggacggt gatcgtcgaa ttatcgaagt cgtctcctac gggctcaaac
      721 cttccgccga ggtgctcgga gaggaaggcc tcagcgacgg cgaagaacga cattctgttc
      781 tccggccgag cgaagccgtg accctcatcc ggatagagga cgtaggtcac aggaatcccc
      841 ttctcctgca tcgccccgac gatctggtcc gactcgttct ggctgacccg gggatcgttg
      901 gcgccctggc ctatcagaag aggccgcccg atattctcga cgcggttgat gggagagcgc
      961 tcgaagagga gccgtcgtcc ctcttcggtc ctgtggtcgc ccatccgagt cgcgaagagc
     1021 tcgatctgag gctgccagta ggggggtatc gtctcgatca gggaggtgag gtttgagggg
     1081 cccactatgt cgacgccgca ggcaaagacc tccggagtga aggtgagccc caccagggtc
     1141 gcgtaccctc cgtagctccc gcccatgatc gccaccctat cgggatctgc gatcccctct
     1201 cccaccgccc aatcgacggc gtcgatcagg tcgtcgtgca tcctcgctcc ccattccagg
     1261 tttcctctgt tgacgaaatc cttcccaaag cccgtagatc cgcggaagtt gacgctcagg
     1321 acggcgtacc cccggttggc aagccactga tgggcggggt tatagtccca gacgtcccgg
     1381 gcccaggggc cgccgtggac gtacaggacc atggggaggg gctcctccgg ccttccgtcc
     1441 ccatcccggt cggatccgag aggcaacgta tagtagctca ccaggtttag accgtcccgg
     1501 gagacgatga cggtcgggat catcatcttg gctaaggcca gatcttccag atccttccga
     1561 tttgtgaaga ggaactccgc ctctttcttc tcgctatcgt accggtaata tctgaagggg
     1621 ccatcgtcca ccacgtagac gacgatccag gttctgtcgt cgagagaccg gctcaccacc
     1681 tcgatgtcgc catcgtcgac ggtttcgagg aaggccaggt cgtcggccac cgatccgtcg
     1741 atcaactgcc agtccttccg ctcgtaggtg aaggcagccg cctggacgtt cttctcagtg
     1801 gggtggacca tccagtcgct gaaatccgac ctcggatctt cggcgatcag ggtctttacc
     1861 cccgtcgctg gattcatggc gaagagcgcc gccgtattcc ggccgcggct gtcgacgaag
     1921 tacaagatct ctccggtctt gttgaagccg atcgatcccg tcgtaatcac gtcctccatc
     1981 gggatctcca agaagacctc ccatccgcct tctcccgtcg gccggaagat ctcggcaccg
     2041 ccttcgcagg tcatattcgt cgcaaacctg acggcataat cgtcatcgat gtcgaaggat
     2101 gaaaaatatc ggttctcctg gatcagggtc atcttcccgg tctgtatgtt caggcgatag
     2161 aggtcgtggt actcgggatc gcggtcgttg aggccgacga tgatctcctc agggaagttg
     2221 cggctgacct ttacaatcct ggcctggaca tcctcgaagg gggtgaggtc ttttatctcg
     2281 ccgctcctca ggttgacgct gtagatccgc cagttctcat ccccgtcctt gtcctggata
     2341 tacaggatgt gttcgttggt gtgggcccat gagtaggagc ggatgccccg ataggtgtcg
     2401 ttggtgacgg gctctgcagc ttccgggtcc tctataggtc ccacccagac gttcaggacc
     2461 ccgtctttgg gggcgaggta gctgatcctg gaaccgtcgg ggctcgttcg tacagtgatc
     2521 ttgtcaggat tgccgaagag cagttctcgc gatatgagag aatctgcatc ttccgcgggc
     2581 atctctccat ccgtcgagcc gatcccctgg gcccatccca ctatcgagag gagggcaagg
     2641 accgctactc caaataaagc tcgtttcatt agtatccctc taattgagcg atctgcgaag
     2701 atccgaaaga gaccaccgca ctgatcttat ttaannaatc atgtttaaga ttaatccgat
     2761 cgatatggta tgtgtccagg ctggggagag aggatcgtct cggcccaata gctcccaacc
     2821 cggtatccaa agtataaaac gaagggagag agtatcggcg atcccggcat attggcatct
     2881 gctgctctct tcgatccatt tcatccaggc ctcctcctcc ttcccggcat cgtcctgtat
     2941 atccgggcct cggggtggat cttcccctcc atcctcagcc tctggacctg cgctcccacc
     3001 gaccccatcc tctcggagaa ctggccggct acgagcttct cgagcctctt tctctccggc
     3061 tccttcccct cgccgatccc ggggcaggtg gggttgtagc ctatctcgag gacgatcgct
     3121 cctccggggc cggacttttc gaagacgacg aagggaaaga gacggcaggc cgtcggccgt
     3181 acggggtaga tggtgcatcc cctaccttcc tggtagaaga cgcaggatcc gtccggctcc
     3241 ctcgacctca tcaccgggag gtccaggatc accttcttcc cttcgtcctc gacgatcaca
     3301 gaacccctct tatcctggta gagggtgtcg acgaactctt cggggatgag ccccgtcctc
     3361 ctcccgatgg cgtcgatgtc cctctccgtc aggtagatga ggttgctctt ctccaagaac
     3421 tccgccatcc tctcgggggg gatgaggtcg tcgaactccc ggggcctccg gtgatggcag
     3481 caggagccgc agcgctgaca cttgaaacgg gcaccgccga aggcggctct gatctccacc
     3541 cggaccggca agaagatccc tcaaaaaaag aaaaaagaga agggggtttc aaaggagctt
     3601 cttgatcggg tggttcgggt
//
LOCUS       DASM01000173            7296 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_90457, whole genome shotgun sequence.
ACCESSION   DASM01000173 DASM01000000
VERSION     DASM01000173.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 7296)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7296)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7296
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_90457"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         1439 a         2263 c         2176 g         1414 t
ORIGIN      
        1 ggttaatgaa acccatattg tttatacaag tgaaatccaa gatgctgtgg tcaaaaacct
       61 gacgcaggcc aaatgcacta acgaacatcc ttgcgatgta gatcaagctc ctgtttgtat
      121 cgccatcaag gacggacagt atgaagtgga tggtgttaag tatgattggg tatcaaccta
      181 ttcttatcgc ggatggtgca gtcttttgaa gaatctaaca gcttgtgatc aaaactcaac
      241 aggatatacc ggggataggt attgggctat ctgcgacgcc gctccatgca cggaaaacca
      301 gaacggatcc aatccggata agcccttgat ctgccagtgt cggataaatg atacaccgtt
      361 tgtgggaatg aatggcagtt gcaccggtga cggtggcggg ataatgtcaa gcatgccgct
      421 atgggcatgg gacttcagca acaacacata cccgtttgct atgcctggct atgaatatgt
      481 acagggcgca tgtgccgcgt gtaaatctga tccattgccg ccgtgatgat ctttcacggg
      541 gctagttggg tcttcggtaa aaatagtgag ggagggtcac tagaccctcg ccctccgtcg
      601 tcgtctctcg tcgtttagga tagccgccgc ctcttcatgg gtcatcccca tggcccgggc
      661 cctctccgga gaggtccgga gggctcgcct ggccatccac agttggtgtt ctctagaggt
      721 attctccatc gtcgtcaccc cagtttgccg aaagccacag gacagaaccg tcgaagcccc
      781 tcgatttgga gatactgaag aagactttag gaacagtcta ttggagatcg ttgccccgcg
      841 aactcaaata tttcagagcg actatttcgt ccgtggctac ataaagcttg cggagatcgc
      901 cgccgaccgt cgcgctgcca ccttccgcag agtcctttcc ttccattgag gagagcccct
      961 gagagccagg cgaacttcat ctcggtgaag gggccggagt cctctccaag tgggtcggcg
     1021 agtcggatcg gacggtgagg gggagcggtg agggggacct tcaggaaagc gagacaggcg
     1081 gcaccgaccg tcctcttctt cgacgagatg gacgccatcg cccccgcccg gggcggcaga
     1141 accagcgact cccacgccat cgagagggtg attttcttgg catttatatg gacgatttat
     1201 cgagcgatct catctcagat attcgatggc cattccgggt tggtacgata tgcctggttt
     1261 ggccagcaat tctacaaacc gacatccctt tgatggatga aagaggttgg cagaggcgct
     1321 gcttctgatg acccatgctg tatcaagacc gcgctctgtt cctgcgacag cgagaacatg
     1381 ctcttgttct ggaatcgccc ccgcatcgac cgccatcaag acacattcca aagagaccaa
     1441 cgtcccggtg gaaatcaact ccaaggtctt tgccagaata tcaagggatg taacctgttg
     1501 aagatggagc ctcatcgaaa aacttggacc aaagaaggga attgttcctt gaatgatggg
     1561 gacattcagg gctgttaatt ttagtctatt ttccggagtg atctgcatgg gcacccggga
     1621 gaggccgcgt tcctcccatt catcgcgcac cttctgaagt tcaggaatat ctgcaaacgg
     1681 accgctctgc atctggtcaa cttcccatcc cattccttgg ggacatgtga cacagatgat
     1741 ggtcgtgtca tggattcttt cagcgatgcg tacagcacat tccccagtaa ttgaggcgac
     1801 gacaagatag cgatattcag aagacaccgt cttcacaatt tcaatcgttt catctgtgtt
     1861 gacgggtccc ggcttttcaa atactctcat aattctgcct cttttaaatg atttagctca
     1921 caagctgcct cccgggcgcg gctagccatg gaaagctcga aagagttctg aaggggcgta
     1981 gatcttctcc atctcgttaa atattcaaga tttcatatca tatatagtgt atgtcgacca
     2041 gaagaaagat gctatggggt ggcatgaata cttttcctcc tttcgccggc aacgccccat
     2101 aagttcccag gactcgaatt taccgaaccg ctcatcatcg ttcttcagcc cttccgtctc
     2161 cttccccccc tggctcttcc ctgaaaccca ttcttatgta acccgaggaa aagatttaac
     2221 cggccaggtt acataagacg cacccttgat ccccaaattc atcctggagg agctcgccga
     2281 ggaggacctc ccccacctcc tggaggtgag ggactacctc gaccggctga taagagatcg
     2341 cgaagagtac cccgttgagg atggctccga agccgaggcc agcaggagga ggcccgcctc
     2401 cgtcacctac cggcaggagt gggtatactg cggcaaggag tgcaagacct gcccccacgg
     2461 gccctactgg tactgctact ggaaggagga cggaaaaacc cggacgaagt acatcggcaa
     2521 ggtcctcaag gaggtggagc tgtgaccgca gctcaccatc cctttctggc cctggcccta
     2581 tccctcgccc tggccctcct ctgcggctgc ctctccgccg acgagccggc cgccgccccg
     2641 gaggggggaa aggtgaaggt cgtcgcctcc acccttcccc tgggcacctt cgcctccatg
     2701 gtgggcgggg acctggtgga ggtcgcggtc ctcgtcccgc cgggggcgag cccccacacc
     2761 ttcgagccga ccccctcgaa gctcgcggat gtggcggagg cggagctcta catcaagaac
     2821 ggggccggcc ttgagatctg gatggagagg atgatccagg cgaataggaa gatgctcgtc
     2881 gtcgacacct cctccggggt ggagctgata gagacggccg gctcctcctg ccacgagaag
     2941 atcctggcgg cggaccccca catctggctc tcgccgaaaa acgccatgat catcgtcgag
     3001 gagatctgcg acggcctggt ggaggtggac cccgagaacg ccgaggctta tcggaggaac
     3061 agggacgatt acctcctgag gctcgaggag ctcgaccgcg agctagagtc cgccttctcc
     3121 ggggcctccc ggagggagtt catcgtcctc caccccgcct ggagctacct cgcccgggat
     3181 tacgccctcg tccaggtggc gatcctggag ggggataagg aacctgggcc gcggtacctc
     3241 gccgagatcg tcgaggtcgc ccgggaggag aacctcacca ccatcttcgt cgacccgaac
     3301 ttcagcccca agtcggggga gatcatcgcc ggggagatcg gcggacgggt cgtacccctc
     3361 gaccccctcg ccgaggatta catcgagaac atgagggccg taagccgaga gataggaaag
     3421 agcctggaag gctgatagct ggagaggaga gaggaatgac cggcgcagat cccgtatcga
     3481 tccagaacct ctgggtccgt ctggacgggc ggaccgtcct ggaggaggtg aacctggagg
     3541 tgaaggaggg ggacttcctc ggactgatcg gcccgaatgg cggggggaag acgaccctcc
     3601 tcagggccat cctggggctg gtgaagccct cccggggaag ggttgccgtc ttcgggatga
     3661 gccccgaggc ggcccggtgc cgggtcggct acctccccca gaagagcctc ttcgaccaga
     3721 ggtttccgat ccgggccctg gaggtggtac tgatgggccg ccgggggagg atgggcctct
     3781 tagaccgcta ctcctcagag gatcgcgagg cggccctctc tgccctcaag gaggtgggga
     3841 tgctggatct gaaggaccgg gagatagggg ccctctccgg gggtcagcag cagcgggtct
     3901 ttgtggcgag ggccctcgtc tcggagcccg agctcctcct cctggacgag cccgccaccg
     3961 ggatcgacag cgccaggcgg cgagagttct acgaactcct cggcgacctc aacgagaaga
     4021 tgacgatcat catggtctcc cacgacatca gtgcagtatc cacctacgtc aagaagatcg
     4081 cctgcgtcag ccggaggcta ttctaccaca gctcaaagga gctcttcgcc gaggagatcg
     4141 aggaggcgta ccagtgcccc gtggagatga tagcccacgg catcccccac cgggtcctga
     4201 gggagcactg accgatatga tcgaggcctt cgcctacgag ttcatgcaga gggccctcgc
     4261 cgcaggcttc atcgccgccg tcctctgcgg cgtcatcggg gtatacgtca tcctcaacag
     4321 gatcgtcttc atcggggggg ggatcgccca caccgccttc ggcgggatcg ggctcggcta
     4381 cctcctgggg accgaccccc tcctcttcgc cgtcctcttc tccctggcgg ccgccctggg
     4441 gatcggggcg atcagcgacc ggccgagggt ctcggaggat acggcgatcg gcgtcttctg
     4501 ggcggcgggg atggccctgg ggatcgtctt tatcagcctg agccatggat acgcccccga
     4561 cctcttcgac tacctcttcg ggaacatcct ggcagtatcc tggggcgacg ttctcctcat
     4621 atcggtcctc accctcctga tcctcggcct ggtggccctc ctctacaagg acctcctgat
     4681 cctctccttc gaccccggct acggcgaggc ggtgggcctc ccgacgaggg ggctgcacct
     4741 cctcctcctc tccatggtgg ccctctccgt cgtcgtcctg atcaagatcg tggggataat
     4801 cctggtgata gccctcctca ccatccccgg agccatagga aggcagcact cctccagcct
     4861 caaagggatc atgaccctcg ccatcctcct cggcggcgcc ttcgtcctgg gagggctcgt
     4921 cgtctcctac cagctgaaca tcccctcggg ggcgacgatc atcctcgccg ccgcgggtgc
     4981 cttcctcgcc agcaccctcc tcgcccgatg aggggacgaa ttccggcctc ggaggggact
     5041 tgcacaacaa acttatacgc cggggcgcta aaggcatgat cgggatgcag aaccatcaga
     5101 tccaatccat actcctcctc ctcctcctgc tggcccccgg ggcggcgagc ctggcagagg
     5161 gtggtggggt ccagttcgcc ggcgaccact acaaggtcca ggggggtccg aagatcgacg
     5221 tctccctcat aaaccccgtc ttctcccccg gtaccggtgg gaccctccgg ctgatcctcg
     5281 ccaacgacgg ggtggtggag gggctcgtcc ccggagccgt ccccccgggc tccgaggagg
     5341 atgcagccca ggagatgctg gaggagtttc ggtgcctcga ggccgcgaac ctccgggccg
     5401 acctccttgc cgcggcgcct gtccgggtcc tctcggggcc ggtccagatc gacagcctcg
     5461 gccctggaga gacctcgccc ctggagttcg agatagagat cgaggagggg ggcgagggtc
     5521 cgatcctcct cagcctccag gtcgagtacg agcaccagac cgacgtctcc gtcagcggcg
     5581 gcggggcctc gcccctctat ctccccacca tcctccagct cgacctgatc ctatcggtgg
     5641 agggcgagcc tccggccttc aagctcatct cgacgaggtc gggcctctcc cccggcaagg
     5701 agggagagat cagcctcgtc ctcgggaacg ccggaaaggc cacggccaga aactgcaccg
     5761 cccggctggt agcagccccg cccttcatcc ccgtctcgaa tatctctcgc ctcggagata
     5821 tccccccggg gaggacggcc gtcgcccgat tcgaggtggc ggtggagggc tccgccccgg
     5881 ccggtgagta cagggtcggc tgcgagatta tccacgatgg tgggggctca accctgtcca
     5941 tccccctctc cgtcgtaagc cccgaggaga gccgcctcct cagtggcaga ttaatcgggg
     6001 ccctcctcct cgccgcggcc ctggcagccc tctggcacct ccgatcgagg atgaaggggc
     6061 tttcgcggag ccgccggacc ctccagccgc gacgataggc ctggagcgac tttcggggag
     6121 gtcaaccttc cgcccaccaa cactcatatc ttcaacctcc gtctacaccg tcacctatgg
     6181 cagcgctccg ggccgagaag atctccaaga gctttcgggg gctcttcaag aagaaagagg
     6241 tcctccacca ggtatccctc gccatcgagg agggagaggt cttcgggatc ctcggcccca
     6301 acggcgcagg aaagacgacc ctcatgtcga tcttctccac cctcatccgg cccgatgagg
     6361 ggaggctgga ggttctgggt ttaaacgccc tccgggatac ccacgccatc agggagagga
     6421 taaacatcag cagcggaaac cccaactttc cctggagcct cacagtgagg gagaacctta
     6481 accattcggc gatgctctac ggcctctacg gccgggacct cgcccggtcg gtggaagggg
     6541 cgatctcctc cttcgatctg gaggctcacg tcgactcgaa gttcgagacc ctttcgacgg
     6601 ggctgaagca gagggtatcc ctggcgaagt cgatcctcaa tcgccccgag atcctcttcc
     6661 tcgacgagcc gannagccga cctcgggcct cgacccggat atggcgatga agacgagggc
     6721 cctcatccac agcatctacc gggaggagga gatgaccgtc gtcctcacga cccattacat
     6781 gcccgaggcg gaggagctct gcgatcggat agccttcctc cgggagggga ggctgatcgc
     6841 cctcggaacc cccggggagc tgaagaaggc ccttaagatc ggggaccgga tcttcatcga
     6901 gtacgaggga gagatcgatc cgatcagcct cagaataata cccggggtga tggacgtcag
     6961 gccctcggcc ggcagggtgg aggtcgtctt cgaccggagc ggtggtgccc tcgggaggat
     7021 aataagggcc ttcgataggg cggagatcct ggagatgggc ttagaaaagc cggacctgga
     7081 ggacgtcttc cttgagctgg nngagctggc gaggtgagct gaacaagctc ctatcggcga
     7141 cgtacaagaa ctggatcata tcgaagagga acgtcttcac cgtcttcgag ctgatcttct
     7201 ggcccttcat cagcctcctc tcgataggcc tcctcacccg gttcctggag gtggccgacg
     7261 acatggtcgc cttcgtcctg gtgggggcga tcgccc
//
LOCUS       DASM01000174            3018 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_263648, whole genome shotgun sequence.
ACCESSION   DASM01000174 DASM01000000
VERSION     DASM01000174.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_263648"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          706 a          864 c          917 g          531 t
ORIGIN      
        1 acggtccgct tggtcaggtt gatcagtttc ttcatcgccg cgttcttgag tgggctgacg
       61 gcgctcaggt caacgtcact ctttaaaaga acgccacggt cactatagag gtctattttg
      121 tcagacacgt tctttttcct cctttacctt ttagcaggag accgaacaaa ccagggatgc
      181 cggagcgaac ggtatgatta atttctagtg ctatcatacc cgacaatgat cctctttact
      241 gcagtctccc gccgaagaga tgatagtcat aacgctactt aaagtttttg tgcgatacat
      301 ttaataacaa tacatttatt cgaatgtgta aaaatattgc ccatagtgtg aaaaaaagat
      361 aaaaaaataa tcttaccggc gcatggcata aaatggtgat gatagacctg gactatgtgg
      421 ctcgtaatcg gatttcaggc cacctttcgg cgagataata tcagcggatt gatccatcgt
      481 atcctatgga gagatcgaag atcaaggcaa atattttgat gtgaatacag tacggatgtg
      541 attgtgttac aatggcctga cagtctccga ccgtttgcac ataatatgtg gtatctgtaa
      601 acagacgatc cggggggagc cgcgatggca ggaatgtgat gaaaatgaag ggcgcgttta
      661 aggtgaaaac gccatctagc gacttggatt cctggatcgc ttctgatgag gcccttcgag
      721 ccttgcgata attacttata tcatctccaa aactggcggg gatcggatgc atctcatcat
      781 cgccgaaaag cacaatgtcg ccaagaggat cgcccaaatt ctcgccgggt ccaagcccaa
      841 ggtcaaacgg gttcacggga tcgaagcatt ttacttcgac gataaggtcg tcatgggcct
      901 ctccggccac atagtcgggg tcgactaccc gcccggctac aacaactggc agaaggtgga
      961 ctaccgagac ctgatccacg ctgaggtggt gaccaggccg acccagggca agattgtatc
     1021 cgccctccac gacctgggaa aggatgcaga ccggatcacc atcgccaccg actacgaccg
     1081 ggagggggag ctgatcgggg tcgaggcctt gaggatagcc gaggatgcga acccaacgat
     1141 caaagccgac agggtgaggt tcagcgccat caccaagggg gagatccagg acgccttcga
     1201 atcccccggg gaggtggact tcgacctcgc ctcctccggg gaggcgaggc aggtcgtcga
     1261 cctgatctgg ggcgccgccc tcacccggtt catatccatc acctccggga ggctggggaa
     1321 gggcttcctc tcggtgggga gggtccagtc cccgaccctg gccctcatcg tcgaccggga
     1381 gaaggagatc gaggccttca agccccagcc ctactgggag atcagcgcct gcctcgagaa
     1441 ggacctccgg gtgaagcatg agaaggggag gatctgggac ggcgagctgg cgaggtcgat
     1501 cttcgaggag ctcggggagg tcgccaccgt agtctccatc aaggagaaga cgcggaccga
     1561 caggcccccc gcccccttcg ataccaccgc cttcatcagc gccgcgagcg gcatcggctt
     1621 ctcagccgcc aacgccatga ggatcgccga gtggctctac accagcgggt tcatcagcta
     1681 tcccaggacc gagaacaccg tctacccgaa gaccctggac ctcagggccc tggtatcgat
     1741 cttcaagaag gggcccttcc gggaggacgc ggagcgggtc ctgtcggggc cgatggtacc
     1801 gacccggggc aagaggttct cgacggacca cccccccatc tacccgacgg ctccggccac
     1861 caaagcggat atgaagcccg accagtggaa gatctacgag ctcgtcgtca gaagatttct
     1921 ggcgaccctc tccggcccct cccgctggat cgtcatgaac gtccgggtgg agatcgaggg
     1981 ccagcccttc agggcgacgg gtgcccggca gattgaggcc gggtggcggg cgtgctaccc
     2041 ctacagcaag gccgccgagg agatcctccc ccagctctcc gagggggaga agctccgggt
     2101 cctggggaaa gagctggagg agaaggagac ccagccgccc tcgaggtacg gccaggggag
     2161 gctcgtcaag ctgatggacg agctggggct ggggacgaag tcgacgaggc acgagatcat
     2221 aagcaagctc tacgcccggg cctacgtcca gggaaacccc atgaagccga cgaacacagc
     2281 ctacgccgtc gtcgaggccc tgcagcagca ttcgcccaac atcaccaagc cggatatgac
     2341 ccggaccctg gaggaggaca tgacgaagat cgccaacggc cacgtcggag aggaggcggt
     2401 gatcgaggag tcgaggtcga tgctcgacgc cgtcttcgac gacctcctcc gccaccagga
     2461 ggagataggg acgaccctcc gcgaggggct ccggaccgac aagatcgtgg gcctctgtca
     2521 aaactgcggc cacgagctga tcatccggag gggaaggcgc ggcagcaggt tcataggatg
     2581 tgccggctac cccgactgca ccttcaccct ccccctgccg aagttcggga tgatcgtcgt
     2641 caccgacaag gcctgcgagg agcacgggat gcagcagata aggatcatca acaaggggaa
     2701 acgcccctgg gacctcggct gccccaagtg caacttcgac cagtggcagg cgaagaaggc
     2761 cgaagaggcg agagagaagg ccgaggtcga cctcctcgcc aagatccccg ggatcggccc
     2821 caagaccctg gagaagctcg aagccgccgg ggtgaagacc gccctcgacc tggccggggc
     2881 cgaggctgag gagctggcgg agaagaccgg ccttactaaa aagaagatat cttcgtggca
     2941 gctcctcgcc aggacgatgg taaacgaggc acaaagctgc tgatttaatg aatatgggcc
     3001 tggccggatt tgaaccgg
//
LOCUS       DASM01000175            3911 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_60823, whole genome shotgun sequence.
ACCESSION   DASM01000175 DASM01000000
VERSION     DASM01000175.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_60823"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          628 a         1325 c         1184 g          774 t
ORIGIN      
        1 gggcgaggat ctccatcagg tccgaggtcc ccccagctcc ggtgatcgcc ctcgagctgg
       61 tcttggggat gagaagcccc gccgccgcca cgatcggcac caccaggagg gagaccttgt
      121 ttccggggac cccccctatg ctgtgcttgt cgacgacggg gtgggtgtcg aagtggatcc
      181 gctcgccggt atcgatcatc gccctcgtca gccactccgt ctcctgggag tcgaagtttc
      241 ttatgtagag ggtggtgagg taggcggaga tctcgacgtc gctgaggttg ttgtcgacga
      301 tgtcctggac gatcgttctg atctggtcct cggtgagcct ctcatggtcc atgatcctct
      361 tgatgtagct gatggaggcg ggcttcggcg ccggggcgac gtccacctcc ctcgcctccc
      421 cgatcctccg gtgggcctcg gtgaagatcc cgaccttccc cggctccacc atctgggtgg
      481 tgacgtccag gatcgcggtg atcgacgccc ctttcgcctt aacccggacc cgatccccgg
      541 cgttcatccc catatctctg gagtcggcca ggttgagcat caccttgtat tgtcctatct
      601 ctatgtcgaa ggggttgacc tcgaataaca tgttgaacct ctctcgtgct tctggaagcg
      661 tctgatgctc ccatatctgt aactcaaccc ttaactaacc ctccgtccgg tctcgggggg
      721 gccagctctg ggagagcgcc ttcaggcaat cgatcatctc ggagaccttc cccctcgcct
      781 cgtcgtggtc ggtggtgcag acgatccgaa actcctcttt agattcgagg atcatcacgt
      841 ccgccccgaa gatcggccag gagaggtcca cggcggcggc ggagatctcc cgccaggcgg
      901 agggcctctc ccggacgaac tcccccgtcg tcgccagggc ggcctcgacc cctgccctca
      961 gccggtcgga gaggtcgatc ctctcccaga agtcgtactc gggccggaga tccgggtaga
     1021 gctctcttat cgccgtctct aggtagaggt tgtaggcgac ggtcctggtg gggtcgaagg
     1081 agaggacgcc gcacctcgcg atccgatcga ggttctcccg ggccgtcgga aagtaggagg
     1141 ggaagatctc cgggtcggtc ctctggacta gcctctctat ccccagatcg acgaggaggt
     1201 tcgtcatctc caggacgatc ttcgagcaga agtcgcaaga ggagcctatg tgacgcttca
     1261 tcaccctctc ctcgaagagg acgtggtaga actcgtggag gagggggtag ggctccgcct
     1321 ccgagaggta tatccggtac ccgtccttct cgtggaccgt ccttcccagg acgaagggct
     1381 cctcggtgat ctcgaccgcc acctccacct cgagcctcca ggacctcttc agcccttcca
     1441 ctatcttttt gaggccctct ccagcagggt cagagaccgg atccactctc ctctcgcctc
     1501 cctggtcgtc cccaccacca gcctcctccc ggcgaccgtc tcttcgacga cgccgcaggc
     1561 ctcgatcctc tcccccggga gggcctggcc tgcgtaggtg tgggtgtagc agaggatctc
     1621 ggggatctcc gggtggtcga tctcgaagac ggcggggttg tcgaaggcga actccgcacc
     1681 gaggacggtc ccctcgatcc tccgccggcc cgccttcttc cccggcggga aggggggcgc
     1741 gatctggtcc cagtcccggg tgaagaggag gtcgaagtag gtcccgtcga cgagaccccg
     1801 gttccccttc ctcttctcgt ggaggacgaa ctcgtcgcgg cccgtctcgg ggacccgctt
     1861 ctggtatatc ctctcccagg tcgcctcgtc cagctccttt atcctgccgc cgagccgctt
     1921 cgcctccgct atcacctccc tcgccctcca ccagtccctg ccgtagacga ggaagtctat
     1981 gtcggagccg ggcccctcca gccccaccag gatcgatccc gtgatcccca tcttctcccg
     2041 ggggacgccg cccttatcga ggaccgcgac gatctcggct atccggtcgt ccctctccgc
     2101 cgcccgtgca agctcctcct ccggccggaa gatccggagg acgtcctcct cggggacgac
     2161 gtggacgtcc cggacgtaat cgggcctcgc ccgccggagg aactcgaagg ccacctcgaa
     2221 gtcgagcttc cgatatcgaa ctcccccgct ggccctctct cccttcgggt ccgggacgta
     2281 ccggaggatc gacctgatgc cctcagggtg gtggtagtcg acgacggcga agatccagcc
     2341 gtcgacggtc gcaaagaagt ctctgatcct cgccctgttc ataagagccc ctccagcctc
     2401 tccatccccg cccccctccg gatcaccttt ctctcggcga ggtcgacgag ggtcgagggg
     2461 aggccgtacc tcgacctccc accgtccagg acgaggtcga cattcttccg gatctcgccg
     2521 tccagctccg cgaggctggc ggggggcggt aggcccgaga ggttagcgct ggtggaggtt
     2581 atcggccccg agagggcgat cagctccagg gccatcggat ggtccggaaa cctgatcccc
     2641 accagctccg atccggcggt gacgaggtcg gggagcttcg gccccctctc gacgaggaag
     2701 gtgacggggc cggggaggac cctccggagg agggggaggt caccatcgtc gatccgggct
     2761 atctccccca tcatctcgaa gctggcgacg gcgacggaga ggggcatccc ccgggggcgc
     2821 cccttgatct cgaagacccg ctccacggct cgggggatga agaccgaggc tccgatcccg
     2881 tagaccgtct ccgtcgggta gacgacggtc ccgcccctcg tcagcacctc cgccgccctg
     2941 gatatctccc tcgtcgtcat ctcttcttga tggaggcgat gtcgccgagg gtgaggccgc
     3001 ggcccccctt ccccgatttc catccatcgt cgctcgattt gtccgtcagc ttcacgccga
     3061 gctcccgctc gagcttgacc ctcaccgagt cctcggggac tatctcgtgc ctctcgatct
     3121 tcctgatgag ggaggccttc tccttgatct tcagccccag ctcctcgggg gtgaggccca
     3181 tcccctcccg ggcctccttc acgatctggc cgtattcggg gacgatctcc ggcatctcct
     3241 tgaactcgtc gcgacggggc ctgggcttgg ggggggctct gaaggcccgc tcctggggga
     3301 tcaccttcct cgggacgggg gagaacttgt ctgcgacctc accaaaccgg gcgcagctgt
     3361 tgcagacctg cagttcagat ccgtcgatga ccaccttctt tggcttttcg gagacctctg
     3421 caccgcatat ctcacactgc atattctctc aactcggaaa agtttaatag cagtcaacgt
     3481 taaatacgct ttggagatcc catggacgaa tcgcgagtcg gagctgattt ttcgcgctac
     3541 ctgatggaca ggatgcgaca gctcgaagag cggaacctcg ctcttcgaga gcagaaggat
     3601 cgggtggagg gcgaaaagcg gatcatggag aaccagaagc tcaagttcga gcgcgaggtg
     3661 agaaagctcc gcagcgagct ggagcggctc cggactggcc ccctcatcgt cggcaccatc
     3721 caggacgtcc tcgacgacaa ccgggtggtg gtcaagagca gcaccgggcc gcgattcgtg
     3781 gtcaacgtat cccagttcat cgagggggag ctccggccgg ggacgagggt ggccctgaac
     3841 cagcagtcct tctccgtcat gttcgtcctg ccttcgtccc gggaccccgc cgtcttcggg
     3901 atggagatcg a
//
LOCUS       DASM01000176            4199 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_174351, whole genome shotgun sequence.
ACCESSION   DASM01000176 DASM01000000
VERSION     DASM01000176.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_174351"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          799 a         1309 c         1390 g          701 t
ORIGIN      
        1 ccacccacaa gaccctccgg ggcccgaggg gcggcgtggt catgtgcagg caggagatcg
       61 cccccgccat cgacagggcg gtcttccccg gggtccaagg cggccccctg atgcacgtca
      121 tcgccgctaa ggccgtcgcc ttcaaggagg ccctggcccc cgagttcaag gtctaccagc
      181 agcagatcgt gaggaacgcc gccgtcatcg ccaggaccct ccttgagagg gacttcgagc
      241 tggtcagcgg cgggaccgac aaccacctga tgctggtcaa gctcctgaag atgccctact
      301 ccggaaaaga tgccgaagcg gccctggagc gggcggggat caccctcaac aagaacacca
      361 tccccttcga tccgacctcg cccttcatca ccagcggtat caggatcggg acccccgccg
      421 tcaccacccg ggggatgaag gagccggaga tggtccagat cgctgagatg atcgcctccg
      481 tcctccggaa cataggcgac gagaaggtca tagccagggt gaaagacgag gtggctgagc
      541 tttgtctgaa gttcccccta tatccggaac tgggatgatc atcgacggca aggccctcgc
      601 ctccgaggtg gaagcagaga cgaaagtgag ggtcgatgaa ctctcagccc tgggcggaag
      661 gccgggactt gcgacgatca tcgtcggcga taaccccgcc tccgcgatgt acgtcaggat
      721 caagcactcc gcctgcgatc gggtcgggat aaggtccgag aatgtgaacc tcccggaggg
      781 cgcggggatc gatgagatcc tggagacgat ccggggcctc aacgagaggg aggacgtcag
      841 cggcatcctc ctccagctcc ccctaccccg ggagatcgac ccggcgcggg cgatggcggc
      901 catcgacccc gcaaaggacg tcgacggctt ccaccccatg aacatgggct ccctcctcgc
      961 cggggccgag gggttagttc catgtacgcc gaaggggatc atctgggccc tggagaggct
     1021 tggggttgac ttagagggga aggaggtggt gatcgtcggc cacagcaacg tcgtcggaaa
     1081 gcccctggcg gcgatgatgc tcaacaggaa cgccaccgtc tccgtctgcc acgtcttcac
     1141 caaagacctc gcatcccaca cccggaatgc cgaggtcctg gtggtggcgg cgggggtccc
     1201 gggcctcatc cggggggata tggtccggcc gggggcttac gtcttcgatg tgggcataaa
     1261 cagggtcggt ggacggaccg tcggggacgt ccagttcgac gaggtgatcg gggtggctgc
     1321 ggccgtcacc cccgtccccg ggggggtcgg ccccctcacc gtctcgattt tgatgagcca
     1381 gacggcgaag gcggccaaga tgcagatgaa gatgaaggaa gggtgaaaac ccggatcgaa
     1441 gggtttcgaa ggaagggcga gctggatgtc tctggtggtg ggctttgcgg ggaagaagag
     1501 ggcgatcgta tccggggaca ggaggagcat cgccttcttc ggaagagccg aggagctgga
     1561 ggaggagctt tacggcggcg agatcaggag ccgcgaggat ctccagagga gatgcgaaga
     1621 gctcggctca aaggtcgcca tcgccgacgg ccgggagaag gtctgggata gaggaggcgt
     1681 cctggtgggg gaggtgacgg agatctccgc cgagagagag aggcggagga gggtttacct
     1741 cgtccccgga gggtacctcc tggtggaggt cgacggcaag agagccgaga tatcgaagag
     1801 gggggcgtcg acgctgatca tcctcggaaa caggttcacc cgagatttcg cattcaagcg
     1861 gctcggatct gggaggccgc cggacgaggc gtctatcaga gctttattcg aggaggcggg
     1921 gagggccacc gcctccgtca gccccgatta taccgtcctc acctccagct cgatccatcg
     1981 agaccctgaa gcggccctcc tccaggccct gagggaggac tgcgaggagg gaggatggga
     2041 gctctccggt cttgcgtgat cctcgccggg ggcgccggaa ggaggatcgg ctcggagaag
     2101 gcgttcctcg agcctttcgg ccgaacgatg atcgaggcga cggtggaggt ggtcgggcag
     2161 gtggcggccg atctggtggt ggtggccagg gaccttgatc agctctcgag gctccggggg
     2221 ctcgtccccg gggcgaggct cgcctccgac cccgtccagg gcttcggccc cgtggcgggg
     2281 ctcgccgccg ggatggaggc ggcatcaggc gagtacgccc tcgccgtcgg ctgcgacctc
     2341 ccctttctga acccccgggt tctggagctc ttattcgacc tcgccgaggg gcacgacgcc
     2401 gcggtccctg aggggaagag cggcttcctg gagccgctcc acgccgtcta cagggccggc
     2461 cccatggcgc gggcctgccg ggaggctctg gccgcggggg agcggaggat ccgggccccc
     2521 ctggaggggc tagcggtccg gaccgtcccc ctgaatctcc tccgccccct cgaccccgag
     2581 ctcttgagcc tcttcaacct caacaccgac gaggacctca tcgaggcccg ccggatcgag
     2641 gaggtcagag gtggcgccgg aggacgaggt atacgacgta cacgacaaga gcgaagatcg
     2701 ctatcccggc tagaaagcct atgacgttcc agaggaggac ggcgactacg aaggcgagga
     2761 cgaggaggag ggcgttcttt gcgagggagc cgctcccccc cgccccggcc gaaatctgac
     2821 gaggcatggc tccagttatc acccttcagt tgataacgat ttccgttacg attttcccac
     2881 cgatctcctc agggaccaga atctcctatt tcgcgattcc gccgtcaggt acagctctgg
     2941 aggaggcggt agaccaggta gacgacggcg gcgaagagga ggagccggag gatgaccccc
     3001 aggaagttcc agaggagggc gacgatcacc accgccagga ccaggaggat gatcttctgc
     3061 tcgcggctcc ggatcctgcc tgatctgccc gccattctgc atctcatggc tgggaggtgg
     3121 agcctgcctt ctgataaccc tttccgtctg ggacgggcct gaattagcct cgagacctca
     3181 atccgagata gaggatgtag acgatgacga aggcgaggag gaacttggag aggaaccaga
     3241 acgcatccca gaagatcagg gcgaagagga gggcgaggaa gaggaggccc accgtcttct
     3301 ccttcctcct ctcctctccc aactctgtgg gggccgctgg ccgatccgta tcgagatccg
     3361 cgctgcgatc cgcctccctg gccctcctcc tccccttccc cagctccttc ggcctcatgc
     3421 cctcccgttc tggggcggcg ggtaataacc ctttcccgcc gtcttccggc gccttctgcc
     3481 gccgcataaa cccgcccttc aaggtcggtg tatcctcatc tcttcatctg gtcacctcat
     3541 ctcatcttct cataatctca tcttctcttc atatggtcat ctcatcatct caccatctcc
     3601 tcccccctca accagaccct ccctcctcga ctccgccacc tcggccgccc tgacttcgag
     3661 gtcggctccg ccgaaaacgg cgccgatggc tcggagccgg caaaatttgg tcttccgccc
     3721 cgaggaacca aaagcgttat acatcccctc tcctctgggg ggttggtgat atctattgga
     3781 gaggcttgag ctggtggtca ggaatgcaga ggaggtcgtg accctcgagg agctgaaggc
     3841 gctgatgggg gcgaaggaga ggccgagggc ttacgtcggg tacgagccga gcgggaaggt
     3901 ccacatgggc cacatgctca ccgcaaacaa gctgatcgac ctccaggccg ccgggtttca
     3961 ggtagtcgtc ctcctcgccg accttcacgc ctacctcaac gagaagggga ccctggaaga
     4021 ggtgagggcg accgccgact acaaccgcga ctgcttcgtc gccctgggcc tcgacccgga
     4081 aaagacggag ttcgtctacg ggaccgacta ccagctcgag cctgagtacg tcaagaacgt
     4141 cctgaagatg gcgcgaaaca ccaccctcaa ccgggcccgc cggtcgatgg acgaggtgg
//
LOCUS       DASM01000177           32150 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_31364, whole genome shotgun sequence.
ACCESSION   DASM01000177 DASM01000000
VERSION     DASM01000177.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 32150)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 32150)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..32150
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_31364"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    31712..31827
                     /estimated_length=116
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         5915 a        10177 c         9829 g         6102 t
ORIGIN      
        1 tcgacctcga tcacctcgcc cttcttgttc ttcaccagga gccagaggtg atcggcgccc
       61 cggccccgga aatcctcggc gtaggcgaag gtgacgtcaa agccggtgct cttgaggtac
      121 cactcgtaga aggcggccct ctcgaagacg tcgaggctgg cggtgtagcc cctgatggag
      181 ctgctgtacc tgatgacgtc gtcggggttc tggacgtcgg cgggcttcag ctccaggtag
      241 taggggtcgg gcaccccttc cgtcccatcg gccccgaagg gtccgtcgat gcctccatcc
      301 ccgtggagga gggcgtaagc cccaatgagg aggaccacgg ccgcgaggac gacccctttt
      361 cttgccattt ctcaagccca cccttggaat atcagttcac taggccccgg tctttgagga
      421 tctgctccac tatcgtccga acctcatcct cgtcgacctt tctgcggccg tggacgtcgt
      481 gggcgccggt accctgcctc gacctctcga agatgttcct ctcgtcgagg aagaagggct
      541 tctctccctc ctcgatcctc ttgagacccg cttcaaaccg cgttcgaatc ccattgtccc
      601 catagaagta gtcggcgagg gccctcttcg tcagatccgg catcccatcc tttacgaggt
      661 ggctctcggg atcgtccatg tacctgatcg ccatcctgat caccctgtga agccgatgct
      721 cgaggttccc cttcacctcg catcccctct tctccccgta agggtttcgc aggatgatca
      781 ggacctcatc ccagccgttc tccccgagct tcctccacgg ctctatccga tccgcagaaa
      841 ggcccgtctc cctcgccacc gccttcccaa tctcctcgag attcatgaac cctctagtga
      901 agacgtaggc cttctctgcc cgccgcggat cgcagccgaa gagcttcccc tccgagaggg
      961 cctttctgac taattcgtcc tcagggtcct tcctcctcct atcccggaag tagcagggga
     1021 cgaggatggg cgggcggtcg agcccctcaa tgaacttctc cctcatcgcc aggctcggag
     1081 ggtcctcccc gggatagtgg cggccggcga cgaatggcct catcgactgg tggatgaagt
     1141 cgtcgatggc agggtagttc tggccgaacc tctccaccag gatctggttg tccatcagga
     1201 tcaccccgtc cacccccacc cccaccttct tggtcaagag gtcgaagagg gatatggagg
     1261 cacccagatc cctcttcgcc tccttagccc tctgctgagg gtcggtcccc tcctccgtga
     1321 gaacccccag gacgaagact ggataggcgg gcctctcccc ccggatgtac ctggttatgg
     1381 ggttgacgaa gccggtcccc gtaccaccgc cgagggagac gatgaagagg attccgtcgc
     1441 acatctccga tccgctgttc tccttcgcag ctgccagggt cgtataatct cttatcctat
     1501 cccaggctgc ccggaggatc gggttctccg acccgttctc cgagagggcg gaacgcttca
     1561 cctcctcgtc cgcctcaaag atctcgaaga cggccttgag aaactcggcc tgcctcatga
     1621 acccctgctt tttgacgtcg tacccgtatc ggtcgacgat cagcttcgag gtcttcgact
     1681 cggacacgat gacgtcatgg ggaatgaagt agcaggggta gcaccccctt tcctcagagc
     1741 cgaagaagct ctggttctcc gtctcctgga cgtccgcctc cagccagagg cccttgacgc
     1801 ctccgaagga gacgtgctct cccagggact tcttgagggg tgcaacgtcc tggtttttga
     1861 ggaaccgctc cgccagatgt cccccacaac cgccgatccc aactatacag agcctcatct
     1921 catccctcct cgggcctcag catatacggg gaagaggagc gccacctcag agccgaagag
     1981 ggctaggaag gcgagccccc tagtagcggg gtccacatcg ggaaatattg tcccctgttg
     2041 aacggaaagg gctatggaga gggctaggac cggcaccgtc agcattatcg cgaccttcgt
     2101 cttcctggag agctcgtagt agccgtagag ttccctgggg atgaaggcgg ccgcaaagcc
     2161 caggggtccc gaagataaag tcggcatctg gtagtagttg tggccaggga atatctcggg
     2221 ggtcaggaag tagaaataag cgaaggcggc gaagaggagc atccaggcca ccaccgccgc
     2281 ggcgttgaag ttcgatccct ggcgggtgaa catctcctct cgaagatcct cgggggatac
     2341 ttcaccgtcc cgggtggtgg ccgccacgtt ccgataggtc ctatccatgt tcagcctgtg
     2401 gaggaggacg cctccggcga ggaggaggat gaatagcccg acgcccagaa ggccgttgga
     2461 gtatatgccg gcgaggaagc ttgatacccc ctcggaggcg aggcctgaag atatatcgac
     2521 ggcctggacc gccgtcaccg tctcgttcag gtcgctcatc ggtacagcct cctctcgatc
     2581 acgatcccac ccgcaagggc tgccagcagt accacgagga ggtttccgat cgtgaccatg
     2641 gagtatatcg gatacctgac gatcttcgga tttatcttcc catcggaggt tccgcccatc
     2701 tcctctttgg agttctggtc cgtcgccgag tacttgaagt aatagctgaa gctctcgtcg
     2761 gcgtcagata cgtcgaaggg ctgaaggtcg aagatcacca cctgcgcctc ctccgaggcg
     2821 ttgatctccc tcgtcgcctg gtacctcttg ggatgggaag gggtgctggt gtagaggtct
     2881 atccgaaggt tatccacctc cgggttcttc accctgaggc tgaaccggta gctctgccac
     2941 cagtaccggt tctcgtcggc ggcctccaca tgggggtccg agacgaagat cttggggctg
     3001 ggaaggaggc tcggcccggg gtatacgcct gtcgcgttcc catcgaggcc gtcgtcgtag
     3061 ctgaaccggt aagagaagtt cctccccgcg tccgcctccc cgaagaggcc gtactcggtg
     3121 gcggagaagg tgacgttcgt ccccgacttc acctcctggg tgaggtttct caaaatcccg
     3181 ccctggtcgt cgaggacgtg gagggtgacg ttcaccatat cgccgtcggt gtcgacgaac
     3241 ccggcggaat acctgatggg gtcgttgtac ctggccctcg ccggctcaac cttgatgctc
     3301 tctcccacga ccaacggggg gctgttgacg agcgagagct ccaggatgtt gaaaaaatcg
     3361 ccctgtctat agggcttatc gtacatggta tcggtgaggt aggtgctgtg catatcgttg
     3421 aaccatatcc ggaaggtggc gttccccacc tccctcgaat cgacgaaagg agtcagcccg
     3481 gaccagtccc tggtggtgga gttggaactc ccgacatact cgttcgccag gatcaccctc
     3541 tgggagccga ccctggtgaa gttctcatcc ccgggtccct tcgcctccag gtagaggtag
     3601 acgtccgacc tgacctcgat gggattttcg ttcttgaccc aggcatatat gtcgagcttc
     3661 tcgttccggg tgatctcggc cttctccgtg gagtttgggt accagataag ggcggtgggg
     3721 gtggagtagt ccttcggcgg gacgaagggt atgtaaagga gcttcttcag gtaatcgaac
     3781 ttctcggggt cgctctgctt ccacttctcc atattaccta tgaggtcttc cagctccagc
     3841 ttgacgatct tcttcccccc gctgctggtg gtggtagaag ctccggcgct gcctccgctg
     3901 gaaccccccc ctgtggaccc gctcggacct cccggcccct cggccccctg gacagacccc
     3961 ataaggtaga agatgagcaa aaatattgct ataaagcctc ttttcatgcc tagatcccca
     4021 ctggctcttc gaatctgata tcaacctcgt agaacctgta tccaggtttg tcctcccagg
     4081 cgagcctcgt ccagccagac cctccagtat aacgtttcca atcttcgagg ttcttccagt
     4141 tctcgttgtc ggtggagtat atcaggtcgg ctatcatgtc gacgcttcca ttgacccagg
     4201 ccacgtagtt ataacgacca ggactgacgt cctcataatc gaggtttttg tacatggcga
     4261 cgatctcggg gccgctgaat accgccgttt ctacgccatt gaccagaaac ttgtactctg
     4321 cagatccgag gaactcgacg tctgtgaagg gcttgagcac ccaggatacg tttccgtttg
     4381 aagaagaata ctgcttccgt tcgcctacag ccttccatac ggagttgggc gccttgacca
     4441 gaagctcaac ccagatctcc cccttatact cagaatcgtt cccaaggttc tcgatatgga
     4501 tcgtgtaaac gtacctgttg aggttattgc tgtcggggaa gacctgcata acacctctgg
     4561 ccggcgtcac atttcctgtg atcgactccg tgacgatgac cggcccctga tagacggtgg
     4621 gaggtgaagc cacccttatg aaccggtagc ttacgttgcc ggcgacgtca ccgccgaagg
     4681 cgacgtcctt ccaggttatc ctggggtcgc cgttgtaggt ggctatcccc tgggacctcc
     4741 agatcccaga tcccggctcg ctcgtctgga gctctatgtc gcagacgggg agggcggtgc
     4801 tgacgtcgac ggcgtaggta taccgggcta cggtgctccc cctctcaggg gagacggtgg
     4861 agttctcata ctttataagg acgatctccg ggccgaggtt caccttcccc tcctcctccc
     4921 ggctcgtccc cagggggctc tcggagccgg ggtagtagaa gctgaagtag taggagctcg
     4981 ccccctccga gcctatcgcc tcgctgggca tgacgccggt ccagacgagc ctctgccaga
     5041 cggtggcgtt tccgtatctc ttcttctcga cgagcttgaa gaggttctgg tctacgtccc
     5101 agatgttgag gcctacgtcc aggttctttg agcccctgaa atcgagggag taggtgaaag
     5161 gagagcccga gaagccgtct tttggggtta cggtagcgtt tgcgtattca tattccaccg
     5221 gccagaaggg cccctcgaag gcctcggtct gctttcttcc gacgaacctg taccagccct
     5281 tgaggaagtc gaagtcgagg gagacgttct cccacctcag ggtctgccat ccgccgatgt
     5341 tggtgtagtt ccggctaccg caatccttcc aggggccgat cgcctccgga gccacctgga
     5401 gcgtcaccgc gtcatcggca ctggagaaga cctcaatccg atagctataa aggtcggcat
     5461 ccgtccccat cttgggggat acctcggggc ttctgtagac cgtgaccatc ttgatgaggg
     5521 agggctcacc gtcgtagctc tccagggtca agggggagcc gacgaggggg gttatcaaga
     5581 ccctgtacca gcccttctcc cagatgctgg tgtcgttatc gtcggtggac cacctcccaa
     5641 aggccccccg gctcatctcc ggtgacctga agttgacgtc gaagacgacg tcctgagctg
     5701 agcgggccgt gaggctgatg tcggtcgtgt acttctttga ggcggagtag tcggtgaagt
     5761 cgggtccgac ctctagctcc accgagtagc gcatggggct gttcatcgca ttcgtcaggt
     5821 gaattttgta ggctatcggc tggttgacgt tgtaaggcat gctggcggtg ctcacctgct
     5881 ggaagctcat ggacgcttcc gtctggccga gggccggagt caggaccacg atggccaaga
     5941 gagagaggac gatcgcagat ctcctataca tcaagctttc acaacccacc tgatttatct
     6001 gcctatattt cacaatctgc ccctgtggct ataaaagata tgtctctatt aaatgttaac
     6061 ggatgaactg gcggtggcgg agacgaaaaa ttttagaggg agattcaagc ctcgatggct
     6121 ttgctcgccg cctcgacccc agccccgagg ctggatatca cgccctcctt ctccaggacc
     6181 agctccaggg tctgaatcgt cctgaggacg tcggcctcgg tgcaggcccc catcgtccct
     6241 atcctgaata tcttcccctt cagcccctcc tggccgccgg agacgacgac gccgcgcttt
     6301 cgcatccctc cgcgaagctg gctgtcgctg atgccggagg gcatcttcat ggcggtgacg
     6361 gtgttggagt aggtggtgtg ctcgttgacc tgggggaaga gctggatatc gagggctgat
     6421 gctgccgctc tcaccccccc ggcgagggca gccatccggg ccctcctctc ggggaagccc
     6481 tcctctgcgg ccatcttcag cgcctcctgg agggcgaaga ataaggagac ggctggagtg
     6541 tagggggtct ggttcttact ggccttcttc aggtgggcct tgaggtcggt ataatagccg
     6601 cccttcccct cctccaagag gtccatcgcc ctcttgctga cggccaccat ggcgagcccc
     6661 ggcggtacgg cgagacactt ctgagatccg gtgatggcga tgtcgatccc ccattcgtcg
     6721 gtgagaaact cgttaccccc aacggaggag atgccgtcga cgatgaaaac ggcgtcgtac
     6781 tttctcgcga gcttccctat ctccctagcg gggttgacga tcccgacgga ggtctcgttg
     6841 tggaccatgg ttatcgcctt ggcgccatcg tccagggccg cctctatcct cgccaggtcg
     6901 aagggggttc cccaggggta gtccagggaa actatggacc cgtaacggtc gccgagctcc
     6961 gtgaacctct cgccgaactt tccgttggtg acggtgacga ccttctcccc tttgccgatg
     7021 atgccgccga cggccgcctc catgccggcg gttccgctgc cgctcaggac cacgacttcg
     7081 ttcttcgtat cgaagaggtc cttcagcctc gacctgcagt cctcgtagat cttctcgaag
     7141 tcggcgcttc ggtggctgat catcggcttc gccatggccc ggagcaccct ctgggggacc
     7201 ttaacaggcc ccgggatcat cagcagcgtg tcctcgattt ccaagtttta tccctcgtcc
     7261 taacaaccac ggggagtcta tataaatcta gcttttatcc gttctgcggt cttccagctc
     7321 tgcctcgcca cgggcggaag gtcgccgtgc tcctccaccc aggattcgag gaagcggatg
     7381 gtggcgggat ctgatggata gccgctaccg atctctcgtc cgatggcctt ctcgatctcc
     7441 cggaccgacc ggtccctcct caccttggcc aggatcgagg cagcggcgac gaccgggtgg
     7501 tttcgatcgg cccggtgctc ggcgacgacc tccatggagg tcttgctcac ctccttcacc
     7561 ctccgggcga accgctccgc cttcacgtcg gcggcgtcga ggatcgccct atccgccgag
     7621 aggttctcca ggacctttga gaagcttctg accatgatct cgttcatgct catgatcttc
     7681 cggagctcgt cgatcacctc gggtctcacc tccaggacgt atctgtcgag ggcgatcttc
     7741 tctatcctga tggctagggc ctccctcctg gtgggggcga ggagctttga gtccttcacc
     7801 cccagggccg caaggtcgaa gagccggtcc tcgtcgatga ccacgccagc gacgaacatc
     7861 gagccgatca ccggcccctt ccccgcctcg tcggcgccca aaagtcgcat cgctcatctg
     7921 gtgggggccg gtggtatttg aatcgatcga aaaatgaggg gatagagacg agagaggata
     7981 gagacgaggt ctaagcggat cagatgaaaa attatatgcg atacatccat catttcattt
     8041 aatgtctaga tcattgtaaa ttatgtctga gaagagaagt ccccggagat atccacgata
     8101 tattggatat aatattaatg ctctatatga ataaaatttt tagagcgcga tggatcgcgc
     8161 tctataggct gaaacggatg gcgttgggcg tgatgtagcc gaagctccgc ggctgggctg
     8221 aggggtttga gaggtcgaag tatacgacgt cgggctcatc atagatcggc gggctcgcca
     8281 gggttttcaa gggggcgcca ggatctatgt agacgttccc gttttcgttg aagaacctca
     8341 gcttccctct agccccgggg taccatacgt cgaagctggg accctggtgg aggacggagc
     8401 attccaggcc ataatccgaa tccccttcct tcaccaggga ggcggggagg ccgtgaacgg
     8461 agctgagcct gacgtcccct gaccccaaaa tccaggctgg agatgcggtc tttatataaa
     8521 cggagtcggt gatgtcgaac tggcctacaa cccccaggac ctcatcgacg aagacgatcc
     8581 tcggaagccc ccggggaaaa ggcgtcagat ccttctccag gtctccatcc cccgccttca
     8641 ccttggatcc cgcagatccg aaggcggaat ctgtgagcct gatgtcgttc gctgatacat
     8701 cagtccccat caccacgact ctgccgatgt ggagatagag gacatctccc tcgtcgaagg
     8761 tcatggagct ggatcccgta tcccagtacc ccatctggac cggggtctga acgaaaggct
     8821 ccggcgacgg aaggttgtcg gcgaagtcct ccacgatgtc tccgacctcg gggctggagt
     8881 tcgtcaccct gtatccgaac tgaccatagg ccattccgac gaggccgagg gccacgacca
     8941 aacccgcaga gatgcaggca gcaattaacc tcttcattct tccctcctca attatggatt
     9001 agatgatccg ccaagtcatc cggtcagacg gacgttattg ggaaccacaa aaccgaaggg
     9061 ataacctcct gccccagcaa aggcgatgct cggcagggat atgtctatgt aaaggtcgtc
     9121 gctgtagtcg tagatcggat ctccgacgcc gtccacgttc ccgttggcgt tgtagaacct
     9181 cagggtggct atgggccccc cggaggagag gggaacgatc atgggaatca ccggccaatc
     9241 gttatcagga tggatgtcca ggatcttcgt ccccggcgcc agacctccga cccgaaccag
     9301 cctgacgtcg tttgtggcgg tcttggccgg ggggaaagag ttcgtcacca gtaagtatac
     9361 cggatcggcg aggtcataac cctggcttcc gtagtggtct gcataataga tgcccgcctg
     9421 ctgccccggc agattgagga gcggcttgtt aatgtcgttg tccgcggagg tcaccttcgt
     9481 ccccgcgagg tacgatccgt agccggtgag ccttatgtca ttggcgtcga tgacgttatt
     9541 tcccaacatg tccagatata tggtgtcgcc gtcgtcgtag atcccggggt ttattccggt
     9601 gtcccagtag ccgaagttcg ggttcagggg tgccggcaga tcctcgagga ggcgcccaac
     9661 gtcggcatcc cccgtcagca ccttagagcc aaactgtccc aaagagctgc tgaagctaag
     9721 ggcaagaacc agcatagtca gcagacagac tttcataaat accatcagtt taccccccct
     9781 tcatatttca aggaacggaa tacattttat cgtaatctta taagtacttt tttgggtttt
     9841 gatgatgcga aatattctcc tatccacccc ttaaatagac acccgccccc caagacccaa
     9901 aggacggcga gaagggcgag aggaggaggc gagatgccgg agggatgaga ggagttcacc
     9961 tctccaggat cgtgcctcct cgaccccctc acctcgaaaa agagtgaggc gaggggcccg
    10021 gaggagcttc aggtggacat tctgacgtcg tttatcgtca ccacgtagaa gggatactgg
    10081 gtgtccatgt agaccacatc ccccctatcg tagatggcgt aacccccgtt gttgatgttc
    10141 ccgttcttgt tgaagaagct gagcatcccc gggagggtcg atgtcttgag gccgttgtca
    10201 ggatcggcgt cgttgaccct gctccccgca gggaagccct tatagctggt gatccggacg
    10261 tcgttcgcgt tgatctgccc cggcaccacg ttcaggtaga tgggatcctc caggttgtag
    10321 gcgagatcgc cccctacatc gaggaaccga agctcagccc tgggggtggt ggcggtcccg
    10381 aacttggtca gctgctttcc tatgtcgttg tcggccttcg ccacctggga ccccgcagga
    10441 agatccccaa agggcgtcag gcggacgtca ttttcgctta ccacatcgtc gctggggttg
    10501 atgtttatgt agactacgtc ttcggtatcg aagacgccga tgatcccctc atcccagaag
    10561 gcgaactcag ggcccgtata gaaggggctg agagctcccg cctcgtccac gtcgttccac
    10621 ttgaccttag taccgtactc catatcctcg ctggctgagg tggtcgaggt ggcgaccaga
    10681 gccaaaacca gcaccatcac cgctgttctg cataaaggaa gatacctcat catgagtcac
    10741 cccacatcat ttcgtattac tcccctcgat tttagattca cctttctcct gtatagcata
    10801 taaatattgc taaaatgatg cctctctatc cggggtccac cccggttgaa cccctcaaga
    10861 tcggggcagc tccgagatga gaagacccac tcagccgatg atttaatccc ctgagatatc
    10921 cggcagggat ccgacgggcc ttcttcccgc cctcgccatc cccgaagaaa gcctccccgg
    10981 gtggggctcg ccagatcccg gccgccatct tccgacctcc atcatctcac gagggtccag
    11041 acccgaccgt agttgctggg gatcgagaga cgggtccaga ccccggcggc gacatccttc
    11101 aggtcgtcct tggcgtggag ctcctcccga taatctccgc cgagagggaa ctggaactcg
    11161 acctcctgat cccgatcggt gaagttaagg gcgacgaggc tgtagcgctt tccgtcatcc
    11221 cgggagaata ggagaacccc cctggactgg tatcggtccg gatcgttgaa gaagtggtga
    11281 cctcccctct ggaactgggg ggaacgccgc atcttgatca gcctccggac gagggagacg
    11341 atcctcttcc ccacgggatc gtagaagtag tcccaacgga ccggcctgta taccaagacc
    11401 cgccccatcc ccctctctgg aatgaagtag ttctccccga actcctgccc ctgccagatc
    11461 atcggtatcc ccttggcggc gaagatcccg atcaggtagg gctggacctt gtaccagaga
    11521 ctccggtccc cgtccttcag ggccatcagg tccgatccga tcgccccgca gaggtcccgg
    11581 ttcctctcct tcaggagcct gaggttttcg tcgatccccc ccgggaggtt gccgaagctg
    11641 cagacgaggc ggggatggtc gtgattctcc aggtactgga gggcggtctt tctgatgtta
    11701 tcgccgttcg cctccgcctc ggcggggtat cccatgaggc cgaggcagaa gccgagggcg
    11761 gtcagccatc cgccttcccc ccgggccacc ttctttgccg cgtccagggt ctcgttctgc
    11821 caggtgcagt tgctgtaggt ctcccggaga acatccctcg gcccctccag ctgctcggcg
    11881 cactggatca ggtttatgga gccgccgtcg aagaacctcc gccagacgcc ggccttagcc
    11941 ttgaccaggt tgtaggtctc gtagacgagg gaggcgtatc ccctccccac ggggccgtcc
    12001 cagtagttgg ggacgcagtc gtaccgaaac ccgtcgacgt ggtatacctc tagccagtgg
    12061 tggttgacgg tgaagaagaa gtcctgagtc atcaagcggt tgaagtcggt gctccggccg
    12121 aagtagtcct tggcgaaggg gcccatgaag gggttctcgg agtagttaag cctctcgtag
    12181 aggtagcagt aggggaactg gtcgctggtg tggccgtaga cgacgtccag gatgacggcg
    12241 atccccctct gatgggcggc gtcgacgaac ctctgcatat cgcacctttt gccgaaccgg
    12301 tcgtcgaccc cgaagtagcc gatgggaaga aagccccagt cgacggtgtt ggagacgttg
    12361 gagaccggca tcacctcgat gcagtttatc cccagatcct ggagatagtc gagcctctcg
    12421 atggcttccc tcaccgagcc cccgaactcg ctgatcatca gctcgtagac gatcagctcc
    12481 tccaggggcg gcgtcctcca agcctcctcc tcatcgctcc attcgtacct atagtcccta
    12541 tcgtaccctt tggtgatcgc cgagagcttt ccgaccccaa actcccgggc gaaggggtcg
    12601 atgatccagt ctatcggctc ttcgaggtgg ggggacttca gctggtacct gtagacgtac
    12661 ctcccgtcgt ctccccagtg ggagcccgga tggctctccg gagagtccgg atcgatctcc
    12721 acctccgtcg accagagggc gccgtaatcg gggtcctccg actgatccat ctcaaactcc
    12781 atcggctgta catcctggat gaactggtct ctctcgtgaa ttatcttgac aatgagccgg
    12841 tttccatctt tggcatagac caaggggagg aggacgccga agtcgaagac ctccggcgag
    12901 gtacccctcg aacgcgcccc gagccgctcc agatgcaaaa gcctcatcta taaaacctcc
    12961 ctatcgataa accggcgaag gaccgtcccg gacctccccg acttatcctc atttatttga
    13021 ggtttcaaga tataagtaag ttttcatata ttgagaaagt acgggaggtg gacgcctcct
    13081 ccggggcgta ggattccggc tatcccgagg agaggcccct gggatccggc ctcacccagg
    13141 ggcgcagacc tggaataggt tgatctatcg gcaggaagag accttcgtac gatgatcgat
    13201 ttgctcctca ggaccctcga ttttgcatcc cccatcctcg cgatgatagg gatcggcctc
    13261 ttcggagccg gggtgctgac ggagatgggg ctgctccggg gcatatccag gatcgcaagc
    13321 cccctcttct ccttcaccag gctccccgac gcctgcgcct cggccttcgt cgtctccctg
    13381 gggtcggcgg tggcagccaa cgggatggtg gcgaggttcc gggacgaggg gtgcctcaac
    13441 gagagggagg tgatcctctg cgccatcatg aacagcatcc ccgtctactt ccgggagctc
    13501 ttcacctatc agatacccat cgtccttccg gccctggggc ttctggtggg gggattctac
    13561 gctttcgtct tcatcgtcac cgccctcgtc aagatatcgg cggtggtgat cctctccagg
    13621 atcctcctcg tcgagaggag ctgccgggtc cccgagccca tccggcagga gaaggtctcc
    13681 ctccgggagg cggtggcgag gtccttccgg cgagagagga ggctctttct caagatcgcc
    13741 gcaatctacc tcaccatgac ggccgcggtc ttcgccctga gggaccgggg ggccttcgag
    13801 gccttcagcg tcctccccct ggcggagatc ttcgggatcc ctccggagag catcgtgccg
    13861 ctgaccacct acgtcgcaag ccccatcgtc gggatatccc tcctggggcc gatgattcat
    13921 agcaacggga tcaccccggt ccaggcgatg atcgtcctga tgctggggag catgttcatg
    13981 ctccccctct tcgccgcaag aagcctcctc ccccgctacg tcgggctctt cggtccgcgg
    14041 cttggggtcg ggatcgtcct cttctccacg gggatgagca tcctcgtcag gctctccatc
    14101 cttctggcgc tcctggcctt cggccggtga ggcccctctc ccccccggcc caggtggtat
    14161 ccggcggccc ttcgcccgcc tatccctcgc cgatgatctg gacgactacc ctccggtacc
    14221 ggggggttgt gtccaggtcg aggaagacta tctgctgcca ggtcccgagg agaagcttcc
    14281 tgccggcgaa ggggacggcg aggcttgggc cgatcaccga ggcccggatg tgggagtggc
    14341 cgtttccgtc cccccacctc tggttgtggg cgtactcccc cccctccggg gctatcctct
    14401 ccagggcgcc cttcatatcc tccacgagcc ccggctcgta ctcgatggtg gtgaccgccc
    14461 cggtggctcc ggaggcgaag acgacgacca cccccgcaga gagccccgtc ctctcgacga
    14521 tggcggcgac ctttccggtc aggtcggcca tctcgccgtt tccccgggtc tgaaagtcga
    14581 tatgctcggt aacgatcaag ggatctcctc cgtccattga agggatctgc atcaagaccg
    14641 agaggccgca cgcctccatc accaccctcg aacccattct ggcggccttg acggcccgcc
    14701 cctagcgccc gacggagccg atggatggag gtgccgttgt tcggcggtac aggaggcttt
    14761 ccggagccgg tccgcatcat cccgaaagta tctggggctc ccacccttga tcaaactttg
    14821 cagaggagga gcagggccga aaggggtggg gttggtccgg aaatccgaga aaaaaatttt
    14881 tgtaccatcg ggaagatata catcccatag gtgttcacct tgaaagcctt tggcctgaaa
    14941 gccctcctcc tcctgaccct cgccctgata atgccgatgg cctcctccat cgacccccca
    15001 tcaggctctt ccatatacag ctccgaggac gcctcttcct tcgccgagtt cttcgcgatg
    15061 gagcctccgg cagcctccgg cgaggtggag aggtacagcc tcgtcggccg ggagccctcc
    15121 tccgtcacct tcggcggccg gtccacgtcc tattccgcct accagtccgc cggcgggggt
    15181 gctaacaccc tctggatttt gggggcctcc acctggaccc agcgggtcgt cgctcccctg
    15241 ggatcttacc tctacctcct cgcctcctct ggcgggggaa gggccgactt ctacgagatc
    15301 cgccccgacg ggacggtctc atccagctcc cgaaccctct atccgggaca taataggctc
    15361 gtcttccacg ccgacgccgc cggacggcac gtcctcctct tcgtcgccgc cggccagccg
    15421 agcaacgtcg tcatcgtcga tgtcgccaga ggaggctggc ctccgtcgcc ggggccggcc
    15481 cccggcccag ggccgtggcc gtctccagga tacgccaggg tgatcttcag ctccacctgg
    15541 ctcaagggct acagcgtctt cgtcgacggc acttacgtcg gcggcgacgg ccagggcggc
    15601 gatcccttag acgggacctt cagcctgaac gtccccgggg accgttacca ggtcctgcag
    15661 atatccgccg gaggccggac ctactcggag cggggtactt tcctctcggg ctacacctac
    15721 aggctctcga tatagcccga atcattcggg gcggccctcc atccgccccc ccaacgtttt
    15781 cccaacgcca acgaggtcct ccggcgttcg accggattaa aacaaactaa cttgatttat
    15841 ccaaaagata tttaaccaat ttagacaagt cagctttaat taaatcaata taaccgacaa
    15901 aggtggaccg atatgctcag aaccgcgatg atcctgctaa ccctggcgct cctatcggcg
    15961 ccggccctat cccatgacgc atcaggccag accctcaccg tcaccgatga ggaggggagg
    16021 gaggtgaccc ttcccgcgat ccccaagaag atcatctctc tcaccccggc ggcggcggag
    16081 gtgatatacg ccctcggcga ggcagatcgg ctggctgcgg tatcggagga ctgcatcagc
    16141 cctcctggcc tgatggcgat ggagaaggtg ggagagtccg gccgggaggt ggacctggag
    16201 agggtcatgg agatcggccc cgacctggtg atagcgaaga ccggggggct cttctcggca
    16261 gaggacgagg agcggctgaa ggggtacggg gtccccgtcc tcaggtatcg ggggcttcag
    16321 atcgacaccc tcatccccat gatcgccgac cttggcctga tcctggggag ggaggaggag
    16381 gcgagggagc tgatcggctg ggccaggggc tacagcgacc tcgtctctgc caggaccgcc
    16441 gggatcccgg aggaggagag gccccggatc tacttcatgt ccatgggcca cttcgactgg
    16501 accgccggct tcgagtcggc ggggcacgag aggatcgtcg aggccggagg ggagaacatc
    16561 gcggaggatc tgccggggaa ggtgcctcac gtcgatatgg agtgggtgtt agagagagac
    16621 ccggaggtga tagtctactc gatccccacc tcccagtacg ccgggccgac cccgaacctc
    16681 caggagatgg cggataagag ggcggagatc atgagcctcc cggccttcga ccagatcgat
    16741 gccgtcgaga ccggaagggt ctacgtcgcc gacataaaca tggccagcgg cctatctgag
    16801 gtcgtgatga tcctctacta cgccaagtgg ttccaccccg acctcttcac cgatatcgat
    16861 ccgcgggccg tctctgagga ggtctatcgg agattcttcg gtatggagat caacgccgtt
    16921 caccaggtct atcccgagac cgcaatctta taaacggccc ggggatagag gctgctccat
    16981 ggtctccaac ttgaatctgc atggctgggt cgaggttgat gggaggcggc tcacggtgct
    17041 ggaggtggag aagatcctcg aagagagccc agccacctta tccgagttcg gaggcgagtt
    17101 cttcctctcc tgggatgggg ggaaggcccg ggaccatttc gggatcgtcc ccggggactg
    17161 tccccccggg aagatcgtct gcgacggccg agttgtcgga gttgtcgatc cgcagccgcc
    17221 ggctgcggac ctccccgggg cgatcgagac cgccgtcgtt ctgcgaaaag acgagggggt
    17281 cgtcgccctc tccggcggcg tcgactccgc cctcgtcgcg gccctggccg ggagagaatg
    17341 cgtcgttgtg ggggtggagg gttcttccga cctgaagagg gcgaaggagg tcgccgacgc
    17401 cctcgatcta cagctggagg aggcattgat cgaccccgga aggatcgaag aggccctggc
    17461 cgccgttctc cccgccattc ccagcagaaa ccccgtcgac gcctccatcg ccgccaccct
    17521 ctactttgtg gcggaatggg cctctctccg cggccacgag aggattctgg cggggcaggg
    17581 tgccgacgag ctcttcggcg gctacgcccg gtacctggag acgacgaccc tcgccgcgga
    17641 tctggagcgg gacttctccg gccttcccct ccagctggtc cgggaccagg cggtggccgg
    17701 cctcttcggc gtgcgcttct ccctccccta cctggacctt cgggtggtga gggcctccat
    17761 ggcgatcccc cccgaggaga aggtcaaaga aggcgtccgg aaacggcctc tgcgggaggt
    17821 ggcggcccgc cacctgccag ccgaggtcgc ctatcgggag aagaaggcga tgcagtacgg
    17881 gtcgggggtg atgcgggagg tgaagaggct cgcccggagg agggggcacg ggaagatggg
    17941 ggattatctg gacgggctgg catgaccggc cccgcaccgc atcctttttg aggcgagaac
    18001 ccctgggact ataggtgggg tcggtcttgt cttcagtctt ttcgctatcc ccgggaaggc
    18061 accttctctg gagcccggat cacggatcgg atatagtcgc ctccataata gacctggccg
    18121 agaggctgga ggtgaggtgc ggaacggtcg ccgccatcgg ggctctgacg gaagcgaccc
    18181 tgggtttcta cgaccaggag gcccacgagt acaggacgat cgagatcgac cgccccggtg
    18241 agataacagg cttcctcgga aatatctccg tcagggacgg gcggcccttc ctccacgccc
    18301 atgccaccct ggcggactcg aaaggagacg tcatgggcgg ccacctcacc cgcgggacgg
    18361 tcttcgccgc cgaggtctac ctccaggagc ttttggggac gccgccggtg agggtccacg
    18421 accccaacac cgatctgttc ctctggagga gcggcgattg aactccaccg agtacttcga
    18481 ggtgctggag gcggggctcg cctccgggat ggaggtcgcc ggcagagccc gacgccaagg
    18541 caaagatccc agcctctcgg tggaggtgcc gacggccatc gacctggcgg agagggtcga
    18601 gaaactgatc gggatcgagg ggatcgccgc cagggtccgg gagctggagg cggaggggat
    18661 gagccgggag gaggcggccc tggccatagg ctccgacttc gccgcaggaa ggatcgggag
    18721 cttctcgtcg aagatcgagg cgatcgacgg cgccatccgg acctccgtcg ccctcctcac
    18781 cgagggggtg gtggcagccc ccatcgaggg gatcgccaag gtcgacctgg ggaagaacga
    18841 cgacgggacc gagtacctca aggtctacta cgcggggccc atcaggtcgg ccggggggac
    18901 ggcccaggcc ctctccgtcc tcgtcgccga ctgcgtccgg cgggaggtgg ggatcgaccg
    18961 gtacaagccg aggcccgatg aggtggagag gtacgtcgag gagatcggcc tctaccggcg
    19021 ggtcgcaggc ctccagtacg ctccgtccga cgacgagatc aggaccctgg ttcggaactg
    19081 cccgatctgc atagaggggg agccgacgga ggaggaggag gtctcggggt tccgggacct
    19141 ggagaggatc gagacgaacc gaatccgggg cggcgtcgcc ctcgtctccg ccgaggggat
    19201 cgccctcaag aggccgaagc tgaagaagca cgtgacgaag ctcgccatcg agggctggga
    19261 ctggctcgac gagctcgcca gcggcggaaa gaaggatgga ggggcctcga gcgagaagtt
    19321 cctccgggac atcatcgccg gaaggccggt cttcagccac ccccaccgcc ccggggggtt
    19381 tcggctgagg tacggaaggt gccggaatac cggccttgca gccgccggct tcagccctgc
    19441 gacgatgatc ctcctccggg atttcctcgc cgccgggacc caggtgaagg tggagctccc
    19501 ggggaaggcg gcggccgtct ccccggtctc cagcatcgag gggccgacgg tgaggctcaa
    19561 aaatggcgac ctcgtccggg tcgactccgc cgaggaggcg gtcctcctcc ggggcgaagt
    19621 ggctaagatc gtcgacgtcg gggaggtcct gatcagcttc ggcgactttc tcgagaacaa
    19681 caggaccctc gctcccggat cctactgctt cgagtggtgg gcggcggagc tcgccgaggc
    19741 ggggggcgac cctgctggcc tcgaggcgcc ttccggcgag gaggcgatag agatctcgag
    19801 ccggtggagg gtccccctcc atcccgcctc cacccaccta tggcacgacc tctcccccga
    19861 agggtacctc aggctgaggg aggcggtctc ctccgccggg gagatgaggg acggagccct
    19921 cctcctcccg ccatcggtga aggaggagct ggagaccctc ctggtcctcc acaaggtgag
    19981 ggaagaaagg ttgatcgtcc ccgaccctgc ccccctcctc ctctgcctcg gggtcgaggc
    20041 cgaggggctg aggctccggg agcttcccgg agagccggtt tcagagatcc gggatcaagg
    20101 cgccgacgga gacgcgatcg tggggggaga gcctgcgcct cggacctccc tcgacctggt
    20161 gaaccaggtc gccgggatca gggtgatggc gagggccccc accagggtcg gctcgaggat
    20221 ggggaggccc gagaagtccg acaagaggga gatgaggccg cccccccacg tcctctttcc
    20281 caccggcgat gccggaggaa agagccggtc agtgggggag tgcgccaaaa accacgtcgg
    20341 aaacggcaag gtcgggatca tcgagacctc cataggaaag aggggctgcc cccagtgcgg
    20401 cgccgagacc cacgagttcc tctgccgatg cggcgcccac accctcccga agaggagctg
    20461 ccccgtctgc caccagccag gaaaggagaa gtgccccagg tgcggaaggc cgacgaccgc
    20521 cgccgatgag atgagcctcg acgtcaaaga gctctaccac cgggctctcg cgcacctcca
    20581 cgagagggag ccggagctgg tgaggggggt cctcggcctc accagccggg acaagacccc
    20641 cgagagcctg gagaaggggg tcctccgggc gaagcacggg gtctacatct tcaaggacgg
    20701 gacggtcagg tacgacctca ccgaccttcc cctcacccac ttccgccccc gggaggtcca
    20761 cgccgacgtc tcccggctcc gggagctcgg atactccgag gacatctatg gcgatcccct
    20821 gagggacgaa gagcaggtcc tggagctcct cgtccaggac gtcatcctct ccttcgacgc
    20881 cggcgagtac ctcctgaagg tggcggcctt cgtcgacgag gagctggaga agttctacgg
    20941 ccttgagagg ttctacaacg ccagggcccc cgacgacctg atcggatgcc tgctggtggg
    21001 gctcgccccc cacacctccg ccggggtcct ctgccgtctc ataggctaca caacggcctc
    21061 cgccggttac ggccacccct tcttccacgc cgccaagagg aggaactgcg acggggacga
    21121 ggactgcgtc atgctcctca tggacgccct cctcaacttc tcgatgtcgt acctcccgga
    21181 gaagaggggc ggcaaaatgg acgcgcccct ggtcatgacc accaggctcg acccctcgga
    21241 ggtggacggg gaggcccaca acctcgacct cccctccagg taccccctgg agttctaccg
    21301 ggcgacgttg acgggagcgt cgccgaagga ggtggagggg ctcatcgacc tagtctcgaa
    21361 gaggctgggg acggaggccc agtatagggg attcaaattc acccacgaca cctacgacat
    21421 cgccgcgggg ccgaagaaca gcgcctacaa gaccctggag acgatgatag ataagatgga
    21481 cgcccagctc gccctggcga ggtcgatccg ggccgtcgac gagagggacg tggcgacgag
    21541 ggtgatcaac tcccacttcc tccccgacct catcggaaac ctccgggcct tctcccgcca
    21601 gaaggtccgg tgcgtcaagt gcggggcgaa gttccgaagg ccgctcttat ccgacgtatg
    21661 tcccaagtgc ggcgggcggg tcatcctcac cgtccacgag ggtagcgtca gaaagtacct
    21721 ggaggtctcg attaaggtcg ccgaggagtt cggggtcgac gactacacca agcagaggct
    21781 cgagctgatc aagatggaca tcgactccct cttccagaac gagaagtcga agcagacggg
    21841 gcttgcggac ttcatgtaag agaggcgaaa ggaggcctct gcccttaaaa tgaagtccgc
    21901 ctttaaaagg tccagcctca cattatcacc ttcatggcgg gccttgaaaa gcgaagcatc
    21961 tctcgcgggt ttctcttgag ggtggggtcg ccgtagatgg agagctgggc tgcggccggc
    22021 ttctccgaat cgcccgccct caagaccgat tcccgcatca gctcgaagag ggcgtctccg
    22081 atcctgtagg ttccctgggg cccggtcccg gatatgtaat ccgggaagcg ggcggcgaag
    22141 tggctgggag tcccctgctc tgtgttcctc gcgacgaagg ccgccgctcc ggcccgcaga
    22201 aactccgttc cgaagctctg acgatcccgg tagagaaggc cggtgttgca ggagacgtag
    22261 ccggttatca ccgggtggtt tgttgcgaga tccgcgtcct cgacgttctc taccctgaat
    22321 atcgggacgc gatcgttggt ggaaaaggag tcggggcttc cgtggcccat gaagacaacg
    22381 tagtcgctgt tcgatatcag gtcgaaggtc tcttctctca tgctacagcc gtcgcagacc
    22441 ccgtaggggg gcgtcgcccg gcaggggccg caggatgcgt caccctcatc gcaggcgtcg
    22501 ccgaggccga tcggcgggga gagccagcac ctggtgctga accagacctc ggggctcagc
    22561 ccgaagccgc cggattcgtg gaggttgatg gcggtctcca gggccgcgac gatccccgag
    22621 gactccgtcg agaggtcggc aagcctgctg acgacgaacc cctcgtctac gaccccgtct
    22681 tcgtcgaagt cgacgtacca ggcgtcggtg gggaggtacc agatggtgcc atcgatggtg
    22741 acggtgaggg gcctcgggac cgccgcctct cctcccagga ggattatgta ggcgggcccc
    22801 gtctcctcag atatcgtctc cagggcgttc ctgatctcgt gccagtctgt aggatcggcc
    22861 acgcgggcgc cgtaatccct caagcactcc ggcgagtcga ggacgatgta ccttgcatca
    22921 agcccgtcgg tccggtcgac cgtctcgatg tacccgtcca cggcgtcttc gagggctgcc
    22981 gtatccctca agaggctggt cgtgacgagg aggacgtacc ggggccggtg ctccataccg
    23041 acgtcgatca ccctcgtgct ctcgctggtg acggtgatct cccggacgat cccgcccgcc
    23101 ccacggaccc ttttagtgta gggggcgcgg acgctgacga gctccgggct gctcgactcc
    23161 atgatgtagt ccctgtgggc cgttatgtac gagtagctcc cctccgggac cgccacccga
    23221 aagctcccgg agctgccggt ggtcgccgtc caggagcgcc gatcgtcgcc tcggagaaag
    23281 gtgacggcga cgcccgggat cgggtcaccg gtctcctcgt cggtgaccct cccctcgaag
    23341 gcgcacgggt agattacggc tctggcgtcg gcggtcctcg acggcacggc gacggccagc
    23401 tccgaggtgg tcttatcgaa ggatgcaggc gctccggcgg ccgtgaaaac cgtcagcgat
    23461 aaaaggaacg ccacgagagc gagaagatga gccgaacttt ttttcatcag agtcaaaccc
    23521 ccgagagatg aggagcccag cctttatgct cggcttcggc tctcatctgc tcgaatatct
    23581 cttgagacca agatattaac agtttttcgt gatggagggg ggcggtctca caaggcggat
    23641 cgtcggacca cctcatcccc tcccccggat ccacctccgc ggatcgagcc tcccccgcca
    23701 ccgggcgacg acctcgcggc cgaggatgaa gcctcctacc cagaaggacg cctccccgga
    23761 tacgacaaga aaggacgaga gggcgacctt tcccgaggtg gataggggcg ccgatgggac
    23821 gaggaggagg cagccgtaga ggataaagga taggaggacg aggccgaagc cgagcctccg
    23881 ccgccaaccg ccgccgcccc tgaaaaagcc ggcgcgtcca ccctccggcg gagaagaagc
    23941 cgccgagggg ccgcatggca cgtcctccgc cggaggcggt ccgctccaag ccgtcgtcat
    24001 atcatcaacc tttgacgacc tcagatcgag accgtcacct catctcccgg agggccgcca
    24061 gggcagaatc gacccttccc ctcatctcct cgacgaggcg gccgttctcc tcgatcctcc
    24121 tcctccggtc ggcggccatc ctcctcgtct cctccggcgc agggccccca atcctcgacc
    24181 ggacggccac gttcctcctc gggtcgaggg cggccgcaag ctcctcctcg ccgaagcccc
    24241 gttcgcttgg cctgaatttt gcgatcctct ccgccgcccc gtccagatct tctatcgccg
    24301 gcgttccgcc cttcgccgcc agggcgccga cgatctggtg ggcggtccgg aaggggatct
    24361 tcgtcttccg gaccaacgag tcggcaagct ccgtcgccgt cgagaagccg gccccggagg
    24421 cggcctccag ccgctcttta ttgaacttcg ccgacgtgat cgcgcccgcc aggatccgca
    24481 cggcgcccct ggtcgtcgag atcccgcgcc agaggttcgg ggtcgcctcc tgcaggtccc
    24541 ggttgtagct catgggcagc gccttgacga tggcgagggc ggcgacgagg gacccaaaga
    24601 cggtgccggt cttcccccgg atgagctccg cggtgtccgg gttcttcttc tggggcatga
    24661 tggaggaggt cgatgtgaag aggtcatcga gctccaggta gccgaactcc gacgtcgacc
    24721 acaagatcag ctcctcggcg aggcggctcg cgttcaccat caggatggcc gaattggata
    24781 aggcctccag gagaaagtcc ctcgtcgata cggcgtccat gctgttctcg accagcccct
    24841 cgaagcctag gagctcggcg gtcctcttcc tgtcgatatc aaagccggtg gaggcgaagg
    24901 cggcggcgcc caggggcgac ctgttcaccc gctggcaaga gtcccgcagc cgctcgaagt
    24961 cccgcaagag agcgtcggcg tgggcgagga ggtggtgggc gagggtcgtc ggctgggcgt
    25021 gctggaggtg ggtaaacccc ggaattactg tatcggcgtt ctcggcggcc ctctcgacga
    25081 gggcttctgc tagatcgttc acctcggcca tgaggccgag gagctcgccc cggaggacga
    25141 tccggatgca ggtcgctacc tcgtcgtttc gagacctgcc ggtgtgcatc ctcccgccaa
    25201 cggccccaac cctcgagatc agctccgcct cgatcgcctc gtggacgtcc tcgccctccc
    25261 cgagcctcgc cagctcgatg ccgtcgaggg cggcgacgat cgccgagaac tcctcggcgg
    25321 cgatgagctc ccgctccttc agcatcgcca ggtgggccct gtccaccagg aggtccgcct
    25381 cgaagatctg ccggtccgca tccctggagg agatgaaccc caggacgtcc ccggagatct
    25441 cgccgagcct ctctcctctc aaaagcatga ttccatcctc aaactcaggt tgtataatcc
    25501 gcgttgatct tgacgtagtc gtaagagaga tcgcagccga aggcccgggc ggtgccctcg
    25561 ccgaggccga ggtcgagttt gatcagaagc tcctccgacc tcatcgtctc ctccgcctcg
    25621 gagagaaccc catctacgat cttgccgtcc ctgacgagga agacctcatc accgccgacg
    25681 gcggatatcg agagggtgat cctctcgggg tcgacctcgg cgccggacct cccggcggcg
    25741 gccacgatcc tcccccagtt ggggtcgttt ccgaagatgg cggtcttgac gaggctgctt
    25801 ctcatcaccg tcttggcggc gaggcgggcg tcctcccttt ccctcgcccc ggtgacgacc
    25861 atctccacca gcttcgtcgc cccctcgccg tcctttgcca tcatcttcgc cagctctacg
    25921 gagacgtaat tgagggcctc ccggaagtcg tctacatcgg ctccgacctt ccccgtcgcc
    25981 gtcaccagga ccaggtcgtt ggtgctggtg tccccgtcga cgatcagcat gttgaagctc
    26041 tcatcgaccg ctccaaggag gcactcccgg agctcctccg gcgagacctt cgcgtcggtg
    26101 tagatgaagg agagcatcgt cgccatgttg ggctcgatca tcccggcgcc cttggtgatc
    26161 cccgccaccc gaaacccgcg gtgctcgacg gcgatctctt ttacagcggt atccgtcgtc
    26221 atgatcgccc gggccgcctc ctcgctcgct ccggggtcgg accggagcct ctccttcgcc
    26281 tcttcgaaga gcccgacgat cacctcccta tcgaggtacc ttcctatcac ccccgtcgag
    26341 gcgacgccga tccgatccgc cccgaccccc aggtggcctg ccagaagatc cgccatccac
    26401 cgggcgtcct cgaccccccg ggggccggtg taggcgttgg cgcagccgct gtttgcgatc
    26461 accccttcaa gcctcccccc ggagagggcg agatttccgg cggtgacgtc caggggcgcc
    26521 gccctgatcc tgttcgtcgt gaaggccccc gccgcagggc ctgaggcggc gatgagggcg
    26581 acgccgtact tcccccgctt gaccccggag gccctgacgc cctcgacggc gcatatccct
    26641 ccctcaatcc tcttcaactt cctccgcctc catccccaga cctgcgatga tgtcgcccct
    26701 cgtcactatc cccaccagct tcccctcttc caggaccggg aggcggttga tccggtggcg
    26761 gaccatcatc gacgccacct tctcgatcga gtcctcgggg ctgacggtga agaccttctt
    26821 ggtcatcacc tcggataccc gcgtcatccc cgcctcctcg gccccggcct ggagcttctc
    26881 ccaccttatc aggtcgcgga tcggcacctc caggacctcc agggggctgg ggagccagag
    26941 ccccccctcc ctctcgacag agagcatccg gagaaggtcc gactcggaga cgacccccgc
    27001 cagccggtcc ccctccagca ccggcatccc gctgatctca tttgtcctaa gaagcctgac
    27061 ggcgtcgctg acggcttccg tcgcctggat ggccaccggc tcggagttca tcacgtctct
    27121 gatcttcatg ctcctccacc tcagagctct ctatttattt accctttcag tcccgccctt
    27181 cagggcgctc cgcccgggag ccagagcccc tccgtctcgg cgatccccat catcaggttc
    27241 atgttctgga tcgcctggcc ggaggccccc ttcaccaggt tgtctatcgc cgatacgacg
    27301 acgatccgat ccccggagcc gtccacctcg aagccgatgt cgcagaagtt cgacccccgg
    27361 accgctccga gggaggggac gccgggaaca agccggatga agggcgagtc ccggtagaaa
    27421 tcccggtaga tcttcgatat ctcgtccctg tcggggatcg agcctatgaa ctcgtccttc
    27481 accaggaggt gggccgtcgt caggatcccc cggatggcgg gtacgacgtg gggggtgaag
    27541 ctgaccttga tcccggggga gaggcgggat agctcctgat ctacctcggc cctgtgccgg
    27601 tgggtggtga gcttgtaggc gatgacgttc tctgccatat ttgggtagtg gctcgtctcg
    27661 gagggggcgt taccagcccc ggagatcccg gacttggagt cgaagacgac cctctcggcg
    27721 aggccggctc cgacgagggg agccaccgag aggctcgccc ccgtggggta gcatccggga
    27781 tttgcgacga acccggcgcc cctcacctct gggtggagct ccggcatccc gaagaccgcc
    27841 tcccggaagg cccggtgctt catcccgtag gtcctctcga agacctccag ggggagcctg
    27901 tagtcggcgg ataggtcgac gaccttagcc ccagcatcga ggagatcggg gacccagtcc
    27961 atcgccgtcc cgtgggggac ggcggtgaag acgacgtcgc acctctcggc gacctcggat
    28021 gccgagggcg attcgaagtt gagggaggat atcccccgca gatggggatg gacatccccc
    28081 acgagctttc cctccagctt tctggaggtg atgcagaccg cctcagcccc ggggtggagg
    28141 ctcaggaggc ggaggagctc tccgccggtg tagccggacc ctccgactat tcctaccttg
    28201 atcatgggag atggcgagta cgggctggca taaaaacgac acgctggcgt cgagaaaggg
    28261 tccggccccc ggcggggaga agatgttggc agaggaagga cggggccggg gggtgggcta
    28321 agaagaggat tccttcatcg aaggccgggg cagatccggt cggcgaggat ggcgaggtcc
    28381 gcggccacgc cctcgatcct ctcccgatcc tcgagccggg agagccacct ctccgggatc
    28441 cattccgacc cccgggccgc ccccgccagg gccccggcga tggaggcgat ggtatcggtg
    28501 tcgccaccag cggaggcggc ggctatcagc ccctcctccg gctcgaatct tgcgtagcag
    28561 aagaaggcgg cgggaacggt ctcgttcacc atcggggacg tcccgatctc cgccagcgcc
    28621 tcctccggct cgaggtccag aagctccttc gccaagagaa gcctcctgcc gaaggcccca
    28681 tcgaccctct cggaggcgga ggcggccatc tccagaaccc tgaggggggc gaagccgagg
    28741 agggagaggg cgacccccac ggcgacggca accgaacccg ccctcgctgc cgcagaagag
    28801 tgagtgggaa ggctttggag gtcggcgtac ctggcgacga ggtcgaggct gcagtggtag
    28861 acgaggccta tcggcgccgc cctcatcgcc gagccgcagg tcggggtcgc cacccccgcc
    28921 tcccgccagc ccatacctcc caggagctcc tggatcgccg tcttgctcgt ccagccgacc
    28981 ccccgattca tcctcggatc ggcggtccag cacctgcccc aggaggcgag cttcgaggcg
    29041 aaccgctcga cgtcgaagga tccggtctcg atgagggtct ccgccagaat gagggtctgc
    29101 tcggtgtcgt cggtgtactg ccccggagag aggccggagt ggaagtggtc tgcggcgggg
    29161 gggatcatat cccggacctc gccgggcccg gcccggatct cctcggcggt catcccctcg
    29221 acgggcatcc ccagggcatc gccgacggct gcccccagga ggcagccgca aaaccggtcc
    29281 aagagctctc ctgccatggc ggagagaagg cggctccggt actaaatagt catcgaaaac
    29341 gggctcctta gggcggtaaa ggaccttgag gatgggaagg gggggaagag aaggggaggg
    29401 ggtggaggag aggaagggag ctagggaggg gaggggacga agggggggag ggaagggctt
    29461 gagacaaacc atcactccgg agtcgtccgg tctagcctca acctgctcct tccgtccgtc
    29521 gatggccctg gacctctcca ctcggggggg ctctcccggt ctaggaatag gaggattccg
    29581 caccctacaa ggcacatcag cccggtgagg acgaaggtgg cggcgaagcc cgccacgctg
    29641 gcgatggctg caccgccggc ggcggtggcg gcggtcccca ggcccgtgga ggtggaatag
    29701 accgaccact cgaagccctc gctcccgggg ttcaggttcc ggctccagag gcagacccag
    29761 gtggggtatg caaggccgct tcctatgccg tagaggacct ccgccaggta gacctggtat
    29821 atgtggtcta tgaagatgta catcaccggg acgatggcga tgaggaccgt tcccaggata
    29881 agccatctgg tcttcttcac gctaccgtcg acgtaccggg cgaaggggag ctggacgagg
    29941 gacttggtca ccaggaagag ggtgctcgcc acccccgcgg tgaagatcgt ccccccgacc
    30001 accccctcgt ttatgaagat cgccaggatc ggctggatga ggccgaagcc ggtgaggacg
    30061 aagatgtctg atatcatgag gagctttatg gtgcgattca tatttttcac ctcctatttt
    30121 tcaatgcgaa cttggagtat aatctacaga aggcggtgcc ccaagatctc agagaggacc
    30181 tgatcggaga gaaggccaga tccgatggaa cgccggaaca gccttgggtg gcgatgggtt
    30241 ctgagatcga ggaagatatg agcagagcta aaagagctca aaaatggcgc actacaagcc
    30301 agagcggagg cttatacagc ggaggatcgc ctcgtcgagc gtcatcatga agttgggacg
    30361 aaggggttgc atatatagat ttctctccat cgaaagggaa tgatctgtag atatattcgt
    30421 cttcgggata ggtatattct cgttcggtca gccttcaggc ggccttcgat ggaggccgcg
    30481 gccgcctccg gaggtcagcc agccgaggag atcccagctc ctcggccctg agcttaaaaa
    30541 tgctgggacg gagtgggatg gagcaatttt atatttnncc cctctgatga aaggaagatc
    30601 gggataggtc gaggcctact tgcgagctgg agaggctgaa ggccgccggg aggtgtaaaa
    30661 agtcaagccc aaaaacatta aaattacact aaacacctga aagcaagtta ttttacatca
    30721 cattaaaaag atatacacct gtttaaacaa cataaattcg gaaaaagcta aatagatgat
    30781 agtgataact tttgtcatat cacttcgaat ttggaacgat aagagaggtc acaaatatgg
    30841 gtaagaacgt cagcgagagg agcatagatc ccgccacccg ggcgatgctc ctggaggcnn
    30901 nnnnntgctg ccgcaactgc aacatggggc cctgccggat cgaccccttc ggcgaggggg
    30961 cggatcgagg ggtctgcggc gccaccgccg acatcatcgt cgcccgaaac ctcctccgga
    31021 tgatcgccgc cggggcggcg gcccactccg accacgcccg ggacgccgtc ctcgccttca
    31081 agaagatggc ggagggcgaa gccgagggct accaggtcaa agatggggcg aagatgaacg
    31141 ccctggcagc cgagtacggc atctccccgg agggtcggag ccctgaggag gtggcgctgg
    31201 agctcgccaa cgtcctcctc tccgagttcg gaaagcagga gggaccgatc acgttcacca
    31261 ggcgggcccc cgccaagagg cagtcggtct gggagaaggc cgggatcgac ccccgggggg
    31321 tcgaccggga gatcgtcgag tgcatgcaca gaacccacat cggcgtcgac aacgatccgg
    31381 tccacctcct cctccacggc ctcagaacga gcatctccga cggctggggc ggatcgatga
    31441 tcgccaccga cgtccaggac gtcctcttcg gaaccccaac accggtcatg tcagagtcga
    31501 acctgggggt cctgaagtcg gacgaggtga acatcatcgt ccacggccac gagccgnncg
    31561 gagatgatcg tcgccgccgc cgaggacccc gagatgatcg ccttcgctaa agagatgggc
    31621 gcaaagggga tcaacgtatc cgggatctgc tgcaccggaa acgaagttct gatgcgccac
    31681 ggtattccga cggcaggaaa cttcctccag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31801 nnnnnnnnnn nnnnnnnnnn nnnnnnncga ggagaaggcc ctggagacgg cgcgggagat
    31861 cgtccgggcc gccgtcgaga actacccgaa ccgcgacccc ggtagggtga acatccccag
    31921 ggagaagagc ggctgcatgg tcggcttcag cgctgaggcg atcctggggg ccctcggcgg
    31981 aacgccccag cccctcgtcg acgccatcgt ctccggttcg atcaagggcg tcggagccgt
    32041 cgtcggctgc aacaacccca aggtccagca cgacttcggc cacgtaaacc tggtccggga
    32101 gctgatcaga aacgacgtcc tcgtcgtcac caccggctgc aacgccatcg
//
LOCUS       DASM01000178           18664 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_136462, whole genome shotgun sequence.
ACCESSION   DASM01000178 DASM01000000
VERSION     DASM01000178.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 18664)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 18664)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..18664
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_136462"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         4077 a         5396 c         5277 g         3912 t
ORIGIN      
        1 atgcgaaggc caccgagggc agccccgcct gcggcgacat ggtctccgtc tacatcaagg
       61 tggacggtga gacgaagacg atcactgaca tcaagttcca gtcctacggt tgcgcctcga
      121 acatcgccac gggatcgatc atcactgagc ttgcgaaggg aaagaccctg gaggaggcga
      181 agaggatcac ctggaaagag gcctccgagg ccctgggggg gctcccaccg atcaaggccc
      241 actgctcggt cctggcggtg gaagggctca ggtcggccat cagggactac gaggagaggc
      301 acggcctggt cactgagaag gtcccgacga cccgggaggt ggtggagagg cggctgaaga
      361 aggtgatgaa cccgctatcc ggcctcgacc tggtgaggac gaagctcgtc gatgagatcg
      421 aggtgaagga cggcaaagtc agggtggtga tcgaccttcc gcccgatcac cagtttgcga
      481 gggccatccg ggaggaggtg gtcgagaaga tcgagcccct ctgggacgtg gaggaggtgg
      541 aggtcgagtt caaggactga agagtcggat gacttgaaga acatgacgac ctgatggccc
      601 agccggcttt gagggggcaa aatcgtggat agtccgatgg atagctatat ctaaaccggc
      661 ctcgataaat tttggtcgat gaaggtcgat ctggatcaga ttctcgccct cgccggggac
      721 ctggcggagg agggagcgag ggaccggttc cgccgattcc tcctcgaatc aacgaaggac
      781 tccgactcga tgagacagct cgctgacgag gcctcgaagg agcaggaccg tcagcaccgc
      841 ctcgcccagg gggatctcgt ctggagtctc ggcgtccccc ttggatttga gccgagcctc
      901 tcgttcgaag gagtttggat ctccccctcg ggggtccacg tccttctgga gatggtggcc
      961 gggggcggaa cgccggagat cgcctccatc ctcggcagga tcaaaagcct ggtctcagcc
     1021 tccacctcca ccgccgcccc caaggcggag gaggttgctg gaggagagtc ggtcacggca
     1081 aaagatgggg acgtcgggga aatggaggga ggggaggaag aagacgacga ggggctgagc
     1141 gccttcggcc tcctcatcgt cgaggactcc gagatcctcc gggtggagga cgccatcctc
     1201 gatgaggacg aggaggggca ggttcggacg atctcgatct cagccctcct cgccctatcg
     1261 aagatcgcca aagactatca tctcacccac gaagacatac ttttgctact cctcacctca
     1321 ggcccatccg tcgaccccct catcgacctc atctcccggg tcatctctca gtgcgaggcc
     1381 gagaccccga ccctggagga gatcgcccag atactggggc ctctggggcg gggcggcgag
     1441 tccacccccg gtgaggcgct ccaggatctg atcgaccaga gccagtttta cgccttcggg
     1501 agccggggct ctggtcggag ggagatgagg ttgtgaggcg ctcattcaaa atataaggta
     1561 agacttcgct gctggcatga gggtgggcca gtggcgccga aaagctggat cgcctccatt
     1621 cgatccatta cagcgctctc aattgaagta taactcagta atcgaactcc aaaatctcgc
     1681 tcctgctgtc gtcgacgttc cgggtggtct tttatgagaa ccggcaccgc aaacctgccc
     1741 ctccacgggg ggagggctcc cccctggctc ttcaagagga tggtgagcct ggcggagggg
     1801 gtggtggacg ccgtgatctt cgagtatggc cccgaggagc tcctgcggcg gctatccgat
     1861 ccctgctggt ttcaggccct ctcctgcgtc ctcggcttcg actggcactc ctccgggacc
     1921 acgaccacca ccaccggagc tttgaagctc gcgataaagc ccgaggtcca ctccgttggg
     1981 gtcgccggtg ggaagggaaa gaccgggagg aagactcccc tcgatatcga gactgcgggc
     2041 gagttcctcg acctctctcc ggagaaaatc gcagagctga agctcgccag caagatggcg
     2101 gccaaggtgg acagcgccct tgtccaggac ggctaccggc tctaccacca cgtcctcatt
     2161 ttcaccgaaa agggttgctg ggcggtggtc cagcagggga tgaacgacga ttacgcgagg
     2221 cgctaccact ggctctccga ccgggtggct agcttcgtcg aggagcccca ctccgccatc
     2281 tgcgcccagg aaaggctcga cgccgttttg gacctgacaa gccaagaaag cagagagaac
     2341 cgggacgtca ccctcgacct ggtgagggac gggccgtctc actttttgag gtataccggc
     2401 cagaggacca tcttcgactt cttcggccct ttgcatcctt cagatccctc ggcccggccg
     2461 atccagccgc tccggtcggc ggcgcagcag cagccggccc tgaccctgcc gcgccgccac
     2521 caggtcctgc ccgccgacct cgggaaggac gggatcaagg cgctggagct ggcatacgag
     2581 atccagcccg agagctacga ggagctgatc tccctgcggg ggatggggcc gaaaagggtc
     2641 cgggctctcg ccctcatctc cgagttgatc tacggtgccg agacgagctg gaaggacccg
     2701 gcgaggtaca gcttcgccca cgggggcaaa gacggccacc cctaccccgt cgaccgggag
     2761 accttcgacc actcgatatc gaccctcaaa gaggcggttg agaacgcgaa gctcgacagg
     2821 agggagaagt acgacgccat cagacggctg aatcggtaca tcggcgaaga tccctgaagg
     2881 ccaaagatat gatctatatc aagagagcta aaccaaaatc ggaggaagat tcgtgctgga
     2941 tctgctgaga aagaggagga gcgtgaggaa gtacagggac gaggatatag agcccgaggt
     3001 cgtggagctg ttgaaggagg cggccctcag gtcgcccacg tcgaggaact tcaggccctg
     3061 gaggttcgtc ttcgtcgaag atcgaggaaa gcttgcggct ctgtcgaggg cgaaggagag
     3121 cgggtcgtcg tttctcgcgg gtgcgaggct cggggtcgtc gtctgcgccg acgagggaga
     3181 gtcggacgtc tggatcgagg actgctccat cgcatcgatc atcctgcagc tggcgggtca
     3241 gagcctcggc ctcggatcct gctggatcca gataaggctc cggatgcaca ccgattcagt
     3301 gaccagcgag gagtacgtcc gttcaatcct tcgccttccc gaaaatatca gggttgagtc
     3361 gatgatcgcc ttcggctacc ccaacgagga gagggggccg gttccggcgg aggagctgga
     3421 ggctgagaag atcctatcgg cctgaggagg accggcggcc ttgacctcaa gaggaggagg
     3481 gcggaccggc gatggggacg gagagacggg cggctctgaa gaggctcaaa cggcgaaatc
     3541 tggggatttg ccccagaggc ctccggttgg gcctccctgg gtggccgatc tcccacccat
     3601 cgaatccaag cccatcgcta atgtcagatc aattatcgtc agggtttcga tcagggaatt
     3661 tgccgatgac catctccatc ccttcaggat aatttcaaag catttaagta taattacata
     3721 ctacattaaa gatggaggcg tcttcgatga agagagccct tctcatattg gccgccctgg
     3781 cggccctcgg catattctgt ggaggggtcg ccgacgtggc ttccggcgac gagaactact
     3841 acgaccacag cgaagagcat agcagcgggc cgggcgagcc ccccgatgac ggcatggcca
     3901 gagaggagcc gaggacgagg ttcaaggatc gttgaaggat gatggcgacg gcgctggagc
     3961 cgcaccatac tttttttcta gaccctttct caacccggtg ccccagaaac ctatggtcca
     4021 gcggaccgag ggaccggagc tccgagatta tcgtgaagcc gtcgaccctc aatccgccac
     4081 caaaacgggg atactccccc tccgccatca gacctccatg aagatcggaa agaagccgaa
     4141 gcccgagtcc cccgaggaga tggccctcgt ccatcacgcc ctggagagcc cgatccggag
     4201 gaggatgatc atcctgatgg tggaggggtg cctctcggtc gaggggatct ccggggccat
     4261 cggccctaag atgctcggct accacctcca tcggctggag ctggcggggc tgattgaggt
     4321 ctccgacggc accatcgccc tgacggaggc gggggaggcc tacggcgccc tggtcaaggc
     4381 ccaggcagag aggggcgggg cggaatgatg cccagaacca tcgttcatcc tcgatcctga
     4441 gaggttatgg ggtggcgaga ggggagatcc gcccttccga agggcctcgg cccatgatca
     4501 aggaaggata aggggcgttg gaaaaatggg ggacggcagc ggccgccgtt ggcggcccga
     4561 tctcagattt gagccgtccg tttcaaggct caggccctcg ccttcagctt catctcgatc
     4621 tcgagctcgc cctcctccag ctcaacctca agctcgatgg gaggcgcggc atttaccgag
     4681 atggagacct cgccgtaggc gatgctgacc gcccctctgg cctcgatctc ctcggcgagg
     4741 gccttcagaa aggccgcggc ctccgagctg gtcatggaga acttctgctt gaactgatca
     4801 aaacccttct cagcgctgaa cttcatcgag ctcttccccg gctcacctga tagcttaacc
     4861 atgattatcc tccctatcca tattctcctt cctccattaa aggcttctcg ccgcccccga
     4921 ccgggagagg ctccttctcg ggggtggacg catgaagaat ggaggatggc ggcggcgggg
     4981 cgccccctgg ccggcctaca gtccccagat tgatgggact gcggattatg cctcccagat
     5041 ctgctagttg tcagcttttc gtatcggcgt aattgttata aaccttgatc ataaaatgct
     5101 ataccggaga agaggaggtt agtgatatga gaggacctgt gctggctata ttttgcctcc
     5161 ttctggcatc ggccgccgga gggatgccgc caccggaggc gccaacgggg agggatgcga
     5221 cggagccgct ccaagaggga tcaagcctgg tgccgccgga ctggctttca tccgacgtcg
     5281 gcttcatcac cacctatggc cggttcggct atcctatcgc cgtcggcctt tatgatgaca
     5341 ggttatttca cgatgagtgg tatcctgtag cccgatcctt cctggatggc agcatctacc
     5401 ggagccgcta cggattctac agctatccca tcttcggggg gctctacgat ggtagacccg
     5461 gccaggacga atggtatccc gttgcccgat ccttcctgga tgatgccgcc ccgccgatgt
     5521 attattacgg ccccctccaa ccgactcaca tagatggcac ctggtggaag gatccgcgag
     5581 gctataggcc catatacaaa cctgagcctc gattcgttcc catcttttgg aggtgatccg
     5641 ccatcggatg cgtcggcggc acctcgaagg ccgccgaccc tttttgagtc cgaaggacca
     5701 gagagaccat cgccgatggt ggcccgcctc cttccgccct cgctccaccg gcggcccggc
     5761 ggattttaga tatcaaaaaa tatatatctt tatagctccc cttactctct gagggagccg
     5821 gcaatgaagg agagcattag cataggccag aatgttgatg accaaccgga gagcgtctca
     5881 gggggttctg aggacgattt taagggaggg cctccccagt tcctcgattc gcttctccat
     5941 cgaccgatca tggtcaggat gaaggacggt cggcccatca agggcgtcct tgtggcatac
     6001 aacaactacg agcttctgat agacctgggc cagagtaaga ggctgataat cttcaagggg
     6061 gcgatatcct cgatcgaggc cgaggagaga ccggagaaga gggctgggag gaggtgagat
     6121 aaatgaccga aggatcgacg aggtcgctgg acgacggcga cctcgagttc gtcgagatcc
     6181 tccagagcct cggcatccag cgggcgatgg cggtggtgat cacatacatc tccaaggctg
     6241 gcgaggccac atcaaaggag atcgaggagg cgacgcagct acgccagtcc gaggtgagcg
     6301 tcgccgttca tggcctccgg ggggaggact ggatcgatga gcggacggcc cagccccagg
     6361 ggaaagggcg ccctccggcg gtctactccc tcagcacccc tctcgagggg atcataaggc
     6421 ggctcgaaga ggagaagagg agggagcacg ccgaggtgat ggagaagatc cggaggctga
     6481 aggcgctggc atgattttga tgcggcggag aggagccaaa atcccagggg ggtcagaccg
     6541 gcggccgccg atgggtggat ggaggcaacc ccagacctcc gatctcctcg accgcctcca
     6601 ccaccctcgg ccatccgggg caataataga cctggtcggc gtaatcctcg atatcggtgc
     6661 cgttgatgct ccagggatgg acgaagagga tcccgcggcg atgggggtgg ctcctgacga
     6721 actccacgat gttgaggtcg aggtcgtcga tcaatacgtc ggaggggatc ttatgcttgg
     6781 ttccggtccc ggcggtgaag tagcccctca ggcacggaaa gtgggctttg agccaacgcc
     6841 tggtggcctc ctccgcatcc gggcgcctgg cggtcactac gaagacctca tggtctttca
     6901 gccgttctac cccaccctga gagccctcta tcaggggcag gttcaaggtg tactccgggt
     6961 ctgccaatag cctcccgacc tccgaccaca accttcgccc gccgaaggtc cagtctgccc
     7021 ggtcgacatc gcccttatgg tacctctcgc catactctct ctcgatcacc tccaggaccg
     7081 cacccacctg atcggcaagc accccatcga tgtccacggc aatcttcaaa tccgtccgcc
     7141 tcccgggacc tattttatgg gcttctctcc cctggcctga gccctctttc gcctctcttc
     7201 ctgcttcttc ttcctctccc tctccttctc tgtcatctcc ttatctctct tgaagagctt
     7261 gatcatcatc gacccctcgc tctatcccag gatagaggag aatattcgat ttgatcatat
     7321 taatttatta tcatcccacc tcccacccgt ccagaagatg agatctccgg gagggatacc
     7381 gggacctatg aaggtggacc gacaaagaat ttattctcaa gtccgccatg tacatccatg
     7441 agaggcatct ggccatttat cggctttctg gtttcgatca cccttgccgc cggatctccc
     7501 gcctccggcg ccctcgccga tggttcggaa gaggcgtcct acgatgccga tctccggttt
     7561 gtattcgacc gggacttcgc ccccttcacc tatgaggagg ggggctccct ccggggcttc
     7621 gagctggacc tcctccggct cctgggggag gaggtggggt tccggctgat ccccgagccg
     7681 atggtctgga cggaggcgca acgcaccttc cagcgggggg aggcggaggt gatcggcggt
     7741 ctggtcaaga ccgaggagag gctcgccgag ttcaacttca cagcccgccc ccacgggacc
     7801 ttcgacatcc tcaccttcgt ccgggatgac ggcgaggtca tatcggccga catgctagga
     7861 gggagaaagg tggcggtgca gaacgggagc atctccttcg acctcctgaa ggaccgaaac
     7921 ggcatcgagg ttctggctta cgaatccgag ggggaggccc tcctggccct caaaggggga
     7981 gaggccgacg ccttcatcgg cggaggctcc accacccggt tctggatgag gttgcatgac
     8041 gattccggga tcaggcctct cgccaccccc tgggagatgc actccctcta ctttggggtg
     8101 agagatccgg agctcctcag gtccatcgat gcggcgatgg tggagctcct ggaggacggg
     8161 aggtatgatc agctctaccg cagctggttt gtccaggagc tggacgactc ggaggtttcg
     8221 gccctggcag acgcctcccg gaaggcgagc ttctgcgcct acgcccccta ctcccgctac
     8281 cccgtcggcg ctgcggtcct cacctcctcg gggaggatct acaccggctg caacgtcgag
     8341 aacgccctct acggactcac caccagcgcc ctgaaggtgg cgatctataa ggcgatctcc
     8401 gagggggact ccgagatccg ggcggtgata aaccgtctcc ccgacgggaa ggccgcggcc
     8461 ccgacggggg aggagcggca gatcctcttc gagttcggcc gggggatcct ggtcgtcctg
     8521 ggagaggagg ggaactactc gacgaagatg atctcggaga tcttccccta ccccttcgag
     8581 atgagggatt gaggcggagg cgagccccct ctgaagtatg caggcccccg tccctacgga
     8641 gctcgaggga gtgggatggc atgggccgcc ccacctcaac cctaaccctt caggaggtct
     8701 cttcggtgga ggagctcgag gctggaggcg aaggaccggc aggtgagggg gtttgccatc
     8761 gcctcgggga ggccgagctc ccggagggtc cgccgacaga gctcccccct ctccagcgac
     8821 ggagccgacc ccgccaccct gatgacggcg gcgtggatcc tctggagatg aaggagcccc
     8881 tcgtcgatta tccggcgggc ttccgccccg gaccggggat cgtcccaggc tgatagaagg
     8941 agccggaccc cctcgatccc cctcaacctc cggaccgatt ggacggagct caagaggtcg
     9001 tcgtatatcg gcatcccccc cggcaggggg atggcgtcgg cggagaagag gaccccccct
     9061 tcggccatcc agagggagat ggagccgggg gagtggccgg gggtgtggat cacctccaga
     9121 tcccgcccgc cgccgaggtc gatccggtcc ccgtcgacga gctcctcgtc gaccccgacg
     9181 gagccggcca ccaggtcctc gaagccgggg acggggcggt cggcgagctg ctggcagacg
     9241 tcctcgatcc atcccctatc ggacgggtgg cacgccacgg agcagcccga cgcctccttt
     9301 acggcccggg ccgcccccat gtggtcgggg tgggcgtggg tcaggatgag ccgcgagatc
     9361 tcctcggggc tccggcccgc acccctcagg tggtcgaaga tgatcctctc ggaggaggcg
     9421 acgccgctat cgatgagggt tatctcctcg gcgcatatga ggtaggcgta gacgaacctc
     9481 gggacggagg cgccggtggg gtctctaatc tcaaagggga tcttcagggg gtggatcggg
     9541 gatgggatgg tcatggcttc acgaccttat cgagctctca attaaatggt ctgttgcgta
     9601 tattcgctcg ggttatggag ctgcgatgga gaaaacggga tcacttccta cccggagctt
     9661 cgtatattag ctatagtaaa gcgaaggcta ttggagggat ggattcgttc tctcactaac
     9721 tccataactg atgcatctaa cgtttggtag accgatatca acaactgcta actgtaatga
     9781 acctaatatc ctccaagtgt tccaagccta aattctccct catatgctta ataagctgag
     9841 gcttgtattt tttaatcata tcggtgttgt ttccttgata aagttctacc tcccatatcg
     9901 aatagtattt tccatttcgg cggtcactca aatgtatatc acaaaccgcc tgacaataat
     9961 aaaagaaatt aataattctg attaatgtat gtgccggatg ttcctttcta aatctgggaa
    10021 aatagatggg ttctctgaag tggaatatgc tgggcgaagt atgggatttg aaattgatgg
    10081 cgttcgtccc ctttctcatg ttgcattcct tgcataaggt ttgggagttc tccacgtttg
    10141 cttttccccc ctgaaacaca ggtattatat gatcaatctc taatataact ccccgtccag
    10201 tcctaaaaca acactggcat gtatacccgt ctctcttctt tatttgacct ctttccgcct
    10261 cagttagcac aatatcattt ccattttccc catcaccatt attctcggta agaaccttac
    10321 cgcccatccc ctcgaggacg attctcattt tagctaattc gaaagccatc acgaattttc
    10381 taaaatcttt gtagaaaaat ttccatagac tgccatcttt atcaaatagg gacatcaatt
    10441 caagatagcg ttgattttcg gttctagata ttaaatcttt tacaagccta tctatatcat
    10501 aattctctct ttcttcgaag gagtgatact caggtttcgt tttgttcttt gctatgtgtc
    10561 tcgatattcg tactaaatct ctaagaagat ttcctgtcaa ttggtctttt tgtaagtcaa
    10621 aatattcgtc tctccattca gtaattttgg gctcaattga tcgttcatct aatctctcat
    10681 cagaccaaga gtccgggatt tcattgaatt tctcatcgat aaatctttca aacttttctt
    10741 ttgtatggtc atatatagtt atatattcta taaagtgttg catctcctct tgagatgacg
    10801 aattatcgtt ttccaagctc actgtaatat ctgtcatgta ccatccaaca gggatgtaac
    10861 ttaaataggg catgtcagga actgccccac tctcgatttg cctgcttaat tgctccacca
    10921 ggtgaattga cacaatctca taaggtctat aacctcggac tacatgagca tcttctaagc
    10981 cttctcctgg atgtgcccat acatcaatta gtcctttcca attatcaata aagaggacta
    11041 cattagcaga gtcctttcca gaacctcccc ctgccttttc cccccttaga gcacgaccga
    11101 ccatctgggt cataaggatc gagcttgtag tctgcctggt cagcattacg gttttgacat
    11161 cgggaatatc cactccttca gtcaacatac ggatattaac aagaacgcct tcttttgact
    11221 tcttgaaatc gtttatgatt ttctcgttgt tgaatagatc tgccttaacg gaccgcaggt
    11281 tcgatttatc atggatcttg gtaaataccg cgtcagcttt aatgcctctc tctaccaatt
    11341 tctctttaat atatacacac tgcggccatc tatcggcgaa aacaatagtc ttaccatatt
    11401 gattcctatt tttaatataa tcatcgacta tataattatt cctcgcggaa tcatctgcta
    11461 acatttggat aattccctct ggtaagtctc tatgatccct tactaatcta ttgtaaagct
    11521 catcattaac taccaagtct ttacctgttg gcttttcgat ataatgcggt atcgcgagta
    11581 ttttttgatg gattaactcg ttctgattag cttcatagat tatttctcga tcgaaaatct
    11641 tgaatagcca accaccgatt tgtttatcgt tatgggttgg tgtagcggtt aagcctaata
    11701 tataaaagtt aggaactagg tctttcatgc cattccagag atttcggaaa ccgtaagcag
    11761 gagcatggtg ggcctcatca aggacgacaa agagaccaga ttcagcacat gccttaataa
    11821 acttcttaaa ttcggtctct atgacatttc cgtttatatc tctagactcc gtgttgaaat
    11881 tcaaaattgc tgtttgggtc gttataatta atacgtcgtc agtagagcat attgttgaag
    11941 acttagaatg ctcagaactg cttgaaacga ctctgatatt tattctatcc ctatttatta
    12001 tctcaagagc gttatcagag aatgaagcaa acgcttggtt aagaagatac gaggattggg
    12061 cgaaccatat caccttgata ttttttggca atacgttatg gcagatccaa ttaactgccg
    12121 tgaatgtttt gccgccacca gttggaagaa cgagaagtcc tcccttatat gaattgatgg
    12181 gaaaagtgaa attcttagac aattcctcaa aagcttttgt ttgatgggga aaaggattat
    12241 actggatata tcccggagta gtctcacgca tatctctcaa atcgagattt ataaagttca
    12301 ttgacatccc tctgatatag caacccattt tcattgattt cagtatatag atcttttcct
    12361 cggtcataaa taatggattt caaaatggtt gggttctctg gaaatgtcca tattggtctg
    12421 cagggagtat accttactgc tttcagatcg gcctcgagta ctgaggctca tcctggaggt
    12481 cgtctaaagg gattccgggg cggatctcaa aaaatagccg acaaattaat taaattatag
    12541 atataaaaaa ttatagaatt tacctacgtc gcttccgccc ctttttgagg cgggagacta
    12601 atttgccgag gcctatgagg gccagggcct tgaagagctt cttggccaac cccatcggag
    12661 atcaccgccc ttacctcagg cttccacggc gaaaaatgtt tcggtttggc ccccggccgg
    12721 gggggggcct tgccgtggac ctatcgccct ctagggcctc aagggccgga ccggccccac
    12781 ccacccatca tcccccggaa ggcccccagg tccccgggga cgaggttgag gcggtgggcc
    12841 acctgctggc tgaaggcgag gccgcccgaa ggggccgggc ttccgccctg ggccggagcc
    12901 ccgagcctgc ggccgtcctc gcctccgacg aaggccatgc tggcgcctcc ggcggggtcg
    12961 atcgaaaggg ccaccccata ccgggcgtcc tcctggaagg tcatcctggg cccccgaatg
    13021 gcgtaatcct ccgggcagat atcgctcatc atcttcggcc cccccacccc gtcgaggccg
    13081 ccgctgcagc cgcaggccgc cgccccgggg gcgagggcca tcaccgccag gaccaacagt
    13141 actatcgatc tcaattcatc tcaactccct ttacgtcaaa gatccggatc atccagatct
    13201 tgtatgctat ctcgtcagca caagctaaat atccatcgga tctcggcgac caaggaagcc
    13261 ccatcttgaa gttcatctaa agatcgtcca gaagatctca tagatccctc gatcgatcac
    13321 tcaaacctct tctgaaggat cttcgccgcc ggatagatga agatcaaaag aaagccgacg
    13381 aggacgaggg aggcggtctg cgggcttgat gatccggaaa agaaggcggt tcggagccct
    13441 tcgacggtgt aggtgagggg gaggacccgg ctcaagagct gaagcccctc gggcatcgct
    13501 gatatcggat agacgacgcc gcagaggaag accatcagga acctggggag gttcaagaag
    13561 gtctgggcct cgaagacctc cttgaagccg acgctgaaga gggcccccat cgacgaatag
    13621 accaaaaggg agatcaaaac caccgggatc agggccgcaa gatcgggccg gaggcggagg
    13681 ccgacgacgg tgctcaacgt caccaccgag gtcatcaaca gcccgaagag aaagccgccc
    13741 aggaccttcc ccaggaggac ggcgtcggtc ctcaccaggg ccaggaggag gcgctccagg
    13801 gatccgagcc taagctcgaa gtttatcacc accgcctccg ctgccgtcgt gctgaagagg
    13861 atcgtcatgg cgatcagccc cgggacgaga tcttcgaaat ccccggggct cctcagaaag
    13921 aaggcgaggg tccaggccag ggggaagacg atcccccagc ttatcgccgg gggcttgagg
    13981 tagtaggtcc tcatgtcctt tgccgctatc agccatatgc agcggaggtc gtcttggatc
    14041 ataccccatt cccccccttc tcctttgcca tcaccgtcgg gctgaggccg gttatcctga
    14101 cgaaagcgtc ctcgaggctc ggagtcaagc tgtttatcgc ccggagcccc aggttgtggc
    14161 ctcgcgagaa ctcgaagacc tcctccagga cccgggaggg tctcctccgg agatcctgac
    14221 ccggatccac tccaggaggg ccaccccaga gccggggagc cgctccgaga ggcggggcca
    14281 aaggtcaaag gcggatctgc cgaaggtgac ctcgatgacg ggctcctcgt cagtggacct
    14341 cttcagaccc tcaggcgtat cgaccccgac gaccctccca ccgaccagga tggcgatccg
    14401 atcgcagaga tggtccgcct cctccagata gtgggtggtg aggactatcg tcagccccct
    14461 atcgtggaga tccgagacca ggcttcggag ggagcgggcc gccaccacgt ccaaccccac
    14521 cgtcggctcg tcgaggaata ggaggtgggg atcgtggacg agggctgcgg cgatggtgag
    14581 ggcccgcttc atcccccgag agaaggtccc gaacctgtcg tcgccccggc cgctgaggcc
    14641 gaaggccctg aggagctcct ccgccctatc ttcccgatcc gccttgggga ccccatagag
    14701 accagccatg aagaggaggt tctctcttgc ggtcagatct tcgtaaaggt ttgaggtgtc
    14761 ggggacgacc ccgatccgcc ttttggcctc gacgatcccc gaggagaggt cgtatccgag
    14821 gactctggcc gtccccgccg tcggccggga gaggccgacg agcatcctga tggtnngccg
    14881 ttggggccga ggaggccgaa gacctccccc tcctctacat cgaagctgat gccatcgacg
    14941 gcgaccagat cgccgaaccg cttggtcagc ccctggactt caatcgatct caaacctcac
    15001 ccccaaaacc caacgggccc tctgccaacg cccccccaaa aaaggcaggg gtctccagcc
    15061 tcaatccccg gtcagctctc tcagcttcgc ctcaaccctt tccagctccc tcctgaggga
    15121 acccctgtac tcctccaggg tcgatatcat ctcctcccgg ctgatggggg ggtggactcc
    15181 acagcaacag cctccgccct ctccagaacg accgagaccg tggtggccgg cctggtcgtg
    15241 gccgtggccc tggcatccgc agtcctcatc tctgccggca cccctcccca gggggggtgc
    15301 gcgataacct gcatcatctc cgcagcgcat atcatttcac ctcgaaatct tccagaagat
    15361 ctatgacaat tctccgggtc ttcagcctcc agaccttctc cggagttccg gcttttgact
    15421 ctctataaga ggccacctcg acgatccctg cccccttgag ggcggagagg tgatagtaaa
    15481 gcccggccag ggatatctcc tcccccagcc ccagctcttc aaagatctcc ctcgtgctcc
    15541 tctcctccgc gccgatgcac tggatgatcc tccacctcgt ggggcagtgg agagcgcgga
    15601 tgcacctctg aacctctctc agggcctggc tttgtccgtg gcacataatg atcgccttga
    15661 atattctagc gattggcttg agaacttaag cgcacgatat taaagctttc ggccattgcc
    15721 acagaaaagg gagccgtatc cctcaggccc gacgggcccg agggccccga ccttgagggt
    15781 ggagggagag ggaactcggt cccaaaggca gccgcttccc ccttcggcct ccctatcgat
    15841 ccttcgggcc gccgacggga ggctcagccg gagaccgccg tcggataggg cacgatcagg
    15901 atggcgggcc cctcccccgt ctccctcgac ctgaagctta ccctgtagtc gacatccgcc
    15961 accgccatca cggcgaagga taacctctcg ccttccgccg ccttgaggag ctcttttacg
    16021 tcgaagacga cgatccggtc ctggccgaag ctcatcccga tccggcggag gtcgatctca
    16081 gagctatcct ccaggatgga gatcatcgag aggaggttca acgccagggc tgggaagctc
    16141 gagccttcgg tccactcgga ggttatggga acgaggatca atcccgaatt ctcgtcgccc
    16201 tccttatcga tcgcctccgc cttcagaacg aggacggcga cgtcgtcctc ctccaccacc
    16261 aggtcggata tgttgaactg gatgatggcc atcgctggaa actcttccgc ctcgtaaccg
    16321 cggcccttca ggtattcgca gaccagatgg tcgtcgttgt agaccatctc ctccatcaag
    16381 tccacgtaaa cgtcctcggc tgccagatag accgcccctg agcagccata tgccgctggg
    16441 tggccgggga tctcgcctgc cggcgtttcg ggcgagagca cctcagaggc ggtcttgttc
    16501 ccatcatcaa cggcgatcag acccacttcg ccggtatctt cggccccctc cccaaagatc
    16561 tcagcacctc cggagggggc ggtctcgttc aaagacgatg tttcgttaaa agatccacct
    16621 tcttcaacct ctccatcaag atcatccagg acaggcctca ccccagtatc gccaccctgc
    16681 gccgaagccg attggacggc aagggataag gagagagcga tcaggatgca gaagattcct
    16741 ctatcgaaca tacaaaattc atctcccagg attgtaaatt tgagcttagc atgaatatat
    16801 tcttttccat agatacgtat gaaagaagga cgccgatacc gccgtggccc aggccgacgg
    16861 atctttctga gggccgcgac aaaaattgat cctccccccg gaccgatcct gcgggctcgg
    16921 ccatccgagg gaaatacaag taaattttat tatgggcaaa atattatgat caaatatttt
    16981 tgctataatt caatatgaat tgattaaatc cctgaccgcg cgtcttcgaa tgccattcca
    17041 ggatggaaaa agagcccatt cccaaccgat tttgcggacc ggacggctag ccctccaccc
    17101 cataaaagag gagacaggaa gggcctggcg acgaggtatt tcggtcaaaa ggtcattatt
    17161 ataagatata atgctaactt tttcctgatt ttaaggataa atcagactta gaaaagcata
    17221 tatagcaata aatgtcatgt acgcgctgtt taatattaag atatcggagg acgggatgaa
    17281 aatgagcggt gtagggacga ttatgaggat ctggccgttg cttctggtgt tgctcgcgat
    17341 ctctggcccc gcgtgggcgg ccaataaccc ggatggatat tacgtgaaga acgagcaagt
    17401 cttcgaacag gatgtcgagg gacaaggtta cgtcatggtc taccagaggg tgaacaccga
    17461 gtccctttac ctcaagaact accttcacgg gagcggcacc atggatggtg caacactgat
    17521 ttactccaac caatctagcg taagtggcga ttataatgat cagaataatt gggtatatgg
    17581 agtgtggaat aagacaaaat gcagttacga ttgtgatccc atgaaaaact ggtgcgattg
    17641 gaagaagccc catgccgaaa atatcacttt tctcgagcag aatgagatga cctacgcccc
    17701 ggtggcgatg gcctacggca cgggatacta cgctgaaaat ccgataatct tcaactcaaa
    17761 gctgaaggag aggaccgagg ccaagaacta caatagcgca tgcgaaagtc cggctgtatc
    17821 gatggtccac cagattgaat atgcctcggc attcaataag gacattggcg taacactcca
    17881 gaacatcgac aaatggcctc ccaccggtta cagcttaaag cccacgaaag gctccagcct
    17941 caccaaaatg aagatcgagg aggaggttac tgaggggacg gtgcacatcg gccagcttgt
    18001 gacgaggccg ttgcctagag ataaaaaaac gaataaaccg acgaccagat cattgtatgg
    18061 tttgaaaaat cccctcgtcg agatcgatga gaactacatc ggcaccttca agatcaccaa
    18121 gaacatggag atctgcacca gctgcaccgg attaaagccg agggacgact ggctctcctg
    18181 ctgcataggc ggctacggct ccatgcagga ctccgacaag ctctggggcg aggaggagat
    18241 cttcgactgc acctgccgcg agaaggcctg gggccaggcc tggaacgaca cctccaaggc
    18301 ccaatactgt gaagactgca aatgagaata tcacgatggt atttgagagg cgcttgccgc
    18361 cttagggcca agcgccgctc cctccaactt ttttagtcga tttatcagtt tcagccgatc
    18421 gggagaaagc cgatctcatc caccagcgac tgcccctcct cccccagaag gaagtcgatg
    18481 aaggcctctt caccagcgtc cggctcgttc caggtcatga cgtagagggg gcgggagaga
    18541 ggatagtcat cgcccctgat gctctcggcc gagggggcga ccccatcgta ggcgatcgcc
    18601 cggaggggac cgtcctggac gtagttgaac cccagatatc cgatcgcctt atcgctcttg
    18661 gtta
//
LOCUS       DASM01000179           11669 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_9451, whole genome shotgun sequence.
ACCESSION   DASM01000179 DASM01000000
VERSION     DASM01000179.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11669)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_9451"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
     assembly_gap    9444..9463
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    11444..11462
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2297 a         3537 c         3646 g         2145 t
ORIGIN      
        1 caagggccgc atcatcacca ggaactgcat gggccccata aggatggggg acgtgctgat
       61 gctgctcgag accgcaagag aggccaagaa gcttaccacc aggtgaggcc tcatggaaga
      121 gagaaagtgc tccttctgca accggaagat agaggccggc acagggaaga tctacgccaa
      181 aaaagacggg acggtttact acttctgctc gtccaaatgc gagaagaaca tgatcggcct
      241 ggggagagtc ccgagaaggg tcgagtgggc gatcaaaggg tggtagggct tggagcagac
      301 cttcgttatg atcaagcctg acggcgtcca gcggggcctc gtcggcagga tcgttcagag
      361 gatcgaggcg aaagggctga agatcgtggc gatgaagatg aggactctgc cccaggacgt
      421 cgcccgggag cattacgccg agcactccca gaagcccttc ttcggagacc tggtgagctt
      481 tatcacctcg ggcccctcgg tcccgatggt ggtggagggg aaagacgtca tctccgagat
      541 gcgaaagatg aacggtgcca caaacccgac gaaagcccag cccggcacca tccggggcga
      601 cttcggcctg gacatgggga ggaacgtcat ccacggctcc gactccccgg agtcggcgaa
      661 gagggagatc gccctccact tcggcgaggc ggagctctcc agctacacca ggatcgacga
      721 ggcctggctc tacgagtgag cttctgtccc tccggcagct cccagatctg ccaggagggc
      781 ggcctcgccg ctataggaac tgacggggtc cggatctgat ccagcatgaa gcagaaatcg
      841 aggaaggcga agaaggggaa gggnnngacc acggcaagac cacccttctc gacaagatca
      901 gaggcaccac catcgctgac ggcgaggccg gggccataac ccagcacata ggggcgacgg
      961 agattcccct ggccgtcatc accgaggtct gcggcccgct attcaggggc gacttccaga
     1021 tcccaggcct cctcttcatc gacacgccgg gccaccactc cttcacctcc cttcggagca
     1081 ggggcggctc cctcgccgac ctcgccgtcc tcatcgtcga cgtaaacgag gggttccagc
     1141 cccagactat cgagtcgata aacatcctga agcgttaccg cacccccttc gtggtggcgg
     1201 ccaacaagat cgatcggatc tcgggatggc tcccggtgga gggggcgcct ctcgccgcga
     1261 ccctcaaggc ccagagcgag agggttcggg gagatctgga caaccggatc tacgagctgg
     1321 tcggcgagct ctacaagcag gggttcaact cggaccggta cgatcggatc agcgacttca
     1381 ccaagaacat cggcgtcgtc cccatcagct ccatctccgg ggagggggtc tcggacctgc
     1441 tgatggtcct cctcggcctg gcccagaagt ttttgaagga gaacctgaag gtcagggcca
     1501 gcggccctgg ggtcgggacg atcctggagg tgaaggagga gcggggcctc ggcaccaccc
     1561 tcgacgtcat cctctacgac ggcgagtttc ggtccgggga taccatcgtc gtggggaccc
     1621 tgaaggatcc gatcgtcacc aagatcaggg ccctcctcaa gccccgcccc ctctcggaga
     1681 tcaggagcga ggagcggttt cttcccgtaa agcacgtggc tgccgccagc ggcgtcaagg
     1741 tctcggctcc caagatcgag agcgccctcg ctgggtcgac catcagggtg gtgagcgagg
     1801 acgaagatgt cgatgccgta gcccaggaga taaggtcgga gctggagacg gcgaggatcg
     1861 acaccgagaa cgtcggcgtc atcatcaaga ccgataccat cggctccctg gaggcgctgg
     1921 tgggggagct ggaatcgaag gcgatcccga tccacgccgc cgacgtcggc cccatcaccc
     1981 ggagggacgt gatccgggcc gccgccatcc aggaccccct ctactcggcg gtcctggggt
     2041 tcaacgtcaa gatcctcccc gacgccctga cggaggtcca gaagagcgac gtgccgatct
     2101 tcctctccga ggtcatctac aacctcctcg agaactacga tgactgggtt gaggaccaga
     2161 agatgaggat ggagcaggag aggctccagg ctgtgatcag gcccgggatg atcaggatca
     2221 tccccgacta cgtcttccgg cagtccaagc ccgccgtcgt cggcgtccag gtggtgggcg
     2281 gccagatcac caacggcgtc accctcatga gggaggacgg ggcggtgatc gggacggtga
     2341 agggggtcca ggcgagcggg gagaacgtcg gatctgccag tgtcgggaag gaggtggcgg
     2401 tctccatcga cggccccacc gtcggccgcc agatccacga gggggagatc ctctacgtca
     2461 acatccccga gaagcacgtc aagatcatcg aagcggagct gaagcagagg ttctccgagg
     2521 acgagctgga ggctctggag aagtttttgg agatcaagag ggcgaaagac cccttctggg
     2581 gtcgatgact cccaaaagat cgagatctgg acgcccctcg tccccggcta atagagccgc
     2641 ctttttccgt tcgatctatc ctctctcttc aggacccctc ctcctctcgg agggggtttg
     2701 tcccggtcgg gaagggctgg atcggctaaa ctgagactag atcctgtttt tttcgatttt
     2761 tttggcgttt tggcccctct tccgatctta aatcttcata attattgtga actcgtctca
     2821 aacagatata aatcgccggg gaggagtctt gaaggaggag gcagaaaaga gatggtaact
     2881 tcgtccagca aggacaaggt attcgaagcg atcgaatcga agggcgttaa gttcgtacga
     2941 ttgtggttca cagacatcct cggcctgccg aagagctttg ccatttcccc gtcggagctg
     3001 gagggggcct ttgaggaggg gatgggcttc gatggctcct ctatccaggg tttcgcccgg
     3061 atcgaggaga gcgacatggt ggcgaagcca gaccccgaga ccttcacgat cctcccctgg
     3121 agggcgaagg aggggcagaa cgtcggccgg atgttctgcg acatcctgga gccgagcggc
     3181 cacccctacg ccggagaccc gaggtacgcc ctcaagagga acctcgccgc cctgaaggag
     3241 atgggctacg actactacgt cggcccggag ctggagtact tctacttcaa agacgataag
     3301 gggacggaga tcctggacaa gggcggctac ttcgacgcca tgacgacgat ggacgccgcg
     3361 agcaacctcc ggcgagacac gatcctgacc ctggagtcca tggggatcaa cgtcgagtac
     3421 agccaccacg aggtggcccc atcccagcac gagatcgacc tccggtatca ggaggcgttg
     3481 aagatggcgg acatcgtcat gacctatcgg atcgtcgtca aggagatcgc ccagcagcac
     3541 ggctactacg ccacctttat gccgaagccg atcttcgggg agaatgggac ggggatgcac
     3601 gtccaccagt cgatcttcaa gggcaagagg aacgccttct tcgaccccgg ggacgggtat
     3661 cacctctcgg acttcgggaa gcactacata gccggcctcc tgaagaacgc cccggtgatc
     3721 accgccgtca caaaccagtg ggtgaactcc tacaagaggc tcgtccccgg atacgaggcc
     3781 cccgtctaca tatcctgggc gaggaggaac aggtcggccc tcgtccggat gccgatgtac
     3841 aagcccggaa aggagaacgc taccagggtc gagtatcgga gccccgatcc gggggcaaac
     3901 ccgtacctcg ccttctcggt gatgctggcg gcagggatgg acggcgtcaa gaacaagtac
     3961 gaactccccg agccggtgga gcgggacatc ttcaagatga ccgaggcgga gaaggagaag
     4021 gcggggatca ggtccctccc gggaagcctc atcgaggctc tgatgctgac ggagaagagc
     4081 gaccttataa gagaggccct cggcgaccac atcttcacca acttcatcgc ctcgaagaag
     4141 gtcgagtggg acgagttcag gaccgacgtc agccggtggg agatcgagaa gtatctgccg
     4201 ctcttgtgag cccctcccag caggctcccc cgaagggccg cttctggccc ctcccggggg
     4261 agagccgatc ggattcggca tgaattgaga cgatccgggc gcggccccac ccttccatca
     4321 caacgagagg tgatatccac cctttggggc gcgcctcgat cgaaccctcg ccttcatgcc
     4381 gtcgatcaac tttttttgta gctcatctcc ggagggcgga ggtttccgcc ccaggggtgt
     4441 tgtggttcct ctcaccgatg atgcttcggc ttcaaagctc atcataatat gaattcaggg
     4501 ttaagaattc ttcatattga ttatgataag agcatatatt accacaaata cgcttataaa
     4561 atgagcagct ttctccagat catggatctg cccatgcgaa gactcttggt ctcatcggcg
     4621 cttttggggc tcatcgggac cgcctcggcg gagtcggggg tgaaccccgc cgacaacgcc
     4681 tgggtcctct tcagcgccgc cctcgtcctg ctcatgatcc ccggggtggg gctcttctac
     4741 ggggggatgg taagaaagaa gaacgccatc tcgacgatga tattcagctt tgcgatcatg
     4801 gggacgataa gcctccagtg gatcctcctc ggatacaccc tcgccttcgg ccccgacttc
     4861 ggaggcgtca tcggcggcct cgaccacctg ggcctccggg gagtcgggat ggaggcgatg
     4921 gagggacaat ccatacccgc cctcaccttc atgatattcc aggcgatgtt cgccatcatc
     4981 accgtctccc tgatcacggg aagctacgtc gagaggatca agttcagctc gttcctcgtc
     5041 ttctccctcg cctgggcgac cctcgtctac gaccccatag cccactgggt ctggggtggc
     5101 ggtttcctct cagagatggg ggtcctcgac ttcgccggag ggatcgtcgt ccacatcagc
     5161 gccggagtct cggccctggc gatagccatg gtcatcgggg cgaggagggg cttcggcagg
     5221 gaccccatgg agccccacaa catccccacg acggttctgg gggcctccct cctctggttt
     5281 gggtggtttg ggttcaacgc cggatctgcc ctcgccgctg acgggatcgc cgccaacgcc
     5341 ttcgtcgcca ccaacacctc ggcggccgca gcggccacca cctggatgct cctctcgtgg
     5401 cgggaccggg cccccagcgc cctggggatt gtgacggggg cggtggcggg gcttgcggcc
     5461 atcacccctg ccgccggttt tgtgaccccc atcgcggcgg tgccgatagg cgtgggcgcc
     5521 gccatcgtcg gctactatgc gatcctcttc atccggggcg gcaagaaggt ggacgactcc
     5581 ctggacgtcc tcgcctgcca cgggatcggg tcgacctggg gggtcgtcgc caccgggatc
     5641 ttcgcctcca tcggggcgac gggcctcctc gcgggaaacc cgtcgcagct tgcggtccag
     5701 gccgcggccg cagccttcgt ctggatctac tccttcgtcg tcaccttcgg gatcgcaaag
     5761 gtggtggatg cggccatggg cctaagggtt gggaccgacg aggaggcggt gggcctcgac
     5821 atcagccagc acggcgaggg ggcttacgtc tgaaggccgg taagacggtg agtttgaggt
     5881 gtggagttcg aggcggatcg aggaggattc tcatatgaag aaggtcgagg cgataataag
     5941 gcccgagagg ctgagcgcag tgagggaggt cctggacaag aacggctttc ccgggatgtc
     6001 ggcgacggag atcaaggggc gcggccgcca gaagggggta tccctccagt ggcgggccgg
     6061 agactacaag gtggagttcc tccccaaggt gaagctggag gtggtggtgg aggacgggga
     6121 cgccgaccgg ctcatcgacc tgatctgcga ggcggcccac accggcgagg cgggggacgg
     6181 caagatcttc gtctacccgg tggaggaggt cgtcagggtg aggacgaggg agagggggtc
     6241 gggggcggtg tagttaggct ccggtccttt tccctctctg cccggtcgtt cccagccctg
     6301 cggagtgggt ggcggcggga cctctccggc ctccaaaaag ccgggctgga ggtcaaaagg
     6361 ctggggccgt cgtgagattt ccgccggagt ccgaccgtct cctcttttcc cccgagatct
     6421 gccctttcag ctaagtgtat cttatgtcgt cgacgtagat cgtcgcccct tcggggttat
     6481 cccctccatc cagccggagg ctgaagagcc cgctgatgga ggagaggtct cttcccccca
     6541 gatcgatggc aaactccctc cagccgtccg tcagagtcac cttcatatcg tcggaggcgg
     6601 agtcggcgac cccatttctg tccggctcgt acccgtagcc gaaggtcacg acctcgccgc
     6661 cccggccgcc ccgggccgcg aaggagaggg tctgagagcc cgtgaggtcg aggccgatgc
     6721 cggagcctcg cctccagtcg ccggtggcct ggacgaagac gccggcccac gcctcggcgg
     6781 agctgtcgta agtcgccctg gcgcagatat ctccgctatg ggggtcctcg tcggagaagg
     6841 ggtccaccgt cagggagtcg gttccgccat cgctgcccat gagcccggac cagggatatg
     6901 tcagggggat ctcatggtat actacggtct ctgcggatgc cggaagaagg ccggccagca
     6961 gacaaagggt caaaaccgat ataatggtct tcatatcaaa ttctctccat gctattttat
     7021 cctgattttt ggccttctgg caaagctctt ctctcagctt ccctcggccg tctcgttatc
     7081 cccgatctcg gtggagttgt cgggactttc ggcatcatct gacgtattat ggccggaacc
     7141 ctcccttgat ttctcatccg gatctttcgt tttgatttca gccagctcga gggtctcttt
     7201 cgcatcgccc gtatcgccga tcagatattt gtggtggtga tcccgaactt caaccctgac
     7261 cctttttgtc ccagcgtcgc tcccagatct ctcccagtaa caatcatctt ccgtcgacca
     7321 gttgcagata atatcccagc tgttgctcag attattaaac tgcgagaact tgcactcgat
     7381 ttcgtcgccg tcggggtcgg ttgcctttgc tttaagcaac accttcgagc catattctcg
     7441 tccagcttcg aaggtttcga tctcaggagg tctgttctct ggagctatta ggtattctct
     7501 gtgggtggtg gtgttcaggt ctttcttatc gtagacgatg acgtcgatgt agttggggcc
     7561 gctatccatc ggctttgtaa accagctcca agaattattg tattccggtc ccttgactgt
     7621 aatccactta tcgttggtgg cagggccctt ccgacgaaac tcgtatctga ggtcgttgtc
     7681 gtcttcagga tcaaaagcat gaactgtgat gaagatccta cttcctacag cgtattcgcc
     7741 tgcgtctggt tgaatctcct ttattcttgg gggtttgttc gccatgtaaa ggccagaaga
     7801 gcccgcgaca atcaaaatta atcctatggc tgccagggcc tgggcctgcc tccccgtcag
     7861 agcgagggac tgcttgatga ccgctgatat caagggcggg cagatcgcgg caatcaccag
     7921 gatcaggcca agaataatag caaatttata tgctagtggg gtggactgta cgatgctcac
     7981 gacggattcc agcattccta ttcatctccg acttgtttcg ataatataag ccatccagac
     8041 cgcatccatg ctgtcaaaga aagctcatac taaaaaaaca ttttggaatg atgggcggca
     8101 gaacatgcgc ccgcctttgc tggagtctcc gccggaggcg gcgcgagggc cgtcggtgga
     8161 ggtcatcata ccgcgcttgt tccctcttat aaaagttccc gccttcttat tcgcccccgg
     8221 cgcggcggcg ccggagcccc ggacccccgg cctctctcgg cgcgaggggg cgggggcggt
     8281 ctcctccccg acggggggac aggccggcag ctcggggggc gtggtcgggc cgcttccggg
     8341 gcggggggcg agagggcttc tttgggtcct cgggatcggc gggccggagc ggtgggtggc
     8401 ttatcccatc atcctctgga ccgtggggtt cggggggcat ctgatgggcc tcggcccttc
     8461 gggccgatcc ggctcggaat gatccggagt agatcgatct ctccgagatc tccggttgag
     8521 ccactcctca gccttcgatc aatcatctcc cgccattctt ctcatctgga gggggatcga
     8581 cccccccgcc cccttcataa ttgttatgaa cctttctcaa accttctcaa atacctataa
     8641 tacatttatt aagcctacct tcaggctgca caccatcgaa tcaagaagga atggagaaga
     8701 atcggaccga tggatggagt atcggatcat tatgccacgg aagagaagaa cccgcagaga
     8761 gctttacacc aaggagatcg cagcctgctc catcttcggg gctatgaacc gggacggcga
     8821 ccggtttaca ggagagggcg tcatgaaggc gatagccaac atgcacgtcc ggggcaacgg
     8881 cctcggcggg gggtttgccg cctacggcat ctacccggag caccgggact gctacgccct
     8941 ccacctgatg ttcacaggtc ctagccccca ggcgaagaag gacgcaaaaa aggagtttga
     9001 ggccttcctc tcccgcgggt tcgacctctt cttcgacgag gagatcccca ccgacgaggc
     9061 ggtcccggtg agagacccgc ccctgatctg gcggtacttc gtcctccccc ggatcgaccc
     9121 cgcctccgag gaggaggagg gggactacat cgtcaggtcg atcatggagg tgaacggcaa
     9181 aatcgagaac gcctacatct tcagctccgg caagaacatg ggctgcttca agggcgtcgg
     9241 ctaccccgag gagatcggcg agtacttcat gctcgaccgg acctaccggg cccacacctg
     9301 gaccgtccac gggaggtttc cgaccaacac ccaggcctgg tggggtggcg ctcacccctt
     9361 cagcctcctg gacacgacga tcgtccacaa cggcgagatc tcctcctacg gcaccaaccg
     9421 ctggtacctg gagatgtacg gctnnnnnnn nnnnnnnnnn nnnctacggg ccgctcctga
     9481 tgaacggccc cttcgccgtc atcatcggcc agaccggccg gatgatcggc ctcaccgacc
     9541 ggataaggct gcggcccctg acggccgcca ccagcgggga gatgttctac atctccagcg
     9601 aggaagcgtc tataagactg atatcgcccc acctcgaccg ggtctggacc cccaatgggg
     9661 gggagccggt cgtcggcgag ctctatagca tgagaaagga tcagccatga aatctttcgt
     9721 ctttcccgag ttccaggtca tcagggacga ggagaagtgc ggccagtgca ggggatgcga
     9781 gaggcagtgc gccttcggga cccacaccta cgacccggca gccgaccgcc tcgtctccga
     9841 cgaccgcaag tgcgccggat gccagaggtg cgtcatcttc tgccccaagg acgcgataga
     9901 ggtcaggccg tccccttcgt tctaccggcc gaacgcctcc tggtctcgcg agaagatcga
     9961 ggacctcaag aggcaggcgg agacgggcgg ggtcctcctg acggggtgcg ggagcgacaa
    10021 gcccttccgg atctactggg accacatcgt cctcaacgcc tcccaggtga ccaacccctc
    10081 cctggacccc ctgcgggagc cgatggagct tcggaccttc ctggggagga agccggatat
    10141 gatggacatc tccgccgact ccggggaggt cgagatcagg acggagctct accccaacct
    10201 ggtggtggag acccccatcc tcttctcggc gatgtcctac ggggcgatca gctacaacgc
    10261 cttcaaatcc ctggctacag cagcctgcag gtccaagacc tacttcaaca ccggcgaggg
    10321 cggcctcccc cgggagatga gggaggagtt cggccggtgc gccatcgtcc aggtcgccag
    10381 cggccgcttc gggatcgacg ccgagtacct caacatagct caggcggtgg agatcaaggt
    10441 cggccagggg gcgaagcccg ggatcggggg tcacctcccc ggcgagaagg tctccctctc
    10501 ggtcgccgag acccggatga tcccccaggg gaccgacgcc atctcccccg ccccccacca
    10561 cgacatctac tccatcgagg acctctcgac cctcatcacc gccctcaagg aggcgaccaa
    10621 ctacgagaag ccgatctccg tcaagatcgc ggcggtccac aacttcgccg ccatagcctc
    10681 gggaattgtg cgcgcaggcg ccgacatcat cgccatcgac ggcctccggg gcgggacggg
    10741 ggccgccccc aaggcgatcc gggacaacgt ggggatcccg acggagctcg ccatctcctc
    10801 cctcgaccgg aggctccggg aggaggggat caggaaccgg tgctccatca tctccgccgg
    10861 cgggatccgg tgcagctccg acgtcataaa ggcgatcgcc ctgggggccg acgccgtcta
    10921 catagggtcg gcggccctca tcgccatggg ctgtaccctc tgccagaagt gctacaccgg
    10981 aaagtgcaac tgggggatct gtacccagga cccccgcctc tccgggaggc tgaacgtgga
    11041 gatcgcctca gagaggctga gcaacctcat ctccgcctgg tcccacgaga tccgggagat
    11101 gctggggggg atggggatca anntcaacgc cctggagagc ctccggggta acagagacca
    11161 cctccgggga attgggctgt acgaatggga gctggacgtc ctggggatca agggcgcggg
    11221 agagtgatga agatggccag agcagcacta tcagaacctt tgataaattc ggatcaagcg
    11281 ggtgaagagt tgatggcagc ccagatcgac gccgccgatg taaagaccaa agagctgaac
    11341 gaccggctga gggagctcct cctctccggc gccaagaggg tccgcatcag gaacgtctgc
    11401 ggccagcggt acatcgggac ccgcctctac accccccagg ggannnnnnn nnnnnnnnnn
    11461 nnggacgggg tcggaaacac catggacgcc ggggaggtga tcgtgcgggg ccgggccgga
    11521 gacgtcctcg gcttctcgat gcgcggggga gagatctacg ttcgggacgg ctgcggctat
    11581 cgggccgccc tccacatgaa ggagtacgag gggaagaggc ccgtcctcgt cgtaggcggg
    11641 aggtcccagg acttcctcgg cgagtacat
//
LOCUS       DASM01000180           10524 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_62754, whole genome shotgun sequence.
ACCESSION   DASM01000180 DASM01000000
VERSION     DASM01000180.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10524)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_62754"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT         2152 a         3145 c         3223 g         2002 t
ORIGIN      
        1 gtcacctcca cctcccggcc cgtccagatc ttccggacca tcatctcgcc ccgggtgagg
       61 gtcccggtct tgtcggtgca gatcaccgtc gtcgagccga gggtctcgac ggcggggagg
      121 cggcggatga tggcgttctt ccccgccatg atctgggttc cgtaggctag ggtgatcgtg
      181 gtgatgaagg ggagggcggt ggggatggcg gcgacggcga gggcggcggc gatgatcagg
      241 gtgtccagaa ccggagcgcc ccggttcacc tccacggcga agatgatggc gcaggcggcg
      301 aggaccaaca gggctagctg ctttgccaga tgctggaact tgatctttat cggcggctcc
      361 tcgggcctcc tctggacgag ccccgagatc cggccgagct ctgtcctcgt ccccgtcgcc
      421 gtgacggcag ccctcccgtt gccgtagacg acgatcgtcc ccatgtagac catgtccgtc
      481 ctgtcggcga ggggggtctc ttcggccacc accgccgcgg acttatcttc cgggaccgac
      541 tcgccggtga gggacgactc gacgacctgc agcgacgtct cctcgatcac cctaccgtcg
      601 gcggggacct tgtctccggc ctccaggtat actacgtccc cggggacgag gtcccggacg
      661 gggattctcg tcatgctccc ggaccggagg acgttcgcct ccggggccac catctcccgg
      721 agcgcctcca tagcccgctc cgccctgtac tcctggaaga agccgacgag ggatatgaag
      781 aagatgatgc cgaggatggc gtaggcgttg gtggtctcgc cagcgatcca ggatacggcc
      841 gcggcgaaga tgaggacgat gatcaggtag ttctcgaact ggcggagaaa tacccggagc
      901 ttggagggct tgtagaactc ttcgagctgg tttggccctt gctcggagag cctccgggcc
      961 gcctcctcgg aggagaggcc atctcggctg gagctgagcc gttccagggt ctcgtcagcg
     1021 gtgagggtgt gccaggggag ggtcatggga tcttctctca tagtgaggtc ggtgatggat
     1081 tcttttttat gtgaagatat atcttccctt cacccgggcg ccaaatccga ccgttccgtc
     1141 ctcttttgaa gtatcttatt gctagccacc tctcgacgtc ggctgagacg atcacggctg
     1201 caggatcgcc ccgccctcgc ctccggcgcc gccctcccac ccgcgcccgg ggaccgcctg
     1261 ggatcggcac aatcttctcg cttcggctcc ccaatgcggc tttctcaggt cttttttccc
     1321 ataccatcgc ctcgatcccc agcaggttaa taagcagacc cggatacaat aacaggcgat
     1381 aatcgggact gataccatgc ggcaggtcat aatataccca ggcgaggacg gcttctgggt
     1441 ggcggaatgc cccagcctcc ccggctgcat cagccagggg aggagcaagg aggtggcgat
     1501 cgagaagatc aaggaagcga acgaggtata caccgccgct ctcgcggaag acggcatccc
     1561 agttccagaa gatcggctcg acgccatcct ggtggcggta tgatctaagc tctaggcgcc
     1621 gccccccctc ccgcacccaa gaccgccgct gatcaccacc tggccgaggg gctctaactc
     1681 tccatacccc ttctccaaag cgcagaaatg gacccgctat cggctttcgc accgcgtcgt
     1741 tcgctctccc tgccacaaat tatatcagct ttacaacctt tacaatatca tcatgattgc
     1801 aacaaagcgg gtggccgtca ccgaatccgt atgggctgag ctatccgatc tcaagaggcc
     1861 gggagagacc tnntcagcca gctcctcgaa gagatgatag agcgcgagaa gaaggcccgt
     1921 ttgatagcgc atcttaagac gatcgccgat gagggcgact ttgtggagct ccccccttga
     1981 gccgccaggt gatgatcgat cggaaagctc tgaaatacct atcggagctt ccggagaaga
     2041 gccagcggct catcaaggag gagtgccacg ccctctcgga ggacccctac ccgggcctgg
     2101 gagccgataa ggagcttctg catctggatt ttaagctcta tcgattgcat atcggcagat
     2161 ctttcaccgt cttttatcaa atttgcgaga aggacgggct ggtcaagatc ctggagataa
     2221 cgaccatcga caaggcgcac aagctctata agcggcttga ggagtgagcc gccctcttgc
     2281 accgaatatc ccgccttcga tgagggatca tctctcgatc ttctggtccc ccttccgggc
     2341 cctgaggatc accgccccct ccttctcctc cgccccggct gggagccccg cgtcctcgaa
     2401 gatccgaagc cagtcggccc cggagtggtt cggcccgtgg tgatggccga ggggctccac
     2461 cgccagatat cggccccccc tcgccaggac ccgccggatc tcgaagagcc ccttcgggat
     2521 atcctcgagg tggtggagca tgaagaagga gtagaccgtc tcaaaggagc cgccttcgaa
     2581 gggaaggtcg tagacgctgg cggtcctgaa ctcggcgttc tttatcccga gggcctcggc
     2641 gttcatcctc gcctgggccg tcagtgggtc gaagaggtcg atcccgaaga cctccgaagc
     2701 cgggctctgg cttgcgacgt cgagggtcag cctcccaaag ccgcatccta catccaggat
     2761 cctctccccc ttcagctcgg ggaggacctc cggccagacc cgtctccggg ctgagatggg
     2821 gcggggatcg gtgaaggccg cacccatcga ggcagcggcg tcgatggagg tgtaaggcag
     2881 gactaggtag accttcgacc cgttttgggc catcagcccc ctgacgtaga tggcgccgcc
     2941 gccagcccca atccactcga ctatggagac ccccaggcgg ccggagaacc tcctggacca
     3001 ctcctctccg tattccttag gcaacccgtc cagggggtcg tgggcgacga tgtaatgaga
     3061 gaattcgggg ctgaaatacg ccgatggatc ctccccttct ttgagctgga tctcgctgat
     3121 gctgtccttg aagagggcga ggtgctgcag gtcgccggct tcgacgaaat ttcggaccgt
     3181 cccgatctct ccgaacatga tcctccttat ggaaattccc ggataaatag cagtccatcg
     3241 aggggcaaac cccctatagc gcagaatccg gagcttgggg gcgccatcga ctcaccatat
     3301 acggagacgg ggccggaacc cctcggatct tcaggtaagg ccgccctcac aagaactcca
     3361 ccggcatcgc cgcccaggtg aaccccgccc cgctgctcgc gagaaccgcc aggctccccg
     3421 gcccgagcct cccggattcg atcgcccgcg agaggttgaa gagggcgtcg acggtcccca
     3481 tgtggccatg atcccttatc gaggtgatgg tatgatccag gccgaggtcc agcccctgga
     3541 ggatctcctc caggaggctc ctcttcacct ggtttgtcag gaggaggtcg acgtcctgga
     3601 gggagcggcc gctcttctcc agggacctat tgatcacccg gaggtagtta tcgaggtagg
     3661 tctgggagag gatctggtcg aggccggcgg ggtcgtctat ccggaggtag ccgaggccgc
     3721 catccgggat tcccagcggc cgcggccgcc gcgtccctcc gaaggggacc ttgatcgagt
     3781 cgacggcgga gccctccgtc cgggcggcgt aagagaggag ccgatttgtc tcctccccct
     3841 tcttcaggat ggccgcggcc gctccgtccc cgaccatgaa gagggagagg gcgtttctgt
     3901 cctcgtagtc tacggatatg gagagcttct cgctgcagac gaccagggcg cacctcttat
     3961 ccggatcgtc taggaggagg cttttacaga tgctgatccc gagatttccg ccgttgcagc
     4021 cgttctttac ctcaaaggcg tagcagccgt cggcgccgag ggcggcctgg accttggcgg
     4081 cgggagacca gatccggtag tcgtaaaacc cggcgctgca gtagacgagg acgtcgatct
     4141 ctcccggatc gatccccgcc ctctctatcg ccacctctgc ggcccggatg cccatctcgg
     4201 agggatgctc gtcctcagcg gcgacgtgct ttctctccat cccgatcttc tcgaggaaga
     4261 cctccaccgg aataccggaa cgccttgcga tctcttcgct ggtgatgatc cccggcggca
     4321 cgtaacagcc gatggacgcg atccccaccg tctccttatc tcccatcttc gactccgtca
     4381 tatccgcctc actgatctct ccctccccgc gtcgcatctc ccagacctcc gggcggggga
     4441 cccggctctt taagattcat ttcggatccg tcgaagccgc tcccggatca aatgtatgtc
     4501 gctcatcccc cgatcctggg gactgccgga ggtcaccggc cgtcccgacc ccgcccggag
     4561 gagggctcag gcgccccctc ccgccagacg ctctcgaatc ccctgccagt gggttcccct
     4621 ccagtagatc ttcccgcaga cctcgcacct ccagagggcg tcagcatcct cagggccggg
     4681 ggagccgtcg ggccctgatg cctccacttt tcggaggatg ccgttacaga gggtgcacct
     4741 ctcaggattc acctccaggg tgacccccgc ggccagaagc tcccggagct gctcatcgag
     4801 gtccgacgac cttatgagga tgcagctggc cccggccttc ccacacctcc tggcgaggag
     4861 cctgtccctg gtgaggaggg tcctcctctc cttcagggcg acctctagga ggccggagtc
     4921 gtcggacccc accggggggt ttgagacgtc gtggccggcc atcctcagcc agcgggcgag
     4981 ccggagtagc atcaggtctg caagatatcg gtctttcacc atccaccggt ggggacggcc
     5041 gcaactattt atctgtcgat ctacctcttt cggccactat gaagaaggcg atgagcaacg
     5101 tggacgtggc ggcggtcgtg gaggagctgc aggagaagct ggtgggcggc tttgtgggca
     5161 agtcctacca gctctccccc gatagggtgg tgatctcctt tcagtcctcc gcctccggga
     5221 agctcgacct cctcctggag gcggggaggc ggatccacct cacggagaag ccccgggagg
     5281 cccccaagat gccgccccag tttccgacga tgctccgaag ccgcctctcc ggcggccggg
     5341 tggcggccgt ccgccagcac ggcttcgacc gggtggcgga gatcgagatc gagcgggggg
     5401 acgaccggta caccctcatc gccgagatct ttcccaaggg aaacgtcctc ctcctagact
     5461 ccggcggccg gatcgtcctg ccgctgcggc ccctggcctt cagggaccga aagctcctcg
     5521 ccggggagac ctaccagtac cgggaggacc agatcgaccc ccggaccgtc tcccgaaacg
     5581 acctcgcctt catcctcgcc tcctccgact cggagctggt gaggaccctg gtccgggggc
     5641 tgaacatggg ggggacctac gccgaggaga tctgcctccg ggccgggatc aacaagacgg
     5701 agccggcctt cgccctcgcc ggcgaggaga tcgaccggct ccaccgggcc ctgggggagg
     5761 tcttcggcct cgcccaggtg tacccccatc tggtggccga aggggagagg atcgtcgacg
     5821 tcgtccccgc tcccctcgcc gtctacgacg gcctcgaaag gcgggagttc gggtccttca
     5881 gcgaggccct cgacgagttc ttctcctccg atgaggcgga agcagaggag gcgaagccga
     5941 agaccgccct ggagcggaga agggagatgc aggagcggtc gatccaggag tttcgggagc
     6001 gggagcggga gctggcccgt ctcggcgaga aggtctacga gaggtacggc gaggtggagg
     6061 cggtcctcgc cgcgatatcg aagggcttcg agagggggtt cacctactcc gagatcctgg
     6121 cgaagataaa gacctcgggc tttcccatag cggagaagat cctcgccctc gactaccagg
     6181 gggagcttcg cctccgcctc gacgaccccg gcgacgggga cggtggcgaa ggcaaaggcg
     6241 gaaccgtcgg cgataccgga gggaagggcg aggcgagagg agccgttctg gagctgaact
     6301 caaagctcac cgtcccccag aacgcccaga ggtactacga cctggccaag gagcaggcga
     6361 agaagcgaga gggggcggag aaggccctgg aggagacgat ccggctgata gcgcggaagg
     6421 cggggccgga gaaggcgaag acgagggcgg tctatcggcg gaggaagccg aagtggtacg
     6481 agaggttccg ctggttcacc tcctcggacg gctttctcgt catcggcggt agggacgcca
     6541 cctccaacga ggagatctac gccaaatacc tcgaaaaaag ggacctggcc ctccacaccg
     6601 atgcgcccgg tgccccctta acggtcatca agaccctggg ggaggccgtc cccgaatcga
     6661 ccctggagga ggcggcctcg tttgccgtca gctactccag cctctggaag gcgggtcttt
     6721 tcgagggaga ctgctacctg gtggcggccg atcaggtgac caagaccccc gagccgggcg
     6781 agtttctgaa gaagggggcc ttcgtcgtcc ggggcgagcg gcgctactat cgagacgtcc
     6841 ccctcggcct tgccctcggg atcgcgggcg accagctgat cggcggcccc gtcccggcgg
     6901 tgaagccgag ggccgatccc gtcgtcgagg tggagcccgg cgagctcagc cctgaggacc
     6961 tggcgaagag gatctaccgg tccttcgccg agaaggagga tgaccggagg tggctcaagg
     7021 ccatcgcttc cgtcgaccag atcgtgagct tcctcccgcc cggagggtcg agggtgaagg
     7081 ggtgatcgat cgatgaaggg gaggaggaga ttatcctcca atatccccag tcaggaagtc
     7141 ttgttaccgg atatatccgc tttttgttct attttacctg atgaaactgg tctgatggga
     7201 aataatatcc gcatatgaag ctagataatt tattaaccta tgattatccg gctagaatta
     7261 gataataagc ctatttaaga aaattaaaca catataatct tatatcttcg atatcatcgt
     7321 cgatcaaaag atttatctcg gtagaattac agcatatgcc cacgatagac tctcaatcca
     7381 gattcaaagt tttattggat tggccagagg cgatatatgc ttcatttcag gaaatgaaaa
     7441 tattcgcctc agtatagagg tggagaaaga tatataaatt catataggtg gggggatggg
     7501 gacaaatccc cgataatatg ataatacgat catcccaacc ccatatgaag gcatacaggc
     7561 tgaaaagaaa gcagctttgg agtcgaaagg aagattacgg cgttgaagcg gcatgacctg
     7621 atattgaccc tgggatcggt gatgggggtc ttcgccctcc ttccccaggt ttggagcggc
     7681 tatgtgaaca ggaccggggc gatcgagccc gccaccgcct tgatgaacgt cggcatcatg
     7741 gtggcggtgg ggatcaccta ctacgacctg ggcctgcggc ggtcggcggc ggcgatcgga
     7801 gccctcggcg ccctctgggc cgtcctcctc taccagaacg ccatctattg aagcgggtag
     7861 gtctggaggc ctccccacta cctatattaa aaattttaat atactagata tcattctttt
     7921 tgatatcatc cttcttttca taggataccc agcggcacct gttgccaggt gagctgccgc
     7981 aattctacat agtctgctat aatgagtagt tgttacagga tttcgatgat ggaaatcggc
     8041 gatcaggcaa gctttggaat catgatggaa gcgtccattc cgatggtcag tcgcatcact
     8101 ctagccaaac tagaggacca ccatagtccc gatcccgttc gagttcttca acccctgata
     8161 aatcttggga ggcttcctca ggactttcgc catcatttta acgggattcg ctcaattcga
     8221 ggatttggtt tatatagaag ttcaaacaac cagtagttct tagattggta tcgtaattga
     8281 ggcaaatcat ggctggacaa ttcggtggca cacccatata cattctcaga gaaggcagtc
     8341 gacggactgc tggtagagac gcacagagat cgaatatcat ggcggccaag gcggtttctg
     8401 gcgctgtaag gaccactcta ggtcccaagg gcatggacaa aatgctggtg gacacccttg
     8461 gagacgttgt tatcaccaac gacggcgtca ccatcctcaa agagatggac atcgagcatc
     8521 ccgcggccaa gatgatggtg gagatcgcca aaacccagga ccaggaagtg ggggacggca
     8581 ccaccaccgc cgttgtgctg gcaggagagc tgctcaagca agcggagggt ttgctggagg
     8641 aggagattca tccaacggtg atcgcggcag gctacagagc ggcggccgaa aagtccatgg
     8701 aaattctcaa gggtctagct gtaatcgcct ctgcagaaga tgaggatctt ctgaggaaga
     8761 tagccgtcac ggccatgacc ggtaagggct ctcaatcggc ccgggatgag ttagcagtta
     8821 tggccgtcga agcggtcagg tcggttgtag acgaagacgg gaccgtcgat accgacaata
     8881 tcaccgtgga gaagaaggtc ggcggcggca ttacagactc ggtgctcgtg aggggggtgg
     8941 tcatcgataa agagaggctc cagccgagca tgccgaagtc ggtcaccgac gccaggattg
     9001 ccctcatcaa cgcagccgtg gagatcgaga agacagagat cgatgccaag attcagataa
     9061 cttctccaaa ccagctccag gcgttcctgg accaggaaga gacgacgctc aagggcatgg
     9121 tcgataatat cgtcgcctct ggagccaacg tcctcttcgt ccagaagggg atagatgatc
     9181 tcgcccagca tttcctggcc agggccggga tctacaccgt ccgccgcgtc aagaagagcg
     9241 acatggagaa gctcgcacgc gctacaggcg gaaaggtcat caccagcatc cacgatatcg
     9301 acaaagacga ccttgggagg gcgggcctgg ttgaggagag gaaggtcagc gacgagaaga
     9361 tgaccttcgt tgaaggttgc gagaacccca agtccgtatc catcatcttg cgcggtggaa
     9421 ctgagcacgt cgtcgatgag ctggaccggg cgatggagga cgcgctgcgg gttgtgggcg
     9481 tcgctttgga ggataaattg ctggttcccg ggggcggtgc tcccgaggtg gatctggctc
     9541 tccggcttcg ggcgttcgca tccaccgtag gagggcgcga gcagctggcg atcgagtcct
     9601 tcgccgatgc gatggagatc attcccaaga ccctggctga gaacgcaggt ttggaccaga
     9661 tcgattctct catggcgctc cgcagcgccc acgagacggg gatgaagaac tcaggcctga
     9721 atatggagac cggcgagccc gtcgacatgc tgaaacaggg cgtccttgag ccgatcagag
     9781 taaagaccca ggcgatcaac tcagcgaccg aggccgctgt catgatcctc cggatcgatg
     9841 acgtcatagc ctccaagtct gggccaggtg ggatgggcga aatgcccggc ggcatgggcg
     9901 gtatgggcgg agaggacttc gagtaagtcc caccccccac ccatcttccg caacaaagaa
     9961 ggaacgcagg gaaatggata gaagcgatct gttggagttc ttaaaggaga gctggccgga
    10021 gaagttcgca ccggaagaag agatattcag ccatatacac gctggcgaca agatcttcat
    10081 aggcaccggc tgcggcgaac cccagcacct ggttcaggcg ctggtggact ttgtgagcag
    10141 gagccccaaa gcctttttcg gcatcgagct aattcacgtc tggacccttg gaaccgcccc
    10201 ctacttagac gaaaaattcc tcgataactt ccggatcgac tcgttcttca tcagcgaagg
    10261 gacacgaaac gcgatcaacc ggggagctgc agactacacg ccggtatctc tttctgccgt
    10321 tcctggcctg atccgaaggg agattatacc gatagacgta gcgttaatac agacgtcacc
    10381 tcccgataag cacggctata tgagcctggg tataagcgtg gatatcgtaa aggccgcgac
    10441 ccagaaggcg tcattggtcg tcgcccagat caactctcat atgccccgga ctcagggtga
    10501 cggattcata aatataaaag atgt
//
LOCUS       DASM01000181            3541 bp    DNA     linear   ENV 10-OCT-2017
DEFINITION  MAG TPA_asm: Methanothrix harundinacea isolate UBA152
            UBA152_contig_151230, whole genome shotgun sequence.
ACCESSION   DASM01000181 DASM01000000
VERSION     DASM01000181.1
DBLINK      BioProject: PRJNA348753
            BioSample: SAMN06027500
            Sequence Read Archive: SRR5036610
KEYWORDS    WGS; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanothrix harundinacea (hydrocarbon metagenome)
  ORGANISM  Methanothrix harundinacea
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanotrichales; Methanotrichaceae; Methanothrix.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Parks,D.H., Rinke,C., Chuvochina,M., Chaumeil,P.A., Woodcroft,B.J.,
            Evans,P.N., Hugenholtz,P. and Tyson,G.W.
  TITLE     Recovery of nearly 8,000 metagenome-assembled genomes substantially
            expands the tree of life
  JOURNAL   Nat Microbiol (2017) In press
   PUBMED   28894102
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Parks,D.H., Hugenholtz,P. and Tyson,G.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-NOV-2016) School of Chemistry and Molecular
            Biosciences, University of Queensland, Chemistry Bld, Cooper Road,
            St Lucia, Brisbane, Queensland 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: CLC de novo assembler v. 4.4.1
            Genome Coverage       :: 13.60x
            Sequencing Technology :: Illumina HiSeq 2000
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Methanothrix harundinacea"
                     /mol_type="genomic DNA"
                     /submitter_seqid="UBA152_contig_151230"
                     /isolate="UBA152"
                     /isolation_source="waste water"
                     /db_xref="taxon:301375"
                     /environmental_sample
                     /country="Canada: Suncor tailings pond 6"
                     /metagenome_source="hydrocarbon metagenome"
                     /note="Genome binned from sequencing reads available in
                     SRX327722;
                     metagenomic"
BASE COUNT          765 a         1017 c         1109 g          648 t
ORIGIN      
        1 acttcaagaa cgccttccgc ggcgcggaga ctgacctcgc gaccgttgac ggcatctggc
       61 agatctccga cacctacacc gagatcgagg aggacaccga gtatgaccag atgacgatcc
      121 agacggtaga cgccggcgcc aagacgatca tcatggccaa cgaggacaac aagatcaccc
      181 tcagcaagaa gaaggacata tctctgatgg agaacatcag gatcaagacc gctgaccagg
      241 atgacgcacc tcttaggttc tacatctaca aggagatgac catcgagggc gaagaggctg
      301 aggaggccgc accggccccc gtcgaggccc ctgcccccgc agtcgaggag cctgtagctg
      361 aggagcccgt tgccgaggag cnngaggagc ccgtcgctga ggagcccgct gctgaagagc
      421 acgccgagga gcccgctgca gagcccgctg gcggaatccc cggattcgag gcggtcttcg
      481 ccctgactgg cctgttggca gtctcctacc tcgtcctgag gaagagagag taataaagta
      541 tctggaagag cataaagctc ttccatcttt cctttttata aagtcgttta ttatccgctc
      601 tccgctattt tgctcctccc atttcgtagg tcggaagctt tctcgacctg gcatcggggg
      661 cataggcggt gggtggtccg actcgcggag aagaataatt atatgggatt acatctcata
      721 taatttgggg cgatctttat cccgaatatg gatggtgctc ttcatgactg aagccctatt
      781 ctacgataag ctggatggcg gagccgtccg gtgcggccta tgcaaccact tctgcaggat
      841 taagccggag aagaggggga tctgcggcgt cagggagaac gatggcggcg tcctcaagac
      901 cctggtctac ggccggctga tcgccatgca cgtcgaccct atagagaaga agcccctcta
      961 ccacttcatg ccgggccatc tcagctactc catcgccacc gtcggctgca acttccggtg
     1021 cctccactgc cagaacgccg acatatccca gatccccgcc gagggggggg ccatcctagg
     1081 ggagcgggtc gagccggaaa gggtggtggc ggaggccctc agcgctggtt gcaggtcggt
     1141 ggcctatacc tacaccgagc cgacgatctt ctttgagtac gccctggacg tatcgaggct
     1201 ggcggctgat agggggctca aaaacgtctt cgtcagcaac gggtacatga gcgaggaggc
     1261 gacgaggacg atcgagccgt acctcgacgc gataaacgtc gatctgaagg gaaatgacgg
     1321 cttctacagg aaggtctgcg gcgcaaccct cgaccccgtc aagaggacga tagagctgat
     1381 gtggtccctc gggatctggg tggaggtgac gaccctggtg atccccggat acaacgactc
     1441 cgacgaggac ctccgggaga tcgcggagtt cctctcggcg gtgagccctg agatcccgtg
     1501 gcacgtcacc gccttctatc ccacctacag gctgaaggac gccccgccca cgccagcgaa
     1561 gacgatccgg cgggcggccg cgatcgggag ggatgccggc ctttcgtacg tctacggggg
     1621 gaacatcccc ggcgaggacg agaacactgc ttgtgcgaac tgcggcgagc ttttggtgga
     1681 gcggttcggc ttccgggtgg tgaggaacgg ggtcgtggaa gggaggtgcc cgagctgcgg
     1741 cggagcggtg gccggggtat ggtgacccct ggaatggtga tcctctgagg atccggatcg
     1801 gattcttcgg aagatagctt gggccggtcg gcggaggctc ggatggagct agtgatcttt
     1861 taggtctacg gtgaaaatat gtggatggac gagactatac tcggggcggc agctctcggc
     1921 acgaaggttc cagccgtcct ggcagtggca gatgccgaag gtctgagccc cgatcatctt
     1981 ctaaagcagg tgtcaaaagg gcgagtggtc ataatgcaga gggatgagag accgcccctg
     2041 ggcatagggg aggggctcag gaccaaggtc aacgccaacg tagggacctc cgcagaactc
     2101 cccgatcccg aagtcgaggt ggagaaggcc aaagtggcag agagatacgg agctgacacc
     2161 gtcaccgacc tctccatggg aggtcccgtc gatgatattc tgaggaggat atgggaggag
     2221 acgaccagac ctctgaccac cgtccccatc tatcaaactg tagcccagag gggatccttc
     2281 gagtcgatga ccaaggatga cctcctttcg acgatcagag ggcacgtcgc cgccggggtc
     2341 tcctccatcg tagtccacgc cgccatcacc ttcgagatgc tgacggagct cgcgaagacg
     2401 aagaggatca tgggcatcgt atccaagggc ggctccttaa cctcggcatg gatgctgaag
     2461 acgggtgaag agaacccctt cctctccaac tttgaagcga tatgcgagat cctagcaagg
     2521 aaggatatag tcctctctct tggtaacacc atgaggagcg gctgtattca cgacccgatg
     2581 gacaggcttc agaggctgga gctggatatg aactccgacc tcgccagaag ggcgaacgag
     2641 ctgggggtcc aggtgatcat cgaggggatg ggggggcacg tgcggccgga tagaatcccc
     2701 gactacgtgg cacattataa gaaaataact gatcatcgcc ccctcttcgt cgcaggtccc
     2761 ctgccgatag atatcggtgt agggtacgac cacatctccg gatctgtagg gggcgctcta
     2821 gccgccgggg cgggggccga ctacctctgt tacataaccc cctcggagca tctcgccctc
     2881 cccaacgccg cccaggtgag ggaaggggtg gtggccttca ggatcgcggc ccacataggg
     2941 gacactatga agtatggtcc gaggccccag gatgagatgc tggcgaggca caggcgggcc
     3001 agggactggg agaaccagtt ccaattggcc atcgatggtg aaagggcccg ggagaccttc
     3061 catcgagatg gaaccgcgac ctgctccatg tgcgggaaat actgtgccat agccatcatg
     3121 gaggatcatc tcaacggctc ctcttacgcg aatccccaga tctgagatct tcggcctcat
     3181 ctccgaatct gtggtacaga cctcctgggg cggtgagagc aacgacctcc ccatggccgg
     3241 atcagaaatc ccggctgagg atttccggga tcgacgatgg cggtcgaggg gatcgccccc
     3301 tcacctcaat ctaccatcga cgaccatccg ggagcttcgg ggatcagagg acctcttccc
     3361 ggaacttctc aaagccgatc tcgtcgatga aatcgccgag cctcttcttc acggagttcg
     3421 cctggtagta tccgatcacc ttatcgacga gggccatcgc ctcgtcgtcg gagagcccct
     3481 ccgccaggac gaccccgagc cggggcttac gggcggcgtt tcctcccacc aggaccgtcc
     3541 a
//
